LOCUS       DAFEOM010000001       114040 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.001, whole genome shotgun sequence.
ACCESSION   DAFEOM010000001
VERSION     DAFEOM010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 114040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 114040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..114040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.001"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(66..776)
                     /locus_tag="LP524_000001"
     CDS             complement(66..776)
                     /locus_tag="LP524_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HBL2233090.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(773..2245)
                     /locus_tag="LP524_000002"
     CDS             complement(773..2245)
                     /locus_tag="LP524_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBL2233091.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            2383..2856
                     /locus_tag="LP524_000003"
     CDS             2383..2856
                     /locus_tag="LP524_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL2233092.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(3009..3560)
                     /locus_tag="LP524_000004"
     CDS             complement(3009..3560)
                     /locus_tag="LP524_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL2233093.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            3665..4195
                     /locus_tag="LP524_000005"
     CDS             3665..4195
                     /locus_tag="LP524_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HBL2233094.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(4678..5121)
                     /locus_tag="LP524_000006"
     CDS             complement(4678..5121)
                     /locus_tag="LP524_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2233095.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(5137..6297)
                     /locus_tag="LP524_000007"
     CDS             complement(5137..6297)
                     /locus_tag="LP524_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL2233096.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            6804..8504
                     /locus_tag="LP524_000008"
     CDS             6804..8504
                     /locus_tag="LP524_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233097.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(8606..9439)
                     /locus_tag="LP524_000009"
     CDS             complement(8606..9439)
                     /locus_tag="LP524_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBL2233098.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(9743..11908)
                     /locus_tag="LP524_000010"
     CDS             complement(9743..11908)
                     /locus_tag="LP524_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL2233099.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            12168..14462
                     /locus_tag="LP524_000011"
     CDS             12168..14462
                     /locus_tag="LP524_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBL2233100.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFKLLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            14455..15120
                     /gene="pgmB"
                     /locus_tag="LP524_000012"
     CDS             14455..15120
                     /gene="pgmB"
                     /locus_tag="LP524_000012"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBL2233101.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFTKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            15113..16120
                     /locus_tag="LP524_000013"
     CDS             15113..16120
                     /locus_tag="LP524_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBL2233102.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            16142..17161
                     /locus_tag="LP524_000014"
     CDS             16142..17161
                     /locus_tag="LP524_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL2233103.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(17442..18810)
                     /gene="nhaC"
                     /locus_tag="LP524_000015"
                     /pseudo
     CDS             complement(17442..18810)
                     /gene="nhaC"
                     /locus_tag="LP524_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
     gene            complement(18919..20340)
                     /locus_tag="LP524_000016"
     CDS             complement(18919..20340)
                     /locus_tag="LP524_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL2233104.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(20583..22460)
                     /gene="tdc"
                     /locus_tag="LP524_000017"
     CDS             complement(20583..22460)
                     /gene="tdc"
                     /locus_tag="LP524_000017"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBL2233105.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(22751..24007)
                     /locus_tag="LP524_000018"
     CDS             complement(22751..24007)
                     /locus_tag="LP524_000018"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBL2233106.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(24119..24324)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            24587..25888
                     /locus_tag="LP524_000019"
     CDS             24587..25888
                     /locus_tag="LP524_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBL2233107.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(25957..26556)
                     /locus_tag="LP524_000020"
     CDS             complement(25957..26556)
                     /locus_tag="LP524_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL2233108.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            26810..27691
                     /locus_tag="LP524_000021"
     CDS             26810..27691
                     /locus_tag="LP524_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2233109.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            27704..29314
                     /locus_tag="LP524_000022"
     CDS             27704..29314
                     /locus_tag="LP524_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HBL2233110.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(29391..30050)
                     /locus_tag="LP524_000023"
     CDS             complement(29391..30050)
                     /locus_tag="LP524_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBL2233111.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(30333..30998)
                     /locus_tag="LP524_000024"
     CDS             complement(30333..30998)
                     /locus_tag="LP524_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HBL2233112.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(31145..31750)
                     /gene="dhaL"
                     /locus_tag="LP524_000025"
     CDS             complement(31145..31750)
                     /gene="dhaL"
                     /locus_tag="LP524_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HBL2233113.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(31778..32788)
                     /gene="dhaK"
                     /locus_tag="LP524_000026"
     CDS             complement(31778..32788)
                     /gene="dhaK"
                     /locus_tag="LP524_000026"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBL2233114.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(32763..33209)
                     /gene="dhaM"
                     /locus_tag="LP524_000027"
     CDS             complement(32763..33209)
                     /gene="dhaM"
                     /locus_tag="LP524_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HBL2233115.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            33312..33632
                     /locus_tag="LP524_000028"
     CDS             33312..33632
                     /locus_tag="LP524_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBL2233116.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(33744..34613)
                     /locus_tag="LP524_000029"
     CDS             complement(33744..34613)
                     /locus_tag="LP524_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL2233117.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(34709..36013)
                     /locus_tag="LP524_000030"
     CDS             complement(34709..36013)
                     /locus_tag="LP524_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL2233118.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(36010..36519)
                     /locus_tag="LP524_000031"
     CDS             complement(36010..36519)
                     /locus_tag="LP524_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233119.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(36694..38829)
                     /locus_tag="LP524_000032"
     CDS             complement(36694..38829)
                     /locus_tag="LP524_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL2233120.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(38912..40567)
                     /locus_tag="LP524_000033"
     CDS             complement(38912..40567)
                     /locus_tag="LP524_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL2233121.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(40569..40883)
                     /locus_tag="LP524_000034"
     CDS             complement(40569..40883)
                     /locus_tag="LP524_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBL2233122.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            41053..41313
                     /locus_tag="LP524_000035"
     CDS             41053..41313
                     /locus_tag="LP524_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBL2233123.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            41327..41641
                     /locus_tag="LP524_000036"
     CDS             41327..41641
                     /locus_tag="LP524_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBL2233124.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(42371..42925)
                     /locus_tag="LP524_000037"
     CDS             complement(42371..42925)
                     /locus_tag="LP524_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBL2233125.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(43051..44980)
                     /locus_tag="LP524_000038"
                     /pseudo
     CDS             complement(43051..44980)
                     /locus_tag="LP524_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(45286..46002)
                     /locus_tag="LP524_000039"
     CDS             complement(45286..46002)
                     /locus_tag="LP524_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBL2233126.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(46003..47826)
                     /gene="glpO"
                     /locus_tag="LP524_000040"
     CDS             complement(46003..47826)
                     /gene="glpO"
                     /locus_tag="LP524_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HBL2233127.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(47840..49336)
                     /gene="glpK"
                     /locus_tag="LP524_000041"
     CDS             complement(47840..49336)
                     /gene="glpK"
                     /locus_tag="LP524_000041"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBL2233128.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(49472..50932)
                     /locus_tag="LP524_000042"
     CDS             complement(49472..50932)
                     /locus_tag="LP524_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2233129.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(50922..52217)
                     /locus_tag="LP524_000043"
     CDS             complement(50922..52217)
                     /locus_tag="LP524_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL2233130.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(52549..53226)
                     /locus_tag="LP524_000044"
     CDS             complement(52549..53226)
                     /locus_tag="LP524_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HBL2233131.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(53256..54347)
                     /locus_tag="LP524_000045"
     CDS             complement(53256..54347)
                     /locus_tag="LP524_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HBL2233132.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(54448..54882)
                     /locus_tag="LP524_000046"
     CDS             complement(54448..54882)
                     /locus_tag="LP524_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL2233133.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(55466..56923)
                     /gene="ascB"
                     /locus_tag="LP524_000047"
     CDS             complement(55466..56923)
                     /gene="ascB"
                     /locus_tag="LP524_000047"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBL2233134.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(56920..58815)
                     /locus_tag="LP524_000048"
     CDS             complement(56920..58815)
                     /locus_tag="LP524_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL2233135.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(58873..60648)
                     /locus_tag="LP524_000049"
     CDS             complement(58873..60648)
                     /locus_tag="LP524_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBL2233136.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(60698..62194)
                     /locus_tag="LP524_000050"
     CDS             complement(60698..62194)
                     /locus_tag="LP524_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL2233137.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(62388..64010)
                     /locus_tag="LP524_000051"
     CDS             complement(62388..64010)
                     /locus_tag="LP524_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBL2233138.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(64338..65651)
                     /locus_tag="LP524_000052"
     CDS             complement(64338..65651)
                     /locus_tag="LP524_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBL2233139.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(65896..66120)
                     /locus_tag="LP524_000053"
     CDS             complement(65896..66120)
                     /locus_tag="LP524_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233140.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(66140..66562)
                     /locus_tag="LP524_000054"
     CDS             complement(66140..66562)
                     /locus_tag="LP524_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HBL2233141.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(66705..67415)
                     /locus_tag="LP524_000055"
     CDS             complement(66705..67415)
                     /locus_tag="LP524_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233142.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(67433..67930)
                     /locus_tag="LP524_000056"
     CDS             complement(67433..67930)
                     /locus_tag="LP524_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2233143.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(67967..68419)
                     /locus_tag="LP524_000057"
     CDS             complement(67967..68419)
                     /locus_tag="LP524_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL2233144.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(68554..69159)
                     /locus_tag="LP524_000058"
     CDS             complement(68554..69159)
                     /locus_tag="LP524_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBL2233145.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(69609..70229)
                     /locus_tag="LP524_000059"
     CDS             complement(69609..70229)
                     /locus_tag="LP524_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBL2233146.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(70231..71100)
                     /locus_tag="LP524_000060"
     CDS             complement(70231..71100)
                     /locus_tag="LP524_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HBL2233147.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(71123..71530)
                     /locus_tag="LP524_000061"
     CDS             complement(71123..71530)
                     /locus_tag="LP524_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBL2233148.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(71549..72838)
                     /locus_tag="LP524_000062"
     CDS             complement(71549..72838)
                     /locus_tag="LP524_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL2233149.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGDFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(72865..73167)
                     /locus_tag="LP524_000063"
     CDS             complement(72865..73167)
                     /locus_tag="LP524_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2233150.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(73192..75285)
                     /locus_tag="LP524_000064"
     CDS             complement(73192..75285)
                     /locus_tag="LP524_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBL2233151.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(75774..76538)
                     /locus_tag="LP524_000065"
     CDS             complement(75774..76538)
                     /locus_tag="LP524_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HBL2233152.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(76649..77518)
                     /gene="mdcB"
                     /locus_tag="LP524_000066"
     CDS             complement(76649..77518)
                     /gene="mdcB"
                     /locus_tag="LP524_000066"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HBL2233153.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(77543..78619)
                     /locus_tag="LP524_000067"
     CDS             complement(77543..78619)
                     /locus_tag="LP524_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBL2233154.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(78643..79236)
                     /locus_tag="LP524_000068"
     CDS             complement(78643..79236)
                     /locus_tag="LP524_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HBL2233155.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(79240..80916)
                     /gene="mdcD"
                     /locus_tag="LP524_000069"
     CDS             complement(79240..80916)
                     /gene="mdcD"
                     /locus_tag="LP524_000069"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HBL2233156.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(80906..81205)
                     /locus_tag="LP524_000070"
     CDS             complement(80906..81205)
                     /locus_tag="LP524_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HBL2233157.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(81307..82953)
                     /gene="mdcA"
                     /locus_tag="LP524_000071"
     CDS             complement(81307..82953)
                     /gene="mdcA"
                     /locus_tag="LP524_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HBL2233158.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(82960..83871)
                     /locus_tag="LP524_000072"
     CDS             complement(82960..83871)
                     /locus_tag="LP524_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBL2233159.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            83991..84848
                     /locus_tag="LP524_000073"
     CDS             83991..84848
                     /locus_tag="LP524_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL2233160.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(85035..85397)
                     /locus_tag="LP524_000074"
     CDS             complement(85035..85397)
                     /locus_tag="LP524_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBL2233161.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            85743..87182
                     /locus_tag="LP524_000075"
     CDS             85743..87182
                     /locus_tag="LP524_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2233162.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(87189..89741)
                     /locus_tag="LP524_000076"
     CDS             complement(87189..89741)
                     /locus_tag="LP524_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBL2233163.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(89855..91186)
                     /locus_tag="LP524_000077"
     CDS             complement(89855..91186)
                     /locus_tag="LP524_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL2233164.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(91152..92630)
                     /locus_tag="LP524_000078"
     CDS             complement(91152..92630)
                     /locus_tag="LP524_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL2233165.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(92935..94770)
                     /gene="tdc"
                     /locus_tag="LP524_000079"
     CDS             complement(92935..94770)
                     /gene="tdc"
                     /locus_tag="LP524_000079"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBL2233166.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            95240..96667
                     /locus_tag="LP524_000080"
     CDS             95240..96667
                     /locus_tag="LP524_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2233167.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(96759..97124)
                     /locus_tag="LP524_000081"
     CDS             complement(96759..97124)
                     /locus_tag="LP524_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBL2233168.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(97121..97486)
                     /locus_tag="LP524_000082"
     CDS             complement(97121..97486)
                     /locus_tag="LP524_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBL2233169.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(97678..97926)
                     /locus_tag="LP524_000083"
     CDS             complement(97678..97926)
                     /locus_tag="LP524_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233170.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            97925..98053
                     /locus_tag="LP524_000084"
     CDS             97925..98053
                     /locus_tag="LP524_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HBL2233171.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            98110..99804
                     /locus_tag="LP524_000085"
                     /pseudo
     CDS             98110..99804
                     /locus_tag="LP524_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
     gene            99808..99927
                     /locus_tag="LP524_000086"
                     /pseudo
     CDS             99808..99927
                     /locus_tag="LP524_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            100578..101711
                     /locus_tag="LP524_000087"
     CDS             100578..101711
                     /locus_tag="LP524_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HBL2233172.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            101742..102062
                     /locus_tag="LP524_000088"
     CDS             101742..102062
                     /locus_tag="LP524_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HBL2233173.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(102091..102555)
                     /locus_tag="LP524_000089"
     CDS             complement(102091..102555)
                     /locus_tag="LP524_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2233174.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(102687..103941)
                     /locus_tag="LP524_000090"
                     /pseudo
     CDS             complement(102687..103941)
                     /locus_tag="LP524_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(103970..104287)
                     /locus_tag="LP524_000091"
     CDS             complement(103970..104287)
                     /locus_tag="LP524_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBL2233175.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(104313..104633)
                     /locus_tag="LP524_000092"
     CDS             complement(104313..104633)
                     /locus_tag="LP524_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2233176.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(105097..106512)
                     /locus_tag="LP524_000093"
     CDS             complement(105097..106512)
                     /locus_tag="LP524_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL2233177.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(106529..107845)
                     /locus_tag="LP524_000094"
     CDS             complement(106529..107845)
                     /locus_tag="LP524_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL2233178.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(107995..108693)
                     /locus_tag="LP524_000095"
     CDS             complement(107995..108693)
                     /locus_tag="LP524_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBL2233179.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(108982..109938)
                     /locus_tag="LP524_000096"
     CDS             complement(108982..109938)
                     /locus_tag="LP524_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBL2233180.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(110051..110884)
                     /gene="msrA"
                     /locus_tag="LP524_000097"
     CDS             complement(110051..110884)
                     /gene="msrA"
                     /locus_tag="LP524_000097"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBL2233181.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(111059..112687)
                     /locus_tag="LP524_000098"
     CDS             complement(111059..112687)
                     /locus_tag="LP524_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233182.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIRLVSKGFKNADFIAI
                     FKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNNDL
                     TTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAKLL
                     QFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFNQK
                     IISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNLPY
                     KTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGVAD
                     IKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(112674..114008)
                     /locus_tag="LP524_000099"
     CDS             complement(112674..114008)
                     /locus_tag="LP524_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233183.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT        33561 a        23812 c        19633 g        37034 t
ORIGIN      
        1 ctgattgttc ttactgcttg atgcagagca gctttttcac aacctcctac atggtgttca
       61 aaatattatt cgccttttgc ttctttcact aattttgcga atgcttgttg ttggaattca
      121 gcagctaagg ctttcgtacg ttgttcttgc tctgctttgc tcatttttgg attttcagtc
      181 attgctttta ccataccgtc ttgaacataa gcttttgctt cactttccat cgtactttgc
      241 tgtttcttca ctaattcagc taacttggct gcttggtccg tacttaattc tacccatgtc
      301 tctgggattt tgaatgtatt cactcggctg tcatattcgt gattgttatc agcaatattg
      361 aaccagaatt tatacatgtc gtttttgtat tcccaataag tagttttcgt cactttttca
      421 gcatcgccgt ctgtcttttc tactttattt gtttcgttat ccgtaccagt tgcttttggt
      481 ttttcttgtt tttcatttgt tcggtaaaga tacacttttt cttttccatt ccctaaagct
      541 ttatacaaaa gcatatctgc tccttgggaa tcagcactgg aaacaatttt ttctgttttt
      601 gtttccgtta ctttttccat tccaaagtga ttggacaaat tagcgacgat caaacttagt
      661 gaagcgacga agattagtcc gaaaatcaaa gataaaaagg tttgccatgt ttttttagcg
      721 aacacgttcg ttaaagcaaa agcaagcaag ctgacaatca aaactccaat aatcatgcat
      781 tatcccccgc tttcattaga acaggttctt ttttcttcaa taaaaaggca ataataaagc
      841 caagaatccc aaagatgatc gctacgataa acgcggcatg gtaacctgac agtgtggcat
      901 tgattgcctg atctttataa gctaatggcg tattatccaa tacagatttg cttgggatat
      961 ttgcttttgt cacattcgtc aacacactga tcaaaatagc tgtaccaatc gagcttgcga
     1021 tttgacggaa tgtattattt accgctgtcc catgactgat caagttattt ggcaatgcat
     1081 tcataccaga agtcgttacc ggcatcatga ccatggagat cccaaacata cggatcgcat
     1141 aaaggatcac gatatataaa actggagtcg tttttgtcaa gaaagcaaat ggtgcagttg
     1201 ccgctgtcaa aatgaacatc cctgtgatgg ctaaacgttt cgcgccgtat ttatcgaaaa
     1261 tcgcacccgt cacaggcatc atagccccca tgatcaatgc acccggcaac aacatcaagc
     1321 cagaatggaa tgctgattct ccacgaatat tttgaatata caatggtacg accatttctg
     1381 cccctaccat cgccatattt gttacaccag ctaaaacagc tgcaatcgtg aatgtagatg
     1441 atttgaatac acgaagttcc aagaatggat gttccaaatg taattgacgc catgagaaca
     1501 aggcgatgaa aatcacacca acaactaaga aaccaattac tttcaagctt ccccagccgt
     1561 catctcctac actagaaaat ccgtaaagta aactaccgaa tccaatcgtt gataaaatag
     1621 cggataaaat atcaatgcta ggatttgaca atggaataac acttttcatt aggaagaatg
     1681 ctaaaaccaa gacaagaaca acgattggca gtaccatacc aaacaagtca cgccaatggt
     1741 agttatcaat gatataacca gataatgtag ggcctaaagc aggagcaagt ccaataacga
     1801 tccctgacat ccccattgct gatcctcttt tttctggcgg gaaaatagat agcatgatat
     1861 tttgcaataa tggcatcgat actcccacac cgcaagcttg gatcaaacga ccgattagta
     1921 atgtactaaa attaggtgct gtaaagcaag taatcgttcc gatcaaaaag atcgtcatgg
     1981 cagaaagata taaatttctt gaactgaatt tattgatcag ccatgctgtt attgggatca
     2041 tgatcccgtt gactagcaag aaaccagtcg ttaaccattg cacatcagac gctgaaatat
     2101 cgaaagcttt catcaatgta ggaaaagctg tcgtcaataa ggtctgattc aaaacggtac
     2161 aaaatgtacc aattaataag acaacaacta aaagtgtccg attatatggt tttccgtaaa
     2221 tatctacggg ttggttgtta ctcatatttc tccttctttc tgaatttttt ctgaaactac
     2281 tagaatactt tagtcctaaa ttcgctattt gtcaacctac ttgacacata agttaatacg
     2341 gatataatta agggaagcaa gaggacaaaa ggagtgacaa aaatggttgg ggcttccctt
     2401 aaacgtttgt tggaaaatga tcaattggtt gttggcatca gtgaattaag tgatatcgta
     2461 ggtgtgtctc cacgtcaatt aaggtattgg gaacaaaagg gattcattcg ttcgatcgaa
     2521 gctgatgcga attgtcctag aaaatatcgt ttacctacag tcataaaagt agaactgatc
     2581 aaaaaatatt tagatgacgg atttactttg tcaaaagctg cagaaaaggc agaaatccgc
     2641 caaaagaaaa tgcaccatgt ccgtaaagtt ttttcaaaag caatcaaaga tatcgaattg
     2701 atcgacgaac gctatacgat tttacaaatc ggtaacttca aagaagagaa tgctaagatg
     2761 tacatcattc atgacaacga gacagacgaa ttaacttaca agctccttcc tgtcgatgaa
     2821 aaagctgact acgaacagtt gttaaaagaa ctctaaacgc cactaatctc ctaagtacca
     2881 agctttttaa taagtagatg ctacataaat acttgaaaaa aggcctgttt gaacataaga
     2941 atatccgaac ggtgatctgc gtttctttca tatagaaagg aattctccat cactgttcgg
     3001 atattttttt atttttttcg gttactttct tctaatacat ctgttagcca atttgcatat
     3061 tctgtttccc gattgatcgc atgcgctaaa atcaggtagt gtccgtaatt ttcatgtttt
     3121 tctttttcag taggaaagat cgtttttttt cgctctaata gatgaagtcg tttttcttca
     3181 tgaaggtcac gctgctgctg aataatttct actaatgccg gatcattcac gtctttgacg
     3241 aaatacaatt tcagaacaaa ttcgtctcga ttaaccggta gttcattggt gggtgtatga
     3301 atccactctg ccaattgttc cttcccttct tctgttaatt cataaacttt cttttccaat
     3361 ttttcacctg tgatctctac ttgatgctga atgattcctt gctcttccat ttttgcaagt
     3421 tcaggataaa tctgactatg ttttgcttgc caaaattctc ctatttcatt tttgaacgat
     3481 tgattcaatt catatcctgt catcttcttc ttacttaata accctaacaa aatatatcga
     3541 agtgtatttt ttcttgccat cagttttccc tccttgacaa atctttaaat ccattctatc
     3601 ataaatgagt aatcgaaaac atgtagttta ttacatgttt taatttataa ggagatgctt
     3661 aaacatgaaa gaatttaaag aattggaaga ttttattgga actcacttta tctatacgta
     3721 tgacaatggt tgggaatatg aatggtatgc caaaaatgac catactgttg attaccgtat
     3781 ccatggcgga atggtcaaag gccgttgggt aaaagaccaa gaagctgata tcgttaaatt
     3841 gacggacggc gtattcaaaa tcacttggac agagccaaca gggacagacg tggcattgga
     3901 ctttatgcca aatgaaaaga aacttcacgg aatgatcttt ttccctaaat gggttgaaga
     3961 acatccagaa atcactgtta ctttccaaaa cgaacatatc gcagaaatgg aagcagctcg
     4021 tgaaaaatat gaaacttatc caaaattact tgttcctgaa tttgctacga tcacttatat
     4081 gggtgatgca ggtatagaca atgaagatgt tattagcgaa gaaccatacg atggaatgcc
     4141 agatgatatc cgtagcggaa aatacttcga tgaaaattat aaacgagtaa aataaggttg
     4201 tgaaagagag gttgtgaaat cagcgttctg acctgagtat tagagaaaga aacggaaaaa
     4261 tagttcctca tgttttgccg ttttttgagc taataccgag ggtcagctga ttgaacaccg
     4321 tttatcaaga ggttgtgaaa tcagcgttat gctttgagta ataagaacgc tagttagaaa
     4381 atagcttttc atattttcta ggctagtggg cttattaccg agaagcagcc gtttgaacac
     4441 cgtttatcaa gaggttgtga aatcagcgtt ttgacctgag tattagagaa acaaatggaa
     4501 aaatagttcc tcatattttg ccgttttttg agctaatacc gagggtcagc tgattgaaca
     4561 ccgtttatca aagattgtaa caactgcata ttcacacagt taaattagtg acttcaaaat
     4621 gaaataatgc tcccagcaaa aaattaggct gggagcatta tttcgttatt ctgttcatca
     4681 aaaatcagcg ttttgattcc atttcatcac aagttcctcc tcatttactg cttcatcttt
     4741 ttgctgtgtt tgaattgtaa aaccttgtga aagatagaaa tgaatagctg attgattttt
     4801 ttgatagact gtcaaagtca gtgttttatt ggtttctttt atttttttca ttagttgcgt
     4861 accgattctt tgttgccggt attccctttt cacaaagatt cctgcgataa actgatcaat
     4921 caccccagca aaaccaataa tttcttgatc tatttctgct aagtaaatca ctgcatctgg
     4981 taaagctttc ctaacagatt ctacttgatt ctcccaataa gttgatggaa tgaattcatg
     5041 agaatccaag ttagcatgca gccaaatcga tagaacagat tccagttctt tggcagacaa
     5101 ttttgttgtt gtgcggatca ttttttctct ccttacttat tcattgattt taaaataaaa
     5161 accatttttt tgaagctctt ttacaagttt tcgcccctca aaaaagtata tcttctcttg
     5221 ttttactttt ctaataccaa aagaaacagc aactttagga tcatcagctg aaacgattcg
     5281 ataagttcca ccaggcaatt gatccagctt gactttccca tgatggtcgc tttgtactga
     5341 aaacggcaca ccatttctag taagtgtttt tttcttatgt ttcaaaatga atgaactacc
     5401 atttaccggt ttccctgacg aatcactaat aataaatgaa aaatcaagca cttttttact
     5461 taatctatac tgatagatta gtcgtccgat aaacactaac agtatgacta cagctattat
     5521 cgttcctgca agaataaacc attgccgcat ggttctttcc tgatttcctt ttccttcttc
     5581 tttttttacc gattcgttat aaggaatccg atgtcctgtc actagcaaac gatgcgtgtt
     5641 gatcatatac ggcgtacatg taagcaaggt aactaagtct tctccttctg aaatcgttag
     5701 aacagacgtg tcatctggca gtaccacttg tatattttta acttgataag ccaatttctc
     5761 ccccaaaaca gtcaaaataa acgtgtctcc gttttctaaa cgatctaatt cggtaaataa
     5821 ccgtcgatta ggtaaaccgc tgtgagcaga gatcactgaa tgcgtcgaat ttccaccaag
     5881 cgggaaagag gttccttgca agacgcctgc tccattttca aggatcctac cattgagccg
     5941 atcataaagt gaaatattta attggatctt aggtatattg atacttccga tcagccattc
     6001 ctctaattgg ctggatgttg tactttcatt tctgttcttt tcattgaaag gatcagtacc
     6061 aggattgaat ccttggtcag ttaatcgttg gtttttctcc tccattttct ttcgctgcac
     6121 ttcaatttca gctgcggtcc gtttttgtac ttgttcgata cgtttttgat caataaaact
     6181 atttaaagaa tccacataaa atggatataa agcgatcagt aaccctacga cgatacatat
     6241 taataaaaac aaacgaaaca aaagtcgacg agactgtttt tgcttactct gcttcacatg
     6301 tgtcctccct tgcaagtttt tttgttcttt cttttattat atgggttcag gaaaaaaata
     6361 gaaaatgaaa gccgatgagg ttgtgaaaaa gctgtcctgc atcaagtaat aagtacagcc
     6421 caaaaaaata gctactcata ttttttgttg ggttgggctt attaccgcag atgcaagctt
     6481 ttgaacaccg tttaaggaag aggttgtgaa aactgcgtta tgctttgagt aataagagcg
     6541 ctagtcagaa aatagcttct catattttct aggctagtga gcttattacc gagaagcagc
     6601 tgattgaaca ccgtttaccg agaggtcatg aaaaagctac tttgtttcga gtattaagaa
     6661 aaattctgtt tcaaaacaag gagatgcaac aaaacttatg tcgcatcctc tctccttaga
     6721 actttcactt atgtttttct tgtaaaattc ataaatattt tcatgaattt tctaatttct
     6781 tgcctacctc tcgcatagga tatatgatga atttatctaa agtttgcaaa ggagttcgac
     6841 aattgaaaaa atatggccca tttttagcaa tttttttcat tgctttggca tttctcatgc
     6901 ctcatttatt cacacataaa ctaattttgg gtgctgatgc cccattccat tacaatcggt
     6961 tctatgatgc cgcgcaacaa attaaatatg gggatttcca ttattttcct tccctttatg
     7021 gattccagca aagtggcagg atcgtgaatg ccgtttacgg cccatttttc gcttatttac
     7081 aaggattgat cgtgttgctt gcaggtagct ggtttcatta tcagattctt tcaaatttca
     7141 ttctttatct tatttcagga ttttctatgc tgattttact gagatatgtc aatgttcgaa
     7201 attgggtaag tgtaccagta gccgttttat tcatgagtac gtattctatt caatactggt
     7261 ctatcaacca aggattcact agctggggga cttgttttta tcctttatgt atgatcccta
     7321 ttgctgattt cgtccttcat aaaaagttcc ctgttatcaa agtcgctgtc tcagtgggac
     7381 ttatgacaca gatccattta ctgtcttcga tcatgttagc tttgatgtat atcccttttt
     7441 atctttatta cttttttaac cagactgaaa aaggaaaagc actgcttcaa ctgattaagt
     7501 cggtcttgct ttattttgtt ctgacagcaa atatttgggc aggattagct attgtttaca
     7561 gtgggaataa gattcttcct ccctttgtta acagagaaat gagtcaaaag gcgatagatc
     7621 tggatggcag ttattggctc cattacccta aggcctttcc tatattgata ttatcaggtt
     7681 ttttgctttt tattttcttt caacaaaaga acaccttatt ccaaaaaatc gtgttgtaca
     7741 caagtcttgt tttctttttt ctttctacta gcttgattcc atggacgaca ttgatcgaaa
     7801 aaaatgtgcc ttttattagt ttgatccagt ttccatttcg atttttcgtg ccatttacgc
     7861 tattgcttct actttacctt gccctttcac tgaaccagtg gtctgaacaa aagtggttcc
     7921 gtcttttaag tatatttggc attcttattt tttcagcaca gacgatccag aacttatacc
     7981 agcatctgga aaagtgggaa aatgaaacat ttgtcagtcg acatacttat ctattcgata
     8041 cgccggaaga agcaaggaaa acattcttca gcaaagatat gtctaccagc ttgttcgttt
     8101 tccaaaaaac cacacctgac tatctgccaa tctataagga gacaaaagac aataaatacg
     8161 accggtataa cgaagaaata ctagaaaacg aaacaacttt tataaaaaca catccaggcg
     8221 gtcttttgac aatcaagtgg aaaaacactg gggacaaaac tgttcatatc cctgttatcg
     8281 tctacgatcg gactgtgcta cagcaaaatg gaaaaacatt gactgattat gaagtaacag
     8341 atatcgggac cccaatcgtg aagcagcaaa aaggaatcaa cgagctcacg cttcattatc
     8401 aaacgccaat ctatttctat ttcattcttt ctcttacttt gattggctgg tttaccttgc
     8461 tatgtttatt tatttatcat agatacaagc ttttacgtgc ttaggcaaaa gggataaata
     8521 catccgatca aaaaactctc caaacggtat aatcgtttgg agagttttat tttaatcagt
     8581 ttttttatgt tatatgttat attttttatt cgttcacatc taattcgatt tccaccccgt
     8641 agtgatcact gatgattgga ctatcgatcc catcaaaaat ccgttgatat tttttaacct
     8701 gcattccttc agggacgaaa ataaaatcta tacgcaattt ttctgtattt tgttcccaac
     8761 catctatttt cttttccaca gtagctgctt ttcctttttc attcgcgaca acatacgaat
     8821 cttgaagaag aaggcaactt tctcttacag tataataacc ttgagagtct actggattat
     8881 tgaaatcacc taggaaaaaa agtggtacgc gagtttccaa aaagtagttt tccaagtttt
     8941 tccactcaaa ataaaagcca ctttttttat ctgtccacca agaaaagtgg cagctcgaga
     9001 caaaaatgtc ctgatcatct atctttgttt tcccaatcaa gatcttgcgt gtctgataat
     9061 cttctttctt ttgaaattgg gagacggtaa ggacctgact ttccaatgtt tttttcgtaa
     9121 gcagtgcatt tccttcttca tatttatcat aaccgatgtg gctcatttcc caactccaaa
     9181 aatactcttt tccgtgttcc tttaaaagct gaactaaacg gtaggcaaaa ttatctttac
     9241 ggataggtac ttgattcttt accggacaga acttcattag cttcgcaggt tctacttctt
     9301 cagattccag taattggttg atttcttgta aagcaatcac ttcataatct tctaacagga
     9361 tcttatctgc taattcttgc aattttttct ctggttctgg ctcaagccag ctatgcgtgt
     9421 tgagtgtcat gaatttcatt ggttttctcc ttgtcttcat ttcgaaacaa aagggttgtg
     9481 acaaaatttt tgtcacaacc tctccagcaa atagtgttct cctgctaact cctcagtaat
     9541 aagctcaatg gaccaggagc tattttttgt ttggctgttt ttatcacttg gtgcagggca
     9601 gctttttcac gacctctcca ctaaacagta ttcaaactgc tgcttctcgg taataagccc
     9661 aaccaaacga aaaataggag gagctatttt tgtttggctg ttcttatgac ttgatgcagg
     9721 acagcttttt cacaacctct ccttacattt taatggagcc gatcacttta ccagctgtca
     9781 ctgtacctaa ttgcttgatc actacttctt ctactttatc gtttgtaaat acgatcatga
     9841 tcgttggatc tttttcttcg ttttggatca catcaagatc catctcagct aatttcgttc
     9901 ctgttcctac ttcttggcct tctttgacag caatctcaaa agctggctga ttcatttgga
     9961 ctgtatcgat tcccatatgg atcaaaactt cgataccatc tttcgtttcc agcccaattg
    10021 catgtttgct agggaaaata cttgtcactt ttccagcgat cggcgcaaca acttctcttg
    10081 atgttggaat aacagcaaag ccgtcaccca tcattttttg tgagaatacc ggatctttta
    10141 cttcagataa aggaatcact tctccagaag ctgcggaaac tagttcttgt tctataccta
    10201 agtattgctt actacctgct tgtccatttc ctgtagaaac catttctggc tctggaatct
    10261 ctgctcctga ttgcagcaga tcttcgatat ctgatttcaa gacatctgct tttggtccat
    10321 aaactgcttg gactccattg tctttgacga tcaatcccat cgcccctgct cgtttccagg
    10381 cttcttcagt ccctactttt tctcgatcat ttacacttac gcgtaaccga gtcatacaag
    10441 catcgacatc tttgatattt tcttttcctc ccagcaaatg aatgatctgg accacttgct
    10501 gatctgctgt tcctgttccg ctgttagata caacggtttc ttcactgtca ttgtcgtaat
    10561 ttccgttacg tcccggtgta gcaaagttga attttttgat caagaaattt gctaagaaat
    10621 acgtaacaac accaaaagcg atcacacaca acacgaagtt gataagatct ccacctagac
    10681 ccgctttgat agctaacggt gtccgagtca gcaattcgat attcccaaat gaatggaccc
    10741 gcaatggcaa gatatctgcc atcgcaaatg cagccccttg gatcactgaa taaatgacat
    10801 ataatggcac agcagcaaac ataaacatga actccaatgg ttctgttaca cctgttagaa
    10861 agacagccaa cgctgctgaa aaatacattg atttatatct tgctttctta tcaggatcga
    10921 cattacgata catcgcaaaa gccattccca tcaagatacc tgaagaacca atcatttgtc
    10981 ctactttgaa acgagccggc gtccagttat gcaacacaaa ctcatattgc gacatatccc
    11041 ctgcaccttt aagattcact aaatcagtgg cccaagccaa ccataacgga tcttgcccaa
    11101 atacttgtgt ccccgcctgt gcgcctgata aaatctcata cgatccgcca agctgtgtat
    11161 agttgatagg aatcgtcagc atatgatgca atccaaatgg caatagtaaa cgttctaatg
    11221 ttccaaataa aaacggtgct agaatcggtg cgctttcttg tgattctgca atccataaac
    11281 caaaattatt gattccagcc tgtacgtttg gccacaccac agcaagaatc aatgccacga
    11341 ttgttgacca taagataaca acaaacggaa caaagcgctt tccattgaaa aatgacaaag
    11401 catccggcaa ttttctataa ttatagtact tattataagc cattgccccg acaaatccag
    11461 cgatgatccc tacgaatact cccatattca aagctggtgc ttccaataca cttgtaaaaa
    11521 atcctttaac catgatcttc gtaccgaaca gagtgtgggt aaatgcatct tcattcgcaa
    11581 gcatgtcact tgtgacacca aagatagccc cagtgatccg attgattaaa ataaatgaga
    11641 tcccagctgc aaatgctccg cctgctcttt cttttgccca acttcctcct attgctaaag
    11701 caaataaaat atgaagatta ccaataattg cccaaccaat attttcaatt actccgcccg
    11761 ttgtgacaag cggcgtccaa tcaggattga tcaagggaat tgatttccct atactgatca
    11821 tcaacccagc tgccggcata actgctacca caaccattaa agcctttcca aatttttgcc
    11881 aaaactcaaa actaaatatc tttttcattc cgtttagctc ctttacgcaa tcggtttcgt
    11941 taaacgtatt atagcgtgcg ttttcttttt tgtaaatgct ttatttttta tttttctgtc
    12001 tataccattc tataaaacct ataatacgtg tgttcccttc tataattttc tcctaaaaat
    12061 ttattttttt aagctttttt ctatgaaata ttttcttttg tctattgact tgtgcaatcg
    12121 gttgcgttta ttatgaacgt ataaaacagc tcattaggag gagaaaaatg aaacagatca
    12181 aacgactatt tgctattgat ccatggaaaa tcagcacgac acaattagat aaaaaaaatc
    12241 ttcgactgca agaatcttta acaagtattg gaaacggtta tatggggatg cgcgggaact
    12301 ttgaagaaac ttactcaggg gaccaccatc aaggtactta tttggcgggt gtctggtatc
    12361 ccgataagac acgtgtcggc tggtggaaaa atggttatcc agaatacttt ggaaaagtga
    12421 tcaacgcctt gaactttatc gcgatagata tttcgatcaa cgatcagctg atcgacttgg
    12481 cttctttgga accagaagac ttttactggg aattagatat gaaaaatggt gtattatctc
    12541 gtacattcac aatcactact tctactaata aagtacgcat ttccttcgaa cgttttctga
    12601 gcattgtcaa aaaagaatca gcttatatct atttaactat agaaatgctt gaaggtagcg
    12661 gaacagtcaa actcgtatca aaattggatg gaaatgttca aaacgaagac agcaactatg
    12721 atgaacattt ttggaatgag attgatcgtg gaactataga agaaatcggc tatttgacga
    12781 ctaaaactat tccaaacaac tttgggattg aacaatttac tgttactgca gcaatgcgcc
    12841 atttcgttaa tggaaaatca gtcatccctg actatcacga agatacttta gcattacatg
    12901 gttgcttgtc tgcagaatta tctcaaggtg agacatggtc agtggaaaaa gaaattatcg
    12961 ttcttactag tagagatatt ccagaagcgc agcaagcaga aaaagctgtc tcagaattaa
    13021 cgaaccagac aaacaattat atccaagcaa aagaagagca aacagcggct tgggcagaac
    13081 gatggaagct tgccgatgtg gtcatcgaag gagacgaaga agcacaacaa ggtattcgat
    13141 tcaatttgtt ccaactattc tctacttact acggcgaaga tgaacgattg aatatcggcc
    13201 ccaaaggatt cacaggagaa aaatacggcg gagccactta ctgggataca gaagcgtatg
    13261 ctgtccctct ttatcttgct ttagccaaac ctgaagtaac taagaatctg ctgaaatatc
    13321 ggcataatca actgccgcaa gcaatccaca acgctcagca acaaggatta aaaggcgcgc
    13381 tttatcctat ggtcaccttc actggtgtcg aatgccataa cgagtgggaa atcacattcg
    13441 aggagattca ccgaaatggc gcgattgcct atgcaatcta caattatacg aattacaccg
    13501 gcgatacgga atatttgaaa aatgaaggac tggaagtttt agcagaaatc gcacgcttct
    13561 gggcagaccg tgtccatttt tcaaaacgag caaataaata catgatccac ggtgtgaccg
    13621 gcccaaacga atatgagaac aatatcaata acaattggta tacaaatacg atcgcagctt
    13681 gggtcttgcg ttacacgcga gaaaattatt taacttatca aaaagaagct gctatcacta
    13741 tcagcgaaga agaactagcc aaatggaaag atatcgcaga gaacatgtac ttcccagttg
    13801 acgaagaatt aggaatcttt gtccagcatg acacattctt agataaagat ctcatgcctg
    13861 tatccgatat tcccacaagc gatcttcctc tgaatcagca ttggtcttgg gacaaaatcc
    13921 ttagatcttg cttcatcaag caggcagatg tactacaggg gatttatttc ttcaatgata
    13981 cttttacaaa agaagaaaag caaaaaaact ttgatttcta tgaaccaatg actgtccatg
    14041 agtcttccct ttcgccaagt atccattcga ttttagcagc agaattagga atggaaagca
    14101 aagctgtaga aatgtaccag cgaacagctc gcttggattt ggataattac aataacgaca
    14161 cagaagacgg cttgcacatc acttccatga ctggtagctg gttgtcgatt gtccaaggat
    14221 ttgctcaaat gaaaactgcc catgatcagt tgagattcgc accatttctg ccaaaaactt
    14281 ggaaagctta ttctttccat atcaattatc gcggacgttt attgttcatc tcagtcacag
    14341 atgaacaagt tactttcaag ttattagaag gttctcctct agaaatttac gtctatgaac
    14401 agtcttattc tttagcggat cgtctagaaa taacgctaag aaaggaggaa gcaaatgttt
    14461 aaaggggtat tgtttgattt agacggtgtc attaccgata ctgctgaatt ccattatcgt
    14521 gcttggcgaa agcttggtca ggaaatcggc attacgatcg accacacatt caacgaacag
    14581 ctaaaaggcg tcagccgaga agactcactc tctttgcttt tagcttatgg cggaaaagaa
    14641 cactctttta caaaggaaga atttgcagaa ttagcgaaaa gaaaaaacga ttattattta
    14701 gagatgatcc agacgatcga accaaaagat gtcttccccg gggtcgtgcc tttactggat
    14761 tctttaaaag aagaaaaaat caagattgct cttgcttctg caagtaaaaa cgggccattc
    14821 ctacttgaaa aaatgggtct tacaccttac tttgatgcta ttgccgaccc ggcagaagca
    14881 acaaacggaa aacctgcgcc agatatcttc ctccttgcag caaaagctgt cggactaaaa
    14941 gcagaagaat gtatcgggat cgaagacgcg caagccggta tccaagccat cctctcaagt
    15001 ggcgctcaac caattggcgt gggacgtgct gaagaccttg gagaggagat cccgcttgtc
    15061 cctgatacga cttttcttac gatcgattac ttgaaagaaa agtggcatga ccatggataa
    15121 tccagagatc tccgtcgtac cagttccaga gactgaaata aagaaaatca cattgaagaa
    15181 cgactactta gaagtcgttc ttctcaatta cggtgcccgc ctgcaccaaa tcttcgcacc
    15241 agacaaagaa ggaaaatcag aaaatgtttt attgtcttac gatcgtttcg aagatgttct
    15301 aacagacaaa agcttttttg gagcaactgt cggacctgta gccggaagga tccgggatgc
    15361 ttcttggggt gagcatagat tggagaaaaa ctgcggatca caccatatcc acggtggcac
    15421 gaaaggctgg tctttccaat tttgggaagt agaagttttc aaaactccat catcaatagg
    15481 tgtggtcttt tacttaaaag atgaattttc tacctatcca ggaccaatca ctgctacgat
    15541 cacttaccgt ttaacaaaaa acgaattaga gatgacaacc tctgctagta gccttgttga
    15601 aaccatctgc aatccgacca accatgccta cttcaacctt tcaggaaatg gaacaagaga
    15661 catctatgaa catcagttaa cggtcttttt agatggtgtg cttgaattag atcaagaaaa
    15721 actgcctact ggaaattgga caaaaaaaga aaaactgcca atcgacttta gaaaatcccc
    15781 aacactgcaa gagatccttg catgttatcc agatggacta gatgatgtat ttcttttgca
    15841 tcatcctagg ctttccaaaa cttccttaca attattcgag aagcattctg gtcgccaaat
    15901 gactatagca acttctaata aaagtatggt attattttct acgactggat tcgaagctga
    15961 tttctctgtc aatggaaaac agatgcattc aaactacgga ttagcaatcg aaccgcaaga
    16021 aatccctgat atcgttcatt tccccaaatt tggatcgatc aacctccatc ctggtcaaga
    16081 acggatcagt cagacgattt atcggttctc tgcacaataa caagagaaag gtgtttttat
    16141 catggctatt accgtaaaag atgtggcgaa aaaagccggt gttgccacct cgaccgtctc
    16201 tcgtgtcatc aacgatcatc ctagtatttc cgagacaaca aagaagaaag tccgaaaaat
    16261 tatggatgaa ctaggttatg ttcctaacat gactgcccgc aatctaggaa aacgcatttc
    16321 cagtgccatc ggcgtaatct taccccctct tgattcaaaa gagcggatcg gtaacccatt
    16381 ttatcttgag atcatggaag caatcaacga agaagcgcgg aattatgata tgactacagc
    16441 aatcgctact gctaaaaatt ttgaattgct gttagagaac gtccagcgta tgcatttaca
    16501 aaaacaagta gatggtttta ttctagtgta ttcagatagt caagatactg tcattgatta
    16561 tttataccaa aaccacatcc ctttcacttt gatcggacag ccttatcatc atgagaacga
    16621 aatcgtttat gtagataatg ataaccagct tctaggtaaa caagcaacag aatttttgat
    16681 ccagaatgga cataagaata tcctgtttgt gacaaacacg actcatgaaa gtttgtattt
    16741 cgaacgatat ttcggttatc aaaaagcaat gatgatggct ggtcttcctg cgttatccag
    16801 tgtagtacta gagcatccgg aggattatct gtcctttgag aaaacgttga aagaaaacga
    16861 agccacagca ttggtggtca ttgacgatat cttcgccctt cgcacgatgc agttggcgca
    16921 aatgtatggc tatgtcattc cagagacact ctccgtcatt agcttcaaca actcgatctt
    16981 ctcaacatta acacaccctt accttacaag tatcgatatc gatatctccg aattaggacg
    17041 tgtcgctatg caaaagctgc gcgaattgat ccatgaagaa ttgagcagtg gcgtacgttt
    17101 ggtcatccct catcgcttga tcaaaagaga gacagtcgtt cctttagaaa acccatacta
    17161 aatagctgac tcactaaata cgctgaccat ttatcctatg gtcagctttt tatgtaaaag
    17221 aagatactac ttctaacaca ttgtttatct gtaaaaaaga gcgtgggaca aaagtaaaga
    17281 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcagaagca actccttcgg
    17341 aaataagctg aaattcacaa aaatttaaaa agcaattttc ttgagtttct tcttatttct
    17401 tggagttaaa cgcttctgtc ccagcctctt ttttccttcc ttcaattttt tacacgtcga
    17461 cgattcaata aaaaaccgcc taatattgta aagacaggtg caagaatcga gaaaaaggcg
    17521 aatggcaagt aatgcaacgc gctaactttc atcgttccca taatgaatgt gccgctgact
    17581 ccccacggta cgagagaatt gacggtcgca cctgaatccg ctagtgttcg tgtcaaatag
    17641 gtttttgata agccaacgtc atcaaaagaa cgcttgaatg tttctcctgg aagaatgatg
    17701 gacagatact gttctccaac gatcagattg attccgatac agctcaaggc agtaaacccg
    17761 actaaacgag aaggtcggtc catcttttct tttaattctt gaacgatggt atcaacgatc
    17821 agatatttga tcagtaaccc acctaatgcc aatgcaagta aaatcaaact agcagaacct
    17881 aacatactca tgatcccgcc tcgagaaagc agttcatcta gctttggatc aggtgtattt
    17941 gcgacataac cattcatcaa gatgtctgct gcttttccga ccgtcaaact tttctcatgg
    18001 ataaaagaaa gaacgagagc agctgtcgat cccgctagta atgtagggat agccggaatt
    18061 tttttgattg ccccccccaa ataaaataac tagtggaatc aatgtccaag gagagatcca
    18121 aaagctcgct ttcatgtttt ggatcatttc attgatacta gctagatcgg ctgaatgact
    18181 gttatgtcct aatatcgtat aaaaaatcaa cgaaaggacg aatgctggga aatccgtata
    18241 acgcatacca gcgatatgtt cgaatagatt aactcctcca atacccgctg ccaagttggt
    18301 cgtatctgat aaaggcgagc agttattccc taaaaaggca ccggaaacga ctgcgcctgt
    18361 agtcattgca tcattgaatc caagtaaatg accgatcccc aaaaaagcaa ttccaatcgt
    18421 cgaaatcgtt gtaaatgaac tgccgacagt cactccgaca attgcacaaa caagaaatgc
    18481 tgtcggtaaa aagaaacgca cagaaacaag tttaaatcca taaaccataa tcgtagggat
    18541 ggttccagat aaaatccatg aagcaactaa gacaccaatc aaaagaaaaa tcaggattgg
    18601 aatgatccct ggagtgattc ctttgatgat cccgtcatgg atctcatccc atgtgaactt
    18661 tttaaatctg ccataaaaca acaaacacat gaaagctgca aggatcggga tatgtggcga
    18721 gagctggaat ccgatgatca gtactcctag gagtgccaac aacacgatca atacagcgat
    18781 actttctttt aaagaaatat ttttattcat cgtcgatctc ctatttcaaa tgaaaaggcc
    18841 ctgccatgaa aaaactattc acttggcagg acctcctact aaatcagcga caaaacgtcg
    18901 gctgaaagac ttattttttt atttttcttc taaacttttt tctacctcaa tcccccaaga
    18961 atctttcttg aagtgattga tgatcaatgg aataacgaac atgaccactg tgatagctgc
    19021 tactaataat gcatcactca atgtcgctgt acctaatgta gcaaatacga caacgatgat
    19081 agagaagatc agcatgcagc tgactaccca agctaatccg tttccgtttt taccaattcg
    19141 gtaaggacgt tcgaattctg gatgtttctt acgcatttta agcaatgaaa cagcaatcaa
    19201 aatgtacacg atacaataaa taacagttgt agcatttgta agtgtcaaga atacagcact
    19261 tacgtcatcc attacaccat aaagcaaagc aaataatgaa ataacgattg attgtgttag
    19321 aacgacattt ctagatacgc cgtatttgtt ttctttatgg aatccgaatt ttggtggaag
    19381 gaatccttca cgagctactt ggatgattgt ttttgatggt ccagtaaccc aagctgataa
    19441 ttgtaaaagc acaccaataa ataccatgaa gctgaagata tttccgatca cttctggtaa
    19501 gcctaggatt tgacagtcaa tcaagatagg ctgcgtgata tttgctaatt ccatttttcc
    19561 atctggtacg acatttgcta caagcatcgc attgatgaca ttcaataaaa ctaatccgat
    19621 caaagcgata aatacgcctt ttgtataatt ttttgttgcg tctttcaaac gaggcatgta
    19681 aacagaactc atttcgatcc caacaaaaat aaatgtgata ccagctaagt atttcaatga
    19741 atcaatatgt tccaagtcag ggaaagcttt tgaccatgaa aagtcaccta gatagccgtt
    19801 tgagactagt ccgactttga aggctgccaa aacacctaaa acgaacatga tgactactgg
    19861 gatatatacc cctagccata caccgatgtt cccgccgact tttgccatat cgaatttcaa
    19921 gttcaagatc gtgatgatcc agtagataac taaaatacaa cctaaaacga accagtgatt
    19981 attgccaagt tctacattac caaatgtgtt tcctaacaat ggtcctaaag tagaagcgac
    20041 cattaccata cctgggaaca tttgcaccca taatagccat gcaacgacaa atccccattt
    20101 gctgcccagt gctgttttta cccataactg aggaccgcct tcttgaggaa gcatcgtgga
    20161 aagctcaccg gaaaccattg atatcggacc ggcaaaaaac aaaactgcaa aaattgaata
    20221 aaaaatcaat gtccaaccga ctgcggctaa cgtaggaata ctccgaaccg ttgcacacag
    20281 tgccattgtc agaccgataa aggtcccaag acccatttta cttacttcag atttactcac
    20341 cgttgacact ctccttgatt ttttggataa aataacggtg tctttgaagt agtttcagca
    20401 ataaacgaaa caattcagcg aatatttgag gggattcttt gagtttattg cttgaagggt
    20461 ttcttcaaag acaacctcaa aacaaaggga tcgaagagct taggtctccc cttgctcttc
    20521 agatccaaac atgcgtcaga aacagttagt attcaagctg ttttctaaca tgattacctt
    20581 gcttattttg cttcgcttgc caataattgg tctgcgtaga ttttttccaa tttttcttga
    20641 agagctgctt tgattttttc agcatattct tcaaagtgtt cttctttgtt catgtatggt
    20701 gtcattacgc tggcacgcaa tacagttact tttccggcac ggttccattc ttcatctgag
    20761 aatcctagtt ggttcacaaa ttgtaatggg ctgttgccat agtctgggat agcaaagtca
    20821 gtatgtgaag ttaagaactc attaccgtaa atgcttcctt tcacgtatga agaataatca
    20881 tatacgtcat ggttcaattt attcattgca actaagtcat cgttgccttt ttctttgaat
    20941 acatagtcta ccatattgaa atctggataa gttaatggat gaacttcgat ttctttgtct
    21001 cctactttaa atgacaagtc attcaagaag ttgtagaaac gatgtgcacc ttcgattgat
    21061 gcacccatta atttaccata tcctgtgaca ttcaaaggta agacatggtg tgctgcccaa
    21121 acgctggctg ctgttgcccc tgcttttgaa ccttccaaga tataagcccc aagtaatgct
    21181 gggatatctg cgcctttttc aaatacatat gtcgcaaagt aagagataac gtctctcatg
    21241 cggatatctt tgatgacgat accaccggca gaatatggaa catagcccat tttatgtggg
    21301 tcaatcgtta cagattctgc ttcttcgatt gctttatatg cactgtgtac ttcttctaag
    21361 atataatctt tgttttctgt aaagacattg tatttgtaat gaacatcttt taaatcttca
    21421 aatggaatga agttgttgtc ttcgtctaag aaaatcgcac ggccgtatcc gccataagca
    21481 gcatctacgt gtaagtagaa gtaaataccg tctttttcta atacgcgacg caatgcaacg
    21541 attttatcga taccgtcgat tgctccttct tctgttgatc ctacaacacc gactacacct
    21601 aggattggtg ttttttcagc tgctagacca cgaacgattt tttcaagttc gttgatgtcc
    21661 atgcgatagt tgtggtcaac tggcactgga atcacttggt ctaaaccgat accgatgatg
    21721 tcggcagctt ttagccatga atagtgttta gtttgtggaa ccaaccattt tcctaatttt
    21781 tctaaatgtt ttccgctacg tgctgagtga gctttgattt cgtcgatttt ttctgggacg
    21841 ctgtctaaca gattcatgat ttcttctgtt gacaagttca ttaattccca atcgcttttt
    21901 ccagcaacta gttcaggtgt cacttctttc attgcaagcg gcaatgattt gatgttgcgg
    21961 gcataccaaa gtccttctaa gtttgctaaa gaaccatcag caacgatgtg gccccagcca
    22021 tctttatagc tcattaattt tgcaaattcc attcctactt cttcttccat ttggcttgtt
    22081 gcaggagaag attcataggc aacgttatta ccattccata gcatagcaaa attgtacgct
    22141 agtaaagatg gcatcaatgt ttcagagttc atatgacccc agtaacgtcc ggcattatgc
    22201 caaggaacgg aatgggtacg catccgcgct gaaatttctg atagaacatc ctttgtttta
    22261 ttcacagtgt gttcaaaact agcactgctt ttttcttctg gcgtaataat aggcatgtct
    22321 tgaggcatgt agttttgacg ccagcctaaa tgttcatcca ccaattcatt caacaaagct
    22381 ttatagattt ggccgttctc agctttgtcc ccaatgaata gggcatttag gtttagatct
    22441 ttcgacaatg attcactcat agtgaaaccc tcctaaaaaa atatttaaat tttgtcgagc
    22501 cctacattac acctgttctt ctattctgta aactaagttt tggcctgatt tttaatcata
    22561 ttttaatatg tatcaattat ccgaattgtt cacacttttg aaataattaa aaaaaacgag
    22621 ggaatttaca aatgtaaact ccctcgcaaa gattgtatta acgacaacca gcgttctatt
    22681 ttaggatggg tttcaaaatt ggaatgttgt aatagtattt ccctttttca aggtaaaaac
    22741 ttactaaatt ctaatcaatt actttggcca aaaagtaatt tttcttacct tttcgaataa
    22801 ccacaaactt accatcgaat tcatcggatg gatttatgac aaaatttaaa tcggtaacgc
    22861 ggtcaccgtt aatacttata gctccgtttg aaacatcttc acgtgcttga cgtttagatg
    22921 gttcaatttt tgtggaaaca agcagttcca cgatattttc aggtgtgctt gaaatttcaa
    22981 cgttcggcat tttaccgaat ccttgcgcga tttcttccgc atttagttct ttaatatttc
    23041 cagagaacaa tgcttgcgtt attttttgcg cttctttcaa gtcttcttca ctatgcacaa
    23101 atcttgtaac ttcttctgcc aaacggcgtt gtgcttcacg tttctcaggt tctgtctcta
    23161 cttttttcgc taagtcttcg atttcttctt gagacaagaa agtaaagaat ttcaagtatt
    23221 tcactacatc gcgatcatct tgattcaacc agaattggta gaactcaaat ggtgaagttt
    23281 tcttcggatc aagccaaacg gcaccgcctg cagtttttcc gaatttcgtt ccgtcagcct
    23341 tcagcatcaa tgggatcgtt aaaccgaacg cttctgcttc tggtccttct aattttcgga
    23401 ttaggtctaa tccagctgtg atatttcccc attggtctgc tccaccgatt tgcaattgca
    23461 catcgtatgt tttatgcaga tgcaaaaagt cgatagattg caggatttgg taagtaaact
    23521 ctgtaaatga gattcctact tccaaacggc tggcaacgat atcttttgct aacatcgtgt
    23581 tgatgttgaa gtttttaccg tagtctctca aaaattcaag taaagagatt tttgacaacc
    23641 agtcgtagtt gttcacaaat gtgatatttg catctttacc aaacaatttt ctcatttgat
    23701 tggaaagtgc gtccacgtta tgttgcactt gttccatcgt ttgcaacaca cgttctgttt
    23761 tgcggccgct tgggtcgccg atcgttcctg ttccgccgcc aatcagaata tatggatgat
    23821 ggcctgccaa ttggaatctt ttcatcatca taaatggaat caaatgtccg atatgcatcg
    23881 aatcaccagt tggatcaacg ccgcaataaa gtgaaatgct cttttcttca acgagctcgc
    23941 gaagtctttc ttcgtttgtt tgctgattaa tggcgtcgcg ccaagttaat tcatcaataa
    24001 tattcatttt actgacctcc gtgtagtttt tttatgaaag caggaacctg ctagcatgtg
    24061 taattaatta tttcttcagg ataggatagg aaataaaaaa agtcccttgg catataagcc
    24121 aagggacgaa atctcgcggt accacccaat ttgtatgtct agatatccaa catacctctc
    24181 gaacatttaa cgctgtcgcg tggcaatcac tgccaaagac tcctgaaatg taattcgctt
    24241 taaagcatgt actggtttgc agcgaccacc agctttctga aacattggct ttaacaacta
    24301 cttcgtttcg atcttcgtct tgttattatt aatatgtgtc tatcatagag cgcaactttt
    24361 tctttgtcaa cagaaaaagg atattttttt taaattcctc attttttctt ttttgaatcc
    24421 gctctaattc cgaacatttt attttcaatt attgttaaaa gaaataactt tttctgtatt
    24481 ttttctgcaa tttatcgaga agtcacttta ctttgcctct tattctttct tcgttacaat
    24541 aaggaatggg acaagtaaaa aaatgaaaaa gcaagggacg aggtatatgg aaaaaaagca
    24601 aaaaattatt ttaggagcca cttctgcttt aactatcgtt attttaattc taagtttgtt
    24661 ttttgtcatc cgatcatcta caaaaaaaga accaatcatt cctgaaaaaa aaataacaga
    24721 aaaagcaaaa gaaccgacag atgataagca acaactagtt actcaagcaa aaaatctggc
    24781 tcaaagctac gattatgacc aagcgattgc gttattggaa aaagatagta agaacggatc
    24841 tactgaaaca caacagcttc ttcaaacact aaaaaaggag aaagaacaac tgatcgactg
    24901 gtctgatcct actcagatct ctcatgtctt tttccacagc ttgatcgtgg acccgcaaaa
    24961 agccttccac tctcaacagg cacaaggata caaagattat atggtgactg ttgaagaatt
    25021 caaccgttcg atcgaacaac tttatcaaaa tgattttgtt ttagttaatt tgaatgattt
    25081 gatccaaaaa gacgaacaag gaaatctttc ctttaacggg cttgcgctac ctgaaggaaa
    25141 aaaaccattg gttctttcac aagatgatgt cagctattat gaatatatgg ataattccgg
    25201 ttttcctagc cgattggtcg tatcaaataa caatgaaatc aaaaatgtct ataaagagaa
    25261 agataaagaa gaaatcggtg actttgacat ggttcctctg atcgatgcct ttgtcaagaa
    25321 acatcctgat ttttcttatc gaggcgcaaa aggtaccttg gccctgacag gttacaacgg
    25381 agttttaggt tatcgaacat caaaatctca atatggagac aatgaaaaaa caaaaaaaga
    25441 gattaaagaa gctaaaaaag tggcggaaca gttgaagaaa gacggctgga gctttgcttc
    25501 ccatacatgg ggtcacttga atatgactca agcttcacta gaagatgtaa aaaaagacaa
    25561 tgaattgtgg cagcaagaag ttgcacctat cctaggaaaa acaaatattc tgatttatcc
    25621 atttggtgca gacatcagcg actggcagcc ttattcagca gaaaatcaaa aatttgctta
    25681 cttgaagagt caaggtttcg atattttctg taatgtcgat gcttctacac cagcttgggg
    25741 acaattaggt acggatttct atcgaaatgc tcggatcaat atcgatggca ttcgttttca
    25801 atctgattta gacggaaaaa atcctattct tgatccattc atcaatgtcc atgatgtcta
    25861 tgacaaagaa gcacgcgaat ctgcttgatt ttacgtcatt tatgaaccaa aaaagccgaa
    25921 ctgacatttt atcgtcagtt tcggcttttt tatgctttat ttcttcttcg gtgcgattcc
    25981 cttgatcagg acttctgtcc attctgctgt atatggaagc agttttgcgt atatctccgg
    26041 cttagaaatt ccttctacca ttgcttgaaa atagaggatc aagaactcat ccgaatattt
    26101 caaatccact tttccttctt tccgccctcg atggaaaaga tcaagcatga tactgaaact
    26161 ttcttgcgta tacgcatcca tcgcgtcaga aaattctttt ttttcttttt ttgtatagta
    26221 tgtcatcaaa tcgatataaa attgtttgtt cactcgattg agataacgga ttttgttttc
    26281 actcatggca atcagtgttt cttcaaatgg aagattttcc gcaatcaacg ctttcgcttc
    26341 ttgccctaaa tcattcataa atgcaaaaaa tacttcataa atcagccctt ccttgctgcc
    26401 aaaatattta aaaatcgtcg ctttacttac ctgagcgttc tcagctacct tatccattgt
    26461 cactccagcg gttcctatgt ctgtgttcat caagtcaaaa gctgcttgta aaatctcttt
    26521 ctttttctct tgtgctctct tttcaaaacc gttcatttgt agagcctccc gtaattttaa
    26581 acttttataa tatatatagt tcaaatttat tctgaggttt attataacta tctattgaga
    26641 aagtatcaag ctagcaattc cttgtttatg aattcattga cagaaattta taaaaagaat
    26701 attatgtact aaaataataa aaaaagtttt ataaaaaggt ataaaacctt tttattaaac
    26761 tatttaatct tgtttagtat atatttttag aaaaaggagt ggggttttta tggcagaaat
    26821 cgtcaaagtc acacatttga aaaagagctt tggtcgtttc gaagctttaa aagatgtctc
    26881 tttcaccgta cattcaggtg aagtcgttgg atttatcgga ccaaatggtt ctggaaaatc
    26941 cacgacgatt cggacacttt tagggattat aaaaaaagac ggcggaacgg caacgatttt
    27001 tgggcaagat gtatgggaag atagtttatc tatccatgaa cgtctttctt atgttccagg
    27061 cgatatctct ttgtggggaa atttgactgg aggagcgatc atcgatctct tcatgaagct
    27121 tcatggaaaa gggagtgctc aaaaaaaaga tgaactgatt caacgtttct ctcttgatcc
    27181 taagaaaaaa gcaaaaaact attcaaaagg aaatcgccaa aaagtgggct tgattgctgc
    27241 tttggctgtt gattcggatc tctatttatt tgatgagcct acttctggtt tagatccttt
    27301 gatggaaacg attttccaag aagaagttga aaaattgaaa gaacagggca aatccatcct
    27361 actttcttct catattctca gtgaagtcga gcgactagcg gataaagttg tgatcatacg
    27421 cgaaggaaca ctcgatgaag aaggaacact cgatgaattg cgtcatttga ctcgttcgac
    27481 catcactata gaaacagaag cagagaatag tcacttagca caattgaacg gtgtccatga
    27541 ctttgtgcaa aaaggtcacc tcgctcaatt tgctgctgat aacgaagctt tcgatgtgat
    27601 tttagctgaa gcgtccaaat taggtgtgaa aaaatttgaa tctattccac cgactcttga
    27661 ggatttattc atgagacatt atgaaggatg aggaggaagc gaaatgactg ataaattttc
    27721 acgctggtcc accctttttt ctcaaaacct tagccgtgac tggaaaaaga tcctgatatg
    27781 gctagtcggt gtcactgctt tttctggtgg ttttgtccct gcgtttgaag aattgacttc
    27841 cggacaagga ttagctggat tatacgaaac cttgcaaaac ccggcaatga ttgcaatggt
    27901 cggaccaact ccagcttcga atgctgctga gtatacttta ggagcgatgt atgcaaatga
    27961 aatgttgctt ttttgcagtt tgtttgctgc tgtgatctct gttttacacg tggtgagcca
    28021 aacgagaaaa aaagaagaac ttggtctgat tgaacttatt cgttcctttc gagtcggacg
    28081 tcaagcaaac tctttagctg tcatcgctga aagtattctg atcaatttgc ttttagcaat
    28141 atcgatcagt tgtacgatga tttttttcca agcggattcg atcactgcag aaggtgcttg
    28201 gctttttggg ctatctgtag gtagtatggg gattttgggg agtgccgttg cactgtttgc
    28261 tgcacaactg atgccaagtt cagccagttc gaatggtctg tctttagcgt ttttaggtgg
    28321 actttacctt ctacgagcaa taacagatgt agctgattcc tccctttcga tgtggaatcc
    28381 tttaggctgg tcttatttga cctatccatt tacaactaac aactggattc ctttgcttta
    28441 tttgtttttg ttcagtctaa tcttgataat cagttcattt gttttagagg aacatcgaga
    28501 tttgaacacc ggctatatcc ccgaacgtga aggaaaaccg tatgctaaac gttctctttt
    28561 atccttgcgt ggtttattat tctatctaaa taaagggatg acaatcagtt ggcttatcac
    28621 ttttggtttg atgggggcgg catacggctc tatctacggg aatatgcaac agtttttaga
    28681 aagtaacgac ttaatgaaac agatgtttat ccaatctggc gtctcaattg aaaaaaactt
    28741 tacaagcacg atcatggtcg tcttgatttg tctagctgct attttaccaa ttgctagcat
    28801 caatcgttta tttacagaag aaacccacgg acgtatgaat cagctgttcg caacaaaaat
    28861 ttctaggaac caactttatt ggaccaatat gttactagct attctatttg ctatattggc
    28921 aatcctgatt agttctggtg cactagggct ttctgctttg gcttcgatga aggatgcgaa
    28981 agacatggat ctattgatgt ttcttaaagc cgggttcaat ttcctgcctg ccgttttagt
    29041 gtttatcgga attagtggct ttctattcgg atggcttcct cgaatcggta aacttgctta
    29101 tatctactta gcttactcct ttgcactcaa ttactttggc ggaattttgg atttaccaga
    29161 atggttttcc aaaacagctg tccctagctg ggttccacgg atgccaaacg attcttttga
    29221 tctttccgtg ttccttacgt tatcatgtat cagccttgca ctgttcgtcc ttggttctat
    29281 tggttatagg cgaagagact tactagaagg ataataaaaa agcatgtaca atttcatgct
    29341 agcctccaaa tctgggtcaa aaatccaaca tttggaggtc atttttttat ttacaagtct
    29401 tctaactgct ctttatcaat gatccagact tctcgattct ttctttttac ataaccattc
    29461 tcctctaata atttaaattt tctagataaa gtttcggggg tcgtaccgat aaatgcagca
    29521 agttctttca ttttcattgg caggaaaaag tgatccttcc ctgtcgcttt agctaagtca
    29581 agcagataga cagcgactcg ttcttctact ttttccatca tcaaaaaagt tgtctgctgc
    29641 tgtacttttg ttaatttctc aacgttgatt gctaaaagtt tcagactaag ggcgggataa
    29701 tgcagtaaga gttccgagaa atctttttgt ctcaatatgc aaatttccgt tttttctagt
    29761 gcttcgccaa ataaagactc atttggaacg cctagcaact ggctctctcc ctcatatccg
    29821 cccggctcta ttactcgtaa aagctgttcc tttccgctag cagataattg atacactttc
    29881 atactgccgg aggccacgat cactaattga ggttctccat tcggcgcaag aatctgttct
    29941 cctttttcca catagtgatg tttgacaagt gcgtgtactt tttcttgatc ttgctgtact
    30001 aagtgattga acaaaggaac aaatgaaaca cataaatgat catgactcat aatctctcct
    30061 ccttctaaac agctatttct aataaagaaa cataacttcc cttattccaa ctgataattt
    30121 tcttttatca tagcataaac gtcaagcgaa ccagatacca gcaaatagag tggatttctt
    30181 tttgtccctt gctttatttt ccttcttttg agaccttaag taattgattc ctatcaattt
    30241 tttaaatatt tactttttga ataatcaaaa acaaaccttc actcgcaagc cgagagtaag
    30301 gtttgttttt cgcattgctt atttcgtgtt gtctattgaa atttttccgc acctagcttt
    30361 tgatatagtg gtgctagttc ttgtaaggtt tcagcggtta tttgctcaac gtgttcgatt
    30421 tcttgcggat agaagaatag tttacccatt tgaaaggttc ggtctctcat cgcttttttc
    30481 aactcttctt gtttctttgt atcggacaaa tagtcagaca aagctaagtg gtcgtcaagt
    30541 tcatgttctg gttgcggcca tatgtaataa tccttgatat cgactgtttt gctccactct
    30601 ttcagtatcg caagcaactg gttatacgct gcaatgctac catatcgttc ttcgctttta
    30661 taatgttggt acatcaaata gatttggtat tgtttttgat tcaacaaaac tgctagacag
    30721 gcattctcgt cttgacgaga ttcgcttcgg tacgcgctcc aaaaatgact gcgtaaattc
    30781 cagccattcg tccagctttc gattttaggt ttgctcatgc cgtacatatc aggcaaaagt
    30841 gcagctgtct ggagctgaat ctctttccat ttttgccagt gttctttata ttctttcttg
    30901 atttgttcaa tcttatctgt tggatggctc tctttgagct gtttgaatgc gtagattggt
    30961 cgatcaaaca actgataatc tgattttcca agttccattg tttcgcctct ttccgttatc
    31021 ttttgtattc tacctgaatt ttgcgatatg gacaaataga ggttgtgaaa aagctgttct
    31081 gcatcaagtc ataagaacag ccaaacaaaa aactgcgatg agcatcaccg cagttttatt
    31141 gaacttaagg ttgattgatc acatctaaaa gcgttgaaaa taaaatggca ctagaagcag
    31201 ctcctgggtc gatatgtccg attgcacgct ctcctagata ggaagctcgt ccttttttag
    31261 ctaccaagac tttggtgtgt tcttttgcct ggtcaatgat ttcttttgtt aaattccctg
    31321 ccttcaatgc ctctgtgact ggcagccaaa catctaacat cgttttatcg tcagcttctg
    31381 cctttcctcg gctttgaata cccgctgttc cctgctcgat cacttcgcct aatttttctt
    31441 gagaatcaat agaatcgacc tcttttgtcg ctttcatcat gttcatgaat gcagatccgt
    31501 ataacggacc agaagcccca ccgactttcg atatcatcgc catagagaat actttgaatg
    31561 tatcgctgat tgtttcagga tgatcggctt gaaaagcttc ttcataagct gaaacccctc
    31621 ttcccatgtt atttccatgg tctccatcgc caatcggggt atctagatca ctgagatatt
    31681 gtttgttctg attgattgcc tctgcaaatt gcgtgagcca ttcttggatt tcttgaacgg
    31741 ttagtttcat attatcaccc ttttatcttg agatttttta ccatgaaatc gtctgtacat
    31801 tgctttctaa agaatctttc cattgtgtct ccgtcaagtc gatcagtgtt agagaaagac
    31861 cttgcatatc tagtgaggtc ataaaattac cgacttttcg gaaaactaca ttgacgcctc
    31921 tttcttccaa cagagctaaa acatcattca tgaacacgaa ctgttccatc aaaggcgtcc
    31981 cgcccattcc gttgactagt acacctgctt cttttggtaa attcgagtaa tcatctaaaa
    32041 tcttagtaac aagttccttt gccagtactt tagaaggctg catcttttct cgacgatagc
    32101 ctggctcacc gtggatacca acaccatact cgatctcatc ttctcctagt tcgaatcctg
    32161 gtttccctac ttctggtaca gttgctgctc gaagcgctag accgatcgtc ttagttgcct
    32221 gtacgacctt ttctcccagt tctttcaact cagctaggga agcaccgttt cttgctgcat
    32281 cgcccacgat tttgtgtaca aggacagtac ctgcaactcc gcgttttcca gcagtatacg
    32341 tactattttc aacggcgata tcatcatcta cgatcacact ttctacttca atatcgtcca
    32401 tagcagctaa ttcctttgcc atatcgaagt tcaatgcatc ccctgtataa ttcttaacga
    32461 tcagcaaaac tccttttcca ctatcggctt ccttaatagc cgtctgaatc tgatctggag
    32521 ttggagaagt aaacacatca ccaagcaccg ctgcacttaa catcccttcc cctacaaacc
    32581 cggcatggga tggttcatgt ccgcttcctc caccagaaac caaaccaact tgttccgctt
    32641 tctgattctt ggctacaaca cgggaagatt ctacacgatg aacaagttct ggatagcttt
    32701 ttactaatcc ttccagcatt tcttcgacaa tattgcttgg atcattcatg atttttttca
    32761 atttattccc atcctttctt tccggcattt tgtgcttctg ttaagatttg ctgagtatca
    32821 tctgttatac cggcagtgat ggctgctgca aaagctcctt ccactaaagg cgcatccacc
    32881 agatgataac gttgctgctg ctcttcttcc aacatgtctt ttgccagttc cgagttcaat
    32941 accgcgctac cgatatccgc aaaaacaaaa taactagctg catcgcttga ttcattgact
    33001 gcttctacga ttttcatcgg gtctgatccg atctcgccat ttattccgcc gagagaaaaa
    33061 atttgcacct cttcggattt ttgcatctgc tcaatcattt ctttaatacc atctgttact
    33121 ttttgactat gagaaataag caaaatactt tttttcacaa gaatcttcct ttcttttaca
    33181 tagttgctaa cgattggttt cgctttcatt aatgaacaat tcaagtatat aataaatttc
    33241 taattttggg aaaatatgtg tagtgcttct tgaagtttgt tatactttaa ttaaaagaga
    33301 ggagtgatcg aatgattaca acaacaacga acagtgtaga gggaaaagcc gtagtagctt
    33361 ataaagggat cgtttttggt gaggtcatta ctgggatcca tgcatttaaa gacttggagg
    33421 cggggttgcg aaatatcttt ggcggccgct caaaaagcta tgaaaacgaa ctaatgggtg
    33481 caagagaaga agcacttgcc gaaatggccg aacgtgcaaa agcattagga gctgatgcga
    33541 tcattggcgt aaaaatggac tatgaagtat taggcagcga caatggcatg ctgatggtca
    33601 catgtagtgg aacagctgtc tccacgagat aagtagggga catctgtttt aataactaaa
    33661 gagagatggg acatgtagtg gcactcaaga attagatttt tcagtctaac ttcgaggtac
    33721 tctaccttcc catcctctct tttttatttc tttttgcgga tgattccgta agctgctgcg
    33781 actaacacac ccgcccctgc taaggaataa ctgacagaag ctgttttttc atttgttttt
    33841 ggcaaagtct gacttgttga tttggatggc tctgtcgttt gctgaactgt ttctgtcgtg
    33901 cttccagagg tcactgattt ttctcctgcc gaatgcaaaa tatcttttat tcgttggaaa
    33961 ctagctgcta agtcagcttt ttttgcttca aaatcattag caactgtttg tgcttcgctg
    34021 atctttccgt ggattgcttg actggccgct gtgatatccg cttgagtttg acttagcttt
    34081 ccagaagctg tttgagcaac ctgatttaat tgctgattga attcttttgc ttgttggatc
    34141 ttcccgtcaa gatctgtttg gacagcttcc gcttttgctt tcgcttcggc aagctttttc
    34201 tcaagttgtg cacggtcatt tcccacttta tttttaagat cagctaaatc ttgatcgatt
    34261 gctgcttggt cttctttcaa tttgtttttt gcagcttcta atgcttcttc gagttctttt
    34321 cttttatttt ccaagtcttg caaatgatcg atttggtcag aagaggtaga actatttatt
    34381 gatgtatctt ctgaagatga aatcgtttct cctgaattat ctgtcggtac attggaagtc
    34441 atttctgttt ccgaggcacc gttttcagca ccagaagagc ttatggttgt tgaaggttct
    34501 gtttgagcag gttcagaagt ttccgcaaaa gctgtctgtc ccaataagaa aatactggaa
    34561 acagcaattg gccagattaa cggcttcatt ctttttttca tcacttttcc catccttact
    34621 actttttaac taatttctta ttataagcaa aaatgtttgg aagaaccata aacacaacag
    34681 gctgtttgtc taaaaaacgg tgcatctttt atttttttgt acgttccggg cttttccatg
    34741 tcccgcttcc ataacctaaa atcgttttga ctgctggaat gaaagtcgtc acgatcgtta
    34801 tcgcgttcac tatccagaac aaaagcatat atagtggtgc gaaaagaagg tatttcaatt
    34861 ttcttcgtcg atcatctaca atcagcgaag ctagcaactg gaaaaatcct gcgaccattt
    34921 caaagcagat gaacacaaaa ctgaatgtaa aagtaatata aatctgttcg tagtttcctt
    34981 gatatacata atgcccgatt aatccagcaa aaagaactac ggaaatacaa aagaatattg
    35041 accagacaat actcaatgtt tgatcaatga agatgatcgt tcttccaata tgtttaaaag
    35101 gatggagcat cacttttttg aaattcgtca gccagacttc tgttccacct tttgcccaac
    35161 gttttctgtg tcgatatagc atcttcaacg tcacaggtac ttccatgaaa aagataatct
    35221 caggagcaaa aacagataac cagtcattta actgatgatc ccaagcaata ctgatgtctt
    35281 ctgtcgctcg atcttgtctg aatccgccca catcgatcaa ggcttctttt cgatacatcg
    35341 tatttgctcc actgtacgca taaagaccac cgaaaacagc tgactgtgtt cgtttaatga
    35401 tgcccacgat acttgagaat tcaaccgttt gagattttgc aatcaatttg gtacgatttt
    35461 ggacatccat atttgcagtg accgcagcaa tatgtcttgc accagggcga atgaaataat
    35521 tcacatacct aatcagtgca tctggctccg gtaccgtgtc tgcatcattg cttaaaatca
    35581 atttcccttt tgcaaaggca agtccgatat taaacgcgtg ggcttttcct ttattttttt
    35641 cgatccgaac cacacgaaga ttagcgtatt ttttcatcag tctagctaat atttctggtg
    35701 tttggtccgt tgaaccatcg tctgtcacta atacctcata gttgtgataa ttcaatttcg
    35761 tcatcaaata ctcgattgta tattcgatca caatttcttc attatgggct ggcaccatga
    35821 tcgtgatgaa cggttcgaca ctcaagggaa catctaccca ttctttttgc ttatgtttgt
    35881 ataaaaacac ataacaccag acaccaataa accatacaaa tccgcctaaa atcggataag
    35941 aaacgagaaa aaaatacata atagacaaac aagtatctaa gaataaatta cctgtatggg
    36001 tgattgagat catttcagtt ccaccccatt ttctttatac aaatctgcaa tgaatgtcgt
    36061 atctagattt ttctcttcgg agacagaata aaaacggacc gtttctctct cttcttttgt
    36121 tccaaaacgt tccgtataca cttcttcaag taattcttgt ctttttttca atttttcatc
    36181 ttcatattca aaagatttat ttactttttt gtatagattt cgattgttcc acctagtcaa
    36241 tacaaatgag gtaatcagga aactgaaaaa aatcaataca aaaaagccga ttaagaaata
    36301 cagcattttc ttttctgata aaaaagcgta caaggaaaaa tccttggctc tttctggtct
    36361 cgttaatggg aaaaagaccc atataaaagg aagaacaacg atcatccatc ctaaaagagc
    36421 cactaacgtt tgccaaatct ttaaccagta attacctttt gaaaaatact tatctttcac
    36481 caaaattgat gcaacttctt ctcttgttac ttttttcata tcctcacctc acgttttttt
    36541 gttacgacgt aggagtttac catacatttc atttcttttc acataaagtg ttcctaaatt
    36601 tttcctaaga aaaagaacaa aacatttctc atcagcaaat gaaaaatatt ttgttctttt
    36661 tatcagttgt tctttttatt ttttatctct taattatgtt gctctttcca atacgacaat
    36721 gcttttcatt cctctagata tcgccgtgta catttgtctg cgtcttcttt gctcatctcc
    36781 atcgttttca aatggatata agattacatg atcaaattcc agtccttttg ctagactcaa
    36841 aggaatgatt tggcagtttt ctaatccata tgaatttttt ttaatcgatt cttgtagcgt
    36901 ttgggtttcc tcttctgttt gggtgataac tgccgctttt tctcctttta gagaaaccaa
    36961 aatctcagct agtattttca acaattcttc agaggatcga aaagagcggt attcgggttc
    37021 ttttccttgc ggtcggatcg atacgatgtc gatcttctct tgatccacag cataggtttt
    37081 gaaaagctct gtgattgctc cgctagaacg gtaactattt ctaagatcat accgatggat
    37141 cggcagatgg tagtcgtcga aacaacgcat aatatcagca aaagtagtgg aagaattaaa
    37201 gattgcctga ttctcgtcac ctactaaagt aaagtgagtt tttggaaata attcgatcaa
    37261 tagacctaat tgtgccaggg tataatcttg tacttcatca atcaatagat agcgaaatgc
    37321 tggtacattt accttttcaa caagtaaatg acgaatagct aatataatga ctgcttcatc
    37381 aacagtacgt gtgggttgcc aagcataagc tgagccatat cgtttttcat aaattctttc
    37441 aaacaactga tgctcatcca cccatgccat ttcttcgatt tgtctggtta tttttcgata
    37501 ttttttttgt aaaagtttcc ttgcataagc tacaatactt tgttccgaat catcagaaat
    37561 caatttaccg aataattcca actgcctatc ttctgtcaac gagagtacct gattctgcac
    37621 agcaggactt tttgcttgtt tcaaaagatg attcttccat tcgctcatca gtgccttttt
    37681 cgttgcttga agcctatcga tcaatttagg atgagcagga actttctcat atattttttc
    37741 gatcgttttt ttagaaagga ctgtttttcc atttttagta agtccacgga taaaattcgg
    37801 atgatctaaa aacaaaggat ctgattgttt tagattatcg ataaactttt tgctacgcaa
    37861 acgttctgtc tgctccgata cattttctcc cgtgatacgt ttgaaataag cctcttcacc
    37921 ttctatttcc tctgctgaaa gctgtgaaac cagctgtatg atggttagat tcagtggatt
    37981 tctttcacct aatgatggta ggacattcga aatatagtcg ataaaccgat gattaggtga
    38041 tagaatcagc aagtcatcgg atgtcatcgt atctcgatat aaatagagca gatacgcaat
    38101 acgctgcatg atagcagagg ttttaccact tcctgctacg ccatttacca agatattttg
    38161 acttttcaca tcccgaatga ttgtgttttg ttccgattgg atcgtagcag tgatagcctt
    38221 catttcattg gtctcatctt ggcctaagac ttctaataat acgtcatctt gaatagctac
    38281 agacgtatca aaaaacttga ccaaacgatc tctttccaaa agaaattgtc gtctttcttt
    38341 aataaccgta tcgattttat tttgacgaac cgtataagaa gatggaccaa tcatgttatt
    38401 gtaaaaaagt tcggcaattg gtgaacgcca atcataaaca agattttcat ctgcatcatc
    38461 tgtgaaattt gctgtaccga tataaaagga ttcttttggc tcattatcca gaaagtctac
    38521 aacgactttt ccaaagtagg gacttttcaa taaacgttcg atctttgcta gctgattttc
    38581 tgcgtttttc tgtttgatat tcatctgatc gatttctcta tttttcattt caatcatcga
    38641 aaaagtatcc aggttatcta agtaactgtc aaaattcaat cgaagctcgc cagccatttc
    38701 tttcaacgac tggtttcctt ctatttttgc cgtatccatc gattcccgca gttcgttttt
    38761 tttatcaatt agcttttgat acacgttctt cagatatgct tcttctaact gtttttgtgt
    38821 gttgaccata cacattctcc tcttttttta aagtggtgga accgattagt ttacctcttt
    38881 taagagtcga atgcaagctt tttaacgagt atcatttgtt aggatatatc agttcttcag
    38941 gtcggacagt ttgatataaa gaaaatgcac catcaaggtt ttttacgtgg tagccagact
    39001 gcttcaagat tctttccgct agatagctac gtaatccgct atgacagctg acgatatatt
    39061 cttgttggct gtctaattca tttaatcggt cccgaagttc attcaaaggg atatgtttgg
    39121 cttccttgaa tttgcccttt tccaattctt ctgctgttcg cacatctaat aaaatttttc
    39181 cttttgctag ttctgtcggc aattcatgcc attgtacggt ttcactaagc ccttctgcaa
    39241 tgttcattgc tgcatatcct agcatgttta ctggatcttt ggctgagcca aatggcggtg
    39301 catacgtcaa ttctaattcc ggtaaatcga agatcgtcaa atgccctttg attgctgttg
    39361 ctaaaatatc gatccgttta tccacacctt ttgccccaac accttgtgca ccataaatct
    39421 cgccagtctc tggatggaag atcaacttca gtaaaatatc tgttgcttct ggataatagc
    39481 ttgcatgatc ttttcctgat acatgaatga cttcaaaaga cagttcgttt tctttggctg
    39541 ttctttcact taaaccggtg gatgcggcag tcattccaaa cgttcgtata attgctgttc
    39601 caataccgcc ttgatttctt cgagaaattc cggaaatcgt gtccgcaact tgtctacctt
    39661 gacgattggc tggtgaagca agagaaataa gtgcatcttt tccagtgatt tgctgtttta
    39721 caacgatagc atcccccacc gcataaatat caggaatatt cgtttgataa cgttcgtcta
    39781 ctaggatacc cccgcgtatg cctaattcga ttccagcggt cactgctagc tggttatcag
    39841 gacgaacacc aatggataaa ataactaaat cagcagggat ggtacgccca tcatccagtc
    39901 gcaatcgttt cccgtggtct tcaaatccag cgacagcatg agaagtaatc acttctactc
    39961 cttgatgaga caattctttt tctataaaag ctgccatttc ttcatccagt gttggaagaa
    40021 tatgtggtgc ttgttcgatc accatgactt ctaaacctct tctttttagg ttttctgcca
    40081 tttccaatcc gatgaatcct gcaccaacga tcacggcacg ctttggctgt ttttctaatg
    40141 catgcatcac tttatcgata tctggtacat ttctaataga aaaaacattg tcggcttctg
    40201 ttatccctgg aatcgatgga acaactggtt ttgcaccagg agataaaacc aatgcatcgt
    40261 actgttcaat gtgtttttga ccctcatgta cgacttctac cgttttgttt tcgggaaaga
    40321 tggctgtcac ttcatgattt ggacgcacat ctaaacaaaa acgagcggat aatgattctg
    40381 gcgtttggac aagaagattt tcccgttcac tgatttctcc cgaaagataa taaggcaagc
    40441 cgcaatttgc aaaagaaaca tacggtcctt tttcaaaaac aacgatttct gcatcttcca
    40501 ttaatcggcg aagccgtgta gcagcagaca ttccacctgc tacacctccg acaataacga
    40561 ttttcatctt atttccctcc tactgttcga tcaaaccatt gattcatgcc accacgtaca
    40621 ttcactacat cataaccaga tttctttaat tctttagctg cttgtttact cctcatccct
    40681 gattgacaaa tcacatagac ggtcttttct ttgtctccat catatctgtt tatactttgt
    40741 aatggaacat ttttagctcc ttttatatgc ccgccccggt attctgcagg tgttctaaca
    40801 tcaagtaacg ctggattttc caaaagcttt tcctttaatt ctgggactgt tatcgatttt
    40861 attgattgaa atagcaaatt cattttctgc acctcattta catttttata aatacctcat
    40921 ggggtatatt aaacaggagc tttattaata tgtcaaccag aatgactctt catttttttc
    40981 actacttttt atatttgcat aaaataccga taaggggtat tatctaatta taaaaataga
    41041 ggagaatgtg ttatggagat tgataataaa aatatcctga accgtttgaa acgtacagag
    41101 ggacaaattc gtggtatcca aaaaatgatc gaagacgaaa aagaatgtat ggatgtcatc
    41161 actcaattaa gcgctgttcg ttctagtatt gatcgcgtaa tgggcatgat cgttgcagat
    41221 aatctgaaaa attgctttga gaaacctgat agtaatccag aagaacaagc ggctaaacta
    41281 gaacaagcga tcaagatgat tattaaaaag taaaggaatg atttcaatga aagtcgtttt
    41341 ccatattgat gaattagaaa aatggtcaga aacagggaaa aacgtaaaaa acctgataaa
    41401 agcttctcca gaaacgacga ttgtcgtatc tgtaaatggc atcgcgatta ccggttatct
    41461 agactctgcc aatgcagaat tcttagattt gcaaggagtc gtttttcacg cttgcgccaa
    41521 tgctatgcgt gctaatcata tttcagaaag ttctttacct gaacaagtaa tcgttgtacc
    41581 agcaggtgtc cttgatttag ttgaactgca atcccaaggt tatgcttata tcaagcctta
    41641 gtcagtagag gttgcgacag aagtgatcag cttcaagaaa tttcggctta tttttcgaag
    41701 aagctacttc tggaaaaccg tgaatcaaag gttgtgaaaa cgctgtcctg catcaagtaa
    41761 taagaacagc caaacaaaaa tagcttctca tatttttcgt ttggttgggc ttattaccgc
    41821 agatgcaagc ttttgaacac cgtttaagga agaggttgtg aaatcagcgg tttgacctaa
    41881 gtattagaga aaaaaacggg aaaatagctc ctcatatttt gccgtttttt gagctaatac
    41941 cgaaggtcag ctgattgaac accgtttaag gaagagattg tgaaaaagtc gcttagtttc
    42001 gagtattaag aaaaattttg taaaaatgga tttctacatt tttgcataaa tttggcttaa
    42061 taccgagaaa ctggcttttg aataccgttt aaggaagagg ttgtaaaatc agcggtttga
    42121 cctgagtatt agagaaacaa acggaaaaat agctgctcat attttgccgt tttttgagct
    42181 aataccgaag gtcagctgat tgaacaccgt ttaccaagag gttgtgagcc taccatttaa
    42241 ctccgaatca caagaaataa tttcaaaaac aacttctcct gtttttggtt cggttggctt
    42301 attatcgaga agtagctact gggagtctat ttatcaaaaa aaggaatgcg acataggtca
    42361 cacccctttt ttattttctt tgaaacatct ttcggtactc gacagcatac aagacaaaag
    42421 attgtaaaag accgtaagtg atgacggcaa aaatgactct cacccacagc gttggtaatt
    42481 ctgggaaaag cagcaaaagg atcatgccaa catagatgac agcagtcgct actttgccat
    42541 accacttcgc tccacttaat ttgatatttt tagatagccc gattaatcca gctacaaaca
    42601 taaagccttc ttttatcaag aatagaccaa acaacagcca aagccacggt cgataccatg
    42661 ccatagacaa aatcagaaaa agttgtgtca atttatctgc aaacggatca agcaccttcc
    42721 ctaagtctgt tacctgatta tatctccgag caatgacccc gtctagataa tcagacaaac
    42781 ctgagacaac aatgaccgct cctgctatat aaaatgactg tctcagcaca aaataaagat
    42841 aaatcggcag caaaaaaata cggaatatcg ataaaagatt cggaatcgtt tgtatttctt
    42901 tacgccagtc tgtgcttaat ttcattttat cgccctacct cctcagcact cttttctttt
    42961 tattttagct tacacgggga ataaaaccaa tgatttcact gtttttattt ttctttacag
    43021 acactgatat tatgagctaa tttgagataa tcacttgctg ttttcatcgt ttactcttct
    43081 ttcttcctgt cgtttgcgca tttcttttac tagtacttta tgagcttttt ttgatcctcg
    43141 ctctaggtca cgctgccgtt tacgtgtttt ataatcgata aacagactgt ctaaatcgta
    43201 acctgtcggg tacagatctt ctgctgaacg caccagacga acacgctgac gaggcacgtg
    43261 gattattttt ttgtctaaat atacatcaat cgtatcttca cctatatctt cataaactaa
    43321 cgcttctttt tggtattcat tgacaaatat ccgatcacct tttgcaaaga gctgacgttc
    43381 ttttttattc tccttcctat ttgtaactgc tgtcttagga agaaattctt ttttctgtgt
    43441 ccgataggct ttttccgcca agtaacgctc tgcttgatgg atcaatttca tcgacatatc
    43501 cattttatga gcaatccaca aagcttggct ttctcctgtt tcttccaacc cgcaactgat
    43561 atttaggacg aagtgcttcc cgatcgaaag ccatagcagc cggcacaaaa tcttcatgat
    43621 ctccggcgaa tttcttgatt tctccataat gggtcgtggc aatgatcaat gccccttgct
    43681 catacataga ttccatgatt gcgatcgcta gtgcagcccc ttcatttggc tctgttccac
    43741 tgccaatctc atctaaaagt accaacgtat tcctttttat ctttttcaaa atagcagcga
    43801 tgttttgcat atgaccagaa aatgtactca acgcattttc taaattttgg tggtcaccga
    43861 catcgacgaa aatctcatca aagacagcaa gctctgtccc ttcttttgcc ggaacttgca
    43921 taccgaacat cgccatcaaa gtcaataaac cgactgtctt caacacgact gttttgcctc
    43981 cggcatttgc tccggtgata atcaagcctc gatagtcttt accgatttca acatccaatg
    44041 gtaccgcatg ctcaggtaaa aatggatgtc tgccttgctt gataatgata tgttccgatt
    44101 tattgattct aggtgtgctt ccattgattt ctcggctaaa ttttgcccga gcaaagatga
    44161 tatctaacac cgtgatcgtt tcgatcaata aatcgatcac ttcttcgttt tctgcgatag
    44221 caccagtcaa tgccgccagg acttgatatt cttcactgat ttcttcagct tttaacaact
    44281 gatagtgttc attcaattta gaaatcacag ttggttcaat aaaaaccgtc gttcttttat
    44341 ttgattcatc gataatcgtt ccatctattt tatttttata gctggctttg ataggaatcg
    44401 tatagtattc gcctttttga acaatcattg cttcttggat catttctttg tttttcggat
    44461 gacgcaagaa tttcagtaat tttgactgga tttctttttc gattagttgt aattgtttgc
    44521 gtactttacg caaattacgt gacgcgtcgt cactgacttt attatttttt attttctggt
    44581 caatcaactc ttctacattt atcaaatcag gaagatgttt gctataagca aacaacaagg
    44641 gtgtctggta ttgattctta tcaaaaaact ttgtgatcat ccgactgctt ctaagaaaat
    44701 ccgcatattc gatcaaatcg gttggctgca gaatcaaccc ttttttgact tgttctgtca
    44761 gtgcatcgat cctcggcaac cccataaatg gaacatgctg actgctttct aagatcaagc
    44821 gtgcttctct tgtttcttct tgacgatcca agacagtttg taggtttgtc gagaccgtca
    44881 tatcgctgat tcgttttttg ctatagtttc caattgctct tgcttggatt tcctttttga
    44941 tccgatcaaa ttgtaattga ctaatcattt cgttgtccat attctctcct tttcttgaag
    45001 agatacctgt cccaaaataa ataagggcct agagataaat aaaaggatag aacacagctt
    45061 aaaaaagtca tgtcctatcc ttattagaat acaaagatat tgttttcctg acaggtaccg
    45121 tttcataagc acaccaaata aactttgttt attggctatt cgacttactg attagatacc
    45181 agctacactc acaaaacata ccccattctt ctaggtctta tttattttcc acttaactat
    45241 agcaagacac attttggttg tcaaacttta gtatgtttta tagatttaca ccattaatga
    45301 atagacacct accgcgataa ttgctccgat cattggacct gcgattggta cccatgaata
    45361 tgcccaatct gaagttcctt tattagctat tggcaatacc gcatgtgcta atcgaggacc
    45421 aaagtcccga gcagggttga ttgcatatcc tgttggccct cctaatgaaa gaccgatggc
    45481 taagatcaat ccacctacag cgaatacgtt cgttccaggt gcaaattcgt tttgtccaaa
    45541 agctagcaac ccaagaacta atacaaatgt accaattaat tctgtgatca catttgctgg
    45601 ataattccgt atagcaggac ctgttgcaaa cgttcctaaa atcgcgcttt catctttcgt
    45661 tgcattccaa tgtggcaaat aagaaagcca aacaagaatg gcccctgcaa aagctcctaa
    45721 aacttgcgca atgatgaaag gaacgacaag attccaacta attgcccctg tcatcgccat
    45781 agctagagag actgcaggat tcaaatgagc tgggcccatg aatccagaaa tatagacagc
    45841 cattgttaca gcgagtcccc aaccaaatgc gatgacaatc cagccagaag cctgtgcttt
    45901 acttttgttt aaattcacag ccgcacacac accatctcca agcaaaacta ggatcagtgt
    45961 cccaataaac tcccccatga tttgagtcat atcactgttc atttatttct ccccctttag
    46021 ttcccgcaga tctgattctg caatcaatgc ttctaaacgt ttagtctgtt gcgccttctc
    46081 ctcttctgtc cattcaaaat atccagcaat tgcatcgatc acaggttgct tgatctcatc
    46141 aagctgatct cgttcaaaca acagatgatt tgtacgacga atgagataat cgcctggggc
    46201 caaaaccatt tcttcttcta aaccataacg taaccgagct gactcagcga gactcaaacc
    46261 aggataaggt gccatttctt ttgctaattc aaagatacgt cgggcattag tcccgtaaaa
    46321 atcagcgata taagtagcat cttcttcctc taaaccggct gctactcctt cttttgctaa
    46381 ttctgtcact gtttcttcta atttagttgg atcgaagttt ccacctgaaa tctgatattt
    46441 tttagagtcg atttctttcg tctctattcc gtattcttct tttaataatt ggcgtatcaa
    46501 tcgcaaagct ccttctgcca tcttacggta atcggtgatt ttcccaccag ataaagtcag
    46561 caaaccatct ggttctcttt ctaaagaact acctctggaa atcgtagaag gtgctttttc
    46621 atcacggctg ttttccaaat ggttcaacac gtcttctact tcagcacgag aaactttgtt
    46681 ttccttatat tcacttactg tatcgacaac tttattgaag ctcttgtctg aaatcgatcc
    46741 attatctcca ccattgtaat cagaaccaga attaccaatc aacagtggac gaagtcctgc
    46801 ccagcttgct tcgatatcat tcaatgtgat atttgcttct ggatagcgat gattgatcac
    46861 atccaataga taatctacat cttcttgtgt gactttcgga tccgtaaaat ctccttggta
    46921 atccgtatct gtcgtaccaa agtaagtctt gttttctctt ggaatagcaa aaaccatccg
    46981 cccatcttgc tttcctgtat cgaagtatgt gggttgcggt acaggcagtt ttttcgcatc
    47041 tacaactaaa tggatccctt tagttggacg cattttagga gagactggac gcgtaaaatt
    47101 caagtttcgt actttatcta cccaaggacc actcgtattg atcactaatt ttgctttgat
    47161 ttcgatcact tcatctgtca gcaggtcacg agctttgacg ccaacgattt gatcgccatc
    47221 atataaaaag ccaaccgctt tcattttact tactagataa gccccatctt cggcagcctt
    47281 tttgatatta tcaatcacta aacgggcatc attgttgcgg aaatctagat agacacccgc
    47341 gccttttagc ccttcttttt tcaaaaacgg ttctctttcc aatacttctt ctggtgtcag
    47401 cgtataattc tcatatttgg ttcctgtcac atttgccagt ttgtcataaa ggtccattgc
    47461 tactttgacc gagaacatat tgaaggttgt tgctccttcg ccctcataga ttggcaaaag
    47521 cattggatct ggttttggga tgtgtggggc aatactttgt acgactgcac gttcaccaac
    47581 tgtgtcagct actacttcca catcaaatgt cttcagataa cgaataccgc catgaacaag
    47641 tttggtcgag cgagaggaag tcccttctgc aaaatcctgc atttcgatca atcctgtttt
    47701 gattcctgat gctgctgact ggactgccac accggcacca gtgattccgc caccgatgat
    47761 caacagatcc aattcttctt gctgcatttt ttgaatgcta tcttgtcttg tcttgtttga
    47821 aaacatatgg atttcctcct tattcaaatg cttgggtagc ttttactgct tttttccaac
    47881 cgttgtaaag tttttctcgg cgctcttcct ccatttgaac ttcaaatagc ttgccagctt
    47941 cataaaattc gcggatctcg tctgtatctt tccaataacc gacagctaat cctgctaaaa
    48001 atgctgcccc tagcgccgtt gtttccaagt tttctgctcg ttggatcggt acattcaaga
    48061 tatctgtttg gaattgcatc agatattcat tgtttgctgc accgccatct actttcaata
    48121 caggaatctt gattccagtg tcatcttgca tcgtatcgat gatatctctc acttgataag
    48181 caatcgactg taaagtagct ttaatgatat cttcttttgt cgtaccacgt gtcaagccaa
    48241 acatagcccc tcgtgctgcc tgatcccaat aaggagcacc tagcccaacg aatgcaggaa
    48301 caacataaac ctcatcttcg cttgtagagc gtttcgctgc gtcttctgaa tcgctagctt
    48361 tttgcaacat ttgaagtcca tctctcagcc attgaatcga agaaccagcc acaaaaatac
    48421 ttccttctaa agcatagtaa acctttccat ttatgccgta accaattgtc gtcaataaat
    48481 tatttttgga taattgcggt ttttcaccgg tattcatgac aataaatgaa cctgtaccat
    48541 aagtattctt gaccatgcct ggttcgaatg ccatttgtcc gaataatgcg gcttgctggt
    48601 ctcctgccat ccctgaaatt ggtgcttcgc ttccataaaa atgataacct tgcgtataac
    48661 cataaacttc tgagttagaa gtgacttttg gtaacatcac tcgaggaata tttagtaaat
    48721 ctaaaatctc ctgatcccaa tctagctgat ggatattgaa caacatcgtc cgactggcat
    48781 tcgagtaatc agtaacatgt gcttgtccat cggtcagttt ccatactaac caagaatcga
    48841 tcgttccaaa catcaattcg cctttttcag ctcgttcttg cgcgccttct acatgatcaa
    48901 gaatccagcg tatcttcgtt gctgagaaat aggcatcgat cactaaccct gttttttcgt
    48961 ggaaaaattc ttgatagcct tcatttttta agttatctgc aattccagct gactgtctgg
    49021 attgccaaac aactgcatga tagattggac gtcctgtatc tttttcccat attactgttg
    49081 tttcacgctg gttcgttatg ccaatcccag caatctgatt tggtttgatc cctgattcga
    49141 tgaacgctcc ggcaataact gactgtacag aattccagat ttcatttgca tcatgttcca
    49201 cccaactttc ttgcgggaaa tattgcgtga actctttttg agaactgccg atgtgtcttc
    49261 ctttcttgtc aaagataatt gctcgtgagc tagttgtacc ttggtcaatc gccatgatat
    49321 acgttgattc tgtcattgtg attccctcca ttatatgcaa acgctttctt taactactct
    49381 tatcatacta ctgttttaaa aaaagaaact gtcaaaattt tattcttttt ttcttttttt
    49441 gacagttttt tattatgaag ctatttttta atcaattctt ttcttttttc agcgtctcta
    49501 ttcttctttt caatcgaata atgtcgtaag gtgacgcaaa ttctgacaga taatattcac
    49561 actgtttttt gtttctctgg caaaatgtga tcaccaaatc atattcttta ccttcttcat
    49621 aacttccaat ctcaataccg ccgattcccc tcagttctct gatcagatat tgttgaaaag
    49681 caatacgata aatcggcaga ctttctaaat cgattccaat cactactggt tttgcaatat
    49741 aaaaatcgat catcaaaaga acattggcat actggatcat caggtaacta agctcactat
    49801 cttcagctct tttcctcggc aattgctgca ccgctgtttc ctgtaatttc gccagtaact
    49861 gtgttaagga atgcccgagc attttctgct gctgttccaa aagccgatca cgctcataga
    49921 cttcgatctt tccaacaaag aaaaaccatt cattgttgat ctgggaaatt tggtagccta
    49981 ctgccttttc ttctttgatt ttcaaacgac gaggacgata atgccataac atcgtttccc
    50041 gaatgtaagt atctaaaaca gcggttggta actttttgct ccgtgtcaaa tcatactgat
    50101 aaaatatttc ttcgtctact atcgaaaaag aaacaaaaaa gctgtaaaac atcattgttt
    50161 cgtatacatt cagttctgct gctgttcgac taagatgcat cacgatgatt ggatcgattg
    50221 cttgataaag ccggtccgac tgatagagta atttcttttc tttcaaagtt gcgtacgtac
    50281 ttttttcatt caatagtctt tttttcgtaa tcccgagcca gcaagcaatc tgtgcttctg
    50341 ctgatgccgt aaacgttgtt tctaatacac ggtccaatcc tagaatgaac ggtctttttt
    50401 ctggtgtatt ttgcaaaaaa agcggtcgct gatcttccgg gatatattgg aatagttcat
    50461 aataaaaata tcgtatctgt agctcttctc cgtataattg accgtttttg atttgtaatc
    50521 caaattcctc taagagctgg ttcaattctt tgatctttcg aaaaacagaa gactcactga
    50581 tattaaacgc tgtcgctaat cgaacgatgg atacttgctg atgttctaat aaatagacga
    50641 gcatcttaaa cttcaagctt tcctgcaata aaaacaatag aattttttcc aaacttacat
    50701 cttcgtccaa cacaagcagc gcctgatact catttcgctg tacttctact tttttttcca
    50761 tcattcggcc aatcaattca atatcttcca aatattgatc aaacgaggct tttgacagtt
    50821 ggacatgctc tctcaaacca ttgatagagg ctgttccccc ttgcagaaca aggtttcgca
    50881 aaatcaggat ctgtaactgc tcttttcttt ctaataatgc ttcaatcttc ataggcagta
    50941 ttcgccacct ttgaccacac catatctggc acacggtatt ctggctggaa aaatagctca
    51001 tgttcttcta actgttcaaa cgtccatcct tcttctactg caattgtcag ttggtcaaga
    51061 aggtggaaca tctctttttt gctttggagc tgtgctccaa gaatcttttt ttcagaatca
    51121 aatagaaatt tgagtttaac tttgctttga tttattgctg taagcttgta gtctacgtat
    51181 cctgtttcaa ttttttgggg ataaaaaatg ctctcttctt ctgtcaaacc tacgcttcct
    51241 aagaaatagc caaaccactg attgccgact ggacgatagg ttccctgatc ctttgtctgt
    51301 gagccactaa ttgtcttgct gacatttcgt gctgtacgga cagcgttatt tactagagaa
    51361 acatacattt tttcatttgt tggacgaaaa gttacttgga tcgcatcgcc aatcgcataa
    51421 acgtcttttt gagacgtttg caagtagtca tctacccaga tcgttccatc atcattcaat
    51481 gttaattgtt cttcccacat ctgattgtcg ggacgagaat gattagccag caaaacaaga
    51541 tcatagttct cctttctttt ttccgtctct acaatagcgg tttcattttc ggtcaaagac
    51601 acaacttgtt cttctaaaaa aagtgaaagc ccttgatttt tcatttcctt tactaacggt
    51661 tctgtcattt caggatctag ataacgaaaa agaattgact ttcggctttc gtaaagatgg
    51721 atttgttttt ttcttgatgc caacccttca gctatttcga ttcctgcttg tcctgcacca
    51781 ataattgcaa ttttctttgc attcatgatc ttttgctgaa gagccgttac gttttgtatc
    51841 tttttaactg ggtgaataag cgttgattct gcagaaatat ttcgaaactt ttgatttgaa
    51901 ccagtcgcta gaatcaaacg atcaaaatcc aatttttttc ctcctgctaa aacgactttt
    51961 ttttctttca atcctattac tgtcttttct gtgtaaacat ggattcgata gcggtcagtc
    52021 agttcttctt tcgtgatcca agaaagttgt ttttcactgg taacctttcc ttttaatatc
    52081 aaattgaacc cgctgggaat ccatcctaac ttcgattgct tttcaatcaa atatatttca
    52141 cttgttggca tcaattggcg aagataaata gccgcatgaa tccctgcaaa cgacccgccg
    52201 acaattacaa tgttcatctg cattcctctt tcttataagg tgttctactg ctaacttctc
    52261 aatcagaaaa cacagtattc ataactcgct ttcaacctta tgatacatag cgatgcagca
    52321 gaatcaaccc tctccatcat accgtgttct aggtctgctt ctcaataatg tactagtgga
    52381 cctaattgaa gagtatcatt tctgaagaga tgtttcaaaa aatgtactct tataaaagaa
    52441 aaaaactcgc cttttacgta cctctgtcca aataggaaac agaaaagaac gaaaagacga
    52501 gttttagcgt gtgtaatgta atagttagaa tagacacatc atttattttt aattctttaa
    52561 tgcttcaaca actgcttcgc ctaattgatg agcagattga ggattttgcc ctgtaagaag
    52621 ccgcccatca atttgaacca cttctgaaaa agctgctcca gactgatagt ttgctccttt
    52681 ttctttcaaa gcatcttctg ctaagaatgg tacttcattc gttgtaccat ttgcttcctc
    52741 ttcttcatta ctgaacccag tcactgtacg tccagctaaa atagattttc cagactcatc
    52801 tgtaactggc aacaatccta caacaccgtg acaaacagct gagaccagcc cttcatttcg
    52861 atagatttgt gaagcgactt tcccaagatc tattgcttct ggaaaatccc acattgttcc
    52921 gtggccacca gcataataaa ccgctgcata atcatttgca tccacatcat caggacgtaa
    52981 ggtatttccc aaggcccgat ttctgaaatc ttcatcttca taataggccc aatcgatttc
    53041 atccatattc aaaacacttt gcggttctaa aggtacatat ccgcctttag gactgacaaa
    53101 atctgcatcg atcccatttt ttactaatac atcataaaaa tgagtcagtt cacttaacca
    53161 caaacctgta ggacgctcga tcttttcgta cttttctaca tttgttaata cgatcaatac
    53221 tttcattttc ccatcctctt tctataaaaa ttgcgttatt gataacgtgc tttcaagaaa
    53281 ttatataatg ggcttggcaa ataaagctct ttatatgctt cttcaaaagg tttcgttaca
    53341 aattcatcct cacggttttc caataatttc tctgcccagt caggatcgac gatcatttgc
    53401 tgtccaattg ctgcaagttc tgcatatttc aatacctgct ctacatcttc gcgattgcgg
    53461 atcccaccga caccaactaa agggatcttt ccttgaatcg tgttatggac cacttcaaga
    53521 atcgatttat cgttgtattt atcagaacga gctattcgat catacatatt taatgaaaca
    53581 tgcagataat ctagttttga ctcacgtaat cgttcaagaa gccagatcgt gtcttcaaaa
    53641 cggatacctg gtgtttcata ttcttctggt gagaaacgat accctacgat gaatggtttt
    53701 ttagcatatt gttcaaccgc tgagaaaacc gttgttaata attcttcaat aaaacgataa
    53761 cgattttcca agctaccgcc ccattgatcc tctcgtctat tcgaatgcgg agagaaaaat
    53821 tgttggatca aatacgtatt tgctccatgc aactcgattc catcaaatcc tgattcaatc
    53881 gctctacgtg tggctttgcc aaatgcttgg attgtttcaa cgatttcttc ttcactcatt
    53941 gctcgtggtg tttccgcatc aggacgtttt gctgcaactg cacttgcaga tacaggctgt
    54001 tctccagata aagttgctcg acttgtcatt cgtccaccgt ggaaaatctg aataatcgct
    54061 tttgcgcctt ctccttggat tgctttcgct agctcactta atcgaggaat catttcatca
    54121 tgagcgatgc tgagttcacc tggccagcct tttccgatat cttggacatt tgcagcacca
    54181 gtaataacag caccaagacc tttagaacgt tgtttataat aagtgatctc atcttcggtg
    54241 atcactccat tgaaaaagct gagttgtgtc gtcataggcg acagcatcaa tcggttcttc
    54301 aattgaactc ctgaaggtaa agtaattgat tgatcaaatt gtgacattgt cattcctccc
    54361 ataattcgct ctgattaacc tatcgagaat catctatcat taaaccactc gattagttgt
    54421 acatgcaact aatttgctta aaaaatttta ttttttttgt aacttatctg ttgcatcatt
    54481 cattgtctgg gctaattctt tacttgcttc atttcccaaa actgctttgt atgactgatg
    54541 aagttcttcc cagctaccat cgacttcctt agcaaattgg cgtccttgtt ctgtaagata
    54601 aactaatgac aagcgacctt cgctaactgt tttgacgtat cctttatatt ctaatttttc
    54661 aacaaaacga gcaatcgttg atggggcgga atagagcatt tgtgcaatat ctttttgtgg
    54721 tattccatct ttttctttta atatcaataa aataaaacca taagttggtg ccagacctgt
    54781 ccttctaaac gcttcatcag ctaatttatt caacatgcga tctagctttt tcacgctaaa
    54841 gaaaagacat tcatctaacg ctttttcaat tggcttttcc atttcatccc ctccattggc
    54901 gtacatatca tagtatgatt tttatttttt gtcaaaaaac acggaataat gtccgcctcc
    54961 tctgtctaga tttgaaaaaa agaagtagcc atcttcaaag aatttcggct tgttttcgac
    55021 aaaactcctt ctggaacacc gtgaatcaga ggttgtgaaa aagtcgttta gttttgagta
    55081 tgaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaatttgg cttaataccg
    55141 aaaaactggc ttttgaacac cgtttatatc agaggttgtg aaaaagccgt cctgcatcaa
    55201 gtaataagaa cagccaaaca aaaatagttc ctcatatttt gtcgtttttt gagctaatgc
    55261 cgatggtcag ctgattgtac accgtttatt gagaggtcgt gagcctgccg ttgaactccg
    55321 agtcacaagg agcaatttca aaaaacagct tctcatgttt tatgactatg gtgacttgtg
    55381 atcgaggagt tggcaggaga atactgttta ttcgtaaaga aggggctgtg acaagacttt
    55441 tgtcacagtc ccttatttcc ttttattata ggatttcgcc atttgattgg atgacttgtc
    55501 tgtaccaatc aaatgatttt ttctttaacc gtttctttgt gccattccct tcgtcatctt
    55561 gatcaacata gatatatcca taacgttttg acatttgtga agtagaagcg ctgacgatat
    55621 caatgcagcc ccacgacgta taaccaaata aatctactcc atctttgata gcttccttca
    55681 tctgctcaat atgctgcttc aaatagtcaa tgcggtaatc gtcttgaacg gttccatctt
    55741 cttctacttt gtctatagct ccgagtccgt tttcagctat gaacagtggt ttttgatacc
    55801 tatcatataa ttgattcaaa gcaattctca aacccttagg atcaatctgc cagccccatt
    55861 cacttgatgg aagatacgga tttttaattc ctccagttat taagtttcct tggacccctt
    55921 cccaattttc tggatgagca ctgactgcac tagacatgta gtagctaaaa gaaacaaaat
    55981 caacagggta ggctgctaag atttcttcat cacctacttc tatttttaag ttgacacctt
    56041 tttcttcaaa cattcgttgc gtatacgctg gataatatcc tcttgcttga acatcactaa
    56101 agaacaaaga agttctttgc tgctcaattg ttttttgaat atcatccgga tcacaagaat
    56161 acggaacaag aagctgatag ctgatcatac aaccgacttg tgaacctttg ataatttcat
    56221 gacaagcttt tgtagctaaa gcacttgcta taaactgatg gtgtgcacct tggtatttcg
    56281 cttgttccaa atggggatgg ttttcttcaa ttactcctac gctaacaaaa gggtgatgtt
    56341 ttacacagtt gatctcattg aaagtcatcc aatattttac tttattttta tagcgtttga
    56401 atactgtttc agcgaaacga acgtaaaact gaataagctt tcgattaggc catccaccat
    56461 attctgtaac caaatataac ggcatttcat aatgggaaag agaaacgact ggctcaatcc
    56521 catattttag acattcgtca aacacttgat cataaaaaaa taatcccgct tcattgggtg
    56581 tttcttcatc accatgagga aaaattcttg tccatgcgat agacatccga tagcatttaa
    56641 aacccatttc agcaaataaa cggatgtctt ctttaaagtg atgatagaaa tcatttccgt
    56701 gtcttttagg ataataatac ttgctgttgg gatcctgcgc cgcttttact tgctgatgcg
    56761 tcatcatatg ccattgatct gtccattttt ctttaggtaa atcgctgtcg aatgtataca
    56821 catcagacac agataatcct ttttcatcta catcccatgc accttcacat tgattggccg
    56881 ctgtcgctcc gccccataaa aatccttctg gaaatttttt catatcgatc cccctaaact
    56941 tgctagtttt cttgtttgat tatttttctt atgactagta atgatatttt tgaatctact
    57001 actatttgta acaataacca ttgtggtcgg atcatatccc tctttgatta attcttgcaa
    57061 atctacttcc atcaataaat cgcctttacg tacttttttt ccatcagata ctgcactttg
    57121 aaagtatttg ccatttaaat tcactgtatc aataccgata tggatcagta tctctgctcc
    57181 ttcttcactg actaccccaa ttgcatgttt cgttggaaaa actgttgaaa cttccccgtt
    57241 tacagaactg tagatttttc cgtccgttgg aatgatcgca aacccttctc ctaaagatcc
    57301 actagcaaat gttggatcgt ttacttcaga taaatcaatt atttcacctg ctgcaggagt
    57361 acttaacact atatcctttg aggattcttc ttcgatttca tccttgtagc cgaaaagaaa
    57421 agttagcata caggaactga tcatcgctaa gaaataacta gcaactagcc aaacaaatcc
    57481 ttcaccaata taaatcggga aagacaaaat acttcttgta gcaactgcca aagcaccggc
    57541 tcctgcggca ccagctactc caccaaatat agctccacat atacaagcaa tgtaaaatgg
    57601 tcgtttatat ttaagagtca caccataaac agcaggttca gtgattccaa ataatgctgt
    57661 gataaatgca gataaagata aactttttac tttcttattt ttcgttttca aaaatacccc
    57721 aagtactgct cctgcttgcg cagctacggc agtcatacat gctggaccta gcgtatcacg
    57781 accgtagaaa gaaagattgt tggtcattac tggtacaagt ccccaatgta acccaaaaat
    57841 cactaacact tgccaaaatc cgccgataac agcaccagat aaaacagggc taaagttata
    57901 aacagctaaa tatccagctg caatcagctg acttgcataa tctgcaattg gacctacaac
    57961 taataaaata attgggacca tcacgatgat acaaatgaat ggtgtcaata aatttcgaat
    58021 actttcgtgc aagcttttgc gaatcttagg ctcaatataa gaaagaaccc atacagctaa
    58081 aattgctgga ataaccgtac ttgtatacct tgctagaatc acgggcatcc ctagaaatcg
    58141 caaggttatg ctatcactat atgcactgac aatcgtagga taaacgagag ctgctgcaat
    58201 aaccatagcg atgaaacgat ctgtattgaa cttttttgca gcagtatatg ccaaaaatat
    58261 aggcaagaaa tagaaaaatg catcagctgc tgcattcaaa atgatataag ttcctgattc
    58321 agtagttaat aaattcaaac tcgtacataa ggcgagtact cctttgatca aaccacttcc
    58381 tgccaagacg cctaaagtcg gggcaaaaac agcggcgatc atgtctataa aagagtttaa
    58441 aatgcctttt ttctcagttc ctctctttcc actagatgca tcatcttcta gtttcatttg
    58501 tttggataat acgtcataca cttcatttac tgcatttcct actacgactt gaaacattcc
    58561 tccagcttcg actacagtga tcacaccttc tagttgctct accttctcct tatctgcctt
    58621 cttattatct tttagggtaa agcgtaacct tgttgcacaa tggtaaacat tgctgacatt
    58681 ttcttcgcct ccgacaaatc ggacaatatc tttcgctaat tgtgaatagt ccatttttac
    58741 ttcccccttg ttaattaatg ccgtagcaat ccttcttaca agataagtat agttcttaga
    58801 aaacggtttc tccattcaat tagtacccct tctaatagcg aacgaaactg tacaatagtg
    58861 tgtatggttt acttagccga gcaaatcact cactgcttga aagaaaacaa gtatatcgtt
    58921 tctcttatag tcaaaggaga caaaaaaaac acgcttgatc ttcctttgct gatgtataag
    58981 tggtatctca agctgatact ctgtgatttt tgtttctact tggcttttga taagacagca
    59041 caacaaagtc tcggcattta acggaagcat aactaggtca gaactaggag agattagttg
    59101 ctgaatggaa tctatctttt tatggatttt tatttgattt tcttctttgt agtaatgatc
    59161 aagcagtttt gttttctttt tttcttcaac tatggtcagc ttttcacgca aaatctgata
    59221 aatctcccaa gattcaaact catctagaac actaggtaga taaatcaaat catcccactc
    59281 aaaaataagc tcttgctcag tagtcaaaat tgcatcatat tccgttattt tgtttgattg
    59341 atttttgaaa agatacactg gttctatccg gcattctttg atccacatgt tcatactttg
    59401 ttgaatctgt ttttttagtg tattcaaaat actaattggc tggttactaa caatcagtat
    59461 ctccccttgt tttcgaaaag gattcaacgc ttcggcaaga tacaaaacaa aataagcgct
    59521 ttcagctaaa ttgggacgta attgaaaatg tattgggaag aaacgccgaa ttaaataaat
    59581 ttctaaagga taacgggaga tgatcttttg tacaaaatga ttcatggtat tatgtccggc
    59641 atccatcctc gttttcattt gtttgatatg ggttagcaaa aattctgttt catggaacaa
    59701 taacgaaaca ggaagatcta aaaaacgaat aatcgtttgc tcaaatgctc ttagattctt
    59761 ctctatttct ttgttttttt catcattaat catcaaataa tttaactcta atagtcgatt
    59821 ttcaattgct tgtttttctt gattggaaaa agaatagggt agttctgatg ataacttttt
    59881 tgtgagggtc tctaccatcg gaataaataa tgtatacctg tttgtttttt ctaaaagttc
    59941 ttctcttcga agaatggtca aaacgagata ccgagaaaat cgtatgaagt cctctcctga
    60001 aacaatataa ttatatgata taagaacctc ttttaatgtc ttttggacag tcggcatgat
    60061 attggaaaaa attgtactga tttgttgagg caaccttgcg tgttggatca ccgtttctgt
    60121 tccaacaaga gataagaaaa ttctttttga attttcttca gcaatgattt ttagtccttt
    60181 tgtcgacgaa acttccagtt ctaatttagg attgcgctcg atccaacgca aaatcgtttt
    60241 tatttgtaat gatacggctg tttttgaaag aaagaaaatg tctgctatct tttgcatact
    60301 gacataccct ttttgaaaca ataagtacgt cacaatagtt gaagctcgga aatccatgct
    60361 ggaatagatt ttatgtttcg tcacctcttg tatgatttgc tgtttagttt cagattcaat
    60421 tgattctaaa aaataacctg aacgtttatt tgactggata atgattttct tctcgtagat
    60481 agcaagcata tttttgattt cagtttggat cgttttcttg ttggtaccta aagcttcagc
    60541 taattcttcg cttgttatcc aatgattctt atttcttaat actacaagaa tttggagttg
    60601 tcgtttattt aagtccattt gcttacacct cctctttgtt ataaacatac aggaaaaacc
    60661 tctcaaatga caatagtcac ttgcagaggt ttaaaaatca tttgaagaaa gatctatatt
    60721 caaggttttt accattagac tggataattt gtttataata ttcaaaacta tcttttttca
    60781 atctcctaaa gcttccgctc ccatcatttt gtctatccac ataaataaag ccatatcgct
    60841 tcctcatttc ccctgttcct gccgatacga tatcgattgg tccccaccat aagtaaccaa
    60901 tgatttggca accatctttt attgcctcag caatttcctg aagatgtatt tttagatact
    60961 cacaacgttt tggatcgtgg attcgattca attcatctgg tgcttcatct agtccaatgc
    61021 cattttcaac tatgaataat ggtaattcat aacggtctgt caattcattc atgacataac
    61081 gaaaccctag aggatcaatt ggccaactcc aaggttcggg agatttttcc ttcaaaaagg
    61141 gattatcgaa accatttgct ccgccagtat ctacgcgcat ttctgcttct tttttataga
    61201 cagcgctacg ataataacta aatgcaatat aatctgccgt gttagcttgc aagcactgtt
    61261 cgtctccatc taacattact ggtgcacagt cttcttcctt ccatgtcctg taaatataag
    61321 ctggataatg ccctcggcac atgacatcgg agaaaaacca tgaattgcgt ttaaattgca
    61381 gtgaaccaaa tacattctct ggatcacagt catatgcata agtcgctaat gcagaaaagc
    61441 tacacataat acccatctta gcatttggat cgattttctt taataattga gtagcacggg
    61501 cgttcgctag aaacatatga tgacaggctt gataaatcac tttttttgtt agtgtagcaa
    61561 tgggttcaag tttatctcta gcagggaatg taaccaatcc agcagctgca taaggcatac
    61621 gaaaagcatt attgatctca ttaaaggtca tccaatattt cactttccct tggtagcgtt
    61681 caaagacggt ctgcgcataa aattcaaaaa agtcgattaa ttttcgattt tgccaccccc
    61741 catactctcc taccaatgct aagggggttt cataatgact aagagtaatt actggctcga
    61801 tatcatatcg atttaatgta ttgattagtc tgtcataaaa tacgagacca gcctcatttg
    61861 gcttctgttc gtctcctctt ggaaatattc gtgcccatga gatgcttgtt cgaaaagctt
    61921 ttaaacctaa ctccttcagt aaacgaatat cctcttcaaa tcgatgataa aaatcaatcg
    61981 cttcatgaga aagataatgg agtgattcgt ctaaagcaat ctcccaaatc tttttttctt
    62041 cattccactg aatactagga tgctggttca tgatcccgat aagcgtatct gtcacattag
    62101 gtaatttgcc gtcttccaac catgcacctt cacattgatt agcagctact gctccgcccc
    62161 ataaaaaatt ttctggaaat atttcctgat ccataactat ctctcctcgc tatcaacaat
    62221 ttcttacttg aagtatagct cgattcatca aaacaagata ttctaactat ttcggcagaa
    62281 gagaagaaaa aaattgaatg ctactcttca tttttaatat ttatatataa caaatacata
    62341 agacgatccc cgaaagaaaa cactcgttaa ccatctaagc aaacgattta ttttacttcc
    62401 aacacaaatg cttcataagg tcgtaaatca actttgccgt ctggtaactg tttcagagga
    62461 taatttgaca caataaccct taaagcttca cgtggaatag tgaacgtctg cttttcactg
    62521 gacatattac aaataacgag ccattgctgg tcttcatata tgcgagaata ggcaaagaca
    62581 tgctcaggcg tcttttccac taattcatag tctccccaga cgatgatgtc atgttttcta
    62641 cgcaaatcga tcaatttctt gtacatataa aaaacagaat cttgatctgc taacgcttct
    62701 tctgcattga tttctttata atttggattg actcgaagcc atggaattcc agtagtaaac
    62761 ccagcatgtt cacgggcgtt ccattgcata ggcgtcctag cattgtctct tcctttcgcc
    62821 ttgattgatt ccaagatttc ctcttttgta aatccagaag aaatcctttc gttatacata
    62881 ttgattgtct caatatcttt tgcttccgcg atatcatcga ttgggcaatt cgtcatgcct
    62941 aactcttctc cttgatagat ataaggggtg cctttcatca tatggagcaa gatagcaaac
    63001 atcttggcac tttcttttcg atacacatcg tcatttcccc aacgagaaac aattcgcgga
    63061 agatcgtgat tattccaaaa taaactattc catccttcat cacctaaaga tgtttgccat
    63121 ttggataata cttttttcag ttcagagata ctcaagggtt tcaaatccca tttgtctttg
    63181 ccttcttgct gatctaatcc tatgtgttcg aattggaaga ccatggataa ttcatttctt
    63241 ttaggattgg aataaagttt agcgatttcg gtagttgctc cccatgtttc gccaacagtc
    63301 atcagatttt tgtcaccaaa agttgcttga ttcatttcct gaagatactc atgcagttta
    63361 ggtccattac ttgtgatttc tttatccggt tctttgccaa tcatatcgat cacgtccata
    63421 cgaaagcccc caataccttt atcgatccag aaattcatca tttgataaat ctcttggcga
    63481 acctgtttat ttttccaatt caaatcaggt tgacgcttgc tatacaagtg cagaaaatat
    63541 tgccccgttt tttcatccaa ctgccaagca gaaccactga atattgagcg caatctatta
    63601 ggctcttttc cattgaccgg atcacgccaa atataatagt cccgataagg attttcttta
    63661 tttttacggg cctcaatgaa ccacggatgt tcatcagaag tatgattcac caccagatcc
    63721 atgatgatac ggatatttct tttctttgct tcttcaatca gttcttccat ttcttccatc
    63781 gtaccgaatt ctgccatgat tgcttgataa ttactgatat catacccatt gtcatcattc
    63841 ggagactcgt atactggaga aagccaaatt gcatcaatac ctaaatacgc cagataatcc
    63901 aacctggaaa taatcccccg caaatctcca ataccatctt gatttgaatc ttgaaaactt
    63961 cttggataga tttgataaac aactgaatgg tgccaccatt tttgttccat gattttcctc
    64021 ctataaatgg atgtttctat tttttgctta atcatttatt ttagtctctc tcactaaaaa
    64081 ttgtaacgct ttcaaatagc tattgcacct tgttatcggt aacagaataa aacaaaaaaa
    64141 cagctgagga aattccctca actgtttttt gaaaggattg atttatgcag ggaagaatgc
    64201 tgatttcaca acttctttat aaacggtgtt cactcagctg tcccttggta ttagctcaaa
    64261 aaacggcaaa atataagaaa ctattttttc gtttttttct ctaatactca ggccagaacg
    64321 ctgatttcac aacctcttta tttcacattc aatatccaca tgaatcccac aaatacaaag
    64381 aacagaccat acatgattgg agatacttct tttcctcttt tagctgctaa catggtaatt
    64441 ggatagaaga taaaacctaa tgcaatccca tctgaaatac tataagtcaa aggcatgccg
    64501 atcaagatca agaatgaagg gatcgcaatc tctaagtttc cccattcgat ttgtcgcaaa
    64561 gattgtgcca tcaatactcc taccataatc aatgctggtg cagttacttg tgaagtaaca
    64621 actgatagta atggagaaaa gaacaagcct aacaagaaga aaatccctgt agtaatagct
    64681 gtgatacctg aacgtcctcc aacggcgata ccagctgatg attctacaaa tgcaccaaca
    64741 ggagaagtac caaataagga acccgcaagc atagctgttg aatcagaagc taaagctttc
    64801 cctacacgag gcatcttgtt gtctttcatg aaacctgctt ggtttgctaa tccaactaaa
    64861 gttcctgctg tatcaaagaa agtcacaagc aagaatgtca gcactacgac ccacatttga
    64921 agagaattga tgtcccctac atgttttaat gcaactaaaa atgttggttt caaacttggt
    64981 gctgcggcaa caatttcaga aggcattgga atcaaacctg tgaccagacc taaaatcgtt
    65041 gtagctgcca tcccgatgaa gatgccacct ggtacacgcc gaacaagcaa gatagcagtt
    65101 acgactaatc caaaaatcgt caaccaagta gaaccaacgt tgagtgatcc taatcctact
    65161 aatgtagatt cattcgaaac gatgatccca ccttcgctca gaccaaggaa agcgataaat
    65221 agaccgatac cgccagaaat cgcatatttc aaatctgctg ggatcgcatc aatgatcatt
    65281 tcccgcagct tgaagattgt gatcaaaata aaaatcaaag aagcaacgaa tacgccggct
    65341 aatgctgttt cccatggaat ccccattcct aaacaaacag aataagcaaa aaaagcattg
    65401 atcccaagag caggtgccgt tccaattgga taacgggcta atacccccat taaaacacag
    65461 ccgatcgcac tggctaatgc agtcgcagta aacactgctc cttcatccat tccagaagca
    65521 cctaatacag ttggatttac aaataagata taagccattg agataaatgt tgtaaatcct
    65581 gccagtacct cacgttttac cgtggtgttc aatttttcta tttcgaagta ggaaagtatt
    65641 ttttccttca tcttccatcc ccctaaatcc gaacattata aataaagtcc gttaaaattt
    65701 cgatttatta ataaaacaac gtgttttatc atatactaaa acaaaacata agtaaacaaa
    65761 aaattcggat tttaagcaaa atccgaacgt tccatctgat taaatccctt tatctcaaag
    65821 ttcatgaaca ttcactttgg attacaagaa tttctttatt gtgttaataa agtttcttca
    65881 ttctagtctc aaaaatcaat agtgtgattt gattttacca tttcgtttct gttcttcttt
    65941 gatgaattct tttgtatcga caggattcag attatttagt ccgccaaaac ctgtatcgac
    66001 catttttccc cagccttttg tcttcttttt tcctgatttg agattctttg ctcgaagctt
    66061 ctcactatgt tttgctcgcc gatacttcaa ataaccttct aatcttccca ttagatccac
    66121 tgtttttcct cctttttatt caatcagacg actcagcttt tcctataaca aaataattgc
    66181 cttcagggtc agaaaaattg aaagtcaaga tgccattgtt atctgtgatt tctcctggtt
    66241 cttccatgtt ctcgtagata gtttcaaatt catccgtaaa aaatactaaa gaaggcggtg
    66301 gtcctaatac ctctggtgaa aacttctgca caaatacttt tgggaatacg cctaattcta
    66361 attcttttga gatgctgagt acaatactct taaattcttc tggcaattcg attgtttcag
    66421 aaatttctgc accaattttt tctacaaaga atcggcaaat catctctacg tcattgacat
    66481 aaagcatcac acgtgttttt tgtatcatca tttgaccctt ctttctattt gattttcttt
    66541 cctgtctata aactgattcc attatatcac gaaagcgcaa acaccttgtg tttgatacta
    66601 ttcaagaaaa ataaagcaat agtttctagg attgctttcc tccacaaact ttttttacaa
    66661 aaaaaaaaac ggcaatgaat gccgctcttc ttatttcgat tcttttataa ttctgcttct
    66721 gattcatctt tggttagtgt gtgtctaaca ctagttacgc caaatgtttg atcgattttt
    66781 tccttcaacg cttcgattgc ctgtgcgcca cgtgccgctt gttcagcatt gatggattca
    66841 atgaccagca ccgctgcttg tgtttcttcc gtcagcatcg ttctttgggc ttcacgccag
    66901 ttgagacagc gacagatcgc tccttgttca tcatagtaga tgatttcttc cggaagtgcc
    66961 ggagcatcgg aatctgcacc taaaggcagg aatgattcgc caccttgggc ttttcctaaa
    67021 tgcagatttc ctgcgatctt ttcgatatct tctccgccgc aaggtacgcc aaaagacaag
    67081 gaaatactat tatagatatc tacaagcgga ttgattggga aaaattcccg ttcttgattt
    67141 gcacgtttca gtaaagcttc aatagaagaa cgtgcacctt ttttagtctt gaatgtggta
    67201 aatgcctgac gccactgagc aatgacttcg ttttggctga atgtttcttc tgtaaggtaa
    67261 tttcttgctt cttttgctgc ctcattcaat aattgatgat acgtttcttc actttctttt
    67321 gttacgtggt tatcgatacc atttacggat aagatttcga tggttgcttc aggaaatact
    67381 tcccagaaat tggtatccac tattactttt ttcatctgtt cacacctctt gattagaata
    67441 attgcttgat gaatagatag tctgtttggg gatcatctcc catataaaaa acatgttcgc
    67501 tgtatttgat aaatcctttt ttacgataaa aagcgagcgc aacatcgttg tgttcccaaa
    67561 caccgagcca aatggctgtt ttttgctttt cttttgcttt ttcaatagct gtatctagta
    67621 aaaatgtacc gataccttga tgtttaaatt tttttcgtac atagattcgc tcgatttcta
    67681 aaaaatgatc agccatttcc tcagattgac tcgttcctgt atttagtttc aggtaacccg
    67741 caggctcttg atcgatactc actaggaaaa aatcggtttc tttatctttt agctcttctg
    67801 taagtttttc tagtgcataa gcttgtgtca agtaatcagc taaatgctcc ggtgaattat
    67861 cttttccaaa tgtatctgta aatgtttcga tactaatttg ttgtagtaag tctacatctg
    67921 aaggcgtcat ttttcgaatt tcgtatttat tcacggcttc ttcctttcaa tagatccttt
    67981 tattgccttt tttgacaaaa ttccaatctt cgctaacatt cttgcatact ttatttaaaa
    68041 gatattccaa ttgttttgct tcttcgtcag aaagaccatt taatgcgact gcattggagt
    68101 agttgttttc tcgaatgatg aaaggataga tttctgctcc ttttttagtc ggatagagtt
    68161 tcttgatttt tttattttct ttatcttcca aacgttcgat catcccattg gattctaatt
    68221 tcttgattgc tcgggcagtc gttgtacgat ctactttgat catttctgct agtttttctt
    68281 ggatgattcc aggattttcg caaatacgta ctaaataaag atactgtcct cttgttaaat
    68341 cgacttcttt gaattcgata ttgctaatag aatctagcgc tcgggcaatc attccgatgt
    68401 ctcgaaggat ttcagccatt tcatcccacc tttattttgt ttgtaagact agttcacctc
    68461 tttttgttgc aaatgcaact attttcaata aaaaaatcaa gttctttttt aataactagt
    68521 aaagtatatt cttttcttct tttacgaaaa agctcaatca caatagatct ggtttttgtt
    68581 ttctaaaata agtaactgtt tcttcgttgt tcgcttttct gatagctttg gcaactcttc
    68641 tatactaaaa aatcccatgt tggaagtttc attattctca ataaacttgg cctcttcacc
    68701 gtggatcgca caagcaaaaa caagtttata atactgaaaa agctgtggaa tatctttttg
    68761 tttattcgta tcaaaaactg ctcgcaactg agtagtttct actgttaatc ctgtttcttc
    68821 atagacttct ttacgcacat tttcttcagg agacaatcct atttcagcaa atccaccagg
    68881 aagtgaccat tctttcgtcc gtttatcttc gattagtagg accttgccat cttttttgat
    68941 ataggcacga acatctactt ttggtgttgg atgtccttct ccaatcgttg tgagcgcttt
    69001 tatttcttcg aaagtttctt ttcctatttc actgattagt tccaatgaaa tcgtccggat
    69061 ctcctcaaaa cgctctttat caaatacatc ttttccataa tatagaccag cttgagaaag
    69121 tgcgagtagt cttttgtatt gagccaactt cttgttcatt ctcttccctt cttttttagc
    69181 aaatgtatga ctatgtaatc aagtatagaa gactggttta attacggcaa ataaatataa
    69241 tgaaatctgc aataaataaa agaatgttaa aaaactcgag aacaaaagac attcgacttc
    69301 tctggggctg tgaataaatg gcgttcaaaa gcttgcatct gcggtaataa gccccaccaa
    69361 acgaaaaata tgacgagcta ttcttgtctg gctgttctta ttaattgagg caggacagct
    69421 ttttcacaac ctccccacta aacggtgttc tcctaccaac tcctcggtca caagtcacaa
    69481 tattcatcaa acatgagaag ctgttttttt gaaattgctc cttgtgactc ggagctgaac
    69541 gacaggctca cgacctctcg gtaaacagcg ttcaaaagcc aatttttcga tattaagcca
    69601 tttctttgtt acttattgtc attgatttct ttttctaatt tatccaatag tggagtgtaa
    69661 tccacttgag tatagtctgt tacatcaaat tcatatagtg ttcctaactg gaatttgtca
    69721 tattttcggt cggcgatgat ctttttgaat gcttctttcg taatatgatt ggtccctttc
    69781 gttcgatctt ccaatacgtc accatagtga taatgatcca tgatatagct caaatgacga
    69841 tctgattcac gatcacgttt gtaccgacgt tgccataaca cttcaaagtc tgctgttagg
    69901 cgaatcgtga tcacctgata tccgtatcta tctgctaatt cttggagtcg tgctttttgc
    69961 ttatcactaa aaggatactc ggacacaatc actttctttc ctgcttccat atacaaatca
    70021 agtgctttgt aatagaaagg ataaactttc gtcctttctt gttccgcccg ttcttccaaa
    70081 ttgttaaacc cgatggattc tgcatagtat tccttgattt catcgagcgt gatggcatac
    70141 atatctggaa atcgttcccg tagtttattc ataagatatg tttttcctgt accaggacta
    70201 ccagccagaa gaatgaaata ctttttcatc tcatttattt cctaagttaa ttggactttc
    70261 caagttcata aacagtccaa tcttatgctt gacaatttct tttactgctt catttgctgg
    70321 aatcacatta tggcgaagag acttgttgat ttctgtttca ctaaaggttt ctttagctac
    70381 tttcgtccag ttgactaaaa gctccgttcc aacattgaat ttgcggatcc cgttattgat
    70441 caattcagga taatcttctt ctttcacacc tgttcctcca tggataacta gggggacatc
    70501 aactactgca tcgatttctt tcagcaaggg aatgtttact tctgtttttg atttgaattg
    70561 tccatgattc gtaccgatag caatagctaa tgcatccacg cctgtagctt ctacaaattt
    70621 cactgcgtct tccggtcttg tatatacctt gtcattttca gcgacatgga ttccttcttc
    70681 tgtaccgccg attgttccaa gttctccttc tacggaaaca ccgtgcgcat gggcatattc
    70741 tactactgct tttgtcttca ggatattttc tttgaaaggc agatgggacc catcatacat
    70801 gacagaagta tatccgctgt cgattgcttc tttgatatca tcaaagtctc gggcgtggtc
    70861 taagtgcaag actgcatcaa ccatttcttc ttctgcaata gctgtcatta cattgactaa
    70921 cacatgcatg ccgatatatt tagcggtatc gacacttgtt tgaataatga tcggcgaacc
    70981 catttctttt gctgcgccta gcatagcagg aagcatttct aggttatgcg tattaaaagc
    71041 tccaactgta aaattcaact cttctgctgt ttttgttact tctttcaatg ttgtatacat
    71101 cttttattgc caccttatct ttttatttgt ttcctttgat cgttacttgt tttgcaattt
    71161 gtcgcatctc atccattttg cccatgtagt aatcgatccc ttgatcatca ccagcacgag
    71221 cagcatctgc acgcttctgg ttatagaggc tcatcagttt tttataccca tctccgatcg
    71281 tctgtttatt ttccaaactc ttttctaaat attggatggc ttgatcgttc acatacaatt
    71341 cagaaaattt aagtccaact tgttcataaa gtttcgctaa tttatgctta tcttgggtag
    71401 tggaaatctc ttgtttcaat tgtgtaatct ctgattgaat cttttcggtc gcttctggcg
    71461 atagggcttt ttcttctttt gccagaactt ttttcggttc tttttctttt ttcttaaaaa
    71521 atccaaacat cattttccct actttcaatt aaaatgcttt caaaactggg cttaagatcc
    71581 aggcatataa gagtgctgcg gctactgtat ctacatcggt tgctgtagca ttgatgaatc
    71641 ccatcgaatt aaagatcgtt actagcaaag cgggcaataa tgtaataaag aaaccatgag
    71701 caataccgcc aatgatcgct cctcggcgtc cgccaactgc gttgccaaat atccctgctg
    71761 tccctccggc aaagaaattg gttagcatac caggtaaaat catcgctaag ccgaacatgg
    71821 gcaaagtaaa catggcaata atcgtaccaa tcgttgtcgt aatgaaacct aaaatgacag
    71881 catttgggct ataaggaaag aaaactggac aatctaaggc agggatcgca tcaggaacta
    71941 atttcatagc tatccctcga aatgccggca cgatttcacc taacagtaga cgtacaccag
    72001 ctagcaatac atacacaccc actacgaatt ggatggcttg taaaaaggca aacatgatat
    72061 agttttgatc tcctgataat tcagaagcga atggttcgcc tgcaaataaa acagtgatga
    72121 tatatagcgg gaccatcacg accatgacag acaaataagt atcttgcaga aattcgaaat
    72181 gtgtcggcaa cttgatatct tcgatcgact ttttattttc gcctttctca ccaaaaagct
    72241 tagcaacacc ggcttcaaat aagtacccga tcgtacaaaa atgacctaaa gcaatatcat
    72301 ttgatccagt gattttacga atgatcggtt gcgctactgc cggcatagca atggcgaaac
    72361 aagctccgat aaaatcaccg accaagatca atacgatccc tcgcagaccg gcaaaatagc
    72421 caaagactgt cgtcatcgtt gccatccata aaattgcttg accagttaaa aaaatgtact
    72481 tccattttgt gaaacgagcc aaaataatat tgaatacaaa aatggctaag aacgtcagtg
    72541 caatatctct acctaaaccc aaatcattca ttgcttgacc attgatagat tcaatagaag
    72601 ggatgatccc ttgcatatga aatccttcag taaagatttt cccgaaataa gttaaacttc
    72661 caacgatgat gctcgaaccg gcactcaaca cttgaaatcc taggagtgtc tttaacgtgc
    72721 cagagatgac ttgtcctgct gatttcttct gcagtagtag acccaacata gcaatcaatg
    72781 caatggtgat cgatgcctga gtcaaaatat tttcgatgat aaaattgata acgcccatgt
    72841 ttttaccctc ttttcttttt gtatttagat aactccctta tcttgcagaa ctggggtgat
    72901 tttttcttca atttcatttt ttgaaacaat atttttcaaa tagatcatcg ccgttttagg
    72961 atcaactgcg aacttttcga actgcgaacg aaaattttct gctgtgatga tcacatctgg
    73021 tttcatggaa actgcggaag aaatatcaca atggtccagt ttggcttcta ctcctaattt
    73081 gttcaaaaca tcctcagcag acatctgtgc tgcaaaacta cttcctaaac ctgcaccgca
    73141 tacgaataaa atgttcaatt tgttcatctt tgttctcccc cgtatcattt attatttctc
    73201 aactactgaa aaaagcagtt gtttgaaatc ttcgatattt gtgcattcaa tcaatcgatc
    73261 caacttctct tcgttgttga tcaattcaat tagttctttc atgatgttca gatgcgagta
    73321 ggagtctact gctgccaaac aaaaaataat ctttactgga tccatttcag gattaccgaa
    73381 atcgattggt tgctcaatcg tagctacgct gactcctaat ttattgaccc catcttctgg
    73441 tcgggcatgg gctagtgcca tgtgtttgcc aatcacgata tacggtccgt aatcattcac
    73501 tgcacgaatc atcgcttcga catagcgctc ttcgacaatg ttttctttaa ccaatggtct
    73561 agcagctagt tcaatcgatt cctcccaagt agtggctttt tctttgatca tgatattgct
    73621 gtccgtcaaa atatctttta acataggctg gatctccctt ttgttgattt ctaaattgtt
    73681 tgtttcgaat aatttttcta acttgccata gatgattcga tctactcggc caccactttc
    73741 tttcatgaca tctattattt gagtgaataa ctccgtcgat tcatgactgt taggctctaa
    73801 ccgctgtagt tcttcatttc ttttcaaaaa gtcttctata accaagatat tcgtctcagt
    73861 taaaatcgga tcgacaacta aaagcggctt atttcgataa ttcaattgaa aggtgctgaa
    73921 aaccagattg acatccaatt tatctactaa ttcgatttct tttgaactta aaactgcagt
    73981 aatctcgatc tgaggaaact tttctttcag attctctgct aacagatttc cagtagccat
    74041 cccatggtta cagacaacga ctgatttata aacataattc aagtctcgct tgattacact
    74101 ggcgatcgtc gaaaagtgga tgacaagaaa agctatctcg tcctccagta tcttgccacc
    74161 aattacttct tcgatcgtag gaacaaaatg agtgactgcg cggtagattg ggaaataatt
    74221 tgttcggatg ttttctttca atggattcat gacttggata tgattcttga ttcttacaat
    74281 cagtgcagcc atgtgcttat agagattttc gaataaagct tcttccttta gatggaaagg
    74341 tacgtgtgtc tcgttctcta caaattggat caattggatc gttagtaatt gagcttgtac
    74401 ccactcgatt gaattggaga tatctctagc attgaacgtc tcaatagtga aacgaagata
    74461 atttctttct acttgcggga cattgtccaa ttgaaattcc tcgatcatgt ggtcaataaa
    74521 tgcagaaaag ctactgtctt ttatttctgt atttttccaa ttaatggcag agataaattc
    74581 atgtttcctg attcgctgga tccagatcaa tgtaaaaagc aggatttgtt ttttataaat
    74641 atcatcttct tgtttcaaca acttattgga atagatctcc tgtatttttt tgatttccac
    74701 caaaggaaaa taatggagaa agattttttg agccggtgtt aattgtgcat cttgattcat
    74761 aaagaagtcg gtcattccca gattctgatt gataaaatca tagatcatcg tacggataga
    74821 tcgttctgaa cctttataaa ccagtccttg ctttccataa ctgacgatct ctatcccata
    74881 actgaccagt gttgctcgta accgtctcat atcttcatct aatgtacttt tagagatttg
    74941 aaattcttct tcttttttgt ttagatggat cgggattcgg ctgtaagcaa cggctaaaac
    75001 tagatcaaaa atacgttcgc gccgattcaa aaagccgtct ttcacatctt ttatttcagt
    75061 ttctaatcga gcttgctttt ccttttccag cttcaacaaa aaacctttgt tgcgaattgt
    75121 acggatctcc ggaagctttt tactgctcaa ccagatattc gcttcaagaa cttcctgacg
    75181 gatcgtccgc tgactgacat tgaactcact tgctagatgt tttgctttta ctggatattg
    75241 ggctgtcatg attctgtgaa gtatttcaat tgttcttgtt ttcatagaaa tgatctcctt
    75301 ctgatttcaa gtatatggtt ccgcttacat aataaaaagt agatatttct gccacttgaa
    75361 gtggaaagaa agtaagcaaa gcaaacaaaa aataagccat atgctttttc atctcctcaa
    75421 cgaaaaagac atacgcctat tattacaaaa attttgccca tctatacaaa aaagaggttg
    75481 tgaaaaagct gtcctgcatc aagtaataag aacaactaaa ctaaaatagt gcttcctatt
    75541 tttcgtttgg ttgttcttat tatcgcagat gcaagctttt aaacaccatt tagtggagag
    75601 gttgtgacag agatgttcag ccttgagcga taagaagcga ttttgaaaaa tcgcttccca
    75661 tattttacaa aatcgtgcct tattgccgat aggctatctc tggcacaccg tttattcaga
    75721 aaaaggagct gcgacaaggc ttttgtcaca actccgttca taaatatttc ctgtcaatcc
    75781 agcataatcg gctgaataaa atcggcttgc catctttttt ctttctcaga aaaatcaaaa
    75841 taccatatct gagaaaaata ctgtcgttgt gtcttacttc ctgagatcaa tcgcccatct
    75901 tttcgatcga ctgaataatc acagcatctg aaatcaattc ggatggcaaa actattttcg
    75961 tggatctgtc gataatttcc tacttcctga atagttattt ttttagtatg atttcttaca
    76021 ccgtctgctt tgtttttttg cagaactttc tgatgttcgg caaacagctt gtctgtataa
    76081 cagcttctgg ctttttcagg agtttcttct tcccaagcgg attggatctt catgaatatc
    76141 ttttggattt ccagaaactg tgctttcttt tctttagccg ttcctgacat gcgggacaat
    76201 atctccgctt tcagcctttt cttttgtctt ttattcttga cgatagaaaa agcagaaacc
    76261 cctcctactg acaaaacagc taatgcaaga aatcccgttt gctcataact agaaggacct
    76321 gtacctggac cataataccc ataagaatct ctgccgtaag aacgactgcc aaaataactg
    76381 cccccatatg aacgaccacc aaatgaacct ctgcccgaac ttcgaccgct agatgtacct
    76441 ccaccatgac cacccgcgac agcgaatcct tgggtcggca gagcaaacaa acataggccc
    76501 actaacaaaa tgattgctaa ataattgatt tttttcattg tcttcctcgt tatcttttat
    76561 tacaaaaaac tatttttatg atagccttgc taaaaggaag agtcaataac cagatgcata
    76621 aactgttttt ttagcaattt tgtcttattt agtcattgag taacatcgga caaatgtatg
    76681 tgtcggcagt ccataattcc tctatcttca gcatgaacaa gcttgcagac aagagatctg
    76741 cgcttcctcc aggagagatt tggttttcct gacaaaagtg caacagtgct gtaaatggac
    76801 gatttggtaa agcactttga ctagcagcat gagcaagttt ttgcatttcc actaacgttt
    76861 tctgatcact gcgatgcaat acgcatgtgt catctagcga actcatcaaa actaacaaca
    76921 tatgcatttg tttctgtaca tatgaatcat tatctagctc cttagctgct tgaattgcaa
    76981 gcctgatatg cggataccct aatatcgctt cttcatatgc accatttacg ccatattttt
    77041 gcttcaccct atgaccatga gtggtttttt tatgacgata ttttgtatca ggaaaagcag
    77101 caagcttgct gacatcatca agtattttcg ataaagaaaa aggctgttcc atactgattt
    77161 tttgtgcaat gacactgatc aatagcccca ttgcccaaat cgctccttta tgggtattga
    77221 tgccatttgt agcaatcagc attgcctttt ccgcttttcg cccaatctca gcaattcttt
    77281 ctcttaactc ctgatcaata gcatgatgcc atgctatttg agccatttct tcaaaaaaag
    77341 gagttagcgt cttagcagac ttaagaaata aagcatagtc catatcggaa tgtgcacccg
    77401 catccagtgc atccactaat ccaggttttg gacttaatgt tacttctgcg atcaatgctt
    77461 cctctaccaa cgcagcgagt tgctgagtca ctgctttttt tcgttcgtca tttatcatga
    77521 cactcactcc ttcttcctac tattacttgc tgaaaccttt tttgatagct acccaaatga
    77581 tatgaagtaa ccagcttaaa gcaaatgcac aacttgccgt cattagataa gctacaagaa
    77641 tcatgactcc tgaaggtaac tgcagccaac tatctttaaa caataataac agtacttgga
    77701 aaacaaatac attggctaaa tacgtacgat aagcataaac agacaaccat ttgaacaatg
    77761 gcaattttgg cgaatcgttt acaatcattt tggaagcgcc tatgaagaca gcaaagatga
    77821 tgactaaact gtataaactt tggatcgtat tcaagtaagg tgcattcgca aagctcaagt
    77881 ctcctggatg tgcgagcaat gaacggacag aaaagaacgc gataactaag gcaacaggta
    77941 agaaaagata acgtactctg tccaaatatt gatagacttt ttcgtgataa acaaatgcgg
    78001 ctaccccata aatcatgaaa ctagcaacaa accgatctaa taagtaccag ctgtcatgat
    78061 aaggcccagt caatacttgt gtttgataga acagatacca tgcgacatat aaaatcgttg
    78121 aaccaatcaa tactggccat acatattttt tattagcaaa tactttgtaa ccgagccaaa
    78181 caaaaaacgg catgaacaat tggatctgca gcatcataac ggtataccat aaatgaggcg
    78241 ctccatcacc tgtgacatat ttcagtaaaa agcttgcagt atccgtatac ggagtttgtt
    78301 gttggactgc tggaaatagc caaagataag ccgtcgtcca tagaagatac ggaacgacta
    78361 gttcaaaaaa cttttctttc aaaaattccc aataagacaa atgctgactt gtttttacca
    78421 tattataaac aattgcaaaa ataaacattg gtgctgtgta tttaacgatg acgtaaaagg
    78481 taccaagaaa agctgtattt gttgcgctgt ggctgtttgc caaagcaaag ctcaatatcg
    78541 tttgcatcat aacagccgtt acagctgata ctttctggaa gtcacttacg ttgattttga
    78601 tttttttgtt ttcaaacatc tctttctctc tctttttctt tattaccaaa gttctttggt
    78661 taatttaggt ccttcgactg ttttcaatag aatttttttc tctggctctc tcaaatactc
    78721 ttttagtgaa aaaccgcctt gccgagtcac tacttgggta tcgaattgct caaaaaaagc
    78781 aggatgcgat tgaattacct gaattggcaa ttcaatcggt gaatttgcgt acagcagcaa
    78841 atcaaaatca ctggtttctt ttactgccgg aatcccagtc gataattcaa atcctaagct
    78901 gccgccgatt ccccatttac atcctgataa tatgtttcta gcagcatgaa attgctgata
    78961 aacaggataa tccatgattt tctcttgtct atacgattct tgttcgatga tgtcccacgg
    79021 atgaatgact tgtttgacgg catgttttga gatttctgtt gaccatctct gattgcgggc
    79081 aggtccacgt aaaccaatcg gtagtatttc tgcgtgatcc gtcgacgcac gacgaactgt
    79141 tccgaacatc ttttcagtta gaacgaccca ttcaggcaag gtgaaccctt ctatttcatg
    79201 tttatcaaaa aggacaatat catgtgcttg aatcattttt tatgcatccc attcttcgtc
    79261 cattttggca cggactttat ttgtttcgac acgtccgaat gttttggctg tttcgtttgt
    79321 gtatcgattt gtcagcatcg tgtcatttgt attgcggaca tcttgaatgg cttcattgat
    79381 tgcttcgatg actttgctga cattttgttc tgaagcagct tggtcggtga tatcttctag
    79441 gaaatgatag atcgctccta gttttgtata attattgata tcgtaagcga tagctggaac
    79501 tttttcagca gctgcttcga tttcagcaac cgaacgtttt gtcacacgtg cagcactttc
    79561 tttagacatc gcttggacgg taatttcttt attgtttaaa gcaatcaagc ggcttgattg
    79621 taatccatgt gctaaaaatg cacctgaaat agcatttcct gcaatcaatc caataactgg
    79681 atgtccagct tgacggagtt ttgcataagc agcagcacta ttagccaaag ccacatggat
    79741 accgatcaat tcttcgttat agccgtatgc ttggcttggt acatcgatca caacgatgat
    79801 tggccgtttc acttttttgt ctttatcttc ttcgtaaacg tgatccaaaa tttgtcccat
    79861 gcggaaacct tcaactaaac caacttcacc gtttcgtaca cggtacatcg ggtttttagg
    79921 atcaggtacg attgatgtca cgatggcttc ttctatgaat ttacttgatt taccataata
    79981 cacagtacta atagaagaag ttggattttt catgccagtt agtgccgcaa accaatcgta
    80041 accaacactt gctgcagctg gttcatttcc ttctgctact tctggtaagt caattgattt
    80101 tgcttgatgg ttagcaattg cttcattgta atcgtcaatg gccattggtt ttgaaagatc
    80161 taaagaatca agtaatgata aataaaatgc tgagctttca ctgcgatgcg tgtcttttcg
    80221 ttcgtccatt gctgcgatga cagcagcttt gattgcttct acactatcag tcactaattc
    80281 atcaatgatt tttgattcta cccgttgacg agatcctaat gtattccaaa tcaagtcttt
    80341 gtcactagaa tcaaattctc gaacgcctgc ttcttgttca ataacttctg gtccattcaa
    80401 accgactctc gctttttttg ttgcgattaa ataactcatt aatgctgaag taatcgacat
    80461 accgccaaat gatcctacac gtcctggaat caaaccgatg actggtacat attgttttag
    80521 agcaatgatt acatttccga tttctgaaat agataacagt ccgtagtttg cttcttgcaa
    80581 acgaacacca cctgtatcta gtacaatgac tggaaagatc tcatttcctt ttttattttc
    80641 ttctaacaca tgttcaagtg cagcaacgat ttttgcaccg gaaacttcac cgataccgcc
    80701 accttggaat tttccttcca tagcgattac tactgcttgt ttgccattga tcgtgccacg
    80761 gctgacaacg atcccgtcat cactttcagg gacgattcct tgtgctggta aacttggcgc
    80821 gatgatttga tcgaatggat caatcaattc acgtccattg tcaagtagag cgttcactcg
    80881 ttctctggcg tttaattcta caaaactatt tttcatctga aagttcctcc attgcttgcg
    80941 tcaatcgtag gtacacaact cctggtgtag caccgaagtc attgatcacg atatctgctg
    81001 aaattggata acgatcaaaa aatctcgtta atacattgtt ccatacttcg ccgaagccat
    81061 cgcttcctgt acaaattttt acagtagatt ctgttttttc tgttggaaac atcaagattt
    81121 ccaaatctcc tgaaccgacc actcctacat ggattttctt attgatcggc tgtttcgttg
    81181 gatatttata agctaattgt tccatggatc tctccccttt ttgtaaaaca gagcatacac
    81241 gttctgctta ttcgtctttc ttcttccatc tgggtaaatg gcgacaaaag gtttttctcg
    81301 ccaattttat gaccaagttc tgaatttcat tggtggttca tacaggccat ctgaccattc
    81361 gatcaaatca tcgatggttt gtgcagctaa taatgaacgt ttcgcttcac tacgtttgat
    81421 gcctagatct tctggtaatt tcacgatgcc ggctgcacgc aagctatcta gtgattcttt
    81481 cgtgctagtc attccaagag gtgtaacacc gccaattgct gcaattgctg cttgtcgttc
    81541 ttcttttgtt tttgctttgt acaaataagc aattccttct tctgtcacga tatgtgtagt
    81601 atcttcgcta taaatcatca ctggtgcatt ggctaagcct gcttgttttt gaacagctag
    81661 tgcatctaat tcttcgacaa agactggttt tttatttgaa ccatatgttt caaccatttg
    81721 aacaactaat ttttggcctt tccctaatgg atcatcattg tcgcgaagtg attgccatgc
    81781 tggtgtagaa tgacgacgac cgccaggatt atgtcccatg ttaggcgcac cgccaaaacc
    81841 agataaacgt ccttttgtta cggtagaaga gtttccttcg taatctaatt gcaaggttga
    81901 accgataaac atatcgatgg catattgtcc tgcagcttgt gataatgttc ggttactgcg
    81961 catcgtgccg tctttaccga caaaaaagac atctgggcga gcagcaatat atttttccat
    82021 accaacttcg ccaccaaaac tatggatgct ttcaacccaa cctgattcga tggctggaat
    82081 catcgttgga tgtgggttca atacccagtt cttcgcgatt ttccctttta accctagtga
    82141 ttcaccataa gttggcagta acaattcgat agctgctgta ttgaatccaa taccgtgatt
    82201 caaagattgt acgccgtgtt ctgcgtagat tcctttgatt gccatcattg ccattagaat
    82261 ttgcaattct gtgatgtttt gaggatctcg tgtgaataaa gcttctaatt gataaggttt
    82321 atctgctttc acgacaacgt ctacccagtc accagggata tctacacgtg gtaaagaatc
    82381 gacaacttcg ttgacttgaa cgatcacgat accatctttg aaagctgcag cttcgatgat
    82441 tgttggtgtt tcttccgtat tgaaacctgt gtacaagttg ccgtctttat ctgctttatc
    82501 tgcagctact aaaacaacat ttggaatcaa atcgatgaat aagcgtccat aaagttccaa
    82561 ataagtatgg atatcaccta atttcattgt gccgtcttca atcatttggg cgacacgcaa
    82621 gctttgtgca ccagcataag agaaatcgat cttgcttgcg attccttttt caaaaatatt
    82681 caaatgttct ggtcgtgaga tactagacat gatcaaatgc agatcattca ctttttctgc
    82741 atctactttt tcaagacttt ctgaaagaaa acttgcttgt ttttgattgt cgccttctaa
    82801 tacaactttg tcgccgggag caatcagttt ttccattact tctacgattt tttcagtttc
    82861 tacatacttg ccattgagca agttgctgat tttttccaat cgctgcgctt ttttttcgcg
    82921 tttagaagcc cagttgcggt ttggtttttc cataattagt catctccatt tttgatatag
    82981 aaacgtggcg tcatcaatac catgttggtc tagattgatc gtacggatcc ctttatctac
    83041 accaaatttt gtatgaatca gctttgtcaa agtattgccg gggggaaatt caatcgtcag
    83101 atccattcct aattcttttg ccaagtccat catttggttc cactgcacag ggaaagctag
    83161 gttttccaac aaatctttac gaatctcttc tgcgtcctga gtggctcttc catccgtatt
    83221 ttttatatac aaacatctag catcagatag atggacagac tgcatatacg gcttgaattt
    83281 cttgaccgta tcattcatca agagacagtg agatggcaca gggactttca gcagcttcgc
    83341 tgtttgcgct tggtgccgtt tggcttctgc caacgtaacg gataaacctt cgatactccc
    83401 agataacgta tgttgcatcg gacaattttc atttgacaga taaactggat ggtcaggatc
    83461 aaatgttttt gctacaattt cctcagcttc gcttctcgtc acacctacta ctacgcccat
    83521 tgcataaccg cttggataag cttctttcat taagactgcg cggtgataga ctagttccgc
    83581 tgcattctca aatctcaaag atcgacaagc aacagctgca caaaaagcac caatcgaatg
    83641 tcctgctgca atatcaggct gaataccgtt tttttccatt tcatctaaat aaaatactgc
    83701 ttttgtcagt aatgctagct ggataaaaac cgtatcttga tagttttctt ctttatcaac
    83761 taaccgatac cccgtcacat tttctaaacg ttccatgtac atttcaggga tatccgctaa
    83821 catccctaac ttctgactgc cttgtccagg aaataagaaa cctgtactca tgagcctcgc
    83881 ctcctcagca cttacttttt ccactgctat cttagtcaat ttctgctata ataaaaaatg
    83941 ccaaaaaaag aaaaaaccat aacaaaaagg tatggatggg atggataatt atgaaattgc
    84001 ttcatctgca gtattttaaa aaagtattcg aaacaaaaaa tgttacccaa gcagccaaag
    84061 aattatttat ttctcaacct gccttgagcc gtgcgatcaa acatttggaa aacgaattag
    84121 gtgtcccttt gttttaccat agcggcagaa acattgagac gaccgtttat gcagaagaat
    84181 tttatccata tgcagtcaag acattagaca cattagaaga aggaatcaaa ttattgtctt
    84241 cggtcaatga gaagatcgtt acttccgtca tattgtattt agaggtagcc tctgtttcta
    84301 ttccgaactt ggtccgaatt tttatggaaa agcatcctga tatccaatta acgatcatgc
    84361 aacatcattt accagatgat attaccgatc ctgtactgta catcacttcg gagatgaagc
    84421 ctggcatgac caatattcct atcatgaaag aacctgttta tgtcgccatt cctaagaaac
    84481 atcctcttgc taaaaaagaa catctgcaat tagaagatat ccaacgcacc cccatcttga
    84541 tgctgtcaaa gagcaacgcc ttccgtcata cactggatgc agcacttgaa gcgaaaagta
    84601 tcgaattgac gatcagttcg acgacagatg atcctgcgac attacgttct attgtcaagc
    84661 aaggattagc tgttagcttt ttcccaaaag tttcttggtc ttacgataaa aatgatcctt
    84721 tcgtcttgcg ggaaatcaaa gatttgccct tgacacgaac aatttattta tcttctagct
    84781 tcacagaaga tcatcctttg atcaaaatga ttgcaaaaac gatgcgggaa ttttatttag
    84841 gaaaataaaa aaaaaagagc tgggacaaaa ttcttgtcgc agctcttttt tcgaacaaac
    84901 ggtgttccag aagtaatttc ttagaaaata agccgaaatt tcccaaaaat ttgagaagca
    84961 attttcgtaa attccttctt atttcttgaa gctaaacatt tctgtcacaa cctcttctct
    85021 attgtcctag acattcattg cgttgattta aaacttccat caatcaaatc tgctagttca
    85081 tctagctttt ctattttacg tagatcaata gagatccaat gttctttatt catgtgatag
    85141 gctggaaaat acccctcttt tcctcgcaaa attccgccaa gttcaggatc gaccttcaaa
    85201 tcaagaatat tgatgatcct atggtcactt gcatcatcat agattttttc ttctggaaca
    85261 gacatcagga ccccaaacca ttttttgctt ttttgatgtc tgaatacgca ataatcagga
    85321 aattttttaa aaatatattc tggagacaaa ttgtatgttt cattgatgtg atcgattagt
    85381 tcttttttcg ttagcatctg cttccatctc tttcttattg attcgcttta ttcaaagata
    85441 ccacgtaaga atagaaaaat gaatacaagt agttgctaat caaatacttc gatatttttc
    85501 ttttttatca actcttaaat gttcaatccg cctgtgttgg tcttgctacc atctactatt
    85561 aaaatgttta ttattgtttg cccacctatt ttttgatttt agtggtatca tttttcaagt
    85621 tcatgatgaa attcttatca tcagtaaatg ctccagaaaa ataaacaatg attgaaaaat
    85681 cagttgaaac ttgaccaccg tttattttct ccgtattctt agaatagaag ggagtctgat
    85741 tgatgtattc actaatgaaa aaaatcatta ccgaaaaaga tattcgacgt catgtttctt
    85801 tagtagaaca attgctgaat catacaaaga taaccgtaaa tgaattagct gaaatcatag
    85861 ggacaaccga acgaacgatt ttttctgacc ttcaatctat tcgctctcag ctgcccgaag
    85921 gctgggacat catcagcgat caagcaggaa tcagtttgca aaaccagcaa aatttattaa
    85981 caaatgacct ttgggagatt tttttcaaac aatcagtcag tgtcgaactg ctgaaaaatc
    86041 tgctttttac aaaaaaagtt gctgtccctg attttctagc cgaccatggg ctttcttatg
    86101 ggacattgaa aagacatgtg actaaaatca atcagcgttt agcaagttac gacttgcaga
    86161 tcgatttgac gaaatataca gcttgtattc ttggaaaaga gcgtgcgatt cgtacttttt
    86221 atcatcgatt gctgattcct ttcactcata acaactattt tttcgaggat tattcgatcc
    86281 atgaatccca ttattttcaa tttttaagaa acctcagtca aactgaacta gcggttgaaa
    86341 cagaagagat tttcggaaca tgttggttct ttatcaatac gatccgtata aaagcgaatt
    86401 gccgattaga ttcttctatc cataccaaca gcacactctc ctccctgtat gactctgcat
    86461 tgaaaaaatt gtatttaacg gaaggtattt atcttaaaga cactgagctt tcttttgctt
    86521 ctttctgctt tctggaaagt tggaattaca acaataacta tggacaagaa accgctcgct
    86581 gcctgcatca ttctccattt ttagaggtcc tcgaaacgtt tgtagaggaa ctagcttcag
    86641 agctgtctct tgatcaacta aaaaaaacat ctctgactga taacttggct ctcctcatat
    86701 taaaatatca tgaatcgccg atattgattg aagcattgga ccgtcaatac catcgttttt
    86761 tagaaacata tgatcagcaa tatgcacact tgtatcaaca gaaagagaat ttactgaacg
    86821 atctaaaaaa agaaatagaa atcaatgaac caaattactt tctccaactc ttgtctctac
    86881 tgatccaaca aacgattttt tctatcaaac cgaatttgtt caatgtctat tttattttcc
    86941 aaggagaacc ttcatggaag gcgttcttgc agcaggaatt aaaagattac ttaggaaaac
    87001 gtgtctgctt cttacctatc gaactaaccg atctttccac tatttctttt gaaaaaacag
    87061 atatcttgat ttccaatttc cctctagatt ctctagatat ccctattgta tacatttctt
    87121 ctattccaac gaaaaatgag ctcaatcgtt tgactgaact cactttcaaa acttttttat
    87181 agtaacggct agcgactgta ttctctagct gttttttctt ttttgcgaaa cagtttgttc
    87241 atgatccctt tttctatttc tacaactaag aatagaagaa tcacatttgc tccaatcaat
    87301 aactgttgtg tcatcgttaa acttactgta ccgatcagct gctggccgac tggtagaaaa
    87361 ataaccatcc cttgcaataa agcaaggata cctaatgaaa gaaaaagcgc tttgttttgc
    87421 aaaagacctt ggttcaatga cggcttggac aactctctgc aattgatcat gtaaacagct
    87481 tgagctaaga cgatattttg cagaagaatg gtttgctgta agctttgccc ttcaaactgc
    87541 atagccaata aataggcagg aaccatgatc agtagggaga catagatgat ccgaaacaaa
    87601 tgataaccag ataggattcc ttgctttgga tctcttggtg ggcgcttcat gatttctgga
    87661 tctggcgact cgaaccctag tgcataagat aaagtgattg tcgttaccat attgacccat
    87721 aaaatctgta ctggggacaa aggtagcggg cgattcagca taagtgccca aatcaagatc
    87781 aatccttgag ctaatgcagt tggcaagaag aaattgatgg ttttttgcaa attatccaaa
    87841 atccgccggc cctcttttac ggcttttgca atcgtatgga aattatcatc tcctaaaacc
    87901 atatcagctg cctgtttgct tacttcacta cctttgattc ccatagcaat tcctacatcg
    87961 gatcttttca aagcaggtgc atcatttacg ccgtctccgg tcattccgac gatttctcca
    88021 tttttctgca aagctttaac aattcgcagt ttatgttctg gtgtcgttcg agcaaagaca
    88081 tcgactattg gcacttgctg aatcaactct tcatcagaca taagatccaa atcgatccct
    88141 tctaaaactt tggcagtatg ttttaagcca atttgttccg caatcgcttt tgctgtgtct
    88201 ttgtgatccc ctgtaatcat tttcactgag atccctgctt gttggcattc ctttacagct
    88261 ttgactgcac tttcttttgg cggatcgatc aatccggcga tccctgcaaa agttaattgg
    88321 gttaaagtct catgcgtgag ttcctgtttc gaatctactt ttttataagc aaatcctaaa
    88381 acacgctgac cttttctcgc aaattctgca gctgtctgac tccatgcttc ttgttctgca
    88441 gaagaaagag ttgatttagc aaataatact tctggtgcac ctttgacgta aatcaccgct
    88501 cctttttcct ccgcatgcat cgttgccata tatttgtatt cagaactgaa cggaatcttt
    88561 gcttctattt ttttcaaagg tagattggct gtttctgtgt gtcgaatcag cgcaacttct
    88621 gtcggattgc cacttaaatt ttctgttttc tgttcttctt ttgtttttaa ttcctgacag
    88681 tttttcataa tggacaacac gcaggcttct tcttttgcag ctacatccac aactgtcatt
    88741 tcattttttg tcaatgttcc tgtcttatct gaacagatca ctgtcataga acctaatgtt
    88801 tctaccgaag gaagtccttt gatgatcgcc ttttcttttg ccatttcatg tactccaacg
    88861 gatagaatca tggtcaaaac agctggcaat ccttcgggta ccattgacac caccaaagca
    88921 atcgtagccg aaagaaggat atgccaatcc ataccatatc gaaatgtcgt aaaaataact
    88981 aagaataggc tcaaacaaag aatgccttga aaaatttgtt tgtttaatcg gttgattttt
    89041 ttaatcagag gcgtcgtttg ttgttcgacc gattgcaatg cttgattgat tttaccgatt
    89101 tctgtcgtgt ttcctgtttc tacaacgaca ccgactgcag aaccagattg tacaagcgtt
    89161 cctgaaaaac ccatattttt ctggtcgccg atcgattctt cttctgtcaa aacggcatga
    89221 cttttttcag atgcttctga ttctcccgtc aaaatcgatt cttccactaa taaatcatgt
    89281 gcttcgatta atcggatatc tgctggaacg acatctccgg cacgtaagac aatgacatct
    89341 ccttttacca gttctttcgc agggattttt tcttgcatcc cgtttgatag tactatcgct
    89401 tcttgtccca tcagctgctt caaaccattc aatgattctt ccgcttttct ttcttgccag
    89461 taactaacga gtccattgac gatcactacg aataaaataa tacttccttc aatatattca
    89521 ctagaagcaa atttcaaaaa agaggcaatg atcaacacga tcatcagcag atcagtaaaa
    89581 tgctttgcta tttttttcca tcgttttgtc tgctgccttg cttttatttc attatatcct
    89641 tgatcagtca ggcgtttctg tcgttcttct tgcgacaagc cttctgttga tgtttgatag
    89701 tttttcaata catcttctac cgtcaatctg taccagttca tcctatcccc tcctgtttaa
    89761 ttgatactgt tattatacgc agggaagttt tttcaggcgt atcgattatt tcaggggggt
    89821 ctgaaataat tcgttttgca aaatgtatct gggcctaact gatatttcta ttgcttattc
    89881 ttttatatac aaaatttaag aatacaaacc agataaccaa taaaaaagga ataaagcgtg
    89941 tcgtgctttc gaataacaag ctgaaagcaa ttgaagtaaa aaacaccaac aaagcaagat
    90001 ctttatatct ttgtgtagtg ttccgtttct tttctttaat aaattgaaca tgtgcacaca
    90061 agatcgaaga ccacacaaat agaaaacaac tcgttgtcat gcctgccaaa agctgaaaaa
    90121 acaatcgact atcagctaca aaaaaatgta gacaaagacc ggcaaaaaat aacattcctg
    90181 taattaaaat accgtgtaaa ggtacgcctt tcttagaaag cgcacaaaac caatggggca
    90241 aacttttttc ttttgctgtt tgatataaaa tcctgctagt actgtagact gcactatttg
    90301 ctgaggacaa agaagcaacc aaaatcatta gattgacaat tgctgctccg ccttggcaat
    90361 ggatcacttg cagcatagtc actaaaggac tttcttttaa atctaaacaa tgccatggaa
    90421 caacaaataa aatcgacaaa atcgttccta tatagaaaaa gagaatacgc caaggcagac
    90481 agttgattgc ttttttcaaa ttcttttgcg gatcacttgt ctcgccaacg gttaagccga
    90541 tcatttcaat tccagcaaaa ctgaataaaa ccataggaaa tgagactacc attccagcaa
    90601 agccaaacgg tagtaattca tcgatctgtc caaaattttc tttgattccg atagactggg
    90661 tatgaaaatc tgagacactc aatcctatac ccaataagat aaaaaggaaa atccccaaga
    90721 tcttgatgat tgagaaaaaa gattccaatt caccaaacca cttgactgca acaaggttga
    90781 ctgccattaa gatcattagt gcgctacctc cgcttatcaa agctggtaca tgtacaaacc
    90841 agaaatggat atacaatcct attgctgtta attccgcaag tccagctcca agccagcaaa
    90901 gccaataagt ccaattgaca aaaaatgccc actttttgcc taaataatag tcaacgaaat
    90961 cactgataga gttaaatgta ctatttgcta aaaataaatc acccatggcc cgcatcaaac
    91021 cataaatagc caaaccgcat atcaaataaa cgagaataac agctggccct gctgatgcaa
    91081 ttgcctttgc tgaacctaaa aataatcctg ttccaattgc gccacctaat gcgattaact
    91141 ggatatttcg attagttaga gtccgtttca ttgccggttt ttccatcaag ttcacctttt
    91201 tctttttcaa gcagcatggc gacttcctgt tcccaagaag gtttctttat tttttcaata
    91261 cagagtggta ctacaaacaa aatgacactg atggctgtga ctgcaattag attgatccat
    91321 gttccagctt tcatcgtcag tccgacagaa aatccgattg ccgcaaataa aacaataacg
    91381 acagtccaaa ctccaagatt gctctttttc tctttttttc caattctaaa tggtcgattc
    91441 aactttgcat gtgtataacg caatctcaaa actgcgatcc ccatgatgac atagacgata
    91501 cagtagataa cagtagttga atttacgagc atcaaaaatg cttgattgac gccaggaatc
    91561 agcaaataaa aaagtgcaaa gattgagata atgacagact gagtcaaaat aacggatttg
    91621 gatacgtcaa attgattcgt cttccaaaaa ttgaattttg gtggatacgc accttttcta
    91681 gcactttctg tcactgtctt tgctggtcct gaggcccaag cagataattg gactgctaca
    91741 ccaatgaaga caagtaaact aaacagattc acaataagat gtggcaaacc aagaatttgg
    91801 caataaatcg aaatagattg cgcgatatta ttcaattcca tttgtccttt tggtacgaca
    91861 tttgctacaa ctaaagcatt caaaatattg acgaaaaata taaaaattaa cgcggcaaat
    91921 actccgcgaa tatacgtttt aactggattt tccaatcgag tgatatacac agaggacatt
    91981 tcgataccag taaaaataaa catgatcggc gcaaaataaa caaatgagga tgcagtcgta
    92041 gtatctggta ttaatttgtc ccatgaaaaa ctgcctaatg ttccggttgg tacgatccca
    92101 actttgatcc acgcagcaaa ccctaataag agcatcatcg tcaaaggcac gtacaatcct
    92161 aaccaaacac cgacttttcc gccgattttt gccatatcaa atttcaaatt caaaaaagtg
    92221 atgatccaat aaacgactaa aatgacaact aatgtaaatt tgctgttcaa accaagcggc
    92281 acattatcga taatattacc aaacaacgga gcgagtgctg aagcgaccat caccatccca
    92341 ggaaacattt ggacccataa cagccatgag acaacgaacc cccatttttt tcctagagca
    92401 ttcgataccc aaagttctgg accacctttt tggggaaaca ttccagcaaa ttcaccagag
    92461 atcaatgaga ttggaaaagc gtacaataat actgcaacca gcatgtaaaa aatcatcgtc
    92521 cagctcgttg ctgctacatc tggaatattt cgtacacttg ctacaagggc gcaggtcatt
    92581 ccgataaagg tcatggtcgt gatttgcttt gatgaaccag tatctttcat ctttattcct
    92641 tctttcaata gcgacatatg tttttgtttt cttttatttt tgttcaagaa cagaaatgac
    92701 aaggtttcat cgattcagta caaaacccag tatcaccgaa acatccattg aacgatctta
    92761 gtgaactggg tttgatacat gaaacgaacg atccaactta tagaacgttt tataaaaaag
    92821 aatatcttgg cgaggaatat atgaaaaaca gggggaaata caattgtttt gatgaagcaa
    92881 ataattgtct gttctccata tgtctcttgt tattctttca cacaaaaatc agttctattg
    92941 gatgatttcg tttagtttct tttccattgc tttggcgatt ttttttgtat aaaaattgaa
    93001 aattcgatca tcattcaaat aaggagtcat gatagccgca cgtagcagag tgattttctg
    93061 ttcttttttc cattcttctt tcgttagacc cattctttca acaaaacgaa tcggagaatc
    93121 accgtaatct tcttgtgtaa atgtagtatg agaagtaata aaccgttcac cataaacatc
    93181 gccatccata taagaagacc gatcgaacat tttttcattt aattcattga tagcgttcaa
    93241 atctgtacag ccttgttctt tgaataccca attgaccata ttaaaatcag gatgattcaa
    93301 tggatagact tcgattgttt ttcctttaac agtaaaagtc agatgatcga gaaattctct
    93361 gaatctttga gctgcctcga ttgaagcccc aatcaattga ccatatccgg tcacattcaa
    93421 tggcaataca cgatgtgctg tccagactgc ggcggcggtt gctccagctt ttgatccttc
    93481 caaaatataa gctcctaaca tatctggagc ctttactgat ttttcaaaca catatggcgc
    93541 gaaataagaa atgatatttc tcatattttt atgtttgatt acgattcctc cagcagcata
    93601 agggacatag cccatcttat gaggatctat cgtgacagaa tccgcttctg aaatagctct
    93661 gaacccttca tatacctctt tgtcaatcgt tacgcagtgg tggaaaacat gatgttcttc
    93721 aaagaattca gctaaggaag catacggcac aaattctcct gcttcgttta gaaacagtga
    93781 acgcgcatag ccgccatatg cggcatcaac atgtaaatag aaatagatcc cttcatcttt
    93841 taacctttct cttaattgga cgattttatc tacactgtct acttgacctt cttctgtcgt
    93901 tcctacgaca gcaaccacac ctagaattgg gattttttgt ccagctaatt cacgaattgt
    93961 tttttccaaa gcattaatat ccattcgata atcctcttga actgggatag caaccatctg
    94021 atctaagccg actccgcaaa tatctaacgc cttcatccaa gaatagtgtt ttgtttgagg
    94081 gaccagccat ttccctaatc tttggatatt ttttccgcta cgggaagaag ctgctttgac
    94141 ctcatccatt tcttcgtccg taaagctttc ggtcatttcc aaaatttctt caacagataa
    94201 attcaaaagt tcccactcag acattttttt cactttttca ggcagcactt ccttgacagc
    94261 taaaggaatc gatttgatgc aacgggcgta ccataatcct tcaagattag caatagaacc
    94321 atctgctgca atgtgtcccc atccgtctgt catattgaat aatgaagcaa aatcctgtcc
    94381 tacttctgct tccatttgcg aagttgccat agaagattcc aatgcaacat tatttgggtt
    94441 ccacagcatc gcatagttgt aagctaataa agcaggcatc aatgtttcag cattcatttg
    94501 tccccagtaa cggcctgctg aatgccatgg gatcgatcct gcgcgtaatc gctgactgac
    94561 ttcatccaag acctctatca tatggtcgcg ggtggctaga taatctggag tgagcttatc
    94621 tccttcgcta attgcaggca tatctgaagg aatataattt tctctccatc caagatgttc
    94681 atctaccatc ttgttcaaca acattttata aactggtcca ttttcagctt tgtcgcctat
    94741 aaagacggcc ttgatatcca tatctttcat ttttcttccc tccatatttt gttggtcaat
    94801 gtcttgcaga tacatgtgtt tatctgtaac acctcttcac tatagcgcca aaaatgagaa
    94861 tttttttttt ataacatcaa atccacgtag atttttacaa acaaaaataa ataaagtcgg
    94921 atttttctga taattcaacg caaaaaagcg aacaattttt caataaatca gtcttctaaa
    94981 atagataacc taaaaaaagc gaacaaacaa ataaaatgac tgtccttcat tcactaaaca
    95041 tatttttatt tttttatatt gttaaaaatt cgcaatttat tttttaatag aattatctgt
    95101 tttccaaatt ttataacata aaaaaccttt tttaacggca tttatatgct ttttcagaaa
    95161 ttaactgatg atttcaaaaa gaaaacatgt cataatattt cccatattgt gcaaaaaaat
    95221 acaaggcgga ggctctacta tgattgaaga atatattgag aaaaatattt taagacagct
    95281 gtttttatgt gggcaattct atgtaaataa agaagtcaat ctagaaaagc tctccaatct
    95341 cctgcatgtt tgcaaaacca ctttactcaa tgatatcaat aacataaaaa aagaatttga
    95401 agaacagatc gcttatacac atcgggaaaa ggaccgttac actttatatt tttcagaaca
    95461 tatacctcga tgcaagatca tgcagcaatt gtctcaaaat tcgttgtttt taaaaacttg
    95521 tctgctttat ttggaagaag atgaacctga ttatcttcaa ttaactgaat gtgaattcat
    95581 ctctgtcagc aaagcttatt cattaaaaaa acaagtatta gcttatttta atgactgcgg
    95641 gatcgaaatc gaccgctact cccctcgatt cactgaaatg gaaagacgtc tgctgcttct
    95701 taatgtgtcg tatcgtttag gcggttttaa ttcttgggag ctacctgaat cgttttttga
    95761 acgtgccgat cgtttcattg aatccgtaac ggaaaatagt ggcagatttt atgataaaga
    95821 aaataaagaa atcttatcga ttggttttgc tatcagtttc ctacgccagc aagtgtgtgc
    95881 tgtgacaata gattcaaaat ttatcgaaga aatcaaaaaa cgtcctattt acaattatgt
    95941 cgaatcagca tgggaaaaca ctgatttcca aacctattat aaaaaagaag aattcgcctt
    96001 tattttgaca ctattcaatc tttgtaatta tggtttccat tcttatcagt tgattgcgga
    96061 agactttcaa cagctacacc aagtgtttat tgacaatact cccgaaataa aagaactagt
    96121 tgctaccttt gaatctcact tcaatcaaga attattcgga aaccaaccgt tcgaacgtgc
    96181 gctgattcat ctgatgcgtt cggcttggga taactatcaa ttattcatgc cagaaaaatt
    96241 ttatctttta aatgaggaac agacaaattt atataaagaa gtccagacaa ttttttccag
    96301 ctggtccagc cagttacctt atgatctacg gttgaatcct aattgcatgc gggcttttgt
    96361 gatcgaattg agcggaattc tccgtctgac aaaagaacat ctgactatct atatcgtcac
    96421 aaattcggat gttcattatc tgatttatcg ggaagccttg gaagctgtaa ctactttcga
    96481 ttttcaagtt gcaccaacaa tttattcaag tatatctgat ataaagaagt atgcgcagca
    96541 gtcctcaaac cgggtattat gtgaacgtac attatataca ccagatgcgg tccaatatga
    96601 aaatattatt cctatttcga tcaatacgat cgatcgagct atcatttcag ctgtgcaaaa
    96661 caaataaggg gctgtgacaa aaatcatgtc acagcctctt atttgttgaa gatgcccatt
    96721 tctgtaacaa tctcttgata cacgtcaggc tcacaacctc acttattttg gacatctacg
    96781 ggtccgatct ctttatttaa aaaataagaa atgatggttc ggataactac caacgccgct
    96841 agttggaaaa tatcctgcaa ggtaggtttg ataatcgatt cgatgatccc agctgaaaca
    96901 agaatttcca aacttagaag tatatagcct cccaacatct ttttaatagc attgttctcg
    96961 ctgactcggt cgctttgcac ttttgtatgg acaaataaat atttcaaaaa atccttgatt
    97021 gatagacaaa caccccaaac caataaacag atagcaaaga tatcaagtcc cagtacaagt
    97081 ttttcgaaat atggaagaag aaactgcatc aactctgtcg tcatcctacc tctcctcttc
    97141 tttgtttttc tttgtcttct atttcctgat tcaagaaata agaaatgact gttcgaatga
    97201 taataataag tgcaagcttc aaaatatctg tccaagtcgg cttgataact gactcgatga
    97261 tatcagcaac gactagtact tccaagctta acaaaatata acttgctagc agttttcttg
    97321 tatctgcatt acgagtatta cgttctgcat agtttttctt gacatgagac caatcaaaaa
    97381 gtttgaatag aacaagaacg atcccgacaa ttaagatacc gatagaacaa atatttaaaa
    97441 tagcaatgat ccattctaga aattcaatta aatttgagcg aaacatatac gctacctact
    97501 ttcttttaat ttgttcctat tcttttattg taaaagaaag ttaccttgtt cgtctattta
    97561 aaagttgttc taatgataac ggttcgctta aatttttctt tttcactctc ccatttttct
    97621 ccctccatcc ataaatcgtg gaagtatcca aaacaacgta gcaagttaaa ataaggctta
    97681 gtccgtcatc agcttttcaa agaagcactg tctcggtgct ttttttgtac ccaaatttct
    97741 aaaaatagaa ccatacacct cctcaaatct tttcttctta cctaccctat ttttatttac
    97801 aggaaaatca gacacttctt cttttttgtc aaaatttcct ttttcttctc gtcctatcat
    97861 ttggcattct aataaaaacg aactatcctt ggatatagaa agagaaaaca aggaggcgtt
    97921 tttcatgagt aaaaaattaa tcaaagtcgg catcggttta ggtttgcttg cccttggcgc
    97981 tgcatatcta ggaaaaaaga caggactatt cgaagatgac agtcatttat atgatgaatt
    98041 cgaatcaatc tagcatccta tttagttgaa tcactacgga catacttgga agacattgga
    98101 ggaatcacaa tgagaaaaag taaagcaata atgattggtg ccggtattgc caatatggca
    98161 gcggctgttt atttgattca agaaggcaaa tggaaagggg atcagatcac cttttattca
    98221 ttggatgatc acggttcaaa tgatggtgca ccgaccgaca ctgtagtgga ttaatattgg
    98281 aataaaaacc atccattaga aaaccaaaaa ggctatattg ctcgaggcgg tcgcatgctt
    98341 aattaccgta cctatgtaga tttgatggat ctgttaagtc gaataccttc tgttactgag
    98401 ccagggatga ctgctgaaga agatacgaga gattttgatg caaaacaccg aacctttgac
    98461 aaagcacgtt tgatggaagg tggcaaagga atcattgatg gcggaaaact gggattcaat
    98521 aataaagacc gtattcttct gactaaactg attgctatgc ctgattcaga agaagaaaaa
    98581 ctagacaata tcactatcga agaatatttc aaagatgatc cacacttttt tgaaacaaac
    98641 ttctggttta tgtgggaaac aacatttgct ttcagaacac gtagttctgc tcaagaattg
    98701 cgtcgctata tgcatcaaat gatttatgaa ttcactcaaa tcgaacatct agtgggcgtc
    98761 aatcgaacac gttacaatca atacgaaagt attatgcttc ctttgatcga gtacttgaaa
    98821 gaacaaggat gcaaaatcat tttgaaccgc cgtgtcactg actgggaatt caaagaaaca
    98881 ccgatgcaag atgaaatcac tgtgactggt ttgaaaatta ccaatacact gactcaagaa
    98941 gaagaatacg ttgtggtcga tgaagataca gcagttattt tcactaatgg gtccattaca
    99001 gattcagcta ctttaggaga ttacgaaaca ccagctgtag aaaatatgga ttatggtgca
    99061 gcttctagtc tctggaaaaa agccagtgaa cgtttctaca acttgggaaa tcctgacaaa
    99121 ttctttgctg atcgagatgc aagcgaatgg gtcagcttta ccctgacgac gaaaaaccat
    99181 ttgttgttga atgaaatcac ccgtatcacg actcaagaac caggaaatgc cttgaattca
    99241 tttatttcaa ccacaccgat cacgccgtta ggccaaaaag atgtcaatat gtctatcgtc
    99301 gtgcatcatc aaccgcattt tacgtctcag aaaccaaatg aaacagttat ttggggttat
    99361 ttcctttatc caagacgacg tggcgaattt gtggataaac agtatattaa aatgaatgga
    99421 aaagaaatgc tagaagaact gattggacaa ctttcaaaag tagatcctgg accagtgaat
    99481 attcgtgaaa aagaaacaga aattttcgac agcgtaataa ataatatccc tgtctatatg
    99541 ccttatgctt ccgcgttgtt caataatcgt gccaaaagcg atcgaccaaa agtcattcct
    99601 aaacattcga ccaatctggc ctttacagga gaatttgtcg agcagcctta tcagatgatc
    99661 tttactgaac aaagtgctgt acgttctgga gaaatcgctg ctttccattt tgcgggtatt
    99721 cctatgtccc gtcttgtcaa aacaccgcgt tacgacaaag atattcctac tctagctcgt
    99781 gccgcaaaaa aaatgtttga ataatcaaga ggttgtgaaa aagctgccct gcatcaagta
    99841 ataagaacaa ccaggctaaa acagcttctt atgtttttga tttggttgga cttattaccg
    99901 cagatgcaag cttttgaaca ccgtttacca agaggttgtg aaaaagctgc cctgcatcaa
    99961 gtaataagaa cacccaagct aaaactgctt cttatgtttt tgacttggtt ggacttatta
   100021 ccgcagatgc aagcttttga acaccattta ccaagaggtt atgaaaaagc tgccctgcat
   100081 caagtaataa gagcagccaa gctaaaacag cttcttatgt ttttgacttg gttagactta
   100141 ttaccgcaga tgcaagcttt tgaacaccgt ttaccaagag gttatgaaaa aggcgtttag
   100201 tttcgagtat gaagaaaaat tttgtgaaaa tggttcctca cgtttttgca taaatttgac
   100261 ttaataccga gaaactgatc ccttctgtca taacctcttt ttatttatgt ctgtttcttt
   100321 ttgaaaacaa ctattttttc aacgtttact ctcttgccaa tccaacatca tctaacggat
   100381 acaactattt cgatgattac cattacacta aaaaatcgga aacacacctc tccgaacaca
   100441 aatacataag actatagaat gtttcattta agaaaaaaag cgtttcatta tcaaaaaggt
   100501 aagatctcct ttaaaatgaa attatctgta gtactaataa tgcagacaat tttatgaatc
   100561 tgaaaggagc atctccaatg aaagcagtca cttggcaagg aaaacagaaa atggaaatca
   100621 gaaacgtaga tgatccaaaa atattagaac caacagatgc aatcatccgt atcacagcaa
   100681 cagccatttg cggctccgat ctgcatcttt accatcatgg tgaagcagtt ttggaaaaag
   100741 ggtatgttgt aggacacgaa ccaatgggaa tcgttgaaga agtgggatct caagttcaaa
   100801 cattgaaaaa agggcaacgt gtggttatcc cttttaatat tagttgtggt cattgtcatt
   100861 tctgcctgaa tcacatggaa agccaatgtg atgaatcaaa tccagatcag ttatatggcg
   100921 ggcttttcgg atttggcaag ttgaacggta atcattgggg tggtcaagcg gaatatctaa
   100981 gagttcctta tgcagactct acttcatttg ttgtaccaga taatgatctt cctgatgaaa
   101041 aagttttatt tctatcagat attttgccca cagcttattg gagcgttgaa aacgcaggag
   101101 taaaaaaagg cgatacagtt gtgattctag gttctggtcc agtcggtcta ttcgctcaaa
   101161 aattcgctgt tatggctgga gcagagcgcg taatcgcagt agatccagta cagcaccgtc
   101221 tagataaagc ggctaattac aatggggttg aaacttattt cttagaagac acggcacaag
   101281 ctggaacaga attgtatgaa ctggctaaag gtggcgcaga tgtcattatc gattgtgtag
   101341 gaatggacgg attagagcct gtcaaagaaa aagcgaaaaa tttagtaagc ttacaaagcg
   101401 gcacaatctc tccattacag atggcatcgc aagctgtcaa aaaatttgga acagtccaag
   101461 taactggtgt ttatatgact ccagcttctt cttacccgct gcaagaattt tttatgcggg
   101521 atatcgctgt caagcatgga caggcaccaa ttatccatct aatgccaaaa atttacgcaa
   101581 tgattgcaga aggactattc gatcctacac aaatcattac tcattcgctg ccactatcag
   101641 aagcagcaca agcatatgag atctttgaca aaaaagaaga taaaaatata aaagttgttt
   101701 tgaaaccttg aattcagtaa aaaatacagg tgattttctt tatgcccaaa atcaaacgaa
   101761 atacccatga gatcgaaata ggaaaagaag aagatatcga aatacgagga cgtcgcttcc
   101821 gcttctattt ccaaaatcgt tacacattga tctcactggc tgtcgattta ttaaccggga
   101881 ttttctatat ttttggaagc cttgcttcct taactaaaat ccccgatcga tatggcacct
   101941 atttttattt ggctggtgct attttcttga cgattcgtcc tattctcaga atcgtaagga
   102001 acatttttat ttacgacgaa aaaaaagcaa tgaagagaac aagcaaaaaa aaagaggcat
   102061 gagaattttt caaatctcat gcctcttttt tcaccctatc gtatcacttt gatacagacg
   102121 agcggtcgtc ccactttctt ccttgaccat tgtcaaaaat gtccactcgt atcgttcata
   102181 aaacgtggta tgatctgtaa ttaagtaaag tttttcgtac ccaagttgat gcatttcttt
   102241 tctgatttcc gctaatatcc gcttggctat cccctgctta cgcttttccg gttcaacaaa
   102301 cagggcacaa agatttggtg tcagatcagg gcggttatgg aagtcgtttt caatgacgcc
   102361 cgctcctccg acgatggttt ctttttcatc taagaccaca taccattgag gaatactttc
   102421 cttattagcg attccagtac gaatactttc ctcatacaca acagcaggaa ctccccattt
   102481 tgatgaaaac caattcgcgc tttgttttac taattctggg tgttctctta atttgatcca
   102541 gttatatgta accaatgttt aaacctccga ctaaaataaa acgtttacaa aaattataca
   102601 agtctttatg aaaattagca atgacaacaa tagtgatgta aaaaaaaaaa cggaaaacaa
   102661 tcacttgttc tccgtttttt gattgatcaa gcatctaaag cttcttttct tctttcttgg
   102721 atcgtagcta ccttgacgaa cggtagatag atcaaaatag aaagaaccaa attgacagcg
   102781 gctagtacac cacctgcaat actttttgtt gccaatacac cgccaataat tggtggagtt
   102841 acccatggag gcataacggt agctgctggc actaaacctg tagaagtcgc aaagtaagca
   102901 acactaacta agaccattgg tgtcaaaata aatggaatga acatgatcgg gttcaacacg
   102961 ataggtaacc caaacatcgt tggttcatta atattgaagg acacctggcg caatcgacaa
   103021 gtttgttaca accatatatg gtttgttttt acgtcctact aaataaatag ctaacagtaa
   103081 acctaaagtt gcacctgttc ctcctaagtt gacaaagctg tcaaagaaag gtttgttcac
   103141 gataaacggt gcgctatgcc cagctgcgat tgcattaacg tttgcttcaa tggctggcgc
   103201 attgattgtt tgcatcaaag gatcgaccat attcgctccg tgtaaaccaa agaaccataa
   103261 gaatggtgtg ataaatgcaa gcagtaaggc agatggataa gagtttgcaa gtcccataaa
   103321 tggttcttgg accactgtat agaaagaacc tacgatatca tcaacaccga atgcagcaaa
   103381 aattgcagca actaaagcaa acgcagagat agtgatcatt gctggtagta atgaagcaaa
   103441 tgcttttgca actgccggtg gcacaccatc aggcattttg atgatcaatt tttcatttcc
   103501 gcttaaacgt acaaagattt ctgttgaaat aatcccaaca ataatagcaa taaacaaacc
   103561 gtttgagctc atacctaatg ctccgcctaa agcgaagaaa gagcctaaag aaatcacacc
   103621 tgcggaaaca ccgtcttttc ggtagccgtt agctaaatga taagcaagca agaaagcaat
   103681 caagacagaa agaatggcaa atgtaccgtt ccaagcagca gccccgaatc ctttccatga
   103741 ttcaccacca aaaattgagt tcatcaattc ttggaacccg ggaatcggta agttattgat
   103801 cattactgct aatgctccta agatcattaa cggcatactc accataaatg agtcccgaat
   103861 cgcaactaaa tgccgttgtg caccaatttt tgatgctaca ggtataaaat gtttctccat
   103921 aaatgcaaca aatttatcca taaatcttcc ctcttttctt tactgtagat tacgcgttca
   103981 atcgacgata cagatcaatg atctcttttg ctaaatctgt aaaagctata gatgtcatca
   104041 gatgatcttg actgtggacc gtcaaaagcg tgacttcaac gtgatttcct tgtgcttctt
   104101 gtgtcagcat ctctgtttga gagtgatgtg cttggactaa agattcctct gcatctttga
   104161 ttttttcatc tgctaaagtg aaatcacctt ttttcgcagc agcaatcgct tccattgcat
   104221 cgctttttgc atttccgcca aacatgatca gacccatgat ggcttctaga tttttttgat
   104281 cttccattta ttgtatcctc cgataatttt tattatttgt ttattagttt ttctgcttgg
   104341 tcgagtactt tttcaccatt catcatgcca tagtcagcca tattgattac atctaaagga
   104401 atgcctttag gtgctagctt ttcagcaaat tgattttgca taaagcgtac ttgcggtcct
   104461 aaaagtaaaa cgtctacatt tttagattct agttgattat ccgcatctga agcagataca
   104521 gcaaagatgt ctgcttccat tccttttgct tctgctgcct tttgcatttt tgtgactagg
   104581 agacttgtac tcattccagc tgaacataca agcatgattg ttttcttttc cattttaaac
   104641 acgttccttt ctatacttac acttgtatat aagcaagtag cgtgccaact ttaaaagcgc
   104701 tttaaaaagt tgatatcatc acatttaagg tctataccaa gttgcataca cactaaaaaa
   104761 gaaagcgcac actaaacaca caaattaatt tttattacct tttttctttt aacgtgcaca
   104821 aaaaaagttg tgacacaaaa cttgtcacaa cttttttcaa ataaacggta ttcaaaagct
   104881 tgcatctgcg gtaataagtc caaccaagtt aaaaacataa gatgttgttt ttactttgct
   104941 attcttatta cttgatgcag gacagctttt tcacaacctc tcaataaacg gtgttcaatc
   105001 agctgaccct gggtattagc tcaaaacgct ggattcacaa cctcttttat ttcaaataac
   105061 cagtaaatac aatgaacatc tacaaaaaac ttctatttat ttatagattt cgtcaggcaa
   105121 atccaattct ctcttatcaa tcaaagtctg ataccacgaa gcagattttt tcaatgttct
   105181 gcttcgttca tcgttcaatt cgattcgaac taatccatat cgattcttaa atgcattcat
   105241 tggtgacaca cagtcagtga atgcccataa catatagcct tcgcaatttg cgccttcttc
   105301 tgttgctttg atcaagtaat gcaaatgctg tccgataaat tcgatacggt aatcatcttg
   105361 cacgattcct ttctcatcca tatactgttc ttcatattct cttcccatac cgttttctgt
   105421 tatcagccaa gggatattat gataattttc ttttagatac atggctatat catacatgat
   105481 tttcggatag atttcccatc ccctggaatt attcattttc ttcccaggta aatcaaagac
   105541 ttcataatat cgttcaggat ggaaaggtgt ctcttcattc cattgataac gaggggcgcg
   105601 aacgcgttta ggataatatt gattgattcc aaggaaatct actgtatgat gctgaatcgt
   105661 ttctaaatcc tctgttgcag gatcgaaata aatcgattgt ttttgacaca actccaggaa
   105721 ctcttctgtg tagtatcctt ttaccatcgg atcaaaaaat actcgattat aaaacaaatc
   105781 atacatataa gcagcttttt ggtcttctgg agaattggat cgagcataga ccatttcagg
   105841 atttaaaata cagccaatcc ttccaccata atttccgctg cggtaaatct ctactgcttt
   105901 agccgttgct aaaactttat gataattcca catcatccac ttttttgtgt tttgttcatg
   105961 cggccagcga acagcatcca aatagcaacg cgtttgggga acgattggtt cgttgaatgt
   106021 aaaccagttt tttaccctat caccgtaacg atcaaaggca atttgagcat acttagcata
   106081 aaaatcaact acttttcttg aagaccagcc atcatatttt tccaaaagaa aagctgggac
   106141 ttcataatgt tccaaacaaa tcatcggttc tacgtctgct tcgatcaaag agtcgatcat
   106201 ttcacttata tgctgagcat attcttcatc tactatacag ttctcataat cagtaaaaaa
   106261 acgagcccaa ttgatagaag tccgataatg tgtcagatgg atggatttca tcagatccac
   106321 atcctgctgg tattgttcca taaaattagt tgctacggct gggccatacc cattatgcca
   106381 aacaaaatgt tcatttttat accagctatc tataaacgaa tcctgtcctt ctttttttcc
   106441 tttccaacct tccgtttgcc aagcagaaga tgcagcacca agaataaaat tttttggaat
   106501 agtaattttc atgatttcct cctgtttttt agatcccatc attttccaca cgatttgcta
   106561 cttttacaaa tggcagatag atcaaaatag aaacaataat acaaacaagt tgcgtaataa
   106621 ctgcaccgat atttcctgct gttgccagat aagcattgac gatcggcggc attggccaag
   106681 ggaccattac aactgctttc gccgcaaatc ctaaagcggt tgcaaaataa ccaatcgttc
   106741 cagtgataag aggcgttaag ataaacggaa tagctaaaat tggattcagc atgatcggca
   106801 taccaaaaat aactggttca ttgatgttga agatcccagg agcaagggat aacttggtga
   106861 tttgtttcat atcttcccgt tttccaacta acaatactgc aatcagtaaa ccgatcgtga
   106921 ctccagaccc gcccatactc atatacatat cccaaaatgg catagtaata atgttcggta
   106981 cttcttttcc agcttcaaaa gcttccgtat ttacagcaat tgccgctaaa agcaacggtt
   107041 cccgtatcgg cttcaccatt tgatttccat ggataccaat cacccaaaag acttgcgcaa
   107101 taaacatcaa tagcaaaata cccggtaacc cttgtacgac accttctaat gggcgttgta
   107161 cgacattgta aatgatatca taaaggtaca ttcctgtaag ttctttgatg ataaatcccg
   107221 cagccgcgat acccgataca gtcagaatcg tcgggaataa agcagaaaaa ctagcagaaa
   107281 cattcgttgg cacggtatca ggcattctca gctgtagttt cttctgtttc ctcaaccagc
   107341 aataaagttc aatagaaagg atggagacca acattcccaa aaataaccct ttcgtatctg
   107401 tatattgttt agccaatacg ttttctacta taacagtctg attttcattc actaattcat
   107461 aaacagttgg attaacagat aaataagaca tcactgcgat gataccaggg aatacaccat
   107521 ccactttatt cagcttggct aattcgatcc ctatcagaaa aacagcatag atggtcaaga
   107581 aacttagtgt gacatagctg atggcagatg cgataggctt taattcttct aaaaaacgca
   107641 agaaacttat ctgtgctaag ccattttccg agtcaaagac cattgctgaa aacaatgtgc
   107701 caaaagcacc cgtaataatt accggaatca atgcagcaaa tgcgtttttt atcaccatga
   107761 tataccttaa attattgatt ctcgttgcca cgttccctaa aacattggct aatttatcca
   107821 taaaactcat tccagatccc ctcattcctg cttcttctcc ttgacgtcat ctctttttgt
   107881 ctattagtca gcgcttacat ttcgatgaac agaaactatt tactcctctt ttttaccaaa
   107941 tataaaacaa aaattcatct gatgtattta tcataaaata catcagatga actgtcaatt
   108001 agtttctttt tctcctatta ttttctgtaa ttctctccga tttattgcca tatggacagt
   108061 cattgcatca aacgcagcaa gtgcattccg cctttcgatg gcctgaacga tctctgtatg
   108121 aatatcgatc gtctcgattt tcagatgctg gatatcataa tttttattaa acaaagcaat
   108181 tgattcatta atgattggga taatatgcag caaggctacg tttccgctag cttctgcaat
   108241 catcgtatga aaatcgatgt ctaattgaac atgttcttgt gtgccttctc gaaaagaagt
   108301 ttcaatcttt tgcgttaatt ttttcatttg gacgatttga ttcttcgttg catattccgc
   108361 tgccaaacct gccatcttag gctccaataa atatctcagt tcaaataagt ctcgtatcat
   108421 ccgatcctga tcttcaatca aagaaaaacc gagtgggtca ttggcaatcc ctcgcttatc
   108481 actgacaaat gtccccgctc cttgccgaac ttcaaggata ttcctggaag ctagagcccg
   108541 cacagcttct cgcaccgtgc ttcgccccac ctctaaataa tcggacaatt catattcatt
   108601 cggcagttta tctcctactt gcaactgcat ttcctttaaa tatctaagga tcttgtcagt
   108661 agttacttct actagggttt tattttcttc caaaccacat acctcctttt catctgatgt
   108721 attttttcgt aattattata acacgtttga atcatctgat gattttttgt atacaaaatt
   108781 gattaatccg ttcatagaca tacaaacgaa gaagatattt accataaaaa agactaggat
   108841 aaaattcact cctagccttc gatcattttt attttgaacg acaaaaaata tgatgttcaa
   108901 taacagacga tcttcattcg attcttgatg tcatcttttg ttttttttaa tatatggata
   108961 aaaagtttga ttacatcgtt tctattctag aatttggtcg attctcaatt cttcttctgg
   109021 tgagaaatct aaattatcca tcattcgaac actttcttgt aactgactta accgacttgc
   109081 tccgaccagt acacttgtaa cagatttttg tcgcaaattc catgcaaggg ccatttgcgc
   109141 taatgattgt cctcgtgata cggcgattgt ttgcagtgct ttgatttttt ccagagttga
   109201 tccaacttgt tcttctgaaa ggaatggtat ttccttacga tgtgctctag aatcttcagg
   109261 aataccatgt agataacgat ttgtcagcaa cccttgagca agcggactga acgtgattgc
   109321 ccctagccct tcttcttcta atactggaga tagatcgtct tctagtagtg cacgagaaaa
   109381 catattgtag cgcatctgat gaataatgaa cggtgcttcc tttcttttta aaatttcagt
   109441 catttttttt gtatcttctc cattatagtt ggatatacca atatataatg cttttccttg
   109501 tcttacaagt tgcatcaaag cttctgctgt ttcttccaaa ggtgtatcag ggtctgggcg
   109561 atgatgatag aagatgtcaa cataatctag ccccattctt tggagacttt ggtcacaact
   109621 tgaaatgatg gatttacgag agccccattc cccatatggc ccttcccaca tatgataacc
   109681 tgctttgcta gaaataatca gttcatcacg gtaggcttga aaatcttctc taaaaatacg
   109741 cccaaagttt ttctctgcag ctccagcagg gcctccataa ttattcgcaa ggtcgaaatg
   109801 ggtaattccc atgtcgaatg cgcctcttaa aatctctctt tgattatgga taggatcata
   109861 atcaccaaaa ttttgccata atccgagaga taaaatcggt aatttcaaac ctgaatgacc
   109921 ggatttacga tattccattt tttcataacg tgcactatct gcaatataca atttttctcc
   109981 tcctcatcaa atctttttaa aacgctttct tttttctatt tttagtttac tacattttaa
   110041 agaaaaggag ttaatcaaaa tgattttttc gtatattttt ttgccatttg ttttttactc
   110101 gttgatagat cagaaattga ttacgtgctt gctggatttt tttataacgt ttcggttgtt
   110161 ttttgtaaaa ttgctgatga tagttttctg ctggccaaaa cgtaccggct ggttcgattt
   110221 ttgtgacgat tggttggtgg aaagttcctg aactgtctag tttctgtttt gattgttccg
   110281 ctaattcttt ttgtcgttcg ccttgataaa aaatcactgg acgatattgc ttccctctat
   110341 cttgaaattg tccaaatgta tctgtgggat cagtaatttg ccaatagatt gtcagcaact
   110401 cgctataact tatttctctt gtgtcataaa tgatctccac agcttccaca tgacctgtgt
   110461 aaccaccact tacttgatca taatttggtt tttcaacatg tccgcccgtg tatcctgaca
   110521 aaacagatag gatccctttt ttcgattcaa acggctcgac catacaccag aaacatcccc
   110581 ctgcaaatac tgcacgttct tgatagtcag catcttgcat ggcatgcttg gaaaagtcat
   110641 atttttgctc cttatcaaat ccttccgtaa tcaataaata aaagtccatt acatcaggcg
   110701 tcagattcat tcggatagct aatggacgta gtgttgcttc tagttttgat aactgttctt
   110761 taacggatat attttctctt atgtcattct ttgtctgaat caatactttt cgttcccaat
   110821 ctcgtgtatt cggattcaaa atcagattat aaagtgtttg aatgaatatt tgttcctcat
   110881 ccatagattc tcctcctttc cgaagaagac aagctagtga ctttagctgt aatttatcat
   110941 aaagtgagga aataagcgat tacttcatcc ccctacagat ttttcgtata aaaaagagag
   111001 aatccttatc gacagattct cctttttaat caatttattt tataaatgaa aagttaattt
   111061 accttttatg gaaaactatt ttaaaaaata aacataaata tgcagttaat gtaacaagat
   111121 aagaaataat agtcgttact gtataacttg tgcttacttt aatatcagca actcctttga
   111181 ctccgcttat ttgtaccaat ccattagcat tcacctgctc ctttacaggt tgatgattta
   111241 tttctacatt cgttcctaaa taagcatata caggaagtgt gattacttgg ctcttctcat
   111301 tagttactct atatatgata gaactaccag ttgttttata aggaaggttt acccattgac
   111361 tgccaatctt tgcttcgtgt tttattatat gctccttatt ctccaatgaa gcgataggag
   111421 aataatcata taaaaaataa tccattcctt tagtggtata cgcctctact gtttctttgt
   111481 ttacaacaat atgtcccggc tgagaaatta ttttttgatt gaaatttttt ttactagaaa
   111541 cagttaaagt gattaataac aaaacagtca taaataaata aaatagttct cttgtcttat
   111601 tgtatcttct atttttgaaa aatatcatcg ttaatatcag agaagagaat attatttgca
   111661 agcttaagat ccgccaagga aattgtaaca atttggctgg cgtattttgc agaagatacc
   111721 atggcaccaa actagttgta gctagccaag tcaaacacgt accataataa atcttttttt
   111781 ctatgtttgt taatttttta aataaaatca ggggaataca taaagataac agaccaacta
   111841 gtcccattga gtaagtagtt aagtcgttgt tcatagcttc agtcagacta tcgaacacat
   111901 tagatgcatg agcatataaa tttgtcacgc taggtttgtt gattttttgg tagagcattt
   111961 gttcaaacat cggataccaa aaagcactcg tcagaaccaa tgtcattcct gccgcactaa
   112021 ataatttttt aaatatagca ataaaatctg cattcttgaa acctttagaa accaaacgaa
   112081 taaaaatgat aattaatgct gtactcatta atgttgatag tacatggctg tacactagca
   112141 aactcatact caatgctagc tttacagacg gatacttttc ttcttttcca gagatgattt
   112201 gataagcata aaataataca gggataacaa gtgttattgc gatagcttcg gcaattgcgg
   112261 aacgatagta gatgtctact gtccgataag cagagaaaat atagagacaa ctaaataaaa
   112321 atgccgcttt tttttgtttt aaaaaccgct cgccataatg ataagctacg agtgttgtac
   112381 acaagttcaa taaccaaacg taaataatat agcttacaat caaatttttt gtcatccaat
   112441 ataacattac agctggaaaa aaagtcagaa agggataaaa ataatttaca ccatatccag
   112501 tataattgaa tgtctcataa ttaatcggac tggtaaaaat agtcgataac cccttgattc
   112561 gagataagtg aaaagaaaga tctccagtgg tataaatact ctgtgtattt ttgaaaaaga
   112621 tgaaagaata tatactacta actataatta atccactcaa tattactact tttttattta
   112681 ttttcattct tcttgtttat ctccttttta gtcacatatc gagaatcttt caacttatta
   112741 ggtgcaaatc gtttaaaact tgaatacacc actaaacaat aagtcagaat actgatccat
   112801 aaagccatga gagcagttgg agtatatttt gttgtaattt ctatcttatt ttctccagca
   112861 tttacatcca cattcactaa gccatgtttt ttataagagt catttacgac tttaccgttt
   112921 acttttacct gtgtacctaa ataagcatat actggtactg tcactttttg ctttttgttg
   112981 cttgccactg tgtaagtaat gttattatct gcagctttat acttcccttt ttcccacgat
   113041 tctcctactt ttacttcatg tttttctaag tgaggccttt cttttatcgc ttctgttggg
   113101 gcgtaatcgt ataatcctcc catatctgat gttgtataac gtgggagtgt ttcttcattg
   113161 ataacaatat gaccgtgttt tgattggata acttttgcgt aattttcttt tgctgctata
   113221 gacgtgacga gtagaaacac cacagtagcg ccaatgatta accatgaata ttttttattc
   113281 atagtctttt tcataaaaac aagcgttatg atcattgaag aaaaaaagat ttggatacct
   113341 aagatacgcc aaggaaactg gagcaagctt atcggtgttt tttgaaacaa aaaccatgga
   113401 aataaattag ttacgcatac ccatgtagaa actacgccaa catagatgat tttttctttt
   113461 ttctccattt ctttcagtat gaataaagga aaaatcaacg caaccaatcc taataacccc
   113521 atagtgtatg ttgtaatatc gttatttaac gcttgaatga tactatctga aatagaaagt
   113581 gcttctagct gcaaatctgt tttgaaggga ctgtgtattt cttgataatt catctgctgc
   113641 aacataggat accaaaaata gctagttaat gcacaagtca aaaaaacagc ctttactaaa
   113701 ttcacgacca caaattttat atcgtttttt atttgcttgc ttaaaaacaa acgaactata
   113761 atcatacaag caatcaatgc actacacaat agtgtagtta agacatgagt atacacaagt
   113821 agacttatac tgacagataa agcgatgact tttttttctc tattgaatat caatccataa
   113881 gcataatata aaacagctgg gataatcgta agagcgattg cttctgcaat tgcggatcga
   113941 taatagatat caactgttcg ataagttgca aaagtatata aacaactaaa taaaaaagca
   114001 gcatctatct ttttaaaaaa cttttctcca taataaaaac
//
LOCUS       DAFEOM010000002       106752 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.002, whole genome shotgun sequence.
ACCESSION   DAFEOM010000002
VERSION     DAFEOM010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 106752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 106752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..106752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.002"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(717..3866)
                     /locus_tag="LP524_000100"
     CDS             complement(717..3866)
                     /locus_tag="LP524_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBL2233184.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYNTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3880..5124)
                     /locus_tag="LP524_000101"
     CDS             complement(3880..5124)
                     /locus_tag="LP524_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBL2233185.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELIKFKLEPGDFIMSGAGTIGRIS
                     RVPKQIKPGVFNQALIRLRINKEITDSEYFIQFIRADFMQRKLTGANPGSAITNLVPM
                     SEVKKWIVQFPILEEQKKIGNFFKQLDDTIALQQRKLDLLKETKKGFLQKMFPKNGAK
                     VPEIRFPGFTEDWEQRKFKEFSKKTGKKNTKDLDFPAYSVSNKAGLISQTEQFDGSRL
                     DDLEKTNYKFVEPNEFAYNPARVNVGSIAFNNLGMTVIVSSLYVVVKMSEDLDNEFIL
                     QFIKSPTFIKEVKRNTEGSVREYLFYENFANIKFPFTRNKEEQQKIGAFFKQLDDTIA
                     LHQRKLDLLKETKKGFLQKMFV"
     gene            complement(5117..6709)
                     /locus_tag="LP524_000102"
     CDS             complement(5117..6709)
                     /locus_tag="LP524_000102"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBL2233186.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
     gene            complement(7218..7943)
                     /locus_tag="LP524_000103"
     CDS             complement(7218..7943)
                     /locus_tag="LP524_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233187.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(8097..8423)
                     /locus_tag="LP524_000104"
     CDS             complement(8097..8423)
                     /locus_tag="LP524_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233188.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(8460..8825)
                     /locus_tag="LP524_000105"
     CDS             complement(8460..8825)
                     /locus_tag="LP524_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBL2233189.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(8862..9077)
                     /locus_tag="LP524_000106"
     CDS             complement(8862..9077)
                     /locus_tag="LP524_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2233190.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            9206..9541
                     /locus_tag="LP524_000107"
     CDS             9206..9541
                     /locus_tag="LP524_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2233191.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(9617..11614)
                     /locus_tag="LP524_000108"
     CDS             complement(9617..11614)
                     /locus_tag="LP524_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233192.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(11712..11987)
                     /locus_tag="LP524_000109"
     CDS             complement(11712..11987)
                     /locus_tag="LP524_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2233193.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            12219..12953
                     /locus_tag="LP524_000110"
     CDS             12219..12953
                     /locus_tag="LP524_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2233194.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            12992..14134
                     /locus_tag="LP524_000111"
     CDS             12992..14134
                     /locus_tag="LP524_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL2233195.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(14229..15620)
                     /gene="sufB"
                     /locus_tag="LP524_000112"
     CDS             complement(14229..15620)
                     /gene="sufB"
                     /locus_tag="LP524_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBL2233196.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(15625..16095)
                     /locus_tag="LP524_000113"
     CDS             complement(15625..16095)
                     /locus_tag="LP524_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HBL2233197.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(16082..17317)
                     /locus_tag="LP524_000114"
     CDS             complement(16082..17317)
                     /locus_tag="LP524_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBL2233198.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(17314..18600)
                     /gene="sufD"
                     /locus_tag="LP524_000115"
     CDS             complement(17314..18600)
                     /gene="sufD"
                     /locus_tag="LP524_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBL2233199.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(18613..19386)
                     /gene="sufC"
                     /locus_tag="LP524_000116"
     CDS             complement(18613..19386)
                     /gene="sufC"
                     /locus_tag="LP524_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBL2233200.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(19583..20431)
                     /locus_tag="LP524_000117"
     CDS             complement(19583..20431)
                     /locus_tag="LP524_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBL2233201.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(20455..21141)
                     /locus_tag="LP524_000118"
     CDS             complement(20455..21141)
                     /locus_tag="LP524_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2233202.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(21134..22168)
                     /locus_tag="LP524_000119"
     CDS             complement(21134..22168)
                     /locus_tag="LP524_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL2233203.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(22231..22487)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(22563..22901)
                     /locus_tag="LP524_000120"
     CDS             complement(22563..22901)
                     /locus_tag="LP524_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HBL2233204.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(22903..23259)
                     /locus_tag="LP524_000121"
     CDS             complement(22903..23259)
                     /locus_tag="LP524_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBL2233205.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(23265..24452)
                     /locus_tag="LP524_000122"
     CDS             complement(23265..24452)
                     /locus_tag="LP524_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBL2233206.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(24643..25488)
                     /locus_tag="LP524_000123"
     CDS             complement(24643..25488)
                     /locus_tag="LP524_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL2233207.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            25722..26687
                     /locus_tag="LP524_000124"
     CDS             25722..26687
                     /locus_tag="LP524_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBL2233208.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            27064..28107
                     /locus_tag="LP524_000125"
     CDS             27064..28107
                     /locus_tag="LP524_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBL2233209.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(28169..28633)
                     /locus_tag="LP524_000126"
     CDS             complement(28169..28633)
                     /locus_tag="LP524_000126"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBL2233210.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            28879..29826
                     /locus_tag="LP524_000127"
     CDS             28879..29826
                     /locus_tag="LP524_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL2233211.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(30037..32109)
                     /locus_tag="LP524_000128"
     CDS             complement(30037..32109)
                     /locus_tag="LP524_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBL2233212.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(32042..32419)
                     /locus_tag="LP524_000129"
     CDS             complement(32042..32419)
                     /locus_tag="LP524_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HBL2233213.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(32548..33126)
                     /locus_tag="LP524_000130"
     CDS             complement(32548..33126)
                     /locus_tag="LP524_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL2233214.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            33258..34550
                     /locus_tag="LP524_000131"
     CDS             33258..34550
                     /locus_tag="LP524_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBL2233215.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(34610..35728)
                     /locus_tag="LP524_000132"
     CDS             complement(34610..35728)
                     /locus_tag="LP524_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HBL2233216.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(35742..37163)
                     /gene="arcD"
                     /locus_tag="LP524_000133"
     CDS             complement(35742..37163)
                     /gene="arcD"
                     /locus_tag="LP524_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HBL2233217.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(37409..38530)
                     /gene="mnmA"
                     /locus_tag="LP524_000134"
     CDS             complement(37409..38530)
                     /gene="mnmA"
                     /locus_tag="LP524_000134"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBL2233218.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            38934..39533
                     /locus_tag="LP524_000135"
     CDS             38934..39533
                     /locus_tag="LP524_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBL2233219.1"
                     /translation="MNYFISDMHFFHEHLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(39671..40957)
                     /gene="efmA"
                     /locus_tag="LP524_000136"
     CDS             complement(39671..40957)
                     /gene="efmA"
                     /locus_tag="LP524_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HBL2233220.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(41228..41572)
                     /locus_tag="LP524_000137"
     CDS             complement(41228..41572)
                     /locus_tag="LP524_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HBL2233221.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(41709..42854)
                     /locus_tag="LP524_000138"
     CDS             complement(41709..42854)
                     /locus_tag="LP524_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBL2233222.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(43034..44008)
                     /locus_tag="LP524_000139"
     CDS             complement(43034..44008)
                     /locus_tag="LP524_000139"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBL2233223.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(44088..44963)
                     /locus_tag="LP524_000140"
     CDS             complement(44088..44963)
                     /locus_tag="LP524_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HBL2233224.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            45093..47000
                     /locus_tag="LP524_000141"
     CDS             45093..47000
                     /locus_tag="LP524_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HBL2233225.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(47541..50819)
                     /locus_tag="LP524_000142"
     CDS             complement(47541..50819)
                     /locus_tag="LP524_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HBL2233226.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(51090..51635)
                     /locus_tag="LP524_000143"
     CDS             complement(51090..51635)
                     /locus_tag="LP524_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL2233227.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(51647..52339)
                     /locus_tag="LP524_000144"
     CDS             complement(51647..52339)
                     /locus_tag="LP524_000144"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBL2233228.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(52358..52642)
                     /locus_tag="LP524_000145"
     CDS             complement(52358..52642)
                     /locus_tag="LP524_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233229.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(52655..53218)
                     /locus_tag="LP524_000146"
     CDS             complement(52655..53218)
                     /locus_tag="LP524_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBL2233230.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            53446..54108
                     /locus_tag="LP524_000147"
     CDS             53446..54108
                     /locus_tag="LP524_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBL2233231.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            54232..55428
                     /locus_tag="LP524_000148"
     CDS             54232..55428
                     /locus_tag="LP524_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBL2233232.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(55529..56905)
                     /locus_tag="LP524_000149"
     CDS             complement(55529..56905)
                     /locus_tag="LP524_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL2233233.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(56942..57808)
                     /locus_tag="LP524_000150"
     CDS             complement(56942..57808)
                     /locus_tag="LP524_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HBL2233234.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(57897..58922)
                     /locus_tag="LP524_000151"
     CDS             complement(57897..58922)
                     /locus_tag="LP524_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL2233235.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(58940..59614)
                     /locus_tag="LP524_000152"
     CDS             complement(58940..59614)
                     /locus_tag="LP524_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2233236.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(59798..60691)
                     /locus_tag="LP524_000153"
     CDS             complement(59798..60691)
                     /locus_tag="LP524_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL2233237.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(60765..62462)
                     /locus_tag="LP524_000154"
     CDS             complement(60765..62462)
                     /locus_tag="LP524_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBL2233238.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(62455..63768)
                     /locus_tag="LP524_000155"
     CDS             complement(62455..63768)
                     /locus_tag="LP524_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBL2233239.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(63768..67286)
                     /locus_tag="LP524_000156"
     CDS             complement(63768..67286)
                     /locus_tag="LP524_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBL2233240.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(67579..68037)
                     /locus_tag="LP524_000157"
     CDS             complement(67579..68037)
                     /locus_tag="LP524_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL2233241.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(68335..68853)
                     /locus_tag="LP524_000158"
     CDS             complement(68335..68853)
                     /locus_tag="LP524_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBL2233242.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(68855..69472)
                     /locus_tag="LP524_000159"
     CDS             complement(68855..69472)
                     /locus_tag="LP524_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBL2233243.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSFYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(69469..70323)
                     /locus_tag="LP524_000160"
     CDS             complement(69469..70323)
                     /locus_tag="LP524_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL2233244.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(70320..71231)
                     /locus_tag="LP524_000161"
     CDS             complement(70320..71231)
                     /locus_tag="LP524_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL2233245.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(71272..72606)
                     /locus_tag="LP524_000162"
     CDS             complement(71272..72606)
                     /locus_tag="LP524_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL2233246.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            72814..73644
                     /locus_tag="LP524_000163"
     CDS             72814..73644
                     /locus_tag="LP524_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBL2233247.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(73888..74206)
                     /locus_tag="LP524_000164"
                     /pseudo
     CDS             complement(73888..74206)
                     /locus_tag="LP524_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(74328..74522)
                     /locus_tag="LP524_000165"
     CDS             complement(74328..74522)
                     /locus_tag="LP524_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233248.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(75027..75212)
                     /locus_tag="LP524_000166"
     CDS             complement(75027..75212)
                     /locus_tag="LP524_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233249.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(76062..76403)
                     /locus_tag="LP524_000167"
     CDS             complement(76062..76403)
                     /locus_tag="LP524_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBL2233250.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(76403..76633)
                     /locus_tag="LP524_000168"
     CDS             complement(76403..76633)
                     /locus_tag="LP524_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL2233251.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            76920..77258
                     /locus_tag="LP524_000169"
     CDS             76920..77258
                     /locus_tag="LP524_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233252.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(77463..77702)
                     /locus_tag="LP524_000170"
     CDS             complement(77463..77702)
                     /locus_tag="LP524_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBL2233253.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(77678..78025)
                     /locus_tag="LP524_000171"
     CDS             complement(77678..78025)
                     /locus_tag="LP524_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBL2233254.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(78757..79338)
                     /locus_tag="LP524_000172"
     CDS             complement(78757..79338)
                     /locus_tag="LP524_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL2233255.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLVKERLLTSQKVL"
     gene            complement(79521..80147)
                     /locus_tag="LP524_000173"
     CDS             complement(79521..80147)
                     /locus_tag="LP524_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL2233256.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(80169..81500)
                     /locus_tag="LP524_000174"
     CDS             complement(80169..81500)
                     /locus_tag="LP524_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HBL2233257.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(81655..82056)
                     /locus_tag="LP524_000175"
     CDS             complement(81655..82056)
                     /locus_tag="LP524_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBL2233258.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVFEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(82142..82348)
                     /locus_tag="LP524_000176"
     CDS             complement(82142..82348)
                     /locus_tag="LP524_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233259.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(82443..83945)
                     /locus_tag="LP524_000177"
     CDS             complement(82443..83945)
                     /locus_tag="LP524_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBL2233260.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(83963..84418)
                     /gene="argR"
                     /locus_tag="LP524_000178"
     CDS             complement(83963..84418)
                     /gene="argR"
                     /locus_tag="LP524_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBL2233261.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(84516..85133)
                     /locus_tag="LP524_000179"
     CDS             complement(84516..85133)
                     /locus_tag="LP524_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBL2233262.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            85354..86727
                     /locus_tag="LP524_000180"
     CDS             85354..86727
                     /locus_tag="LP524_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL2233263.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            86802..87914
                     /locus_tag="LP524_000181"
     CDS             86802..87914
                     /locus_tag="LP524_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBL2233264.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(87986..89623)
                     /locus_tag="LP524_000182"
     CDS             complement(87986..89623)
                     /locus_tag="LP524_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBL2233265.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(89791..90399)
                     /locus_tag="LP524_000183"
     CDS             complement(89791..90399)
                     /locus_tag="LP524_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL2233266.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(90784..91935)
                     /gene="queG"
                     /locus_tag="LP524_000184"
     CDS             complement(90784..91935)
                     /gene="queG"
                     /locus_tag="LP524_000184"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBL2233267.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(91954..92706)
                     /locus_tag="LP524_000185"
     CDS             complement(91954..92706)
                     /locus_tag="LP524_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233268.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKNADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(92847..93797)
                     /locus_tag="LP524_000186"
     CDS             complement(92847..93797)
                     /locus_tag="LP524_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBL2233269.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(93798..94637)
                     /locus_tag="LP524_000187"
     CDS             complement(93798..94637)
                     /locus_tag="LP524_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBL2233270.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(94634..95380)
                     /locus_tag="LP524_000188"
     CDS             complement(94634..95380)
                     /locus_tag="LP524_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBL2233271.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            95618..96988
                     /locus_tag="LP524_000189"
     CDS             95618..96988
                     /locus_tag="LP524_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL2233272.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(97043..97939)
                     /locus_tag="LP524_000190"
     CDS             complement(97043..97939)
                     /locus_tag="LP524_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL2233273.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(98053..98223)
                     /locus_tag="LP524_000191"
     CDS             complement(98053..98223)
                     /locus_tag="LP524_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233274.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(98220..98897)
                     /locus_tag="LP524_000192"
     CDS             complement(98220..98897)
                     /locus_tag="LP524_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBL2233275.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(98988..99893)
                     /locus_tag="LP524_000193"
     CDS             complement(98988..99893)
                     /locus_tag="LP524_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBL2233276.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     KNFKNIALDTLKKQLEKQTTVSWTT"
     gene            100068..101522
                     /locus_tag="LP524_000194"
     CDS             100068..101522
                     /locus_tag="LP524_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL2233277.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            101538..102569
                     /locus_tag="LP524_000195"
     CDS             101538..102569
                     /locus_tag="LP524_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBL2233278.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            102576..103763
                     /locus_tag="LP524_000196"
     CDS             102576..103763
                     /locus_tag="LP524_000196"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBL2233279.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            103919..105301
                     /locus_tag="LP524_000197"
     CDS             103919..105301
                     /locus_tag="LP524_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBL2233280.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            105273..105659
                     /locus_tag="LP524_000198"
     CDS             105273..105659
                     /locus_tag="LP524_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233281.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(105859..106527)
                     /locus_tag="LP524_000199"
     CDS             complement(105859..106527)
                     /locus_tag="LP524_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBL2233282.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT        30578 a        22269 c        18801 g        35104 t
ORIGIN      
        1 gactaataca ggtagtcctc ttttttgtta ttttgatatt ctaaatcatt ttttattgga
       61 ttattccctt gttcgatata gacccaaatt gatctatcaa taattatcaa actgagtcat
      121 atagagacaa aaatttcaaa aatgacctgt taaatggtca tcgaatatca tttaaaatca
      181 atacgacgga gtcagacacg gagtcaaaac aagcccgctg agagtaaaaa atatatacag
      241 tcccataggt aaagtgccac aggactgtat atttttttat ttgagattac ctgaaatgat
      301 tccatttact ttttatctca ttttttccta ctagttagta tcaaatactc tcctattttc
      361 tcaataaact attttgggtt acatggtatc tgaaacagct aaaaccttgt cattacagca
      421 aaaaatcact ttatctccgt ttcatttcag atacatttca cttaagcgga ttccaaaaaa
      481 acaaaagaaa aaggaacccc tagttagcct atgcttttta ttggttcctc tcaaaacacg
      541 gtcatataac aacggggtgg ggtaaatgaa tgaaaaaggt ggaggaaaca ttattcaata
      601 gctgtcttaa atacttcgtt ataaaatgtg aacaaataaa agacatctat ccctattttc
      661 tacacttcca ctttactacc atctttgaat cgtacaattt tcttaactct tctcgtttac
      721 ctcaattcat tttcaagtgg aatcagctca tcatctaaca ttttacgcca tgctcgcttc
      781 atttcttgtg ggtatttaaa tggcttcgct tctggatggt tggctttata tccttcgtaa
      841 tccttactgt tgataattgc tttcatatta ggaattggat cttcacctgg catatattgc
      901 agattagaag catgtaattc tttctcagaa acaaaccact cattggaaaa gacatgaatc
      961 aaatcatcac tcgcttgtgt aaaatagcga cgtttaatga caaagatatc ttcattacta
     1021 tctaaagtcc ccacatcaaa cgcatttaaa atttgtcggt aaacttcttt gactccttct
     1081 gttttattca atttagccaa agctttttca atttcttctc gtgcatctga actattcgcc
     1141 gcataagttc ctagtaattg atcaatataa gtagaatcaa tatcatacaa cttcgtggta
     1201 ttttcactat aaaattcgat cgtggaaaaa tcttctccct ctttttctgg ttcttcctct
     1261 attaatgaac ccttcacggt ctcgtacacg ccaatttgtt cttccaaaag ttgaatttga
     1321 ttgttcaagg ttgaagattt caccatgtca tcattgtaat cgtcataagt cactaacgat
     1381 tcataagcac gattcaattc ttggaatgct ttgacgtact cgatacggat ttcaagtggc
     1441 gtatgttcat caatggattc catggatgaa acctctttta acttaccata tgcttgcttg
     1501 aatctttttt tggattcctg ataacttgga taaatcaaac ccgattcttc ttttgcttct
     1561 gaatacagtt ttgtcgcatc ctcgacattc ttctccattg tggctggctt tcggaaagta
     1621 acaattagtc cttttgtttt ttctggatac gtacggttcg tacgtgaaaa agcttggatc
     1681 aatcctgcat actctagatt acgatccaca aatagggttt gaatcgtagg tgcgtcaaaa
     1741 cctgtcaata gacgatccac tacgatcact aaatcaattt gtttcccaaa ttgtttgaat
     1801 tccgctcttt ttcgtgccaa acgattattg atgtctccgt tataacgctc aatgtctgct
     1861 aatgaccagg aagtattata atactgatta tattcctcaa taattttttg catttcttct
     1921 tgttggacac ttgaatctcg gctgttttct tctaatgaat aggtaatagc gattcgtgga
     1981 aaatcaggat cctccatcgt tcttccttca cgaatgggct gattaggaaa ctcttttgtt
     2041 aaccagtcag gttctttcgt catttctttg atcgctcgat aatacttttt ggccatgtca
     2101 atggaactcg tggttaagat cgcagacttc gttggacggc catttcgaaa atcaaatttt
     2161 gtataggcat tgtctggacg gaacatttta tgaatgactt tttggatatg ttcatctgct
     2221 tcaaaaacag tagggtccaa ataactttct tttttgacag gctccatttg atcaatcata
     2281 cgattgatct gttccggtga atactctgca tatgtttcta cttcttttaa tttccgatag
     2341 attttattct ctaatgaagt cggctccacg gtgttttcat gctctacttg aaatcctaaa
     2401 accgagccat cttctaatgc attcttgatg gtataggtat gcaagacctc tccatactga
     2461 tcatgggtcg tacgtgccaa ttgccctttg gcttgtttcc gattctcagg gaaaatcggt
     2521 gtacctgtaa agccaaacca agtggatttc ggaaacattt ttttgatttc ttccatattt
     2581 tcagcactta acgctcgatg acactcatct actacaaaca cgatatgttg ccccattaat
     2641 ttttggaagc gattggttcc ttttttctct tcttgttttt ttgcatattt caacgccgca
     2701 tctagctttt gacgggttgt aataatcaca acatttgcat tggcatctgc caacagagtt
     2761 tcacttaact ctttggcact tcctgttccc acgatcaagc tattcgcttt ggcgttcccg
     2821 gaagaaatcc cagtattaaa ttccgaagca aatttcgtaa actctgtggt ggtttgatta
     2881 tctaaatctt tgcggtccac taacatgatc gtccgatcga tccccgattt tcgtgctaaa
     2941 agcttagtag atacaaaact agtcaacgtt tttcctgaac cagtcgcatg ccacacatat
     3001 cctgattgat gtttatttgc agcaataaac aaggcttgaa tcgcatgtac ttgataagga
     3061 tgtaacacca tgaggatttt attgtcttga tcttcactga caatcgtata atccgcaatc
     3121 aagcgatgag catccgggat atttagcact tgtttgcaaa actcatataa gttttctacc
     3181 tttttattat ctttcgttcg ccaactaaat aaaaacttag gatgcaaatc ctttggtaag
     3241 gcatttgcaa aataccgagt ggtttgttca ttggaaacca caaacaactg taaggtcgaa
     3301 aagatattgt tccgaaacat accttcttct gcatattttt tgatttggtt aaacgcctga
     3361 taaaaactat cttttgcggt tacttgtttc agctcgattt ggacaatcgg taatccattg
     3421 atcagtaagg tgacgtcaaa acgtcgatcc cgtgcttcta tgttacttcc acgttttgca
     3481 atttgatgga ccacttcata ggtagagatt cctccaccaa tgtcttgatt agaataaaga
     3541 atgagcgaga cagaacccag ttgcggatct tcccgttcaa tcgtgatccg agccattcca
     3601 ttttcacctt ttaaccattt cgcagcatca aagggggttt tcgttcgtag taacaattct
     3661 gttttgatcg tttcaaattc tttatcggtc aaaggaaaat cattcagttc tgcttgatta
     3721 tttgagatga ttttttggcg taaattgacc caaagatctt cttctgattt tagatcggga
     3781 cgatacgtcc actgattatg cccttccccc aatacacgga tcaactgttc ttcgacttcc
     3841 gcttcctcac ggtgactaat tttcatttat cttccctccc taaacaaaca tcttttgtaa
     3901 gaagcctttt ttcgtttcct tcagcaaatc taacttacgt tgatgaagag cgatcgtgtc
     3961 gtcgagttgt ttgaagaaag cgccgatttt ttgttgttcc tctttattcc tagtaaaagg
     4021 aaacttgata ttagcgaaat tctcatagaa taagtattct cgtacgcttc cttcagtgtt
     4081 gcgcttgacc tctttgataa atgttggtga cttgataaac tgaagtatga actcattatc
     4141 taagtcctcg ctcattttca caactacata aagagagcta acaatgaccg tcataccaag
     4201 attgttaaat gcaattgatc ctacgtttac tcttgctgga ttataggcga attcatttgg
     4261 ttcaacgaat ttataatttg ttttttctaa atcatccaac ctactaccat caaactgttc
     4321 tgtttgagaa attaaacctg ctttattact taccgaatag gcagggaagt ctaaatcttt
     4381 cgtgttcttt tttccagtct tcttactaaa ctctttgaac ttacgctgtt cccaatcttc
     4441 cgtaaatcca ggaaaccgaa tttctggcac ttttgctccg tttttaggga acattttttg
     4501 taagaatcct tttttcgttt cctttaataa atctagctta cgttgttgaa gagcgatagt
     4561 gtcgtccagt tgtttgaaga agttcccaat tttcttttgt tcttctaata ttggaaattg
     4621 tactatccat tttttcactt ctgacattgg tacgagattt gtaattgctg aacctggatt
     4681 agctcctgtg agttttcttt gcataaaatc tgcacgaata aattgaataa aatattctga
     4741 atctgtgatt tctttattta ttctaagtct aatcaaagcc tgattgaata cacccggttt
     4801 tatttgcttt ggtactcttg aaattctccc aattgttcca gcgccactca tgataaaatc
     4861 gcctggttct aatttaaatt tgattaattc attatatttt ttttcagtaa ttttatatct
     4921 tgtttcgtaa ttatcataaa tagcattttg ttgttcatag acaacaaaat tgctattaga
     4981 tacaaagaat tctttcttta aggcactccc aaatggtccc ctccttactc catctttttt
     5041 atcaagtaag cattcaaact tacgctgttc ccaagcttcc gtaaatcctg ggaaacgaat
     5101 ttctggttgt atatcattac tcatgtttga acacctccag cgcaccttgt aaccacgctt
     5161 cattttcagg tgttgtttgc agggaagaga tagcttctaa aagttctttt tctagcactt
     5221 gctgttcttg tcgtatcttc ttgatgtcat ttccgacatg aaccatatca attggttctt
     5281 cttcttcaaa cgtatctaca tagcgtggaa tatttaagtt atagtcattt tctttgattt
     5341 catcaaagtt tgccaaatga gcgtatttct cgacatcttt tctttcagca taggtttcga
     5401 ggattttttg aatgttttct tccgataatt tattctgatt ttttcctttg acaaattctc
     5461 gactagcgtc aatgaataat acgtctcgag tttggcggtt ctttttcaat acaatcaccg
     5521 tcgttggaat cgatgtcccg aagaacaagt tcgcaggcat accgatcaca gcataaatac
     5581 tgccgtcttc tagtaatttt tgacgaatga ccccttctgc agcgccacgg aataaaactc
     5641 cgtgtggcaa gacgatggcc atagtccctg tttccttcaa atgatagaaa ccatgaagaa
     5701 gaaacgcaaa atctgctttg gattttggcg ctaattttcc ataacgatta aaccgagaat
     5761 cgtctaagaa ggtcgtatct gctgaccaat tagccgaata aggtgggttc attactactg
     5821 catcaaatgt gtaaggttca tctgttggcc aatccttatt caacgtatcc ccattgcgga
     5881 gattcatttc ttcggcatct accccatgta aaatcaagtt catttttgct agattgtatg
     5941 tcgttgtatt taattcttgc ccatgatatt taacgttatc tgggtgagtt aagtagttac
     6001 gcacatttaa catcaatgaa cctgaaccca tggttggatc aaaaacacta aagaatcgtc
     6061 gttctttttg atccaacgtc acgatttgtg ccatcatatc agaaaccata tgaggcgtgt
     6121 aaaattctcc ggctttcttc cctgcttctg aagcaaattg actgatcaga aattcataag
     6181 catccccgat cacgtcccca tcgtgaccta aaacttccac gtcattcaat ttcttaatga
     6241 cttcggtaat cgtcacattt cgttgttgct catctgttcc tagttttttt gattggagat
     6301 ccacatcatc aaataaccca ttaaattgat tgtaggtaga tgccaattgc acaaaggctt
     6361 tgtttagatc attcaattgg aacgttgctt gttttgcttg atccgctaag acgttgaaaa
     6421 gatactctgg cgaaatcgca taccccaaaa tatcaacgat tgttgcaatc agatcttctt
     6481 ttgattcttc atctgaaagt aattcgcgat acaacatcgt ttgtttgctt accgtatcat
     6541 attcctctaa ggattcatct gctaaaagaa ccacttgttc caacaaacgg tcggataaat
     6601 atttatagaa aatcagtccc aataaataat ttttatattc tgacgcatcc attttgctac
     6661 ggagattatc tgccgcacta aataattttt gatttaattc tgcacccaaa aagatacact
     6721 cctatctctt atttaaagtt gctatatacc ctcttatttt agcatactcc cttttagaaa
     6781 aagcaaaaaa aaaagaaaga aacttattct tgctaataaa ctacgcgaaa ggagtctaac
     6841 gttgtttgat ataacttaga ctgatcgtta acttattttt tatatttcta catagttatc
     6901 tttagaacca tttcagatac atatgaaaca attacatatg attttttcat aaaaaaacag
     6961 tactgtctag tagtataaaa tatacaaatt tcttaaaaat tttttcacta aaatcactac
     7021 ctgaaacagt ctaaaacact tatatatcaa cgtttaatga aactatctcc gtttcatttc
     7081 agatacaaat aaatatagtc gggctccaaa tccgaaaaaa aataccccca gtagcttatc
     7141 ctaagctact ggagatgtat aagtacggtc atataacaac ggggtagggt aaataaatga
     7201 aaaagatgga agaatcatta ttcaataact tttttaaata cttcgttgta atcagttacc
     7261 tgtccgttct taaataccac agaataatct tttttcccat tttcaagtaa ccgaagtgga
     7321 atatctttac caactttctc tgtcactgca ttactgatac ctgttagatt agctaataaa
     7381 tctgataaag attgagcatc tgtagaaatc tctgtatcag tatttatcgt gattccttta
     7441 tcttcattta caagtttcca agtaggtggt atagctgtgt atgtttctac aaatgaatta
     7501 agtttttcat taagtgactt ctcttttata tttccatcta aaaactctgt atatatggct
     7561 tgagctatac taaaacaaag ctggccttgt tctaataatt ttacatcatt agttagtaaa
     7621 gctgtgttca cgttatcaat cgcttcaaga agagtatctt tggtgtccct aacagcttta
     7681 ggtgtgcctt ctggaatttc cctacttgat acctcgtcca ttttatcttc cataaacccc
     7741 ttaaaatcat atctttctcc caaatccata gaagtttttg agaaagcttt catttctgag
     7801 tctatttctt caataatatt taacatgtct gatacatatt gtttaggctg attcgttttt
     7861 aatctatatg tggtaaatat ctcaattcct ccaattatca ataaaattgc caaacaaatc
     7921 cataataatt ttttcttttt catttttcct cctatacact gctgttataa cagtgactaa
     7981 tgtaaagatt ttcccagcaa tcatcagact attatttaca gggaatcaaa aaaactcctt
     8041 agtaagctta cacctttagg agttcctgtt tgaacggtat gcataataca tttaaatcaa
     8101 tttaacatta acacctcagt atatggtatc tctacatata cacggtcttt ttttggatag
     8161 tactcgacat ttatattgat agcacttcca ttgtctaacg tagctttagc ccggtacgtg
     8221 ttatcaccat tatcatgagt gatcgtcaca tcatcacatg tccgatttaa gttatactgt
     8281 tcttgtaaaa tctgagttac caatggtaca gatgacttct ctagtccaga tgaacccgtc
     8341 attcttgaaa aaattatacc tataatagcc aatacaacga tacctataat gacaaacttc
     8401 ttattcatat ttactttatt caacttttta tctcctatct ccaaaatgat tttctctcat
     8461 cagtaactca ttgacatccc caatagtaca ccaaagatgg ctccagctac ccctgctatc
     8521 atcaaaatcg tacccatttg ttcatctttc tctcgatata agtaacccat aataacagca
     8581 acagcaccaa ataccacagg aataaatgct aatgatacta ccatagaaat ccaaccaata
     8641 atcaaataca gcttccctgt ctcttgtcca tgtacgattt gttccttcat ttcattaatt
     8701 gtctgctcag tacttttctt tactgtgtct gttccggttg tctcatctgt ttcttgtaat
     8761 gctccgcaaa actggcaata cttactttca tttgttattt ctttaccaca ttttctacaa
     8821 tacatgtctt ctcctcctaa aatcattcaa ttgcttatta gctaactgtt tactttatta
     8881 aatacatcag aaaacagctc atttattggt acacttaata tctcagaaag cattttcatt
     8941 tgatagggtt taggaatcgt tctccctgtt tgccacgaac ttacacattg ctgtgtaaca
     9001 ttcatttttt tggctaactc agtttgggaa agtccttttt cttttctgta tcggctgaga
     9061 ttacttactt ttttcatgat tctcccctcc tgttttatta tatacaacaa tatgatgtat
     9121 ttcaactcat aaaatatata aatacttcaa atgtcattac aaatacaaca acttgttgta
     9181 ctctactgag aaggggggtg tattaatgaa acagaagcaa tatgcaaagg tcattagaga
     9241 aaagcgaaaa gcactcgggt ggacacaaaa agaattagct aaaaaaatct tctctacaca
     9301 acaggcagta gcaagatggg aaaattctgt aacagaacct aacctcgata gcttaacagc
     9361 tctttctaga gccctaggaa cccctgtaag ccattttcta gacaatgttg acatagatta
     9421 tgaggaagag tttttggctc tctatcgctc tcttagtact gaagatgcaa ttcgaacaat
     9481 tgactatatg aagctattaa aaaggcaaga aaatgaacgc aatcagctat tgaaagattg
     9541 aaaaataaat aacccaccga actttaacgg tgggtgtatt gagatgaaat aagattaaga
     9601 gctatggata acctgtctag tttaatataa ttgggtctct tctattcctt actctccgtc
     9661 catcatctct tgttacactt gtaaattgat aaataggaac taattcaact cctgataata
     9721 tcaattcttt tattacttca tctatccctg cccctttcgc tagatatact aaaaaactac
     9781 tgcttgcttt cacattgtat agtttcttaa tatccttaga agctcgttca agagtgaatc
     9841 cttgtttaac aaagtagcct ctgttttcta ttaaagcatt tttcacgctg ttatccctga
     9901 catttcgtag gcgttcacaa acagtgttaa taagttcttt ttctgtcatt agatatgtcc
     9961 cttgccacga taagccctgt aaaacttcat gtgcttcatc taaaatcaat tgtttttcct
    10021 tgctaagttc tgatcgggaa ttttgaggaa atactctttt agcctctttt tcgttaaaac
    10081 atatcttaac ccattcataa gaaaaatatt gatatgtaaa atgaaatttt tccagaagtg
    10141 gaaggatatc ttcattcaat ttttgaatca cagaagttgc attaaaaacg tctaatcgat
    10201 agacattgct tctcttatgt ttaaagtttc ctaacgcttt ctcttccttt aacctattta
    10261 ggtattcgag catcttcaaa ggaacatctt catcatctaa tttttttatc aatcctataa
    10321 aagtcataag tgatagtaac ttattacatt tatctatgtc ataagtttta ccatagtaag
    10381 aactcatttt tttagctaat tgttcccccg acaaaataca agtcacactt tccttactat
    10441 catcaaaaaa attaacatca gaaagcaaga catcatagac acgtcgcatt tcctctagat
    10501 aattccttat tgtcctgaaa agtttgtctt tcattgattc aagagtgtca aaaagtactt
    10561 ttgattcctc tttcaatttt cgaaaatgtg taggatacaa gtctaggttc tctaatagcc
    10621 aattaaatgt ttctacccga ctgtctttct ttaaaattag catcacaact ccaataaaat
    10681 tttcactgat atcacaatta ctggaatgac aataaaaaat ggaatgccca gttttactgg
    10741 taaaaatact agcagaggga ttatgatcgt tgtgaaacag acatcttaag tttttacttg
    10801 acttaagtaa tttattcata tcaatggtta acaatctttc ataaatctca ttaaaagaca
    10861 tgtcggagga atcaaacagg cagtctttaa accactgttt catttcttct ttgtcgtcat
    10921 tttttatcaa cttaacaaaa tcggtttcat tgactagttc atactctctc cctgaatcac
    10981 tttgacaatt tttagaacat gtcatggtaa aatcaatcgg aagctcagta atatctaaca
    11041 tgttgtcttc attaattatt atcactgcat ctgacttata tcctccaaaa aacattctat
    11101 tcgaagcatt tacattgtca tcacacccgg ggatgttctt tttcaagtat tcgtaaacag
    11161 caaccatttt accatagtca gtaattgctt cttttaatag aaagacaatc ttgtattttt
    11221 ctagattttc agagtaccga atactttttt gtatcgcaaa cgcagaatta tgaactaaat
    11281 tgatagataa aaccttttct atgcttaagt atctactatc atctttactc aaatctacta
    11341 ccttcccctt ctcatcttta acagtgttat cgacatccag aataattact ctggtttcca
    11401 caaaactctc cttgtcattg cctttattct ttgtcttgct tggaataaat gattgtgatg
    11461 aatttagtat ctctacaaac tcatctcttg taacttctct atagttatct aaacacctat
    11521 ctcttacatc agttttttct ttttttgtaa ctggcttctc taataatttt tcttgatcta
    11581 cataaattgg aatattaatc attgatgttt ccatttgtgt ttcctcctta taaaattctg
    11641 ttaaagacta aaacatacag ataataaaaa caaactcttt attactatat gttttagtct
    11701 tatttgttta ctcaaccttt ataccgttcc atgaaactat caatatcaga ttttttaaat
    11761 ctaatgaccg tatcaatttt tataacaggt aacccgtatt gagaaacata ttttctaatc
    11821 gttcctactg atacacctag atattcactt gcctgctgat agtccatata ctctagagtg
    11881 actccagaaa taatgtcttc tagcataggc tttagcactg taattatctc tttcacttgt
    11941 tgttcaactt gtatagaaac aatgctatca atgatttgta gagacatatt tccccctcct
    12001 ttcgtgattg cttgaacaaa ttttctaaga attatcttcg tattttttgc tcaaaataaa
    12061 agtattttgt tattaaccga ttaatcaaac aaaaaatgat taccagttca tcgacataca
    12121 cataatacct cgataaaaaa ggggtgtcaa cagctttgta aaatttattt acaacacccc
    12181 ttttttgagg tatactaatt ttaggaggtg tggtcaatat gataaaacgt ccaccaataa
    12241 attatttaga acgaaaaaag atactcggaa caaaaataaa agctataaga aagagtaaaa
    12301 aattaacaca accagcattt ggtcttatga ttaacaacgg tcaattaatt gacaaaaaaa
    12361 ctatctacga atgggagaag ggaacctatt taccaatacc agagcgatta tcccgaattg
    12421 ctgatttggg aaatatgagt atagaggagt tagtttgtgg caatgtagaa gaatatattt
    12481 tgggaattat tctttataga gatagcattg tactagatgg tataacattt ccagataaaa
    12541 acctgttcca gcatcttcgt caacagttcc caccagtaca ctctaattta gatacatggc
    12601 tggatagata ttccaaatta gaaccagaga tgcaagaatt tattgctaac aagacttgta
    12661 ataaagttaa aaatgaaaaa attagtcttt tcaatatctt aaaaatagaa gaattattca
    12721 tcaatgctat tgtagaagaa tttgacaata acattctttt cctcactagc agtatagaag
    12781 aactactaga aagaatggta gatgaatggc tacctattca attgaaagac atgtcttatc
    12841 ccgaagaagc tgtgagagaa attactgata atataaacaa gctagaacaa acgatttcat
    12901 caattgggaa aaaatacact aagaaaaaaa tgaagggagg tgataccatc tagtggtaag
    12961 tactccttgt aaactaagag gagatgattt aatggcaaaa atatcaaagt acaaaaagaa
    13021 aaacggtcaa gtggcgtgga tgtataaagg acacattgcc accgatccac gaactggtga
    13081 aaaaataaat actacgagaa gaggttttag tagcaaacaa gaagctcaaa aagattatga
    13141 cgagtacaga catcaaatat tatacggtgt taaaaagaaa gcacctgata tgacatttga
    13201 agacctgtat aatgaatgga taactcatca gcgtaccagt gttaaagctt caacaatcgc
    13261 aatctcagta agatacgcaa ataatcaaat acttccagca tttggtaagc ttaaactatc
    13321 gaatatctct gtcccatatt gtcaaaaagt ggtagatgaa tggcacagta aatatgaaag
    13381 ttacgattat atgagaaaac agactgcaca aattttacgc tacggggttg caatgcaata
    13441 tattgacaat aatcctatgg aaaaaacact tcttccacga aaaaaagagt atgagaaaaa
    13501 tcgtaaattt tactcaaaag aggagcttaa taatttactg gatgctttta aagattttgg
    13561 taacatgaaa caatacgcct tcttcagatt attggcatac acaggtatga gaaagagtga
    13621 agtactcgct ttacagtgga aagacataga cacgtttaat aaagaactac atgtcaataa
    13681 aactcttgct gtagatgaat ttggtaaagt tataattcaa tctccgaaaa caagagcttc
    13741 tagacgagtt atctctttag atactgagac tctttctatc ctcaacaact ggaaattaca
    13801 gcaaaaagaa gagtatttaa aacttggata caatactagc tctaaagagc aacatgtttt
    13861 tacaacagtt aaaaacactc tatacattcc aaacactgtt aatgattggt taagatatat
    13921 tttgaaaaaa tataacttgc ctcgtattac acctcatggg tttagacata cacatgctag
    13981 tttattatta gaagcaggag aatctgtcaa ggtagttcaa caacgattag ggcatgaaaa
    14041 tagtaaagtg acattagaca tctatgcaca tattacaaat aatgcaccta agaaaacagg
    14101 tcaagacttt gctgatatga tggctcatca gtaaaagagc tggtcaaaat ctggtcattt
    14161 ctagtataca ggcaaaacaa aaaagctaga acccttatat atcaagggtt ctagctccta
    14221 taacaggttt acccaacact gccttccatc tcataactga tcaatcgatt gagttcgact
    14281 gcatattcca ttggaagttc tttggtgaat ggttcaacaa agcccatgac gatcatttca
    14341 gttgcttcag attcggtcag gcctcggctc atcagatagt agagttgttc ttctgagatt
    14401 tttgagactt tggcttcgtg ttctaaagag acttggctgt tgtggatctc attgaatggg
    14461 atcgtatcgg atttcgacca ttcgtccatg atgatcgtgt cacattcgat atgagagatc
    14521 gaacccgcac tgtttttggc aaaggttact tgtcctcgat agtttacttc tccgccgtct
    14581 tttgaaattg atttagagac gatagagcta gatgtatttg gtgcattgtg gatcattttt
    14641 gcacccgtat cttggatttg gtttgctcct gcaaaggcga tggagagcat ggtgcctcgt
    14701 gcaccttttc catctaagta aacacttggg tatttcatcg ttgtttttgc acctaggttt
    14761 ccgtcgatcc attcaacggt tgcgccttcg tatgcttttg ctcgtttggt tactaagttg
    14821 tacacattgt ctgaccagtt ttggatcgtt gtgtaacggc aatacgcatc ttttcttgta
    14881 aagatttcta cgatggcggc atgcaggctg ttacttgaat aagtaggtgc cgtacaacct
    14941 tcgacatagt ggatgcttgc tccttcgtca acaatgatca gcgtacgttc gaattgcccc
    15001 gtgttttccg cattgatccg gaagtacgtt tgtaatggaa catcgacttt gacgcctttt
    15061 ggcacgtaaa taaaggtccc acctgaccat acggctgaat tcagcgctgc taatttgttg
    15121 tcggttggcg gtacaagttt tgaaaagtac tctttgaata attcagggta ttctttcaaa
    15181 gcagagtcag tgtcagtaaa gacgattcct aacttttcga attcatcttt catattatgg
    15241 tacacgactt ctgattcata ttgtgcggaa gcaccggcta gataagctcg ttcagcttct
    15301 ggtataccaa tcttttcaaa tgtatctttg attttttctg gtacgtcttc ccagtcacga
    15361 gcgggtttgt cacttggttt ttggtaatat ttgatggcat cgaaatcgat gtctgaaagg
    15421 tcggggcccc atgtttgcat tggcattttg ttaaatgttt ctagagattt cagacggaat
    15481 tcaagcatcc attctggttc gcctttgatt cttgacatct cacggatcac ttcttctgtc
    15541 aagccttctc ctgtactgaa aaccggttcg acatcatcgt gaaacccgaa tttatattct
    15601 tctaattctg gtacgcccat gttctcactc ctcttccttg tggtaatgat ggaaagctgc
    15661 ggtaccatcg ttttccattg cttttcctag tgctttccag gcaagtgtcg cacatttgat
    15721 ccgagcggga aatttagcta caccaccaag catagcagca tcgcctaatt ggtcgggatc
    15781 tttgacttct tttccttgga caagctgtag aaagtctaca gttaaatgtt ctgcttcttc
    15841 tattgatttt ccgatgacgg cgtctgtcat catcgaggca cttgccgtac tgatggagca
    15901 gccactgccg tcaaaggcga tatttttgat gattccgtct tttacatcga cttgcaattc
    15961 gatcacatct ccacatgtgg ggttgttcaa ttcgatcttg cttgtcgagt cagtcaagct
    16021 tccacgatga tgcggatggg aagaatgatc caagatcact tgacggtata aattgtctaa
    16081 tttagatagt cccatgttgg aaaaactcct ttgttgccat gattgcttca actaaacggt
    16141 cggcgtcttg cgttgtgttg tagaaataaa agctcgcgcg agcagtcgct gatacttcaa
    16201 ggtacttcaa tagcggttgc gcacaatgat gacctgctct gacggctacc ccttccatgt
    16261 ctaaagctgt cgcgacatca tggggatgta acccatccaa attgaatgca acgactcctg
    16321 tccgaaaatt cgggtcttgt ggaccataaa cagtcaatcc atcaattgct tgtaattttg
    16381 gcaacacata agcggctagt tcttcttcat atcgatggat attgtccata ccgagttttt
    16441 gcaaataatc gattgcatgg cctaatgcga tagcgccggc gatatttggt gtccctgctt
    16501 cgaatttcca aggtagttct ttccatgtac tgtcgtataa ttcgacaaaa tcgatcattt
    16561 cgccaccaaa ttcaacaggt tccatttctt ctaaccattt tcgtttacca tataaaacac
    16621 caatacctgt tggtccgcac attttgtgtc cgctgaaagc aaagaaatca cagtctagtg
    16681 cttggacatc aatggccatg tgcggggcag attgagcacc atctactacg atgaccccgc
    16741 cgttttcatg gacaagttca gctaattctt gaacaggatt gaagacacct aagacattgg
    16801 atacatgagc aatggagacg attttggttt tgtccgtaat ctttttcgct gcatcctcca
    16861 tatccaggaa gccttcttct gttagttcga tatatttcaa gacagcacct gttcgttttg
    16921 ccaactgctg ccacgggata acattagaat gatgttccat gtaagagagc actatctcgt
    16981 ctcctgcttt gatatagcgg tcgccgaagg ttctggctac ccaattcaag cttgtcgtcg
    17041 taccacgagt aaacagcact tctgctgttt cttttgcatg gatgaacgcc cgtacttttt
    17101 ctcttgcttg ttcataatca tgggtcgcgc gttctgctaa cgtatggaca ccgcgatgaa
    17161 cattggcgtt atcgtgttcg taatagtacg ctaatgcctg gagtacttgt tttggctttt
    17221 gggtcgtcgc tgcattatcc agataaacta aaggttcatc attgacgtct tgaaataaga
    17281 taggaaaatc attgcgtagc tttttagcat cgatcatcca ttcaacttcc cttcgattac
    17341 ttcgactaat tctttccgaa cttgttctac agggattgct gttaagacag aaccgaggaa
    17401 tcctcgaatc actaagcgct ctgcttcttc tttatgcaat cctctactca tcagatagta
    17461 catttcttct ggatcaactc gtcctacact ggcagcatgt ccagcagtta cttcgttttc
    17521 atcgatcaaa agaataggat tcgcatcacc acgtgcttta tcagaaagca tcaagacacg
    17581 actttcttgt tgggcatctg caccttttgc ccctttcaag atgtggccga taccattaaa
    17641 ggtcaatgtt cctttttctc ggatcactcc gtgttgcaaa atatgcccga tcgtatgagg
    17701 ggctttattg gttactcgtg tatcgattcc ttggatttgt ctgcccgagc tgattgccac
    17761 gattttgact tctgcgtgtg caccttctcc tgctaaatct gagtcaaagt cagcgatcac
    17821 atgaccgtca ttcatgacac caattgccca gtcgacagaa gcatctcgta aaatgtgccc
    17881 acggcgattc atataagaag taatattttc tcctaattga tctacggctg aatatttgat
    17941 ttttgaacca gctttagcga tgacttccac gatgatatta cctgatgatt ttgctacttg
    18001 ttctttggtt gtctggaaac gttccagata agaaaattca ctatgttcat ctgccacgat
    18061 caacacatgc ttgaagaagt gctcatcgga tgcgccatct tgaatgaata aggactcgag
    18121 tggttcatcg atcacgacat ttttaggcac atacaggaaa ataccgctgt tcataaaagc
    18181 tacatggagt gcagtcaatt ggtcttcgtt catgtttact gcttttttca tataatattc
    18241 ttggactagt tctggatatt cgatcattgc agtgaacaag tcggtaaaaa tgactccttt
    18301 ttctgctaat tccacaggca gttgttcaaa aatcgtccat gaaccttgtt ggacaagcac
    18361 aggattgtct ttcattgcat caaatgccgg aatcgtgcct gtttcactga tggtttgtgt
    18421 ttctttcaca tcaaataacg accagcgatg gaatttcacc cgatcgatat gcggcaatgg
    18481 cagttcatct gctttcgcta gtgcgtcttg acggaaggtt ctcatccaat caggttcgcc
    18541 tttacttaaa gaaaaatcat ttaccgcgtc aagataatcg agccagtttt tttctttcat
    18601 tcttgctccc ccttacgctt cttcttcttt atagtcgatt cctaattctt tactgatacc
    18661 agcgtagcct tctgcttcca gacgtttagc taggtcagca ttccctgtca tgactactcg
    18721 gccttccatc atgatgtgaa cgacatcagg tgtgatatag ttcaataatc gttgatagtg
    18781 ggtgatgatc aatgcaccga aattgtctcc gcgcatttca tttactcctt tagcaacgac
    18841 tttcaaagca tcgatatcca gacctgaatc gatttcgtct aaaattgcaa atgtaggttc
    18901 taacatcaac aattgcagga tctcattccg tttcttttct ccgccagaaa aaccttcgtt
    18961 cagataacgt tcagccattt cttctggcat atttagtaaa gccatttttt catctaactt
    19021 tttcaaaaat gccataacag aaattttgtc gttttcatca cgtttcgcat tgatcgctgc
    19081 acgcatgaat tccgcattcg tgatacctgg tatctcactt ggatattgca tagctaaaaa
    19141 taagccaagt cgcgcacgtt catctacttc caagtctaaa acatttttcc cgtcaaacaa
    19201 aatttcgcct tctgttactt catagtttgg attgcccatg atagcggcag ataaggtaga
    19261 ttttcctgtg ccgttaggtc ccatgatggc atggatttct ccggttttca ttgttaggtt
    19321 tacgccttta aggatttctt tttcctcgat cgacacatgt agatttttta tttctaagac
    19381 agacatgcat tataccccca aattctcttt cgttctcact cattttagac taattgagaa
    19441 tgaagcgcaa tgaccttcct tactaaaatc aaatatcttt tatttattat taaacaattt
    19501 tgatttctaa attagaatag gtataaaaaa gatgagaaat aagccattac agacttattt
    19561 ctcatctttt ttgttccata ccttattttg catcagcctt tactggttta acagagccgc
    19621 cccatttgtc tgtgatccat tcttggactt ctttgcggtg aagcacttca atcagttttt
    19681 tgattttgtc actgtcttca tcacctttac gagtcgcaat aatattcacg taaggagagt
    19741 tgtccttttc aagcaatacg gcatcttcta atggattcaa ccctgcgccg taagcgaagt
    19801 ttgcgttgat tgcaactagg tcaccttcat cattttggta cgtagaagct aaaagtgatg
    19861 ggtcgatcgt atggttgaac ttcaagttct tagggttttc ttcaatatca tcgaatgttg
    19921 ctgtttccag atctactcca tctttgactt tgactaagcc agcatcttgt agaattgtga
    19981 tgatgcgtcc ccagtctgat tcagaatttg aagtaatgat tgttgcacca tcttttaatt
    20041 cggaaacatc tttgactttt ttagagtaca agcccattgg ttcgatgtgg acagcaccgg
    20101 cattgacaaa gtcatatcct ttttctttga tttctttgtt caaataagga atatgctgga
    20161 agtaattggc atcaatgtcg ccttcttcca aagattggtt cggtaataca tagtcatcga
    20221 atttgacgat ttcaagatcg attccttcat ctttcaataa tggtttcaca tgttccaaaa
    20281 tctctgcgtg cggtgtcggt gaagcgccga ctttcaatgt cgtgtcgtct gctttatttg
    20341 ctgaatcttt gctgtcgctg ttgttaccgc atgctgctaa tcctacagtc agcaaaagcg
    20401 ctgctgcaaa tccaaaaatt ttctttttca tatcctacat ccccctatat cctattatct
    20461 tttgtctgtt ttcttagtta agaaatcacc gattccttga acgataaata cgattagtaa
    20521 aataattaaa gtcgcaacga atgtcactgt tagattacca cgctggtagc cttcctgcca
    20581 agcaagacct ccaagtccac ctgcgccgat cgcgccggcc atcgcagtaa atccgatcat
    20641 cgagatcgtt gtaactgtta atccagaaat caatgcggga atactttcag gtatcaagac
    20701 cttccagatg atttcccagt aacttgctcc cattgcatct gctgcttcca gtacaccagg
    20761 gctgacttca cgaaaagcaa tctctactag acgggcataa aatggtgctg cggataaaac
    20821 aagagctgga atggctgctt gcgcacctag catagtgcct acgattgctt tagtaaatgg
    20881 aatgatcaaa accatcagga tcataaatgg cgttgaccgg aaaatattac tgatgatcga
    20941 tacgattcca taaatgattc gagcatacgg tttgtttttg cgtccaatag agtaaaggat
    21001 caatcccaaa actaatccga tgatcgtcac taagaccatt gagattaatg tcatatatag
    21061 tgtatcaagg atagcctgat tcatcgtttc cgggttgatt tgtttaaaat ctagatatgt
    21121 ttctgaaaag cttttatcca cgatggatca cctcaattcc tactccttgt tctttgatga
    21181 acacttctgc ttgatctaat tctttggttt caccagtcat caagacagtc aatgaaccaa
    21241 aggaaccttc tttcacttgt ttgatctttc catacacaac gttgacatcg atgttatagc
    21301 gtcgaatcgc ttgggaaatg actggctcgt tggcatttct ttcgctaaag gtcaaagttg
    21361 ccaaacggcc gcttggattt tctttgatga gttcagctaa tagttctgtc gaatcagctt
    21421 ctggttcgat gtcttgttgt acaaagcgtt tggttacttc ttttttcggt tgtctgaaga
    21481 cagtcaatac gtctccttct tctacgactt tccccaactc catcacagcg actttgttgc
    21541 agattttgcg gatcacattc atttcatgtg tgatcaagac gatggtcaaa ttcaatttct
    21601 tattgatcgc taacaataag tctaatactt cttctgttgt ttgcgggtcc aatgcgctag
    21661 tagcctcatc acaaagcagg atctcaggat catttgccaa tgctcgtgcg ataccgacgc
    21721 gttgtttctg tccgccggaa agttgtgaag gatatgcttg tccgcgtcct tctaacccga
    21781 ctaaacgcaa cagttcttct gcccgttctt tacgtttgct ctttggtacg ccagctaatt
    21841 ccagcggcag ctggatattt tcaagtacag ttcttgacca taacaggttg aaatgctgga
    21901 agatcatacc gattttcttt cggaatgtac gtaattcttt gttttttaat tttgtaatat
    21961 cttgatcgtt tacgatcact tcgccgctcg tgggaagctc aagtccattc aggagacgga
    22021 ctaatgtact ttttcctgcg ccagaatagc cgacgattcc ataaatatcc cctttttcaa
    22081 cgtttagtga aacgtcattg acggcttgga tgtttccgtt tttcccattg aatgtctttt
    22141 tgacattcgt tagttggatt aatgacatgc cattcacatc tttctcttat gatttttcaa
    22201 acaaaaaagc cttcgccctc ttgccttaag caaaaggacg aaagcttgaa cttccgtgtt
    22261 accaccttga tttaccgtta cttcgcaata acagcctctt ccagtacttg gacttagctt
    22321 ctttccgtat actgtcgcgc tgtaacgggc actcccgtag taagtttgct tacgctcgct
    22381 tactctgctc taagaccatc ttccatctca ttttcgttac ccattttcag caaacagggc
    22441 tctcttcaaa cgtccgtgac atgtactctt cttttcaaag ctttctcgtt tcatatagga
    22501 ttgcttatga ttatagcaac tggaagaatc agtgtcaact tttttcaagt gattttctga
    22561 ttttataatt cgtcaaattc ttttggatca acatctttga aacttaagat ccacgcattc
    22621 atttcatctg aatcattcaa gatattgata tcttcaccga ttttatcatt aacagaagaa
    22681 actgtacccg ttagcgggct ggcaaattca ttgaccgctt tttccgcttc aacttcgatc
    22741 agaggttcat ctttttttag cgattgtcct gcttttggca gattggcaaa cgtgatgtct
    22801 ccaagttctt cttgcgcttc atttgtcaaa ccaacacagt attcttttcc attaaataag
    22861 atccaaaggt tgtccttttt cttcaaatgc ttgcctgcca ttttatatca gttctccttc
    22921 ataaattttt tcattgaatc caattgcaac aagctggtcc tctcgtaaga tcagtggtcg
    22981 cttgatcagc ataccatctg tcgataagac ttggctcgct tttgaagccg taaactcgcc
    23041 tacttgatct ttcagtccaa gttcccgata tttcatcccg cttgtgttga acaggcgacg
    23101 gattggtact tgactcgttt ctagccaagt ctcaatcgtt gaggccttcg gtacttcttt
    23161 gatcatatca acggtttgat aactaacgcc atgctggtct aaccacgctt tggctttttt
    23221 gcatgttgaa catttcgggt accagtaaaa ggtgtacaat tttcttacct ccttgaagat
    23281 cttgcaggtg cttcattttg gtatctcatc gataatatca acccgatacc gatcatgttc
    23341 cctaaaatcg aggatcctcc ctgactaatg aaaggcaatg ggatccctgt taaaggaagt
    23401 aatccgatat ttgctccaat attctcaaag acatggaaca gcagcatcat gatcaaaccg
    23461 cttgcgatat aggcgtaaaa ttcattattc gtatcgaaac acacccgaat catacgatag
    23521 attaaaataa aatacaagaa aatgacgaat gcaccaccga taaagccgaa gttttcaccg
    23581 ataacggtaa aaatcatatc ggactcacga acaggcacat agatattgct tacgttgaat
    23641 cctttaccaa acaagccacc ggtaccaatt gccaaaagtc cttgggccgg ctgataactc
    23701 attcctgacg tatcatggaa agggtccagc catgaatcaa tacgtgcaaa ctgataagat
    23761 ttgaatccaa ctttataaag caagtctctt ccgccttcag tcgtaaccaa gaagatcgtt
    23821 cctgctccaa cgactactgc caagattact acaggtgcga tgatgcgcca agagattcca
    23881 gacatcagga aaatcccgcc aaagatcgca agaaagacaa gcatcgttcc aaagtcatct
    23941 tgggccatga tcaatgctaa aaccggtatt gtcacgatca agagcttccc aatcagccaa
    24001 aagtccgatt tcatcgtccg ttcttttact tgcgtattat gtttagttac gatcagtgcc
    24061 atcatcaaga tgtacgcaat cttcatcaac tctgctggct ggaaagtaaa cgcaccgaat
    24121 cgaaaccagt ttttagaccc tgttgaagca gctaaattct gatcatagaa tttgagcaag
    24181 agtcccatta ccactaaccc caaaccataa aggtaaggag tcagtttcca gatccatttg
    24241 gaattcaaat gcatgatgac aacgattgct gccgctccaa ctaaatacca gacaacttgc
    24301 atccctacgc ctttcaagac tgctggattg ttcggatcat ggctaagtgc tacatataat
    24361 gagagcagac caatcaaaca taacagaaag actggtaaaa tcacgccata atcaatacga
    24421 ttatcgttcg aagcttttaa ttttttttcc atgtttcatc ccttttctta aaaaccatac
    24481 tgctgattat tatagcggtc cagttagata atggaaagtc tttccataga catttgggaa
    24541 aaattaactt tccgtttgtg cagttgcttg agcaaatgca gtacaaaagc ctactgctta
    24601 tctttagtgt atcatagaac tttttttgat acatgcaatt atctaccagc tgagattctc
    24661 ctcgctgtga gaagtttctg tgctttcatt gtatcgaagg atctcttcgg caatctctag
    24721 cagctgatca gaaaccagat tggttcgttg gatgaaataa aaatatcgat ggaaattcaa
    24781 cggagtcatt ggcacatttt ctggaacaga gcgcttagac aaaatcgagc gaccgattcc
    24841 ttccttcagc aacgcaacaa ttacatcgtt acttttcacg atcatcttct tatcttggat
    24901 attcatctct tcaaaataac gctgcgtgta atgaaaaaca ccagagtttg cttctcttac
    24961 aagccacaat ttgctttttg ggtccccagc gatgactagc tggtcttcca tcaaagagta
    25021 tctttgtacg ccggaagtct ctaatggctt ttcgataaaa ccaaaatcgg attcatgttt
    25081 cgctacttgt tctaatgatt catgagaatt cgtgatttcg atctcaaaat gcactttagg
    25141 aaaccttttg atcagatgtt tcatcaactg aggcaaaaga tagacagcaa aggtgtggga
    25201 agcaacgatc cgacatactt cttcttcatt ttgttcattg agcaattttt gctgagtttc
    25261 ttcccattct tctaacaaaa ccagtgtccg ttcatataat aagtcggctt gcttagttgg
    25321 aagcatctct tttcttccgt tacgttgaaa aagatcgata ttcaattccc gctccaactg
    25381 tttgatttga ttcgagactg ctggttgaga taaaaacagt acttctgctg ctctggaaaa
    25441 atttcttgtt tcatatactg ctcgaaaagt cgttaataac ttaaacatgg caccacttcc
    25501 ataacttttt atgataatta ctataaaaac tatttgattt aaatatacaa aaaaccatct
    25561 ataatagaaa tgtacaacaa aacagttgct gatccaacta gtaacttaga tgaagacaat
    25621 cagctgtctg cttgctcgta atcatacatt cctattcatt ttacaatgta ttcgttcatc
    25681 ttggtactaa aatctattaa ataaaaagga gaatattata tatgaagaac tttttgaaaa
    25741 ttattccgat cccgatctgc gggttgatcc ttgggttagc ttcccttgga aatctgttta
    25801 aagactatca ccatgttgct ttaggcaatc taactggcgg tatctctatg attcttatga
    25861 tattcatttt tgttaaattg tttatcttat tcgaacacac gaaacaaacg ttgcatgatc
    25921 caatcatcgc ctccgtttca ccaactttta cgatgtcact catggttatt tgtacttatt
    25981 ttgtttcata tgaaagtatt gcaccaattg tcaaattcat ttggctgtgc gctgttatct
    26041 tccaagtgat ccttgtcttt tatttcaatt atcaccatgt tatcaaagct gatctttcaa
    26101 ttgaagcaat ttatccaagc tggtttatca tttacgttgg atttggggtc atcactgtaa
    26161 ctgccgggaa cttcttccca cttattggaa aaattttctt ttggatttca ttgatctgct
    26221 atgcgttctt attgccgatc atcatcaaac gtgtcttttt cgtaagaaat atggctcatc
    26281 cgacacgtcc gctgattacg atcgttgcag cacctggttc tctttgccta acaggttatc
    26341 ttaaaaactt tgctgaacct aatttttatc ttgtcgtagc attgttcgct ttatctcaag
    26401 tattgtattt cgttgtgctg tctatgcttc cacgtttgtt caagcttcgt ttttatccaa
    26461 gctatgcagc ctttacattc ccgcttgtta tctctgcaac agctatattt actactgttc
    26521 attacttcaa cagtttaggt atctatagta gcgtattgga tttcttaaaa gttgcggaat
    26581 gtctgtttgc aagtgtagtt gtcttctacg ttcttgctca ttacgtacgt ttcttaatta
    26641 aagaacatag caaacatgga aaatttgttt ctaaaaaaat cgcttaataa tcgattcaat
    26701 aggttgtgaa aaagctgttc tgcatcaagt aataagaacg gccaaacaaa aacagctgtt
    26761 catgtttttg tactggttgg gcttattacc gcagatgcaa gcttttgaac accgtttacc
    26821 aagaggtcgt aaaagaagtg gtcagcttca agaaataaga aggaatttac gaaaattgct
    26881 tctcaaattt ttgtgaaatt tcggcttatt tccgtagaag ctacttctgg aacaccgttt
    26941 attcgaaaca agaggtcgta caagactttt gtcacgacct cttgttcttt tgtcaaaata
    27001 ctggtttagt gctactatgt tcgagtatgc ttttagtatt tattgacgaa aggaaccgat
    27061 caaatgaaat cattgtcaca tccaataaaa tctgtaaagg attcagcggt ccgcgaaatc
    27121 ttacccggtt tactgctttc ttttgccatc gctgccttca gcaggcttct tgctatttat
    27181 ctgccacagt taggtggagc gactattgct attttattgg gtattttatt aggaaatact
    27241 ttttttaaac agcctgttct agcaatcggc acaaaatttg ctgaaagcaa actattagaa
    27301 ttctctgttt ttttactagg tgcaactgtc acagctcaaa cgattgctca aattggtgta
    27361 aaaggaattg tttttgtgtt actgcaaatg tcagtcacta tcatcggtgc ttacttgatt
    27421 ggtaaaaaac tgcaattcag cgatacttct tctttgatga tggctggagg aaacgctgtt
    27481 tgtggttctt ctgctattgc ttcaatcgcc ccttccatcc atgcgaaaga ggaagaaaaa
    27541 ggacagatca tcacactagt caatctactg ggtactgtga tgatgttgac tctccctttt
    27601 cttggattac agttgtttgg tggaaatctc attgaaaaaa gtgcactttt gggtggtaca
    27661 ctccaatctg ttggtcaagt agtcgctggg gcaagcttac ttgacaattc tgttgtcgaa
    27721 ttcgccatgc tatttaagat tctacgtatt atgatgctcg ttgttgttgt ctatctgttt
    27781 gaaaaattca tgcacaagaa acaaccagct ctgtttgaaa cgccaactga tacgcagaaa
    27841 aaacgaaaat taccgcttcc ttggtatgta ttagggtttg tgatcttttg tttggtcaac
    27901 agcttgtttt ctttacctgt agttattgac caatcggcac attttatcag cagctggttt
    27961 gagatcactg ctttagcggc aattggtctg cgtttagact ttgcaaaatt catgaaagaa
    28021 ggtacacgct tcttatctta tggattagct gtcgggcttc tccaaacagt gatggcttta
    28081 ctatttattt ggctgatcaa agtttagaat gaacatgaaa aaacagttgt gattctcgca
    28141 tggaacaatg tccgaatcac aactgttttt aattttgcag atatccttct aacaacaaca
    28201 attggcgttt gatttcaacc ccatctattg cgtttcccat atatcctgtc agttttccat
    28261 tttgcccgat cactcgatga caaggaataa cgattggcaa tggattggtt cgatttgcct
    28321 gcccaatcgc tcgcacagct ttcggactat caacagcaac ggctacttct ttatacgttc
    28381 ggatttctcc ataaggaatg gtcgacaaag ctttccagac attttgctga aaaggcgtac
    28441 ctctctttat agataaagga acatcaaatt cttttctttt tcccgcaaaa tattcttcaa
    28501 actgttcttg tgtgcgcgtt aaaattcgct gattcgctga ttcgctttct gaaattggct
    28561 gaaaagatac agcagtcaat ccggattcat ctgcatctaa ccataaaaag ccgataggtg
    28621 tgtgtatttt cattttgttt gctcctagtc aatagactgg aacttcctcc tttttctcta
    28681 tccaaaacta cttgccaact aataaaaatt gttcttaaaa gtttgataaa ccgaggaaac
    28741 atggtaaact aaatacgtta gtttacaaaa agaatacctg ttcttttacg taaaatcaaa
    28801 ttttaaaggt gttatttaag gtttattaaa ttttgaatgt aaaccatata taaacatacg
    28861 attgaaagga tagaaacgat gaaagtatca attgttgtcc catgttttaa tgaagaaaaa
    28921 actgttcctc ttttttttga tgaggttgaa aaattcaaag gtgcccatga gttcgaatat
    28981 ttgtttattg acgacggatc aaaagacggt acgttagcag caatcaagaa gctagcctca
    29041 gcttatcctt atgtccgctt tgtctcattt tccagaaatt ttgggaaaga agcggctctg
    29101 tacgctggat tgcaagcttc tacaggagat ttagtgaccg tgatggatgt tgatctgcaa
    29161 gatccgcccg aactgcttcc tgagatgatc aaccggatca ctacttctga tatcgattgt
    29221 gtcggcaccc ggcgttcgac tcgtgaaggc gagcctgcga tacgcagttt ctttgcgaaa
    29281 aaattctacc agttgatcaa taaaatcagc gatacagaaa tggtcgatgg cgctcgtgat
    29341 ttccgcttga tgactcgtca aatggtggat gccgtcctag aattaacaga atacaaccga
    29401 ttttcaaaag gattatttag ctgggtcgga tttaaaacag aatatatttc ttatgaaaac
    29461 cgcgaacgtg tggctggcga aacatcttgg tccttctgga aattattcaa ctactcgatt
    29521 gatggtatcg tgaacttctc ggatgcaccg ttaacgattg cttcatttat cggcgctttg
    29581 tcttgtgtcg gttctggtat tgcaattttg ttcattattt tgcgggcatt gttgtttggt
    29641 gatcctacat caggatggcc ttccatggtc tcgatcttcc tgttcattgg tggaatccaa
    29701 ttgttatgtc ttggaatcat cgggaaatat attgggaaaa tcttcttaga aacgaaacag
    29761 cgtcctgttt atatcataag agaaacagaa aaagtgaagc caaatgaatt gaagcctcac
    29821 caataatata cgtcaaatga agtaaacatt tttttaaatg aaaaaggact gtgacaaaag
    29881 tcttgtcaca gtcctttttt ccgaataaat ggtgttccag aagtagcttc ttcggaaata
    29941 agccgaaatt tcacaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa
    30001 gctgaccact tctatcacaa cctctttcat atcttattac ttcttcattg ctgcttttag
    30061 tgctgctgct aatgggcttt cttctggttc ttctgcttgt gcatattttt tcagcaagcg
    30121 tcgttcttca tgtttggtca ttttcttctt ctgctctttt ttatcaagca atttttctgt
    30181 cgttccatcg tatttacaac ggaaataagc accgttttta ccttcgatga tttccatttt
    30241 ccggtgacat tgcgggcatc gatgattaga gacttttgga tcttttcttc tgcggtagtt
    30301 gcaatctgaa ttggtgcaga catagatttt gccgtcacgt gtgtttttct cgcgcagatt
    30361 ggccccacat tctgggcatt tcttttgggt aatcgagaaa tcttgatatt cttgtttgct
    30421 tgatttgatc tcattcacca agcgttttgt ttctttttcg attccattca agaaggttcc
    30481 tgcttgtttc tttcctgcag caatagcttc aaggtctttt tcccagcttt ctgtcaaatc
    30541 tggtgttact aaagagggat ttaccaatac taataattgc ttacctttag gagatacttg
    30601 cagtccttga ggggtacgtt ccatcagttc agatttgatt agtttctcga taatctctgc
    30661 acgcgtagct ggggttccca gactgtattt ttccatttga ccaagcaagc ttgcttctga
    30721 caatggttta ggcggtgcag atagttcttt tttgatggcg aaatcgggat tgattctcat
    30781 tcctttttcc catttgactg tttccgtctc agtttcagtt tcgcctttcc atcctgcttg
    30841 gacgacacga ttttgccgaa aaatgaatcg ttctttacca aattcaaccg taacttttgt
    30901 ttggctgact ttatggggtt gagcaaacag tcctaaaaaa cgagagacaa tcatttgata
    30961 gatttttcgc tcttcattac ttaatttttc ataacgtgct ctgttttcag ttggcaaaag
    31021 tgcatggtga tcggtcactt tactgtcttg gaatactttt gtctgttgga cgatggctcc
    31081 gtttttcaaa tactgcttga cctcaggagc aaagtcgctg actgcttgca atcgttcttt
    31141 catcgtactt ttcatgtcat ttgtcagata tttgctgtct gtacgcggat aagtaacgat
    31201 cttatgggtc tcatacaaac tttgtacaag acttaatgtt ttctttgcag aatagccata
    31261 gcgttgattg gcttctcttt ggatctctgt taaatcataa ggcaatggcg cagattccgt
    31321 ttttaatttt tcttggactt ctgtgacagt tcctttgctt gtactgatca attgaacaag
    31381 tttttgtgct tcttcttgtg tttttaaagc aaattgattt ttttgaagca ttttggcttg
    31441 ttcttttcca gcttgcaacg tgatcgtgaa atacgtttgt ggacggaact gctcgatttt
    31501 cttttcttgg tctctcacta atgctagtgt cggtgtctgc acacggcctg ctgataaatt
    31561 atccgcgtac ttcacagtta aggctctggt cacattcaat cctaccagcc agtccgcttt
    31621 tgcccgggca agagcagatt catacaatgc atcatattct ttcgcaggtc ttagttgctt
    31681 gaatccagtt ttgattgctt tatctgtttg agaagagatc cataaccgct tcactggttt
    31741 attgaaatgc acatactcta agatccagcg agcaacaagt tccccttctc ttccggcatc
    31801 tgtcgcaatg actgcttctg agacatcttt tcgctgagcc agctgcttga ttgcttttaa
    31861 ttgatgtcct gttttaggaa gtggtttgat tcctagtttt ttagggatca ttggcaacgt
    31921 gtccatttgc cagctctgcc attccttatt cagatcttcc ggcattttca atcctaataa
    31981 atgaccaagg gcccaagtaa cgatcacctt gggtccttca taataatttt tgtgcttttg
    32041 attagcacca agcaccttgc ttaggtcttt cgcaacgctt ggtttttccg ctagaattag
    32101 ttgtttcata aaattccttt ctgctttcta ctgtgatggg ctgatgattt ttcaacaacg
    32161 caagtccctt gtattgctgt aaatcatcgt cacactcttc acaccagcgc tcatctccgt
    32221 cattccaaaa ggcgctgaga aagttacttt ttgcattgat atattcataa ttttgatgga
    32281 tttcgttcca ttgtttttcg tacgcttgtc gtgcttcttc gaaagactcg aatgtttctt
    32341 cttcttcaat atcttcttcc cagtcttcaa aaaaccacca aggctcattg tccccgaaca
    32401 tggtaattac ttgatacata ctcgtgctcc ttctttcagc ttttctagac tcatcttagt
    32461 cacttttcaa gaaagttgca agaattttct cttataaaag ggtcatttaa tgaacatctt
    32521 aaaattgtta caaatgaatc tgattgttta aggcaaagta gcgataaagt tattgatcat
    32581 ccaaaaatag ctgtcttctg gatcgattgt tggttctttt gtaaagtatc ctaaaaaacg
    32641 taaggaaaga taaccgtgga tcatactacg aagttccctg cttcgatgaa ctttttcttc
    32701 tttcgttaat tgaaaagcct caaatatctg caaaatgatc tgattcgttt catggattcc
    32761 atctaataaa acagcttcat tggtatgcgg tacactgatc agtaactcat acactgcttg
    32821 atttgaaaaa gcaaaggatc gataagtttc tgcatagatc tgcagtgctt cctgaccact
    32881 ttttcccaca agagcccgtc gcagctggtc attaagttcc tgcagaagtt tcgctgttag
    32941 cgctgttttg acatcacgaa tatttttgaa atggttatac agcgagggag ccttgatatc
    33001 caagcgtttg gctaaatgct gaaaggttat ttgctgaaca tccatctctt ctgctagttc
    33061 tcgaaagcag tcgatgattt tctcttgaga cagatttctt tttttcattt ccatgtttcc
    33121 tctcataaaa taaatccttc actctttcag tttaccacga aaaagaaaga gaaaaaagag
    33181 actttcattg ataaactaca tattgtagtt tataataaag agctgtagat gaaaattttc
    33241 agaaagaagg agtctaattg gctgcaagaa taaaggaaaa attatttagg aaaagagaaa
    33301 cttgggaaca aaacttgttt gtcttatggt ttggaacatt tatggctggt atcggattta
    33361 gtttagtcat gccttttatg tcactttaca tagatacgtt aggtaattat agtacgtcac
    33421 aattgaactt ttggagcgga ttgacttttt catctacttt cttggtgact acgcttatct
    33481 ctccttggtg gggaaaatta gccgaccaaa aaggaagaaa attgatgctg ttgcgtgctt
    33541 cattaggaat ggcaatcgtc atttctttga tgggttgcgt gactagtgtg tatcaattag
    33601 tcgcattgcg tctgcttcaa gggatttttt ctggttatat cagtaatgcc actgcccttg
    33661 tcgcaactgg gacgccaaaa gaaaaaagtg gtcaagttct tggaacctta gcaactggtt
    33721 ctgttacagg aacattactt ggaccgcttc ttggcgggat ctctgcttca gcgtttggct
    33781 atcgccctac ctttttcatt acagggatca tcttattttt cgtcttcttg ttgagtctgt
    33841 ttttcgtcca tgaaaaattt gtcccagtga aaaaagaaaa catggtttct gccaaacaga
    33901 ttttcaaaga cttgaaatat ccacacgtcg tcatcggtat gttcgtcaca acgatgatca
    33961 tccaagcatc caacaattcc attagtccaa tcatcagtct gtacattcgc caattgctgc
    34021 atggacatgg gaatgttacg ttgatcagcg gaatcattgc ttcgatacct gggattgcca
    34081 ctttgatagc cgctccaaga tttggtcgct taggtgacaa gattgggagt gaaagaatac
    34141 ttgcgattgg tttgggtttt gcgattctgg tttatatccc aatggcattc gtcacgaatg
    34201 tttggcaatt agctgcctta cgttttttga ttggtatctc tgatgcttgt ctgctccctg
    34261 ctgtccaagc attgatcaca aaatattcgc ctcatcaagc tgctggacgg atcttcagtt
    34321 ataaccaatc tttccaagca actggtaacg tagtagggcc tatgatcggc tccagtgttt
    34381 ccagtgcatt tggttatcgt ggcgtctttc tttcgacttc tcttcttgtt ttggtcaact
    34441 tcttgcttgt acgacacaat acacaagaga tccatcagca agaaactgca gaagaaaaag
    34501 cttcaggagt ctccatagct tcagggcact ctccttctca tagacaataa gcgtaaatga
    34561 aaaacactgc ttgaacagtt gtcacagact gtccttgcag tgtttttgtt taggcctgtt
    34621 ttttcaagaa ctcagataag aggaataatc ccttttccaa ggtttctttt ttacaacagt
    34681 aacctagtcg ggcatgcttc ggtaaatcaa aggcacttcc tggtactaat agtactcctg
    34741 tttcttctaa taaattgata caaaatgtct ggtcatcttc ttcgatggct aatttaatga
    34801 aagaagtaga gacgtaattg ggagcgacaa gttcaacttt cggttcattg gctacccatt
    34861 gttttaaaat cacaaggttc tctgtaatga tctttttgtt tcgcgccaat attttttctt
    34921 tattcttcaa tgcatgtaca gcaagatcat ctgacaggac tccgccgcaa atcatcgtgt
    34981 agtctcgata ctttcgaaaa acttctgcca actcaggtcc agtagctgtc cagccaatac
    35041 gaatacctgg aatggagtag gttttggata gggaattggt tgctatacct ttttcataga
    35101 catctacgat cgacaagaat tcacctttat ccgttaaagg cgcatatact tcatctatca
    35161 atacatatgc atctactgta cgagcaattt ctgcgatttt ttctaaagtt ttccgatcta
    35221 gcaatgtgcc tgtaggattg tttgcactat tcaaacagat gatttttgtt tcaggtttga
    35281 ctaaggcttc tagctgttct aaatcaaact gccaatcttc ttcttccttc aaatgaacaa
    35341 agtcaacagc ggcacctaaa gatttgggga tgtcatataa ttgctgataa gtaggaagca
    35401 tcgaaacaac atggtctcct gcttccacta acgcgtatag agctaaaaga ttagcaccag
    35461 tcgctccatt cgtttgtaag atgttttccg gatcgacggt ttgataaaga gaagcgactt
    35521 cttgtttgaa aacatctgat ccttcgatcc agccatagtc caatggttcg tttttgcgtt
    35581 tttcgaaaaa tttacttacg ctagtcccat ctaagccaac gagttcgtct aatgtcaatg
    35641 cttcgatcga gctttgtgca atatcatata ccgctttctt ttcccattta tttagccatt
    35701 cttctacacc gaaatttgcg attttcatgt ttctttcccc cttatatctt gatccagcct
    35761 acagcaagca acccgattga aagcagtgcg gccacacaaa tcagcccaat cattactttt
    35821 tcttttcccg taatttgatg tttggtttca cgacaagcga tccagtagaa gcaaaaacca
    35881 ggaagatagg caatactaca taaaaggaca taagaaaatc ctgctaaaat gatgcccatc
    35941 aattcaaaaa ctacagcaat cccaccgatc actacttgtt ttttgattcc ttcttttaaa
    36001 gaaaactgga cttgatagat cccgacaagt agataacaaa tcaaaattgc cgctgtacat
    36061 aaagaatagg caaattgata ggcataatca gtaactaaga aagtcaataa gaatagatta
    36121 gtcaagattg cagtaacaac taacgaataa gttggggcat tttttttatt cgtttttccc
    36181 caacctttcg gcaacagacc gtctcgagcc atcaacatcg ttgtttctgc tgggagcatc
    36241 gtccaagaca accaagaacc aatgatcgaa ataatcaagc cgatattgat cagcgctgct
    36301 ccccaatttc ccacgattcc ttttaaaata tttgccatag caggttgtga aatagttgct
    36361 aactcttctt gagataagac gccgtatggc aagatcgaag caaggatata gataaacaat
    36421 aaacaaagca gaccaatgat cgatgcttgc tgggcttctg aacgtttctt cgcgcggttg
    36481 gctaaaacac ttgcgccttc gatccctaca aagacccaca tcataaccat caaacaacct
    36541 ttaatctgca accagatcgt tccttgctgc cctgttccat tcactagatt atcagatacg
    36601 ttaccccaaa aatcagcagt gaatatattc actttaaatg cgataaatac aaatattaaa
    36661 aataggaata atggcactaa tttacagatc gtcacgattg tattgatgac gctcgctcct
    36721 tcaactccat tgtttactaa taaagtcaga ccccaaataa aaatacttgc aaataaaata
    36781 gaaggaacat tttgtccacc tttgaatatc gggaaaaatt ctcccatggc gctcatcagc
    36841 atcgttgcaa aagcaacatt tcccagccac gcagacaacc agtaacccca accactgatg
    36901 aagccaccta atttaccaaa tgaagcttcc gcgtaaccga aaataccacc atctagatct
    36961 ggacgctttt ctcctaaatg attcagcgat aataccaaag ctaaaatacc aatcccaacg
    37021 attatccatg aaaggatcgc tggccctgga gctgcagaac tagccagatc acttgtgatc
    37081 ccaaacacac cgcttccgat tgacgaactg acaaccagag caaccagtcc ccatttgcta
    37141 atacccgtcg atttcttttc catttccatc accctcactt ataaaattga acaaatcact
    37201 tgcaaaagat tcccttctat tttgcaactg ctcttgctta acaaaaagca actttattca
    37261 tattattgga tattatgaac tttttacaca agtatttcaa gtataaaatg aataaaaatt
    37321 aattttattg caaaaaaaca gctatgctat ccaaaagaaa aggtgtttct tttgttagcc
    37381 atagctgttt tgattcattt aatatcgctt aaacgtattg taaaactttt gtttcttgat
    37441 atgcgtggtc gatcaaacca ccacccagac attccattcc atcataaaat acaactgctt
    37501 gtccaggagt gattgcacgt acaggttcgt cgaatacgac ttctgcacga ttatcatcca
    37561 ataagcgaac tgtcacagga acgtcttgct gacgataacg gaatttcgct gtacacttga
    37621 attcttttgg cattggttca ttcgtcgtaa agtggatttc acttgcatcc aagcttgtgg
    37681 catacaatgc aggatgatgg aacccttgcc ccacatataa tgtgtttgtc gctagatctt
    37741 ttcctacaac aaaccagggt tcttgcgaat ctcctccgcc accgatccct agtccttgac
    37801 gttgaccaat cgtataatac atcaagccag catgttgtcc tttgacttca ccatcaagag
    37861 tgaccatatt tccttttttt gctggcagat agttgctcaa aaattgtttg aagttctttt
    37921 caccaataaa gcagattccc gttgaatctt ttttcttagc agtcgcaagg cctgcgcgtt
    37981 cagcaattgc ccgtacttct gatttttcca tgccgcctag agggaacatc gtttttgaaa
    38041 gctgttcttg tgataattgg ctaaggaagt aagtttgatc tttattattg tctactccac
    38101 gaagcatgtg gaccacgccg ttttcatcgc gagtgacttg cgcataatgt ccagtagcta
    38161 cataatcggc gccaagatcc attgcataat ccaagaatgc tttgaatttg atttctttgt
    38221 tgcacatcac gtctgggtta ggtgtacgtc ctgcgcggta ttcggctagg aaatattcaa
    38281 atacacgatc ccaatattct ttttcaaaat tcacagagta ataaggaatc ccgatttgag
    38341 cagctacttt cgctacatcc ttataatctt ccgtggccgt gcatacaccg ttttcgtccg
    38401 tgtcatccca atttttcatg aatattccga tcacgtcgta tccttgttcc tttaacaaaa
    38461 gtgctgttac agatgagtcg acaccgccgc tcatccctac gacaacgcgt gttttgctgt
    38521 tgtctgtcat gatatcacca tcatttcaat tagattctga aacatctacg agttgaaggt
    38581 cgcaactttt cagtatttta cattatacgt catatttctg aaaatttcat cccttaatta
    38641 tcaagtcgta aaaatactgt tctactggac ctattcagta cacaaacatg atagtcaacc
    38701 aaaaaatcac tcatcttttg agcattcgat ataaaaaatt ggcatactca taaaaagggc
    38761 cagcctttcg gcaataagga ataattttat aaattgtgaa aaactatttt tcaaaattgc
    38821 ttcttatcgc ttaaagctgc acgtctcatt tacaaccttt tctttttaga tgttatgagt
    38881 tgctcagata agtgattttg tttactctaa gaaaaaaggg aggcaaaaga tacatgaatt
    38941 actttatcag tgatatgcac tttttccatg aacacttatt aggaaaaaac gactttgctc
    39001 cgcggctctt tgcaaatgtg gatgaaatga accaacagtt gatcactagc tggaatgcag
    39061 taataaaaga aaccgaccat gtttatcatc taggcgacct tgctatgcat ccgcaatatg
    39121 aaaaagcaaa tgcagaaatt ttggaactgg tcactcaact gcaaggaaca atccacttga
    39181 tcaaagggaa ccatgacagt cgcgcttttt tccgttattt agaaaaaaat gatcctaggc
    39241 ttactgacgg aagtcccaaa ttttattttt atgatgtagg tgccatcgtt aaattcgacc
    39301 atcaacaata ttacttgact cattatccga tgttgctcgg gcctaacgac actatccgca
    39361 atcttcatgg acatatccat cattacagcg tacctatcga gaacgatgtc aatgtaggcg
    39421 tagacgcacc ggaaagagaa ctcttaaagg aaaaactacc atttggcact ccgttatctg
    39481 agcaacaaat caaagaaatc tatgaagcaa agaggaagta cgataggtcg tgaaaaaact
    39541 gccctatatc aagtattaaa caaataaaga taattactca tatttttcat ttgagtaggc
    39601 ttattgccac aggaacatta ggatcagaaa tggagttgta acaagacttc tgtcacaact
    39661 ccattccata ctattcgtct ttttgctcat ggtgttcttt taccagtttt ttctgtaatt
    39721 ctttatcata atttcgggca gctgacgtta aaaataaaac gattccgcat aataaaatcc
    39781 cgattccgga aaacaagaac attttttcta cccctaaacg atcagctaat ggtcctgcaa
    39841 ataataatcc aattggacct gccaggttca tcaaagagtt gaagttccct aagacacgac
    39901 ctaaaacatt cgattcataa ctctgctgga tcattgccat cagcagtgta ttgaaatatg
    39961 gcgttgcaca acctgctcct gcattcaaga taagaaagta caagaaccct tgactatcgt
    40021 ttggaaccaa tccgcttgca ccgatcgtca ggcccataat gatgtaggct ataataatag
    40081 gtttcattcg gtctttccat gttcctataa aactgatcag tgcaccgcct aagagcattc
    40141 cgactgcgta gactacttcc actgctcccg cctgtccaac ggaaccacca aagtaatcta
    40201 gcgtcatcaa tggatacaga ctgattgctg gcatgaacaa cagcatcaca aatgcaccat
    40261 tgatcgttat gtaccataac cctttattat ccatcaattg ctgcagccca aattttgcat
    40321 ctttaaaaat agtaagcgga acttccaata tttcaggaga aacttttggg atcttcacaa
    40381 aaatcaataa gccgacacca aaaactgccc ccaatacatc taataaaata aggtaattga
    40441 caggaacgac tgaaaacaaa gccgcaccta gagctggcgc aatgatgaaa ttcgctgatt
    40501 ggaccattcc taactgtcca ttcgtccttg ttatgtgaga agatggaacg attgtcggca
    40561 taatcgactg gatcgttggc atttggaatg tttgtgcgac cgaacgcata aacaaagaca
    40621 caaaaactag ccaaatcggg aaggattctg aaatcgttcc gactactgct aaaataagcg
    40681 cgaaaatcgc tacaataata tccgtgacaa tcaaaagagc ttttttatcc cagcgatcca
    40741 ctagcggtcc tacaaatggg cttagtaaaa ccatgggaat cattcctagt aaagtagcaa
    40801 aactcaaaat tgtcgccgaa ccggtttctc ttgtcaaata ccagatgatc gcatattgga
    40861 cgaccatact agtgattcct gacaaaaact gtcccgataa aaagaacaaa taatttctct
    40921 tccaattcgt taaaacgacc gactgttcat tttccataaa catccctctt ttctttttca
    40981 ttttgtatag cgtttgcaca acaatcatta tacgggattc ttttttcaaa aaataccttt
    41041 ttccctaaat aacaatcgtt tcaatttaaa tttctttact tagaaacaat aaatcagcaa
    41101 aatattaaaa ataacaacat gtgttaaatt taaaaataaa aaagacaact aatttgcgtc
    41161 gtccttacaa agttagtcat ctaacaatag gccgtttcaa aactagttgt ctttttatat
    41221 ccacagatca ttcgactttt tcgaataacc cacggctaat catcccatcc atcagaaaat
    41281 aaaatttatc ttggctcatt ttctgttttg gatctttgaa aatattgact gctttcaatc
    41341 catttgctgt agtgatcgac attttcaatg tttgaatatc ttttgataca gtgattttta
    41401 atttgaatcc ttctttgctt tgcggcgtgg catctaatgg tcggatcaat tgataattgc
    41461 ctccgtttgt ttctgtaaat ccattatcgc gcaatgtata tctttttgca tcaggatgca
    41521 aacgatagct aattggtgcg cccagtaccg ttgctgttgt ttcaaatgcc atgattttca
    41581 cctcgataaa attagtttct ctttattata aaagtttatc ctgattttta ctaggttttt
    41641 cagttaagtt ccctaaaaaa tgaaggcgtt ctctcagaca tctgttcata taagaatgac
    41701 ttcactagtt attttttgac agcacgaact aattgatcaa ggaaatactg gatgtcgtct
    41761 tcggtatttc ccaaaccaaa gctgatacgg atggattctt ttagtatcgg tgtatcttct
    41821 ccatacattg cttctaatac gtgtgaaggc tcgatatttc ctgctgtaca agctgaacca
    41881 gtagaaatag cgaccccatt aagatcaagc ttcatcagta gcaaatcatt tgcttcgcca
    41941 attaagcgaa gattcaacac atgagggagt ttattcgtta aatcaccatt tacttgataa
    42001 gggatttcgt ttgcttctaa tgtatcaaga atcaattgct ggaatccttt atatttgttg
    42061 ttacgtgctt cacgttcttc aggtgacagc agttcaactg cttttgccat accccagatc
    42121 cctgctaaat tttctgtacc tgcacgccgc ttttcttcct gctccccgcc taaaagcaat
    42181 gcagggatat ttactccgtc tcgtttatat aagaaaccga cgccttttgg cccattgatt
    42241 ttatgagcag agatacttaa taaatcgatt cctaattctt ctggatggat cgtttggttt
    42301 ccgtacgctt gtaccgcatc tgtatggaaa taagctggat gatccgctaa aagttcaccg
    42361 atttctttga ttggcatcaa atttccgatt tcattgttcc cgtacatcac agagaccaag
    42421 atcgtatctt ctcgtaacgc ttttttgacc tcttcgatcg acagattccc tttttcatct
    42481 acaggcagat atgtaacgtc aaaccccttt gtctctaaat atttcatggt attaagaaca
    42541 gcaggatgtt cgatagctgt agtgattaga tgtctgcctt ctttttgacg cgagaacgct
    42601 gtttctaaga tagctgtatt atccccttct gttcccccac tgttaaagat gatctcgtga
    42661 ggttttgctt gcagactgtc tgcaatgatt tgtcttgctg tttccaattt ttcatgcgcg
    42721 tggcgaccaa atccatgtat gcttgaagga tttccaaacg tgtcttgcat gattgccgtc
    42781 atcgcttcaa ttacttgtgg atgcatgggg gtagttgccg catgatccag ataaatagtt
    42841 ttcacttcat tcatcccttc caaattattg ctaggcacat tctagcataa aatagcgaac
    42901 aaaccaaaga agagctcatt tttgtctaga ggacgtttca tgggcataga aaaaaggagc
    42961 tgtgacaaaa gtcttgtcgc agctccttga agctgctcac ttctgtcaca acttctttcc
    43021 ctcttttttt tgtttattcg aattcttttt cttccagacg gaataacgga ctcatcggtg
    43081 tattttcatg gatgcgtttg accgcttcac ccattaattc tgaacatgta acgattgtta
    43141 agttttctgg atgacgattt tcttctgtga aaacagaatc agtgatacaa atatctttga
    43201 ttggtgcatt gtctaaaata cttttcgcat gatcggacaa taatccatgt gacgcacagg
    43261 catatacttc ttttgcgccg ttttctttta atacatttgc tgctcgtgca agcgttgctc
    43321 ctgtgttcaa gatatcatct accatgatac atgtctttcc ttgaacatca ccgatcacat
    43381 aaccaccctc agttgaagca tcatctgcat gatctacgat ggctaatgtg ctgttcaaat
    43441 attctgaaag actacgtgca cgctgaacac cgctgttttt tggcgaaaca actacgatgt
    43501 catctcccgt cagacctaat tgacggtaat aatgagcaaa caacggcatc gtaaataaat
    43561 tatctacagg aatatcaaag aatccttgta cttgaaccgt gtgcagatct aaagtcagta
    43621 ctcgagttgc ccctgcttct tcgatcaagt tagcgatcaa tttcgcagtg atcggttcat
    43681 gaggtttcgc cgtgcggtct tgcctagcat acccatagta aggcaatacg acattgatcg
    43741 ttttcgcact tgctcgcttc attgcatcga tcatgatcag tagttccata tagtaatcat
    43801 tgacaggtgc attcgttgat tgaacgatat acacatgatc ccctcgaata ctttcttcga
    43861 tattgatgga aatctcgcca tcactaaatt gcttcacgac gcttttacct aattctgttc
    43921 cgaacacttt cgcgattttt tcagctaatg gacggttgcc attcaaacta aaaatcttca
    43981 acgtgtcatc ctgatactta ttactcatgt agtcctcccc aaaacttctc tattcttcga
    44041 tagtttaaca tatttttcca agattttctg ttaatttcag caaattctta aggaagtgaa
    44101 actgttccgt tactccaact ggcaggaatg actttatgat aatcgcccca gttgtcgtgt
    44161 actttgtaat aacgttctcc tgtttcagtt tcaaaataag cgtacgctgt cacccaatga
    44221 ttgccatatg tactgccaag tactttcaaa ataccaatca tcacaggttt gccttcacgt
    44281 atacgttttg tagctcgctg ccatgaacct agaagagttg ctcgtgcacg ctcatgattg
    44341 ccattcttct ttaaaaaact agaaatccct gtactaacct gaaaaggaat cgtaggaagt
    44401 cctagtggtt ggatctggct acgcaatgcc ttcgtcaaaa ccaacgaatc agccgtattt
    44461 ttcttgcgaa tctcatttgg aagcatccac cccttacgat agtcttgata ataggcaagg
    44521 agaactgctg ccgcgtaagt accacaaaga tagcctgagg gggtagccca tttcttatac
    44581 ctcaaaaaac gttcttctgg caatcccacc cagcaatttg cgagttcctt tcctgacctt
    44641 ttttcttcga tcaaaacggg aatatcttct tttctgttga tccatctaga taactcgatt
    44701 ggctcttcat tgatcagctt ccatttttta caagaaagac cctcttgata agtatatttt
    44761 gctggagaat attctaccgg atgtcctgat tgatcgataa tcacataact ttgatcttca
    44821 aaaaccagag ctaaaaaaag caatttactt tttcttgact gcagctgctg agtgctggct
    44881 aattttttaa tttttttatc tactttgaaa tataaaggtt tatccactac ttgattcagt
    44941 tgggatcgcc tccttttttt cattataagt gaaggattga aaaaaacaaa tcagtttgat
    45001 tgttcgaatt ttcaattttt tatgttctat ttgttaattc cttcattttg taagataatg
    45061 ggcacacgaa acaaaaaagg agcgatcgat gaatgaacaa agaacgattg aaacaagatt
    45121 tctatgaagc agtcaatgaa gaatggcaaa aaacagcaaa aatccctgca gataaaccag
    45181 ctacaggcgg tttccaagat ttagttgacg ggatcgacca acttttgatg ggagaaacag
    45241 acgatctgct tgcccatcca gaaaaagtgt cttcagaaaa aatgaaacat ttccttgcat
    45301 attatcaact ggcaaacgat tatgaccgaa gaaatgagtt gcatgctcaa ccgcttcaac
    45361 ctataatcga aaaagttgaa tcattgcagt ctttcgacga attgaataag caatttccag
    45421 aatggctgtt ggatacatta ccattgccat ttaatctgga tgtagatgca gatatgaaga
    45481 atgcgcaaac gaacgccttc tttgcttatc cgccttcttt attccttcca gataagacgt
    45541 attatgcatc agaccatccc aacggtgcac aactaatagg cgttttcttt gatatggctg
    45601 ttcagttatt agagcttgcc ggcaaagaaa aaagtcaggc agaagcaatc acggaacaag
    45661 cagtgcaatt tgataaattg attgctcctc atgtaaaaag tgcagaagaa aacgcagatt
    45721 acagcaaaat gtataatccg caatcttttg atacatttat taaatacaca gataaactgg
    45781 acttgaaaaa acttgtcacg ggtttgattg ggacaactcc agataaagtg atcgtaactg
    45841 atccagtgta cttcgatcat ttgaatgaca tcctgacaga agctcatttt ccgttattca
    45901 aaagctggat gatcattaaa acagtacgtt ctcttagtgg ttatctatca gaagaattcc
    45961 gccaagtcag cggaatctat tctcgcgtat tgtctggaac agacgaagca atgcctcaga
    46021 aaaaagcagc gtattacctt gcctctggac aattcgatca agtagttggc gattactacg
    46081 gaaaaaaata ttttggcgaa gctgctaaaa aagacgtaga acaaatggtg aaaaaaatga
    46141 tcgccgttta taaaaaacgt ctagaagaaa atgactggct tggggatgca acaaaagaaa
    46201 aagccgtcat aaaattaaat aacttaggta tccaagtagg ctaccccgat aagatcccag
    46261 cattattcga ccagttcaaa acagtacctg cagatcaggg tggtactttg ctttcaaata
    46321 ctcttgtttt cagcaaactc acattaaaag atcgtttcag caaatggaac aaaccagtgg
    46381 atcgttcaga atgggaaatg agtgctgaca cggtcaatgc ttattatcat ccatttcgga
    46441 atgtgatcgt ctttccggca gcgatccttc aagcaccatt ttacagtcta gaacaatcaa
    46501 gctctgctaa ttatggtgga atcggagctg ttatcgctca tgagatttcc catgcatttg
    46561 ataataacgg cgctttattt gacgaatatg gcaacttgaa caactggtgg acagaagaag
    46621 atcttacaca atttcaaaac aaagcacaag caatgatcga agaattcgat ggtctgcctt
    46681 ttgctgacgg aaaagtaaac ggaaaattaa ccgtttcgga aaatattgct gatgcaggcg
    46741 gtctaagctg tgcattggaa gcagcaaaaa cggaatcaga tctttctatc gaggaattct
    46801 ttgtcaattg ggcaacgatt tggcgtacaa aagcaaaaaa agaataccaa caacttttac
    46861 tgcaaatcga tgtccatgcc cctgctaaac tccgagcaaa cgtgcaacca aaaaaccttg
    46921 acgaattcta tgaaacattt ggtatcacgg aacaagacgc tatgtatcta gcgccagaaa
    46981 aaagagtgcg aatctggtaa gaggttgtgc cagaagtggt cagcttcaag aaataagaag
    47041 gaatttggga aaatcgcttc tcaaattttt gtgacatttc ggcttatttt tcgaagaagt
    47101 tacttctgga ataccatgta ttagaggttg tgaaaaagct gcttagtttc aagtagtaag
    47161 aaaaattttg tgaaaatggc tttccacatt tttgcataaa tttgacttac taccgagaaa
    47221 ctggcttttg aacaccgttt atcgagaggt tgtgaaatca gcgtcctgac ctgagtatta
    47281 gagaaaaaaa cggaaaaata gttcctcata ttttgccgtt tgttgagcta ataccgaggg
    47341 tcagctgatt gaacaccgtt tagtggagag attgtgaaaa agctgtcctg catcaagtaa
    47401 taagaacagc caatcaaaag cagcaaggag aacctcatct ataaaaagat agaaggaagt
    47461 gagacatcta ttggtctcat ttccttctat ttttactttc ctcagtcaat tgttttacat
    47521 cataaaagga gaaattccac ttattttttt cgctgtttca tgacataaac aactacaccg
    47581 attaacaaaa ttcctccagc taatgctagc aacgggttat taaattctcc cgtttttggg
    47641 agtccttgag tgccattttt ttcactttct tttgctgtcc tatccggatg agaaggtttt
    47701 ccatttggtt ttccatttgg tgattcattt cctggatttt ctctttctgg tttgtcattt
    47761 cccgggggca catcggcagt ttctcgcaca gtgatcgctt tttgctgggt aacagattct
    47821 gtatgagcag accatttttc cccatcaaat atttccgaac gataagtgac ttctagcgta
    47881 tattctcctt ttgaaggtgc tgaaaaaata gcggtatgat tagaaaattg agcacttgac
    47941 tgtgtttcaa cgatttggat cttttctggg aaataacgca tatcttcaaa gacaggcgtt
    48001 ccttgatttg gtgtgaaagt tgacacaaaa actgtaaaag gcttcagttc aatgatattt
    48061 ccttctgcat tctccgccac accatttgct tcttgtataa taatcggatt attttgttca
    48121 tttggttttg tctgatttgg ttcttgtacg attgcatact ctggatattc cgaagacggt
    48181 gaggtttctc cgtcaaaaat agcacctaca gataatggaa gaccatccac tctttccttt
    48241 tctgtttgac gattccccgg tttaatctgc atcagtccat gaacatggat acttccattt
    48301 tcatagcgcg taacaggctg ctgaccttct ttcgaacatg tatttagaga ctcgatccaa
    48361 tcttttgata tggtatgtcc aagagtattt tctgattgtt tctctcgttc attccaatag
    48421 tagttcaacg agtggtagat ttgatcttcg ttttgattca atgggcggat ctgctctctc
    48481 atctcaagat tctctgttcg gaaagataag ataccagaag catggtagtc tgtgtttcct
    48541 gctgaagagg tataaagagc cacattatat gaaccgttat taaaacttgt actgctattg
    48601 acgataatat ctgttccgct attggaatcg attcctttcg caagattttc gaaagacaaa
    48661 ctattgatca attcatgagg gcccgttaaa gaagaaccgc ccattttgaa tccattaccg
    48721 ttaccttctc cctcaattcc tggtacccag ccgtttcgat aagcaactga gtttttgatg
    48781 attacttttc cgatcgaacc tgtttcattt ttagcaaaaa ggtcccaacc gtcatctgca
    48841 ttatgatacg cgatacaacc gtcaaataca tttccttctc ctattgtcag tttggccgca
    48901 aatccatcgg catcttcaaa tcctggatcg gcattttcaa aagatgtaca gttcttgact
    48961 aagttatgcg cgggccatgc ttcgaaagga tcatttgagc taccgctgat ttggattcct
    49021 gtatttccat tccgataagc atgaatctct tcgatcaaat tatgatttcc agaaatctgt
    49081 aatccttttt gcatagctgc actatttgta atgtcaaacc ctcgtaaacc ccagtagtga
    49141 ctgaacagtg tcatcccatt tccagttcct ttaaaatcaa gtacaggacg cttgttttct
    49201 ggatttttct ctgcaatcaa ctgaatcggg ttttctcttg tcccgttaac gccttttgga
    49261 atcgtcaatc ctctttcgaa ggaataagtt ccaggcgcca tgacgattgt ctgcccagca
    49321 tatgcgtatc tcaatgcttg tgtcagagat aatggatttt gtctgcttcc gtttcctgca
    49381 tctgaccctt ctggtgttac ataaactgtc gaatatttag gtttacggta gtccactgta
    49441 tgaagaatct cttttgtttc ataactagtc atctctacgt attcttctgg cggatagttt
    49501 ttatctggcg taaaactgaa agtaaattcg ttcgttcctt ccatcagttt tgtctgataa
    49561 tcaaattccc gattcgcagt gatagccaag ttttttgcgt tcttaagcac ctctcctgtt
    49621 gctttatttt taattgtcaa taatccgtca gcatttgctc taaaggacat agtatgatct
    49681 tggtttcctg aatgattact agaggtcaca gtgtaaatcg gatcaatcaa gcgagtaggt
    49741 ctttcttgcc caggaggatc tgtcgcagga tctgagtaag caacttcgat tccttctacc
    49801 ttgatcgtca tattccttgc tgcagcaaat cctgcataaa taaccgattc atctgaagcg
    49861 tataattttt cccagtcata catgatgtca gaagccattt cttctcctgt ttcaggatcg
    49921 taataatatg cctctaaacc agtattcgtt tttttcatct ctacgtccag tttagtaatc
    49981 gagttcgtgg ccggaaacag attcccatga ccgtttccga atatattata agaacctgtt
    50041 tctttttcca agaaccctgc tgaagtctct aaaggtgttg tttccactga gacactccca
    50101 ggagctggcg cagcttgcgg atcttcagat gatattcctg tgattgcccg tgtccccaat
    50161 ccaagtcgca tcgtatattt gctgccgctc gtgttgacca cttcttgtct atccgagtcc
    50221 cagacatatt cgatccgact gcctagtatg gcaaatgaat ttgaataaaa tgcctgccca
    50281 aacaaatgat tttttggtac tgcgtctctt accattaaag caaatccttc ttgggcattc
    50341 gtataggtcc aagattctat ttcgatcgtc gcccgcatcc taaagttgat gtctttgggg
    50401 attgcttgat aataataggt cattccatct gatccgcttg actggaattt tccgccgtta
    50461 ttcgtggaag agagccgcac gccgtcttct aatggttcaa tggcatacct actagcactt
    50521 gtgctttctc cgaaaacaac ccctttccac tcttcatttg cttcttcaga ttgctttttt
    50581 gtcttttcat tttctgattc tttcatcata ggatcatttc cttcttccgt atccatatct
    50641 tcactagttc ttggtatttc ctccattgtt gacatccctt caatgttcac gtgtgcattt
    50701 tcttcgttca cagactgttg ttcgttagca gcaacttcaa cgacagaaat taaaaccggg
    50761 gtactgagca taaggagatt catcattagc gcactgtatt ttatacgttt ttttaacaag
    50821 tgtattcctc ccgtaacaaa ttagaaagcg tttacttaaa gtattaccta atttattttt
    50881 actgtcaata aaaaacaaaa ttataataag tgtattttat aaaaagaaaa aatataactt
    50941 aagttgttga taataaagag atttattaac gtaaaaagaa taagtttttt taaaataacg
    51001 tataaaaaag agcataaaac aaagtcgttt gttttatgct cttcttttat tattttgtgc
    51061 tttttatttt atcaaatcac tttgtttttt tataaaattt ctgcgcctag tgtggactca
    51121 aaatgtctca atgcccattc atgtccaacg ggatcgaaac ttgccacggc atctttgaaa
    51181 atatggagtt tgtatcctag attatacgca tcgactgctg tatgcaacac acagatatct
    51241 gtacatacgc cggtcaaata aatatctgtg atctggcgtt ctcgcaaacg gatgtctaga
    51301 tctgttccac taaaagcgga ataatgccgt ttgtctatcc aataaacatt ttcctcttgc
    51361 tcatagtctt ggtataaact ttttaaagag ccgtacaatt cccgtccacc cgttcctatc
    51421 acattatgcg gaggaaataa gcggttctcc ggatgaaagg aatcttttgg atcatgcgca
    51481 tcaatggcaa aaacgacaaa atccttttga tcaataaact tctttgtatg agcagtcagc
    51541 tttttttcaa ttacttgtcc tgctgtccca gtagtcagct ttccatcctc tgcaacaaaa
    51601 tcatatgtgt agtcaatcga gatcaatgcg ttcatcttag tcctccttat acgagatgtt
    51661 tgacaaattc gatcaccatt tctgctgatc gtttgcctgc gtcttcgata aattcgtcaa
    51721 agctttgtgt cgccgagtga tctgctgtgt cgctcattgc acgtacgatc aaaaatggga
    51781 tattgaactg acgagctgtt tgtccaacag cggctccttc catttcgcaa gctaatgctt
    51841 cagggaaatt cgttaagatt tctttgattt tatctggcga gtcgacaaat gtatcacctg
    51901 taacgatcaa tccttctttt gcgttcaaat tcgttgcttt tgctgcttta accatttcac
    51961 tgcgcaaata tgtgcttgct tcataatata gaggcattcc tggtaattgt cctgggttgt
    52021 agccaaatcc tgttgcatct gcatcaaaat aagctacttt gtctgatata acgatgtcac
    52081 ctacttgtaa gccttcaccg attccgccag ctgaacctgt attgatcacc atgttcacgc
    52141 cgtattgttg gatcaacaga cttgtggtaa tagatgctaa gacttttcct ataccggaac
    52201 gtacgacgat cacttcgtgg ttacctaatg aacctgaaat aaataaagcg cctgcgcgtt
    52261 cccatgataa tggttcgctt aggttctctc tcaaaatttt tacttcttct tccattgcgc
    52321 cgataatacc gattttcatc tttttaattc cttctttcta tataaatgcg attgctaaaa
    52381 aaaccacaca taacattagg ataacaataa taagagattt catcaaaaaa gagttccatt
    52441 tggtcgtctt ttcccgttcg ctgatccttg ttttcgtgat tggtttattc tttttgcttt
    52501 ctttacgata aaagctggca atcttttttt cttcttgctg ataagctgtt tctgcttttc
    52561 gctgtttttt taatgactcg cttgcttgtg cttgttgtcg cttgcgaagt tcgcttcgcg
    52621 taataagcgg tcctttggcc ataagccgat ccctctactt tcctttggtc aatagttccc
    52681 agtattgaat cgcataaatc gtctttgcat cacaaatcaa ctgatctttc atcgcttgtt
    52741 gcgcctcttc aagagttaga tgatacaact ccagtacttc gtcttcatct tgtgctaaag
    52801 gattctcgac cttctctacc ccttgtgcat gataaatatg aaggacttca tttgcaaaac
    52861 ctggtgaaag atacatcgaa gtaagatggc tcagcgactt cgcacgatag ccagtttctt
    52921 cttctaattc tctagctgct gtcatttcgg gattttgtcc ttctcctgga tcgatttttc
    52981 cagctggaat ctccaaaatc actttttcta aaggttttcg aaattgtttg acaagaagca
    53041 gacgatcttg ttcgtcaaaa gcaatgatcc cgacaccgcc aggatgaaat accagttctc
    53101 tcttcgaagt tcctccatct ggcaaacgaa cttcgtctac agcaacatca ataatttttc
    53161 cgctatagat ttctttgcga ctgattgttt tttcttcaaa ttgctcattg ttcagcatat
    53221 cttctcctgc tttctttgta atcataactg cttttttttc atttttcaat ttttctcaaa
    53281 aactccttat tagtttacca tagataaacc ttagattttc tctaaatata gaaaatcctt
    53341 ctttagtccg atactcttta tagtagtccg tatgctaaaa taagtatgta atcgaataac
    53401 gaaaagaatt caatactcaa cgataaatga catggagggt ttattatgat aaaacgaaat
    53461 tggccgtggc ttctagcatt ggtcttgcta tttctctttg tccgaaacaa tagtttttct
    53521 tttgttctag gacttttatt gataggtgta gttttagttt tgtggggact ctttggttcg
    53581 cgcaggaaaa agggaaaaca agaagaaatt cctgcactga ctgacgaact tgaaactcac
    53641 tatgaaaaaa gcggtatgac agcgaatgaa atttcatttt ttcgacaaac aatgaatcaa
    53701 acaaaaaatg agatcaatca attgcaaatg aatatgcagc agacagccaa acttaaagca
    53761 atcgatctcc gccatgatcc agtcaaagca gcgaaagctt tattcaaaga attggtgaaa
    53821 gaaccaaacc gactgcatga agcaagtcag tttctttata cgcatctgcc gaatctagtt
    53881 gatttgacaa ataagtataa tgagatcaat gaccatgaga tcaaaaacaa acagacctat
    53941 gaaaaactag aagaaagcgc gcagatcatc gaccagctgt cagctttaat cgcacaagat
    54001 tatcaaaaat tcgttgctga ggatctagat gatatcgacg tagagatatc tgttgcgaaa
    54061 cagagcttaa agcgtgataa taaatatgaa ccagaacaaa aggattaact cttttgttca
    54121 taaaagaaga ctcggaacat tcagatgatg tctggctgtt tcgagtactt tttctaaagt
    54181 agagacattc atgtaagaat cgagaacaga ttcaacaagg aggaattttt tatggatcat
    54241 aaaccgactg aaacaaaaga tgttacacca gttaacgata cacttgaaga tttattgaac
    54301 aacccgtttt caactccaac agatacgctg accacttcac aacaagcgga gattgatcag
    54361 ctccaagagc aacagacagc tgctcgttta attgacaaac taccggcgga acgccaagaa
    54421 caagctaaac agctagcagc aaaaatcgat gctaatgatg cacagtctgt catcagctac
    54481 ggatcggctg cccaagcaaa attaagtgaa ttctcacaat cgatgttgaa tcatgtgcaa
    54541 gcacaagata ttggtcctgt aggagattcc ttaactgaac tgatgtatcg tctccaagaa
    54601 gccaatcctg acgaactgcg tgccggagaa gggaacatct tccagcgtgt cttcgggaag
    54661 gtcaaacaat cgatctatga agtgacagca aaatatcaaa agattggtgc acaaattgac
    54721 aagatttcag tgaaattaga taaagaaaaa gacggtctgc taaaagataa tctgatgtta
    54781 gaacagcttt accagaaaaa caaagactac tttgatgcac tgaatattta tatcgctgct
    54841 ggagaattga aaatggaaga acttcaaaca acgatcattc cagaagcgat gaaaagagca
    54901 gaagaaactg gcgatcagat ggatgttcag attgccaatg attacacgca attcctagat
    54961 cgtctggata aacgtacgca tgatcttcgc ttagctagac agatcacgat ccagcaagca
    55021 ccacaaatcc gtttgatcca aaacacaaat caggccttgg ctgaaaagat ccaagcatct
    55081 atcgctacag ccattccgct gtggaaaaac caagtcgtga tcgcattgac cttgcttcgt
    55141 caaaaagatg cagtaactgc tcaacgccaa gtctctgaaa caacaaacga cttattgaag
    55201 aaaaattcag aaatgctgaa aatctcagcc atcgaaacag cgaaagaaaa tgaacgtgga
    55261 atcgtggata tcgagaccct tcaaaccact caaaatgatt tgatcgaaac aatccaagag
    55321 acattgcgta tccaaaaaga aggaaaagaa aaacgtcgtc atgcagaagt agagcttgga
    55381 cacatggaag aagatctgaa acaaaaactg ttggatttaa cccaatagat cacctttcgc
    55441 ttgttactaa aacaaacgcc caacaacagt ttattacaac tgttgatggg cgtttttctt
    55501 atctttcttc taacttctcc attggagtct atttctcatt catcttgctg tgtccatagc
    55561 catagccaaa ataaatgatc agtccaataa ccattacgat accgaacacg atccatgtca
    55621 cagtttgcag acgaagcatc aatacaatac aagtcaagat ggagacgatt ggcaatagcg
    55681 gaacgaatgg cactttgaat tctccagctt ttggtttgcc aaacattttt ctcaacttca
    55741 gtaagcctag actaacaagc atcaaatagg cgagcgccat gatattcgtt agttccgcta
    55801 atacattcaa tgggaaaaat ccggcaaaga atacagctgc cacaccagcg gcataagtcg
    55861 cttttttcgg tgtacgatgc ttttgatcga tttctgtcat aaacttagga agtaagccat
    55921 ccttgctgac agcaaacagt aaacgagcaa gtgaatacat catcgagatc gttaccgtca
    55981 gtagcgttaa aattgcccct acagcgatca ccgcgcccac tgttccatgt cctacatacc
    56041 tcattgcaaa agccactggg tcattgactc ctagttcagt atacggaacg attcctgtca
    56101 agatcagcgt cacgatgatg taaaggacag tagcaatcag tatcgaacca atgattcctc
    56161 ttggcacatc tttttgcggg tttttgactt cctcggctgc cattgcaacg gcatcaaatc
    56221 ccaagaaagc aaaaaacaca agagctgcac catctagcac accggaaaaa ccaaaaggca
    56281 tgaatggctg ccagttatca ggtttaacga aaaatattcc tacaatgata aacaacgcga
    56341 tgataccaaa tttgacatat accatggcat tattcaaacg taatgctttc ttggcttcaa
    56401 gcgacacgat gtaagtgacg aatatcacta cgagcaccgc aatcaagtca atgtatgtac
    56461 cattttccgg attgtacccg ccagttaatg cttgtggaag ttcggcccct aaacttttca
    56521 aaaaaccttg catatagcca gaccatccgg aagcaacaga ggaaaccgct agtaaaaatt
    56581 ccccgatcaa caaccaccct gtcagccagg cagcaaattc accaaataca acatacagat
    56641 aggcatatgg cccaccaatc actggcactc gcgaagcaaa ttctgcgaaa cttagtccag
    56701 acaaaatgac aacgattgca gctaagacaa atgaaagtga cagtgctgga ccagcatttt
    56761 gattggccgc aacccctgtc accacaaata ttcccgtacc gatgatcgca ccaattccaa
    56821 gcatgatcaa atcagacgtt tttagttctt ttttcatctc actcgtatga ttgagattga
    56881 tctctttctt acgaaacaaa tccattgaag ttcctcccca ttttaggttg gaaaacagcg
    56941 attaaactgt tttcatatac cctaaaagga taccacctaa aatgactaaa aggcaaccaa
    57001 caataacata aatgagctct tttttcgttt ttctttcgcc aaggatgaaa atcccaccta
    57061 aagtagagat aatgatcccc atttgtgaaa gtgagaagcc aacagctaaa ccaagtgatt
    57121 tgactgttag aagcatgcag atgtttccta agccccataa taatccacag atcatgtttt
    57181 tccacacaaa ctgatcgact ttaactttac ggaaagcaaa gaagcttgcg cctaagatca
    57241 ttccgatact ttgaggtaag ataattgaca tcgcatccaa attcgcccag ttgacgatga
    57301 tggtatatac accataacca atcgtagaga agatcaatgc tctaaaaccc ttaccaaaat
    57361 caagcatttt attatctgta tctatttttc cttcgtcatc ttgtcgggca gtcaaatacg
    57421 caccgcttac aagaagagct aaagcaataa taccgtatac ataatctttc gtttgtgtcc
    57481 attcatggaa gaaaacggct cccgcaatcg tattcaaaat taattgaaaa ccagtagaca
    57541 atggtagccc tacagaaaca cccatgtact tcattccatg gaactgtcca ttttgtccaa
    57601 cactccaagc taagccggat aaaaatccga tcaagaaaat ccaaggggtg attacaggac
    57661 taacaacgaa aaagacaact aaagaaaaca gaaatgcacc gatcgtcatt cctagtgttt
    57721 gttgattggc atcgccacca attttaccac ttactaaacc aatactaccc catgccaaca
    57781 tagggactaa tgctattaaa atttccataa ccaatctcct tttatttcac atgataagaa
    57841 acttatcagc gaaaacgtta acaacacaag cacttttttt cgtttctaac gattttctaa
    57901 cgtttttcat tataaaaatt taagattggg aaaaataaag tcattgttgt cccttttcct
    57961 tcagttgatt cagccgtcaa atggatacct aatttttcgg ccagattttt tgctaaataa
    58021 agtcctaatc ctgttgaatg tgttttgcta agtcgaccat ttctcccagt aaatccctta
    58081 tcaaagatcc gtttcaagtc ttccgctggg ataccgatcc ctgaatcttt aactgaaagg
    58141 ctgactcctt gcgcttcctt ttctatgctc acaatgatgt ttccgtgatc cggcgtatat
    58201 ttgatggcat tactaagcaa ttggttgaag ataaaagcga tccatttagc atcggtcaaa
    58261 acagtatgat cctcaccaac gatctcgtag cgaatatttt tttggataaa ataatttgcc
    58321 tgactgcgta tcacaggctg aatgattcct tttagtccat attcttggat gaggtagtcc
    58381 cgcgagaaac tgtcgagtct tgcataatac aaaacttgtt ctacgtattc atcgattttt
    58441 gataattcat tttctagtaa tgtatacttt tgatcgtcaa tatcgaactc gatcgaacga
    58501 actagcaatt tagaagcgga aagtggcact ttgatttcat ggacccatga gtcaatataa
    58561 tctttttggt cttgttgatt actgattgct gattgcatta cttcttggtg ttcacggatc
    58621 agctgattga tatattcttc taccagtttt tcttcttctg tcctcccacc actcaaataa
    58681 ttttgcaaag gggaagtttt ttgtatgtcg gctaattttc cccaccatct tctcttgctc
    58741 aagtaaccac ccagcagata aaaaatcaga aagacgccct caatcaaagc taaataagca
    58801 attgtgctca aatcaacttt gatgtttggc gccagccaca tcacaaagat cgtcaatgca
    58861 ataaaaaaca gccatcccaa gatcaatgtc cactgatctt tcaaatattt aaaaaaattc
    58921 atcttaaccg ctcatccttt cagggaacga tatatccctg tcctactttt gtttcgatat
    58981 atccttggat acctgcctgt tcgatttttc ggcgtaaccg attgatgttg actgtcaatg
    59041 tattgtcatc tacaaatcgt tcgtcatccc acagtgctcg cagcaatttt tcccgactta
    59101 agatctttcc attgtgcttc atcaaaataa ataatagacg atattcattt ttgctcaaat
    59161 caatgatctc tccgttgatt tccatacttc cgttgtcaac attcaacgta atgccattgt
    59221 ggctcatcat ttcactagat aattctgtgt agttgtaaga acggcgaagt aatgcattga
    59281 tttttgcaat cagtacatcg atttggaaat gttttgtcac aaaatcatct gcccccatgt
    59341 tcatagccat gatcatatcc atattcgtat tcctgctgga gataaaaatg atcggtactt
    59401 tcgatacttc acgtattttt tgacaccaat aatagccatc caaaacggga agattaatat
    59461 ctagtaatac aagctgtggt tcttctcttt caaagtctga gaacacttga ttgaaatcgg
    59521 tcgttccaaa ggtttcaaat tgccatttct caagttcctc catgatcaat tgtcgaatga
    59581 tctcttcgtc ttcgactacc atgatttttg ccataaaaaa tccctccatc tttcttcttg
    59641 ctactacaat atcaaagaag cccttgtgaa acaagttggc ttacctggaa aatgtcattt
    59701 tagcaaacgt taaaaaaaac tggaaatatg cgatttaatc gcgtttgtct cttccaagca
    59761 gcttaaattt attatgttat tcttaataat tggtctatta tctgttattt ttctttcgtt
    59821 gtttttgtct tttaaatttt cgataacacc aataaccgaa aagcgttagc actagcaata
    59881 tacctagaag gagtataagt aatcgataaa gatggtattt ttttgtctgt tctatctttg
    59941 aagagacttc ttctgtaaag ggaactcgtc ttcctgtcac tagtaatcgg tgagtgttca
    60001 cggtgtaagg tgtacaagtg ataagtgtga ctagatcctg cttttctcta attgctaatg
    60061 tatttgtttt tgttggaagg acaacaattt tctcaacgac ttcataagcg agcatccgac
    60121 catacacttc aagatagaat cggtcatctt gctccatttc ttcaagatcc gtaaagagtt
    60181 ttttctcagc aataccgcta tgggcggtaa tcacggaatg tgtactctct cctccagttg
    60241 gaaaagaggt gccttgcagg actgtcgctc ctctatctaa caaaagatca ttcgtttcat
    60301 caaataaagg caagctaaca ttgattttcg gaatatagat ggctccgatc atatgttgtt
    60361 cgtaatattc ttttcctgga ttttggacat ttctaaccgc ttgctcaaaa ggatctttga
    60421 cttgtcccat tcctgggatt actgatgtat gagcaagcgt ctgatttttc ttttcatgca
    60481 ctgctaattg ttgctcatgt ttttcttctt tatcgcgttc aagctgcttt tgataattct
    60541 cgatccttct ttgatccaaa taattgctga gagcatctgc taaaaatgga tatgtaaaaa
    60601 ctgcagctcc ggcaagaaac atcactgcca taaataattg taataaaaga ctgatttggg
    60661 tttgaggtct attacctatt ttctttttca ttttcttcac cttcttattt aaaaaaactg
    60721 cagcaacctt tataaaaccg attgctgcag cttatttttg ttgcttatac ttcagacttc
    60781 attttagatt tacggtacca gctgtacgct ccaatcatca aacttattcc gattagtaag
    60841 aatgccaaga gcccgttccc accagtagca ggaagtgacc cgcgtttcat attcggtact
    60901 ctttctggcc ctggattttc tactcctacg atctgaattt cttcattata gcttccataa
    60961 gagatgataa attcagtaaa ggctgcctca cctgttggaa gtacataacc ttctggcgcc
    61021 atggtctctt tcatttgata ggttccataa tctaaccctt gaacctcaaa gtatccattt
    61081 ccatctgcga caaattctgt tgcttgtccg cttgttgccc actcaattct atctgctcct
    61141 gattcataaa cagctaaagg cgactcccct ttataaaata ttgcgtattc agaacggtct
    61201 tcattccaca attcgaatct tgcatcttta agcaattcac tgcttgatgc atcatatttt
    61261 tcaaactttc ttccacctgt tgtgactttc tctggcggct ctactccttc ttcataatca
    61321 aattcatcat ttcgtccgat ttcaataacc gctgtgttgt tatggaaatc atccgcttct
    61381 agttctgtgg aaaccttcaa attatacgtg atcgttaaat ctcctccacg tgcatctgct
    61441 aatctaccta aagtatctac tttggctact ccattttctt ctgtcaaaat cgtcacgaca
    61501 aacccgttgc ttactacttc tactatataa tcttgtcctt ttgttaaaat cgtctcgccg
    61561 gctcgcactt ctatttcctg atcataataa gttagaccaa ctgtaggcgt atctgtgatt
    61621 ttgtaatttt tgataacagc gcttccgttt tcaaggacag aatcgatgaa ataaggaata
    61681 ggtgctgtga tcgtgtaaga agccgttttt cctctttcta gatcaatata agaaatcgtt
    61741 tcttgttctc cagcttcatt aataatcgtt acttgtctaa ggtcgctttc ctctacattc
    61801 atctgtttat ctgcttctct gatttcgttt ttcggataca aatgcacatc tgtattgtat
    61861 gtgtctttcc atgtagaacc atcccacacg ccatccacca tatcaggcat catcactggc
    61921 atcataacaa ccattggaga cgcgattctg gaaatatgag ctggcgaata agtttctaag
    61981 aaaagataga ctttatctct agaagaaggt gcttcttccg tcacttggac ggtagagaat
    62041 accgcttctc cagcttcatt cgttgtttgt ttgtcaatac ttgtcagtcg atattttaaa
    62101 aaccaacttt ctgtgtgccg tttttgaatc cattcaatcg cttcggcaga attaagaccg
    62161 tcttctcgtt ccggtgttaa aggattatct cgaatcaaat cgtaatatac ttccgttacg
    62221 tcaaacaaat caaaatcgac tcctggaata ttttcttcac caaaatccgt agaaaccaat
    62281 ccgctattcg gtcgttcttc cggaacagat gagaaacctt ttttgtgtaa tgtaatcgtc
    62341 acttcacttg gtttttgtgc gacttcttcg gctttgacta gttttccgtt cccgaaaatc
    62401 cctattcccg cagtaacgag taacagtaca ctcattcccg tgacaatcat ttttttattc
    62461 atttttttct ccccgttctt tttttattaa taaaacagca cctaacaata aaagaattcc
    62521 tagtaaagaa atcaacgttc ttgcttcatt cgtttgcggg aatacggacc attttcctga
    62581 ttgacctgga ttgcttggac cactcggctc tttcggtttg tccgttgttt cttttacggt
    62641 ctcgttataa acacgtggtg ttgcggattc ataggcagag actggaactt tcccggattc
    62701 taattcagcc atctcctgtc cgttaactac tacatattgt ggttggcctt ttgcatccac
    62761 ccaaaattca gggatgatga cctcgattct cttacttgct tcatccatct cgtaaccttc
    62821 cacacctttt aattcttcaa aataataggt gccagaaggc aagaaacgtt gtcctgtcgt
    62881 aacaaggccc tctttatccg aaatgaattt gcttacattt ttgtcattca aaggatcatt
    62941 gtctgctggt ttgatccatt gatttttcaa gtcattagca ggagccatat ctaaataata
    63001 tttcccccct tctatcatct gatatagagc aaaaacagca ccttctaatg gttttccttt
    63061 ttcagttgtt cctctttgac gtccatactt gaagaagtat ggatctctta gataaccgac
    63121 atttttagga tagatatgaa tctcttgcag ttcctcggtt gattctgtcg ggtgataaat
    63181 tggcaaaata gcagcaattg ggatagctgt ctgatcgata tcaacatcca atccaacttc
    63241 tggatcaatc gattcttcga aaatcaaata aactgcatcc cgatcttggt ttttcttagg
    63301 tgctttgata cgtgcaatcc cgtctcctaa tccgccaacc gtatctttcc cagttgttac
    63361 cgaagtccct atttggacaa gcctttcttc tgtgattagc tgacggatat tgtttctgtt
    63421 cgtttcagcg atttgctgga cgaattcttt aggagtcatg ttttcagcag cttttttttc
    63481 atgataataa tcggtcatgt cgtaaatggc aaatgtcgct ccatttagtg gcaccgtctg
    63541 tgaaatgata tctgctgttt cccctgcttc ctcagagata gccaagccat cattttcatg
    63601 gtattcaaat tgatcgttat aatcgatatc tcgaatcatc cgtttatgca acacgaattc
    63661 cacgacatct tcccctgatt cttctgccga catcgcatgt ggaagaagga ctgctgcaag
    63721 cggtagtaaa acaagtaatt gaagcatcaa acacaacagc tttttcatta ccttgctccc
    63781 tcctttttag attgtctgac aaacaaacca taggctgctc ctgcaagcgc catgaagaat
    63841 gcagcaagca ggatatagaa taatcgtccg gatcctcctg tagcaggcaa cggattttca
    63901 ggatcgttcc cgacactaag ctgtaatctg attcgatttg tctccaaaag ttcatattct
    63961 aactcttgct gtagatcagg cgtctctgct tcttcgtttg gataatacga aatctcaact
    64021 tttagtgtac ccgcttcttt tcctggaact acccgatcat tttctatgtc aggttcttct
    64081 cgataactaa tttctaaaat aaagtaacct ggaagttttc tatagctgtc aggtgacttc
    64141 acttccttga tccgatagac cccttccaac aagtctgaag cattgaatag ggaagaatcg
    64201 tttggatctg gtgccaagcc tccctcgatg atttcatact ctcctgcact attttgtttc
    64261 tcaattgaaa attctgcgcc ttcaagacgt cggctattca tactgtcaat tttcaaaagt
    64321 tccaaatcga ttggcgctag ttcatcttct aaggttactt gtagtacacc atcgatgatt
    64381 tcatagcctt ccggcaaggg agcagtttcg tcttcgatga ccgtatcttc aatgataaac
    64441 ttaccttctt cattcagtgc aaactggaac acttgatcag atagccgata cccatttggt
    64501 gcgacagatt ccgagacctc atattcacca atagacagat gcagaggttg gtcgtaattt
    64561 aaatcgacat acttttctac tcctacggca cgagaataga agtatccgat cccaggcaaa
    64621 cgtttcgatt cttctggttc taacggatta ccagtgtatg ctcgatacag cgggaaggaa
    64681 gaatccccca ctttcctgat atcgaaacct gcaccatcta atgtatggcc ttcatggtct
    64741 aattttctaa ctcggatctc ccgataatca aacgtattgt aagcagtgaa tgctacccat
    64801 aaacgatcct cttcatctgt tgtatacgtg agatattgcc aattttcttc acctgtttgc
    64861 ttgagttgga tagtccctat agcattatcg taaagtcgca agatccatgt gtcattttca
    64921 tcaggttctt tgaaaccttc tgggttctgg ctctcaatga ttttgtattc tgcatagttg
    64981 gtagtttctt cactatcctt ataaacaaat gaatctggct ctccgattgg atgaataact
    65041 ttctcaccat ccacgattac atatttcccg tcttcatcta ctgaatagaa cgtgagtctc
    65101 cctttctctt cctctacttc gttcacacca gaaagccctt tcgctactgg atatccgatt
    65161 tccagtccgt taccatctgt ttcataaagt tcaaactctg cgccattgat tggcatatta
    65221 ctgccttcaa ttagtttatt gatattcatg tggattggct tgaattcatt tcgcatttcc
    65281 agtttgacat acaactcatc atactctgtg ccttctatgt taggctcttg tacaacagaa
    65341 agcttgtgat attctccttc ataataggaa ttcggatctt taccttccgg tccgccgact
    65401 ctcgaaccag atgtatattc atctacaagc tcgtagtgac ctaacatctc atcttcgttg
    65461 gctgcatatt ttttgatttg gaattgatac gtctcatcta ctaactgata acgtggtggt
    65521 ccagcgatct ctctgatcca aaatatgtcg ctgactgctc cagtttcatc tcctaaaatc
    65581 ggcatcttcg tttcaaagat cgctattccg tccgtggtag ttttacttgt ttcgattggt
    65641 tcggcttccc cactctctgg attttggtca tataatgcaa attcgactcc agccaacggc
    65701 tcgccatttt gacccttctt gctgacttgg aaattacggc ctttatgttc gtaacgaatg
    65761 gaagaagatg ggaaatacat ccgtccagta gtgatatcat tctcataatc ctccaggttc
    65821 tgaatatagg ccatattgtt cactgtgcaa aaatttccgt cttgagctcc cgcatcaagt
    65881 tttacttggt acttgatcct gaccatttcc ccttcaccta aagaaatcgg agaaaattta
    65941 taagcttggt tttcccgtcc gacatacgtt gtcggagtcg ccactacttc tggtggggct
    66001 tgtccatgac gttcttcatc ccatatttct ggctcttggt acttcatgcc gttttctcct
    66061 ggatctagat ggtaaaactg aatatctgcc gatctgccag ctccaaatgg tgagacatta
    66121 aaaccagttt catcataaag tgtggcttgc tggatcgttc tcttgaaggt agaagagata
    66181 tcctccaagg ctgtaacaat gtcattcttg ttgttcgcat tgtaatatcg cggtgttacg
    66241 ccatcttctt ctaaatcagc aatatttttc aatacatttc gtccaatagc atctagtcgt
    66301 tgtctcccgc taacgtttcc atcgattcct aaaccaatgg agaagatact tggcgggttc
    66361 tcttgatact tgtttttcag ccaatgatga taagcaactg tgttgatcgc agatgacttt
    66421 tcttgcgaag taggactgcc attcgtattg ttattagaag aaccatcacc aacccagtga
    66481 gtcgctttat tatctccttc ttttacctct ccataacgat agtgtacatt tacttcatct
    66541 tctggacggt taaagtcaga tgcataggta actggatgag taaaccctgc attagaagta
    66601 ttcccagaaa actcctcaaa atttctgaac aaaacgtcat tatcttcagc aatcttatct
    66661 tccatcacag tccagttgct ccaagctcca ttagtgctcg tacgataact tgactgaatc
    66721 ggtgcataac tgaaggtcgg tgtcccatct cccacaacga ttaggatttt ttctggattt
    66781 ctattttctc cgccattgtc tttatacaat gtttcatagc catttttaag ccctagtgta
    66841 agcggagttc ctccgattgg tgtacgattt aatgctgtat tctcacttcc cgtcaaacga
    66901 ttgatatcat ttgtcaatgg aaagatgtcc gaactgattt gctcatggtt attgatagct
    66961 gtactattat ggtaaaaatt caccataccg attcgaatat ccagactcgg gtcagagagc
    67021 agatctttac tcatttcgtt tactgcctca atcaacgcag catttttagt ttgactattg
    67081 gtcccagcgg ttaattcact catactcgct gacttatcta gaacaaaaac gatatcgatt
    67141 ggactgattt cctttttctc gcccccgatg acatctagat agacattgta aagggttgtt
    67201 gcatcttcag gatgtagatc agatgctgtg ctgcttggca gaacaggttc tacccatttt
    67261 ttgatgtacg ccccgttata atccaagtaa ctgttttcaa aattccgttc atttccccaa
    67321 agctgaacgg tttgagctgg aacgtttcta tcttcttcgc ctgcagttga tccattatta
    67381 gtagcatagt cgtaatttcg ccagttcgct cccccgtttg tataatcact atcatcgatc
    67441 aagttctctc cgtcatatgg cgagcctttg tctccttgat agcgttcatt gctttgtgtc
    67501 caatttccta acacttttgt atagtcattc gttgtaaact tcatctattc tttcatcaac
    67561 caccacttga agaggtagtt acccttcgtt cacatcgggg atcttttctg tttcaaaaat
    67621 caatgtgccg gtttctgctt cttttgaata aacttttccg acataccatt cttgaccatc
    67681 gatcgtctcg acttttaggt cttctccatc ttcatcatag gaggatagac ctgttccacg
    67741 aacgtttttt acggtaccaa tcccggcggc tgccgcatcg attgctaaac gtattctacc
    67801 ttcattggca ctttcctttt tttcaaaagc cagatgccat tcaagggttt cttctttttc
    67861 aatatacttc ccttcaattt ttgcatagtc ctcatctaag aatacctcat cctcctttgt
    67921 ttctgttgac tcagcagcta catccagggt cgtcatatga tagactgact ggaaatatgg
    67981 tccaaatagc aaactgacag ttactaactg caaaagcttc aattttaacc ctttcacatc
    68041 cttatactcc tttcaaaaat atttgtcttc tttcacaaca atcaacaaaa caatgcccat
    68101 ctaattataa tttaatttaa ttttaatttt aaatcaaatg atatcctttt tagttaaaaa
    68161 acaccaatta tacaacattt attattaaaa ttaaattata aaattttata acgttctcat
    68221 ttaacaaaac ttaagttatt aagacaaatg aataaacaac tacgctttcc tcgaaacaaa
    68281 aaaataaagc acaaatgcgc atttacgcac tcatgcttta ttcgtacaat taattcagat
    68341 aacggcagcg cataggatgc caataacact tcctaaaata gctcctccta aaatgtcagt
    68401 aggataatgt aaaagtaaaa aaatcctcga ataagcgatc aaacaagcaa ataaaaaggc
    68461 taatattcct gcaactggac ttaattcgaa caacgcagct actgataaaa aggctagcgc
    68521 tgtatgtcct gaaggaaaag aagaagtctt cggtttactt gtgtaaagga caagccgacg
    68581 atttttttca taaggtcttt tccgtcttgt cgtttttttg ataaaaagca aaccaacaaa
    68641 aaaatagatc aaactgccga taagaatggc tagaccatat tctctagtat caggtatcaa
    68701 gactaaaaca gcagcaagga gcaaccaaat aaaaccacca ttagagatat tactgataaa
    68761 tttcaaaaaa ttgatttttc gactatccgt tttccgttgc ttggcgaact tgttgataat
    68821 actatcatcg attttagcaa tcaaacgcaa catgttattc ctccttttta agaacctttt
    68881 cttgcgtacg atggataaaa agcaggactt gaaaataaaa caagaaactg aaacctaata
    68941 aagcaaaata gattggtcct gcaaaccaga atgcgacaag aaggatcaac gtgcctgcca
    69001 aatagaaaag cgcgcttttg aaatcattaa aaaacaagaa aaatgcagta atccaagaag
    69061 cttctttttg ttccagttga agcaagattt tgtaactaaa gagaagcgcg cttaatagaa
    69121 gcaaaaacaa cgtagtatac atcaatgtat agccaattcc cccaccccaa tagcggaaaa
    69181 tctgataatc gatcaataga agaagaaaat aaacacttgc taaactatct tgctttccat
    69241 aaaaagagaa gttctcacga aactttttcc agaatagtga accgagcttt ttctcttcat
    69301 tttttttgta atgataaatc atatacagaa ctgttcgact tgcagttcct aatgtgaaaa
    69361 caggcaagac gcacaggaac catagtagct gtaaatggat aagacgtgga atccattcaa
    69421 tgatttctaa gaaataatca ttggcttttt taccaatcga tcgattcatc atccttttga
    69481 acctcctgtc aaatccatcc cttcaatgaa ataacgttgt gcaaaaaaga acagcaaaat
    69541 cgtcgggatc aagacaagtg ttgctcctgc catcagataa ttccattgtg tcgttgcttg
    69601 gttctgaaaa acttgcaagc caatctgtaa cgtgtatttt tcttgatctt ggatatataa
    69661 cagaggacct aagaaatcat tccaagctgc gttgaaggaa ttgatcgcaa tggtaatcaa
    69721 ggcaggtttc gtcaatggaa gcatcaaatg actccaaata tacaaatgat tcgctccgtc
    69781 gatttcagca gcttcgatca attcattatt gatcgataag taaaattgac gcatcagaaa
    69841 tatattgaac gcattgccga agaacgatgg aacgatcagg ggcaaataag tacctaccca
    69901 gccgatcttg gagaataata catattgagg gatcatcgtt acaaaccccg ggatcatcat
    69961 cgtagaaaga accaaagcaa acatcagctt tttcccagga aaatcaagct tcgcaaatcc
    70021 ataagcaata aaagaatttg aaaggacatt accgatcact actagcacag tgatgaataa
    70081 tgtatttctt gcatagcgcc aaaaaggaaa ggcggcaatc gtatccagat agttttccca
    70141 tacgattttt tctgggaaaa aggtcggagg ataacgaacg atttcttcca ttggtttcaa
    70201 tgaagtaaac accatccaaa gcaatggaat gatcaaaatg attcctatta ctgtcatgag
    70261 gacataactt agtatttttt ttgtttgccg attcacactg gtctttttga aataatccat
    70321 catttgccgt cacctccaga ataatagacc cacttaggtg caagcttcaa attgacaaac
    70381 gtcagaacca acacgatcag aaacaggatc attgacatag ccatcgcata acccatatca
    70441 aaagctacga acgccttgtt ccacatatgc agattataaa aaagcaaaga atttgcaggt
    70501 ccgcctgttc catttttcgt catgacataa gcttcttgaa aaatttggaa agaaccgatc
    70561 gtagaggtga tcacatcgta aaaaataata ggagtgatta atggcaaagt aatacttttg
    70621 aactttcccc aaacgcctgc tccatcgatt tctgctgatt catataaaga tctaggaaca
    70681 ttttgtaatg tagctaagta aagtagcatt gctcctccag aactccatat tttcatcaaa
    70741 atcaacgcag gtttcgtcca ttgtggatca aacagccaag ctggtccagg gatatggaac
    70801 cagcccagaa atgtgttgat caatcctgca gatggagaaa gcagctgcat ccataagatg
    70861 tagactgcta ctcctgataa gactgccggt aaatagaaag ctgtacggaa tacactgatt
    70921 cctttgattt tttgattgag tactgtggct aaaaacacac tgaaaatcgt cgtcagcggt
    70981 acattgaaga tgacgaaata aacggtgttg tataacgatt tccaaaataa atcatcgtga
    71041 acgaacattc ttttgaaatt tgctagacca atgaaattca tcttggatgt caaattataa
    71101 tcggtgaaac taccaataac tgaaaaaagc ataggcccta gcataaatac caagaacccg
    71161 ataataaaag gcgagataaa taaataggcc catcttgctt cttttttctt tcggatagat
    71221 aaacgcttca aattttcttg cctcctctgt acctagacag tcaaaaatgg attattgatt
    71281 ttgcttgact aagttttcta ctgctttttg tgcatctgcc aatccttctt ttggtgattt
    71341 tgttcctaaa agaatctggt caatctgttc attcactaat gtcgtgtaat ccggagcatc
    71401 taaaggaact ggtgtgatca ccgtattttt aaagttttcg tccgccattt gatagatcat
    71461 ttttccttta tccgttaggt tgtcattatt cactaaattc tcattggctg tcttgctcgc
    71521 catgatatca aaactttttt cgccgaattt ttcttgtact tctggagaag aaagatattt
    71581 gatgaattca taagaggcct caggatcttt tgctccgtat ggtacttcca agacgaatcc
    71641 gccaccccac gaccaatggc ctgaaccttc ttctttttct gggagctgca tcacgccaaa
    71701 atcaaaatca tccggtgcat tttccatcaa actagagtaa taattgatat tttgggcacg
    71761 catggctact aatccggaaa taaatggatc agatacacca gacccaaatt cggcttctaa
    71821 tttattgatc gtatttttcc cataataatc ttgccagtct tggatccact ccaaggcttc
    71881 cactttattt ttagtatcaa tcttgacttt ttcatctttg tcaaaccaac tagtcccgtt
    71941 atctgcgttt agcgcccata catcagcccc taaattccat aatggataaa aaccgatgcg
    72001 tgtaaattct tttccttctt ttttatctag tttacgtgct gtttcttcta attcatccca
    72061 agttgtcggt agatcttgtt cactgattcc agcttcttta aataactttt tattataaaa
    72121 aatgacttgt gtatctgtat tgaatgggat cgcataagga tcaccttcat attctactgt
    72181 atcccaaagc tgcggataaa aatccttaga agtgtctttt tctacatatt tggagagatt
    72241 agttgcttgc tgtgcttctg ctctttgggc aacggaattt atgtcctgta caataacatc
    72301 gggtgcattt ccagctgtta ttgcagataa agatttagtc cagatgtcac cccatggctg
    72361 atacttataa tttacatgga tctcgttttg tgattgattg aaatcctcaa tgatttcttc
    72421 gatagcttct tttcgtgcac cggatcccca aaaggaccag aaatcaatat tttttcttcc
    72481 atcacttgtt ttttcagaag aatttgctgc acttgaactg gcatcgcttg cattacttga
    72541 tccgcatcct cctaataaaa acaatgctga acttgccagc aaccctgtaa acaataattt
    72601 tttcatattt tacctccaaa ttttaatgta accgatgccg gttttaatat aacgatatca
    72661 ttttgttctg aaaataacaa tttataaggt atgattctaa aaaaattatt aatgatacca
    72721 atactttata taattcgcga gcgctttctt tgaatttttt aatctttcgt agcagaatga
    72781 gaatgtatta aaaatagatt aggaggaatt ttgatgttta gacaccagaa agaattgcag
    72841 tttgaagcaa aaccagatcg tccgaatcca ttaatcgcga aatatctgca ggagctgatc
    72901 ggcggccaat atggcgagat gagtgtagct atgcagtacc tgttccaagg ctggagctgc
    72961 cgaggagaag aaaaatataa agatatgctg atggacattg caacagaaga aatcggccac
    73021 gtggaaatgt tggcgacaat ggttgctcgt ttattagagg gtgcgcctgt tgaagatcaa
    73081 gaagcagcga tgcaaaaaga tccaacaatc ggtgccatta tctctggaat gaatccacag
    73141 cacgcgatcg tagctggctt aggacctcgt cctgctgaca gcgtgggcaa tccatggagc
    73201 ggtgcctata tcattgcgag tggcaacttg ttagcagact ttagagctaa tttaaatgcc
    73261 gaatcacaag gaagacttca agtagcacgt atttacgaaa tgatcgatga tcgtggcgta
    73321 aaagatatgc tgtctgttct ccttgcaaga gatagctatc atcaaaatct atgggaatgt
    73381 gcagtgaaag aattagaaga aaaagaaggt tcaatcttag tcccaagtac tttcccgcgt
    73441 gacaaagaac gaaaagacat tgcttatgac ttctataact tctcagaggg agaccaaagt
    73501 agtgaaggtt cttgggctag tggcaaagca ctggatggcg aaggcgaata tcaatggttg
    73561 aaagaaccta agatcgaagg cgctgcacca aaattagaac cagtaacacc aaaaatgtat
    73621 gggacacctc cattcaagaa ataaatatct acccgtagaa acacccgttt tttgttccac
    73681 acgctttgtg agcaaaactg actaaagtca ttaatcataa ccacccttca aaagggtggt
    73741 tttctctaga gctataagct caaatcaccg gccagcgcct aaagacgctg gctttcatat
    73801 tttatgttca agccatattg cctttgccag ttttgcccac ctcttaaaag gtttatgtac
    73861 tactccttag cccttaaaag gatcgtctta ctctcgtaca ctcagcttat ctaacgcact
    73921 atcatgtttt tcttgttctt gaatgtactt tttgatggtc gcttcatttt gtcctacttt
    73981 tgtcacatag tatccttcca cccaaaagtg tcgataccca aatttatatt ttagatttgc
    74041 acaatctctt gatgatctcc tgaatattgg ctcgatattg attataaatt atttttcttc
    74101 tatactttgg tgtgaataca atgtggtatt tacacatcta ctttgtacgg gctaaactat
    74161 ttccctttat tgctccaccc gcatagcaag tggttttgta tttcaatcgc tcggcgcgct
    74221 caattaggtc taaagacaat aaatctaaaa acccgctgct tcactgacag aaacaacggg
    74281 ttttcagaaa aaaatgaaat gaaaaagatc tcttacggca atctatttta tttttttgat
    74341 tttttataaa tggaaaatgc ttcaagaagt ccaagaaata aagccgtata aaaacaagaa
    74401 cctatgaaat ctttatttat tagatattca acagaaatcc cgataacact agccaacaat
    74461 atatttatca gaagtgtaaa ataaggatgc tttttataga atagaactag tgttgttttc
    74521 atagactctc ctttcttcat atttccaatt aatcacaaat tcacggcatt actagaaaaa
    74581 ttaaaggggc gcagaagtgg aaaaatactc ttcgactttc cctacaaacc aggccatctt
    74641 gtgtttaata actataccaa atctaaggta cctctaaatc taaattccat ttgtggcggt
    74701 gacctccaat ttttaactct tctatctccc atccagtata cataaccttg atacttcgac
    74761 cacttatata tcgaacacac aaatacctat ccattgcagg agtactacct aacttagttg
    74821 ctacttgcat ctaaataaca gctgaaaaga taggacttac tcttagtaaa gtatctgaac
    74881 tattaatttt taaactatca tttttatcct caactacatg cacatctgtt gcaacttcac
    74941 tttctgtctc atccagataa atgattaagg aatcgggaat tgcaatatct tcagctgacg
    75001 ctggaatagt tgagctaaat tagtcatcat atctttatta taaaataacc aacagcaaaa
    75061 aatacaatga aaaacaaact tggaactaat gtatcattaa taaatgttga aaaattcagc
    75121 aactcattac tgtatgattt tagcagccca accaacatat accctataaa agctaataag
    75181 atggttagga atcctttaga atttttactc attcatagac acttccttaa ataactttta
    75241 ttatagaagg tattgtaaat aataatattg acaaaaagtg actcaaaata aaaaaaaact
    75301 tacattatac tggaatgaac tgtattgata gtgtacctaa aagctctact gacataagat
    75361 cgtgtaatcg gtaaagtaat tttattaatt atacgggatg taatcaatta gagaataaac
    75421 taatcataaa aggtactttt acattcataa ttgtcgcttt gagattctac tttggttcac
    75481 tatctgaccc aattgcacat tttcgatcat tacaagtatt ggtgtcgtta gaacaactaa
    75541 ataaaaacta gcggtagatt tttcagaatc tatgctaatt tctgtatgga tgttccagtg
    75601 atggcaaatt tctcttttca ttgtttttct tgtcgcatga tatgcctttt tgtgttattc
    75661 agttaaacta tagaaattct ttcctagaca gatcttgatc ccaaatttta cgtttttgag
    75721 catcgatata gtttaaaatg acattactca tatctaaaga gtttagattt tttaggacta
    75781 aaactgtcag ttgttctttt tcttctttct gttaaagtat tgtcgcgact tttcgtatac
    75841 gttaagatat gtcgtgaaaa aacaagtttt gtgacacaaa aaaacgaatg acttgaggaa
    75901 atcacctcaa tcattcgttt aaaatgctat atattcttta gtttaagata cggaaattaa
    75961 tgccatcgac cacactagta gaatgaacga catttacttg gccttttttt aatttcgtta
    76021 tgtttagcat ataaaaatat tatagagttt acttacaatg cttaaaacat atactgtata
    76081 atctgatcaa ttttagccat gtcttgcaat ggtaagtttt ctattttttt gagttttcgt
    76141 tccttaaaat caagagattt taattgtgta ataattactt gtccttttat atctagctct
    76201 tcaggtaatg agtaacgtgt cggtaaattc tttatagtgg aagtaattgg acatatgacc
    76261 gaaaacattg ttttacggtt aaattcataa cgagagacga ctagtcctgg tcgtcttttt
    76321 tgaatttctt ttcctacgga gggatcaaaa tcaatccata caatatcacc ttttttagga
    76381 atataaattc tttcttctga cactataaaa tttctctccc ttcaggagtt aaatcttccc
    76441 attcgtcttt ctcataatac tcggcctctt ccccaccact aaatggatct tcaatttttg
    76501 gaactaacgt aattgttcca tcatcagaat acacaacgat atattcttgg ttttctgaag
    76561 gtttctttcc gttatcagaa ggtaaagtta ccactacaga acttccttgt aatctagatt
    76621 ttgtaacaag cattatcatc actcccttaa taaaagtata ctatataaca ctaagtaacg
    76681 caaaattccc ctttgtaaac tatctagaaa attgttttgt ttaaagtata tcttattgga
    76741 tataaataga aataaattgt agccagttca ttttttcttt aactaaaaaa ataatccaag
    76801 aaacattgaa cctatggatc caaaagctac ccaaatacta tagctaaccc cccgctacct
    76861 tcaattttgc agataaaacg ctttgggaga aaatttcaaa gacaacaggc catcctatca
    76921 tgcccgatta taaacaaaaa gctggctatt ccttttgctc tgacaaattt caattgggaa
    76981 agttcacgaa actagcaggt gaagaagttt caactgttcg gatcaaagaa tgcccgcttc
    77041 aaatagaaac attggttcaa cgaatttcaa ctagacaaga ctttgctatt gtagaacgtg
    77101 agatcgtagc tatttttgta gaagaaggga ttttacatga tccaactcat atcaatgtgg
    77161 ataaatggca acctcttatc tataaattca gagaatatac aactgccaac cagtcgttaa
    77221 ctttgaattt tagattccaa gaattcaaag agtcataatg ataaacatcg ttaggtataa
    77281 ataaaaaaga ctcgcagcac gagtaaaaaa caaatagaag ctatacatta gaaaggtcga
    77341 ctgtgaccct atagatgcag tatatgcagc ttatgagcat ttcttctgtt ctctttgttt
    77401 ttgggatctg tgctgaagtc tttttcgttt tttgccgctc tttctttgaa aaatcgatta
    77461 tgttatacct tctccattac taattcaggc atctgctgag taatgatttt tcgcgcaaaa
    77521 gaaatatctg ttacccaagg ttcgatttgt tcttcagtca agacaagcgg cattcggtca
    77581 tggattggtg cgactgtgcg gttaggttca gtagtcatta tgattgattc tgccattttt
    77641 tcatcctgat ggctacgata aaaaccgcca atatacacta ttttttcctt atttgaaaac
    77701 ataaatttgt ctttttcttc attccactca ttgggaaaac acaacgattt tgcaaaaatg
    77761 gttgatgaaa ggtctttttt tcttctactg tctctgcacg ggcattaatc atcaattgtc
    77821 cttttttaaa tccggtaaat ccccacttca tgatttccgg agtgaggatg ttttcttcct
    77881 cttttcttaa cgtcacaaca agatttgacg gaaaaacttc tccagctgct aattctgctg
    77941 cctgcttcgg ttgtttcgtc tgggcttctc gataataatt ctgaaggagc tctgactcta
    78001 gatcaaaaaa ataacgacca cacatcctaa tccctcccaa tctttttttc tatcatacac
    78061 tgcaacaaga agttcgcacc aacaaaacac tttcaatttt tgtagaacta aaaaagttgt
    78121 gacaagtgac aaactctcca gtgaatggta ttctcctgcc aactcctcgg tcacaagtca
    78181 caatattcaa caaacatgag aagctgcttt ttgaaattgt tccttgtgac tcggagtgaa
    78241 acggcaggct tacgacctct gggtaaacgg tgttcaaaag ccagtttctc ggtattaagc
    78301 caaatttatg caaaaatgta gaaagctatt ttcacaaaat ttttcttaat actcgaaact
    78361 aagcgacttt ttcacaacct cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat
    78421 aagcccaacc aaacgaaaaa tatgagcagc tatttttatt tggctgttct tattacttga
    78481 tgcagggcag ctttttcaca acctctccac taaacgatgt tcaatcagct aaccttcggt
    78541 attagctcaa aaaacggcaa aatatgagga actattttgc cgtttgtttc tctaatactc
    78601 aggtcaaagc gctgatttca caacctctaa tacacggtgt tccagaagta gcttcttcga
    78661 aaaataagcc gaaatttcac aaaaatttga gaagcaattt tcgtaaattc cttcttattt
    78721 cttgaagctg accacttctg tcacaacctc taattattaa agaacctttt gcgaagttaa
    78781 caatctttct ttgaccaatt gaaaattctg cctaagtagt gatagatcct gtgtgacttt
    78841 caacaataag atacttccct ggatttcagc aatcaacgct ttggcttgac ggtctgcttc
    78901 ttcatcggac agtttttctt cagaaagtgc caggatattt tgtttcgtat acttgaccca
    78961 ttgattgact aattctagta gcaacagacg gtattcttca tccactgtac tcaactcaat
    79021 aatcaaatta cctacaggac agccatgata agtgatctgt gtctcatgat cagcgaaaat
    79081 ccagtccaac atttcttcta atgcttcttc tggctgtttt ccagcattta agatgtcttc
    79141 gaacaattct gtctgccata cgtccatatg cgcatggatc accgctagac aaacttcttt
    79201 ttttgaagaa aagtaataat aaaattgacc ttttcctgta tcagaagctg ctagaatatc
    79261 tcggattgac gtttcatgat agcctttttt ataaataaga tctttggtcg cttccaagat
    79321 cttttccttt gttttcaatg atattctcct ttttcatttc tcatagtcct atattataca
    79381 gtaaattcat cattttttct tcttaatcgt ttaaaaaaga ctgtgccaaa ttatctgaca
    79441 cagccctcac tataaaactg ttctcatata gctagatgtt cattgattca agaactgcga
    79501 ggacaaaagc aattcctatc ttatgatagt tgctctaata cttcctctgt tgtcatcact
    79561 tgatctgcaa taaagccaaa gttcgtcaac gctgcatcca ttgcttcttg gccaggagca
    79621 ccaattgcat ctgtcgctac cacaacattg aatccactct ctaccaattc acgcatatgg
    79681 gaatcgctac acaagtttcc gttcattcca ccaagaatta ctgtgtcgat tccatttttt
    79741 ctcaattgga gcgccaaatc attgctttct ggtccaaaga ttttatgagg agagctgatg
    79801 atcgtatttt catccaaata aggtttcaat tcttcgataa aatcggctcc tgaaccttct
    79861 tccactgatt catattgact ttttctagca aacatctgct gctcaagcat catatgttct
    79921 cctgcaccgc caaattgcca tttatggtca tgtgggtaaa aataatgagg agaaataaag
    79981 agtttgtatc ctttttcttt tgcttgtttt accatttcaa tcatattttc gatcgtattg
    80041 tatttttcta agatatcttt tgtcaaacca aagccaccgc ctgctggttt caagaactcg
    80101 acttgcgggt cagtgagaac tactgctgta tttttattat tgaacatggt ggattctcca
    80161 ttcgttattt attttaatac atcatttaga gcttcactca ttgtcggatg agaatagatt
    80221 tgatctctca ataacgtata aggaagtttt ccattcatag ctagcgaaat caagttgatg
    80281 acttcatgag aatcttccgc atagatactt gcccctagaa tcaagtcagt ttcagggtcg
    80341 actaaaactt tgaatacacc tttaggatct tctaatactt gtgctttagg gacaccgctt
    80401 gtcatatact tgaataattt gtaattgacg ccttgtgctt tggcttgttt ttctgttaaa
    80461 cctacattgg ataaagtcgg agtgataaat acactgtaag gcacagcttt ccggttattc
    80521 gttgttcgtt tattctcgcc cttcaactga tccataatga ttcgataatc atctagagag
    80581 atataagtga actgcaatcc gcctttgacg tctccaatcg cccaaatgtt gtctgcatct
    80641 gtacgaaggt attcatccac agcgattgct ccgcgatctg ttagtttaac atctgtattt
    80701 tctaatccaa gtgatgctgt acttggtttt cttccagttg cagccaaaac ttttgacgct
    80761 tggattgtta cttcttggcc atcttctgta aaggtgacgg cagctgttga ttcttggtct
    80821 gctacttgat ctacagatac acctagatgg aattcgattc ccgcatttgt catgtcttct
    80881 aaaatcattt gcgcgatatc ttcatcttca cgtggaagaa attcagaatg ggcatcaaga
    80941 acgacaactt tagatccgta ttcattgaac attgatgcaa attccaagcc aatatatcca
    81001 gccccgatga tcactaattt ttccggtaat tgttcctgat ccatcgcttg cgtagaatca
    81061 atcaagtagc gacttgtatt caatcctttg attggtaaaa tgattggttc agaaccagta
    81121 ttgatgaaaa tccgttcgcc ttcaacaatc actttttcac cattattcgt cacttcgatt
    81181 tgatggttag aaagaaaacg tgctgtaccg tccatcactg tcccagttgc ctcatcgctg
    81241 atcatatgat aatttttggc gcgaagcatg cccgtcaatt tttctttccg agcaacagcc
    81301 tctgtaaagt cgattcctct ttctccattg aaaatcaatg atttagaagg aatacagccg
    81361 atgttgatac atgttccgcc atacatacgt gttgactttt caacgactaa aaccgattca
    81421 ccttttgaag ctaacgcctt cgctaaagtc ttaccgccct ttccaaaacc gatcacaata
    81481 ttttgatatt tttccatcat ttttccctcg cttctttttt acctaccgat tggtacaaaa
    81541 atatgataac cgataattgt ctattctcca aatcatctgc tttatttgtc aaaaaaaacc
    81601 agacaaaaag cggtcctttc acgtttttag tctggttcga tttcctttat tcttctaaac
    81661 aatcgttact tctacatcga tatttcctct agttgctttg gagtaagggc agaattcatg
    81721 cgcatcttct gcaatttttt gggcttcttc ttcgctgatg ccatcgatat gcacttcgaa
    81781 tactacaccg atctgatagc cgccatcatc taaattataa agagagacac gtgcttttac
    81841 tgtggactct gctgtcattc ctgcttcttt gatcatatgt tgtaccgccc cattgaaaca
    81901 agaagcataa cctgctgcaa ataattgttc tggatttgtt ccttctgcat ggattcctgg
    81961 aggagtgatt ttcatatgca tcgagccatt tggtgcctcg acttctccat tgcgcccgcc
    82021 atggttgatc attgctgttt cgtatgcttt tttcattgta ctgctccttt cgattgacca
    82081 tttgctactt ggctataatt atagtgtatg acaagtaagg cgctttctac aaaaattctg
    82141 cttattcgaa taccagaatg ccgtcttcta ctatttttcc aaaaaacaaa aaaatactac
    82201 tcgtgaacaa cagcgtcata tttgttccaa aataaatagc aatcttttct acataatctt
    82261 ttatatgatt gattttaaga tacatatata ccagaaagtt gaccccataa accaatactg
    82321 ttaaaaaaat aaccatattc gttaccattt tttcctcctt ttgctggtct ccctaccagc
    82381 actcgattta accataccac tttttttgcc gtccaagcaa ctagcctttc aaaaagactg
    82441 gtttattctg ctgttaatga ttcaagatca gaaaaatgtc cgaataattt tgctaatttt
    82501 actaacgcca acttgttctt tccgaattca tcattttcta tctgtaatac tagtaacggc
    82561 tcatgtaagc ttaaacggat caaaaaccag ccgtttccgt atggctcttc tagatcatat
    82621 cgaatacctt cttcattttc cgggtcgaag ttcatccctt tttcttcttc caacagtcgc
    82681 ttcatttctt ctaatatctt gtttccttgt tcgcgtggtt catcgctgaa caattttaat
    82741 cggatttctt gtacttctac gggctgtttc aactctttga tttgttcatc taatgtgata
    82801 ccttttcttt ttaattctgg caaaagcatc aggatctttg cgatgacata tgcaccatca
    82861 tccagaaaat agttttcctt gaatgcagcg tgtccgcttg tttcgatggc tagctgtgta
    82921 tctttcccaa gacggttaag ttctaatgct ttattgatta catttcgata gccggaaata
    82981 taacgaactt ggatgcctcc tttttcttca atgaatcttt gtagatgaga agaagtaggg
    83041 gaattcgtca cgattcttgt tcctggatgc tcttttaata cgattgtact taatactgcg
    83101 atcaaattat ttcgattgat cactgttcca gaagcactga caactgcaga acgatccaca
    83161 tctgtatcga aaatgatccc aagatctgct tcatttgcta aaacagcctg cttgatactt
    83221 tccatcgctt ctttattatc aggatttggg atatgattag gaaatcggcc atctggttcc
    83281 aaaaattggg aacctgttga atccgcacct aagctattca atactttttc agcaaagaat
    83341 ccaccagcac catttccggc atcaacaatg attttccaac catttagtgg tttgctttct
    83401 agtgttccaa tccccttttt aatttttgtg actaaatctt gcgcataagg tgtcaaaagg
    83461 tctgcttttt ctacgcttcc agtttgttcg ctttttattt tttgctgctg tgataggata
    83521 aaagcaatat cttctttttc tgctcctcct tctgaggtaa agaattttat tccattataa
    83581 taatagggaa gatggctagc agtcagcata accgcagcat cgcacgagaa ctgttcaaat
    83641 tgagtggaca taaacatagc aggcgtagta gctaattcga aatcgatgac atgtatccct
    83701 tgttctttca gactttggat caacgcctct ttcagtgctg gtccgcttat cctactgtca
    83761 tgaccaatcc caatcgtcca ttttttattt ggatcggttg atttcttctt tttctttatc
    83821 cagtttgcta gtccccagcc gatctttttt accatgtctg tcgtcaagtt tacttcatat
    83881 ccttcaactg gtaatgcgat tcctcgaata tccgatccat tttgcagcgc tttcatatct
    83941 ttcatataaa agtcctcctc atttattccc tcattttttc cagcttttct gcaaatcgct
    84001 gtgcttcttc ttctgagcgg caaataataa tgaatgtatc tacaccggcc atcgttccga
    84061 caacttcagg gtaagccact tcatctagcc aattcgttac cacatcggca ccatttcgtt
    84121 cagtcaatac gataaccatg aactgaacag actggatacg caaaacctcg cgtctaattg
    84181 ctgtacgcaa acgttcttca tttaaagaaa cagatggctg gttaaataat gtatagcgta
    84241 ctattttgtt ttcattttga ctttttacta acttcatttc tcgaatgtct cgagaaatcg
    84301 ttgcttgagt cacatcggcg cctgcttcat gcagccgcgc aagcagctct tcctgtgtcc
    84361 caataggttg ttctgtaatg atctggcgga tcagtgcttg tcgctgtttc tttttcatat
    84421 tagacacctc tcaatgttga ttatagcaaa agataagtaa tatttcttca tattttggta
    84481 ttttttactt ttattttttg ttttcattca ggttgctaac ctttttctat tttaacacgc
    84541 aaaatcgtct tcattttttc aggtgctgat tttcttggat cgctgatgta aatctctttg
    84601 tgttctttag aagctctttc atagccttct tttgcgatga atgcttctaa tttctcaaac
    84661 gttgctggtt cttcggcata gggcccgacg tgtaagattt gggcacaaag accttcttcc
    84721 atctttttga atactacttg ttctttcaag tcttccggaa gtttgctaga acgctgaatg
    84781 gcttcttgtg ctatcgcttc tgtaacaaag tcaggctgtc ggatcatcaa ttgataagaa
    84841 aaatgttctt ttttcatcac tggttcatgc aaatactttt cttgcagtcc ccattgtcct
    84901 tccagtggat agacagtata catctgataa tcaggaatca gccaattttt tttaggcgac
    84961 atgcgtattg catagctgac tgcatataga caggctactc ttcgtttaaa gtcttcctga
    85021 ttcgggtcgc cgatccctct gatgctaaag aatgattttt taggaacctc tagtatttgc
    85081 gggctttttt tcatactata gctcttgtct ttacgccatt caattttttc catttttctt
    85141 cctccgcgaa attccattta gttatttatt atcatacctc tttttgagtg acttggctaa
    85201 tcgtgttgac actatttttc agtttgtgct agaataagtg aagtaaataa atcgtacaga
    85261 taacacgttt cggcgtgtcg agagagaggc ggtttctatt tttataatag aaatcactct
    85321 ctttttttac gaaatttttt agaggtgatg taaatgacaa aacaaaaaca gctgctttta
    85381 gtacctgcag taattgcaac agggatgatg tcttttgcgg gcgtattgat cgagacggcc
    85441 atgaatgtaa ctttcccaac tcttatcaga cagtttgatt taacgactgc acaagtccaa
    85501 tgggtaacaa cgatttatct tttgatgatt tcgatcattg ttccgctgtc tacttatttg
    85561 aatcgcaatt ttagtctcaa aaaactcttt ttagcttcaa atctgctttt cttggcaggt
    85621 ataatgatcg atttcttctc tcctaccttc atggtcctat tgtttggtcg attgctccaa
    85681 ggtgcagcga caggtatcgc tcttccgttg atgttccata ttatcttgac atttgccccc
    85741 ctcaatcgac gaggcgcgat gatgggtgtc ggtactttga caacggccat tgctccagca
    85801 attggcccca cttatggagg cattatgact tcacatttta catggaatca tatcttcctg
    85861 tttcttgtgc ccgttttgat tctttctttg attatcggct ggtttgctat tcccgagatg
    85921 gcagtacaaa aaagtggtta tttggattgg ataagcgttt taggaatttg tcttttgttt
    85981 agtggaagtc taacattttt gagtacgttg ggaacagttt ccagctggat tgctttaatc
    86041 atcggcggta ttggctggtt ccttttttac tatcgaagta aaaaagtgga gcatccactg
    86101 atccgactgt ctattctttc aaataaaaca ttccgcttgt ttttgattgg cttcttagtt
    86161 tatcagtttt tattacttgg tgtatctttt gtcttgccga atttccttca gatcgttcaa
    86221 ggaagtagtc cttttgtcgc tggattagcg atgcttcccg gcgcagcggt tggtgctttg
    86281 ctttctcctt tttccgggaa aatgctggat caatatggtc cgaagaagcc aatttttgct
    86341 gggctgattc tttctttgat tggttggcta gcattattcc tactcatagg acacgcttca
    86401 gtgggtgtat tgattgcttg tcatgtgttt tatatgatcg gtatcggatt atcttacagc
    86461 aatatgatga ccaccggaat gaatgcactg aatgaagaac tacaaggaga cgggaacgca
    86521 atgttcaata ccttgcagca gttttctggc gctgtggcta cttcattagt agcggtcatt
    86581 atcaattacg tccagcatca tacttcccat aactatgaag tgtctactac attaggatca
    86641 aaagcagcat taggcgtctt gttgctgttg cttctcgtaa gctttgctcg attcgcctat
    86701 tatctgttct ttgctaaaaa agcgtagatt ccatatacgg caggtgttgc atctgccgtt
    86761 tttttgtgtg aaaatggttg tttgtaaaag gaggcttttt tatggaaaaa attgccatta
    86821 tcggtggcgg gatcattggt atgaccctag ccagttactt agatacatca aaattcaatg
    86881 ttgctttatt tgatgaaggg acaggacagg caactaaagc tagcgctggg attatctccc
    86941 cttggttgtc gaaacgcaga aacaaacaat ggtatcgtct ggctaaagac ggcgctgctt
    87001 ttttccctaa acttataaaa gatttccaac ttgatcaaac gatttacgca caatcaggga
    87061 cacttttgct tcgacctgaa aaagatttag ctgaattggc tgttcttgcc gagaaacgaa
    87121 aagaagaagc gccagaaatt ggaaacatcg aatggctttc acctgaacag acatctcaag
    87181 cacttccgtt attgaaacca gcagtttcac tgcgtatcag tggtggtggt cgtctggatg
    87241 gaaaagcata tttagaacat cttggtcagc gtttagcaca aaaacaaatg gagcagatcc
    87301 aggcaaaagt cactttgcat aagaagagcg attcttggct catcaaggga ccgtttggag
    87361 aatacacagc tgatcgtgta attttgacac ctggcccggc acttaagcct ttattacaag
    87421 aattaggcta tgctgcagat atacgtccgc aaaaagggca gctcttagca tttcagacat
    87481 cttataagat gagtcaagac tggcctgttg ctatgcttga cggagaagct gatttcattc
    87541 ctttcatgga tggaaagatc cttttaggtg ctacccatga aaacgatcaa ggctgggatt
    87601 tggctcctac tagcgaagct tataagggat tgactaagag tacagaacag ttcttgactg
    87661 atccagatca gataaaaaac tggccatacg agttccgtgt aggtacccgt gcttatacgt
    87721 cagatttttc cccatttttc ggcgtactgc cagatgatcc aagcttactg gctgctagcg
    87781 gtctaggctc ttctggcttg accacaggtc cttatatcgg ctatctatta gccaacttct
    87841 taaatactgg tcattggcct acggaagatt atcaaaagga catctccacc tatattacga
    87901 aaaatccatc atagaaaaac agctttttca agtaaggatc ccgagcgata ttgcgcatgg
    87961 attacttctt gaaaaagctg ttattttagt tcaatttctg aatcaaattc tccaaacttt
    88021 cttttgcttc atctggcaag agatcttttc ctatctgagc ttcaatgaat cgtaaacgaa
    88081 tatcaagtcg ttgtttcacc agatttttat aggacgtatc ctcaaaagaa acaggaaaat
    88141 ctggtatagg taaatagtgg atgtcttcaa atggaccaaa cgacttgaat tctgccgttt
    88201 gttgcacgat tctttcaacg attgacaatg tcgtttcagg tttgattttt ttatcatcga
    88261 aatatccagt attcaggaca tagcaggctg tttgctgttt caaaaacaac tctcgaaaag
    88321 cttgatagtc ttccctaaga ggatagacac ggaaaggatt agcaaatggt tcaatgacta
    88381 gctcatctag ctgtcttttt gtagtgatat tttccgcgtc tgatcgtttt gttgctaatg
    88441 ttgcaccaaa agacgctgct aaaactgggt cattgatctt gataattggc ggaagcgaat
    88501 catctttcat gatccaaaaa acagcatcga tcttttcgct aagatgatcg actcggtcgg
    88561 gtgtcgcata acgtgatttg actgtccgcc catttccgtt tcgtatatct cctgttacta
    88621 ggactttttc tcctttactt gtacatgtca cgcccacatt ctgacaagta aggaaagaag
    88681 aaatggccgg atcatcagac ggatagtctt gtgttttatc aaagtaagaa ggttccaagg
    88741 cagtagcaaa accatttttt cgagaaataa cgaaagcatc gtcatgcaag atcgaaacag
    88801 agtgaccttc cgtatgtttt gctaaggtaa tcgttgattt tccagaaccg gacaacccaa
    88861 atacagccat cgtataggtc tggtcattac tagcatattg tttcatccca ccgtggcagg
    88921 caataaagcc attgcgattt cccaatgccc aagcaagcgt caaagtcgct ttttttaatt
    88981 ctccaaagta acgtaatcct agcacaaccg ctgtattatg gagagcatca aacaaaatga
    89041 gtccatttgg aaatgtttca tgtgtccatt cgggatcaca aaagatataa agatctccct
    89101 catcaaagga aacagacttt tgataagtct ctaagatctt cgaattcgct ggctgaaaat
    89161 tcagcaaata agaatacaaa ttgttctcaa atccttctgg aatcagcaaa tgactttgca
    89221 cagaaaattc ttcagatagg ccaacgatta cttttgtatg gtaaaactgg cgcttgcgac
    89281 tttgatacac agcttctctt aaaattttct cgtatttgct ttgttccttg tctaagtcct
    89341 caaagatccg ccgagcactt gctgttcttc ccacaatgct tccatcattg aatactagtg
    89401 tcctaacatc agctggtaaa tctagttctt cagcatgtga gattgttaaa tctgtgatga
    89461 tcgttcctgg actccctttt gctagttgat aagctgtctt cctatcaaaa acttcatgaa
    89521 cattattacc gtaaaatgtc gtttctacaa tcgatcgaat actggataac agtggatttc
    89581 cacttgtgat ttcgtttaat tcgagactct cttttgtact catggctcct cctcattccc
    89641 taaacaaaaa aacgttttct ttattataca ttattcgagt ttttatttgt actgctgcgc
    89701 tgtgggaagc aaacgagtta tttttcacac aaaaaaaccg agaacttgaa taaacaagtt
    89761 tctcggtttc atcattttcc ttttatcggc ttagtaccag ccgtttgcta accagaatgt
    89821 tttagcagca tcccatgaac cataacgaga tgttacatat tgttctgcta cacgttcttg
    89881 attggcagct gaatagtcgc cgtttaagta tgaagcatct aattgataac ggccgatgta
    89941 acgtccgtta gtagctgtgt atgaaccgcc tgattctttt tgtgcgatcc attcttttgc
    90001 agagcttgaa gttggcgctg ttgcagcagc tggggcagca ggagctactt caactgtttc
    90061 aacaggtgca acgtatgtat gttcttgcgt tgctgtttca gttgtagatg ctgtattttc
    90121 tgttgttttg ttttctgttg ttgctgaaga atcatttgaa gttgggattg ttaattgttc
    90181 accaacataa atgcggttga tgtttgcgat cgaatttttt tcagcaattg catcgatcat
    90241 tgagttgtct cctgcaaatt tttgtgagat cttagataat gaatcacctg attttactgt
    90301 ataaacttca tcagcgtgag catttgtagt tcctacaaac attgcagtag caccagcagc
    90361 aaaagttgta cctaaaacaa ttttcttaaa tgaattcata ttaaactcct ttttttctcc
    90421 gttttttatt ctttcgacgc tattaacttt atcatttaga aatatttcat aggtaaaaga
    90481 aacatgacat ttaatgacaa aataacgaat tatttaatat ttgtaacatt taaacaaata
    90541 tttttttgat acaaaaccat aaaagccttg ttgtataagc aaagtaatcg tttttctatg
    90601 ttttttttat ttttgaatga aaaacgcaga ttctgtaaca ttcactgcaa taatccataa
    90661 aaaaaagcaa aaaaattttt gatttttatc aaattctctt aaaaaaatgt aaaaaataca
    90721 aaaaaaccag taattgttac aacaattact ggacaaaaac tttgaataat gttaactatt
    90781 tttttatttt aactttctcc gcaataattt ctgggcatgt tccaattcgt ttataacgac
    90841 ttcttctgtt tctttagcta atatttcatc taatacatca agccattttt ccggttcttt
    90901 tgttcctaga cgtccaatag cccaagctgc tgttcctctg atgacagggc ggacatctgt
    90961 taaacattct gaaatctcag gcaatgccgt tcgatcacct agattagcta aagcaatcaa
    91021 tgcgtttctc tgcaaaggtt tcttcccacg ccatgaacca gctaaatgcc caaattgctg
    91081 tttgaagtct ttattagaca tcgaaagcat tggctttaat tttggatata tttcgtctac
    91141 ttttggttcc atctcttcat gaaaatgaaa atcttttcct tgattgtaag ggcagactaa
    91201 ctggcagata tcacatccat aaatgacatt tcgcattttt ttacgatatt cttccggcat
    91261 catccctttt gtctgagttt gataagacaa acatcgctgg gcattcattc ttccgtcacc
    91321 aagcaatgct tttgtcggac aagcagtcac gcagcgcata cagtcaccgc aaccaaattc
    91381 tcctggttta tcaggctcaa atacaatatt agtaacgatc tcaccaagat agacaaatga
    91441 accgaactct tcagtgatca acaatccgtt tcgtccaata aatcctaaac cagctcgctg
    91501 tgctacagcg acatcgatca gttctccagt atctacttgc ggagctagac gccattcctc
    91561 ttctttttgc catttttctg ctcttgtttg gatgaattcg atcaatcggt tcaaacggtc
    91621 tctgagtata tcatggtaat cgatccccca agaagcacga gcaaactgcc cgcgtttttc
    91681 atccctaggc acttcttcat ggatcctagt aggatacgca agtgcaatgg aaatgatcga
    91741 tttcggctga tcaaatgtca tttctggata aagacgctca tcgatatttg gatgttcaaa
    91801 gccagaagta tggccagctg ctttttgttc ttccaacgat tcttttaatg aatcaaaagg
    91861 ttccgccgtg gtgaacccga ttttgtcaat tcctaatcgt ttgctttctt gtatgatttc
    91921 ttcttttaat gtcatggtct tcctcgcttg ctgttaacgg ctgtataacc cataatatag
    91981 tttttctttt ttcagctgga caaactggat ctgctgatcg gcattcttct tttcttccat
    92041 ccattttttt cttgcagcaa atacttccca ataatttgtt gaaattaatt ttcttttgtt
    92101 atctaatgca ttaaaaaaat tcaatgaaac tggttttgta cttaacagtt catggagcct
    92161 tacctcttcc catggcgaaa actcagacag atcgcgggaa tacaatactt cttcatagag
    92221 atttttttgc tgtgtgtacg tgaatgtttg cgggattttc ccaggcctag caatttctac
    92281 tagacgatta taaaacatat tcattaattt gctgttggca ataaattgac ttttataata
    92341 ggccgactgg atctttttga ttactccttc tgctttgatg actgaaaata atgcaggcca
    92401 tgctttgacg gttatttcgt cgttttgttt aactaatacg ccaggtgtga agccgactgg
    92461 agaaaggaac acaaaataac cgataccggt attttcatct aaaggctgaa attcatcaaa
    92521 caaccatttg cctatgtaca gctcttcatc tggttctgtt gtctcatttt gatttgctat
    92581 gaataattta gctaattctg cttcaagaaa ttgaaggtct tgatccttgt cggtattttc
    92641 ctgctggctg atcaatcgaa agacttcttc tttaatcgta tatttatctt tacgtgtaaa
    92701 attcatagcc acaccttcct tccttactct aactataacg gaatttcttc aaaaaaggta
    92761 tctgtacgac gaaaaacagc ataatttttt ttcatatctc ttataaaaaa tgcaaggtct
    92821 gctcctcaag ctagaccttg cacttttcag ctttcttttg tttgtgacaa accttcatgg
    92881 atcgtttcaa tattccattt catcatctga tagtaagaat ctccttgttc tccttctttt
    92941 gctatggaat cagtgaacag tttatcatag attttcaaac ccgtttcttt tgctacccgt
    93001 tccatgcttc ttgagtctac actggtttcc acgaataaaa ccggtacttc ttttgctctt
    93061 atctgatcaa tgatcgcttt catttgatca ggcgtacctt gactttctgt attgatctcc
    93121 catatataag ctgctggcaa atcatatgct cgtgaaaaat acttgaatgc cccttcactt
    93181 gttactaata attttttgtt ctcttcgata gaagcaaaac tttccttcgc ttgtgtatct
    93241 aattctttta gtttttccac atatgcttct gcattttttt tatagagtgt cgcattttct
    93301 gcatcttttt cagagaatat acgtgcgatt tcctccacat attttattcc gttagatagg
    93361 tctagccaag catggggatc tgcttttgta tgttcttcac cgctagttaa ataaagaggt
    93421 tctacatttt tgctaactgc aaaataatct tttccttctt cttttttagc cgtttccatc
    93481 aagttatcga accagctgtt acctgtttca agattcaaac cgttgtataa aataacatct
    93541 gcatcgcttg cctttttgat gtcttctggt agtacttcat attcatgcgg atctgttccg
    93601 actggtacga tactgtggat atctatttga tctgttccga cctcttttgc catgtccgcc
    93661 aagatcgaat tggtagccac gactgacaac ttttctttac ttccttcatc cgcttggtca
    93721 gaagcctgct ttccgcaacc ggctaatacc aagaaactaa ctgccaagat taaaaagagt
    93781 gatttttttg ttttcatcta tctgcttcct ttctgaaata aaattccttt tgttggagaa
    93841 aataaaaaag caaggataaa aaatgcggta catgctaaca cgatcgtagc tcctgatggc
    93901 caattgtaac tataactgaa aaacacaccg acaactgcac ttagcatacc gattcctgca
    93961 gctgtaatga tcattttcag taaatggttc gttagcaagt aagccgtggc ggctggtgtg
    94021 atcaacatgg caatcaccaa gattgttcct actgtctgta aactgactac agccactaat
    94081 gtcaaaaaga acatcaaaag ataatggatc agcgatgtgt tcagcccata agcttttgcc
    94141 attgtcggat cgaatgatgt gatcttcaat tctttataaa agaaaaagac aaatatcaag
    94201 acgattccac taacgagtgc tgtcagcaac aaatcgcttt ccctaacagc taagacattc
    94261 ccaaataaaa tatgatacaa atctgtcgca ctctgggcat atgaaatcaa gataatccct
    94321 aatgcaaaaa aggagctaaa gacaattcca atcgctgtat catttttcaa tcggctccgc
    94381 tgagtcacga aaccaatcac tgctgctgtt agcatcccaa aagcagtggc tccaaagatg
    94441 taactaaaac caaacatata agaaatggct acacctggaa gaactgcgtg agagatagcg
    94501 tctcccatca aagacatccc tcgtaaaata ataaaagaac cgatgactcc agaagcaagt
    94561 cctacaagca tggaagtgac caaagcgtat tgcaggaatt gataattcat taatccatca
    94621 ataaaggatt ggatcatttt ttctctcctc cttttactaa atgtctgatg atctcaccat
    94681 aggccaattg aaggttcttc gatgtaaaga cttcttgtac tggaccggaa gcgatcagct
    94741 gtttatttaa aagaataacc tcatcgaaat attcttctac tttatgaaga tcatgatgga
    94801 cgatcaaaat cgtttttcct gaattcttca attcctgcaa gatgtcaaag atctttcgtt
    94861 cacttaacgc atcaatccct acgaatggtt catctaaaaa gatccattct gctccttggg
    94921 ctagagctct tgcaataaaa actctctgaa gctgtccacc cgatagttcg ctgatctgtc
    94981 ttttggcata ttcttccatc cctacttttt tcaaagcttg tcttgcacgt tctttttctt
    95041 ttttcccagg tctttgtcca attcgtaaag atggatatgt tcctaaaagt acaacgccta
    95101 atacatcaat tggaaaggaa agatccaatt cacttcgctg ttccacgtag gctatttttt
    95161 tacggactga gtttaccgac tgtccagaaa aggtgacttt accagtagta gctggtatca
    95221 actctagcaa agatttcata aatgttgatt ttcctgcacc gtttggtccg ataataccgg
    95281 taattttttg tggatttatc gtcaagttga tagttttcaa aacagttttc ccttgataag
    95341 cggctgaaag gtcttgaatt acaatggccg tacgttccaa atcttacact cctttttagg
    95401 tttacctaac tttttattta aatatagttt aactaaaatg gaaagcattt tcaagtagaa
    95461 tgatgaaaaa gtttttttcg aattttcctc gcattttatg aaaattttct gtagaatcac
    95521 atcaagagtt caataaggct aacctaataa tgaaaaacat gtgttgaaac gtttgattat
    95581 tttttcttgt tgactcaaca acgaaaggag tttttgtatg gaagaagaaa agttaactcg
    95641 aagtcttagt gcccgtcata tccaaatgat cgcactaggt ggaacgatcg gtgtcggtct
    95701 gtttatgggt gcctcctcaa ccatacgttg gacagggccc tcagtgatgg gagcctatgc
    95761 gattgcagga ttctttttgt atttgattat gcgtgcttta ggcgaaatgc tttacgttga
    95821 tccttcgact ggttcttttg caaattacgc gagcgagtac atccatcctg tggctggcta
    95881 cttgaccgct tggagtaata tttttcaata tattgtcgta ggaataagtg aagtgattgc
    95941 tgtaggatct tatatgaatt attggtggcc tgatttgccg gcaattattc ccggaatcat
    96001 tgtcgtcttg tttcttatgc tggcaaatct tatttctgtc aaggcgtttg gcgaattaga
    96061 attttggttt tcgatcatca aagtcataac gatcattttg atgatcattg caggtcttgg
    96121 ggtaatctta tttggatttg gtaatcatgg tcaggcagta gggatttcaa atctttggaa
    96181 aaatggtgga ttcttcactg gaggcgtcaa aggatttttc tttgcattat cgatcgttgt
    96241 tgcttcctat caaggaatcg aactgatcgg aatgactgct ggtgaagcag agaatcctaa
    96301 aaagacgatc attgaagctg tccagtccac tatcggtcga atcttgattt tttatattgg
    96361 tgctattttt atcattgtga gtatctatcc gtggaatcaa ttgagtgaga ttggttcacc
    96421 ttttgtacaa actttttcta aagtcggcat tacatttgct gcaggactga ttaattttgt
    96481 cgtgattaca gcggcattat caggttgcaa ttcagggatt ttcagcgcaa gtcgtatggt
    96541 ctatacactt gccataaatg gaaagatgtc taaaaaattt ctgaaactat ctcgtcacgg
    96601 tgcgcctttt tatcctgtcg tagcaatctc aggtggaatc ttaattggtc tgatcttaaa
    96661 ttatcttcta ccaatgttca tcaaccagtc agaaaatatt ttcgttttcg tatacagctc
    96721 aagtattttg cctggtatgg ttccttgggt agtgattttg atcagcgaga ttcgcttcaa
    96781 aaagaagcac gcagataaaa tgcatgatca tccttttaaa atgccactat ctccttatac
    96841 gaattatgtg acacttgctt ttttagctat cgtattgatt ttcatgttta tcaatcctga
    96901 aacgagaatc tcattgatta ttggactcgt tttcttgctt tacatgacga tccattactt
    96961 tgtacgcgaa aaaagaaccg gaaaataaaa aaagaacgaa gcttcacctt agtggaagct
    97021 tcgttctttt ttattgcttt tatcaattca attttagcgt ctcaccggga gtcaggaaat
    97081 aattctccat gctcatccca ttcagcgaca agatcgtttc tactggtacg ccagttcgtt
    97141 cggctatttg atttggacct tctccttcca aaactacggt tgtcgcagcc tgtgaagtcg
    97201 cgctgttggt attagtttca gaggaatctg ttgtgtcttg tgttgttcga gcttgcattt
    97261 cagaagcttc taccgactct gctgtctcag aatcagaaga tgttgtgtct tgtgtgcttg
    97321 ttgactctgc ttgaagagag gcgctagtct gcaaataaat atcggaaatc ttagcgacat
    97381 gattcgtata attagccgat aattgattga tcaaagtatc taagtctaca ccgtctttat
    97441 tttctgctgc ctgatacgct gcacgtaatt ttctgatccc ttcgtcggag attgcttgaa
    97501 gctcacgtgc tttttggtta gcaactgctg ataaaccagc tattccctct tgatttccct
    97561 gaatttctgt ctggtattct tcaatcaatg caggcgtctt ttcttcgatc tttttcgaat
    97621 aatcatttac gattgcttga agttgctctg gcaaaccatt tgctgttttt tcttcagaag
    97681 tttgtgtctc ttttgtttta gttgatgact cacttgtttg tttcgtctct gttttctttg
    97741 tcgttgtttc cgttatggtc gtacttttcg cttctgacga attttttcca tgaaaatata
    97801 gacccgcaac tcctcctgct atgatcaaaa acacggcaac tgctgcccca atcatgatca
    97861 tccgcttacg agctgcctgt ttttgctgtc ttcttcgggt catccgaaca ttttctcccc
    97921 actcattttc atcatacatt tttttcacca ccaattttta tgtataatag cgttatcaaa
    97981 cgcttcatat tcttattgta gcaaataatt gtcgctgtgc acatttttat ctttgttatt
    98041 tataagaaaa tttcaccagt tgagatgatt ccaagattgt ccgtcgattt ctccttgcag
    98101 ctgcattgtt cgttcgtcta tctcagccat cagtttcttt atttcatata agagcgcacg
    98161 gtcttgatat tctgattttt cgcttaattc atctaattgt tggatcagcc aatcatttgt
    98221 cataatgaag ttcctttcat ttcttgcagt gtaaaggtca agtcttcgat ctgctctttt
    98281 gcctcagtca gtgcttctac gatatatgct tgtttttcag attcttcttc ctgcattttc
    98341 tgattttcct gtactctttg aataaaccag tcaatccgct gagtcagcga tggtttgcct
    98401 tccaatgaga gatactgctg ataagcaatt agcttctccc aacgtttttc ctgggataaa
    98461 tacataaatt gtgctaaaac gaatggcgga agataggtca tatggaattt atttgcgatt
    98521 cgctgatacg gcctcaaaaa ttcggaaatc gtatattctt cttttcctcc agcttggtat
    98581 tctttttccg ccacacctgt tgtcaatacg attcctagtt ctttcccttt taatacatgt
    98641 gctgctcgtt ccaagacatt atcttgccaa atttttaaat gagcaggaga actataccag
    98701 taaaacggaa actggaaaat gatccgatca tgctgggcca agagctgtag ttcagtctct
    98761 atatctattt ttccgtctgg ataacttgat tccaaatgat gccaagtagc tggtaaacca
    98821 gcagaagctt ctttgaaaaa ttgctgattt aaagattctt cgatcgttgg atggctaatg
    98881 atgataagtg tacgcatccc ttttcctcct ttttagtctt cgctttacag tgtagcataa
    98941 ccgccacaag tttcttttgc cgatgcttgt cattttaacc agactaatca ggttgtccaa
    99001 gaaactgtgg tttgtttttc caactgtttt ttcaaggtat ccaaagcaat attcttaaaa
    99061 tttttgtttt tcatacgttt acgatagttc gtagccgttt ctccggtact ctttttgaac
    99121 attcggctaa atacgactag actattgaac cctacttgat aggcaatact ttccacggat
    99181 tcatccgtca gagaaagaag aaacgcccct cgattgatcc gtaccccgat caaatattcc
    99241 ttgatactgg tacccatatg atttttgaat actcgcgaca aatggctccg attgaccgct
    99301 aaatgctggg cgatttcctc aacagacaat ttttcagcat aatgtgcctc gatgtatttg
    99361 actgtttcaa cagctaaaga ggaatgaacc ttttgtacac caccgctgac ctttccgcca
    99421 tcttttaaaa ggactgataa aaaacggtag gttaatgctg ttaactgtaa ttcattttcc
    99481 aaggtttctt ccgtatcttt catgcattct aaaataatgt cgatatattt ttgtatgtca
    99541 gaagagacca tcgtgtattg gtcttctctg aaaagcgact gccgaatgat ttcttctgca
    99601 gcactcccac caaatgaaag ccagcaatac atccacggat cttctccatc tgataagtaa
    99661 aacgtcgagt ctcctggacg aatcaagaac agatcacctt ttttcaattg gaattgttga
    99721 tttttcacat gataagatcc tttgccttcc atcacgacat gcaggatgta atcattgcga
    99781 atagcaggac caaaagaatg aaacggcatc gttttagaga aaccacaaaa atcaaaatac
    99841 aattcttttt ctacttgttg atgttccttt tttaaaaaaa tcgattgctc cacgtctatc
    99901 cctccttact cctttagtat aatgcaacaa aaagttaaat aaaagtcaca gaaatatatt
    99961 aaaaaggaag cgatttcttg ttaagtttaa gtcattaaaa gatctttgtt attcgttgta
   100021 accggtttac ttagtattat atattgaaca ggagaggaat acagattatg aaaagcaaaa
   100081 tgatccaacg tgtgtcctac gctttaggcg cttttggaca cgacacattc tatgttacat
   100141 taagcactta ttttatggta ttcgtgacaa gccagatgtt cgcaggaaca gataaggcaa
   100201 cgaatgcaaa aatgattgct acagttacaa gcttagttgt aggtatccgc ttagttgaaa
   100261 ttatttttga tcctttgatt ggtggcatca tcgataatac tcgtacaaaa tacggtaaat
   100321 tcaaaccatg gttagttatc ggcggcgttg ttagttccgt actcttggta gcaatcttta
   100381 ccaatttctt tggcttagct actcataata cgactttatt tatcatatta tttgcaatcg
   100441 ttttcatctt attggatagt ttttattcat ttaaggatat cgctttttgg tcaatgattc
   100501 cagctttgag tacggaaaca aaggaacgtg aaaaatttgc tacgtttgct cgtttcggct
   100561 cttctctagg tgcaaatggt actactctag tcgttgtccc tatcgttact ttctttacct
   100621 atcttgttac tcataaacat attgaaggcg cttcaggctg gttctggttc ggtgtgattg
   100681 ctgctgttat ttcaggcggt actgcgatta tcactgcctt gggcacaaaa gaagtcgata
   100741 ctgcgatccg tcagcaaaat gaaaaagctg ggatcaaaga tgtgttcaaa gcagttgctc
   100801 aaaatgatca gttgatgtgg cttgcccttg cttatatcgc ttatgcgatt gccaatgcag
   100861 caacaactgc cgtattattc tacttcttca aatatgtact tggccaacca actgaatttt
   100921 ggatcgttgg tctagttgct gccttacttg gtttagtagc tgttccacta ttcccaatgc
   100981 ttacacgcgt catctctcgt cgctacgttt accttggagg gatcgcaatg atgatttctt
   101041 cttacttttt ctttacgatc gcaggttcaa gtttgccaat cgttatcatc gggctagtat
   101101 tgttctactt ccctcaacaa ttgattttct tatccgcttt gatgacgatc acagactctg
   101161 ttgaatatgg tcaatggaaa aatggtatcc gtaacgaagc tgttactcta tcaattcgcc
   101221 ctttactaga taaaatcgct ggtgctttgt ctaacgggat cgtagggttt gtcgctatcg
   101281 cttctgggat gactggtaat gctactgctg ctgatatcac tgctcatggc gtaacgactt
   101341 tcaaagctta tgcattctat gctcctgctg ctttgatgat tttctccgct attatttttg
   101401 cttggaagat tacattgaca gagaaaaaac atgcacaaat cgttgagaaa ttggaaaaca
   101461 aattggctcc agctgataca atggaagatg aacttcacga tgcagtagat ccttcccatt
   101521 aagaaaggat cttgaaaatg aagattacag aaaaagattt tggccaaggt tatcacttga
   101581 taaccttgga gaacaaaaac aagctagcac tttctatctc tgatttaggt gctagaatcg
   101641 tcagtttaaa atcaaatgat cgagaattag ttttgggatt tgacaccgct gaagaatata
   101701 tcgaaaaaga tccgtatatc ggtgcttcta tcggtcgcac cgcagggaga atcgaaaatg
   101761 gccacttttc tctcaatgga aaaacttacc aattagccac agatccaaaa actggacata
   101821 atctgcatgg tggtgctcct ggatttgaat taaaaaaatg gtcttatgtc attttgaatg
   101881 gcgaaaatga agcttctgtc atttttacta ctacaagtcc cgacggagaa catggttttc
   101941 caggtacaat ggatgtggaa atccgctata ctttgacaaa agataatatc tggcgagtga
   102001 cctctcgcgg caccagtgac caagatactt tattcaatcc gacaaatcat gtttacttca
   102061 acttgacggg agatgcttct cagtcaatcg atcaacatga gctttggctc aatagcgaag
   102121 cttatgctcc tttgcgaaca gatagtattc ctataggggt aaaagaaaat gctgcaggaa
   102181 gtgctttcga ttttcaaata cctaaaaaat tagcgtccgt ttttgctagt gatttggatc
   102241 aaaaaaatct tgttgacgga atcgatcatc catttttctt aaaagaaacc ggcttgggta
   102301 aagaagccgc tcgcttgact agccccgata aaagaatcca agtagatatt gctacagatg
   102361 cttctagtgt ggttattttc actgcaaatt tcggcacaga aacaccagaa atgcgcaacc
   102421 gaaaactggc acatcacggt ggcatcacat ttgagactca aacagctcca ggtgcagagc
   102481 gcttctcagc atttggttcg attcatttaa aagccggatc agtatttgaa acagtgacag
   102541 aattcaaaat caaaacaaga aaggaataac tcatcatgga aatcaaaaga agcttaaaca
   102601 caaaatttgc cgaaacattc ggacctaaaa aaactacaca atacttctca ccaggacgca
   102661 tcaatttgat tggtgaacac acagattaca atggaggcca tgtcttcccc gcttccatca
   102721 cttatggaac gcaaggtgta gcagcacctc gagacgataa taaaatatta gtttattcca
   102781 caaactttga cgatatcggt gtcattagtt ttactttaga tgagctagaa tatgataaaa
   102841 aagcaaactg ggcaaattac gtaaaaggaa tggtccttaa attaaaagaa gctggacact
   102901 caatcgatca tgggtttgag ttattagtgg aaggaacaat ccctaatggt gcaggacttt
   102961 ccagtagcgc ttcattagaa ttattagtag gtgttgtatt agaagatctg tttgatttaa
   103021 aaacagatcg cttggaacta gtccaaacag gtaaaaaagt agaaaatgaa ttcatcggtg
   103081 tcaattcagg tatcatggat cagtttgcca tcggcttcgg tgagctagat catgcaatct
   103141 tattagatac aaatacgctg aaatacgaaa tggttccagt gaaactagat ggttatgcaa
   103201 tcgtcatcat gaatacgaat aagcggcggg aattggcaga ttcaaaatac aatgaacgcc
   103261 gtagcgaatg cgaagaagcc ttggcacgtt tgcaaaaaac tttgcctatc tcttctttag
   103321 gagacttaga tgaagaagaa tttgaatcaa ctattgacca aatcggcgat gatacattga
   103381 tacgcagagc tcgccatgcc gtttctgaaa atcagcggac tttgaaggca aaagctgaat
   103441 tagaagctgg gaacttagaa gcatttggtc aactgttaaa tgattctcac cactctcttc
   103501 gctatgatta tgaagtaact gggatcgaat tagatacact agtagatgct gctcaaaaac
   103561 aagaaggtgt cttaggtgca cgaatgacag gagctggatt tggtggctgt gcgattgctc
   103621 ttgtcaaaga agaaaatata cctgccttca aaaacaatgt ctatgatgaa tacatgaaag
   103681 ttgtcggcta tgctccggaa ttttatgttg cacacattgg aaatgggaca acaaagatcg
   103741 atccagaagc tgttcatgtt tgataaaaaa atcaacgatc tcagctaaaa acaaatagat
   103801 aaaaataacc tctcagcaat ttccaaatgt gagccttttg ctcgtatttg aaaattgctg
   103861 ttttttattg acacggaaag agaacgtgtg ttctaattgt gttgaagagg tgattgttat
   103921 gtgggcgaat caaaatccgg tatttgttta tgaaaaagaa ccttcaagag atattttatg
   103981 tattgactgc aaatcttttt acgcctccgt agagtgcgtt gaaaaggatc ttgatcccct
   104041 tacaactaaa ttagtagtca tgtcctatcc gtccgattcg acagaaacac ggggtagcgg
   104101 attaatcttg gcttccagtc ctgcagcaaa aaaagcttac ggcatcacca atatcagccg
   104161 tgccagagat cttcccttcc cctatccaag tgatttatat attgttgccc caaggatggc
   104221 ctattatatg gaaaagaaca gagaaatcaa ttctatctat aaaaaatatg cagacgaagc
   104281 gaaccatcat gtctattccg tcgatgaaag cttccttgat gtaacaaata gcatggcact
   104341 ctttggtaaa acaaatgcgt acgacatggc taaactgatc cataaagatg tgtttgagca
   104401 gaccggaatc tacacgacta taggaattgg cgataacccg ctacttgcta aactagcctt
   104461 agacaatggc tcaaaagacg gaaagcaaat gtttgctgaa tggcgttatg aagatgtacc
   104521 agaaaagtta tggaaaatct cagatatcac tgacttttgg ggaatcggca aacgaacagc
   104581 aaaacgtctg aaacaaatgg gtatacattc tgtttatgat ttagctcacg cggattatta
   104641 tcacttgaaa agcaagctag gggtcatcgg tactcagctt tatgcccatg cttggggcat
   104701 cgatcgaagt tttttgggac aagaatatac accaaaatca aaaagtatcg gaaatagtca
   104761 aatcctagat agagattata caagacgaga agaaatcgag atcgttatca aagaaatggc
   104821 cgaccaagtc ggcactcgct tgagacgatc tgcagcaaaa acacaagtag tcagcttatg
   104881 ggtaggatac tctttaggct atcttgatcc aaatggcaag accgggtttc accagcaaat
   104941 gaaaatcgaa gcgacaaatg ctagcagaga tctagcagat gctttattga ccattttcca
   105001 ccagcattac cactaccagg atattcggcg tgtaggcgtg aactgttccg acctcgtata
   105061 cgcgacaggt ttacagctta acctttttga agatcctgaa aagcagataa atgaagcgaa
   105121 agttgatttt gttgtcgata cgattagaaa aaaatacggc tttcaatcaa tcatccatgc
   105181 gcatagtctc ttgcaaggag gacgcgccat cgcacgtagt tctctcgttg gcgggcatgc
   105241 aggtggtatg agtggattag aaggtggaaa agatgagacg gacagcaaaa aaattcattg
   105301 actataacga gtatcgagat cggccatttg gattaaaatg ggggactgct tttgcgatgg
   105361 aagaactagt caacggtatc aaaaaaaatg aagaagaagc acgcaaacag cctcttccta
   105421 aacaacaaat gacattgcaa gaaatcgata gtatactgac agaagccttt ttatctcgta
   105481 aagcagtaat cattcagctc aatttaaaag atgaatttgg aagactcttg gatgaggtcg
   105541 aagggcactt cattggtgaa gcctatgaag attactttat gattgaccat cagccgatat
   105601 tgtgggaaga cgtacggtat attgagttaa aggaaacaaa aaaatggttt gtgacatgag
   105661 aggttgtgac agaagtgatc aacttcaaaa aataagaagg gatttttcga aaattgcttc
   105721 ttaaattttt gtggaatttt ggcttatttt tcgaagaagc aacttctgga acaacgtata
   105781 ttagatatca gaggttgtga aaaagtcgct tagttttgag taacaagcca aggcgctgat
   105841 ttcacaacct ctgtctatct atccatctat tcggtaagat tcatgcatga cgtcttcttt
   105901 atttagtatg tagagccctt tttcttctga ccattcaaca taaatcagct gttttacatc
   105961 tgcatagcct tctttcttca agttctccat aagccatgct tcagtcaatt gattggactg
   106021 aagcacttta tgctgaattt gcccttcatc tacaagcagg atcgataaag ctttgtcttc
   106081 atctgatttt ttcaatacac tcatttgacc ggcattctct aggataacat gtttggcatt
   106141 ttcaagaaaa aagcattctt gctgtcttaa gattgctcgc aactgctcca tctcgatatg
   106201 gttatttgtc aactctgtca tgttgatgac gccgtctttg attagaacag atggctcgcc
   106261 tttgacccaa cgattgacct tttcattttt ttggacgata cgttcgatac cataaatcat
   106321 tactgcccaa agcgcaatgg caaataggac atgccctaca tggacattgt catcataggt
   106381 tgattcttct aagatcccac ctaaaacaag cgtatagact aaatcaaacg gtgtgatttc
   106441 tgacatggat ttcttcccaa gcaaacgtac cactaataat aaaccgatca acccgaacac
   106501 cagtttcatt gtaattgaaa aaatcatttt ttctccccct aactatctgt taattaaatt
   106561 atatatgctt tctcacgaac acgcatggaa tatacaagga agaaatgtat ccttttacta
   106621 aaaaaaggac tgtgacactg tagtgacccc caaaagttag acttttttgg agtggagaaa
   106681 tccactcctt ttttttatgc tctaaaatga tatttttaca caaccgttac ggttgtttta
   106741 cgaaattgga cg
//
LOCUS       DAFEOM010000003        96869 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.003, whole genome shotgun sequence.
ACCESSION   DAFEOM010000003
VERSION     DAFEOM010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 96869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 96869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..96869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.003"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(25..1647)
                     /locus_tag="LP524_000200"
     CDS             complement(25..1647)
                     /locus_tag="LP524_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL2233283.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            1896..2417
                     /locus_tag="LP524_000201"
     CDS             1896..2417
                     /locus_tag="LP524_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBL2233284.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(2603..4333)
                     /gene="spxB"
                     /locus_tag="LP524_000202"
     CDS             complement(2603..4333)
                     /gene="spxB"
                     /locus_tag="LP524_000202"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HBL2233285.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            4498..5316
                     /locus_tag="LP524_000203"
     CDS             4498..5316
                     /locus_tag="LP524_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBL2233286.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(5419..5616)
                     /locus_tag="LP524_000204"
     CDS             complement(5419..5616)
                     /locus_tag="LP524_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233287.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            5785..6363
                     /locus_tag="LP524_000205"
     CDS             5785..6363
                     /locus_tag="LP524_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBL2233288.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            6458..6595
                     /locus_tag="LP524_000206"
     CDS             6458..6595
                     /locus_tag="LP524_000206"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233289.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(7408..7911)
                     /locus_tag="LP524_000207"
     CDS             complement(7408..7911)
                     /locus_tag="LP524_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBL2233290.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            8270..9295
                     /locus_tag="LP524_000208"
     CDS             8270..9295
                     /locus_tag="LP524_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL2233291.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            9642..10520
                     /locus_tag="LP524_000209"
     CDS             9642..10520
                     /locus_tag="LP524_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HBL2233292.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            10996..11112
                     /locus_tag="LP524_000210"
     CDS             10996..11112
                     /locus_tag="LP524_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBL2233293.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            12040..12378
                     /locus_tag="LP524_000211"
     CDS             12040..12378
                     /locus_tag="LP524_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233294.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETETILTDYSKI
                     ATDKKYILWM"
     gene            12472..12702
                     /locus_tag="LP524_000212"
     CDS             12472..12702
                     /locus_tag="LP524_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233295.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            13102..13473
                     /locus_tag="LP524_000213"
     CDS             13102..13473
                     /locus_tag="LP524_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HBL2233296.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(13747..14439)
                     /locus_tag="LP524_000214"
     CDS             complement(13747..14439)
                     /locus_tag="LP524_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL2233297.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(14442..15227)
                     /locus_tag="LP524_000215"
     CDS             complement(14442..15227)
                     /locus_tag="LP524_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL2233298.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            15464..16864
                     /gene="lacG"
                     /locus_tag="LP524_000216"
     CDS             15464..16864
                     /gene="lacG"
                     /locus_tag="LP524_000216"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HBL2233299.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            16889..17206
                     /locus_tag="LP524_000217"
     CDS             16889..17206
                     /locus_tag="LP524_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBL2233300.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            17219..18892
                     /locus_tag="LP524_000218"
     CDS             17219..18892
                     /locus_tag="LP524_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HBL2233301.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            18906..20537
                     /locus_tag="LP524_000219"
     CDS             18906..20537
                     /locus_tag="LP524_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HBL2233302.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            21409..22599
                     /locus_tag="LP524_000220"
     CDS             21409..22599
                     /locus_tag="LP524_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HBL2233303.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            22733..24922
                     /locus_tag="LP524_000221"
     CDS             22733..24922
                     /locus_tag="LP524_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL2233304.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            24919..26712
                     /locus_tag="LP524_000222"
     CDS             24919..26712
                     /locus_tag="LP524_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBL2233305.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            26912..27226
                     /locus_tag="LP524_000223"
     CDS             26912..27226
                     /locus_tag="LP524_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL2233306.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            27247..27624
                     /locus_tag="LP524_000224"
     CDS             27247..27624
                     /locus_tag="LP524_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL2233307.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            27634..28407
                     /locus_tag="LP524_000225"
     CDS             27634..28407
                     /locus_tag="LP524_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233308.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            28429..29832
                     /locus_tag="LP524_000226"
     CDS             28429..29832
                     /locus_tag="LP524_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBL2233309.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            30014..31198
                     /locus_tag="LP524_000227"
     CDS             30014..31198
                     /locus_tag="LP524_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBL2233310.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            31195..31485
                     /locus_tag="LP524_000228"
     CDS             31195..31485
                     /locus_tag="LP524_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233311.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            31482..31703
                     /locus_tag="LP524_000229"
     CDS             31482..31703
                     /locus_tag="LP524_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233312.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            31745..32524
                     /locus_tag="LP524_000230"
     CDS             31745..32524
                     /locus_tag="LP524_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HBL2233313.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            32591..33085
                     /locus_tag="LP524_000231"
     CDS             32591..33085
                     /locus_tag="LP524_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBL2233314.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            33141..33779
                     /locus_tag="LP524_000232"
     CDS             33141..33779
                     /locus_tag="LP524_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233315.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            33851..34246
                     /locus_tag="LP524_000233"
     CDS             33851..34246
                     /locus_tag="LP524_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL2233316.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            34230..36683
                     /locus_tag="LP524_000234"
     CDS             34230..36683
                     /locus_tag="LP524_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL2233317.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            36680..38707
                     /locus_tag="LP524_000235"
     CDS             36680..38707
                     /locus_tag="LP524_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233318.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            38704..39726
                     /locus_tag="LP524_000236"
     CDS             38704..39726
                     /locus_tag="LP524_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBL2233319.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDGETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            39743..40672
                     /locus_tag="LP524_000237"
     CDS             39743..40672
                     /locus_tag="LP524_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL2233320.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            40917..41033
                     /locus_tag="LP524_000238"
     CDS             40917..41033
                     /locus_tag="LP524_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBL2233321.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            41049..42968
                     /gene="tet(M)"
                     /locus_tag="LP524_000239"
     CDS             41049..42968
                     /gene="tet(M)"
                     /locus_tag="LP524_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBL2233322.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            43066..43254
                     /locus_tag="LP524_000240"
     CDS             43066..43254
                     /locus_tag="LP524_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBL2233323.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(43312..43665)
                     /locus_tag="LP524_000241"
     CDS             complement(43312..43665)
                     /locus_tag="LP524_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2233324.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            44196..44618
                     /locus_tag="LP524_000242"
     CDS             44196..44618
                     /locus_tag="LP524_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL2233325.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            44615..44845
                     /locus_tag="LP524_000243"
     CDS             44615..44845
                     /locus_tag="LP524_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2233326.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            45343..45543
                     /locus_tag="LP524_000244"
     CDS             45343..45543
                     /locus_tag="LP524_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBL2233327.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            45570..46763
                     /locus_tag="LP524_000245"
     CDS             45570..46763
                     /locus_tag="LP524_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL2233328.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(46831..48396)
                     /gene="guaA"
                     /locus_tag="LP524_000246"
     CDS             complement(46831..48396)
                     /gene="guaA"
                     /locus_tag="LP524_000246"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBL2233329.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(48455..49378)
                     /locus_tag="LP524_000247"
     CDS             complement(48455..49378)
                     /locus_tag="LP524_000247"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBL2233330.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            49574..50179
                     /locus_tag="LP524_000248"
     CDS             49574..50179
                     /locus_tag="LP524_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL2233331.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            50487..51503
                     /locus_tag="LP524_000249"
     CDS             50487..51503
                     /locus_tag="LP524_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBL2233332.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            51644..52945
                     /locus_tag="LP524_000250"
     CDS             51644..52945
                     /locus_tag="LP524_000250"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBL2233333.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            52991..53653
                     /gene="deoC"
                     /locus_tag="LP524_000251"
     CDS             52991..53653
                     /gene="deoC"
                     /locus_tag="LP524_000251"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBL2233334.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            53674..54072
                     /locus_tag="LP524_000252"
     CDS             53674..54072
                     /locus_tag="LP524_000252"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBL2233335.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            54138..55220
                     /locus_tag="LP524_000253"
     CDS             54138..55220
                     /locus_tag="LP524_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL2233336.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            55432..56994
                     /locus_tag="LP524_000254"
     CDS             55432..56994
                     /locus_tag="LP524_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2233337.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            56987..58120
                     /locus_tag="LP524_000255"
     CDS             56987..58120
                     /locus_tag="LP524_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2233338.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            58120..59076
                     /locus_tag="LP524_000256"
     CDS             58120..59076
                     /locus_tag="LP524_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2233339.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            59175..60341
                     /gene="deoB"
                     /locus_tag="LP524_000257"
     CDS             59175..60341
                     /gene="deoB"
                     /locus_tag="LP524_000257"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBL2233340.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            60365..61183
                     /locus_tag="LP524_000258"
     CDS             60365..61183
                     /locus_tag="LP524_000258"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBL2233341.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            61200..61907
                     /gene="deoD"
                     /locus_tag="LP524_000259"
     CDS             61200..61907
                     /gene="deoD"
                     /locus_tag="LP524_000259"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBL2233342.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(62205..62891)
                     /gene="gpmA"
                     /locus_tag="LP524_000260"
     CDS             complement(62205..62891)
                     /gene="gpmA"
                     /locus_tag="LP524_000260"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBL2233343.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(63063..63644)
                     /locus_tag="LP524_000261"
     CDS             complement(63063..63644)
                     /locus_tag="LP524_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL2233344.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(63777..64460)
                     /gene="rpiA"
                     /locus_tag="LP524_000262"
     CDS             complement(63777..64460)
                     /gene="rpiA"
                     /locus_tag="LP524_000262"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBL2233345.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            64779..65192
                     /gene="rpsL"
                     /locus_tag="LP524_000263"
     CDS             64779..65192
                     /gene="rpsL"
                     /locus_tag="LP524_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBL2233346.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            65276..65746
                     /gene="rpsG"
                     /locus_tag="LP524_000264"
     CDS             65276..65746
                     /gene="rpsG"
                     /locus_tag="LP524_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBL2233347.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            65840..67927
                     /gene="fusA"
                     /locus_tag="LP524_000265"
     CDS             65840..67927
                     /gene="fusA"
                     /locus_tag="LP524_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBL2233348.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            68099..69286
                     /gene="tuf"
                     /locus_tag="LP524_000266"
     CDS             68099..69286
                     /gene="tuf"
                     /locus_tag="LP524_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBL2233349.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            69693..70001
                     /gene="rpsJ"
                     /locus_tag="LP524_000267"
     CDS             69693..70001
                     /gene="rpsJ"
                     /locus_tag="LP524_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBL2233350.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            70032..70661
                     /gene="rplC"
                     /locus_tag="LP524_000268"
     CDS             70032..70661
                     /gene="rplC"
                     /locus_tag="LP524_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBL2233351.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            70689..71312
                     /gene="rplD"
                     /locus_tag="LP524_000269"
     CDS             70689..71312
                     /gene="rplD"
                     /locus_tag="LP524_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBL2233352.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            71312..71602
                     /gene="rplW"
                     /locus_tag="LP524_000270"
     CDS             71312..71602
                     /gene="rplW"
                     /locus_tag="LP524_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBL2233353.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            71641..72474
                     /gene="rplB"
                     /locus_tag="LP524_000271"
     CDS             71641..72474
                     /gene="rplB"
                     /locus_tag="LP524_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBL2233354.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            72517..72795
                     /gene="rpsS"
                     /locus_tag="LP524_000272"
     CDS             72517..72795
                     /gene="rpsS"
                     /locus_tag="LP524_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBL2233355.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            72818..73165
                     /gene="rplV"
                     /locus_tag="LP524_000273"
     CDS             72818..73165
                     /gene="rplV"
                     /locus_tag="LP524_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBL2233356.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            73179..73835
                     /gene="rpsC"
                     /locus_tag="LP524_000274"
     CDS             73179..73835
                     /gene="rpsC"
                     /locus_tag="LP524_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBL2233357.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            73838..74272
                     /gene="rplP"
                     /locus_tag="LP524_000275"
     CDS             73838..74272
                     /gene="rplP"
                     /locus_tag="LP524_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBL2233358.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            74262..74450
                     /gene="rpmC"
                     /locus_tag="LP524_000276"
     CDS             74262..74450
                     /gene="rpmC"
                     /locus_tag="LP524_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBL2233359.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            74475..74741
                     /gene="rpsQ"
                     /locus_tag="LP524_000277"
     CDS             74475..74741
                     /gene="rpsQ"
                     /locus_tag="LP524_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBL2233360.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            74799..75167
                     /gene="rplN"
                     /locus_tag="LP524_000278"
     CDS             74799..75167
                     /gene="rplN"
                     /locus_tag="LP524_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBL2233361.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            75204..75512
                     /gene="rplX"
                     /locus_tag="LP524_000279"
     CDS             75204..75512
                     /gene="rplX"
                     /locus_tag="LP524_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBL2233362.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            75540..76079
                     /gene="rplE"
                     /locus_tag="LP524_000280"
     CDS             75540..76079
                     /gene="rplE"
                     /locus_tag="LP524_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBL2233363.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            76098..76283
                     /locus_tag="LP524_000281"
     CDS             76098..76283
                     /locus_tag="LP524_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBL2233364.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            76319..76717
                     /gene="rpsH"
                     /locus_tag="LP524_000282"
     CDS             76319..76717
                     /gene="rpsH"
                     /locus_tag="LP524_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBL2233365.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            76749..77285
                     /gene="rplF"
                     /locus_tag="LP524_000283"
     CDS             76749..77285
                     /gene="rplF"
                     /locus_tag="LP524_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBL2233366.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            77446..77802
                     /gene="rplR"
                     /locus_tag="LP524_000284"
     CDS             77446..77802
                     /gene="rplR"
                     /locus_tag="LP524_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBL2233367.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            77823..78323
                     /gene="rpsE"
                     /locus_tag="LP524_000285"
     CDS             77823..78323
                     /gene="rpsE"
                     /locus_tag="LP524_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBL2233368.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            78338..78517
                     /gene="rpmD"
                     /locus_tag="LP524_000286"
     CDS             78338..78517
                     /gene="rpmD"
                     /locus_tag="LP524_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBL2233369.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            78561..79001
                     /gene="rplO"
                     /locus_tag="LP524_000287"
     CDS             78561..79001
                     /gene="rplO"
                     /locus_tag="LP524_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBL2233370.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            79001..80296
                     /gene="secY"
                     /locus_tag="LP524_000288"
     CDS             79001..80296
                     /gene="secY"
                     /locus_tag="LP524_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBL2233371.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            80358..81005
                     /locus_tag="LP524_000289"
     CDS             80358..81005
                     /locus_tag="LP524_000289"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBL2233372.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            81196..81414
                     /gene="infA"
                     /locus_tag="LP524_000290"
     CDS             81196..81414
                     /gene="infA"
                     /locus_tag="LP524_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBL2233373.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            81447..81563
                     /gene="rpmJ"
                     /locus_tag="LP524_000291"
     CDS             81447..81563
                     /gene="rpmJ"
                     /locus_tag="LP524_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBL2233374.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            81581..81946
                     /gene="rpsM"
                     /locus_tag="LP524_000292"
     CDS             81581..81946
                     /gene="rpsM"
                     /locus_tag="LP524_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBL2233375.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            81974..82363
                     /gene="rpsK"
                     /locus_tag="LP524_000293"
     CDS             81974..82363
                     /gene="rpsK"
                     /locus_tag="LP524_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBL2233376.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            82443..83381
                     /locus_tag="LP524_000294"
     CDS             82443..83381
                     /locus_tag="LP524_000294"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBL2233377.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            83409..83789
                     /gene="rplQ"
                     /locus_tag="LP524_000295"
     CDS             83409..83789
                     /gene="rplQ"
                     /locus_tag="LP524_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBL2233378.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            84061..84900
                     /locus_tag="LP524_000296"
     CDS             84061..84900
                     /locus_tag="LP524_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL2233379.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            84876..85745
                     /locus_tag="LP524_000297"
     CDS             84876..85745
                     /locus_tag="LP524_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL2233380.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            85735..86532
                     /locus_tag="LP524_000298"
     CDS             85735..86532
                     /locus_tag="LP524_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBL2233381.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            86735..87481
                     /gene="truA"
                     /locus_tag="LP524_000299"
     CDS             86735..87481
                     /gene="truA"
                     /locus_tag="LP524_000299"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBL2233382.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(87814..90483)
                     /locus_tag="LP524_000300"
     CDS             complement(87814..90483)
                     /locus_tag="LP524_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HBL2233383.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(90721..90909)
                     /locus_tag="LP524_000301"
     CDS             complement(90721..90909)
                     /locus_tag="LP524_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBL2233384.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            91179..91541
                     /locus_tag="LP524_000302"
     CDS             91179..91541
                     /locus_tag="LP524_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBL2233385.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            91593..93275
                     /locus_tag="LP524_000303"
     CDS             91593..93275
                     /locus_tag="LP524_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBL2233386.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            93392..95428
                     /gene="recG"
                     /locus_tag="LP524_000304"
     CDS             93392..95428
                     /gene="recG"
                     /locus_tag="LP524_000304"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBL2233387.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            95477..96478
                     /gene="plsX"
                     /locus_tag="LP524_000305"
     CDS             95477..96478
                     /gene="plsX"
                     /locus_tag="LP524_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBL2233388.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            96600..96842
                     /gene="acpP"
                     /locus_tag="LP524_000306"
     CDS             96600..96842
                     /gene="acpP"
                     /locus_tag="LP524_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBL2233389.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT        31569 a        16930 c        20066 g        28304 t
ORIGIN      
        1 ttatttttta agctaaatag gccattatgc ccataactcg ttcaattgtt tttgaatatc
       61 tgcattttcc aaaaattcat catatgtcgt atcagctcga tcgatcacac ctttatccga
      121 aacggcaatg atgcggtttg ccaatgtttg gataaattga tggtcatgtg acgcaaataa
      181 gattgagcca gtaaatgcca tcaagccgtc attcaatgct gtgattgatt ctaagtctaa
      241 atggttcgtt ggatcatcta atacaagtac gttagctttc gaaagcatca tttttgaaag
      301 catgcaacgg actttctctc caccagataa aacattcact ggtttcaata cttcttcacc
      361 agaaaacaac attcttccta agaagcttct taagaaggtg ttatcatctt cttctttact
      421 tgcatattga cgcaaccagt ctaaaatcgt tagatcagta tcgaattctt tcgttgtatc
      481 ctttggtaaa taagaattac ttgttgtcac accccaacgt actgttccag tatctggcgt
      541 aatttcaccc atgataactt tgaatagcgt tgttgttgtt atatcatttt ctgcgacgaa
      601 agcgaccttg tcttctttgc tcaaattaaa agatatatca tctaaaattt tctttccatc
      661 aatcgttaca cttacatttt ctacctgcaa taagtcgttt ccaatctcac gttcaggttt
      721 gaatccaacg aatggataac gtctagaaga aggttggata tcgtctaaag taattttttc
      781 aagcattttt ttacgtgaag ttgcttgttt tgattttgat gcattcgcac taaagcgggc
      841 aataaattct tggagttctt tgatttgttc ttcttttttg gcatttgcgt ttgcttgaag
      901 tttagccgct agttgactag attccaacca aaaatcataa tttcctacat aaagtttgat
      961 tttaccaaaa tctagatccg ccatatgtgt acatactttg ttcaaaaagt gacggtcatg
     1021 ggatacaacg atcacagtat tttcaaaatt gatcaaaaat tcttccagcc aattgatcga
     1081 ttggatatcc agaccgtttg ttggttcgtc tagcaaaaga acatcaggtt taccaaatag
     1141 tgtttgtgct aataacactt ttaccttttg tccaccagtt aattcactca tttgctgatg
     1201 gtgcaattct tcagggatgt tcagaccttg cagtaaaaca gctgcttctg gttcagcttc
     1261 ccatccatta agctcagcaa attctccttc taattcagct gctcgaatac catcttcgtc
     1321 agtgaaatcc tctttcatgt aaatcgcatc tttttctttc agcacttcat acagacgctt
     1381 gtgtcccatg atgactgttt caagtacagt atagtcttca tagtcgtaat ggttctgttt
     1441 caatacggcc atccgttcat ctggtcccat agtaacggaa cctgtggaag gctgcagatc
     1501 ccctgataac actttaagga acgttgattt acctgcacca tttgcaccaa tcaagccata
     1561 gcagtttccg ggggtaaatt taatattcac atcatcaaat agtttgcggt ctgaaaagtg
     1621 caaactcaca tcatttacag taatcaattt ttttcctcac ttttatcatt attagttttg
     1681 acaagtctta tagaattata taggggaaag gtaaaagatt caaggcaatc gaacattctt
     1741 tgtgtttcac gtgaaacaga atgaaaaaaa taaaaataaa agcggagcga aaatagctac
     1801 tataggattt attagtttgc aacagcataa tttttgacgt tatttataag agggagtact
     1861 ataaaactgt aaaagaaatg aaagaaggat tgaaaatgga tgaaaaaaga accatcgact
     1921 ggggctcatt gattttggga attttatttg tacttgtctc attgttatca tttcaagacc
     1981 ctgtcggaaa tttagttgca attgtagtag tctttgcgat ttttgccttt attaaaggga
     2041 tatttgaact gtttgtacgg aacagattaa aagagctaac aggatataaa ggaaagatgc
     2101 ccttaataat cgggatcatc gatattctag ttggcgtctt tttcttattc aatattggtg
     2161 caggagtcgc agcattgcct tttgtttttg cagtatggtt tattgctgat tcagttttag
     2221 cgttatttac tgctgacttg gcaagaggag taagtgaagg atattattgg tttacgatca
     2281 tcgtcaatat tttaggaatt ttattaggaa ttttcttatt gtttaatcca atttcttctg
     2341 cgttgacatt aagcttttta gttggattct attttatgtt atttggtatc acacatatcg
     2401 tctatgcatt tagataagta aaatcaaaca aataaaaaag ccggcttccg attacagttt
     2461 tactgttgta attggaagcc ggctttttta tcatggacaa atatgcacga ggtgattttt
     2521 aggtgtcgag acaaatttat gcaaaaatgt gagaagctat atacacaaat tttatcttaa
     2581 tactcgaagc atagcgcagt tgtcacaacc tcttttcata taattcattc aatactggca
     2641 aatcatggac ttcatatttt tctttgaatg cttggatttc ttcttcagaa tattttcttg
     2701 gatctaaatg cagatcttct actggcaatg ggcgcttgtt attgatcttc acatcgatta
     2761 ctacaggacg atctgatttt gcagctgcat caaaggcaaa tcgaagttgc gagtattctg
     2821 tgactgtaaa gctatttgcc ccgagcgctt ctccaacttt accgaaatct gcatcttcta
     2881 aaaatacacc aaatttcttt tgttccgtat cttcttgttc tgcttcgatg aaaccaaaag
     2941 aatcattcga aaagacaaca ttaataattg gaagctgata tttgacttgt gtaatgatat
     3001 cttgcatgac catagcaaac ccgccatcac cacttaaggt aaatacttgt ttttctgggt
     3061 agcttagttg tgcggcaatt cctcctggca caccattacc catagttgcg aaccatccag
     3121 atgtcgtatg tttttgttta ccgttcatat tcaataaacg aatagaatgg atagtcgtgt
     3181 taccgacatc tgtgacgaaa atcgcatcgt cttcagcaat acgattgatt tctttataga
     3241 cagcctctgg acgaatcggt tcttcctctc gatcttcaaa agatttcaac caatttaccc
     3301 atttttcttt attttcttga tttgctaggt accagctatc agcagggcga gcatccccta
     3361 actcgacaag tctttgcaaa gctgtgtttg catcaccgag gatcgacaaa gatgtttcat
     3421 ggcgtcgtcc aaattttgac gcatcgatat cgatttggat aaattcagca gttggattaa
     3481 aaaatgcacg tccaaatggg aagtcgctac cgacaaataa gatcagatcg gcttctgcca
     3541 atgcttcatt tccgggttta gtagcgacac gtccagcaaa accaagaaag ttttcataat
     3601 catcaggaat aattcctttt gctagaacgg aagcgacaac aggaactgaa aaatgttctg
     3661 agaattcttt gatttgcgga aatccgtttc gcgttccttg accaacataa agaactggtt
     3721 tttttgcttt ttcaagataa ggcaaagcag ctagcaaatc tttttcttct ggtaatatca
     3781 cacttgtttt gtgagtgtga gcggtggaga aaggttgttc gtcaatttct tcaaaaccaa
     3841 gatcaacagg gatagtcacg atagccacgc ctttatgctc ataggcggct ttaatggctt
     3901 catctactac atgcggaaga ctttgtggtg tcatgactgt acgattgtat acgctaacat
     3961 ctgcgaaaat aggattttca tttagttcct ggaaagagtt atagttcatg gatgtagaag
     4021 caacttgtcc taagaacgct actagaggca catggtccat ctgtgcatca tacaaaccat
     4081 tgatcaaatg tgtcgcacca ggtcctgcag agccgaagac agcgccgact ttccccgtta
     4141 attttgcatc tgcagcggct gcgagtgcgc cgacttcttc atgacgaact tgaatgtagt
     4201 tgatttcggt tttttcttga tatagcgcat ccataattga attgaaagat cctccgggaa
     4261 tgccgtaaat atggtcgata ccccagcttt caagaacttt taccattgcg attcctgcat
     4321 tgatttttga catggtgttt cactccttta gcaatgatga tacataaatt ttttgtatct
     4381 agagtataga actttttatc gaaaatccca actaattcgt cttttcacaa aaatgtagtc
     4441 atgtttttgg gaatatgcta gagtagtaca tagatagatt caagagaggg gaatggcatg
     4501 gttgaatggc tattgagact tgaaccttgg cagcaagcac ttgctgggac agcttttact
     4561 tattttatga cagctcttgg tgccgggttg gtttttttct ttaaagaaat caaaaaagaa
     4621 gtgctaaact tgatgttagg atttgcttct ggtgtcatga ttgctgcaag tttttggtct
     4681 ttgttagatc cggctattac aaaagcagaa gaaaatggag atattgcttg gctagttgta
     4741 agtattgggt ttggtttagg cggtgtgttt ttatatatgg cagataaaac actgccccat
     4801 atgcattttg gaccacaaca tgaagcagaa ggattgccta cccatttaaa acggacgatc
     4861 ttgcttgttt tttctattac attgcataac attcctgaag gtttagccgt gggggtagca
     4921 ttcggtgcag cagcaactgc ggataatccg acagccgcta tattagccgc tgtttcggtg
     4981 gcgctaggta ttggtatcca aaacttccca gaaggagcag ctgtatcaat cccgttgcga
     5041 caagaaggtc tgagtcgtaa aaaggccttt gtttacggac aagcttctgg aatcgttgaa
     5101 ccaattgctg gagtgattgg agcgctacta gtaacgaaag tggagctgct gcttccttat
     5161 gcacttgctt ttgccgcagg agcaatgatt tatgtcgtgg tggaagaatt gatccctgaa
     5221 gcacagcaga cattgtccag caaacgtcat tttgcagttt ttggcgtcat gagtggattt
     5281 atcattatga tgattttgga tgtggcatta ggataaataa aaaaaatata tatgatatat
     5341 caaatagggc cgcgaactta tatcgcgacc ctattttttt agtaagttat atcataatga
     5401 agtgaatggt atcatacatt aataatagtt ttttctatgt gtatttcctt tgatccagcg
     5461 aacaagccag atgataagtg cgattgggat tagaactttg accgcaaacc ataaaagccc
     5521 gcttaggaca ctgacaaaaa gactgatgac gaagctagct gcaattagca ctaaaagaac
     5581 catgaagata tttaagaaca tatttttttg acgcatacaa aaccctcctc ttgttcattc
     5641 tttactatag aaaaagtata acagggattg tttaaaaaca cctccaactg aagactgatt
     5701 tttacttcat ctacttaaat gatcaaacaa ttgctttttc tctggtttat catctaaaat
     5761 agagcggaaa aggaggagaa tatcatgcct aaaaatcgtc ttgaagcatt tacagatgct
     5821 gtaatcgcga ttatcatgac tattttagtt ttagaacttc atgaacctaa cggagacacg
     5881 tgggaggcgc tcgctgcatt aggttaccga ttttttatct atattgtcag ttttatcatt
     5941 ttagcgattt attggaacaa tcatcatcat ttatttcaag ttgtacataa gataaatgga
     6001 cgggtcttat gggcgaacag tttctttgtt tttgcgcttt cgctttttcc gtttgcaact
     6061 tcatggatca gtgagtacat caatagcctg gctccagaaa tgacttatgg ttttgttacc
     6121 ttgaccgcta atattgcgta ttatatcttg gcaaaagaac ttgtcaaagc aaataaaggc
     6181 cacgagaaag cattgaaggc gatgttgaat aattacaaga aatcgtatct atcgattggt
     6241 ttgaatgtgt taggaattgt cttagggtat ttagtagcgc cagtagccgt gctgatcgca
     6301 aacatcgtga ttctagctgc ttggatcatc ccaagtcgta ccatcgaatc acaatatcgg
     6361 taaaatgatc cgtgtttaaa atttaagaga aaaatataga aaaaataaat aaacgatcaa
     6421 atatccgagt gatactagta ttcaaagagg gatgcagatg gatcaaaaaa gaattcatgt
     6481 aggactgatt actagttttg tcacgatgct tatttatttg gatcaaggtc atttgacttg
     6541 gacagtagtt ggtgtcgtag gatttttgac attattcaca gcaataaaaa aataaacatc
     6601 tttatgtaat ctgatgttct atctatttaa gaaaagcatg tttacagcag tatgattctg
     6661 ttgtaaacgt gtttttattt gtggaaaatt taatttttag aaaagtgtat cgaaaaatag
     6721 atagttagtt tttccacagt ggataacttc gtaagagata aaaataaaaa attagtcaaa
     6781 gaagggcgta tagatttctt ttgataggag atatacaaca gaaatgaata aatattaacg
     6841 tgttttcaaa cagtataaaa cggtgtggtt tttggaataa aaaaataaca tatctcattt
     6901 tacgcttttg ttattttata cttaaaaaat aacgacacag aaaataccgt gtctcagatt
     6961 tctctactac ttctaaaaac tactgttttt ttcctttgaa atgccttcaa aaagtaggat
     7021 caggcagaat atactggcag aatcaaggag aagggagcct aaataaggag aaaaaccata
     7081 gaaaatataa agtaattact tataaaacaa gctttttata gaaaaataat atacctgagt
     7141 agggtataaa ttaagacgga attcctttct agattgagat attgactagg aagaatgtta
     7201 agattttaag cctttaagct aaaaaaaaac cctttttgtt aaataagggg aatatagcct
     7261 gtcagtttca aactgcaagt cgtcctgctg caataatata gtagattaga gatgaaaata
     7321 gattttttta aaccaatgac tgaataaaaa gaccgtcata gatacacatt ggaatgtgtc
     7381 tattacggtc tttttttacg aacgatttta ttgaagacgg acagttcggg tcaaaaataa
     7441 aaaagctacg atcagaaata agaataaaat gtatcccaaa ataatggcta gacggtctaa
     7501 cttatttgtc gatagatgtt tgtttagaag ttgataggcc cataaaccta tcagtcctcc
     7561 tgttgtatta gttatcaaat cagtgatgtc cgctacgcca ataccgaata ggaattgaat
     7621 caattctgcc aagaagctta caaggaaaat aatccctaat tttttagaaa aagaaagtct
     7681 cttgaaattg acattcaaaa gcaccccaaa aggaaaaaag acaatcaagt tataagtggt
     7741 ctcacgtaat accgtcgtat tttcaaaaga aaaagtagaa aaagggacga gattgacatt
     7801 gctgtaagaa taagtgagaa attgtggaag attaaatgat agcttgaata aaatcaacca
     7861 taatagaaag aataaataaa tccctaataa aaattttgat acataccgca tagacatctc
     7921 ctccatttaa aaagtatacc atcgaaaacc gctgaaaaat cataaaaaag tatgaagttt
     7981 aaggaccaac gtagttatcg tacgaaaatt agaagctttt ttttgttggg aatccggttt
     8041 ctttaacaaa gctaagatag cgagttgctt ggaaaagttg ctatcttttt tctgtcaaaa
     8101 cgactcggaa tgttcgtttt ttgttctaat ttctccaagt tgtttacttt ttgatactaa
     8161 gtttatgaaa aatcaagaaa tagttgttat actaggtaat gctgacaaac gggattggca
     8221 tttcatcttg gttcagcttt ttttccaatt actaggaagg agggatagat tgttcaagac
     8281 ttattttcgt ttcaataaaa tacaagataa tccttttcgt atcatgaatc tgatgctact
     8341 tatctttatt gtattatggt taggttacat aaatcatgat tttttgattt caagtttttc
     8401 atcattaggg atctttactt ttttctatta tcaaaacata ccgatgaaat atttgctgaa
     8461 acgtatgttt ctgatcggtt gcggactgtt ggttgctttc gttttgggga tcttatcgac
     8521 ttatgtttta tggcttgaac catttgccgt agctttggtt gctttttcca gcagattcgt
     8581 gttgcgatta ttccatattt ctaaaccagg tggcttgttt tttgcgatgc tttctgccat
     8641 gggaacgagc atgcagctgc caatcgctca attaccgatc gtctcacttt atttttttat
     8701 gggtgtcgtt tttgccctga ttgcggcagt gattacaaaa ttactggatt cacggcctga
     8761 gcagacaatt gaaaaagcaa ctttaaaaga aagatttcac gaagagcctt tagtgatcat
     8821 tgattctgtt ttctacagcg ctgccttatt tctttccgtt tatgtcagtc acggcttgaa
     8881 tctgcataat ccttattggt tgaccttgtc atgtgcatcg attttgctag cagaaaactt
     8941 ggatgcgatg aagcatcgac aagtccaata tctgattgga tcgatgggtg ggttatgtgt
     9001 gtcggcattc ttgtcatttg tgccgtttac gcagttgcag accatttttc tgatcacttt
     9061 tctatatgga atcgcacaat ttctagttgc tagaaattat gcagttgcta atatttttct
     9121 taatccgatg gcgttgatgc tatctacact tattcgtgga gcatacttga tttctttgat
     9181 cgaatatcgt tttttaggga tcgtcatagg aagtttcatc ggtttaggtg ttgcgtgggt
     9241 aatgacggtt ggtttgcaac attatttaac ggttgttcgt caaaaattag aatagaagga
     9301 caaatatatg tgccaaaaga ggttatgaca agatttttgt cacaacctct aattcaaggt
     9361 gttcaaaagc cagattctca gcattaagtc aaattcacac aaaaatgtgg aaaggaactt
     9421 tcaaaaaaat tttatattta ttctatcaaa aaattttata tttattctat caaaaaattt
     9481 tgtttatgat ggtaactgaa taaactttcc tgagatggtc aatgctttcc ttgaaagtct
     9541 tacagatatt ctttcttcat agaaagttta agttttatag ttagtgtaca ataaaagtat
     9601 gtttatacca tatggtgtcc ggataatcag tcatttaaac aatgaggttc aaagatatga
     9661 aaaggggaaa tttgatgaag aaaataagca atgaaatcca aattctggca gaaactttaa
     9721 ctcttcccaa agtcgttata gagcaaatga ttgtacaagc aaaaaacatc cttgatgagg
     9781 tgtttgagca atatagtcat gaactaatgg atcctaataa agcaaaggcg actgcaaaga
     9841 aaatagacaa agtctatgaa gaggaacctt ttgcagcatt agctatttat ctatatgcag
     9901 cttctcaaag ttggaaaaaa atttatcaac cattgaagat tccaagaact atctatttgg
     9961 caacaatgaa tgcttttact agatttattc atgaacattt ccaagtaaca ggaagctatc
    10021 gctttgaccg tggtttttgg atttggcatt acactagtgg cttgatcttt cggattggtg
    10081 aattggagta cgaaatgaca tattttccga aagggtacac ggatctaaga ttagaaggaa
    10141 agcagtgcct ttctcttcat attccttccg atgcagattt atctcagaat aagataagag
    10201 atagttacga aagtgccatt cgtttttttg aactttattt tcctgattac cattaccaag
    10261 tgatgtacac agatacttgg ttactatcac ccaatcttac taaatggttg aaaaaagagt
    10321 caaaaatctg tctttttgca gccgattatc gattattatc agttgatgaa caggacgata
    10381 gtggagtccc ttggatcttt ggacgagtag atgcccaaat acatgattat ccagaaagta
    10441 cgagtttaca gcgacaagca aaagagcaat tgcttgcagg cgagcacatt ggctctggct
    10501 taggtattct tacttcataa gtttagtact tcgaaatagt ttaaaagagc gtgagacaaa
    10561 agtaaagaac acttttgtct cacgctctaa acacgtataa acggcggaag cagaagcaac
    10621 tccttcggaa ataagctgaa attcacaaaa atttgaaaag caattttcgt gaatttcttc
    10681 ttatttcttg gagttaaacg cttctgtccc agcctctttt ttagtgttct atcgaacaca
    10741 cagtacgttc aataggtaaa ctagctataa caatagatat taaagttaac ggacaagagg
    10801 gcctttttta ggtagctaaa acgtaataat gaaaatttat ttccagagat aggagtcaat
    10861 cagctagtct gatcgactgt tttaacaagt tgactttcat tttaactttt gataccattt
    10921 aaaacacaaa tcgtaattgt ttcgatttgt gttttttagc atgactacaa tcaaagaaga
    10981 aagtgggtcg tccatatgga aaaaacagat atctcaagtg cataccgccg cttgaaaagc
    11041 ccaaatatca aaacacgaaa acgtgcgctg aagatcatca aagatgtgaa acggaatagt
    11101 gggaaaaggt aagaagaagg aaacttaatt aaaaaaatag ccgaaaaacg tttttcggct
    11161 attttcaata atcggttttt gtgtaaggtt tccgaaagaa ctgctgttga ttttccattt
    11221 ttaacatatg ataaattggt agaatctttc ttttcaaatt gataccattc cctaattcca
    11281 gttatatagt taactacaga accacaaagc acgtacgttt ataaaagtat tatctgtagt
    11341 tatttttcaa ttccctttca acctcttatt ttgaccagta gtattgatca ctttaccttt
    11401 taacaccttt gttcgttttt gaaacatttt tagtcaagaa tggaaacgta actacataat
    11461 caaaggtttt gcgaaatatt tcatgaaata tttcatgtag aagcgagcaa tacggttgca
    11521 aataaccatt atcgtttaat aagcagttac gaaagctccc aatatctcta acgttcagct
    11581 cactccattt cttttcttca aaaaaatcag tgattcgtat ctctttctat ttgtcgaaaa
    11641 agttaacttt aaaaagtaac gccgaaaaaa tatgcaaaaa aataaaaaag cagttactat
    11701 tgctagaata acatcaagga ataataagtt ttggttttca gataatagtc gataagttgt
    11761 gtattggaat tttagataca gtaataaagt atttttcatg aaattttgtg agaatcacta
    11821 ttacaagtat ctttaataaa attaatggtt aaggaggaac ttaaagtaga atgtaaatga
    11881 atagttgtag tcagtcgatt gtgatactag actcaaagtt gcaggaataa gtttttcaat
    11941 ttgtgattgg aagataaaaa atgaaaaaga agggcttttt tttatacttt gttgattgta
    12001 gtgtttattt tccctctgtt tagccaagta acattttcta tgactcaacc agtttcaagt
    12061 gaggaacatg ataacctttt tcgtgctaca gaagagttta agaaatatca ggaaaatatt
    12121 gttccggaga ctccattagc tagtgagcca attactgata ttgatggaaa ccaaatagga
    12181 tggtatatac agtacgaagt tatggtggta aataatgact caatagtaaa aaatacagta
    12241 ggcttagagc taacaccgat tgaaaaggtg agttcattgt taacgtatat ttatactgat
    12301 gaaagtgacg agactgagac cattttaacg gattactcaa aaattgctac tgataaaaag
    12361 tatatattgt ggatgtagac actagtgaaa tactttatga tatagaaatt gatgaaaatt
    12421 tactagatgt agcacaagaa atcgatcatc aaaagcagga agctattcga tatgctggta
    12481 gaaaagggaa aagaaaatat ttattacgtg aatgtagcaa aagttagaga agatgaaaat
    12541 gagtggaaag aattcaagag tcgctactca attaactcga ctccgacatt tacagtttac
    12601 agagaaggat cgatagaaaa aactgttttt tggactaaag aaagtggaat gtctttagct
    12661 gaagtagaag agtttttgga ttatgttagt atgcaacagt aagggagttc ttattatgaa
    12721 aaattttgac acatattatg ttattgtttt ttcgatatct ttgctgttaa tattactaac
    12781 tttaatgcag tataaagagt atccagagta ttttatttta acttttgtat atttagtaag
    12841 tagctacaca cttctatata aattcagaag ttcttctaga gtttctaaag tttgtttctt
    12901 aattagctta ttgattctat aatatttttg atttatatag gaaattaaaa atattataga
    12961 atcaacactc agagaacggg ttggttttta atagatttaa taagtactga taatgaaatt
    13021 tctcctggtt gggaaaacga agttggttac cctgtagaag aattttttca agattacttt
    13081 gcggagtatg ccgataggat aatgagagat tacgtaacag ataaaactca agaagtcatc
    13141 aatcaatccc agtcattaaa tgaattatct gataaggcga aagaagaagc agttgccatt
    13201 atttcttcag ggattagtga ctatattgat atgagtggtg aaatcaatta tggcgataaa
    13261 gacaaaattg tacgcatgat aaaagctttt atcaaaactt ttgatgtgaa ggcagatctt
    13321 aagtttaatg atacttacct agtaggaaaa ttaattaata tccttgggac agagaaagat
    13381 acggaaacgt ttatttcctc tttgtctatg ttgtttacag ataagcattt ttcaactcag
    13441 cttggtggag aggaactagt ggaaatcttt taaccattac gcccagcatt aataaaagta
    13501 tatcaaaaaa gtactcctga tgatttttaa gattggtttg agaaataatg tgaaaacact
    13561 atttttaatg ttaattatag atagagatga acgcgtcata tggttaaaag aatgaaaaag
    13621 tcaaagatac actgctatat ttcactaagg atatagcagt gtatctttca tgtcgtttca
    13681 ctggcagttt tttataaaag gaggtgtgca tttcagttgc ttttctttgg tagttagttg
    13741 aatcgtttaa gaattaacgg atgtggcaga caggtgctct gtccaaaaac cagtaaaatc
    13801 tttgatggtt ccatctttat ttagctgtgc ctgttcaaat actgttttgt ttttaccgaa
    13861 tggatcaatt gcaataattg ttccgatccc cgcacgtttg gcagcaagta atcctgagta
    13921 agcatcttcg ataacaagac aatcagccgg agctaagctc agtttgtctg ccgctttaag
    13981 aaaaatatcc ggttgcggtt tcccagggaa agagccgtcg tcatagacaa tcttgtccca
    14041 gtcaaaccag cgggctaaat cgaaaatatc aaaataaaaa gcaacattct ctttgacagt
    14101 agccgtggca atcgttaatg gaatctgttg gtcagtcagc tggtcaagag tcgcttcaag
    14161 cccttttgtc aactgaagct cgttttcatt ttgcaagcaa agcatacgat aatagctttc
    14221 tttttcttct gacaatacgg cgatctcaga ggcggtcaaa tgctctgaaa tgaaatgttt
    14281 caaaatctcc gtattcgtcc gaccatgaat attttttaaa atatcctcgt ctgataaacc
    14341 atcttttgta tgatcatgta tcatatgcag ccaagcagct tcatgcagat gagagtccaa
    14401 aaacattgtt ccgttgaaat caaatataat ccctttcata gttacgctcc tatagtggta
    14461 ttttcggttg aatcagcaat ttgcagatcg acatattgtg catagtgctc ttgaacctcc
    14521 cgcgagatct ggcgatccgt aatgagcaga tcgaaatcat gcaaattata aaacacataa
    14581 aaatcatctc gattgaattt tttattatca acgagtagat atttcttacg ggaattattt
    14641 agagcgatct gctgtgcttc tccttcttcg gtactgtaag tagagatcgc ttcgttgtga
    14701 acgccatttg aactgataaa tgcttttgta aactttagtc ttcttaaatt gtcgttggtg
    14761 atcggtccga caaatgcgcc ggtatttttc cggaaatcac cgccaatcag tagaatttct
    14821 ggtccatctg cttgagcaag aatatcaaaa actggataat tatttgtaat gatccgaatt
    14881 ttcttgttca gtaaatgcgt cgccaatagt tcgattgttg tacctggtcc taagaaaatc
    14941 gtttctccct cgtgaatcaa ggaggctgct gtggcagcaa tttctttttt ttcttgaatc
    15001 tgcagagtgg ctttttcagt gtgagaaagt tcttgatcta aagtgtagga aagactttga
    15061 gcccctccat ggacacgcag cagcttgccc gatttttcta attcatctaa atctctgcga
    15121 acggtcatat cagaaacatc taattgattg atgatttcat taacggtaag gattcctttt
    15181 ttattgacca tttctacgat agtaagcaat ctttcttttt tcaacattgt cttcacctct
    15241 aatttaagga taatctatca tataaacgga caacagtcaa tctaattcga acaaaagtaa
    15301 acatttaggg tggtttttat gtttgttaca atagatttta ttgctgtttt ctggttttta
    15361 tgattatttg tgtttgtttt tgttgacaaa tgttttggat gcgtttacaa tgcaatcagt
    15421 tagaaagagc gaaaactgat tatagatttg aggagagatg tacatgctta aattaccaga
    15481 agattttatt tttggcggag ccacagcagc ctatcaggtt gaaggagcca ctaaggaagg
    15541 tggcaaaggc gctgttgctt gggatgactt tttagaggaa caagggcgat tcagtcccga
    15601 tccggcaagt gatttttatc atcaatatgc aaaggacatt gaattgtgtg aacgttttgg
    15661 tgtaaatggt ctaagattat ccattgcttg gagccgcatt tttcctgatg gagctgggaa
    15721 accaaatcca gaaggaatcg ctttttatca tcgtgtgttc gaagaatgta agaaaagaaa
    15781 cgtaacgccg tttgtgacgt tgcatcattt tgatacccct aaacgtttgt ttgaccatgg
    15841 agattttttg aatcgagaaa caatagaagc atttgtttcc tatgccattt tttgttttca
    15901 tgaattcaaa gaagtcaagg tgtggagtac ctttaatgaa atctatccag ttgcaaccaa
    15961 tcaatatttg ttaggtgttt tcccaccagg aatcaaatat gattttacaa agatcgtcgc
    16021 ctgtcttcat aatatgatgg ttgctcatgc ccgtgtagtg aactatttca aagaaaacga
    16081 acttccgggt gaaattggtg tagttcactc attagagacg aaatatgcgg caacagacgc
    16141 gccggaagat aagcatgctg cctttttaga cgatgcattg tcgatccgtt tcttattaga
    16201 tgctacctat ttaggctatt attcaactga aaccttgact gctcttgatg agatatgtga
    16261 agcaaatcag gccagctatc attttcctga agaagatttc gttgaattga aaaaagcatc
    16321 cacgcgtaat gactacttag ggatcaatca ctaccaatgc cattttgtca aagcttacga
    16381 tggagaaaat gcaatccatc ataacggtac gggtgaaaaa gggacctctg tttataaagt
    16441 gaagggaatc ggagaacgta tttataaaga aggaattcct cgaactgatt gggactggct
    16501 gatttatcca gaaggtctat atgacttgtt gctgcgaatt aaaagcgatt acccacatta
    16561 caacaaaatc tatatcactg agaacgggat ggggtataaa gatcagtttg aagatggcat
    16621 tattatggat cagccgagaa tcgactattt gagagtctat ttggaatcct taagcaaggc
    16681 gataacagcc ggggtaaatg tgaaggggta ttttttgtgg tcgcttatgg atcttttctc
    16741 atggacaaat gggtataaca aaaggtatgg cttgttttat gttgattttg aaacccagaa
    16801 gcgttaccca aaagaatcag cttactggta caagttggtc agtgaaacca aaacgattat
    16861 ttaataagtg ataatggagg aagaaaagat gaataaagag gaattacaaa tgcttggttt
    16921 tgagatcgtt gcatattcag gcgacgctcg cagtacatta ttgaaattat tgaaagaagt
    16981 aagacaagga aactttgaac atgttgattc ggcgttgaaa gaagccgacg aaaatttaac
    17041 ccttgcacat aattcgcaaa caaaaatttt agcggaagaa gcatctggga aagagatgga
    17101 gatgggattc atttttatcc atgggcagga tcatttaatg accactttat tgctgagaga
    17161 tctgattcaa gactttattg tactgtacag acagaatcaa ggataggaga agaacgctat
    17221 ggatggaatc attaaacaaa tcgaaaaagg gaaaccattt tttgaaaaaa tctcacgtaa
    17281 tagttattta ggagccgttc gtgacggatt tttaacagcg atgccagcta ttttattttc
    17341 aagcattttc attttgattg ctgcggttcc tgagattttt ggttaccaat ggcctgaatc
    17401 tgtctccaca tggctatgga aagtctataa ttattcgatg ggtgttgtag ggttactcgt
    17461 ttcagcaacc actgctcgct gcttggctga ttcgatgaat cgtcgcttac cgaaaaacaa
    17521 agtaatcaac accacctctg tgatgctggc ctctgtggta agtttcctgt tgttaagcgt
    17581 ctctccgatt gaaggtggat tttcaacgga atatatgggg acaaagggac tactggcttc
    17641 ctttgtcgct gcatttttaa cagttaattt atataagttt tgtgtgctga aagatattac
    17701 cattaaaatg cctaaagaag ttcctggaac gatttcgcaa accttccgag atatttttcc
    17761 tttctctttt tcagtatttg cggccgtttt aattgattta ggcattcgac aaatcttcca
    17821 aatctcattt gcagaagcaa tcattacggt gctgcagccg ctatttacag cagctgacgg
    17881 gtacttagga atcgctatta tctggggagc gatggcgctg ttttggtttg tcggtgttca
    17941 tggaccgtcg atcgtggagc cggcaattgc ggcaattatc tatgccaatg tggaaaccaa
    18001 tctgcaattg ttcaattccg gtgaacaagc ttctaaagta ctgacggtgg gtttagggaa
    18061 ttttgtagga actatgggag gaactggggc aacattggtt gttcctttta tcttcctact
    18121 ctttgctaag tctaagcagt tgaaggcagt tgggaaagct tcctttattc cagtaagttt
    18181 cgctgtcaac gaaccattgc tatttgcggc acccatgatt ttgaaccctt atttttttat
    18241 tccatttttg ttaacacctg ttgtaaacgt ttgcctgttc aagttttttg tcgatgtatt
    18301 gggcatgaac agctttatgt acgttttgcc ttgggcaact ccagcaccga tcggtttggt
    18361 tctaggaacg ggcatgggta tcttagcttt tgttctagca gcggttctaa tcgtcgttga
    18421 cttcctgatc tatctaccat tttgcaaagc atacgatgct gtcttagtag agaaagaagc
    18481 acaaaaagca gaggtgatcg caaccgagga aggtgcttct gccgatgtac gtgaggaagt
    18541 acctgtggca gcgagtcaag ttatcagcga tgcaaataaa gaaatcaatg tgttggtact
    18601 ttgtgctggc gcagggacaa gtgcgatgtt agctaatgct ttaaaaaaag gtgcatctga
    18661 atcgaaagca ccgatcagtg caatggcggg tgcttatggt tctcattacg aaatgctgca
    18721 ggattttgat atggtggtct tagcaccgca agtcaattct tactatgatg atatcaaaga
    18781 agacaccgat cgcttaggaa tcaagctggt tgcgacaaaa ggagcagaat atatcggatt
    18841 aacgagagat cctgaaaaag ctgttcagtt tattctgaac caatttcaat aaaggaggag
    18901 ccataatgaa aatgggaatt tggttgcgta aagctgtttt atttgcagct gtgttagggg
    18961 ctttgggggc tgtttcaaaa actgttgaag catctgccag cgaatttatt cgcggagcag
    19021 atatttcgat cctgtcagat atggaaaaat ctggtgcaaa atattatgaa gacgatattt
    19081 caaaagatgc cttagagatt ttaaaaaata atggtgtcaa ctatgttcga ctgcgtttgt
    19141 ggcaggatcc ttataatagt aatggagaaa gttatggagc agggacaaat gatctggaaa
    19201 cgacgattga ccttgcaaaa cgagcaaaaa atctgggact taaggtgctg ctggattttc
    19261 actacagtga tttttgggtt gatcctggga aacagaatct tcctaaagca tggcaaggtc
    19321 tgacctttga agaaatgaat acggcattgt acgactatac aaagaacgtt ctgtcggaaa
    19381 tgaagcagct ggacgtttat cctgatatgg ttcagatcgg caatgaactg aatagcggaa
    19441 tgctctggcc ttatggcaag tcttggggag aaggcggggg agaatttgat cgattggctg
    19501 cttttttgaa gtctggtatc caagctgttc gagacactca acctaaaaca actccagtaa
    19561 tgctccactt agctgatggt ggcgatacag gagcatttac ttggtggttt gatgaaatca
    19621 cgagtcgagg tgtgtcattt gatttaattg gagtgtcgta ttatccgtat tggcacggtt
    19681 caatgaatga acttcaaacc aatatgaatg cgatcagcca acggtatggt aaagaagtga
    19741 tcgtagttga aacagcttat gggcatacaa cagcgaatgc cgatactatg ccaaatgctt
    19801 ttggtgaagc cgaagcagct gcggggggat atgaaccaac accgacagga caagcagagt
    19861 atttgctgga tcttgccgat cggattcagg cagtaccgaa taatcgcggg gcgggcttct
    19921 tctattggga acctctttgg tacaacggaa atgtgagttg ggcaacacaa gcaggtatga
    19981 attatctcag tgttcaatcc actatgggaa acgaatggga caaccaagcg atgtttgatt
    20041 ttgctggaaa cgccttgcca agcttgcgtg cgtttaaaca agcaggggcg caaataaatc
    20101 tagttaaaaa tgcaagtttc gaaagcgatg gaccaacgac cagtccttcc aattggcaaa
    20161 cctggtttca acaaggaacc gctaagggta cgatcaaaac ggaagcgggc actttttttg
    20221 gggactacaa actaactttt tggcaggatc aagcatacga ggcatcagct tatcaaaatg
    20281 ttagtggcat aagcaaagga acttacacat tgagtgccta tgtaatgtct gggggagagt
    20341 ttgatactaa tcagttatat atcaaaaatt atggtggagc ggaactaaat actgcggttg
    20401 ttaaaacaag tgaatggaca aaaattacaa tcaaagacat tccaattaca acaggtgttt
    20461 gtgaaattgg ggtttatagc aaggcttcag gaaatgcttg gacgaacgtt gattttgttt
    20521 cattggtgaa acaatagact gcggatcgtt tcgattcaag atcggaggtt cccttcagcc
    20581 acatcgttag cgtagaaggc aggaatcgaa cctgcacctc gcctttgcca aaaataacca
    20641 gaaacgattt ccagtcattt ttaccaaaac gagtaaagca ccagacttca tctacacttt
    20701 gtattatgct aaagagagtt caacgatttc gtaagaagca atgaaaaaat tgcaaaaagt
    20761 tgtgcagata acataaagtt tgtttaagaa agggtaacct tagaattccg tcgtgaatat
    20821 gatttgagca cttagatgga tagaaaaaag ttctcggaaa acagttcgtt tttcgagaac
    20881 ttctttttat gataagaatg aaacaaatta acttatatta tagtctcttt tggtagggaa
    20941 ccatcttatt tttaatgaaa atcaagcatt ttagctattg aagaatgttg atttataggg
    21001 attccccctc catattatgt tggctcttta ttcccactct gttttcaacg taaagtgtat
    21061 aaaacagaat gcgatactat caagggagtt aaaacaatat acagaacagt aaaacactat
    21121 aaataaaaat aaaagtgcaa taaaagtgca atttttgcac ctaatcatac tctgtttata
    21181 tatgaaaact agattctgaa tccaatattt ggttaacgag aggcgttcga atttgaacgt
    21241 cttttatata ttactttcaa ataaatatga ggtagaatac tatctatttt ctttttaacc
    21301 caattgttat aatggagagt gtaaaatatt ttgtgtaaat agaaaaaagg aagtcccttc
    21361 tgtagaatag agttaccaca acacattcac agaagagagg acttccctat gaacgatttt
    21421 actacagaaa ttctaaagac tctagcgaac aaaggcgatt tgaatgaatt attccgtgtc
    21481 catttggaga aagcagtcaa tacgcttctc aaaacggagt taacggcttt cctagattac
    21541 gaaaagtatg atcgcattgg ttttaacacg ggtaattctc gtaacggctc ctatgaccgt
    21601 acggtcaaga ccgagtatgg ggaacttcat ctccagattc cgcgcgaccg caatggtgag
    21661 ttcaagcaac agactgttcc tgcttataga cggacgaatg acacgttaga ggagaccgtc
    21721 attcacctct tccgaaaagg tattaccatg tcggaaatcg cagacttgat tgagaaaatg
    21781 tatgggcatt actacacgcc ccaaaccatg tccaatataa caaaatcatt tacagaagag
    21841 gtaacggcgt ttaatgggcg ggagcttcat gaccgttatg ctgctattta tatggacgca
    21901 acgtatattc cgttaaagcg gaaaaccgtc gccaaggaag ctattcatat cgcagttggc
    21961 attcgcccgg acggatcaaa ggaagtattg agctatgcga ttgcaccgac tgaatccatc
    22021 acgatttggg aggaaatttt attggacctt caagagcgcg gtttgaaaaa tgtcctcctg
    22081 ttcatcacgg atggcttaaa ggggatggta ggagcgatta gtcggttcta tcccaaagct
    22141 cgttttcaac attgttgtgt acacgtttcc cgtaatatca gtcataaagt gcgtgtcgat
    22201 gatcgtaagg aagtctgtga tgattttaaa atggtgtatc aagcctcatc taaagaggtg
    22261 gcgttggaag cacgtggtac ttttgcggaa aaatggaaaa ccagctatcc aaaagtggtt
    22321 gaatcgattc tttcgaacga tcacttgctc actttctatg atttcccctt ggccatacgc
    22381 aagagtattt attctacgaa cttgattgaa tcctttaata aacaaatcaa gaaatacagc
    22441 caccgcaagg aacagttcca aaatgaagag tcgatggaac gtttcttagt ctcgtctttt
    22501 gatacttaca accaaaaatt cctaggtcgc agtcataaag gtttccaaca ggccgaaggc
    22561 gagctcgaac aaatgctaag ccaactgatt gagaattaga aacaatctgc agtagggaag
    22621 aatcatttac acaaaattat tgacgctccc ttataatgag aacttttact aacataattt
    22681 atgataaact tactatataa tcatattaat taaatagtta ggagatctgc ttatgtttat
    22741 atctactctt caaattcgaa attttagaaa ttatgaatcc gaaatctttc gattcgataa
    22801 tgagacaaat actattatag gtgagaatga ttcagggaaa accaatgctc ttactgcatt
    22861 gagaatctta ttagatgata gttattacta tagtagtaaa acactaaaag aatcagattt
    22921 ttttcatgga attcaaaatg ggtggcaagg gcattggatt attatctctg cgacatttga
    22981 aggaatcagc gaagaagaat ttgacaatga gatttgtgct tccatttctt taaattctga
    23041 aagtcaaaca atcttagaag aattgatttc aaatgcagat aaaggtgtag gtagtatttc
    23101 tctatttatt agacctaaca aagctattag aaagcaatta tttgatattt ccactgaatt
    23161 agactctcat caatttaacg aatttagaaa ttctattcgt ctatccgact atgaatttta
    23221 ctacacatct aaatcaaatc taaatttctg tatcaatgaa aattatgata aattggtagg
    23281 aagactcaat gacaatatag ctagtaatcc tgaagaagac gatgaagctc tgttgggctc
    23341 aagaattgac atgtcagata ttttcaagca tatttcggtt gtttatgttg acgctcttcg
    23401 agatgttctt agagaaatga aaaacaatcg aaacccagtc aaaagaatca tggaaacaat
    23461 tgaatcaaaa atatcatcag ataatgttga gtcattaaaa acaattatac agcaattaaa
    23521 tgagactatt accagtgttc ctgaaattag gaaaattggc gaaaatatta atcggcaact
    23581 aaatagcata attggttcag tgtattctcc taatcttctt ctttcctcaa ctatgtcaga
    23641 tgatatgggt tctttagcaa aatttctttc aatgaagcca gaacaaaata tcgacttaga
    23701 cttactaggg ttaggacatc taaatatgat ttatttagct ttgaaaatag tcgaatttga
    23761 agcgtgtcgt tcaagagaac tacttaatat tatgttgatt gaagaaccag aagctcatat
    23821 tcatcatcat attcaaaaaa ctctgttcga aggattaaat cttcaaaaaa actatactca
    23881 gattctcatg accacacatt ctgtacattt agctgaagtg tctgaaatat ctcggatgaa
    23941 tgtattaaaa tcgtttaatg gcaaatctat cgttatgaat ccagcaaata atctgaactc
    24001 atttgctaaa gaaaagttaa aaaagaacaa tctaaatcta ataaacgcag tagagagata
    24061 tttagatgtt aaaagaaatg gattgctttt ttctaaaggt gttatcttag ttgaaggaga
    24121 tgctgaagaa atacttattc ctcaaatagt aaaaaaatta tttggtataa acttagacga
    24181 acttggaata ggtttgatta atgttggtag tacttcgttt gaatatatcg ctagcttgtt
    24241 tgacgatgat agaatacaga gaaagtgtgc tattgtaact gatttagatg aacaggcaat
    24301 cccatcagaa catcgattgt ataaaagcac tgcacaagag aaaggtcaag caagaaaaga
    24361 aaaattatcc tcccttttta atgaaaacaa ttgggttaat tgtttttatg ctaatacaac
    24421 atttgaaatt gagtttttag atgtgaataa agaaaatatt ctaacttatg tcagtccaat
    24481 tatagatgac gcattcaaag ataaaaaaac aataactgct tataaagaag aaatacaaag
    24541 tgaacaatat ttagaaagaa atttagcgat gttaaaacta gccaatcatg ttggtaaggg
    24601 gtggtttgca attgagttag gtgaaaaaat tgacttttta atcaaaattc ctaagtatat
    24661 tcttaatgct attgcttttg catgtcagga aatagtagat ctctcaattt ataaaaaaat
    24721 tctactccat acaatcgact tgtacgagga aactacagaa ttgtcagctt tatcagctag
    24781 tttaaaaaca agtgatacag ctgatttgaa tatagcaata atttctttat tagagtactt
    24841 tggtaaagat gaggatgttg tgtatctaat caatcagata gaaaattaca caaaaatact
    24901 ttatgctgag gtttcaaaat gacaaatatc ttcgaaaaaa tcgtaaatga tttaaattta
    24961 gaacaaaaag atgttgtgga aagtgaagat aacctttatg taaatgcatg tcctgggagt
    25021 gggaaaacaa gggtacttac aaggaaaatt gcttaccaag caattaaaca cgatggatca
    25081 ctaaaaagaa taattgctat cacatacact aatagagctg ctgaagaaat taaggaacgc
    25141 ctaagtttac ttagtataga tgatgatatt aatatttggg tggggacaat acatcagttt
    25201 tgtcttgaat ttattattta tccttttaaa atgaatctct caagagtatc aaaaggattt
    25261 acaattattg atgattatac tcaaagagct tacgtcagcc aaattaataa tatgctgaat
    25321 ttaaatctca aaacgtatga actgaacaaa attgatttgt cactttctca tgattttaaa
    25381 attaatgaag ttcagtatac tcaagtttct gaaatgtatc acactatgtt ggaaaaaaat
    25441 aaagaaattg attttgatat gattttacta gtagcgtata aaattttaac tgtaaacagt
    25501 aacgttgcac gtaatattgc tagtaatata agggcgattt atgtggatga atttcaagat
    25561 actagagaat tacaatacaa tgtaatagct aaattacttc aaaacaaccc tcaaattcag
    25621 tcaatgtttg ttggagatat tgaccaagca atttatggat cattagatgg tatagctaaa
    25681 tcagttgaag aactagaaat gctaactagt cacacttttc aatcaaagac gctacatggc
    25741 tgttacaggt ctaatcagag attagtagac ttctactcta attttcagag ttgtccttac
    25801 gagattgaaa gcagagggaa taataaaaac gaccgtggtt ttataagtta tgattgcaaa
    25861 gttactaagg aggatttacc caacataata aaaaatataa taaaggaaaa gattgaaagc
    25921 ggcattcatg aaaaagatat ttgtgtactt gctcctcaac actatcctct gttttcactg
    25981 ggtgagtatt taaagaaaga attaccgtat tgcaattttg atgctataaa tatatcacca
    26041 attaaggtaa ataatcatag tttatttttc aagttagcaa ttttgtactt tactgaatca
    26101 ggtgaaaagg ttcgaagaag aaagctaatt gcaaacgata ttttaattat attaaaaaat
    26161 gaatttggaa tagaagttaa agattatttt attgacttag atttattaca gctaataaac
    26221 agtgtgaaaa gaatgtatag tggagacgac aacggtgttg atttatacaa atttgttacc
    26281 aaacatatat ttgttaatct taatattgac gagaaaaatt acccgtctat ttttaagcac
    26341 tatctatttt ttattaacga agtagaagat cggataaaaa ataattcatt atctagtagt
    26401 gtatctagtt ttaagaagat gtttaaatct cgagatggta tcacacttac tacagcacat
    26461 aaagttaaag gaaatgagta tgatactgtt atcgctatta atcttctaaa cgggaaaatt
    26521 cctcattgga atgaaatttt taatgtttct gataaaggaa ttagttcagc gagaaaacta
    26581 ctatacgtaa tttgttcaag agcaaaaaat aatttatttc ttttctcaga ggtgggttat
    26641 tttacgagta aaggagactt gctttatccc actcaattat tagataaaat agaatttgat
    26701 tataatattt aaaataccta ggtagtcgtt gttttaaata tagtaggaat cggacgctca
    26761 gtacggagcg tctatttttt tacccaattt tgaaaggaag tgaacttatg aaaataaaaa
    26821 atcaagaatc aaaaggtcgc tctcccccct ttaaaaccat cacacaaata agattcattc
    26881 aaacataaaa acagaaagga taggtaaaaa tatggaactt aaatatgtca ttcccaatat
    26941 ggaaaaaaca tttggacaat tagaatatgc tggtgaaggg aacattgaac aacgtcgaat
    27001 aaatggcaga ccaaccattc tctctcgtag ttacaattta tactcaacga ttcaacgagc
    27061 agatgatatt gttgtcattc ttccctccga agctggcgag aaacattttg aatccgacga
    27121 aaaagtcaaa ctcatcaacc cacatattac cgcagaaggt tataaaattg gcaatcgagg
    27181 atttaccaat tacattatgc acgcagacga tatggtgaaa gcataagaaa ggaaaggtga
    27241 aaaaatatga gattagcaga aggtattgtg attgaaaaag accagacatt tggcacattg
    27301 aaattttccg ccttgcgtcg tgaggttcgt atccagaatg aagatggtac ggtttccgaa
    27361 gaaattaaag aacgtaccta tgacttaaaa tcaaaaggac aaggacgcat gatacaagtc
    27421 agtatccccg cttctgttcc tttgaaagaa tttgactaca acgcagaagt ggaattaatc
    27481 aatcctgtgg cagataccgt agcgacggct acttttcgag gggcagacgt tgattggtac
    27541 gtgaaagcag acgatcttgt attaaaagga cagacaaaac ctcatcagaa atataaaaaa
    27601 gaagcgtccg aaagtgaaaa ataggtgttg cttatgacaa acacaccatt gattttgaaa
    27661 gaaattccga aggatgaagc catttctttt attcggcaat accattattc aaagatactc
    27721 cctagattgt gtaaatactt tctagggatt ttttcagaag aaaaattatt aggcgttgtg
    27781 gaattaggct ggggtacgca acccctacaa accatacgca aactctttcc cgacagttcc
    27841 cttcaaacca cagattattt agaaattgga aagatgtgtt ttcttccaga aatgaatcaa
    27901 accaattatt ttggcagtca agctctttca gctctaatta agtggttgaa agaacatacg
    27961 gactgtcatt ttttatatac actggcagat ggaattgagg gaaaatgtgg ctatgtctat
    28021 caagcgtcca acttctttta ctgcgggtat ttcaagacaa gtgtctatcg tgataagcag
    28081 tcatgggaaa agatacaccc tcgaagtgct cgccttttat tagaggaaaa tgctcgcttt
    28141 gaacaggtag agaaaaagca ttggctttcc caggcatttt gcgaatacaa agggattgag
    28201 aaaatcaatg gacgcatgtt tcgctatctc tatccgttga caaaagaagc gaaaaaactg
    28261 ttaggacaca ctctctatcg acgtcattat tatccgaaag aaaaaaattt acgctttgaa
    28321 aagcgaatcg cttatcagaa atatgaagcc atttctcaac caacttttga taaacaggct
    28381 cgtatctaca atacacaatt attttgatag gtggaaagga ggatttttat gccattgctt
    28441 catgacagag gagagcgaat caaagccaac gataagcact tggtctatca ttttgtacta
    28501 atttcattgc tggtagtttt tctagtcgtc ctactccctt ttcattggag agcgttacaa
    28561 tcggtgaact ggcaagcagt tcagttagat acatttttct atcaacttca tacgccatac
    28621 ttttggagca gtattctcac tgctcttttt gtatgcggac ttatcagctg gctttattat
    28681 cactatcgta tagattctat aaaacagtta gagcaccgtc aaaagttagc tcgtatgatt
    28741 ttagaaaatc attggtatga agccgaagaa actcaatcag agggattctt taaagattta
    28801 cccagcaaga aaaccaaaca acgaatccgc cattttccta aaatatatta tcgaatgaaa
    28861 gatggactaa tccatattca tgtagaaatc accttaggga aatatcaaga tcagcttttg
    28921 cacctagaaa acaaactaga aacaggattg tattgtgaat tagtggaaaa aacgttgaaa
    28981 gattcttatg tagagtatgt cctactttat gatactattg gcaatcgaat tggcattgaa
    29041 gacgtggtag ctcaagatgg acgtgtcaaa ttgatggaat cggtctattg ggagttcgat
    29101 tctctccctc acatgttaat tgctgggggc actggtagtg gaaaatccta ttttattctc
    29161 acattgattg aatctctcct gcatacaaat gcaaagcttt atattcttga ccctaaaaat
    29221 gctgatttgg cagacttagg ggcggtcatg aataatgtct attaccgtaa agaagatatg
    29281 ttggcatgta tcaatcaatt ttacgaagat atgattgatc gcagtgaaac catgaaacaa
    29341 caccctaact ataaaacagg agaaaattat gcttatcttg gtttgtctgc caattttctt
    29401 atttttgatg aatatgtggc aatgatggat atgctaggtc gtgaaagtac caccgttatt
    29461 cataaactca aacaaattgt aatgttagga cgacaagcag gcttcttttt aattcttgcc
    29521 tgtcaacgtc cagacgcaaa atattttagt gatgggattc gggacaactt taacttccgt
    29581 gtggctttgg gtcgtatgtc tgaaatgggc tttggtatga tgttcggaag cgatacacaa
    29641 aaacaatttt tcttaaaacc catcaaggga agaggttacg tggatacagg aaaaagtgtg
    29701 attagtgagt tttatacacc ccttgtacca aaaaggtacg atttcttagg agaaatagga
    29761 aaagtcattc aaaaaaagca atctgagcct gtaccgcacg aagtgaaagg tacaggttca
    29821 gacttgcttt gattggtgtg gcgttagcca cgccaatcct ttagccccct ctatctaaca
    29881 ggggggcaca aatacaactg aaaacaggca attctcatca accaatgttg atggtacaaa
    29941 ggattgcgga aaatatgaaa ggtgtaaact caagggtcat agtttacatc ttttttagat
    30001 tggaggtttg cgaatggaaa acgaaaaaat ttggattaag gaattgaaag aaaagcgact
    30061 ggcttatggc gtttctcaaa ataaactggc ggttgcttct catattacca gaccttacct
    30121 atcggatatt gaaacaggca aggcagtccc tactcaacag gtgaaagaag atttactgaa
    30181 cgctttggaa cgcttcaatc ccgataaccc attggagatg ttattcgact atgtacggat
    30241 tcgattccct accaatgatg tggctcaaat tatcggtgag gttttacgtt tgaatatgga
    30301 ttatatgcta cacgaagact ttggctatta ttcttatccc gaacactatc gtttcggtga
    30361 tattgttgtc ctagtctctc atgatgtaag taaaggtgtt ttattagaac tgaaaggaaa
    30421 aggctgtcgg caatttgaaa actttctatt ggctcaacat cggagctggt atgatttctt
    30481 tcaagattgc atggaacata aaggtatttt caaacgcttg gatttagcta tcaacgataa
    30541 aacagggatt ttgaatattc cagatttggc aaagaaatgt aaacaggaag aatgcatttc
    30601 cgtctttcga agctttaaaa attatcgcag tggtgaattg gttcaccgtg atgaaaaacc
    30661 atacatggga aacacacttt atattggctc attaaaaagt gaagtctatt tctgtatata
    30721 tgagaaagat tatgagcaat acgtgaaaaa tgatgttccc ttagaagacg cagaagtaaa
    30781 gaatcggttt gaaattcggt taaaaaatga ccgagccacc caagccatga aagatttatt
    30841 ggcacatcaa caggcggaaa aaacagcctt tgaaattatc aatcgctata tccgctttgc
    30901 agataaagac gacactaaac gtcggagtga ttggaaaact aatgaacgtt gggaatggtt
    30961 tatcggtaaa aatcgtggag cattacgcct aaccacacag ccagagcctt attcttttga
    31021 acgaacattg aactggttac atcatcaagt cgcccctaca ttaaaaatcg caagcatttt
    31081 agatgttctc aacggtacaa ccattatctc caccatgatt caggaagcca aactgacaga
    31141 aaaacatgag aaattaattg aacaacaaca tctagccatg gaggatttga ttacatgagc
    31201 cttttaacaa aagaattaaa gaagttaggg tttcagtgtg gcattgagtt tcaagcgtat
    31261 atacaaaata cagggaaata tacgtctctc attattgagg gaaaacggca agctggtgat
    31321 accatctata cctatgattt ttacaaggtg agattttata ataattatac caatcgagta
    31381 accgtctatg gagagcattt aacccctttt caattattaa ggcgtgtaaa aagctatatc
    31441 tattatcgag aaaaatatct taaggaaagg aggacaatca catgaatttt gggcagaatt
    31501 tgtacaactg gttcttggat aatgcacagt cattggtatt gttagcgatt gttgtcattg
    31561 gactgttttt aggttttaaa cgagaatttt caaaactcat tggcttcttg attattgctc
    31621 taatcgccgt cggacttgtg ttcaatgcag ctggcgtgaa agatgtgtta ttaaacttat
    31681 ttaaccgaat cattggtgct taacagatat ttttcatgat aaaataggaa gtatatattt
    31741 tgttatgaaa ggatttatga atatgtttac aaatgatcaa tttaaatctc ttttaattaa
    31801 aacattagaa agaaagagct cttcaaatta ttatgaagga ggtaacgaga ttgtttctaa
    31861 aatgaaaata tcagaggtaa ctttagatga gactgacgat tttacttatt ataaaggata
    31921 taaaaagggc tggaatacgc tttgcacata tcttaaaata agagttcctt ttgatgattt
    31981 agactttttt gaaagtcata aagatttgat tacccagaca gcaagctcca tatatgataa
    32041 acaaggtgat aatgtattag tggatactat tttagtacca cttccagaga attacgaagt
    32101 aattaacttt agtcagttga aaattagtga tgtggtttca caagctattg aagacgctga
    32161 gtcttttatg agtaatggtg aatatcaacg tgcctttgac agagttcaca ctgcttttca
    32221 tggatattta attgaaatac taaaaaaata tgaaattaca gttcctagag acgaaaatct
    32281 atctaaatta tatagtagga tacaacaatt aattgaaaaa gaaattcaac ctactgaact
    32341 tgctgatata gtgaagacaa cgataaggtc atcaaatgga atgattagct ctcttaatga
    32401 agcaagaaat aggcattcac ttgcacaccc taatacaaat attattggaa agagagaagc
    32461 aaaactaata ataggtattt caagtacagt tacagattat attagcggat accttgataa
    32521 gtagcgtcat taactgattg ctactttttt tatgactatt tttataaaaa ggatgtgata
    32581 agaattggaa atgaaagtct atgtggcaaa tttaggacgg tataatgaag gtgaattagt
    32641 cggagcatgg tttactccac caattgatga ggaagaaatg gcagaaagaa tcggtttaaa
    32701 tgaggactac gaagaatatg caatacatga ttttgagctt ccttttgatg tggacgaata
    32761 cacgccgatt agtgaaatca accgtctatg tgaagccatt caagaaatcg aaggtacacc
    32821 tatttataat gaactcaaag aaattcaagg catgtggttt agtagcttgg aagaattact
    32881 ggaaaacaaa gaggatattc attgttatag tgattgtgat tcgatggaag atgtcgcccg
    32941 ttattacgtg gaagaaacag gacagcttgg tgaagtccct tccaatttac aaaattatat
    33001 tgactaccaa tctttgggac gtgatatgga aatagagggt aattacctag ttacctctca
    33061 tggggtattt gaatattgtc agtaagttta acaatccgtg ttataattaa attaataaat
    33121 agcacggaga ggtataaatt atggtaaatg tatttgaaaa tcttaaacta ttaaaactag
    33181 acatgaaaga taaaggctgg gtgatagatt ccttttactt tcgttacaaa cagcaaaatt
    33241 atattgtact tgttaagcta tttgaaaaag aggaaaaagt tccagaatat gctctgttaa
    33301 aactagaatt tttaaaggaa aacgattttt cagatatgct agctgtctat gctaattcgg
    33361 taaaattata tacagacacg aaaacaataa gagaatattt tggtatcgaa tacagtagta
    33421 acttgggaga tgttttattt caatttagcc aaactcttgc tcgatttatt ccgacagaag
    33481 ttagtgagaa gaaaaatgaa gaccaaaaag aagccatgtg tttctcatta agtcaaagtg
    33541 attcagaaga tcccagaaaa aaatattgtt tttcagttag aagaaatcct ttaaagggaa
    33601 atggtgagct gggaaaacga agtcctttta atgacaataa aacgagattg tatagaccca
    33661 gcttatatga acgattaggg tcagatacca atttaagttt cagatattca atgaatccag
    33721 atgatgaaga aactgatgaa ggtattattg caaaatggac aaaaaataaa attgaatagt
    33781 taaagcattg aaagagcaat cacctatttt gattgttctt tttctatttt tacggaaagg
    33841 acgtgaagac atgaaaaaaa taagaagtta caccagtatt tggtcagtag aaaaagtgat
    33901 ttatgcgatt aatgatttac aactcccttt cccaattacc tttacgcaaa tgacatggtt
    33961 tatcgtttct ttgtttgtcg tcatactctt tggcagttta ccaccacttt ctctgattga
    34021 cggagcacta ctcaaatact tagggattcc cattgttctc actgggttca tgtgccaaaa
    34081 gacctttgac ggaaagaaac cctatggatt tctaaagtct atcattacct atgctctacg
    34141 ccctaaagta acctacgctg gcaaacaaat acgattaaaa aaagaaacaa tgaatgaatc
    34201 gattacgatt gtaaggagtg aacaactaga tggcatatcc gattaaatat atggaagata
    34261 atctcgtatt taatcatgat ggcgaagtat tcgcttatta tgaattaatt ccctataatt
    34321 acagtttttt aagttccgat gaaaaaattc aagttcatga taatttctgt caattgattg
    34381 cccaaaatcg tgatggcaag attcatgctc tgcaactagc aaccgaatca agtattcgag
    34441 ccacacagga acaatcgaaa aaagaaatca ctggacgatt aaaagagata gcttacgaaa
    34501 aaatagatga acagaccgaa gcgttaattt ctatgattgg tgacaatcaa gtagactatc
    34561 gcttttttat tggttttaaa ctctacctca atgaacagga agtatccgta aaaaatgttg
    34621 ggaaagaaat tcaaaaagca tttgcggaat tctttcatgg tgtgaatcac aaattaatgg
    34681 gcgattttgt ttctatgcct atggcggaaa tcgaacgctt ttctaagatg gaacagttgt
    34741 tagaaagtaa attatccaga cgctttcaga ttcgtaaatt ggagaaagat gattttggtt
    34801 atattattga acatctttac gggcaaacag gtgtcccttt tgatgactac gactaccatt
    34861 taccgaaacg atacgaagat aaaaaagtgt tggtaaagaa atacgatatt ctcaaaccca
    34921 ctcgctgttt aatcgaagaa aatcaacgct atttacggat tgaacaggaa gataccacta
    34981 cttatgtggc gtattttaca attaatagta ttgttggtga attggatttc cctaatagtg
    35041 aaatttttta ttaccagcaa cagcagttta ctttccccgt tgataccagt atgaatgttg
    35101 aaattgtcgc caataaaaaa gccttatcta ccgttcgcaa taaaaagaaa gaactgaaag
    35161 atttagatag ccacgcatgg gaaagcgaca acgaaaccag tactaatgtg gtggacgctt
    35221 tagaatctgt cgatgaatta gaaagtaatt tagaccaaac caaagaatct atgtacaaac
    35281 taagctatgt ggtacgggta tcagcaaaga atttggacga gctgaaacaa cgttgtgatg
    35341 aagtaaaaga tttttatgat gatctgtcta tcaaattagt acgacctttt ggcgatatgc
    35401 taggcttgca taatgaattt attccctcaa gcaagcgata tatcaatgac tatattcaat
    35461 atgtcacgtc ggattttttg gctggtcttg gtttcggtgc tacacaaatg attggtgaaa
    35521 attctggtat ttacgtcggt tacaatctcg atacaggcag aaatatttat ctccaaccta
    35581 gccttgcaag tcaaggaatt aaaggctctg taacaaatgc attagcgtcc gcttttattg
    35641 gttcattagg tggcggaaaa tcattctcta acaatatgct ggtctattat tcagtactct
    35701 ttggcggaca ggcaaccatt gttgacccaa aagctgaacg tggtgaatgg aaagaaacct
    35761 tacctgatat tgcccatgaa atcaatattg ttaatctcac gagtgagaac gacaataaag
    35821 ggttacttga cccttatgtg attttaaaac tcccgaaaga ttcagaaagc cttgccattg
    35881 atattctcac gttcttaaca ggtatttcca gccgagatag cgagaagttt cctgtcctgc
    35941 gtaaagctat tcggagtgta actcaaagca aacaacgagg gttgttattg gtgattgatg
    36001 aattgcgaaa agaagatacg cccatcagca atagtattgc tgaccatatc gaaagctttg
    36061 tcgattatga ttttgcccat ctgttattca gtgatggcag tgtcacccaa tccattagct
    36121 tggaaaaaca actcaatatt attcaagtgg cagatttggt cttacccgat tccaatagct
    36181 cttttgaaga atacactacc atggaattat taagtgtggc aatgctgatt gtcattagta
    36241 cctttgcttt agactttatc cactctgacc gttccatttt taaaattgtt gaccttgatg
    36301 aggcttggag ttttttacaa gtggcacaag gaaaagctct atctatgcga ttggttcgag
    36361 caggacgtgc catgaatgca ggggtctatt tcgttactca aaacgcggac gatttactag
    36421 atgaaaagat gaagaataat attggcttga aatttgcctt tcgttccact gacctagtgg
    36481 aaatcaaaaa gaccttagaa ttttttggtg tggacaagga agatgaaaac aatcagaaac
    36541 gcttacgtga attagaaaat ggacaatgtt tagtttcaga catctatggt cgtgtagggg
    36601 ttatgcagtt tcacccgatt tttgaagagt tgcttcatgc ctttgatacc agaccgcctg
    36661 tgcgaaatga ggtggaagaa tgacacaaaa acaaaagaaa tttcttcact atagttggat
    36721 aacgctcctt tgtgtgggcg ttattttact tttattaggc acgctgggca atgcagtaca
    36781 agcgacaggc ttagttgatg aaaccattga tacaagcaat gaatactcca agtatggttt
    36841 gaatcaatat cagcttgatt attatgtgga caatagctgg ggctggcttc catggaattg
    36901 gagtgatggc ataggacaat cggtgatgta tggattgtat gccattacta attttatttg
    36961 gacaatttct ctttatctat cgaacgccac aggttatttg gtgaaagaag cctatgcctt
    37021 agatttcatt agtcaaacat cccaagcaat tggcgaaaac atgcagacga ttgcaggcat
    37081 tacaccgaat ggcttttcca gttcagggtt ttatgtcggt ttcctccttc tttttattct
    37141 cattgttgga atctatgttg cctatacagg attggttaag cgagaaacga cgaaagtgat
    37201 acgagctgtt ttaaactttg tcgtggtgtt tattctatcg gcttccttta ttgcgtatgc
    37261 acccgattat attgcgaaaa tcaatgattt ttccaaagat gtcagtaccg ccagtctgaa
    37321 tattgggaca aaaattgttc ttcctaacac ggatagtcaa gggaaagaca gtgtggacat
    37381 gatacgtgac agcttattct ctgttcaagt aaagcaacca tggctattgc ttcaatatgg
    37441 ctccactgat gtagaagcgt taggggaaga acgtgtggaa agtttattgt ctaccagtcc
    37501 agatacaaac aatggtgaag atagagaaaa tgtagtgatt gaagaaattg aagatcatga
    37561 caatacctat ttaaccctac cgaagacgat ttccagatta ggaacagtct tctttttgtt
    37621 tgtattcaat attggtatct cgatttttgt tttccttcta acaggaatca tgattttttc
    37681 gcaagtgcta tttattattt tcgctatgtt tttgccaatc agtttcctat taagtatgtt
    37741 gccgagtttt gatggcatgg gcaggcatgc cattaccaag ttatttaatg tgattatgac
    37801 aagggctggt attaccttga ttattaccgt tgcctttagt atttccacta tgctttattc
    37861 gctgtcagca agttctccct ttttcatgat tatgttttta caaattgtga cctttgcagg
    37921 gatttatttt aaactgggcg atttaatgag cctattcact ttacaaagca acgactctca
    37981 aaacatgggc agacaagtgt ttcgcagacc tcgtcaaatg atgaatcgac aaaccagaag
    38041 atttcaacgc agtatgagta agatttttcg tggaaaagca gggtcaacga agcaaaagac
    38101 aatgacaaca aagcagaatc aaaccaataa agctaaccat actcgtcaaa atgaacggac
    38161 tacaccaaag aaagaaacag cactttccaa tgtgaaaaag caacaaggcg gtatcgggca
    38221 acgattaggt gaaaagacag cccatgtatt ggacacgaaa aatcgtctag tcaataaaag
    38281 aaagcaggca aaacaacaga tacaggcgac accaacccat gtcaaatatg cccttcatca
    38341 aggaaaagag aatgtcactc ataatgtaac agactttaaa caatctatgg cagataccaa
    38401 agctcaacgc aaacaacagc gtatgaacca gcaggagcaa aaacgaaaga acattgctca
    38461 aaagcgtttg gagctggata aagcaaaaga aaaaagacga aaagaaaacc catcatctcc
    38521 tgttcagaat aaccagcagc atgttcgcaa gcgtcagcaa accgtcacgc ctgttttgtc
    38581 acaaaacact aaacgtgaaa atcatcagcg accattgaaa aaagagcaaa aacaaaagac
    38641 accaccgaag caattcaatc agaggaagct aaataaaaga acggataagc gaggacaaca
    38701 aacatgaagc taaaaataac agtgatttgt agtgtggtct tctctctctt cttttttctc
    38761 ttattattcc tagtcatttt ctttgcagat gatacggaca gtggcgaaaa caacaaggat
    38821 tcttctattc cacaaggcgg tgtgaccgtt tcaccagaag tgttggcaca ccgtccgttg
    38881 attgaaaaat atggtaagga atatgggatt gaagactatg tttcttatat cttagccatt
    38941 atgcaagtgg aatcgggcgg tacagcagaa gatgttatgc aaagttccga atctttgggt
    39001 ttaccgccca acagtttgag taccgaagag tccatcaaac aaggggttaa atatttcagt
    39061 gaattattaa ccagtgcaga acaacaagga gtggatatag attctgttat ccaatcttat
    39121 aattatggcg gtggcttttt aaattatgtg aggagtcatg ggaaaaaata tacctatgaa
    39181 ttagccgaac agttttctaa agagaagtcg ggtggtcaaa aagcagacta tcccaatcct
    39241 atagccatac ctgtgaatgg tggttggcga tataactatg gaaatcagtt ttatgttcag
    39301 ttggtttcac aatacctaac cgacacttcc ccaacagaat ttgatggtga aaccgtgcaa
    39361 gtcattatgg acgaagcctt gaaatacgaa gggtttccct atgtctttgg aggggcgtcg
    39421 cctaccacct cttttgattg tagtggctta atacaatggg tgtatgacaa agctggcatt
    39481 tctctccctc gtgtcgccca agatcagtat gacgctacgc aagaaatctc tatggaagaa
    39541 gcccaagcag gcgaccttat cttctttcat tcaacctata atgcaggcac atatgtgact
    39601 catgtggcga tatatttaga gggaaatcgc ttttatcatg caggcgaccc aatcggttat
    39661 ggcgacttaa gcagtcgtta ttggcaagac catctgattg gggctagacg tgttattcat
    39721 aactaagaaa ggaatgaatc aaatgattat aaaaatcgag cgaaaagaaa agaaggagaa
    39781 accaaagaaa tctaaaaaac cgagcaagca aagaaaagtt ccgatgatta aagttggcac
    39841 gcataagaaa ttaacctttg ttttatgggt tttattgatt ggtagtgtcg gatttggaat
    39901 ctataaaaat tttactgcca ttgacactca tactgtccgt gaaacagaaa tcattaaaca
    39961 gcagattgtc gataccaacc aagtagaaag ttttgtgaaa tcctttgcga aagactactt
    40021 ttcgtggcaa caatcccaag aagccattga taaacgaaat gaaaaattga cccattattt
    40081 gacggaagaa ttgcaggtat taaatgaaga aatgattcgt aaggatattc ctactagttc
    40141 tagtgtgaac gatatacagg tgtggcaggt ttctcaagtg aatgagaaca cctttgaggt
    40201 actgttttct gttgaacaag ttattaccga agacaaagac aaggaaacta tatcgtctag
    40261 ctttcatgtg gttgtccata tagatgaatc agataatatg gttattatca aaaatccgac
    40321 aatgagtaag aaacctcaaa aatcggacta ccaacccaaa cagctagaaa gtgaccatac
    40381 agtagataca gaaacgatgg acgaaatcac tagcttttta gaaacgttct tccagctcta
    40441 tcctaccgcc acagaaaaag aattgactta ctatgtaagt aatcatgtgt tacctatgat
    40501 taacaaggaa tatgttttcg aggaattggt gaatcctatc tttaccagaa aagataacca
    40561 agtaatcgtg aatgtcgctg tgaaatattt agatcaagaa acaaaggcaa cccaaatctc
    40621 gcaatttgag ttgatattag aaaaacaaga taattggaag attgtaaaat aacaaatatt
    40681 ggtacatgat tacagatact ttgtaatcat gtactctttt tgataaaaaa ttggagattc
    40741 ctttacaaat atgctcttac gtgctattat ttaagtgact atttaaaagg agttaataaa
    40801 tatgcggcaa ggtattctta aataaactgt caatttgata gcgggaacaa ataattagat
    40861 gtcctttttt aggagggctt agttttttgt acccagttta agaatacctt tatcatgtga
    40921 ttctaaagta tccagagaat atctgtatgc tttgtatacc tatggttatg cataaaaatc
    40981 ccagtgataa aagtatttat cactgggatt tttatgccct tttgggtttt tgaatggagg
    41041 aaaatcacat gaaaattatt aatattggag ttttagctca tgttgatgca ggaaaaacta
    41101 ccttaacaga aagcttatta tataacagtg gagcgattac agaattagga agcgtggaca
    41161 aaggtacaac gaggacggat aatacgcttt tagaacgtca gagaggaatt acaattcaga
    41221 caggaataac ctcttttcag tgggaaaata cgaaggtgaa catcatagac acgccaggac
    41281 atatggattt cttagcagaa gtatatcgtt cattatcagt tttagatggg gcaattctac
    41341 tgatttctgc aaaagatggc gtacaagcac aaactcgtat attatttcat gcacttagga
    41401 aaatggggat tcccacaatc ttttttatca ataagattga ccaaaatgga attgatttat
    41461 caacggttta tcaggatatt aaagagaaac tttctgccga aattgtaatc aaacagaagg
    41521 tagaactgta tcctaatatg tgtgtgacga actttaccga atctgaacaa tgggatacgg
    41581 taatagaggg aaacgatgac cttttagaga aatatatgtc cggtaaatca ttagaagcat
    41641 tggaactcga acaagaggaa agcataagat ttcagaattg ttctctgttc cctctttatc
    41701 atggaagtgc aaaaagtaat atagggattg ataaccttat agaagttatt actaataaat
    41761 tttattcatc aacacatcga ggtccgtctg aactttgcgg aaatgttttc aaaattgaat
    41821 atacaaaaaa aagacaacgt cttgcatata tacgccttta tagtggagta ctacatttac
    41881 gagattcggt tagagtatca gaaaaagaaa aaataaaagt tacagaaatg tatacttcaa
    41941 taaatggtga attatgtaag attgatagag cttattctgg agaaattgtt attttgcaaa
    42001 atgagttttt gaagttaaat agtgttcttg gagatacaaa actattgcca cagagaaaaa
    42061 agattgaaaa tccgcaccct ctactacaaa caactgttga accgagtaaa cctgaacaga
    42121 gagaaatgtt gcttgatgcc cttttggaaa tctcagatag tgatccgctt ctacgatatt
    42181 acgtggattc tacgacacat gaaattatac tttctttctt agggaaagta caaatggaag
    42241 tgattagtgc actgttgcaa gaaaagtatc atgtggagat agaactaaaa gagcctacag
    42301 tcatttatat ggagagaccg ttaaaaaatg cagaatatac cattcacatc gaagtgccgc
    42361 caaatccttt ctgggcttcc attggtttat ctgtatcacc gcttccgttg ggaagtggaa
    42421 tgcagtatga gagctcggtt tctcttggat acttaaatca atcatttcaa aatgcagtta
    42481 tggaagggat acgctatggt tgcgaacaag gattatatgg ttggaatgtg acggattgta
    42541 aaatctgttt taagtacggt ttatactata gccctgttag tactccagca gattttcgga
    42601 tgcttactcc tattgtactg gagcaagcct ttagaaaagc tggaacagaa ttgttagagc
    42661 catatcttag ttttaaagtt tatgcaccac aggaatatct ttcacgggca tataacgatg
    42721 ctcccaaata ttgtgcaaat atcgtaaata ctcaactgaa aaataatgag gtcattatta
    42781 ttggagaaat tcctgctcga tgtattcaag attatcgcaa tgatttaact ttttttacaa
    42841 atgggcttag tgtttgttta gcagagctaa aaggatatca ggttaccact ggcgaacctg
    42901 tttgccagac ccgtcgtcta aatagtcgga tagataaagt aagatatatg ttcaataaaa
    42961 taacttagtg cgttttatgt tgttatataa atatggtttc ttattaaata agatgaaata
    43021 ttctttaata tagatttgaa ttaaagtgga aaggaggaga ttgttattat aaactacaag
    43081 tggatattgt gtcctatttg tggaaataaa acaagactac gaatacgagt ggatactata
    43141 cttaaaaatt tccctttata ctgccccaaa tgtaagaacg aaactttaat taatgttcaa
    43201 aaaatgaata taataacaat caaagagcca gacgccaaga cgcagagccg ataatttgag
    43261 aaatgaaact ctcatcttat cggctctttt tgtttatctg aattttactg actagccttc
    43321 aatatttctt gcttcattga taccttttgc tagtgcttcc attaaggata gttctttgtc
    43381 tgtaaagcta tccatgtatt tttctacctg taaccttcga gtgcttttaa tcaaattatc
    43441 tgtaggtaag aaaaattcat caatagatac atggagtaat gatacaagat catacagcac
    43501 ttgtgtactt gggtgttgtc ctttgttttc tatatttgtt aaataacgtg ggtcaatttc
    43561 aatcattgtt cccacttgtt cacgagtgag accccgtttc attcgagcct ctttaatcgc
    43621 taaaccaacg gctctaaaat cgtatttatc ttctttttta cgcatactaa atcacctcta
    43681 tacattttac tgttcctata taaccataaa caggtataga aaaacatgtt atatagttta
    43741 tgagttccta tagaacactg tggaatttat taatgcgatg aatccaattt caaatagaaa
    43801 ggtcgctgat tttatttacc tatacaactt tataaaattt agtcatactc ctagaaagta
    43861 gataaaattg gcgacttaat ggagggaaaa gtcgccatga gaaagataat ctgtcatcta
    43921 gttctcttaa ttaagttctt atcaaaaaaa cttatccacc tgtactggat agatatgacc
    43981 atcaatgtgg ttttgcagat tacatagata gaaaaatcaa agcatatgaa cagagaaaac
    44041 aatctgttta tgtgcttttt tggtcactta ataaatatct tttaggattt ctcttcattt
    44101 atgcaacaaa tccccctttc gtgtcggggt ttagtatgaa gggagataaa gaagcacatg
    44161 ccactccctt tcacgacaga aagggggtga aaagcatgaa accatcttct tttcaagaaa
    44221 ccatagaaaa tcagtttgac tatatctgca aacaagttat tgaagatgaa cgaaaagatt
    44281 acttcaaaca tctagacaga ctatcagcaa aagaagtctc gttttctgat atagataact
    44341 accttgtcaa ctctttttct actgttgata cgtacgtcac tgatttccaa ctatttaaat
    44401 tatttggatt aagaatcaat gttgaaagtg atttattaag tgaagccctg cgtcatcttt
    44461 cagagaaaaa acggaacatt attttacttc attactttat ggatatgagt gatgtggaga
    44521 ttgcggagtt actaaatttg aatcgttcga ctgtttatcg tcatcgaata agtggactag
    44581 ccatgattaa agaattcatg aaggagtgta aagaatgaaa acagcttatc caagggttcc
    44641 cttccccctc attgtaaagg cgacagatgg cgacgtggaa gcaattaatc agattgttaa
    44701 acattatcga gggtatacct caaaacgttc attacgtcgc atgacagacg aatatggaaa
    44761 ttctcatatg gttattgatg aaaccctacg tggcagaatg gaaacgaaac tcatctctaa
    44821 gattttatcg ttcgaaatta aatagtccca ttttttcgtg gaagcgtgtc gaatcgtatc
    44881 cacgcttccc gatttgggag gtttgttatt ccatcaaagc atattgtgtg ttcagtaggt
    44941 tttgataggc taacaagcca aacgttcttt gacaaccaaa taaaggcaat cgaatacgtt
    45001 tcggtaagaa aagagctgat gaactaacac gccatgacct acaaaattag tgagcgattc
    45061 atgttagtaa ttcgaaagta atctttagca ggattgctgc catgactttc ttatcgtgat
    45121 aatgatactc ccgtatagcc atagtccgag cgttagaagc gtcacaggca atgggtacgg
    45181 ctacatgaga accatgcaga agtgaaattc ctgtgagctt tgctagagct gttcgattgc
    45241 taagatattc ttctcttgtc agtgtattat tataaaacta tttgatataa ttataggtga
    45301 atattggagt tttcctagcc tataattggg aaaggagtaa taatgataac ggtcacaaaa
    45361 aaggatttag ttgagctagg atatggaact tctttttcag cagacatcat aaaaaaagca
    45421 aaagaattga tgattacaaa agggcatatc tactatcaat ctcgaaagct agatagagtc
    45481 cctcgtgaag ctgttgaaga aattctagga attcggtttt caaatgaaaa caggtctgag
    45541 tagtttttgc acagtaagga gtaaaaacta tggttaaaga cccaataaaa aaagcaaaga
    45601 atggaaccta ttattttcgt gcaaatctag gttatgattc taaagggaaa aaaattcaaa
    45661 aatatcgtag tggattcact actaaaaaag aagcaagaga agaatattcg aaactgctat
    45721 tgatgaaacc agaagaatta agtgagaata aggataaaat gacgtttaaa cattatatct
    45781 ttgaaatttt ccttccatgg tataagacac aggtaaagct cagaacatat gaaaatcgct
    45841 taccaactat taagaagcat ttctcttatt ttgataaaat ggctgtttcc gatattgagc
    45901 ctattgatgt gcaaaattgg caactaaaat tatcaaagaa gtgtaagtct tcttatgtaa
    45961 gagctgttca aggactattt tcagttgcta tggatcgagc aattgtcctt gggataacca
    46021 ccacaaatcc atctaagatt attggcaatg tgaaaaagca aaagtctaaa attgaatttt
    46081 ggacaaaaga agaatttgaa aaagtaatct ctctaattta caaagaagat tactatcaac
    46141 actttttatt tatctctctg tggtttttat ttatgactgg aatgcgaatt ggtgaagcta
    46201 ctgccattca atgggaagat attgattttg actcaggagt actaaccatc aacaaaacac
    46261 tctattacaa gaatcaatcg aattatagat ttgttgaacc aaaaacaaag gcgagcgttc
    46321 gtcatattgt attagataaa tgcacattaa cttatttatc cgaatggaag gaagtacaac
    46381 aaaatctaat tcagacagac tttgtaatga gctataacgg tattcctacc caaaaacata
    46441 cactatcata tgctattgaa cgctatgcta agctggctgg tatccatcga atcagaatcc
    46501 atgggttacg gcattctcat gcttccttgt taatcagtat gggagaaaat ccacttatca
    46561 ttaaagatcg tctaggacac gaagatattg agacgacttt aggaacgtat ggacatcttt
    46621 atcctaacag taatttcgag gtagctaaca aattaaatgg agccatttcc tttaaagaag
    46681 caaaacaaaa ttatgatact tcaccaaaga atcaatttac tgtagactac ttgcgaaaca
    46741 aagaaatgaa aaaagtgcaa taaaagtgca gtaaaaagat aagaagagcc agaaaccctt
    46801 ggtataagag gatttctagc tctcctacaa ctattcccac tccactgttg ctggtggttt
    46861 agaagtgatg tcatacacga tacggttgac gtgggctact tcgtttacga tacgtacaga
    46921 gattttttgc aagacttccc atgggatacg ggcaaagtca gcggtcattc cgtcgattga
    46981 agttacagca cggataccga ctgtgtagtc gtaagtacgt ccgtcaccca taacaccgac
    47041 agagcggatc cctggaagaa cagtgaaata ttgccagata tcacggtcaa gtccagcagc
    47101 tgcgatttct tcacgcaaga tagcatcaga ttcgcgtacg atttctagct tgtcttcagt
    47161 gatttcgcca agaacacgaa taccaagacc aggaccaggg aatggctgac gccaaacgat
    47221 tgaatcaggc atgcctaact gagtccctaa ttcacggact tcgtctttga ataatgtgtt
    47281 taatggttcg atcaattcaa attgcatatc ttctggcagt ccacctacat tatggtgaga
    47341 tttgatcgtc tgtgctgttt ctgtaccaga ttcgataacg tcagtataca acgttccttg
    47401 tgctaggaaa gagacgcctt cttcaccagc aagttttgtt gcttcgtcat caaaaacata
    47461 tacgaattca ttcccaatga ttttacgttt ttgttctggg tcagatacac ctttcaactt
    47521 gtctaagaag cgatctcttg catcgacttt gatgatgttc aagccgaatt ttccgcctag
    47581 actttccatt acttgttccg cttcgccttt gcgtagcaga ccgtggtcaa caaagataga
    47641 tgtcaattgg tcaccgatcg ctttttgtaa aagaacccca acgacactag aatcaacgcc
    47701 acctgaaaga ccaagcaaga cttttttgtc accaacttgt tcacggattt tttctacttg
    47761 catatcgata aagttatcca ttgtccagtt accttcacag tgacaaacat caaaggcaaa
    47821 gtggcgcagc aattcgattc cgtattcgga atgacgtact tctgcatgga attggatacc
    47881 ataaaaatta cgtgttgtgt cttggataga agcaatcgga caatctttgc ttgttccgac
    47941 tgtttcaaag ccttcaggaa ctttagttac caagtcacca tggctcatcc atactgtttg
    48001 ttgttttggt gtttctttaa acaagacagc ttgatcgctt gttacttcta acaaggcttt
    48061 cccatattcg cggtttttag caggctctac ggaaccgcca agtttgtacg tgatcaattg
    48121 cataccatag caaatcccta atacaggaat acctaaattg aagatctcag gatcgatccc
    48181 aaatgcattc tcatcatata cactgtttgg tccgccagaa agaatgattc cctttggatt
    48241 catcgctttt acttcttctg cagtaatgcg gtggctcaat aattcggaaa agactccaag
    48301 atcacgaata cgtcttgtga tcaattggtt atactgacta ccgtagtcta atacgataat
    48361 cttttcaacg ttcgtcatat cggcaacgtt tgtcaaattt attaccccta tccctttaaa
    48421 ttttatctca aaatagtcaa actattttaa aatgctaata ttttcgcatg aaaatcgaat
    48481 cgatgatatg attttcagtt ttatggagaa ttatatcggc tcttcctcgt gtaggcaaga
    48541 tatattcctg taaatttttc aaattcacat ttttccatac atcttttgcc attttaaagg
    48601 cttcttcacg atctccgata gcaaaacgat aataataatt ctctggattt tggaaagccg
    48661 tatccagtaa cgcaccaaaa cgatccaaat accatttttc gatcagcacg ggatcggcat
    48721 cgacatagac agaaaaatca aaaaagtcgc tgacgtagat ttgctgattc gcaggtaatt
    48781 gaagcgtatt gatcccctca acgatcaaga tatcaggctg ctggatcaat tcgtacttgt
    48841 ctggaatgat atcatacaca tcatgtgagt aaacaggtac ttttatctct tcctgaccag
    48901 attttacacg gttcaagaaa tcgatcagca tctccatatc ataactttca ggaaatcctt
    48961 tacggtccat gattccacgc tcttttaata tacgatttgg ataaagaaat ccgtcagtcg
    49021 tgatcagctg aacgtttctt cgtttgaagg tacgagacaa aatcgtctgc agcagtctgg
    49081 ctgttgtact ttttccaacg gctacacttc cggcgatccc gataataaaa ggcggtactt
    49141 ctacgtattt atgaagaaat aaacctttac tcaagaacaa agattcaaat tctttcatat
    49201 aaagataaat cagatgagca agaggtacat agatatctcg aacatcctgc atggaaatgc
    49261 ggtcattcaa acttttgata ttatctagtt catcttgtgt caaaggtgct tgcccattac
    49321 gataaaattc tcgccattct tctcttgcca cacggtagta gttcattgga tcattcatat
    49381 tagcctcctg cgttctccca tcaatggaac tattttaaca caaaataaac gcaaacccca
    49441 ccacttaaga gagattgccg atctttttgt ataatcttta taatagaaag aaacggagtc
    49501 ttattcttga cttgcgcacg gatacataaa aggatagact gttgtcatga aagaaatgag
    49561 aaggatggaa gacatgacaa atcattacta ttctgaacgc ccagatacgg cgcacgattt
    49621 tgaacaatgg gcttttgagt taaaaggcag aacctttcaa tttgtaacag attctggtgt
    49681 attttcacgt gaaacagtgg attatggttc acgtgtcctg attgatgctt tcgattggga
    49741 agagttacct gatgaaggaa agatcttaga tgtgggatgc gggtatggac cgattgggtt
    49801 agctttggca tttgcgacac agcggtttgt tgaaatggtc gacattaatg aacgagcggt
    49861 aagtctagcg caaggaaacg caaagcgtaa ccaaatcgaa catgttgata tccatcaatc
    49921 gaatatctat gaagcagttc atgaagaaac ctatgcagct atcgtaagca atccgccgat
    49981 ccgtgctgga aaaaaagttg tacatgaaat tttgacaggc gcatatcctc ggttgaagaa
    50041 gggcggtaca ctgacaattg tgatccagaa gaaacaagga gcgccgagtg cgcaaaagaa
    50101 aatggaagaa acatttggta atgcagaaat cgtcacaaaa gataaaggat attatatcat
    50161 caaaagtgtg aaagaataac tagctgaaaa atagtggagc gacgaaagag tggaactggg
    50221 cgtgcatgta cgggtacgta tcagttcttt ttttgttgag aagtttttaa aaacaaagaa
    50281 ctattttttc ttcaaaaaga aatagtagga acagagtcgt tgttttttac gataaaataa
    50341 ataagataca gaacttgtat agaaggctgc caagtcggta gataaaggag gtttttatca
    50401 aaaaacatac tttccttttt tctctattct tttcttgggg gttttggtac actgataggg
    50461 aatttagata gaagggagcc aataggatga agctgaccat cagagatatc gcagaaatgg
    50521 caggcgtatc agtaacaact gtctcacaga ttttgaataa caaaggaagc agatttagtg
    50581 ataagacaag aaaaaaagtg ttgtcgatcg tgaatgaaca tcattataag cccgattatt
    50641 tcgcttcaaa tgtgatcaat cgacattcaa agaccatcgg aatgatcgtc cctgatgtca
    50701 ctgatttctt cttctccaaa ctgatcgaag gtgtagagag ctatttgaat cctttgggct
    50761 atgtgatcat gttatgcaat tctcgtcata gccaagaaaa tgagatcaag tatttacaag
    50821 agctttccca ccgctcagtg gacggaatct tgctggccac accaaatatt ttacctgaga
    50881 aatatgcatt agacagcggt ttttatcaaa aaatgccaat cattttaatc gatcgcggat
    50941 tgaatcgtcg ggataacgga cggcttatcg taaaggaata tgagggcgcc tatcaagctg
    51001 tttcgctgtt tattgaaaat ggccatacga agatcggcat gctgaaggaa accactggat
    51061 actatcaatt agaagaacgt ttcaacggat atcgtcatgc gctgaaagat catggcctgc
    51121 cttttaatgg gaaatttgtt gaacatggag agttgactgt ccaaggtgga tatactgcat
    51181 caaaaaaatt actcaaacat aaagatatca cggctatttt ttgcgggaat gatgccatgg
    51241 cgatcggatg ctatcaggcg atcgatgaat tgggcaaaaa aatcccagaa gatatctcag
    51301 tcataggatt cgatggatta aagctgtcag aatacatgat tccgcaattg acgacagtgc
    51361 agcagccaag ttttgatatt ggattttatg ctgcacgttt cctgatcgat acgatcgaat
    51421 ttcctcaaag aaaagttccg aacaaagtgt tcgaaacaaa attaattatt cgtgagagcg
    51481 taaaagtatt gacaaagggc taactcggcg gtatattgct tatgtaagca tttacaaagg
    51541 ctagttagtg ctttttttat ttttagtaaa ccagtttact aaagtggttt acctttccta
    51601 aaaataatgt ataataccga atgaagacaa ggaggatcag gcaatgcgaa tggtggatct
    51661 gattgaaaaa aagagggatg gaaacgagct gtcgaaagag gaaatcgagt acatcgtcac
    51721 gaactatacg aatggaaaga ttccagatta tcaagtcagt gcattattga tggcaatttt
    51781 ttatcaagac atgacaaatg aagaaatcac gaacttaaca ttggcgattg ctaattctgg
    51841 tgatgtcatc gatttgagtt ctcttgaagg aataaaggta gataagcatt cgacaggtgg
    51901 cgttggtgac acgaccacat tgattttagc ccccctagta gctagtgtcg gagtgacagt
    51961 ggctaaaatg tctggacgcg gtctaggtta cacaggtggt acactagaca agctagaagc
    52021 aattcccgga tttcagatcg agctttcgga tgaagaattt gtacgcattg ttaatgaaag
    52081 taaagtagcg gtaattggtc aatcaggaaa cttggcaccg gcggacaaaa aattgtatgc
    52141 cttgcgtgac gtcacagcga ccgttgattc tcttccgctg attgccagtt cgattatgag
    52201 taaaaaaatc gcggcaggag cagatgcaat cgttttggat gtgaccactg gagacggtgc
    52261 tttcatgaag aacatcgaag atgcaagacg tctggcaaag accatgacga gcattgggaa
    52321 actggctaac cgagaaacag tggcggttat ctcagatatg tcagaacctc ttggcgaagc
    52381 tatcggaaat agtctagaag ttgtcgaggc aatcgaaaca ttacaaggaa atggtccaga
    52441 agatctagta gaaatgtgct acgccttagg aagccaaatg gttgttcttg ctggcaaagc
    52501 taagacaacc gatgaagcaa gaacgttgct tcaagaagct ctggaatcag gtaaagcgtt
    52561 agcaaaattc aaagaaatga tccaaaatca agggggagac ccagcgatcg tggaacatcc
    52621 agaacgaatt ttgactgcac gctacacaat ggagcttcct gctaaacaaa gcggagttgt
    52681 gtcaaaaatc gtggcaaatg aattaggaat tgctgcaatg atgctaggtg ctgggcgtaa
    52741 aaccaaagaa gaagatattg atcacgctgt tggattgaaa ctgcataaaa aaattggtga
    52801 cactgtgaca aaaggtgagt cactattgac gatctatagt aacgacgaag aaatcacttc
    52861 tgtcattgaa ttattatata aaaatattga aattggtgaa tcggcaatga agccaacgtt
    52921 gattcatgac atcatcacag aataaaggaa aaaagcggac tttaccgttt taaagaaaag
    52981 gggaaaagaa atggaactaa accgaatgat cgaccatact attttgaaag cagacgcatc
    53041 taaagaagct gtgatgcaaa ttatcgaaga agcaaaaaaa taccattttt attctgtctg
    53101 catcaatcca gcatgggttg cgctagcgaa agaacaatta caaggtgagc cagtagccgt
    53161 atgtacagta atcgggtttc ctctaggagc aaacacaccc gagacaaaag catttgaaac
    53221 aacagatgcc atcaataacg gtgcagatga agtcgatatg gtcatcaata tcggtgcttt
    53281 gaaatcaaag gatgatcaac aagtccaaaa agatatcgaa gcagttgttg aagcagcaaa
    53341 agatcgtgcg cttgtcaaag ttattattga aacatcgtta ttagatagag aagaaatcat
    53401 ccgagcttgc gaaatagcaa aagctgcagg cgctgatttt gtcaaaacat caactggttt
    53461 ctcaacaggc ggagcaaagg tagaggatgt ccgtttgatg cgtgaaactg taggaccaga
    53521 aatgggtgtc aaagcttccg gcggtatcca taacgaagaa gaagcaatgg caatgatcga
    53581 agctggtgct acaagaatcg gaacaagcgc aggagtagca atcgtatctg gttcaactgg
    53641 tgaaggctat taattttttc aaaggagaag ctcatggcag aactcaaaca agaatggatc
    53701 aacacagcta ttgaagcgtt agataaagcc tatgttcctt attcccattt tccggtaggc
    53761 gcttgtctcg taacggaatc aggcaagatt tatcaaggca tcaacattga gaatgcatca
    53821 tttggattaa cgaattgtgc cgagcgcaca gcttttttca aagcagtatc tgaaggagaa
    53881 aaaacattta cgcatctagt cgtagcgggg cacacccccg atcctatctc tccttgtggt
    53941 gcatgtcgac aagtaatggc agaattctgt tcccctgata tgccggttac gctagtagga
    54001 gacaatggtg tgacgaaggc aacgaccgtt cgtgcactgc tgccttatgc atttactgaa
    54061 aaagatctat aactcatgtt cattcagtca gctgactgtt gaaaaataaa acaattattt
    54121 taggaggctt tacagaaatg aaaaaagcaa aattatttgg tttaggtgct gtcgcactag
    54181 cagcagggtt atttttaggt gcatgcggca acaacggctc tactgattca tccggtggca
    54241 aaagctcatc agacacaaca actgcagcat tgatcacaga cacaggtggt gtagacgacc
    54301 gttcattcaa ccagtcagct tgggaaggac tagaaaaatg ggggaaagat caaggacttt
    54361 cacgaggaaa tgatggcttc caatatttcc aatcttcaaa tgaatcagat tacatcccaa
    54421 atattgacca agcattgaat gctggattca aaacgatttt tggtattggt tataaactaa
    54481 aaccagcgat ccaagaacaa gcaacaaaca atacaggtac gaactttgtt attatcgatg
    54541 atgtcatcga tggattagat aacgttgtat ctgcaacttt caaagacaac gaagcttctt
    54601 atcttgcagg tgttgcagca gcttatacga ctgaaacaaa cgtagtcggc tttatcggcg
    54661 gtgtaaaagg tgaagtaatc gatcgttttg atgctggatt caaagccggt gttgatgcag
    54721 gtgcgaaaga attaggaaaa gaaatcaaag tattgaatca atatgctggt gacttctctg
    54781 ctccagataa aggacgttca attgctcaag gtatgtatgc acaaaacgca gatatcatct
    54841 tccatgcttc aggcggtaca ggtaacggtg tattccaaga agcaaaatca ttgaacgaat
    54901 caggcgacaa aaaagtttgg gttatcggtg ttgaccgcga ccaatcagac gaaggcgaat
    54961 acacattaaa cggcgaaaag aaaaacttca cattgacatc tacactgaaa gcagttggaa
    55021 cagtagtaga agatttggca caaaaatcag cggacggtaa attccctggc ggcgaacaca
    55081 ctgtttatgg attgaaagaa gacggtgttg gtctaacaga aggacaactt tctgacgaag
    55141 cgaaaaaagc agttgatgaa gcaaaagaaa aaatcatttc aggtgacgta aaagttccag
    55201 aaactcctga agaaaactaa aaccatttct atagccttgt tagacacatg tttgacaagg
    55261 ctatggtttg attagaacaa gaattgttca tttatccaag ctgccttgtg acagtttgga
    55321 taagggaaca actccctgaa gtaatgtttg tttcaaaaga agaacgctca ctaagcgtga
    55381 atcttagtca atcgttgccc tagatagaaa tcgttaggat gtgaaaaaaa ggtggttcaa
    55441 gaaaatattg tcatagaaat gcagaatatt accaaagaat ttggaacatt caaggcaaat
    55501 gacaacatca atttacaagt aaaggcagga gaaattcatg cgttgcttgg agaaaacggt
    55561 gctggcaaat ctacattgat gaacgtgctt tccggattat tagagccgac atcagggaaa
    55621 attttgatgc gtgggaaaga agtaaagatc acaagtccga ccaaagccaa ccaattaggg
    55681 attgggatgg tccatcagca ctttatgctt gttgatgcct ttactgtaac agaaaacatc
    55741 gtgttgggaa gcgagcctag tcgcgcaggg atgcttgacc ataaaaaagc acgaaaagag
    55801 atccaaaaag tttctgaaca gtatggattg tcagtcaacc cagatgctta cgttcgtgat
    55861 atttcagttg gaatggaaca acgggtagaa attttaaaaa cactttaccg aggagcagat
    55921 gtactgattt ttgatgagcc gacagctgta ttgacccctc aagaaattga tgaattaatc
    55981 gtgatcatga aggaattagt caaagaaggc aagtcgatca ttttgattac gcataagtta
    56041 gatgaaatca aagcagtagc tgaccgctgt acagttatcc gccgtggaaa aggaatcggt
    56101 acagtcaacg ttaaagacgt aacctcacaa caattagccg atatgatggt tggaagaacg
    56161 gtttcattca aaacgatgaa aaaagaagcg aagcctcaag aagtcgtttt gtctattgaa
    56221 aatctagtgg tgaaagaaaa tcgtggatta gaagccgtga aaaacttgaa cttagaggtt
    56281 cgtgctggcg aagtacttgg tatcgctgga atcgatggaa acgggcagtc ggagttgatc
    56341 caagctttga ctggtttgcg aaaggcagaa agcggacata tcaagctaaa aggggaagac
    56401 atcactaata aaaaaccccg aaaaatcact gaacatggtg taggacacgt gccagaagac
    56461 cgtcataaat acgggttagt cctagatatg acattatctg aaaatatcgc actacaaacc
    56521 tatcatcaaa aaccttacag taaaaacggc atgctgaatt attcagtaat gaatgaacat
    56581 gccagagaat tgatcgaaga atatgatgtt cgaacaacga atgaacttgt tcccgcaaaa
    56641 gctttatcag gcggaaatca gcaaaaagca attatcgctc gtgaagtcga ccgagatcct
    56701 gatctgttga ttgttgcaaa tccaactcgt ggactggatg taggtgcgat cgaatttatt
    56761 cataaacgtc tgatcgaaca aagggacaaa tacaaagcag tgttactgat cagtttcgaa
    56821 ttagaagaaa ttttaaatgt ttcggatcgt attgctgtta tccatgaagg agaaattgtc
    56881 ggtatcgttg atccgaaaga aacatctgaa aatgaattag gtctattgat ggcgggctat
    56941 accttagaag aagcacgaaa agagttagaa aagggggctg gcgcacatga atgatcgcag
    57001 cgaaaaattc cgtaatttat tagtgcctgt actttcagtt gttttaggat tgatcttagg
    57061 cgcgatattg atggctgctt ttggttttca gcccatccaa ggttattctt ctatgctaga
    57121 tgcagccctt ggttcgcaaa gagggatagg tgaaacgtta aggcaagcaa cgccgttgat
    57181 ttttacagca ttaggttttt ctgtagctaa ctcagctggg tttttcaata ttgggctttc
    57241 tggacaagca ctttgcgggt ggatcgtcag tgtctgggtg gcacttgcgt ttccagatct
    57301 gccaaaagtt gttttgcttc cgttatgcgt gatcttaggt gccttagctg gtgcgatggc
    57361 agcggctgtt ccaggtttac ttcgagcatt ttttggaaca agtgaagtga tcgtaacaat
    57421 catgatgaac tatatcttat tatacgtaag cacatttgct ttacatgaag tgatgcctga
    57481 aagctatcgc tcaagtttgg actcttccaa tatgatcagt caaaatgcct cactgcggtt
    57541 accatggtta acacaagctt ttggcggctc tcgcgtaaac tcaggattat ttttagcaat
    57601 catcgcgctt attttcgttt ggctgctgat gaagaaaacg actttaggat ttgaaatccg
    57661 ttctgttgga ttgaatcctt ttgcttctga gtatgcagga atgagtagta aacgtacgat
    57721 cgttttgtcg atggtcattt ctggcgcttt ggcagggctt ggcggtgtgg ttgaaggatt
    57781 aggaacgtat caaaacttct ttgttcaaac gacttcgcta tctattggat ttgacggaat
    57841 ggctgtttcc ttgcttggtt ctggatcagc aatcggtatt cttttatcag cgatcttgtt
    57901 cagtattttg aagatcggtg gacttggcat gcagacagga gcaggtgttc cgtttgagat
    57961 cgtcaatgta tcgattgctt tgattatctt ctttgtcggg atcaacttcc tgattcgttt
    58021 cgtcatggct aaagtcttcc aagggaaaaa acaagaagag atcgttgcga caatagaatc
    58081 gaaaccaacg aatcaaaacc atgaaggggg cgaactataa tgtcgactat cgaattaatt
    58141 gccttgatcc ttacttctac actggtttat tcaacaccgc taattctaac gtcactaggc
    58201 ggaacttttt ctgaacgaag cgggatcgta aacgtagggc tagagggaat catggtcatg
    58261 ggtgctttta gttccatcgt tttcaacttg acgatgagtc catcacttgg ttctatgaca
    58321 ccatggatcg ctattttagt gggtggcgtc gtaggtatcc tattttctct gattcacgca
    58381 gtagcaacag tcagtttgcg tgctgaccat attatcagcg ggacagtaat taatctgatg
    58441 gcacctgctt taggtgtttt cttgatcaaa gcatggtatg gtaaaggtca aactgataac
    58501 atcacgcaga atctggggta tttttctttc ccaggattag cagatattcc ggtcattgga
    58561 ccaattttct ttaaaaatac attcttacct gcttattgtg ctattttgat tgctgtccta
    58621 gcttgggtga tcatcttcaa gacgaaattc ggcttgcgcc tccgttcagt tggtgaaaat
    58681 cctcaagctg ccgacacatt aggtatcaat gtatatgcta tgcgttattc aggcgtactg
    58741 atttctggct tgttaggcgg tatgggcggt gctgtttttg ctcagtcgat ttccggtaac
    58801 ttttcagctg gtacgatcgt tggacaaggg ttcatctcca tggctgctat gatctttggt
    58861 aagtggaatc ctttaggggc aatgggtgcc tcattgttct ttggttttgc tcaaagcttg
    58921 agtatcattg gcgcacaatt accagttatt tcttcgattc cgccagtatt gctgcaaatt
    58981 gctccatatt tgctgacaat cattgtgctg gttgttttct tagggaaagc ttctggtcca
    59041 aaagcaaacg ggaagaacta tatcaaatca aaataatctt ttagcgaata aaaagggtgg
    59101 gttacgggaa gtgtttttcg tctcgccctt tttgttttaa aggcaataaa aataaaaaga
    59161 aagaaggaat aatcatgttt aaacgcgtac atctgatcgt catggattca gtaggaattg
    59221 gagaagcacc agatgccgat aagttcggtg atgttggttc tgatacatta ggacacattg
    59281 cgaaagaagc aggattgaac gttccacatt tagagcagct tggtttagga acgatccgtc
    59341 ctcttgaagg tgtggaatca gtgaaagatc atcaaggcta tgcgacgaaa ttagaagaag
    59401 tttctgtagg gaaagatacg atgacaggcc actgggaaat catgggattg aatatcaaaa
    59461 ctccatttcg tgtgtttcct aatggattcc ctgatgatct gatcaaacaa atcgaagaat
    59521 tttctggccg taaagtcgta tgtaacaaac cttatagcgg aacagctgtt attgacgatt
    59581 acggtgaaca ccaaatgaaa acaggcgatt taattgtata tacttctgcc gatcctgtcc
    59641 ttcagatcgc ggcacatgaa gaaatcattc ctttagaaga attatatcgg atttgccaat
    59701 tcgttcgtga tataacaaaa gatgatccgt atatgattgg ccgaatcatt gcacgtccgt
    59761 acgtgggtga accaggagac ttcacacgaa caagcaatcg ccatgattat gcgttgaatc
    59821 cattcggaca cacagtcctt gattctttga aagaagcagg aaaggacgtg attgctgttg
    59881 gaaagatcaa cgatattttc aacggacaag gaatcactga atctgtccgc acgaaaagca
    59941 atatggatgg cgttgaccag ctgctgaaag ttatgaaaaa cgatttttca ggaatcagct
    60001 ttaccaattt agtagatttt gatgcattat acggtcatcg tcgggatgta gtcggctatg
    60061 cgcatgcgat tgaagaattt gacttacgaa ttccagaatt gcttgaagcg atggaagaag
    60121 acgacttgct aatgattaca gccgatcacg gaaacgatcc gacgtttact ggtaccgatc
    60181 atacgcgaga atatgtaccg ttattggttt acagtaaaaa aatgaaagaa caaggggcat
    60241 tgcctcaagg atattattca gatatttcag caacgattgc tgagaacttt agtgtttctt
    60301 cgactgaaaa tggcgaaagc ttcttgaaat tattaaaata atctttttaa ataggagaaa
    60361 gcaaatgacc aagttaagcg agatgttaca agaaacgacc cagtttatcc ttgataaagg
    60421 agtaaaagaa attgattttg gtttgattct aggttcagga ttaggtgaat tagcagatga
    60481 aatcgaagat gctgtaatga tttcttatga tgagatcccg ttctttcctg tttcgacagt
    60541 agttgggcat gcaggaaagc tagtttatgg gtctctggca ggcaagaaag tgttagccat
    60601 gcaagggaga ttccactact atgaaggtca ttccatgcaa acagtgacct atccagtacg
    60661 tgtgatggcc ggactagggg cccattcagt cattgtgact aatgcatgtg gtggtgtcaa
    60721 cgaatcattt gtaccaggag atttgatgct gattaccgat catatcaatt ttactggtga
    60781 aaatcctttg atcggtccga atgaagatgc aatgggccct agatttccag atatgagtca
    60841 agcttatacg cctgactatc aagcaacagc aaaagcagca gcagaaaaac taggtttgga
    60901 tcttaaagaa ggcgtatata tgggctattc tggaccaact tatgaaacgc ctgcggagat
    60961 ccgaatgtct cggacaatag gagccgatgc agtagggatg tcgactgttc cagaagtcat
    61021 cgtagcagct cacagcggat taaaagtgtt aggaatctct tgtattacca acttagcagc
    61081 aggtatgcaa gcgaatctga gccatgaaga agttgtagaa acaactcaga gagtcaaaca
    61141 atcttttaaa gcattggtta aagaaacatt gacattattg taataggagg aacaataaaa
    61201 tgagtgtgca tattgaagcg aaagaaggcg agatcgccga taagatttta cttccgggag
    61261 atcctttgcg agctaaatat attgcggaga cttttttgga agatccagtt tgttacaatc
    61321 aggtacgcgg tatgttaggc tttaccggca aatataaagg agaacgtatt tctgttcaag
    61381 gaacaggaat gggaatgcca tcagccacca tctatgccca tgaattgatc cagtcgtatg
    61441 gtgtaaagaa attgattcga gtaggtacat gcggtgcatt gtcaaaagac gtccatgttc
    61501 gtgatttagt actagcacaa ggtgcagcga ccagttcttc gatgatcgaa aaaaatttcc
    61561 aagcctttca ttttcctcca atcagtgatt tcaatctatt actgaaagca tatgagatag
    61621 ctaaagaaaa gggctatacg gttcatgtag gaaatgttct ttcggaagat tctttctaca
    61681 aagatgattt gacagagacg ttccaattag ctgaacttgg cgttttagga gtggaaatgg
    61741 aagcagcggc tctttattac ttaggcgcga aataccatgt gcagacatta agtttgatga
    61801 ccgttagcga ccatttgatc accggtgaag aaacaacagc tgcagaacgt caatctacgt
    61861 tcaatgaaat gatcgaagta ggattggaaa cagcgatagc agaataagaa ggaaatgaaa
    61921 aagctgccct gcattaagta ataagaacat ccaagcaaaa atagctcctc atgtttttga
    61981 cttggctgga cttattattg caaatgtaag cttttgaaca ccgtttacca agaggttgtg
    62041 aatctgccgt ttaactctga gtcacaagga acaatttcaa aaaacagctt ttcatgtttg
    62101 atgaatattg tgacttgtga ccgagaagtt ggcaggagaa aaccgtttat acgaaaaaag
    62161 ggactgtgac aaatcgtgtc acagtccctt ttttatagtg ttttttatag gtagtatttt
    62221 ttagcaactg ttaagtcatc atttaattca taaacaagtg gttgacctgt tggaatttca
    62281 agatccatga tgtcttcgtc agagatacct tcgatatgtt tagctagggc gcgtaatgag
    62341 ttaccatgtg cagctactaa aacagttttg ttgtctagta atgctggagc aatttcatct
    62401 tgccagaatg gcaaagcgcg ttctaatgtc actttcaagt tttctccacc aggaatatcg
    62461 cgtttgtcta acattgcgta acgacggtca tttgctgcag aaccttcgtc agtttcttcc
    62521 attaatggag gaagagtatc gtaagaacga cgccagatgt gcacttgttc gtcgccgtat
    62581 ttttcagctg tttctttttt gttcaaacct tgtaatttac catagtgacg ttcgtttaag
    62641 cgccatgatt tgatttgtgg tacccaaagt tgatctgaat attctaagat caagttacat
    62701 gttttgattg cacgagttaa aacagaagtg taagcaacat caaattcgat tccagcttct
    62761 ttgattttgc gtccgccttc ttttgcttct tcgattcctt ctggtgctag atttacatca
    62821 gcccaaccag taaattggtt caatgcattc cattcactaa gaccgtgacg agaaaaaact
    62881 aattttggca tgtttagctc tccttttaag ataggtttat atttctgaaa ttttcagttc
    62941 tgttttattg tacaattttt tggaagagat ttccatcgaa aaattagata agattctatg
    63001 agaaaacaag tagagaatat aaaaaaacga acagtgccca cgcaactgtt cgtttttagg
    63061 tattagatgc ataattgctc gatttctttt tcaggtaaat tgatatattt ataacatgtt
    63121 ccaactgaaa caccgcattt tgaagaaata aattggatcg tttctttttt ttcataatag
    63181 agacgtcgga tttttttgat cgtttttgca tcgatttttg gtcttccgcc gatttttcct
    63241 tttttacgag cttcatctaa tccaactaat gtacgttctt tgatcaatgc gcactccatg
    63301 tttgctaggt tttccattaa ttggaaatag atttttccca tttcgcgcgt atcgattcct
    63361 tcatcaagac taatcaagtg cagcccgcga tctttgaata attgcgtgag ttctgttaat
    63421 tgccgcgtag atttgcctaa tcgatgaagt tggcaaatga ctagtgcatc gccttgttgc
    63481 atgtcagcga tcactgactc caaatgaaca gcagtagaat cattttgttc cacttcatat
    63541 gtctcatgga aaatttcttg acaaccaaag tcagataatt gatcaacctg agttgttaaa
    63601 tcaacgtcat ttatggttgt gcgtgcataa ccaataattt tcatggtgat tcctttctag
    63661 cagtgatttt ctaactggat ataacagtaa aaatgtagca taaagtacgt caaaaagata
    63721 gttttttcgc aaattttccg ctgtcttttt ttattggatg atcaagcagt ttttctttat
    63781 ttacgacgga ttgcgtcaag aatctcaacg cctttttctg agccgacaac tactttagaa
    63841 accatgttta ggaaaagccc atgttctact accccaacaa gttcatctaa ataggtagct
    63901 aagctttctg gctgttcgat gacttctaga tgcagatcaa tgatgtaatg accaccatct
    63961 gtggttaaga cttctccgtt ttcgtctgta cgcaagacgg gttggaatct tttttcttca
    64021 aaaatatgaa gtagctgttg ggagccataa gggatcactt caactgggag tgggaacttc
    64081 ccaagtttat cgactaattt agaagaatca acgatccaaa tcgtttcttt tgagtacgta
    64141 gcgactattt tctcgaaaag caaagcagcg ccgccgcctt tgatcccttg aaaatcagca
    64201 ctgatttcat cagcaccgtc tattgtcaaa tcgactacgg ggacttcatc aatactcttt
    64261 aacggtattc ctaatttttc cgcttgttct tttgtcgcat ttgaagtagt aactcctgtg
    64321 atacgtaaac cttcttcacg catacggcga ccaatctctt ctaccatata ataggcggtt
    64381 gaaccggtcc cgagacctac gatcatcccg tcctttacga atttcgcggc ttcaatgcca
    64441 gctaattgtt ttaaattcat ctttgcgtcc accttttttt agtttgagca tcgtgctttc
    64501 ctcactattt tagcgtgatt tacggtaaaa agataccatt cttttttatt ccaataaagc
    64561 tttttcaaaa aaaattgcat ctcttgttga catgtttttt cattcgtgat attctaacaa
    64621 aggtgcttta tctacaggtt tctgtgaaga aacgtgctga ctgtttatca aagcatattt
    64681 gataaggatg cagacattgc atttattttt tatcacaaaa aaagaaccac ctggatgtgt
    64741 ggaactagac gcaaatcgtg ggaaggagga aacaaggaat gcctacaatt aaccaattag
    64801 tacgtaaacc tcgtaaatca aaggtggaaa aatctaattc acctgctttg aacaaaggat
    64861 ataacagctt taagaaaact caaactaacg taaactcacc tcaaaaacgt ggggtagcaa
    64921 ctcgtgtggg tacaatgaca cctaaaaaac cgaactcagc tttacgtaaa tatgcacgtg
    64981 ttcgtttgtc aaacttaatt gaagttactg cttatatccc agggattggt cataacttac
    65041 aagaacacag tgttgtatta ttacgcggtg gtcgtgtaaa agacttacca ggggtacgtt
    65101 accatatcgt tcgcggtgct ttagatactg ctggtgttaa cgaccgtaaa caaagccgtt
    65161 ctaaatacgg aactaaaaaa cctaaagctt aattttaatt attaaacaaa tgaaacttgc
    65221 ttctactata tagaagtaat tactatgaac aatatttcgg aaggaggagt tacggatgcc
    65281 acgtaaaggt cctgttgcaa aacgtgatgt tttaccagat cctatttata actcaaaatt
    65341 agtaactcgc ttgatcaacc gtgtaatggt tgacggaaaa cgcgggattg cagctaacat
    65401 tatctataat gcctttgact tgatcaaaga atctacaggt aacgatccat tagaagtgtt
    65461 tgaacaagca atgaaaaacg ttcaacctgt tcttgaagtt aaagcacgtc gtgttggggg
    65521 ttcaaactac caagtaccag ttgaagttcg ccctgaacgc cgcgttactc taggtcttcg
    65581 ttgggtagta aactacgctc gcttgcgcgg tgaacacaca atggaagaac gcttagcaaa
    65641 agaaatcatg gatgctgcaa acaacacagg tgcttctgtt aaaaaacgtg aagacactca
    65701 taaaatggct gatgccaacc gtgcgtttgc tcactatcgc tggtaagatt ctctctgttt
    65761 ggtcggcttt tataagtcga cctcagatag tgtccttatt tttagcaact agatagtaaa
    65821 gagaggagaa acaaggagaa tgaccagaga attttcttta caaaacactc gtaatatcgg
    65881 tatcatggcc cacatcgatg ctggtaaaac gactgctact gaacgtatct tgtactatac
    65941 tggtaaaatc cataaaattg gtgaaactca tgaaggagct tcacaaatgg actggatgga
    66001 acaagaacaa gaacgtggta ttacgatcac ttctgctgct actacagcac aatggaaggg
    66061 tcaccgaatc aacatcatcg acacaccagg acacgtagac ttcacagttg aagttgaacg
    66121 ttcattgcgt gtacttgatg gtgctgtaac tgttcttgat gcacaatcag gtgttgaacc
    66181 tcaaactgaa acagtttggc gtcaagcaac aacttatggt gtacctcgta tcgtatttgc
    66241 aaacaaaatg gacaagatcg gtgctgactt cttatattca gtatcaactt tacatgaccg
    66301 tttgcaagca aacgctcacc caatccaatt accaattggt gcggaagacg acttcacagg
    66361 aattatcgac cttgtaacaa tgaaggctga aatgtataca aacgatcttg gaacagagat
    66421 cgaagaaaca gaaattcctg aagaataccg cgaattagct gaagaatggc gcgaaaaatt
    66481 ggttgaagct gtagctgaaa ctgatgaaga acttactttg aaatacttag aaggtgaaga
    66541 aatcactgaa gctgagttaa aagaaggtat ccgccgcgca acagttaacg tagaattcta
    66601 cccagtacta tgtggttcag catttaagaa caaaggggtt caattactat tggacgctgt
    66661 tcttgattac ttgccatcac cgttagatat cccagctatc aaagggattg accctaaaac
    66721 tgatgaagaa gtagaacgtc ctgcagacga cagcgctcct ttctcagctt tagcatttaa
    66781 agttatgact gacccattcg taggtcgttt aactttcttc cgtgtgtact ctggtgtttt
    66841 acaatcaggt tcatacgtac aaaacgcaac aaaaggcaaa cgtgaacgtg ttggtcgtat
    66901 cctacagatg catgccaact ctcgttcaga aatctctgaa gtttacgctg gagatatcgc
    66961 tgctgctgtt ggtttgaaag acacaactac tggggacaca ctatgtgatg aaaagagtct
    67021 agttattctt gaatccatgg aattcccaga accagttatc caagtcgcta ttgaacctaa
    67081 atcaaaagct gaccaagata aaatgggtgt ggctttacaa aaactatctg aagaagatcc
    67141 tactttccgt gctgaaacaa acgtggaaac aggcgaaaca atcatcgctg gtatgggtga
    67201 acttcactta gatatcatcg ttgaccgtat gcgtcgtgaa ttcaaagttg aagctaacgt
    67261 aggtgcgcct caagtatctt accgtgaaac tttccgtgct ggcactcaag ccgaaggtaa
    67321 gtttgtacgt cagtctggtg gtaaaggtca atacggtcac gtatggattg aatttacacc
    67381 aaatgaagaa ggtgctggtt tcgaatttga aaatgcgatc gtcggtgggg ttgttcctcg
    67441 tgaatacatt ccagctgttg aaactggttt gaaagatgct atggaaaatg gtgtcttagc
    67501 tggctatcca ttagtagata tcaaagctaa actttacgat ggttcatacc atgatgtcga
    67561 ttcaaatgaa actgccttcc gtgttgctgc ttctatggca ttacgtgcag cagctaagaa
    67621 agctaatcca gtaatccttg aaccaatcat ggctgtagaa gttgttatcc ctgaagatta
    67681 tttaggtgat gttatgggtc acgtaactgc tcgtcgtgga cgtgttgaag gtatggaagc
    67741 tcgcgctggc ggccaacaag ttgttcgtgc gatggttcca cttgctgaaa tgtttggtta
    67801 tgctacaact cttcgttctg ctactcaagg tcgtggtaca tttacaatga catttgacca
    67861 ctacgaagat gtaccaaaat cagtacaaga agaaatcatt aagaaaaacg gcggaaaagc
    67921 tgaataattt cttaaaaccg gatagtcctg caaagagatt atccaaagag actatatttt
    67981 taatatagtt tcgtgtaaaa taattatcga tgatatgggc atgtggtgta ccttgtgtac
    68041 atcatatacc tatcagacaa ataaaaaata tattgtattt ttaggaggac attttaaaat
    68101 ggcaaaagaa aaatttgacc gttctaaacc acacgttaac attggtacta tcggacacgt
    68161 tgaccatggt aaaactacat taacagctgc aatcacaact gtactatcta agaaaaacgg
    68221 cggccaagct atggcttacg atcaaatcga tggtgctcca gaagaacgcg aacgcggaat
    68281 cacaatctca acagctcacg ttgagtatga aacagataca cgccactacg ctcacgttga
    68341 ctgcccagga cacgcggatt acgttaaaaa catgatcact ggtgctgctc aaatggacgg
    68401 agctatcttg gtagtttctg ctgctgacgg cccaatgcct caaactcgtg aacacatcct
    68461 attgtctcgt caagttggtg ttccttacat cgttgtattc ttgaacaaag tagacatggt
    68521 tgatgacgaa gaattactag aattagttga aatggaagtt cgtgacctat taacagaata
    68581 cgaattccct ggtgacgatg ttcctgtagt tgctggatca gctttgaaag ctctagaagg
    68641 cgacgcttca tacgaagaaa aaattcttga attaatggct gcagttgacg aatacatccc
    68701 aactccagaa cgtgacaacg acaaaccatt catgatgcca gttgaagacg tgttctcaat
    68761 tactggacgt ggtactgttg ctacaggtcg tgttgaacgt ggacaagttc gcgttggtga
    68821 cgaagttgaa gttgttggta ttgctgaaga aacttcaaaa acaacagtta ctggtgttga
    68881 aatgttccgt aaattgttag actacgctga agctggagac aacattggtg ctttactacg
    68941 tggtgttgca cgtgaagaca tccaacgtgg acaagtttta gctaaaccag gtacaatcac
    69001 acctcataca aaattctctg cagaagtata cgtgttgaca aaagaagaag gtggacgtca
    69061 tactccattc ttcactaact accgtccaca attctacttc cgtacaactg acgtaacagg
    69121 tgttgttgaa ttaccagaag gaactgaaat ggttatgcct ggcgacaacg taacaatgga
    69181 agttgaatta atccacccaa tcgctatcga aaacggtact aaattctcaa tccgtgaagg
    69241 cggacgtaca gttggtgccg gtgtcgttac agaaatcaaa gcttaattca cttaagtttt
    69301 aaagtaaagc aacatttatt cggacgtaat cgctcggaac gaaggtctta ggatctttgt
    69361 tccgagcttt tttatttatt gcaggatcag gaaaaaagac aggagtatta taaatagaag
    69421 aaacacgaaa taaaagagtc agtattttct aaatgaaact ttatgaaaac aaaatgtttt
    69481 ttacaagaaa aagccttgct ttgtgagttt atatatatta taatgacaaa ggtgcgttta
    69541 ctttagtgaa ttgcacagac gtaatcagaa tgatttcgtc ggctaagaaa cgagaggttg
    69601 cgacacgccc ggacgctttg ccacgaacga gcgtgacgga aattttcgtg gagctatgtc
    69661 tacttttcaa aataggcgaa ggagggaaca agatggcaaa acaaaaaatt cgtatccgtt
    69721 taaaagcgta tgaacaccgt attttagatc aatcagcgga taaaatcgtg gaaactgcaa
    69781 aaagaactgg agctagcgtg tctggtccaa ttccattacc aactgaacgc agtctttaca
    69841 caatcatccg tgcgactcat aaatacaaag attcacgcga acaattcgaa atgcgtacac
    69901 acaaacgtct tatcgacatt gtgaacccaa caccaaaaac tgttgatgct ttgatgaagc
    69961 tagacttacc atctggtgta aacatcgaaa tcaaattata aaactaaaac aaaaataatg
    70021 gaggtgtact catgaccaaa ggaatcttag ggaaaaaagt gggaatgaca caaatcttca
    70081 ctgaatctgg tgaattaatt ccagtaactg ttgtagaagc tactccaaac gtagtactac
    70141 aagtgaaaac aatggaaacg gatggctacg aagctattca agttggttac caagatatgc
    70201 gtgaagtttt atcaaacaaa cctgcgaaag gtcatgttgc aaaagcaaac acggctccta
    70261 agcgcttcat tcgtgaattc aaaaatgttg agctaggaga atatgaagta ggaaaagaaa
    70321 ttacagtaga tgttttccaa gcaggagaca ttattgatgt aacaggtact acgaaaggta
    70381 aaggattcca aggggttatc aaacgccacg gacaaagccg cggaccaatg gctcacggtt
    70441 ctcgttacca tcgtcgtcct gggtcaatgg gtccagttgc acctaaccgt gtatttaaaa
    70501 acaaacgtct tgctggccgt atgggtggca accgcgtaac tatccaaaac ttggaagttg
    70561 tacgtgtcga tgcagaaaga aatgttatct tgatcaaagg taatgttcct ggagctaaaa
    70621 aatctttaat tactatcaaa tcagctgtga aagctaaata attgtaggaa gaaaggagga
    70681 actaaggaat gccgaatgta gcattattca aacaagatgg aagccaaaac ggtgaaatca
    70741 cacttaacga agaaatcttc ggaattgaac ctaatgaaag tgttgtttac gatgcaatcg
    70801 taatgcaacg tgcgtcatta agacaaggaa cacacgcggt taaaaaccgt agcgctgttc
    70861 gcggcggcgg acgtaaacca tggcgtcaaa aaggaacagg tcgtgcacgt caaggatcta
    70921 tccgctcacc acaatggcgt ggaggtggcg tagtctttgg accaacacca cgttcttaca
    70981 gctacaaact tcctaaaaaa gttcgtcgtt tagcaatgaa atctgtattg tcagaaaaag
    71041 ttgctgaaaa caacttggta gcaatcgaag gcttgaactt cgacgcacca aaaacaaaag
    71101 aattcaaaca agttcttgct aacttatcta tcgactctaa agtattggtt gttctagaaa
    71161 aaggtaacga ctttgctgct ttatcagcac gtaacttacc aaacgtttct gtagtaactt
    71221 ctgataatgt gagtgtgcta gacgttgtat caaatacaaa agtgttagca acacaaactg
    71281 ctcttactca aattgaggag gtgcttgcat aatgaactta ctagacgtaa tcaaacgccc
    71341 agtgatcact gaaaaatcaa tgcttgccat ggatgacaag aaatacactt ttgaagtgga
    71401 cactcgcgca aacaaaactt tagtaaaaca agcggtagaa gctgcttttg acgttaaagt
    71461 caaaaacgtg aacatcgtga acgtacgtgc gaaattcaaa cgtatgggca aatatgcagg
    71521 atatacaaaa aaacgtcgca aagcgatcgt tacattgact gaagattcaa aagaaatcca
    71581 attattcgaa gctgctgaat aagcactaat agataactca ttaaattagg agggaaaaac
    71641 gtggcgatta aaaagtacaa acctacctca aatggtcgtc gtaacatgac aggttctgat
    71701 tttgctgaaa tcacaacatc aacacctgaa aaatcattgt tacaaccatt gaaaaaccat
    71761 gctggacgta acaacaacgg ccgcatcact gtacgtcatc aaggtggcgg acacaaacgc
    71821 caatatcgcg tgatcgactt caaacgtaac aaagataacg ttgttgcaac tgttcaaacg
    71881 atcgaatatg atccaaaccg ttcagctaac atcgcgttag tacactatga agatggagtg
    71941 aaagcttaca tcttagcacc taaaggacta gaagtgggta tgaaacttgt ttcaggtcca
    72001 gaagcagata tcaaagtcgg aaatgctctt ccattagaaa acattccagt tggtactgtt
    72061 atccacaaca ttgaaatgaa acctggtaaa ggtggacaat tgatccgttc tgctggtaca
    72121 agtgctcaag tacttggtaa agaaggcaaa tacgtattga tccgcttgaa ctctggtgaa
    72181 gttcgtatga tcttagctgc atgccgtgct acaatcggtt ctgtaggtaa cgaacaacac
    72241 gaattaatca acatcggtaa agctggacgc tctcgttgga tgcgtaaacg cccaactgta
    72301 cgtggtagcg taatgaaccc taatgatcac ccacacggtg gtggtgaagg taaagctcct
    72361 atcggacgta aagcaccagt ttctccttgg ggtcaaccag ctcttggtta caaaactcgt
    72421 aacaaaaaag ctaaatcaga caaacttatt gttcgtcgtc gcaaaaataa ataattactt
    72481 tgaccatgtg tcgcacattt tgagaggagg ttcaccatgg gtcgtagttt aaagaaagga
    72541 ccttttgtcg atgagcattt aatgaaaaaa gttgaagctc aagcaggggt tgaaaagaaa
    72601 aaagttatca aaacttggtc acgccgttca acagttttcc caagttttgt tggatatacc
    72661 atcgcagttt atgatggacg taaacatgta ccagtttaca tccaagaaga catggtagga
    72721 cacaaattag gtgaatttgc acctacaaga acttatcgtg gacatgctgc tgacgataag
    72781 aaaacgaaac gctaatttga ggggaggata aatccaaatg gcagaacaaa tcacatcagc
    72841 taaagcaact gcaaaaacag ttcgcacttc acctcgaaaa gcccgtttag taattgacct
    72901 tatcagaggt aaaagtgtcg cagatgcaat ttcaatcttg aaattcacac caaacaaatc
    72961 tgctggaatc attgaaaaag tattgatgtc agcagttgcg aatgcagaaa ataactttga
    73021 cttagatgtt gaaaacttgg tagtaagcga agcttttgtt aacgaaggac ctacaatgaa
    73081 acgtttccgt cctcgtgcaa aaggatcagc ttctccaatc aacaaacgta caagtcatat
    73141 cacggtagtc gtatcagaaa aataaggagg gataatcagt gggtcaaaaa gtacatccaa
    73201 ttggaatgcg tgtaggcatc atccgcgact gggatgctaa atggtatgct gaaaaagagt
    73261 atgctgagtt cttacacgaa gatttaagaa tccgtaaatt tatttcaact aaacttgctg
    73321 atgctgctgt atcaacagtt gaaatcgaac gcgcaacagg tcgcgtaaac atttcaattc
    73381 acacagctaa accaggtatg gttattggta aaggcggttc tgaagtcgaa agcctaagaa
    73441 aagaattaaa caaattaact ggtaaaagaa tccatatcaa tatcgtggaa atcaaaaaac
    73501 cagatctaga tgcaaaatta gtaggtgaag gaatcgcacg tcaattagaa aaccgtgttg
    73561 ctttccgtcg cgctcaaaaa caagccatcc aacgctcaat gcgtgctggc gctcaaggga
    73621 tcaaaactca agtatctggt cgtctaaacg gagcagatat cgctcgttca gaaggatact
    73681 cagaaggaac tgttcctctt catactttac gtgcggacat tgattacgca tgggaagaag
    73741 cagatacaac atacggaaaa ctaggagtta aagtgtggat ctatcgtgga gaaattcttc
    73801 caactaaaaa aaacactgag aaaggaggga aataatcatg ttagtaccta aacgtgtaaa
    73861 acaccgtcgt gaattccgcg gaaaaatgcg cggggaagct aaaggcggaa aagaagtagc
    73921 attcggtgaa tacggtttgc aagctgttga ttcacattgg atcacaaacc gccaaatcga
    73981 agctgctcgt atcgcaatga ctcgttacat gaaacgtggt gggaaagtat ggattaaaat
    74041 tttccctcac aaatcttata ctgccaaagc aattggggta cgtatgggtt ctggtaaagg
    74101 ggcacctgaa ggatgggttg caccagtaaa acgtggtaaa atcatgtttg aaatcgcagg
    74161 cgttcctgaa gaagtagctc gtgaagcgtt acgtctagct tctcacaaat taccaatgaa
    74221 aactaagatc gtaaaacgtg aggaaatggg tggtgaatcg aatgaaggtt aaagaaatca
    74281 gagaattaac cactgccgaa atgcttgatc aagaaaaaca attaaaagaa gaattgttta
    74341 atcttagatt ccaattagca acaggtcaat tagaaaacac tgcacgtatt aaagaagtac
    74401 gtaaatcgat tgcacgcatc aaaactgtgt tgcgtgaaca agctaagtaa ttatggaagg
    74461 aggccattaa gcgtatgact gaaggaagaa atcaacgcaa agtttatcaa ggtcgcgtgg
    74521 tttcagacaa aatggataaa acaattaccg ttgctgtcga aacaaagaaa aaccacccta
    74581 tctatggcaa acgcatgaac tattctaaga aatacaaagt gcatgatgaa aacaacacag
    74641 caaaagttgg cgacatcgtg agaattatgg aaactcgtcc attatcagct acaaaacgtt
    74701 tccgtttgtt ggaagtagtc gaagaagcag ttattatcta ataacacgag attttcctat
    74761 atagattgaa tgcaaaatct gaaaggagga tacacaacgt gatccaagca gaaagtcgtt
    74821 taaaagtcgc tgataactca ggcgcacgtg aaattttgac gatcaaagtc cttggtggat
    74881 ctggtcgtaa aactgctaac atcggtgatg tgatcgttgc tacggttaaa caagcaacgc
    74941 caggtggggt tgtcaaaaaa ggtgacgtcg ttaaagccgt tatcgttcga acaaaatcag
    75001 gagctcgtcg tacagacggt tcatatatta aatttgatga aaatgctgca gtgattattc
    75061 gtgatgataa aagccctcgt ggaacacgta tctttgggcc agttgcacgt gaactacgtg
    75121 aaaacaactt catgaagatc gtttcactag caccagaagt tttataatct tgagacactt
    75181 atcaaaggag gtgcgaaaca gttatgtttg ttaaaaaagg cgataaagtt aaagttatca
    75241 ctggaaaaga caaaaacaaa gaaggcgttg tactagcagc gttcccgaaa aaagataaag
    75301 ttattgtcga aggtgtaaat gtcgttaaaa aacaccaaaa accttctcaa gctgctcctc
    75361 aaggtggaat tgttgagatg gaagcgccaa ttcatgtttc taacgtgatg gtggttgact
    75421 ctactggtgt agctggccga gttggctaca aagaagtaga cggcaaaaaa gtccgtgttt
    75481 ctaaaaaaac cggtgaagtt ttagataaat aaaagtttaa ggaaggaggg tcttactgaa
    75541 tgaaccgcct aaaagaaaaa tatcttaaag aagtaactcc atcattgatg gaaaaattta
    75601 actatagctc tgttatgcaa acacctaaag ttgaaaaaat cgtcatcaac atgggtgttg
    75661 gtgatgcggt atcaaacgct aaaaacttag ataaagcagt tgaagaatta actttgatca
    75721 ctggtcaaaa accaatgatt acaaaagcta aaaaatcaat cgctggattc cgtttacgtg
    75781 aaggaatgcc aattggtgca aaagttacat tgcgcggaga aagaatgtac gaattcttag
    75841 acaaattggt ttctgtttca ctaccacgtg tacgtgactt ccatggtgta agtaaaaaag
    75901 cctttgacgg tcgtggaaac tatactttag gtattaaaga acaattgatc ttcccagaag
    75961 tagattatga tttagtagat aaagtacgtg gtatggacat cgttattgta acaacagcaa
    76021 actctgatga agaatctcgt gagttgttga cacaattagg catgccattc caaaaataat
    76081 taaaggaggc gaactacgtg gctaaaaaat caatgattgc taaaaacaaa cgtcctgcaa
    76141 aattttcaac acaagcttat actcgttgcg aacgttgcgg acgtccacat tcagtttatc
    76201 gtaagttcca tctttgccgt atttgcttcc gcgaacttgc ctataaaggt caaattcccg
    76261 gcgtgaagaa agctagctgg taaaaagtaa attcgcataa gaaggaggta aaagatcaat
    76321 ggtcatgaca gatccaattg cagattttct aactcgtatc cgtaacgcaa acatggttaa
    76381 acatgagtct ttagaagtac ctgcatcaaa aattaaacgt gacatcgctg aaatcttgaa
    76441 acgtgaaggt ttcgttcgtg atgttgaata catcgaagat gacaaacaag gcgtgatccg
    76501 tgttttccta aaatttggta aaaacggcga acgtgttatc acaaacttaa aacgtatctc
    76561 taaaccaggt ttgcgtgctt atgtcaaagc tgacgaagta ccaaaagtat tgaacggttt
    76621 aggtatcgca atcatttcga cttctgaagg tgttgtcact gataaagaag ctcgtgaaaa
    76681 aaatatcggc ggcgaagtta tcgcttacgt atggtaatca agtagaatct acaaggaggt
    76741 gtctttaagt gagccgtatc ggaaataaag tcgttgttat ccctgaagga gtaacagtta
    76801 ctcaagacgg aaacaacgtt acagttaaag gaccaaaagg tgaattgact cgtacttttt
    76861 cagcagatat caaaatgaat atcgaaggaa acgaagtaac tttcactcgt ccaaacgaca
    76921 gtaaagaaat gaaaacaatt catggaacaa ctcgtgcgaa cttcaacaac atggttgtag
    76981 gtgtcagcga aggtttccaa aaagcgttag aactaatcgg ggttgggtac cgtgcacaat
    77041 tacaaggatc aaaacttgta ttgaacgttg gttactcaca tccagtagaa atcacaccac
    77101 cagctggagt aactgttgaa gtaccagcta acacacaagt aattgttaaa ggtgcgaaca
    77161 aagaagaagt tggcgaatta gctgcaaaca ttcgtggaac tcgtcctcca gaaccttata
    77221 aaggtaaagg tattcgttat gttggcgaat atgtacgccg taaagaaggt aaaactggta
    77281 aataatcgtt tagaaaaatt gcatagaagc cagcagcagg aaaaaggcat ttggctgaag
    77341 tttaccttca aaaaaataca tttttacttg tgccgcagct tctatttttc gaaacgtatt
    77401 tactgcttga aatagtagta caataaaaat aaagaggtga caattgtgat tacaaaacca
    77461 gataagaaca aaacacgtca aaagagacat cgtcgtgtac gtaacaatat ctctggtact
    77521 gctgagcgcc cacgcttgaa cgtttttcgt tctaacaaaa acatctacgc tcaaattatt
    77581 gatgacgtag cgggtgtaac gctagcaagt gcctctacct tggataaaga aatttcaggt
    77641 ggaacaaaaa cagaagcagc acaagctgtc ggtaaactag ttgctgaacg tgcagctgaa
    77701 aaaggcatta aagtagtagt ctttgaccgt ggtggatacc tttaccatgg ccgtgtgcaa
    77761 gctttagctg aagctgctcg cgaaaatgga ctagaatttt agaagaagga ggaataccat
    77821 tcatggttta tatcgatcca aaacatttgg aattagaaga ccgcgttgtt gcaattaacc
    77881 gtgtaacaaa agttgttaaa ggtggacgtc gtctacgttt tgctgcttta gttgttgtcg
    77941 gtgacaaaaa cggacacgta gggtttggta caggtaaagc tcaagaagtt cctgaagcaa
    78001 tccgtaaagc aattgaagat gcgaagaaaa acttagttga agtacctatg gttggttcaa
    78061 caatcccaca cgaagtaatc ggtgctttta gtggtggtag aatcctaatg aaacctgctg
    78121 tagaaggttc tggggttgct gctggtggac cagttcgtgc cgtattagaa ttagctgggg
    78181 tagcagatat cacttctaaa tctttaggct caaatacacc aatcaacgtt gttcgcgcaa
    78241 cagttgaagg tttgaaacaa ttgaaacgtg cagaagaagt tgcagaactt cgcggcaaat
    78301 cagttgaaga attaatcgga taaggaggac aacaataatg gctgaattaa aagttacttt
    78361 aaaacgcagt attatcggac gtcctcaaaa ccaacgcgat acagttaaag cgttaggtct
    78421 tacaaagatt aacagttcag tggttaagcc ttctaatgaa gcaattaaag gcatgatcaa
    78481 tactgtttct cacctagtgg acgttgaaga aatttaattt agaaaaatca taactagaac
    78541 attaaggagg tgccaaacct atgaaacttc atgaattaaa acctgctgaa ggttcacgcc
    78601 aagtacgtaa ccgtgttggt cgtggtactt catctggtaa cggaaaaact gccggacgcg
    78661 gacaaaaagg acaaaaagct cgttcaggcg gcggtgtgcg tttaggattt gaagggggac
    78721 aaacaccatt gttccgtcgt ttaccaaaac gaggattcac aaacgtgaac cgtaaagact
    78781 atgcagtcat taatcttgat gtattgaacc gctttgaaga cggagctgaa gtaactcctg
    78841 ttgctttagt tgaagctgga atcgtgaaaa acgaaaaagc tggaatcaaa gtattagcta
    78901 atggggaact aaacaagaaa ttaactgtga aagcagcaaa attctctaaa gcagctcaag
    78961 aagcaattga agcggctggt ggatcaatcg aggtgatcta atgtttagac tattaaaaga
    79021 tgcttttaaa gtaaaagaca ttagatcgaa aatcctattt actgtatttg tgttgtttgt
    79081 atttcgccta ggtgcacata ttactgtacc aggcgtaaat gctgcttcat tgcaaaacct
    79141 ttcttctttg ccatttttca acatgttgaa cttggtaagt ggtagtgcga tgcaaaactt
    79201 ttccattttc tcaatggggg tttcaccata cattactgct tctatcgtca ttcagcttct
    79261 tcaaatggac atcgtgcctc gatttgttga atggtcgaaa caaggggaag ttggtcgtaa
    79321 aaaactgaat caagcgactc gatatctgac attagtattg gcatttatcc aatctgtcgg
    79381 tatcactgct ggattcaatc aatacgctca attaggtttc gtgaataatc ctaacgtagc
    79441 aacttacgta acgatcggtt tgattctaac agccggtacg atgtttgtta cttggctagg
    79501 agaacagatc acagaaaaag gtatcggaaa tggcgtatca atgatcattt ttgcaggtat
    79561 catttctaga ttgcctgaaa gtatcagaga aatttatgaa gattacttta taaatattga
    79621 tccttcagac atatggaaat ctgctatttt tgtggccatt ttactagtag cggtcttgtt
    79681 gatcgtgaca ttcgtaacat ttttccaaca agctgaacgc aagatcccga tccaatatac
    79741 gaaacgcgta gctggcgcgc ctacaagcag ctatctgccg ttgaaagtaa atgcagctgg
    79801 agttattcca gttatctttg ctagttcgtt tattgctacg ccaaatgctg ttttacaagc
    79861 attgagcggt agattcggtt ctgaaaactg gtacaaagtg atgatggaga tctttaacta
    79921 taatacagca cctggtgcta cgatttatac gattctgatc gttgccttca ctttcttcta
    79981 cgcctttgtt caagtaaacc ctgagaaatt agcggaaaac ttgcaaaagc aaggaagtta
    80041 tataccaagc gtacgccctg gtaaggggac tgaagagtat gtctcaagat tactgatgcg
    80101 tttaagtaca gttggtgcag tcttccttgg tttagtggca ttgcttccaa tcattgctca
    80161 aatggtttgg gatcttccac aatcaatcgg attaggcggt acaagcttac tgatcgttat
    80221 cggtgtagct ttagaaactg cgaaacagtt agaaggctta atgctgaaac gtaaatatgt
    80281 tggattcatt gagtaagaaa gcgtgttgag gtttcccctc aacacacgtt ctcacacatt
    80341 tattaggagg gaaaacaatg aacctcattt taatggggct gccaggcgca ggtaaaggaa
    80401 cacaagctga aaaaatcatt gatgcttatg gtattccgca catctcaaca ggagatatgt
    80461 tccgcgcagc gatgcaaaac gaaacagctc ttggcctgga agcaaagtct tatatggata
    80521 aaggtgcatt agttcctgat gaagtaacaa atggaatcgt gaaagagcga ctagctgaac
    80581 cagatacaga gaaaggcttc ttattagatg gctttccacg tacgctagat caagcaaaag
    80641 ctctggacgc aatgctaaaa gatttgaata aaaaaattga tgctgtcatc gatatccatg
    80701 ttggtgaaga aatcttggtc gaacgtctag ccggccgatt tatctgcagc aattgtggag
    80761 caacttacca taaaattttc aaccctacaa aagttgaaga cacatgtgat cgttgcggtg
    80821 ggcatgaatt ctatcaaaga gaagatgata aacctgagac ggttaaaaat cgtctggcta
    80881 tcaacatcaa aaatagtgaa ccaatcttgg cttattataa agaacaaggt ttgctaaaca
    80941 ctattgatgg tgctcgtgaa atcgatgctg tatttgcaga tgtacaaaaa atcattgaaa
    81001 aatagcacga ttgcccgtag ccgacttgca ttatgcagtt actcttgtca agttcaataa
    81061 attatgatag aatatgtcag tccgacttct agagaatcct ctagatgatc cattttgaga
    81121 attttgaggt gggaactgca gtttccgcca ttttatcgtg tgaaaatgcg aaacaagtac
    81181 aaggaggtac tgtgcgtggc aaaagaagat atgattgaag tcgaaggtac agtcgtcgaa
    81241 actttgccga atgcaatgtt taaagttgaa ctagaaaacg gacaccaagt tcttgctact
    81301 gtttcaggta aaattcgtat gcattacatc cgtatcttac ctggtgacaa agtaacggtt
    81361 gaattgtctc catacgatct taaccgtggc cgtattactt atcgctttaa ataattgtac
    81421 tccgtaaaac tacaggaggt attatcatga aagtaagacc atcagtaaaa ccaatgtgcg
    81481 aacattgtaa agtaattcgt cgtaaaggac gcgttatggt gatttgccca gcaaatccaa
    81541 aacataaaca acgtcaagga taattggagg tgctataaat atggctcgta ttgcaggagt
    81601 agatattcct cgtgataaac gtgtagtagt ttcacttact tatatctatg gtattggtaa
    81661 cactactgct aaaaagatct tagctgatgc tggtgtatct gaagatgttc gcgttcgtga
    81721 attaacgaat gaacaaacag atgctatccg tgcagaagta gataaattaa aagttgaagg
    81781 ggatcttcgt cgtgaagtga acttgaacat caaacgtttg atggaaatcg gttcttaccg
    81841 tggaatccgt catcgccgtg gattgcctgt tcgtggacaa aacacgaaaa acaatgcacg
    81901 tactcgtaaa ggcccagctc gcgctatcgc tggcaagaaa aaataattct taagtgaagg
    81961 aggttaaaac ttcatggcag caaaaaaagt gaatcgtaaa cgccgtgtga agaagaatat
    82021 agaagcaggt attgcacata tccattctac attcaataac actatcatca tgatcactga
    82081 tactcacgga aatgcattag cgtggtcatc agctggttca ttaggtttca aaggaagcaa
    82141 aaaatcaact ccttttgccg ctcaaatggc agcagaaact gctacaaaag cagcaatgga
    82201 acacggatta aagactgttg aagtaacagt taaaggacct ggttctggac gtgaagcagc
    82261 aattcgttca ttacaagcta caggtttaga agtgactgca attcgtgacg tgactccagt
    82321 tcctcataat ggtgcccgcc ctccaaaacg ccgtcgtgtt taatgagtgc cgctcatttt
    82381 gagttttatc agtaacgtca ttgaacaaga cacttttcgt tttgaaaggg gtaaagataa
    82441 gaatgattga gttcgaaaaa ccaagaatcg caaaaattga tgaagaaaaa gattatggca
    82501 agttcatcgt tgaacctctt gaaagagggt atgggactac tttagggaat tccctacgtc
    82561 gtattttatt atcttctttg ccaggtgctg ccatcactaa tattcaaatc gatggtgtac
    82621 tgcatgaatt ctctaccatc aaaggtgtta gagaagacgt cactcaaatc atcttgaaca
    82681 ttaaaggctt agcgctgaaa atgtatggcc aagaagaaaa aacccttgaa atcgatatta
    82741 ccggacctgc tacagtaact gctggcgata ttatcgttga tagtgatgta gaaattctta
    82801 acaaagatat gtacatctgt agtgtctctg aaggagctac gttccatgct cgcttaacag
    82861 tgaaacctgg acgtggttat gttcaagcag atgaaaataa aaaagaagat atgccaattg
    82921 gcgttcttcc agtcgattca atctacacac ctgttcgtcg tgtgaactat caagttgaga
    82981 acacacgtgt tggacaccgt gaagattttg ataaattgac gatggagatt tggacagatg
    83041 gttcaattga accgttggaa gcaatgagtt tgtctgcgaa aatcatgact gaacatttgg
    83101 atatttttgt gaacttaact gatgaagcaa aaaatgctga aatcatggtc gaaaaagaag
    83161 aaacacaaaa agaaaaaatg ttggaaatga cgatcgaaga gttagacttg tctgttcgct
    83221 cttacaattg tttgaaacgt gcaggaatca atactgtaca agaacttaca aataaatctg
    83281 agcctgaaat gatcaaagtc cgtaacttag gacgtaaatc attagaagaa gttaaagcaa
    83341 aattgcacga cttaggttta ggtttacgta aagaagatta aaacctttta cgaaggaggg
    83401 aaaccaacgt gagttatcgt aaactaggac gcacatctag ccaacgtaaa gcgatgttgc
    83461 gtgatttaac aactgattta ttaatcaacg aacgtattgt gactactgaa gctcgtgcga
    83521 aagaagttcg ctcaactgca gaaaaaatga tcactttagg taaacgcgga gatttgcatg
    83581 cacgtcgtca agctgcggct ttcgtacgta atgaagttgc cagcgtgcgt gaagaaaatg
    83641 aggacatcgt cattgaatca gctttacaaa aactgttcaa cgatattgca cctcgttacg
    83701 cagaacgtca aggcggatac acacgcattt tgaagacaga accaagacgc ggtgatggtg
    83761 caccaatggt tgtcttagaa cttgtctaaa atcacactaa taacatcact ttatagatga
    83821 ttcttttaga gtgttatgat gttggaagat atttttccga gtctagctca gcgctgctcc
    83881 tcaagcgaat atcgcttggg gagtaagcac tcatcataaa gtgttttttt ataccttcaa
    83941 ttacctgtca tgacagatat gtttgaaggc ataaaaaaac attttaagcg agaaataatt
    84001 gaaaattttg tctttttttg atagtgttag taggaacttt aggatgaaga aggagtaatc
    84061 atggagccaa ttattgaatt agaaaagata aattataaat accagccaga tgatcttcgc
    84121 cccgcattga aagatgtctc ctttacaatt gataaaggtg agtggatcgc tattattgga
    84181 cacaatggtt caggaaaatc gacacttgcc aaaacgatca atggattgct tctgccagaa
    84241 tcagggatcg tgaaagtggg aaatcaaata ttggatgaag aaaatatttg gacgatccga
    84301 cagatggttg gaatggtttt tcaaaatcca gacaatcagt ttgttggttc gacagtagaa
    84361 gatgacgttg cttttggtct tgaaaatcaa gggattccca gagaagaaat gctagtgcgt
    84421 gtgaaagatg cacttgagaa agttagaatg gctgagtttg cttcacgcga gcctgctcgt
    84481 ttatcaggag ggcaaaaaca acgtgttgcc attgccggcg tggttgcctt acgacctgat
    84541 atcattattt tagatgaagc aaccagtatg ctagatccag aaggtcgtga agaagtgatc
    84601 tctacgatca agaagatcaa agaagaaagt caattgaccg ttatatctat cactcatgat
    84661 attgatgaag cagctaatgc caatcggata ctagtgatga gacaaggcga acttgtccgt
    84721 gaaggtacac caaaagaaat tttttctgct ggtcctgaac tgatcgactt aggtctagat
    84781 ctaccatttc ctgaaaaact gaagagcgca ctgaaagaac gtggggttga tgtgcccagt
    84841 gaatatatga cagaagaaag gatggtggat tggttatgga catccgtttt gaacaagtag
    84901 acttcactta tcagccgaat acaccatttg aacaaagagc cttatttgac atcaatatga
    84961 cgatcaaaga aaacagttat actgcattag tagggcatac cggaagcggg aaatcaacct
    85021 tacttcagca tttgaatgcg cttgttaagc caacaagcgg tacagtccat attggagaac
    85081 gagatattca gccggatacg gataataaga atttaaagcc catccgaaaa aaagttggca
    85141 tcgtctttca gtttccagaa gcacagctat tcgaagaaac ggtagcaaaa gacattgctt
    85201 tcggtcctaa aaactttggt gtcagcgaag aagaagcact agtcctagca aaagaaacat
    85261 tagaacaagt tgggctggat gaaagctatt tggaacgttc gccatttgaa ctttcaggag
    85321 ggcaaatgcg tagagtagca atcgctggtg tgcttgccat gaggccggaa gtacttgtat
    85381 tggatgaacc aacggcagga cttgatcccc agggcagaaa agaaatgatg gagatgtttt
    85441 ggcgtcttca taaggaacag cagatcacta ttgtattagt tacgcattta atggatgatg
    85501 tagccaattt tgcagattat gtctatgtgt tggaaaaggg acgaatcgtt aatagcgggg
    85561 agccgcaaga ggtctttcaa aacattgaat ggctaaaaga aaaacaacta ggcgtaccta
    85621 ctgccaccga gtttgcagaa gaattaatgg ctaaagggat gaactttgct accttgcctt
    85681 tgacagctga agaattagca gacgctatcg tccattatgc tggaggaaat gcccatgatg
    85741 aataaattga tatttggtcg ttatatgcct ggagattcct tgatccatcg attggatccc
    85801 cgagctaaat tattagccag tttttatttt atcggaatca tttttttagc aaataattgg
    85861 gaaacgtacc ttttgttggc atttttcaca ttactttctg tcttcttgtc aaaagtagat
    85921 ttgaaatttt ttatccgagg cattcgccca ttgatctggc taatcctctt cactgtagct
    85981 ttgcaggtct tgtttacaag tggcggagaa acttattgga gttggggtgt tttccatatc
    86041 actgaatttg gtgtgatgaa cggcctcttt attttctgtc gattcgtgtt gatcattttt
    86101 atgtcgactc tactcacgct tactacaccg cctttggaat tatcggatgc gattgagtac
    86161 ttgcttcgtc cattgaaagc cgtacggttc ccagtccacg aaatttcatt gatgctgtcg
    86221 attgctcttc gttttgtgcc tacattgatg gacgaaacag aaaagatcat gaacgctcag
    86281 cgcgctcgcg gtgttgattt tggtgaagga aacctagttc agaaaatgaa agcagtcatt
    86341 cctttgctga ttcctttatt tgtcagcagc ttcaaccgtg cggaagatct tgcgactgct
    86401 atggaagcaa gaggctacaa aggtggggaa ggaagaacga aatatcgtgt attgcattgg
    86461 cataatagag acacgatggt tatcattgct tttgtattat taactgtgct actgatcttt
    86521 ttaagaggat aaacgttcag tgggccgcta tctattcttt tcttgctgat ctgtttgcaa
    86581 gaaaggatgc aagcagtgta tgacaagaaa tattagggtc tgtggcagac taaggtcaca
    86641 ggcctctttc ttttatctga aaagaaagaa cgaaaaaatt ttatagaaaa atcattattc
    86701 cagataacct tttaggaaca aaggagagaa aaacatggta cgttacaaag caatcattgc
    86761 gtacgacgga acaaatttta atggattcca aaaacagcca aatgggcgta ctgttcaaga
    86821 agaagtagaa aaaacattac aaaaaatggc aaatggaaaa gaaatcacgg tctttggttc
    86881 gggaaggaca gatgccggag tacatgcgat cgggcaagta atccattttg attatccaga
    86941 ggagcgtccg ttagaacgaa tgcgtttcgc gctagatacc cagtcaccag aggatattgc
    87001 tgtaagacaa gtagaaattg tttcagaaga cttccacgca agatatttgg tcaaagaaaa
    87061 gacgtatcaa tttcgagtgg atatagggaa accacgcagt ccttttcgac gccattatgc
    87121 aagttatttt ccttacccac ttgatttgga aaaaattcag cgggcgcttc ctgatttatt
    87181 agggactcac gatttcacct ctttttgtgc atcaggaagc tcgatagagg ataaaatacg
    87241 tacgatctac gaggctaaga tggaggtaaa tgaaacaaaa gacgaattgt tgtttacatt
    87301 taggggaaat ggcttcttgt ataagatgat acggatctta gtaggaacgc ttttgaaaat
    87361 tggcaatggc cgattgcctg aagatagtat cccagatatt attgcgaaaa aagatcgtaa
    87421 tgcagcagga cctacagcac accctgaagg actttatttg tatgaagtga aatatgaata
    87481 agaggttatg atagaagtgg tcagcttcaa gaaataagaa ggtatttacg aaaattactt
    87541 ctcaaaattt tgcaaaattt cggcttattt tcgaagaagc tacttctgga acaccgttta
    87601 agaaagaggt cgtgagcctg tcatttaact ctgagtcaca aagagcaatt tcaaaaaaca
    87661 gcttctcatc tttgatgaat atcgtgactt gtggacgagg cgttgataag tgaatcatct
    87721 ttttgttacc tattttttct gtaaccaaaa aacgatggaa cgttgttttg attgcccttg
    87781 gtgcggacag tctgaacagg ttccatcgtt tctttatttt ctacgtcttc ttctgtgtgt
    87841 gtttcgtggt tgttcttcta ttgctggatc aatcgtgcca taaaaggggc gattgcggtc
    87901 aaggtaccag acataaagaa taaacaatgc aatacatagg acaaaaagga ttgcacctat
    87961 cgtaaatcct tcaggcaagt aactgaaagt gacagtatgt ttacctgctg gaacttttat
    88021 catcaagaat gcgtctttga aattgctgac cttgactggt ttcccatcaa ttttagcttt
    88081 ccatcctttg tcataaggaa tagttgtcac caacatggaa tcttcagcgg tatttacagt
    88141 cccagaagct tttctgccag tggcttccat ttcgacacct ttttctttga tctgattcat
    88201 tgcggcttga taggctacaa catctagacc aacgacttta ggctccatga aacttatttc
    88261 tttcgtccca taaaaacttg cagtaaatgt cactgttgtc tcttgaggat aatagccaag
    88321 atcatagtat tgaccagaaa tgttgatttg cgtttttcgc gatgtaccat tgacagtgat
    88381 cgtcgcacta gaactttcta attgcgcaaa attggttggg aacaaactta agtaagcttg
    88441 tgtatttgca ggcacatgga cttcccaagt gagctctttt ggctggttat ctgctgtttc
    88501 tgagtaagtg acgccagtcg ttgtctttgt gatcgtcgta ttcaactgat tcttcaaagt
    88561 aggtgtatag aatgtaaaat actgttcatt tagaccagac aatgtattga tcaagtttgt
    88621 ctgactggtc aaattatctg tcactggctg ctcaacatca ttaacggaca acggtgcagt
    88681 aaatgctagt ggcaatgcat tgacattctg atacagtgta tagtcgccgc tttgaccagc
    88741 ttggcggaag ccatatttac tgaacgtgct ttcgttttta gaaatgttat atttgattcc
    88801 tgtaaaccca tccatcagca aagtattatt gttataacgg atattcaaat ttgtccctct
    88861 tgaacggaaa cctaatttat ccaggtaaga ggaagaatta cgatttctta tagaagaaaa
    88921 caaactgata ccactgtaac catagttgat cgaatcgttt gcagatactg gatccagatt
    88981 ttccaagcgg taaaacgagt cggaatcttt ttttgtttca tttacaagag ctttcaagtc
    89041 aggataagga tcagtatata aactgcggga agcataattc caatcatcaa gaatgccatg
    89101 gatcatactg ttcgtattga ccagtgcttc agcagacatg atcaataata acaatatact
    89161 cagatgattg aatgaaagtt ttttatgctg ataaaatccc acagcaaaag cataaatccc
    89221 taagaaaacc actgtcaata caaaggaagt catcgtcaca taggtgtagc ttccttcagg
    89281 tttgatgccc catgccaccg caaagagagc agctacgacc aaaatgattc ctaacaacag
    89341 cccacgatcg tcttgttcta tcttttccca tccttttcct gcaagcagga gcactaagaa
    89401 agagaacaga taggcataac ggaataaaaa catattcggt gcatgcatac catgccagaa
    89461 caaattcaat ggaacaagat aaaaacttgc gataagtatg gcgaaaagac tgccgtacaa
    89521 aaatttattt ttcaacggta ctttccgact ggcaaaataa aacagacaga ggactaatgg
    89581 tagcagacct gcgtaaacga acggaatcga tccgtatttt gtcgtgtcat aaacaccaat
    89641 catatttttc atgattagat ccagataaga agtcgcttct gttttcaacg tagtgatctg
    89701 actcagtgcc tcgccatttg accgaagatc cagcactgct ggcaatacaa tgatcattga
    89761 agcgccgcct gccagtaaag aagtgatacc ataaggaaca atcgtttttt tatattctga
    89821 ccagtttgtc aatagacggg cgataaaata taagaaagaa aatacgccaa tcataaaacc
    89881 catataaaaa ctggataaaa acaagagtaa ataactgata aataaaagtt tcggccgttt
    89941 aagatccata acacgatgaa tccccaaaat cactaatgga agataaacaa atgcatccag
    90001 ccacatgatg atctccgaat gtgcagtgat aaatgagatc aatgcgtaac tgacgctaag
    90061 tgcaacatgc ccccagcgag gaatcttgaa cgtacgtgca agaaaccaaa aagccaatcc
    90121 ggcacagccg accttaagca aagtcagaac atacagtgca tctggcatca tctggtttgg
    90181 aaaaaagaac accagtggcg tgaagatccc gcctagatag taagaaatta aagataaata
    90241 attcaatcct agagaagcat tccatgtata aaaaatactt tgctttccaa gaagaacatt
    90301 tcggaagctg gcatgaaaat tagaaaactg tgaaaaggca tcacttgcca aaatacttct
    90361 gctgcttcca ggatagattc cgatacttag ataaacaagt gctaaaatca aaaacggaat
    90421 caggaaacta gctgctgcgt agcgtccgtt ttctctaaag aatgacttta aatttttttt
    90481 catatttatt ttccaccaaa tttctatgaa gttgtattca gtttatcata tccttgtttt
    90541 tctctgctta aaaagagtct agttttcaca aaaaaataaa atttagaata aataacttca
    90601 taatttgttc gtataacaaa aataaacgca aaaaaagggt aggaatcaaa agtcccaccc
    90661 ttaaaaattt ttattaaata aaaaaccgtt cactatctga acggtccaag tatcattcaa
    90721 ttaaacgcgt tcaacttttc ctgatttcaa agcacgagtt gacacccaaa cttttttagg
    90781 ttttccgtcg accataacgc gaactttttg taagttaggt ttaacagtac gttttgttga
    90841 gttcatagcg tgtgaacggt tgtttccact ttttgtcttg cgacctgtaa agtaacaaac
    90901 ttttgccatg gtaatttcct cctttgcctt gatttggagc aatagaataa ttcagctcat
    90961 accagactaa catatcatat agaatgggaa tttgcaagta cattttcata gatacaattg
    91021 acaattgaga agaataattg taaattaaac aaggttatac ctgaaatttt gcaaggtttt
    91081 tgagtacttt gggtaactgc cttttatttc aagttgcgct atgataaaat atgatagaag
    91141 aaaaatggga gttttttacc cgttaaggag gctcattcat ggctgtaaaa attcaaacgt
    91201 cagctggaac cattgaaatt accaatgatg taattgcaac agttgttggt ggcgccgcga
    91261 cagatgtgtt cggtgttgta ggaatggcca gcaaaaatca aattaaagat aatattactg
    91321 agattttgcg taaagaaaat tactcaagag gagtagttgt ccgccaagaa gataatggta
    91381 ttgctgtaga tgtttatacg attatcagct atggtacgaa aatctctgaa gtttcccgca
    91441 acgtccaaga aaaagtgaaa tacaatctag aggcattatt aggcgtaact gccaattcag
    91501 ttaatgtctt tgtacaaggt gttcgtgtac tgccagacta ggcaggagac tggctggtaa
    91561 ctcagcagtc gatctgaagg aggattatct aggtgagtgt aacagaaata agtgcaagcc
    91621 agttccagga aatggtccag gcaggtgcga atcgtctgca agtaaatgca gaatacgtca
    91681 attcattaaa tgtattccct gtgccagatg gcgatacggg aacaaatatg aatctatcta
    91741 tgactagtgg agctaaggca gtagtagatt caacctcaga aaaagtagga gaattagcag
    91801 caatgctttc aaaaggattg ctgatgggtg cgcgcggaaa ttccggcgtt attttgtctc
    91861 aattgtttcg tggattttct aagcaaattc cagatgttgt ctcattaaat gcttctgatc
    91921 ttgctgcagc cttcactcat ggagttgaaa ctgcgtataa agcagtaatg aaaccagtag
    91981 aaggaacgat tttgacagta gctagagaag cagcgaaagc tggagaaaag aaagcaaaaa
    92041 gtactgatga tgtcgttgaa gtaatgacag ctgttgtaaa aggagcaaag cgtgctttag
    92101 ctaaaactcc agacttgctt ccagtcctaa aagaagtcgg tgtcgtagac agtggagggc
    92161 aaggactgct gtttgtttat gagggctttt taagtgcatt gaatggagaa ttccaagcgg
    92221 atgaaacata tgaaccttct ccagcagaaa tggatgatat ggtcaatgca gaacatcatc
    92281 gcagtgtcca aggtcaaatg gcgacagaag atatccattt tggttactgt acagaaatca
    92341 tggtaaaaat cggagaagga cctacagtcg atagtacatt cgattacgaa gaattccgta
    92401 actatttaga tggtatagga gattctttac ttgtggtcaa tgatgacgag atcatcaaag
    92461 tccacgtgca tacagaaaac ccaggagaag tcttgaatta tggacaaaaa ttcggttcat
    92521 tagtcaaaat caaagtcgac aacatgcgct tgcagcatga gacgattttg gaacacgatg
    92581 aagaagtgaa cgagtttgcg aaaaaagaaa taccacgtat cccgtatggt atcgtagcga
    92641 tcgctgctgg taaaggcgtc caagagctgt ttgaaagttt aggcgctaat tatgttatca
    92701 gtggtggaca aacaatgaat ccaagtacgg aagacattgt taaagcgatt caagaagtaa
    92761 acgcagataa agtgattgtc ttgccaaaca ataagaacat cttcatggca gctgatcaag
    92821 cagcagaagt agcagatatc ccagtagcag ttgttccttc aagaacgatt tcccaaggat
    92881 tgacagctat gctttcattc aatgatcaag cttctttaga agaaaacaaa gaaacgatga
    92941 cagaaatgtt gtctagcgtt gtgagcggtc aagtcactca cgcgatcaga gatacctcaa
    93001 ttgacggtgt agagatcaaa gaaggagact ttttgggcat gatcgatgga aaaatcctga
    93061 tctctaaccc agatatcaca gcaacagcgt taagtacact agaacaaatg atcaatgagg
    93121 acacagagat cgtaacgatc ttggcaggtg atgaaggaac agttgaacaa gcaaatgaat
    93181 ttgctgagaa actaacagaa aaacatcctg atttggaaat cgagatccac caaggagatc
    93241 agcctgtcta tccttatcta ttttctgctg aataatttct gtgtcagaat atctttctaa
    93301 cacagtattc attcataaaa ggccttcggt tagtgatcag gtatgatcgc tgaccaagtc
    93361 ctttttttgt taaaggaggg aggaaaaatt tatgcgttct ttagatgatc caatcactat
    93421 gctggcagga gtcggagaaa aaagagcagc tagtcttgcc agcttgggca tccaaacgat
    93481 tgaagactta ctgacttatt atccttttcg ctacgaagat attcaggaaa gaaatctaaa
    93541 cgagatccag gatcaagaaa aggttacttt gaaaggaatc gtagtctcgc ctcctgtgat
    93601 gaatcgattt ggttataaaa aaagccgatt gcaatttcgt atgatgcaag aacatgatgt
    93661 gttcaacgtt tcttttttta atcaacctta tctaaaagaa aaagtcattt tatcggagga
    93721 aatcgccgta tacggtaaat gggatgcaaa gcgtaaagcc ttgaacggga tgaagatttt
    93781 aggttctcaa acaatggatg acttttcgcc gatatatcac gtcaacaaat caattcgtca
    93841 aacgacatta gttgatctaa tcaaaaaagg gtttcaagaa tacggcacat tgattgaaga
    93901 aaatctgcca aacaaccttg tggagaaata tcgactgctt gaccgagctt ctgctgtaag
    93961 aagtatgcat tttccaaaag accacgaaga aaaccatcaa gccaaacgac gtgtcgtttt
    94021 tgaagagttc tttttatttc aaatgcgtct tcaaggattg aaaaaagcag aaaaagcaga
    94081 aacgaatggt ttggagatcc tttatgatgt ccaaaaattg aaacaattta ctcaaaagct
    94141 accttttgaa ctgacaaatg cacaaaaacg tgtaacgaat gaaatctgtc gggatcttcg
    94201 cagtccgcag catatgcagc gacttttgca gggagatgta ggtagcggaa aaacagtggt
    94261 tgccgcgatt gcgttatacg ctaccgttac agcaggtttc caaggtgcgt tgatggtgcc
    94321 tacagaaatc ttggcacaac aacatatgga aagcttgcaa cagctgtttg atcccaatga
    94381 agtaaccatc gcattgatta cggggtcaac gaaagcaaaa gaacgacgtg agctccttga
    94441 aaaactagag caaggggaaa ttaacgtcgt gatcggcaca catgcattga tccaagatgg
    94501 agtcgaattt cagaatttag gtctagttat tacggatgag cagcaccgtt ttggcgtgaa
    94561 ccagcggaaa gtacttcgtg aaaaaggctg gcgaccagat gtcttattta tgacagcgac
    94621 tccgattcct agaacattag ctattacagc atatggtgag atggatgtat ctatcattga
    94681 tgaacttcca gctggacgta tcccgatcga aacacgctgg gtaagaacac ctcaattgga
    94741 cagtgtactg gattggacga aaaaagaact ggcaagagga catcaaatgt atgtcatctg
    94801 tccgctgatt gaagaatcag aagcattaga tgtaaaaaat gcagtggaaa tctatgaaaa
    94861 acttcgtgaa ctatttgcac caaaatatga agttggcttg ttacatggta aaatgaagaa
    94921 tcaggaaaaa gaagtaatca tggaagcctt caaagacaac cagatgcaga tattagtttc
    94981 gactactgta attgaagtag gggtaaacgt gccgaacgcg accgtcatgc tgatcatgga
    95041 tgcggatcga tttggattgg cacagctgca ccaacttcgt ggacgtgttg gacgcggaag
    95101 tgatgcttcc tactgtatct tagttgctaa tcctaaaaat gaactaggtg tggaacggat
    95161 gaaaatcatg acagagacca ccaatggttt tgtactaagc gaaaaagatc tggaattacg
    95221 tggtcctggt gaagtatttg gcttccgtca atctggtctg cctcaatttg cgattgctga
    95281 cttggtgacg gatggaaatg ttttggaggt agccagagaa gaggctgcag ccctttggca
    95341 aataaaggat tggcagatgc ttcctgagta tcagccttta gcgcaaagcc tccaaataga
    95401 aaatcaggat aatcgttatt tcgattagca gactgtgcta tactttatac tgatgacgga
    95461 aaaaggagtg gaaacaatga aaattgccgt agatgcaatg ggcggagatc acgcacctca
    95521 agcaattgta gaaggcgtgg ctttagcgca gcaagatttt ccagaagtag aattcttact
    95581 ttacggaaaa gaagcagaaa tcaagaaata cttaacaaat gaaaaaaaca tcacgattat
    95641 ccatacagac gaaaaaatca atagtgatga tgagccagta aaagcaattc gacgaaaaaa
    95701 gactgcatca atggtccttg ctgcacaagc agtgaaaaac ggtgaagcag atgctgtatt
    95761 ttcagcaggg aatacaggcg cgctactagc agcagggttg ttcatcgtag gacgtatcaa
    95821 aaatatcgaa cgccctggct tgatgtctac cttgccagta gtagggaaag aaggtgccgg
    95881 tttcgatatg ctggatcttg gcgctaacgc agaaaacaaa ccagaacatt tgttgcaata
    95941 cggtatttta ggttcttttt atgcacgtaa agtccgaggt atcggacgtc ctcgtgtcgc
    96001 actcttaaac aacggaacag aagcaacaaa aggtagtgaa gtgacgaaaa aagcctatga
    96061 actccttcag aatgaaacaa gcttgaattt tattggaaac gtagaagctc gtgagctact
    96121 caatggtgtg gctgatgtag tggtcacaga tggatttaca ggcaacgctg ttttgaaatc
    96181 tattgaagga acagcaatga atatgatgaa tctattaaaa tctgctattc taaatgaagg
    96241 gatcaaagga aaaatgggcg ctatgctttt aaaagacggc ttacgttctt tgaaagcaga
    96301 aatggattat tccaaacatg gcggtgccgt tttgtttggc ttgaaagcac cagtcattaa
    96361 aactcatgga gcaacaggcc ccgatgctgt tcgctatacg atccgccaaa tccacacaat
    96421 gcttgagaca gatgtagttg gacaattagt cgaacaattt gaaaaaagcg aagaatagta
    96481 tttggatatc tgaagcgatc cgttccattg aaaaaaaggt ggacattcct gaaaaatgcc
    96541 agtaaaatta gccatggtgt aggtaattga caagtgtatg attcttggag gtgaaaaagt
    96601 tgactcgcga agaagtattt aacaaagtag cgaaaattat ttcaaatcat tttgagatag
    96661 ataccgataa agtgacggat gaattgaaca tcaaagatga tttaaaagct gattcaatta
    96721 gtattatgga attcgtttta gagcttgaag atgaattcgg tacggaaatt tcagatgaag
    96781 acgctgaaca aattgaaaca gtcggtggcg cagtagatta tatcagcagc catcttaaat
    96841 aatttcaaat aaaagcaaag aaggataaa
//
LOCUS       DAFEOM010000004        93455 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.004, whole genome shotgun sequence.
ACCESSION   DAFEOM010000004
VERSION     DAFEOM010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 93455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 93455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..93455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.004"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            85..972
                     /locus_tag="LP524_000307"
     CDS             85..972
                     /locus_tag="LP524_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBL2233390.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="LP524_000308"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="LP524_000308"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBL2233391.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="LP524_000309"
     CDS             2274..2720
                     /locus_tag="LP524_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBL2233392.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="LP524_000310"
     CDS             2993..4345
                     /locus_tag="LP524_000310"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HBL2233393.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="LP524_000311"
     CDS             4516..4803
                     /locus_tag="LP524_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233394.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            5029..5472
                     /locus_tag="LP524_000312"
     CDS             5029..5472
                     /locus_tag="LP524_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL2233395.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5478..6662)
                     /locus_tag="LP524_000313"
     CDS             complement(5478..6662)
                     /locus_tag="LP524_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HBL2233396.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6930..7628
                     /locus_tag="LP524_000314"
     CDS             6930..7628
                     /locus_tag="LP524_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBL2233397.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7635..8312
                     /gene="nth"
                     /locus_tag="LP524_000315"
     CDS             7635..8312
                     /gene="nth"
                     /locus_tag="LP524_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBL2233398.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8438..8971)
                     /locus_tag="LP524_000316"
     CDS             complement(8438..8971)
                     /locus_tag="LP524_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233399.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9268..11649)
                     /locus_tag="LP524_000317"
     CDS             complement(9268..11649)
                     /locus_tag="LP524_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBL2233400.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11639..12286)
                     /gene="recU"
                     /locus_tag="LP524_000318"
     CDS             complement(11639..12286)
                     /gene="recU"
                     /locus_tag="LP524_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HBL2233401.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12350..12889
                     /locus_tag="LP524_000319"
     CDS             12350..12889
                     /locus_tag="LP524_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HBL2233402.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12979..13398
                     /gene="gpsB"
                     /locus_tag="LP524_000320"
     CDS             12979..13398
                     /gene="gpsB"
                     /locus_tag="LP524_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HBL2233403.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13420..13787
                     /gene="rnpB"
                     /locus_tag="LP524_000321"
     ncRNA           13420..13787
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="LP524_000321"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13996..15162
                     /locus_tag="LP524_000322"
     CDS             13996..15162
                     /locus_tag="LP524_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBL2233404.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15208..16704
                     /locus_tag="LP524_000323"
     CDS             15208..16704
                     /locus_tag="LP524_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HBL2233405.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16785..17468
                     /locus_tag="LP524_000324"
     CDS             16785..17468
                     /locus_tag="LP524_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL2233406.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            18113..19303
                     /locus_tag="LP524_000325"
     CDS             18113..19303
                     /locus_tag="LP524_000325"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBL2233407.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            19405..20358
                     /locus_tag="LP524_000326"
     CDS             19405..20358
                     /locus_tag="LP524_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBL2233408.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            20427..21776
                     /locus_tag="LP524_000327"
     CDS             20427..21776
                     /locus_tag="LP524_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL2233409.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(21959..22282)
                     /locus_tag="LP524_000328"
     CDS             complement(21959..22282)
                     /locus_tag="LP524_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233410.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            22411..23574
                     /locus_tag="LP524_000329"
     CDS             22411..23574
                     /locus_tag="LP524_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBL2233411.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            23644..24993
                     /locus_tag="LP524_000330"
     CDS             23644..24993
                     /locus_tag="LP524_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBL2233412.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(25038..25469)
                     /locus_tag="LP524_000331"
     CDS             complement(25038..25469)
                     /locus_tag="LP524_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2233413.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            25857..28499
                     /gene="alaS"
                     /locus_tag="LP524_000332"
     CDS             25857..28499
                     /gene="alaS"
                     /locus_tag="LP524_000332"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBL2233414.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            28699..29295
                     /locus_tag="LP524_000333"
     CDS             28699..29295
                     /locus_tag="LP524_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL2233415.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            29417..30565
                     /locus_tag="LP524_000334"
     CDS             29417..30565
                     /locus_tag="LP524_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBL2233416.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(30603..31016)
                     /locus_tag="LP524_000335"
     CDS             complement(30603..31016)
                     /locus_tag="LP524_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HBL2233417.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            31095..31526
                     /locus_tag="LP524_000336"
     CDS             31095..31526
                     /locus_tag="LP524_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HBL2233418.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(31625..31858)
                     /locus_tag="LP524_000337"
     CDS             complement(31625..31858)
                     /locus_tag="LP524_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HBL2233419.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHQ"
     gene            32041..33711
                     /locus_tag="LP524_000338"
     CDS             32041..33711
                     /locus_tag="LP524_000338"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBL2233420.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            33858..34244
                     /locus_tag="LP524_000339"
     CDS             33858..34244
                     /locus_tag="LP524_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233421.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            34231..34929
                     /locus_tag="LP524_000340"
     CDS             34231..34929
                     /locus_tag="LP524_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBL2233422.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            34934..35398
                     /locus_tag="LP524_000341"
     CDS             34934..35398
                     /locus_tag="LP524_000341"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBL2233423.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            35395..36306
                     /locus_tag="LP524_000342"
     CDS             35395..36306
                     /locus_tag="LP524_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBL2233424.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            36541..37080
                     /gene="pyrR"
                     /locus_tag="LP524_000343"
     CDS             36541..37080
                     /gene="pyrR"
                     /locus_tag="LP524_000343"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBL2233425.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            37094..38377
                     /locus_tag="LP524_000344"
     CDS             37094..38377
                     /locus_tag="LP524_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBL2233426.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            38390..39316
                     /locus_tag="LP524_000345"
     CDS             38390..39316
                     /locus_tag="LP524_000345"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HBL2233427.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            39335..40615
                     /locus_tag="LP524_000346"
     CDS             39335..40615
                     /locus_tag="LP524_000346"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBL2233428.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            40620..41696
                     /gene="carA"
                     /locus_tag="LP524_000347"
     CDS             40620..41696
                     /gene="carA"
                     /locus_tag="LP524_000347"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBL2233429.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            41701..44886
                     /gene="carB"
                     /locus_tag="LP524_000348"
     CDS             41701..44886
                     /gene="carB"
                     /locus_tag="LP524_000348"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBL2233430.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            44940..45716
                     /locus_tag="LP524_000349"
     CDS             44940..45716
                     /locus_tag="LP524_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBL2233431.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            45713..46645
                     /locus_tag="LP524_000350"
     CDS             45713..46645
                     /locus_tag="LP524_000350"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBL2233432.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            46642..47358
                     /gene="pyrF"
                     /locus_tag="LP524_000351"
     CDS             46642..47358
                     /gene="pyrF"
                     /locus_tag="LP524_000351"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBL2233433.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            47365..47994
                     /locus_tag="LP524_000352"
     CDS             47365..47994
                     /locus_tag="LP524_000352"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBL2233434.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            47982..48296
                     /locus_tag="LP524_000353"
     CDS             47982..48296
                     /locus_tag="LP524_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233435.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            48334..48975
                     /locus_tag="LP524_000354"
     CDS             48334..48975
                     /locus_tag="LP524_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL2233436.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            49018..49710
                     /locus_tag="LP524_000355"
     CDS             49018..49710
                     /locus_tag="LP524_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HBL2233437.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            49707..50609
                     /locus_tag="LP524_000356"
     CDS             49707..50609
                     /locus_tag="LP524_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL2233438.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(50767..52473)
                     /gene="efbA"
                     /locus_tag="LP524_000357"
     CDS             complement(50767..52473)
                     /gene="efbA"
                     /locus_tag="LP524_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HBL2233439.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDTIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            52663..53286
                     /locus_tag="LP524_000358"
     CDS             52663..53286
                     /locus_tag="LP524_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233440.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            53736..54785
                     /locus_tag="LP524_000359"
     CDS             53736..54785
                     /locus_tag="LP524_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL2233441.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            54782..55663
                     /locus_tag="LP524_000360"
     CDS             54782..55663
                     /locus_tag="LP524_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2233442.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            55666..56436
                     /locus_tag="LP524_000361"
     CDS             55666..56436
                     /locus_tag="LP524_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL2233443.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            56715..57569
                     /gene="aroE"
                     /locus_tag="LP524_000362"
     CDS             56715..57569
                     /gene="aroE"
                     /locus_tag="LP524_000362"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBL2233444.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            57747..58910
                     /locus_tag="LP524_000363"
     CDS             57747..58910
                     /locus_tag="LP524_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBL2233445.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            59027..59437
                     /locus_tag="LP524_000364"
     CDS             59027..59437
                     /locus_tag="LP524_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL2233446.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            59611..61839
                     /gene="topB"
                     /locus_tag="LP524_000365"
     CDS             59611..61839
                     /gene="topB"
                     /locus_tag="LP524_000365"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBL2233447.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            62010..62294
                     /locus_tag="LP524_000366"
     CDS             62010..62294
                     /locus_tag="LP524_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233448.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            62454..62690
                     /locus_tag="LP524_000367"
     CDS             62454..62690
                     /locus_tag="LP524_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL2233449.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            62687..63085
                     /locus_tag="LP524_000368"
     CDS             62687..63085
                     /locus_tag="LP524_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBL2233450.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(63752..64696)
                     /locus_tag="LP524_000369"
     CDS             complement(63752..64696)
                     /locus_tag="LP524_000369"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBL2233451.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(64992..65936)
                     /locus_tag="LP524_000370"
     CDS             complement(64992..65936)
                     /locus_tag="LP524_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2233452.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            66157..67371
                     /locus_tag="LP524_000371"
     CDS             66157..67371
                     /locus_tag="LP524_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL2233453.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            67393..69408
                     /locus_tag="LP524_000372"
     CDS             67393..69408
                     /locus_tag="LP524_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HBL2233454.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(69491..70864)
                     /locus_tag="LP524_000373"
     CDS             complement(69491..70864)
                     /locus_tag="LP524_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBL2233455.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(70890..71174)
                     /locus_tag="LP524_000374"
     CDS             complement(70890..71174)
                     /locus_tag="LP524_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2233456.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(71176..73191)
                     /locus_tag="LP524_000375"
     CDS             complement(71176..73191)
                     /locus_tag="LP524_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBL2233457.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(73370..73675)
                     /locus_tag="LP524_000376"
     CDS             complement(73370..73675)
                     /locus_tag="LP524_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HBL2233458.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(73764..74795)
                     /locus_tag="LP524_000377"
     CDS             complement(73764..74795)
                     /locus_tag="LP524_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL2233459.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            75002..75877
                     /locus_tag="LP524_000378"
     CDS             75002..75877
                     /locus_tag="LP524_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL2233460.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(76029..76871)
                     /locus_tag="LP524_000379"
     CDS             complement(76029..76871)
                     /locus_tag="LP524_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL2233461.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(76963..78840)
                     /locus_tag="LP524_000380"
     CDS             complement(76963..78840)
                     /locus_tag="LP524_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBL2233462.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(78837..80258)
                     /locus_tag="LP524_000381"
     CDS             complement(78837..80258)
                     /locus_tag="LP524_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBL2233463.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(80261..83650)
                     /locus_tag="LP524_000382"
     CDS             complement(80261..83650)
                     /locus_tag="LP524_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBL2233464.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            83907..85298
                     /locus_tag="LP524_000383"
     CDS             83907..85298
                     /locus_tag="LP524_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2233465.1"
                     /translation="MEKITRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(85371..86018)
                     /locus_tag="LP524_000384"
     CDS             complement(85371..86018)
                     /locus_tag="LP524_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBL2233466.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(86026..86712)
                     /locus_tag="LP524_000385"
     CDS             complement(86026..86712)
                     /locus_tag="LP524_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2233467.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(86718..87791)
                     /locus_tag="LP524_000386"
     CDS             complement(86718..87791)
                     /locus_tag="LP524_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2233468.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            88013..88687
                     /locus_tag="LP524_000387"
     CDS             88013..88687
                     /locus_tag="LP524_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2233469.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            88692..90032
                     /locus_tag="LP524_000388"
     CDS             88692..90032
                     /locus_tag="LP524_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL2233470.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(90181..91167)
                     /locus_tag="LP524_000389"
     CDS             complement(90181..91167)
                     /locus_tag="LP524_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HBL2233471.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            91891..92922
                     /locus_tag="LP524_000390"
     CDS             91891..92922
                     /locus_tag="LP524_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL2233472.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            93031..>93455
                     /locus_tag="LP524_000391"
     CDS             93031..>93455
                     /locus_tag="LP524_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBL2233473.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
BASE COUNT        29895 a        16574 c        18965 g        28021 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt tttcttggtt tcagattggg ttttcctaca aaatattttt atctttgttg
     4981 aataggaaac aaaaaaacaa tataatgaaa gaaaaagaag gtgtagaaat ggataaaaga
     5041 caactgctaa tagaagaata tctggattta gctaatgaga ttattcatcg taataaacca
     5101 gaaatggaaa aagaatttgc aggatttact ctaaatgaac tagaagtgat tgaacatatc
     5161 gggaaaatcc ctgatcctaa cgtgacaaaa ttagcgaatg ctagttacat gactagagga
     5221 gccatcagta agctaacaaa aaaactgata gctagaggga tcataaacac ttatcaaagc
     5281 gaagagaata aaaaagaaat ctattttcaa ttgacgccaa tcggacaaaa aataaggaat
     5341 agacacgagc aactccatca aaaatgggta cagcgagatg ctactgtatt tgaaaatatg
     5401 tcaaaagaag aatttgacac agtatttcgt tttatcggtc gatacagaga atttatacga
     5461 agtagcaaat gaagctacta ttttttattg tagtgataat aggcaaaaat cataagcaaa
     5521 aatccgaata ccgtcggaag aaatggtgct gcagcataaa gctgtcctgc gattagcggg
     5581 ccgatgacgc gcgatagaga ttgtatactt tgggtaccgc ccattacaag accttgctgg
     5641 ttatctgaga cagaattgga tagttggcca ttgaatacag gagtaaaaat agaatcaccg
     5701 aaaccataac agataatccc aaataaaaac aaaatcggca gtagaagaat tccagataat
     5761 gtgatgaata gataggcgag gatttcactg aacatcccta ttcttgtgat ctgctgttct
     5821 gaaaattttt tgagcaatcg aggcataatg aacaattgcg agacagtatc caagattcca
     5881 atcaaagaga agcttaaacc aaccaaccca gccttccact gaaaggcgtc aatcgaaaat
     5941 tgagaaaaga tagattgaag agagccagct gccaaccaaa cagcaaaacc tgctgtcaat
     6001 aaaggaataa cggatgaaat cttgaataat tgttgtaatt ggtgaaaagg tcttacatgg
     6061 gagaaactta aatctactga acgttttttt attggaaggc tttctagcat aaatgtgtag
     6121 ccataaacgg catttaagaa agtaaagagt gctccaataa atataggaac agaattcccc
     6181 aactcagcta atagaccccc tataatagga cccagcgttg ttcctatacc gactaaagca
     6241 cccatccatc caaaaacttt ggttctttca ttaggttccg tgatgtcggc aaagtaagca
     6301 tataaggtgg agatttctcc tgccgtcaat ccttcaatga tacgaccaag gaacaacatc
     6361 catatcgaat ttcctaaacc aaaaagcaaa tatccgatac ttgatcctaa taaactgata
     6421 ataaggatcg gatgacgccc aaaatgatca cttaagctac cgagaatggg ggcagataag
     6481 aaggcagcta gagcataaac agacatgagc aaagtgatat agaaagcttg ctgatgtatg
     6541 gagtgagaat aaggcagcgt taaaaacgga agtacaggac tgacgatagt aaatcctaat
     6601 ccagtgaata agacagagac gtatccaaaa actttagaat gtttatttgt gaaactgtac
     6661 atgtaaaaac tcctttatgc aataatgtta acttggaaac aaaaatagtg tatagtaatt
     6721 ttgtttcctt gtcaacattc taaggaacag cgaaatctac ttaatttatt tattccgaaa
     6781 agtgaagata cgcatcaaaa acatgaacaa acaaaaatca agtagacgac attgtaaatc
     6841 tgaccgatca gtgaagtaaa tagaaaagaa acctgttctt gcttcccatg ttttttttac
     6901 gtatactgtg attgagcgga ggagaaatca tgttgaattt agaaaattac ttaagcgctg
     6961 gacaaacagt catatctaat cttttactta aaaattatca taggatcggt ctacaatctg
     7021 aagaattttt atttgttcta cagttgcata tggcacagct tgaaggagat actttccctg
     7081 atttgcagat gattgctatg gatatgggaa tcaaacaaga tcagattttt caaatactgg
     7141 atcgtctcgt aacaaatgga tttatcaaga tagaaacgac gattgtcaac ggtaaaaaag
     7201 cagatcgata taatctttac cccatctatg atctattagg tgagtattta aaaacacaag
     7261 aaaaaaaata cgaagaaaaa acacaagaaa aagaaatcca gtcagtgtat caattatttg
     7321 aacaagaatt tggacgcccg ctttcttcga tcgagttcca gcggatcgga caatggctag
     7381 aagaagacca ttatcagccg gaaattttga agctagcact gcgggaagcc gtattaaatc
     7441 aggcttatag ttttaattac gttgaccgca ttttgctatc gtgggaaaga aaaaatctac
     7501 gtacaaaaca acaagtggaa gaagaccaaa aacgaagaaa acagcagctt cttcaaagag
     7561 aagtagaaca gtcagctcaa caagaagaat tgccgaaagt ctcactgaaa aattggttgg
     7621 aggagtagcg gaatatgtta agcaaacaaa aaacaatgga agctctagaa acgatgtatg
     7681 gaatgtttcc cgaagcacat ggagaactca agcacaataa tccatttgag ttattgattg
     7741 cagtgatctt aagtgcccag gcgacagatg tatcagtgaa taaagcaacg ccagatttgt
     7801 ttgcctcttt tccgacaccc gatgcgttag cagaagcttc aatcgatgaa atcattctta
     7861 aaataaaaac gattggtttg tatcgaaata aagcaaaaaa catcaaagcg tgtgcacagc
     7921 agctgattga acgtttcgat gggcaagtgc caacgtcaag agaagaattg atgtctcttc
     7981 ctggtgtagg cagaaagaca gcaaatgtcg tattggggga tgcattcggg ataccagcaa
     8041 tcgcagtaga tacccacgtg gaacgtgtct caaaacgcct gaggatctgc aaactagatg
     8101 ctacggtcat ggaagtagaa gaaacactga tgcggaaagt accacaagaa ttatgggtaa
     8161 agacacatca tacactgatt ttttttggta gatatcattg cacggcaaga aatcccaagt
     8221 gcgaggtatg tcctttgcta tcaatatgtc aagatgggaa aaatcggatg cgtctaaaag
     8281 aaaaggcgct aaaaaagaaa ccacgcctgt agacgtggtt tctttaattg caaataaatg
     8341 tatgaattat cttgttatta tggagaaaag ctgtttcgtt tatctttttt gcatgaaaaa
     8401 gtaaatgtac atactcagta gctaggatgg taacttatta caatttattg cgataaaaat
     8461 aaaagttgtt atttttattg ggaaaatacc cgatgtagaa aataatgcct tccaatatac
     8521 ctataaaata agtccagtat tccaaaggat tatataaata agtgacgtat ctatctaaag
     8581 gtgtaataaa aatgataagt gataataaaa aaataccatt tataactaaa cataaactaa
     8641 tggatttggg cggtttcagg ctcatcagaa aaatatagat tctggtgaac aacaaccata
     8701 aaaaagtgag aatcattgca tgagagcttc tttccataaa ttttccttta agagtagtgc
     8761 ttaaaagtac agaaaaaata ataaatgcaa ggataggagc aattaaagta aatttattta
     8821 ataataaaca aaatttttca gcttttttat tcttggaata acgaggtatt gttttcgata
     8881 aaaaaagatg gtgcagacaa attatgataa ttaagcatac tatcagataa ctcaaaatga
     8941 agccttcttt caaaaaatta ttcaaaacca tatattgtat tttttatttg ataataacaa
     9001 tgggattatt aagaagtggc actaaataaa agactagtgt gtatttagcc gatttaatta
     9061 taaacaagta aatggataaa gtatatgtaa tgataaaaga aaaatggatt ttctaatttt
     9121 taagctaaat gttagatcac acatgtatat gctggatagt cttataaaaa atcatgtaat
     9181 acagcttggt acatgatttt tatttgtagc tgttttttac atagacacaa aaaagaaacc
     9241 acgcttgtag acgtggtttc ttttttacta tctgtttcca gaagctgagg tgctgctgga
     9301 tggtgtatgg tcatttgcag catccgctcc gctagttgct ggctcactgg aagaagcagg
     9361 aggagcagat gaagacggtg tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga
     9421 agacgctggc tcgcttgttt ctgctgtcgt gctttctttt gaggactcag cagttgattc
     9481 gctttgacta gtagagctag agctttgagt cgttgtctct gttgttgagc ttccaggagt
     9541 ctggacatag ctggaagatg gaatagttgt tgatggtgta attgcattcg atctagctgt
     9601 atattgatct ttgtagtaca gttcaccgcc cacacgaatt aatccgcttg gcatctccca
     9661 gtctgtattt gtcacgttag ctgaaacata ctgcataagc tcgcgataaa catcagaggc
     9721 tacgtgtgac gattctgaag tgacaggtgt cattttttta ttgtaacctg tccacacgga
     9781 aatcgaataa tttggcgtat agcccgcaaa taggatatct ggatacaccc cagaagaaat
     9841 accaagtttt gcatactcgt catctgtgta attggatgtt ccggtcttac cggcttgata
     9901 taacccagcg atttgcgcat tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc
     9961 tgtgatcata taagctgttt ctggagacat ggctgttttt ccatctggct cataagtttc
    10021 ttctgttccg tcttggaaga cgattttatt aacatattga ggtttgtaat aagtccctcc
    10081 attggcgaaa gccgcatagg ctgctgccat ttttaaggaa gaagctccgt attttgtccc
    10141 atcttgttct tccgtattac tagagatcgc attcgattga tggatcgtac tgtattcgat
    10201 cccaagattt tttaagaaag aagccacttt atctgaaccg acttcattga ataatttgac
    10261 tgctggaaca ttacgggata agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg
    10321 attatcccag tttcctacag gagtcgaagt cccttcatag ttatacggtg cgtcagtaat
    10381 agtttttcca gtagaatatt tcaaatattc aaaagctggt ccgtaatctg tgacaggttt
    10441 catcgtagaa ccaaaatcac gggaagtatt cactgctaag ttgttgccta aagttacgtc
    10501 ttctgcgatg tgacgtccgc caatctgggc ttttactttc ccggtattgg tgtcgataag
    10561 ggtggaggca acttgcatct cgtcgtctgg gtaagagaca tactgatccg tgttgacgat
    10621 atcatatagt ttcttttggg cgtccagatc caaattcgta taaatctcta aaccgtctgt
    10681 gtagacattc ttatctgttt tttcttgtac ttcattgatg acttctttga cataattatc
    10741 gacgatctta gtattgtcat cggattgagt tagttcttgc agaccatccg ttaccggtac
    10801 attaacagct tgatcatatt ctgtttgaga gattttttcg ttttgaagca tcgtatacaa
    10861 aacagtatct cgtcttttct tcgcctgatc tggataaaca taaggatcat aggcggaagg
    10921 ggcttgaggc atcccagcaa gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt
    10981 accaaagtac atctcagaag ctgtctccat cccgtacaag ccgtttgaca tatacacttt
    11041 atttacgtag taggtcaaga tctcttgttt cgattttttt tgttccaatc ttacagccat
    11101 ccacgcttct tgggccttgc gtttcagagt ctgatcctcg gcactggtgg agaagaagga
    11161 aagtttgatc aattgctgtg tcaaagtact tccgccttgc agtccgccag aggtaaagtt
    11221 agagagggca gaaccgatga tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg
    11281 atcttctacc gacacgatcg catcttctaa tgttttcggt aattcattcg cagaaatttt
    11341 ttcacgcttt tctgcgccta aatcttcgaa tagttcgcca tcttgtgtat atagtttgga
    11401 agatacggta gcgtctaatt ttttatccgt caattcgggc gcatcttttg cgtaatacca
    11461 gaaaagtcct acacctgcta aaaagaggat acagaaaaat gagagtattc ctaaaaggat
    11521 cttgataaat aatccttttt tatgtgttcc agaggacttt ccagaacctt tgccagaatt
    11581 cttcttcaca ctctttttag gagtaggctg tttctgtcgt ctagagcttc tagtttgttc
    11641 atttgccatt cttgctttct ccttcaatag ataatgttga taaatagaag tcaaccgcgt
    11701 ccaaataagg aatccgcgga gaaataccag ttttgatctc tattcctagt tcttcgatca
    11761 aagataaagg tagtgatttg cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat
    11821 gaacactatt caaataaaaa cagcgcttca aagacgaaaa ccataacaag acaaagcaga
    11881 ccccattttg ttcgaggcat gctttcatat gttcgatttg atgggcatgg aagtttttaa
    11941 agggaaaaga tgttttgttc ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat
    12001 agacgccatt atagtctgta gttgaggctt gggtaaagta ggcttccttg atcacagcag
    12061 cacttctctt aggatagtct actttcacga tttggatagg ggtaggcttt ttatggataa
    12121 ccgccaaccc gcgggaaaga taatatttat tactttcatt gattgcttct tcaaagcgca
    12181 tcccccgatt gccgaattca gtgatctgat ttcttttttc ttgaattttt tcgacgggag
    12241 aggcattctt ataataaggt tggccatttg gatagcgcaa taccatcttt aatcacactc
    12301 ctaggccaca ttatagcaaa aaagcggata gaaagaaaag gaatctgaga tggataagtc
    12361 aaaagtaatc tatgtgtctg ggtatcgaag tttcgaactc ggtgttttta aagaaaatga
    12421 tcctaaaatc caagttgtta aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga
    12481 agggctggaa tgggtacttg tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat
    12541 agtagcggaa ttgaaagaag aatatccgga actatctctt ggtattttgt atcctttttc
    12601 tgattttggt aataactgga atgaagggaa ccaagaaaaa aaacaattag cagaacaatt
    12661 agcagattat gtcgaagctg ttagccatca accctaccaa tcacctagtc agctaaagaa
    12721 tcacactcgt tttatacttg agcatacagt gggctgtctg ctgatctatg atgaagaatt
    12781 ccctggaaaa acgaagtatt tttttgaaga tgcacagaat taccaagcag atcatcccta
    12841 cgaaattcgg ctgatcagta tggacgatct tcaaaatttt agtgaataat cgatattgtg
    12901 tttgatttat caactttttt cctatacaat gaaaaaggta gaaatgttat tacgtaaatg
    12961 aaaactagag gtgtaaatat ggcgaatttg gtttatagtc ctaaagacat tttacaacaa
    13021 gaattcaaaa caaaaatgcg cggctatgat cccgttgaag ttgatgaatt cttagacaat
    13081 atcatcaaag attatgaaac ttacagcaaa gaactccttg ctttacaaga agaaaacgat
    13141 cgattgagtg caaaagtagc tcaattatct aaaactcaag gagcagctca aacacgcgtg
    13201 caacaaacag aggcaccaaa gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg
    13261 tcaaatcttg aacgtgaagt atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa
    13321 ccagcacaac ctaatccaaa taactacaca aatgcagaca caagcttaga tgataatgaa
    13381 aaaacacgtc aattttaatt tttgattaat gaatttgcgg ttttcgggta atcgcggtct
    13441 gacttgatca ggctgaggaa agtccatgct cgcacaggct gtgatgcctg tagtgttcgt
    13501 gcttagcgaa accataagct aaggtactct ttggagtaac ggcaggaaaa caagctaagg
    13561 cttcggctat gcttaagtat ccttgaaagt gccacagtga cgaagcggct ggggaaaccc
    13621 atccggtgga acgcggtaaa cccctcgagc gagcaaccca aacaatggta ggggcgcttt
    13681 ttgtctggaa gaagaacagg acaaaaaggc agtataactg cagatagatg gttaccgctt
    13741 tgttttttct ccctggaaaa gcaaagtaca gaacatggct tatagaaaac cgcaaacatc
    13801 agggaattag gtcgtgacaa aagtaatgcg ttataagaaa caagaaaaac atttgaaagc
    13861 tatttttcaa tacttcagat gaatcgttct tattacaaaa caaacttttg tatcaccggt
    13921 tattcggaat ttgagtaaaa gtgtggggta ggactgatgt cccacctcct ttttttataa
    13981 ataaggagat tgtgtatgga aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc
    14041 gggctagaag cactggttgg taaagaatta agagatcttg gaatcgaatg ccaagttgag
    14101 aacggacgtg cacgttttga aggaacaatg gaaacgatcg ctacagctaa tttatggtta
    14161 cgtacagcag atcgagtaaa aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa
    14221 ttgtttgaaa acgtaaaagc attgccttgg gaagattttc ttccactaga tgcagctttt
    14281 cctgtagctg gaaaatcgat caagtcgaaa ctatatagca cacctgattg ccaagcgatc
    14341 acaaaaaaag caatcgttga acgtctccgt acctattatc accgtccagc ttctgtacca
    14401 ctgacggaaa ctggcgcaca ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg
    14461 gtaactctgg atacgacagg accaagttta ttcaaacgtg gttatcgctt agaaaaaggt
    14521 ggagcaccat tgaaagaaaa catggcagca gcacttatta tgttgacaaa ttggcgaaaa
    14581 gaccgtccat tttatgatcc tgtatgtggt tcaggtacgc tttgtattga agcagcctta
    14641 ataggccata atatcgcacc aggattcaat cgtgagtttg cttgcgaatc ttgggattgg
    14701 ttcagtcaag aaatcatgga aaatgttcga tcagcagccg aagaaaaagc agattatgat
    14761 attgaattag atattactgg ttcagatatc aatggacgga tgattgaaat tgccaaagcc
    14821 aatgcagaag aaatcggatt aggctcttcg attaccttta agcaacaagc agtcaaagac
    14881 ttcaaaacgg ataaagaata cggggtcatc gtggctaatc cgccttacgg agaacgtcta
    14941 ggagaagaag aaactgttcg gaaattatat aaagaaatgg gcgatgtatt ccgtccatta
    15001 aaaacatgga gtaaatatat cttgactagc gacttagcgt ttgaggaatt ttacggacaa
    15061 aaagcaacca aaaaaagaaa attgtacaat ggtgcattgc gtacggatct atttcagtat
    15121 tggggaactc gtccgcctag aaaaaaagag aaagacgaat aatcttacta attgattcat
    15181 tcaattggat gtttggagga atagggaatg aaagaaaaag agtttttaca agaagtaaaa
    15241 gaaatcaatc ttctacaaca agctttagga atcctcgatt gggatacaca aacaggtatg
    15301 cctgaaaaag ctagtgcata ccgaagtgaa gtagacagct atctttacag catttatttt
    15361 tcaaaaaaaa tcggtccaaa aatcaaagaa gccattcgct atttttctga acatccggaa
    15421 gaattgtcgg aagttggaac tgccgttttt gccaaagtaa aagaagagta tgatttggaa
    15481 catcgcgtac cggaagatct tatgagaaaa tatacagctg ccgtttcttc agcacacgga
    15541 caatggcaaa aagctagaga aacacaacaa ttttctgatt ttcaacacgc attgaatcaa
    15601 aatattgaat taacaaaaca gttgatacct tactggagaa aagatgaaaa gacgaattat
    15661 gatgttttgc tgaatcaata cgaacccggg atgactgtcg aaatccttga taaggtattt
    15721 gatcagctta aagaaggaat tctttcgatc cgccgtacgc tccaagaaaa agggactgaa
    15781 cctgaaacag actttttaaa tcggagaatg acgaaaaaac aacagaagag atttgtagtg
    15841 aaagtaatcg aacaacttgg ttatgatttt agtcgtggtc gtttagatga cactgtccac
    15901 ccatttatga ctggaattaa tccgaatgat gcacgaatca cgacacgatg gaacgaaaca
    15961 gatttcaaaa tggctgtttt tggagtgatc cacgaggctg gtcatggtat gtatgaacaa
    16021 gacatcgatg ataaatatgc gtatacaccg gtttatgaag gtacatcaat gggtatccat
    16081 gaatcacaat cgttgtttaa cgagatcatt attggaagta gtcgcagctt ctggaaaaag
    16141 caatatcctt tctttcaaga atgtgcagaa ggaacattcg atgatattcc ttttgacaaa
    16201 ttttatcgtt cattgaacca tacaaaatca agtttggttc gtatcgaggc agacagtttg
    16261 acttaccctc tccatatcat cattcgttat gaaatcgaaa aaatgatttt caatgaaggg
    16321 gtagatgttg ctgaactacc tgaaatatgg aatcaaaaat atgaagagta tctgggaata
    16381 cgaccaacaa atgatgtgga aggcatccta caagacgttc attggtcagg tgcaagcttt
    16441 ggttattttc cctcttatgc attagggtac atgtatgcgg cacaattact tcattctttg
    16501 cagcaagaaa tagatgtgga aaatgttcta gcgtcagacg attattctcc aatcaaaaac
    16561 tggatgtcag agcatatcca tcaatatggt gcatctagaa aaccaaatca attaatcatg
    16621 gatgcaacac atgaaccgct taatccgcaa tacctgatcg actatatgaa agacctgtat
    16681 tttaatgtat atcaggtaaa ataaaataca atttacaaag atagctgaaa ttcatctaat
    16741 ctatagatgt tttcagctat ttacttaaga aagaagtgga acgaatggat aaattacaag
    16801 tgatcgctga ttatagtatg gaccaattag cgcatgatca aacaggacat gggagcgatc
    16861 ataccaaaag agttgtcaag ttagctgaaa aaattttgga gacggaaccg caggcagacc
    16921 gctttgtgac gcttgcagca gcttacctac atgatacgat tgatgataaa gtcgttaaaa
    16981 atgagaacga ggcaaagcaa cagcttcgtg tattccttcg ctctttgcca atagtagagg
    17041 aacagatctc gatgattttt tccattatcg aaaatatgtc cttttctaaa aatctctctg
    17101 aagcggccga actatctctg gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat
    17161 taggagcaat cggcattttg cgtactgctt atttcggcgg tggacatggg cacccgattt
    17221 ttgatcctga actgtatccg caaacattca aggacaaaga aaattaccga aaaggcacga
    17281 cagtgatcaa tcatttttat gaaaaactgt tcttgctgtc tgataaaatg aatactgatt
    17341 acggatatga agaagccaaa cgaagagaaa ccttcatgcg tgaattctta gaagaatttt
    17401 ttctggaatg gtctattgac gatgaaaatt ttactccaga caattggatc aaaaataaca
    17461 aaaaatgatg gaggttgtga cagaagtggt cagctccaag atatttcgga tcattttccg
    17521 taaaagttac ttctggaaca ctgtttacca agaggtcgtg agcctgccgt tcagctccga
    17581 gtcacaagga acaatttcaa aaaatagctt ctcgtgtttg atgaatattg taacttgtga
    17641 ttcggaattg gcaggagaac accgtttatt cggaagaata ggactgtgac aagacttttg
    17701 tcacagtcct tttttcgaat tcaacaagta aaacttttaa gaaatattca gagagtaaaa
    17761 ttaaaaattg ctgataaacg aagatggaaa ttttatgtat ttttattggt ctatgcaata
    17821 aataaattaa atattcagaa tacaattgat tttaacgaaa gggcttctat caatggttgt
    17881 gttgtattta tctgcccagg tagagtaaaa tagatcatta tctaaaaggt aattgttcat
    17941 actatataag tcatttgaaa gtaaaaagat actgtaaata aaaaatgtgg ttaaataatt
    18001 ctcataatca gtgtaagcgt tatcaaaaat atagcatact tgctttgtag atgaatgaca
    18061 cgatatatgt ttggtatgtt ctgcaagaca gatgaatatg gaggtggaga caatgtcttc
    18121 caaagaacta acaatgtatt tagagaaaga attggatcaa ctgaaagaaa aggggttata
    18181 caatacgatc gacatactag aaagtgaaaa tggtgcttgt atcatcgtag acggaaaaaa
    18241 gatgatcaat ctagcatcca acaattatct tggatttgcc aatcgcgaag aattgaaaaa
    18301 agcatgtatt gaagcaaccg aaacttatgg tgtaggagca ggagcagtcc gaacgatcaa
    18361 tggttcgctg aaaatccatc agcaattaga agaaaaaatc gcagaattca aagggacaga
    18421 agcagcaatc gcctttcaat ctggattcaa ctgcaatatg ggcgctatct ctgcagtaat
    18481 gacaaaagaa gatgctatct tatcggatga attaaaccat gcatctatta ttgacggttg
    18541 ccgcttatcc ggagcaaaaa tcatccgaat taaacatcaa gatatgaaag accttgagaa
    18601 aaaagcaaag gaagcgatag aaagtaagaa atataaaaaa atcatgtata tcacagatgg
    18661 tgttttctcc atggatggag acattgcacg attgccggaa attattccga ttgttgaaaa
    18721 atatggactg atcacctacg tcgatgatgc gcacggctca ggcgtaactg gaaaaggtgc
    18781 aggaaccgtc aagcattttg gattaagcga caaaatcgat atgcaaatgg gcacgttatc
    18841 gaaagctata ggtgttgttg ggggttatgt agctggatcg aaaacactaa tcgactggtt
    18901 gaaagctaga agtcggccat tcctattttc tacttcgcta actccaggtg cagctgcagc
    18961 tgcattggct tcgattactt tgatgcaaga acatccggaa cttgtcgaaa aagtttggga
    19021 aaatgctaat tattttaaag aagaattgaa aaaagtaggc tataacatcg gtatgtctga
    19081 aacacctatt actccagtca ttttgggaga cgaaaaagtg actcagactt tctctaagaa
    19141 gctgatcgaa catgggattt atgctaaacc aatcgtttat ccaactgtgc ctcttgggac
    19201 aggcaggatc agaaatatgc cgactgcgga acatacgagg gaaatgcttg atgaagcagt
    19261 agcagtttat caaaaaatag gaaaagaaat ggaaattatt taactaaatt tagttttttc
    19321 gtctatttaa aattagagaa catgttcacg catatattgt taatcatctg aagatagtag
    19381 taaaaaagat cagggaggat catcatgaaa aaagtattgg tgacaggatg tttaggacaa
    19441 attggttcag agttgatcac acggctacgt caagaactag gcagtgaaaa tgtcattgcg
    19501 acagatatac gcatgcctga agtcattgag aaaaacgata tctttgagat cctagatgta
    19561 atggattacg aaaaaatgcg cagcatagca gaacaatatc aagtggacac attgattcat
    19621 ttggctgctc ttttgtcagc tgttgcagaa gcaaaaccac aattagcctg gaatttgaac
    19681 gtgactgggc tggtcaatgc attggaagtc gctagagaat tccagctgaa attttttacg
    19741 ccaagttcaa tcggcgcgtt tggtccaaat actccaaaag atcatacccc acaagatact
    19801 cttcagcgtc ctacaaccat gtatggggta acgaaggtag ctggagaact actatgtgat
    19861 tattattatg tgaaatatgg cgtagatact cgtggtgtcc gtttccctgg attgatttct
    19921 tatgcaacct tgccaggagg cggtacgaca gactacgcag tcgatattta ttattcagca
    19981 atcaaagaag gaaagtacac tagtttcatt gatcaaggta catcaatgga tatgatgtat
    20041 atgccagatg cgatcgatgc cattataaaa ctcatgaatg cagatcctga tcgattggtt
    20101 catcgaaatg ccttcaatat atcggcgatg tcttttgacc cggaagagat caaagcatct
    20161 attcaaaaaa tcataccaga ttttgaaatg gattatgatg tggatcctgt acgtcagtca
    20221 atagcagaat cttggccaaa ttcactagat gtttcttgtg cgcaagagga atggggcttt
    20281 tcgccgaaat acgacttaga tgtgatgaca aaggatatgt tggaaaaact gaaagaaaaa
    20341 ttggtagaga cggtataaaa caagaaccac atccaaaaag ttttttatac ttttgggatt
    20401 ggctatggta tgaaagggga caagggatgg aagaatatca agccacgcca gtaaaagaag
    20461 taaaaataga aaatgaatta aaacgtacga tgggattttt tactgcttta tcgactgtta
    20521 tgggcacagt gatcggtgca ggggtatttt ttaaagccgc aagcgtagca gaagttacag
    20581 gatccgccag tttgcatatg ttttcttggt ttctcggtgg tatgatttct gtctgtgcag
    20641 gactaacagg agctgaactg gcagctgcta ttccagaaac aggagggatg attaaatata
    20701 tcgaacggat ttacggaaat acagcagctt ttttattggg ctgggcgcaa gtcgtgattt
    20761 attttccagc aaatgtagca gccttgtcga tcattttcgg tacacagttc gtcaatctat
    20821 ttggtctttc ccagtcaatg attgttcccg tagctgttac agctgctgta tcgatcctgt
    20881 tgatcaattt tttaggttct aaagctggag gggcttttca gtcgattacg cttgtctgca
    20941 agcttattcc tttgtttgtg atcgtcattt tcggtttgtt ccgacaagaa ggagtagact
    21001 tccaactgtt tccgatccaa gcaggagaaa atctttcatt tttctcagcc ttgggagcgg
    21061 gcttgctagc aacgatgttc gcatacgacg gttggataca cgtaggaaat atttcgggag
    21121 aattgaaaaa gccggcgaaa gatttgccaa aagcaatttc tttaggaatc atcggaatca
    21181 tgatcgttta tttattagta aatgcggtgt tcttaagaac cgcatctatc gacggagtcg
    21241 caggaaacag caatgcggca agtgacgttg caaaaatgat ttttggcggc ttcggtggaa
    21301 gactggtcac agttggtatc ttgatttctg tttatggaac catcaatggc tataccctta
    21361 ctggaatgcg tctcccttat gttatggcaa aagaaaacaa cctgcctttt agcaagctct
    21421 ttgctaaact tcatgataaa actaaagttc ctgtagccgc aggtatctta gagctagtca
    21481 tcgctattgg catgatgatg atcggtggtt ttgatacact aacggatatg ctgatctttg
    21541 tcatttggat tttttatacg atggtctttg ttggtgtcat tcttcttcgt aaaaaagaac
    21601 ctgatttgtt tcgaccatac aaagttccaa tgtatccgtt tattccacta gtagcaatca
    21661 ttggagggac ttttatcgta tcaagcacgc ttatcactca gaccttttta gcttcgatgg
    21721 ggatagtatt gacacttgct ggtatcccga tttatctcta tttaaaacgt agatagctaa
    21781 aacattcgaa ttttattaga actgcacttc tctgccaaac aaaatatttt atttaaatga
    21841 ggagtacgtg gaagaaatgt caaaagtact cgttaatttt gatcacgtaa atgtcgtttt
    21901 gtctacgatg aaatagcaaa gtaaaagaca gaaatcgggc tcaaccgatt tctgtctttt
    21961 actttgattt gcgtaactga actattcggc gtacgccgcg ataaatattt aataaaacca
    22021 agcaaccaga aatgaaaatg ggaagccaaa tcagatagac agggtagcca agcactttag
    22081 acaaagcatg tggagtcatt ttatccattt caagaagtgc ttgctgccaa gtctgaagat
    22141 tgaagactag catgaaaccc gaaataatca ggagaataaa tcctaaataa tgaattgtag
    22201 ttctttttct tacataagcg atcgtagcat aaaggaacag taaaaaaata ggtacgccaa
    22261 aaacaaagta catattcagc atctcgacac cccctttgtg tttatcttac tttactttga
    22321 aagaaatgag aaataatagc ctcgttactt gcttgaaaca gcaaaggact gctaagataa
    22381 gagagataca aagaatcaag gaggagtcat atggcaagtt atatcgaacc gattcgtttt
    22441 gcgctgattg tttttccatt tttagcctta gcgatctcat ctgttttttt tatttatgaa
    22501 tatcgaaagt atggaacatt tctagtaaca agggcagtta ttttgtattc tttcgttttt
    22561 tatttattgt gtgcgtattt tttggtgatc ttaccacttc cggcaaagga gacggttgcc
    22621 caaatgacag ggccaaagat ggagctgcat ttatttgctt catggcaaca tttcagagac
    22681 aacacggtat tggtcttgtc tgaacctagt acctacctgc cggcaatgaa gcagagtgta
    22741 tttttggaac cagcttttaa tgttttatta ctcataccat ttggcattta tttaagatat
    22801 tatttccggc tttcttttat gaaaacgatg attgctagtt tctgcctttc tctatttttt
    22861 gaattgacgc agctaagtgg actttatttt atctacccaa gaccataccg cttatttgat
    22921 gtgaatgatt tagtgacaaa tacattgggt ggaatcattg gttttgtcgt tacgccgtta
    22981 tttacattta tgcttcctac aagaagacga atggatgaac tttcttatga aaaaggaaag
    23041 actgtctcat tgactcgacg cttggtcgct tatttgatcg actggacatt tttatctctt
    23101 attaatttgg tagcggcgat tttctttaaa attgcgttcg attcaggggc agttacttct
    23161 catatctggt ggcttttatt agaagtaggg gtatacttta tgattgtacc gtttataacc
    23221 aatggatgga cacttggcaa aaaaattgtg cgtatccgtt tggtggaaga agaccgcgaa
    23281 cgaatttctt taaaagcatt gatgatccgc tacgggtatc tgtactttct tttttatggt
    23341 ttaagctggt tggggcagtt gcttcagcaa atcatgaatt cgacaacgaa tacggtgttg
    23401 cagatgagtg cagtattcat ttctctcggt gtcttgttac tacaaggtat ttttatttta
    23461 aatatcttgc tatcattttt gagaaaaaaa cggattctgt tttatgaaaa agctagtcac
    23521 acatatactg ttagcacgat tggccaaaca aaggatgatg attctcgaaa ttagctgagc
    23581 aaaaacgctt tgtctgtgtt ataatgccaa agtcgtgata atttaaagag aagaggtgca
    23641 cgaatgaata attttgatat attccgcttc aaaccgttta tcaatgaagc gttaaccgca
    23701 aagggcttca aacagcccac agaggtccag gaacgtttga ttcctttaat tgcaaaagga
    23761 aaaaatatca ttgggcaatc tcaaactggt acaggaaaaa cacatacgtt tttgttgcct
    23821 ttgtttgaaa aaatcaatgc agaaaaagag gaagtacaag ttgttattac agctccaagt
    23881 agggaactag ctgcacagat ctatgaagca gctacacaaa tcgctaaatt ttctacaccg
    23941 gagatccgtg tctcgaattt tgtcggcggt acagacaaac agcgtcaatt aaatcgtctg
    24001 catcaccaac agccacaggt tgtagtaggc acgcctggta gaatattaga catgatgaat
    24061 gaacaagcgc tgaagattca caccgcattt gcatttgttg tcgatgaagc ggatatgaca
    24121 ttggatatgg gttttttaaa tgaagtagac caaattgcaa gtcgattacc agaaaagctg
    24181 cagtttttag ttttttctgc cactattcca gaaaaacttc gtccattctt acgaaaatac
    24241 atggaaaatc caatcattga agaaatcaaa ccaaaagcag ttatttctga agacattgaa
    24301 aattggctga tttcaacaaa aggtaaagat gagaatcaaa tgatttatca actgctgaca
    24361 attggtcatc cttatttagc aatcgtattt gccaatacaa aacagcatgt ggatgaaatt
    24421 gccgattact tgaaaaacca aggcttgaaa gtagcaaaga ttcatggaga catcacccca
    24481 agagagagaa aacgagtgat gcgacaagta cagaatctgg agtatcaata tgttgtagcg
    24541 actgatttag ctgctagagg gatcgatatc gaaggtgtct ctcatgttat taatgcagaa
    24601 attccagaag atctagactt ctttatccat cgtgtcggac gtactggacg taatggctta
    24661 aaaggaacgg ccattacgtt atattcgcca aatgatgagc aagccattga tgcgatagaa
    24721 aaattgggcg tctcatttga accaaaagaa atcaaaaacg gggaaatcgt caaaacatac
    24781 gatcgtaata gacgtgccaa acgagaaaaa tcaaaagatg tattagatcc cacgctgatt
    24841 ggtcttgtga agaagaaaaa gaaaaagatc aagcctggtt ataagaaaaa aatcgattgg
    24901 gcaattgcag aaaaaaataa aaaagaacgc aaaatcgaac gcagacagca aagccgtact
    24961 gctcgtaaaa ataagaaaaa ttcacataaa taaaaaacaa gaggttgtga caaaaggcgt
    25021 gtcacaatct cttgttttta agctagactt tttttcattc cgatacaaag tacttcatct
    25081 tcaataaaag gttctgtgca agtatgatac ccttgttttt cataaaaatc tacagccgtc
    25141 atttcagcta ctacataact agtctgacaa ccttctgaaa ccgcccgttt ttcagcagct
    25201 tcaagcaact gaccgcctaa cccttttcct cggtattctt ttgctacgca aaatcgttcc
    25261 ggcttgaagc aagtaggtgt tttcttttca taacgcaaag tagctactgg tcttttttga
    25321 tcaagaaata gataataggt ttgttgcggg atatcttttc cgtcgaattc tagttctgca
    25381 ggaatccctt gttctaatac aaaaacttga tatctcaaaa agcagctagc tgcgcgtatc
    25441 cattcatccg ctccaactat ttttttcatc atattcctcc ttgtcatgaa tagcagagat
    25501 gtattgattc ctttattctt atcgtaccaa aaatcgtgga gtatgaccaa aaggaaatga
    25561 aaataaaaat tgagatattg acagtgatca atagaatgcg tatactaaat aagtacgaaa
    25621 aggatatgat ttgtttttta agactcacag aaaattcttt cttggctgaa aaaagaatag
    25681 tcactaatca aattgctccc tttttctatt ttggtagaaa taccgacgca gctcagcgtt
    25741 atcgagttta agaggtaatg attacacatc attgcaatca aggtggtacc gcgagttttc
    25801 gtccttggct gtgatggtgt ttttttgtat ttaatttata gaaagcaggt aatgacatga
    25861 aacaattaag tagtgcacaa gttcgacaaa tgtttcttga tttcttccaa tctaaaggac
    25921 atacgattga acctagtgcg tctttgatcc ccgtgaacga tccaacctta ttgtggatca
    25981 actctggtgt tgcaacattg aaaaaatatt ttgatggttc agttgtgcca gaaaatccaa
    26041 gaatcacaaa tgcccaaaaa tctattcgaa caaatgatat cgaaaatgtc ggtaaaacag
    26101 cacggcacca tacgatgttt gagatgctgg gtaatttttc gattggtgac tatttcaaaa
    26161 atgaagccat ccactgggca tgggaatttt tgactgatcc aaaatggatg gcattcgatc
    26221 cagaaaaact ttatgtaaca gtttatccaa aagatacaga agcaaaacgc atctggcatg
    26281 aagaagttgg cctgccacta gatcacatta ttgatgtaga agacaacttc tgggatatcg
    26341 gtgcaggccc atgtggtccg gatacggaaa ttttctatga ccgtggagaa gcttataacg
    26401 atttagcaga agacgatcct gaaaactatc caggaggaga aaatgaacgt tatttagaaa
    26461 tctggaactt agtattttca gaattcaacc ataagcctga taacacattt gaaccacttc
    26521 ctcataaaaa cattgatacg gggatgggat tggaacgcat ggtctcaatc gttcaagatg
    26581 cgtcaacgaa ctttgagaca gacttgttta tgcctattat ccacgccgta gaaaagctaa
    26641 gcaaacaagt aaaatatggt gaaaacccag taacagatat ttcttacaaa gtaatcgcag
    26701 atcatattcg cgccctttca tttgctattg gcgacggtgc attgccatcg aatgaaggga
    26761 gagggtatgt cttacgccgt ttgcttcgtc gtgctgtcat gcatgggaaa aaattaggta
    26821 ttgaacaagc attcttatat cgtttagtcc ctgttgttgg ggaaatcatg gtaagttatt
    26881 atcctgaagt acttcaacaa aaagaattta ttgaaaaagt tatccgtaca gaagaagaac
    26941 gtttcaatga aacaatcaat gaaggtttgg aaatcttaaa tcaagtgatc caagaagtaa
    27001 aaaacgaaaa cgggacgact ctagcaggaa aagacatctt caaactatac gacacatatg
    27061 gcttcccggt agaattaacg gaagaggtgg cagaagatga aggactgaaa gttgatcatg
    27121 ctggttttga aaaagaaatg gatgcacaac gcgaacgtgc tcgctctgcc cgcagtactg
    27181 aaaaatcaat gggggtacaa tctgctttat tgacagatat caaagtagaa agtaaattca
    27241 ttggatacac agaacaagaa tcagatagtc aattattggt catcgttaaa ggtgagtctc
    27301 ttttgaatga agtcgcagaa ggtgaagctc aaatgatctt tgatcaaaca cctttctatg
    27361 cagaaatggg tgggcaagta gcggatcaag gatggatcat gaatgaagta ggcgaaatcg
    27421 tagcagttgt tacagatgtt caaaaagcgc ctaatggtca atttatgcac accgtagaag
    27481 taaaagcacc aatgagtgaa ggacaaacct atcatctatt gatcgatcaa tcgatgcgca
    27541 atcggatcat caaaaaccat acagcaaccc atctactgca caaggcattg aaagaagtac
    27601 ttggcgagca tgcaaatcaa gctggttctt tagtcgctcc tggacattta cgattcgact
    27661 ttacccactt tggtcaaatc acttcagatg aattagctga aatggaaaga atcgtcaacg
    27721 aaaaaatctg ggaagctatc ccagttgaaa caattgaaac agatatcgat acagccaaaa
    27781 acatgggtgc aatggctttg tttggtgaaa aatacggaca tgacgttcga gtagtaaata
    27841 ttggcggctg gtcaatcgaa ctgtgcggag gcacacatgt agcaaataca gaaaacatcg
    27901 gaatcttcaa aatcgtttct gaatcaggaa ttggtgcagg cgtgcgacga atcgaggctg
    27961 taacaagtaa agaagcgtac gaactacttg aagaagaaga aaaacaacta aaacaagtag
    28021 caacaattgt taaatcacct caattgaaag aagttgtttc aaaagcggaa caattgcaac
    28081 agcaagtaag agagctacaa aaggaaaatg aacaattagc tagcaagtta gcgaatcaac
    28141 aagctggtga tgtattcaaa gatccagaaa cgatcaatgg aaaaacaatc attgcagcac
    28201 aagtaaaagt gaaagatatg aatcagctgc gtcaattagc tgaccaatgg aaacaaaaaa
    28261 atatttcaga tattcttgtt ttagcaaacg aacaagaagg aaaagtcagt ctgctagcgg
    28321 caatgacgaa agaatcaaat gaagctggac taaaagctgg cgacttgatt aaagcaatcg
    28381 caccgaaagt aggcggtggc ggtggtggac gtccagacat ggcacaagct ggcgggaaaa
    28441 atcctgctgg tattccagat gcacttaaag ctgcgaaaga atgggttgcg caacaatagg
    28501 gcgtttacgt ttgacagaaa aacaaaagtt caaacattga acgaaacatg attatggggg
    28561 cgaagacaaa caagtcttgc cctcatactt ttttcaattg gatggcgcat ctaatcaaat
    28621 ggtcggattc ggatggtctt tagttgacga aagtgacaga aatttttacg attagtataa
    28681 ataaaaaggg gtgaaaaaat gaatagtaga aataagacct ttcgactagt cttacgtgcg
    28741 atcttgttag caatcatcat cgtacaagcg atggttcctt ggctggggtt tattccatta
    28801 ggattcatta gtttaacaat tatccatatc acagtaatta ttgctgcagt agtattaggc
    28861 ccaaaagatg gcatggtgat tggcttgttt tggggaatcg ctacaattgt tcgtgcctat
    28921 gccatgccaa caacgccatt tgatacatta gtgttcacga atccgattat ctcggtagtt
    28981 cccagagtac ttgttggctt agtagcaggc ttggttttcc actggattta tcaaaaaaat
    29041 aaatcaataa cgatcagctc agcctttgca ggggtactgg gttcattggt caatacagtc
    29101 ttagtgttag gattcatggg attattttat acaggtgcaa cggcagatgc ctatggtgta
    29161 gactctgctc ttttatttaa aaccttagca ggggttgcag caatcaatgg gataccagaa
    29221 gcaataggtg cgggaatcat aacaccatta ctagcaaaag cgttatttgc agcaacaccg
    29281 ttgaaaccag aataatgacc atcttttaat agtaatagga cgcacgatag atagaaaaag
    29341 gaagagagga gaggaaaaca atcgtcctct ccatcttttt acctgagcaa caagcaaact
    29401 agaaggggta aaaaacttgg ctaagaaaca agaagaaaaa aagaaacaaa taccgcaaca
    29461 gcggacaaaa aaaggagtac tagcgggtat cttaggattg gttacgaatc tgatcttgtt
    29521 tgtctctaag ctaatgatcg ggcttttttc aggaagtgtc tccattatgg ctgatgcagt
    29581 gaacagtcta tcagatactg cttcatccat attgactttg atcggattta aaattgctgc
    29641 aaagccagca gatcaggaac atccttatgg acacgaacgc ttcgaatata tcagtgggtt
    29701 atttgtttcg atcatcatta cttatgtagg atttcaattt ttagagtctt ctgctgaaaa
    29761 aatcatccat ccagaaaatg tctctttaac acccatcgtc ttttttgtcc taatcatttc
    29821 catctttttg aaatttgcac aaggcaaaat gtatcaggtt attgcgcaat ccattgattc
    29881 tgaaacattg agagctacag caacagatag ttataatgat gtctttacta cactggctgt
    29941 attgctttca gcagcaatcg aatgggtaac tggatggcgt attgatggat atataggttt
    30001 tgctttagca ttatatatcc tttatagtgg attgacgatg cttcgaaatt ttgtatacga
    30061 actattagga agtcgcccaa cgccagcaga gattgaagaa atggaagagc atttaaatgc
    30121 ttgcgatgat atacttggct atcatgattt attggtgcat aactatggac ccaataaacg
    30181 ctttgcatca gtccacatcg aagtagatga atcaatgaat ttgaatgtgg ctcataagat
    30241 tattgatctg atagaaaaag actttaaaaa gtctttggat gtggaacttg tctgtcactt
    30301 ggatccaatg cctgttgata acgaaaaata ctataatatc ttgcggcagt taaagcaaat
    30361 tcttcttgaa acaggtacag ggcttaatat tcatgatttt agagtaattc atcaaggaaa
    30421 aacgctgcaa tttgatgtag tggtccctga gaaattccag tttgttgata cagaactaga
    30481 aagaatctta cgtagtcaaa tagaagaaaa aatcggagat taccaattgg aaattacgtt
    30541 tgatcacaat tatcttttat attaaatgag aaactataaa aagactcctt aaagttttta
    30601 atttaaagag tctttttttc ttttaattgt ttatggagag cctgacgtgc aagattatct
    30661 gctcctttat ttttgttttc gggcaaccat tgcaggatca ataaagaaaa ttcggataga
    30721 agttcttgga ttctttgaag gtagggaagg aagagctcgt tcttcgttgc gtttttgtcg
    30781 accgtagaaa ctagtgtttt gctgtctgaa aaacagaaaa tcgtttcatt ctgtaagtcg
    30841 tttttcttta gaaattctaa agtttttaaa aagacttcga actctgcttg atggtttgtc
    30901 cctatagaaa gcgggataga tagctgttct tgtttacctt gatagacaat caagattcca
    30961 cctccactag gaccagggtt gccttttgta gaggcatcaa tgtatacttt taacataaaa
    31021 agagattctc ctttttgttc aaactaatgt tatggtaaga ttatatcatt actgaatcgg
    31081 tgagaggtta gagaatgaaa aaaaatattc gatggcagcc tgaactggca catacgatca
    31141 tctattggtc attgaccttg atcattctgt tttttagtct gattctttct ttagaaaaca
    31201 caggtcctta ttggaaaagc aattttgttt taggtatttt tcttgttttt ctatttctag
    31261 gtttgcggcg ttcttttatt ttaaaatcaa actctgtgat tatacgttat gctgcttttt
    31321 ggaaagaccg agaaatatct gtcggacaga ttcgatcttt gacgccaact aatactgggg
    31381 tgcaaatgat gttgcaagat gagaaagccc catctgtttt catgatgcga aaaaaagatc
    31441 aagtagtcct ttttgatcat tttgaaaaaa acggacccaa atcagaatca attccttatc
    31501 cttatacgaa aaaaagaaaa agctaatttt aaaaatcaga acctaaaaaa acgaattttt
    31561 acaggaaaag aaaagagtga gacgtacggt tcatcccgtt tagcgtctca ctctatttgt
    31621 ttatctattg atgcatacta gtatcggtag tctgaacgtt gcctttcttg acatgggcag
    31681 cttgtgttcc tcgattccct tcgatgatct caaattcgac cacttgattt tcttccaaag
    31741 ttttgaatcc ctcttcttca atcgcagtaa aatgtacaaa aatttcttct gtgtcatcat
    31801 aactgataaa gccgtatcct tttttgttgt caaaccattt tacaattcct gtggtcaaag
    31861 cactcacgac ctttctaaag tctgatcata gccttcatta taaagagtat ttataagtac
    31921 gtcaaataaa ttaaaaacct gtaattttat gttttttttc tgaataaaaa aaggtataat
    31981 tggcatatct taacaaagaa gatattttgt tgaataaagt tcgtgaaaag gggtaatcaa
    32041 atgaagaatg atctgcaaat ttcacaagaa acagagctag aaccgattgt tgaagtggct
    32101 agtaaattag atcttaagga agacgatttg gaactgtatg ggaaatataa ggcgaaaatc
    32161 aatttttctg gaatcaatcg atcaaaggat aacgctgagg ggcatttgat cttagttact
    32221 tccatcaatc caacaccagc tggcgaagga aagtcaacga tcactgtagg gttaggagac
    32281 gcgctcaata aaatcaataa aaaatctgtt attgcattgc gagaaccctc acttggtcct
    32341 gtgatgggaa tcaaaggtgg tgctgcaggc ggaggatatg cacaggtatt gcctatggaa
    32401 gatatcaact tgcattttac tggtgacatg catgctatca caacagctaa taatgcatta
    32461 tctgctcttt tagataatca tattcatcaa ggaaatgaat tgaacatcga tgcccgccga
    32521 gtgatttgga aacgggtagt cgatctcaat gatcgtgaac tgcgtaaagt aattgttggc
    32581 ttgggtgggc caatccaagg tgttccaaga gaagatgggt ttgatatcac agttgccagc
    32641 gaaatcatgg ctatcttgtg tcttgcggca gaccttgaag atttaaaggc tcgtcttgca
    32701 aggatcgttg tcggctatac atttgaccgt cagcctgtga ctgctggaga cttgaaagca
    32761 gaaggcgctc tggcattact gctgaaagat gcaatcaagc cgaatcttgt acaaacaatt
    32821 tacggaacac cagcttttgt ccacggggga ccgtttgcca atatcgctca tggctgcaat
    32881 agtattttag ctaccaagac agcattgaga ttagcagatt acgttgtcac agaggcagga
    32941 tttggtgcag atcttggcgg agagaaattt ttagatatca aagtacctaa cttgaaaaaa
    33001 gcgccggatg ctgtggtgat tgtcgccact gtccgtgcat tgaaaatgca tggcggaatg
    33061 aaaaaagatg aacttaaaaa tgagaatctt gatgctttga aagtaggttt tgcaaattta
    33121 aagcgccata tacgtaatat ggaacaatat cagctacctg tgatcgtcgc aatcaatgaa
    33181 ttcgtaacag atacagacaa tgaactcaca ctgttggagc acttgtgtga aaatcagggg
    33241 attcttgcca agagagcgag tgtatgggca gatggagcag aaggcggcgt tgatttagca
    33301 aaagctgtag tacgattgat tgatagaaaa gaagctaatt acaagccatt ataccgccta
    33361 gaagaaacga tacaagaaaa gacagaaaca atcgtcaaga aaatttacgg aggaaatggt
    33421 gttgttttca gcaaaaaagc gcaaaaacaa atagaagagt ttacgaagaa tggatgggac
    33481 aaactgccga tttgtatggc aaaaacacag tattcatttt ctgatgatcc ttcactgcta
    33541 ggtgcaccgg aagatttcac catcacgatc cgcgaaatca tcccaaaatt aggggcagga
    33601 tttttagtag ccttgactgg agatgttatg acgatgcctg gtttgcctaa aaaacctgct
    33661 gcattgaata tggacgtgac aaatgacgga gaagtgttgg gattgtttta agcttgatta
    33721 taatctacca tcgaaggagc tgtattttga ctacagcttc tttttttaga cgatagggaa
    33781 ttaattttgt ttacactagg atcattcttt ttctttattg tattttgagc tatactaaag
    33841 cataatagga gggtatgatg aaaacgacat ttataagaaa aaccggtttt tacggtatgg
    33901 gtagctcgat tcagctaaga atcaacaatg aaaaacgcag tgttctcagt catgaacaaa
    33961 agataacgct tgatctggaa gtgcccttta cgatgcaggt caccttttat tggctaaaaa
    34021 gccccgttta tacagtgaaa gaagcaaagc ctgcttatgt gatcacaatg aattacttga
    34081 ttcttcaaat ctatccgttt ttatttctag caactgggtt gggggccgtt ttcctacaaa
    34141 atttattttt cagttttttt gcagttattg tgttgattgc gttctttttc tatataaaaa
    34201 accgggcata tttgatcaag gaggaaaatt atggcaaact ctgatctttt tttaatcagt
    34261 ttcaatcgaa tcgaaaaatg gatgagagac gagatgggaa atgcacgaaa tatgggattt
    34321 accgaacttg tcagacgcct agctcaaaga aaacaattaa tgatcagaaa atacgaagat
    34381 gatcttttac agcttgctca gttaaggaat gcgatcgtcc atgatcggat cgcagtcgat
    34441 tttgtcattg cagagccaaa tgattggaca gtcaagcgaa tccagctgat cgaacaagaa
    34501 ctgattcagc ctgaaaccgt gctgcctcga tttgcaaaac gtgtaacagg ttttgaacaa
    34561 gatcttccgt tacttgaact gttaaagata gtagcagaaa aaaggtattc acaatttccg
    34621 ttatacaata aaggaaaatt tgtagcattg attactttac gtaatatcgg gttttggcta
    34681 gcaaaagaaa gccaaaaagg tccggttgat ttaacaaata aacgagcgtt ggatttgatc
    34741 atccaaaacg gaaagtacac aaattatcat tttgttccag ctaaaacaca tatttacgag
    34801 gttgaagcga tgtttcgtga gcaagggact ttggaagccg tacttatcac taaagatggg
    34861 aatcccgatg gtaatctgct cgggatcgta cgacctcgtg atatttataa acaaatagaa
    34921 aaggattgat tttttgttaa ttatttattg gatattcagc gctttgctag taggagtgga
    34981 tcagttggtc aaatggtgga tcactgataa tctagcgctt ggagagacga aaaatcttat
    35041 accaggtatt ttgtcactga atcatattcg aaacactgga gccgcatgga gtatgctaga
    35101 agggaagatg tggttcttta ctatagtgac actcattgca gtcgttgtga ttctcacatt
    35161 gatgattaaa aatcgtgaaa aaggtaatcg ctggttcatg attggattga gtttgattct
    35221 agctggtgcg attggcaatt ttatcgaccg tattcgtctt ggctatgtag tagatatgtt
    35281 ccagacagat tttatgagct ttccgatttt taacgtagca gatgtgacac ttgtatgtgg
    35341 agtgatttgt atgttaattt atattatttt agatgaaaag gatcaaagaa aaaaatgaca
    35401 gaacttaaaa cgacgattca ccatgaatct ggtaggatcg ataaagtatt gactaattta
    35461 ttcaccgatt acagtcgttc gcaaattcag ctgtggctga aaaatggagc ggtttctgtc
    35521 aatggacagc cagtcaaagc aaattacaaa gtaaaacaag gagacgagat tactgtcttg
    35581 atccccgaac cagaaacatt agatgtggtc ccagaaaata tcccgttgac gatcgtttac
    35641 gaagatgaag atgtagcggt gataaacaaa cctcaaggaa tggtcgttca tccttcagca
    35701 ggtcatacgc atggcacaat ggtcaatgcg ctgatgtacc atatcaagga tctttctacg
    35761 atcaacggcg ttattcgtcc aggtattgtt caccgaatcg acaaagatac ttccggactg
    35821 ctaatggttg ccaaaaatga ccaggcacac gaatcattag ctcagcaatt aaaagataag
    35881 acctctttgc gcaaatatgt tgctttagtc catggagaga ttcctcacga aaaaggaacc
    35941 atcaatgcgc caatcggccg ttcgaaggaa aaccggaaaa tgcaggcgat aagagaagat
    36001 gggaagtcgg ctgtcacaca ttttactgta ttggaacgtt tcgaaggatt tactctggtt
    36061 gaattaaaac tagaaaccgg aagaacacac cagatccgtg tgcatatgaa gtatatcggt
    36121 tatccgttag ctggagatcc agtatacgga ccgaagaaaa ctttgaaagg caatggtcag
    36181 tttctccatg cgcaaatgtt aggttttacg cacccgacta caaacgaaaa gatgatcttt
    36241 gaagcacctc ttccagaagt ttttgaaaaa actttagaaa aattaagaaa aaacattgca
    36301 ttttaattta gataacggta tagtaattaa caacgaaagc ataaaactta atagaattcc
    36361 tttaagttta gtcccgtgag gctaagaagg gtgggagcaa ggatacgtat ggtgcgccat
    36421 aagtagattg ctcattgaca tagtatagct atcagtgtat cagtatacgc tcagtctaaa
    36481 cattcctcct gtcctcttag ggcaggagtt tttttatgca aaaaagtagg gggaatgaaa
    36541 atgcaagcaa aagaagtagt agatcaggta accatgaagc gagcattgac tcgaattact
    36601 tatgagatta tcgagagaaa ccatagtatc caagatatcg tattggtagg tatcaaaaca
    36661 cgaggaatct acattgcatc aagaattgcc gaacgcctga agcagctaga agatatcgat
    36721 attcctgtag gagaactgga tatcacttta taccgagatg acaaaaaaga aaatccagaa
    36781 gaaccggaac ttcattcttc tgacatccca gtttccttag aaggaaaaga agttattttg
    36841 attgatgatg ttttgtatac aggacgaacg attcgagcag caatggacgc agtaatggat
    36901 tttggcagac caagaaaaat ttcattagca gtcctggtcg atcgtgggca tcgagaattg
    36961 ccaatccgtg cagattatgt tgggaagaac attccaacat ctcgggcgga agagatttta
    37021 gtagagatgc aagagcttga tggacaagat cgcatcatga ttttgaaaga ggaagattag
    37081 aggagagaga aacatgtcgg agaaaaaagg atttcataat gatgacgtgg tgttagatat
    37141 ccacgatcgc ccaaccccag gacattgggt aggattaagt ttacaacact tgttcacgat
    37201 gttcggagca accgtgttag tccccatctt agtaggtatc gatccaggta ttgcgctagt
    37261 cagttcagga ctaggtacat tggtgtactt atttactact aaaggaaaga tacctgcgta
    37321 cttaggaagc agcttcgctt tcattgcagc gatgcagctt ttgatgaaaa cagatggtta
    37381 tccagcgatt gcacaaggag cagtcacaac tggattagtc tatttgattg ttgcgctgat
    37441 catcaaaaag atcggttcag catggctgga taaaatcttg cctccaatcg tcgtcggtcc
    37501 agtcatcatg gttatcgggt tgggacttgc ggcaaatgca gcaacaaatg ctatgtataa
    37561 tcagggcacg tatgatttca agtatattgc tgtagcgctg atcacattag gattaacaat
    37621 tttcttcaac atgtggctaa gaggttttct cggattgatt ccgattttac taggtattat
    37681 tagcggatac atcgtagcct tgctgtttgg aatcgtggat actaagccaa tcgtagacgc
    37741 tgcctggttt gctgttccta actttgagat tccctttgta caatatgaac caaagctata
    37801 tcttggagca atcacaacaa tggcacccat cgcttttgtg acgatgacag agcatatcgg
    37861 ccatcttatg gtattgaata aactaactaa acgtaatttc tttgaagaac cgggtcttca
    37921 taagacgttg acaggtgatg gattagcaca aatcgtggca gcctttgtcg gaggtcctcc
    37981 tgtaacaagt tacggtgaaa acattggtgt tctggctatc acacgtgtcc atagcgtgtt
    38041 tgtgatcggt ggagctgcag tatttgcagt cattttagga ttcgtaggaa aattaagcgc
    38101 tttgatttta agtatccctg gaccagtcat ctcaggaatc agttttatct tatttggagt
    38161 aattgccgca agtggaatga agattttaat cgagaacaaa atcgattttg ataaaaagaa
    38221 aaatctgttg attgcttcag ttatcttagt agtaggaatt ggcggactcg tctttgaagt
    38281 aggcacattt acattgtcgg caatggcatt agcaactgta cttggcatca ttttaaatct
    38341 tatcttacca gaaacttcac gaagcgaaga aaactaagga ggaaacatta tgatcatcaa
    38401 atcagaacgt atcagcttaa agcatttatt gaccgttgag gcacttactg atcaagaagt
    38461 tatgggcttg atccgcagag ggcaggaatt caagcaaggt gctaattgga ctccgcaaaa
    38521 gcgccagtac tttgctacaa acctcttttt cgaaaatagt actcgcacac ataaaagctt
    38581 cgatgtagcg gagaaaaaac tagggctaga agtgattgaa tttgaagcaa gcacaagctc
    38641 tgtccaaaaa ggggaaacac tatacgatac agtactgacg atgtcagcat taggcgtaga
    38701 cgtagcggtt atccgtcatg gtgatgagaa ctattatgac gaactgatac aaagtaaaac
    38761 aatccagtgt tcgattatca atggaggaga cggaagcgga cagcatccaa cccaatgctt
    38821 gcttgatttg atgaccattt atgaagaatt tggtcatttt gaaggtctga aagttgccat
    38881 cgtaggtgat atcactcact ctcgcgtagc taaatccaat atgcagatgt taaaacgtct
    38941 aggcgcacaa gtattcttct caggtcctag agaatggtac gacgatgaat tcgaagtgta
    39001 cggacattat atgccattgg atgaactact agatcaagtt gatgtcatga tgatgcttcg
    39061 ggtgcaacac gaacgccatg atggaaaaga aagtttctca aaagaaggct accatcagga
    39121 acatgggtta acagttgagc gtgcgaagaa gttgcaaaaa catgcaatca ttatgcaccc
    39181 agcaccagtt aaccgtgatg tagaattagc tgattcatta gtggaaggat tacagtcgcg
    39241 gatcgttcag caaatgagca atggagtatt cgtcagaatg gcaatcttag aagcagtgct
    39301 aaatggaaaa gcgtaattat aggaggatga aaaaatgaaa acactcatta aaaacggtca
    39361 gatcaataca cgaaaaaata tgacgacacc agcagaaatt tggatagaag atgggcgaat
    39421 caaagcaatc ggtacaggat tctcagaagc cgaatttgat gaagtgtttg atgcaaaggg
    39481 acaattgatc acacctggtt tagtggacgt ccatgttcat ttgagagagc caggatttac
    39541 atacaaggag acgatcgaag ctgggactcg ctcagctgct cgtggcggtt tcactactgt
    39601 atgcgcgatg ccgaatttaa atcctgtacc tgatacggca gaaaagctga gacaagtata
    39661 tgacatcatt agaaaagatg cagttgtgaa ggtattgcag tatgcgccaa tcacagaaaa
    39721 tctgcgaagt gagaaattag ttgatcaaga agcactgatt gaagaaggtg cttttgcttt
    39781 cacaaatgac ggtgtaggtg tccaaacagc tgggacgatg tatctggcta tgaaagaagc
    39841 tgcaaaaaac aataaagccc tagtagccca tacagaagat gaatctttat tatttggcgg
    39901 cgttatgcac gctggcaaga aggcagaaga acttggtctt cccgggatct taagcgtaac
    39961 cgagtcttct caaattgcta gagatctgtt gctagctgaa gcaactggtg ttcattacca
    40021 tgtttgccat gtttctacta aagaaagtgt ccgtgtaatc agagacgcaa aaaaagcagg
    40081 aatccacgta acagcagaag tttcacccca tcatcttatt ttgatcgacg aagatattcc
    40141 cgaagatttc ggcttttgga aaatgaaccc gccgctaaga ggacgggagg acagagaagc
    40201 tttgatcgaa ggcttgcttg acggtacgat cgattgcatc gctacagacc atgcccccca
    40261 tggattagaa gaaaaaagcc aaagtttcat gaaatcacca tttggtatcg taggaagcga
    40321 aacagcgttt caactgatct atactcattt tgtagaaact ggtcgattca cgttggaaca
    40381 agtgatcaat tggttggcag ttaaaccggc tgagattttt ggtttgaatg caggaacgct
    40441 aacggttggt gcccccgctg atgtagcagt ttttgatatt acacaaactt gcacgatcga
    40501 taaagaagac tttttatcaa aaggggagaa tacacctttt atcggctgga aagtaaaagg
    40561 cgaaacacaa atgacttttg tcaacggaaa acttgtgtgg cagaagggag aataaaagta
    40621 tggaacgttt gcttatatta gaagatggaa cagttttcga aggaaaggct tttggtgcag
    40681 atgctagtgt cttcggagaa atcgttttta caacaagtat gactggttat caagaaacta
    40741 tcactgatca aagttttaac ggtcaaatca ttacatttac ctatcctatg gtgggtaatt
    40801 atggggtaaa tagagatgat tatgaatcga tttctccaac atgcaaagga gtagtagtaa
    40861 aagaacatgc acgtgtagct tcaaactggc gaaaccaaat gacgctagat tcatttttga
    40921 aacaaaaagg aattcctgga atatcaggta tcgatacgcg cgcccttact cgcaaaatcc
    40981 gtgaagtcgg aacgatgaaa gctagtatag ttgatgctgg agattccttc gaacatgcat
    41041 ttgatcagct gaaagcaacc gttctagcaa ccaatcaagt gcagcaagtc tcaactaaca
    41101 aaccttatcc aagtccagga acaggtcgga aagtcattgt cattgacttc ggcttgaaac
    41161 atagtatttt acgagaattg agtaaaagaa actgtaacct cacagtactt ccctataaca
    41221 cagatgccga gacgatttta gcgctatcac cagacggagt catgctgacg aatggacctg
    41281 gtgatccaaa agatgtgcca gaagcaatcg aaatgatcaa aaaaatccaa ggaaaagtac
    41341 cgatttttgg tatttgtctc ggtcaccagt tatttgcttt agccaacggt gcagatacct
    41401 ataaaatgaa attcggacat cgaggattaa atcacccggt acgagaagta gcgaccggaa
    41461 gaattgattt tacttctcaa aatcatggct atgcagtcga tgaagccacg attgatccag
    41521 aaaaactcat cgtaactcat gtagaagtaa atgacggaac agtagaagga gtcaaacaca
    41581 gagattatcc cgctttcagt gtgcagtttc atccagatgc tgcccctggt ccgcatgatg
    41641 cactacattt atttgatgaa tttatggaaa tgatggacgc aggaaaggag cagtaaacag
    41701 atgccgaaac gtacagatat caagaaaatt atggtgattg gatctggtcc gatcatcatt
    41761 ggtcaagccg cagaatttga ctacgcagga acacaagcct gccttgcctt aaaagaagaa
    41821 ggatatgaag tggttttagt aaattctaat cctgcgacga tcatgacgga taaagaagtg
    41881 gcagatcaag tatatatcga accgatcaca cttgaatttg tatcaaggat tcttcgaaaa
    41941 gagcgtccag atgctctcct tccaacttta ggcggacaga caggcctcaa tatggcaatg
    42001 gagctggcag catcaggtat tttagaagaa ttgaacatag aacttctagg aacaaagctt
    42061 tctgcgatcg atcaagcaga agatcgtgat ctattcaaac agttgatgaa agaattgaat
    42121 cagccaatcc ccgagtctga aatcgtcaat acagtagaag aagctgtaga ttttgcaaat
    42181 aaaatcggct atcctgtcat cgttcgtcca gcctttactt taggcggaac tggtggaggt
    42241 atgtgtgaaa atgaagaaga attacgtatc gtatcagaaa atggattaag tctttccccc
    42301 gctacccaat gtttgattga acgaagtatt gcaggtttta aagaaattga atatgaagtg
    42361 atgagagatt cggctgataa tgcaatcgtt gtctgcaata tggaaaattt tgacccggtg
    42421 ggtatccata caggtgattc tatcgtattt gcccctagtc aaacattgtc tgaccatgaa
    42481 tatcaaatgc ttcgtgatgc ttctctttcg attattcgag cacttaagat cgagggaggc
    42541 tgtaatgtcc aactcgctct tgatccacat agttttaatt attatgtgat tgaagtcaat
    42601 ccgcgtgttt ctcgttcatc tgcgttagca agtaaggcga ctggctatcc gatcgctaag
    42661 ctagctgcta aaatagcgct tggattaaca ttagacgaaa tgaaaaatcc tgttacagga
    42721 acgacttttg cagaatttga acctgcatta gattatgttg tagctaaaat tcctcgctgg
    42781 ccgtttgata agtttgaaaa cggagaacgg cttttaggca cgcagatgaa agcaactggt
    42841 gaagtgatgg ctatcggtcg gaatatcgaa gagtctttat tgaaagctgt gcgttcttta
    42901 gaaatcggta cacaccatat cgaacttcct gagttggcag acatcacgga cgatgaccta
    42961 atccaaaaag tggttcgagc acaagatgat cgcctgtttt atgtagctga agctattaga
    43021 agagggtaca ccatagaaga attagcggat ttgacaaaaa tcgatttatt ctttttagat
    43081 aaactgttgc atatcgtaga aatcgaacag cagttgaaac agcagccgtt ggatgacaag
    43141 atattgaaaa cagcgaaaca aaacggtttt acagacaaaa aagtggcgga gctgtgggaa
    43201 ataaacgaag aagccgttcg ccagatgaga aaagaaaatg atttgattcc tgtatataag
    43261 atggttgaca cttgtgcagc ggaattcgaa tcaaaaacac cttattttta tagttcctat
    43321 gaatatgaaa atgaaagttc acgatcagaa aagccctcaa ttctagtact gggatctggt
    43381 cctatccgta tcggacaagg ggttgaattt gactatgcga ctgtgcattc tgtcaaagca
    43441 atccagcagg caggatacga agcaatcatc atgaacagca atcctgaaac agtttcgact
    43501 gacttttcta tttcggataa attatatttt gagccattga ccttcgaaga tgtgatgaat
    43561 gtgatcgagc tagaacagcc gataggagta atcgttcaat ttggtggtca gacagcaatc
    43621 aacttagccg aacctttgac tgctgctggt gtaaaaatct tagggacaac gatcgaagac
    43681 ttggatcgtg cggaaaatcg tgatttattc gagcaagcgc tgaaagagtt ggatattcct
    43741 caacctttag gggatactgc tacaagtaaa gaagaagccg tggttatcgc ttcgagaatt
    43801 ggttatccag ttcttgttcg tccaagctat gtattaggcg gacgtgcgat ggaaatcgtt
    43861 gaaaaccagc gagatttaga agattacatg gaacatgctg tgaaagcatc tccagaacat
    43921 ccagtactag tggaccgtta cttgcttggt ggtgaatgcg aagtggatgc gatttgtgac
    43981 ggtgaaacag tactcatccc agggatcatg gagcatattg agcgtgctgg tgtccattca
    44041 ggggattcaa tggctgtgta tccacctcaa acattatctg aatcgatcaa gaaaacgatc
    44101 gaagattaca cgatccgctt agcattaggt ttgaattgtg tcgggatgat gaatatccag
    44161 tttgtcatcc atgaaaatga ggtttatgtg atcgaagtca atcctcgagc aagtcggact
    44221 gttccattct taagtaaggt gacaggaatt ccaatggcac aaattgcgac gaaagctatt
    44281 ttgggagaga aactaaagac acttggttat gaaaacggac tttatccaga aacaaaagaa
    44341 gttcatgtta aagcacctgt cttttctttt acgaaattgc agaaagttga tacttactta
    44401 gggcctgaaa tgaaatcgac tggagaagta atgggatctg acagacaatt agacaaagct
    44461 ttgtacaagg cattcgaagc ttctggatta cacttgcctg aatatggagc cgtattattt
    44521 acgattgcgg acgaaacaaa agaagaagca ctagaattag ctaaacgttt tacagaaatt
    44581 ggctatagtc tgataggaac gaaacataca gctgcttatt tcgaaaaaaa tggtctggtc
    44641 gttgaaacag tagcgaaaat ttctgaggaa gcagcagaaa aaaatgtagt cgacttaata
    44701 agagatggta aaacacaagt ggttgtcaat acgattgata aagatcgggg aaatgcttct
    44761 aaagacggtt tcatcatccg aagagaagca gttgaacatg gtattccatt gtttacttct
    44821 ttagacacag cagatgcgat cgttcgagtg atggaatccc gtgctttttc aactaaccct
    44881 atttaacggc gagcacatgt taagacaaaa agacgaaaaa ccgttgcagg aggggacaga
    44941 tgaaacaaga aatgatggaa atcgttcagc aaaaacagct ggcaccaaag atttttgaaa
    45001 tgacattgaa aggtgatcta gtaaaagaaa tgcagcaacc aggacagttc ttgcatattc
    45061 tcgtgccgcg tgcagacttg ctattacgcc gaccgattag cttgaatcat atcgaccacg
    45121 aaacaaatac ttgccgtatc atttatcggg tggaaggtga ggggactaga gtcttctcta
    45181 ctctttctgc cggagaccat ttagatgtca tgggtccttt aggcaatggt tttgatttgt
    45241 ccaatcttca tacaggagac gaagtattta taatcggtgg aggaatcggt gtccctcctc
    45301 tatatgagtt atcaaaacag ttagtcgaaa aaggaattaa gccaacgcat tttttaggtt
    45361 ttgccacaca tgaagtcgtt tattatgaaa aagaatttaa tcgtctagga gaaacaagaa
    45421 tcgctactga cgatggaaca tacggtatgc acggaaatgt cggtaattta ttgttaggaa
    45481 cgaagacgga acctacagct gtttttgcat gtggttcaaa cgggttattg aagacagtgg
    45541 aacagctgtt ttcttcgcat caaaatgtcc agttatcatt agaatcccgc atggcatgtg
    45601 ggataggggc ttgctatgct tgcgtttgtc atacgccgga agatgaaaca ggaacaaaaa
    45661 gtgtgaaagt ttgtgacgaa ggaccgattt ttaaaatagg agaggtaatt atatgaatga
    45721 gaatccttta gcggtcaaac ttcctggcct agaattaaaa aatcctatca tgccggcaag
    45781 cggctgtttc ggttttggca aagaatacgg aaaatactat gatttgaatc agcttggatc
    45841 gatcatggtc aaggcaacga ctccaaatgc ccgctttggc aatcccacgc caagagtggc
    45901 ggaaacacca tctggcatgt tgaatgcaat tggtttgcaa aatcctggtc tagacgttgt
    45961 tatgcataaa cttcttccag aattagagga gtacaatgag ttacccatca tcgcaaatgt
    46021 tgctggtgct tgtgaagaag actatgtaga agtttgtagc aaaattggcg aagcaccaaa
    46081 tgtgaaagca atcgagctga acatttcttg cccaaatgtc aaacatggcg gaattgcttt
    46141 tggaacagat tctgatgttg cttttcaatt aacacaagca gtaaagaaag tttcttctgt
    46201 tcctgtctat gtcaaattgt ccccaaatgt aacagacatc gttccaatcg ctcaggcaat
    46261 cgaagctgga ggggcagatg ggtttacgat gatcaatact cttcttggta tgcggattga
    46321 ccttaagaca agaaaaccga ttttagccaa tcaaacaggt ggactctccg gaccagcaat
    46381 caagcctgtc gctattcgtt tgattcacca agtagcaagt atatccagcc ttccaatcat
    46441 tgggatgggc ggcgtgcaga cagtggatga tgttcttgag atgtttatgg ctggagcaag
    46501 tgctgtagct atagggaccg cgaatttcac cgatccgtat atctgtccta aactaattaa
    46561 agagctccct gtgcgaatga gcgaactagg catcgaatcg ctggaaagat taatcaaaga
    46621 agtgagagag gaacgtgaac gatgaatcaa ctagaccaac gaccaattat tgcattagat
    46681 ttttcaacta ggcaagaggt agaagacttt cttcgatttt tcccgaaaga agaaaaattg
    46741 tttgtgaaaa tcggaatgga attgttctac caagaaggac ctgaaatcgt acgttatcta
    46801 aaagaggctg gtcataatgt atttttagat ctgaaacttc acgatattcc gaatacggtc
    46861 gaaaaagcaa tgcggggatt ggcaaagctt ggtgttgatc tcacctgtgt tcatgccgct
    46921 ggcggtattc gtatgatgga ggcggcaatg cgaggattag aagaggggac tccagaaggt
    46981 ggaaaacgtc cgctacttct agctatcact cagcttactt ctacaagtga agaagaaatg
    47041 catgccgatc aactaatcga agtttcttta gaaaaaagtg tgatccatta tgctagctgt
    47101 gccaaaaaag caggattaga tggtgtggtt tcttctgctt gggaagtaga agcgatcaaa
    47161 gaaatggcag gagacgaatt tgtttgctta actccgggta taagaccaga aggaacagtc
    47221 gcaggagatc agacaagagt agtcacacct tctcaggcaa aaaagatcgg ctcaactttt
    47281 atcgttatag gtcgaccaat cactcagtca actaatcctt ataaagcata tcaaacgatt
    47341 caaacagaat ggagctaacc taaaatgaat gttgaacaaa gtatcgcaaa agacttatta
    47401 gaaattgaag cagtattttt aaatccaagt aatcctttta catgggcaag tggaatcaaa
    47461 agcccaattt actgtgataa ccgaatcacg atgagctatc caaaagtccg aaaagagatc
    47521 gcaaaaggat tagcaagcaa aatcaaagaa gcttaccctg aagtacaagt gattgctgga
    47581 actgcaacag caggaatccc tcatgccgca tgggtagcgg aaatactgga tttgccaatg
    47641 gtctatattc gaagtaaggc gaaagatcat gggaaaggta accaaatcga aggacgtata
    47701 tttgaaggtc agaagatggt tgtgatcgag gatttgattt ctacaggcgg aagtgtcttg
    47761 gaagcagctg aagcagcaaa aagagaaggt gcggatatcc ttggtgttgc ggctatcttt
    47821 acgtatgagt tgccaaaagg aaaagcaaat ttcgaaaaag ccgaaatccc tcttatgacg
    47881 ttgaccaatt attctgtatt gattgaagca gctttagaag atcgttatat cgatgagcaa
    47941 gaattaactc tgctaaaaga atggaaaaaa gatcctgaaa attggcagat ttaagcgcgc
    48001 ttgatgaaga tagagaaatc cgatttgtgt ctccagaatc atcaattgga atagcaaaaa
    48061 tccaaaaact gattgcgaga agcatctttt catcaggtat gaaaagaaac ttctatcgtg
    48121 atcagttttt gattttttta aaattcattt ttctaagact ggttagcaga aagaagagaa
    48181 taatctcttc tcttttttct attatatata gattttctct attttttggt aagggagctg
    48241 tttcaaaaat ggtaaagagc ggaaatgttg cgaatatcag ttatttaatg atatgatatt
    48301 cggaaaaaga aatctcaaag ggaggtaact tttatggggt tgaatgaatt tattttagga
    48361 ttgaacaatt tagagacgat cacaagggca cctggatttt ttaaatatac agagcataca
    48421 gttgcagcac attcgtatcg tgtagcatct attgcgcaag ttctaggaga cattgaagaa
    48481 gcgaatggac aaccgatcga ttggaaactg ctctatgaaa aatcattgaa tcatgactac
    48541 acagagcgtt ttattggtga tatcaaaacg ccggtgaaat acgcaagcaa agagttaaga
    48601 gggatgctgc aaacagttga agaaaagatg acggacgaat tcatcaacca agagattcca
    48661 gctgagttcc aagaagttta tcgccgtcgg ttgttcgaag gaaaagatga aaccgtagaa
    48721 ggagaactat tagccatttc agataaggtt gaccttcttt atgaatcttt tgatgaaatc
    48781 atcaaaaata acccggaaaa agtttataag gagatgttca ttgaggcagt gactacgatt
    48841 aaaagctttg aacatcggcc ttctgttcag tatttcttta aagagatatt tccggaacta
    48901 ttgaaccagg aattttatgg aaaagaggcg tttttagaaa ccgtctcaac gattgtaaag
    48961 aaagaagaaa aataaaaatg atatgagatg cagggggaag tatagttgaa aaagaatatg
    49021 gatgtcgaat ggggctattc tggagacaaa ggtccggaaa attgggcgag tttatgtgat
    49081 tggtttgcga gaggtgcgga atttccattg cagtcgccta ttcatttgac caagggggaa
    49141 gaaaccaaaa tagaaggaga cacgctttcc tttcattatc aaaagcaacg gtttactgac
    49201 aaggaattca aaaacacgat tcatttagta ccttttgacc aactcagtta tgtagagttc
    49261 aagaaagaac gctattattt gacagatatt catttccact tgccttctga acatatcatc
    49321 aacggaaaac aggaagatat tgaatttcat tttgttcata tgaacagcaa aaaagagaat
    49381 ctagttgtgg gtgtcatgta ccagctaatg gtacaggaag gttggctgta taatcaagaa
    49441 aatggggaaa gttggaactt agaaaaacat gagcattggg taaatccgga tgtgttcttt
    49501 ccggagcaaa aaagccattt ccattatatt ggctcattga caactccgcc taccagcggg
    49561 ccaatccaat ggtttgtaat ggatcatgta ggaaaactga accaagagtt tcttctcccc
    49621 ttcgatggtc agtatgctcg accgaacaac cgaccaatcc agcccttgaa tggaagagaa
    49681 atttattatt tcgaggagtt tgagcaatga atattcagca gatgaaatac gtggtcgctg
    49741 tagcaaacaa tggtagtttc agagaagcgg caaagaaaat gtttgtctcc cagccaagtc
    49801 tttcagctgg tatcaaagaa ttagaaaccg agttagggat cacgctgttt actcgaacga
    49861 atcgaggggc atatttaaca gaagaagggc aagacttctt aaatcgtgcc gagaaaatat
    49921 tggtccagct tgatacatta gaaactcact atctaacgag tgaaaagaga gacaattttt
    49981 cgatcgctgc ccagcattat gatttcttag gtcctttgac cgcacagcta ctcgaggaat
    50041 tcaaagacga gatcaaacaa tttcggatca tggagacgac aacagcgaga gtgatcgaag
    50101 aagtcaaaga gcagcacagt gagatcggca ttatttatat gaatgagcat aatcaagctg
    50161 gtatcagaag atatttacaa caaggagagc taagtggccg cagcattgga aaattcaaaa
    50221 cgcatatttt tttaaggcaa ggacatccat tagctgaaca cgcagagatc accaaagaag
    50281 aattgttatc ttatcctcaa gttcgtttta cacaggatgg aaataatttc ccgtattttt
    50341 atgaagacct tatcgagatc cctgatcaag aaacagtgat ttatacgagt gatcgaggta
    50401 ctttgatgaa tatcgttctt gaaactgatg cttacgcttc aggatctggt attgtgattg
    50461 gagggattcg cgagcacttg cggttaatcc ctttggcaga aggcgaacta aatgaatttt
    50521 atatcattta ttcggcgaaa cgtccgctca gtgaaatcgc tcaaagattt atcaaagagc
    50581 ttactcggtt actggaccag cagagctaag aaaagcagat gagaacgctg tttattgaac
    50641 accgttgatc aaaaaagagg ggttgtgaca gaattttgtc acaactcctc tttttgtagc
    50701 aaacaattta taactaattg ctgttgtggg aaagtacgca aatatgattc tagaaacact
    50761 cattgattac ctgttttgtt tcatagctgt aattttttct tcttctggtg tcacgataat
    50821 cgtcttttgg ttttcataaa taacataacc aggcttggcg ccatttggtt ttcggatatg
    50881 ctttacctgt acaagatcaa ccggcacttg ggcagagtat cggtattttg aaaaataggc
    50941 agctaactcg gcagcttctg taattgtttc atctgagggc ttgtcacttt taatgatgac
    51001 gtgcgaaccg ggtatatttt ttgcatgaag ccaatagtcg gtttttttag ctgtcttcat
    51061 tgtcagctgg tcattttgaa gattgttttt accaacaaga atgagcgttc cgtcagaaga
    51121 aaagtattga tcaggctgag atggtttctt acgtttttgt ttttttaagt tcttcttttt
    51181 caggtatcct tctgaagtca attcttcttt aattgcttcg atatccattg ggccggcaat
    51241 ttccagttga gataagacag attcaagata ttggatttca tctttggctt cttggatttg
    51301 ttcgccaatc agcttaacag cgttcttcag cttttggtat cgatggaaat atttctgtgc
    51361 attttgattt ggtgtcaatg ccggatctag tgctattttg attggccgat cttcttcata
    51421 ataattaggt aaaacaactt cttttgcgcc gcgtggtact tgtgtcagaa aagtagtaag
    51481 tagttcgcca tttctacggt aattctcagc gttttcggat tctttgagtg tttgttcacg
    51541 ttttttcaat ttatttttat ttcgttttag ttcattttcg atttttcgaa tcagttctcc
    51601 gccttgttgt tttgcccgat ctttctctgc tttttcgtga taatagctat ctaataattg
    51661 actgagtgtt ggataagctg ttacagcaga tgcctgttcg gacaatacct gataagcaat
    51721 cggtgtgaaa aaatctttat tttccgtttc gtaaaaagta ggaataggct gatggctgat
    51781 ggctgagaaa aattcgtccc atacaaccat cttttcatta ggacgttcca gtaatctttt
    51841 ggtcagctct tgagcgatat cgaaaccgat tccctgaaac tgacgttgga ttgcttttgg
    51901 atctagttca gtttgggaaa gccgctgaaa gattttttct ttctcggagc taaatgggtt
    51961 caagacctct tgttttggcg gagcaacata ttctacacca ggaagcaaag aacgataagt
    52021 gttttgagag cttccaatgt gtttgatcgt atccagtatt tttccagttt ctcgattaac
    52081 taagacgatc gtactatgac gtcccattaa ttcaacgatc aatatgatat tttgtagatc
    52141 acccaactcg tttcgtttag caaagtggaa atggatcact cgatcatttt cgatttgttc
    52201 gatgtcttct aaaatagcgc catccaaata ttttcgaagc atcatcacga agttaggcgg
    52261 attatcagga ttctgataat cgatttgggt aatctgtaca cgagcataac ttgggtgcgc
    52321 agataataat aatcgatggt ttttgccgcg tgaacgaatc accagcacga tttcattttc
    52381 ataaggctga tggattttcg agatccggcc agatagtaac gtttcacgca attcgttgac
    52441 catcgcgtgg gtaaatacac catcaaagga catggttcat tcctcatttc ttttcataag
    52501 tatattcatt ataacgaaaa aaagaagaaa aaccaatttg gccgaatagt aaagcaaaag
    52561 tctagaaaaa tcttggacta attacataaa aaacagtgtt tctatcgttg aaatccaaat
    52621 ttttaggtat aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa
    52681 gtagttaaat cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat
    52741 ccaagtaagt attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt
    52801 gcaatgtttt tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg
    52861 tccataccaa ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa
    52921 caaagccggg cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg
    52981 cttgatgttt ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat
    53041 ccgatcgttt atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg
    53101 acatttattt tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga
    53161 ataactgatt ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc
    53221 aaacaattag aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct
    53281 aattgagaca ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga
    53341 acaagtgatg tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa
    53401 ttcaaaaatt cgatttgact taaatcaatt cttaaggaaa gggtgacctg taatgaactt
    53461 atcaaaacgt atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt
    53521 attaatggtg agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat
    53581 ctatgctaga catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta
    53641 aagacaatga ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata
    53701 ataaataaaa agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag
    53761 ttgtagtaat tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg
    53821 aaaaagaaag tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat
    53881 acgtgagcca tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag
    53941 aaggattaaa agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga
    54001 gcaagctagc cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa
    54061 tcgcaacacc tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag
    54121 gtgctgtaac ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta
    54181 atgtaacagg tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt
    54241 tgttgccaaa tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat
    54301 accaagtagc acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg
    54361 cggtgccttc cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt
    54421 ttatctatgt tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag
    54481 caaacaaagc aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac
    54541 tagcaacaat cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag
    54601 aacttgcgag tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg
    54661 atacagtgat caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga
    54721 aggaaaaagc caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca
    54781 aatgatcgta tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt
    54841 catgacttat cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg
    54901 tgctgctgtc tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt
    54961 aggcgtaggt gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa
    55021 gataccggtt attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt
    55081 tgtgatgcga acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca
    55141 tcgctttggg ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat
    55201 cttgattgct gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac
    55261 aggagacaac gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt
    55321 aggtttaact ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga
    55381 cggatatgca gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat
    55441 catcggtgaa gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt
    55501 cggcagtatc atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac
    55561 ttatctgaag ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa
    55621 gaaagcactg aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg
    55681 acaatcaaaa atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg
    55741 gatgaaatta accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt
    55801 gctggaaaaa gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg
    55861 atctctttta aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt
    55921 tctcgagttt tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat
    55981 ttgctacttg cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat
    56041 caaaaagaag attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg
    56101 gatactccag cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg
    56161 acgatcacag ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa
    56221 acagctaaag cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt
    56281 ctcatgatca cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc
    56341 caaaaaggga aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc
    56401 gaccttttgc agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag
    56461 tagaaggact tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata
    56521 aacaattaaa catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa
    56581 atagaatatt ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc
    56641 tagacgtttc gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac
    56701 aaaggaggaa taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg
    56761 cttctcacag tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg
    56821 cggtgtattt agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc
    56881 gaacttttga tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt
    56941 atctggatga ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg
    57001 aaaagggtcg tttgattggc tataatactg atggtgcggg cttcatgcaa tccgtgcaag
    57061 aagctgggat ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa
    57121 aggcagtcac tgttcaagca gcgttagacg gcgctgagga aatcgtagtt tataaacgaa
    57181 acaaccaaac attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt
    57241 gtaagataaa agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa
    57301 gctgtcttct agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc
    57361 tcttagatat aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta
    57421 gagaaacacg tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg
    57481 gcatgttgat ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga
    57541 tccatgtgat tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct
    57601 cgcataaacg gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt
    57661 tttattaaat tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta
    57721 tcagtaagaa acgaggagga accacaatga gtcggatgga tcgctataag gacatccata
    57781 agaaggcgaa acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac
    57841 gaacgcgtat ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat
    57901 ttgaacattt gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac
    57961 aacaaaaaaa taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa
    58021 gaaagcgatc gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag
    58081 gtatctcatt ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga
    58141 cttcagaaac ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg
    58201 gaagtgatac acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg
    58261 atggtccttc ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc
    58321 caggggtagg acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc
    58381 gtcaaacttt agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca
    58441 aatcgtttga aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa
    58501 aagatctgaa tctggatggt gtagatatta aaaaaggggg ccagaagatg gatggacatg
    58561 ttcttttgca gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac
    58621 gccaacagca agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc
    58681 gtgcacctta tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt
    58741 tcttgacgaa aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg
    58801 tgccagtaga cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa
    58861 ttgataatca aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa
    58921 gatcaagaag aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa
    58981 aaacgctttc ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct
    59041 gaacttcaaa aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga
    59101 agcatcaggt atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga
    59161 agaagcaaaa aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt
    59221 tgcagatacg ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt
    59281 catccagaca agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg
    59341 taagattgag atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga
    59401 tcgatttggc atcaactggc agattaacca agagtgacag ctaaagatga atattttctg
    59461 gaaacatcaa gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg
    59521 actatacggt atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc
    59581 cgcaaaaaat taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct
    59641 tcacaagcaa aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat
    59701 gaaatcagcg atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg
    59761 gtagatatgg ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat
    59821 ttgcctattt tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt
    59881 tcgatcgtaa aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat
    59941 cgagaaggtg aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa
    60001 aagaactatt tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt
    60061 aagaatctcc aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag
    60121 aacgctgatt ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga
    60181 aaagggattg atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt
    60241 tatcagctgc aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt
    60301 ttaattacga caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa
    60361 acccaagaag agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac
    60421 ggacggattc ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg
    60481 ctatctagtt tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt
    60541 gatgctgctc aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt
    60601 tacattacag aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt
    60661 ttaggaatag aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg
    60721 aaaaaagttc aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc
    60781 tttgcgaagc tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg
    60841 gctatgtttg ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa
    60901 ttaaagctaa aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc
    60961 atcaagtcaa aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca
    61021 gaagtagaac tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga
    61081 acgttgatca cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa
    61141 attctcaaag aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat
    61201 cttaagcaga agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga
    61261 atcactcttt gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg
    61321 cgctgggaag ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta
    61381 gagaatatca aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag
    61441 gtagactttt cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac
    61501 gatcaattag gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc
    61561 gctacatgct tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag
    61621 ctgactaaaa cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa
    61681 ggttttactg gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca
    61741 atcggttttt cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa
    61801 gaaacatcaa gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg
    61861 aatatttatt ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat
    61921 caagcaaaaa cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga
    61981 aaaaagtgca ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc
    62041 aagcatttct aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa
    62101 cagcatattc accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt
    62161 atttttttca taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg
    62221 tggtggtagc ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc
    62281 aatattttgg ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt
    62341 tgtcattttt tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac
    62401 ttggatataa tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa
    62461 aaatgaaaaa gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag
    62521 aagtaaaaga attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg
    62581 atggttctgt acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt
    62641 cgatcatgaa tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc
    62701 gactaaagaa attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat
    62761 gatcggtgta aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt
    62821 attcaatcaa gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt
    62881 agctaaaaaa catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt
    62941 gtttcttcaa ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat
    63001 cataatgatt accactagtt cattggattt cgattcacta aagtccaaga taaccagtta
    63061 tttacgacaa accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc
    63121 atgtcattaa ggaatatgct gattttttta tgtttaatca tacaaaatag ttttttcata
    63181 aaaatatgga aaatatggat taattttgaa ataattgatt taaaaggaat acaaaagagt
    63241 gaatagtagg aaatagtggt ttagaactag tgggctgatt ttttatatat tttagtttac
    63301 ataatatata ttatacaaag tagatttaaa aaatgataaa cctctctcga acggtttttt
    63361 atcaaaaatc gtcatttttc catcaaaatc tgcttatgca aaattataaa aaaattttgc
    63421 agtttttctt actttttttg tcgtttaatt gaatgataaa aaccagtttc tttattcagt
    63481 taatcaaata tgaacgaatt agatatgaat cattgtattt gccgttgcgg aaggattttt
    63541 taattaacca cagataaaca ttgataacga ttcgcttatt gtaatcaaca gattaaattt
    63601 catatgttca tatgtacgga tacattttcg gaccgattaa ttttaaccat tctgtttatt
    63661 tgcttaccta ttagatatca ataaaaaact gcttaacagc tatttcagct gttaagcagt
    63721 tttaggattt ttagttcata ttcattgttt cttataatac agtgttcaat acttctctta
    63781 aagcacttgt tgatttgctg aacattgctt tttcttcttc gttgattgat aattcaacaa
    63841 cttcacgaac accattttcg tcaacgattg atggcacacc tgtaaagata tctttttcgt
    63901 cgtattcacc agttaaataa gcagatacag gcaatacagc ttgttcgtta ttcaagatag
    63961 cttttacgat acgtgcagta ctcataccaa taccatagta agttgctttt ttacgatcaa
    64021 tgatctcata agcagcatta cggactttat cagcaatcac atctaattca tcttgggcaa
    64081 tacgatggtc tttttctaca atttcaaaca ctggtttacc accgacagtt gtatgtgacc
    64141 atgcagcaac ttctgaatca ccatgttcac ctaagatata gccgtggacg ctgcgtgggt
    64201 caacttttaa tttcaatgcg atttctttac ggaaacgagt tgtatccaat gttgttccag
    64261 taccgataac acgtgatgtt ggcagaccag attcgttcca agcgatatat gtcaaaatat
    64321 ctactgggtt tgaagcaaca acgataatac cgtcgaatcc tgatcccatg atttctttta
    64381 cgatttggcg catgattgac gcatttgttt taactaaatc taaacgagat tgaccaggtt
    64441 tttggttgat accagctgtg ataacaacga tatcagcatc tttacattct tcatagccac
    64501 ctgaccatac agccacattt tcatcgcccc aagccatacc atctaaaagg tccaatgctt
    64561 ccccttctgc tttttcttga ttcacatcga tcaatactaa ttcattagag attccttggt
    64621 tgatcattgc ataagcgata cttgttccaa caaaacctgt accgacgatt actactttac
    64681 gacttgtttt tttcatgaat aaacagctcc ttttcataac tatttgttac tttcatgaaa
    64741 agtatagtct tgtttattca ttttatcaac atgttaatta taatagttgt tattataatt
    64801 tttgatggaa acgaattcct gaatacgcat aacagataaa tgacaaatgt ttacacctat
    64861 atcccctgat aatacaacca aaagtttaga actgaaaata aatccaaaaa aattcgctgc
    64921 aacgatataa taagacagca atcgcaccta aaacaatttt ccttcatatg ctttagtgaa
    64981 agattttctt cttaatgttt atttctccat tcattgggag tacatgttga atacttcttg
    65041 aataatttga ataaagaagt cgtttgttta tatccgatac gattagcgat ttcttccaac
    65101 gaaaaactcg tttcttgaat cagttccaac gcaaagtcga attttttctt tagaaccagt
    65161 tcttgaaagg aagcatcaaa atacattttc aatttattac taagatagac cggattgtag
    65221 ccaaaatgag ctgctgtttc ttttaacgta caagaagtaa aatgcttatc gatataatct
    65281 aatactctaa gcatctcatt ttctgcgcat ccatctgctg atgaaaagct ttgttgttct
    65341 aataaatgag tcaattcgat cagtaatagt gatgtataga gatttaacga acggatctta
    65401 tgactgtatg tatcaaaata aatcaacagc atattttcaa tcaaatgcgt caaacgactt
    65461 gtttttccgc cgcgaaaatg aatgaattgt gtatgttccc ctttttcttt tgatgcattt
    65521 atcaaaaaag aagaaagtag attatttgtg cttgccaaat gattcaatat ctctgtagat
    65581 aaggtttctt cttttaataa gatgttgatt aaaatatcct cttgccctgt gtattcgatt
    65641 ctttgagtag cttctttatc aatgatcacc acgtcacttt cttccatctc tatcctttct
    65701 ccattaataa tttgtaaaca tgtaccgcta tacatataat tgatttcaac aaaatcatgt
    65761 gtatgctcgg gaacttccgc atatcgacgg tgtttactga tatgaatatt tccctgttga
    65821 aatagatccc ctcggtggat ttttggcggt gtctgaagat ggtaagctaa gtttaaatca
    65881 ttgatattca ttccttgttc acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa
    65941 aatgtctaat tcagctagtt ccaattgact ccctcctttt tgtaagcggt ttctttcgct
    66001 tattagttca cgctgtactg ctattatatg caaagctatt tttggtttca taaagacttt
    66061 ctatggttgg gacatgaact tcctgtgatt ccgctattga gagaataacc acttcatact
    66121 atatttttga taagcgctta caaaaatgaa aggaacatgc ttatgaataa aaagatgatg
    66181 aaattttctc tgctgtcagt ctctcttatt ttgacttctg ctggtgcagt cacgtctgca
    66241 attcccagta tgtcagctac ttatgccacg cgttctttat ctgctgttga attattaacg
    66301 actgctccct cttttatggt cactattttt gttttgatta gtagttttat tgctaggaaa
    66361 atcggacaaa aacaaactgt gatcatcgga ttagtgattg catcgatttg tggtatcctc
    66421 cccctcttct cacaaagtta cagtattgtc ttagcttcaa gagctggact aggtattggc
    66481 tttggattga tcaattcttt ggcagtcagc atgattgctg ccttttatca cggagatgaa
    66541 cgagcctcta tgattggttt tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca
    66601 tttgttgcag gacagctagt acagttcggt tggcaatata cctttttagt ctacgccatc
    66661 agcttaccta ttcttgtctt gtttgttttg tttgttccta cacctgaaac aactgaactt
    66721 caatcgcatg gcacagcccc aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt
    66781 tatggtattt ttctcttgct ggtcatcgtg gtatataatg ctgtatcgat taaattagcc
    66841 acagtcctga cgacttattc tctaggaaac gaaactaatg cagcgaatat cttaacaatc
    66901 atgcaattgg caagtatggc tgccggagtt tgttttggct ggcttcagac aaaagcacat
    66961 cgctatatgc ttacactttc tttactactg atgggaatcg gctttgtatt gattgccact
    67021 gtgataaacc tgtatgtgat tgggtttggt gcgatcctaa caggaatttc tttttcgtta
    67081 ttcattcctt acttattcaa ccaagtaagt atccaagcgc ccaagtacgc ggagagtttc
    67141 aatacttcaa tcttattggt tggcgctaat ttaggtactt tcattgcacc atatggtttg
    67201 ctctttctct cttttgcttc tcttgggagt aaaacacttg ctgttttcgt caatggcagt
    67261 atcttgctgt tgattagcgc gcttattagc cttctagtag tacacaggac gaatagaaag
    67321 acagtgataa gagtagatga agaaataaaa gaacaattga caacaaaata aatttgatgg
    67381 aggtcgtgta aaatgacaaa tgaaaaacat aaagatagat ggctttggta tcctggagac
    67441 tttgagatcc gccatggact gctacaaaat ttccaacggg aagaacgagg ttttgactgg
    67501 ccagcgtact ggtacatgga tgattgccat cggaacgtca agtttaagag atattacttt
    67561 cttgatcaac cttccatgtt taaagtgacg atccaaggag ttgggtatgt agaaattaat
    67621 ggacaaaaac atccatgtgg aaaatggctg acttgtccag ctggtaaagc aaaaattcgt
    67681 atctttgtag gacatacatc agggctacca gcgatgttta tcgagggaga cgaagtaaaa
    67741 tcagatattg gctggacagc aagtaatttt attgaagaat atccagctgg ctggagtccg
    67801 ctttatgtcg atatcgcaaa agatccaaat caaatctatt atcaaaaaga atgcgtccat
    67861 ccagtcactg aaacggagat tggcggagga atcttattcg attttgggcg agcagtgaat
    67921 ggctgtgtag aactaatatc tctggatgac catatcgaat ctatcactct atgttatgga
    67981 gagtccgatg tagaagctat ggatatcgat tattgctact ataagcaaat aggaatgaca
    68041 agaaaaatga aagctcgtaa acgcgctttt cgctatttgt ttataccaga ggtttctact
    68101 ggaatggttc aacttcaagt ttttcatgaa tttttatctc tttcgaaaca agctgctttt
    68161 tcaagcgatt cttctttact gaatcagatc tggtcagtat cagaagaagc cttcaagtta
    68221 tgtagtgatt tgttttttat cgacggtatc aaacgagatc gctggatttg gtctggagat
    68281 gcgtatcaat cattcatgat caatcaatat cttttttttg acgaggaaat caataaacga
    68341 acgatattag cattaagagg acaaaatgaa atcaagcagc atatgaatac tatcgttgac
    68401 tattcgatct tgtggttgat tggtatcgaa aaccactata tgatgacgaa agactatgaa
    68461 tttttacgca tcgtctaccc taagatgcaa tcattaatga gatacttgat ggagcaaacg
    68521 aatgaattag ggtttattta tgggagagca aaagattgga tatttattga ttggtctgaa
    68581 atggataaag aaggaacgct tgctgcagaa caagtcttat tactgaaagc ctatcattcc
    68641 atgatcactt gcggagatat cttagctgaa acatatggag agaacgaaaa ttatcatact
    68701 catctctatc aagaaaaata tcaactattg aaacaaaacc tatttgacta cttttgggat
    68761 gaagaacttg gcgcttttat cgattgctat gaatctggga gaagaaatgt gacgcgtcat
    68821 gcgaatattt tcgctgtttt attcgatttt gctaacaaag accaacagca atcgatctta
    68881 aagaatgtct tattgaatga cactatcaca cagattacta ctccatattt caaattctat
    68941 gaacaagatg ctttatgcaa actcggacag acgaatatcg tctaccaaac gattcttgat
    69001 tattggggag ggatgttatc tcatgatgcg gtaactttct gggaagaata cgaccctact
    69061 caaacaggaa atgaaaagta cagtatgtat ggagatcctt atggaaaaag cttatgccat
    69121 gcatggggag caagcccaat ctatctttta ggacgctatt ttatcggttt gtatccaaca
    69181 aagccaggtt atgaagaatt tatgatacaa cctaatctag attcatttga aacactgaat
    69241 tgtacgctac ctatcaaaga tggaaagatt tcgcttgaaa aaacaaaaga taagatcgtg
    69301 attacttctg atagaacagg cggaacagtg aaaatgaatg gtaaaagtta tcctcttcta
    69361 gcaaaacagc cacttgtctt aacaagctat tcaaaaataa atgcataaaa caaaaaactg
    69421 cttgcgacat atcagaagac tttaaatctt cttctgatat tgtccaagca atttttattt
    69481 tgaagtttgc ttaattctgt tctttagaaa caatgtttgt tctttttccg gcaaaaagaa
    69541 agctagataa tacgacaagt aaaaagaaag cgatgattcc aactgtcaag gctatttgac
    69601 cgtattctcc tagtttccca agtacaagtc ctggtactag ataatcagta tcggcaaatg
    69661 tagaagaagc gattttaaga tctcctaata ctggaaggat aaataatggc agccaactaa
    69721 tcaataagct attgaaagca gaacctaata ccgcccctct tctaccgcca gtcgagttgc
    69781 cgaagacacc agaagcagct ccgcagaaga aatgtcctac taccccagga atgataactg
    69841 tagtttgcaa gcctatcata gcaaacatgg atagtgttcc tacaaagaag ctaacaaaaa
    69901 agccaattaa tacagcatta ggtgcatatg gaaaaacgat aggaacatct aatgctggtt
    69961 tagaatcggg tacgagtttt ttagcaatcc cttggaatgc tggaacgatt tctgccaaca
    70021 ccattcgaac cccttgaagg acgataacaa acccagctgt gaatgttcca gcttggatca
    70081 gtgcaaagat tacagcgttg tctccattgc ttagttctct ttcaacaaat ccaggaccag
    70141 ctaacaaagc aagtgcgaca tatacgatcg acatcaagag catgatcgat acagtagaat
    70201 ctcgcaaaaa gctgaaactt ttaggaatct ttatgtcctc tgtagaacgg tctttgtttc
    70261 caaaaaactt tccgatccat ccactagaag cataaccgat atttccggta tgccctaacg
    70321 caacttgatt gtttccagtg atgttcttca taaacggttg acaaagagca ggcgtaatag
    70381 ttaatagtgt tccttcgaag accccgccta ataagatcat ccacaaattt gcagtaaacc
    70441 ccgcgcttac taaaatgact gctgttagac aagaaacata aagcattgct tgacctgtaa
    70501 gaaaaatgaa tttatatctt gtgaatctag caagtaaaat attcacgatc atccctacta
    70561 gcatgatcaa agcagtaggt gtaccgtatt gttgcaaagc aatcgccact actgcttcat
    70621 tgctaggaac tactccttga gtatgcagag ctgtttggaa catcgaagca aaaggggtta
    70681 gtgaattact gattaaacca gcaccgccga tcagtactag gaatccgaca aatgttttga
    70741 tagttccttg gatcacttct gaaaatttct tcttctgaag aacgagacca agcagtgcaa
    70801 tcaaagaaac gaggatggag ggctgactga cgattgacac aaacaaattc aacaatttat
    70861 ccattgatag ttcctccctt gaatcatttt tattcttttg acaagatctt cgttactttt
    70921 tcttttactt cttcgctgtc aatgatcgat tgcaaaggta cgagcttttc atcaggtata
    70981 gaagttagcg tatctttcaa agaatgctca atgaagaaat aatcagccat atcgctagat
    71041 acacttccaa catccgtatg acttacttca aactcgtcca catctgctcc aagatctgca
    71101 agtgcttgtt tgatgttcat ctctaccata aaacttgttc ctaatccact ttgacatact
    71161 gcaaaaattt tcatatcatt cgtctccttt tcttatgatc gctaatattt cttcttttgt
    71221 tgatgcgtgt aataaatctt gaagattttg tttattagaa agtagctttg ttaaatttgc
    71281 caaagcccga agatgtgcct cattatcgat agcagctaaa cagatgaaca aactaatggg
    71341 atgggctgga ttatcaagca gatccacagg ttgttgaagt ttcagcaaag acatccctac
    71401 ttcgtttacg ccatcttctg gacgagcatg aggtaatgcg atgtgatcgc caatatggat
    71461 aaacgcacca tactgctcca cgcgctcgat catagctttg atataacttt cttctatttt
    71521 attttctgat aatagcggct gtgcggcaaa tgtgattgct tcttgccagt tttttggttg
    71581 atccgataat tggatcattt cttctgttaa taattcagat agcataggac gttgatcctc
    71641 cttcttttct aaaagtttgt tcattttacg atttaatact tgatagagct tttcttcgtc
    71701 tacgccttct tttaattcga tataaggcag tagcgccgac aacaattctt gggcagaagg
    71761 cattgaaaat cctggtatca gccaatctct ttgtacttga ttgatcagct gatttttttc
    71821 caagtcagtc atgattggat gcagaacata gagagacttt ttcttatcag taagtgtgag
    71881 aggtacagta gaaaaaataa tatcgtaaga agtttcgtca acctcgtgta agcggtcagc
    71941 tgaattagtc tctgtaaaaa agatcatcgg gaataattgc tgaagttcac tttttaagat
    72001 caatgaagag ctaatgccac ttggacagac gatcacggct cttatttttc tatttctttc
    72061 ttctgcgtcc gcttttctga tctctccgcc aaatagtata gtgaaaaaac caatctcttc
    72121 ttcagggatg ctcttcccta ttcgtctttc taacggaagt aatgcttttc ttgtcatttc
    72181 gtagatggaa gcatattgat gcttaatttg atcgatcatc acattaggta aataaaaaga
    72241 ataggaaata cgaaaatagg cagggaccaa gtgataaaag acattcatca ataattcacg
    72301 aaacgaagca aattctacgg ccatcaggaa ttccatgcga tggatgactt ctgaagaaca
    72361 ttccagcaaa aaatcaagtg caggctcctg gacatctcct tcaacgatcg atgctaagca
    72421 aagagagaaa aatataatct ccgaattttg atgttcggaa tagccaaggc cagtcaatac
    72481 ttgtcgacta atgtcagtga ctgcaagaga agccaacagt tcagcatctt ttaccagttc
    72541 catggatgtt tttccaattc gaatggttgt caaactcaaa taataagcta attcatccat
    72601 tcgatttggt gcgaatcgaa gatctgatag tgaaatcgtt ttatctagca aatctctaac
    72661 tttggcgtaa taatcaatgg aaagggttgc ggttaaatac gtgagcccaa atcttccagc
    72721 aagtgactgc atcaaacgag cagtgcgatt ttttgcttct cttcttaata ttgcttctga
    72781 ccctagcagc ctaaaaccag ttttcctagt atacgacagc tgacaatctt ccacttctaa
    72841 ttcatttctt atctttttga tatctgacag gactgtccct ttagaaacct gaagaaaatc
    72901 ttgaaaatga aaaacagaaa cagacgttgc agtcgaataa accatcaaat aaagaagatc
    72961 tcttcgctct tgttcggtat acacagcttc aaatcgagta atatccaaaa atttttctgc
    73021 ccagtcgatt gctcggaatt tctttatacg gatacgggta tcatccacat attcggcaaa
    73081 aggagttaat tgtttgatcg cctcgcttgt atttacttcg atgatctctg ctgctttttc
    73141 tagactgatt tctcttggtg ttttcaggag ataactaatt ttgtagttca cagcgctgtt
    73201 cacctcctac ttatattcta gtatggtagc gcttttaaat cgaggggtgt ttggtacaac
    73261 ttacagttgg atttttttta cgactgtacc gaacgatttc tatcgaatgg atacagaaaa
    73321 aaagcaggtg accaaatgtc tcctgctttt atgaattgat ttcgtccgat caatgatgat
    73381 catgtttatt ttttgaggtc agttcttcaa atttttcttt gacatcttca accgcttttt
    73441 cgatatcttt ttcaaagtga ttgggctcat taggctttgt ttcctcttct acagaatttg
    73501 aaccagataa agctgtttct ttattttctg tttcaccaga gtttgtgcca gctaatgatt
    73561 cttctttgtc ttctgtttca ccagaattag ttcctgctaa agccacttct tcttctgttt
    73621 taggggccat tgaacctctt aatcgtttgt gtttttcacc gtcataattt gtcatgttgg
    73681 ttcctcctag tagttctata ctttttcatc gtacaataag acagtttatg cgtcaaaaaa
    73741 caaacccgat tcttacaaat atgtcatatt tttccttctg attcttgaat gcttttgatt
    73801 ccttcttcca aaattttcat ttgtaagatt gtttcttcat ctttgatgac tttttctgcg
    73861 ccattaacta atgtctcgta aatcccttcg tagacacggc tgtaatcacc aacctcggaa
    73921 attacttttt cttcatgata actcccttgt tcatctatat aggtcaatac accataatgt
    73981 tcgggcaaat cgataccaaa gtcttcatga tcaggcatgt aaaacatttt cagatgttct
    74041 tcttgtcgat cttttgtttg tttcacgaac atcccttttt ttccgtaaac gacgaaactt
    74101 ggacgttctt tgatgcggaa ataactggat tttatcgaaa ttttcatatt tccatagaaa
    74161 aaatccagat cgaagtaatc attcattcgt cctttcccta aaagttgacg tacatcatag
    74221 ctgatatcat ctggttcgcc gaaataagaa agaacttgat ctactgtatg acaagcatgt
    74281 ccatataaat agctgttcgc agtagaaaat tcatgcgtat tttctggtac ttccggtctg
    74341 aaataatcat aatgcatttc tacttctaaa atatccccta attttccgct ttcgattact
    74401 ttttggactg tcaagaagtc agaatcgaac cgtctgtttt ggtaacattg gataaataga
    74461 cctttttctt ttgctaatgc aaataactct tctgcttcct ttgacgtttc tgcaaaaggt
    74521 ttctctacta gtacattctt accatgttcg agtgccattt ttccatattc ataatgaaat
    74581 tgactaggtg tagtgatgac aactaattgg atctcaggat catcccaaag atcagacagc
    74641 tgatcagtat attcgatgtc ttcgaatgtt ttccacactg gttcagatgg ggaaaaaatt
    74701 tttttgatat tgattttgtt cgtaagtttt cttgaaaaag gcaaatgata gcgatttgta
    74761 ctctttccat tgccaagata agcgattgta agcatgagtg gtgcctcctt ttattgatgt
    74821 tttcagtata tcctagaaaa gcgcagaaag tatttataat agctattctg gtaaaaaagt
    74881 tcagaaaaac acttgttatc attcggtttg gtgaaattaa tgttcaaagt aaggataaaa
    74941 atcaaaaaga tagtagcgga accaagtgtt tatcagtaag atagaatttg gaggtcagat
    75001 catgcttatt gaagaaaaac taacaaaaca agagttgttt accacgacag agaaacggat
    75061 tgtcgattac atccgtagga atatcgaagc tgcagtctac atgacgattg aagaattagc
    75121 aaaagctacg tatacttccc attcagctat cattcgttta tgcaaaaaaa tgggattcag
    75181 cggatttaaa gaattccgtt tcgagctggg gcgggaagtt catcaattga ttgctcagtt
    75241 caatcaaacg attgatccta attttccttt ttcaagtgaa gatagcactc agcaaattgc
    75301 aaagaaaatg gcggatttat cgatccagtc aataaaaaaa gcacaattgc agattgaaca
    75361 tgaagattta gatcaaatcg gtaaagtatt gaacaaggca cagcgaatct ttctttttgc
    75421 aaaaggtgat tctcaaatca cggcaaggaa attccagaat aaaatggtca agttgaataa
    75481 gtttttgatc atggctgaag aatacagtga ttcttcttgg aatgcagcca atttgactag
    75541 cgaggattgc gccattttta ttagctacag cggtcgcatc catcattatg aacgaataat
    75601 gacctatttg aagcatgtag aggcccctac cttgctgatt accggaaatc agcattcaga
    75661 aatggccaaa caggctgata tgtgtcttgt tatatcgcag gaggaatatg attttgctaa
    75721 agtagcgact ttctcctctc aaattgcctt cgattacgta ttaaatacgt tattctcagt
    75781 gatatattct caaaattttg aagagaatat ccgcaacttg aaaaacaaac aagaattgat
    75841 gcaaaaaggc cttcttaaag aatatcggaa aacttaagaa aagatcctca tttagtaata
    75901 agaaagatag tgtttgatag gctctctttg atatcatcat aaaaacaggc tgaagttttc
    75961 tttttcaaac ctcagcctgt ttgtttacgg aacgattcga atagatatat tatagaaatg
    76021 aggaatagct aacttctgtt tcttattttg aacgattgac gaaaaagata tataagaagg
    76081 aggaaaatca gtatacatgc agctaacaat ccatacagga aaaatcgttt gttctcggat
    76141 actttctgta cttctttttg aacttctttt gcatgataag gaacccggtg ccctctgaca
    76201 agtaagcgat gactgttgac catataaggt gtacaagtta ggagtgtcac gaaatcctgc
    76261 cctttttcga ttctcagatc ttttgtatca gtcggttcta ccgtcttgat ctgatcgact
    76321 tgataagcga gtgtccgtcc attgatttcg atatagaaag aatcacctaa agtcagtttc
    76381 ggtaagtctg tgaacaactt tgcttgaggc agtcctcgat ggctggaaat aacggcatgt
    76441 gtattttctc cccccgtagg ataagacgtt ccttccaata atgaggcacc tttttctagc
    76501 agcacagtat tcgtttcatc aaaaattggc aaatggacat tgatctcagg gatcgtcaaa
    76561 ataccgatcg tatgtttctc aaaataggtt ctatcctttt tcgcaggttc ttttttctta
    76621 gtaaagggat ctgcacctgg atttccacct tcttttgcta attgctggtt tttctttgtc
    76681 atgttttctt ggattttagc catgacttct tcattttctt tgaccgcttg ttgctgataa
    76741 tgagagatga tctgctgatc cagataatta ttcagcgcgt cgctaacaaa tggataagct
    76801 agtatcccta ttcctgagag aagcaagaaa agcatcagca gatctagaag tttccttttc
    76861 tttggtttca tttgtgttct ccttgtgatg actgtttgat aataaaagaa aaaggagcat
    76921 ttgcgtaatc cgctgctcct ttttccaatt cttgcaaaaa gactaaatct gactgtgctt
    76981 gcgtctagca aagtacagtc cagcaatcag tagaaggact actcctgcac cgatatacac
    77041 atagattccc ttaccgcctg ttgaaggaag tgtacctttg tgtttatttg gtattttttc
    77101 aggagaaatc aactgtcctg ctgttacata agattgttca tcgatagtaa atgctacacg
    77161 gtttgttaat ggaacatatt tttctggcgc tttcgtttct tccagatagt acgtgccata
    77221 ttttagacct gtcacatcga ttaaaccgtt acttgtggtt gtaaaaaccg ttgctgattc
    77281 tttcgccgat acccagctga cggcttttgt ggatgggtcg atcgataaat atttcgctgt
    77341 gtcactatct tgatcacgaa cgacgaattc tgctccagca agtgtttggt ctgatgtaac
    77401 gtcaccatct actttaacaa atcgtttgcc cccagtaacg acatcgactg acggtggtgt
    77461 ttgatcattt gtatgcccgt tatcgacatt tgcttggtta gaaaatcctt tggttggatc
    77521 tgctttttcg ttcaaatgca tatagtaaac gaatttcaat gtaccgccag gagttaatga
    77581 aggaatataa ttcggatcaa ctgaaaccgt gaatccgtct gtttgctcag tgacagaata
    77641 atttactggg tcgatttctt tatttccatc atataaggca taagcatacg ttcctgaaga
    77701 atcattatca aatgttaaag cagcgtcatg agtatcgata agtttgaatg ttgtgtactt
    77761 gttttgcgtt ccttctttat cagcgatacc taatgggata ttgacagaaa tctcatattg
    77821 aattttttca ccgattgcga catcttttcc attcaattga ggtgttgttt tatcaacttt
    77881 agacgtatca tttttgatgg tcttttctac tggtgtttgg ctatttgcca ggatctcaaa
    77941 aggcgtgatt gtttgctttt caatcatttc cgcattatca ggagctttta cttcttctaa
    78001 attatatttt ccaacttcta aatgattaac gatcaactgg cctttttcaa tagatgcctc
    78061 ggcaaagtca ttgttgccga tgtcataaga atgaccagta atgaaatgtt tggctttggt
    78121 ttgatcagtt gtccaagtgt acaatccatc tgtgacactt tggatgtatt ttttgacgct
    78181 tggtgttcct tcttctttag aaataataaa ttctgctcca tttagtgctt cgttttcggc
    78241 agtaccgatt tttgtaactt tcaacgttcc atcattaccg actgtatttt tagggtagag
    78301 atggatagta tctagttctt ctgtcccgta tttataagag ccatctgctt gtttgatcat
    78361 ctcatataca gggaaagcta aaaccatgtt tgcggcagct gacactccgt cttttggttc
    78421 ttctttgatc gtgtagactg catctttccc attttgtttt ttaggtaaag ataaagtgac
    78481 atttccatca gcatctgtcg ttcctgaagc aactggtgta ccaggagtca aagactggac
    78541 tgcttgtttt gctgcatcca cggacgctcc tttatctcgt tgcgcataaa attcttgagt
    78601 gacgttataa actgaaaatg aaatatcggc taatccttgg tattgatcga attcgctcat
    78661 ttctttcccg ctgttttgga ttaaaggatc gggtaaatca gtcattttct ttttatgaag
    78721 gatgacttga gctgctgtct cctctgcaaa cacttttttt gcgccgaagc tgtttgtgag
    78781 taatggtaaa ataaggaaaa ggactcctaa cataacgttt atttttttat gatttttcat
    78841 tttttcactc ctgttctttt tttgataaac aaccagattg caaagcttgc gaataccata
    78901 ccaacaacgc ctaaaagtgt atttttcgtt tcgtttgttt ttggaagaaa tggacggcta
    78961 ttttcttttt tattgactac tttcaatata ctgtccgcct ctttatcctc agaaagaaaa
    79021 gttgaaatgg taaacggaat ttctgtttca cttaaaatat aaccagaagg tgcttcgatc
    79081 tcttttaatc gataacttcc tgttttcaac ccggaaattg aaaaagcgcc atttttatcg
    79141 gaaatcaggt gaagcgcatc tgattcgttc tttgtccact tgtatccgtt ggctgtttgc
    79201 tggaggtaat ttcctgcttt gtttttaata aggaatactg cgccagctaa tcgtttatct
    79261 ggtttatttg tctcgatttt gacaaattgc ttactccctg tatatacaga gactttcttt
    79321 gtactgacac caaaaccaaa atccagtgtt ccgttgttgt tgatttcctt gttcgcctgt
    79381 gctgtactgc ttagctgcat ctgataagaa atggtcagtt tttttccagc atatttttcc
    79441 aacgaaggga tcgaaaataa aacatcaaat ccgtgaggtt tcttgtgtat gacaaagcct
    79501 tctgtcagct tttctccatt cgatgaaatc gttaaactat taggtaaaaa cgttaatgct
    79561 tcatccgcac tatctgacaa ttcgaacttt tgatagtcaa ggatatttac aggaattgtc
    79621 gtatgcaagg aataagtgat tttttcacca atcgtaaaat catttctagg ctcttctaat
    79681 accttctcaa aaggtggatc agggtagtcg ttttcttcat ttttaggata caaatggata
    79741 gttgaaagtt tttgattgtt ttgtccatgt acaggaagaa caactaccat attctctgcc
    79801 ttttctttga cgacttctgg tactttggat tcaatgaaaa gatacgcttt atctcttttt
    79861 tcttgatctt tagcggccag agaaaaagaa gcgataccat cttcgttgtt aagagttgta
    79921 gttgttgctt ctgcggtaaa cataccggaa gacgcaccgt tttttgcgat ctctgcttgt
    79981 gcttcttcta ccgttttgcc cttttcccgt agatggtaaa aagaatctgt gacatcataa
    80041 acttggaatg tgacaccatt taatcctcga tacgtttgta ataaagcttt ttcttgtccg
    80101 tcatttggat gatttttcgg cagttgcccg tttgggaaaa gcaatttgtg taaagtaatc
    80161 tgaaccattt ctgtttctgt ttctgttgct acctgagtaa aagctggttt aaatagtaac
    80221 aagaagagac acatactaag ccaaccaagt tttttcatat ttagctgccc cctctttctt
    80281 ttttcaaaaa aagataccaa atagaaaacg cacaaccaat catccctact agatagattc
    80341 ctaaacggcc aattcctccc gtttcaggta atggtacttt tgcctgattc gtgatgtcta
    80401 aagaaatctg gttgtttttg gctcctggtg acagaacaga ttcatgatct tgtccatcca
    80461 cttgaattga cccatcttcg tgtataacta tagtaactgg ctcttttaga ccttggtatc
    80521 cttctggtgt aaaagtttcg gtcagcgtat aagttccagg ttttaaattt tcgaatagaa
    80581 aggtatctgt ttcttgtcca tcttttggcg attctaagtc catttctggt ccctgcagtc
    80641 tgaattttgc tccttttaat gtctgtccgt tgtcttcttt tttgtgtacc gttagatcaa
    80701 acgcttttag tcgattttga tgagtgagcg tccatacatt atttgtcatc tcggtatttt
    80761 ttcccgtgta aatgattttt ccatatttat cgatttggaa ttcataattt cccggcgaag
    80821 tatcatatcc ggcaggacca gctgtttcaa cgactcgata tttaccaggt gtgagtgaat
    80881 caaaactggc aagacctttt tcattcgttg tttggctgtc aattggctga taagtaccat
    80941 ttgcttcttt tttctgtagg gaaaatgctg ctcctttaag atttatctct ttgtccgtcc
    81001 cttgcatcgc atacttacgt actgctacag gaacttcaac aaaaggattt tcaatagtca
    81061 actggatcgt atcatcggaa gtagtgactg tttttccatc aatggttacc gtaccatcag
    81121 aagcgatctt gatttcccaa gtagtctttt tgcttaatcc atgcccttct ggagcttttg
    81181 tttctgtcag cgtataggtc atttcttttt gcaatttgac attttctgga agagaatagg
    81241 ttccgtcgcc atgatctgtt agtaaagtat caatagaatc tcctgttaat ttgaaaacag
    81301 cgccaataag atccttttca cctgtagagg aatttttcgt tattttcaag tttaacggtg
    81361 tgaattgttt agtatttttc catgtcattg catctatttg ttgagaatcg tatcctggta
    81421 caggtaattc tttgattgtt tggtaactga atgcttgacc ttgattattg tattgaggta
    81481 gtgataatat ctcttgataa ctttctccgc tatttgcaga taatttgtca atgtctgcac
    81541 gttcccacgt atttgttgta tctttagctg gtttaatgat tcgaatatat ccgtttttcc
    81601 aagctgcagc atttgtcgta tgttcccgtt gaatctcaaa agtaacttga tctggacgat
    81661 cagctagatt gttgtcaaat tcttcccata actttttgat gtgaagactg actccgggag
    81721 ctttagcaga tggtataccg aattcagcta attcattggt atcaatgttt ggctggaaag
    81781 ttgtcctgcc gttcatttga taccagaaat ttggatggaa gtcctcattt tctgtttgga
    81841 ttctcacttg gtaatggatt tggatttctt ggtcttttcc taaatagatc tgattgctct
    81901 tgatggtatt tccttctatg gaaatagatg gagtgaccgt tgtaggactt gtccccacac
    81961 tcttgactga aagtgtacca ggctgataaa tgaatggttc tgcgattgga tcatttattt
    82021 gtccattgct tacagttgct gagatctgta cagctttttt ggctaggtat tcagagatat
    82081 ctgttgcatg atcagctgat tcatagtaaa gatcgccttt ttcatctgat gaaaccattt
    82141 gtcgcatacg agactctact tctgcttttg agagaccagc tgccggatcg ctttgaagtt
    82201 ggatgccaag accatgtatt tcgattcctc gttctttgag tgccatcgct tctccgatgg
    82261 ttgcgataaa cgtactgtcg attcttctgg atagattgtt ttggtcaggt gcatcataga
    82321 ttcttgagat tagagaagta tttcccggcc gatcttgcgt attagaaaac tgagttccgt
    82381 aataattgct gcttgattgt gcgtgtaccc gctgtacttt ataggaaaat gttggtacac
    82441 catccgtcag caaaacgatc acttttttat gtccatttgg aacggatagc atgcttcctg
    82501 catctcttag tgctttttgt gtaaaagtac caccatttgt ccgtgaaggt gtaatggatt
    82561 ttacttgatt tttcactgaa tcaaatgaac ccatctgtac tgcaccgtta ctgtagctat
    82621 atccttcgct tgagtagccg acatatccca tattgatttt gtctgtgata ccgctatctg
    82681 ctaaagtatc gacaaaacga tcgacaccaa tctttacttc accgatccga ttattgtcgt
    82741 tcatacttcc tgaccagtct acgaccaata cgagatcaag aggcgtgata tctttttgta
    82801 catttccacg agcattcaaa taaacatcaa acaatccggg tgtacttgtt tcctttgcat
    82861 actttcgtat cgcatagtct gcttgattac cggttcctcc atattcgata tacgaatggg
    82921 tccgatcatt aggatctcca ttccaagaat ttataccatc ccattgattt gttccgtttt
    82981 tattgccttg atgatcaaga acatttgtat ttcctgtagg ttgccaactg gctttaggat
    83041 aagtgccctg atcatctgtt gtatattctg taggaatcgt ttgattagat ggcacactca
    83101 cagctggcaa ggataatgta ggcaatgtaa atgggctgac agaattttcg ttttcttttg
    83161 atgattcttc ttctatttcg tctggctttg gtactgacac atctgtatct ttcttatttt
    83221 ctgtctcatc aataatgtca tattctttat tatcgacgat cagcttgttt gcctggctgt
    83281 ggtaaagtgc tggatcgatt ggaaaactca gatcgatagt ttgtttagtg tttgatgaga
    83341 tttccaacgt aattttcccg ctatttacct gataagcata gccatctcct tcttttcttt
    83401 ccacatcact taagtaattt tcgactagac caatttcttt ttctatcttt tcagcactgg
    83461 tgttattcgc atttagcttg atgttcagtg ctttttttcc agactgatcc gaaatgactt
    83521 cagctgaaat tgtttctaaa actgttggat catctgtagt gtctgctatt gcagataatg
    83581 gcacaaagtt tgataaactc aatattatca gcatgaaaat aactttcagt ttcttccctg
    83641 tggtcgtcat ttttcccctc ctttttcttt ttttctgttt tgacgtgaat agtgaaaatt
    83701 attaaaataa acaggcgtta cataatttta tttattttaa tatgataatt attatacgaa
    83761 gggcgctaaa gggaatcaca ccaattttca taccaattgg tttcatttat gaagttgatt
    83821 ccttcgatta atgagcgttt cattgaaaga aaagggttga gggagaaaaa catatgatca
    83881 ggttgttgga acgaaactat agaaagatgg aaaaaatcac tcgaattctg tccatttctg
    83941 acaagtggat gactaaaaaa gaacttgcca aacgaattgg tagttcagaa tctacatttg
    84001 taaggtatat cgaagagatc aaaatccgct ggggaacgga tgtaacgatt caatcttcac
    84061 aaaaattggg gtatcgtctt gaacatttga atgtaccagc ttatttaaat gttctgatgg
    84121 aaatgtcgca aagttctaca aacagtcgtt tattaaatga gattattgtc aatccgggta
    84181 aaacgattga gtattattgt gaaaaaattt ccatcagccg gtcttctttt acacgtaagt
    84241 tgaaacaatg caatcaaatt ttagaaagct atttattaaa agtgatcgtt gaccaaggtt
    84301 ttcagttaac tagccttaaa agtgagctcc agttgcgtat ctttgttacc tttttctttt
    84361 taagttacta cggaagacgc acgttgccat ttccaatcga aaaaaagaag atcaaagaaa
    84421 ttatggtcaa gaataattgt cagatgaatt tgtttcagga agaaagccca tatgagcaat
    84481 ctttctttat tatgtatttt gttgtgcatc tcatgagaga atcccaagga actccatttg
    84541 aatggaaagc tggagactct tatgtacagt tgataaatat cgataaaaaa gacttcttaa
    84601 tcttgaaaaa tctcttagga aaattgaagt acagcagcta tataaaagta gtgaactatt
    84661 ttgtcaatac gattttttca gaattttcgg agaaagaaaa aaatgaaatc agtgagcaga
    84721 tacgaaaaaa tatgtcctac tgttcgtttt catccgctga ttgcaaagaa aaaaacgaag
    84781 aatatgttgt tcaattatta acgcaaattt attttttctc ccgtattttc ccatacgata
    84841 cgactcattt gacgatccgt gcaacttact ttgcacagca gatgcaacac cgccagatac
    84901 gtctatatgc tggatttcgt gcagaacttc aattttattc cactctactg aatcagaatc
    84961 tgatttatta tttatctagc tatatctttt ggatgcttat cggtaatcct gaaattcacc
    85021 attatacttc tgataaaaaa atactgatta tcagcgactt aagtcttcga catagtcaat
    85081 atattgaaga atatatcagt gatattcttg ctgtgcataa aattcactct gagacaactg
    85141 ctattactga agatcagctg tcgaaataca atcttctcga gtatgatctg atcgttacga
    85201 atcaacccat tttgaatgcc catgttcctc atatattgat tgatgatagt gtgtcattcg
    85261 caaatgaaga agaactcatc cgcttattcg agttatgaat aatcaaaaaa cagctatgta
    85321 taaaccaccc tgataaaaat ttcaggacaa atacacatag ctgtttcttt ttattgtatt
    85381 ccttcagcaa tccaacgaga tttatagcga aagtctttta acaactcttc ttttgtccaa
    85441 ttatttgcaa tgagatccgt caaagtgttc attacgaggg tcatggaaaa gtataggaaa
    85501 ctaactgctt cttccgacga ttgaatggag aaatgattga ttcttaataa ttccagccat
    85561 ttttcgttca ttggagaatc agccgatagt tctctacgtt tggttgtctg ctgatcattt
    85621 cgctgggtta acaagtataa cctttgccaa tagatacttt cgtgatctaa gtcgcagcac
    85681 cactctaaat atttttcgat ccccaaaaag aaatgttgtt tgttttcgct aatgaatttt
    85741 aagatatctt gatggactat gtgagaactg cttttgatcg tatacaaata agcatcttcc
    85801 aaatcttgaa aatatttata aaaagcaccg cgcgacatcc ccatcttgtc aacgatttct
    85861 gagactttga cttggctgat gtgctgtgag caaaatatct ccagtaatag gtcatcgatt
    85921 ctctgtttct ttgcgttcga taaattgaaa aaagtttctt ttggcatcca catctctcct
    85981 ttatctctga ttatacgtgg atagcccttc attgtcatga aggattcatt ccttcttttt
    86041 ctcttcttct gtcaagtacc catcttccat tcgataaacg cgatcactcc attgaatcat
    86101 acggacatca tgagtaacca taatagtcgc tttttgtttc tcatgagctt ctttaactag
    86161 taatttgacg acttcatatg catgatctgt atccaaacta gctgttggtt cgtctgctaa
    86221 gatcaagctt ggctcattat ataaagcgcg agcaattgcc actctctgtc tttctccacc
    86281 agaaaggtct ttaggataac tatttttaag tgactggata tctaaagatt gcagcaagga
    86341 atcgattttt gttgaatctt ctttttttga agtaacttgg tcaactagtt caaattgttc
    86401 ctttaccgtt aaaaaaggaa tcaaattaga gctttggaga ataaatccta tttctttaaa
    86461 gcgtaaatct gctcgttttt tttctggaag ctcagttagt gagtgaccgt tgatcactat
    86521 ttctccggaa gtcggtgttt gcaatcctcc tgaaatggta aggaaagtac ttttacctga
    86581 accagaagga ccaatgatgc tgacgaattc tccttccttt acataaaaat caattgcttt
    86641 caaagctgag acctttgtat gaccagaacc aaatgttttg gtaacttttt tcatcgttaa
    86701 tatttttttc atttttatta acctcctatt gctgtaattg ggtctacttt ggttatcgta
    86761 cgtatcgaaa acaatccgcc tagcattgcg acaatgatca aaatcccact atatagcaac
    86821 cattgtgacc aaaaaacagc aaaaggcatc gcgcttggta aaatcagact agtcaaataa
    86881 gcaaacaaaa gagcaagcag aacgcctacc accccaacaa taaaggactg tgctactaaa
    86941 gatttagcga tgaagaaatt tttgatccct tgtgctttca ttactccaaa aatagcagtc
    87001 ttctgaagtg tcatcacgta catgaaaata ccgaccacgg cagcaaccac taagaacaaa
    87061 aagtatatca tcgcatttaa ggtcatattt tgggcagaat agccaggaat actttcaatg
    87121 aatgtcccaa tagttaataa ctgtagaccc ttcgatgctt tttctttcga aattttagct
    87181 gaatccttta ttacgatccc attgatcggc tgttcttctt cagaagcaaa cggctgttgt
    87241 ccatacttca aagcagtcca cgtatctagg gacgcataga tgacgggaga aactgtgtaa
    87301 taagtttcag gaaagatacc gacaatcgtc aaaggatcgt cgtaactgcc aattttgatc
    87361 gtatctccaa ttttatagcc tgaatcagcc agattttgag agatgatgat ttcattcttt
    87421 gttttaaatt catgcccttt cgtgagtttt ggtaataaaa atgcttcttt cgttgtgccg
    87481 aaaactgtaa cattttcttt gttttttcct ttgattgctc cgctgtacaa tccgattggt
    87541 gctttctctc ctccttcaat gtatttcaga tctcctcttg ttaattgtga agctgcaaat
    87601 acttgatttg catcctccga caaaacaatc tcttgtgcgt cccaatcatc gattgctttt
    87661 ttaaactctt ctgaaagtcc attcgctagt cctgacaaca tgaatacgac atacgcaatc
    87721 aagaacataa taccgacgat caagccatat cgtaatttag aatagcgaat ttctttgata
    87781 gctagaaaca tttgtttttc ctccctatga tagattgata gtgacaaaat gtcacttgtt
    87841 gaatagtgta actctaaagt gacaatttgt cacttgtgtt gtttttttct tatcaataaa
    87901 ttgtctaaga catctttcga taaaacctta acatgagtca taaaaaattt atttaagcct
    87961 tatttatgct ttccatgata aactaaagtg accaaatcaa tgaggtggga aaatgattaa
    88021 actattagta gtagaagacg ataaagtatt atccaataat ataaaagaaa tactcgctgc
    88081 aatcggcgag ataacacaag tttatgatgg tagtgaagga ctgttcgagt tggaaagtgg
    88141 agtttatgat cttgcaatct tggatttgat gatgccggaa atgaatggat atgaagtgtt
    88201 agcaaaaata cggaaagaaa aaatacagac tccagtattg attttgactg caaaagatag
    88261 tttggaagat aagattgaag gatttgaacg aggtgcagat gattatctaa caaaaccttt
    88321 ctatcgagaa gaactgatca tgcgcgtgaa agcattattg aaacgctcgc taggtctctt
    88381 tgatgaaaat gttctgaagt ataaggatat cagttgtgat ttaaaaagca atgaagtctt
    88441 gtataaagaa caagttttgc ccatacaagg aaaagagttt gaattgctcg tttatttttt
    88501 acaaaataag ggaatcattt tgacaaaaga gcagatattt gaccggatct ggggatttga
    88561 ttctgatacg acgattacag ttattgaagt ttatatgagc aacttacgaa aacatctgcg
    88621 aagtactgga ttggataaag aaatcaaaac cttacgtaat gtcggttata tactgcaaag
    88681 ggtgtgatgc aatgaatgga gatattacac gaaagcaacg tagtcgcttt tttgcgacaa
    88741 atgtagcagc atttgctttt atcttttttc ttttgggatt cattacttta cagatattaa
    88801 accaatctgc atacagagaa acagatctgt cattagagaa aataaatttt gattctcgaa
    88861 cagtacaatt agagatacaa agatatcagc aaggcgatcc ctttttaaac ccgactcaaa
    88921 cggaacaaaa cagaacaaac atggaacagc cattaatgga accatcaaat gaccgtttta
    88981 acactcaagt gatcttgtgg tctaaaaatg gtgagatatt gaataaagca gctattggtg
    89041 ggcgtttcaa ccaaatcaaa gaattaaaaa tggatacgaa aaatctgaat accatccaaa
    89101 atattgaatt aaatgaagac agtggctcat ctgccctctc tttccgttcg attacgaaaa
    89161 aagcggagac gaatcaagat ggcgttgcct acatccaaat tttagaaaat acaaatcaaa
    89221 tcaagaattc attagcgact tttcgcacga ttatgatttt gtgtatgatt gttttttgga
    89281 ttctctctat tggtatcagt tattacttgt ctagtctaag catgcgccct attattcttt
    89341 cttggagaag acaaaaagaa tttgttgaga atgcttctca tgaattgcgc acaccgttaa
    89401 cgattattca aaatagtttg gaacatcttt ttaccaagcc taatcatact attattgaag
    89461 aatccgaaag catcgctcaa gcattgtcag agacgaggcg cctcactgga ttgacttcgg
    89521 atttgctgac aatcgctcgg aatgattctg atcagcagct gctttcaaag caaatgatca
    89581 atacacaaga atatatcgaa caacttgtta aaccatttca agagatggcc atcatagatg
    89641 gaaaagagtt tattctccat aacgatgcaa cattagaagt accaattgat gaaaaaaaaa
    89701 tccatcaagt tttggttatt ttgctggata atgcattaaa atatacgaat ccagaagata
    89761 agatcatttt ggattcatct gttaaccata aagaatggct gattcacgta aagaatactg
    89821 gcccttctat cagtgaagaa gatcaagcaa ggatttttga tcgattttat cgggaagatc
    89881 aatctcgggc gaaagagact ggcggatacg ggcttggctt ggcaattgca aaacaggtaa
    89941 tcgaacagca tcatggacag atttctgtat ccaattatct tccacaagga gtcgatttta
    90001 aagtccaatt gccgttgaag cctaaaaaat aatgcttatc cctaaaaaag ggctgtgaca
    90061 aaaatcttgt cacagccctt ttttgttgcg aataaacggt gttccagaag tagcttcttc
    90121 ggaaataagc cgaaatttct ttaagctgac cacttctgtc acaacctctt tagcttacgc
    90181 ttacgatttt ttattgcggt cttcaatgtg tacatcaccg cttgtgtcaa tatccaattc
    90241 ttctcggcta acatcttctg tcactttttc aacatcttca tgttcttctt tatgaatccc
    90301 aacttcttca cgaataactg tatgtttgct tacatctact tgctcttctt tgattgggat
    90361 agtaatcgtg tcatcttcca atttttcgtc tgttccctgt gaagattgat ctgttactgg
    90421 tttgcgttcg atgacgattt cttcacgttt gaccggtact tctaccgttt cagtatcgtt
    90481 gaccacatgt ttgtggatgt cgacttctcc agttgtcaca tcatgtgtat ttacatctaa
    90541 tttttcttct tttagttgaa ttgtatcatc atctagattt ggtttgcgat cgatgtcttc
    90601 agtcttgctt gtacccattg tatcagtctc gccaggtacg tccatcggat cagttcctgg
    90661 tgtcatattc aatggatccg ttgtattttg aaagcctgtc gtgccaccta cgtccattgg
    90721 atctgttcca gtgccagttt ctaatccagt ccccattgtg cttgctgggc taacagtatc
    90781 ttgtgtatcc attgtatctt ctgttgtttc tggctgacga tagcctttta ctgtgatgac
    90841 aaattttcca tcagaaatat ctcctctata ttgatataaa ggatcatttt ctggtgtagc
    90901 cgtctcagaa tcataggtat caaaagaaaa agtatctttt attttttccc ataaagaacg
    90961 gtcttcttca ccatccatcc gattttcatc tgtttcaaca tctataccag acaatccgtc
    91021 aaatcctagt ctagatgctg cttcatcagt agtataaata acgatatcat ctcgttcata
    91081 cccttcgtta cgcaaccgtt caattactga taatgcttct tccctcgtac tatagcttcc
    91141 gatgaccgtt aaatttttct cttccatgta aattcctcct agcaaataga tttatgattg
    91201 gtatattgtt acttataaat ttattctata ggagacgatt gttttctaca aatatttaga
    91261 ttggaatttg attaaaacaa tttaattgaa atttgaaaac atttatgtta atgaaagtca
    91321 tattctaata aaatgtcatt tgtttctata tgaattttta tgaaaaattt catataattt
    91381 ccactcgttg tgatggttta atttatccaa tccatcaata agcaaacaac ttatataaaa
    91441 aaggattaat tctcaatata attgctccta ccatagaata aatcttcatg taaagtaatg
    91501 aagtaccaac tatttctaat acttctttaa gaattatccc aacaatatcg ttagacaaac
    91561 cattattgaa taaatggaga gccaatcgat tctgaaaaaa tgttttctaa tttttcggaa
    91621 ataaaagaaa gagcctcctg ccattagaac gaaccaaatc catttcattg ctcatgaagt
    91681 aaccacatag ctaaaaagag gataaatcaa ttaaaagatc aaaagaatga ctgcactaat
    91741 aaaataacta aaaatgtatc ttcttacttc atatctttta gctagtactt agttaaaaat
    91801 ttgcttccta agatcaagac aaactgtcta tcttcatttg atgaaatgcg gtaaaatata
    91861 gaaaatgaac actattggag ggaatttgta atggacagca aaactagaaa acaactgcaa
    91921 gaaaagttaa agcaacgtac acacttgctc ccttccgtct ttcaacagtt tttagaagaa
    91981 aatcgttctt ccctttcatt aggcagtcgc tatgaatacg caaaagattt tcatctgttt
    92041 actgattatt tgagaaaagt attttctgaa gaagtggatg atcagctgat tatccattta
    92101 gaaaaacaag attatcagaa tttcttagct aaaatctatc gacatactcg ttcgttccaa
    92161 actacagcga accaagaaac ggagcaattg tttgaaaata gctattacgg gatttcaagg
    92221 aagcaatatt cattaaatca ttttttacga tttttgtatc aaaaaggact gattgaagaa
    92281 gagatttctc ttttgggtac aagtttaccg gaaacaacca aagcagctcc gcgatatatc
    92341 gatgcagaac ttttcaaaga ttttgagcat ttgtttatcc cgattgaagg ctttcaaaca
    92401 agcagagaag ctagctttga agaaaagaat agaccgagga accttgcaat tactgccatt
    92461 cttctgtatt gcggattaaa gatccatgaa gtagtagaac ttaaacgtaa agatgtatct
    92521 ttgaccaagc agattcttaa tctgaataga aaagaaaaca gactgaataa agtaccaata
    92581 ccaaaagaag cgatgccatt tctggagaac tattttaagt taacagcaca aaatacggac
    92641 gaaaattctt ttgtttttcg ttcctcacat gatcagcaaa tttctcctcg gactattcgc
    92701 caaattttga gcaaaattac agtatataaa aacgtatcgc cagaactatg cagaaaaaca
    92761 tatcgttatt atgcagaact atatttagat gatgctgacg cggtaaatta tttatgtggt
    92821 aatcgatacc tctcatccca ttcttttcaa gatatctggg aaaagatggt tgatttttct
    92881 tatcacgaat tagcggcatt cgttcgggtt tcttccatat aaaacaaatg tatgatcttt
    92941 ttttcacaat aaactatgtt attatataaa ggattatgtt acgattatct tgaatttgta
    93001 tattacttaa caaaagataa tggagtgtga atgatggaga ccataacgta ttcccctttg
    93061 aacttattta cgaattttca agaagctgca gctaactttt ccgagacccc gattatcttt
    93121 gatgagccac tttcagcttt cccagaatta ggacttgaaa caacttacaa aaaatgctgc
    93181 gaaagtgtgt tgaaacgggc ttatcaattg gctcatcttg gagtgaaatc cggagacaaa
    93241 gttattattt ataagagttc cgcttttgac acataccttc tagctgtttc tgtgtcttac
    93301 cttggtgcag tgcctgtcat gacttcttat catctaccaa caacaacaat ggaagtattt
    93361 atcgatcgtt tagaagaccc tttcatttta tttgatgatg agacaaaaaa gcgagtgaga
    93421 gaaatttcaa atggaacaaa atcaaaacag attcc
//
LOCUS       DAFEOM010000005        92608 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.005, whole genome shotgun sequence.
ACCESSION   DAFEOM010000005
VERSION     DAFEOM010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 92608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 92608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..92608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.005"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            160..840
                     /locus_tag="LP524_000392"
     CDS             160..840
                     /locus_tag="LP524_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBL2233474.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            930..1964
                     /locus_tag="LP524_000393"
     CDS             930..1964
                     /locus_tag="LP524_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBL2233475.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1978..2313
                     /locus_tag="LP524_000394"
     CDS             1978..2313
                     /locus_tag="LP524_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233476.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2297..4777
                     /locus_tag="LP524_000395"
     CDS             2297..4777
                     /locus_tag="LP524_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233477.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            4833..6392
                     /locus_tag="LP524_000396"
     CDS             4833..6392
                     /locus_tag="LP524_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2233478.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6389..7753
                     /locus_tag="LP524_000397"
     CDS             6389..7753
                     /locus_tag="LP524_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL2233479.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7924..8994
                     /locus_tag="LP524_000398"
     CDS             7924..8994
                     /locus_tag="LP524_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2233480.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLVQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            9009..9677
                     /locus_tag="LP524_000399"
     CDS             9009..9677
                     /locus_tag="LP524_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2233481.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9697..10275
                     /locus_tag="LP524_000400"
     CDS             9697..10275
                     /locus_tag="LP524_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL2233482.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10676..11587
                     /locus_tag="LP524_000401"
     CDS             10676..11587
                     /locus_tag="LP524_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBL2233483.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11965..12882)
                     /locus_tag="LP524_000402"
     CDS             complement(11965..12882)
                     /locus_tag="LP524_000402"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HBL2233484.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            13084..13521
                     /gene="zapA"
                     /locus_tag="LP524_000403"
     CDS             13084..13521
                     /gene="zapA"
                     /locus_tag="LP524_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBL2233485.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13579..14127
                     /locus_tag="LP524_000404"
     CDS             13579..14127
                     /locus_tag="LP524_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HBL2233486.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            14242..16602
                     /locus_tag="LP524_000405"
     CDS             14242..16602
                     /locus_tag="LP524_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBL2233487.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16689..17003
                     /gene="trxA"
                     /locus_tag="LP524_000406"
     CDS             16689..17003
                     /gene="trxA"
                     /locus_tag="LP524_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBL2233488.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            17185..18963
                     /gene="uvrC"
                     /locus_tag="LP524_000407"
     CDS             17185..18963
                     /gene="uvrC"
                     /locus_tag="LP524_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBL2233489.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            19108..20007
                     /locus_tag="LP524_000408"
     CDS             19108..20007
                     /locus_tag="LP524_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2233490.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            20000..21262
                     /locus_tag="LP524_000409"
     CDS             20000..21262
                     /locus_tag="LP524_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2233491.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21423..23321
                     /locus_tag="LP524_000410"
     CDS             21423..23321
                     /locus_tag="LP524_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HBL2233492.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(23651..24370)
                     /locus_tag="LP524_000411"
     CDS             complement(23651..24370)
                     /locus_tag="LP524_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBL2233493.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            24706..25038
                     /locus_tag="LP524_000412"
     CDS             24706..25038
                     /locus_tag="LP524_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233494.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            25025..27028
                     /locus_tag="LP524_000413"
     CDS             25025..27028
                     /locus_tag="LP524_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBL2233495.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            27047..27517
                     /locus_tag="LP524_000414"
     CDS             27047..27517
                     /locus_tag="LP524_000414"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBL2233496.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27538..28122
                     /locus_tag="LP524_000415"
     CDS             27538..28122
                     /locus_tag="LP524_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HBL2233497.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            28137..29123
                     /locus_tag="LP524_000416"
     CDS             28137..29123
                     /locus_tag="LP524_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HBL2233498.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            29113..29424
                     /locus_tag="LP524_000417"
     CDS             29113..29424
                     /locus_tag="LP524_000417"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBL2233499.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29446..31227
                     /locus_tag="LP524_000418"
     CDS             29446..31227
                     /locus_tag="LP524_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBL2233500.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            31220..32596
                     /locus_tag="LP524_000419"
     CDS             31220..32596
                     /locus_tag="LP524_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBL2233501.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32603..33238
                     /locus_tag="LP524_000420"
     CDS             32603..33238
                     /locus_tag="LP524_000420"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBL2233502.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            33268..34623
                     /locus_tag="LP524_000421"
     CDS             33268..34623
                     /locus_tag="LP524_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBL2233503.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(34815..36209)
                     /locus_tag="LP524_000422"
     CDS             complement(34815..36209)
                     /locus_tag="LP524_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBL2233504.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            36301..37272
                     /locus_tag="LP524_000423"
     CDS             36301..37272
                     /locus_tag="LP524_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBL2233505.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            37269..38051
                     /locus_tag="LP524_000424"
     CDS             37269..38051
                     /locus_tag="LP524_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBL2233506.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(38220..38591)
                     /locus_tag="LP524_000425"
     CDS             complement(38220..38591)
                     /locus_tag="LP524_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL2233507.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            38725..39168
                     /locus_tag="LP524_000426"
     CDS             38725..39168
                     /locus_tag="LP524_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBL2233508.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(39211..40020)
                     /gene="yidA"
                     /locus_tag="LP524_000427"
     CDS             complement(39211..40020)
                     /gene="yidA"
                     /locus_tag="LP524_000427"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBL2233509.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(40039..41406)
                     /locus_tag="LP524_000428"
     CDS             complement(40039..41406)
                     /locus_tag="LP524_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL2233510.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEYIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(41390..42220)
                     /locus_tag="LP524_000429"
     CDS             complement(41390..42220)
                     /locus_tag="LP524_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBL2233511.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            42383..42820
                     /locus_tag="LP524_000430"
     CDS             42383..42820
                     /locus_tag="LP524_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBL2233512.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            42896..43516
                     /locus_tag="LP524_000431"
     CDS             42896..43516
                     /locus_tag="LP524_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HBL2233513.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            43705..44211
                     /locus_tag="LP524_000432"
     CDS             43705..44211
                     /locus_tag="LP524_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HBL2233514.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            44228..45127
                     /locus_tag="LP524_000433"
     CDS             44228..45127
                     /locus_tag="LP524_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBL2233515.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            45214..46098
                     /locus_tag="LP524_000434"
     CDS             45214..46098
                     /locus_tag="LP524_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBL2233516.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(46290..46361)
                     /locus_tag="LP524_000435"
     tRNA            complement(46290..46361)
                     /locus_tag="LP524_000435"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46326..46328),aa:Arg,seq:ccg)
     gene            46421..47755
                     /gene="rpoN"
                     /locus_tag="LP524_000436"
     CDS             46421..47755
                     /gene="rpoN"
                     /locus_tag="LP524_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBL2233517.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            47904..48995
                     /locus_tag="LP524_000437"
     CDS             47904..48995
                     /locus_tag="LP524_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HBL2233518.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            49036..50037
                     /gene="gap"
                     /locus_tag="LP524_000438"
     CDS             49036..50037
                     /gene="gap"
                     /locus_tag="LP524_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBL2233519.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            50147..51337
                     /locus_tag="LP524_000439"
     CDS             50147..51337
                     /locus_tag="LP524_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBL2233520.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            51441..52196
                     /locus_tag="LP524_000440"
     CDS             51441..52196
                     /locus_tag="LP524_000440"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBL2233521.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            52312..53610
                     /gene="eno"
                     /locus_tag="LP524_000441"
     CDS             52312..53610
                     /gene="eno"
                     /locus_tag="LP524_000441"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBL2233522.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            54010..55149
                     /locus_tag="LP524_000442"
     CDS             54010..55149
                     /locus_tag="LP524_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HBL2233523.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEELTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(55216..55791)
                     /locus_tag="LP524_000443"
     CDS             complement(55216..55791)
                     /locus_tag="LP524_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL2233524.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(55796..56770)
                     /locus_tag="LP524_000444"
     CDS             complement(55796..56770)
                     /locus_tag="LP524_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBL2233525.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(56794..56838)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            57053..58021
                     /locus_tag="LP524_000445"
     CDS             57053..58021
                     /locus_tag="LP524_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL2233526.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            58134..58412
                     /locus_tag="LP524_000446"
     CDS             58134..58412
                     /locus_tag="LP524_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBL2233527.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCTYAKKEFEKKPEYQDVLRQS"
     gene            58530..58766
                     /gene="secG"
                     /locus_tag="LP524_000447"
     CDS             58530..58766
                     /gene="secG"
                     /locus_tag="LP524_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBL2233528.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            58869..59624
                     /locus_tag="LP524_000448"
     CDS             58869..59624
                     /locus_tag="LP524_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBL2233529.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            59627..61984
                     /gene="rnr"
                     /locus_tag="LP524_000449"
     CDS             59627..61984
                     /gene="rnr"
                     /locus_tag="LP524_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBL2233530.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            62006..62470
                     /gene="smpB"
                     /locus_tag="LP524_000450"
     CDS             62006..62470
                     /gene="smpB"
                     /locus_tag="LP524_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBL2233531.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            62772..63491
                     /gene="atpB"
                     /locus_tag="LP524_000451"
     CDS             62772..63491
                     /gene="atpB"
                     /locus_tag="LP524_000451"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBL2233532.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            63544..63759
                     /gene="atpE"
                     /locus_tag="LP524_000452"
     CDS             63544..63759
                     /gene="atpE"
                     /locus_tag="LP524_000452"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBL2233533.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            63850..64374
                     /gene="atpF"
                     /locus_tag="LP524_000453"
     CDS             63850..64374
                     /gene="atpF"
                     /locus_tag="LP524_000453"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBL2233534.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            64361..64903
                     /locus_tag="LP524_000454"
     CDS             64361..64903
                     /locus_tag="LP524_000454"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBL2233535.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            64926..66482
                     /locus_tag="LP524_000455"
     CDS             64926..66482
                     /locus_tag="LP524_000455"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBL2233536.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            66496..67398
                     /locus_tag="LP524_000456"
     CDS             66496..67398
                     /locus_tag="LP524_000456"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBL2233537.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            67421..68827
                     /gene="atpD"
                     /locus_tag="LP524_000457"
     CDS             67421..68827
                     /gene="atpD"
                     /locus_tag="LP524_000457"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBL2233538.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            68841..69257
                     /locus_tag="LP524_000458"
     CDS             68841..69257
                     /locus_tag="LP524_000458"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBL2233539.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERDIDVSRAERAKQRA
                     ERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            69726..69956
                     /locus_tag="LP524_000459"
     CDS             69726..69956
                     /locus_tag="LP524_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HBL2233540.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            70053..71354
                     /gene="murA"
                     /locus_tag="LP524_000460"
     CDS             70053..71354
                     /gene="murA"
                     /locus_tag="LP524_000460"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBL2233541.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            71367..71543
                     /locus_tag="LP524_000461"
     CDS             71367..71543
                     /locus_tag="LP524_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBL2233542.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            71678..71974
                     /gene="yidD"
                     /locus_tag="LP524_000462"
     CDS             71678..71974
                     /gene="yidD"
                     /locus_tag="LP524_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBL2233543.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            72158..72625
                     /locus_tag="LP524_000463"
     CDS             72158..72625
                     /locus_tag="LP524_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2233544.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            72710..73594
                     /locus_tag="LP524_000464"
     CDS             72710..73594
                     /locus_tag="LP524_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL2233545.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            73739..73945
                     /locus_tag="LP524_000465"
     CDS             73739..73945
                     /locus_tag="LP524_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HBL2233546.1"
                     /translation="MRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGPKEYEIFFGE
                     EDFGKEKSLENAIETVIRHWNLHE"
     gene            74033..74113
                     /locus_tag="LP524_000466"
     tRNA            74033..74113
                     /locus_tag="LP524_000466"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:74067..74069,aa:Tyr,seq:gta)
     gene            74120..74191
                     /locus_tag="LP524_000467"
     tRNA            74120..74191
                     /locus_tag="LP524_000467"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:74152..74154,aa:Gln,seq:ttg)
     gene            74316..74486
                     /locus_tag="LP524_000468"
     CDS             74316..74486
                     /locus_tag="LP524_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233547.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            74706..75413
                     /locus_tag="LP524_000469"
     CDS             74706..75413
                     /locus_tag="LP524_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL2233548.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(75473..76117)
                     /locus_tag="LP524_000470"
     CDS             complement(75473..76117)
                     /locus_tag="LP524_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBL2233549.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            76244..76531
                     /locus_tag="LP524_000471"
     CDS             76244..76531
                     /locus_tag="LP524_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233550.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            76639..76959
                     /locus_tag="LP524_000472"
     CDS             76639..76959
                     /locus_tag="LP524_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2233551.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            77040..78353
                     /locus_tag="LP524_000473"
     CDS             77040..78353
                     /locus_tag="LP524_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBL2233552.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            78350..79069
                     /locus_tag="LP524_000474"
     CDS             78350..79069
                     /locus_tag="LP524_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBL2233553.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            79197..79748
                     /gene="raiA"
                     /locus_tag="LP524_000475"
     CDS             79197..79748
                     /gene="raiA"
                     /locus_tag="LP524_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBL2233554.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            79970..82504
                     /gene="secA"
                     /locus_tag="LP524_000476"
     CDS             79970..82504
                     /gene="secA"
                     /locus_tag="LP524_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBL2233555.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            82630..83731
                     /gene="prfB"
                     /locus_tag="LP524_000477"
     CDS             join(82630..82701,82703..83731)
                     /gene="prfB"
                     /locus_tag="LP524_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBL2233556.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            83836..84522
                     /gene="ftsE"
                     /locus_tag="LP524_000478"
     CDS             83836..84522
                     /gene="ftsE"
                     /locus_tag="LP524_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBL2233557.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            84506..85399
                     /locus_tag="LP524_000479"
     CDS             84506..85399
                     /locus_tag="LP524_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2233558.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            85551..86999
                     /gene="cls"
                     /locus_tag="LP524_000480"
     CDS             85551..86999
                     /gene="cls"
                     /locus_tag="LP524_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBL2233559.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            87336..88193
                     /locus_tag="LP524_000481"
     CDS             87336..88193
                     /locus_tag="LP524_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBL2233560.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            88308..89228
                     /gene="pstC"
                     /locus_tag="LP524_000482"
     CDS             88308..89228
                     /gene="pstC"
                     /locus_tag="LP524_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBL2233561.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            89228..90112
                     /gene="pstA"
                     /locus_tag="LP524_000483"
     CDS             89228..90112
                     /gene="pstA"
                     /locus_tag="LP524_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBL2233562.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            90127..90933
                     /gene="pstB"
                     /locus_tag="LP524_000484"
     CDS             90127..90933
                     /gene="pstB"
                     /locus_tag="LP524_000484"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBL2233563.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            90949..91707
                     /locus_tag="LP524_000485"
     CDS             90949..91707
                     /locus_tag="LP524_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBL2233564.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            91720..92397
                     /gene="phoU"
                     /locus_tag="LP524_000486"
     CDS             91720..92397
                     /gene="phoU"
                     /locus_tag="LP524_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBL2233565.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
BASE COUNT        30683 a        16126 c        19802 g        25997 t
ORIGIN      
        1 ttaataaaaa aggaagaata ttcatgtaaa cgattttatt gtttttttct cttttataca
       61 aaaagggtca taatttcaag taagcggatg aattcatgag gattttttgc atactgtctc
      121 tactatactt aggtcactaa gaaaaaggag tgatttatta tgaaaaagaa catgattggg
      181 acattattac tttcaacact tcttctaggc ggcttggcaa caccagcttt tgctgagggt
      241 caagcgactt caaaaggaga tattaccttt actgaaccaa caaataccgt ggagcctctt
      301 aaccctactg atccttcaaa accagtagag ccagcagatc cggaaaatcc agcaactggc
      361 cagacaggtc ctttaacact ggatgtagtt cccgaattgc catttggtac acacgagatt
      421 gaaagcggaa caaaaacgta tcaagtagac gcttcaaaaa acgatacacc ttatctccaa
      481 gtatcagatc gtcgaggtgt aggtgcagat ggccaagcac aagggtggaa cgtgacagtt
      541 tcggtttcag attttgtaaa cggttcacaa gttttgcaag gcgctgaatt agattttgga
      601 acatccactg ttaaaagtac gtcagataac gaatccactg gaccaactag tcaaactgta
      661 actgggttga gtaaagcaag tgcagcgaca ccaatattta cagcagcaaa agatcaaggt
      721 ctgggaacat ggctttctgt ttatgatcca gcaaatatca cactgaaagt gccgaaagca
      781 gcagctggaa cattcacggc tgatttgact tggaacctcg tggctggccc agtagcatag
      841 agtatgaaat aaattcaaca aactgcggtg aaaatactat aggtgaagga ttttttaccg
      901 cagttgttgt tgaaaggatg tggtggacaa tgaatcaaag taaacgactg tttgtttcgt
      961 ttttttctat tttaagtcta tttttcattt ttccgtcaat cagccaagca aaggattcag
     1021 caggagactt tggaatcaaa ccggtatttc cagagaacca aatagataaa gcaatcggtt
     1081 attttgattt attagtggca ccggaacaaa atcagacact agaagtaatt atcagcaatt
     1141 cttcagatga agaacgaaca tttgaggtct ctgtcaatcc ggcggtaacg agtgatggtg
     1201 gaacgatcga ttatagtcaa aaaaacccaa cgttagatga gacattgccg tttgatgtcc
     1261 gtgacgtttt gctcattgcg aaaaaggaaa tcaatgtatc agcacatgca gaaacaactg
     1321 tacctataga agtgaaaatc ccagcaaaaa gctttaaggg acgtgtatta gcgggtattc
     1381 atgtttcacc taaagaagaa gctgaaacag aaaatgctac agaaggagca caaatcaaaa
     1441 atcgtattgc ttataatctc gcagtcgttt tacaagaaag tcaagagacg atagaaccgg
     1501 atctgaaact tttatcagga gatttggacg aggtcaacgc aaagccaact gttcagcttc
     1561 gtttccaaaa tcctcagcct aggattatca gcaatttgat atttacgagc aagatctttt
     1621 atgaaaatca gctttatatt gaaaatacgt ctaatgcatt tttagttgca ccaaattcga
     1681 attttcactt gaatcttgat ttagctggtg ataaagcaaa agccggcgat taccgcgcag
     1741 aaatcatagc aaaatcaggc gacagtaatg aatggcgatt tacacaaaac tttacgatca
     1801 aaaaagaaaa agcacaaaaa gtaaatgaaa attccgtttt tgcagtacaa gaacaatctt
     1861 tcccatggct ttatgcagga ttggttgctg cagttctagt gttatggttg attttgctgt
     1921 ttttgtgggt ccacaagaaa aagaaaaaag gagaaacaac atgatgaggt gatttcgttg
     1981 aaaacatcga tcttattggg agggattatt gcaagtttgc tgttcttcag ctggcaagag
     2041 ccagtccaag cagataccaa aagttcaggt acgattacat tttatgatct aagtccaatt
     2101 ccgcatccaa gtggttcagt caatggcgca tcaaaacagc agacacagct accgatttat
     2161 aaagaatcta atcaacatgg cgtattcccg gagacgaatg aacaaaagat ccgttttctt
     2221 tatttgatca ttggtataga attatggctt cttgcgtggt tgttgttatg gagaagaaag
     2281 gaaaaggagg atgaacatgt tgctacaaaa taaattttca ttttttcgct ccttcttttt
     2341 tacttcgctt tttgttttaa gtatatttat cttttcacct tcggcagaag ctgttggagc
     2401 aacgcctcct gccatttctt atgatccata cacgtatgaa aatatcgtac ctccaaatgg
     2461 tggacgtagt gcagtcaatg gcgggaagct tgtccaacta actggggcta accaattgcc
     2521 taaattgact gctacttatt tgaacgatgg ttcaaatgat ccttggaaag gtggcacggt
     2581 ccgttttcat tgggaaagcc atgtgaaact aacaaatgta cttgatttgg tgaatatcac
     2641 tgcacaagga tttgcatttg tggcgatcat caatttgccg gaacatgtat caagcagtga
     2701 tgtattaaaa gcaattgact ggaacaacgc ctacttgtat attgccggtg cccccatcaa
     2761 ggttactagc ggaagtttat tatcagcagg aagccatacc ttgcgttttg ggatggggtc
     2821 gtgggataca aatactctat tgacagatat tattgctatt attacgcgag gcgaattcaa
     2881 cgtggacgat atcccgcttc aatttgatgt tacagctaat gtcgcgaaga tgacagaaaa
     2941 cggagataaa tatgatacga gtccaaacaa aattttgacc aagggtaaat tacaaccttc
     3001 tatgtctaaa aaggccgttt tttctgttga tttttatgat tcgaataata ttatccaaga
     3061 tcgtatgttc aattcgattt tagctccagc tgcaggtaga ttttatccta aattaactgg
     3121 cggaaacttg aatccaggag catatgcatt ttctgcaagt gcagagataa atacgtggaa
     3181 tagttacatc tcaccaagtg attcaacagg aaaatataat acatttgctg ataccacaga
     3241 atacgtggat ggaaataacc agaaccttcc aggctctacc aatagtcgga cattgacgat
     3301 ggggctgact gaaggacgtt ttgatcagtt gccatttagt cgctttgatc gcgtagtcaa
     3361 ctattttaca ggaaaaaacg ttacacaagg agcaatactg actcatactc cagcaagcac
     3421 aagtggattg aatcagacag ccaatatcgt gtacagtggg aaagatgcgc aaggcggatt
     3481 gctttctcct gttgctttaa aggtattgga gcagtacaag ctagacggaa cggttttacc
     3541 aacaaactta acagccgata gtcagtgggg aaagctcctt tcttctgcat cgtaccaagt
     3601 cacggccaat tggtcagcaa ctgaactaaa aaagggttcg atccattaca ggctttattc
     3661 gaaaggaacc caaaaattag cgacaggatg gacagatcaa cttttccaaa cgatccaatc
     3721 aaataacggg ggtgtaaata cagctacttc tacactacct gcattggctt cagggcaata
     3781 ttattttgat tatcgcttgg tggacgatgt acttactaac atgtatccgt cttttgcgta
     3841 caaatggcag tctgaacaag ctggaatcac cactcttcct caaataacag tggctaattt
     3901 tccatctgtg agtgcagata gtcagctaaa aaaccttagc agacaacctg agacaggaga
     3961 tcccctgtct gctctatcag gagatacgat tcaagaaaat gtgacattca cgttaacaaa
     4021 atctggggat gcgataaccg acaaaaaaat cacgatctca cttcctgaaa atactactta
     4081 tatcaatggt tccttaaaat taaatggtac aactatcgca gataccggca ttcaacaagg
     4141 agtttctgtc ccagcagatc tattgagcaa aataggagat accattcatc ttacttacaa
     4201 ctatcaatta aatactgtag atacttcttt acaatccgtc agcatactga caaaggcagc
     4261 tgtactttcc agcaatatta cattagcaga tggtgctaag cttcctaacc cagttgtcca
     4321 aacatctgct aaaacaattc ttgtccccaa acaagaatta acattagtta atgtaccaga
     4381 tgattttaca tttggtaacg atctccccaa accgttgaag acaagttatt atgaatcaaa
     4441 aggcgatttt tcttttgatg tgcgggatac tcggcttcca agcacgtctc cttggcaact
     4501 gacaggtaca ttgacttctt tatttaagaa tgatcaaggt caagaattaa gcggaacaaa
     4561 attatacttc aatcattctg gatcaaagca attgattcag caaggtcaaa acacgttgat
     4621 ttatgagagt gatggaacag ctaaaggaga ggttctcgtt gactttcccg ataccgatgg
     4681 tttactctta gaggtaaata gcagtacgaa tgctcaacct ggcgcaacgt atcaaggcat
     4741 ggtcacatgg gaactgacag ctgggccaac cagctaagtg attagaattt gtatggatct
     4801 gtgataactg tcacagatcc tctttttcaa ggatgagaag gaaacttctg ctgttaaaat
     4861 cagaaatgct tgaaggagtg gacttggaac ttttcagttt gcttgaaaaa aatgaacaac
     4921 ggcaaatcaa acttttccaa cttttattag aggaaaaagg agaggtttcc gttaataacg
     4981 tacttacccg cttatcagtt gatcgggcta ccttgaaaga agatctgttt tatttacaag
     5041 ctaacttgga aaatatcaaa caattggaaa ttgttatcca agaagggaat attttattaa
     5101 tcagacatgg aaatatagca gcagtcgatg tttattatca ttgttacttg cgctattcta
     5161 caaaatatca gatacttctt tatctgttgg aaaatggctc atttgatcgg cagaaacttg
     5221 tcatggaact taatatcagt tcggctactt tgtctcgcag agtgaaggaa ttaaatgatt
     5281 ctttaaatga gtttcatctg aaaataaaaa gcgggcggct gaccggtcca gaaagtcaaa
     5341 ttcgttattt ttatttccaa ttattttggt ttggacgtcc ctatcttgaa aacaaaaaag
     5401 aatacagtga tttatcagga aacacttttt tagatctgct gcaaaaagag tttagttttg
     5461 ctattacaga agaaggacga ttgaaatttg aaatatggct ttccattatg aagaagcgaa
     5521 tgcgctgtac gaaaaaatcc aaaccagatt atagcaatct tcctgttgga ttgtttcaaa
     5581 cgacacaatt tttattggac ctccatacat ttttgacccg ttacttcttt caatatgcct
     5641 ttccttggaa tcaaaatgaa acgttggttt tttatctttt catgatcagt aactttacat
     5701 tagatacaca taactcgcat gtccaggagt ttttgataag cgaaaaatac aaagacgacg
     5761 tagtagctca attgaatcga acgtttaaac aatcgataga agaatatttc tctttttatg
     5821 tttttccaga agagatttcg caaaagattg cgttgacagt gatgcaaaat cacgctcatt
     5881 ttgcctattt tagaggatgg atgaatattt ttggccaaga aggattaaaa cagcggttaa
     5941 ctgaagtaga agataaacaa ttcctggttt tatgtgaaag attggtcaga caaacatttg
     6001 ctattgttga ttttactgaa gtagacgagg aaaatgcccg ctttgaatta tttgctcgct
     6061 atgcatctat tttacggacg ctgatcaagc agttagaatt acgtctgcaa attgcttatg
     6121 atcttcctta tgaaaggcta atggcggata tcgtgaaaga aatcatccag gaacaattgc
     6181 tgaatacgat caaatatgat ttagtcgaat acgagaaaga aaagcagtat gatatcattt
     6241 tgactagcca attgaaagag tacccgtcac agaagtccgc taaagttttt gtattcacta
     6301 gcaacgaata taattatgat ttcccctatt tgaaccaatt tttaaaagaa tgttatttag
     6361 aaaaattgaa tttaaggatg aataaaacat gaaaatcgtg atcattggtg cttcccatga
     6421 aggcatcaca gctgccctaa atctaagaaa acttcaaccg aaagcagaag ttttactgat
     6481 cgatgaagat cataaagatg gactaggcta tgtttcaaat ggaatcaacc tttatttaaa
     6541 aggaaagata cgtagtttag ctgaagttgc ccataacatg cggactcttc aagaaagtgg
     6601 ggcaaaactg atcaccgaat ggagagtaac tgaacttgat ccagacaaac atcagctcat
     6661 cctcgcatcc aaagaaggaa aaaacgagac cataacctat gataaattaa tcgtagcgac
     6721 aggttcctct cctgtcacct tgtataaaca aattgaagct gaaaatgtct acacctataa
     6781 aaatctagtg caatctaagc aagtacttgc cgctttaaaa gaagcaaaag aagtagtgat
     6841 cttcggtgct ggttacattg gattggaatt agcagatgct ttgagaaata aaggatacat
     6901 tattcatcta gttgattata tgccgaatgt tttatcaaga tatttcgata aggatatgat
     6961 caacagtttt caaaatcagc tgcaaactaa gcaaataaat ttttatccaa atgaattctt
     7021 gatcgattgg aaaaaatcag aagagaaagt agtatccgta caacttctgt ctcaaacaat
     7081 aaaggcagat atggtcattt tttctgccca aactcgtcct aatacgacat tgttaaaaga
     7141 aaaagtggct ttgtatgagg atgagactgt gatagtcaat gaatatctgc agacaagtga
     7201 tccggacatc tatgcgattg gagatatcgt cccagtcagt tttgacaaaa ataaacggca
     7261 tctcttttta cctttagtta ctcgagctgt acatatggca agagcagtag cgctaacttt
     7321 gagtggtcag ccaaccgctt acgacctcag acaaaagata acagcagcag tcattataga
     7381 ctacttttta ggaactgtag ggctgacaga agatgaagcg ccattcttgg aacaaaatac
     7441 cggttcgtgt tcaggagaat ttgatctatt tccacaatac gacgaagaaa acaaaacagt
     7501 aaatgcaaag ctgatctacc atccggatac attagaaata attggcggac agctgataag
     7561 ccaagaattc ttattatcag atttgaacct gcttgcggat atcgtgaaac acaaaactac
     7621 gattccacag ctagcagttg aggattttgg atttctgtct gaatacacct ctcgttttca
     7681 ctacctcaat gagttagctt ttaaagtact agctaagaga gcaaatagca atagagtaga
     7741 taaaagactg tgagaaatat cacggtcttt tattgtgtgg ggatataata aatattaatc
     7801 aatcgagaat aaaaaattca tataatgaaa tagcggaaga attttttgag cataatattc
     7861 gccaaaaatt tatcaagtga taaaattgtt gtataaaatg attatttcag gaggaggagt
     7921 taaatgtttt tagcattaaa tgaaattatg cattcaaagt tacgttatgc gttagttgca
     7981 ggggtgatgt ttttgatcgc ctacctggta ttttttttga ctgggttagc ttatggctta
     8041 gttcaagata atcgaacagc agtcgacaag tgggaggcag attcgattgt gctttcgaaa
     8101 gatgcaaatt caaatctagg gatgtccatg attactaaga agatagcaga agaagtcgaa
     8161 ggtgggaaag tggcgtatct tgcccaaaca cctggagtcg tgactagtaa agacagtaca
     8221 gaagaaggaa aaatcaatgt tagcttcttc ggtatcgata aaaatcaatt catcatgccc
     8281 aatcttgttg aaggaaaagc ttttgataac gatgatgagg cagtaggcga tattagtttg
     8341 aaagaagaat atggtctagc cataggagat acagttaagt tatctggtag tgacaagacg
     8401 tttaaactaa ctggctttac ggatcatgcc aaattcaatg tctcaccagt tctctataca
     8461 accatcaatg cttaccaaga aatccgcttc gaaaaagaag atacaagtga aaatgcacgg
     8521 attaacgcaa tagttgtcag aggtaaaatc agtaatttac ctgaagattt agaacagatc
     8581 aaaatttcta aattcatcaa tgaattacct ggatataatg cgcaagtatt gacttttgga
     8641 tttatgatcg gatttttgat cgttattgcg gctattgtga tcgggatttt catttatgta
     8701 ctgacgatgc aaaagattaa tatctttggt gtaatgaaag cacaagggat cacaggagga
     8761 tttattgcgc gttctgtagt ggcacaaaca ttcattttat catttgttgg tattcttctt
     8821 ggtctattag gaacagtggg aacatccctt gttctacctg atgcggttcc attccaaagc
     8881 aattggctgt tttttggcgt cattagcttg ttgatgttag tagtagctgt gttaggcgca
     8941 ctattttctg ttcggacaat cgtaaaaatc gatccgttga aggcaattgg ataagaagga
     9001 ggaagaacat gaaagcaata gaatttaaag aagtagaaaa aagtttccaa gatggcgacc
     9061 aagtagttaa agcattgaaa gaaacaacca tgagtatcga taaaggggaa tttgttgcaa
     9121 tcatcggtcc ttctggttca ggtaaaagta cttttttgac tttggcaggc ggattgcaga
     9181 cgcctagcag tggctctgta tttatcaatg atcaagaatt cagtaatcaa aaagaaagcg
     9241 aacgcgttaa actacgtttt aaagaaattg gctttatttt gcaagcttcc aatctggttc
     9301 cttttctgac agtaaaaaag cagttattat tagtagataa gattaaacat gaacatcgtc
     9361 aaaaagaagc aaacgaatta tttgagcagc ttggcgtgga taagttgatc aacaagtatc
     9421 cagaagaact atctggtgga gaacggcaaa gggtcgcaat cgctcgtgca ttgtacaatg
     9481 atccgtccat tattctagca gatgaaccaa ctgctagttt ggattcagaa aaggcacgag
     9541 aagtagtcga tatcttggca aaagaatcaa aagacaaaaa taaagcaacg atcatggtta
     9601 ctcacgatac acggctgatt gacaaatgcg atagaatatt tatgatggaa gatggtgtat
     9661 tgaaagaaaa agaataacca aaaagcaggt ggaaaaatgg tccatcgata caacaaagat
     9721 acacagcaaa aaattttgaa agaagcgact gacttgttta tgtccaaggg atatctcggc
     9781 acttcaacaa gagaaatcgc acaaaaagtt gggatcaccc agcccaatct ctatcattat
     9841 ttcggtgata aagaaaaact atatacggca gttttagaaa accacttaaa agatgttgga
     9901 aaagctttga gagaaatcgt tcaaacaagc gaaacgggat ttcaagtaac cttgacaaaa
     9961 atggcgcagt ttttgattga gacacatctg gtcgatttgt ttctgatgct tcacgatctt
    10021 gaaagcaatc tttcaaagga aacaagagat catcttttct ttctatggaa gaaaaattat
    10081 cgtgaacctt ttgaagaaat cttttcagca aaccaatctg tgctaagaaa tgggatttca
    10141 aaagaaatcg cagcaaggca ttttttctta gtgttagcac cttacatcac tcggtcgaaa
    10201 agacctgtgg aagaaccgct aaatgtggcg caattgatcg atttatattt gaatggtgta
    10261 ttaggagaag cgtaaagctg ttatataggg agaaaatagt taggtgaaga agcaagatat
    10321 gacgaagatc gttatatctt gcttctttat ataggtagtt tttcataaat acaaatttgt
    10381 tattctctca tgattatcag ggtatatttt tatttattat gtcggtgttc caggaagtgt
    10441 tttaatttaa taacgaagaa attaattgtt tgtagaatga aggaaactat ttattgggta
    10501 gatgaatcac cacgaatagt caaaaggaga tttcaggata tgccaaaaat gaaaaaaatc
    10561 attttagcag taatgttatt ttctacggtt atatctttta cagccccttt tttgcttact
    10621 taagctgatt ttaagttttg gcccttagtc tggatctacg aggtgagagt caagaatgaa
    10681 aaaaataatt ggagcttttg tatttgcaat cattatcatt attatctgtt ttcaaataga
    10741 tgtttttggt caaaaaaaga tagttggaca aacggtcagc agtacgaaca gtacggttac
    10801 aaagacaaag gaatcatttg atgatacaga acaagagaca ttaatcaaaa tcggaaaaaa
    10861 tgaccgtaca ctatatacag atgactcgct aactacccca cttgcaacta taaacggcgg
    10921 agagctggcg gagtttcttt ctgaaacaaa aaatgcttat cagatcaaga caaatgatgg
    10981 ctatactgga tatttagccc tcgaagatgg aaccaaagtc acaaagaata tacaaacaaa
    11041 accggaagag ctatctgatg cagtgattgt attagatcct gggcatggcg gaaatgatac
    11101 tggggcatta agcaatgatg aacaaacaga agaaaaagat ataacattaa gtacagccat
    11161 aaaagtaaaa aaggcgctag aggatgctgg agcaacggtt tatttgaccc atactacaga
    11221 tgaattagta cagcttggag atatatgcga ctacagtgaa gaaaaaaagg cagatgtctt
    11281 tatcagttta cacgcagact ctacggaata tgcgaatgaa gcaactggaa tcacgacata
    11341 ttattactat ggacaagagg aaacattagc acagacaatt gctgactcct tcactgatct
    11401 gcctctggat tcaagaggaa tctccactgg gaactatcaa gttcttcgag aaaatcttca
    11461 accttctatt ttaatcgaga tgggttatat gaataatgat agtgatttaa aggaattagt
    11521 aacagacagt tatcagcagc agattgctgc aagtttgaca caagcattga ccacttattt
    11581 tcagtaataa ataagcccag ccaaacaaaa atagctcctc atatttttcg tttggccggg
    11641 cttattatcg cagatgcaag cttttaaaca ccgtttaagg aagaggtcgt gagcctgccg
    11701 ttcaactctg agtcacaagg agcaatttca aaaaacagct tctcatgttt gatgactatt
    11761 gtgacttgtg accgaggagt tggcatgaga acaccgttta agaaaaaggt tgtgaaatca
    11821 acgttttgtt ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt
    11881 tgttgagcta ataccgagga tcagatgatt gaacaccgtt tattcggaac aagggactgt
    11941 gacatgactt ttgtcacagt tccattattt ttttagtagc ttcaaagctt tttctgtatt
    12001 ggcaaaatga agtttggcat atttttcaag cataggcatg ccgcctttct ccaatagttt
    12061 tgccgctact tggtcagatt tcgttccagc tcctgacgga agggtgattc cagcttcagc
    12121 tgatgctttg tctaactctt ccaaaaagct ggcacgactg atgatagaag cagcagcaac
    12181 agctaaatga tattgttctc ctttagtaac gaagtacagt ttttcttgta attgattttt
    12241 ttctaaacgc gcatattttt taaaatttgc ttccggcgta aactgatcga tcaaaatcgc
    12301 ttctggtttt tctggctcga ttttttttag cagcaaatgg atcgcttgat tgtgcaaagc
    12361 gactttcatc cgaacggcat tgtattttgg ttggatctca ttgtattttt ctggagataa
    12421 gacaagcagt ttatagggga tggttttttt taagatgtca gcaagctgga tgatttggct
    12481 atcttttaac tcttttgaat ctctaacacc aagtttacgt aattctgcaa gctgatcacg
    12541 tttcacataa gcagcacata ctgttagcgg accgaaatag ctgccatttc cgacctcatc
    12601 actacctaaa acggataatt gactgatatt ttttggcaaa gaagaggctg tttttgctgg
    12661 cttggcttgt ttatcctgtg cctcccccca tttatcagct tctattgctg cattcgtacc
    12721 ttgaaacatc acttttccgg atgtataagc ggtaatggtc gtcccgtctt tttttgcagc
    12781 aaagctagta taaggaatct gtttatttaa caaataagag cgatagtatg ctttcatttg
    12841 actaagttgg tcgtttgttg ctttgataat gacgttgctc ataaaattct cctcgttttt
    12901 atacataaaa ctataccgca actcacgact tttcgttttc taagagattg cgagacatag
    12961 cattttttta aagtttatca tataatagtt aagtatgtga aaaatatttg tggagaacgg
    13021 tgaaagaacc aacttcatgg tacactaaat aaaactttca tcaaatagga ggaaatgatt
    13081 cgtatggcgc acgaaaaaac acgctacaaa gcggtgattg ccaaccaaac atatactatt
    13141 atcggacggg aaaccaagca tcatatggat atcgtaacga agctgatcaa tgaacagtta
    13201 gcagaattga aacaattatc accacaaatg gataatgaac aagcagcaat cttgatggct
    13261 gtcaatgcgc tttcggatca attgaagaaa caagaaagaa tattggagct ggaagaagaa
    13321 acagcagaat taaagaaaaa aatgatcaaa tttacagagc ttgaaaatcg tgtgaaacgc
    13381 attgaagcaa tcgagaacga agctcgggaa gtattgaaag aaaatggtca agcagatcat
    13441 accattaaaa atcatgtgga agcacaacag attttaaacg agaaacgaaa aggacaaatt
    13501 aaacaaaaat cttctaatta agatcctttt atcaaaataa gaaaaattta aaaaagttag
    13561 aagagaaagg aacgtagcat gctgagtttg ctgattttat ttttattgct aattgcgttt
    13621 ttttctggag cacgtcgcgg gtttgctttg caaatcatat tcgcgattgg atatgtattg
    13681 tcatttattg ctgcccagca tttttacaaa ccattagcaa gtcatttgga attatacatt
    13741 ccatacccag ctgtcacccc aacatcaaag ttagcatttt ttgatcaggt atttgccttt
    13801 cacctagatg aagcttttta tgcaggaact gcttttctga ttattttgct aattggctgg
    13861 ctgcttaccc gatttgtggg ggtttttgtc catggattga cgtatgtgcc gatcttgaga
    13921 caggtagact ggattgcagg aggcatcctg tcactaatca tggcttacgt tactatattt
    13981 ttgattttgc agttacttgc ctttgttcca ttagatgtgg tccaaaatca attcggcgag
    14041 catagtttcg ctcgttttat cgttgagaga acgccatttc ttaccaacaa gatgtatgag
    14101 ctttgggtaa cgaatatttt gaattaagga aaatagtgct ggaaaatatt tcgttttttg
    14161 tacagagtag tttatttaag aaggctgcgt agcgtatgtc cggcacagct cttttttcat
    14221 agtgaaagat aaggtgataa aatgaataac cgaatccttg aaacattgga atttgaaaaa
    14281 gtcaaacaga tggtcagaca atttgtagtg actgcacaag gaaaagaaga actggcagaa
    14341 ttggtaccag tgagtgagaa acaaacgatc acaaactggc ttcaagaaac tgaggatgga
    14401 ttgaaagtgc aacgtctgcg aggcgggatc cctataccta aaattgaaaa tatccgtccc
    14461 catatgaaac ggattgagat cggagcagac ttgaatggag tggagcttgc acaagtttcc
    14521 agagtattgt ctacgacaag cgaattgaat cgtttcattg atgatctttc tgacagtgaa
    14581 atcgaatttg cacgtttgta tatgtgggca gatcagttag tcacgatccc tgttttgagc
    14641 cgccgtttaa aggaagcaat cgatgaagac ggacgcgtga ctgatgacgc ttctcctgag
    14701 ttaaagagca taagacagaa cattcggcga agtgaacaag ctgttcgtga acagttagat
    14761 gggatcgtcc gtgggaaaaa tgcaaagtat ttaagtgatg cgatcatcac catgcgaaat
    14821 gaccgctacg ttatcccagt caaacaagaa taccgcggtg tatttggcgg tgtagtccac
    14881 gatcaaagtg cttcaggtca gactttattt attgaaccaa aacaagtggt ggatttgaat
    14941 aatcgtttgc gtcaatacca aatcgctgaa cgaaatgaga ttcaacgtat tttaagtgaa
    15001 ctttctgctg aacttgttcc ccatcgtcaa gaaatcatcc acaatactta tgtgattggt
    15061 aaaatggatc tgatgaatgc aaaagcacga tttggtaaag aagtaaaagc catcgtgccg
    15121 ggaatcagtg aagacaatca tgtcgtcttg aaacaagcaa gacatccact gatcgatcag
    15181 gaaaaagtag tttctaatga catcacaatc ggaaaagact atcaagccat cgtcattaca
    15241 ggacctaaca caggtgggaa aacgatcacg ttgaagactt taggtctgtt acaactgatg
    15301 gggcaagcag gattaccgat tcctgcaggt gaagaaagtc aaatcggtat ttttgaggaa
    15361 gtatttgcgg atataggaga cgaacagtcg attgagcaaa gtctatcgac cttttcttct
    15421 catatgacga acactgtaga tattttatca aaagtcaatg aaaaaagtct ggtattattt
    15481 gacgagctcg gtgcaggaac agatccgcaa gaaggtgctg ctctagcaat cgcaatctta
    15541 gatgatcttg gtaaaaagtc tgcttatgtg atggcaacaa cgcattatcc tgaattgaaa
    15601 gtttatggat ataatcgggc aaatacgatt aatgccagca tggagtttga tgtggataca
    15661 ttaagtccaa cgtatcgttt gctgattggc gttcctggac gaagcaatgc gtttgaaatc
    15721 tctagccgat taggtcttga tacagaagtt attgatgaag cgaagcagtt gatgaatgat
    15781 gagagccaag atttaaacga aatgatcaca gatttagaga atcgccggaa aatggcagag
    15841 accgaatatc tggaaatgcg tcattttgtt tctgaggcac aagaacttca cgatgatttg
    15901 aaagaagctt acagctattt ctttgaagaa cgtgaaaaag aaatggaaaa agcaaagaaa
    15961 aaagcaaatg aagtcgtgtc agaggcggaa gaaaaagcag aaaaaatcat tgctgatatt
    16021 cgtaaaatgc aacagcagat tgggcaagga aatgtgaaag agcatcagtt gatcgatgca
    16081 aagacccaat tagcaaatct acatcaagaa gaaactttga agaaaaataa agtattgaag
    16141 aaggcgaaag aacaaaaaac gttgaaaccg ggagacgaag tgttggttac gacttacggc
    16201 caaagaggga cgcttttacg taaaaacggg aatcagtggc aagtagaaat cggtatatta
    16261 aaaatgaatg tgtccgaaga cgagctgacg cctgttgctc ctcaaaaaga gccgacacaa
    16321 cgagttatcc atgctgtacg atctgaatct agtagtcacg tgccgaatca attggacctt
    16381 cgaggaaaac gatacgaaga agcgctaagt gaggtagatc aatatttgga ttctgccatt
    16441 ctagctggtt atcctcaagt gactattgtc cacggaaaag gaacaggcgc acttcgcaag
    16501 ggaattactg attatcttaa aaaccatcgg agcgtcaaaa gctttgagtt tgcaccggca
    16561 aaccaaggcg gaaatggtgc gacaattgtt aaatttaagt aaagcttact aaaggtaagc
    16621 agatggttcg ctatccaagt atagaaatgc tatactagtc atagatttta aaaatggagg
    16681 tctttgatat gtcacaagta attacagata agacatttaa tgaagaaaca gataaaggac
    16741 ttgtattgat tgatttttgg gcaacttggt gtggtccatg tcgtatgcaa gcaccgattt
    16801 tggatcagtt ggaacaagaa tatgatgaag aagaattcag aattgctaaa atggatgtgg
    16861 atgaaaatcc agaaactcct caacaattcg gtatcatgag cattccaaca ttaatgttga
    16921 aaaaagatgg tcaagtagta gaaaaagctg ttggtgttca ttcaaaagaa caactacgtc
    16981 aaatgatcga tcaatattta taaaaactta cagcaaaaat aagctgaggg tgggactgaa
    17041 tccagttccg tcctcggttt ttttgcggtt gagttaaggt tattcaaatt aatatttttt
    17101 gagtataatt acgttgtcag gctggattgt ttttttgaca gacatgctat aataacttta
    17161 attgaatatg aaggggagta ccagatgaac gaacgaatca aaaacaaatt agctttactt
    17221 cctgatcagc ccggctgtta cttgatgaaa gataaaaatg gaacgatcat ttatgtcggc
    17281 aaagccaaaa tattaaaaaa tcgtgtccgc tcatatttta ctggtagcca taatacgaaa
    17341 acggaacggc ttgttagtga aatcgaagac tttgaataca tcgttacaga atcgaatatc
    17401 gaagcattgc tcttagagat caatctgata aagaaaaatg atcctaaata caacattatg
    17461 ttaaaggatg ataaaaccta tcctttcctt aagataacga atgaaaaata tccccgtttg
    17521 gtgattactc gtaaggtact aaaagacaaa gcactgtatt ttggaccata tcccgatgtc
    17581 ggtgcggcga atgagacaaa gaagatcctc gatcgattat ttcctctgag aaaatgcaaa
    17641 cctagtcaga cgaaggagcc ttgtctttac tatcatttgg gacaatgcct gtgtccttat
    17701 tatttcgacg tagatccgaa agtctatacg aaaatcgttg atgaagtgaa aaacttttta
    17761 aacggaggac acgaaaaaat cgagtcagag atccatcaga aaatggaaaa agctgctgaa
    17821 aacatggagt ttgaaaaagc tgcagaatac cgagaccaga tccgtgccat tgagacaatc
    17881 atgactcgcc aaaaaatgac aaatactgat ttactggatc gagatgtatt tggttatgcg
    17941 attgataaag gatggatgtg tgtccaagtg tttttcgtaa gacaaggaaa gctaattgaa
    18001 cgagatgttt caatgtttcc tttctataac gaagcagaag aagatttctt gacttacctc
    18061 ggtcagtttt atcaggaaaa tgagcatttc attcctaaag aagtcttgat tcctgatacg
    18121 atcgacaagc ccagtgtaga agctttgtta gctacgaaag tgctgcaacc aaaacgagga
    18181 gaaaagaaaa aattagttga attggcgagt aagaatgcct cagttgcttt gaatgaacgg
    18241 tttgatctga ttgccagaaa acaggaacgg acgatcggag cagttgaacg tttaggaaac
    18301 gcgatgaata tcccgacccc aatacgtatc gagtcttttg ataactctaa tatcatgggg
    18361 acgaatcctg tggctgcgat ggtcgtattc attgatggaa aaccggcaag aaaagaatat
    18421 cggaaatata aaataaaaac agtcaacgga ccggatgatt atgcatcaat gagagaagtc
    18481 atctatcgca gatattcgcg agtgattcgc gaagggttgc ctttacctga tttgatcttg
    18541 atcgatggag gaaaaggaca agtagatgtg gcaaaagatg tcttagaaaa tcaattagga
    18601 atcgatattc ctattgctgg catggtaaaa aacgataaac ataaaacaaa tgagttgcta
    18661 ttcggtacag atttggctgt agtaccgctt gagcgaaatt caccagagtt tttcttactc
    18721 cagcgaatcc aagatgaagt ccatcgattc gcaatcactt tccatcggca gctacgcagt
    18781 aaaaacagtt ttgcttcccg attagatgaa attgaagggt taggaccaaa acgaaaaaaa
    18841 atgctgctta aagagttcaa atcgttgaaa aatatcacag cagccacgac agaagaatta
    18901 caagaaatcg gactgccgaa aaatgtcgca caaaacgtat ttgataaatt acatcaggaa
    18961 tgaggaaagg gcactttaca aagaaagtaa agtgtcctat cattttatgt acaacaaaaa
    19021 gagcgtttac gttttgttgg taaataataa atcgcttacg tctggtgtct aaggatatat
    19081 gtgatttctt gaagaaggag tgaagaaatg ttagaagtca aggatcttgt taaaactttt
    19141 gggaactaca cagctgtcga tcatgtctct ttccaaatcc ccgatggaaa aatcatgggg
    19201 ctaattggcc aaaacggggc tgggaagaca acgacttttc gtttgatctt ggattttttg
    19261 acacaagacc aaggagaagt gttatggaat ggccatcatc ttggggagaa agattatgat
    19321 gtcatcggtt atcttccgga ggaacgggga ctttatccga aagtaacgat tcaagaccaa
    19381 ctgctttatt ttgcagaact tagagggaaa tcaagaaaag agattgaacc aaaaatcgac
    19441 ttttggatgg aaaaatttca ggtaaaaggg aaaaagacgg ataaagtaaa atctttgtct
    19501 aaaggaaatc agcaaaaagt ccagctgatc gccacgttaa tccatgaacc taaattgatc
    19561 attttggatg agccattcag cgggttagat ccggtaaatg ctgaattgct aaaagatgga
    19621 atattggaat tgaaaaaaca tggttcatgt gtcatttttt ctagccacaa tatggataat
    19681 gtagaaaaaa tctgcgatca tttgatcatg ttgcgaaacg gaaaaatggt tttgaatgga
    19741 aaagtacatg agattcgtga atcatttggc cgtacgaagt tattcttgga atctggttta
    19801 agccaacagg aagtagaaga aatcgaaggc gtgaaaaaag tgatccttca tgaagacaat
    19861 acgttagaga ttaccttaga agatcctgaa gctgggaaag aaatctttac tcgtgcgact
    19921 cagttcggct acattcctat gtttaaccag caaccgccaa ctttggaaga gattttcaaa
    19981 ttgaaagcag gtgaaatcga tgcgtaagtt ttggattatt accaaagatg tctatttaaa
    20041 aaatgttaag tcgatttctt tcttgatcat gatattagtc ccttttatca tgctgggtat
    20101 catttatgta gcaggaaact ttgctcaaca aaatagtgat gtcaataaaa tcggcgtatt
    20161 ggctgataat gagcaaatca gtcagcaaat gagccaagtc aaaacagatg attttgagtt
    20221 caaagcagtt gattctgaag aaaaagcaca aaaagagctt tcggaagaaa aaatcgatgc
    20281 gtatttagta gtctcaactg agaatgataa gataaatggg atgctttata gcgaaaattc
    20341 attaggacaa agtacacaac tactgataca acaacaactg actggcttgc aatcgatgct
    20401 tcgagcgagt caattaggca tttcaccaga agaagtagct agcttgagcc aacaagcaag
    20461 tttgactcga caaaaagtca attttgattc aaaaggaaaa atgacagtcg gagaagataa
    20521 ctcgacagtc caatacgtcg taagttatgt agcaacgatt gtcttgttca ttattatttt
    20581 gacgtatgcg cagatcattg cacaagagat cgcttctgaa aaaggcacgc ggatcatgga
    20641 agtgatcctg tccagtacga ctgcacaaaa gcatttttac ggaaaattaa caggtgtttt
    20701 acttgttgca ttaacgcaaa tggcattata cggtattatt tttggtatag gatacagtca
    20761 attcaaagat atggaaatgg tcaaaaatat attagacggt atctcattgg ataacttgat
    20821 tggaccattc ttgtggtatt ctcttctatt catgatcttt ggcattttga ttttttctgt
    20881 tttagcagct ttatgtggct ctttggtcaa caaagcagaa gatacagcaa aagcaatttt
    20941 gccagtgact tatctatctc taggcggata catgcttgga ttgatactag gagcttcaga
    21001 tccaaacaat atcgttattc ggatcacttc gtatattcca ttcttatctt catatattat
    21061 gccgattcgc ttagcaaatg agacagtcgg tatgggtgga gcattgattt ccttgttggt
    21121 acttgtagcc gtaacattct tattgatgat cttgtctgca aacatgtaca aatcgaatgt
    21181 actcgtttat agcgaaggcg gattatggag ttcattgaaa caatcgattt caattatgag
    21241 gaatgaacga aaaaaagcat aaaacaatcc taaaatacag aaggaagctg gacatctatt
    21301 gttcccagct tctttttgta tagacctatt ttatttattt tactatattg gatatatatt
    21361 cattgaaaat agtgtataac aactttataa tattgagtat accaattttt ggaggcgttt
    21421 taatggaccg ttatttagat ttactttcag aaaaattccc ctcaacggag gatgtcatca
    21481 cggaaatcat caatctcgaa gcaattttgc agcttccaaa aggaacagag ttatttttga
    21541 gtgatataca tggcgaattt cctgcttttg atcatattct gcgtatcggt tcaggaaatc
    21601 ttaaagaaaa gatcaaagac cttttttctg aacaaatgac ggaaacggat atcagccaat
    21661 tcactatttt tacggcttat ccagaatatg cattaacgac tgattggtac aaagaacaag
    21721 ataaatcgca gctgatccat catttgatcg atcttttacg gtttacgaca gtcaaatata
    21781 cccggtcaaa agttcgaaaa ggattgccga aagaatatag ctatatcatt gaggaactct
    21841 tgtatatcga tgaccgtatc aatggaaaaa aagactatgt aaaaaaaatc attgaacaac
    21901 ttgttttgat gaaggaagaa gaaaaatttt taacgaaact tgcacagacg atccaaaaat
    21961 ctgtcatcga tcatttgcat atcgttggag atatttttga ccgaggaact caagcagcca
    22021 aagtgatgga tcgaatcatg gatttttctt ctgtagatat ccaatgggga aatcatgata
    22081 ttttgtggat tggtgcgtat tgcggctctg aggcatgttt actgacactt ttacggattg
    22141 cagcacgcta taattattta tttgaattag agaaagaata tgggttgaat ttacgtccat
    22201 tgttttcttt tgctcatcaa acttatcaga agaatccagt atttgcgcct aaaaacagta
    22261 aaaatctatc cgaaagagag caagaagaat tagaaaaaat ccatcaggca ctttctatcc
    22321 ttcaattcaa atcagaacat cagttattgg atcgtcgacc tgaatttaaa atggacgatc
    22381 gtaaattgct ggaaaaaatc gactatgaag catcaacgat tgatttagaa ggtcagctct
    22441 atgagttgaa aggaacttgt tttcaaacga tccagccaga tgatccaaaa aaattcttgg
    22501 aagaggaaca aaaagtgatt gatagtttga tgtattcatt ccagcactct ttgagaatgc
    22561 aaaaacatat gacgtttttg atcgaaaaag ggggaatgta tctcacgaac aatcagcatg
    22621 tgctgtttca tggctgtata cctgtagacg agaagggaca gctcttagca tttgaattag
    22681 accaatctta tcgcggaaaa gaactgctgg gcttttttga aaaacagata actgaaagcg
    22741 caaaagatct gactgcaaaa gaagatttag caaccgattt gatttggtat gcgtggagtg
    22801 ggccattttc gcctttattc ggcaagagta aaatggcgac ttttgaacgt tattttctca
    22861 cagagcctca tacgcacgtc gagaaaagca atccttatta tcacttgcga gatgaagagt
    22921 ggttcagtga aaaaatttta gcagaattcg acacaaaaga agaagggtca gcaatcatta
    22981 atgggcacac gccagtaaaa gtaaaaaaag gggagtcacc gatcaaagca aatggaaaag
    23041 tatttgtcat tgacggaggt ttatccaaag cttatcaaaa aacgacagga attgccggtt
    23101 attcactatt atgcaattct tatggtttcc aaatcgtgac acattatccg tttttgccta
    23161 ttgaacaaca atttgcaaaa aaatccgacc aaacaaatgt gaaaaaagta atcgaacagc
    23221 aactaccacg caaactcaat cgtgatacaa caaaaggtat ggaactgcag cagcagatta
    23281 cccagctcaa aaggctgttg gactatcgaa aggacgattg agaagaattc cagtgaacat
    23341 tgttccatag actggagact gagacaaaag tcgtgtcgca gtctcttttt ccggataaac
    23401 ggtgttcttc tgccaactcc tcggtcacaa gtcacactat tcaacaaaca tgaaaagctg
    23461 ttttttgaaa ttgctccttg tgactcagag ttgaacggca ggctcacgac ctcttggtat
    23521 aaacggtgtt caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtgaaaa
    23581 gccattttca caaaattttt cttaatactc gaaactaaac agctttttca caaccccctc
    23641 agaaagtcta ttaaaattca ttcacaaaga aatcgaataa gcgctgttca gatggatgta
    23701 cacggtgggt aagttccgga tgccattgaa caccgaggat tttatgcata tcatctttgc
    23761 tttgaaccgc ttcaacgagt ccatcgttag aaaaagcgat ggcttttaat aaaggtgaaa
    23821 gatctttgac tgcctgatga tggtaagaat tcacaaaata tggatcgggg agaaggttgc
    23881 ttaacaaact gtctgaaaca atcttgactt cgtgagtcgt aaattgcggg gcagtgggct
    23941 gttgttcatg ttttactgac cactttggat ataatgaaag atcttgataa agtgtacctt
    24001 ccaaaacgac attcaatagt tggagtcccc ggcagactgc aaaaattggt tttctttgtt
    24061 tcaatgcttc caagatcagt gcctgttcaa attggtctcg ttgaatattt gtttccgtta
    24121 gctttggatg aggatcttcc atgtaaagtt gtggactgac atcttgacca cctgcaagaa
    24181 taagtttgtc gatttgtcca atcagttgag gagctgaatc tggagacatg actggcagaa
    24241 taagaggcaa tccttgagca tctaaaactg catccacgaa tccttgcggt gtatagctca
    24301 cttgatttcc ttggaaagta tctgttgctc gaatcagttg atttccggca atgccgatga
    24361 ttggtttcat ttgtattccc cctaaagtag acttattgta acaaggattt aaaaaaatta
    24421 aactatgtaa gacttgcccc tatacttatc tgttcttgct gtgttactat ctgtaaagag
    24481 aaatttcgtg tgaaattcat gacgatagtt gaaaatattg cttaatttgt tataataaaa
    24541 agtaagcaga aataaaaagg ttgggcgtaa gcttcaagga tctagttctt caaggggtga
    24601 gaaggacatt ttattgaagg ggcctgccta tactaagtta tctttagagg tacatacttt
    24661 caatgaaaaa agaaagcatg tacctctttt ttaaatcgaa aggaagtgaa gcaagtgtta
    24721 gaagctttgg aacaaatccg aaaagcagaa gaagaaaatg aaaagcgtgc tcaaacactg
    24781 cggcagcagc tgaagatcta tgcaacagaa aaagagcagg aattggaaac gatccaaaca
    24841 aactatcgac aacaactaaa acagttggtg gaagaaaaag aacaatttga aatacaagaa
    24901 ttagaaaaag aaaaaatgaa attgaaagca gaagcagaca aggtgcaagc aactttgaaa
    24961 gatcaatatc aaaagcatca gagtcaagcg atcggtgcga tcattgaaag ggtgaaagaa
    25021 acttatggcc gtcactaaga tggaaaaagt gaccctcatt tcagataaaa gaaatcagga
    25081 gaggatttta caagcagtac aaggaatcca aaacgttgaa atccgggatc tgtatcaaaa
    25141 tagaacaaac aaccagtggg tggagaagta ttttccagat accacgatta ttgataaaga
    25201 agcaaaatta agcgctttag gtaatcggtt gaaagacatc caagaagcta tccaattcat
    25261 acaacaccac ggcgatagcc gtcaaaagaa actccatttg aaacgtcaag aactttcctt
    25321 acaagatttg gaagccaatt attccgaaga aaatttcata aataaattga aagaagtcct
    25381 tgatttaaaa gaaaagtggg aacaactaac tgaagaacgt gatcgtttag aagaagagga
    25441 agaatggctt cttcattggc aacagctgga tattgctcca agtatgtatg caagtaagac
    25501 gaccattttt gagatgggga ctgtgagtgc agcaaatttt gaaagtttca aggaagaact
    25561 gagtaagctt gatgacgttt atatggaaga aatagattat acgccaaaac aagtttatct
    25621 ttcgtatgtt gctttaaaaa aatcagttgc tgttatagaa gaaattgctg ggaaatacgg
    25681 gttcaataaa gaaacgtatc cttatgaaca aaatcctaaa gaaaaactgc aagagaccaa
    25741 gcagcaatta tccattgttc acgagcagca aaagaagata acgacagcgt taggtcagtg
    25801 cagtggctat atccaagaat ttgaatgggt agaagaagtc acacttgccg ttgcagaacg
    25861 agaaaaaata aaagaacggt ttatccatgc gacttatctg atcgttttgc aaggttgggt
    25921 agatgcagaa gaaaaacaga atttgctgca tgtgttaaac gaaaccgtat cagaagaaga
    25981 tatatacgtc agtttcgaaa caccaaactc agaagagatt cagcaggaag tccctactaa
    26041 attgaaaaac catccgcttg tcgaaccttt tgaattgctg actgaaatgt acagtctgcc
    26101 aaaatatgaa gaagtagatc caacaccttg gatgacacca ttttatcttg tcttcttcgg
    26161 gatgatggtc gcagatgttg gttatggttt gctgatgctc attgggacaa tactagctca
    26221 aaaactactt gttctaccac gcgggatgaa acgtttcgta aaattctttg agattctctc
    26281 gataccatcg atcatctggg gattgatcta cagttcattt ttcggacagt cattaccaaa
    26341 agaactgttt ggcatccgct tgcctttccc aatcctatct acgacggatg acgtcaatac
    26401 gattttgatt ttatccgtga ttttcggtct gatacagatt ttggttggat tatttgtggc
    26461 agcaaaggaa aatatcagga gaaaagatta cttgagtgct gtcagtgatg gatttgcctg
    26521 gcaagggatc ctaattggga tcgttattgc gttggttggt gccatgttga taaaaaatac
    26581 agcatttgtt taccttggcg ctgggctatc gatcattgct gccttatgta tcttgatcgt
    26641 tccgattatc caaacgccgt ctaaagtcaa aggtgctgcc aaaggtgcgt ataatcttta
    26701 cggactgacc ggctatatcg gtgatttagt cagttataca cgtttgatgg cattgggtat
    26761 ttcaggcgga agtattggag cggcattcaa tatgctagtt gctttcatgc cgccagctgc
    26821 tcgattcagt gtagggatcc ttttgatcat cgctttacac gcactgaata tgtttttgac
    26881 actgctaagt gcctatgtcc atggcgcacg attgcagtat gtcgaatttt ttgggaagtt
    26941 ttatacagga ggcggacgtg cctttgatcc gctgaaaacg gcggaaaaat acgtcaacat
    27001 caatcacaag aaaaaaaaca ataaataatt ggaggatttt tatataatga tggattactt
    27061 aattagtcaa aatggtggaa tggtatttgc ggtattagcg atggcaacag cgacgatttt
    27121 ttcagggatc ggttcagcta aaggtgttgg aatgacaggt gaggcagcag cagcattgac
    27181 aacaagtcaa cccgaaaaat tcgggcaggc tctgattctg caattgcttc caggcacgca
    27241 aggtttgtat ggattcgtta ttgccttttt gatttttatc aacttaggaa atgacatgtc
    27301 agttgtacaa ggactgaact ttctaggtgc ttctctgccg attgctttta caggcttgtt
    27361 ttcaggtatt gcccaaggaa aagtcgcagc ggcaggtatt caaattttag cgaaaaaacc
    27421 tgaacatgct acaaaaggaa tcatttttgc tgcgatggtt gaaacatacg cgattttagg
    27481 tttcgttatt tccttcctat tagtgttgaa tgcctaagca taaaggagga aaagcagatg
    27541 aacgccattg aaaaaatcat ctctcagatg aacgaagcag cagagcagga aagagcggcg
    27601 ttagaacaag aagagcgaat gaagatcgat caaaactttg aacaaaaacg tacgcaagtt
    27661 gaaacagaac atcaaaaaca aaaagaaaaa caaatcgaat tactcgaaaa aaaataccgt
    27721 cagctaagga atcgccagca agtagaagta cgacaagaaa atttgaatgc gaagcaggaa
    27781 tttttaagac gattgtttgc agatgcggta actgaaatgg aaaattggga tgaatctgaa
    27841 caaatccagt ttatcaaaaa tgcattgtat tcattacctt tgacaggaaa agtagcattc
    27901 attgctggag aaaaatcagc agcctatctt tctcagacgt tgttggatga atggaacaat
    27961 gaacttccct ttatgatggt tttaagtgat gaaactgttg ctgatcaagc gggttttctg
    28021 ataaacgatc aaggagttca gtataatttt cttttcagca gccttgtaca agatatccaa
    28081 ggaacgatga gttttgagat tgccaatcaa ttattcgaat aaggagggat cagtgtatgg
    28141 aataccacga actaaatccc ttgattcgcg gaagagagct tgaacttttg tccaatgaga
    28201 catttgaacg gatgattcaa acaaattctg tcgaagcact tggtgaaata ttgaaatcca
    28261 cgatttacag tccatatatt tatgacggat atgaatatga cttcgaacag aatcttttaa
    28321 aggagcagag ccgtctattt cgctggttaa aggaatcagc accggagcct gaaatcgttt
    28381 ggatctatac gatgcgttat actttccata atctgaaggt tttgacaaaa gctgagatga
    28441 caaatcaaaa tctggaccat ttgtatatcg acgatggatt ttattcatta gaaaccttga
    28501 aggatgccat tcatacgcaa gtttcaaatg aacttcctga aagcattatg gacagtatac
    28561 gagaagtacg tgattatttt gaagagtcta ccattcttca gggaattgac gtgatttatg
    28621 atcgacagtt tttgacagag cagcgacggc ttgccgataa gctaggatat caagaattgc
    28681 tgacagaagt cattgctttt attgacttga ccaatatcac aacgatggct cgaggaatat
    28741 tgcagaaaag aacatatggg ttcatgacga cggtactttc cagctcggga agtatcgaaa
    28801 aagaagtatt tttgaacttt gtagataaag atatggaaga ttacacgaat tttctattag
    28861 agacagatta tgctgatttt ctgcgccctg tcatccgtga gggaaatatt gatctcgtaa
    28921 agcttgaaca gttgaaagat gattatctaa gttcattgta tcaagaagca caaacccagg
    28981 ctttcggacc actgccacta ctagcctttt tgaacgcaaa agaggtagaa agcaaaaacc
    29041 ttcgtttact ggttatcggc aaaagaagcc atttcgaatc agaaacaatc agaaagcggg
    29101 tgagacaagt ctatgacgca taagatcggt gtagttggcg acaaagattc tgttttgcca
    29161 ttcaaattgt ttggctttga tgttcgttat ggtacaacca agcaaaccgt tcgtcaggca
    29221 attgaagaaa tggcaaagaa tgagtatggc gtgatttatg tcacagagca atgtgcagca
    29281 ttgagcccag aaacgatcga acgttacaag gaacaaatga tacctgctat cgtattgatt
    29341 cccaatcatc aaggatcatt agggataggg ctggaagaaa ttcaaaaaaa cgtagaaaaa
    29401 gcagtaggtc aaaatatctt atagtatgaa aaggagatag aaattttgca aattggaaaa
    29461 atcataaaag tctccggacc tcttgtaatg gctgagaaca tgtctgatgc cagtatccaa
    29521 gacatgtgtc tagtgggaga tctaggagtt atcggagaaa ttatcgagat gcgtggcgat
    29581 gtggcgtcta ttcaagtata tgaagagact tctggaattg gtccaggcga accggttcgc
    29641 tctactggtg aagctttatc tgttgaatta ggaccaggga tcatttcaca aatgtttgac
    29701 ggaatccaac ggccattaga tacctttatg gaaatcacac aaagtaattt cttagggcgc
    29761 ggagttcaat tgcccgcatt ggaccatgag aaaaaatggt ggttcgaacc aacagtagaa
    29821 gccggagaaa cggtgagtgc tggagatgtt atcgggatcg tagaagaaac aaaagttata
    29881 aaacataaaa tcatggtgcc aaatgggata aaaggaacga tcaaaacaat cgaaaaaggt
    29941 tcattcacta ttgatgaaac agtatgtgtc attgaaaccg aaaatggtga caaaaatttg
    30001 acgatgcttc aaaaatggcc agtaagacgt agtcggccaa tcaaagaaaa attaaacccg
    30061 gatgcaccca tgatcactgg acagcgagtg attgatacat tcttcccagt gacaaaagga
    30121 ggagcagcag ctgtacctgg tccttttggt gcagggaaaa cagtcgttca gcatcaaatt
    30181 gcaaaatggg ccgatgtgga catggttgtc tatgtgggat gtggcgagcg tggaaatgag
    30241 atgacggatg ttttaaatga attccctgaa ttgattgacc ctaatacagg agaatcgttg
    30301 atggaacgta cggtattgat tgctaataca tcaaacatgc cagtagcagc tcgagaagct
    30361 tctatctata cgggaatcac aattgcggaa tatttccgtg acatgggtta tgacgttgct
    30421 atcatggcag actccacttc acgatgggca gaagcacttc gtgagatgag cggacgtttg
    30481 gaagaaatgc ctggtgatga aggttatcca gcttatcttg gttcacggct tgcggaatac
    30541 tatgaacgat ctggaagagt cattgcatta ggatcagaag gtcgcgaagg aagtataacc
    30601 gcaatcagtg ctgtatcccc gtctggaggg gatatttcgg aaccagtcac acaaaataca
    30661 ttgcgggtcg taaaagtatt ctggggtctt gattccagtt tagcccaaaa aagacatttt
    30721 ccttcgatcg attggataca aagctattct ctttattcaa cagaagtagg gaagtatatg
    30781 gatcatatcc ttcaagctga ctgggcatcc atggtcaccg agggcatgcg tattttgcag
    30841 gaagaacagc agctgaatga aatcgtgcgt ttggtaggga ttgattccct ttcagataat
    30901 gaccgtctga cattagaagt ggcaaaatcc attcgtgaag attatttgca gcaaaatgct
    30961 tttgatgatg tcgatacatt cacttcaaga gagaaacaat tcaaaatgtt gaagatgatc
    31021 ttgacctttg gtgaagaagg acgccgtgcg ttgtcgttag gtgcttattt aagtgagatc
    31081 atggacggta ccgtatcgat tcgtgaaagg atcggacgaa gcaaatatat cccggaaaca
    31141 gaattagaaa aactagatca aattattggt gaaatcaagc aaacgatgca aacgatcgtt
    31201 tcagaaggag gcatgagcaa tgattaaaga atatcgtacg atcggtgaag tcgtcggtcc
    31261 attgatggcg gttgaaaaag ttgctggtgt caaatatgaa gaattaatcg aagtccgtat
    31321 gcaaaatggc gagattcgtc gtggacaagt attggaagtc caagaagata aagcaatggt
    31381 ccaaatattc gaaggaacaa gtggtatcaa tttacgtgac tcttctgtac gatttttagg
    31441 ccatccttta gaactaggtg tatcggaaga catgatcggt cgagtattcg atggtttggg
    31501 acgaccgaaa gataacggac cagagatcct gcctgaaaag catttggata ttaacggaga
    31561 agtaatcaat ccagtagctc gggattatcc agatgagttt atccaaacag gtatctctgc
    31621 aatcgatcat ttgaatacat tagttcgtgg gcaaaaatta ccagtatttt ctggctctgg
    31681 tcttcctcat aaagaattag ctgctcagat cgcgcgacaa gcaacggtac tcgattcatc
    31741 agatgatttc gccgtagtct ttgctgctat tggaatcact tttgaagaag ctgaattttt
    31801 catggaagat tttcgtcaaa cgggcgcaat cgaccgttct gtcatgttta tgaatttagc
    31861 aaacgaccct gcaatcgaac gtatcgctac tcctcgtatg gctttgactg ctgcagaata
    31921 tcttgcttat gaaaagggaa tgcatgtttt ggttatcatg acagacatga ccaactatgc
    31981 agaggcattg cgagagattt ctgcagcacg gcgagaagtt ccaggaagaa gaggatatcc
    32041 tgggtatctt tacacgaact tagctacttt attcgaacga gcaggaagaa tccgcggact
    32101 aaaaggatcg gttactcaaa ttccgattct aacaatgcca gaagacgata aaactcatcc
    32161 aatccctgac ttgactggtt atattacaga aggacagatc atcttgacta gagagcttta
    32221 caagagtggg attcagccac caattgatgt actcccgtct ctttcgcggt taaaagataa
    32281 gggaactgga cccggaaaga caagagaaga ccatgcagct acgatgaatc agctttttgc
    32341 agcctatgca caaggaaaac aggcaaaaga attagccgta gttttaggtg aatctgcttt
    32401 atctgatgta gacaagatct atgcgaagtt tgctgagcgg tttgaaaaag aatatgtcaa
    32461 tcaaggattc tacacgaacc gttcgattac tgaaacactt gatctagggt gggaactgct
    32521 atcgatgttg ccaagaaccg aattaaaacg aatcaaagac gacatgctgg acaaatatct
    32581 gccagaaggg aagtgagttc tgatggcacg attgaatgtg aatccgaccc gcatggaatt
    32641 gactcgattg aaaaaacagt tgacaactgc cacaagaggg cataaattat taaaagataa
    32701 gcaagatgaa ctgatgcgtc aattcatcct gttgattcga aagaacaacc agctgcgtca
    32761 agaaatggaa gagtctatga cagaagcaat gtctgatttt gtattggcaa atgcttcgat
    32821 cacggaacct ttcatcgaag aattatttgt tttgccggcg gaaaacgttg atttgtctat
    32881 cgtagacaaa aatatcatga gtgtaaaagt acctgtgatg aattttgaat atgatgaaca
    32941 gctgacagac acacctttgg aatacgggta tttgaattcc agtgccgaac tggatcggtc
    33001 aatcgatcgg tttactaccc tgcttccaaa acttttggaa ctgacagaaa tcgaaaaaac
    33061 ttgtcaactg atggcgagtg aaatcgaaaa gacacgtcgg cgagtcaatg cattagaata
    33121 tatgacgatc cctcaattag aagaaacgat ctattatatt cgtatgaagc tggaagaaaa
    33181 cgagcgcgca gaagtaacaa ggttgatcaa agtaaaaaat atgggtacca ctgactaagt
    33241 ggtatctttt tttaaaagag gaaaacgatg attaaacaaa gcaaacaaat aaagaaacgt
    33301 ttgccggctg ttcagcttct cgcattagga ttttttctcc tgattttagt tggcggcggc
    33361 ctacttaccc taccattttt tagtaatagt gggcatggga caaattttat cgatgcgctt
    33421 ttcactgcaa cttctgctgt ctgtgtaacg ggattgacaa cattgaatac agcagagcat
    33481 tggaacagtg ctggtcaatt tttgatcatg cttttgattg agatcggcgg tcttggtttt
    33541 atgatggttc cgattctatg ctttgcattg ataaagaaga aagtcagtct cagcacacgt
    33601 attatattaa aagaagcgct gaatctagaa gaagtatcag gtgtgatgaa gctcatgctt
    33661 tacatcctaa aattggcctt tgtgatccaa ttgattggta cggttgcttt atcttttgta
    33721 tttgtgcctg aatttggctg gttgaaaggt atctggtaca gtttgttcca tgcaatctct
    33781 agtttttgta atgcagggtt tgatttgtta ggagacagtt tagccaatca ccagcaaaat
    33841 gtttatttga tcatggtggt atctgctttg atcatttctg gtgggttagg cttcatcgta
    33901 tggcgtgatt tactggaata tcatcagaaa aagaagatga cgattcattc aaaaatcgca
    33961 ttaaccgtta caggtaccct tttattaggc ggattcgttg tgttcttttt cacagagcgc
    34021 aatggggcaa cgctagtgga agggaatatg tttcaacgat tggcaaacac cttttttatg
    34081 agtgtgacac ctagaacggc tggatattat tcagttgatt acctccatat gagccatgca
    34141 ggtctgattt taacgatgtt tttgatgtac atcggcggaa catctgggtc gacggccggc
    34201 ggattgaaaa caactacgtt aggtgtgttg ctgatccaga tgcgttcgat gttgaaagga
    34261 cgtacgagag cggaagcatt tggccgtacc atccgacaag gcgcagtgtt gcgggcactt
    34321 actttatttt ttgttacgtt gacgttgtgt gtagtcgcca tcatgatttt atcggtgact
    34381 gaaacgatac ctaaaacttc tggcatcgaa tatattgctt ttgaggtctt ctctgcgttt
    34441 ggtacagtag gtcttacgat gggattgacg ccagacttga ctgcaatagg gaaattgatc
    34501 attatagcgc ttatgtacat tggacgagtt ggtgtattga ctgtggtctt ctctctgatg
    34561 acaaaagcac aaagaacaca atcaaaatac aaatatccag aagaaagtgt catgattgga
    34621 taatttcatc ttctgacaga aacctagtga tttagtaaaa taagaaaata aaataatttt
    34681 tcttgcttct tggatacttt aagtgcaaaa tgttcgttta ttttttgagg cactacccgt
    34741 tgttgaacct cgggtagtgc ctctttttta ttgaaaggaa tcaataagat ttgattcctt
    34801 tatttatcaa taggttataa ttcatatttt tttatttttt cttttacgat acttagtcca
    34861 gaagctaaaa tttcaaggtt attagaggat agggaaattc gaacgaatgc atctggttgc
    34921 gcacgtgtag taaagcgtct ggaatgataa agtcggatac cgttagcaag aaagtcattc
    34981 tcaattatct tagggtcaat ctcaggatca actggaagaa ttcgatagta agggacacga
    35041 ttcgttggtt tttctacttt gaaaagagaa tcaaaaagct gattggctct ttttgtaaga
    35101 cttaatttag tttctaagat cctatttgct tcatttgaat ggaggatttg agtgatgact
    35161 tccacatcta atccagaagt tttcacattt atattaaaaa ttgcttgttc tatcttgctt
    35221 tggacatgta ctggaactac aagataagca actcgcaatc cagaacaaat aaattttgtc
    35281 attccagcta agtagattgt ttggttaggt aataaatttt ggaaaggagg aatgatcgta
    35341 tgttgatagt aagttgttaa aaatgaatgg atatcatctt caataaccca tagaccttct
    35401 ttttcaatca cttcggctaa ttgctctcgt cttttgtgag agatttgaaa ccctatagga
    35461 ttattacaag aaggcataag aaagacacca tgaatcttct tttttctaca ttcctgaatc
    35521 aatatatcag gtcgcattcc ttgctcgtca aaatcaattg gaacgatctc taaatgatat
    35581 aactgagcca attcaatgaa attagcgtat gtatatcggt ctgttgctat tctatcacct
    35641 ggagagaaaa gtgcagaaag tgtgatagcc aatccatttt gaacaccgga aacaatagat
    35701 agagattcta aggacggaat gactccttga gtttttaacc aatttgctgc agtagtcaat
    35761 tgaaaatttg tgcctttggg atcttggtaa ttaagcaaac caacaagatt tgggttatta
    35821 ctagttgatt gaataaacgg gacaacgaat ttattacatt cttcaaaaga actaaccaga
    35881 ccaaaatcaa tgatatgttc ggttacttca ttggtagaga tggtgatgga ctcaaagaca
    35941 ttgggagaaa taaatgtgcc acttccaata ttcgtataca gcaaaccttt ctcaattcca
    36001 tatttataag ccttgccaac ggtagtaaaa tttaaatcta aataatcagc tagctctctt
    36061 tgggagggta actgagtgtt tttctgtaat ttattcttta aaatatcttg ttctaattgt
    36121 tgaatcaacg atcgataata aggacgcttc aatttttgtt tgtctggtcg ccaagttaac
    36181 gggtaattgt cataagaatt aataggcatt ttctccactc cttaaaattg tataccatac
    36241 aattttacca ttgattgtat tgattttaga agttaatctt ttgtgaggag ggaattgtct
    36301 atgaaaatta ttaattttga ggaagtagta gcttctttta gttttgaaga agcgattcaa
    36361 gtaatgaaaa aatgtttttt tgattatgaa agtggtaaaa tcagccaaaa tcctcgattg
    36421 gtggaaattt tacctgacgg aaaaggcaaa aatatgtttg cgatgatgcc tgcttatttg
    36481 ggggagaaca gatattttgg cacaaaagtc attaccgctt ttccggataa tcatcagtat
    36541 catttacctt ctcatttagg agagattttc ttgtttgatg ctcaaaacgg actacccaaa
    36601 gcattgataa acgcaaatgc agtcacctgg attcgaacag ctgctgtatc tgcgctggca
    36661 acagattact tagcaaatcc gcatgcagaa acacttgctt taattggagc tggtcaacaa
    36721 gcattgtcgc atttgactgc gatttctacc attcgtccca tcactacagt gtttgtttac
    36781 gatattttag aagaaagacg aaatactttt atcgctaaag ctaaaaaaca gtatcctaaa
    36841 ataacgttta taaatgcaat atcagtagaa gatgcagtac gtccagcgga aatcatttgt
    36901 acgttgacct ctagtaaaga ggcatttctt caaaaagagt gggtaaggga agacgcacat
    36961 atcaatgctg tgggcacatt tactcctgat acaagagaga taagtacatc gttgatggaa
    37021 acttctcaaa tctatgtaga tgattactca gctgccttaa cagaaagcgg ggacctgctt
    37081 atagccatgg cagaaggaga tttagaggaa tcttgtattg ttggtacatt aggcgaatta
    37141 gtttctgaga aaaaaacatt ttataaagga gaatcgaggc cgacgatttt cgacgcagtt
    37201 ggtctagcta ttgaagatct ttgttgtgca gaatatattt ataacaaatt agagaaaggg
    37261 gctttaaaat gaacttagat tattacgtag ttgatgcatt tgcagatgag gtgttcaaag
    37321 gaaatccagc agcggtttat gtgctagaag aatggctgcc agaaggtacg atgcaaaaaa
    37381 ttgccattga aaacaattta tctgaaacag catttacagt gaagaaaaac caagaatttg
    37441 agcttagatg gtttacgcca gatcgagaaa tcgatctttg tggtcacgcg acattagcaa
    37501 cagcatttgt tttatttaac tattacaaaa tacctgatga aacgattaaa ttttctactc
    37561 aaagcggtaa tctatttgtg acaagaatta aagattatta ttacatggat tttcctagta
    37621 ttatgccgaa aaaagtgccg atattgtctg aatatgaaga agcgattggg gctaagatta
    37681 aagaagccta cttagcacgt gatttatttt tcgtattaga agatgaaaaa acggtggcta
    37741 aattgcagcc agattttaca gctatcaaaa attttgaatt aggaatagga gtcatcgtga
    37801 cagcagaaag tgtgcaaaaa gactttgttt ctagaacctt ctttccaaaa ttaacaatca
    37861 atgaagatcc agtatgtgga tcagcacatt ctaatcttat tccttattgg gcagaaagaa
    37921 tgaataaaac aaaattgtca gcttatcaaa tgtcatcaag gggcggggat ctagaatgtg
    37981 aattaaaaga agatcgtgtg ataataggag gaaaagcagt tttgttttct aaaggtacct
    38041 cgtttatttg atttctttca aatgaattaa aaataaattg gtggagagtg gcaaccttat
    38101 agctacaatg gttgttaaaa aaagacgcaa actttttcaa tttaatgaaa agtttgcgtc
    38161 tcattttaat tattggtgtt tgttttttat tcaatcaaga gtaaaatgcc ccatactaac
    38221 tatcctcgga tgacttcgat tttataacca tcagggtcaa tgacaaagta gtaagaaggc
    38281 gcagtgccag gtaatccttt taagtcagta atctcaagac cagcagcttt atgttgctcg
    38341 tgtaattttt ctagatcatc agctgctata gcgatatgac catagccgtc tcctagatca
    38401 tacgcaccat gatcatagtt gtaagtcaac tctaattcat agtcatcccc aggaagtgtc
    38461 aaataaacaa gtgtaaattt gttttctgga aaatcacggc ggcggctttc ttcaaaacca
    38521 aatgcctttt gataaaattc gattgatgct tcaagatctt tcactcgaac acatgtgtga
    38581 gccattttca tagattcatc tctccttttc cttttactca atcataacaa aaagaagtaa
    38641 ggaaatagtt gatttgtgct tgttcatgag aaatttctca tgtaaaatgg atagaaaata
    38701 aaactgttaa ggtggaaact attcatggat aaaccaattt ttggtgaaaa aaagcaattt
    38761 gctgattatc aaacccgtta tgcagcatat attgtcatag aaagagatca gcagattgca
    38821 gtaatcgagg ctccaaatgg tgcttttttt cttcctggcg gggaaattga aggagaagag
    38881 acgaaagaag aagcgattgt ccgtgagctg atagaagaaa tgggaattgt ggcagaaatt
    38941 tcttcttatc tgggagaagc tgatgagtat ttttactcga attatcgtgc aacttattat
    39001 tataatccag gatatttctt tgttgctgat tcttggaagc gtataggaga accaactgaa
    39061 aagaccaata aaatttggtg ggtgagccct caagaagcgc ttgtgaaatt gaaacgtggt
    39121 agccaccgat gggcagtcga gagatggatc gaaaaaaata aaaactgaaa gaaagcgagt
    39181 agatcagctt tctctcagtt tttttattta ttacagtaca tattttttga ttgcttcagc
    39241 tacaccgtgc tcgtcgtttg atgaagtgat gacatttgct gcttctttga caagagaaac
    39301 ggcatttccc attgctactc ctagaccagc atattcaatc atggataagt cgttctcgtt
    39361 atctccgata gccatgattt catcttgatc aataccaagg tgctgtgcga gattcgcaac
    39421 tgccgcacct ttacttgctt ctttatttag tacttcgaag taatagggcg cacttttgac
    39481 cgttgtgtat ttatcacgga aagtttgcgg taaacgcgca attgctgcat ctaaaatttc
    39541 tggttcgtcg atcatcatca ttttgacgat gttcatgtct ggtgtcattt cttcaggtgt
    39601 tcgatatttt agcggcatct tgactaaact tgcttcgtgg atcgtataag cgctgatatc
    39661 tcgattaggc gtgtagatcg ctttgtcatc gatcgaatgg agatgactgc cgacacggcg
    39721 tgccattact tcgatctcta ggaaatcttc atgtgtcagt ccgtgacggg cgatgatttt
    39781 acctgatttc gtttgttgta cgagtgcccc gttataagta ataacgtaat cattatcttg
    39841 aaaaaggttc aactcgttta actgttcctg gacacctggt aagggacggc cagtgcataa
    39901 aacgatcgaa acaccttttg caacagcagc ggcaatcgct tcttttactt cttttgttac
    39961 ttctcttttc tcatttaata aagtaccatc gatgtcgata gcgactagtt tgatagacat
    40021 aatttcctcc aatatatttt aatttatttc aatcaatgcg ccattacgga tatggctggc
    40081 aaattcttga taagtttcgt caaataaatc ataatgatca cgcaaggacg gatcaaccat
    40141 ttcttttgga aagaaaaatc gctcatctcc ttgttcttgt cccgctaatg cagcaacaag
    40201 ctgactaacc tgtgataatt ctatcaaagt tccgtcattt cgcaaaatct cgatttgtgt
    40261 gcgatgtctg ccttgttctg ggcgataaaa atcataaggt agatcgtagc tagaattcac
    40321 agcagtgtaa taaacaggat tatatccgac cttttctact aacaaagtga gttcttggac
    40381 taattttgaa tcccggttgc cagaaaaagt agcagatttc agtggtttac gattcaaaaa
    40441 acgtttagct aaatcattta aaataggatc agcagattca gaccattgag taaagtaagt
    40501 gcccaagacc ccatcatcaa gtttgaggta ttcttctaaa gtaaagcttc cgtcaagaaa
    40561 tggcagaagt aattgagaat ggagttcaaa actgctggca tcttcttgat acaactcgtg
    40621 tgcgcgatga agcagatgct ccaaaattac ttccatacca cgggaaacag gatggaagta
    40681 cacttgcaca tacatttgat atcggctaac gatataatct tccactgcat gcatgccact
    40741 catggaaaaa gcgatcccgc cttcataagg acgaatcaca cgtaatattc gggtaagatc
    40801 aaatgttcca tattctgttc cagtgaaata agcatcccga agcaaatagt ccattcgatc
    40861 tgcatcgatc tgactagaaa tcatttggac aacttgtggg ttggggtaag ttttttggat
    40921 cacactagct accttttcag gaaaaccttc ttctactcgg ttaagaatct gatacacttc
    40981 tgtttctgga gaagtgatga tctctacagt gatggcttca tgattcgtat ggaagatata
    41041 ttcaaatgta tgagagtaag gaccatgacc gacatcatgc aacagtgccg cacataaagt
    41101 caccagccgt tcctgatcat cccagcctcc gttgcctatt ttttcttttg aaaaatttcg
    41161 agaaaagata tcgcagattc gtcttgaaat ctcataaacg cctaatgaat gagtaaaacg
    41221 gctatgttct gctccgtgaa aagtgaaaga agaagtgcct aattgtttaa tgcgtcgtag
    41281 acgttggact tctttcgaat tgatcaaatc taagattact tgatgctgga catggatata
    41341 gttatggact ggatcacgaa aaactttttc cataggcagc atttgatatt tataaggggt
    41401 tgtcataatg tagctccttt atgttattac gttgttccat tctctggatt tggttgttcc
    41461 attgctccag ctggatttct ttttttagcc agtcggtaga atcctgtaaa tcacttgcag
    41521 aatacagctt ttcaaaagct tttgcaaatg cttctttgac ttcttttaca aaaaggggtt
    41581 ggtcaagcag ttcttcaagt gttgccattg tagcaggatc cactgcagga tagccatttg
    41641 taccaaagtc ttcttttaaa gcagattgat aaaatcgctt catgacttgc ccacgttttt
    41701 cctgattacc gttgacactt aagtacatca taacagaaat cccatttttg atccgacgct
    41761 gagcgatacc ggcaaatttt ttgccatgga tgcttaaatc ataagttcca ggacaataag
    41821 aatgggtgat ttcgaatgct tgtatcggtg cctttggaaa agcgtattta gtcaatagga
    41881 gcatttgctc gtaagcgtca tctatggtta ttttttttgt gtcactttgg gggaaaatca
    41941 aagaaatatt caaaattcct tcatctgata cgatccctaa tccacctgaa tttctgatca
    42001 ctggagcgta acctgcatct tccagtgttt ggatcccgtc acttagaaaa ggagttcgaa
    42061 catccttcat acctaagatg actgtgcagt catattgcca gaaatgtaaa aagatttgct
    42121 ggtgttcttt agaatattcc gttaatatat ctgttaatgc aaaaggggaa aagtcgtttt
    42181 tttgataaat gtgctgatct agtcgaatcg cagggttcat tcaaccggct cctttgtata
    42241 aattcatatt ttctacaata ttatactcta aaatcaaaga gttgttttgg aaaaaaggtt
    42301 ttctgtaaaa atggacaagt tggttgacaa aacaaaagaa actaggaaca atgtagctat
    42361 gagaagtaaa ggagaatgaa taatggaatt atcacaagga acaccgattt caatcaaatt
    42421 gcgtacaaaa gtaaaacagc aaggggaagt gcaagatttt tattttgatt tgaaagggca
    42481 aatggtgaca attggcgata cgctgtacat acgttataaa gagattcagg aggagacggg
    42541 cgatgagatt cctgtaacga tcaaattagt tccagatggt catgtacagc tgatccgtgc
    42601 aggagaaatg cggatgcgtt tgaaatttgg ctataaagaa cggttagaaa catcctatcg
    42661 aacaccatac ggtatgatcc agattgctac ttttacgaaa gaactgcatg ttagtttgaa
    42721 agaccgtccc acagcaggga aggttcgaat cgattatgat ctgttcatgg gaccggaaaa
    42781 aatcggcgaa tattatttga ccttggattt tacagcgtaa atataaaaga aaagtttgat
    42841 tttttgtgtc tgattttgta tgataaggag tagactgtga aaggacgtgt cctgattgga
    42901 aattagtgta tttgaaggtg caaacaaaag cgaattatca atgatcgaag tagcacacgc
    42961 gatcttagaa caacgtggcg atgtcatgga tttttctgat ttagctaacc agatccaaaa
    43021 ctatcttggg aaatcagaca gtgaaattcg tgattcactc gcacaatttt atactgactt
    43081 gaatattgac ggcagcttta tctcattagg ggataatcgt tggggcttgc gttcatggta
    43141 tgctatcgat tcgatcgatg aagaagtaaa ccatggaatg gacgaagagg atgaagatac
    43201 tccacgtcgc cgtaaacgca agaaagtcaa cgcctttatc aatgatgatg aagacgcgat
    43261 cgattacaat gacgatgacc cagaagatac agatctgaca gaagaagatg acgatgatct
    43321 gtttgatgac gatgatgatg aagatgaaga aatcgctgct tataattctg acttgcaaga
    43381 aatcggagca gatgacccga cagatgatga agaagacttg ccaggaaata tcgaagaaga
    43441 tttaagtatc atagacgaag acgatgatga tgaagattat gaagaggaag aattttctga
    43501 ctctgaaaaa gaataaacat tattttctta cgagaggttg tgacagagac gatcagccta
    43561 tcagaataag gaggtgtgac acgatttttg tcacaccttc ttttttgtat ataaaaatcc
    43621 caagtaaata cataaaaaat aaaaaaattg aaacttttag cgcaccttgt acgtcatatt
    43681 aagtgaggca gaaaggagtg gagaatgatt tttactggat ataagaaaaa acggcagatc
    43741 gaaaaatacc gtcacctgct gaatggactc atcgaaactg attatcaaaa aatgttccgt
    43801 atcacgatga attacgtaca tgataaagaa gaggccttag atgtgatgca agatagtttt
    43861 cataaagccc tgaactattt tgaaaaagaa aaagagatcg agcattttag tgcatggttt
    43921 tatcggatat tgatccgcac ggcattagat agttggcgca gacaaaaaag gacgcctgtg
    43981 gacttatttg aaattcaaga tatcccccaa cagttacctt tagaaacttc cgttgcagag
    44041 ctccatatga ttttagataa aatagacagt ccggaaaaag agattctgat cctctacttt
    44101 tttgaaggat ttcgtttgaa agaaattgcg actatcttgg atatgaatga aaacacagtt
    44161 aaaacgaaaa tgtaccgctc attgaatgct ttacgtcaaa tcctagaatg aaggagagaa
    44221 gagtgccatg tattcccaaa aagaaaattt aaaaaaacta aagaaaactt ataaagaaca
    44281 agcaattccc aaagggctta aagaggagat ccgccagcgt tttattatcg aagagcaatc
    44341 atttataaaa gcaaagcgga gaaagagacg tctccaaggg ttcggagtat ctttggctat
    44401 cgtaatgatt acaacaagta gccttctttt taatagtcaa gttcgaagct ttgcagaaga
    44461 tttacccatc ttaggatcag tcattgaatt gatcttaggc gaacgcttta ctgatcgatc
    44521 tgaaaaaatc gatattcagg taccgaagat caatacacaa aatgagaaag aaaataaaac
    44581 aattcatgga ttaaatcaaa aatactttcg cgaaggacaa gcagattttg aaaaagctga
    44641 aaaagaatat ggaaattttg aaacggatca ttaccaagtt ttgggagatt atcagaaaat
    44701 cgtagacgat aaccggtttc tcgtgattga aaggcagatc acccaaacag cagcagatag
    44761 ccatgttgaa aaaagatacg acacaatcga caagaaaaac agtgtccagc tctcacttcc
    44821 acttttattc aaagatgaca cctatctatc tgttttgaca aaagaagtca aacggcagat
    44881 ggccgaacaa gtcaaggaag atccatctaa gtactattgg actgagcaag atattcaaga
    44941 agggacgata gaaaaaccaa cacttgttac gcctacacga agtttttatt tgaacaaaga
    45001 gcatcaatta gttttgactt tttctcaata cgaaattgct cctggttata tgggtacacc
    45061 agaatttgtc attcctaagt ccgtgacaaa aacgattttg gcatcggaag attatttgga
    45121 tcattagacg agaagtagtt gcgactagca gtttcaattt tcaagtaagt acaagtaaat
    45181 aataagaaga gaaaaagaga aagaagagat atagtgaaaa gaaattattt agttatcagt
    45241 attattgctt tatccgtgct tttgtttttc gaaattactt cggtggcctc cttgatcacc
    45301 gaagtaaaag gacaaacaca ctcttcggag cattcgtcac aatctttcaa acaatcagaa
    45361 caatcaacca ttaaaagcca atcaactgaa acagctaacg tcaaagaagc cattcaagaa
    45421 tcagcggaaa aggtacaaag tcagtcatca caatcaacac aagacagtac aaaaacgagc
    45481 cgagatccgg ctgtcaaaag cgtcgcaatc agctttgatg atggaccagg agcgaccaca
    45541 acaccgcagc ttctgcgaat cttaaaagaa aaaaacgtgc atgccacttt tttcgtctta
    45601 ggagaaaata cggcgcagca tcctgaaatc gtgaagcaga cagctgaagc agggcacgag
    45661 attggcaatc acacatacga tcatcagaat ctggccactc tttctgccca gtcgatgacg
    45721 gaggaagtga cgaaagcaga cactgagatc aaaaaagtca ctggcaaaac ccccactttt
    45781 gttcgtccgc cctatggttc tgtcacgaat gtaggagcaa cgatcatcca gcgaccgatc
    45841 atcgaatggt ctgttgattc agaagattgg aagacaagaa atcctgattt gattttgcag
    45901 aagatccaag caacagtata cgatggggca attattttat tccatgatat ctatcctgaa
    45961 acgattcgtg ccgttcctca agtgatcgat tatctgaaag agcaagggta tcgcatcacg
    46021 acagttgggg atttattagg gcatccaacg gctgtcgaga actattatgg cagaaacgat
    46081 catcggccag ttcagtaagt gagacaagta tctttaataa ataacacatt ttaataagta
    46141 gttgaacaca taactacctt acttgcaaag gcttaacaat atctatacag gtacaacaaa
    46201 aaactgtcca ccaattttct ttggcggaca gttttcactt aaaaataaag aagcaactat
    46261 tcgtaaatta tgctaaaata gcagtagaac gcgtcctgag ggaatcgaac ccccgtctca
    46321 agaaccggaa tcttacgtga tatccactac actaaggacg caagcactgg gataattgta
    46381 actaaacaga taaaaaatta caaggggatt ggacaaaaaa atgaaatttg agcaaaattt
    46441 ttcacaaaaa cagcagcaga cacagaaact agccatgacg caacagttgc aacagtccat
    46501 ccaagtttta caatataaca gtgaggagct gttggcattc gttgagaatc aagcaatgga
    46561 aaatccattg gtggaagttg ttgagccgga atggcagcca gattacataa aagcttcttc
    46621 atcttcttat gaaggagaag agacaaatta cctaaatcag attcctgata caaaagggtc
    46681 tttatttgat tcattgatcg aacaagtcca tttgaattac cgagatactt ttttgaggaa
    46741 aatcgttctt tatcttgtag aatacatcga tttgaacgga tttttgacga ttagtttaga
    46801 agaagccgga aaagagattg gagctacgcc tatccaaatg ctcgatgcgt tgactctgat
    46861 tcagcaatta gaaccagctg gagtaggtgc acgtagtttg caagaatgtc tcatgcttca
    46921 aacagaacga gatgattatg cgccggaatt agcttatatc gtgttggaag aatgttttga
    46981 ggaactggtg gagcgaaaat ggaaagaaat cgcacaacgt ttttctgttg atctacatgc
    47041 tgttcagcag atattcgact atttacaaac gttgagtcca agccctggcc ggattttcga
    47101 ccgtagcagc gaattgtaca ttattcctga tgtccgggta ttagtggacg acgataaaaa
    47161 tgtacaagtt atctcaaacc gaaaaaatca gccaaatata cgctttcaag agagttactt
    47221 caaacaaatg tcacaacaag cagataaaga aactgaaaat tatttaaaag aacggaaaca
    47281 ggaattcgaa tggttgaaga aaacgatcct acaacgtggg gatacaattt tgcgggttgc
    47341 tcaagtcatt gtttcccggc aaaaagcttt ctttatcgac aaggaacgtc caatcaagcc
    47401 tcttacgtta aaggaagttg caactgagat agatgtgcat gaatccacgg tcagtcgtgc
    47461 agtcaacggg aaatatttgg aaacagactt cggtgtattt gaattaaaaa aatttttcac
    47521 tacacgaatt cctacgaata gtacagaaca gacagaagac ctttcagcag atacagcgaa
    47581 gaaaaagctg caggaattag tcgatcaaga agacaaaaac aagccgttgt ctgaccagaa
    47641 attggttgaa ttgctgaaaa aagatgagat agcgatctca agacgaacag tagcaaaata
    47701 tcgtgattta ttaggaatcc cgagttcatc aaaaagaaaa cgttatgata actaatcagt
    47761 caaaagacta gaaaaagtat ttcacttttt ctagtctttt tagttgaaaa acgagagcaa
    47821 aactgttaga cttacagtcg tgaggttgag atttcccttt attgatgtga aattcgaata
    47881 gttaatttct ttttttatcg tatatgggtc gttttaagtc tacattggac gtaaagtgtc
    47941 caactaagga gatcgctatg ctggatgaaa taaaaatgat tgaatctgtt gcaccagata
    48001 ttatcgaagt gttgcaagaa cgctataaaa ttttacgaaa tatctattgg atgcagccga
    48061 ttggacgtcg aagtttatcg gaaagtatgg ggatcacaga acgagtattg cgaacagaaa
    48121 cagatctttt gaaaaatctg aatttgatcg acacctcaaa aagcggtatg acgctgacaa
    48181 ctaagggcga agaagtttat caaagccttg aaaacttcat ggatcagctt ttaggtacgc
    48241 atcaaaccga acaacagttg gcagaatatt ttggtaacca acgctgtatc gttgtttcag
    48301 gaaacagcga agagcagacc aaagtggctg atgcattcgg cgaagcgctg agcgaagccc
    48361 tagaccgact acttccagaa ggcgagaata tcatcgctgt gatgggagga actactatgg
    48421 ctgtggtagc cgaacaattg tctaatctgg aaaataagaa acggcataac ctgtttgtcc
    48481 ctgcaagagg cgggattggt gaagcgataa ctgtacaggc aaattctgtc agtgcaagaa
    48541 tggctgctaa agcaaacggt aaccaccgtg cactttatgt tccggaacaa ttaagtctgg
    48601 caacttacaa ttctttactc aatgagccat ctatccaaga agtcttaaac ttgattagtg
    48661 aagcgaattg tgttattcat agtattgggc gtgcattaca catggcagca cgtcgtaaga
    48721 tgactgaaaa agaattagtc atgctgaaac aagcaaatgc tgttgcggaa tcttttggct
    48781 actttttcaa tgaaaaaggt gaagtcgttt ataaggtccc tagaatcggc atagaattgc
    48841 aagatttgga aaaagtcccg atcatcatgg caatcgcggg cggaaaatca aaagccaaag
    48901 caattcgagc ttatatgaaa aatgcaccaa aacaaacgtg gctcatcact gatgaagctg
    48961 ctgcaaatga gattttaaaa ggggtaaccc tttaaaataa atattttttt gattttcata
    49021 aggaggaaat cttaaatgac agttaaagta ggtattaatg gttttggacg tattggacgt
    49081 ctagcattcc gtcgtatcca agatgtagat ggaatcgaag tagtagcaat caacgacttg
    49141 acagatgcta aaatgttggc acacttgtta aaatacgaca caactcaagg acgtttcaac
    49201 ggaacagttg aagttcatga aggttcattc aacgtaaacg gaaaagaagt taaagtatta
    49261 gctaaccgca acccagaaga attaccatgg ggcgaactag gcgtagatat cgttctagaa
    49321 tgtactggtt tcttcacttc taaatcagca gctgaaaaac atttaacagc tggtgctaaa
    49381 cgcgttgtta tctctgctcc tggcggaaac gatgttccaa caatcgttta taacacaaac
    49441 cacgaaacat taactggtaa agaaacagtt atctcaggtg cttcatgtac tactaactgt
    49501 ttagctccta tggctaaaac attgaacgac aaatttggtg ttgttgaagg attaatgaca
    49561 actatccacg cttacacagg tgaccaaatg actctagacg gacctcatcc taaaggtgac
    49621 ttccgccgtg cacgcgctgc tgctgcaaac atcgttccta actcaacagg tgctgctaaa
    49681 gctatcggtt tagtaattcc tgaattgaac ggtaaattag acggagctgc tcaacgtgtt
    49741 cctgtaccaa caggttcatt aacagaatta gtaacagttc ttgaaaaaga agtaactgtt
    49801 gacgaaatca atgcagcaat gaaagaagct tcaaacgaat cttacggata caacacagac
    49861 gaaatcgttt cttcagatat cgttggtatg acttacggtt cattatttga tgctacacaa
    49921 actaaagtaa tgacagttgg cgacaaacaa ttagttaaaa ctgttgcttg gtacgacaac
    49981 gaaatgtcat acactgcaca attagtacgt actttagaat acttcgctaa cttataagat
    50041 agacaaacgt acaagtgtaa attatagaca tgtacattta aaagcgggga agcaatcgcg
    50101 cttcttccgc tttttttgat aataagttta ttcaggagga aataaaatgg ctaagaaaac
    50161 agtgaaagat atcgatttaa aagacaaaaa agttcttgtc cgtgttgact tcaacgttcc
    50221 tttgaaagac ggcgtgatca ctgacgacac tcgtatcaaa gcagcattgc caacaatcaa
    50281 ctacgttcta gaacaaggcg gaaaagcaat ccttttctct caccttggac gtgtaaaaac
    50341 agaagaagat aaagaaggaa aatctttagc tccagttgca aaacgtctag gcgaattatt
    50401 aggtaaagaa gtaacattcg tacctgaaac tcgcggagaa caattagaag aagcaatccg
    50461 caacatgaat gacggcgacg ttgtcgtatt cgaaaataca cgatttgaag atattgacgg
    50521 taaaaaagaa agcaaaaatg atacagaact aggtaaatac tgggcttcat taggcgatgt
    50581 atttgttaac gatgctttcg gtacagctca ccgtgcacat gcttctaacg taggtatcgc
    50641 ttcaactggt atcccaacag ttgctggatt cttgatggaa aaagaaatca agttcatcgg
    50701 tgaagcagta gaagaaccaa aacgaccaat gattgcaatc cttggtggcg caaaagtttc
    50761 tgataaaatc ggcgtaatcg aaaacttgat tcctaaagca gataaaatct tgatcggtgg
    50821 cggaatgact tacacattct acgaagcaaa aggaatcaag atcggtaact ctctagtaga
    50881 agctgataaa gtggaactag caaaagaatt aatcgaaaaa gcaggcgaca aacttgtctt
    50941 gccaatcgac aatgtctgtg caccagaatt ttcaaatgat gttgaaacac aagtaatcga
    51001 aggagacatt cctgatggtt tgatggcttt agacatcggc ccagcatcag taaaattatt
    51061 tgctgataca ttacaaggtg caaaaacagt tgtttggaac ggacctatgg gtgtatttga
    51121 aatgagtaac tttgctaaag gtacaatcgg tgtttgtgaa gcaatcgcta acttagaagg
    51181 cgcaacaaca atcatcggtg gcggagactc agccgcagca gcagaacaat taggatttgc
    51241 tgacaaattc acacacatct caacaggtgg cggcgcaagc ttggaattgc ttgaaggtaa
    51301 agaattacca ggactagcag caatcaacga taaataaacg accaagtttg ctcaattctg
    51361 acgagtaatc gatcaaaagg tgaagggagt tttcatgact ccctagccat ttttcaataa
    51421 attaaggagt gtgttcctca atgcgtaaac ctattatcgc aggtaactgg aaaatgaaca
    51481 aaactgcatc agaagcaaaa gaatttgctg aagcagtaaa aaacaaaatc ccttcaaatg
    51541 acaaagttga ttcagtcatc ggatctcctg cattattctt acaagaatta atgacatcag
    51601 ctgaaggtac agaactaaaa atctcagcac aaaactgcta ctgggaaaac tcaggtgcgt
    51661 tcactggtga aacttcacca gcagctcttg ctgatctagg tgttcattat gtgatcatcg
    51721 gacattcaga acgtcgtgaa tatttccatg aaacagacga agacatcaat aaaaaagcga
    51781 aagctatctt tgcaaatggt atgacaccaa tcctatgctg tggcgaatct ttagaaacct
    51841 atgaagcagg caaaactgct gaatggatcg aaggacaaat tacagctggt ttagctgatc
    51901 tttcagctga acaagtgtca aacatggtta ttgcttatga accaatctgg gcaatcggta
    51961 ctggtaaatc agctgacgca caaatcgctg acgaaatttg tggagttgta cgtcaaacag
    52021 ttgaaaaact ttatggaaaa gaagtatcag aagcagttcg tatccaatac ggcggttctg
    52081 taaaaccaga aaatattgct gaatacatgg caaaagaaaa tgttgacggt gctttagtcg
    52141 gtggcgcaag cttgcaagca gattcattct tgtcattgtt agacgctgta aaataattgt
    52201 tgaaaatttg cgaaaaatgc aagaaatgtt tgcactaatg agcaaaatta actaaaatcg
    52261 tatatgaggg aaatccctta ggaaaaaaca aactcaaagg agagacaaaa catgtcaatt
    52321 attactgatg tttacgctcg cgagatctta gactcacgtg gtaacccaac aatcgaagta
    52381 gaagtataca cagaaagcgg agcttttggc cgcggaatgg ttccttcagg tgcttcaact
    52441 ggtgaatacg aagcagttga attacgtgac ggagacaaag ctcgttatgg cggtaaaggt
    52501 gtaactaaag ctgtcgacaa cgtaaacaac atcattgctg aagcaatcat tggctacgat
    52561 gttcgtgacc aaatggctat cgacaaagct atgatcgctt tagacggaac tcctaacaaa
    52621 ggtaaattag gtgcgaacgc tattttaggt gtttctatcg ctgttgcacg tgcagctgct
    52681 gactacctag aagtaccttt ataccactac ctaggcggat tcaacacaaa agtattgcca
    52741 acaccaatga tgaacatcat caacggcgga tcacatgctg ataactcaat cgacttccaa
    52801 gaattcatga tcatgcctgt tggcgctcca acattcaaag aagctctacg tatgggtgct
    52861 gaagtattcc acgcattagc ttcaatcttg aaagctcgcg gattagcaac ttctgttggt
    52921 gacgaaggtg gatttgctcc aaaccttggt tcaaacgaag aaggttttga agtaatcatc
    52981 gaagcaatcg aaaaagctgg ctatgtacct ggtaaagacg ttgttcttgc tatggatgct
    53041 gcttcttcag aattctacga caaagaaaaa ggtgtttacg tactagctga ttcaggcgaa
    53101 ggcgaaaaaa caactgacga aatgatcaaa ttctacgaag aattagtttc taaatatcca
    53161 atcatctcaa tcgaagacgg attagacgaa aacgactggg acggattcaa gaaattaact
    53221 gacgtattag gcgacaaagt tcaattagtt ggtgacgact tgttcgtaac aaacactcaa
    53281 aaattatctg aaggtatcga aaaaggaatt gctaactcaa tccttatcaa agtgaaccaa
    53341 atcggtacat taacagaaac tttcgaagct atcgaaatgg ctaaagaagc tggctacaca
    53401 gcagttgtat ctcaccgttc tggtgaaaca gaagattcaa caatctctga tatcgctgtt
    53461 gcaacaaacg ctggccaaat caaaactggt tctctatcac gtactgaccg tattgctaaa
    53521 tacaaccaat tactacgtat cgaagaccaa cttggtgaag ttgcagaata caaaggtttg
    53581 aaatctttct acaacttaaa aaacaaataa ttcttttcta atgaagatac atgatcgact
    53641 attgaaagat agtcgagtat gatcacttag ttaagtaatc gaactctcta cttgctgaaa
    53701 tatcagcagg tagagagttt tttttgcaac tagcttctgt atatgatgtt tacaaagaat
    53761 ggtacaagaa aagatcatcc ttgaaaaagg aaagctaaca ataactagca tcatataaga
    53821 gcttttaagg atagaacagt agaataggaa tctatgagca agtcctctcg tataaataaa
    53881 ataggaggga agtagcagca tatttttcat aaagtggctc tctttcttca tttttacttt
    53941 ggttgctcct atttgaaaat tcgagcacac tcaaatacaa taaaaagaaa tggcaaggga
    54001 gaggattaga tgaaaaaaga agaaaaaatc gcgattttac aagagatcat ccgaattaaa
    54061 tcagtgaatg gaaacgaagg tgaagtagca gcctatttga ataagctttt ggcaaagcat
    54121 gacataacag gagagattgt ttcttacaga gatggtagag ataatttgat tgctcgttac
    54181 caaaagggcc aatcaggaaa agtgttaggc ctatctgggc atatggatgt cgtggcggct
    54241 ggtgatgaat cgtcttggac ctatgcaccc tttgcagcag aaatccatgg gaaccgtttg
    54301 tacggacgtg gtgctacaga tatgaagagt ggcttggcag caatggtgat tgccatgatc
    54361 gaactgaaag agtcaggaaa accatttaac ggaacagtca aattattagc cactgtcgga
    54421 gaagaagtgg gtgaactagg tggggaagag ctcacgaaag caggctatgt agatgactta
    54481 gatgccttga tcatcggaga gccaactaat tacagtttga tgtatacgca catgggatca
    54541 atcaactata cagtcacctc gcacggaaaa gaagcgcata gctctatgcc agaccaaggt
    54601 tataatgcaa tcaatcacct aaatgaattc attacgaaag caaatgctga aatgaatcac
    54661 ctcgctgaaa caatcgagaa tcctgtccta ggaaaaacaa tccataatgt tacattgatc
    54721 agtggcggga atcaagtcaa cagcatccca agccatgcgc agctccaagg gaacattcga
    54781 tcgattccag aatacccaaa tgataaaatc attgcattgc tgcaatcaat cgtcaatgaa
    54841 ttgaatcaag agacagacta tcatttggaa ttgatgatcg attacaacaa aatacctgtc
    54901 aaagctgatc cagattctcc tttgattcat tgcattcagc aacaattcag ccagccatta
    54961 cctttagttg gagcggcagc aacaacggat gcagccgaat ttacgaaagc taaccattcc
    55021 ttcgactttg tcgtctttgg gcctggtgtc gttactttgc ctcatcaagt agatgagtat
    55081 gttgaaatcg ataattattt ggatatgatt gaaaaatatc aaggaattat tttatcctac
    55141 ttagcgtaag atttttttaa taaatgaaaa aacagagcca gattagcatt attttgctaa
    55201 tccagctctg tttattcagc ctatttttct ttttgaatat atcaagcgtt gtggcaacat
    55261 agtgatgact aatgaaacaa cgacgataga caacaaacga tcaaggtagt ctgtacctgc
    55321 ctgtaccaag atcacacttg ttgtctgact taaacctaac ccatgtaata gctgaacgat
    55381 catactggat cctgaggagg tgatcccgcc aaataactga acggtaatga tcgaagaaac
    55441 gatcgttcca ggtaccgaca aaatcaaagc gagtaataac agtttcaagc tgtttttatc
    55501 tttgccgaga tatcttggca aaatcaatcc agcaagcaag cctgtgagaa tttggattgg
    55561 cgaataatac agagaaaaaa tatctgtagt cactccactt aaaataccgc tggacaatcc
    55621 tgtcagcatt ccaggtacag gcccaagcaa tgcaccagca aaaatcgttc cgatagaatc
    55681 gagatagatt ggcaaacgaa gccataaagc aatcgttcct ccaatataat tgatcccaat
    55741 acacaaagcc atcactgtaa gtacgcgagt tgccattttg tttttattca ttttgttagc
    55801 ccatctttaa cctttccaaa tctttcataa tcgtttcttc ttgtgcattt aaaagaacgg
    55861 ttaaaaactt tcggaaaaaa agacgagtat ctacttttgt cataatttta ctgttagcag
    55921 gtttctgcca aaattcataa cgatcaacca atgtttgtcc acgacttatt ccttgagttt
    55981 ccacagcagt gaagcttttg aaaccttcac agatggcccc ctcgataaaa taagcaacag
    56041 ctaacggatc attgatgaca catcctaaaa ttctttcttg tttccagtgg aagtcaaaat
    56101 aaaaacgagt gatcgctttt agatactctc cctcttcagg attcatttga cagcagtatt
    56161 cgaggatagt cggtgtcaag acgatttctc ttgtcacatc taacccaacc atttctatcg
    56221 tttgtttgag attctcgaat acataagaag ctgcttcagg atcacaccag taattgtatt
    56281 cagcaactgg cgaacagtta ccatggcttt tgtaagttcc acccatcgag acaaaacgat
    56341 ccatatgttt cccaatattt gggttgactt tcaaagcaaa agcgatattg gtcaaaggac
    56401 ccaaagcaat gactgatgta tttgtctttt ctttgaaata atcagccaaa aaatcaacgg
    56461 catggagagg ttctgcctgt tttctcaaga tcatcgggaa atttgtttcc cctaaaccat
    56521 ccataccatg agtgtcttgt gcactgatga aaggttgctt caaaggtttt tccattcctt
    56581 gataaacagg aatgtctaaa cgatcacagc gttccaggca tttcagcaca ttctgcgtac
    56641 ctatatctgc agggacattc ccacaaacta cggtaatagc aactacttcg atatcagggg
    56701 atttgacagc taatgataaa gctaaagtgt cgtcgatccc tggatcgcaa tcaataatta
    56761 cttttcgcat tatttccaat ctcctttctt tagagatact tagtttttta ttctgggaag
    56821 tttccgaacc agtccaagac gagcgtcttg attataatgc acgaaattag tatactggct
    56881 tttattgcga atgtaaatgc tctcttacaa gtaaatctat tgaggagaac tttaagtaaa
    56941 tgatacaatg aaaaagaaat ggaatttacg tgtgccgaaa ataaagaatc ctatggatcg
    57001 gtacattgga taatagttag attatagttt ggcagaaagc ggcgggtgta caatggttat
    57061 tttttatttc attcttattc tgataattat agggatcagt taccattttc tttcttattt
    57121 ttatgcaaag aaattagtaa gagcgggagt acagagagga tatgactggt atgaagacac
    57181 tggtcatcgt ttgatggacc ctgctgcagt cacgtcagta gaaaaaaaac gacaaaaatt
    57241 agaagaagag tgtgaaagct tttgggagca gggaacaccc aaaacaatca aaagttatga
    57301 cgggttgaaa ctagctggtc agatgttcat ccatccaaat aagcagaata aatgggtcat
    57361 ttgcgtacat gattaccgaa gcacagggaa aagagacatg tctcatatag gtaaaagata
    57421 tgcagagaaa ggattcaacg ttcttgtacc tgatttacgt gcgcacggag aaagtgaagg
    57481 agaaatcatc ggaatgggat ggctagatcg gctagattta attgcctgga tccaactcat
    57541 cttagatgaa cagccgaatg cgtccattat tttacacggc ggatcaatgg gagcttctac
    57601 gatcatgatg gcaagtggtg aaaaacttcc atcagcggtc aaaggattta ttttagacag
    57661 cggatatgtt tctgtttatg cagaattccg atatatgtta agtaaaatca ctgtctttcc
    57721 taaaaaaatg gtcatgagat atgcgaatca ttatgcccag aaatacgccg gttactcctt
    57781 aaagcaagca tcagctacga gacagcttgg cagcaaccat ctgcctttat tggtcattca
    57841 tggtgaaaga gatcattttg tccctacaga agcagcgtac acgattcaaa atgctacggc
    57901 aggggataaa gcactgctgc ttgttccaga agcagaacac ttggaagcat cgatgaaaga
    57961 tccgaaaacc tattggacag tgattttttc atttattgaa caaagaataa cgatgaatta
    58021 attttctctt cttttttgta agtaattcga ggataaataa ttgtttatag atcaggagac
    58081 tgctaaaata aggtaggaat aaaaaaatat cagttatttg aggagtgcgg catatggaaa
    58141 tcaaagaaga aaaaaatcgt cttgctttat tcaatgatga acaacaagag atcggagaaa
    58201 tgacatggtc agatgcgggc ccagacatca tgatcattga ccatacattt gtagatccag
    58261 catatcgtgg tcaaaagtta gctgaaaagc tagtgtatac tggagtagaa ttagcaagac
    58321 gagacgggaa aaagatcatt cctttatgca cttatgcaaa aaaagagttt gaaaagaaac
    58381 cagaatatca agacgtctta cgtcagtcat aagaataaca gctgaaagtt ttgaactttc
    58441 agctgttatt ccatttatat agagttgtca cggactttta tctatgataa agttaaagag
    58501 acgatatctt ttgggaagga ggctgcgata tgtataattt aattttggga atcgtgatcg
    58561 ttttatcgat tgttatggtc attgcgatta tgatgcagcc aagcaagcaa aatagtgcag
    58621 cgagtgcatt taccggcggt gcggatcaac tattcggcaa acaaaaagct cgcggctttg
    58681 aagctgtcat gcagcgatca actgcagtta tgggagctat ctggatgatc ttattatttg
    58741 tcctagcata tttatcttcg aagtagttga ataagcctct cgcaaaacgg cgggcggctt
    58801 tttttattgc aaagagaacg cttaagaaga agttatgaaa gaaactatgg taaaatgagg
    58861 cgaaagaggt gagacgagtg agacaattac caaaacccct ttatgcaaaa catggaaagc
    58921 gagcggtttt gctgcttcac gcttactcag gaagtccaaa cgatgttcga atgctcgcac
    58981 gatttttgga aaaagcagat tatacagttt atgcgccgct ttttaaagga cacggaacaa
    59041 tggagccata tgatattttg caagaaaaag cagaaagctg gtgggcagac actaaaaaag
    59101 ccatccattt tttacagtct gagcagtttt ctgatattgc cgtattagga ttatcaatgg
    59161 gcggcatctt tgctgtacga gcactagaag aagaatcagt gattggcggc ggttttttct
    59221 gttcgccttt gtctcctgta aaaacaaacg tgcctgaaaa ctttgaaaaa tacgtacgtc
    59281 aagtgctgaa gacagcaggt aaatcagaga aagaaatcaa tgaaaaagca gttgcttacc
    59341 gctcgttagc tgaacaacaa ttaatggata tacaagatca ggcagcaatc gtagaaagta
    59401 ggttgtcaga tatccaacag ccgatatttt tagctcaagc tgggaaagat gaaatgatcg
    59461 accctaatgg cgtatttgaa actgcacgca agttgagccg tcagcgagtg acacttcgat
    59521 ggtatcctga aagtggtcat gtgattacag ttggtacagc aagacgcgaa ttagaaaaag
    59581 acgtcttgga atttttagaa aaattacctt ggaatgagga ataaatatga caaaagaaac
    59641 gataaaagag aaaattctct ttttcatgga aagcagtcgc aaacgcagtt tttctatgga
    59701 agaaatcgct gaaggtttag ggttccaaaa aagcgatgat tttaaattac tagtacaaac
    59761 gattgctcaa atggaaagag aacagagtat agttttcaat aaaaaaggaa aagtgaaact
    59821 tcctttaaaa ccaatcttga tcgaaggaac atttcgagca aatgaacgag gatttggatt
    59881 tgtaacgatt gatcccgaag aagatgacat ctatatccca aaagaagcga ctggttttgc
    59941 aatggatgga gacaccgtag caatcgatat catgaagcga gcaaatacag atatggatcg
    60001 cggagcagag gggaaagtag tcgagattcg agaccgggcg actactcagt tagcagggga
    60061 attcgttgct tattcagaag aagaaataca agaaacggat ctttacggcg ttgttatccc
    60121 gaaagataga aagctcaacc aatttaaagt atatgcagca gcagagggag ttcgtccagt
    60181 tgacggaagt attgtgctga tcgaactgac ccattaccct gaaaaagggt atgcgactag
    60241 tttagaaggg ataatcaagc aagtgattgg tcataagaat gaccctggca tggatatcct
    60301 ctcaattgtc gtggctaatg gtatccctac gaaatttcct gatgatgtat tgtctgaagc
    60361 agatgaagtt ccagatcaaa tcagcgaaaa ggatataact ggacgtagag atttaagaaa
    60421 tcagctcatt gttacgattg atggagaaga tgcaaaagat ctagatgatg cagttacggt
    60481 taaaaaatta gataacggta attattttct tggagtacac attgcagatg tttcttacta
    60541 tgtgacacaa ggcagtcaat tggacagaga agcatatgaa agagggacaa gtgtatattt
    60601 gactgatcga gtgattccga tgatcccgca acgtttatcg aatggtatct gttcactgaa
    60661 tccccatgtt ccaaggctga ccatgagttg cgaaatggag attgatccaa atggacaagt
    60721 agtctctcat gatattttcc caagtgtgat ccaaacaaca gaacggatga cctacacagc
    60781 tgtcaatcaa atcttagaag atcaagacga agaaacgatg caacgctatg ctcagctcgt
    60841 acctatgttc caagaaatgg gtgaattgca tcgaatcctt gaaacgatgc gaatcaaacg
    60901 tggcgcgatt tcttttgagg atcgcgaagc aaaaatcctt gttgatgctg aaggacaccc
    60961 aaatgatatc cagctaagaa gcagaggaat tggtgaacgc ttgatcgaat ctttcatgct
    61021 tgcggccaat gaaacggtag ctgaacatta taataaatta aatctgccgt ttatttaccg
    61081 aatccatgaa cagccaaaag aagaaaaaat gcagcgcttc tttgattttg cttcagcttt
    61141 gggcattctg gtccgaggaa cgaaaggaac gatcacacca aaagatttac agcatgtgat
    61201 cgaaggagta gaagacaaac cagaagctgc agttatcaat actatgttgc taagaagcat
    61261 gcaacaagca cgctattcag aagacaattt tgggcattat ggcttagcgg cagattacta
    61321 cacacacttt acatctccta ttcgacgcta tcccgatcta atcgtccatc gtctgatccg
    61381 ttcttacagt gaagataaaa gtgaaagcaa tcaaagttcg tgggctacgg aacttcctga
    61441 aattgcagat catagttcta agatggaacg tcgtgcagta gaaacagaac gtgaagtaga
    61501 ttccatgaag aaagcagaat tcatgatgga caaagtgggg gaagaattcg atgggatcat
    61561 cagctctgtt gtgaaattcg gcctgtttat cgaactgccg aatacagtag agggattgat
    61621 ccatatcaat gaattgaaac aggactactt ccattttgtt gaaaatcatt tagctcttgt
    61681 aggtgaaaga actggtctga cttttaaaat cggacagaaa gtgcgagtaa aagtagtcaa
    61741 agctgatcca gatgaacgag cagtagattt tgaacttgtt tccgcagaag aggttacagc
    61801 gttggaacgg ccaaaaacac gaaatggaaa atcaggaaaa cgcgaaggaa aacaagcagg
    61861 aaaacgccca gaaagaaatg gcaagaagaa caaacgatat agcgatagta agcaaacgtt
    61921 cggtaagaaa aaagggaaga agccatttta caaaggtgtt aaaaagaaga aaaagaaaaa
    61981 ataaaatgag ttggaggaaa cagctatgcc aaaaggcgag ggaaaattaa ttgcacaaaa
    62041 caagaaagct cgccatgatt attcgatcat cgacacgatg gaagcaggga tggtcttgca
    62101 aggaaccgag atcaagtcga tacgaaacag ccggatcaat ctaaaagatg gatttattcg
    62161 cgtccgcaac ggagaagctt tcttgcataa tgttcatatc agtccttatg aacaaggaaa
    62221 tatttttaat catgatccgt tgcgcacgag aaagttatta ttacacaaaa aacaaatcat
    62281 ccggcttgaa aatgaattga aaaatactgg aatcactgtt gttcctttaa aagtctatat
    62341 tcgtaacggc tatgccaagg tattgattgg tctggcgaaa gggaaaaaat cttatgataa
    62401 acgggaagat ttgaaacgaa aagatatcga tcgacaaatt gatcgaacat taaaaaattt
    62461 ctctagataa tttcattatc ctttttcgca tattcggtgc ttttgctttt acttaatgat
    62521 aaaatttgtt agcatttaat ctgataaaac atgattatca taggtttttt cttagatcag
    62581 gagcaccgtg tataaagaaa aattcgcttc gtttttgact gaaataaaaa agtgtctaat
    62641 tttcatgaaa atgttttgaa ttttctgttt catggtggta tgataccgtg tggttagaaa
    62701 ccgattggaa aaaaccagat acgcacatga attgatttta tgtgaatttc ggggaaaaga
    62761 ggtgaaatgg attggatgaa cgctcgctaa cgttccacat tggaccaatt tggtttgatg
    62821 gtacagtttg tataatggtg ttactgacct gtttgattgt tttcttctta gcttactttt
    62881 ttacaagaaa tcttaagttg aaaccaacag gcaaacaaaa tgcgatagaa tgggtcattg
    62941 attttagtcg aggaatcgta acagataatc tgcctagaaa agaattgagc aatttccatt
    63001 tgttggcatt tacattgttt ttatttgttt ttgttgcaaa caacatcggt ttagtaacaa
    63061 aaatcgtttt acctaacgaa acaacacttt ggaaaagccc gacagcagac ccgtttgtta
    63121 ctttaacttt ggcatttata atgatcttac ttactcactt atttggggtg aaaaagttag
    63181 gcttcaaagg atatttcgta aattcgttct tgaaaccata tagttttatg tttccgatga
    63241 aactgattga agaatttaca aacttattaa cacttgcttt acgtctttat gggaatattt
    63301 atgcggggga agttttgcta accttgattg caaaaatggt aataagctta ggctggttct
    63361 cattgccgtt agcaattcca ttagagatgg tctggatcgc attctccttg tttatcggaa
    63421 gcatccaagc atttgtcttc gttactttat ctatggttta tatgagccat aagattgaag
    63481 tggaagaata atattcgttt taaaaacaaa ctactattta tctaatttag gaggaaaaca
    63541 attatgaatt atatcgcagc agcaatcgca atcatgggag cagcaatcgg agccggttat
    63601 ggtaacggtc aagttatctc taaaacaatc gaatcaatgg ctcgccaacc tgaaatgtct
    63661 ggtcaattcc gtacaacaat gtttatcggg gtagctctag tcgaagctgt tcctatctta
    63721 ggggtagttg tcgccttgat tctagttttt ggtgtttaat tgaatcagta ttaaggcaga
    63781 aacaatttgg tttgttactg cttttttaca tgaaaactag aagtacttca aagaaaggaa
    63841 ggctcgccta tgctgaatca attggcaatt gcagaagttg gcaatgcgat gttaggaaat
    63901 gtcatcgttg tcagcggctc atttttgata ctactggctc tcttgaagca ttttgcttgg
    63961 ggaccaatca gcgatatttt gaaaaaacgt gaagacaaga tcgccaatga tttggattct
    64021 gcagaacaat ctcgcatcaa ctcagcgaaa atggaacaag aacgcgaaca acaattgtta
    64081 gcctctcgtt ctgatgcagc tgatatcatc aaaaatgcga aagaaagtgg agaattaagc
    64141 cgccaaaata ttttgaagga tgctcaagaa gaagcagctc gtctaaaaag taaagcccaa
    64201 gctgatatca ctgtagaacg tgattcagcg ctgaactctg taaaagacga tgttgcagaa
    64261 ctatctcttc aaatcgcgga aaaaatcttg aacaaagaat tatctccaga aatgcacgaa
    64321 tcattaatca atcaatatat cgaaggtcta ggttcttcaa atgaaactag ataaatatac
    64381 agtaggaaga cgttacggga aagctttatt tgaactggcg atcgattcaa ataaagcaga
    64441 agaaatttat caagatctcg taagcttacg tcaaatctat gaacaagtac ctggattagg
    64501 taatatgtta agtgatgttc gtctagaacc tcatgaaaaa cgagaaatca tggacaagct
    64561 tgttagtggc tatgatggaa tcgttaaaaa ctttttagaa gttgtttatg cgtataaccg
    64621 aatggatgat cttttgttca tgatcgaaga atatgaacgt cgatatgatg aacatcaagg
    64681 tttgctgttg ggaagtgtca caacagcagt tccactttca gacgaacaac gaatacaatt
    64741 agaaaagaat gttgcaaaaa caatgaacta tcaatcagtc gaattaaaac aaatcgttga
    64801 ttcttctatt attggtggcg cgattgttga agctaaccat cgagtaatcg acggaagcat
    64861 tcgcacgcaa ttagaaaata tgcgtaatca attaaataga tagtacaaag aggtaggtga
    64921 atcgaatggc catcaaagca gaagaaatta gtgccttgat taaagaacaa attgaaaatt
    64981 atcaaaacgt gctttcagtc gaagaaatcg gtaccgtaac ttatgtaggt gacgggatcg
    65041 ctcgtgcaca tggtttagaa aacgcgatga gtggtgagtt gcttgaattt tcaaatggct
    65101 catacggaat ggcgcaaaac ttagaatcaa atgatgtagg tatcattatc cttggcgatt
    65161 tcgagtccat tcgtgaagga gataaagtaa aacgaacagg aaagatcatg gaagttccag
    65221 ttggagaggc cttgattgga cgggtagtta atccgctagg tcagccaatt gacggactag
    65281 gtgaaatcgt tactgacaaa gctcgtccag tagaagcaat ggcaccaggc gttatgcaac
    65341 gtaaatctgt taacgaacca atgcaaacag gtctaaaagc aatcgacgct cttgtaccaa
    65401 tcggacgcgg ccaacgtgaa ttagttatcg gtgaccgtaa aacagggaaa acttccattg
    65461 cgatcgatac gatcatcaac caaaaaggtc aagatatgat ctgtatctat gtagcaatcg
    65521 gacaaaaaga ttctacagtt cgtacacaag ttgaaacatt gaaaaaatat ggcgcaatgg
    65581 attacacaat cgttgtgaat gccggtgcgt ctcaaccagc gccgttgctt tatatcgcac
    65641 catatgctgg tactgcaatg ggtgaagagt tcatgtacaa cgggaaacat gtcttgatca
    65701 tttttgatga tctttcaaaa caagctgttg cttatcgtga actttccttg ctgcttcgcc
    65761 gtccaccagg tcgtgaagct tatccagggg atgttttcta cttacactca cgcttattgg
    65821 aacgtgcagc aaaattaagt gatgatttag gtggcggttc aatgactgcc ttaccatttg
    65881 ttgaaacaca agctggagat atctctgctt atatcccaac aaacgttatt tcgatcactg
    65941 atggacaaat cttcttagaa agcgacttgt tctacgcagg tactcgtcca gcggttgatg
    66001 ccggactatc tgtatctcgt gtagggggtt ctgcgcaaat caaagcaatg aaaaaagttg
    66061 ctgggacttt acgtctagac ttagcaagtt accgtgaatt agaagcattt acacaattcg
    66121 gttctgactt ggatgcagca acacaagcaa aactaaatcg tggacgccgt acagtagaaa
    66181 tcttgaaaca aaaattgcat gcaccgctag ctgttgaaaa acaagtagtg attttgtatg
    66241 ctttgacaca tggattctta gatagtatcc ctgtagacag tattttagac ttcgaacatg
    66301 aattattcga atatctggat accaatcatt cagatatctt tgaaacaatc cgtacaacaa
    66361 aagatcttcc agaagaagaa gcgttgaaca gtgcgattca ggaatataaa gacatgttct
    66421 tagctacaaa aggaaataat tcctctacag aagataaatt gaaatccatt caaaatgcat
    66481 agtgaggtga gatgaatggg tgcttcatta aatgaaatca agacacgtat cgcttcaacg
    66541 aaaaaaacga gccaaatcac gcgggcaatg cagatggtgt ctgcttctaa gttgacaaaa
    66601 tctgaagctt cttcacagaa gtttcaggtt tatgcaacaa aagtaagaga aatcgttacg
    66661 catttgactg ctactcagtt aaatgatatt gcctcagaaa atcctagagg ggatatcaat
    66721 tacaacagca tgttgctcag ccgtcctgtt aagaaaacgg gttatatcgt catcactgca
    66781 gatggcggtt tagtcggtgg atataacagc tcgatattga aacaaacgat gtctatttta
    66841 gaagaagacc accaatcacc cgatgattac gtgatgatcg caattggcgg aactggtgca
    66901 gacttcttta aagcacgcgg tatcaatcta gcatatgagc tgcgcaatct atctgatcag
    66961 ccaagctttg atgaagtccg caaaatcgta aatatggcaa caactatgta ccaaaacgaa
    67021 gtattcgatg aattatacgt atgttataac catcatatca attcgctaac tagtcagttt
    67081 cgtgtagaaa aaatgctgcc aatctcagac ttagatccag aagaagcaac tactttcgaa
    67141 caagaatata tttttgaacc ttcaaaagaa gaaatcttgt ctcagttatt gcctcagtat
    67201 gctgaaagtt tgatctacgg tgcgatcgtt gacgccaaaa cagcagaaca tgctgctggt
    67261 atgacagcaa tgaaaacagc aactgataat gctgctaata tcattgatga gctgacggtt
    67321 tcttacaacc gtgcaagaca aggggctatt acccaagaaa ttaccgaaat tgttgccgga
    67381 gcttcggcac tagaatagta ttgagattgg aggaaaaaag atgagttcag gcaagattgt
    67441 tcaagtaatc ggtcccgttg tcgacgtgga attttcttta gaccaatcct taccagatat
    67501 caacaacgca ttagttgttt ataaaaatga cgaaaataaa tcaaaagttg ttcttgaagc
    67561 agcgttagaa ttaggggacg gagtgatccg gaccattgcg atggaatcaa cagatggttt
    67621 acaaagagga atggaagtca ttgatacagg caaagcaatc tctgttcctg taggtaaaga
    67681 aacattaggt cgcgtattca acgtactagg agatacgatc gatttagaaa cacctttccc
    67741 agaagatgcg gaaagaagcg aaattcataa aaaagcacca gcctttgacg aattaagtac
    67801 aagtacagaa attttggaaa cagggatcaa agttatcgat ttgcttgccc catatttaaa
    67861 aggtgggaaa gtcggactat tcggtggtgc cggtgttggt aaaaccgtac tgatccaaga
    67921 actgatccat aatatcgccc aagaacatgg tggtatttct gtatttaccg gtgtaggtga
    67981 acgtactcgt gaaggtaatg acttgtatta tgaaatgaaa gattcaggag ttatcgaaaa
    68041 aacagccatg gtgttcggac aaatgaacga accaccaggt gcacgtatgc gtgttgcttt
    68101 gactggtttg acgattgcgg aatatttccg tgatgtagaa ggtcaagatg tactgttgtt
    68161 tatcgacaac attttccgtt tcactcaagc tggatctgaa gtatcagcct tgttaggacg
    68221 gatgccttct gcggttggtt atcaaccaac attggcaaca gaaatgggtc aattgcaaga
    68281 acgtatcaca tctacgaaaa aaggttcgat cacatcgatc caagcaatct atgtgccagc
    68341 cgatgactac actgacccgg cgccggcaac agcattcgcc catttggatg cgacaaccaa
    68401 cttggaacgt aaattaacag aacaagggat ttatccagcg gtagatccat tggcttcttc
    68461 ttcaagtgct ttagcacctg aaatcgtggg agaagaacat tataaagtag caacagaagt
    68521 ccaacacgta ttgcaacgat atcgtgaatt acaagatatc attgctatct taggtatgga
    68581 tgaattatcc gatcaagaaa aagtgttagt ttctcgtgcc cgcagagtcc aattcttctt
    68641 gtcacaaaac tttaacgtgg ctgaacaatt tactggtctt ccaggttcat atgtccctgt
    68701 agaagaaaca gtgaaaggat tccgcgaaat cttggaaggc aaatacgatg atcttcctga
    68761 agaagctttc cgaagtgttg gccgaatcga agacgtcgta gaaaaagcga aaacactagg
    68821 ctactagaaa ggggtgcccc atggatcaat atttaacagt aaatgtggta actcctgccg
    68881 gtttggtcta tgatcaccat gcagctatcg tcgttgcacg tacgacagct ggtgagcttg
    68941 ggattttgcc taaacatgca ccaatcatcg ttccattgac gattgatgaa gttcgtgtaa
    69001 aacgaacaga ttcagatacc cacgtcgatt ggatcgctgt taatggcgga atcatggaag
    69061 ttcgtgataa tgttgtttca atcgttgctg atagtgcgga acgtgatatc gatgtatctc
    69121 gtgcggaacg tgcaaaacaa cgcgcagaac gccaaatcga agaagcaaaa gcaaaacaag
    69181 atacgaacga attgaaacgg gcaactgttg cgttgcaccg agcaatcaat cgtatcaaag
    69241 tatctaagca ctcataaaat gagatgcgga tgtttatccg tgtttaagaa cagtaaaaag
    69301 aggttatgac agaagtggtc agcttcaaga cataagaagg aatttacgaa aattgcttct
    69361 caaatttttg tgaaatctca gcttattttt cgaagaagct acttctggaa caccgtatat
    69421 cggaggttgt gaaatcagcg ttttgtcctg agtattagag aaacaaatgg aaaatagttc
    69481 ctcatatttt gccgtttttt gagctaatac cgagggtcag atgattgaac accgtttatt
    69541 tggaataagg gactgcgaca agacttttgt ctcagtccct ttttttgcat atttttagca
    69601 tttatttcct aaaaaatgta aggggattcc tatattttta agtatacttg tttttccttt
    69661 tatacatttt atggtattat cattttatta gttattataa aacaatcgtt agaaaggacg
    69721 tcattatgca agtatacgga attgatgcgg tgcttcgaat cgtgagtcat ttggcgttta
    69781 tctacttagc cttctggtct ttacgttcgt tacgcattga aacttttttc agaccaatgc
    69841 ataatacgca aattagaatg gcaatcgtac ttttttcaat ttttttaggg tacactgcaa
    69901 gtaatttttt cttagaagtt atcgctttat gccgaaatct tttcgttagc ctgtaaattt
    69961 taaaaaaagg tactatttta aaagcaagga tgttataata gaaaagattt tgagtaagtg
    70021 tatgctcatt ggaatttttt ggagggaaca acatggaaga gatcatcgta agaggtggca
    70081 atcaattaaa tggaacagta agaattgaag gagctaaaaa tgctgttttg cctattttag
    70141 ctgctagttt gttagcagaa gaaggtatta cgacattaga caacgttccg attctttctg
    70201 atgtcttcac tatgaatcaa gtgattcgtc acttaaacgt agatgtagat tttgatgaac
    70261 aaaagaatca agtaacaatc gatgcttcaa gacaattaga aatcgaagcg ccttatgagt
    70321 atgttagtca aatgcgtgct tcaatcgttg tgatgggtcc gctacttgcc agaaatggac
    70381 atgcaaaagt agctatgcct ggtggatgtg cgattggtaa acgaccaatc gatttgcatt
    70441 taaaaggatt tcaagcttta ggagcaaaaa ttattcaaaa aaacggctat atcgaagcaa
    70501 ttgcggatga attgatcggt aacacgattt atttagattt ccctagtgta ggtgctactc
    70561 aaaatatcat gatggctgcc gtaaaagcaa aaggaacaac catcattgaa aacgtagctc
    70621 gcgaaccaga aatcgttgat ttagcaaaca ttttgaataa aatgggcgct caagtctatg
    70681 gagctggtac tgaaacaatg cgtattgaag gcgtggatca tttacatgca gtcaaccact
    70741 caatcgtaca agatcgtatc gaagcaggta cctttatggt tgctgctgca atgacacaag
    70801 gcaatgtgct gattgcagat gctatctctg aacataaccg cccattgatt tctaaattga
    70861 tcgaaatggg tgcagaaatc attgaagaag aaggcggagt ccgtgtcatc ggaccaaaac
    70921 atatcttacc tacagatgta aaaacgatgc ctcatcctgg tttccctaca gatatgcaag
    70981 ctcaaatgac agctatccaa ttagttgcag aaggtacaag tgtagtgact gaaacagtgt
    71041 ttgaaaatcg tttccaacat ttagaagaga tgcgccgtat gaacgctcat gtcaaaatcg
    71101 acggaaatgt agcaatcatg gatggaaacc atgaattgca aggtgcagaa gtatatgcaa
    71161 cagacttgcg ggcagcagca gcattagttt tagctggctt gaaagcaaat ggaatcacac
    71221 gtgtgcgtaa cttgaattat ctagaccgtg gatattataa tttccatatc aaactacagc
    71281 aattaggtgc agacgttgaa cgtgtagaca tggatcaaac ttctgctgaa aaaacagcac
    71341 aaacgattgc ttaaggaagt ggctgaatga gtaaccaacg atatatctta ttaactttga
    71401 taaagatttt agtggtgatc ttattgctta tccttctttt tgtggccgga acgatgatcg
    71461 gatatggtgt catcggtggc ggcaatccat ttaaagtatt ccagccaagt ttgtggattc
    71521 atattcgaga cttttttcat tgataaataa gaatcacagt ggttctagtc gatatgaagt
    71581 gagccatgaa gcagaacatg acaaaagaaa acagaaggga acgttgacgt tagtcgcgat
    71641 cccttttttt agtaaaagaa aagaaggaat ctaattgatg aagaagatat tgatttattt
    71701 agttagagga tatcagcgct ttatttcgcc tgcatttcca cctagctgcc gttattaccc
    71761 aacctgttcc aattatatga taaaagcaat tcaaacccat ggtgcactaa aagggacggt
    71821 aatggggact gcacggatct tacgctgtca tccttttgtc aaaggcggta tcgattatgt
    71881 accattgaca ttccaattaa cgcgaaataa gaaagataaa aaagaaccag attataaaaa
    71941 ggttgtgaaa aatacgagta gcactgagcg ataaagaaac aatttggaaa atagcttctt
    72001 atattttgca aaattgtgag ttattgccga agtgttggat tttgaacacc gtcaaaaaaa
    72061 ggttgtgaaa aatacgggta gcactgagcg ataaagaaaa agaggaaaat tattgtcaaa
    72121 gtaacaactc cgcaactact tatatggggg acaaaaaatg atcgaagaac taaacaagaa
    72181 agatttacca tggtccttgt tattagatgc agatcctgat aaagaaaaag ttcaagtata
    72241 tgtttccaga gggagtggct ggatatggaa agaaaaagaa aaaacaatag gtgtactgat
    72301 ctatgtggca agggaaaaag aatacgaaat cgtcaatgta gcagtagccc caacgtatca
    72361 aggaaagggg attggcggaa aattacttga gacagctttt cagaagcttt cacaactcgc
    72421 ttccagtcaa actcagatca tcatacgaac cggcagtact agcagtgcgg cgttacatct
    72481 ctatcaaaaa atgggctatg tggagattgg tcgagtcaaa gactatttta tccatcatta
    72541 ctctgagccc atttttgaaa atggagatca attaagggat caagtaattt tagcaattca
    72601 cttacctaaa aatagcttta actaataaac tttcaattcg agaaaatgct ttcaaatgct
    72661 aaatagatac attacaatag ctttagcaaa caaaaaggag aatattttta tgggaatatt
    72721 agtttttgat tttggtggat cggctgtcaa atatggttgt tgggatggca aagagattaa
    72781 aggtaaggga caatttgcaa cgcctgaatc ttgggaagaa atgaaagcgc aattatttca
    72841 agtttataaa aaaatactcg tttcttttga agtcgaagga attggaatca gctctcctgg
    72901 agtagtgaat aatgagaaac aggtgattga gggaatcagt gcgattcctt atattcatgg
    72961 atttaatatt ttccgagatt tggaaactct gtttcaactc ccagtaacga ttgaaaatga
    73021 cgccaactgt gccggaatgg ctgaatttta tgaaggtgct gcccgtgatt atcaagatgt
    73081 tgcttttgtt gtggcaggaa ctgggattgg cggggcgttg ttcacgaaag gacaaatcaa
    73141 taaaggggca catctttatg gtggagaatt tgggttgatg ttcttaaatg gcgataagac
    73201 attcagtaaa cttggtacag cggtacagat ggcttggcgt tattgtgaac gcaaaggtct
    73261 cgataaaaat tcttataccg gaaaagatgt ctttgaatta gcagaaactg gagatgcaat
    73321 agcaaaagaa gaagttgaga gtttttatac gtatcttaca aagggattat tcagtattca
    73381 attttcgttt gatccagaag tgatcgtatt aggcggcggt gtttctgcaa aagaagggct
    73441 cattgatgag atcaaaagcc gaatgaagaa gctgacagaa caattcgatc tgcatgattt
    73501 cgaaccgcag attttattat gtgaatacag aaacgatgct aatcttgtag gtgcagcagc
    73561 aaactatctc gctgtacgtc aaggggagaa ttaagagctt tagagaaggt gcagcttgca
    73621 taaaagaagt gaatgaaatg aataggagat ttttacctaa ggaatgtttt atttgctgca
    73681 gtatgataaa atatctgaga ggtgaaaatg atgtcaaaaa aaaaataaag atgttgaagt
    73741 gcgtatagaa gaagtgaaaa aagatatcaa aggtaagaat tatgaagtca ctcagttatt
    73801 tatagggaaa aaaatgattg gtgagatctt agcctatggg ccaaaagaat atgaaatttt
    73861 ctttggtgaa gaagattttg gaaaagaaaa aagtcttgaa aatgcgatag aaacagtgat
    73921 tcgacactgg aatctgcacg aataaaaaaa tatcaaaaaa actttcaata aagggttgca
    73981 aaaatctgga aaattaggta ttataactaa gtcaggttga tacaaacctt tcggaggagt
    74041 agcgaagtgg ctaaacgcga cggactgtaa atccgttcct tagggttcag tggttcgaat
    74101 ccactctcct ccatttcatt ggggtatagc caagcggtaa ggcaagggac tttgactccc
    74161 tcatgcgttg gttcgaatcc agctacccca gtataccaca atcgtaagct ctcgttagtt
    74221 cgagagctct tttttttgct ttgctacctt gttccttgaa gattctgctg atttaagata
    74281 gagttaagtt aactacaaga attggaggga tccacatgaa atggaaagaa aaagtagacg
    74341 aagcagctga aaagttatat gatttggtca aatcagaaaa atacaatatt gaagtgaata
    74401 ttccgaaaaa aggcggaaaa gcggtccagg taaaatcaaa acgcccaacg aatcatacaa
    74461 aaaaatggat ggctaaaaac cgctaataat aacaaaagat ccataagtcg gcattttcat
    74521 cgattttagg gtcttttgtt attttctagg ttgaacatat ctaaaaaatt gtttctgaca
    74581 ataggagtag gaagaacgat gtgtgtctaa atcctctgtt gatggataga aacctttatc
    74641 tttttcagtt gtgaatcagg tatataccag ttataataga agaaacgaaa ggacgggtgt
    74701 acgttatgtc gaatcaatta cctgtatata tacagattca tgaccaatta aaaaatgaaa
    74761 tcgaacaagg tatatggaaa gtcggagacc gtcttccttc agaaagagaa ttagcagtga
    74821 aattcaatgt cagccgtatg acgttgagac aagcaattca aaacttggca gatgaaggaa
    74881 tcttagaaag aaagatcggt tcgggtacat atgttgcgcg agaaaaagta caagaaaaaa
    74941 tgtctggtgc aacaagtttt acagaaatta tggaatcgca aaatcgagta ccgtctagtc
    75001 ggacaatctc ttatttcttg acttcgccaa gctcaagtga aatggaaaaa ctaaacttga
    75061 gtaaagatga cacgatcttg cgaatggaac gtattcgttt cgcagatgat gtccctattt
    75121 gttttgaagt tgccagtgtg ccacaaaagc tgatcgatgg ctacagcaaa gcagagatta
    75181 ctgaatcgtt gtatcgcacg ctagaagaaa aaggcgggaa aaagatcggt ccagcgaatc
    75241 aaacgattac tgctgtgttg gcttctgaga aaattgctga ctatttagag gtaaaacgag
    75301 gagatgcagt tcttcgtttg cgccagattt cttattttga agacggtacg ccgtttgagt
    75361 atgtgcgtac gcaatacgta ggaaatcgtt ttgagtttta tttggaaaaa taaaaaacag
    75421 gtgatccctg attccttact tagaaataaa agagaatcag aatcacctgt ttttatatat
    75481 gatcagttac cagttgctcg tgataagaaa caggaccctc ttcaaattgg acttgtccgt
    75541 tcagcagctc aatgatggat tctttaaagt ggtcagcact tttttcatct acagcacaga
    75601 tgacgctaac ttgatcagta tattggattt cttttataaa aaaatgttgg agttcaagat
    75661 aattctgtaa ttttcctagt tgcggatacg tgatcatcac agagatctcc tgttgcaagg
    75721 taccttctac gattccaatg tcagacaatg cttgagagac agcatgagtg tatgctcgta
    75781 ttaatccgcc tgtgccgagt ttcgttcctc caaaatatcg agtgactact gcagctacat
    75841 tgatcagttg attttttttg aggacttcca acatcggtac gccagccgtt ccacttggtt
    75901 ctccatcatc gctgcttcgc tggatttcat ttttttctcc aatgatgaag gcgctgcagt
    75961 tatggttcgc tttccaatgt tcttttttta tttcttgaat gaaacttttt gcttggtcct
    76021 ctgattctat tcgtctaaga gaacagataa agcgtgattt tttgatctca atctcacttt
    76081 gtccattttc ttttattgta cgatagcgct cgatcatctt gtcacctctt tttttcattt
    76141 tctttattca gtataaataa agttctaggt tgtttcaaca ctgtaacggt tgcgcaatcg
    76201 ctgtagtatg cgataataaa gacaaaggta ggtggatctg gggatgcagg aacgaatgaa
    76261 aaagtacgat gcaattacgc attatttgaa gaacaatggc ggctctcaag taacgttgac
    76321 ctttacccaa tttgacgaac tgctgtttcc cagtaacgga cttcccaaaa cagcaaggga
    76381 aagtacagat tggtgggcaa atgattataa acaccctgaa aaaggtgcct atggctggat
    76441 aaatgcaggc tatgaagtag ttgtcatcaa tttggacaaa gaatatgtcg tgttcaataa
    76501 attagtgaaa tctagttggc tatttgatta aataaaccgc gtttagaaaa cgttgacaaa
    76561 tttctttctt tttatataat agagtaacag acgcgaagaa aacgtttgcg tagtttgttt
    76621 taaaaaggag agaaaagtat ggctaaaaaa acaatcatgc ttgtatgttc ggctggtatg
    76681 agcacaagtt tactagtaac aaaaatgcaa aaagctgctg aagcaaaagg aatcgactcg
    76741 gatattttcg ctgtgtccgc ttctgacgca gataacaatc ttgcaaataa agatgtagat
    76801 gtactgttat taggacctca agttcgcttt atgaaagctg attttgaaaa acgtctggaa
    76861 ccaaaaggca ttcctttaga cgtcatcaat atggctgatt atggtatgat gaatggcgaa
    76921 aaagtgttag atcaagcaat cacgctcata ggtgaataaa tagaacagta taactatcga
    76981 acaagcgatc caaaaaatag atcgcttgtt ttttcgtata tttttttagg gcgaataata
    77041 tgaatcattt ttggggtaga aggcttctga cgagagatat taaagcaata gactgtgagg
    77101 aggtaaattt accaaattat gagaagatcg cagcagtgga acgtttgggc aactggattc
    77161 tttgtcatcg ctgtggaatg aggacgtccg tcattgatgg acagcttgca gattacggtt
    77221 atttttgtac acattgtcta tatttagggc gatgcgacac tcaacaagat ttatatttgt
    77281 ttgatcagcc tgaagcaaaa cctcgagaag tcgtcttttt ttggacagga cagcttactg
    77341 aaaaacaaac ggaaatcgca aagagaatac tttgccattc agaaaaaaga catcatctta
    77401 tttgggctgt aacaggtgca gggaaaacag agatgcttta tcctatttta gtggaaactt
    77461 taaaagctgg aggaagagta gcgatttgta caccgcgagt cgatgtttgt aatgaattgt
    77521 ttttacgcta tcgttatgtt tttccagaag aaaaaatcac gttgctccat gggaatacag
    77581 caacggctta tacctttact tcttttgtta tctgtacgat tcatcagcta taccgttttt
    77641 atcggtcctt tgatttaata gtgatcgatg agatcgatgc gtttccttat aacggggatg
    77701 caggattagc tcatgctgtg caaacagcga ttaaaccaga cggacgattc atctacttaa
    77761 gtgccacacc tgataaaaag ttactaaagg aaatcaagca gacgtttgac atccacaaac
    77821 ttgctttgcg ctttcatcgg cgtttacttc ctgaacccgt cttggttttc atgaataatt
    77881 ggcgacaaaa atgcaagaac aagaagaaga taaaatcttt ggtgcggatc attcatcaat
    77941 taattaaaga aaaccatatc cttttatttt gtccaagtat cacgatgatg catcgtttga
    78001 aagccacact tcaagaggct cttccgagat atgagatcac agatgtttct tcaagagaca
    78061 atcagcgggc ggaaaaagtc cagaaaatgc gagaagcaaa atatgacata ctgttaacca
    78121 cgatgatcct tgaacgtgga gtcacatttg aaagaatatc cgttatcgtt ttaggagcgg
    78181 atcatccggt ttttactaaa tcttctttgg tacaaatcgc aggaagagcc gatcgtaaag
    78241 ggccttatac gaacagtcaa gtatactttt tctatgaaga aaaaactgca gctattcgaa
    78301 aagcttgtca ggaaatcaaa gaaatgaatg aagaagggaa gaagatgctg tgaggtgcag
    78361 ttgctgcagt aaagatatcg tccaaaattt aacattcagc gagattattg gtgtttcgaa
    78421 atacagatta cctcagactt gttcagtttg tcgcgcacaa tttcatttat tggaaacaag
    78481 aggctgttct ggctgtcaaa aaccaatcca tttcaatcta gagagtacga aaaagaaagt
    78541 aaagtctatt cccgtttact gcagtgactg tcaagaatgg aagaagaaat atccgaccta
    78601 tgattttcag caccgtgcct tttttgtgta cgatgaaaat atgaaggcct ggctcaatca
    78661 atacaagttt atgggagata tccgactagc aggcaccttt gcatataaat ggacgcagat
    78721 aaaaaaagaa tacccgacat acatctattg tccaatacct ttatctgaaa aaaggcgaac
    78781 agaaagagga ttcaatcaag taagcgagat gttgaaagca gcaagaattc cttttcagat
    78841 gctactcaaa agagagaagc atctgctggc tcaagcaaaa aacacgcgaa aggaaagatt
    78901 ggagatgcct caaccttttg aacttgcagt caccttttct aagatacaaa ataaaaaaat
    78961 attgattatt gatgatgtct atacaacagg acagactctt ttccacgcag cagattgttt
    79021 gcaggtcgca cagccgaaaa tcatccgaac catttcttta gcaagatgaa ttagatgtgg
    79081 aaatttgata gcgatttaat ttgcaaataa cctagagttc gatataatag aattaagaag
    79141 agggaaagag tggtccttct ccttcttgat tgtggtagac gaaaggggta atttttatgt
    79201 ttagatataa tgtacgcggc gaaaatatcg aagtaactga agcgattcgc gactacgttg
    79261 agaaaaaagt aggcaaacta gaacgctatt tcagtgacgc acccgatgca acggcgcatg
    79321 tgaacttaaa agtttataca gaaaaaacag caaaagtgga agtaacgatc ccattacctt
    79381 atcttgtatt acgtgccgaa gaaacttctc cagatttata cgcaagtatc gatttggttg
    79441 ttgacaaatt ggaacgccaa atccgcaaat tcaaaacaaa aattaatcgt aaatcccgtg
    79501 aaacgggcat gaatactgca gatgctactg ctttattcaa tgaagaggac gaaaacgaaa
    79561 atgattctga attagatatc gtacgtacga aacgcctttc attgaaacca atggatagtg
    79621 aagaagcagt cttacaaatg aacatgctgg gacataactt ctttattttt gaagatgcag
    79681 aaacaaatgg tacaagtatc gtttaccgcc gtaaagatgg aaaatacgga ctgattgaaa
    79741 cagactgatc agccttactt aaataacaac gaaaacttgc agcagctact gacagctgcc
    79801 gcaagttttt ttgagtttcc tcctctttta gatgaaatat gaataaatta tgaagttctt
    79861 actttaatac gcttaagata tttcaattat ctataactaa tgataaaatg aaaagtaaag
    79921 tctacatagt ctggataata cacagaagga aaaggagatt acacaacgca tggccaattt
    79981 gattcgttca cttatcgaaa acgataagaa agaactaaaa cgtttagata gcattgcaaa
    80041 aaaagttgaa gcatacgctg accaaatggc agcattaact gatgaacaat tacaggcaaa
    80101 aactgctgaa ttcaagggca gataccaaaa aggagaaaca ttagatcaat tacttccgga
    80161 agcttttgca gtcgtccgag aagcagcaaa acgtgtattg gggttatatc cacaccacgt
    80221 acaattgatg ggtggaatcg tgcttcacga tggaaatatc cctgaaatgc gtactggtga
    80281 aggtaaaacc ttgaccgcaa cgatgccagt atatctaaat gctctgactg gcgaaggggt
    80341 acacgtagtt accgtcaatg aatatttagc aacccgtgac tcgacggaaa tgggcgaact
    80401 ttataatttc ttaggattaa cagtggggtt gaatatcaac tcaaaatctt cggatgaaaa
    80461 acgcgaggct tataattgtg atatcactta tagtacgaat aatgaattag gatttgacta
    80521 cttgagagac aatatggtcg tttaccgcca tcaaatggta caacgtccgc tgaattatgc
    80581 cattgtcgat gaagttgact caatcttgat tgatgaagcg agaacgccgc tgatcatttc
    80641 agggcaagca gaaaaatcca cagctttata tacaagagca gacaacttcg tgaaacgttt
    80701 gaaagaagaa gaagattata aaatcgatgt acaatcaaaa acaatcagtt taaccgaagc
    80761 agggatcgaa aaagcagaag aaacattcgg tttggataac ttatacgaca ttgaaaatac
    80821 tgcattgacc catcacttgg atcaagcatt gagagccaac tatatcatgc ttttagatat
    80881 cgattatgtt gtccaagacg gaaaagtact gattgttgac cagttcactg gtcggatcat
    80941 ggatggacgc cgttattcag atggacttca ccaagcaatc gaagctaaag aaggcgtaga
    81001 gatcgaagac gaaacgaaaa ccatggcgac gatcactttc cagaactatt tccgtatgta
    81061 caagaaactt gcgggaatga ctggtacagc taaaacagaa gaagaagaat tcagagaaat
    81121 ttataacatc caagtatacc agattccgac aaatcgtccg gttatccgtg atgatcgtcc
    81181 agatttgctt tacccaacat tacaaagcaa gttccacgct gtcgtgcaag atatcaaaga
    81241 tcgctaccgc aaaggtcaac ctgtgttagt cggaactgtt gcggttgaaa catctgagtt
    81301 attatcagat atgctgaatc aagaacgtat tcctcatgaa gtgctgaatg cgaaaaatca
    81361 ctttaaagaa gcagagatca tcatgaatgc tggtcaaaaa ggtgctgtga cgattgctac
    81421 caacatggct ggtcggggaa cagatatcaa gttaggactt ggggtaagag aacttggtgg
    81481 tctagcagtt atcgggacag aacgacatga atctcgtcgt atcgataacc agcttcgagg
    81541 acgtgcagga cgtcaaggag accctggtat gtcacagttc tacttgtcat tggaagatga
    81601 tttgatgaaa cgattcggtt cagaacgaat caaatctttc ttagatcgga tgaagatcgg
    81661 tgaggaagat gctgttatcc aaagcaagat gctgacgaaa caagtagaat ctgcacaaaa
    81721 acgtgtagaa ggaaataact atgacacacg taaaaacgtc ttacaatacg atgatgtgat
    81781 gcgtgaacaa cgtgaagtaa tctataaaca gcgacaagaa gtaatcatgg aagaaaaaag
    81841 cttatccaag ccactaatga acatggtcaa acggacaatc agtcgtgtag tagatgcgca
    81901 tacacaatta gagaaagaaa attggaattt ggaaagtatc atcgattttg ccggcagtac
    81961 tcttgtccat gaagatagta ttactcttgc agatatcgaa ggaaaaacag ctcaggagat
    82021 caaagagtat ctaaacgaac gagcacaaga agtatttaat accaaagcgg ctcaactaaa
    82081 tggtccagag caactgttgg aatttgaaaa agtcgtgatc ttgcgtgttg tggactctaa
    82141 atggacagac catattgatg caatggatca attacgtcag tcaatcggct tacgtgctta
    82201 tggacagaac aatccattag tcgaatatca aacagaaggc tataagatgt ttgaagatat
    82261 ggtaggcgcc atcgagtatg aagtcactcg aatctttatg aaagctgaga tccgtcaaaa
    82321 cgtacagcgt gaacaggttt ctcaaggaac agcttctcac tcagaagacg gagatgccaa
    82381 agaaaatagt aatactagcg cgaaaaaaca gccaatccat gtgaacaagg tcggaagaaa
    82441 tgatccttgt ccttgtggaa gcgggaaaaa atataagaac tgtcatggaa aaaatgcaaa
    82501 ctaatttctg acagcaggat tggtgagaca aattcttttt ttaagaattc gtctcacctt
    82561 ctttctgtta tttgctgatt ctgttagact agatataatg aaaaaagtat agtgaaagaa
    82621 ggaaaaaaca tggaaatatc cgaaattcga aatctcctga cagaaatgca ggaaaaggtc
    82681 acaagcttca ggaggtctct ttgacttaga ccagctggaa gaagacattg cggaagcaga
    82741 agcaaaaatg gctgaacctg gtttttggga tgatagcgaa gctgctcaaa aagtgatcag
    82801 tgaaaacaat gcaaataaag aaacttatga tcaattccat tctcttgctg aagaactgga
    82861 agaaatcgaa ttgatggtgg agatgttgca ggaagaacct gatgcagcca tgcaagaaga
    82921 agcagaaaca cgtatcaatg cgctagatga aaaaatgaag acatatgaat tatctatgtt
    82981 gttagatggt ccttatgatc gaaacaacgc catcgttgaa ttgcatccag gggctggcgg
    83041 aacggagtcc caagattggg gcagtatgct tttacgtatg tatacacgct gggcagagca
    83101 gcatgggtat caagtagaga cactagatta tcaagcaggt gatgaagcag gaatcaagag
    83161 tgtgacccta ctgatcaaag gacacaacgc atttggttat ctgaaatcag aaaaaggtgt
    83221 tcatcgcctt gtacgtatct cgccttttga ctcagcgaaa cgacgtcata cttctttttg
    83281 ttcggtagat gtgatgccag agcttgatga aacgatcgat atagaaatca atccagatga
    83341 tttgaaaatc gatacgtacc gagcaagtgg tgcaggtggt cagcacatca ataaaacaga
    83401 atctgctgta cggatcactc acttgcctac aggaacagta gtagctagtc aggcacaacg
    83461 ttcacaattg aaaaaccgtg agcaagcgat ggggatgtta aaagctaagc tttatcaatt
    83521 agaagtggaa aagaaagaac aagaagcagc tgcacttcga ggagaacaaa aagaaatcgg
    83581 ctggggttca cagatccgtt cttatgtttt ccatccatat tctatggtaa aagaccatcg
    83641 gacaaacgta gagacaggaa atgtccaagc agttatggac ggtgaccttg atttatttat
    83701 tgacggctat ttaaaaatgc atctacaata agaacccatt gttaaattga aataaaattg
    83761 taagattttg caagcatgtt gaaacattgc catgctataa tagctaaaga ctgaaaaaga
    83821 tatggagaga acgccatgat tgaaatgaag gatgtaatga agaagtactc aaatggtacg
    83881 accgctatcc gcaatctctc aattgtcatc gaacaaggag agtttgtcta tgtggttggt
    83941 ccgtctggag ccggtaaatc tacattcatc aaattgatgt atcgagaaga aaaagcttca
    84001 aaaggaactt tgaacgtcgc aggtcatgat ctaatagcaa tcaaaaaccg cgaagttcct
    84061 tatctacgca gagaaatcgg cgtggtgttc caagactata aattattacc aagaaaaaca
    84121 gtatatgaaa atgttgcgta cgctatgcaa gttatcggca gaaaacctcg ggatatcaaa
    84181 aaacgagtaa tggaagtgct ggaccttgtt ggtttgaaac acaaagttcg agtctttcct
    84241 agcgaattat ccggtgggga acagcaacgt gtctctattg ctcgggcaat agtgaatacg
    84301 cctaaagtac ttatcgcgga tgaaccgaca ggaaatctgg atccagaaaa ctcatgggaa
    84361 atcatgaaat tattagatcg gatcaatgcc caaggaacta ctgtcgtgat ggctacacat
    84421 aacagtacga tcgtaaatac gattcggcac cgagtaattg ctatcgaaaa cggccgcatt
    84481 attcgagatc aagcggaagg ggaatatgga tacgatgatt agaacctttt tccgccattt
    84541 attagaaagt atcaagagtt tgaaacgtaa cggctggatg acagttgctt cagtcagtgc
    84601 ggtaacgatc acattgactt tagtcggtgt attcatggca gttatcatga acgcaactaa
    84661 attagctcaa gatatagaag gaaacgttga tgtctctgta ttcgtagata tcggtacaaa
    84721 agacgaagag atgaaaaagc tagaaaaaga attgaatgac ttagatcatg ttaaaaaagt
    84781 tgaattttca agcaaagaac aggaattgaa aaaaatccaa aacgaaatgg gtgattcctg
    84841 gaaattgttt gaaggtgaca ataacccgtt atatgatgtg tatgtagtaa gtgctactga
    84901 tccttcttat acaaaacaag tagcagaaga agcagcagat ttgaagaatg tttctcgttc
    84961 tgattacggt ggagcttcct cagatcggat tttccaaatc gctggtgcag ttcggacatg
    85021 gggattagtt gcagcggcat tgctgctgtt cgtagcgatg tttctcattt cgaacacgat
    85081 tcggatcacg attttgtcta gacagcgtga aattcaaatc atgcgtttag tcggtgcaaa
    85141 aaatggatat attcgctggc cgtttttcct tgaaggtggc tggatcggtc tgatcggtgc
    85201 gattcttccg attttgatca tcacatttgg ttatgcatgg tttttccaat tgatcaatcc
    85261 atcattatta cgttcccatt attcattgat tcatccacaa aatatcgttt ggaaaatcaa
    85321 tcttttaatg gtcggcattg gtgtgatcat tggttcactt ggctcgatca tttctatgag
    85381 acgtttcttg aaattctaat accacaatat aaataaaatg agaaagttgg acagtttcgt
    85441 tccagctttc tttttttatt ggctaggaga atagaaggaa gcggcttttt tccctttact
    85501 ttaaaaaaca acttgggtat tcttaagttg gtagaagaag aggtgaagcc gtgaaaatta
    85561 tcgttgacaa tttttttacg atccttttga ttatgaatat tttgctttcc tttatcatcg
    85621 tttttcgaga aagaaaggaa actgctcaaa catgggcatg ggtgctcgtt ttgatgttca
    85681 ttccagttgt aggatttatt ttatatattt ttttaggaag agggatttcc aaagagaaaa
    85741 tctttgattt gaagatccaa gggaaaatcg gtaagaacct ggaaattgaa gaagacaaac
    85801 aggcattgat gcgagggctt tatcctcatc cgcctactgg acaagtagat gtgaaacaat
    85861 tgatctatat gctcacggta tttgaaagta cgctctatac gactaaaaat gaaatcactt
    85921 tatttacaga tggtcgtgaa aaattcgatg cgctgattga agatatcaag caggcagaag
    85981 accatatcca cttccagtat tatatttatc gcagtgatgc attaggtgaa gaagttcgag
    86041 atgcattgac agaggcggca agacgagggg taaaagtcag agtccttctt gatgcatggg
    86101 gttccacaca agtttcttcc agcttttttc agaatttgaa aaaagcaggc ggagaagtcg
    86161 catttttctt tcctctattt gttccgtata ttaatcctcg gatcaattat cgcaatcatc
    86221 gtaaaatcgt agttattgat gggaaaatcg gatatacagg tgggttcaat gttggaaatg
    86281 aatatctagg attggtaaag aaatttggtt attggcgaga caatcatttg cgtatttacg
    86341 gagaagcggt gtataccttg caaaaccgtt ttttgatgga ctggaattct cagcatacaa
    86401 aagaagtagg ctattctccc aaattttttc cgtccattca ctctactggt gatatagctg
    86461 ttcaaatcgt gacaagcgga ccagataccg aacatgaaca aatcaaaatg acttatttga
    86521 aaatgatttc gttagccaaa cgggagatat tgatccaaac gccttattac ataccggatg
    86581 gatcgattca tgaagcactg aagttggctc ttctttcagg cgtaaaagtt catatccaaa
    86641 tacctaacaa accagatcat cttctcgttt actgggcaac gtattctttc gctgcagagc
    86701 taatcgagta tggagcaagg atcgaaacat atgagaatgg atttattcat gcaaagacga
    86761 tgattatcga tggtggcatt gtttcagttg gctcagcaaa tatcgatgtt cgttcatttc
    86821 gcttggactt tgaggtcaat acactggttt atgatgaacg aatggcttca cgtgtaagaa
    86881 aagctttttt tgaagactcg aaaatttcta cgcatctcac aaaagaaatg tatgaaaacc
    86941 gaggaataat catcaagatg aaagaaggat tagcaagatt gatttcgccg ctcctttaaa
    87001 acataaaaag gacatgagaa ccatcaacag atggagatct cgtgtccttt tctaacgggg
    87061 ccatttctga gagtacgtca gaaatggaga aagcgtctcg catgagcgaa attaactttg
    87121 gggtatctct tgttttttgg ggaaagcaag aaataagcta atatatgcta ttaaaagaga
    87181 cacatttcaa agccagtata gcatagaaac ttttaatgag atagtttttt ttacaattat
    87241 gagaatttat tttgttattt aatgattttg attcgttttc atcaagtaat gaaaatgata
    87301 aaataaacat actttaggat taaaggagat ttctaatgaa aaaacttaaa ttattcttac
    87361 caataatagg attagctatt cttttatcag gctgttcaaa gtggattgat cgaggagaat
    87421 ccattacggc tgttggctct tctgctttac agcctttagt agaaacagtt gctgaaactt
    87481 accagacaga aaatcctgga aaattcatca atgtacaagg gggcggaagc ggaactggtt
    87541 tgtctcaagt acagtcagga gcagtcgaca tcggaaactc tgatttgttt gcggaagaaa
    87601 aatcggggat cgatgcctca gcattagtcg atcatcgagt agcagttgtt gggatcactc
    87661 ctattgtgaa caaggatatt ggcgtaaaag acatcagtat ggaaaacctg aaaaaactat
    87721 ttacaggcga gatcaaaaac tggagtgaag tcggtggaaa agatcagcca gtagttatat
    87781 tgaaccgcgc ttcaggaagc ggaacccgta gcacgtttga aaaatgggta ttggacggag
    87841 aaacagctgt tcaggcacaa gaacaagatt caagcggaat ggtgcgccag atcgttgccg
    87901 acacacccgg tgcaatcagc tatgttgctt tttcttatgt ttccgatgat gtagatacgt
    87961 tgaatgttaa cggtgtagaa ccgacagatg aaaatgtgac aacgaacaaa tggacgattt
    88021 gggcatatga gcatatgtac acaagagatc atccaaaaaa actgacaaaa gaatttttag
    88081 actatatctt atctgatgaa gtacaagata acatcgttgg agaactaggt tatatccctg
    88141 tttctcatat gaaagtcgaa cgtgattggc aaggaaatat cgttaaagag taatctttac
    88201 acaaaattta caaacttagc taaactttta catgttatta aaaccaaatt gatgttcaat
    88261 ttgtacactg agtgagtatg ttaaacctga ttaaggggga gtcatctgtg gaagatgtga
    88321 aaaaagtatt attaacaaaa tcaaaacgtt caaaaatgga acagcgtgga aaattcatca
    88381 gttttttatg tatcgcgctg atcgtgttgg ttgtattatc tattttttat tttgtagcta
    88441 gtaaaggatt ggccaccttt tttactgata agatcaatgt atttgatttt ctgtttggta
    88501 cagattggaa tccaagccaa atcggagcaa atgggaaacc aatggtaggg gcattgccta
    88561 tgatcacagg ttcgtttatt gtgacctttt tatctgctat cgttgccacg ccatttgcca
    88621 ttggtgcagc agtattcatg acggagatct ctcctaaaaa cggaacgaag atcttacagc
    88681 cggtcatcga gctgcttgta ggtattcctt ctgtcgttta tggttttatc ggtttatcag
    88741 ttatcgtgcc ttttgtcaga agtatttttg gcgggactgg gtttggtatc ttagctggta
    88801 catttgtgtt gttcgttatg atcttgccga ctgtcacaac gatgactgtt gatgcattaa
    88861 aggcagtgcc acggcactat cgggaagctt cattagcgct tggtgccaca agatggcaaa
    88921 caatctacaa agtcgtgcta cgagcagccg tgcccgggat tttaacagca gtcgtttttg
    88981 gtatggctcg tgcgtttggt gaagccttag ctatccaaat ggtcatcggt aatgcagcat
    89041 tgatgccaac aggtcttact actcctgctt ctacattgac gtccatctta acaatgggaa
    89101 tcggaaatac aatcatggga acagttgaaa ataatgtgtt atggtcatta gcgctgatct
    89161 tgctattgat gtcattattc tttaatatcg tgatccgtat gattgggaag aaaggagagc
    89221 tgaaataatg aacgcaaaac gctcagataa aattgctaca ggtgttttat atatagtttc
    89281 ggggatcatc gtattgattt tagcagcttt actactttat atcttagttc ggggaatacc
    89341 gcacatttca tggtccttct tgaccgaacc ttccaaagcc taccaagaag gcggtggaat
    89401 cgggatccag ctatttaact cgatttattt gcttttgatc acgatgatca tcagttttcc
    89461 tatttcttta ggtgcgggta tctatctttc agaatatgct ggaaaaaact ggctaacaga
    89521 tgtgattcgc acgtcgattg aaatcttgag ctcgttgcct tctgtcgttg tcggtttatt
    89581 cggtttcttg gttttcgtta ttcaattcca gtatggtttc tcgattattt ctggtgcatt
    89641 ggctttgact gtattcaact tgcctctttt gactagaaca gttgaggagt ctttacaagc
    89701 ggtccattac acacaacgag aagcaggatt ggccttaggt ttgtcacgct gggaaacagt
    89761 gataaaagtg gtggttccag aagcgacacc agggattttg actggtgtga tcctaagttc
    89821 tggaagaatt tttggtgaag cagctgcatt gatctatacg gctggacaaa gtgcacctgc
    89881 gttagatttt acgaactgga atccattaag tgtttcaagc ccgatcagca ttttccgtca
    89941 agctgaaaca ttagctgtcc atatttggaa gatcaataca gaaggcacga tgcctgatgg
    90001 tgtttctgtt tcggctggcg cttctgccgt gttgattatt gctgttttac tgttcaactt
    90061 cggcgctcgt gcgattggca aacgattata ccgaaaaatg acttcagcct aaggagaaga
    90121 agcaaaatga aaaaatataa ctggaatgaa acaaatgtca tcaccatgcc aaaagagaaa
    90181 gaagtagcac tatatacgga tgatctacat gtctggtatg gcgacaatga agcaatcaaa
    90241 ggagtggatc tggaatttga aaaaaataag atcactgcac tgattggtcc ttccggctgc
    90301 ggaaaatcta cgtatcttcg ttcgttgaat cgaatgaatg atgggatcgc taatacgaaa
    90361 atcactggga aaatcatgta tcaaggcgta gatattaatg cgcctcaagt ggatgtttat
    90421 gagatgcgca aacggattgg catggttttt caacgtccta atccgttcag caagtcgatt
    90481 tatgacaata ttacatttgc tttgaaacag cacggcgaaa aaaacaaaaa gaaattagat
    90541 gaaatcgttg aaacaagctt gaaacaagct gccttgtggg accaagtaaa agacagcttg
    90601 gacaaaagtg ccttggcttt atctggcggg caacagcaac gtttatgtat tgctcgtgcg
    90661 atcgcgatga aacctgatat cttgctgcta gacgagccgg caagtgcttt ggaccctatt
    90721 tcgacaggaa cggttgaaga aacacttgta aacttgaaag aagactatac gatcatcatc
    90781 gtgacgcata acatgcaaca agcagctcgt atcagcgact atactgcctt tttctattta
    90841 ggaaaagtaa tggaatatga caaaactaac aagatattta ccagaccaaa aattcaggca
    90901 accgaagatt atgtgtccgg tcattttggt taagacggag gcctcaacat gacaaaagag
    90961 attatttcat ctaaagatct tcatctgtat tatggaaaga aagaagcact aaaagggatc
    91021 gatctaagct tcaatcaagg ggaaatcact gcgatgatcg gtccttctgg ttgcggaaaa
    91081 tccacttatc tgcgttcttt gaaccggatg aatgacctga ttccgaatgt aacgatcacc
    91141 ggaagtgttt tatataaaga caaagacatc tatggtccaa aaacagatgt tgttgaacta
    91201 agaaaagaaa tcggcatggt attccagcag ccaaacccat ttcctttttc tatctatgaa
    91261 aatgtcatct atggattgaa attaaaaggg gaaaaagata aaaaagtcct tgatcaagta
    91321 gttgaagaaa gcctgaaggc agcgtctgtt tgggatgatg tgaaagacaa attgcataaa
    91381 agtgctttat ctttatccgg tgggcagcag cagcgtgtat gtatcgcacg tgttttggca
    91441 gttaatccgg aaatcatctt attggatgaa ccaacaagtg cgcttgatcc tgtttctact
    91501 gggaaaatcg aaagtatgct gttagaatta agagagcggt atacgatgat catcgtgacc
    91561 cataatatgt ctcaagcttc acgtatttca gataagacgg cattcttttt agatggtcac
    91621 ttgatcgaat acaatgatac gaagaagata ttcatgaatc cagaaaaaca agagacagaa
    91681 gattatattt caggtcgatt tggataagga ggaaacggaa tgctacgtac acaatttgaa
    91741 gaagaattgc tgaatctcca taatcagttc tacgaaatgg ggatgatggt gagcagcgcc
    91801 gtgcacaagt cagttcgtgc atacatcaaa cacgataaaa tcttagccca agaagttatc
    91861 gaaaacgata taaatatcaa taacatggaa acacgcttgg agaagaaaag ttttgaaatg
    91921 atcgcattgc aacagccagt tacaactgat ttgcgtatga tcattacggt catgaaagct
    91981 agctcggatt tagaacgtat ggccgatcat gcggtgtctg ttgctaaatc cacgattcgc
    92041 gtaaaagggc agacgagaat accggaaatc gaaaaagaga tctccgatat gtccgattat
    92101 gtgaaaaaaa tggtggacaa cgtcctagtt gcttatgtca aaacagacga aaaagacgca
    92161 cgtacgatcg ccaatatgga tcaaagagtc aatgactatt tcaatcgaat ctataatgca
    92221 acaatcaaaa atatgcagga aaatcctgag acagtcatca gtggaacaga ctacttgaat
    92281 gttgccagtt atctagaacg gatcggagac tatgtgacaa acatctgtga atggatcgtc
    92341 tacttggcta cagggaaaat cactgagtta aatacgaacc acaatgagat tttctaaact
    92401 gaagctgtga ttatgccgtt tgtttaggaa aagaggttgt gaaatcagcg gttgtgaaaa
    92461 agctgttctg catcaagtaa taagaacagt caaacaaaaa atagctcctc atatttttcg
    92521 tttggctgga cttattaccg cagatgcaag cttttgaaca ccgtttattc gggaaagggt
    92581 ttgtgacaaa acatgtcaca aacccttt
//
LOCUS       DAFEOM010000006        73465 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.006, whole genome shotgun sequence.
ACCESSION   DAFEOM010000006
VERSION     DAFEOM010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 73465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.006"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            <1..1087
                     /locus_tag="LP524_000487"
     CDS             <1..1087
                     /locus_tag="LP524_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL2233566.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1308..2450)
                     /locus_tag="LP524_000488"
     CDS             complement(1308..2450)
                     /locus_tag="LP524_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL2233567.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGICLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2455..2640)
                     /locus_tag="LP524_000489"
     CDS             complement(2455..2640)
                     /locus_tag="LP524_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBL2233568.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2994..3239)
                     /locus_tag="LP524_000490"
     CDS             complement(2994..3239)
                     /locus_tag="LP524_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2233569.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3233..3634)
                     /locus_tag="LP524_000491"
     CDS             complement(3233..3634)
                     /locus_tag="LP524_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL2233570.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3819..5627)
                     /gene="uidA"
                     /locus_tag="LP524_000492"
     CDS             complement(3819..5627)
                     /gene="uidA"
                     /locus_tag="LP524_000492"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HBL2233571.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KLITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWKERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLQKRWSSIPNFSYKK"
     gene            complement(5650..7416)
                     /locus_tag="LP524_000493"
     CDS             complement(5650..7416)
                     /locus_tag="LP524_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HBL2233572.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IISDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIEPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(7429..8223)
                     /locus_tag="LP524_000494"
     CDS             complement(7429..8223)
                     /locus_tag="LP524_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBL2233573.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYYQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGEYVDIEQKFIDTFWKNLTNK"
     gene            complement(8233..9609)
                     /locus_tag="LP524_000495"
     CDS             complement(8233..9609)
                     /locus_tag="LP524_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL2233574.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLIIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQFKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKTSNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANIQTGDVLEKLYNASTLIPSICFFIVGLILFFLYPLSKDKVLENC
                     ELLKQKYK"
     gene            complement(9729..10376)
                     /locus_tag="LP524_000496"
     CDS             complement(9729..10376)
                     /locus_tag="LP524_000496"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HBL2233575.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSLENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(10469..11896)
                     /gene="uxaC"
                     /locus_tag="LP524_000497"
     CDS             complement(10469..11896)
                     /gene="uxaC"
                     /locus_tag="LP524_000497"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBL2233576.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLGYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(11970..13049)
                     /gene="uxuA"
                     /locus_tag="LP524_000498"
     CDS             complement(11970..13049)
                     /gene="uxuA"
                     /locus_tag="LP524_000498"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBL2233577.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLQTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(13052..14683)
                     /locus_tag="LP524_000499"
     CDS             complement(13052..14683)
                     /locus_tag="LP524_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBL2233578.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSNIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            14989..15660
                     /locus_tag="LP524_000500"
     CDS             14989..15660
                     /locus_tag="LP524_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL2233579.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQHGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKINNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKSI"
     gene            complement(16363..16718)
                     /locus_tag="LP524_000501"
                     /pseudo
     CDS             complement(16363..16718)
                     /locus_tag="LP524_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16735..16902)
                     /locus_tag="LP524_000502"
     CDS             complement(16735..16902)
                     /locus_tag="LP524_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002404637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233580.1"
                     /translation="MQTLINALQGKNYDYAVKILLDVAKRSLTPAQFAKFNAATIAAG
                     IAINAISCWGK"
     gene            complement(16902..17309)
                     /locus_tag="LP524_000503"
     CDS             complement(16902..17309)
                     /locus_tag="LP524_000503"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233581.1"
                     /translation="MSRFFKFFSLLGVMLIGLSTLVGAGTSVYAAEKVNEVPDIETQR
                     QQAVESLKFYLEEAGYVDTETHQYIVTDLEAIQKKAELDGSEGIAGKFILENFVQPTG
                     KRQKRDLQKYAACVVMNSLPFGVLFGIYLIVKI"
     gene            complement(17810..18004)
                     /locus_tag="LP524_000504"
     CDS             complement(17810..18004)
                     /locus_tag="LP524_000504"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233582.1"
                     /translation="MKDQKTNNKTGERSACSWAFTGAGLLHSGIYTAAALGLSLTGVG
                     AAVIPFAVGTAYGIAGAAIC"
     gene            complement(18018..18356)
                     /locus_tag="LP524_000505"
     CDS             complement(18018..18356)
                     /locus_tag="LP524_000505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233583.1"
                     /translation="MKRIVKIFGILGVFLIGLSTFVSAGISVDASETNNFLVKQQETE
                     INIIQNNLATHKDSKGNRLVYIIDESDLKSSLDRINSEITVSEIQQYLNEFNRSIQKK
                     WPWMANRSAS"
     gene            complement(18400..18732)
                     /locus_tag="LP524_000506"
     CDS             complement(18400..18732)
                     /locus_tag="LP524_000506"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233584.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRKEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLVWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(18746..19003)
                     /locus_tag="LP524_000507"
     CDS             complement(18746..19003)
                     /locus_tag="LP524_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233585.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KVYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(19198..19734)
                     /locus_tag="LP524_000508"
     CDS             complement(19198..19734)
                     /locus_tag="LP524_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233586.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLTTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDYMT
                     DRCIKDNSIDVDPHVQMKTTGKLHQVTQDIENPDQYLLFGEETTNEVTQFVLLQLKVD
                     QEAQKIDAIKIYYVRSVY"
     gene            complement(19755..20846)
                     /locus_tag="LP524_000509"
     CDS             complement(19755..20846)
                     /locus_tag="LP524_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBL2233587.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEDGGEATGLAVAFNWE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESTYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKDCYDYYGFLVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYQGAMSIIKSYNLTEYDTFAIKHWNSLPTKK
                     SSLVAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YDWTAYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSDGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(20854..23064)
                     /locus_tag="LP524_000510"
     CDS             complement(20854..23064)
                     /locus_tag="LP524_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233588.1"
                     /translation="MAYIKRKEKKWCVWLFLTVGFGLFFVGGLVYAVEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYLLLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPAFDYMIMNTLKSFLSLMFQKSFYSVIILVAFLIY
                     NIVDDLILMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGGQVINPFELGGRSNQDLPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQSSKQQGNNQRKPLLNKGIAFVGQSIPLPAGKVPVPQLTAAGSLS
                     QQIHQVKNQAKNGSYPAMQQLLNQYSVPKSSVATLSGNRSVQRQIQRSSESKPSISVP
                     KSLQPAFSSFNNPVEQSKKIEKKNETLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(23080..25608)
                     /locus_tag="LP524_000511"
     CDS             complement(23080..25608)
                     /locus_tag="LP524_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL2233589.1"
                     /translation="MVLVKEPVKALKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFYDVLNLIWLDVAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRMPKEHKGLLQLEDELGTSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEYKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATIIQQLHTTIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAIGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVEYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILTHLRLEVNEANLSEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQIEEQDTLIMQ"
     gene            complement(25662..26072)
                     /locus_tag="LP524_000512"
     CDS             complement(25662..26072)
                     /locus_tag="LP524_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233590.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFFIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYVV
                     RMMNGDCVYYNWDKVSNNALEELLFANKYVYLED"
     gene            complement(26085..26303)
                     /locus_tag="LP524_000513"
     CDS             complement(26085..26303)
                     /locus_tag="LP524_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233591.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(26300..27292)
                     /locus_tag="LP524_000514"
     CDS             complement(26300..27292)
                     /locus_tag="LP524_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL2233592.1"
                     /translation="MKKRQLRRKEKVYTEKVPNQRKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSQSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQEILKKEFYAQNFIQEPGSVSGIRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMDPENKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKTFIVKATVVFRDGDTSLLQKEQMTLVITKKTNQYYVEKLTHNWLDRKGDERK"
     gene            complement(27371..28282)
                     /locus_tag="LP524_000515"
     CDS             complement(27371..28282)
                     /locus_tag="LP524_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL2233593.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLSDVETELNGGTTEKPAEPEHLQPEKPEKEPVAPEPEIPESTLPTDPIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSETNTDSSSTEQPTMSFTSEEPKEEPKQPAEKSEAIPK
                     ENKPIVPAKEVTVSVTPNGEITTNNSQGMSVPIVTSNVEELTHIPTPTTPLKAEGGQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEVKTADGKTKVLPKTGEEIHVVLS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(28301..28645)
                     /locus_tag="LP524_000516"
     CDS             complement(28301..28645)
                     /locus_tag="LP524_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233594.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLGPRTERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(28665..29222)
                     /locus_tag="LP524_000517"
     CDS             complement(28665..29222)
                     /locus_tag="LP524_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233595.1"
                     /translation="MSLEFLGEPIEKEEFVSHYMDLFIRVIKKLCTIDEFYPREQDYL
                     AAERIKISLLWEKFTDENNRPPDYRYLSDCLTNNVLSKEYLFHKREKNQMSAEHFCDC
                     YIALLRSQQLLTVERYGAEFGYVMQAERHRATRCYEKEDQVIDGYEELCIRNNRYLQQ
                     RLYEKLMMDFSDLYRGYECSVIQER"
     gene            complement(29302..29802)
                     /locus_tag="LP524_000518"
     CDS             complement(29302..29802)
                     /locus_tag="LP524_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233596.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPKEYPIEKRELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPINFLKKC
                     HLRILP"
     gene            complement(29803..30348)
                     /locus_tag="LP524_000519"
     CDS             complement(29803..30348)
                     /locus_tag="LP524_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233597.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGTLKFIRFYRFSLPVPAILAVK
                     KGCDKMKIYENCVAALSNEKPLVEEISLATVTWEMNRQPTEEQTYLSLEEIELELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(30345..31601)
                     /locus_tag="LP524_000520"
     CDS             complement(30345..31601)
                     /locus_tag="LP524_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBL2233598.1"
                     /translation="MTPIELRNYRKKLSAIHASGGGKKLLSQKRFAQLLGCSVDYIKK
                     MEQGKKKIPEDFEKRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKIVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKQTVTQNVLVQFSGQGCREYEEVMREQRTD
                     WRTWLYSIWKNQGKLEAGYKRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKKFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISEIEAL
                     TKYGIYNRYELRLANEKATQAVEAFILDHSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDERWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIENAELNDEQQSYLEWMGTSGGKIA"
     gene            complement(31964..32278)
                     /locus_tag="LP524_000521"
     CDS             complement(31964..32278)
                     /locus_tag="LP524_000521"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233599.1"
                     /translation="MGEVLYRVSSAAGEISPDFAVRRLYEWINKVEYYTKGSYVFRRI
                     ERETLFVTRNQIVLTKEDILRFRQVYRLCKEKNIQLRLAILQCFAPEQYKELQEKEDS
                     LV"
     gene            complement(32405..33598)
                     /locus_tag="LP524_000522"
                     /pseudo
     CDS             complement(32405..33598)
                     /locus_tag="LP524_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
     gene            complement(33788..34144)
                     /locus_tag="LP524_000523"
     CDS             complement(33788..34144)
                     /locus_tag="LP524_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233600.1"
                     /translation="MNLDKTNQTLREIMVCCQNIQQLIEYFGDNPTIFIQTNEYICAV
                     IGQCMFLRDLVRFSLPYQWKQDHQEVVWIRVVRICNMCAHPYKNRLNLFRLWFFIKCD
                     IPVLVAFCQKFLAQEK"
     gene            complement(34210..34662)
                     /locus_tag="LP524_000524"
     CDS             complement(34210..34662)
                     /locus_tag="LP524_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233601.1"
                     /translation="MGLKFPNNYDITEALDETCIGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNSNPKQSQEKGLDQKQPSADSKEKEKK"
     gene            complement(34664..35029)
                     /locus_tag="LP524_000525"
     CDS             complement(34664..35029)
                     /locus_tag="LP524_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233602.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIEEVL"
     gene            complement(35046..35240)
                     /locus_tag="LP524_000526"
     CDS             complement(35046..35240)
                     /locus_tag="LP524_000526"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233603.1"
                     /translation="MEKKNINICFLSGKECTYEECTDISVHERDGMLIVDMSPSDEPF
                     VVKHYFPLCNIESFRIDCEF"
     gene            complement(35257..35502)
                     /locus_tag="LP524_000527"
     CDS             complement(35257..35502)
                     /locus_tag="LP524_000527"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233604.1"
                     /translation="MEEIFFCMMVEFLFVSIGFLIMGEAIMIYLFPFDSKDECIFSSI
                     IIFFILACVWILAREIYHQFVLEDGKGIITWFLQKRK"
     gene            complement(36218..40513)
                     /locus_tag="LP524_000528"
     CDS             complement(36218..40513)
                     /locus_tag="LP524_000528"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HBL2233605.1"
                     /translation="MYAKTKMNWLKALALVALSQFCFLVGTAQAHAEETTATQPTVEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPVT
                     SQPVEETASITTPASETSTPEGEKAAEATPSVKPESSEKQAEEKQRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKLNKESTASIEDQKKKEQDIPKTGTPTLLVTVP
                     TNRSVRVRRATNTAVLGGRKIDWGENIGVTSSPNNPNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALENYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLLNNFDVRATFHNQTHTIKIGETLKVKSGNDFLQRV
                     KGIQKPKGINVSIHGNEAWITVTKEAPEKAKIHFNQIEVLGTPLAYRRPGAQTIGILN
                     PFEPGDSVVNLTILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KNMNGKVQAEIKDLDPGKYVLKEIKAPKGYALSDETIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKIANKDLVDSTNPDNKPKLAGIEISLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKERYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVT
                     FTLYNGKGQAIGKYTTDQNGEILVKGLVANNYYFTENVPLESFQPNTGKQAFTITFRQ
                     NAPDDGETLLVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQEGKEITSILTFIADKANGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAVHVDINDDNQSVRVNNPTVHTTAVFEDGLTIADPTGLFTLNDTVEINE
                     ILPGKAYLVKGILMDKATGEALLVHGQPVESAVGFIAKAENETVIVPFTFDLTGLNGR
                     EMVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLIVGKTYTVNGVLMDKVTEKPVLVDGKEVSSTLTFVAETTDGTVNLDFILD
                     AHALRSKEVVVFEDLFREGVLLASHADITDKDQTIKIVSPTITTKATNANGGKEILAL
                     PNQKVVEWVQVNGLAIGQIYKLIVQGYLTPDGTPFEGTHAEKVFMADKEMMEFLFEFI
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQFVEAPKPPQPVK
                     PNEPQPVKEVKQAAMVPMLPKTGSEDNVLWIMIGMIFVLAAGLIGYDLYKVRD"
     gene            complement(40660..40899)
                     /locus_tag="LP524_000529"
     CDS             complement(40660..40899)
                     /locus_tag="LP524_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233606.1"
                     /translation="MIDISIPEDLKPDPATIGNSGMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMIFGDGEVANKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(40989..41261)
                     /locus_tag="LP524_000530"
     CDS             complement(40989..41261)
                     /locus_tag="LP524_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233607.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            complement(41344..41595)
                     /locus_tag="LP524_000531"
     CDS             complement(41344..41595)
                     /locus_tag="LP524_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233608.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFVESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            complement(42593..43504)
                     /locus_tag="LP524_000532"
     CDS             complement(42593..43504)
                     /locus_tag="LP524_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2233609.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGQLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEISKESNRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFNKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            complement(43701..45536)
                     /gene="lepA"
                     /locus_tag="LP524_000533"
     CDS             complement(43701..45536)
                     /gene="lepA"
                     /locus_tag="LP524_000533"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBL2233610.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            45683..46903
                     /locus_tag="LP524_000534"
     CDS             45683..46903
                     /locus_tag="LP524_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2233611.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            46923..47417
                     /locus_tag="LP524_000535"
     CDS             46923..47417
                     /locus_tag="LP524_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBL2233612.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            47395..48081
                     /locus_tag="LP524_000536"
     CDS             47395..48081
                     /locus_tag="LP524_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233613.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(48232..50841)
                     /gene="clpB"
                     /locus_tag="LP524_000537"
     CDS             complement(48232..50841)
                     /gene="clpB"
                     /locus_tag="LP524_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBL2233614.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(51092..52138)
                     /locus_tag="LP524_000538"
     CDS             complement(51092..52138)
                     /locus_tag="LP524_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBL2233615.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            complement(52149..52421)
                     /locus_tag="LP524_000539"
     CDS             complement(52149..52421)
                     /locus_tag="LP524_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBL2233616.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            complement(52456..53364)
                     /locus_tag="LP524_000540"
     CDS             complement(52456..53364)
                     /locus_tag="LP524_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBL2233617.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            complement(53496..54728)
                     /gene="pepT"
                     /locus_tag="LP524_000541"
     CDS             complement(53496..54728)
                     /gene="pepT"
                     /locus_tag="LP524_000541"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBL2233618.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            complement(54743..55864)
                     /locus_tag="LP524_000542"
     CDS             complement(54743..55864)
                     /locus_tag="LP524_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBL2233619.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            complement(55861..56562)
                     /locus_tag="LP524_000543"
     CDS             complement(55861..56562)
                     /locus_tag="LP524_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HBL2233620.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            complement(56706..57146)
                     /locus_tag="LP524_000544"
     CDS             complement(56706..57146)
                     /locus_tag="LP524_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBL2233621.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            complement(57275..58201)
                     /gene="dnaI"
                     /locus_tag="LP524_000545"
     CDS             complement(57275..58201)
                     /gene="dnaI"
                     /locus_tag="LP524_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HBL2233622.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            complement(58201..59574)
                     /locus_tag="LP524_000546"
     CDS             complement(58201..59574)
                     /locus_tag="LP524_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBL2233623.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            complement(59597..60088)
                     /gene="nrdR"
                     /locus_tag="LP524_000547"
     CDS             complement(59597..60088)
                     /gene="nrdR"
                     /locus_tag="LP524_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBL2233624.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            complement(60400..60732)
                     /locus_tag="LP524_000548"
     CDS             complement(60400..60732)
                     /locus_tag="LP524_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBL2233625.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
     gene            complement(60784..61413)
                     /locus_tag="LP524_000549"
     CDS             complement(60784..61413)
                     /locus_tag="LP524_000549"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBL2233626.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            complement(61377..62213)
                     /gene="mutM"
                     /locus_tag="LP524_000550"
     CDS             complement(61377..62213)
                     /gene="mutM"
                     /locus_tag="LP524_000550"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HBL2233627.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            complement(62258..64903)
                     /gene="polA"
                     /locus_tag="LP524_000551"
     CDS             complement(62258..64903)
                     /gene="polA"
                     /locus_tag="LP524_000551"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBL2233628.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            complement(65029..65712)
                     /locus_tag="LP524_000552"
     CDS             complement(65029..65712)
                     /locus_tag="LP524_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBL2233629.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            complement(65705..66190)
                     /locus_tag="LP524_000553"
     CDS             complement(65705..66190)
                     /locus_tag="LP524_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HBL2233630.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            complement(66497..67831)
                     /locus_tag="LP524_000554"
     CDS             complement(66497..67831)
                     /locus_tag="LP524_000554"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBL2233631.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            complement(67962..68723)
                     /locus_tag="LP524_000555"
     CDS             complement(67962..68723)
                     /locus_tag="LP524_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBL2233632.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            complement(68739..69221)
                     /locus_tag="LP524_000556"
     CDS             complement(68739..69221)
                     /locus_tag="LP524_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HBL2233633.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            complement(69241..71421)
                     /locus_tag="LP524_000557"
     CDS             complement(69241..71421)
                     /locus_tag="LP524_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBL2233634.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            complement(71565..72296)
                     /locus_tag="LP524_000558"
     CDS             complement(71565..72296)
                     /locus_tag="LP524_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HBL2233635.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            complement(72365..73306)
                     /locus_tag="LP524_000559"
     CDS             complement(72365..73306)
                     /locus_tag="LP524_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBL2233636.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
BASE COUNT        21568 a        15400 c        11398 g        25099 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaaaa
     1261 aaaaaaatag gctgtgacag cagcgtgaca gcaaaaaaga tggaaagtta aatatcaaac
     1321 ctcgtaattt catttgcgag tgcttgattt gtttctggat ataagtgtcc atatttgtcc
     1381 aaagtagttt taatagaggc atgacctaga cgcttagata ttgcatagat atttttatta
     1441 tgttcaatta aaaaggcaac atgcgagtgt cttaatccat gcatatgaat tggttttact
     1501 cctgcaattt cagctaattt ttttattctg cttaaaatag ttcttggaga tggcggtaat
     1561 ccatcaaaag aaaaaaccaa ttctatattt ttcccaattg attgttgagt ttttttccat
     1621 gtttttaaat cttcaacggt ttgtctgtct aacgaaatta tccgaatact tgatgtattt
     1681 tttgtttcag caaattcata atcttgtctt gtacggatgt aaattgactt atttacgcta
     1741 catctgccat ttattaagtc aaagtctttc cattgtaacg ctaagagctc ctcactcctt
     1801 acacctgtaa caaaaataaa acgcatcaaa cgtttataaa attcttcata aaaatccccg
     1861 tgataaacac tattatatac tttcttaaat tcatcaactg tccaaaattc tacttgagga
     1921 cgctgagttt ttattttacc tatagtatct acaggatttt cttcaataat ttcaagtaca
     1981 attgcccttt taaaaactac tctcaactta ttgaatatct gtttaatata atttggtgat
     2041 aatggttgct caatgccctt ttttacaaca tggattttcg ataaaaactg ttgaaattcc
     2101 tgtatatgaa ttggtcgaat ctttacaacc tgcatttctc caaaaaaatc caatgcacgt
     2161 tgtaaaatat gatctgtttt catatatgtt ttttctacag ttcccaactt ataccaagga
     2221 aaaaaatact ctcgataaaa atcatgaaac gttattcctt gcagacaaat tccttgtgaa
     2281 tcagaaaaac tttgttttaa ctcttcgagt ccccttctag cttctccttg agttttgaag
     2341 cctcttctgg tcacttgaat tctttttcca aagcgatctt gtcctaaagt tgcagaaaaa
     2401 aaccaagtgc ctctttttct gtctttataa acattcgcaa ttttcctcat tttactattc
     2461 ctcccaagaa atatggagaa cttcttctaa aattgaaata ggaattcttt ttatttttga
     2521 attacgataa aaaaagtatc ctttctcaac taaaagttct ttacattcct tgaagatttt
     2581 attagcatag tactgagaaa aacctagatt aatcaaatct tttcgagtag ctgttttcat
     2641 tatagcacct tccttacttt ttttcattta tttctatcag ctgcttttgt caaaagcaca
     2701 taggaatttc acctctcccc aagtttacgg cgagccttac ggaagtatca ttatcccaac
     2761 tttaaatcat cgctttttat tgactataaa aattatttta gaatatttat cgctccttct
     2821 ttacagcaag aaatcatggc gcaactaaaa tatgctcctt aaagtgttaa cgtactgata
     2881 gaatctattt aattttcaat gaacaatagg ttttctacct aaaagaatct actaattcct
     2941 taactattag aagattcttt taaacagaaa aagtggaaag gagatttttt taattagtta
     3001 attttaaatt ttaagatcgc ttctattaat ttaatttcta gctgacttat catatattca
     3061 tctacatagg taacgaattg acctacctcg ttaaatccat ttgtcaaaca taacttagcc
     3121 atataactat gataatgttt gagtatcatc ttgatcgaaa ttggatctcc ttctagagca
     3181 gataagataa tggatagtgg aactaatagg tactgccgct ttttggattt atctaccata
     3241 ggtcataccc cattttcaat tgttcaactg ccttctttcg ttgataccag ataccacttg
     3301 tgctcatatt gagaatttta ccgatttcag catcattaaa acctgcaaaa taataaagta
     3361 ggataatctt tcttcttatc tccgataact gatcaattgc tgctgataac ttttgatctg
     3421 aaattaatat ttccattcca aataagaaat agacatcttg ttcatcaaaa tgagtttggg
     3481 aatacctgcc ttcatttgaa tctgccaatg gttcctcatt atcctttatg ttagcatttc
     3541 gttttcgaat agacttagct tcgtttcgta atactttttt acagtaggca tcaaattggt
     3601 gttgtataga tatgtcattg taatgttgtt gcatgataat atgctccttt ttcccccttt
     3661 ccactaatac aacaatcaga gagccccttt ttttcgaaaa aactagaaaa aaacataaaa
     3721 atatttatca ttctttaaac agaaaatctt tctataaaaa acaaaaaaac gattaggcta
     3781 ggctaatcgt tttttgaaca ttcttaaaaa aatccaaatt attttttata actgaaattg
     3841 ggtattgatg accatcgttt ctgtagatag tgagcaatca ttttaggttt tctatctcta
     3901 gtaaagattc ctttcttatt tccttgcact cttaaaatcc cttgactcgt tgcaaaatct
     3961 gcaaaattcc acacttgttc accaacaaaa ttttcacatg tatctattac ttcgtgattt
     4021 gcttcgtaat aagccacttg atactcttct gtaaacattg ttggtactgt atcatgaaat
     4081 ccagctactg tatctgctcc atattctgta aacattattg gcgtatcagg acatctttct
     4141 ttccatccat ctagctcttt tttcaacaga tattttgcat gtgccagatc ccctcccgaa
     4201 acataccaac cataataacg atttaaacat aaaacatcga caatatctcc taccttacaa
     4261 atatctggcg ttgattttag ataagtaact attgttacag gtcttttctg cggatcaagt
     4321 tctttagtta attcaactaa aggttcaaaa tattctttag caccttcgga atccgaatct
     4381 ggttcatttg caatagacca cattacaaca cttgggtggt tcttgtcgcg ttctatcaat
     4441 tccttgatca ccgttttatg agaattttct gttcgtatct ctttccacgt attatgtttt
     4501 tccacattat catctaataa agtggccatg aaatttaaat gtaaccctac tgcaggtact
     4561 tcatcaataa ctacaatacc ttctcgatct gctaatcgca taatttcttc tgagtaagga
     4621 taatgtgatg ttctaaaaga attagcacct atccatttca ttaaattaaa atctttaata
     4681 tttacagcct cattcaaacc acgtccatta acatacgaat cctcatgctt accaaatcct
     4741 ttaaaataaa aaggtttatt attaatcaaa aatttgccat tggtaacctg aacactgcga
     4801 ataccaaatt cttctgtata ggtatcgatc actttattat catcaattaa actaattttc
     4861 atactatata aataagcatc tagaggttcc caaaaaattg gattcttaag ctgaatcatt
     4921 cctttctttc cttcagcttc agctacaagt tgagcctctt cgtcatatat cgctattaag
     4981 atttttccgg tccattctgt atcgatattg aattctacac ttgcatccaa atcagttatt
     5041 cttgtattaa tagtaatatc acttatatag tctttcggag ttgtatataa cttaacaggt
     5101 ctatgaatac ctgcataatt gaagaaatca aaattaacta agttttcctt ttttatatcc
     5161 ccattttcct gtactgtttc cttcataatc cccacaggta aagtactatc gtcaataata
     5221 ttgtttacag caactgtaat tcgattattc ccgtctttta caaaccgatt aatttcagac
     5281 tcaaatggtg taaacccacc tttgtgttga gtaatcaact ttccattcaa aaaaactttt
     5341 gcttcatgtg ttgcagatcc aaaacgaaga actaatcttt cttctaataa tcttttagga
     5401 agagtcactt ctttttcata ccacacccat ccaacatgat ttctaatgtt ctctgtttca
     5461 aataaatcat tataagaaga gggaactgaa atcggataac tctctttttc atttattggt
     5521 tctaaagcaa gttcttcctg taagtcatca tttgtatcta atttaaatct ccagagaccg
     5581 cttaagtcaa ttacttgcct cgtctctgtc ataatgggat acaacatata aaccactcct
     5641 atctaaatat taaataaatg tatcctttaa accacaataa gcatctattg gacttactcc
     5701 cttaaattca gaaagaccca ttagtttgtc caccaatatt tctaaagttt cttgttttcc
     5761 atcataagta ttaatataag tctttacttg aggtacatca gccaaatgaa acggacactg
     5821 tacagataca aaaattgttg gaatttcatg aatgtaaaac ggtatatccg gagttccttt
     5881 acttgctggc cagacaattc gttgaactgt gcctggatta acattagcta tattaatgat
     5941 caagtcatat tttttagtta aatttttaat tggtctctta gcagcatata ttccagctat
     6001 atttttaatt ttttcttctt gcgataatcc tttcaactct tcttcaggtg aaatgtattt
     6061 ttcaacttca aatccttttg ctgacaaaat ctgtttcaat aaatcaatgg cttgaggtct
     6121 atttccagca actacttcac caaacccacc ttttacccca tgtacatcta ctagtaaaat
     6181 ccttttatat ttttctggtt caataggcca aatatcttgt ttatctttaa ctaatgtaat
     6241 tgcttggtca gccacctctt taaaaattga tttattttca attaaaccaa ttttttccag
     6301 agcttcttgc ttaggtaata aaatttgttc ttttgctttt ttgtgtaatc ccaatttagc
     6361 ttttaaccct aaaattcttt ttaaggcatc taataaccga tcatcagaga taattccttt
     6421 acgataacct tccatcatat aattaaagtc ttcatctgga tcattaaaaa atagaaatag
     6481 atcacatcca gctgcaatag aagtgggcaa taattctgat cgtttcattg ccccagtcat
     6541 tcctaacata tgactagcat cagtaactac taatccatta aatccgagtt ttcctctaag
     6601 taagtcagtg attaattctt ttgataaagt tgcaggaagt aaatcttcat ctttcagatc
     6661 aggattaaaa tgttttgaat aagctggcat atggatatga cctgccataa tcgaaggtaa
     6721 tccagcttct attaaacctg agtatacttt tccaaaagaa ttatcccatt cttccgtacc
     6781 taaactatta atactaaaag atagatgctg atctcgttcg tcaataccgt caccagggaa
     6841 atgtttagct gcaggcaaaa ttccactctc ctgaatacct ttcatataag ctaaactaag
     6901 ttctaaaact ttatcagggt tttgtccaaa agatcgacta gaaatgatag gatttctcca
     6961 atttctatta atatctacaa taggagcaaa actccagtta catccaatag ccgatgcttc
     7021 aatcccagaa actctcccta attcataagc atatttcgca tcatttgttg caccaatttt
     7081 tacttctaca ccaacttcag taccatctgt acaagctccg ttaccacctg cttccgtatt
     7141 agcagcaatt aataaaggaa ttttgctttc tgtttgtagg atatagtttt gatcatatac
     7201 ctcatcagca gttcctgggt tatatcgtac agctccaata tggtactgat tgaccatatt
     7261 actcaagtaa tttttatcac gactagatcc catattaaca aacaattgac ctattttttc
     7321 ttccaaagtc atagaatcaa tcgtctcttc aatccaagaa atagcatctt catctaaatt
     7381 atatggtttt tttcgtaagt ctacttttat agtcattttc tttctcctct acttattagt
     7441 aagatttttc caaaaagtat ctataaattt ttgttcaata tctacatact ctcccgataa
     7501 acatttaaat aaaacatcag ttcttaagtt ttcccatgat tctttttctt tattaaccaa
     7561 aataggataa aagaaaactt tatttttatt tgcagcttct agatcaccag gagaatcacc
     7621 aatcattaat atcttatcca aatcacaacc attttcaatt acacttcgaa taatgtcttc
     7681 tttttttcct ttatcttgac aacacagttc atcaactaat gataataatt catgtctttc
     7741 ccattcttct tgaacagctt ccttattagc tgagcttaca ataattatct tcccatattt
     7801 tttcagagct tttatagtat ctctagcact taaaaagggt ttatctaatc ctttagcttg
     7861 actaatctgt tgattgactt cttccgacca ttttaaagct tttaataaat cctctgactg
     7921 ataatattct atttccctct tcaaagatgg attagacaat tcttttgtta gagataccca
     7981 gtgctttaat cgattaatgg atgttacatc atttttttct aatgttttaa ctaaacctaa
     8041 aaatctgttt attccacgag tctctgaata taaattaata ttattccaat cctgtaggaa
     8101 tttttctcta tgatgaacat caaaaatctc tgcagcaatt ggtccgaaaa agagattgtg
     8161 tttataagtc atcgtatcca taacacagcc atctgaatca atacaaaata tccaattatt
     8221 catatttctc tcctatttat atttttgttt taaaagctcg caattttcta agactttatc
     8281 tttagataat ggataaagga aaaataaaat taatcctaca ataaaaaaac aaattgatgg
     8341 aattaatgtt gaagcgttat ataacttttc taaaacatct cccgtttgta tatttgctaa
     8401 agagtcatag cctataatag aaagagtcca acctccgacg cctccagcta gagcttgacc
     8461 tatttttcta gcaaaagaat ataccgcata tacggtccca tcttctcttt gatcagactt
     8521 tacttcttga tcatcaataa catcagtaat atttgcccaa ataacagtat taaacacacc
     8581 caaacccata tagccaaccg aactaagaat taagaataca tacatattac tagtttttat
     8641 caaaaataag atcaaatata ctattgctga aaagaacatt cctactgatg ccagttcttt
     8701 ttttccatat tttttactta aaaacaaagt taaaggtgaa acaactatta aaaccaaaat
     8761 aggattgata aaattcatta aaactattcc ctgtgcatca ttatagtaat caggaaatac
     8821 ataattattc atactcataa ttaataattg agcaaggagt aagaaaatag ccgcaccaat
     8881 tatagaaagt aatgccctat ttttaactaa cccttttaaa gattttaaca atgaaacttt
     8941 gaattgagat tggtcaattc ttactcgttc agtagttagt ttataacata ttaaataaca
     9001 aataagtgca catatcgaaa atattcccgc aacaattgta aaagttgatc caccacgaac
     9061 tatttgagcg cctgaatcat ctcttgtata aataaataat ggtgttaata caccaatgat
     9121 caaacttgct aaagtagctc caacatttcg gaatgtcgat agcattgttc tttcttctgt
     9181 aatagaagta atagctgatg ccattgatcc ataaggaata tttatagatg tgtaaaaaat
     9241 actcccccat aatatatacg taacatacat gtatactacc ttaacgctca tagaagctga
     9301 aatcatagaa gtttgataca ttaaaaaact tgctaaagcg actggaccac tcattcttaa
     9361 aatccacggt ttaaacttac cctgcttagt tgctttcgat ctatcaacaa tacgccccat
     9421 agttacatca gtaactgcat cgataaatct tgctactaaa aataatgttc caacggcagc
     9481 tccgcttatc cccaatactt ttgtataaaa gatcattaaa aaagaactag caaaaatgaa
     9541 agtaaaatcg tttccaaaat ctccaaacat atatcctatt ttgtctctta taccaaattt
     9601 tctttccata caattctctc ccttcttttg ttaagcgttt tcattcttat gaaagtacgt
     9661 actatactct gaagcgtaaa gagtcagtat ttatttttta catttcagag tattatttgt
     9721 tttttctcct aataattctt tatctcataa aacttatcta tatatttact agctactcga
     9781 cttacttctt caaaatcacc tttttcaaca gaagccaata aattcccacc tacaccaact
     9841 gcaacaactc ctacttcaaa ccaatcttct aagttctcta gacttactcc gccagtaggc
     9901 attatattta cgtgtggtaa tggagcttga taggctttta caatggatgg accaaaagca
     9961 gaacctggga atagtttaat gatatctacg ccagcagtaa gtgcctcctt tatctcagta
    10021 attgtcatac aacctggtaa ataaggaatt tgatataaat tacaaatctc agctgtttgt
    10081 cgatcaaaag atggactaac gatatattct gccccagcca taattgcgat acgagcagta
    10141 aacgcatcta aaaccgtacc ggctccgatt attacatctt tttgatcagc atatttttta
    10201 gcaatttctt tgattatttc ttcagcatta ggaactgtaa aagttagttc aatcccttta
    10261 attccacccg caataattga atttattgct ttaatggcct catccttatt tttcccacgt
    10321 acaaccgcaa tcactccagc cgcttctaac ttacttaaaa tttctacttt tttcatatct
    10381 atctcctcta tctccttctt ttatggatac atactttgtc tagctgtact aacttaaaaa
    10441 gaataaattt aagcttttat ttcaagtatt attttctact tccgatttca aaaaaattaa
    10501 aataattata cgcattacga taacaaatat tctccactat ctgactaaga tacatttcat
    10561 catctggaat tcggcctgct ttaacccact cgcttagtaa ttgacataat actcgacgaa
    10621 aatactcatg gcgaggatag cttagaaaac ttcgagaatc agtcaacata cctgtaaaat
    10681 tcgacaacaa actttgttgc gccatcgtag tcaactgttg acgcattcct tcagctgtat
    10741 cattgaacca ccaagcacat cccaattgga ttttttgaat cataccccct tggaagcttt
    10801 gcattgctgt tgctagtgga agccaatcag ttggatttaa tgaatataaa attgtttttg
    10861 gtaagcaccc actcttttca gcggtatcga acaatgctac aacattttgt gtcaaactag
    10921 cttgatcccc catcgaatca ccaccgacat ctactccaat ttgttccttt aatatacttg
    10981 aattatttct taaagcattt aaatgcattt gcatcgtcca accatatctt gtatactcag
    11041 acatcaaatg taattgaata cctgtaacaa attgtgcaat ttctgataaa tggaaatccg
    11101 tttttcctgc taaagctttt attaacagat tgtcgagtat tttttcatct gctatttcat
    11161 aaaaataagt attcaaaccg tgatctgcta atcgacagcc aatttgatga aaatgatcca
    11221 ctcgttggct caatgcttgc ttcaacccat taaagtcttt gattggtata gccgctgctt
    11281 gactaagttt taaaatatag ttcgaaaagc ctgatgattt gatactccag accttatctg
    11341 gacggaaagt aggtaatacc ttgaaagaat tatcctgttt agaaagctca gtatgatacc
    11401 ccaagtcatc tgtaggatca tccgttgtac agatggcttt cacatccatc atctccaaca
    11461 attctttcgg gcgataggaa tccgtttgga ttttttcgtt agcacgtcgc caaatttctg
    11521 gtgcattctt ctcagaaatt gtcaaatcaa tatcaaagta acgttgtaat tccaaatgag
    11581 accattcaaa aataggatta cctggcgcct tctccactgt ttttacaaaa gctaaaaatt
    11641 tttcataatc atcacctgga ccactgatca actcttcttc tataccatta gctcgcatca
    11701 atcgccattt ataatgatct ccagctcctt tatcatttaa ccatacctga gtcaaattct
    11761 tgtattgctt attttctagc aaatatgctg ggtctaaatg acaatgatag tcgataattg
    11821 gttggtgttt tgcaaattta tgaaaaagtc ttttagctat ttcattctct aataaaaagt
    11881 tcttatctaa aaacatattt aaacactcct ttccttccgt tagtttttag aagagaaaat
    11941 ttttcctcta aataaactcc attatataat tatttactcg cccccttttc aagcgcttcc
    12001 caaataccat ttaaataaac tgcacctaga gctcggtcat ataatccata accaggtcta
    12061 ccggtttctc cccaaatcat ccgaccatga tctggacgaa tatagccatt aaatccatta
    12121 tcgtacattg tttttaatat cttatacata tctagagaac catattctga tttatgcggc
    12181 gcttcgtaaa aatcgagtcc attgtcactt aaatatttga tattacgaac atgtgcaaaa
    12241 ggaatccgat cccgttgaca aaattcagat agaatttcat agacatcatt atctggtcgt
    12301 tctgcaatac ttccagtaca taatgtaata ccattagaag gagaatccac tacattacac
    12361 aaccaatcta aatcgtcacg atttttaaca atacgaggta atccgaaaat ggaatatggt
    12421 ggatcatctg ggtgaactgc cattttaatc ccaacttctt cacatgtcgg gataattgct
    12481 ttgagaaaat atgcaaagtt ctctcgtaat ttctcttcat caacttcttt ataggcttct
    12541 aataattgct taatttgtga taaccgatga ggttcccaac ccggcaattc aaaattatta
    12601 gtagaatttt ctacttttct aataatttcc tccggatcag tagggatatt ttttgaaata
    12661 taggctaacg tagaagaccc atcatctaaa cgataatcca aatccgattt tacccaatca
    12721 aatactggca taaaattata acagataacc tcaatcccaa attctgccaa attgcgaatc
    12781 gtctgtttat aattctcaat gtattgatct cgtgtttgta aaccaatttt gatatcatca
    12841 tgaacattaa cactctcaat aactttcaag gtaagtccat tggcttcgac ttcatctttt
    12901 aaagatttaa ttcgttctct cggccaaacc tctccggctg gtatatccat taatgttcca
    12961 acaattcctt tcattgctgg gatctgacga atttcttgta gtgagatttt ttcttcattt
    13021 gccccatacc atctaaaagt catttccata gttactttcc tccaacttct gttatatatt
    13081 tattgattgt tttacgaact gcttgaggac ctactagcat ttcgcaaaag attttttcag
    13141 ttttctttcc taccccaact tgatataaat ctaaaccaaa aatttcttta ttttctaaaa
    13201 tttcctgtac cgcacaatga atatttgaag gatcttgttc acctaatttc aatactgata
    13261 atttatcctg caactctacc aataacggat caggacttgg cacaaaattt tccccttgat
    13321 catcgactgc cagtaaataa cgtaaccaag cagcaatagc gataggaata aatgttagat
    13381 tttctgcatt tccgtctttt tcgacatact ttttaattgt ttcaccatag cgaatcgcta
    13441 ctttttgaga agtatctgtt gcaattcgtt gtggtgtatc tggaatatag ggattaggca
    13501 aacgtttatt aattacttca tcaataaatt gttttggatc aataatgcct ggattttcaa
    13561 ctactggtaa accttctaaa taacctattc ctttgattaa accaaccaaa tcatcatcct
    13621 gcatctctgc tgaaattgat tgatatccta gcagacaacc tgttactgcc aaagcagtat
    13681 gaagtggatt caagcttgtc gtaaccttca tgatgtctgc tttatcaacc gtctctctat
    13741 ctgtcataat cacaccagcc ttttccaatt taggccggcc atttggaaaa ctatcttcaa
    13801 taactaaata atgaactacc tcagtattag tgaaagcagc aatattggta tgtttttttg
    13861 tgtgaatgat atctaaatcc tcgagcccca tcatcaatag atcttccttt acattctctg
    13921 aaggatttgg cgtaattcga tcaatcatac tccaaggaaa agtcactttt tctttatcct
    13981 ttaaataagc aataaatttt tcagaaacat atccttcttt ttgccaagct tctgcaatcg
    14041 taaaaatact atcttgaaaa cgttgaccat tctgtgagaa attatcagta gaaaccatcg
    14101 caatcggcaa ctctccagca ttaaatctgc tatataacaa ggatgttaca atactcatcg
    14161 tatgttgagg attgctaggt ccagatttga tatcagcttt gactaccgaa aagaattgcc
    14221 ctttagaatc ttttaaactg taaccttttt cagtaatagt aaacgttgta aactgtaatt
    14281 taggatcttc aaaatattta accactcttt gatagtccgc tatattcttc ggattgcaat
    14341 ataaactatc agccgttact gccaaaagcc tttttttgag ttcaccatct ccactcataa
    14401 caatttccaa aatgtcattg ttgtagtcat gataagcttt ttccactact tcttcatcaa
    14461 atgtttcaca cacaactact cctgtttcta attcattttg atctgctaag cattgagcaa
    14521 tttctgcatg aaatcctcga taaagatttc ctccaccaaa atgaagccat actggatgtt
    14581 gctggccttc ttgcttaact tctttgacat caaaacacgg cagtagaatc tgcatttcat
    14641 tgaattcttc gatagaattt tgccaatttt taatcgaaac cataattaac ctccttaaac
    14701 taatatgtta gttttgatac attttatatt ttaaaacccg ttgtaaccgt tgtcaatata
    14761 aaaatggaat aaacttgctt tttttctacc atatcagaat tattgtattt tttattcaga
    14821 aaaataaaat actccttctt tttggactga ttttatcgaa acttattttt ttcttttttt
    14881 catgtattct tttattttta taactagtgt attacttcaa aatttgatat tatatggcat
    14941 gaacagaatt aaaatatcag ctaaaataaa agttaaggag cattgagtat gacaattaaa
    15001 actagtctcc aaaatcaagc ttaccaatct attcgccgac aaattatcta tgctgactta
    15061 gaacctggaa aaaaagtatc agaaaaaaaa ttagaagaaa cactttctat tggtcgtaca
    15121 ccaatacgtg aagcactgat tcaactccga caacatggtc ttgttgatac aattcctcaa
    15181 agcggaaatt atatctcatt aattgattta gattcggcca aaaatgctcg atttgtcaga
    15241 gaacatttgg aacgacaaat tatgttagag tgttgtgcca aaattaataa ccaaaaaaaa
    15301 cggattttag aaactataat ttcagaacaa gaaaaagctg ttagtcaaaa aaatgctcgt
    15361 agtttttttc ataatgataa tttattccat aaagcttgtt ttgaaattgc agagcgagaa
    15421 gaagtatggc aatggttaga aaataacaat acacacttag aacgatttcg ttggctacga
    15481 gtaaaaacgg aaggattaaa gtgggaaaaa attatggaac aacattatca actatttgag
    15541 gcattaacaa aacaagatcc agaagaagct aactttctaa catcgttgca tcttcattta
    15601 atgcttaatg agcaaaatat tgttttagct actcatccaa aatatttcaa aagtatataa
    15661 cttttaactc tttttaaatt aaatgattgt tctgatcaga aaaatatcgc ttgttttctt
    15721 attatgtaat atatttcata aactattctg cataacaact attgtttgta ctttttacat
    15781 tggataacta attatgactg ctgataactt ttgatattaa actagtatct ccaatccaaa
    15841 taaaaaatag atatcttgtt catcaaaatt agttcatgga tacttacctt catttacatc
    15901 attcagcggt ttctcatttt cttttattct attatttcat tttctaatag acttggcttc
    15961 atttcgcaat acttttttac aataggcatc aaattggtgt tgtttagata tttagttgta
    16021 gttttgtgac atgataagta tgctcctttt tgtcttttcc actaatacaa cgatcaaagg
    16081 gtgctttttt caaaaaacta gaaaaatcaa aataagattc gtattttgat tctatatatc
    16141 ctgtttcaac tacatcttat atttcataat agaataaata gccttttgca tagctgctta
    16201 acttataaga aattgcaatt tacttcttca aagtgaaaac gaatatgagt aaaataaaat
    16261 tattcttctt acagataatt aaaaatagcg cgtaacaaaa atcggcattc aatcacataa
    16321 ttaggattga tgccgattac ttatattcta cgtatagttt tagtattctt tttttatcac
    16381 cagtaaccaa ttgagaacag tcctaaagca gaatagtctt ttatctcaaa acgcttaatt
    16441 tcaggcttag gaatatgaat aatcttttga gtatattctt tctcttttaa tcttactgaa
    16501 gaaataccac tgcctatctt ttttacaaat atttcatttg gtgtacagac taataaaaca
    16561 atatttgtaa tcaatccttg cactaaagaa ttaaaaggaa gactatttaa aacagggtgt
    16621 gcataactat aacatgtgaa gtagttattt tgaactttaa cttttaaaaa ctttaaaaat
    16681 aatcttattt gttcttctaa acaatcatct atactcatat aatcactcca ctaattactt
    16741 cccccaacaa gaaatagcat taatagctat accagctgca atagttgcag cattgaattt
    16801 tgcaaattgt gcaggagtta aacttctttt tgctacatct aaaagtattt taacagcata
    16861 atcataattt ttcccttgca acgcattaat taaagtttgc attagatttt cacgattaag
    16921 tatatcccaa acaacacacc aaatggtaat gagttcataa caacacatgc agcatacttt
    16981 tgaagatcac gtttttgacg ctttcctgtt ggctgcacaa aattttctaa aataaatttc
    17041 cctgctatac cttcagagcc atctaattca gcttttttct gtatagcttc taaatcagta
    17101 actatatatt gatgtgtttc tgtatcaaca taaccagctt cttctaaata gaatttcaaa
    17161 ctctctactg cctgttgtct ttgtgtttct atatcaggaa cttcattcac tttttcagca
    17221 gcataaacac tggttccggc accgactaac gtacttaatc caattaacat tacacctaat
    17281 aatgaaaaaa acttaaaaaa acgactcatt tttatccctc ctgaaaagta ttgttgatta
    17341 tcaattttta tccttttaag ctgttcaata agacgttttt acgttccttc cgtttctatt
    17401 ttattctttc tgtcttataa cagctttttt caagtaaaga acgatataat tcttcttaaa
    17461 aaacagtcta ttttttcggt atataataaa attgtattgt tacctacata gcgaaacagt
    17521 accaacatca ttcaaatgaa gatagcaaat ttgcttcttc ctttttctta agtgactatt
    17581 taaataacca gaaaaaaact aaaatcacac tgatatccca aataaattga aataaaggaa
    17641 aaagtttttt tctaccatga tagtttttca aaaaatagaa actcgtacta tttaaaagta
    17701 aaataactag catcgtaaag actactgatt tatctaaact tctataaaaa agtgggctaa
    17761 ataataaagt aattaaaaaa tgaactattt gatattgttt tttttcatat taacagatcg
    17821 cagctcctgc gattccatag gctgttccta cagcaaatgg aataacggct gcacctactc
    17881 ccgtaaggct taaacctaga gcagcagctg tataaatccc agaatgtaga agtccagccc
    17941 cagtgaaagc ccaactgcag gcacttcgtt caccagtttt attatttgtt ttttgatctt
    18001 tcatataatt ctcaacttca tgaagctgat cggttagcca tccatggcca ttttttttga
    18061 atagaacgat taaattcatt tagatactgt tggatttcag aaactgtaat ttcagaatta
    18121 attctatcta aacttgattt taaatcactc tcatcaataa tatatactaa tcgatttcct
    18181 ttagaatctt tatgtgtggc taaattattt tgaatgatat ttatttcagt ttcttgttgt
    18241 tttactaaaa aattatttgt ttcagaagca tcgacactta ttccagcgct aacaaaagta
    18301 cttaaaccta ttaagaaaac acctaaaata ccaaatattt taactattct tttcataatt
    18361 tttcctactt aaaaataagt tatttttaaa atatcttgat tatgaattca tcccccaaag
    18421 gccttttaag attgatacag cagtccgttc acttctttta gacttgtcat ataagtcttt
    18481 taaatgatcc cataccagac tagtttgtct atctagcttc aatccattaa acgctaatga
    18541 acggttcatt ttttctatta ctttagataa agctacagaa atttttatac cttcacaacg
    18601 cgcatcttgg atttcaataa tagtatcatc aattagtttt tgaatttctt tcctatcaac
    18661 atattcaatc ttagaaagag aatcttttaa agctactaat gattcaacga tttctttttt
    18721 ctcttcagac attctcttcc tccttctagt gacccgtaaa gccatgtaga gcagagtcca
    18781 ttgaaccagt aattacacta tctacaacgc cattccaatt tactctacat tttccatttt
    18841 tcagatttgt cggtccatac ggatagactt tttgacattc aatgttcgga ttataccaga
    18901 taggattata ccattcagcc gcagaagcag attcagtcga tacaaacaat cccacaccaa
    18961 caaaaccaat acacatcaca ctatagccaa ttaatttttt catctcgtat ccatctccta
    19021 aaaaatagaa tagttcttgt agttattcat aagacatatt ttttccgttt ttgttttatc
    19081 ctttctgtcc tataacaact tttttcaagt aaagaatgac atacttcttc ttaaaaaaac
    19141 agtctgtttt tttctatttt ttcggtgcat aataaatatg agtgttcatt ttttatttta
    19201 atacacactt cgtacataat aaatcttgat cgcatcaatc ttttgagctt cttgatctac
    19261 tttcaattga agtaatacaa attgagtgac ctcattcgtt gtttcttctc caaataacaa
    19321 atattgatcc ggattctcta tatcttgtgt cacctggtga agtttcccag ttgttttcat
    19381 ttgtacatgc gggtctacat caatactatt gtctttaata caacgatccg tcatatagtc
    19441 cctcacagct tgattgcgtt tataaacatc tgtgaaatta ctccattttc tagcaaaatc
    19501 ttttaagaaa ttttttgtca tttcatctat ttgttgcgct gtctcgtctt ctaatgaacc
    19561 aatttgactg ttactttcag tactttgaag agttccactt gtttctttta ctgcttcgat
    19621 tgtttcttct gtcgttgtca attgtgtttt ttgtttttca gaagaaaacc aatgacaacc
    19681 agtaagacct atcaaacata accaactaaa tactaaaatt attccttttt tcacgcttct
    19741 agacactgcc tttcttattt caccttacga accagactac gaatttttgt ttgattgtac
    19801 gtgcgttgat accgagccat aatccgtcct tgctcggcat tctgttccac actagagaaa
    19861 tgttgtccgc catctgaaac atctgttata attccggtgt gaccatagat tccaggagac
    19921 aatgcaccac ctgcttgcca attcaccaca tctcctgctt gtaaatcact tggttttggg
    19981 ttcatgatca cttgccaacc ataggctgtc caatcataat cttcccctat taacatcgca
    20041 aacatgtgtc cagaacccat taactgtggg ccacctagtt gggatacata ccacgccgtg
    20101 ccaccataac actctccatt atttaccgat cgtccaatga tccgatctaa aactttcgca
    20161 ttacctttac ttttccctgc aacaagtgac gatttttttg ttggtaggct attccaatgt
    20221 ttgatcgcaa aagtatcata ttctgttaag ttataagact ttatgatact catcgctcct
    20281 tggtaatacg gactgccttc ggtaatacct gccgccgcat agcctccacg acctagaatc
    20341 aataaacaac tcccaggatc tttattgccc acacattgat taaagttgct ttgactcgcc
    20401 accaaaaagc cgtaatagtc ataacaatct ttcaggttcg gaaacttcat atagaagcca
    20461 ccttcagaac caccaatccc ccgtgcagaa ccttgtggat agggacaacc aggaaaccag
    20521 gtaatcccaa agaagtttgt atcgtttctt ccagattgtg attgtccata agtcgattca
    20581 taacctaatt gagaaagaat cacacttggt aataactgat actttaccgc tgcaccgact
    20641 gctgcttgcg catgttcatc tgtgatcgga ccacgtcctt taatctctga aaaaatcggt
    20701 aatccttcgg tatattccca attaaaagcg acagctagac ctgtagcctc tcctccatcc
    20761 tcggaaagtt ctcctgaaaa aaagaatggc aatagaaaaa agggaagaat aaagaaaagc
    20821 agacaaccta gacacccttt cttcatttct tcctcatcct ttcttcgata atggtttttg
    20881 aactgttcgt ccttttggta aagtaaaatc ggttcctggt tttgcgtcca aaatcggcaa
    20941 tgtttcattt ttcttttcta tttttttcga ttgctctact ggattattaa aagaagaaaa
    21001 tgcaggttgt aaggattttg gtacggaaat cgacggttta ctttcagacg aacgttgtat
    21061 ctgtcgttgc acagaacgat ttcctgatag agtggctacc gacgacttag gaacagagta
    21121 ttgattcaat aactgctgca ttgctggata actcccattt ttcgcctgat ttttcacttg
    21181 gtgaatctgt tgtgaaagag aaccagcagc tgttaattgt ggcacgggta cttttccagc
    21241 aggcaacgga atcgattgac caacaaaagc gataccttta tttaacaatg gttttctttg
    21301 attattccct tgttgcttac tcgattgtgc caacgattgt acctgttgtt tcggtcttac
    21361 tgaattcatg gacgaatttg gaatcaaacc atttgaaaga ccagaattcc actctgtttt
    21421 tgatttggta gaagacattt caggctctac cccatgataa cgaacatgac gaccactata
    21481 aggcaagtct tgatttgaac gcccacctag ttcaaaggga ttaatcactt gccccccttt
    21541 taccgattct tgtggtgtac gtttttgttg aaacttttga aatccatgta tcgctgcaga
    21601 agcgagtgga ttcgttgctc ggtacacatt ccctgctaat cctgcacctt tgattgccaa
    21661 attttgccca gcagttgata gttgaccagg cagttgtttc atttgagaca aagaatgact
    21721 tgctatgttt ttcgtgttat ttatggtttg atctgcagct cctaacccta gtttctttaa
    21781 taaatctttc cgttttacaa tgagtaaaat tgataggagt gctttgacaa acatatttcc
    21841 taaaaaacca aaaacagtat tcattagaat taaatcgtct actatgttat agatcaaaaa
    21901 tgccaccaaa ataatcacac tgtaaaaaga tttttgaaac atcaagctca aaaaggactt
    21961 taaggtattc atgatcatat aatcaaatgc tggaacaaaa gaagcgagta atgaaaaagg
    22021 aatcacaagt aataaacaca acaccaacat ttgaaagata aaacgaatca tgccaatcac
    22081 tacaagtggt aatccaatac ttagattaag caatggcacc atagaaccaa cgactgcttt
    22141 ataaacagct ttagttggtt gaatatagac acgataagcc ttctttttcc ctctatcttc
    22201 tcctgcagca gtggtcactt ttgtctcaat atcagataat tcttcacttg acgcttcctt
    22261 ggctagaaat tcttgtggtt caatctctgc ttgatacaat tcttctaaat ttgttgtccc
    22321 ataatttaac aatagatacg gatcaacaac agccgcttta taataaagtt ggcgcacttt
    22381 ttgaatggct tccttttgtg tcgttggcac taaaacacca ttgacatctt ttgtcgccaa
    22441 ttcatctaga ctatttgtag cagaaaaaat aaagccttct acctccacgg acaaatcatt
    22501 taacccttta gtaaaagctt ctcctttact aggtacgtta ctaccatagc caaaccaaac
    22561 cattgaaaaa atgacaacaa agagaaatcg aataaacacc atttttgcgt aatgtttccc
    22621 tctaaatata cgaagagtaa atagatataa cacatacaag acgacaatcc ccgcaccaat
    22681 aggatcgtat attttcgaat aaatagtatc cgaaaaagtg aaaaactggt gaattaactc
    22741 gttcattgca gctccttcat agagcttctc aatgacaaaa tcaaggcctt gccaaatcaa
    22801 tttacttaaa ctaaagaaca tattcgtcat catttttaga aaatctgtgc catttgctcc
    22861 aaataacatt ttgcctaatg aaaattcact atcagataaa taagaagaaa aggtatcaat
    22921 atgaaatttc ccgatctcat agatcgattg atccacctgt ttttctaccg catataccaa
    22981 tccaccaaca aaaaacagtc cgaatccaac cgttaaaaaa agccaaacgc accatttctt
    23041 ttccttacgc tttatataag ccattgtttc acctcgtttc tattgcatga ttaacgtatc
    23101 ctgttcttct atttgagagt gacttgtttt ttccgttgtt tgtaatgcag cggtcatttc
    23161 ttcaaacaaa ctgtgtacat tgagttttcc tacacgtcca tagggatcta acatccaaca
    23221 ctgtccttta atcatatggc tcatttcaga taagttcgct tcatttactt ccaaacgtaa
    23281 atgagtcaaa atcttttctc gctcactgtc ttcatcaaaa gcgaagacaa caccaaaatt
    23341 ccctttttct ttatctgaac ctaagtcgcc aataaattgt gtatctagca ctaattgatt
    23401 catctgtgaa cgccccacac gcgccattgc tgcaataata ctacgtcccg cgttcgaatt
    23461 attaaatacc catgcttctg taaaataaat cgctgttttc ttactactat ctcgttttcc
    23521 aaacatttca caaaaacgac ctaaactaat catcaaacac aaagactttc gatccgcatc
    23581 tgtatatagt tctggtcgga cattgtcatt tggtaatttc aaatcttgga tctctaaaat
    23641 cgttgttttg gcattaaagt ctaatcccgg gttttctcca taagaaaatc ctaaacgtaa
    23701 aatcgaatct tctaccgtca agcgtaaaaa ctcaccatat tctcgaacct ctttttgttc
    23761 catttgttct aaatactcga ccacatgcat caagccaact tgttgtccgg cttctttttg
    23821 aactaaaact tcttggattc cctttaatac cgcccctttt aaaaggtgat tggtacctaa
    23881 cggacttaac tcatcaatca tatcctgggc tacttgtttc gcatctactc gggataaaca
    23941 aacaattgga tctaatacgc cataattacg gggatttgta gcatctaacg tgacataact
    24001 cattttttgc aaatgttcca caaacaaagg gtaatgtttc tgataatacg ggtcttgaat
    24061 gagtttcgta aaccatttgc gcttttctcc tttgggatcc acaaataaac tttgtacatt
    24121 cataaacgaa ctatacaaaa agagcaatcc aactaaaaag gtcttccctt taccggtttt
    24181 tccagttact gctacatgag gcgcatcaaa caacgcacct ttgattcctt ccgcaatagc
    24241 tagaagattc aaaaagacaa aacgatttga cgcataaatt aattcttcta atggtgtttt
    24301 ttctcctcga gaaccaccat cccctaatgt atcttgccga ccaatatacc agccggattc
    24361 catacctaat tctgatactg ctgaaaacaa ccattcgcca attgcttcta cagttgttcg
    24421 gtgcttccaa tgcaactgtc gacccaatgg ttgccctaat aaacatttat agaacaattc
    24481 tcgttgatca ttttttgcac ggtggatatg gatagtcgta tgaagctgtt gaataatcgt
    24541 tgcactattt tgacgaactt cttcaaccgt ttgaccgtat acaataaaac atgcgatcca
    24601 atcgacgaca ggttggccat tatctactcg ttgctttaaa tgttttacta atccccgaac
    24661 agttaataag cgttcgctag gttcttcatc gttttgaacc aattccttat attctgtttt
    24721 gaaccgttcc gatagccaat ttagttttaa accaatacct ccctgtcctt tcaagcgtgg
    24781 atagtgaatt ttcattcgta attctgctgg gaaagaaaga tcctgtgcaa attgaaatag
    24841 atcacagttt ctcatatttt caggtgtttc tccaataggt aacaacgcta aataactagt
    24901 ccccaactca tcttctaatt gaagaaggcc tttatgttcc ttcggcatac gtacatacgt
    24961 tccttgattg tctttcagta cttcccaatt attttgttct tctactttat gagccattcc
    25021 tcgaatatat tgcagacgat tgagatacaa cagctctttt tttactaagg gacgaataga
    25081 aaagctataa agtaattgtg tcacttcttt tacataagga tcactttcag aaaacaaagt
    25141 ttctaaatca tctacttcta atccaaataa gcgtgctaaa cttcctgcta catctaacca
    25201 gattagattt agtacatcat aaaaactctc cacggttgct ttttctttga ctttgattcc
    25261 taaaataaag gaaggacgat agacttgacc ttctcgttcc aacaatgtca ctgtttcttt
    25321 tgcataatac gcaccaattg cttgactatc gggagaaaat ccctttccta atgtgtcaaa
    25381 tttcgtccgt aaattcattg ttcgtggata catcaccaac tccacatctt cataatctgc
    25441 taaaagtaac agtaaacgag ccatcatttc ttttatttcc tgtgtctttt ttggtgtgtt
    25501 ttttgaaatg gttcgtggaa aaacatgata gtaggcccag atacaatcct cgccatttaa
    25561 caaacaattt ttctttaatg ctttcactgg ttcttttaca agtaccaata gatctcacct
    25621 cccaattaaa aaaaggaaca accagttgat tgtccctttt tctaatcctc taaataaaca
    25681 tacttattcg caaaaagtaa ttcttccaat gcattatttg ataccttgtc ccagttataa
    25741 tacacacaat caccattcat catacgcact acataacaaa accaatccca taaataaaaa
    25801 tgcacttttt ttccatcagg ttttagtttt gccagtgcta acacacataa atagggtagc
    25861 cctataaaaa aaacaacccc tgctggtgga aaaaaagaaa taacccaacc aataaatgaa
    25921 cgtaaaaata aatattcaaa gattacgacc aataaaaaag ttgctgcatt gactaatgca
    25981 actggaaatg gaagacgcca attgcttgta atctgttgta cgcgataagg ctccgcataa
    26041 acaatcgaat aatcgtaagg ttcccaatcc acttcactat cctcctaatc cataaataat
    26101 ttttcaatga ttttaccaaa cgcatcaaat acacgcgttg gatcatttaa gaagaaatag
    26161 actgcggcac ctgctgcgat agatattaat gcatttccta cttttgattt cataaaattt
    26221 cgcaatccca gataaacaac caccattatt aaaaaatagc caccttgcgt taatataaaa
    26281 tctacaattc ccttcagtgt catttccgtt cgtctccttt cctatccaac caattatgtg
    26341 tgagtttttc aacataatac tgatttgttt ttttcgtaat aacaagcgtc atttgttctt
    26401 tttgtaaaag tgacgtatca ccatctcgaa agacgactgt tgcttttaca ataaaagttt
    26461 ttttcgtttc tttataacgc aatactttca tctctacttg atcaacttta gctgtctgat
    26521 tgagtccttc aggttctttc atcatgtatg acatatcttt tgcattgttt ttcgcatact
    26581 tatctaagaa atcttccaca aatccttcca catctttcgc ttccgacgca tcaaccgttt
    26641 cgtataatgt gtcatcataa gtaatcattc gttgtttttc tgtattcttt ggtagtgccg
    26701 taaaataagg ataagcaata atacgacagc ccttgtttga aatagaaaag ggaagattga
    26761 gtacttgtgt gattgtcaca cttgtttctt tcttattctc tggatccatc gagcgaatat
    26821 cataagttac tttataacta gctgttggaa tggtttcaat ttcttttaat ccaataaacg
    26881 aagcagcagt taattttcga atacctgaaa cacttcctgg ttcttgaata aagttttgtg
    26941 cataaaactc tttttttaat atttcttgac gttgttgttc ttgttcatga ttatcgaatg
    27001 tattcatata caaagcaaca aaacgattca taaaggattc aatttcaggt gtgtacgtta
    27061 attctgactg actttgtact tctttctttt gtaattgttg gatttcatct ttcaaaaacg
    27121 acacttgaac cttcgttttc atggattgaa aaaatgccaa gccccctgaa aaacaaagaa
    27181 ataaaaccaa acaccaaatc catttcactg ctttttttga atggattttc ctttgattcg
    27241 gtactttctc tgtataaact ttttctttcc gccgtagttg tcgttttttc ataagctact
    27301 ccttctagta tgtttttact agtacgtacg attgacaacg ataagcttgt ttttctgaaa
    27361 aatagtccat tcaattaccc tcattttcta ttccttttct ctttctttta tatccaacaa
    27421 atccagcaat acttgtcaac actcctccta gaacagaaag tacaacatgt atttcttcac
    27481 cagtctttgg taagactttt gtttttccat ctgctgtttt gacttcaata ttgccacttg
    27541 gaagtttctt tacaggaatc gacaaatcct ttaccaaatt cccttcacta tcttggatta
    27601 atggaattcc atcttttaca ccaacaattg tttgtccacc ttctgctttt agtggagttg
    27661 ttggtgttgg aatatgggtc aattcctcta cattactagt gacaatcggt acactcattc
    27721 cttgagaatt attcgtcgta atttcaccgt ttggggttac cgatactgtg acttcttttg
    27781 ctggaacaat aggtttattt tcttttggaa ttgcttctga tttttcagca ggttgtttcg
    27841 gttcttcttt tggttcttca ctggtaaacg acatcgttgg ctgttccgta cttgacgaat
    27901 cggtattcgt ttcagaagaa ctggttgttc ctgattgttc tggagttgtg actatacttg
    27961 aatccgttgt tgttgaactg tgcacacctt cttcttcaat tggatcagtc ggcaaagttg
    28021 attcaggaat ctctggttct ggtgccactg gctctttttc aggtttctct ggctgcaaat
    28081 gttcaggttc tgctggtttt tccgttgttc ctccatttag ctctgtctca acatctgata
    28141 atccttcccc tggcaatgtt tctacaattt ctgacttagg tgtttctaca gtttcatctg
    28201 cataaaccaa tgaactccct aattgaaacg aaaataatga aattcctagt gttgttacga
    28261 ctgctaatgt tgtttttttc atgattattc ctccaatttt ttatgcaatt cgtgttttgt
    28321 tcatagacgc cgcactaata ttatctgaac gataaaagaa ctcccgtccg ttttcttcta
    28381 acccaacata tggattattt ttgaataatt gaatcaatag ttcactggtg ttgtattttg
    28441 gatagtttgc aaaaaaataa tactgtgctt gtcgcccatc cgaaaattca acaagtaatt
    28501 cataacgata ccgcttcaat ccttttcgtt gttttaaagg atctctatac caacttgtcc
    28561 atcgttccgt tcgtggaccg agatagacac gatacacata gaacaatgcc ataccacata
    28621 ccaccaaaaa taataatcgc cacataaaaa ttcccccttt tcttctacct ttcttgaatc
    28681 accgaacatt cataccctcg gtagagatcc gaaaaatcca tcattaattt ttcgtataaa
    28741 cgttgttgca aatagcgatt gtttcgaata cataattctt catagccatc aatcacttga
    28801 tcttctttct catagcatct tgtagctcta tgacgctcag cttgcatgac ataaccaaac
    28861 tctgcaccat atcgttctac cgttaataac tgttgactac gcaataatgc aatataacaa
    28921 tcacaaaaat gttcagccga catttgattt ttctcacgtt tgtgaaatag gtattcttta
    28981 gaaagcacat tattcgttaa acaatctgat aaatagcgat aatctggcgg tcggttattt
    29041 tcatctgtga atttttccca taataaactg atttttattc gttcagcggc tagatagtct
    29101 tgttcgcgtg gataaaattc atcaatcgta caaagctttt ttatcacccg aataaacaaa
    29161 tccatataat gcgaaacgaa ctcttccttt tcaattggct ctcctaaaaa ttccagtgac
    29221 ataccatctt ctccttcctt aaaaaataaa aaagcagaaa ccgatttggc ttctactttt
    29281 ctttcctttt atagctcttt tttacggaag aatccgcaaa tgacattttt tcaaaaaatt
    29341 aatcggcaca agttcacgaa aacttggagc aattttagat agaatcactt ccccatcttg
    29401 taaaatacaa tcataaaaat catcagttac ttgatactta cgcttcttac aataattgat
    29461 aaaatagtag cgatcatttt ctatttccat tcgttgttct actaagcgca tacgaacatc
    29521 atcatagaaa taagccacaa atcgcccttc aagttcacgt ttttcaatgg gatactcttt
    29581 tggtaacaat ctattttttc gtcgtttata cattgctgga taccttagaa gaagttcatc
    29641 caaacgtctg tcagtatatg gcaactgacg aatcaatgat tccaatcgtt ctttatacag
    29701 atagaataat tggcgcaagc aatagtgtac tgcttctgga ttttctaata ctgttggatc
    29761 acgcgaccaa tacgcaccaa cataaatcat aatttttttc atttagacaa tcacaccagc
    29821 aatactaatc accgatccac aataggtaga acaagtggca tagcggccga tttcctctaa
    29881 ttccagctca atctcttcta aagaaagata cgtctgttct tctgttggct ggcgattcat
    29941 ttcccaagta acagttgcca acgagatttc ttccacaaga ggtttttcgt tgctaagtgc
    30001 tgccacacag ttttcgtata tcttcatttt atcgcagcct ttttttaccg ctaaaatcgc
    30061 aggtaccggc aatgaaaatc gataaaagcg aatgaatttt agcgttcctt ttcttcggct
    30121 aaattccaac tcttcgagtt cttgttcgta ccccttacga acttctaata agcgtgcaat
    30181 ttcttgccag ttttcttttt cctgataacg tccagccttt accgcttgtt caatataagg
    30241 aactcgaaaa cgaaaataag acaaatcaac catcttttca tttaatgctt caattgcagc
    30301 ctttaaatcc aaaagacgca gccattctgt tgtcgtatat gcattcatgc aattttccct
    30361 cctgacgttc ccatccattc tagatagctt tgttgctcgt catttagttc agcattctca
    30421 attccttctt gtacaaagtc tcttcctaaa atacgatctg cttcttgtaa taactttaat
    30481 gtcggagcta cttgtttcaa aaaccagcgt tctgaacgat ctaatgagaa tttttctggc
    30541 tttacggtaa aatccaattc ttccatcgaa cgaaacaatt ttgcccatcg ttcatcaaca
    30601 attcgtgcat cactttccag tcgatcccaa acaataagtt ttgcattaat taaaccaacg
    30661 ccaatctctc ctaaacgttt aggggaatga tccaaaataa acgcttctac tgcttgtgtt
    30721 gctttttcat tagctaggcg taactcatat cgattataaa tgccatattt cgttaatgct
    30781 tctatttctg aaattttctc acgattcgca atttcaaaac gtttttcgta gaaattgaaa
    30841 aacaatggac tctttcgcga tccaaaatac aaggataacc cttcgcttcg gaaataacca
    30901 tcatctaaaa aacaaccgcc aatattctga aatttcttaa agtccatttc taataaacct
    30961 gctttttgtt tttctagtaa ttcaaagaga tcaaaatgtt cttctgtttc caatccccaa
    31021 agttcatcta acgcaatatc gaatcgacta cattgtactc gtttataacc agcttccaac
    31081 tttccttgat ttttccaaat ggaatatagc catgttcgcc aatctgttcg ttgttcacgc
    31141 atgacttcct catattctcg gcaaccttga cccgaaaact gtacaagtac attttgtgtt
    31201 acagtttgtt ttgaatgcca gaatacccag atatttccac gaacagaaag acaatcaaaa
    31261 agatacaagc ccgtcggacg tgtttcaaat tctgttagat ccattcgtaa cacacgttga
    31321 atcactattt tgtagtctgt cccaaaaaag gagagacgca aatagtcgat cattgctcga
    31381 agatctattc cgcttttcat atacaatcga aagcgttcct tgattcgttt ttcgaaatcc
    31441 tctgggattt tcttttttcc ttgttccatc ttctttatat aatctaccga gcatcccaat
    31501 aactgtgcaa aacgtttttg agagagtaat tttttccctc cacctgaagc gtgaatagca
    31561 ctcaattttt ttcgataatt tcgcaattca ataggtgtca tattctacct cctttataaa
    31621 ataacaaatt tcgattttct taatctgcta gaaatcccga tataacaggt ggtaccccct
    31681 aatttttcta aaaaggagta ctccttctaa ccttagctaa agccttgata taactgattt
    31741 aaatacactt ttttatttgt gttatgttat ttctgccccc catattatat atcggggggc
    31801 tttacacaaa gaggccgtgc gcaagcgcac gacctctttg tgggcacaca tgccaagcat
    31861 gtgctgccac gactggtctc ggtggcttcg ccgacacaag ccgcggcagc acatactcgg
    31921 acaatgtgtt ctcccatagt ccctatgaaa gccaccccga tatctaaacc aatgaatctt
    31981 ctttttcttg taattcttta tactgttcag gagcaaaaca ctgtaaaatc gctaaacgta
    32041 actgtatgtt tttctctttg cataaccgat atacctgacg aaaacgcaat atatcctctt
    32101 ttgtcaaaac aatctgattt ctcgtaacaa acaatgtctc gcgctcaatt cgtcggaaaa
    32161 cataagaacc ttttgtgtag tattctactt tgttaatcca ctcgtataat cttcgaaccg
    32221 caaaatcagg tgaaatttca cccgcagctg aagaaacacg atacaaaact tcacccatac
    32281 ttcaccactt ccttctaaat tataaaagtg tataactttc ctcatctacc ccatttgttt
    32341 tcgtatttcc ttttaacgat tgcgcttgac gatcaatttg ttcttcgtaa ctagtaaacg
    32401 cctcttcttt cagtacagaa aaatcttgca caatcattgt gtcaaatttt ttaaattcct
    32461 caataaagtg atagtctttg taaacaaatg gcgcaaagaa ttctacaggc acatcaaaac
    32521 caggaaaagc atacccacga ccaggttgat ctttgttata aaacggctta ttcttaaaat
    32581 cctctccaaa agtcattaaa tagccaatgg gagataaacg gccaacagaa atacggaacg
    32641 ttaaactatc tcgaacacca cttggaaaat attccgcatc tggtcgctgc atcgctaaaa
    32701 taacatacat gcctgcttga cgacctttta atactaatgg ctgaattaat ttatcaactt
    32761 tgtttctcgt ttccatatcc aacgaaccat agaaagctgc ccattcatca aaaaagataa
    32821 aatgcggcgg tatcccataa taagcataat ttttccctgg ttcatactta ggctctttca
    32881 tcgttttcat ataggtatat cgctgattca tttcttgtaa cgcatttcgt aagcaaataa
    32941 tcatttcttc attcgttttg taaaacacat gtcctttgaa tacatcaata tcacctaatg
    33001 ccgctaaatc tgccgctttc ccatcgcaaa tatctactgt ccctacatct aaacaagctt
    33061 tgatcaatga atacaataaa taggtttttc cgccaccaat cgcaccagaa attaatagat
    33121 ttggcgattg atcatatacc caatccaatg ttttcattaa atgaatacgt ccattttctg
    33181 ctatacattg atcaatagga atccgaccag aatcggaaag aaatacataa ttgattcggg
    33241 ctttttctcg ttcttcacga attaaatcac gcaaaaatac ttcggataat aagctaccca
    33301 tttttaaaaa tcgttcgtgg aaacggccac catctaaagg aaaagaaacc actaattctt
    33361 gtttcttttg ataaaaataa acttttggta atctcatttt ttccttcgtt tgtttctcac
    33421 caacttgctt ctttgtataa acaaaccgat tgtcaattaa cattcgagca agtaactgac
    33481 gccaataaac agaagcaaaa tatccttcct ttagtcgcga ccaacgcatc aaacctaaaa
    33541 ttaatccact aaccagcaac aaatacatca caatactgat tccaggaata agatacattt
    33601 gccaattcga atacgtattt aaacgaggat aaaatacttt ccaactaaac caacttaata
    33661 gaatcaaaaa tggtaacaga aagaaaccat aataacaaaa ccataaataa cgattgaaat
    33721 aacgaattcg tcgaccacga tacttaaaaa aatgaggttc caattgtcac attcctcctt
    33781 tctgttttta cttctcctgt gctaaaaatt tttgacaaaa agcaactaat actggaatat
    33841 cacatttaat aaaaaaccat aatcgaaaaa gatttaatcg atttttatat gggtgtgcac
    33901 acatattaca tattcgtact acacgaatcc aaaccacttc ctgatggtct tgtttccact
    33961 gatacggtaa tgaaaaacga acaagatcac gtaaaaacat acactgccca ataaccgcac
    34021 aaatatactc atttgtttgt ataaaaatcg ttggattgtc tccaaaatac tcaatcaatt
    34081 gttggatatt ttgacaacaa accataatct ctcgaagtgt ttgattggtt ttgtccagat
    34141 tcaaaatctc cccttccttc taccttaaat agaaagagta ggaagacttc ctcctactct
    34201 ttaaaaaact tattttttct ctttttcctt agaatcagca cttggttgtt tttgatctaa
    34261 acctttttct tgtgattgtt tcggattaga attatttgtt ggagcttcta ctttctctaa
    34321 tcctttcgca taaatcacta aatctgaata ttcacgattg ccatcttgtg ctggaatata
    34381 taccacatga tattctggat caattaattt cacaggatca cgtcgtttaa aaccagtcaa
    34441 atctacatct gctggaaatt taactcgtcg tgcaatcttt ggttcatctg gatttccaaa
    34501 aactaattgt gctcgctgtg atcgaatttc tcccgtataa tcgcctgttt cattatcaaa
    34561 tacacgatcg gactcttcta atgcgccaaa gcgtacggct cctatacaag tttcatctaa
    34621 tgcttctgta atatcataat tatttggaaa ttttaatccc atcttacaaa acctcctcaa
    34681 tttttacacc agttaaaaag acaccgacat cttgatagcc agttgttcga cgctctgttt
    34741 gtcccatatc atttgtttca cgactattca ctctttcttt acgaaaaaca aaataagctt
    34801 cttcaaatga aatacgtacc tctttttctg gtaatcgtaa tcttttatcc tcttcagctg
    34861 tgagataaac aaaaaaagca ggctgttctt ctcgcgaaca ttctgcttgt aattgaacat
    34921 atcgcccaga cacaccatta atttcctttg gtggattagg ttcccgtgaa cccaataaaa
    34981 atacctgacc taaaaactct ttcttcatac gcaatgcttt ttctgccata tcatttttcc
    35041 tcctcttaaa attcacaatc aattctaaac gactctatat tacacaatgg aaagtaatgt
    35101 ttaacaacaa atggctcatc actaggagac atatctacta ttaacatccc gtctcgttca
    35161 tgcacagaaa tatcagtaca ctcctcatac gtacattctt ttccactcaa aaaacaaata
    35221 tttatatttt tcttttccat attctttttt cctccattat tttcttttct gtaaaaacca
    35281 agtaataatt ccttttccat cttccaacac aaactgatga tatatttccc tagcaagaat
    35341 ccacacacat gctaaaataa agaaaataat aatactacta aagatgcact catccttgct
    35401 gtcaaacgga aaaagatata tcataattgc ctcccccata attaagaaac caattgatac
    35461 aaacaaaaac tctaccatca tacaaaagaa tatttcttcc aactctttta catcctttct
    35521 aactttttat aaaaaaggag attattttta taaacacttt tattcaacaa ctattttcaa
    35581 atgaattaca cattatcatc tcaagtatag tttcaatcgt cactattgtt tggtccattt
    35641 ctagctttta tttaaaacat cgctatacaa gaaaaaagaa aagcactgaa accctaacaa
    35701 aagaaatgtt tattccattt aaatcttcaa ttgaaaatta tctatttcaa aaaattacaa
    35761 gaaaaaatat tctagaacga aaacaaacaa ttcaacatct aatccaaaca gttgaaaata
    35821 aaaatataga cttttatttg ccttatgaac ttatttattt tgccaaagaa atacaaaaaa
    35881 taattaacac aaataaacat tcgaaaaaaa tcctcttcaa agtacaaaga atgtattttg
    35941 aattctcata ctgctaccta gcagaactaa ataaagtaag aaagcaatta ggagtagcta
    36001 aacgtgattt cttctataga aaaaaagaaa ctgtattatc acataattat attctatctc
    36061 ttttggatcc ttaaattctt atttattaca ctcagtattt cttggtgtat cctcatgatc
    36121 atttactact caacacaata atttatttgc ctttgataaa aaattctttc taaaaactaa
    36181 acaagcggct agttgcctaa ccgcttgttc gtcctcttta gtctctgact ttatacaaat
    36241 cataaccaat caatcctgcc gcaaggacaa agatcatacc aatcatgatc cataatacat
    36301 tgtcttcaga gcctgttttt ggcaacatag gtaccattgc agcttgtttc acttctttta
    36361 caggttgtgg ttcgtttggt tttactggtt gcggtggttt tggtgcctcc acaaattgaa
    36421 ccgtttgatc tttgtttgtt agatcgacgt tatgttttgc gacttcttca aacgtttctt
    36481 tttcatcttc tgttttggct tggagccatt ccgcaaaact caatcctttt cctgcaaggt
    36541 tgcgaccgtc aacgatgaat tcaaacaaga attccatcat ttctttatca gccataaaga
    36601 ctttttccgc atgtgttcct tcaaatgggg tgccgtctgg tgttaagtag ccttgaacaa
    36661 tcagtttgta aatttgaccg attgctaacc cattaacttg aacccattcg acaacctttt
    36721 gatttggtaa agctaagatc tcttttccac catttgcatt ggtagctttt gtcgtaatgg
    36781 ttggactgac gattttgatg gtttggtctt tgtcggtaat atctgcgtga gaagccaata
    36841 atacgccttc tctaaacaga tcttcaaaaa ctacgacttc tttgcttcgc aacgcatgtg
    36901 cgtctaggat aaagtctaga ttgactgtac catcagttgt ttctgccaca aacgttaagg
    36961 tacttgagac ttcttttcca tctactagca ctggtttttc cgttactttg tccattagaa
    37021 caccgttgac tgtgtatgtt tttccaacga tcaaatcgtg gtatttcaca gcgtctgtaa
    37081 tggtgacttt cgttaatgga ttaacctctt tcaagccagt gggtttatgt gtagccgttg
    37141 tacgtacact tgggttcgtt actcgtaccg tttgaccttt gtcgttgatg tctttatggt
    37201 ttgctagttc ttcgtcattt ctaaaaagac tttcaaacac gaccatttca cgaccattta
    37261 aacctgtcaa atcaaaggta aatggtacga tcaccgtttc attttctgct ttggcaataa
    37321 agccaacagc actttctacc ggttgaccat gaacaagtaa ggcttcacca gttgctttat
    37381 ccattaagat tcctttgacc aagtaagctt ttcctggaag gatctcatta atttcaacgg
    37441 tatcatttaa ggtgaacagc cccgttgggt ctgcgatcgt caacccgtct tcaaatacag
    37501 ctgtggtatg aaccgttgga ttgtttaccc gaacactttg attgtcatca ttgatatcca
    37561 catgtaccgc aacttcaatg ccatcacgga aaagtttttc aaagacaacg attgtttgac
    37621 ctttcagaac actagcatta accacaaatt caagatcttt tgtgccattg gctttgtctg
    37681 caataaaggt caagatactt gtgatttctt tgccttcttg taaaattggc gcattcgttg
    37741 ccttgttcat caatacacct gaaatagtgt attctttgcc gacaaacagg ttggtataag
    37801 aaacactatc tttaatgacg atagtttcta atggatcaac cactttttca ccatcggctt
    37861 tatttgacgc ttgtgtttct aattttggtg gaatcaaata gttacgtaca gaaaccaata
    37921 acgtttcccc atcatctggc gcattttgtc tgaatgtaat agtgaatgct tgtttgcctg
    37981 tatttggttg gaaactttct aatggtacat tttcggtaaa gtaatagtta ttggcaacca
    38041 gaccttttac taaaatctca ccattttgat cggtggtata tttaccaatt gcttgacctt
    38101 ttccgttata tagcgtaaac gtcacaccgg caagtggtgt tttcttcttg ttccattctt
    38161 catacttgaa caattttaca cttttccgtg cttgacggtt cgcaaaatgg atcactttga
    38221 tcggttctgt atcattggta atcgagaaca ctaatgggtt ttgattcaaa acgtaattta
    38281 cgggtgcttt gatttcttcc aaacgatagc gctcttttcc ataagtaagt gtaccgttag
    38341 ttaggaaaaa gcctttttca ttggttttaa agatatccgt tgtttctgta tcatttggaa
    38401 tctccattgt gacataacga ttggctaaag tatcaaaaat cttgaattct gtattggcaa
    38461 gtggaatcac ttttcctgtt tcttgatcca ctttcaccac tttaagacgt tgttcaatta
    38521 ctttatcttc taaaacataa tggaaagttt gcccattttc agaaattgtg acttcaagtg
    38581 gttcaaatag tttataccct tcttttcctt tggtttcaga aagaatgtag gtatcaaaga
    38641 caacagtatt tttaccaaaa gtagcgtaac catcattgtc tgtcacaacg gttttcacca
    38701 ctttgccagt tgtttttgac gtcaaagaga tttcaatgcc agctaatttt ggtttgttat
    38761 ctggattggt tgaatcgact aaatctttat tagcaatttt cactaaatca atggttcctt
    38821 tgatcacttg atcttttgcg gtcgcattga cagtttttcc tggttcaaca acaatttcaa
    38881 ttgtttcatc agaaagtgca tagcctttcg gcgctttgat ttcttttaaa acatatttgc
    38941 caggatcaag atctttaatt tctgcctgta ctttgccgtt catatttttc aaggtaattt
    39001 ctttgatctt tttcccatct gcttgataca agccatacac cgcattttct aaagaagctg
    39061 cgccttgtgc tttcacaccg gtttctgcat cttctttaaa gacacgaatg tttccctgtt
    39121 tcaaaatagt caaattgaca acagagtcac caggttcaaa aggattcaat ataccaattg
    39181 tttgggcacc tggacgacga tacgctaatg gcgtacccaa tacttcaatc tgattgaaat
    39241 gaatttttgc cttttctggt gcttcttttg tgactgtgat ccatgcttca ttcccatgaa
    39301 tagatacatt gatccctttt ggcttttgaa tgccttttac acgttgtaga aagtcattgc
    39361 cactttttac ttttaacgtt tcgccaattt ttatcgtatg tgtttgatta tgaaaagtag
    39421 ctcttacatc aaaattatta agcagacgtt gaatttctgc ttttcgacga tcataatcaa
    39481 cacggatatt tttgattggg atttttgtgc ccaaagcttc ccaaatcata aattgtgccg
    39541 caatgtattg ttcatcggat ttgtctttat ttgtgacata tcctaaataa gagatcaagc
    39601 ttgttttttt ccgtacattt gaatttttca aatagttttc caaagcagta gctgtataaa
    39661 cttgaccttc tgcggctgag acaccaggct caatacaaaa cgccacttgt ccattaatct
    39721 caaaacgaac catataattt aaagtgaagt ttggattgtt cggactagaa gtaacaccaa
    39781 tattttcgcc ccaatcaatt ttccgaccac ctaaaacagc tgtattggtt gcacggcgaa
    39841 cacgaaccga acgattggtt ggtactgtta ctaacagtgt aggtgtacct gtttttggaa
    39901 tgtcctgctc ttttttcttt tgatcttcaa tactagccgt agattctttg tttagtttcg
    39961 ttggttgatt ttgactagct gcggttgaag agattgtttt ttcaacgttt tcctcatttt
    40021 ttccttcttt gtcagcagta gccgctggtt gttccactgt ttcttctatt cgttgctttt
    40081 cttccgcttg tttctcagaa gactctggct tcacagaagg cgttgcttcg gcagcttttt
    40141 ctccttctgg agtggacgtt tctgaagcag gggttgtaat tgaggcagtt tcttcaactg
    40201 gttgtgaagt aacaggacta ttccctgggt gttgtgtttc cccaatcgtc tgatcgagag
    40261 tcgttttgga attaaccgtt ggcgcttctg tcacaggggc ctgttcaaca gtggcttcac
    40321 cagctgggac cccggcttct gctggttgga cttctgtcac ttcagaagcg acttgggcaa
    40381 tggcttccac cgtaggttgt gtagccgtcg tttcttctgc atgtgcttga gcagttccca
    40441 ccaagaaaca gaattgagat aaggccacca atgcgagtgc tttcaaccaa ttcattttcg
    40501 tttttgcata cacaagaatt cctccttttc ctgagaattt tatataataa aacaaaacac
    40561 cgttaccaaa agtgataacg acgtgaactg tctcttctat ttttatctgg cgctcttatc
    40621 tgaacacatt tcatcaatga ttttaccctc ccactataat tacagaacaa aaaaagaacc
    40681 tgcttcgtgg aaatcaactg tccatttccc aattggaata ccagctttat tggcaacttc
    40741 accatcacca aatatcatgc cataccaatt actaccttca tccttaattt ctttccaacc
    40801 atatgacata gcttcatcta acgaatcaaa aatcatgcca ctgttgccaa ttgttgcagg
    40861 atctggctta agatcttctg gaatagaaat atcaatcaca ccataatgat ccgattcccc
    40921 agtattattg tcattactag ctactggttt ggttgttcta ctgacttatt ttgatacaac
    40981 aagtacgctt attcttcttt tacttctccg ttttcaattt cccattcaaa ccactccgca
    41041 aagtcttgct taaaaccatc tactcgatac ctattttgta aataggcatc ttctgacgtc
    41101 gtaggatcta aataggtagg tttattctct ggacgagcat agacacctac ccatgtttcg
    41161 taatctcctt caaaataact atctaccaaa tagccttttt ttctaagaat ctcccgaact
    41221 tgatttttaa ttattgatac ttgttcaaaa ccatcactca ttctatcacc tcaaataact
    41281 ctagattctc ataaatattc ccgacaattt caatcctatc aatgatctct aacaaatcgc
    41341 tacttatgct tccccattcg tataagaatt tcccttccgc aaaataaatt ttccataaac
    41401 ttcttcatga tcgtcccaac caatatcact ctcgacaaat tccacgttgt tcttatcttt
    41461 tagctcagta aatgccataa gttcaacatg ttttccaacc tcgaaacaat cacaccaccc
    41521 atcaaccgtt acacgtgtgc cttcaatacc taagaaactt tgtcctcgtg tattaaacac
    41581 taaaactttc cgcatttttt gctcattaat taatcacgcg cgaaatctag gaatcacaac
    41641 ttgttcgctt cttctttata aaataaaaaa actccaataa tcggaaatta ctatcttttc
    41701 ctcattcctt ttttaaatct tcatacttct tcttatcttt gtcctaacct aataacacaa
    41761 attttgaaga aaaactcagt aaaacctgat tcttttttta tcttcacaac aaccaacttt
    41821 ttcttcagat ccttttacta gaacttgtat acaaaaattg ttgaatgctg atacaatttg
    41881 cggttacttg aaaaaacgat agatgtaaaa agataaatag aatcattttt ttattctata
    41941 tcagtctatg attcattgct tatcaatcac ccaacccatt cgatgccgtt tttttaagag
    42001 aaatttaaaa aagaccgtac gaatctcgca ttgtccgatt acttttctag aaaagatccc
    42061 caattctgta taaataaccc tatcttcttg ttggtttcat tttctcatat aagaattcag
    42121 tcaatctacc cactatgacc attactacgt ccaacacaac cgacatatta accacgtaag
    42181 tattccctca cagatctccg aatttccatg gtatggattc ccatcctgac gctgcagagg
    42241 acaacatatt tcatgtggct ctgtaccctt ttgatttgga aagactacaa atcaatactc
    42301 atctataact tatcaagcga actttcgcta ggcttctagt gattactaaa ttgttggata
    42361 acatacctag cccattttat ctagctggta ttcataacag ttatttcttg tattacttct
    42421 tccgcttgtg ttgccattga gcttgtcaaa aagttactta gaaaaaagct gccagtaaag
    42481 caactttcaa caaacccttt ttgtttctta tcatatcaag aaacctcctt ttataaattt
    42541 ttctataata aaaaaacgtc gataacttaa taagtatcga cgtaaactat ttttatatcc
    42601 cgtaaatcaa aaattctaaa gaaagatttc ctatataagc atattggata attcgattag
    42661 tattgggtat atctctacca ttctcatacc tatctatgga ccattttgaa attgaaggag
    42721 aaaaaagctt tccaaactcc tccctttcta gataataatc tttcctaatt ttcctcatcc
    42781 ttaatcccat actaaaagaa tttatcttgt taaaatcgct aactctttca tcttttggaa
    42841 gtatattctt ataatcttct ccgtacataa ggtaaggaac agtgacctta ccaagatagg
    42901 caatattcat tagtttttca atatctggaa tattaatacc tttttcccat ctacttataa
    42961 catttttgcc tgtatagatg gtaaagtttt taccaaattt ttcaagcgtc tcttcccggt
    43021 tctttcggac ttctcgtatt ctttttccca taacaatttt atcgtctttt gataaactaa
    43081 atatctcact atcacgatta ctctccttcg aaatttcttt ttcattactt aatagaaaaa
    43141 tatcgatttt tttttgcatt tcttttgacg gatttctctt tctatttaac acataactga
    43201 gtaatacttc actaattcct atattatcag ctaactcttt gttaagtaac ttattcatat
    43261 ctctgtatgc aattagttga ccgatgagaa tctcaatctt atcttcctca tcttttatta
    43321 gagtttctat attcaaaatc ttgcttagag tattatctac gtctttaggg atttttcttg
    43381 accctttttc atatttaact aaagtacttt gtgaaatacc tgctaaatca gctaactttt
    43441 tttgagatag ttttttctct tttcttagag atttaagaac ccttccactt gaattatcaa
    43501 ccatgaaaaa agcacctcct atgagatgct ttcatcatat actgacagct aaaaatcttc
    43561 aagaaaaaat attccaattt tgttaatttt agtatcgccg tgataaccaa atgacagttt
    43621 aaaatctttt atacagataa tcctcagtgt tgcatctact caaaaaggtt gatttaatag
    43681 gtttttcgat tgattataag ttatttcaca ctatcttgct catccatttt cagaacggcc
    43741 atgaaggctt cttgcggaac ttcgacggag ccgatttgtt tcatccgctt cttcccttct
    43801 ttttgtttct ctaagagttt acgtttacga gaaacatctc caccataaca tttagccaag
    43861 acgtttttac gcaaggcttt gatgtctgaa cgggcaacga ttttttgtcc aatagctgct
    43921 tggattggca cctcaaattg ttggcgtggg attagttttt tcagtttttc aacgattgct
    43981 tttccacgtt cataggcaaa gtctcggtga acgataaagc ttaatgcatc gactttttct
    44041 ccattcagca agatgtccat cttcactaac ttgctttttt gataaccaga catttcataa
    44101 tccaatgagg catagccttt cgtgcttgat ttcaatttgt cgaaaaagtc aaagacaatt
    44161 tcagaaagcg gaatattata gacgacattt actcggtaat catctaaata atccatcgta
    44221 atgaattctc cccgttttcg ttgagaaagt tccattactg caccgacaaa atcatttggg
    44281 accatgatct gtgctttgac gaatggttct tctacatcct gaatcgttac aggttcagga
    44341 aaatctgccg ggttatctac tgtagcagtc gtaccatccg ttttattaac atggtaaata
    44401 acggatggtg cagtcgtgat caattctaga ttgaattccc gttccaagcg ttcttggacg
    44461 acatccatat gaagaagtcc gaggaatcca cagcggaacc caaaacctaa tgcttgagag
    44521 gtttctggtt cgaattgtaa agcagcatca ttcaattgca gtttttctaa agcttcccgc
    44581 aaatcgttgt aacgagaagt atcaattgga taaagaccac aataaaccat cggattcatt
    44641 tttcggtaac ctggtaaagc ttcagcggca ggattgtctg ctaacgttac tgtatcccct
    44701 actcgtgtat cttggacagt tttgatactt gctgtgatat aaccgacatc accgaccatc
    44761 aaaaagtctc tagcaactgc ttttggcgag aagaccccga cctctgtaac atcaaatgtt
    44821 ttgccattac tcatcagttt gattttgtct ccgggtttga ccataccgtc cgtgatccgg
    44881 acatttagaa caacaccgcg gtaactatca taaatagagt caaagatcaa agcttttagc
    44941 ggtgcatcta agtctccact tggtgcagga acatattcca cgatctgctc gagaatatct
    45001 tcaatcccaa tccctgattt ggcactggct aatacagctt cactagcatc gattccgatt
    45061 acgtcttcga tttcttgacg gacacgttcg ggatctgctg cgggtaggtc gattttattg
    45121 atgactggca ggatctccaa atcattgtct aacgctaaat aaacattggc caatgtttgt
    45181 gcttcgattc cttgtgctgc atcgaccact aaaacggcac cctcacaggc agcaaggctt
    45241 cgagagactt cataggtgaa gtcgacgtgc cctggggtgt caatcaagtg aaagatatat
    45301 gtttctccat ttttcgctgt gtaattgagt tctacagcat ttaatttaat cgtgattcca
    45361 cgttcacgtt ccaaatccat cgagtctaac agctgatctt gcatttcgcg agacgtcaca
    45421 gtgtgcgtca tttctaaaat acgatcggcc aatgtagatt tcccatggtc gatatgagcg
    45481 atgatcgaaa aattacgaat ctgctcttgg cgttctttca ttttctttat atccatagcg
    45541 tctcctcttt tcatataatc agcactttcc attataccaa cgaaaagcag taatttaaag
    45601 agtttttaat tagtcagcta tagaagtttt ttttgtagaa ttttgctata ctgttatcaa
    45661 gctaataaat ggagatgaaa aaatggatca aaaagatttt agagaaaatc ttgaattaaa
    45721 accggtcact caagaacata tcgatcaatt caatgaattg ctttcctatg tgttccaagt
    45781 gactgaagca gacatcgagg aaagcgggtt tgagaataaa cgagctttta tccgttccaa
    45841 aaaaccgatt ttggaagtat cgaaagtgtt cggatggttc cacgaaaata aactgatctc
    45901 tcaaatcgct atctatcctt gtgaagtcaa tattcacggt gcacgttata aaatgggtgg
    45961 tgtgactgga gttggaactt atccagaata tgctaaccat ggattgatgc aagatctaat
    46021 tatcgttgca ttggataata tgcgcaagaa taagcaatgg atctcttatt tgtatcctta
    46081 cagtatcccc tactatcggc gtaaaggatg ggaaatcatg tctgataaac tttcctttaa
    46141 aatcagagat acacagctac ctaaacaaat cccagtaagt ggtatggttg agcgattacc
    46201 agttgatcat ccagacgtat tttccgttta tgacaaattt gcccaacaaa accatggtgc
    46261 tttattccgc agtgattttc attgggaaga gtattggcgt ttcgaaaatg aagatgagcg
    46321 cacagccgct gtctactacg atagcaacca tgaaccaaga ggtgttctgt tctactgggt
    46381 agctgaagaa atatttcacg taaaagaaat gttctatctt gatcaagaag ctcgaaatgg
    46441 cttatggaac tttatttccg cacatttttc catgatctac tgggtacatg gagatattta
    46501 taaaaatgag cctttagcat ttttgattga agatagtcag atcaaagagc agatcgaacc
    46561 atattttatg gctcggatcg ttgatgtaaa agaattcctt caacggtttc cgtttgttgg
    46621 aacagctgat gcttttcatt tcatcattga agatcctgtc gctccttgga ataatggtgt
    46681 cttcgcttta acatgggatg aacagggaca agtccgtgtc ttaaatgaac ctataggaaa
    46741 atccgttcga ctggatatcc aaacattgac ttgcttgatg atgaattatc gccgtgcttc
    46801 ttatttggca agaatcgaac ggttagaaac agatgaagaa acactgaagt cactagagcg
    46861 tatcattcca aacatggaag cttactttag cgattatttt tgataaaaag gatgatgttt
    46921 agatgaaaat ttattttgcc gcaccaatgt ttgctaaaag tgatttgtta tataaccaac
    46981 agttagttaa agaaatcaga gagctctctt ctgagctttc gatctatctt cctcaagaaa
    47041 atgaagcaat caatgacaaa actgcttatg cagacagtcg aatgattgct cttgctgaca
    47101 cagaaaaagt gttggctagt gatttaatga ttgctttact agatgggctg acgatcgatg
    47161 ccggggtagc atcggaaatc ggtgtggctt atgcgaaagg catcccagtg atcggcttat
    47221 atactgatac tcgacagcaa ggcggaaccc acccgaaaaa aatcgctgca ctacaagaaa
    47281 cagctgaaaa tcaatttcac tacctgaact tatatacaat aggtttgatc aaattgaacg
    47341 gaaaagtggt ttcttcagaa attgagttgc tttcagaagt aaaaagattt ttagatggag
    47401 ggactttcag tgattaaaga aatcaaaaag gtgcaaatag ctttacttgc ttttggtgta
    47461 ttcgttgtct gctataattt ttatgaattt atcacacaaa aatattcgac ttctcaagga
    47521 atcacattta tcgtggaatc tttattaggg attgcattga ttttcatgcc acaagtcatt
    47581 ttgaaagttt tcaaacttaa aataccagca gcaatcgttt tgttttactg gtttttcttg
    47641 ttcatctctg tctttttagg cacagggatg catttgatca gtatcatctc tttttgggac
    47701 aaaatcttac atgcggtgag tccaatggtc ctgacagctc ttggctatgg attgattggt
    47761 tacttgatga aagatgctga gatatcaaaa accagtcctt ggttgttcct cttattcggc
    47821 tttgcgtttg ctggactttg cggcgtgttt tgggagttct gggaattctt atgcgaccag
    47881 ttcttaggca tgaatcttca acgttttgct gcttctgatg gcacactgtt tgtcggacga
    47941 gcagcattga tggatacgat gggcgacttg ctgacaaata cgataggtgc tgccttaatg
    48001 ggattgtttg catggtctca aagtaaaaaa gatgagcgtt actttgaaag ttataaatta
    48061 gaaaaagtga aaaataccta ataaaagagg ttgtgacaga agcgggcagc ttcaagaaat
    48121 ttcggcttat ttttcgaaga aactacttct ggaacactgt ttatttgaaa aaaggaggtc
    48181 tgacactagt tgtgtcagac cccttttttc ttttatagtc tcaatggaag attagacgat
    48241 ttcttctagc tcttctgttt tgaaatgaag ttgtccatca agtaaagtaa tcgttacttt
    48301 tgatttaggc ataacatgtc ctgcaacgat ttctttagct agtggtgttt ctacttcctt
    48361 agtgataaat cgtttcaatg gtcttgcacc atatgctggt tcataagcat tttcagcaat
    48421 ccacgttttg gcttcatcac tgattgtcag caggatttct tgatgttcca gacgttgtgc
    48481 caattgcgcc accattttgt caacgattcc tttcacgttg tctaggctca atggtgtaaa
    48541 taaaatcgtg tcgtcgattc ggttcaagaa ttctggtttg aagtttccac gtaatagcgt
    48601 gttgacttgt tcagccactg cttctggaat cgttccatca gcagtcacgc cttccagcag
    48661 taactgtgaa ccgatattgc tggtcatgat cagaaccgta tttttgaaat ctactactcg
    48721 cccttttgaa tcagtcaagc gtccgtcatc taggacttgt aacaagatat tgaagacatc
    48781 gggatgcgct ttttcgattt catccaacaa gacgattgta taaggatttc gtctgacagc
    48841 ttccgttaat tgaccacctt cttcgtaacc tacatagcct ggaggagctc cgactagacg
    48901 agacacagca tgtttttcca tatattcact catatcgatc cgtaccatat gatcttctga
    48961 gtcaaacaag ttctcagcta acgctttcgc taattcggtt tttccgacac cagttggtcc
    49021 taggaaaagg aaagaaccta gaggacgatt tgggtcttgc aatccagccc gtgaacgaat
    49081 taccgcgtca ctgaccgcat caactgcttc atcttgacca atcactcgtt tatgaagtgt
    49141 ttcattcaat ttaattagtt tttctcgttc accttctaca agtttcgtta cggggatacc
    49201 cgttaatctg ccaacgactt gagcaatctc attttcggta acggactctt gaaccatctt
    49261 aatctcgctg tcttttgctt tagcttctaa ttccttcaat tctttttcta attgaggtat
    49321 cgtaccatga cgcaatacag cagcgcgttc taaatcatag ttattttctg catcttctaa
    49381 ttcgtgtttt gctttatcga tttcagcacg ttttgctgaa acagaattga cttcttcttt
    49441 ttctgtttcc cactgcatct tcatcgcatt tgcttcttca cgaagttcag ctaattcttc
    49501 ttgcaaattt ttcaagcgtt ttttacttgc atcatctgat tcttttttca gtgcagcttc
    49561 ttcgatttcc aactgcatca aacgtctagt aacttgatct aattccgttg gcatagagtt
    49621 catttctacc cgaatcgttg cactggcttc atcgatcaaa tcgattgctt tgtccggtaa
    49681 gaaccgatca gtaatataac ggtcagataa agtagctgct gctactaatg cgttgtcatg
    49741 gatgttaaca ccatggtgga tctcaaaacg ttcttttaat ccacgcagaa tactgatcgt
    49801 atcttccact gttggttctt tgactaatac cttttgaaag cgacgttcca acgctttgtc
    49861 tttttccata tattgacggt actcatctaa agttgtggca ccgatcaaat gtaattcacc
    49921 gcgagcaagc attggtttca acagatttcc ggcatccata cttccttctg tttttcctgc
    49981 ccctacgata ttgtggattt catcgatgaa caagaggata cgtccatcac tttttttgac
    50041 ttctttcaag actgctttta gtctttcttc aaattcacca cggaattttg ctccagcgat
    50101 caatgcaccc atatctaatg aaaaaatcgt tttatctttt aaattttcag gcacatcttt
    50161 gcgtacgata cgttgagcaa gcccttcaac gattgccgtt ttccctacac cgggttctcc
    50221 tatcagaaca ggattatttt ttgtttttcg tgaaagaata cggatgacat cacggatttc
    50281 ttcatcacga ccgatgattg gatccatttt tccgcttttg acttgctgta ctaaatcaac
    50341 gccgtatttt tctaatgctt tgtattgttc ttcttggttt tgtgaggtca ctctttctcc
    50401 tcctctcatc tcttcgatgt ttttacgcaa ttctttttca gataatccgt ttttattcaa
    50461 ataaacagtt aacggataat tttttaattt catcaacgct aaaataacga cttccgttga
    50521 aagaaactcg tcgccgaagc tttcacgtat ttggtctgct tcattcaata atcgaaagaa
    50581 attctggctg aggttttgtc catattggac gtttccacca gaaacaactg gatactcatc
    50641 cagtagacga tcaatttcat gttcaaaaga ttcgacatcc aatccggcat ccgtatagaa
    50701 atttcgacca aaatgattag gttgtaaaaa gattttccat acatgggcaa tgtcgatatc
    50761 ttgatgtttt cgagtgaccg caatctgttg agcttcagca atcgcttctt gaagcgtcgt
    50821 agtcattttc tcaatattca tacaggtata ccctcctaat taaaggtaag atcacttatt
    50881 tctttgatct ttacaaaaac tattatatcg ctttggtcaa ttttggtcaa aatatacaca
    50941 tggttttttg actttttttc tcgacttata taaatcttaa aaaaacaaaa ttgattgtat
    51001 gtatataaaa aaagagccaa acaaacgtaa aattatctta tccataacgt ttttctttga
    51061 tttgataaaa agggtagatc ttataaaaga cctacccttg attttcttta ttctcttttt
    51121 ttacttctat tatttcttct ttgatcactc ttcgccgttc agcaatcaca tacacaaatc
    51181 catagagaaa tggcaagata aaggaactga aacgatacag taaaagcgaa gaaactgcgt
    51241 caactgttcc aacaactggt ctgaataata acaaatagac aaattcaaag gaaccaatac
    51301 cagcaggcgt aggaataacc cctgaaagca ctactgcaaa gctgataaac gaaatggtca
    51361 gtagaaaatc tattttagga tggttttcaa tcagaatcaa ataaggaagt acataccaaa
    51421 atacaagttt taacatattc catagataaa tccgcaatag cgcagtcctg tcttggataa
    51481 tggtttggac cgtttccctt aaagagtaga tctgctgatt gcatgtatcc acccatttcc
    51541 gcaaacgctc acttttaaat aacttattcg ataagctgac aaaaaatatt tgaacattca
    51601 agctgacgga catcgccaat aaaaaggcaa caatcacgat cgtcaaaagg ataccagcga
    51661 tgataaacca gatcattgat ggaccattag aataaaaaag agagaattgg atgaccaagc
    51721 cagctaatgc ataagtgatc actgccagtt tgtacataat catatggagt gctgtcaccc
    51781 ctgctccctg ggatagcttc attccttttt tgttataaaa ataaatctcc gagatcagcg
    51841 ttcctgtacc aaacgaaacg attcggtaaa acgcaatata gctagaggtc aagaacccat
    51901 ctttcgttgt aaaattcggc gcgaaaggtt cagcaatttc tttgattgag cgcccttcag
    51961 cgatttggta aactgtccct aatataacag ccgctgccaa aacaacaaca ctggtagaca
    52021 gcaattcggc aaagatatct gcaaatgatt gattgattaa ataataaata atggcaaaaa
    52081 tcacaatcag caaaagcaga ttcagaatga ttttagtttt cgttccttta gacgacatct
    52141 cctgctcctt aatggaaaaa ataaatgaga atcaaatcaa caagtacacc tgcagcgaat
    52201 cctatagtaa acttcttttt ccgagtttta tgatggaaaa cttttcgaga gatcaatcct
    52261 cccaaaccac cgcctaacag gctgacaaat atcaaattat tctcaggaac acgccagccg
    52321 ctagttctag cctgatgttt atcatacccc atcagacaaa ataagtaaaa attcacaatg
    52381 aaaaggtaaa accacaaaag attgtgtaac aatacatcca ttactcttct tccaactttc
    52441 tgttaactac tatttctaag ctttttcttc tttctttggt gaataaactg ttaatccttt
    52501 tgatacaaca gataattcaa ctggaagatc atctgtcgtg tctccatccg ttcgagtgcc
    52561 cacagatttt tcctgcgctt ggattttcat gcgtttggct gtgaaataag taatgccggg
    52621 gatcgtatag aatttgcctc tcatgattct aggcaaataa aaaataaatc ggaaaatatc
    52681 taaggaaggt acgatcgtta catgaaattt tccatcatct agggtcgctg agtcgtcaaa
    52741 atttgtaaaa ccgcccacgg agttagacat cgtgactgtc aacagttggg attttccttt
    52801 ccaatgctcc tcgtctgttt ctaaatccaa atgatatttt ttcttcttca gcaataactt
    52861 aaaaaacttc ttgataaaga tccacgagcc gtaacgctgt ttttcttctt gactgatcca
    52921 aactgctgta tctgctaaga ttccgattgt cagtgtagag ataatgactt catcattgac
    52981 ttgaccatag tctattttcc gtacattgcc ttccaggatc acgtcagcag cttcttcttt
    53041 tttgagggga atctcaagaa cacgtgcaag gttgttgaca gttccacctg gaagtaatcc
    53101 gacatggtat tgagataatg ttttttggaa cgcttggacg atatgatgaa tcgtgccgtc
    53161 tccacctata attactaaag tatcagcttc tatggcttct gctttcttga ctaattccgc
    53221 atcatcaatc gatgggccag tttcagctag ttctacatat aattctggct gcttttcttt
    53281 tacataatct tgaaaccagc gtgctaattt ctcaccctca tctttccctg aacttttgtt
    53341 ataaaaaact agcagctttt ccattcttta cctcctattt actcttcaaa aaagagggcg
    53401 ggacaaccaa ttccattgtc ctgcccttta gccttagaca gatgacattc catcattggg
    53461 acgaacgcct tgtcataagc tgttcttttt cgattttaac tctttgtgtt caattctgca
    53521 atttttacaa tcacgtctgt tgctttttcc attacttgta aagaaacaaa ttcgaatcgt
    53581 ccgtgcatat tttctcctcc ggcaaataga tttggcgtcg gaatacccat gtaagagatt
    53641 tttgaaccat ctgttcctcc acgaactggt tcgatcactg gagagatacc aagctcgatc
    53701 attgcttgtt tcgctaattc cacgatgctc atatcttttt caatgatttc tttcatgttg
    53761 taatattgat catacatatc tactttgatt cgttcttgat caaatcgctg attcaactct
    53821 tcttggatat gtgtgatttg tgctttgcgt gcttcgaatt tttcacgatc atgatcgcga
    53881 atgatataac tcatagaagc ttcttcagga ttcccatcca atgccatcaa atggaagaaa
    53941 ccttcgtatc cttccgtttt ttctggaact tcatcggctg gaagctgatt atgaaaatcg
    54001 attgctaact gtagagcatt gatcattgta tcttttgccg tccctggatg tacatttttc
    54061 ccttggatcg taatatttgc ttgagcagca ctaaacgttt catactgcaa ctcacctaaa
    54121 ggaccaccat cgactgtata agcaaaatct acatcgaatt gctcaacatc gaatttatcc
    54181 gctccgacac cgatttcttc atcaggacca aaagccactt tgatggtacc gtgagggata
    54241 gaaggattat tgatcaagat ctccattgca gtcatgattt ccgcaatccc tgatttgtca
    54301 tctgctccca gtaatgtact gccatctgtt gtgatcaatg tgtggccttc ataatttttc
    54361 aaatttggaa aatcttttgt attcaatgta tagcgttctt ccgcatctag tacgatcgta
    54421 gactcgccat catagttttc aatgatttgt ggattgactc ctactgcatt aaaatctgct
    54481 gtatccatgt gggaaataaa tccaatggaa cgcacttcat gatcaacatt gcttggtaat
    54541 gttcccataa cgaatccgtt atcttcatta tagaaaacat cagctaagcc taactcttct
    54601 aattcttttt tcaattcttg cgcaaaagca acttgtgtct gagtcgatgg agtcgttttg
    54661 cttgttgcat cggagcgtgt ttctgttttg acatatcgta aaaaacgtgg caaaagattt
    54721 tcgtacatag tattctctcc ttttaattaa attgaaatgg gtttgtattt gtttcagaaa
    54781 cgaaaaagtt aatatcccaa ttttcttctt gtttccaaga ttcaaatttc tcgataaatc
    54841 gttgcttgca taacgattca atataatgac cgggatcaat tgcaatcaac ccagcgcttt
    54901 gcatatcatg agccgtatga taatagatat ctcccgtgat atagacatct gctctttggg
    54961 cgattgcttg cggataaaat ttttcaccgc ttccaccgca aatcgcaatg cgcttcacag
    55021 aggattttgc attttttggc tggactattc tcagtccatc tagttggaac gcttctttta
    55081 cttgttctac aaatgcctct atagaaattt cttgcggcaa ttcacctact cgtcctaacc
    55141 caaacatttc gactggttca tctatagcga aaagatcata agcaggttcc tcataaggat
    55201 gagctgaccg catggcttga atcacttgtt tttcgatagt ttccggcaaa ataacctcaa
    55261 cttttgcctc ttgaacttgt tctgtcttcc ctactttccc aatcgctggt tgtgcaccag
    55321 cttccggagt aaatcgaccg tgtccaatcg aggtaaaact tgtaccggta taatcacctt
    55381 gtgtaccagc accagctgca gctaatactt cacgcatttt ctgtgcatga tccacaggta
    55441 cataaacggc cagtttttta taatgaatct catgagtctt tactaaataa ctctccactt
    55501 caataccgag aagttcacaa aaccagtcat tcagtccatc ccaaataata tccatgttcg
    55561 tgtgtgctgc atagacggca atatcatgtt tcaacaaatc tgcatacatt ttttcttgtg
    55621 gctgatcagt aaccagccgt tttacaggac gaaagattgg aggatgttta gcaatcagca
    55681 aatcgatttt tttcttgatt gcttcttcga ccacttctgg acgaacgtct aatgtcatca
    55741 ttactcgttg gattggctta tccaatgttc cgatatgcag tccgactggg tctccttctt
    55801 cagctagcca ctgaggacaa tagctttcaa acttttggat gaaagtgcgt ccgctgattg
    55861 tcatggcttt aaaacctcct caatcatttg gatttcctgc tcgatttgtt tttgacgtgc
    55921 ctcttgatta gctgcttgtt tgagctgttc acggatagct tttctttgtt gaagctctct
    55981 tgtccatttt gatttgaaga tctcactttt ctcttcaagc agaaatggcc cgaacatcag
    56041 ctccatttca ttgtaagtaa ctggtttttg ttctttttcg gctacaatga tttcatatat
    56101 ttttttattt tcttcaagta ttttttctga ttggatttta tactgatttt cttgcagcca
    56161 agatcgtaat gtacgctctc cgatgtttgg ctgaaggatc aaacgttctt gtccgcttaa
    56221 gcgttgattc aacttgccag cttctaggat atctcggatc agtgaacctc ccatacccgc
    56281 aatcgtgaca gctgtaatac catcttgtgg ctggatagca tctagaccat ttgcaaggcg
    56341 tacggtgata cggtcttcta atccatcttt tttgacttgc ttacgtgctg attcataagg
    56401 accttgaacg acttcaccag caactgcata ttcgattttt cctcttaata tcaatgcaac
    56461 gggtaagtaa gcgtgatctg atccaatgtc tgctaaacga gcggattcag ggacaaagtc
    56521 acccactgcg gctaagcgct gagataaatc taaatggttc atagcttcac tccttctaga
    56581 taattatagc attttccaaa taaaataagg agactaggaa taaaaaaatt cccatatctc
    56641 cttaaaaaat ataccttttc gttctataaa acaacatttt cttatttttg aaaaaatgtt
    56701 attggttatt tgttcaatga aacatcaata atcgaatctc caggaacttg cggtgggata
    56761 gacatcgtca tgatcggcat ctcaactaat gtaaattgga ctttgtcacc gattttcaat
    56821 tcatttatgt ccacgcttat tttttcagca gtagcattta agatcactcc gtcattgctg
    56881 aatgacgttc ccatattcgc tggatcttct ttggccatga catcgacgag tttcaactga
    56941 agcgtttctt cttctgcttc tacttgagta atactttcaa tcgttccgac aaatatgctt
    57001 tgtctctccg ttttcgtact gcttgttttt actgtttgac ccgcatcaat cgaatcaaca
    57061 gaacctgtgt tgctatctgc gctaccgcag cctgctaaaa gaaaaaatag accaactaca
    57121 gatatactag aaaaaatagc cctcataaaa aatcctccgt ttaagtttct gcattacttg
    57181 ttgcggtgat tgtctccggt catttctgtc catataatga ttcgctgacc tgcttttact
    57241 actaggtcaa atgtcagttg gacttgtcaa ctgttcaacc attgcgtcgg ttgcgaccag
    57301 tcatttcgat ttcttttgtc aaataacgta tgcgttccat tatccgtttc gcttttagcg
    57361 gctcttcatc tccacgttga gtgacggaca aatgctgttc caattcatcc attgtaaaat
    57421 tcgatgagaa gaaggttggt aactgttcct gcatccgata ttgaaggata acaccgaata
    57481 cttcgtctcg tatccaacta ctcatcgcat ctgcaccaat gtcatcgatc atcaaaatag
    57541 gtgaacgttt cactgcatct aacttttctg ccacttgatc ttttccgatt gcctgtttca
    57601 tctcaacggc aaatgtcgga aaatggacta gcgtcgtggt gaatccagca atcgctaatt
    57661 ctctcgctgt tgctcctaat aaatatgttt ttccaatacc aaaacttcct actagataca
    57721 gtcctttatg ataccctttt ggatcagcag tatatttctc tacaaaatct agggcttcta
    57781 agctcgcata tcctcgtcca tctgtacgtt caaagttttc aaaagaagcc gattgaatat
    57841 cttttggcat atccattgct tttactcggc ttctgatttc ttcttgtctt tgccgtgcta
    57901 acaatgcttc cgttggtaca taagtgacat ccacataatg aaaattcaac gtcagcttcg
    57961 gctcatagcc gggagcgatc atcgttggat cgtttttttg atatttacgc ttttcttgca
    58021 cgaattcata caatttagcg taactttttt caatatccgc ttgtgtcaat tcttcttggt
    58081 gtgcgcttag aaaagcttga acatccgcat catttaagac ttcagcgatc atttcagcat
    58141 acttttcttg ataattgcgt ttgctgatga tcctcttcaa ttctttgcct acgtcttcca
    58201 ctagatatca ccctcctttt ttgccaggta ttctttaaaa cggcggtcaa tctctgcttt
    58261 tttctcgctg ctgatctgtt tttcatcttt tggctggttg acccaatcag gaagcttttc
    58321 ccgtcgaaca tttcgcttgc cagtttgata atttgtttgc ttcttttgtt ttgattgttt
    58381 tgcttgatta cttatttgcc gaatgtgctg gatcgcttct tctgctgtgg cgattttctt
    58441 ttgtgcccat tcatttgcca ctgtatttac ataactggca tttaagctag cattattctt
    58501 gatcactagt acataattca gcaaaacatt gatcacgcta ctggaaagac ctgattgaga
    58561 aacaagatct tgcagtaacc agttttcttg tttagaaaca tatccgcctt tttctgactt
    58621 gattgcttcc agatatttca tcggtggaat gctctgtgct tcttttatca gctgaagcgc
    58681 tgcgtttgtc aaatgttcgt ttgacgaggg ttcattactg ttttcagaaa ccgcaactgt
    58741 ttcttttgtt tgcttcaccg gacgtctctg cattgccaaa atggcttttt gcagttcttt
    58801 ttcagatact ttttgttcac taaagtcata agattgactg atcagttcgc caagtgtcaa
    58861 ttcatcgaaa ccgtaaagct gatgatacat gtcgacttgt ttgatgattg gatcatcaag
    58921 acttgcaata tgtttttttg ccagccaatc attcaaaaac tgccaatcga atgtcgaatc
    58981 tgcttcaagt aatgctactg attcagattc ttcaaaagat ttattcagcg attcgatgtt
    59041 ttcttgatta gcagcgtaca ctgcttcatt gaagcgataa acatctaaaa atttttttgt
    59101 gattttttga tatccctctg ctgtatgcct tttaggttca aagaattggg taagcttctg
    59161 gaatcgtcgt tcaccaactt tttccagaag aagaaaagcc agaacagagt ctttgaaaaa
    59221 tgctttagga atgagtggtt ctaacagctc ataaataaaa cgaggtccca gatcactatc
    59281 ttctttaaaa taaacatcca gtaagccgat tccttctagc ttttttcttg catcataaaa
    59341 ttgcgggata ccgcagccta gactactaag cagatctgca tgcatccctt caggaccttt
    59401 gccagattct tgagagattt ctgataacag ggtgaaatag atactcaagg cttctccgcc
    59461 gattagtggc tgataaagat acagcaaggc gttcttgctg tcttctctca gcggagccgc
    59521 ttttcttcct tggtaaatat tttttggttg aagctctttc caagcatttt ccaaggaaat
    59581 gcctcctttt tgtttcttaa ttgttttctt tattattctc tttattattt tcttttgctt
    59641 tatccacgat atcttgcaac tcttttaaga aaacagacat atctttgaac tggcgataaa
    59701 cacttgcaaa acgaatataa gcgatttcgt ctaaatcaac taaatcttcc atcacatatt
    59761 caccaattag attcgtcgat acttcgtttt ctccgagact gcgtacgcgg ttttcgacat
    59821 ggttgacgat ttgttccatt tgttccatag ctacaggtct tttttctgct gaacggatga
    59881 gtcctcttaa aattttatcg cgattgaact cttcgcgttc cccgtttttc ttgattacta
    59941 ataacggagc agcttcaatt cgttcaaatg tagtgaatcg gaacgaacaa ttctcacatt
    60001 cgcgacggcg acggatcgca cgtccatcat ctgcttgacg gctgtcgatt actcgtgaat
    60061 tattatgttg acatctggga catctcattt tgtctcacct cttgcatcgc attttatata
    60121 aaggatgtgt ctttctttac aactagttgt attataacac gaaacaaatc acaaacgcga
    60181 ctgtcctacc caaaaaacac ggatataaga acaatttttt ctactttcat gcgtaattgt
    60241 gtcccctgtc cttactaaat ttcaattttc caatactgat caccttgatt actgagaaat
    60301 aacttacaca aaagctgatt tagtcatcta gcacaaaaaa cttgaagcag atgaacacct
    60361 tctcatctgc ttcaagtcac atctcgattt tttactattt tataacttct cttcgattcc
    60421 tgtatagtgt ttcattcgct gttcctcgat tttttgctca tcggaaatgt ggtcactttc
    60481 tgggagcggt gttttactac gatcaggtgt cggatttttt cgttcttgag tcaaatcttg
    60541 ccgcttcgct tcttcttctg cctgctggtc ttctatttca tcgattcttg cttggtactg
    60601 cttttctttt tcttctgctg tttctgattg cgtacgacct tcatcgatta gattttcttt
    60661 attgagcatc tcgccgattt tttcttttgc gttacctacg actttatcct tttgattttt
    60721 taatgatccc atcgctatcc ctcactttct atatactcag gatagcaaaa ttgacacaga
    60781 tactcaaaga aagcgattag caaaaagcca attttcgatt tgtcccacaa gtttttcttt
    60841 tgttccattg ttgtcgaaca aaatatccgc acgctgcttt ttttcttcga ttggaagttg
    60901 actactaatt ctctgctgcg cttgttctag cgtcagttga ttacgtttca tcaaacgctg
    60961 aagctgagtt tctggcgtag tataaacgac agctactgca tccatatagt ggtcataatg
    61021 accttcgtag agcaatggga tatctacaat tactaaagga tgattggaac ttagagaggc
    61081 gatttgatct tttatcgctt ttcggataaa cggatctaat agctgatcaa gtttttgccg
    61141 tttttccttt gatggaaaaa caatcatccc aagtttccct cgatctagtt gcccatcagg
    61201 ctgcaatacc tcttggccaa acactgatac aattttcctc agagcaagcg ttccaggctc
    61261 aacagtttct ctggcaatca catcgccatc gatcaaagga aatccatgag actgaaagac
    61321 tttggctgcc gtacttttac cggtagctat tccacccgtc aaacctaaaa caaatcctac
    61381 tttgcgattt ttagcctttg acaattcgga caatagtgcg tccccctttg tgccactttt
    61441 gttttcacga tcggcgttcc acaacgtata catggtttgc cagtttgctg atagacatgc
    61501 agtgctactt gaaatccgcc tgattctcct aatgcattca aataactgcg aatcgtggta
    61561 cctccggctt ctattgcacg atccaaaaca tcaatgatcg atagacgtaa ttgttctacc
    61621 tctttggggc gcagtgtgtt cgccggttgt tctggatgga tcttcgcttc ccataatgct
    61681 tcatctacat aaatattacc tagaccagtc actagacgtt ggtctagcag caatggcttg
    61741 attgctttgt gggacttttt cagtccattt gcgaaatctg ctaataagaa gctatctggc
    61801 aaaggctcag gtcccaattt catgattcct cggtaagttg cacttgctcc tttttcgacg
    61861 attgtcatac gaccaaattt tcgtacatcg ttgtagcgaa gttggcttcc atcctcaaaa
    61921 aagaaaagta catgcgtatg tttatcgatt ggtgtatttt ctttcgtaaa ttgatatttt
    61981 ccttccattc gcaagtgtga gatcagttca caatgatcga gatgaaaaat taaaaacttc
    62041 cctctacgtc caatggattg gatggtttcg ccaacaagag aagcctcaaa gatgggtgtc
    62101 tctggttgct cgatgatctt gggccacaag acctgaactt tttgaatcgt ttttcctact
    62161 actaaccgtt ccaatccttt acgaacggtt tctacttctg gtaattctgg cattttttct
    62221 tttcaccttc attatattgc tctgtcttaa acaatgttta ttttgcttcg taccacgttt
    62281 ttccccaact gctgtcagtg atcagtggta catgaagaga aacagcttgt tccataactt
    62341 ccttcaccaa tttatctaat gattcaagtt ctttttccgg tacctcgaag acaagttcat
    62401 catgaacttg taacagcatc gtagcttgaa gacctgtttc ttttaatcgt ttatccaatt
    62461 cgatcatcgc gattttcaaa atatcagcag cacttccttg gattggtgta ttgatagctg
    62521 ttcgttccgc aaaagaacgg atattgtaat ttcttgaatt gatgtctggc agatatcgac
    62581 ggcggtgata cagcgtttcg acgtagcctt ggtcttttgc ttcgcgtacg attctttcca
    62641 tatattcttt gacacctggg tatttttcaa agtacgtatc gatgtattgc tgtgctgctt
    62701 ttcggctgat tcctagattt tgtgaaagtc cgtaatcaga gatgccatac acgataccaa
    62761 aattcactgc ttttgcctgc ctacgcatat ttggtgtgac atcctctgcc ttttcgattc
    62821 caaatacacg catcgctgtg ctggcatgga tatcttgacc ttctaggaag gcagctttca
    62881 aatgttcatc atttgagata tgcgcaagta cgcgtaactc gatttgtgaa tagtcagaag
    62941 aataaatcag ccagtctttt tctctaggaa caaatgcctg acgtatcttt ctcccttcat
    63001 ctaatcgaat cgggatgttc tgcaaattag gatcaacaga gcttaatcgt cctgtttggg
    63061 tcaatgtctg gacataacgg gtatgcactt ttccatctga ttggatcact ttcagcagtc
    63121 cctcgacata ggtagattga atttttgcaa tttgacgata ggtgaggata tcctctacga
    63181 ttggtgcttg ttcacgaagc tgttccagta catctacggc tgtggaatat ccagtttttg
    63241 tttttttgat aacgggtaat cccatttttt caaataagat cactcctaat tgctttggtg
    63301 aattcaaatt gaattcttcg ccggcttctt cgtagatttt ttgttcgatc tctcttaagc
    63361 gagcagagaa ctcgcccttc atttcttgta agcgagtcgc atctactttg atcccgctta
    63421 tttccatctc tgccaaaatg atagacaatg gcagttccat tttccggaaa agatcttctt
    63481 ggtttttatc tgccagttct tggctcaatt ttggtgtcaa cgcattgatt gcagctactt
    63541 ttcgcgctaa atgagcaaaa aagagttcct cttcttctgg taaacctttt ttcgcccctt
    63601 tgccgtatac agcttcatct gattggatat cgttgtatcc gtaatgagca gctattcctt
    63661 cgatatctgt gcttttttca ttcgtatcga gaaggtatgc acctagcaag acatcaaaat
    63721 cgattccttt cgtttttcca gtatagcggt ttaaagcaac ataagttcgt ttcgcatcat
    63781 atactttttt cagccgagtc ggatcagtga tccaagaatg gaacgctcgg ttttcgaata
    63841 cagatagatc gttcgtgaca taaattttct tttctgtccc ccacgctact ccgacgattt
    63901 cctctgtatg ataattgtcg cccatcattt ccacgtacaa cgccatatcc gtagtaaaca
    63961 tttcctcttt gaattcatga acgacttcaa acaaaatatc atccattttt acttcttctt
    64021 ctacgatatt caatttagaa aggaactgat tgaaatccat ttctttataa aacggaacga
    64081 gtttatcgag atttttccct tcatacttca atgagtcgat agagacatcc acaggcgctg
    64141 aagtattgat ggtcgccaat tttttcgata agaaagcctg ctctttgtca tttatcagat
    64201 tttctttcat tttgctttgc ttcatttcat cgatatgttc ataaattcct tcgactgagc
    64261 catattgttt caatagcttg atagctgttt tttcaccgat tttagtcaca ccggggatat
    64321 tgtcagaggc atctccggct agtcccttca tgtcaataat ctgttttggc gtcaacccat
    64381 catatttttc cgctacatgt tctggtgtat agctttcgat atcgctgaca ccttttacag
    64441 tgatatccac ttttacttct ttactagcta attgagtcaa atcccgatca cctgaaagca
    64501 cgacgacatc aaattgatct ttgtctactt tttctgccaa agtaccgata atatcatctg
    64561 cttcataatt gggcaattcg taatactgta cccctagtcc tgtcaacaaa tcacgtatat
    64621 atggcatttg ttctttgaat tcgccaggtg ttttggatct tccacctttg tactccgcat
    64681 acatttctgt ccgaaaagtc gtttttcctg catcgaacgc aaccagaaca tgagtcggtt
    64741 gctctttttg cataacattt tcaaacatcg tattgaatgc atagattgca tttgtatgaa
    64801 gaccgtttct atttttgaaa cgttcaagag aattgtgaag cgcaaagaat gcgcgaaaag
    64861 ccacactgtt cccatcaacg agtaataatt tattttttgt catcttctta actccttact
    64921 gtaaagttct tattcattct aacaaagaat cctgcggaat gcgaaaagaa aaagcgggac
    64981 cataccaaat aattcggtat agccccaact gatgaatttt tttctttttt agttgttccg
    65041 tccaaagatt ctcagaaacg ccaagaacaa gttgataaag tccaaataca gcttcaatgc
    65101 catgaataca gcaattccac tatgtgctgt tccgtttgtc tggacataga tattacggat
    65161 gctttggtta tcataagctg taatccctgc aaagatgacg actaacagta gggagatgaa
    65221 cagctcaact acgctacttc tcaaaatgaa gatgttcaag actagtgcaa tgatcactcc
    65281 gattaaagct gcctgtcctg ctcttccaac cccgctaaga tctctttttg ttgttactcc
    65341 gacaacagac atccccatga acatcaaagt agctgtcaag aaagcttgcg ttactgttcc
    65401 aatatcgtag tatgcaagag taaccgctaa tgttatgcca ttcaaaagag agtaggcaat
    65461 aaatccaccg attgctaagc tcggattctt ttgtgctttt acgcctagaa ccattactaa
    65521 aatcagttca acgatccaca gacccacaaa gcctagtgga aaattattga taaaagttgc
    65581 taattgcaaa ggaaataccc aaagagccaa gtaagaagtg actgcactaa gggcaagtcc
    65641 catacctaaa aatccataaa cttttgcata aaaacgattg atacctgaca ttgatgtact
    65701 tggattattc attcaattca ccttcacttt cgattgctat ttccaattca ggaacttgtg
    65761 gcggttggac cataacgaca gcatccaaca cacgatcttc gttcgtattc tccgcttcta
    65821 cagaaatcgt caccacatcc ttcatcttat ctacttttat gacctcaaac tgaaaagtca
    65881 gtgtttcata atggaaaacc ggttcaataa aattgacaga aaaattgaca acatgtgatc
    65941 ctggtccagg aagatgcttt gaaattgcac ttgtgatgat ccccatcagc atcactgatg
    66001 gaacgattgg ctgttgatat tctgtttttt gtgcaaagtc atgttggata tacaaaggat
    66061 ttgcatcatt cgtcaatccc aaatacagca aaagctggtt atcttcaata gattctgtta
    66121 aggataacga gtcgccttcc tcgatttcat ttattgtttt tcctagtttt ctcggcttac
    66181 ctatgttcaa gatagaaacc ctccagttta tttttaatca tacattattg taccaaaagt
    66241 agcgtaaaag gcaagttctg ctcccttgat ttaacagatt cctatagttt acagaggtcg
    66301 tgaaaaagct gtcctgcatc aaataataag aacagccaag aaaaaacagc ttctcatgtt
    66361 tttgatttga ttgggcttat taccgcagat gcaagctttt gaacaccgtt tatcataagt
    66421 tgcaaaaaag ggggcttggc aagatttttg ccactccccc tctttctatt tttataagga
    66481 cactgcagta attgtattac agtacatttt tcgtcgtgct gcttaaaagt ttttcatacg
    66541 cttgttcaaa tttttgaaca tctccagcac ccatgaagat tactactgca tcatgataat
    66601 ctaacaatgg agatacatta ttttctttga tgacttctcc accttttttg attttcgctc
    66661 caaggtcttc aatcttaacg tttccttgtt cttcccgagc cgaaccaaaa atatcgcata
    66721 agtaaacttt atcagctaaa tcaagtgctt ctgcaaattc atccatcaat gcaatcgtac
    66781 gcgtaaacgt atggggttgg aaaactgcaa ttatttcttt atctggatat ttttggcgtg
    66841 cgccatcaat cgttgctttg atctctgcag ggtgatgcgc ataatcatcg actacggtca
    66901 tgtctgctac gatcttttca ctgaatcgac gttttactcc tgggaaagtc agcatttctt
    66961 ctgcaacttc tttcagatcc agtttttcga aataagctac tgcaataaca cctaaagcat
    67021 ttaagatatt gtgtttaccg aatgctggta cagtgaagtg cccaacaaat tcatccccat
    67081 gatagacatc aaaagcagaa ccagaagttg tgcgttcgat gtttcgtgct tggatatcat
    67141 cgttttctgt gacaccataa taataaatcg gcacatttgc ttttaatttg cgtaaataag
    67201 catcgtcacc atacgcaaaa atagcttttt tcacttgtcc agccattgtt tggaaagctg
    67261 taaatacatc gtcgatactt gtataataat ccggatgatc aaaatcgata ttggtcatga
    67321 ttgcatagtc tggtgaatag gcaaggaaat gacggcgata ttcacaggcc tcaaatgaaa
    67381 agaattccgc ttgcggatca ccatgtcctg ttccatctcc aattagataa cttgttggac
    67441 ggacaccact caatacatga gagagcagtc cagtcgtact tgtttttccg tgagagcctg
    67501 ttacagcgat actcgtataa ttttggataa aatgaccaat aaagtcgtga tagcgaatca
    67561 cttccaaacc taattctttt gctcgctgga tttcttcatg tgaatcagga aatgcatttc
    67621 ctgcaatgat cgtcatccct ggttttacat tatctgcgtt aaatggcaga atcgtgatat
    67681 ttgctttttc taaatctctt tgtgtaaaga aatatttttc aatgtctgat ccttggacat
    67741 ttagaccttg ttcatgtaaa acaagagcca aggagctcat tcctgaacct ttgataccaa
    67801 caaaatgata caatttattt tgattttcca tttgaatcct cttctcttat ctagcgtcca
    67861 ttcttttagc tattacattc aacacgctcc tcattatctt ataaacgatt gaaaatttca
    67921 accaaattta tttggaaagg aggattcgct tgctaaaatg gtcaagtacg agaaaaatcc
    67981 gtttttattg actctttaat tctggtgtaa gaagaccaaa cttcctctga tatatcaatg
    68041 tgaggaataa aatcttttcc tttcggacca tagccgttct catcgtcatg gagacgttcc
    68101 atttctccta atactagcgc atggaaatat tccttaggcc attgatgatt cagttctttt
    68161 gctgtaaaga tgatctcttc agaaaagtga aggatttccg gcacaaatgg cacagcattc
    68221 gcgaaaatag tttgttcatt ttgccatact ttctcaaaag ttgctcgagt cctgcgttgc
    68281 agtgtcggat cattcatcaa taagattttt tctggtacag ctcctaagga atgaagaatc
    68341 tccagtgaaa aagtggcgtt ctctccagaa tttgtagact tcgattctat cagaaaagcc
    68401 ttatcgggca aatcatagac ttcttttaga tactgacgac agatttcact ttcactcatg
    68461 ccatcttcaa aatgaaatcc ttgcttttca aaattctcat aaaggattct agtcgcatgc
    68521 cccacccctc cgaccaaaaa caattgatct acttggccat ttaaatacat ttgtgctgct
    68581 ttatcagcta agataggaag acagtttcca gctaaaataa gtgtaggtgc cgtcagttcc
    68641 tcaaaatttc cctcttcagg tgcggctaaa aaatttaata gctgattcca gtcttttaga
    68701 ttcctctgct cacttttttc caaaggcctt ctatcctttc atctgatttt ttgtgcttca
    68761 ttaaagtctt ctctttttgt ctcaatccat ttgatttctc catgacatct tccacaacga
    68821 tatttgcgta catcgattct tctcttgcgg cagatcgtgg cagaacaatc ttggcatcga
    68881 taatactcca tctttatctg tttcttcgtc aaggctggtg catagcgcaa ccctcctgtt
    68941 tttttcaaaa gatacttgaa atccatatct ttatgctgat accctcttcc agctagatga
    69001 agatgataat ggcaaagttc atgtttgatg attcctgcaa tcgtctcttc gtctgactct
    69061 tcaaatagct tcgcattaaa atctaaatga tgatcgttta aatgatagcg cccgccagtt
    69121 gtacgtaagc gtggattgaa cattgcttgg tgcttgaaag gaagctggaa gctttttaat
    69181 gatatctctt ctactaactg ctgcagttct tcttgtgtca atttttgtta ctcctcattg
    69241 ttactcttca taaggggaaa gcatcgtcaa actgattctg ccttttttcg tatcgacttg
    69301 ttctatccaa actgttacga tatctcctac tgacacgaca tctgtaggat gcttgacgaa
    69361 ttttttgctg agtttcgata tatggacaag tccgtcttgc ttcacaccga tatcaacaaa
    69421 tgcgccgaag tcaatgacat tacggaccgt tccagtcaat tccatccctg gtttcaagtc
    69481 ttccatactc aatacatctg tacgtaataa gggcgctggc atctcatcac gcatatctcg
    69541 tccaggttgt gtcagcccag ctaaaatatc ggctaacgtt tcttctccga ctgataggct
    69601 ttcagccagt ttctctacgg ataatcgtgt caatttttct actgcttctt ctgtaccaag
    69661 ctctttttct gaaagttgga ctgaagttaa tatttctttt gcaatagaat aactttcggg
    69721 atggatacct gtgttgtcca aaatgttttt cccaccaggg actcgcaaga atccgattgc
    69781 ttgttcatat gcttttggtc ctaagcgtgg cacttttttc aattgagttc gtgcggtgaa
    69841 ttctccgttt tcttcacgat aaatgacaat gttttgggca gttgttttat tcaatcctga
    69901 tatatgctgc aagagctgcg ggcttgctgt attcacatcc acacctactt ggttaaccgc
    69961 tgtttccaca acaaaatcca attgttcagc caaacgtttt tgagaaacgt catgttggta
    70021 ctggcctact ccgacagctt taggatcgat tttgactaat tcagctaaag gatcttgcag
    70081 gcgacgggca atagaaacag cacttctttc ttcaacttgt agatgcggaa attcttttcg
    70141 tgcaatttcg ctagcggagt aaacagaagc gcctgcttcg ttgacgatag cgtaataggt
    70201 tttatggtcg gcggatttca actgctcagc cacaaaaagt tcagattccc gactggctgt
    70261 accatttccg atagctacca tatctacttg atatttatta attaattgga taaaagctgg
    70321 tccagcagct tctctttttg cttgcgctgc aggtttatga ggataaataa cttcgattgc
    70381 taaaactttc cctgtcgcat ctacgactgc tagtttacat ccagtacgat aagccggatc
    70441 aaatcccagg acaactttac cttttagtgg cggttgcaac aaaagattac ggaggttttc
    70501 gccgaaaata gcgattgctt gctcatctgc cttttcagtc aattcattgc gcagttctcg
    70561 ctcgattgct ggctgaataa agcgtttata actatcctga taggcttctc tgacgaatga
    70621 agaagaagga gaggcaggat ttttgactat ttggcgatcc aagtaagcta aaattgctgc
    70681 ttcatctact tgaaagaata ctttcagaat atcttctttt tctccacgat tagtagctaa
    70741 tattcgatga gaaaccattt tatggacggg ttctgcaaag tcataataca tttcatatac
    70801 gcctttttca tctgcttgct catccttgac attgctgaca tacatacctt tgttatacgt
    70861 ataagaccga atccaagtcc gaaattttgc attatcgctg acttgttcag caataatctc
    70921 atgagcaccg tgcaacgctt cttcggcaga agagacttct ttttctttgt caatatattt
    70981 ttctgcttca gattgcacct ctccatctgt aagctgcatc agccacttcg cgagtggttc
    71041 aagtcctttt tctttagcaa tcgttgcttt tgtccgtcgt ttttgtttgt atggacgata
    71101 caaatcttcc acttgttgca ttttcacggc ttgggtgatt tctgcttcta actcgggagt
    71161 cagtttccct tgttcatcga ttagccgaat cacttcagtt ttacgttttt ctaagttttc
    71221 aagataggca taccgctctt cgatttcacg aatctggact tcatcaagac ttccagtcat
    71281 ttcttttcgg taacgcgcga taaaaggaac agtgtttcct tcatttaaca aattaagtac
    71341 agtagtcaac tgcttaggac gatagtctga aagttctttt tgtacaagct gaatgatcgt
    71401 ctggttcaaa ttttccgtca ttaaaatgcc tcttttctgc taaaaattgc ttctttattc
    71461 tatcatactt tttatcctgg gttaaactac tgaaagaaac aatattctct ataaaaataa
    71521 aaagagccgc aacaaagtca cagctccttc attctttcca ttagtcagat tccatgccaa
    71581 ggtccttcgt tgttctgata ttcaatatct tgaagaatac cttccttgtc aaaaacaacc
    71641 ccatgcaatg accctccgaa tacagcccca ccgtccattc cgattttatg atcctgaatc
    71701 cagaggtctg ttgtctgcat atctccatgg agcattggtg taatcgtatg tccaaataca
    71761 attgtcttac ctgtattgtt tttcccttca tgaaatggtt cccttatcca aataaagtct
    71821 cttagacttg tcttacgcca gtctttcttt gtcaaatcca caccggcatg gacaaaaaga
    71881 taattttccc attcataata caaggggcgc tggactaaaa atttaatcag ttctgaatat
    71941 ctcgattgga tcatcatcga gatttcagtt ggggagtatt cctccgatgc ccctgggtga
    72001 atcaggcttt caaacgtttc ttttccacca tttctcatat aaggcgcata ccagtcctcc
    72061 ggtgattgca gaaattgcaa aaaatattct tcatggtttc cccggaggta aatggctcct
    72121 gtttcttcga ctaattgctt tcctaataaa aaacattctt ttgtacgagg acctcgatca
    72181 ttgagatccc ctaataaaac aagctgatgg atctttggat cataatagtg aataagtttt
    72241 tcaaaaagct catgtttgcc atggacatcc ccaatagcat aaatcaatga tttcatgtgg
    72301 atcaccatcc tcaacgctag tatagcgcat attggaaatg gtcgacaatc aaaactattg
    72361 ttttctattt aattaaagct gtaaaccgca gttctgcttc ttctggagag attccatggt
    72421 cttgaacata gcgattgcgc ggatgtttcg cacactctat gctacatgct cctaaatgtt
    72481 tttcttcatt ttcaatggaa gcaagaatct gacgattgca ctcaggattg ctacaattga
    72541 tatatcgttc acatggtgtt ccgtcaaacc agtcgcgacc gacgatttgt ttatctactt
    72601 ggttgatatc gacagcgatc cgttcatcaa acacatacat cttgccgtcc cataattctc
    72661 cttggacttc aggatcctta ccataagtag caataccgcc atgcagctgt ccgacatctt
    72721 tgaatccttc acggacaagc caaccagaaa acttctcgca acgaatgcca ccagtacagt
    72781 aggtcactac ccgtttttcc ataaattctt ctttgtgctc gcggatccag tctggaagct
    72841 ctcgaaaact gcgtatatct gggcgtacag cccctcgaaa atgtcccaag tcatattcat
    72901 aatcattccg tgcatcaatc accactgtct gttcatctaa aatagcttct ttaaattctt
    72961 gcggtgaaag ataggcacct gtcacttggt ttggatcaat gtcctcttct agctttaaac
    73021 ttacaagctc tggacggtat cttacaaaca tcttcttaaa ggcatgtcct tcgcttggat
    73081 cgatcttgaa catcgtatca gcaaatcgag gatcagcttt cattgctttc atataggcag
    73141 tcgtgttttc aacggttcca gacacagtac catttattcc ttcttcagct actagaatac
    73201 gacctttaag atccaaagat ttgcaaaagg ctaaatgatt tttggcaaat tcctctggtt
    73261 cttcgatttt tgtataattg taataaagca gtacttgata atccatgtat ttcttccttc
    73321 cttcttttcg ttcctaaaca gtataaagaa ggcgttcgca tttgacgatt tatttgcttg
    73381 tgtataatta cgcatggaaa gttttttgtt tacataatat tattatttga attaaaaaga
    73441 cttcgctttc ctgtttcgca cagaa
//
LOCUS       DAFEOM010000007        69093 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.007, whole genome shotgun sequence.
ACCESSION   DAFEOM010000007
VERSION     DAFEOM010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 69093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.007"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(23..391)
                     /locus_tag="LP524_000560"
     CDS             complement(23..391)
                     /locus_tag="LP524_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBL2233637.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            complement(1002..2393)
                     /locus_tag="LP524_000561"
     CDS             complement(1002..2393)
                     /locus_tag="LP524_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HBL2233638.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(2606..2992)
                     /locus_tag="LP524_000562"
     CDS             complement(2606..2992)
                     /locus_tag="LP524_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HBL2233639.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(3048..3671)
                     /locus_tag="LP524_000563"
     CDS             complement(3048..3671)
                     /locus_tag="LP524_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HBL2233640.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(3735..4403)
                     /locus_tag="LP524_000564"
     CDS             complement(3735..4403)
                     /locus_tag="LP524_000564"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBL2233641.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(4415..4855)
                     /locus_tag="LP524_000565"
     CDS             complement(4415..4855)
                     /locus_tag="LP524_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233642.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4914..5585)
                     /locus_tag="LP524_000566"
                     /pseudo
     CDS             complement(4914..5585)
                     /locus_tag="LP524_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5770..6168)
                     /locus_tag="LP524_000567"
     CDS             complement(5770..6168)
                     /locus_tag="LP524_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBL2233643.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(6165..6938)
                     /locus_tag="LP524_000568"
     CDS             complement(6165..6938)
                     /locus_tag="LP524_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBL2233644.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6935..7618)
                     /locus_tag="LP524_000569"
     CDS             complement(6935..7618)
                     /locus_tag="LP524_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBL2233645.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(7785..8672)
                     /locus_tag="LP524_000570"
     CDS             complement(7785..8672)
                     /locus_tag="LP524_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL2233646.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(8669..9538)
                     /locus_tag="LP524_000571"
     CDS             complement(8669..9538)
                     /locus_tag="LP524_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL2233647.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(9675..10961)
                     /locus_tag="LP524_000572"
     CDS             complement(9675..10961)
                     /locus_tag="LP524_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL2233648.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            11234..11812
                     /locus_tag="LP524_000573"
     CDS             11234..11812
                     /locus_tag="LP524_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBL2233649.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            11812..12741
                     /locus_tag="LP524_000574"
     CDS             11812..12741
                     /locus_tag="LP524_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2233650.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALANEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            12738..14474
                     /locus_tag="LP524_000575"
     CDS             12738..14474
                     /locus_tag="LP524_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBL2233651.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(14537..15373)
                     /gene="pheA"
                     /locus_tag="LP524_000576"
     CDS             complement(14537..15373)
                     /gene="pheA"
                     /locus_tag="LP524_000576"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBL2233652.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(15370..15879)
                     /locus_tag="LP524_000577"
     CDS             complement(15370..15879)
                     /locus_tag="LP524_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBL2233653.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(15872..17167)
                     /gene="aroA"
                     /locus_tag="LP524_000578"
     CDS             complement(15872..17167)
                     /gene="aroA"
                     /locus_tag="LP524_000578"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBL2233654.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(17180..18271)
                     /locus_tag="LP524_000579"
     CDS             complement(17180..18271)
                     /locus_tag="LP524_000579"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBL2233655.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(18288..19454)
                     /gene="aroC"
                     /locus_tag="LP524_000580"
     CDS             complement(18288..19454)
                     /gene="aroC"
                     /locus_tag="LP524_000580"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBL2233656.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(19456..20520)
                     /gene="aroB"
                     /locus_tag="LP524_000581"
     CDS             complement(19456..20520)
                     /gene="aroB"
                     /locus_tag="LP524_000581"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBL2233657.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(20553..21575)
                     /gene="aroF"
                     /locus_tag="LP524_000582"
     CDS             complement(20553..21575)
                     /gene="aroF"
                     /locus_tag="LP524_000582"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBL2233658.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(22234..23208)
                     /locus_tag="LP524_000583"
     CDS             complement(22234..23208)
                     /locus_tag="LP524_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HBL2233659.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(23189..25072)
                     /locus_tag="LP524_000584"
     CDS             complement(23189..25072)
                     /locus_tag="LP524_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HBL2233660.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(25090..27315)
                     /locus_tag="LP524_000585"
     CDS             complement(25090..27315)
                     /locus_tag="LP524_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBL2233661.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            27430..28464
                     /locus_tag="LP524_000586"
     CDS             27430..28464
                     /locus_tag="LP524_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL2233662.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(28531..29544)
                     /locus_tag="LP524_000587"
     CDS             complement(28531..29544)
                     /locus_tag="LP524_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL2233663.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKHLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(29622..29810)
                     /locus_tag="LP524_000588"
     CDS             complement(29622..29810)
                     /locus_tag="LP524_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBL2233664.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(30250..30378)
                     /locus_tag="LP524_000589"
     CDS             complement(30250..30378)
                     /locus_tag="LP524_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBL2233665.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(30381..31754)
                     /gene="rlmD"
                     /locus_tag="LP524_000590"
     CDS             complement(30381..31754)
                     /gene="rlmD"
                     /locus_tag="LP524_000590"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBL2233666.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(31865..32920)
                     /locus_tag="LP524_000591"
     CDS             complement(31865..32920)
                     /locus_tag="LP524_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBL2233667.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(32930..34360)
                     /gene="gatB"
                     /locus_tag="LP524_000592"
     CDS             complement(32930..34360)
                     /gene="gatB"
                     /locus_tag="LP524_000592"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HBL2233668.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(34357..35829)
                     /gene="gatA"
                     /locus_tag="LP524_000593"
     CDS             complement(34357..35829)
                     /gene="gatA"
                     /locus_tag="LP524_000593"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HBL2233669.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(35829..36134)
                     /gene="gatC"
                     /locus_tag="LP524_000594"
     CDS             complement(35829..36134)
                     /gene="gatC"
                     /locus_tag="LP524_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HBL2233670.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(36240..38276)
                     /gene="ligA"
                     /locus_tag="LP524_000595"
     CDS             complement(36240..38276)
                     /gene="ligA"
                     /locus_tag="LP524_000595"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBL2233671.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(38293..40527)
                     /gene="pcrA"
                     /locus_tag="LP524_000596"
     CDS             complement(38293..40527)
                     /gene="pcrA"
                     /locus_tag="LP524_000596"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HBL2233672.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            40682..42241
                     /locus_tag="LP524_000597"
     CDS             40682..42241
                     /locus_tag="LP524_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HBL2233673.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(42352..44283)
                     /locus_tag="LP524_000598"
     CDS             complement(42352..44283)
                     /locus_tag="LP524_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL2233674.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(44301..45215)
                     /gene="pfkB"
                     /locus_tag="LP524_000599"
     CDS             complement(44301..45215)
                     /gene="pfkB"
                     /locus_tag="LP524_000599"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBL2233675.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(45212..45964)
                     /locus_tag="LP524_000600"
     CDS             complement(45212..45964)
                     /locus_tag="LP524_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL2233676.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(46123..46332)
                     /locus_tag="LP524_000601"
     CDS             complement(46123..46332)
                     /locus_tag="LP524_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233677.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(46343..47671)
                     /locus_tag="LP524_000602"
     CDS             complement(46343..47671)
                     /locus_tag="LP524_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233678.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            47807..47962
                     /locus_tag="LP524_000603"
     CDS             47807..47962
                     /locus_tag="LP524_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233679.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(48036..48623)
                     /locus_tag="LP524_000604"
     CDS             complement(48036..48623)
                     /locus_tag="LP524_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBL2233680.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(48706..49956)
                     /gene="clpX"
                     /locus_tag="LP524_000605"
     CDS             complement(48706..49956)
                     /gene="clpX"
                     /locus_tag="LP524_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBL2233681.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(50203..51492)
                     /locus_tag="LP524_000606"
     CDS             complement(50203..51492)
                     /locus_tag="LP524_000606"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBL2233682.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(51646..52578)
                     /locus_tag="LP524_000607"
     CDS             complement(51646..52578)
                     /locus_tag="LP524_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233683.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(52735..53394)
                     /locus_tag="LP524_000608"
     CDS             complement(52735..53394)
                     /locus_tag="LP524_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBL2233684.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(53397..54422)
                     /gene="sppA"
                     /locus_tag="LP524_000609"
     CDS             complement(53397..54422)
                     /gene="sppA"
                     /locus_tag="LP524_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBL2233685.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(54435..54725)
                     /locus_tag="LP524_000610"
     CDS             complement(54435..54725)
                     /locus_tag="LP524_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233686.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            55134..56939
                     /gene="glmS"
                     /locus_tag="LP524_000611"
     CDS             55134..56939
                     /gene="glmS"
                     /locus_tag="LP524_000611"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBL2233687.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(57011..58366)
                     /locus_tag="LP524_000612"
     CDS             complement(57011..58366)
                     /locus_tag="LP524_000612"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBL2233688.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(58390..59559)
                     /locus_tag="LP524_000613"
     CDS             complement(58390..59559)
                     /locus_tag="LP524_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233689.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(59556..60443)
                     /locus_tag="LP524_000614"
     CDS             complement(59556..60443)
                     /locus_tag="LP524_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBL2233690.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(60632..64324)
                     /gene="nifJ"
                     /locus_tag="LP524_000615"
     CDS             complement(60632..64324)
                     /gene="nifJ"
                     /locus_tag="LP524_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBL2233691.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            64523..65044
                     /locus_tag="LP524_000616"
     CDS             64523..65044
                     /locus_tag="LP524_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBL2233692.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(65743..66300)
                     /locus_tag="LP524_000617"
     CDS             complement(65743..66300)
                     /locus_tag="LP524_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233693.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLRTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(66479..67090)
                     /locus_tag="LP524_000618"
     CDS             complement(66479..67090)
                     /locus_tag="LP524_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233694.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSARYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(67203..67460)
                     /locus_tag="LP524_000619"
     CDS             complement(67203..67460)
                     /locus_tag="LP524_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233695.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(68057..68335)
                     /locus_tag="LP524_000620"
     CDS             complement(68057..68335)
                     /locus_tag="LP524_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233696.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(68369..68620)
                     /locus_tag="LP524_000621"
     CDS             complement(68369..68620)
                     /locus_tag="LP524_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233697.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT        19329 a        14926 c        12142 g        22696 t
ORIGIN      
        1 aaaaacaaag tctttatttc tattatttac ctgtaaccgc aaagtagttg ccatcgggat
       61 cagcaaagtt aaagacatat tcttctccaa gctgtaccat atcacctaat gtgactgatt
      121 gcgtctgcat ttttttatat agtccaaaga tatctttact gtaaaacatc aaagatgggg
      181 cactagtagc tgtgccaggt gaatgggact caacaaatgc tcgatcatat aacagaaatg
      241 aggtttgact gtcctcaact cctacttcaa ttataagcgt cccatccatt tcttgacgat
      301 ctttttccgc aaagccaact gctctccaaa acgttgcatt tttttctaca tcgtccacat
      361 aaagcatgat ttttatttca ttcgtaaaca tctgtttcct cctaaacgat ctgttctaac
      421 attctcaact ttgtcagttt accataggaa aatgggaaaa tctacgaaag ctctcaacag
      481 aatcgtccta cttaaatacg cttttcttta ataataaata agttggttgt ttttgttatt
      541 tgcgctttca cttttcggaa taaataatgt gtatttatat ggaataaata aaaaaagagg
      601 tcgtgactaa aaacaagtca caacctctgg taaacggtgt tctcctgaca actcctcggt
      661 cacaagacac aatattcaac aaacatgaga agctattttt tgaaattgct ctttgtgact
      721 cagagtgaaa cggcaggctc acgacctctt tcttaaacgg tgttcaaaag cttgcatctg
      781 cggtaataag ctcaaccaaa cgtaaaatat gaggaactat ttttgtttgg ctgttcttat
      841 tacttgatgc agggcagctt tttcacaacc tcttattcac ggtgtttcag aagtagcttc
      901 tcggaaataa gccgaaattt cacaaaaatt tgagaaacta tttttaggaa acttcttctt
      961 atttcttaaa gctgaccact tctatcacaa cctctttttg actatgcatt cacatcgatc
     1021 actttagaat catcattgat gacatcgcga tctaaatctt tgatgcgtgc acttgtcacg
     1081 ataccagcta ccatgctacc gctgacgttt actgctgtac gtgccatatc gattaatggt
     1141 tcaacagaga taactaaccc aacgattgct actggcaagt tcattgcccc taaaacgatc
     1201 agtgaagcaa atgttgcgcc cccaccgact ccagctacac caaaagaact gattgttaca
     1261 attgccacaa gcatcaaaat aaattccaaa ctaaagacat tcactccagc tgctggtgcg
     1321 acgatcgttg ccaacatagc tggataaacg ccagcacagc cgttttgtcc aatagacaaa
     1381 ccaaaacttg ctgagaaatt agctgttgca tcatctactc ctaaagcttt tgtctgagtc
     1441 tcgatattca atggcaaagc tccagcactt gaacgtgaag taaacgcaaa gctcaatacc
     1501 ggaaaggctt ttttgaaata gttgattgga ttgacttttg caccaatcaa aatcagtgag
     1561 tggacaatca aaacagcaag caatgctacg taagaagcaa cgacaaattt acctaaattg
     1621 acgatagctg caaaatcact tgttgccaaa acgtttgtca tcaaagcaaa tactccataa
     1681 ggtgtcaaac gcaaaacaag cgtcacgatc cgcataatga ttttatacag actgtcgact
     1741 aaatctgaaa agaatgctgc ttcttttggc gcttttcttt tcacgcctag ataagcgatt
     1801 ccgacaaatg ccgcgaaaat aacgactcca atcgtacttg ttgcacgact acctgctaaa
     1861 tcagcaaaaa cgtttttagg gataaaggat aaaatctgct gtggaatcga taagttttca
     1921 actgtttcct gacgtgttgc cagttcagag attcgtgctg tttctgcagc tccttgtgtg
     1981 aatttagctc cttggagtcc gaacagcatt gttgaaccaa ttccgatcaa tgctgcaata
     2041 gctgttgttc caagtaaaga agtcaataca gtcgcactaa ttttccctag cttttcagaa
     2101 actttcattt ttgtgaatgc accaatgatc gaaataaaca ctaaaggcat aactagcatt
     2161 tgcaagaatg atacatatcc ttgacctaca atcccgatcc attccattga ttgagtaacg
     2221 actttgtcgt ctacaccaaa gatcaattga agcagaccgc caaagacgat accaactcct
     2281 aatgctgtaa agacgcgtgt tgaaaatttt gtatggcgct tttgcatacg atagaacaca
     2341 tataataatg ccgcaaaaac tagaaccact aaaactgtga tgagtgttgt catttttctc
     2401 ctcctcatat tattaatgac aagtccatcg aaggaagtaa aggttcccga gaatgattct
     2461 tcaaccatcc tcctgaaaaa atttttttca gtgactttta tattttagac gatttttatc
     2521 gaaatgacat tctttttgct tacaattagt aataaaaaaa catacctgtg atagctttaa
     2581 tctatcgcag gtatgttttt actgtctatt cttgtttttc tgattgaaaa ttcaatttta
     2641 ccccaggctg atctaaggcg atctctgtca cctcataaga cattcggcgt acgatatcga
     2701 ataacggtgc tactagctcg tccacttctt cttgtgtgac atcattttga ccttcaattt
     2761 tccgattgag gatatgattg atctgactca cacttccaga taaaacatat gtatcaaaca
     2821 ctaaacggaa ctctaagcgt gctcctacga tgctatgttc tttcggatag ttttcgatcg
     2881 tttcttttaa aggcgagaag cctactttca aatctgtttc tacttcttgg ttcggcaatc
     2941 tcatatcata atgaaatgct tctacaactt ctttttgtct tctgatttcc atataatttt
     3001 atcactcctt tatgaaaatt ataacatatc tcctttgaaa ggggatacta ctttttaatg
     3061 atgctctgca aggtaaggat ttcattcaga tttcctacca cataatctgc cgggatctca
     3121 cccagaaaat gatcgatgtc gtaaagacaa gtagcgactc ctgcattttt ccctgcttcg
     3181 atatctaaac gacgatcgcc gatcatcatc gtgtctgttc tttctaaatg aaaagtatcg
     3241 atcaaataat ttaaagatgc tggatcaggt ttacgaggaa agtcctgatc gatccctact
     3301 acttcctcaa tcagatgtgc caatcgatgt tcttttaaca ggccccatgt tgattctgtc
     3361 aaacggtggg tcaggatgaa atgacgaccg ccgttgtctt ttagcatttc cagtacttgt
     3421 ttcgtttctg gaaatggacg ggaaacttcc gtactttctt tttcatatcg gtgaaataat
     3481 tcattaaact ctctggcatt caagtttgat tcattgatca aataactagt agaatacttt
     3541 ttcattttga agtagatttc ttttttgtcc atactgatac caaaatcttt tagtgcttgc
     3601 catgcaccat ctaccattgc gggatacgta tcaaacaatg taccatcaaa gtcccatata
     3661 taatttttca tgttcctttt gttccattcc tttgcttctt ttttctatct ctcactttta
     3721 gataaaggtg ttctttaaag tatttttttg tccagaattg tcaaatcatc tgctaatgta
     3781 tactggattg gttgtgcatt tgggatatcg atcgtatcca tttgatcttc aggaacatct
     3841 tctaaatatt ttgtcagtgc tcggatgcta ttaccatgac ccacaactag taaatcttgc
     3901 cgatctttta gttttggtgc tagatgatct tgccataagg gaatgatccg attcactgtc
     3961 atttccagac tttctccgta tgggatcaaa tgcggatcca agtgatcata tctgcggtca
     4021 aatctatttt ctgcggttaa cggcggaact tccgtatatc ctctgcgcca tgtttttact
     4081 tgatcttttc cgtatatttc tttcatctta tctttgtttt tacccacaag atccccataa
     4141 tgccgttcat ttagacgcca tgtttttatt ataggaagcc ataactgacc catttcttct
     4201 agtgcgactt gtccggtaag aatcgcacgt ttcaaaacag aggtataaac ggcatcgaat
     4261 tgaagtccgg cttcaagcat tttttgaccg gcaactgctg cttgttcttt tcctttttct
     4321 gttaaggcga catctagcca tcctgtccag taattttcaa aatttgcttc gctttcgcca
     4381 tgacgtaata atactaattt cattcttttc tcccttaaac attcgtttcg aattttatac
     4441 cacatgtttt gcatgtcaca cgtattcgtc cttttccttt aggtgcacgc tgcttttgat
     4501 gacaattcgg gcactcaaaa aaagtataac tttttcgatc cattttgact tgctttttgt
     4561 tttttatctg atgatatttt tctttgactc tatcaagata tttcaaataa cgttgattct
     4621 catttagtct ggggtagatt ttttttgata ggaaacgaaa tatcatccat ccgaaaaaga
     4681 cgatgaacaa caattgtccg attctatagg gcaaccaacg acttagcaaa ctaaagacaa
     4741 gcgcagcgat caatagaccg cgattcaact gatcaaattt cgcatatctt ccctgcataa
     4801 gtttgtgata gttctgctga aatcgtatta gcctttctaa ccatgtttgc atcatattat
     4861 ctcctccatc atctaatatt tattatgaca aaaaaatcca aaaagaaaaa gccttaattg
     4921 gccttttcca ttgcaacttg gtgaaggaca ttccgatttc tggtaaagtc aatcacaatc
     4981 actttctttt tatcttctct gttgactact cgaaatgtgt ccgtcaatgg aagaaacagc
     5041 ttcttagatg gtagctgttg catacgtata aaacgatgga tcatcgtaat caattgagga
     5101 aattgaatag acgtgtgaag cagacctgga tattttttca acagctgtgt cgttttgatc
     5161 agattccgtt ttttactgat cactttttaa aagcttttta gaatcttttg ctgacgtcta
     5221 aaaatagaaa aagaattttc gtcatctatt tgataacgca tttgtttttg cagttctgct
     5281 gcagtcagat ggatcttccc atcgtttgta tcgatcatta aaccttctgg aaacagttca
     5341 accactgctc gatcccaaga ttcaagagaa agctcgatat aatcttttac tggtaattcc
     5401 aattctacct taaaaaagtg caaaacagcc gacagccctt ctgcttgata gatttctcct
     5461 aatgttagcc cctctagatg gaactctggt gaaaaatgca gcaatgtttt attttcctgc
     5521 aattgaacat atagaaatgt agatcgctca atcagagtat cttttaaaac aactaaaaaa
     5581 ttcatttttc tccccctttt ttcatcattt ttaaaatagc atattttgaa aagaaagtct
     5641 gatgcttaga gtagaatttc ataaaaattt actatttcat aagtattcac gagaaaaata
     5701 caaacaaaaa agcggaaata acgctcatcc gccttcttgt ttgtgtttat ttcctcttca
     5761 aacgcttgtc tatgattcag acttttctct atcgttattt tcatctaaaa gttcttgcat
     5821 tccgataatt tctcgatatt gatgaatctt agtgatacca atgactttgt ctacatcaat
     5881 actaaaacaa ataccattac ctggaatatc gatgttgatg ccttgaccaa tcgcatccat
     5941 tactttattc gtcaaactat cgggtactaa tgtcaacaca atatcttttt ctggcgaaat
     6001 gtccaaacct aaaaatttcc ctgaatcatg tacaccagaa cctcttccat gtaagatcgt
     6061 tccgcctgct gcacctgctt cttttgaaaa gtcgataaca tcttcgccca ttcctccgtc
     6121 aacgatcgtc acaatcatgt gaagattcaa tgctacaaga tctttcattt gctttccccc
     6181 aatttttgca atctttcttt ttcgattctg tctttttcat cttttcgttt ataaatatat
     6241 cctagtacta aagtggataa tattggtgtc aatgcaatca ttgcaaccac accaaacccg
     6301 tcaatgatcg gatctcttcc ttcaatttgt gcagcgattg ccacgctcat tgataaaata
     6361 aatgtcgaac acatcgggcc cgttgctact ccaccgttat caaaagccat tgctaaaaag
     6421 atcttgtcca cttttcgtcc taaaataaat gctagcccgt atcctgggat caagaaataa
     6481 taaagagaga atccgacgag caaacgccac atagacaatc ctactgctgc gccgatccca
     6541 attgaaatta ccatcagcat gattttcgat cggattctcc cctcgctcaa ttcttcaacc
     6601 tgtttgatca ttacatgaat cgccggctca gcaaacccta ctaagaaccc cattaaaaag
     6661 ccaaggggaa ccagtatata attgtaaggc aatcccgcaa tagcactgcc taaatgctgt
     6721 cctaccggta cataagccat attgactccg tgcaggaaaa ggatcaaccc aatagtcgtt
     6781 atcaagaaac ctttcataat gtcaaagaaa cgctttttgc gcatacgaaa actaatgata
     6841 ttcattaaaa caaataggat tacgataggt gccactgcca aaatgacttc catggtaacg
     6901 atttcgattc catctatcac ttgctgccag aaactcattg gaagatcacc cccaaaatca
     6961 tcacggcaag aatcggccct aacgaagcaa taccgaccat tccaaaactg tcattttcat
     7021 ttttcttttg tttgatcata ctagtcatcc ctccggcaag ggccaagata aatggaacag
     7081 ttactggacc agtagtcact ccccccgaat caaaagcaat cggcataaat tcggaactgg
     7141 tgaaaaatga ggcgatcaaa acgcctatgt agcctatagc catcaattga taataggata
     7201 gcttgaatac gacccgaagc agcgcaaagg ctagaaatac tccagtgccg acgctgacga
     7261 tcccaatcag caacactctg ccaatctctc cgtcagatat atcatatact tgttgtccca
     7321 aaacttgcac ggaaggttca gcaatcgtga tcacgatccc gatagcaaat cccaaactga
     7381 ccaaaacggt caaacttttc tttttgatca tatatttccc tactagatct ccgacttcca
     7441 tcatagaata atctgctcca aacaaaaaga gcgtcattcc gatcatcatg attgccgcac
     7501 caaccagaaa tgaagcgagg tcttctcctt ctaacggtgc aaaaacaaac gttaaaatga
     7561 caatcaggac agtcatcggc aatatagcag caatcacttc tttaaaattt tttagcatgt
     7621 atcctcctcc tttactcttt aagtttacct taaaatgaaa gagttttcga gcacaaactt
     7681 aaaacgttat catttaattt ttctcaacat aaaataacac acgtcccaaa agtatctttt
     7741 tacttggaaa aatgactttg aaggaacgcg cttattgtgt ttttttattt tacagagccg
     7801 atcattcctt gaacgaattg cttttgcaaa acaaaaaata cgatggcagt tggtaaagta
     7861 gcgatgacta atccagccat gatcattcca tagtctggtg tataggaagc acccatagca
     7921 gataaaacaa gcggaaccgt ccgtttatca ggagattgca aagctactaa gggccataaa
     7981 taattgttcc agctactcat gaatgtgatg attgcagccg cggcataggt atttttagcc
     8041 gtcggcatat acactctaaa aaaaatactt agttctttta gaccgtctaa tcgagcggct
     8101 tctactagat cttttggaaa agatttaaca ttttgtcgaa agaagaagat caaaaaagca
     8161 gtgctaacag atggcaggat gacaaccaag aaactgttga ttcccaaaac cgttccgttc
     8221 aattgtgaaa acattcgata taaagggatc atcaatgccg caaaaggaac catcatcgat
     8281 agcagtagaa tattgaaaat taggtctttt cgtttacttt gataaatctc aaatccatat
     8341 cctgccattg acgaaatcag caatgcaaac acagtggtca ccaaagcgat catcgctgaa
     8401 ttccaaaggg aacgagtaaa attcagctca ttggaaaaca aattttgaaa attgatgatc
     8461 aagttactgc caatctttat ttttcccgat gtgatatcca cgggtgtatt cgtcattccc
     8521 aaaaccatcc agacaaaagg gaaaatggaa ataaatgata ggatgcttaa tgctgtatac
     8581 tttaatactg acccaattgt cgaacgaagt tctaatgctt gttttttgac gttggggcgt
     8641 tcgattgttt ctttttctac catatttttc atcgactttc tcctcctact ttcgtttgga
     8701 ttatcgacaa tatcacaata aaggaaacaa taacaaagga aactgctgcc gcatatccaa
     8761 aattaggcgt gaatttataa ctcaaattat agatgtattg agatagggtg gtcgttgcat
     8821 ttgctggtcc cccgccagta atattttgta cttcatcaaa aagctgcaat gtcccgatcg
     8881 tcgaagtaat ggaagtaaac aaaatgattg gttttaaatt tgggatcgtc actttgataa
     8941 actgctgcca tttagaagca ccgtcaatag atgctgcttc ataaatctcc gaatcaatat
     9001 tttgcatgcc tgaaaggaaa aaaatcatat tgtatccagt ccaacgccaa gtgatcgaaa
     9061 gaatgatcag aactctcgcc caaattggat gagtcaacca gccaatcggt gtgtcaatca
     9121 taccaatgcc caacaacaag ttgtttacaa ggccagattg agaaaataga cttttcatga
     9181 tcagagaata actgaccatc gaagtgatac atggaagaaa gatagcggtt cgaaacaacc
     9241 ctttatattt caacttttta tcatttaaaa ggttggaaat aaaaagtgct agaaacaaca
     9301 tgatcggtac ttgtacaagt aaatacagga atgtattaaa aaaagctttt ttaaaagttg
     9361 catctgtcaa catccgctga taattaccta acccgttaaa tgtcatattg acagggggac
     9421 cactttgaaa agacgtgatc aaagccaaaa ccatcggaat aaaaaccatg attgatatca
     9481 aaatgactgg caagattaaa aaagcatgcc cgtttttgaa taaatctttc tgcttcattc
     9541 ttttccctcc aattctatca ttgattatct acatgaaaag acaagaaaga caatatctgt
     9601 aaggcagacg tgatcttttt tgtcttttcg tttgtctgat gaaaaagcga agaaattcac
     9661 ttttttacca ccgatcaatt agccaattgt gcttctactt gtttttgtgt atctgctagt
     9721 accttgtctg tctcttctcc attgatgatt cgatgaagag cttcagctac gactgcttca
     9781 attgcatagg tctcatgtcc gtaattcact tctggtattt cagatgtcca tgttgaaaaa
     9841 tcgtcaaaga tcttttgacc actgtaaaat tcagaagctt cttgataagc ttcttgttca
     9901 ttcgcgctaa gcatcgtaga tactagtccg atttctttcg caagagtgcc catcagttct
     9961 tcattcgaag caaacgttgc ttctaggaaa tctttcgctg aatcttctcc agctactcct
    10021 ttgatcacat accagccagc gccgccaagg ttagaagctt gcgctttttg cagattttct
    10081 ggaagtgcag gtattggtgc aatcttccat tttccagatt ggttttctgc tccttggatc
    10141 gttgaagaat accaagcgcc agttgggcta gaagctactg ctcctgattg tatcgcagtt
    10201 actccagcat tccagtcaga ggtttgttcg actagatctt ctttcaaaag agtggcaaac
    10261 aattcaagac catactttaa agatgcattg tcttggatcg tgactgtctc cccatcttca
    10321 gatgtatacc attctcctgc ctgttgcata atcatcctta ctcgaccaag gtcagaagga
    10381 ttgacttcag cgattttctt tcccgtcttt tctttgacat ctcgcgctac ttgaatatac
    10441 tcgtcccact tcagattctc catatcctct tctgtataac ctgcttctgc catcagatcc
    10501 gtacgataga agtttgctgt tacgcccgaa tcgaaaggaa caccgtatat tttatctcct
    10561 actttgttta ccgcaaattt atacggtgca aaatgatctt cctttacaat agcagacaaa
    10621 ttagcgaatg catctggata agaagtcaaa tagccttgta tcctataatc ttcaatcaag
    10681 acgatattgg gcaatccttc tgtattcccg ctcgctaaag cggtgttcag tttctgaacg
    10741 atatcatcct gagacatcgt aacaacttct atctctgttt cttcgttctc ataaactttt
    10801 tttgcttcat tcactgcttt gatattgaat gtctcatccc acgcccagac agtgactttt
    10861 tcttctttgt ctgctgtact actagcactt ccgcatcctg ttaagaaacc tgatgctgcc
    10921 gctaaacaaa cgactccagc aattaacttt ctgatgttca tttttatccg ctccttttcc
    10981 atccattagt tgtttacgtt tgctacattc atcataccaa aatttgaaaa cggtttcttt
    11041 attattcaga tacttcaaaa atttctccta aaataataaa aaaatacaaa tcaactatga
    11101 caaaaggaat aatcttagca tctagaccaa agatcgaaat ggtgaggttt ctttaaaata
    11161 aaaatatcct tgcattttct cctagaaata agtattttta aattacaaga ctattcttaa
    11221 ggcgggtaaa aaaatgccaa tacgcgtaat tcttatcgat gatgaacaga cgattttaga
    11281 tggaatgaaa cagttgatca attggtctga gcacgggttt caattaatag ggactttttc
    11341 cagtccgctg aaagcgcttg atttttcttt ggtacatgca atcgatatcg tggtcacaga
    11401 tgtaatgatg cctgaattaa atggaatcga attatcgcgg ctattaaaac agcaacaacc
    11461 tgaaacacag atccttatcc tctctagcta tgatgaattt gatatggtta aagattcttt
    11521 taaagaaggc gtcgctgatt atcttttaaa gcctcgattg agtcctgatt tttttctaaa
    11581 ttccttaact gccgtttcac aaaaaatcaa aaaaaagccc aacaaaagcc caatcaccaa
    11641 tcgttatgag cagatctgcg agaatctttc acagcagatt gctggacaaa ctcgtccatt
    11701 gcttgaccct cttctttttg aacacacctt gtttttctgt tatataccga aaaacggcca
    11761 ttcagttcaa aacaaggctc taaacaaaaa cagtcgatca cccattcatt gatggatagc
    11821 gacgattcgt ttactattta tcctgtttct accttttcgg aagaatccgg ctatgtaatc
    11881 aatactgatt ccaaagatac acttaggaag tttgttcaaa agtttcgtca agaagttttt
    11941 ccaggtgatt tttttgttgt ttctgattct tttgcaatcc aagatatcaa tgaaatattc
    12001 caagagatca gaaattgcac caaaggacaa cgtttctatc ataagggaaa gaaccttgct
    12061 tatcagcatg aacttcttcc gttattaacc aaccacgagc agcaagcaag tagttattta
    12121 acaaaaatcg ttcataaaga ctacgcttta gctgtcaaag aacttgagaa tttcttcacg
    12181 acgatattgg aacagcctat tgatccagct tatttgaaac atgaggcaat caatcgtttc
    12241 tatgcattac tcaacgcttt ggctaacgag catgttggga atgaagaact ttctttgctc
    12301 aagatcgccc ttccttcttt attagccgat gctgagtatt tcgaagattt ttctattctt
    12361 gtcaaagaaa cacttgagaa actcacccac ttttctgcaa atgcaaaaaa agacaatttc
    12421 gatatcttga ctttgatcta cgagttcgtc caggaaaatt atgagaaaga aatcaattta
    12481 caacttttat ccgaaaaata tcatttttca tatagctatc tctcagccat ctttactgaa
    12541 aaatttggta tcaacttttc aaaatatcta aaaaaagtcc gtatcagtaa agccaagctc
    12601 tttttgacgg aaacacggct tactttgacg gaaatctgtg agaaagtcgg ctataccgaa
    12661 ctgggttatt tctccagagt ttttaaagaa gagacaggaa tgacaccttc tcaataccgt
    12721 aaaggaactt ttgtaaaatg agaaaaaaaa tcaaagagca tgaactcttt gccaaacttc
    12781 tgttcatttt gttcatcggt ctttttctgc aagccatcgt catttctttg tttatttatc
    12841 atcgatcaag ggatgcctat atccagctgt tcaatcaatc gaatgatgtg gtactaaaaa
    12901 agattcaatc tgaatttgaa agtttgaatg atacaatcga gaatacatta gccgcttttg
    12961 attctaatcc agctgtcaaa agctattttt ccaatgatcc agcccaacat atggaacagc
    13021 ttcagcaact aagaacaatc caaaaaatga atgactctct ttcaaaaatc catccaatga
    13081 tcgattacga tgtattgatc tttggagaaa atggaagaac ttttgtcgga aacgacatgc
    13141 tcactgccgt atctgccgat tctttttttc aatctgcgat tgctcagaga gtgaatgaac
    13201 gtgctgctga tacacaaatg ctttttgcac accacggatt gactcttcgc gataagaaag
    13261 cacccagcgt cttctttgtc aaaaaactga aaaatacatt gaatcacgtt tacggatatg
    13321 ctgttttatc gatctcatcc aagcaactag cgaatctttt tcaatctgta gtaaatcctg
    13381 agatatcgaa gatctccttc atgaacgaag aacagacgat catcgcatca aatgaagaaa
    13441 atacaattgg gagccgatca atgattttca aaaaactaac agtaggtgaa acaacgattt
    13501 caaatggaca gcagttgacc cgtcttcctc tgtaccgcca ggattgtaca ttactcagtc
    13561 aagtcgatct tcattcttta tctaaccaaa tgggcgtgat ttttccgatt attttgtata
    13621 atatcgtgac acttgctttt gctggctcat tggtatttat gtatttggat cgacacacaa
    13681 aatcgatcta ccgcttgatt gattcgctaa aacggctaga ggaagatggc ttaaaagcta
    13741 ctgttcctgt acaaggaagc catgaagtcc aattgctagg gaatacgatc aatcaattgc
    13801 taagtaatgc tcagcatcat tatgaacaga cgatccaaga cgaaaagaag aaacgttcgc
    13861 ttgaaatcca aactatgcaa gcacagatac agccccattt tatttataat acgctgacta
    13921 gcttgaaatt tttgatttgg cagaaaaaaa cagatgaagc agttcaagga atcgacagtt
    13981 tcattacttt actaagaagt acgattggga aaaaagaaga agtgattcca gtcaaggaag
    14041 aaatcaaaag cgtacaaagt tacatcgatc ttttgagtat gcgatatggc gaaagaatct
    14101 cggctaaaat aatgattcct gatgaactgc agtcattatc tatgccgaat atgatccttc
    14161 aaccaattat cgaaaacgcc tatcttcatg cttttcacaa taaaacgcaa ggattcatca
    14221 cggtgtacgg caaaacaaaa aatcagcttc ttttattcga agtgattgat aacggcgatg
    14281 gcttcgatac tgaaaatacc aaacaaaaaa atgatcattt ttcaggaatc ggcatcagcc
    14341 atgtagatga gcggatacgc ctaatgtatg gcgatgcata tggcttaacg atccaatcag
    14401 tgattgggac aggaacgact gtaacgattt gtctaccgct tcttcctaaa aatcaatcag
    14461 aatatcaaaa ataaagaata gccaagcgct gattttttta ttttaagtta aataaatcaa
    14521 tcagtattct tgactttcat ctacgcgtct cttctactgt cagcactgga taacagccca
    14581 atatttgact ttctcctccg agcatcttga tttcttggag cgcattctct actaatgtca
    14641 ttggacggtc caacaacaaa tcgatcacga aaaaatattc tcccaagcta gttttcaatg
    14701 gtcgggattc aatcttactt aagttgattt cccgccagcc aaaagctgcc agcattttat
    14761 gaagcatccc tggccgatta gcaggtagtg tcaagatgac actcattttt actggagctg
    14821 actgctggct ggtcatcttt cggtcaccga cgatccaaaa acgtgtctgg ttcaactcat
    14881 tatcttggat attctcagct aagatctcca atccaacatg ctgtgctgtt tctaatgaag
    14941 caatcgctgc tgcgtcttct ttcggatgtt ctgctacata cattgctgca gcagttgttg
    15001 attctgttgc tactaaagga acattaggat aatgcgtttc taaaaactgt tgtgattgtg
    15061 ctagtgcttg tggatgtgaa aggatttttg taatctttgt tgctggattg cctaaaagct
    15121 gctgcttgat aggaagaacg atttcacttt taacttcgac ttctggatgt ttggaaagaa
    15181 gatcgattgt gtgatgaaca gctccttcca atgaattctc tacaggaacg acagccagat
    15241 cgacttgttt gcgaaacaaa gcgcgcaaac aaacagggat ggaagcatac gcagtcaatt
    15301 gttctgtagg aaatagctga caagccgcct gataagtgaa tgaactttct ggccctagat
    15361 agctgacgtt catatgcttt tgatcctttc tataatggta tcgatgatct tttctggtgt
    15421 ttgattggtc gtgttgattc gatgtgtcgc actttcttca taccaagaaa gtcgttgtgc
    15481 caataatgct tttatttctt ctcttgtttt tccaagagct aaaggtcgaa tagacgtttc
    15541 atcttgttgg atacgatcaa ctagacagtc cgcttctgcc tctaagtaaa gaacaatcgg
    15601 ctgtgttttt aaaaaacgcc gattttctgc actttggaca atccctccac cagtcgcgat
    15661 aattttgtct tcatttgaca gctttcttaa aatatctgac tccacttttc gaaagctttt
    15721 ttcaccgtaa tagtgaaaat attcggcaat cgacaattgg atatgttcct caatctttgt
    15781 atctaaatct acatactctt tttgtaaaca ttgagctaac cctttaccga ttgttgtttt
    15841 tccagcgccc atgaaaccaa tcagcaaaat gctagccatg agcttctcct cctgacacaa
    15901 gttctgctaa atcgtcaaaa aatgccggat aagagattga aacagcttca gctccttcta
    15961 gttcgcaagg tccatcagct atcaaagcag ctacttgcag catcataccg attcgatgat
    16021 cgcctctgct ggacactttt gcgccatgca gaggtgtcgg cccatggatg atcagaccgt
    16081 ctgctgtcgc ttttatcttt gctcccattt tctgtaattc ttctgccacc gcatcaattc
    16141 tgttggtttc ttttactttt aattcttctg cgtctttgat gatcgtgatt ccttctgctt
    16201 gagtagccac taaagctaga atgggcaatt catcaatcag acgcggaatg atttctccac
    16261 caatcgttgc tttcttcaaa tgactcgtct tgacacttaa atcagccgat tggttctgtt
    16321 cattgtgatt cgtctgggta atcgctgcgc ccatctcctc caacacatct aagataccag
    16381 ttcttgttgg attgaccccg acatttttta acaacagttg gctttccgga acaagaagtc
    16441 ctgctgctag aaagaaagca gctgatgaaa tatcacctgg aacagttatc tgttgaccag
    16501 ttaatttttg cggtccggtc actatgatcg ttttatcttc cactgtgatc cttccgccaa
    16561 attgacgaat catttcttcc gtatggttac gggaacgttc tttttcaacg attacggatg
    16621 tgccttctgc ttgtaatgcc gcaaacagca aagcagattt gacttgcgca ctggctacag
    16681 gcatattgta tcggataggt gataaagagc gggttcctgt cacagtgatt ggtagatact
    16741 ggtcattttc ttctccttgc aagtcagcgc ccatctcgcg taaaggttcc atcactcgcc
    16801 ccattggacg tttagacaac gaagcatccc cgaacagagt agtcgtaaaa ggctgtccag
    16861 ccaaaatacc catcaataaa cgtatggtcg taccggagtt tcccatgtca attgattcgg
    16921 ttggttggac aaatgagtgg ctaccgcgac catgaatctt gatcacctct tcctcttctt
    16981 cgatctcgac acctaattgt tggaaggcat gtaaagtact cagacaatct tctgcgcgca
    17041 aaaaattttg tatagtcgtc gttccttcag caattgctcc aaacatgatg ctgcgatggg
    17101 aaattgattt atctgctggt atcctaacag tccctcttaa tccatgtatt tgttgtaata
    17161 attgcattta tccagcctcc tatttgatcc agcaatgata actggtatta gacataatga
    17221 ttctttttgc ttgtaacaaa tcctgttggt ttttaaaact gatttgcagt attcctaaaa
    17281 tatcttccct ggtctcttgg atcttcaaat taatcaaaga aactcccgct tttccgatca
    17341 gtccagttac ttctgcaata acgccaggtt catctggcac atcgataaat aaatcaaaaa
    17401 aggcaggaat cgcgccttgc ttatggacag gcaactggtc tcgactttct tttgcccgat
    17461 tgaaaaaaga aaagatttcc gattggttct cagtcatgat ccattctgct atttgtgtca
    17521 tttcttgttg ccaatctgcc aatcgttcca ataatatctg cttattgctc aacaaaatat
    17581 ctgtccacat gacaggatct gaggaagcga tcctagtgat atctcgaaag cctcctgctg
    17641 ccagttgttt ggctcgcgga tactcttgat tgaattgttc tgcctgattg actaagccag
    17701 ctgccagaat atgtggaaaa tgacttagca ttcctgttat acggtcatgt tctcttgctg
    17761 taagagttac atattttgct ttggttccgc aaaatagttc ttgcagtttt tcaacatctt
    17821 ttttcgtctt ttccaaaggt gtaaagatgt aataggcatt ttcaaatagt ttagcatctg
    17881 ctgcctgtat acctgattta tgagaaccag ccattggatg tccaccgaca aaccgaagat
    17941 ttttctgctc agcggatgtc acgacttgct gcttcgtact gctgacatcc gtaatgatga
    18001 cgtgatcaga caagttgatt ctttcaagga catcaagata agctaaggtt gtacggacgg
    18061 gaacggctaa taagataacg tctgcttgtt ctgcacctgt ttcaaaatca cttgcaacgt
    18121 aatcaacgat ctgctgttct actgcttgct cacgggtttt tgactgacta tcccaaccaa
    18181 ctactatcag ttctggattg gtctgacgaa tacaaagtgc catagaagca ccaatcagtc
    18241 caagccccac gataaaaaca tgttgtgcca tcttatttgc ccccttttta gtaatgtttc
    18301 aagtagtcac ggtactcctt cactgcagct ttcaactcaa caagcgaatc acttgggaat
    18361 tttgacaata gctctgtcgc taccacagtg gctacaacat tttccgcaac gacgctagct
    18421 gcaggaactg ctgtactatc cgaacgttct acacttgctt tgtaaggttc ttttgtatcg
    18481 atatcaacac tttgaagtgg cttgtacaat gtaggaatgg gtttcatgac cccgcgaatg
    18541 acaagtggtt cgccattggt cattccgcct tcgaagccgc ctagattatt ggtacgtcgg
    18601 aaatagcctc tttcttcttc ccataggatc tcatccatca cttctgagcc tggtaatgaa
    18661 ccagaagtga atccgacccc aaattctacg cctttaaagg cattgatact aaccacagct
    18721 tgtgcgattt tggcatctag cttcgtgtcc cattgtacgt agcttcctaa tccagctgga
    18781 acgcctccaa cgagcacttc tacaattcct ccgatcgtat ctccattttt ctttgtctga
    18841 tcgatcaaat cttttattct ttgctcaact gctggatcaa caactcgtac ttctgacgct
    18901 tcagaaattt ctttgatttc ttgaatcgag atatttccag gtatttctgc ttggattcca
    18961 ccaagacaaa tcacgtgtcc tgctacgatg atgtcaagtt cttccagaat tttcttggca
    19021 attgcgccga ttgctacacg catggtcgtc tctcttgcag aggaccgttc aagcacattc
    19081 cgaagatctc gatgatggta tttcataccg cctaccaaat ccgcgtgtcc tggacgcgga
    19141 cgtgcaactc gccgcagtga ttggtctttt tcaggcactt ctgctacaga catgacagtt
    19201 gtccaatttt tccaatcttt attttctacc actaaagtga caggtgaacc aattgttttg
    19261 ccgtgacgga tgccagacgt gatccgaaca cgatcttttt cgatcttcat ccttcctcct
    19321 ctgccgtaac cagcttgtct gcgagcgagt tcgtgattga tttcttcttc tgacagaggt
    19381 aatcctgctg gtagtccttc aataatggct gttaattctg gtccatggga ttctcccgca
    19441 gtcatataac gcatcttaca gtcctcctaa gtaatctttg aatgtctctg tgggaacagg
    19501 aacgattttt gcttgcccaa tcttttctag caagatgata ttcaattggc ttcctcttgc
    19561 tttcttatca tgggttatcg cgtggaataa ttcatctgga tgacttagat cgtacgtaat
    19621 cggcagatgg aatttttgaa gtatcctttc taaagcagct gtcgatccag ttggagacaa
    19681 gccgattttt tctgcatgag aagtgatggc taccattcca atagctactc cttctccatg
    19741 actgactacc ccatatcctg ctgtattttc aattgcatgg ccaattgtat gaccaaaatt
    19801 taaaataagt cgatttcctt ggtcaaagac atcttcttcc actacttttc gtttgatttc
    19861 caacgtagca gtaatgattg gaacagcatg ttccagaaga tctttttcgt ctttcagctc
    19921 gttcagctgt ttccaaagcg ttgcatctgc tatagctgct gatttgatga tttcagcgat
    19981 tccttctcgt aaacgacgta ccggcaaagt ttttagaacc tcaggatcaa tcaatacacc
    20041 agctggctgg ctaaatgttc cgacaaggtt tttcgctttg tttgtattga ctgctgtctt
    20101 cccgccaata ctagaatcta cttgtgcaag tagcgtagtc ggaatttgaa cgaaatggat
    20161 cccacgcatg aacgtcgagg ccacgaatgc tgctagatca cccaccattc ctccaccaag
    20221 tgcaagtatc ccatcgctgc gggtaaaatc attatctgct aaaaaatcgt ataactcagc
    20281 tgcttgttct aaagatttac tggtctcacc cgccggtacg attgaaagga ctggttcaaa
    20341 attagcttgt ctcaattgga acatgatttt ttctgcatat agtggagcaa cgtttgtatc
    20401 agtgatcaca gctacttttt gaggtctcca aatcgtttga agccatgatc cagcctgttc
    20461 tagtgcgcct ttttcaatat gaatatcgta tttttggtta tcaacatgta cagtaagcat
    20521 tcgtttcact tctttctttt aaaaagctgg aattaatcga gtgtctgaat cttttccatt
    20581 gcttctttca tgatcgcaac ttctttcatc atgcgtgagt aagtcttttc attcaacgat
    20641 tggggaccgt ctgaccaagc atttgcagga tcaggatgga tctctacgat catgccatct
    20701 gctccgcttg ccacacccgc acgagccatt ggtggtacaa gttcccaaat tcctgtacca
    20761 tgacttggat caacaatcac agggaaatga gttaattttt tcatcaatgg gacagcactt
    20821 aaatcaaacg tattacgtgt cgctgtttcg taagtccgaa tcccacgttc gataaaaatg
    20881 acaggagatt tgtcttcgac tgcaatgtat tctgcagcat taagccattc attgatcgta
    20941 ccggaaattc cacgtttcaa tccaacaggt ttacctgttt taccgacagc agatagcagt
    21001 ttaaaatttt gcatattacg tgcgccaatc tgtaggatat cgctgtactc agccaccata
    21061 tcgatatgcc cttcatccat cacttcagta atcactttca ttccaaattc atcagccgct
    21121 tgacggatgt atttcaatcc ttcttcttct aaaccttgga aagcatatgg agaagtacgt
    21181 ggtttgaagg caccacctcg cagaattttt gcgcccccgg ctttggccat tcgcgcacat
    21241 tctctgattt ggtctaatcc ttcgatagaa caaggaccag ccatcgtcac gaaactgccg
    21301 tcaccaattt ttactccgtc tacatccacg actgtattgc caggatggaa ttctcgagaa
    21361 gtcaatttgt atgtgtttaa aatacgcaca gcattttcta ctccttcata gcgtgtaaat
    21421 gctccttctt gtaaacttcg cgtatcacct tttagtccaa tcactactcg ttctgttcct
    21481 tgatcgattg agactttgaa tccttctttt tcgatatgat cgacgacttt ttttatttgt
    21541 tcttccgttg cttctttttt cataattact atcattgttt ttcccgcttt cttttgttta
    21601 cttatctatc ttggctgatt ttctctactt tttcaaggtg gttttacatt aattcctact
    21661 aaatgaccgt gtcctgttgt tctcattttc gctccttctt tcctacttat gacccagata
    21721 atcaacaaaa aaagatgtcc ctgtaggaaa ttgcctctac agtgaacatc ttcaacgagc
    21781 cttaaaaagc gctgcaaaaa tataaaatag ccactataga tgaatttctc tacgtggctg
    21841 taagtcgtgt ttaaaaatct ttagccatca tagatacaaa cctggtttgt ttgtacccac
    21901 gaagtcatga atacaaacct atctaaaata gctaaagtaa tacgtattca gttttgtaag
    21961 ttttctagat ttcataaaaa atcgctccaa actgatgtaa gaattgtacc tagtatagac
    22021 ataaaaaaat cgactgtcaa tcatttttat gaaacttttt ttaattttct gatagttaac
    22081 tttgtttttc agaaaaataa caattggttt tattgaagaa aatagtcatg aacaaaagta
    22141 tctgagaact tttttactct cttgttcatg acatatagaa taaagcagct ttgtcttgta
    22201 aactccgttt ttcaaataat aatctaaaga ttgctactga ataataaagc cgatttcatg
    22261 gtcctcttcg ctgctgattc ggtaaggtga ctcaacatcc gctccccagc tattgatacc
    22321 cccgactcct cttacttttg caaaaatcgt aagaaccgtt cgtctttctg gcggtagttc
    22381 ttctatatgt gttgcctgtt ccagttcttc tgctttgaat ggtaaagcgg aaaaagcaaa
    22441 tggcgtatcc tgctgttcaa atcgcaacga aaaatcttct tgatctttat ccgtattatc
    22501 caacgtttga tttcttgtta gttctaccca tttcgtttcc atatgcatgc cgcattcttg
    22561 gggaacaagg tatggggaaa cggggagtcc tgccacttta taaatacctt cgacaccacc
    22621 agccatccga tccggatatg tttcccctga taggccttca tactcaaaaa attttgcttt
    22681 cgttggcata atgaacctta aacccaatgc gggtaattcc ggtaatcctt tttgtccata
    22741 ataatgcata ttgacaagca ttttaccctc tccagcgatt tggtaggaaa ttgtcacttg
    22801 ggtttttggt actgtgttga tttccagtgt aaaaatgatt tcaaccgttt cagcgtattc
    22861 attacttgaa tattgattat ttactggagc aatcggcaaa tcgatctttt tattgtccac
    22921 cagtacttct atttctttga cttttggata caatccggct cctaaccaca taccggattt
    22981 aaatccaaat ccgctacctc tgtcattatc ggtcgttgct cgccaaaaaa ctggaagtgt
    23041 ttcccggtat agccattctt ttccgttttt attgagcgat tccagtccgc ctcttgtata
    23101 agaaaaaata taatgcgtat tccccgattt gacacctaat gtacagtcgc cgaagacgat
    23161 tgcaactttt tccgtatcca tatgactttt atttactgta tttttcataa taatatctta
    23221 cctcctgcat ggcgggtttt tcttgtccat ctgcaaaaac gatcccattc cctgagaatt
    23281 catagtcact tggtttatcg tcaaagtcac cgccatagcg catcacttct ctgcctgtga
    23341 ttggatcagt cacttggatc gcctgatctt taaaatccca tatatagcct ccttggtaca
    23401 tttcataacg atcaagcaac gtcatgtaag aatccattcc acctaacgaa ttccccatgt
    23461 catgcatata ttcacataag atgaacggtt tttccgcttt attttcaagg tagtcaatga
    23521 tctcttctgg tggcgcatac atgcagcttt ttacatctga aatcgaatct tcgaatgctg
    23581 gatttggaaa attcccttca taatggacaa gtcgatcggg gtctactttt ttgaagtaat
    23641 cgttcatttg tcgaatgttt tcttccgcat aagattcatt tccaagagac caaaaaagaa
    23701 tacttggatg gttttttaat gtttcgaaat tcgtttttgc tcgatcaaga acagccgcct
    23761 gccactgagg tactgcacct ggtacgttgt aagaaggctc aaatgcgccc attttttgcc
    23821 atgagccatg ggattcgagg ttcgtttccg ccatcacatg gattcctgct tggtcgcata
    23881 aataatacca agtgatctgg ttggggtaat gagatgtccg tacgccgttg atctttgcat
    23941 tttttatgat agccaaatct ttttccatat cttctatagt gatggcgcgt ccttttttcg
    24001 gatgccactc atgtcgattt accccgttga taatcatccg ttttccattg aagtagatga
    24061 ttttatcttt gatttcgatt ttacgaaaac cgaaggaata gtcaatatac tccgttactc
    24121 ttttttcttc gtctatcgtc tccaattcaa gcttatataa attgggctga aaatgccccc
    24181 acagtgccac attttcaaat ccatcgatgc gaatcgtcat ttgctctttt aacggatatt
    24241 gctgttgata gatttccttc ccatcaggat ctagtatcgc tacattcagc tgactgtttt
    24301 tggctacttg ctcaaaagac aaatctactt caagtttgcc attttgacat ttgtcatcta
    24361 agcgaggttt gatccaaaga tcttctaaat ggttttgtcc tcgagcaact aagaagacat
    24421 ctctgaagat cccaaaaaat cggaaaaagt cctgatcttc caagaatgca gctgtacttc
    24481 ttttgtaaac ctccacagct agaatatttt cttcttccaa ataaggtgtt aaatcaaatt
    24541 cagacggagt gaacgaatct tcggcatacc cgataaaatg accattcaac caaataaaca
    24601 ttgcttgttc tacaccagca aaatgaatcg agatttgttt tccctttaga ccttcttcta
    24661 atcgaaaagt cgtcaaatac gaaccaaccg gattgtcttt agcagctgaa aagatccctt
    24721 ccgcctcttt ctcagggctt gtcgtgaaag ggggacggcg aaatacatgt ccctcccacg
    24781 gatacatcgt attgatgtaa tggatcttgt cataatttgc taattcaata tgactaggaa
    24841 cagtgatttc atcaaaagaa caggcatcaa aatctttttt ataaaatgtt tttggtcgta
    24901 ccgaaggatt tggcgaataa caaaaacgcc atgatccatt taaagactgt ttaagggagg
    24961 atgtggtttc tacttcttcc cttgttgcat aacaaacatg atcgctatgt gcatttgatt
    25021 ggttcactct aaaaacttgt ggatcatcta accattttat atttgctttc atactttttt
    25081 ccttctctct cataactttt tgaaatgaag cagttctgtt tggaaatcac cttgaaaatc
    25141 ggggaagtaa agtccaactg tcatcaattc ggcccccgaa aatatccgct tcgaggtctg
    25201 ttcctgataa agtgcctcat catctaaccc catcagatat acttcatgga acgctggttg
    25261 agctgaagct agtatcctcc ctaaaaacac aatagcttca tcttgtttag gagacacaaa
    25321 catccatgcg gcaatatttt cttcaaaagg actcgccaaa cgataataat ggccatactg
    25381 gatcaccgga cgaatcgttt gataggatat aatttggtct tttacttgct tttcttcttc
    25441 aggagacagc tttgtcaaat ccagttcata accaaaaaca gcgctcatag ctgtttctcc
    25501 tcttgttttc aacgaagtag atctgtcggt ctgatgattt ggtacagctg atacatgagc
    25561 tgtgatggac gaaatcggat aagcaaggct tgttccatat tggattttca tacgctccac
    25621 tgcatccgtg ttatcacttg tccatgactg cggcatataa taaataaacc cggcatcaaa
    25681 ccgtccacct ccaccagaac agccttccca taagatttcc ggataatcag ttgtcaaatg
    25741 ttcaagtagt tcataaagcc cgagcatata acgatgcgct acttctcctt gtctctgtgg
    25801 gtcaagggta atcgaataga catctgaaag cgaacggttc atatcccatt tgatgtagtc
    25861 aagaggaatc gtatctaaaa tctttctcat ttgtttctca atcgtctgcc gaacatctaa
    25921 ccttgtaaaa tccagtacat gttgtgatct ggaagcagaa ggcattctac ctggctcctg
    25981 cataagaaaa tcaggatgtg ttcgatacag ttcagaatca accgagatca tttcaggttc
    26041 aaaccataaa ccaaatttca atcctttgct atgagcataa tctgctattt cacgtaatcc
    26101 atttgtcaac ttgccttcat actcgaacca gtctcctaat gaagaattgt ccgcatctct
    26161 ttttccaaac cagccatcat ctaatacgaa catttcgatg cctagctcct ttgcttcatc
    26221 aatgatcgcc ttgatttttt cactattgaa gtcaaagtaa gtggcttccc agttattgac
    26281 cagaatcggc cgttcagcaa attgatgacg acctcttgcg attctttcac gtagcaactc
    26341 atggtggatc tgactcattt tgttcaatcc ctgacttgag taagaaagga tcatttcagg
    26401 tgtttgaaaa gactggcctg cattcaattc ccacgaaaaa cgatggctgt tgatcccacc
    26461 gacgactctc aattgattga tctgatcttt ttcaagttcg aaagaatggt tgccggaata
    26521 aacaaattga agtccgatcg cttctccagt attttctgtc gtatgatgat gaactaaagc
    26581 aataaagtta ttcatatggt gactgctcgt tcctcgacgg ctttcaaata cttttgttcc
    26641 agataaaaca gattgccgtg aaatctgtct ttcattgaca tgtgctcctg gcaaagcaat
    26701 gacttcatca aatcgcctag tatgggcaaa gtctagctgg aaactagccg ctttttctag
    26761 tttgattgtt tctccggttt catttcttat ctttacagat cgtgtgatca ctgctctatc
    26821 agtaaatatg gtatagaaca aatcgaaata aatcgctgtt tctctatcct taagtgaaat
    26881 caccaacgtt tgtgcctctg acttgtccat cacataagtt tgaggcaagc cttcgatctc
    26941 tggcttccca tctgtaatat aatggctgtc atatctcaga tccagtaagt ctgatccatt
    27001 cttgcgctga atgatcgatg cgggcgttcg aaagtccatg ctgccatgag aagagtattc
    27061 ttgcggtagt gtatctttcg acagagaccg gtcaggattt aagggaacat ttcctgaaaa
    27121 tccacgatct cttcggggat aatttttatt gtctttgtac tgcttgactg gtttgccaaa
    27181 ataaatatgc gccaatacat tgctttcttc tatctttatc agataagaaa gaaatgtatt
    27241 tcgtaaatgg aagactcggc tcttttcatc aaataagatg aaatttttat ctgtttttct
    27301 atgcgtaata tccatcattt cactcccaac aaaacggaaa ccgtttactt ttttttataa
    27361 ttcctgtata ctgtttacta aacaattttg tcaataaaaa ttttttattt ttttgaggag
    27421 tgtgtaaaaa tggttggaat tagagatgtc gcacgaaagg caaatgtctc tcctgctaca
    27481 gtttcccgtg tgttgaataa cgatcagacg atccatgtgg ctcagactac tcggcaacgg
    27541 atagtagagg cagctgctgc gttggattat gacatcagta ctcgtaaata tatcaaaaag
    27601 agaatgcctt cgattggtgt gatctctacg atcggcaaag cgagcgaaga acaggatatc
    27661 tattacaaac atttgcgagt agggatggaa gaagaagctc gacgtctgca tttagggatg
    27721 aatcgaatct ataatctatc tgataatcct aaacaatggg aagatctaga tcagttaggt
    27781 gcggtgatcg ttgttgggac tgttacgaaa gaatcgatta ccgccttgtt aaaacaaaac
    27841 atgcaaatag tcgtcgtgga taaccctgac atccagcaag aggtcgatat ggtgtatgca
    27901 gatcttgaac ggatgactaa aaccgtactg gaattgttct tagaaaatgg tcataaaaga
    27961 atagcctata tcgggggata taaagtcgat atggatgaat tcggtgggaa gcagatttct
    28021 ataaatgaaa agaggatgcg tgcctatcag cattttatga tcgatcatca cttagaaaaa
    28081 tatatgtctt atcatctagg tggctggaca gaaaaacaag gtgaggtact tgcgactaat
    28141 ctattagaaa atgaatcccc tcttcctact gccattcttg tcgggagcga tccaatggca
    28201 attggtgtct atcgggcatt gaaacaaaaa aatatccaaa ttggagaaga gattgttgta
    28261 gcaagctttg ataatatcga gacaatcgca aacctctccc ctggcttgac cagtgttcaa
    28321 atcaatgcga aatcaattgg gcaagcagct gttcgaatgg cattggagcg aattgacggt
    28381 atacgggacg aaccaatcat tttgacttat cctgcacgat tgattgttag ggaaagtttt
    28441 atcccaagaa caaaaaggta ttgaaagaga ggttgtgaaa agccgcttcg tttcttcata
    28501 ctcaaaacga aacggctttt tcacaacctc ttacttatac ttattcacaa aatccaatgt
    28561 ctgctggtaa caattttgcg attcttttag cgtgtaatca aattggtatt catgggtaat
    28621 ctcttttttt gtatctgtat aaaacaagga ttgcacgggt acctttaatt ctgtcaaccg
    28681 attttctaat gcttgacctt gctcctggaa tgagtatgca tttccgtcag ttatatacgt
    28741 tggcggaaat tccttagaaa catggtcagc cacactagct tctttcaatt gctcactcgt
    28801 tttccaatct tttgttccaa tcaacgacca cgcaacggtt ttgacgaaaa acttcataaa
    28861 tcgatcatcc gaatgctgct gtgctgttag cttcaaatca acaggtccgc aataagaaat
    28921 ggttcctttc agactatctt ttggaagtag atgtttgatt ttcatttgtt tagcatacgc
    28981 atcattcgtt tgtatagctg catattgcaa tgcgatttgt gaacctgcac tatctccacc
    29041 aaaaaataat tgttccatgt ctagcaattt gtcgtctttt tcttcagcta ataaatcttt
    29101 gatcagttca ttcacttgaa gaacttgatt agggtattct gaatcaggcg ctaattcata
    29161 attcatcgca accactgcga tgttggcgtc tgaaactaat tttgtcgcaa attctttgac
    29221 acccgattta tctccgccga cgaatcctcc accatgtacc caaaatagaa caggaacagg
    29281 ccccttgctg ttttttggat agtagatgtc ataagtattc tctttatact tggactcata
    29341 cgtcaaatct gtttttttcg ccacgttttt ttctgcttga tcgaacgttt ttttatcagt
    29401 gatttgaaca gtatttgaaa acatatggtt gatcaagaat gctccaggtc tgggggaaag
    29461 ctggaaggca agaaagattg caaacgcgat cccaagaatg attccgaaga accatccgat
    29521 aaatttaaga attttctttt tcataaggct ccctcagtag ttagtattaa gcattctgat
    29581 tataccatag tgttatttcc tatcaagctc tcctagatga tttaatgatt ttccccaaac
    29641 ttcttctgca atgcttttga caaaagaaat tttttccatt gttcttcaaa caaaacattt
    29701 tcttcttctg ttgaagcaaa tccacattga ggacttaagc acaaacgatc caaaggcaga
    29761 tatcgtgtcg cttcgtagat ccggtcaatc acttgctggt gctcttccat ttcgccattt
    29821 ttcgttacag tcaaaatcta agacaactaa tttatcttca gagactttct ctaaaagaac
    29881 aaaactccca gcacgttcac tatcgtattc taaataatag gcacggacat tttcttgagt
    29941 aaaataatgg gtctgctacg tcgtcatccc ccctgatgcc gccaaaatcg aataataatt
    30001 tccgcgtcaa acatgtatat ttagtgttga atcatttgag aatatgaaaa taaaaatctt
    30061 tttgatcaat cagttaatca aattgaaata acgaaaaatc tgagacagaa tgacgttcat
    30121 tctgacccag atttgatttt gcacaactca acagtcctta ttggcaaatc gccaaaataa
    30181 tgtagactaa ctaaaaagac taagaaacaa taacaaaagt taacacttct tagtctttct
    30241 ttagcacgtc tatttattaa agattttagg gccttttctc atgtttttac gatcattctt
    30301 aggtgtttcc atttgtttgg atcggcctcc accttgtttc tttttgatca attctttcat
    30361 tttttctttt tggtccattt ttattctcct ttaacgaatg aagcgactaa ttcgatatga
    30421 tgggtttgcg ggaaaagatc tacaggctga actttcttca cggcataacc tgcttctgca
    30481 aataacttag catctcttgc aaaagttgcc ggattacatg agatatagac gattcgttct
    30541 ggtgacattt cgattgctga ttcaataaaa tgatgatcta atccttttcg tggagggtca
    30601 actacgatca cagatggttt gatccctttt tcttgccatt ttttcataac tttttctgct
    30661 tttccgactt cataatgaac attttcgatt ttattattta aagcattgaa ctgtgcatct
    30721 tcaattgctt ctggaataac ttccatccca tacaccttat ccactctgtc tgctaaagac
    30781 aagccaatcg tacctattcc tgaataagcg tcgatcacaa tgtctttttt atctaactcg
    30841 gcaaactcga tggctgtttg gtacagatac tctgcttgtt gagtatttac ttgataaaat
    30901 gactttgctg agattcgata ggtttttcct aatagctgat cttcgatata gtctctgcca
    30961 tataaaactt tttctttgtc tcctaaaatc acatttgttt tttcttcatt gatattttgg
    31021 atgaccgaaa ccacttctgg caattcttca tgaatagctt cagcaatttc cttggattta
    31081 aagaattttt ctttacgagt aactaaaacg accatcattt catgggaata atgaccacga
    31141 cggataatga tgtgacgaag ctgtccttca tttttttctt cattatatcc tcgtacttgg
    31201 aaccgttgca agatatctct gactactttg attgcttgat cgatgacagg atcttggata
    31261 taaaaatctt cgatagggac tagatcatgg ctattctttt tgtaaaaacc ggtggctaac
    31321 tgtccatcga tacggcgaac agggatctgt gctttattac ggtagccgaa tggttctgcc
    31381 attccgaccg ttggaagtac aggaacttct ggcatcttag caatcttcgc cattacatgc
    31441 tctacttgtt tttgtttgaa gacaagctgt tgttcatact tcaaatgggc taaaggtgcg
    31501 atacctatgc gaagtaatgc attgttttct acaggctgac gatcaggtga ttgtgtaata
    31561 aatgtttcga cttttccata tccaaactta ttgcctgctt ttactactag tatttctact
    31621 tcttctccag ggagtgcatt ttcgataaat aatggataac catcgatttt tgctaccccc
    31681 attccttcat aactcaagtc gacgatttcg actgtatatc gttcgttttt ctttactatt
    31741 tgctgttctg tcatattttg ctcctttaag cctatttttc aatcctactg aactctacta
    31801 taaatcagct aattacacaa ctaaaaccgg cactgacctt actacgttca gcaccggctt
    31861 ttgattaatt cttttgatta ggtgtttcgt ctgctatttt tccgtcaccg tcgatatctt
    31921 catccgttaa acgttcgact tcttttacga attctttgct gacaacctct aattcttctt
    31981 catctgtacc tgtgattgct tcatcaggaa tctgatcggt attggcaaag aactcaatgt
    32041 gttgatgaag attttggaaa tgcatcggtg cttccccgcc gtattcacca tctaagttga
    32101 tcattactgg tcgattgata tcatcttctg tttcaacgaa caaactgctt gttttggtat
    32161 agattattct tggatcatcg acatgtcgac cgccattcag catgagtgct gcaatatgca
    32221 agatctcaaa gatatttgct gtcttcacaa tgattaaaga aaacttccca tcatccaatt
    32281 tggcatctgg cgcgatctgt tcgaatcctc caacagagtt cgtcagccct aggaaaaaca
    32341 tcgaagcatt tccgatatat tccccttcat catacttcaa gcgcattttg attggcttta
    32401 ctcgagggag catctctgct cctttcgcaa gataagcaag ataaccaaaa atactcttta
    32461 gttctgatgg aacttcataa gtcaattctg tcaaatagcc acctgcagcg atattgatga
    32521 agtaactgtt atcagaaagg ccgatgtcca tttttacggt ttggtgtttt tttatcactt
    32581 ctgctgctgc tttaatatta tctctaggaa tttttaatgc tcgagcatag tcgttcgttg
    32641 taccagctgg gatgatggcc attttaggac ggtgttcaag cggagcgatt ccattaacca
    32701 cttcgttgat tgtgccatcg ccaccagctg ccactactag atcaaaacct agttctgcga
    32761 ctctacatgc ttcattcttg gctgaatctg gttctggtgt agttgcatag gcgctggctt
    32821 caaatcctgc ttcttctaat gcctgtagga tatccgccaa attttttttc aataattctt
    32881 ttcccgaggt ggggttataa attacgcgtg cttttttcat tttcagcctc tatcgtttcg
    32941 ctaattcatc tttaagtagc ttattcacga caccaggatt tgcttgtcct ttagttgctt
    33001 tcatgatttg accgactagg aagccaaccg cacgatcttt tccatttttg aaatcatcga
    33061 cagattgttg gttgttatcc aaaatttcat taatgattgg cagtagtttg ttcggatcag
    33121 ataattgcac ccaaccattt ttttcaacta cttcttgggc ttctccgcca tttgtgatca
    33181 attcacggaa gacttttttg gcgatttttg agctgatcgt tccatcggcg atcaaacgaa
    33241 tcattcctgc taagttctca ggtgtcaatt ttgtctcagc taattccagt ttctcactgt
    33301 tcaagtaagc agaaacttca cccatcaacc agttagatgc ttgcttggca tctgcaccat
    33361 tttccaaaac agcttcaaaa aaatcagaca tttcttttgt aagtgtcaat accattgcgt
    33421 catactctgg aagtccaaac tcacggatat agcgttcgcg acgagaagct ggcatctcag
    33481 gcaaactagc ttttactttt tgaatccatt cgtcatcgat cacgaatttc gggatatcag
    33541 gctctgggaa gtaacgataa tcactagacc cttctttgac acgcatcaaa actgttttat
    33601 tcgttgtttc gtcaaaacgg cgtgtttctt gttggatttc tcctcctgaa agaagaactt
    33661 ttgcttgacg tttttcttca aaagctaaac cttttttcac gaaagataaa gagttcaagt
    33721 ttttcaattc tgctttcgta ccgaactctt cttgtccata agggcgaaga gaaatattgg
    33781 catcacagcg catagagcct tcttccattt tcacatcgct gacttctgta aattggataa
    33841 ttgatcgcaa tgcttctaga taagcgtatg cttcttctgg tgaacgcata tcagcttcag
    33901 aaacgatttc gatcaaaggc gtgccttgtc ggttcaaatc aacgtaagaa taaccgccaa
    33961 taccatgcat gttttttcca gcatcttctt ctaaatggac acgttcgatc cggatttttt
    34021 tctttttacc ttctacttcg atttcgatcc atccgtcatg accgattggt tgatcaaatt
    34081 gggaaatttg atatgctttt ggattatctg ggtaaaagta atttttccgg tcaaaatgag
    34141 tatctttaga gatttcacag tttaatgcca aagctgcttt catcccgaat tccagcgcag
    34201 ctttattcat tactggcagc acgcctggat aaccccagtc gatcacgttt gtattgctgt
    34261 ttggttctgc accaaaatga gctggcgctg gagagaaaat ttttgagttg gtttttaact
    34321 cgacgtggac ttctagtcca atgactgttt caaaattcat tcgtctttcc ctcctaaaat
    34381 agctggttgt tgtttatgga attctgttgc ttgttcaaac gcatatgccg cttgatacat
    34441 cgttttttca tcaaaataat taccgatgat ctgcaatcca actggtaatc cttctgataa
    34501 acctgccggt acagacatac ctggaagtcc agccaagttg acagggatcg ttaagatgtc
    34561 actcatgtac attgtgattg gatcgttaat gttctcaccg attccgaatg caactgttgg
    34621 tgaagatggc ccgatgatca agtcataatc tgcaaagact ttttcaaaat ctcgtttgat
    34681 caatgtacgg acttgtcctg cttttttgaa gtaagcgtca taataacccg cgcttaagga
    34741 gaatgtacct aacatgatac ggcgttttac ttcttcaccg aatccttctg aacgtgagtt
    34801 gacatataca tcatctaatg atttcacatt ttcagagcga taaccgtagc ggataccatc
    34861 gaatcgttgc aaattggaac ttgcttctga tgaggcaatg atataataaa cagccacacc
    34921 gtattttgaa tgaggcaagc tgacttcttc cacaacagca cctaatgaac ggaatgtttc
    34981 tgctgctttc aatacagctt cttttacgcc aggggccaca ccttcgccta agtattcttt
    35041 aggtaaagca atcttcattc ctttgatgtc gcctgtcagt ccctctgcaa aatcaggaac
    35101 tgaaacgcct gaagaagtac cgtctttttc gtcataaccg ctgatagctg tcaatgctaa
    35161 tgcattatct ttcaccgtac gggtcattgg tccgatttgg tcaagagatg aagcaaaggc
    35221 aatcaaacca aaacgtgaga cacggccata agtaggtttc atccctacga tgccgttaaa
    35281 agctgctggt tggcggatac ttcccccagt atcactacct aaagaaactg gaatttgtcc
    35341 agctgcaact gctgctgcag aaccaccaga agagccacca ggtacttttg ttgggtccca
    35401 agcatttttt gttttcttga aataagaagt ttctgtactt ccacccatag caaattcatc
    35461 catgtttagc ttaccaacag ggatcatatc tgcttgatag attttatcca tgaccgttgc
    35521 atcataaatt ggttcaaaat tgtaaaggat cttagatgca gcggttgtca agatatcttt
    35581 tgttacgata ttatccttga taccaattgg aataccagca aggacgttgc tttcatcaat
    35641 ccctttttca tctaaagctt ttgcctgttc caatgctttt tcttcgttca atgtaatgaa
    35701 tgcctcgacc tcaggctctg tttctttgat tcgttcaaat gttgtttttg tcaaatcttg
    35761 tacagtgatt tctttcgata caagcatatg atgcagttct tctagagaac gattgtgtaa
    35821 ttctgtcatt atgcgccagc ctccccatta tcgatgattg cgggtacttt gataaagccg
    35881 tcttctgttt ccggcacatt tttcaataat tcatcgcggt tccatccagc ttgcgcccga
    35941 tcttgtcgca tcacgtttac tgtttcaacc acattcgacg taaatggaac accttccgta
    36001 tctacttctt ccagcatttc aaccatatcg atgattttac ctaattggtc ggtaaaactt
    36061 gcaagttctt catctgcgaa cgcaagtttt gctaatttgg caacgtgttt cacttgttct
    36121 tcactaatcg ccatactacc ccttctttct aaacgagtta aaaactttgc tctctatttt
    36181 aacacaagag tgccgtgtct gaaattgttt attgaaattt gtctgaaaaa actcattttt
    36241 cattcttcta ctgagcgact tttagccaat gcatctgcca tttgatcttc tgtccaaact
    36301 tcgattccta attcttgtgc tttggtcagc ttgctgccag catcttctcc tgctaccacg
    36361 atatccgttt ttttcgatac gctaccagtc actttcccgc caagattttc gatcgtctct
    36421 tttgcttctt cacgagtaaa tcgtgttagt ttaccagtaa ggactactgt tttttcttta
    36481 aaaggagact cgacttcttg caattgggca tttcgaagcc ctttgtattc gaaattgacg
    36541 cctgcttgtt ttaattcatt catcaattca tggacttctt cgttactgaa ataagtgact
    36601 acactgtcgg caatgatatc accgatcgta tcaagcgcaa taatcgactc atagtcactt
    36661 ttactcaatg tttctagatc accgaaatgt tctgctaaaa tttttgctgc tttcgctccg
    36721 acatgtcgga ttcctaaccc gaaaatcaac cgctcgaccg aattatcttt gctgttgtcg
    36781 attgctgtca aaatattatt tgctgatttt tcttttattt tgtccaacgt caataactct
    36841 tcttctgtca atttgtaaag atcagcaaca tctgctacta gttttttatc atacatctgt
    36901 tctagtacgc gcggccccaa cccatcgata ttcatcgcat ttcttgaaac aaagtggttc
    36961 aaaccttctt tcatttgagc agggcattta ggattgatgc accgtaaggc tacttcttca
    37021 tccaaatgga caagttcact tccacaaact ggacaatgtg taggcaattg gtattcttcg
    37081 ctgtctttag gtcgtttatc taacacgact tgcgaaactt ctgggatgat gtctcccgct
    37141 ttataaatca gaactgtatc tttcaagcgg atatccttca acttaatata atcaccgtta
    37201 tgcaggctgg cacggcttac tgtcgttcct gctactcgga ctggtgtcat gatagctgtt
    37261 ggcgttacta cccctgttcg accgactgtc cattcgatgt tttcgattag cgtttcaact
    37321 tcttctggcg gaaacttgta agctgctgcc catctcggtg ctttgatagt aaaaccaagt
    37381 tgatcttgta gcgagaattc attcactttg atgacgattc catcgatttc atagggcaaa
    37441 tccacacgtt tgtcatgata ttcttcaatg tatgaccaca cttcatcaat cgaatgacat
    37501 agacgttttt cccgattcgt gtggaatcca attttttcta gctcttctag cgcatcatac
    37561 tgggtttttg cttgcatagg accgaaatca gctactgtgt ataaaaaagt gtccagattt
    37621 cttttcgctg tgattttaga atctaactga cgcaaagaac cagctgcggc gttccgagga
    37681 ttcgcaaaga tatcttttcc ctctgcttct ctgtcttggt tcaattggac aaatgaccgt
    37741 tttggcatga aacattcgcc tcttacttcg atgttcattg gttcttttaa tttgattggg
    37801 accgaccgga cagtttttag gttttcagtg atgttttctc ctacagttcc atccccgcga
    37861 gtcgcacctc tcacgaaatt tccatcttca taacgcaaag aaacggaaag tccatctatt
    37921 tttagttcac agcaatagtc aactacacgt cccactgctt tttgtacacg ttgatcaaaa
    37981 gctatcagct cttctttgct gaaaacgtcg ttcaaactgt aaagcggaat atcatgagtg
    38041 actttttcaa aaccttctaa tacttttccg cctactcgtt gagtgggaga atcagacgtg
    38101 atcaaatcag gatattgttc ctcgattgct actaattctg cgtattcttt atcataaaca
    38161 tagtcttcca ctgttggttt atcttctacg tagtattcgc gagaccattg gttcagtctt
    38221 gtacgaagtt cttctgctcg tttagcagct tcgtcaatcg tcatttcatg ttccatcatt
    38281 taccctccac tactaagctt tttcaattgg cgcaaaggca gccaaaagac gttttactcc
    38341 ttgacttggg aaagccacat ctagttctag atccttcgac gtcccgctta ctcggacgac
    38401 tgttcctaga ccccattttt tgtgtttgac tttatctcca ggtttccagc tgttattttc
    38461 ccctccagta gcacttttag agggaacaac cgctgtctct ggctgtttat agctatatgc
    38521 tgttttttta gcgaatacag gcgttttttt aggcgtagga cgcatgcctt cgatttccag
    38581 taactcctga tcgatttctt ctacaaaacg agaaggtcgg ttatattgcg tccgtccata
    38641 taatgttctg gagaaagcat ttgttaaata caacgcttct tctgctcgcg tgatccccac
    38701 atatgcgaga cggcgttctt cctccagttc gctttcttcc atcaatgctc gagataaagg
    38761 aaaaaccccc tcttctagac caatcaggaa tacaactgga aattctaatc ctttggctgc
    38821 atgcaaagtc atcaatgtca cttgggaagc atcttcttct aaattgtcaa tgtcggaaac
    38881 aagtgccaga tcatttaaga agaccgttag tttttcttct ggtgcatcag cgtcttcttc
    38941 attttgctgt tcgaattgtt tgtcgaattc ttgtgtgacc gttaaaaatt cctccaagtt
    39001 ttcgatgcgt gcttgtgctt ctaatgtatt ttggattttt aagtcttcta gatatccgct
    39061 acgatccaaa acctctttcg tcaattctgt cactgtcaag tatgggatca tctgagtgac
    39121 ttcttggatc atctctccaa atgcacctaa ttgctgtcct gcttttccac cgatatttgc
    39181 cagatccaca ttttgcgctg cttcaagcag tggccattca tgaagagaag caaaactgcg
    39241 tagtttttcg atagatcctg gtccgatgcc tcgtttgggc gaattaacga tacgctcaaa
    39301 actgattgaa tcttgaggat ttaccagcac attcaaatac gctaaaatat ctttgatttc
    39361 tttacgatcg tagaacttgt gccctccaac cattttataa ggaatatttg ctttcaacag
    39421 agtttcttcc attacacgag attgtgcgtt cgtccggtac aagatggcaa agtcgccgta
    39481 attccgatga ttgcttcgaa tctcttcttg catccggtct acgataaaac gggtttcgtc
    39541 tcgttcatta tctgcacggt agtaagtgat cttgtttcct tctttgtttt ctgtccacaa
    39601 gttttttggt tttcgattgc tattgttttc aatgacttgg tttgctgcgc taaggatgtt
    39661 tttcgtggag cgatagtttt gttctagcaa aattactgca gcatctggat aatctttttc
    39721 aaaatccaag atgttctgca tatctgcacc gcgccagccg taaatacttt gatctgcatc
    39781 tcccacgacg cacaggttcc taaatctacc tgccaataaa ttgactagcg tatactgtgc
    39841 gtgatttgta tcttggtact catctacatg aatataatgg aatttgtttt gataatacgt
    39901 gagtgaatca ggatgttctt caaataatcg aatcgtattc atgatcaggt cgtcaaagtc
    39961 catacattga ttatattgaa gctctttttg gtaagcagca taacattttg ccgcaatctc
    40021 ttcgtaataa ctgccttgca tttttgcaaa atcctgaggg gtctgcaggc tgttttttgc
    40081 ttgagagatt gttcctaata tacttcgagg atcatatttt ttcggatcaa tgttcagttc
    40141 ttttagtatc cgtttcatca aggtcagctg ttcagaagaa tcgatgattg taaagttccg
    40201 gttatacccg ataaaatcaa catctcttct taaaatacgc acacacatcg agtgaaaagt
    40261 cgatacccag acatcttcgc ctccagatgc gagaatcgca ttgacacgtt ctttcatttc
    40321 ttttgcagcc ttattggtaa aagtaatcgc taggatgttc caaggattga cttctttttc
    40381 ctcaatcaaa taggcaatgc ggtgtgtcag cactctcgtc tttccacttc ctgcacctgc
    40441 cattagcagt aatggtccgt cagtgtgaag aacagcttct ttctgacggg ggttcatccc
    40501 gtttaataat tcagctttgg atgacattaa caacatcctt tcttttatca atcttgttaa
    40561 ttatagcatt ttcttggctt tcattaaact agctagttca aaatcatttg tttcttttta
    40621 tggatttttc cttgtcaatt cgttgatttc gcgctaaatt aaaagaaagc gaggcgtttg
    40681 aatggaaaaa aaaggaaaaa tcaagaaatt agataagact cagatatatt ttattctaaa
    40741 aggaatattt gttggactgc tgacaggaat cgtcgttagc ctgtttcgat tatctatcga
    40801 aaagctaagc gatttagttc gaagtatcta tgaaatgagt cgggaacagc cgatttactt
    40861 aattggccta gctatttgct gcatccttgc agcatttttc gtgggttatc tggtcaaaaa
    40921 tgaaccggat atcaagggaa gcgggattcc acaagtagtg ggacagcttc gcggggaatt
    40981 atcaatgaac tggttctctg ttttatggaa aaagttcatt ggcggtgtac tttcagttgg
    41041 tgcgggcctt tttctaggta gagaaggacc ttctatacag cttggcgcat ccattggaca
    41101 aggagctgga caattgtttc ggagtccatc ttcagaagaa aagattttaa tttctagcgg
    41161 tgcaagtagc ggacttgcag cagcattcaa tgctcctatc gcaggattac tctttgtttt
    41221 agaagaaatc catcactcct tttctccact tgtttggctg acatcatttg cttcagcaat
    41281 cactgctaac ttcgtctctc tttacttttt cggcttgaga cctgttttat atctaggtga
    41341 ccttccttct ttgccgttga aatattatgg ttccttagtg atcttaggag tacttctagg
    41401 catcttaggt tttatttatc agaatgtctt gctggctttg ccaaaatttt acaaccgatt
    41461 gcctttacca gcttattttc acgggttcgt tcctttcttg ctgattttgc caattggtta
    41521 tttatggcct agtactctag gcggaggcaa tcaaatcgtt ttagcttttg gacatgcttc
    41581 cttgccaatc attatgatta tcggtttgtt tgttcttcga ttcgtttttt ccatgatttc
    41641 ttacggtggg ggtcttccag gggggatttt ccttccgata ttgacattgg gggctttaat
    41701 cggtagcttt tatggaaaca ttatggttga tttcgggatg gatccaatct acatcaagga
    41761 ttttgtcgtt attgccatgg ctggttattt cacagcgatt ggaaaagcac cgctgacagc
    41821 aattatttta gtgacagaaa tggttggctc tcttaatcat ctgatgcctt taggattggc
    41881 tgctcttgtt gcttatatcg tcaatgacct tctcggcggt aatccaattt acgaatcatt
    41941 attggaacgt ctacttggaa atactcagac cgcctcacta aaaggtgcaa ggacaacttt
    42001 tgattttcca gtaacggcag agagttcttt agacggatta atggtacgag acttcaattg
    42061 gcctaaaaat atgctgctaa tctctatacg cagaggaaat caggaaatct tgactcacgg
    42121 tgatacgatc atgaatgtag gagatatttt ggttatcttg actgatgaag caaacttgcc
    42181 aagaatcaag caggaaatcg aggctaaatc ttctatcaca aatttgaagc aatcatttta
    42241 acgaacatgg aaaaactatt ttacaacaac tattcatcta cactacttga ttctatatga
    42301 ttctataaaa aaagaagagg ctgggaaaac ccagcctctt cttttttatt tctatgcttc
    42361 tgctactgca ggagcttttc tcaataaacc ataaatcaca ccagatacga ctgctccgat
    42421 agcaataaac accaaataca tgaccgggtg actcagtaag aatacaacga aaatcccgcc
    42481 gtgaggagcc agtaatttga ttccgaaagc tccgactaat ccgccagtta aagcagaacc
    42541 aataataaaa cttggaatgg cacgaatcgg gtcagctgct gcaaatggaa tcgatccttc
    42601 cgtgacaaac gatagtccca tcacgatgtt cgtcaaacca gcatcttgat ctgttttgct
    42661 gaatttattc ttaaacaaac gtgtagcaac gaaaattgcc aatggaggta ccattcctcc
    42721 agccataaca gaagccatca cgacacttcc gccagttgca acagtcgtag ctaaagttgc
    42781 tgtaccaaac acataagcgg ctttgttgat tggaccgcct aaatctgccg ccatcattcc
    42841 agctaataat gcaccaagta aagcagcatt cgaaccactc atacttgtca agaaaccgtt
    42901 caatgcatca ttgattgctt tcatagggac attgaccagc aacatcaaga aaccagtaat
    42961 cagtaagcca aacactggat aaaagaggat cgttttaatt ccatccaatg attttggtaa
    43021 gttcttgaat aatttacgta aaaatacgat gacataacca gctaagaatc caccaatcag
    43081 tgcacctaag aatcctgcac cgcctgtatt agctaatgca ccagcagcaa atccaacgat
    43141 caaaccaggg cggtcaccga tacttgaagc aataaatcct gctaataccg gcagcatgaa
    43201 accaaatgca gcttgtccga tttggttgaa ccagctagca gcttgattgt aattccctag
    43261 atttgataat tgatcatgtg gcacgccaat aaattggtcg atcatgaaag aaagcgcaat
    43321 agcgattcct ccaccaataa cgaaaggtaa catgtgtgat acgccgttca tcaaatcttt
    43381 gtaaattctt tgtccgatag taccatccgc atttccttct tctgaactgc tttcttttcc
    43441 atcaccatgg aatactggtg cagtaccact aagggctaga ttgatcaact cttctggttt
    43501 acggatacca tcactgacag gacgattgat cagttctttt ccgtcaaagc gattcatctc
    43561 tacttttttg tctgctgcaa cgattacacc ttctgcacgg gcgatgtctt cagcagtcaa
    43621 tcggtttttg attccttctg aaccgttcgt ttcgactttg atctccacac ccatttcttt
    43681 cgcttttttc ttcaaagcat cttctgccat gtaagtgtga gcgatccctg ttggacaagc
    43741 tgtaacggca acaacgaatt ttctttctgg atgagcagaa gcttctgcag cttgtgtttc
    43801 ttgttctgct ttttcttctg cttctttttg ctcttcagct gtttggaaca agtcttggac
    43861 ttcttctggt gtatttgctt cttttaattt tcctacgaaa tctgcatcaa tcagcaatcg
    43921 agatagtgct gctaatgctt gcaaatgtgt gtcgtttgcg ccttctggag ctgcgatcat
    43981 gaaaaacaga taagttggct gtccgtctaa ggcttcataa tctactcctt tttggctttt
    44041 tgcgaataaa actgttgctt ctttaactgc actgtttttc gcgtgaggca tggcgattcc
    44101 atcaccaaga cctgtagaag tttgtgcttc gcgggctaga attccttctt tatatgtttc
    44161 gatatcggaa atccggccgg catcaaacat tttttggacc atttcatcga ttgcgccttt
    44221 tttatcagtc gcttgcaaat ccatgatcat cgcttctttg accattaaat ccttgatatt
    44281 catgattatc ctccttgaaa ctaatcaatt tttgtgattt caacttctgg cagcaactca
    44341 tcaatcaatt cgcgagctgc caagtcatca gaaaaagctg ttgcgcttcc gcaagcaact
    44401 ccccatttaa aggcttcgat tggatctttc gtacggctgt aagccccaat aaagccagca
    44461 atcattgaat cccctgcacc tactgaattt ttcaatgggc gaactaatac attcgtttta
    44521 taagttcctt gttccgtgaa gagcaaagca ccgtctccag ccattgagat cagcacattc
    44581 tgtgcccctt cagctagtaa ttttttccca tacggaatga tttcttctac agattggaag
    44641 gttgtgttgt aaagctcagc taattcgtga ttgtttggtt tgatcaacaa cggtttctta
    44701 acaagtgcat tttttaggtc atcacctgtt gtatcaatca cgaaatctgc acctttttct
    44761 cgaatgatat caatcagttc ttgataaaat ccttttcgca ggctagctgg cgcgcttcct
    44821 gacaatacga cgatatctcc ttgtcctact gttcctaatg cttgcttcaa ctcatcgatt
    44881 tcttcatctg aaatagatgg gcctaagcca ttgatctcag tttcagtgtc tgattttagt
    44941 ttgatattga ttcttgtatc ttcttttacg gttgtgaaca aagactggat ttgttctttt
    45001 tcaagccact tcgaaataaa ttcgccagtg aaccctccta aaaaccctag tgctgtagat
    45061 tgagaatcga tccgtttcaa aatacgggaa acattgatcc cttttcctcc aggtaacttg
    45121 aagtcttcct tcattcggtt caaatcgcct agttttaacc catctacgcg tacgatataa
    45181 tcaattgatg gattaagtgt tactgtgtaa atcattgtat tgcctcctta attgttgtct
    45241 tttcacggaa ttcattaacg aattctaatg gacaacgctc ggttatgatc gtggctgctt
    45301 ccaaaggtgc aacagaggtg aaggacaact ggttgaattt ggtatgatca atcaatacaa
    45361 atgtctcttc ggaatttttc atggctaaac gtttaacagc tgcctcttct ggatccggag
    45421 tcgtgaatcc ttgttcaaga tgggcaccgt tcattcccat aaatgcttta ttaaaatgaa
    45481 aattttttag ttgttctatc gcattagatc ctaaaacagc attagttgcc agctttatcg
    45541 ttccacctaa aataatggta gcaagctcca aatcaactaa atttgccgca tgttgaacgg
    45601 aatttgtcac tactgtgatc tgttttcctt gcagaaaagg aatcatttct aacgtagtag
    45661 agccagcgtc taaataaatg acatcttcgt cattaatggt ttcagctgcc gatttggcga
    45721 tttgctgttt ttcgtgaacg tttttgagtg atttttcact catattctgc tcaaaattca
    45781 ataattgctc tttcttagct ccgccatgaa ctctttctag aaacccacta ttctccagct
    45841 cttgtaaatc acgtcttatc gttgattcag aagcattcaa aaggtttaca agatcttgtg
    45901 atttcacgat tttctgctgt tcaagcaagt ctaggatttt ttgtctgcgt tcctctgtaa
    45961 gcattttcac gatcacctca atacaaataa tagcatattt tttttctact agcaatataa
    46021 atcagtcaaa aacgttcata aaaattataa atcagtcaaa aaaaaataaa aaaccagtca
    46081 gaaagactgg ttttttattt ttttgtctat catcaagccg ttttatcgtt ttttattttc
    46141 gcttcttgtt tttttcatat aaacagaagc aggttttctt tccttatgaa gaagataata
    46201 ataaagtccc atcccgccaa acatgaccaa aaaaccgatg actgatcgaa taggaaaatt
    46261 aggaacaaaa aagagacaga cggataataa tgatgccgga accattattc gataatagag
    46321 ttgtttatcc attttcttca cctcatattt tttgtaaagt aatcaattgt tctaattctc
    46381 ggtaatctaa aaattgcaca cgcagatgct gctcaccttt cttgacatcg aattggatac
    46441 ttcgccgctt tttctttatt cgtttgaagg taacatcaga gagtctatgt ttgatttcct
    46501 ttaacggtaa ttctgacagg gattctgaag gaacatagat tcttaaggat aaaagatact
    46561 cttgatattt ttcatctgtt acctctactt ctagatattc cctaatataa taacaataag
    46621 cttcgattcc attatccaaa gcaacagtcg gatcgataac tttatagcac atcatgcgtg
    46681 atcgattctc gtcaacccaa ggaaaatacg cctgagcatt tagtgttccg cgaacacgcc
    46741 tttctttata atctccttgt tggatatcat aactttttgt tacaaaaaat gaagcattct
    46801 ttttaggaac aggttgcatt tgtgcattct gatactggac agattggatc gtatggtcag
    46861 tgtaaatgta ttcaacgaat ggcggtttca ataaaccgac accattgtcc caagcaagat
    46921 gatcaaaccc aagctggtcc ctaaaatagg attgaaacat ttgattgttt ggcttttgcc
    46981 agttaaaggg gtctcgataa agctggatac atatgacttc gttttctaat tccttcactt
    47041 tttctacttg ttggatgtcg agaaaaatcg gtttagggat gatttgatga taatagcgac
    47101 tataatacat ttcccaacac gaagtgaaac ataaaccttg gtaaaaaaga tcgtatcctg
    47161 gaaattggtt gcaatcgact actacttccc cgttttgttg tttcatttta ggtaaatgtt
    47221 cgatttcatc ctttttctca aacaatagcc gttgagagat ctgttttgta ttgtgataga
    47281 taaactcgct ataagctcgc atataggcga acaccccatg agcaatcatt tttttctgaa
    47341 tgaagtctcg aatcaaaaat tgcttttctt tgaaaacaga gatagacaat acatttttta
    47401 tcaataattg attatcttcc aagcgaaaac tacattcgtt ctcgtcattt cctacaacta
    47461 accaactttc gtctcttacc gatgctagca ttattttttc ttgaagcttt cgaagatcat
    47521 acggatccgc ttgttgtgtg aaagagcgga taaatgttgg ctggatcaac agacgtgtca
    47581 aaatagccca atcgcttatt tcctgttttt tttcttcttc atttaagtct gtgagctgga
    47641 tagtcatcaa taatttatct tcgccaatca ttttctcacc tctttcaaat ctttttataa
    47701 tttaattata accaatgata aagaaaaact aaaactttat gtcttagaaa ccaatttgga
    47761 cgattacatg atagactaag taaaaagaaa caaagaggtg tttacaatgg ggttgaaatt
    47821 tgaacgtaca gataatttag ataaactttt cgaatcattt gctgtcgatc cggaaaaaac
    47881 agttgcacca aaagaagaga tcgatcgatt tctaaaacca cagccacaaa atgaaaagaa
    47941 aaaagacaag aaacaaaagt aagttaagct caaaaaaaga cttaccagcc aaacttttat
    48001 gaaagagttt gactggtaag tcttttttat tctttttatg attcaattgc ttgttcgatt
    48061 gctgcccacg cttcttcttt tccctgtttt gtaacggaag aaaagagaat aaacgtgtca
    48121 ttgcgatcaa aatccaacgc ttttttgatt tcgctctcat gcttgttcca tttaccacga
    48181 gggatcttat ctgccttagt ggcaacaaca attacaggga tatcataata cttcaaaaat
    48241 tgatacatct ggatatcttc ttgagaaggc ttatgacgca aatctactaa agaaacgacc
    48301 gcacgtagct ggtctctagt cgttaaatat gtctcgatca tctttcccca ttttgctcgc
    48361 tcagttttgg atacttttgc atacccatat cccggaacat cgacaaaatg taatgcgttc
    48421 tcgatcaaat aaaaattcaa tgtctgggtc tttcctggct taccggaagt acgggctaaa
    48481 ttctttcgat caatcaatgt gttgataaaa gaagatttcc ctacatttga tcgtcctgcc
    48541 agagctattt ctggcaattg tgtatctgga tattgttttg gtgccactgc acttatgata
    48601 atttcagctt gatggacttt catggtctct ctccttaaca aggtgatgaa taaagcaagc
    48661 ggctgtcgca aaagccaaaa tgacttttac ggcagcctct tttcatcaac ccgctttttt
    48721 ctctgaatct ttatagatta ctgttggctc accagacaat tcagctgctt cttttgtgat
    48781 aatcactttt tcgatgtttt catttgaagg gatatcaaac atcacatcca tcatgatttc
    48841 ttcaatgatg gaacgaagtc ctcgagcacc ggtattgcgt tcgattgctt ttttcgcaat
    48901 ggctctcaac gcttcaggtt cgaattcaag ttcggtatta tctagagaaa gaagtttttg
    48961 atattgtttg actagcgcat ttttcggctc agtcaagatc cgtaccagat catctgttgt
    49021 caatttctct aaagctgcca taacaggtaa tcgtccgata aactcaggga tcaatccgaa
    49081 ttttagtaaa tcttcaggaa tgatatgctg catgacactt tcgtcttcag aaagtttttg
    49141 gttttgtgta ccaaatccaa tcgtcttttc gcccatacga ttcttcacaa tcgtttcgat
    49201 gccatcaaat gcaccaccta cgatgaacaa tatgttcgtc gtatcaatct gaataaattc
    49261 ttgatgtgga tgtttacgtc cgccctgagg tggcacactt gcaacagttc cttctagaat
    49321 tttcagcaag gcttgttgga caccttcgcc ggaaacgtca cgagtaatag atacattttc
    49381 gcttttacgc gcaattttat cgatctcatc aatatagatg atcccttttt ctgcaagttc
    49441 cacattgtaa tcagcagatt gaagcaattt aagcaaaatg ttttctacat cttcccccac
    49501 ataaccagct tctgtcaaac ttgtcgcatc tgcgattgca aaaggtacat tcaatgtttt
    49561 tgctaatgtt tgtgctaaaa atgttttacc agaccctgta ggtccgatca agcagatatt
    49621 gcttttttgc agttcaacat catctgtgtt ttcttgtgct tgctgattga cacgtttata
    49681 gtgattgtat acggcaacag ctaacgagcg ttttgcgcga tcttgtccaa tcacgtagtt
    49741 attcaatact tcaagaattt ctagtggttt agggacttca gtgaattcgc gtacagcttc
    49801 ttcataaaat tcttcatcaa tgatttcttt acataggtcg atacattcat tacagatgta
    49861 gacccctgga ccagctacga tctttttcac ttcttcttgg gttttcccgc aaaaagaaca
    49921 gcgtacggtc tcattgctac ttggattgtc atacatggtc ttcacccctt aacttgcaaa
    49981 aagtatgcaa gaaactatca aagactttac agtcttttac tatcataaca tagtttagta
    50041 aaaagaaaaa gtgtttaaca aaggatcaac tgacctatac ctttgcattt tttactctta
    50101 acaaaaaata aggaactgtg acaaaatcgt gtcacagtcc cttatttcga ataaacaatt
    50161 ttctagaagt agcttcttca gaaatgattt attacttgat ttctattctt tcgcttctgt
    50221 cgctgtacct gtgatcaatt cgattgcttt tttcattgtc acatcatgtt ccagcatatc
    50281 ttctgtcaac acgcgtttga tttgttcgat tggcatgttg tatgtttctg ataattcttg
    50341 gatttctgca ttgatttctt cttctgttgc ttgaagattt tcagcagctg caattgcttc
    50401 gatcacaaga tttgttcttg tgcgcatttc tgcttcacct tcgaattgtt tatgcaagtc
    50461 ttcttctgta gaaccagtta attgatagta catttcagga gagatacctt gacgttgcat
    50521 gttgttcaag aattcgtcca ttgaacgatg tacttcatca tgaaccatca cgtgtggcaa
    50581 ttcaacgatt tctgcgtttt caacagccat acggatcgct gcttcatctt ttgcatcgtc
    50641 cgctgctttt tcttttgttt ctactaattc tttgcgatat ttttctttca attcgtctaa
    50701 tgttgatact tcgtcatcaa catcttttgc gaattcgtca tctaattcag gcacttcttt
    50761 tgcttttact tcatggattg tcactttgaa gactgcttct tttccagcaa gatcttctgc
    50821 ttgataatct tctgggaaag ttacatttac atctagactt tcacctgatt tatgaccaac
    50881 taattgatct tcaaaacctg gaatgaatga gcctgaacct aattcaagtg agtaattttc
    50941 gccttttccg ccatcaaacg cttcgccatc tacaaagcct tcgaagtcga ttactactgt
    51001 atcgccattt tcagctgcag catcttcttt gataactaat tcagcttgtg cttcttgttc
    51061 acgtttgata cgtgcatcca cgtcagcatc tgttacttca cggtcttgtt tttcaacaac
    51121 tagatcttta tattgtccta attttacttc tggtttcact gttacttcag cagtgattac
    51181 ccagtcttga cctttttcca tgctttcaac atcgattttt ggttgagaaa caggatcgat
    51241 gccagcttct ttgacagcag cttcataagc ttctggtaat acagcattca atgcatcttc
    51301 atataaagct tcttcaccgt acatacggtt gaatacttga cgagaaactt tccctttacg
    51361 gaatccaggt acgttcaagc tttttttcac tttattaaac gcagttgtta atcctttttg
    51421 gatttcgtct tgcgcaatcg taaattttaa aacaccatca ttcgtgcctt ttttttcaaa
    51481 tgttgcagtc atttaattcc ctccgagttt agaaattcat tttatacatc aaaaacgttt
    51541 catgcatacc tttaaatagt acactaaccc tttcaaattg taaacatatt gaatggatag
    51601 aatccttgtt tttcataaca tttttatctt tttcactgat aaaatctaat ggaagaagat
    51661 catttgctgc aaaatttttt ctcttttttt taaaaaatct tgtttttcat cttctgtacc
    51721 ttcatacatc gtttttcctg tacacgcctc tacagtcagt tgaaaccagc gcttcgggtc
    51781 ttcgatgtat gtatcataga caggataaac aattgcagat tgcaacgtga attcttcttt
    51841 taacacggaa ctaagcagcg catcttcatt cccgtaacga tctgaaaata cacataattg
    51901 ctggtaactt gcctgctgct gcggctttgg cagatctttt ggtatgactt tgactaactt
    51961 ttcttcaatc gtcaaataga aaacaggatg gcattcgttc aattgcctaa gtgtctccag
    52021 taattttgcc cttgctaaaa tatggacctc tggcgataca ataagtttac tcgctgtttg
    52081 gataaactca tgataaggaa gctgttcgat ctcatcaata gacgcaagct gacgtgcagg
    52141 ttccaacgaa ggaagttcct ccgatttatt gtggagtgcc ttgatcttct gcctctccat
    52201 ttgtccggat aattcttctg caaattggat tttttcaatg acccgctgct cgaaagcttc
    52261 ataaaaatct gtacttgcta tcaattttct agcatataaa aaatcttgtg tactgattaa
    52321 caagtcaaaa taaaattcag caaactcctc attttgaaga tactgttttt catgcaagag
    52381 tgcttgcttc aatgcttctt ttttctcgtc caactcgtaa agacaaaaaa caagcaggct
    52441 attagcctca aagctttctt tcaactcgta agctcgttga aataattctt ttgcctgctg
    52501 atattgctgg tttgctagtg cttgttgtgc cactcgcatt aaattctcat atttttctgg
    52561 aaaatcaatc ggcttcacgc agtgtcctcc tcttgctcat attcagctca tgttcaatag
    52621 tagcaaaaca aaaaaagcat gacaattaaa aataactgaa gcaccctcta aaagctagat
    52681 tttagatctg acttttagaa ggtgctttca actgttactg ctttttattg ctcatcagct
    52741 gttgacttcc tggttgaacg ctttgatcag attgatcgta acaacacttg tattagtgaa
    52801 aaaatcgccc agatgttgct ttttctgatt aaagactgct ggaagatacc ctaacatcag
    52861 caaaggaaat tttagaatga accggcaagc accttctcta actaaaacgg ttgaccagct
    52921 aagttcttct tcatcaaagc tgatgactcg aataccaaag atcattttcc caatcgtttg
    52981 gccatgattg aatttcgtca ataaaataaa atacgctaga taaataagta aggctgtgaa
    53041 tccgtagaga ctcagataag aatctgaaaa attaagccaa cctagatttt tggcaatacc
    53101 aagtgttaaa cgcgtaatag ctccgataca caaaagatcg accaagaagg caaacattcg
    53161 gataaaaaaa cctgcataga aatacttagg aaaatcattt gcttttggcg gttcatttct
    53221 tgtgtccgta tactttttcc atttacgttg tttctttttg atttcttcgg gcgtcaaagg
    53281 ctctctttca aaagagctca gtaccttttg ggaaaagttg gagtcaggtc ctacagcagg
    53341 ctgctgttgc ttatcttttt cttgttcctt attttcattt gaaagctctg acatgcttac
    53401 tcacctccgt aataatacat tgcttttggt gattccgctg tccctagatt ctcaagaacg
    53461 gataagatct gactagtctc agaaggcttc aatccctgga attctgctag tttacttcct
    53521 aaccatgtac tagcaaaccc agatggcgtg gaagaatatt ctactagttc cgcatcttca
    53581 agctgttgtt cctttcgtat agcttttaaa gcatcttcag gaaatcccaa ttcatcgact
    53641 aaacctactt cttttgcttg gacaccatca taaatccttc catcagcaat ttttttgact
    53701 tcttcttcag atttattccg tccctcgctg acgattgcta caaaacgact atatgcgctg
    53761 tcgatatatg cttgaagcac cgcttcttct tcttttgtct ctggtcgcgt tcctgaaccc
    53821 atatctttca acgctccgct tttaactgtt gtatcttcca cacccagttt ttctagaagt
    53881 cctgaataat tcaatcctga catgatcaca ccaattgagc cggtcactgt ttcttctgtt
    53941 gcaaagattt tatctgcttg agcagaaata tagtagccac cgctagctgc catgtttttc
    54001 atgctgacat acattgggat atcatgttct ttacgaatcg tatccaattt cttagcgatt
    54061 tccgcacttt cgtagacacc gccaccagga gaatttactt ctaaaaagat acctttgact
    54121 gttggatctt cttcaatcgc cttcagttgt tccataaaat ttttatgatt gtaaccttca
    54181 gaagcaaata aacctgaaga ccctcccgca gcaatcgttc catctactga taattttacg
    54241 attttttgcg tactgtctcc ctcttccaac acatttggag ctagttcttc atccccatat
    54301 aaccaactgt tcaatccctc aatttgatcg ttttcctgag gcttagtcaa actagctgaa
    54361 actaacgaaa ccacaaataa tcctgcagca atcagaactg ctgcccaacg tcttttattc
    54421 attctttctc ctttctaata caagtttttt atgacgatca cagtttctga ctgctcagaa
    54481 gtgtcgaacc gctctgcttt ttcttcccgc gcatacccgg aaaacgactc ttttgttaat
    54541 tcatcttcta ccgtaaactc acgagcttga gcaacttcca aggcatcagg tgcgatttct
    54601 tggatgctat cccagccagt atctaaaaat attttcgtat catctgatac accagctgat
    54661 gcttcgaatt tggacaattg tttttttaac attcccagtg tcatgatctc aatttttgaa
    54721 tccattttta ctcctcctct atccgattac tttctattgt accgaactcc tgaaaaagat
    54781 gatagataaa aacccattat tttttgacat aaaaaggaca tttgacaaag aatttgatat
    54841 ataccaattt attttttatt ctttttttgt tgacaagaac attcgcaaaa ggttatgatt
    54901 ttaaatgttt ccagataaaa aacaaaaagg aggatagtta taccttttag gatagcgcca
    54961 ggtctgtgta aacagatgac gaggagaggg tttatcgaaa gattcggcgg atggccctag
    55021 ggactgcact ctacagaaaa tccacaaaaa atgtctgcaa agataaaaca aaaggatttt
    55081 cctgcagctg gaagcattca tacgaattca ttatagaaag taggatgttt tttatgtgcg
    55141 gaattgttgg aatgattgga ttaaaaaatg taacaccagg attgattgac ggtctagaaa
    55201 aattagaata ccgtggttat gattcagctg gtatctttgt ctcagatggc acaactgatt
    55261 atctggtcaa agcacaaggc agaattcaaa atttgaaaaa caaaataaat attgatacta
    55321 caggaaatat aggtattggt catacacgat gggcaactca cgggcaacct tctgaagaaa
    55381 acgcccatcc acatacttcc caaagtggac gcttcgtgct cgttcacaac ggcgtaatcg
    55441 aaaactttga agaacttaaa attgcttatt tagcaaatga ccactttatt ggtgagaccg
    55501 atactgagat tatcgctcat ctaattgaaa ctttcgcgaa ggatactaca acgcaagagg
    55561 cgtttttaaa agctttgcgg gtcatcaaag gttcttacgc tttcgctttg attgatcgga
    55621 cagcacctga tgttatttac gtcgctaaaa ataaaagtcc actattaatc ggactcggcg
    55681 atggattcaa tgtgattgcc agcgatgcaa tggctatgct tgctcatacg aaggaattcg
    55741 ttgagattga agatgaagag atggttaccg tcacatcaga aaaagttatt attcaaaatt
    55801 ttgcaggtga catcgtagaa cgttcttcat tcgaagcaca agtagatgct tcagatatcg
    55861 agaaaggtac ctatcctttt tatatgctga aagaaatcga cgaacagcct attatcatgc
    55921 gtcgtatcgc tcaaaaatac gtaacagaag acaatcgtgt ctctcttgat caaaagctgg
    55981 tcgatacatt gtctgatagc gatcgaatct atatcgttgc ttgcggaact agttaccatg
    56041 cggggttagc aggtaaacag actttggaaa aattgacaca gattcctgtt gaggttcatt
    56101 tagccagtga atttggctat aacacacctc ttctttcaaa aaaaccattc tttattttct
    56161 tgagtcaaag tggcgaaaca gcagacagcc gccaagtgct ggtaaaaatc aaccgtctcg
    56221 gctacccttc acttacgatt acaaatgttg ctggttctac tctttcgcgg gaagcatcct
    56281 ttacactgct attgcatgct ggacccgaaa tcgcagtagc atcaacgaaa gcttatactg
    56341 cacaaatagc tgtactaact ttattagcca aagctatcgg tgataaaaaa gaatttgaaa
    56401 cttctcttgt attcgatatc actcatgaat tgagtttagt tgctaatgcg atggaatcgg
    56461 tcatcgccca aaaagattat ttagaagagt tggcagaagt atatctcagc gatacaagaa
    56521 atgcctttta cattggacgt ggtgctgact acaacgtttc tcttgaagct gctttgaaat
    56581 tgaaagaaat cagttatatc caggcagaag gttttgccgc tggcgaattg aaacatggaa
    56641 cgattgcttt gatcgaagaa ggaacaccag tgatcggaat catcagtgaa gaagtgactg
    56701 gtgcgcatac tcgaggaaac ctgaaagaag tcgaaagccg tggcgctaaa acactagtta
    56761 ttgtctcaga gggattagaa aaggaaagtg accagctggt gctaccaact gtccaccctt
    56821 atctaactac tctaaccaca gtcgtaccga ctcaattact tgcatattac gcaacgctat
    56881 tacggggatt cgacgttgac aaaccacgta atttagcgaa atcagtgaca gtagaataat
    56941 aaaagaacaa ggaccagtgt cagacatctg acactggtcc ttgttctttt attttttatg
    57001 aaaatatctc ttaatccagt ccgatttctt ctttgactac tgccgcgatt ttatcgacat
    57061 agtaattaac tttctcatca gtaggcgctt ctgccatcac tcgaagtaat ggttctgtac
    57121 cggaaggacg aactaggatt cttccttctg atcccatttc agcttctgcc tcttcaatca
    57181 cttttttgat tgccggaaca tccatcgcac cgtttttatc acttacgcgg atattcacta
    57241 atttttgcgg atagattgtt acctctgctg caagctctga taatttctta cctgtctgtt
    57301 tcatgatatt cagtaactga attcctgaaa gcatcccatc tccagttgtg ttaaaatcta
    57361 agaaaatcat gtgcccagac tgctcgccgc caaagttata atcgtttttg cgcatttctt
    57421 ctacaacgta acgatcacca acttgtgtaa cgacatcttt caaaccgatt ccttccaccg
    57481 ctttgtggaa tcctaagtta ctcatgacag tcgtaacgat cgtatctttt ttcaatcgat
    57541 ttttttctgc caagtattta gcacagataa acatgatttt atcaccgtcg acaatgtttc
    57601 caagttcatc caccgcaata atacgatcgc catcgccatc gaaagctagt ccagcatctg
    57661 ctcctttttc aacgaccatc tcagctaaac gttctggatg ggtagagcct acgccgtcat
    57721 tgatatttaa cccgtctgga tttgtgccca ttgtataaaa atctgtttca agatcggcaa
    57781 acaaacgatt aacacttgtt gaagttgctc cgtttgctgc atcgacacaa atggtcaaac
    57841 cggaaaggtc accgttgatc gtttgtacaa ggaattgaga atatttcaat aagccttctg
    57901 gaaattcttc tacagttccc aatccttcag ctgatggacg aggcaattca tccacttcag
    57961 catctaataa tgcttcaatt tctagctctt ggtcatctac tagtttaaaa ccatctgcac
    58021 cgaaaaattt gattccatta tcttgtgctg gattatgaga agcagaaatc atgacccctg
    58081 cgcttgcttt ttgcaaacgt gtcagataag caacacctgg tgtagagatc acacctagct
    58141 ggaacacttc gatacccacc gataaaagtc ctgaaatcag cgcttcttct aacatttgac
    58201 ctgagattct tgtatcccgc cctactaaca cgcgtgggcg ttttccttct tcttcatgct
    58261 ggcttaggac ataacctccg caccgtccca gcttaaatgc aagttctggc gtaagttcct
    58321 tgttggcttc accgcgtacg ccgtctgttc caaaatattt acccatagtt gtaaaattac
    58381 tcctttaact taattttctg aggaatttgt tctgctcatt tgtgttgatt cgcttgtttc
    58441 tgatgtttct tctgtacttg tggtcgagct gcttgtccca gattccgaac tggaagaaat
    58501 ggttgttgtc gatggttccg ttcctgaatc agcagatgaa atacttctac tcgtacttgt
    58561 attttcttgt gtccgactgc tcgtcgaagc agagctactg cttgacgttt gcaacacggg
    58621 tgtaatctcc acactaactg attcgggctt gatacttaac ccctcttcta ctggtatttc
    58681 aattttacga gttgttcgat gattgatacc tgagacattg atcggtacag caatactgtc
    58741 accgatcgat gcaagcaaat cgctagagcc gcttacttct gcttccagtt ttgaaaaact
    58801 gaaatcatag ctttctaccc cggcagctgg tgttccttgc tgtatcccat aaagtcgtac
    58861 cgacttgctt ggcttgatcg aatcagctct cactgttact tgcactggat cagagacgat
    58921 acttaatact tcccctgctg cattcagcgc ctgtattggg acctcactat taatcccatc
    58981 atctgtgacc atcgaagcgt ccacatcagc tactactcgt ttgatttctg ccaatgtttt
    59041 gtcacctgta gtgatagaga cttttttagg actaacggaa gtatcatcaa gttcatatcc
    59101 agatggtgtg aacgttgaag gaatcaacgt ttccacatcg aaatctttta ttaccttttt
    59161 ctcaaccgtt actgtaatag tcgatggttc gatctttgct gtgacagcac tgctcaaatt
    59221 ttgaattttt agcggaactt catgtgttcc ttcgcccagc ttagtgatat ctgctgtgac
    59281 gcggaacatc cgtgtatctt cgtttgcctc tgcattcaac tgaactcggt ttgcactgga
    59341 aagtttgaca gtcactgcat tttcatatcc atggatatag tagcgctttg aatcataaga
    59401 aatcttgatc ggcacccccg taactgtttg attatacgct tcatttccag aaagcgtatt
    59461 tggaacggac tggccatttg cattgaagta cagcgcaata ctgaacagca atgcaataaa
    59521 actgtaaaga atgcttgtcc gtttctcttt tgagatcatt tgcgatcccc tcccttagtc
    59581 ataccgtcta ggaaagattg aatcacgttt ttcttgtttt ttgttttctc ttcagcaatt
    59641 agttctctac gcaagattgc caaatattct tgctgggata agccagccat cagttcatta
    59701 tctaacgtga tgctgacgtc cccggtttct tcagagacaa cgatggtaat cgcgtcactg
    59761 acttcgctta taccgacagc tgcccggtgt cgagtcccga attctttagg gatcaacata
    59821 ctttctgata gaggaagata ggcgcttgct actgcgatct ttccttcttt tacaataact
    59881 gcaccgtcat gcagcggtgt attaggaata aagatattga tcagcagctc cccagtgata
    59941 tctgcatcta aagcaatccc tgtctcaata tactcatcta atccagtatg acgttcgata
    60001 gtgatcaacg caccgatttt tcgcttggac atatattgga tggctttgtc aaaagacaag
    60061 atcatcttct catcttcctg ctgttcgctt tttgattgtt tgaaaagcga actgcgtcca
    60121 agatgttcca gtcctctccg gatttccggc tgaaaaatca cgatagcagc tatcaccccg
    60181 tacgtaatga cttggttcat cagccaagac aaggtgtgca gaccaatgat ttccgctacg
    60241 attcggataa caataaaaat agctacacct ttcagaagct gaatcgcttt tgtgcctcgc
    60301 agcagttgga tcagtttgta tacaagatac cagacaacca gaatatcaag tatgttcacc
    60361 aaaaaattca gagagaacaa gtcaccggaa aacatttgct gccaatagtc caaattaaaa
    60421 agttccccga tttgaaatga cataaaaagc ctctcttctc ttttgagatt tcctctctag
    60481 tatatcatat gttttaattt tagaggctga tatgctcgta tttttcttga gaaaaacgca
    60541 taattttcaa gaaaattgca agtatgcgtt ttatttttct ctgcaataaa tatttttaaa
    60601 cttcagagaa ttattcatta tattttttct gttatttttc ctttttttct tttgtagctt
    60661 gttccatttt tttacgtcgt tctgctctgg cagctcttct agcagctctt ctttcttctg
    60721 cttcaaggtc cgtcgttttt tcttttctag tcttaacggg tacatcgata gattcctctg
    60781 gttcaagtcg tttcttgatt ttttctaatt ctcccgtcag tgcagcatag ttgtaaaaac
    60841 gtgttttagc gtcgtggaca gtcttttcaa ataaagcttt tgcaagattt ggatttgcgt
    60901 tatctagcga ggaaaagcga acttgtttcc tcatgaaatc aggcatagca gagaaatcag
    60961 gttttttgta gtccaaagtc attgggttct tgtttttttc gaccattgta gggttatatc
    61021 gatataatga ccaataacca gaagaaacag cttcttttgc ttctttaagc gattctttca
    61081 ttccgccaag caagccatgt gtgatacaag gcgtataggc aataatcaga gaaggaccag
    61141 gatacttttc tgcttcttca aatgccttga tcgtttgcat ttgattggca cctgaagcaa
    61201 tttgcgccac ataaacattt ccatatgtca tcgccatcat tcctagatct ttctttgaag
    61261 catactttcc actagccgaa aatttagcga ttgccgaagc tggtgttgct tttgaggttt
    61321 gaccgccagt attggaatag acttcgttat ccaaaacaag gatattcacg tctgctccgc
    61381 tggcaagtac atgatcgatc ccgccaaaac caatgtcgta agcccatccg tctccaccaa
    61441 tcatccattg gctgtttttt acgaatagat cctttctctt gtatatatcc tccagttcag
    61501 ggaaaattga tcgttcgctt tcgatcgcag cttgcagttt tgctgctctt tgctgcgttc
    61561 cttcactctc aaacatgtgt actaaccagt cttccatcaa ttctcgtagt tcttctgata
    61621 cttttggcaa tacttctttg attgaacttg ccaaagcctc gcgacgagtc tgtgccgcta
    61681 gaagcatacc ataaccaaat tcagcattat cttccaataa agaatttgac catgcaggcc
    61741 cttgaccaca ttcattggtc gtataaggag aagcaggaga agcaccgccc caaatagaag
    61801 aacagcctgt cgcattcgcg atcatcattc gatcaccgaa catttgtgtc aataatttta
    61861 cataaggtgt ttcgccacag cctgagcaag cgcccgagaa ttcaagaagc ggttgattga
    61921 attgtgtaga caagacagta ttctttcctt tgactggatt tgctttttgt cttaatgtca
    61981 ttgcaaaagc ccaattcaca gcttgttctt tctgttcttc ataaggcttc atcactaaag
    62041 ctttttcttt tgcgggacaa gcctctacac acagaccaca acctgtacag tcttccactg
    62101 aaacttggat acggtatttt acgccgtctg cgcctcgcat ttctctcacg ataaatcctt
    62161 ctggcgcttc ttccatttcc tcttcatctg ctaaaaatgg acggatggca gcatgaggac
    62221 aaacaaaggc acattcatta cacatcgtac agcgatcact aatccactca ggtacttcta
    62281 aagcgacgcc ccgcttttcc atagaagccg ttcctaaagg catttcgcca gttgtcattc
    62341 catttgctac aagtgtacca acagagagtt gatttccttc ctgtgcattc actggttcaa
    62401 gtatttcttg tatataacca gtgttccttt tattgatttt ttcaggcatc tccaacgttc
    62461 tccaatggtc tgggatcgcg acttgatgaa gcagttcggt cgtccgatcg attgcttgta
    62521 tgtttttttc tacgatagcc attgatttat gtccatagct tttcagggct tcttctttta
    62581 gcagcggaag cacctcatca aaaggaatga tgtcagctaa tttaaaaaaa gccgtttcca
    62641 ttgccgtatt gatccggcgt cccaaaccga cttcttgggc caatctcatg gcattgatcg
    62701 tatagaatct aatgttattt tcagccaagt aacgttttaa ttttaagggc agatgcgttt
    62761 ctaattgttc gtcgctccaa agagtattca ataaaaaaat gccacctggc ttcaatcctt
    62821 tgaccaagtc atagctatgc aggtaagctg gtgtgtgaca ggcaacaagg tccgcatgct
    62881 cgacaagata cgctgaacgt atcggcgtat ctccaaaacg aagatgagag acagtcagac
    62941 cgccagattt tttcgaatca taataaaaat agccttgcgc atatttatcg gtgtgatctc
    63001 caataatctt gatagctgat ttatttgctc caactgttcc gtcagatcca aatccccaaa
    63061 acttggcctg gaatgtttgc ggttctgtca aatccagact ttcctttggt tccaaagatt
    63121 gataagtcac atcatccacg atccctatgg taaaccgttt tttgcgtatg cttgggtctt
    63181 ttttcaattc atcaaaaact gctgagattt ggtcgggagt cacatcttta gaccccaatc
    63241 cgtagcgacc accaataaca gccgggcgaa gttcagaatc atataacgca ctttgaacgt
    63301 ctaataagag aggttctcca cctgcccctg gttctttact tcgatcaaga acagcaattg
    63361 tttttactgt atgaggcaac ttttgcacga atgtttccac tggaaaagga cgataaagat
    63421 ggatattcaa aaaacctgtt ttccgtccgt ttgcattcaa gtaatcaatc gtttgttcaa
    63481 tcgtctgtgc aactgatccc atagaaacaa tgacttcttc cgcatcgtct gctccgtaat
    63541 aagtcaccaa gtcataagaa gttcctcgta atcggttgat ctctttcata tatttttgaa
    63601 cgatttcagg taatttatca taataggggt tgatcgtttc ccgttgttga aaatggatgt
    63661 ctggattctg gttcgtacca ctaacagtcg gatgattggg attcatccct cgtttacgaa
    63721 atgaagctaa tttctcctga ttgatcagag gagctaattc ttcatattcc aaaacttcga
    63781 ttttttgaat ctcgtgactc gtacggaatc catcaaagaa attcaaaaaa gggacttctc
    63841 cttcaataga agcaagatgg gcaacaggtg ctaaatccat cacttcttgt acactgcttt
    63901 cagctaacat agcaaatcct gtttgtcttg cagccatcac gtctccgtga tctccgaaaa
    63961 tattcaacgc atttgtcgtc actgcccgac tagcaacgtg gaaaacactt ggcagtagtt
    64021 ccccagcaat cttgtacata ttcggtatca tcaaaagcag cccttgtgaa gcggtatatg
    64081 tagttgtcag tgcaccagtt ttcaatgatc catggaccac accagctgct cctgcttcgg
    64141 attgcatttc tactactttt actggttgac cgaatacgtt ttcttttcct gctgctgacc
    64201 attgatctac taattcagcc atggtcgaac tgggtgttat tggatatatt gcagcaatct
    64261 cggtaaatgc ataagaaata tatgcggctg ccgtattgcc atccattgtt ttcatttttc
    64321 gcatgattca tcatccttgt tcatattctc tcttattatc tgttccatta taacgtaaaa
    64381 catgccattt ttttctttta tttataaaaa tattttacat gtatctattt gaattggtca
    64441 tctaattttt agacaaacag tctaaatcgt tgcctttttt tgtcagacat gtataaacta
    64501 taagcgaaag aggtgtttta acatggatat atcagtgatt gatgcaacga aaacaaatac
    64561 acaaaaagga atcctttatg gttcatcgaa tgctcctaaa aaaatggtcg aattcatcaa
    64621 cttagcttgt ccgtattgcc gacaatggtt tgaagaatct tatgagctat tagaagaagc
    64681 agtccaaagc gggcaattgc agcgagtgat caaattgttc gataaagaaa aagaaagctt
    64741 acagcgaggg aacgtgatgc accggtatct cacaatcagc gatggccaaa aagcgatcaa
    64801 agagatcaaa caaatattcg atactcaaga cgagtggaaa catctttctc tccaagaagt
    64861 agcggatttt gcagtagata aactaaagct gacagaacaa aaagacgaac agctgagcca
    64921 agctgtcatc aacgaagctg agcaagcaca tattcgattt gttccaactg tcatcttggg
    64981 taaagaaatc tttgatgaat ctatttcaat agaggaatta aaagaattga tccaaacaaa
    65041 ataaaaaaat aaggagcgtg ggacaaaagt aaagaacact tttgtctcac gctctaaaca
    65101 cgtataaacg gcggaagcag aagcaactcc ttcttatttc ttggagttaa acgcttctgt
    65161 cccagcctcc ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca
    65221 tttcttatga caagctaagc aaattatctc caccaaataa tttattcata tttttgactt
    65281 gcgcatacta actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta
    65341 atatttatgt acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc
    65401 aattctactt caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg
    65461 ttgagctagg tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac
    65521 caaacgcaag atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat
    65581 aaagctaaca actattaact cctctcagct aatagttgct ctctttttta ttcagattca
    65641 tttttgctga tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat
    65701 tacaacattc actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc
    65761 tattggtgga tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc
    65821 taaaccactt ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt
    65881 tacgccatta aatggatacc aattaggatt aggctctctt tttttctgta catctcccat
    65941 agaatagtta gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa
    66001 taagatattt cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc
    66061 catgctacct gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac
    66121 aatcctttcc ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc
    66181 tggtcttggt ggcttaggac ccaatattat tctacttaaa accgcattcg ttctcagatg
    66241 aaacacagct ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat
    66301 tattcgcaca tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa
    66361 atatctactg gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca
    66421 aatcatcagc ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct
    66481 aatcaccaaa attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt
    66541 attgtgtcaa aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt
    66601 ctcctaatat aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat
    66661 gtggttttaa tgacattgat tgtacatttt cccccatata atatcgcgca cttcctcgac
    66721 taaaatagat tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac
    66781 ttctccatct tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag
    66841 ctatgacagt atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg
    66901 gaaattctcc ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag
    66961 gaaatattaa gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa
    67021 tccaaaatat aatttgtaca attctcattg atccattgta ttccatttta ttccccctat
    67081 tactattcat atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac
    67141 cttgattttt tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt
    67201 gtctatctaa catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat
    67261 gcatctccct cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca
    67321 taagcagata aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact
    67381 gaatttgctg cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga
    67441 tttacgtgga cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga
    67501 actacgcaac aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg
    67561 aatataaaaa ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca
    67621 ttatactgta tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt
    67681 atcaatctat tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc
    67741 ctatgaatct cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta
    67801 attttaccca ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga
    67861 tgatcatttt aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat
    67921 cgcatctagc ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt
    67981 tcggtctcag aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg
    68041 ttgccgatga ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt
    68101 gcagatacca atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct
    68161 taccattaga tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta
    68221 ttttatgaat tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc
    68281 tgataaacca ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt
    68341 ccgagaacga aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg
    68401 gacttagtgt gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta
    68461 aggctatact gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta
    68521 ataatatgaa aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat
    68581 ttttatacaa tgaccattta tttataggct tttctttcat tttacttccc cctactgttc
    68641 tgttcacacg gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac
    68701 ttgctaacta tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta
    68761 ttcatctcat tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa
    68821 gaattattta gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct
    68881 atgatgaaaa gccttggaat acagtagctc ttaattggta atactttttc cattataaat
    68941 aacctctttt cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa
    69001 cttatatttc cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag
    69061 cattacgcct gccagaataa accaattatt tgt
//
LOCUS       DAFEOM010000008        67760 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.008, whole genome shotgun sequence.
ACCESSION   DAFEOM010000008
VERSION     DAFEOM010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 67760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.008"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(98..1228)
                     /locus_tag="LP524_000622"
     CDS             complement(98..1228)
                     /locus_tag="LP524_000622"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBL2233698.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="LP524_000623"
     CDS             complement(1877..3079)
                     /locus_tag="LP524_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL2233699.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="LP524_000624"
     CDS             complement(3139..3342)
                     /locus_tag="LP524_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HBL2233700.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEQLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="LP524_000625"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="LP524_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4879..5691
                     /locus_tag="LP524_000626"
     CDS             4879..5691
                     /locus_tag="LP524_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2233701.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6715..6867)
                     /locus_tag="LP524_000627"
     CDS             complement(6715..6867)
                     /locus_tag="LP524_000627"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBL2233702.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7058..7936)
                     /locus_tag="LP524_000628"
     CDS             complement(7058..7936)
                     /locus_tag="LP524_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL2233703.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7975..9093)
                     /locus_tag="LP524_000629"
     CDS             complement(7975..9093)
                     /locus_tag="LP524_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HBL2233704.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9090..11189)
                     /locus_tag="LP524_000630"
     CDS             complement(9090..11189)
                     /locus_tag="LP524_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HBL2233705.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11240..12061)
                     /locus_tag="LP524_000631"
     CDS             complement(11240..12061)
                     /locus_tag="LP524_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL2233706.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12058..12867)
                     /locus_tag="LP524_000632"
     CDS             complement(12058..12867)
                     /locus_tag="LP524_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL2233707.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12888..13382)
                     /locus_tag="LP524_000633"
     CDS             complement(12888..13382)
                     /locus_tag="LP524_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2233708.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13394..13882)
                     /locus_tag="LP524_000634"
     CDS             complement(13394..13882)
                     /locus_tag="LP524_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL2233709.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13959..16655)
                     /locus_tag="LP524_000635"
     CDS             complement(13959..16655)
                     /locus_tag="LP524_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL2233710.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17083..18156)
                     /locus_tag="LP524_000636"
     CDS             complement(17083..18156)
                     /locus_tag="LP524_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL2233711.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18153..18980)
                     /locus_tag="LP524_000637"
     CDS             complement(18153..18980)
                     /locus_tag="LP524_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2233712.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18981..19787)
                     /locus_tag="LP524_000638"
     CDS             complement(18981..19787)
                     /locus_tag="LP524_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2233713.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19799..20884)
                     /locus_tag="LP524_000639"
     CDS             complement(19799..20884)
                     /locus_tag="LP524_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2233714.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20901..21443)
                     /locus_tag="LP524_000640"
     CDS             complement(20901..21443)
                     /locus_tag="LP524_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2233715.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21701..22690
                     /locus_tag="LP524_000641"
     CDS             21701..22690
                     /locus_tag="LP524_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBL2233716.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22687..23424
                     /locus_tag="LP524_000642"
     CDS             22687..23424
                     /locus_tag="LP524_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HBL2233717.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23421..23972
                     /gene="coaC"
                     /locus_tag="LP524_000643"
     CDS             23421..23972
                     /gene="coaC"
                     /locus_tag="LP524_000643"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HBL2233718.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23988..24620
                     /locus_tag="LP524_000644"
     CDS             23988..24620
                     /locus_tag="LP524_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL2233719.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24705..25352
                     /locus_tag="LP524_000645"
     CDS             24705..25352
                     /locus_tag="LP524_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL2233720.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25357..26340
                     /locus_tag="LP524_000646"
                     /pseudo
     CDS             25357..26340
                     /locus_tag="LP524_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
     gene            complement(26743..28257)
                     /locus_tag="LP524_000647"
     CDS             complement(26743..28257)
                     /locus_tag="LP524_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HBL2233721.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            28395..29201
                     /locus_tag="LP524_000648"
     CDS             28395..29201
                     /locus_tag="LP524_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233722.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            complement(29376..29945)
                     /locus_tag="LP524_000649"
     CDS             complement(29376..29945)
                     /locus_tag="LP524_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2233723.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(30028..32088)
                     /locus_tag="LP524_000650"
     CDS             complement(30028..32088)
                     /locus_tag="LP524_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HBL2233724.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            complement(32365..33843)
                     /locus_tag="LP524_000651"
     CDS             complement(32365..33843)
                     /locus_tag="LP524_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2233725.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(34141..35076)
                     /gene="murB"
                     /locus_tag="LP524_000652"
     CDS             complement(34141..35076)
                     /gene="murB"
                     /locus_tag="LP524_000652"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBL2233726.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(35149..36069)
                     /locus_tag="LP524_000653"
     CDS             complement(35149..36069)
                     /locus_tag="LP524_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL2233727.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            36182..36934
                     /gene="xth"
                     /locus_tag="LP524_000654"
     CDS             36182..36934
                     /gene="xth"
                     /locus_tag="LP524_000654"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBL2233728.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            37798..38331
                     /locus_tag="LP524_000655"
     CDS             37798..38331
                     /locus_tag="LP524_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBL2233729.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(38821..39345)
                     /locus_tag="LP524_000656"
     CDS             complement(38821..39345)
                     /locus_tag="LP524_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2233730.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(39338..39811)
                     /gene="tsaE"
                     /locus_tag="LP524_000657"
     CDS             complement(39338..39811)
                     /gene="tsaE"
                     /locus_tag="LP524_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBL2233731.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(39934..40914)
                     /gene="pta"
                     /locus_tag="LP524_000658"
     CDS             complement(39934..40914)
                     /gene="pta"
                     /locus_tag="LP524_000658"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBL2233732.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(41067..41747)
                     /locus_tag="LP524_000659"
     CDS             complement(41067..41747)
                     /locus_tag="LP524_000659"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBL2233733.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            41873..42742
                     /locus_tag="LP524_000660"
     CDS             41873..42742
                     /locus_tag="LP524_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL2233734.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            42768..43283
                     /locus_tag="LP524_000661"
     CDS             42768..43283
                     /locus_tag="LP524_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233735.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(43330..43923)
                     /locus_tag="LP524_000662"
     CDS             complement(43330..43923)
                     /locus_tag="LP524_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2233736.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(43948..44127)
                     /locus_tag="LP524_000663"
     CDS             complement(43948..44127)
                     /locus_tag="LP524_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233737.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(44204..44713)
                     /locus_tag="LP524_000664"
     CDS             complement(44204..44713)
                     /locus_tag="LP524_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233738.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(44809..45189)
                     /locus_tag="LP524_000665"
     CDS             complement(44809..45189)
                     /locus_tag="LP524_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL2233739.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            45519..46268
                     /locus_tag="LP524_000666"
     CDS             45519..46268
                     /locus_tag="LP524_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HBL2233740.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(46452..47729)
                     /locus_tag="LP524_000667"
     CDS             complement(46452..47729)
                     /locus_tag="LP524_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233741.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCHYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(47808..48452)
                     /gene="pcp"
                     /locus_tag="LP524_000668"
     CDS             complement(47808..48452)
                     /gene="pcp"
                     /locus_tag="LP524_000668"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBL2233742.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(48651..49595)
                     /locus_tag="LP524_000669"
     CDS             complement(48651..49595)
                     /locus_tag="LP524_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBL2233743.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(49597..50283)
                     /locus_tag="LP524_000670"
     CDS             complement(49597..50283)
                     /locus_tag="LP524_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBL2233744.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            50757..51077
                     /locus_tag="LP524_000671"
     CDS             50757..51077
                     /locus_tag="LP524_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HBL2233745.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            51231..52136
                     /locus_tag="LP524_000672"
     CDS             51231..52136
                     /locus_tag="LP524_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL2233746.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            52133..52906
                     /locus_tag="LP524_000673"
     CDS             52133..52906
                     /locus_tag="LP524_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBL2233747.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(53087..54511)
                     /locus_tag="LP524_000674"
     CDS             complement(53087..54511)
                     /locus_tag="LP524_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL2233748.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            54722..55168
                     /gene="nrdI"
                     /locus_tag="LP524_000675"
     CDS             54722..55168
                     /gene="nrdI"
                     /locus_tag="LP524_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBL2233749.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(55233..57215)
                     /locus_tag="LP524_000676"
     CDS             complement(55233..57215)
                     /locus_tag="LP524_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL2233750.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(57229..58125)
                     /gene="murQ"
                     /locus_tag="LP524_000677"
     CDS             complement(57229..58125)
                     /gene="murQ"
                     /locus_tag="LP524_000677"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBL2233751.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(58128..59189)
                     /locus_tag="LP524_000678"
     CDS             complement(58128..59189)
                     /locus_tag="LP524_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBL2233752.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(59400..59966)
                     /locus_tag="LP524_000679"
     CDS             complement(59400..59966)
                     /locus_tag="LP524_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBL2233753.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(59994..61496)
                     /gene="eat(A)"
                     /locus_tag="LP524_000680"
     CDS             complement(59994..61496)
                     /gene="eat(A)"
                     /locus_tag="LP524_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBL2233754.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(61754..62875)
                     /locus_tag="LP524_000681"
     CDS             complement(61754..62875)
                     /locus_tag="LP524_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBL2233755.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            63001..63942
                     /locus_tag="LP524_000682"
     CDS             63001..63942
                     /locus_tag="LP524_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBL2233756.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(64092..64988)
                     /locus_tag="LP524_000683"
     CDS             complement(64092..64988)
                     /locus_tag="LP524_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBL2233757.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            65257..66597
                     /locus_tag="LP524_000684"
     CDS             65257..66597
                     /locus_tag="LP524_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL2233758.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            66815..67258
                     /locus_tag="LP524_000685"
     CDS             66815..67258
                     /locus_tag="LP524_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HBL2233759.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
BASE COUNT        19976 a        13995 c        11788 g        22001 t
ORIGIN      
        1 aataaataat ataaaaggta aataaaaaag agtggatttc tccactccat gaaaagccga
       61 ctttttggga cgcttttttt tgccaagcct cacattttta tagccaaact tcttttgcga
      121 cttgctgcaa taattggatt ttcttccatt gttcttcttc tgttaaatgg tttccttctt
      181 ctgttgaagc aaacccacat tgaggactga tacttagatt ttccaatggt acaaattgtt
      241 gtgcttcttt tattcgttct tttattttct ccgtatcttc caaatctgga aacttagaag
      301 taactaatcc taaaacaact ttactttctt tgttactcca atgtcttaaa ggagtaaaat
      361 ctcctgctcg gtcagaatcg tattccaaaa agaaaccatc ataatttgtt gaaaacaact
      421 cttctgcaat gggttcatat ccaccggaaa acagccaaga agaagcgtga ttcccgcggc
      481 agatatgtgt tgtgatcgtc aaatcttctg gtttgtcttc cagtgcgtta ttgataatct
      541 ctgtacaaat atgggccatt tctttcggat caattccttt ttgaagaagt gcttctcttt
      601 tagtttgatc agctagaaac gcccaatttg tatcatcgaa ttgcaggtaa cggcaacctt
      661 tttcataaaa agcatgaatc gctgctttgt aagtagagga caagtcctca taaaaatcag
      721 atagatcagg ataaatctcc ttgatttttg aagaaccatc attagatagg atctcttgtc
      781 gtaaaatcat cgtgggacta gggatcgttg cttttgcaac aaaagtatca tctgtttcca
      841 ctacttcttt cagaaaagca aaatctttta aaaatgggtg atttggattg aaagataatt
      901 tatctactac ccgaatatta taagctggtg ctgcttttcc gtggaatgtt tgattatatc
      961 cagtttctgg aacatagcct tcgataccat ttagttcttc taaaaaatca agatgccaaa
     1021 atccacgccg aaattcacca tctgtaattc ctttgatgcc gactgctttt tggtcttcca
     1081 ctaatttttt tatttcctgg tcttcgattg cacgaagttc ttcttttgaa agtaagcctt
     1141 cttgaacttt ttttcttgct tctttgatcg attgcgggcg taagaaactt ccgacatgat
     1201 ctactgtaaa tggtctattt ttgctcattt cactcactct tttctttgtt ttttgtaaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagctgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta gaaccggggg ctttattcct gcagtccggc
     5821 tacgccttcc tgcaatagac aaaactccag atcgggtaat tcgcttgtgg cgaaacccga
     5881 cctggagttt gtctctgtat tttgaaccct aaaaattaat caaacagact gatattctca
     5941 taatcagcgc gaaataagcc attaagtaca gttttcctac ctatttttga taacccgctg
     6001 gttagttaat attccttttc cgagagagat aaatgattgg ttaaatctat atacatctca
     6061 acctttaaaa atgttttcga aatacttcat cagtacatgt catacaaatt accagtgcat
     6121 tctcaaattc tagttcttta attgatttca gttgaggagc agttactttt ggcggaaatt
     6181 tccatttttg tccaaacctc ttcccccgat gaaatttctt tgttgcaatt attgcaatga
     6241 aaaatttttg tttcaaaaat atacataatt acttcctcca ataaaaacta tctacctctc
     6301 aaattatttt tctctaataa gaaaaacaga aaaggtaata agatacaaca gtgatgattc
     6361 ctaataaaga aaagactaca ataatatttt tctaacttat cgccttcaaa gaaatagttt
     6421 cttatttttc actaaaataa aaaatttatt gcaaaaaaaa tagaagtgac aaaattatca
     6481 atcatttcaa ggtaatataa ctacctgagt ttattatcga ttaaaatgct aactacattt
     6541 gcaagagaag cgagagttaa aggtaaatat agaatttcta cttttttagc aatagaaata
     6601 ggaataaaat atttaaaatg tatagaaact ttcttttttt ttcttgctat ctttttataa
     6661 tcacaactct ctataataga attacgattt attttatacg gctgaaaaaa tctatcacca
     6721 aaaaaattca aacattaagc caaggattat gaagtcaaca tttcgaaaaa tgaacataca
     6781 ttggtaagca actataacta ttaccacaga cataaagtaa cacaatatat tactatttaa
     6841 gtgtacgcca ccagtgaata tcctcaaggt atacagcgca caacaaaaag aaaaaaggaa
     6901 acgcagaagc atttcctttc agtgatttaa tgaaaacaac acgatttgac ttagagcctt
     6961 tttatttgaa ttcattttcg ctttatccaa cttcatttga tcatttgatt actcggttaa
     7021 atacttttga catgagtatc aatgaaacat tcattaatta tggttgctta tttcttaccg
     7081 cttttctggc caatgctaat ccgccgatta ttccggcatt atcacctaat tcgggcgtaa
     7141 taatgtagct atcaattggt ggtatttcaa catatccatt tataagctca acaaatttat
     7201 cctgaacttt tttcttcatg tgttcttgtt tcattacacc accgccaaaa ataattctat
     7261 ctggcgaaaa catcagtgtt gtattgtatg cacattgggc aaggtaaaag gcttcaatct
     7321 gccaaaacga atcgtcagcc aataaattct ggccttttac acccaaccgt ttttcaatcg
     7381 ctggtcctgc tgccatcccc tctaaacaat cttggtggaa gggacagttt cctgaaaaat
     7441 tgtcctttgg atgtcgcttg atcaacatat gccccatttc cggatgactg aacccttcca
     7501 cgaaagtccc attgaacaag gcaccacctc cgatacctgt tcccacagtg aagtatgcaa
     7561 cacctgctaa ccctttgcca gaaccagcaa catattcacc atagcaagcc gcattgacat
     7621 ctgttgtcca ttcaatcgga atattgaaat attgctttag cgttcctaca aaattgaaat
     7681 tttgccaagc aagttttggt gttgatgtga tatgaccgaa actcgttgat ttgcattgaa
     7741 tatcaattgg accaaagcag ccgaccccga tgcctgccaa ttgttcctta aaaggtttaa
     7801 aaaaatcaat gacctgcttc attgtttcaa ctggagttgt tgtgggaaaa ctgactcttt
     7861 ccaaaatcgt taattcctca gtaccgactg cacagacaaa tttagtccct ccagcttcaa
     7921 ttgccccata tatcatcata ggttcctccc ttcgaatatg tgaaaaacac ttcattaacg
     7981 cggcaagcgt tctcgatcag tgaaatgacg tttagtattt cctgaagctt ctgttgaata
     8041 ccagtaagcg acactagcaa catcgtcttg ccgctcaaat aagcgaacat catcgttgcc
     8101 gatttgttgt aaagtgaccg ataaatcttc ggtaaaacag atggggtctt gaagatgcca
     8161 acgataaaga ccatgtcgtg gcattgcgtc gtcaccaaag gcatgaacgg cattcaattt
     8221 tcccgtttca aaacgatctc ttgtggcatc tctatttgat tggtaaggat accctaaaaa
     8281 ggctgttgaa tagtttttcg catgtggacg tccgaactca tcgtactcat gaaaggccca
     8341 cgccccgcca aaataatctt ctgaacctgt tgaggttacg gtcggatact cactatcccc
     8401 atcaatgtag aatttaaact cgccttcgcc ccaccaataa cgttctaggc aggtaagcgc
     8461 aaggtaagta cccacgtaat agccttgacc ctttaatcga tcaattaact tataatcctc
     8521 ctgtaatttg gtaatgcgtt cccgatgcca atttgcgtga aaatataggc tattttcttc
     8581 caattcgtcc accaaagcgt aatttatcgt ataaaaaaat gaggacatat ccttcggatg
     8641 taaattctcg atcgtaattt tagcggatgt ttggaaaggc atttcgaaat aggaattaaa
     8701 gccccctgta ggattaacca ctatcggcaa cgagttcacc tcgcaacgct ctccaaaccc
     8761 attacaaaag aaatccccta aaggactttc aacagcaggg ctatccgacc catcccaata
     8821 aattcttaag attaaatcac gtaaaacaaa actgccaaat tctgttcgat cacgaatcgt
     8881 catccacata tgacgaatta ttcccgttcc agtaatatcc gcaatcacag tttcttcacc
     8941 agaagtcagc ggaattgcgc cacgaccttt gcggcttggt cctagctcgc ttggcaacat
     9001 cgcagcttta cctttctcgc ctgttggatt ttcgggagtg attgctcgac tctggatttt
     9061 tttaaattgg gtaatatttt ttagtatact catctttttc tcctttgaat gtgaatcgtt
     9121 tttccctttt ctacgtgaag aatttttttc gtatcactta ttttcaatgg cttttctgac
     9181 aaatttgaaa tactcaactc ggattgcgta cattcaatcg aaatgaaggc ttgaccgaac
     9241 ttcaaattct tccaagaaaa cgttaaatta tcaacaggat caatttgcaa tactcctttt
     9301 ttgaatttca atccgaaata ttgactgatc attaggctga caaaaagacc cgatgcccag
     9361 ccgcatttgc cgcagccaaa gtctcgcacg acttctcctt gttgagcctc taagcgatac
     9421 ggccaccacc accacgaacc gtctaaatct gccaaacaaa ttagttcttc taatcgggtt
     9481 acgaaagctt gagggttgtg cagctcgctc attaaaacag tgataaagcc aggaaaagtg
     9541 gcgccggatt ctaatcccca agtgatgcct ttgatttcct cgctataggt tggattgaaa
     9601 gaactagcag aaaaggccat ggtgttctga tataaatgat ctttattatc taaaaattga
     9661 taaaaaggca tcaacgtagt gtctgattct tctccatcat gcatcaataa attaattttt
     9721 ccatccacaa tcacatcact taaaaagatt aacccttgct caagaatcgg tttttgatag
     9781 tgcttcactg aataggttgt tttctcctca tcaccgattc cctccaaaaa ttgctcacca
     9841 aaaggaccat cgatagtcat ttgtttacaa attgattcct ttagtttaca ggcaatgttt
     9901 ttgtatcgct tgctaagact cagttgttct agggcttcaa aaatttctgc aagtccaata
     9961 caggccttcc aaagacaaag attgctgccg gtatgatact ttccaagtgc aaaggcgtct
    10021 gataaccaaa cagatggaaa aagcattggt tcccctgacc gacgttgagc taatatgtcc
    10081 tcaataattc ttgaagcatt cagtagaacc tgcggacacc gctcaaagaa ttttgtatcc
    10141 ccagtatgtt caaaataaag actagccagt aacgcactgg ataacgaatt actcagagaa
    10201 tgattgatac ctcctgtaaa ttttgaacca ggaaacttaa cgccatattg atcaaaccat
    10261 agaaccgttt tttgacataa ctccggttca aacatggtga atggcaatga tgcgtaatac
    10321 atatccttat tccaaattcg attagtagca ggatagctgc cccaattgct gccgacgacc
    10381 tgtccctgtc gattcatacc gaaactagaa aagctttgat aaagtgcacg acgatagaga
    10441 tggacaactc tatcgtttgg cattgacagc tgcccaaata gctgagcaaa atagttcaat
    10501 gtttccttca gccatttttc gatatcagat tcctgaaaag tcattacttc ttcaaaagca
    10561 tttgggtcca caaaagcaat ggcgaagctt gccacttcct gttgtgcgat cgtgtcctta
    10621 tttccttttt gagaaatcaa cacattttgc tgatccgaat acttctcttg atacgaaggc
    10681 acatctttca ctgaaacttg aaggctttcg tgtgtttcat tcacaatgta aacctgttga
    10741 acaagcatac ttaaacgctt attagccagt atgggagcaa aggatactac aatgacctta
    10801 aacgtcgaat aatgatgaat atatctaggc aaacagtctt gaatcacatc gacttcaata
    10861 cgatgatccg attctgacaa atggtagaca atcccgttta cttcaacctt taaggataac
    10921 gaattggttt gagagagctg tttattgacc caaacacctg gtttttcctc agttagtaaa
    10981 ttttctcgat aaaaggtcaa cgtttgaatc gtcccgcaaa catcaaggtt tgctaatgtc
    11041 ttttgattac ttacatcgaa ggtcaatccc tttacatcag gaatattccg atagtagctg
    11101 tattcgtttg aagtagtcac atttccgtcg tttaactcag cattaaaaaa tgggtgtttg
    11161 ggtgaataaa ttacttcatt aatagtcatc ttttccctcc ttgctaaaaa aactgcagcc
    11221 tagtagctgc agcttcctac tatgcgattc caagtaatga caataaaatt cccaagacga
    11281 ccattccaaa aataattttg atgacgccga cttttttatc tagtaatttg aaacagctca
    11341 atgttaccaa taacggtaca attcctttaa aaatactatc gaggatttct tggacattca
    11401 ttagttcttc gcccttcatt gaaaagctca aaacggtatt gaatttgacc atactgatcg
    11461 tcattgcacc taccatgatc agacccaaga tactagcagc ttttgtcaga atttcaatta
    11521 acccgttcgc atacagcttt tcaatatatt tagagcctaa tgaatagcct aaaacgccgc
    11581 catagtatcg catcaagtaa ttaggtatat tgaacataac taaaaagata attggtgcca
    11641 aaatatttcc ggcattggct aagttgatcg ctagtcccgc ggtaacgaca cgccaaactc
    11701 cccagaacaa tgaatcgcca attccagcca atggtcccat caaactcgtt ttgatcgcgt
    11761 tgatactttc cacgtcaaaa ccagcttttt cagaattttc cttctccatc gatgccgcaa
    11821 tccccatgac aaaggacgcg caaggaatgg tcgtcgaaaa atactgtgta tgacgttcca
    11881 gcgccgcagc tcgatcttct tcacttttgt agaaatgatt aatgaagggc atcatagaat
    11941 aggcgaaacc accgccgccc atcgtcaccg gattcaccga agcatacata gtcatcgagc
    12001 gccaaaacat cttccgaacc aattttttct catctttaga aattccatca gctaatttca
    12061 ttgaaagaaa tcctcctctt cttggtcaag cgttgcattt acagcagcat cgctttgaac
    12121 tgcatgaaca gatatatttt ttagttgtag atctctttgg gctataacaa ttgcaataat
    12181 cgttccaact gcggcaattg caatcaatgg taactcgaga tacgccataa caacggcccc
    12241 aaagaaaaac cacacagcaa tatttttact ccaaagcatc cgtaacagca tcgccatccc
    12301 aactgccggc agcagatttc cagcgactgt taatccgttc atgatgacct ctggaatatt
    12361 atccaagacg cttttgacaa catctgctcc aaataaaacg gcaaagaatg gaataagagc
    12421 ataaagcgca tagtggacaa accacataaa gaagtgcaga ttgatgattc ctttttgatc
    12481 accagatttt gcaaatttga aaaatttagc agccatcggt ccacatacaa aagaaaaaat
    12541 catattcatt aactgtaatc ctaatactgc tagtggaaag acaatgggaa taatcgcatc
    12601 cacgtttcct cccattttga ttgcgaacgc tgtagccagc ccagcagcca aagaaggctc
    12661 cgccgtacta gagattccga tattgacgac acccataaag atggcttcta gtgacgcccc
    12721 gacaatcaac cccgtctgca aatcccccat caacgctcct gctaacggag caacaacaat
    12781 cggacgactc agcatggaca tcccgaacat ttcatgtcca gcaactccaa taaaaacggc
    12841 caaagcgact acaaatgcat catacataat ttttctcctt cctttttcta aaaatcactc
    12901 aaaatagaga tagctgaacg tttttccatc gccggtgtag gctgaaaaac ggtatctgga
    12961 taaagttcaa ctaattcttt taagtcttga atttctgacg tcgttaacat cacttccttt
    13021 gataaggttt tcttatcttc cgatggcggt gtcattttcc cagcattccc cacgttcatt
    13081 aattcgattt ccgttgggaa agctttcgca acttttaccg catctttgac attagggact
    13141 aaaaccatca agcgcatgtc cttcgcccgc ggatctttta acaaatgaat cgcgtcatca
    13201 actgttttaa taatgacttt gacattcgat ggtgcagcca tcttcaatgt catcttttga
    13261 atatcgttgg tagccgcctc atcgcttgct actactaacc cggttaaatt tagactcttt
    13321 gtccaagtca tcgctacttg tccatgaatt aatcgttcat caattcgcac tgctttaatc
    13381 atttttcttc ctcctaaaaa aaatcttcct cttcatttac tacatgtacc tcatcaagaa
    13441 tggtcatctt taattcctgt tggcatttat ttaccagttc ctgaatatcc tcctttgtta
    13501 attccttggt tgccaatact atttcgagca gaattggcag attaaagcca gacaaaatag
    13561 ttacagcaat ctctttctcc aaaaaggttt tggtaatctt ctgattgaca cttccgccaa
    13621 ataaatcggt aaaaacaaaa atctgatcct gctcgttcac ctggtttaca aatacattca
    13681 actcctgttc aaaatcacac tcatccacat aagcattaat caccgtaatc tcttgaacct
    13741 tccccgtcag taattgaata ctgctaagaa agcctgaagc taagtgaccg tgagtcgcta
    13801 ttagaatttt cttcatatgg ttaattcctt tctttctgtt cacttttata taagcaattc
    13861 ctgtgccaac ttttaaaaac attggtttaa tcacgttaat tcacattaaa aaaagtgtct
    13921 aacctaaaaa aaacagatta gacacttttt tagacacttt atttttgaat atacggtcct
    13981 aataattcat agagcagcga aagctcataa gcattgactt taaaattata tttcatctcc
    14041 attgattgaa aaatacttct agaaacataa taaaattcat cttcatctgg tttcaaatca
    14101 taattcggtt cttcagattt ttctgtccga gccaagaata aacgttccat catcaatgcc
    14161 gtatgcatat agagatttag ttttacacgg ccatttaaag tgaaatgata gtaattttca
    14221 tatttagaaa tgactgtttc cacttcactc atcacgatcg ttgggttcag aaagttcaaa
    14281 cggtccgcaa ttccttccaa cgaaaagaat tttactagct cctgaatcaa gcggttaaat
    14341 tctgccggct tcatttcagg actcaaaata tgctccaaat tggctgctcc atcaccgtct
    14401 aagatgtcat aaacattaat attaggaatt gaaaaggaac gtggaagatc cgaagtcgtg
    14461 atgaccaatt ttgtcttgtc aaaataagtt tcatcatttt ggtcgattaa ttcacgtagt
    14521 tctctgtagt ccttcgtgaa aacttcgatg gattcatccg ataaatgatg acgaaaaaca
    14581 tctttcaatt tgtccgaaat ccccaaaccc gacatacagg aaatgataat gttgctactt
    14641 tgagaaaagc cttcaaagta ttgaacattg attgaatact tgtctgctgc atcctccgca
    14701 atctgtttaa atggcgcatt catcgtcatc ttcaagccaa tatcaagtgc aatggaagtc
    14761 gtcaaattat tcagaagaag taactcccct tttaaatcgt tcttgatctg tgtgtaaatt
    14821 tgatttaaag agcccatatc tacgatcagc actagactat ctgtattcac ttgccgtttc
    14881 acgaaattcc ttgtcgattc gacaatctct gacacattac tctctactgg catatccaac
    14941 gcttcaaaaa cataattgcc gcataattga ttcactactg cttgaatgct gctagccgta
    15001 ctatctccgt gagcaagcaa caggcccttc agctcgatgg attcatctac ataatcagat
    15061 aaagtcacga caataagcag tcttagaaag ttctcgtcct tttctgagag cggtggtaaa
    15121 tgctccaaga actgattaca taagtaagtc gtccgaggta gatttgcagc taatgtacga
    15181 tttatcccgg ttaagtctgt cttatagctt ggggaaatga cataggatct gctaataatt
    15241 tctgcaacag gtccactcgt cgtcaatccg tacgtttttc ctaaaacgga tacccagacc
    15301 tttctaaaca tctgaatcat caattttcta ctcattgata aaaaagaatc cttatcaatg
    15361 tgatgcacca aatgctgtac ttcattcttt atcgaagaaa aattttcatt gaaattacct
    15421 tcatttatca gctgagtaat tttttccaaa atcgaatcat aatcaaacgg tttgattcga
    15481 tccgttttta tcttttgatt tttctttatc aataaactag caatcggcaa gctttctact
    15541 tcggattcca gcatatttaa ctgaatcaag atctcatttt tcccaatcgt taatggttct
    15601 tcttgtgttc gactatacac atccgcacaa ctgattttaa tgatattctt cagcttacca
    15661 atattcccac taaagggatg agataacatt aaagacaagg cttctcttga aacaataata
    15721 tctttttgta aaacatccgc ttcattttga aaaaataatt gaatcagctc taatcgttct
    15781 cgaatggggc gatcttcaaa agttggcaac ataacactaa tttgaatacg ccgatcaaag
    15841 gtgtctaata aataattttc acctttttcc gtcgtggcga aaataaaacg aaccttagca
    15901 gaatgccaat ttacattttc accgactgga cgatagtttc cagtatcaat aaatagaaag
    15961 agcttttctt gattttcttt cgataggcga tggacctcat cgagaaaaag atagccgttg
    16021 ttggccgttg ccaataaacc atcggtatcc ttttcagccc ccgtaaaggc ccccttcttg
    16081 tatccaaaca gtgttgccga aagcagttcg ggattgtccg cataatcggc acagtttaag
    16141 atagcaaatg gtgcatcttt ttcaataatt tgctcatgga tagcgtattc aaaaattttt
    16201 gtggctaaaa aactttttcc aacacccgtg gctccattta ttaatacact gagtccgtta
    16261 ggcggatatt tgacagcggc tatacatttt tgaatgacct ctctcagact tccgtcatga
    16321 ccaactacag aatgaaaggc ttcgtttatt tgctccgtat tatctacaac cgcatattca
    16381 tctaaaggct tccacaaaac aggctttccc ggtaatttct cgaccttttt atcttccact
    16441 aatctcgtca aataatggct ggcattctga cgagagatac ctagattctt cgaaattttc
    16501 tcggcagtga cagaagacat attccctgtt agtcgtatca gctcatcaaa aactcgatct
    16561 ttagcgctct ttttcatttt ttcctccagt ttaatgtgtc taaaagcata ataaactatc
    16621 ggctgcaata tgtctattct atatttgtat tccaattttt actttgaaac acaaatagtc
    16681 gaatatttgc ctcagatatc tgacaaaata atttatctac caatttagtt tttatctaat
    16741 atgattttta tgttttttca tagctacaaa tattgaactg gattcatata ttagttttat
    16801 taatagatta cagaagggtt aagtatattt gcatagtttc caattacttt atccttctgt
    16861 aactttttca atcttagatt tactagcccg tctcgggccc tattttacaa tggttttgtc
    16921 cacttttctc tggtagaaaa acgtggtaga aaaaataaaa taacacaaaa aaagcggcgt
    16981 ttcatccgct tttggtagaa gattggaaaa ctcagataga aaaaggacga tttttagctc
    17041 tttagaacgt tgataagtcc ccattcttac tgagttttgc cattatctcc tatacatctt
    17101 gaattctaag aataggtcat tatagatgcc taggtatttc gatcctagat ctttgtagac
    17161 ttcaagatgg gagatcgtgt catcagatgg atagaattgt tcgtccgacg aaatgtcttt
    17221 tggcaataac gcttttgctt cttcatttgg tgtagaataa ccaatatact ctgcattttg
    17281 tgccgcattt tccggttcta gcataaagtt gatgaaatca taggcacctt ctttattttt
    17341 cacggttttt ggcataacga tattatcaaa ccaaagattg gatccttctg gtgggatcac
    17401 ataatgtaaa tgttcatttt cccacatcat atcggcagct tcgccagaaa atgtcactgc
    17461 cacggaggct tcttcattga tcatatacat tttgatctca tctgccacga ttgctttgac
    17521 atttggtgtt aaatgattca atttatttgc tgcttctgtc agctgtgcca tattcttact
    17581 attcaaagaa tagcctaaac tattcaaaga aagccccata acttctcgtg caccgtcaat
    17641 cagcatcaaa ctgtctttta attccggctt ccacaaatca tcccaatgct taatttgatt
    17701 tgcatcaaag aatttatcgt tatatacgat ccctaaagtt ccccaaaaat atggaatcga
    17761 atactgatta tctggatcaa aatccaagtc taaaaatcgt ggatcaatat tatttaatcc
    17821 tttgatcttc gaatgatcta atggaagtac tagtttttct ttgatcattt tttggatcat
    17881 atattcactt ggtatagcga tgtcgtagtt tgtcccacct tgctggattt ttgtgaacat
    17941 cgcttcatta gaatcaaatg tttcataatt cactttgtat ccatattctt tttcaaattt
    18001 cgtgatcaaa ctaggatcga tataatctcc ccagttatag atggtaagta cctttgctcc
    18061 actcatgcca cttgtttttt caagttgacg aacaccaagc agcagcaccg caataatgat
    18121 gagaatacct aaaaacagag attgtaattt tttcatcgca gccgtgccac cccctcgcgt
    18181 actcttctgt ttttcttcag ccgttttgga cggttttctt tactaataaa gtagtatcca
    18241 atgaccaata tcatggagaa caagaaaact aacgcactta gcgcattgat ttctaaactg
    18301 attccttgtc ttgcacgaga atagatctct actgagagcg tcgtaaagcc atttcctgtg
    18361 acgaaaaatg taaccgcaaa atcatcaaga gaataggtaa atgccataaa atatccagct
    18421 atgattcctg gagaaagata aggcaaaatg atatttttga ctacttggaa attattagca
    18481 ccaaggtctc tagccgcatc gaccattgaa tcgttcattt cctgcaattt tggcaagacc
    18541 atcaagacga cgattggaat gctgaaagca atatgggaaa gcaatacact ctgaaaacct
    18601 aatccgaatc ctagtagtgt aaagaaaatc aagaaactag caccgataat aacatcagga
    18661 gagactaata agatgttatt catgctcaac aacgctgtcc gtgcacgtcg ttttttcgtg
    18721 taatagattc ccatagcccc aaatgtcccg atgatcgtgg cgatcaatgc agataagaaa
    18781 gccagcatga acgtattcaa tacgataatg atcaaacgtg tatcttcaaa aacagcttca
    18841 taattttccc aagtaaatcc tgtaaagctg ttcatcgttc ctccttcatt gaaggaataa
    18901 aaaatcagat agaagatcgg tgcatataag aataaaaaga cgatccctaa ataaagatac
    18961 gaccatttaa attttttcat ttatgagcga cctccttttt tcttctcgcc tgtcaggagc
    19021 atcacgatga acatagcaat gattaaaatc acaccgattg tagaacccat cccccagttt
    19081 tgtgtaacca agaaatgctg ttcgatagcc gttcctaaag tgatcacccg atttccgcca
    19141 atcagacgag tcagcatgaa taaagaaaga gaaggaataa atactgcttg gacaccactt
    19201 ttcacaccat ttaatgataa cggaaaaatc actcgtcgga aggtctcaaa gctattcgcg
    19261 cctaaatctc tactagcact gatcaaagaa ggattcatct cttccaatgc attgaaaatt
    19321 ggcataatca taaatggaat ctcgatatac gttgccacga acagaaaact gaagtctgtg
    19381 aataatatct gtttcggccc gatcccgata aaatcaaaga aatgattcac gctgccgtgc
    19441 acactgaaaa tcccaataaa tgcgtaagct ttaagcagca gattcaccca tgtaggcaaa
    19501 ataatcagca tcagccataa ttgcttgtgc ttcagttttg ttaaaaaata agcagtcggg
    19561 taactgatga tcagcgtgaa aaacgtgatc aaaaatgcat accatacaga attgatcgtc
    19621 atagaaagcg atgtccccga cgtaaaataa gatagataat tattcagcgt gaattgccca
    19681 ttcatatcaa aaaaagattg ataaatgatc attaatacag gcgcgatcac gaataaaccg
    19741 agccacaaaa tataagggac ggaatagatc ctgcgcattg ttttcattct tgtcccccct
    19801 attcttcgta gctgtcaaga cgtgcatcga attcttcttc agactcgttg aaacgcatga
    19861 catggatatc ttctggctca aaggccaatc ctactttgct tccttcttta gcttttttcg
    19921 ttgaatggac catccattca ttttgctgtt catcataaca gatgatttca taatggacac
    19981 cacgaaacag ctgagtatcg accgttacga ctagttttcc tttatctggt gtcgtgatcg
    20041 tcaaatcctc tgggcgtaaa acaacttcaa ctggttcatt tagacgcatc ccgccatcga
    20101 cacattcaaa ttgcttacca acaaactcaa ctagattatc ttcgatcatt acaccgtcca
    20161 cgatattgct ctccccaaca aaatcagcaa cgaaatgatt gattggctca tcataaatat
    20221 ctacgggtgt accactttgg acgattttcc ctttattcat gacaaaaatc tcatcactca
    20281 ttgccaacgc ttcttcttgg tcatgagtaa caaaaataaa cgtgattcct aatcgttgtt
    20341 gcagatcccg cagttcatac tgcatatctg tacgtagttt caaatcaagc gccgataacg
    20401 gttcatctaa tagcaatact ttcggttcat ttacaattgc tcgtgcaatc gctacacgct
    20461 gtctttgacc accagacatt tcactaatct cccgtttttc gtatccagga agctggacca
    20521 tacgcaaagc atcttttacc ttcttttcga tatctgattt ggacattttc ttgatcttca
    20581 atccaaaagc aacgttatca aaaacattca tatgaggaaa aagtgcataa tcttggaaga
    20641 ctgtatttac ttgtcgctta ttagctggca aatcattcaa ccgtttccct tcaaaataga
    20701 tgtccccttc tgtagcttcg gtgaatccag ctataatacg taggatcgtt gttttaccac
    20761 agccggaagg accaagcagt gtataaaatt ttccttgttc gatctcaaaa cttacttttt
    20821 ttaatactgt ttcatcatca tagcgtttca ctacttgatc aaatgaaatg attggtttcc
    20881 ccacgttttt accccctata ttacaaatat gattgcgttg caacaatcaa tacttttgtt
    20941 cgttttttga gatgattcct taattgatgt tgttttgtcg cttgataata gatcgactgt
    21001 cctgcttttg ccacatagac cgtctctcct aagcttaact ctaattcccc ttctaataca
    21061 aaaataaacg tttcagataa tgaaggttca aacgtcttat attctcctgc accttcaaaa
    21121 gtcagcaaga caggctccat ttctttttca ttcgaatcag ccacgagcca ttccaattca
    21181 tacccgtttt cttcatcgaa ataaaccgta tgatcttctt ttgaataaac cacttgcaga
    21241 ttcgctgctt cttgatgaaa aaattcttct ggagtcacac ctaagacttc aagaatagaa
    21301 aagaatgttt ccattgatgg tgaacttaaa tctcgttcca actgggaaat ataccctttt
    21361 gacaaatctg tgcgttcgcc tagttcttct tgtgtcaaat tcttttggat gcgcaaattt
    21421 cttaacttct caccaatttc cataatttcc tccgaatcga agttttctaa tagtaaactt
    21481 cctgtttact accactcgta ttagtatacg aaatttattg aaaaaggcaa ccctttgtaa
    21541 aatttttttt gctgaatatt tctttatctt ttggaataaa ttctagttgt ttgattcttt
    21601 atttttcacc taatttttgt ctaaatttac ggaagaacaa gaataaaaga gtaggcttta
    21661 tggtatgata aaagaaaatt ttaaaaggag tgtctcacga atgaacgctt ttcgatcttt
    21721 tcaggtaata aaggaacctg ttttccatac agcaatcagt gatctgccat ttactgagtt
    21781 ggctgaaaat gaagtcttgg taaaaattgc ctattctgat gtcaattata aagatgcatt
    21841 ggcaatgagc gagtctggtc aggtgatacg aacatatccg atgactccag ggattgattt
    21901 atccggaact gtcgtccaaa gtaacaatcc acgtttttca aaagaagatc ttgtacttgc
    21961 tactggtttt gggcttggcg tcacccatcc aggcggttat agccaatatc aaaaagtacc
    22021 aggagactgg ttagttcctc taccaaaaaa tatgacgctg cgtcaagcga tgatccttgg
    22081 tacagcaggg tttactgcta tgttgtgtgt gaacgcttta atccgtcaag gtatgacctc
    22141 cgataaaaaa gttgtcgtta ctggtgcttc tggcggtgta ggcagtacag cgatagccct
    22201 gttacataaa ctaggattcc agtcaatcat tgccttttcc agaaaagaag agtctgtcac
    22261 atggctaaaa agtctaggtg ctttgcaagt cgtacgacct gatgaatttc tgccagagac
    22321 aacaaaagct ttaggaaaac agcaaatcga ctatgtcatc gacacagtag gtggcgagca
    22381 gctaagcagt cttcttccat tgatctctta taacggtgca gtagctttat gtggcaatgc
    22441 tgggggaatc aaactaaacg cgactgtctt gccttttatt ctgcgaaaca tccagttgat
    22501 tggaattgat tctgtcaatg taccaataga tcaacgtctt tctttatggc aacagatggc
    22561 ggatttgcag attgcagatg aactggtcgt ccaagagatt actctggatc aattgcctga
    22621 aacagcatct aaactacttg ctggaacaca ccaaggaaga acactagtaa atgtaggtga
    22681 ccataaatga agattctaat cacagctgga ggaacgactg aaaaaatcga tcaagtacgt
    22741 gctattacaa atcattcgac aggacgatta gggcaagcat tagctgatta tttagctgcc
    22801 aatccggata ccacaatcga ttacgtgact acgaaacaag cactgaaacc gaaaagacac
    22861 tccaatataa cgatttatac gatcgaatct gcactggact tatttttaca attagaagaa
    22921 ctaacgaaaa aagaacacta cgatgcgatt atccacagta tggcagtcag tgattttact
    22981 cctgctttta gtttttcaga agaacagctg gcaaaaaacc tacccacttc ttcaacgcaa
    23041 gaagaactgg acaattggtt tgcggaaaat gaacagacag ataccactgt ttcaaagatc
    23101 tcttctaaca ccgagcatct tgtgctcgtt ttgaagaaaa caccaaaaat catttcttat
    23161 ctacgtgaat ggcagccaaa agcaaaaatc attggtttca aactattagt agatgtacca
    23221 aaggaatctc ttctggcagt cgcaaaaaat agtctgataa ataacaaaac tgattttatt
    23281 ttcgcaaatg atttgacgga aatccatggc gagacacacc acggctatct cttatctaaa
    23341 gatggaacag tggaagaagc tcaaagtaaa tctgagattg ctgcattgat tactgagaaa
    23401 attagactgg aggaatcaaa atgaaaaaga tcttactagg cgttactgga agtatttctg
    23461 cttacaaaag cgctgatctt acaagtcaat tcgtcaaatt aggtgcccag gtagatgtga
    23521 ttatgtctaa aagcagcaca cattttatca cgccattaac cttgcaatct ctgtcgaagc
    23581 gcgcagtcca tacagacgtc atgcaagaat caaatccgca ggtaatcaat catatcgaac
    23641 tagcaaaaca agctgacttg tttctcatcg cgcctgctac agcaaacatt atcggcaagc
    23701 tagcaaacgg cattgcagac gatcttcttt caacagtcgc aatggcgctt ccagtgggaa
    23761 caccaaaatt gattgcccct gccatgaaca cgaatatgta tcaaaacccg atcactcagc
    23821 gtaatctgga aacattgaga tctgtcggct atcaggaaat tgaaccaaga gaatctcttt
    23881 tagcttgtgg agattttgga aaaggtgcct tagctgatat tcagacgatc atcgaacagg
    23941 tcatgctgac tcttaatgaa agaaatgctt agaaaaaagg agtacttatg aaaaatacaa
    24001 aaaactttac attaactgcg atgtttttag ctattttgat tttattagct gttacgcctt
    24061 taggttttat ccctatcggt ccaatcaatg caacaaccat gcatattcct gtaatcattg
    24121 cttcgatcgt tttaggacca agactaggtg cttttcttgg cggtacattt ggactgatca
    24181 gcatgatccg tagcacagtt atccaaacac ctttgtcttt tgtcttttct ccgtttatcc
    24241 cagtcatcgg aacagaacat ggaagctgga aagccttgct gatcgcgttt attcctcgca
    24301 tcttgattgg ggtggttcct tatttcgttt ataaaggtct gcgcaaacta atgaaacaaa
    24361 aagcagattc cgtttcttta ttcttagctg gactttccgg ttcattgacc aatacgattt
    24421 tagtgatgaa tatgatctac tttttattcc agcaagatta tgccaaagtc atcggaacaa
    24481 atttaaatgc tgtttattca gctattctag cggttatttt tacaagtggt atacctgaag
    24541 gaatcgtcgc aggtcttgcg actgcagcag tatgcaatgt cttgttaaga tacttcaagc
    24601 acccttctct tactaactga tagtcatcgt agccgatctc tgattttttt ctcttttggt
    24661 gttactattg aatggaaaga tttgacgtcg aaaggatgat ccaaatgaaa atttttgttg
    24721 taggtgcaaa cggacaaatt ggccgacacc tgataaaaga tttagctcca agttcccatg
    24781 aaatttttgc aggggtgaga gatgttgctt cccaaacatt agtaaaaaaa gagaatgttt
    24841 cgtatgtctc ttttgattta acttggtccg tggaaaaaat gaccgaagca ttcaaaggca
    24901 tcgatgtatt gatttttgct gccggctctc aaggaaagaa tcttctgcaa gtagacttgg
    24961 atggcgcaat caaaacagtg atcgcagctg aaaatgcgca tgtctctcgc tatctgatgg
    25021 tcagtgctgt ttatgcagat gaaccagcaa aatggcctga gtccatgaca gattattata
    25081 ttactaaaca ttatgcagat gaatggctga aacgaactaa cttagatttt gtcattcttc
    25141 aaccagtcac attgactaac gatgaagaag ttacttccat tcaattaact aagcctaatg
    25201 agaaggcatc aaaaacgatc actcgctcta cagttgctgc tgtacttgcc gctctagtgg
    25261 aagaaacgga tatttctcgt acgactctag tactttctga aggtagcaaa gagttgaata
    25321 cagcattcca agaatgggca aaggaggaat agatccatgc gtgccatttc aatcaagcat
    25381 cctggttctg tagatgtatt agaacaaatt gaagtgcctg ttcctaaacc aaaacaaggc
    25441 gaattactga tcaaagtcca tacagcagct gtcaatcgaa cagatattat gaaaagagaa
    25501 tcagtctctt tgaaacctcc ctaccctatt ttgggagtcg aagtagctgg agaggtagtt
    25561 gagaataaaa gtgatatctc cacttttact cctgggacaa aagtatacgg attagtcaat
    25621 ctaggtggtt atgccgaata tgctgtgatg cctgccgatc gtgcaatcct tctccctgat
    25681 acgctagact atgtttctgc tgctggaatc tcagaagttt ttttgactgc ttatcaaaca
    25741 ctttactagc ttggaaaatt acaagaaggc gaaactgtct tgatccatgc aggagcaagt
    25801 ggtgtgggaa cagcagcgat ccaattagct agacagcttt ctcacgcgaa aatcatcgtt
    25861 actgcaggat cagaagaaaa actcgccttt tgtaaagaac ttggtgcaga tgaattgatc
    25921 aactataaaa aacaagactt tcaagaagag gtacgaaaaa tcactgaagg tcggggagtc
    25981 gatgttattt tggatttcat cggcgcttct tactgggaaa agaatttagc ttctatagcc
    26041 gttgatggac gttgggtctt gattggtatg ctaggtggga ccatcgtgcc tgagatagac
    26101 cttggtgtat tgattgggaa acgtgtccaa ctgcttggta cattattgac acctagaagt
    26161 gatcagtata aagcagagct cgcacaaact tttatgcaaa aagctgggcc ttttctagaa
    26221 aatggcaaga ttcggccaat cattgaccgt acattccaat tcagcgcagt caaacaagcg
    26281 catgaatata tggaagcaaa caaaaacatc ggaaaaatca ttttgaagat aactgaataa
    26341 aatcatgctg atcaaaaaag aggctgggac agaagcgttt aactccaaga aataagaaga
    26401 aattcacgaa aattgctttt caaatttttg tgaatttcag cttatttccg aaggaatttc
    26461 ttctgcttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt
    26521 cccacgctct ttttccgaat aaacggtgtt caaaagcttg catctgcggt aataagtcca
    26581 accaagttaa aaacataaga agctgttttt acttggctgt tcttattact tgatgcaggg
    26641 cagctttttc acaacctctt cactaaacag tgttcaatca gctgaccctc ggtattagct
    26701 caaaacgctg atttcacaac ctctttttat ttacgttcta tttcattcat tttccgaagc
    26761 aaatcgagct gcttgtatgc ccgcttgtcg cccaaaaata atgatctccg caactgaatt
    26821 tccaccgatc cggttgtttc catgtaagcc acctgtgact tcaccagcag catataaccc
    26881 cctaattggc tggttttctt tatctaatac ttcagtgttc gtattgattt tcacaccgcc
    26941 catcgtgtaa tggattcctg gtgcgatttt gatcgcataa taattcggag acgataagtc
    27001 atgttccatg gctgtggcac ggttgaattg agcatctttt ttctccgcaa catccgtatt
    27061 ccatccatca actgtttttg ctagtgtttc ttttggtaaa tcgatttttt ctgctaattc
    27121 ttccattgtc tttccagttg tgacatatcc tttttggtca taaaattcaa tagctgtggc
    27181 acgatcacgc acgccttggt caaaaatcaa ataagctgat ttttcaggca atgctgtaat
    27241 tgctgcagat actttatccc ttgtatccat ttcattgaca aatcttttcc cctcttgatc
    27301 gaccaagatt gctccttctc cgcgaacaac ctcaccaatc aagaccccct catcctgctg
    27361 cacagttgga tgaatttgta ttttatccat atcaacggtt tgtccaccta atttttcaat
    27421 catggtgata ccatctccag ttgatccttc ttgatttgtc gtaatataat cagctaaatc
    27481 gggacgatat ttttcaataa attctttgct ggcaccaaaa ccgcctgtgg taactacgat
    27541 tgcttttccg gtgacctctt ttggttcttc tccttgaaca tgtacagtca cttcattgac
    27601 cgtttcccca tttttcttaa tgtctgtcac atctgcgttg acaaaaatag ggatttcccg
    27661 ctcatggacg ttattcaata acccctccac taagtatcca ccaatagccg agccatcaga
    27721 cggacgatgt gtcctctttt cactcatccc tccagtgatc gttaagttgt ccagtttgat
    27781 accgttttga tctagccaat cgaccgcatc agcagagtga tcgacaaagt atcgaagcat
    27841 ttctttatca ttcgttcctc caccaccttt tagcgtttct tcatagaagg catcattcga
    27901 gtctttgatt ccttcttctt tttgaaattt tgtttctgaa gcattcatac cgcttgatga
    27961 tttagatgtg tttcctccgg caacaggcat cttttccaaa attactggat tcaacccagc
    28021 atcttttgct tcgatagctg ctgccatccc tgcaccacca gctccaataa tcacgacatc
    28081 ataagattct ttcatttctt ttggatctgt atatacttgt tcagaagcgc ctgatgatac
    28141 ttcggcagat tccttcgtac tagaacttgt ctgcgctgta tttttctgat ctgcgccgca
    28201 tgcgctcata gcaagaagtg caacggctgt taataaacca gcaactaatt ttttcatttg
    28261 tatccccctt ttctccttat actatattaa gtgatttttt tcacaatatc aactctgttt
    28321 ttctttaagt catatagctt tggtaaacac acgcttctta gtataattaa ggatatacat
    28381 gaaggagtta ttttatgaat catttgaaaa ccttttttac gaacagtaca aaaactgcag
    28441 gtagaatcac aaacggaatc tatattttat tacgtatcat cgtcgttatt atgctagtgg
    28501 atcaggtctg gaaccaacac tggggaaata caatgctttt attactgaca ttaattttat
    28561 ttgaaatccc ttattttctg gaaaagatat taaagataca gattccaaat atgctagaaa
    28621 tcattttgtt atcttttatt tttagttcaa cagttttagg agagttaagt gatttttatg
    28681 gctatttttc aatgtgggat acttttctac atgcattcag cggattttta gctgggggtg
    28741 tcggcttttc ccttgtttat ttgctcaata aaaatacaaa aaccatgaac ttgactcctt
    28801 tattactagc agttgtttcc ttctgttttt cgatgacagt tggtgttatg tgggagtttg
    28861 tggaattttc agcagataaa ttactgcaat tcgatatgca aaaagatacg atcatccagc
    28921 aaatcaatag tgtggagtta agtaaagacg gaaacactgt ctatcatgtt ccagatatca
    28981 ctcggacaat cattgaaagt caaaatgcag atggtaaaac agataaaaca atcatccaaa
    29041 atggttactt agacgtggga atcatggata ctatgaagga tttatttgtt aattttattg
    29101 gagcagttgt tttcagtgta tttggttacc tctatagtcg aaacgattac caaaaattta
    29161 cgttcatcaa gaattttatt cctaaaaaaa tcaaacgata aaacatgcta aaaaacaaga
    29221 gaaggggcag tgacaatact tttgtcactg ccccttcttt tttgaatgta tggcatgcca
    29281 ttagttatcc gatagtatta actcaaaaaa gtaagatatg aggaatgact atttcgtttt
    29341 ttatcccaat actgaggact tatttcacca tttcgttatt tatctaattt acttaaaacc
    29401 tgcataactt tttcataatc tggattaggt gtgccgtcta gattttcagc aataaaagta
    29461 aaacccattt ttttataaag agcaatcgcc tgatggctcc atgtctgtgt gtgaagatag
    29521 attggtccgt ccgtatggag ggtaggaaat tcttgcaaga ctctggcagt caatgctttt
    29581 gcgagccctt ttccttggta atctggtttc actgctaacc aatgaaaaac cggatacact
    29641 tgctctcttg atttttttcg ccaagcggta caggttgcta ctttttctcc tttttttgtc
    29701 tctacaaaaa acatccttct ttcaagttct tcaggataag gagcaaagtt ttgttgaaaa
    29761 tactcaagtg cttcatctgt cgtttgaaat tccccaacgg aagtctcaat ctccgcccaa
    29821 gcagattcat cttctttttg ataaaatcga aattcaaaac cttccgctaa aggcgtgatt
    29881 ggaacatttt gttgaagtgg tcttgtcatc cagatttctg cgtaaggtaa cgttttatct
    29941 aacatttttc tatccctcct cgtctccatt atgccaaata acatatgact cagctactta
    30001 tttgactata acagatagag atataaatca gaaccagtca tctgcgtcaa attcatttcg
    30061 attagcgtat acccatgttc ctgccatacc tcgcatgaaa tctcctaaag cagaagaagg
    30121 aatacctcca ccaggtgcgg gtgttcccgg actaggaagt ggtacttgcg gctgcagatg
    30181 ctcatcggtg gaatcaatag ttgacaagcg ttttttcttc gtttccttct ggtcatctga
    30241 ttccttctga tcttccgact gtttagcaac aaattctttt tctgattgtt gttttatttt
    30301 aggctctaaa atttcatttt ccaatccaga aacttgtggg gccaaatgag cttcttgcct
    30361 tatcgtttta ttagattctg agatttttct agatattctt gctaactcta ggcttcttgt
    30421 ttgttttact ttccctgttg gagaaactgc atgagccatt tgttgttttt tttctgacaa
    30481 gctttcttct tgtctttttt gttctttttg atctatctgt ttgtttaatg gtaaaacatg
    30541 taatagtgac tgagcatgga gttcttggag tgaatacggg tcatgataac tatacataat
    30601 cgtaaaatca cctgtttgtt tggtcaaata accatcctcc aatggtactt cattgattgt
    30661 aaccgataca tcctttaacg gtacgggttt acctaaggaa tttgttgctg attggatcaa
    30721 ctcttctttg tttatttttt cgtttggatg aacaataaga tcatgacctt ctaatgaacg
    30781 attgtctggt atataaacat aagtaaaggt aatttcccct cctgattgct tcctattatt
    30841 catcagttct gagtgaagta cttcaaggat ctccgaccag tgactttttc tgtctaatcc
    30901 gtttgcttgc atccaatcag aaatagaagt gactttccca ttttgttcat aagtcgataa
    30961 atctatattt ggcactcgat cagaaaattc agcaggtagg cgcaaatcag gaatacgatt
    31021 tacactactc atatgtgttt ctgcttgaag ttctttattt gtttgactat ctataaaatg
    31081 agtgatgaca gtcgatgaag cagcagatgc ttctacataa actacagctg ttgtttctgc
    31141 cacatttgat gttttcgagt ctatataagt taatacaacg ggatattctc ccggagttgt
    31201 agtatctact gtcccgttca ttttgatttc ttctttattt cctgaagttc catcgctttt
    31261 tgttacatat tcaacaagat ctttaggggc gtaactctct cctgttctta agtgaagatc
    31321 ttttcctttg atagctgttt gatcagcacg aacatggatc acagcagatg cagtcattgt
    31381 ttttaaagca attggatcaa aataggacaa ggaaatagta gatgttcctg tattagtcgt
    31441 atccacgata ttcccattgt ttgaaattac tttggacata tctccttgtg ttccatctgc
    31501 gttcttcagc ctttctacca gatcagatat cgaaactttt gtccctgcca gaacatcgat
    31561 tttctttctt tcaaactgtg aataatcttt ttcatataca taagaaactt gtgcgcttcc
    31621 accgtccttt atatctgtca aagcatgatt caaaatatca gtgatctgtt ttaactgatc
    31681 accttttgta ggatcagtca tatcggaaaa gatagaaagc ggcacactgc ttccggtttc
    31741 cgatgtccat aatgttttgt taggaattaa ccagtagtgg tttatcgttg gaggataact
    31801 gactttatta taggaagtca atgcaggaag tagctgtttt ccatttgcat cagtgaaagt
    31861 atgagtaata gtgatagggg aatcagtggt ctcagaaaca gaggtacgta cgaccttgat
    31921 ggtttgtgtc gttgcctgaa cgggtacatg catatagaca cctacagata aactgatcag
    31981 catcgtgcgc atcataaact taacgtagtg tttttcagaa cacgaccgtt ccttatctaa
    32041 acgacaagcg tgtttattta gttgttttga acgtcttttt gaacacattt attcttagtc
    32101 ctccctactc ttgtctaaac atgaagtagt aattttcata agatgatttc attatataaa
    32161 taaaatcaaa agaagatgga ctattaggag agtatttttt ttgatttttt attgtttcaa
    32221 gaaaatcgga ataataactt atgtactttt taattttgtt aagtaatata tctagttata
    32281 tctagttata tctagttacc ttttctttga aaaaaacata aatcagctaa taaatatgtg
    32341 agacaaagct gccatccata aatactagct gattttttca gttaaatgtc tatttttttc
    32401 tctgatgatg gtatttccaa aatattccca gtcatctgga gtcggtggat ttttccaaat
    32461 tatctgttct ttattaaatt tttcaagtaa acaatcagat aaataaatat tcgtacgaga
    32521 agtgattcga ctctcaatca caatattgta atttttaaat cctttcactt gagaaataat
    32581 gtaatcggta taagcagatc cttgcgaaaa atcaatacag acatgaatcg gtgcttcaaa
    32641 atatgatacc ggacaaatac cgaccaataa aaagagataa tcataaaata agcgaatcat
    32701 gaaacgttca tcttttccca agaaatagcg tttattctct tgtatgacct tttggattgc
    32761 tgtactgaat attggatacg tctcgaagaa aaactgtagc tgctttttag acgtaaagct
    32821 agtcatctca aagtcgaata tccaattttt tcgattgaca tgcatcatac ccatacgaag
    32881 ttcttttcgt atatatagtc tttcttcttc agagatttca atcgaaaatg gtaattgatc
    32941 aatcaattcc actgacattt tttgactaat catgtggata tcatgaaaat gagcttccgc
    33001 aagtccaaat gcgccatcta attcaaattc ttcttccgtg atcaaaaaca acatgatata
    33061 agaaatctca tcaatgatct gctctttagg gatatgaaga aaagaaatat gtgataaatc
    33121 cttgatgatc ttcattcctt ctgagctctt aaagtattca tctgaaaaga aacgttttcc
    33181 gattggttga ttcccatagg ccctcaacaa actaatagtg gttagcaaat tgaatttgat
    33241 tcgttttgtt gcagacagtt ttaaattgaa cagatattca aaatattgca cgatactctt
    33301 tgcttcattc atgacgcttt ctctaaatgg catagaaaaa ccattaaaga ccccgaaata
    33361 taccccaaat aatacgttcc gtatgtttat ttcttcccca actaatatgt ttttctttaa
    33421 ctttatttga tgctgttcaa gaaaagagac taattctctt ctttttacat aggctcttga
    33481 tatgcttaaa aaatgattct ctgcaaattt ttcgatcgtt acttttgaat ccacgatctc
    33541 cgttaacagt cgagcaattt ttgatcgttg aaagtagatt cttcttaaat ttttgatatc
    33601 cgtcaaattc agacgttcga catggatctc tgcttcttct gaaatttgga tctctggctt
    33661 tgttttgaat tcagtaaatt ctttatttaa ttctatgata taatttcttg ttttaaattt
    33721 agacaaacct aatgtttcat ataacttagg cagcactaca taattctttt tctcaaattc
    33781 taaatatcta gctagcagca gtttttctct atcttcctct tctataaggt aatccaactt
    33841 catatcctac acctcaaaaa atgcctcata ccaattactt gatatgcaga tgaaagttca
    33901 tcttttcacc tatactgatt ttatgatgga ttatgcttgt tttttcatca gttattctca
    33961 ttttttatct tttttttgag aatattgcaa aaaatggaaa aatttatgag gaaatattct
    34021 atattttttg ttttattgtg ttaaaatgta caagatttgc gccatttgcg taaacaacca
    34081 caaaaaagag caggacggcc accgtcctgc tcttttactt attttaagtt ccattactta
    34141 ttaattggct aaacttctgc ttgtttctga tattgcttgt ttttctccaa tgatccgcac
    34201 ttctgtttct aaagacacat caaatttttc tttgattact tcttggatgt gcgcaatcaa
    34261 ttcaatataa tctgtcgctg tcgcatgatc gatattgacg atgaatccag catgtttttc
    34321 agagacttgt gcaccgcccc atttcaggcc ttgcaatcct gcttgttgga tcagctgacc
    34381 tgtatagtgt cctactgggc gcttaaagac acttccgcat gaagggtatt ctaatggctg
    34441 ttttgattga cgcaattcgg tcaactcatc cattcttgct ttgattgctt cataatcccc
    34501 tgtttgcaaa ctgaatcgag cttccaatac gatagcacgt aattcttgga ctttactgtg
    34561 acgataagaa aataccattt cgtcttttgt caaagttttc aatgttccat cttcaagcaa
    34621 aagatccact tcagcaaaaa catctttgat ctctccgcca tacgcacctg cattcatata
    34681 aaccgctcca ccgacacttc ccgggatgcc gcaagcaaat tcaaatccag tcaatgattc
    34741 tgccaaagct gcataagtcg tatcaatcag tgaggcacct gcttccgcaa tgaccatcgt
    34801 tcctttgaca tgaatctgtt tcatttcagt cagcatgatg actacaccgc gaatcccgcc
    34861 atcttgaacg atcagattgc ttgcatttcc taataccatc caaggtatat catggattcg
    34921 gcaataatcg atgatttgct ttacttcttc ttgttttttc gggaatgcaa ggacatcagc
    34981 tggtccacct gttttcgtaa acgtatagtt catcaatggt tcatcaaaca atatagtaat
    35041 cgtggctaag ctttcaacta tttcttcttt attcactgag gattttcctt tcttttctca
    35101 aacgtcctca ttattctatc acgcttaata caatcttgcc agtcttgttc aaagttgatg
    35161 acggtagtga tctttaagca cttcctcaca taattcatga cttcttacaa cttctttttg
    35221 tttaagcaat tctgtttccg atttacctaa acggatctga gaaaagaagg cctctaccat
    35281 tggctcaaac ccgcgtttga ctagcgtgtt tgtccagtcg ccaaattctt tcactttttt
    35341 tccatcagca tcttgaatga caagctgggt cagatcgttt acttcataag taccattttt
    35401 acttgttact tgatattttt ctatattagc gccacttgct aaatccatcg ttaaaatagc
    35461 cgtttgattt tgtgtttcta attgtaaaat ggcatattcc aaaaattcct ttgtttctct
    35521 tatctggtat tttgttctca aaatgggttc atctaataaa tacacagctg tatcaataag
    35581 atgtaaaaac aaatcataaa ggacgaactc tgtcgtttct tttgccgcta ttcgattttt
    35641 ttgcaggtgg atcaaccgct tatctgggat acgtttcaat tcttctacca taggagcaaa
    35701 gcgccggttg aaaccgatca tcaaaattat ttggttctta tcagctaatt cttgcaattc
    35761 acgaacttca tttagatgga cactcagtgg tttgtcaata taaacatgga taccattttc
    35821 tagacatttt ttggctaatt cataatgtac ttttgttgct gcgtggatca tacatgcttc
    35881 gattttttct tctaaaagct catcgatcgt ttcatataga tgttggaacc cgtactcttt
    35941 ttgaattctt tcttttgttt ctttatttct tgttgcaaaa taaaaatcaa gtgttccttg
    36001 ttttttcgca taagttggta aatatgcttt ttctgcgata ttccctacac cgattacacc
    36061 aatcttcatc tactttcctc ctggatcatt tagttacttt catcttacac cattttttac
    36121 taaaatgagg tacaatagta tcgcaaaggt aaggaaatta attcgaagga gtgtgctttt
    36181 attgaaattt atctcttgga acgtaaacgg gcttcgtgcc atcgtaaata aaaatttttt
    36241 agaagttttt catgagttag acgccgattt tttctgtctg caggaaacga aactccaagc
    36301 aggacaaatt gatttagatc ttccaggtta ttatcaatat tggaattacg cggaacgaaa
    36361 aggctattcc ggcacagcga tttttgctaa aaaaccagca ttgaatgcca cttacggaat
    36421 gggtatcgat atccatgata cagaaggccg tctgattact ttggaatatt ccgatttctt
    36481 tcttgtaacc tgttacacgc caaattctca aagcgaatta aaacgtttag attaccgatt
    36541 agaatgggaa gaagcctttt ataattattt ggaaaatcta aaaaaacaaa aaccagtcat
    36601 cgtgtgtggg gatctaaatg tcgctcatca aaagattgat ttgaaaaact ggaaaacgaa
    36661 tcaaaaaaat gctggattca ctccagaaga aagagcggct ctttctcgac tgttggataa
    36721 cgggtttatc gatacattcc gttattttta tccaacccag gaaggtgtct actcttggtg
    36781 gaactaccga ttcaattcaa ggaaaaacaa tgctggttgg cggatcgatt actttttgac
    36841 gagcaaagat ttagagccag gactagctga tgcaaagatc catacggaca ttatgggcag
    36901 tgatcattgt ccagtagaat tagatttgaa ataaagaggt tgtgaaaaag tcgtttagtt
    36961 tcgagtatta agaaaaattt tgtgaaaatg gctttccaca ttttgcataa atttggcata
    37021 ataccgagaa actggctttt gaacactgtt tatcgagaga ttgtgaaaaa gctgtcctgc
    37081 atcaagtaat aagaacagcc aaacaaaaat agttcctcat atttttcgtt tggttgggct
    37141 tattaccgca gatacaagct tttgaacacc gtttattaag aggttgtgaa aaagctgcac
    37201 tgcatcaagt aataagaaca gccaaacaaa aatagttcct catatttttc gtttggttgg
    37261 gcttattacc gcagatgcaa gcttttgaac accgtttatt aagaggttgt gaaaaagctg
    37321 cactgcatca agtaataaga acagccaaac aaaaatagtt cctcatattt ttcgtttggt
    37381 tggacttatt accgcagatg caagcttttg aacaccgttt attgagaagt tgtgaaatca
    37441 gcgtcctgac ctgagtatta gagaaacaaa tggcaaaata gttcctcata ttttgctatt
    37501 ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tagtggagaa gttgtgaaaa
    37561 agtcgcctac tttcgagtcg taagaaattt ttttgaaaat ggttcctcac aactttgcat
    37621 aaatttgact taataccgag aagtcatgat ccccatctaa ctccaaatca caaggaatga
    37681 gttcaaaaac tagcttttct caacaaatat tacataaaaa attctatgtt ttttgttaaa
    37741 tttatttgct tttttcaaaa gtatgttaca gtaatttccc aaggaggttt tacagaaatg
    37801 aattttgttg ccatggattt tgaaactgcc aatcatcagc cttatagtgc ttgttctctt
    37861 gcgctagtca tggtaaaaaa tagccagatc gttgatgaat tctatacatt gatacagcct
    37921 gagactcctt ttttctggcg aaatgtccaa attcacggaa tccatcaaga agacgtacgc
    37981 aatgcgccga aattcccaga agtctggcaa actatccaac catattttca aaaaaatcgt
    38041 ttagtcgttg cacataatgc tgcttttgat acaaaggtct tagcgggatg cttagattat
    38101 tatcatcttg aacagcctaa ttatctttct ttatgtacag tgaaaagtag ccgtcgcctg
    38161 tttcctgaat ttccaaatca tcgattgaac actgtgtgcg aaaatttgaa catccaatta
    38221 gatcatcacc atgatgcatt ggaagatagt cgtgcatgtg cggaaatcct gctttatcaa
    38281 gaaaaaaaat tcgggacaga tccattgaaa aaacttgtga ctataaagta aaagaatact
    38341 tatgaaacac acaaagaggt cgtgagcctg ccgtttcact ccgagtcaca aggagcaatt
    38401 tcaaaaaata gcttctcatg tttattaaat attgtgactg gtgaccgagg agctggcagg
    38461 agaacaccgt ttattggaga ggttgtgaaa aagctgttct gcatcaagta ataagaacag
    38521 ccaaacaaaa atagcttctc atatttttca tttggttgga cttattaccg cagatgcaag
    38581 cttttggaca ccgtttacca agaggttgtg aaatcagcgt tttgacctga gtattagaga
    38641 aacaaacggc aaaatagttc ctcatatttt gccgtttttt gagctaatac cgaaggtcag
    38701 ctgattgaac accgtttatt caaaaaaaat ggactgcgac acgacttttg tcacagtccc
    38761 ttcgtgtgtt tttctgcctt ctcaaaagga tgacagtttc tcactctttt cctatttctt
    38821 ctattcaatc aaataactca ttttatatac atctaaaaat tcacctagat ctgttcttgc
    38881 ccctctaggc atgaccgctt cgatttggaa gcccattttt cggtaaaggt gtacggctct
    38941 ttcattacga acttgaacgt caagctccaa tcgaaaaagt acacccattt ccttcgccca
    39001 actgatgatt tcttctagca taagagtgcc tagtcccatt ccccaatatt cctgcagaat
    39061 actgataccg acttctccca cgtgagacaa acgaaattga ctgtctgcct tgacagatgc
    39121 tgttccaata atcgtccctt catagattgc aagcagcaat aaattattat tgctttcgta
    39181 tatataatcc aactcttctt ctaaggtttc tggtggtaac aggagttctg cttcatccaa
    39241 aactaaaaat tctgtctcct tattaacttg tcccatcatc aaaagaatgt cttttgcatc
    39301 tcctggcact gcttcccgca agatgatccc aacttcttca ctcatgattt tcagcccatt
    39361 tttctagaat tcgctgtttg aaggtttcac tttgctcccc gtatgagatt agtttcacat
    39421 accgcttttc taaatctgta tcactttttt ctaaaatcag ttccaaataa tcctccggca
    39481 gtgcttcttc taatagattt ccccattcaa caacggaaag cccatcacct tcaaaatatt
    39541 catctaaccc taaatctgct ccgctagctg ctacccgata aatatccata tgatacaacg
    39601 gaatccgacc ttgactatat tctctaataa tcgtgtatgt cggacttttg atcatttgcg
    39661 ttatccctaa tccttgggca attcctttcg tcaacgttgt tttacccgca cctagatcac
    39721 cagtcaatat cagattgtca cctggcattg cggaatgacc aattgcctct gcgaattgtt
    39781 ctgtcattgt taatcctgat agttcgatca tttttcatct tctcccgctc ttctttcttt
    39841 ttgccagtat atcgcgtaat gaagtttttt taaagaaaaa atgaactagt caagaacagc
    39901 agctgctgtt tttggctagt tcgcgtgtat ttgttaatca atcaatgttt gtgctgctgt
    39961 gataatcgat aatttatata catcttcttc atttgctcca cgagacaaat cagaaaccgg
    40021 tttgttcaag ccttgtagaa ttggtccgat tgcttcaaag ttaccgaaac gctgagcaat
    40081 tttatagccg atatttcctg actgcaattc tggaaataca aagactgttg cttgaccagc
    40141 tacttctgaa tctggcgctt tcagttgtgc aactgaagga acataagaag catcaaattg
    40201 caattcgccg tcgatcatgt attgtggcgc tagtttttta gcgattttag ttgcttcaac
    40261 tactttgtct acttctggcg atttcgctga gccttttgtc gagaagctca tcattgctac
    40321 ttttggttcg atatcgaaca attctgctgt ttttgcagaa tctacggcga tttcagccaa
    40381 ttcttgcgca gttggattca cgttgatcgc acaatccgaa aagacatatt tttcttgatc
    40441 tcttccacgt accatgatca tcgctccgct tgtccgactg atgccaggct ttgttttgat
    40501 gatctgaagt gctgggcgaa cagtgtcgcc agtcgaatgg atcgctccag agaccattcc
    40561 gtctgcaatc cccatatacg taagcatcgt accaaagtaa ttcacatctt ttaaaatctt
    40621 ttctgcttgt tctttggatg cctttccatt tctacgttca acaaaagtat ccaccatttc
    40681 atcccactta tcgtaattat cagggctgat gatcgtaaag ctagatgttt tgattcctct
    40741 ggcatgcgct gcatttctta tttcttcttc gttccctact aaaatgggtt ccacaagttc
    40801 ttctgctttc aaccgggcag ctgcgcctaa aatacgagca tcagtagctt ccgggaaaac
    40861 gattttgatc ccacggcgaa caattttaaa tcttaaacta tcaaacaatt ccacaagatg
    40921 acctccagac gtttttatat gtattatcat acactattcc agaaaaaaag aacagtttat
    40981 tttatctgta tcagtcaaaa atgctggaaa aattaagttt ttcacataca aagcgaactg
    41041 cactataaca ggaaagcgct gttttattac actgtttcag gcagttgcca atcaatcggt
    41101 tcttctccta acgcaaggag cgcatcattc gttttcgaga agggtttgga accaaaaaag
    41161 ccacgacttg cagataacgg actgggatgt ggagatgtta tgatgatatg gcgactctta
    41221 tcgatcattt tgattttttc ttgtgccggt tttccccaca agataaacac gattggtttt
    41281 tcccgttcat ttaatttttc aatgatcgtg tctgttaatc tttcccaacc tttcccgcga
    41341 tgagaatacg cttgcccttc ccgaacggta agaacggtgt tgagtaaaag gactccctgt
    41401 ttagcccatg acactagatt tccatgttga acaggtgcga ttccaagatc atcataaagc
    41461 tctttataga tatttcttaa cgatggcggg atctttacgc caggttgcac agaaaaagat
    41521 aagccatgag cttgattttc tccatgataa ggatcttgtc ctaaaatcac cactttaact
    41581 tcttcataag gagtcaattc caatgcttca tagatatgat acatatctgg atggatcttt
    41641 tgggacgcat attctttttt taaaaattct cttagatgca gataataatc ttttgaaaat
    41701 tctgaggaca aaacttcttg ccaactgtta tgaatgattt ctttcaaaaa ctccacctcc
    41761 taataaaaag aataactcac attttcttca atcacaaggg acaatcttca ccctcagcac
    41821 ttgattaata tggtaaacta ggaacaagaa gcagaaagga acgataatca ttatgatcaa
    41881 attaattgca tcagatatgg acggcacatt attagatgcc catatgagtg tttctcctga
    41941 aaatgccgag gcgatacgtt ttgcaaatga tgccggtgtt gagttcatgg tcgctactgg
    42001 tcgaaactat caagaagctc gcgcagcttt agatgaggta ggaatcgatt gtgcgatgat
    42061 cactttgaac ggggcacagg tatttgataa agaaggaaat tcattgttta ccgtgtcgat
    42121 tccaaataat caagcaatct ctgttttaga tattctagat gctaacggta tttactatga
    42181 agtagcaacc aacgatggtc tttattctga aaatcaagcg aaacgaattg aaagttttgc
    42241 ttccatggtg gctacccatt tacctcacct tacgtataaa atggcgattg ccatggcctc
    42301 agctaagctg gagttattgc acatcacata cgtggacagt attcgtgcta ttttggaaga
    42361 aaagaatctg gaagttttaa aaattatttg ttttcataca gaagggccag ctgtattagg
    42421 tccagttgga aagcagattt ctcttttagg cgatctagcc gttacttcct ctggtcaaaa
    42481 caatatcgaa gtgaaccata aaaacgctca aaaaggaatt gctgttgccc atgtcgctca
    42541 cgagcgtggg atttctttag aagaagtcat gacgattggc gataacttca atgatgtcag
    42601 catgctgcaa gtagcaggag tcagtttcgc aatgggaaat gcagaaatcg aagtaaaaga
    42661 ttatgccaaa tatacaacgg acacaaacct tgaatctggc gtaggaaaag ccatattgcg
    42721 cgcgattgca gaagacttgt agttttatct agaaaagagg gatatagatg tcagaatttt
    42781 tcattcagga aaagcagctt ggacgtgcaa ctcggacaat tgtcaaaaac gaaaaaggac
    42841 attccctgtt tttgatggtt ggtcgctggg gaacaaaggg cgatgcattg tctctttatg
    42901 ccatgaatgg cgatctcgtt gctcacatca agcagataag cctgacattc ggtacgcgtt
    42961 ttgagctgta taaagagttc gaaaaggtgg gaaccatgca gaaaatattc aattggcccg
    43021 gtgactttta ttatattcgt agacttcact ggacagtcca aggtgatatc tataatcatc
    43081 aatatcaaat ccatcatttc aatcagcaaa tcatgaaaat ggataaagca acactttttt
    43141 caggagatta ctatgtattg gatgtctcaa atgatgaaga tgctccagtt tgtatttgta
    43201 ttgctgcggt tttagattac tggctctacc gtaaagacaa aagtcgcgat aagcctgttt
    43261 cttaccgact taacttaagt taaaaagatg tacctgaaat gcggaaatac gcatttcagg
    43321 tacatctttt tagatattct tttgcatatg atcataaaga tgaccactga tcatccgctg
    43381 accgacaact ttgaagtcat ggcgttcgta taatttttta gcctttggat ttttttcatc
    43441 cacacttaga ccgatgacgg agcgattagc tttcttcgcc aatttaggta atgcttctaa
    43501 taatcgcgaa ccgatgcctt gtcctctaaa atcttctgat acactgatag agtccaaata
    43561 ccattcgttg ggaaacgttt ctttatcaat gaacaaacgt gtagacgcat ctagctgata
    43621 tttctctaag ataggtacca gtggctgatc gatgattgat tcttcttcat ctgtataacc
    43681 gtaagcaact cctgcaactt taccgtctat ttcttctacg atacctcgtg aataactgta
    43741 acgataattt ggatcagtga ttgcttcttc taaaatctct aaagtctttt gttcccctgt
    43801 ctgcttcaaa aatggcagtt ccatatcttt tagaatcact aagatcaatg gggcgatttc
    43861 tggagcatct tcttttctcg caaaacgaat catagtttcc tctccttttc tccattctaa
    43921 catgcagaaa aagaaatcgc aaaatcgtta ttttaaaaaa tcttcaatct tttcagtaat
    43981 gaaataacca tgagtaacga ttcttgccgc ttcagttcgg caataagggc agacgactgg
    44041 catttcttta tcattatgtt cgactacatt attgacttct tcgtatgcaa atttttttcc
    44101 gcaattaggg catttcattt tttccatgtt atctcaactt cctttcatct ttactctctt
    44161 agcttactct tatattgtaa aaaagagaag caatatcctt cttctatgca gatatccata
    44221 cctgacggtc ctgttctcca tctattaatc ggactttata ataaggacgt tcatctttga
    44281 ctagaagtgt ccactctttc acttttgctt ttttcccgta gtagctgact gcttccttca
    44341 tcgctgcttg cggttgtttg aactcttccg ttttttctat atcaaaaaga tgttctgcgg
    44401 ccttttccgt gtcatttcgt acggttgttt tcccagtgga tggattgact gctatgactt
    44461 gtccttcaca aaaaaactgg tattcatatg ttgcattcgt aggattctcc aaatcttttt
    44521 caactgcaaa atgaatagca gatatgttat gttcttttgt ttcgtttttt acgattgctg
    44581 ctgctttttt caatgagatc cgatattcct ggtcactaat atcatccatg tgaaaagcgg
    44641 cattgagcag tcccgtcata ccggtaatcc ctattacgat gagggcagca acaccaaaca
    44701 tcattttttt cattcttcat tctcctgttt ctttttttta aagaatatca agaaatgaac
    44761 gaatgaaaaa tcgaatgaaa cgaaaaaaaa gtgacaattt tgttatcttt attcgtgcag
    44821 ttctaatggc aatccgtctg gatcaaagaa aaacgtcatt ttttttcctg taaaggtatc
    44881 ttctctaatc ggttctgatt cgatccctaa ggactgcaat tctgcaattg tttcttcaat
    44941 atcgtctact ttgaaagcaa gatgacgcaa gccacaagct tctggatagt ttggtcgttg
    45001 tggcgcatta gcaattccaa aaatctccaa ttcagcactt cctagtttca gatctaattt
    45061 atagtcatct cgttctttgc ggtaattttc tctgattact tcaaatccta atttattaac
    45121 ataaaaatca cgtgatttct gataatctga aacaatgatg gctatatgat ggatcgttgt
    45181 aaggttcata tgagcactcc tattcttcgt ttaagctcgg atctatatta atctccagtt
    45241 ccattgtaaa tgtgtcagat tgaaaaaaat agctgttttt cctttttata agaaaaaagg
    45301 caaaaaaaac agaaagtagg tcctccaaga cactcttcct gttcccaata taataaatga
    45361 aagtaagaaa ttatattttc aagaaaacaa acagaaaatt atcataacac aaattattta
    45421 taattgaaag actagttttt tttcaagaaa agcatacaat agattgtgtt agtaaataca
    45481 gaacacacta catttagtgt cttaaggagt cctcatacat gaagtcatat tttcagtttt
    45541 tagctcatca gctaaaaggc tggccgcaac aaaattatta tcttttcttt ttcagtcttg
    45601 gctgccaggt catgactctt gtcaatagtc ccatcacttg gctgtccgtt attacattta
    45661 tcggtaccac gcttggtgta ttgtgtattt tatccatcaa tgcggcaaaa tcagtcaacg
    45721 gtattttagg tattttgtct gcgatttgtt ttattatcgt tgggttttca gcaaagaact
    45781 atttaagtat cggtgaacag attgcttata tgatcacatt ggatatcccc gtattgttaa
    45841 gtaaagaatg gaatcataat atggcttcaa aaatccgaaa attcactgga aaaagctggt
    45901 ttatcgcaat catcagtact ttccttgtct atcttgtttc aggattcctg attggtcgtt
    45961 atacagatga tcctcggccg tgggtagatg ccattgcttt ttctattagc ttgactgctg
    46021 gtatcatcag tttcttgcgt tacaacaacc aatattactg gtggttagct tcagggcttg
    46081 ctcaaatggt cctttggtat atttcattta cgcatggatc tgcaagtttg gcgatgttcg
    46141 tgaacagctc tgtttacttg ctgaatgatg tccttgcatt taccgtttca ccttggtata
    46201 accaaaaaga acgtgaacgt atgcaaaaaa tggaagaaga atactacaac gcacaacaac
    46261 acgcataaaa aaactgagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
    46321 cacgtataaa cggcggaatc agaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
    46381 tttgaaaagc aattttcgtg aatttcttct tatttcttgg agttaaacgc ttctgtccca
    46441 gcctctattt tttatagtaa ttccttcaga tactttcttg cctcatcatt gaaacgcagt
    46501 gattcttcta acttctgata atattccaac gcttgtttct gagcttcagt aaaacgataa
    46561 tgttttcctt tccatttgtc gattgtctct tgcacatttc cagattggat atctttcact
    46621 actagttgcc atttttcttt tgagactgta cggtctttct tatcagcaga ttgtatagct
    46681 tctcgttctt tacgcaatac ttttaaaaaa ccatttctga ctatccgagg aatgctgtcg
    46741 aagaaccctt ctgtaaaacc atagtgacaa tattgcttcc aaataaatag atagattttt
    46801 ggttggatga atcgttggtt tttcgcaaaa tattccatct gagcagtgac ttctggtgtt
    46861 cgttgacgtt ttgtctttct caacacggaa ataaatgttt caaaataagc attaggatgt
    46921 actggcactt cttcatatac gatggattgc caatctttac gtaaaaatgc gcgatagttg
    46981 atcaagaatt cttctacatc ctttactaat tcttctgcaa agtcgtcaaa tagttctttt
    47041 tttatctgtc gcttttttga ctgcccattg atgtgttcac ttattttctt gatttcattt
    47101 tccaatgctt gataatccgc tatgtaaatc ggcatttcta cttggcgaaa ttctgcgagc
    47161 cgctgagcta attttatatc cggtaaatat ggctgtttca tccgacgatt ttggtacaag
    47221 acaaaacagt atttttgcca tagtccttca ggaaaatcga tattcttctg cttaagccat
    47281 aataattcgt ctaaggccag atcaactttt gtcattccct gatggatcga agctgccact
    47341 gtcggagaga catgcaggtg atcggcaaag tggttgatgc ctagttttag gatctttccg
    47401 gtttttggcg atttaacgat gtattgcact tttaattctc taccacactc acaatacaat
    47461 ttttgctggc ttcgttttgt tcgaaaaaac ggattgatct tgaagtcaga aaacacccaa
    47521 tctgttgcag ctaaataatg ttcttccata aaacgagagc tgattagata tcggcggtgc
    47581 ttatcgacta gttcccgctg ttttttcgtt aattttgcat agatctccca acgttgactg
    47641 cttgtgtatt gtttagaaat tttgatggga tagcttttgc tgatttcttt tggaaaccat
    47701 tcttcaaaga cggattgcca ttcttccacg gctgtttctt tcctttctaa ttacaaaaag
    47761 ctggctacta acataacgtt agcaaccagc cagtgtttct tgctaaatta gtgagttgtt
    47821 ccggcatttc cggaaatatc ttctttattt tctactgctg cttcaactgc ataagtcaaa
    47881 gaagaagcaa tgtcttctaa agacatagaa ggcgtaccca ctggacgatc gatcacttga
    47941 tcgggtgaga atggcacgtg gatgaaccca cctcgaattg ccgggaattg tgtttcgatc
    48001 aaatgaagca aacgatacat gatatcattg cagacaaatg ttcctgctgt ataagagatg
    48061 tacgctggta gaccatgatc ttgtacattt tttgtcattg ctttgattgg caatgaggta
    48121 aagtaagcgg ctggaccgtt ttcttctaaa gccgtatcga atggctgatt tccttcatta
    48181 tcagggattc ttgcttctgc tagattgatt gcgacacgtt cgaacgagac tgctgaccgc
    48241 ccacctgctt gtccgacaca gatcacgaca tctggttggt gctgttggat ggcttctttg
    48301 actacttgac tgcttttttc gaatacggtc ggtacttcca ctttgatgat ttcagcaccg
    48361 ctgatttcat ctggcatttt ttttactgct tcataggctg gattcacttt gtccccacca
    48421 aatggatcaa aacctgttac tagtactttc atctgttgtt cctccgtccg attgatttaa
    48481 catatggtta tcttttatca taccattttc cttgaacagc gtaaataaag gaaacaatag
    48541 tttaaataac ataacagatc aaacttaata tattcttata aaaaaatagt gttccactac
    48601 aatgttagaa gaggttgcga caattattct gtcacaaccc ctctcttcta ttatttaaat
    48661 agaatcatat aagcaatttg gaaaactagc ataaataacg caataaacaa ctggtttttg
    48721 atcactccgt atttgtcttt catctccagc atcgctacag gcacgatatt gaagttagca
    48781 gccatcggtg tcagcagggt cccacaaaaa ccgcaagtca atgcaaccat tcctattaac
    48841 gctggattcg ctccatgact aaatacgaat ggtgcaccga tcccaaccgt catcacggtg
    48901 atggccgcaa atgcattccc catgattacc gtaaatatca ccatgccaag agcgtaaatg
    48961 atgatgccga tgttcacatt tccttctgga acgatcgtac ctactgcatt ggaaatcact
    49021 gtcccaactc cagcgcttgt aaataccgca cctagagaag ctaaaagcat tggcaacatg
    49081 cttaacggcc ctactgtgcc cagcatatct gctgcatcat ctaaaaatgt acttggtcga
    49141 ttatctcgag aaaagaacat caagattagc atggctgcaa aaacaccgac gcccacgccg
    49201 actaaagcac ctaaattggt aaatagagca aacaagatcg caaagacacc gatactcaaa
    49261 gctggaataa aaattttcat tcctagtttg tctgacattt tttccatata ttcttttgat
    49321 ggaagccggc tttctccttt gctgactttc ttagcaattg ctggtatggt catcgcaaag
    49381 atcaagccac cgttcacgat tgccggaagc catcttcctc ctgcaataac tacccctaag
    49441 accgtccaga aaaaggctga cccataacga tgaggattgg ttttatctgt taaatttctt
    49501 actccagtgt aaatcaatac gagtcccatc acgatataaa ggatctccag taatttatcg
    49561 cctaataaaa tatcaggatt tgtaaaaaag tccatcttat tgcccctttc ctggataata
    49621 tttcttcgct aatttctttt ctttaagata gaagtagaca cttgccacaa ctaaagccgt
    49681 gatagccact ggaacttcca ctaaagcaag ctgtcccaat gttacgtcat atcctagatc
    49741 tttcagtgtt gattggacta gcaacgcccc tgctcctccg atgaatagta cttggccaaa
    49801 gaaccagcag acattttcca tagctgaagc catacctttt agttcttctt catgcttagg
    49861 atggacttct aacccttttg attcgatggt ccccaatgcc atcggtaaaa tgattggacg
    49921 aacaaatcca gcaactccac caaaactcac gttaaaagcc gcaaagatca ttcgaaagat
    49981 cccataaata tctacaatca taccagcaga tactttcttg aatcgcttga tcaaggaagc
    50041 cgctgattct ttcagaccat ttctttcaag cgttcctgtt gccaacataa tgatgataaa
    50101 aatcgccatc ccgcgattgt ttacaaaact tgtacctaaa agttctaaca ttccttgaat
    50161 gccaagaccg ccagtcaaag ctgtaacaac taaagcaacc attacgatca atatcgagtc
    50221 taatttaaat gcaaacccga gaataacaat caagacgcct aaaaggctaa gataattttc
    50281 catatatttt cctccataaa atgacaatat ttttttaatt ataaagcaaa acacataaaa
    50341 aatatagttg ttttctcacg aaatagacac tatttttaaa tatttttcgt tattttttta
    50401 tcacaaaaat tatcttactt attgtaaggt aaagcttttt ttaatttatt gcccgataat
    50461 atatagaaat aaggtctagt tactgttttt tttaaatgat taataaataa caacttcttt
    50521 ctatgaaatt gcaaagcatt catcttcttt tccgctaacg atatatccgt cagtgcttgg
    50581 tacactttac taaaaagtat gagattgccg atatctggat aggtgtgcta tactacagaa
    50641 aaagatatac aatctgccac cgggtaggat aaagaggatg atcattccat taggtctttg
    50701 aagggatgat tttcctcttt ttgtgtattt ttttatagtt aaggaggaat gtaagtatgg
    50761 catggatgga attaatcata gcaggaattt tggaagtatt ctggtcaacg atgatgaaat
    50821 ggagtgatgg tttttctaaa atcaattatt ctttttacac agtgattggc atgattgcta
    50881 gtttttactt tttgtcaaag gcaatcaaaa gtttgccgat gagtctctct tatcctatct
    50941 ggacagggat tggcgcagtt ggttcggtca taattggtgt ggtcttgttt catgataagc
    51001 tgaatctttc cacttggttc tttgtgggga tgctactcat cagtatcatt ggcatcaaga
    51061 ttactagcgg ccactagaca cttggaataa gtactgatca gaaaaaataa gcctttttat
    51121 ttaatattca atttttttat gaaaaaataa gaaataagtc caatgaccta gcgacttctc
    51181 cactcatcag gtgtatgctt gtactatcta tagaaaagga gtgaatattt ttgaaaagtc
    51241 tttcggtcca agaattatcc aagaactttg gtcacaaaca agccctcagc gatgtcagtt
    51301 ttagcttatc ccctggcaaa atcgttggat tagtaggtcc taatggcgct ggaaaaacaa
    51361 cgatcatgaa agctatttta ggactgatcc attactcaga tggaacaatc tgtattggtg
    51421 aaaatgagat ttctccctct agtcataaag ggttggaaca agtaggagca ttgatcgaat
    51481 acccgggtat ctatccattc ttaacaggct atgatcatct gaagttattc tctgaaacca
    51541 acgatgtatc tgctatcgac actatcgtga atcagctcaa gatggaaaag tacattcaca
    51601 aaaaagcgaa atcctactcg ttgggaatga aacaaaaatt gggaattgct cttgccttac
    51661 tgaaccatcc gtctatcgtt attttagatg agccgatgaa cggtttggat ccacaagcaa
    51721 caagagatgt gcgaaaaatc attaccgact tggctactca gggcacctct ttcttgatct
    51781 ccagtcactt actcagcgag cttgaaaaaa tggtcgatga gctgatcctg atcaatcaag
    51841 gaaagatcat caaacaatgt acaatggctg aattgaacca aacagcgcaa gatttcatcg
    51901 ttatctctac aacaaatgat ctagctgctc gaacgatctt gacagaagct ggatatactt
    51961 tggaagatcc tgataaaatc aagcttttaa aagaaaacga agaattatta ggaaccgtga
    52021 tccagctatt gtctgcacaa aatatccatg tgacagacgt ccagcatctg acgaatgatt
    52081 tggaatcttc tctattggaa attctggatt cagtaaaggg ggaagaagaa aaatgacatt
    52141 gcttgtaaaa caagaattat ttaaattgat caagaaaaag tcaactgctg ttttatctgt
    52201 tctattagtt gttttgctca taggaacagc acttctagcc aaaaaataca cgacgatcat
    52261 tgatccagtt gaaatgactg ctcagctttt tagtgccaca tcttggattg tctttatcat
    52321 gattgcagct gcaagcacga tcatttcgat ggaagcacaa tatggtacat tgaaaaatct
    52381 cctttatcga aagtattctc gaggagaaat attagtaagc aaatggatca cattagttat
    52441 ttattctgtt tatttatacc ttttagctat tattgtcact gtgttgatga aactgattct
    52501 tttcccttcg atttccttca cggagaaggt ttctactggt caaacactga tccaaagctt
    52561 gttcacttat actttgggta gttatatcgg tttatggctg attttaagtt tagtgttgat
    52621 gattgcttgc tttatcaata gttccggcgc atccatctca gcaggaatcg tcttttattt
    52681 tgcttcgtca atcatatctg gtatccttat tgcactcatc caaaaatggg agtggatcaa
    52741 atggaatccg atcagtatgt tgaatctgca aaaccaaatt ggaaacgaag agatcatgaa
    52801 gcaattgact catctttcta ctaatcaaat gctattcggt aatttagctt acatcgtttt
    52861 gttcttagca ttaggatatc tcgtatttaa gcgaaaaaat gtatgatgac tcatcaaaaa
    52921 agaggctgtg aaagagacgt tcagccttat tgccgattgg ctgtctctta aacaccgttt
    52981 attctgaaaa aggagctgtg acaacatttg tgtcacagct cctttcctag tatgtttttg
    53041 aataaacgac aggctcacaa cctctcttta tacttctttt atacttctat acgtgaagca
    53101 tcccagtggc aaataaaacc attgcaatca ctccgaccat cgtcacacct ataagtaaaa
    53161 tcttatctaa gtggatatgt tgtacaggct cgttttgctc cttacgtgct ttgaggtaaa
    53221 aataaatgcc tggcaagaaa agaacaagaa caagcattac ttcattaaag gctacgacta
    53281 gcatagccca tacttgaaag gcacttgcta aaagccctac taaaaattgg acccattctt
    53341 tccgctgata agaaagtttc atttggtaca atcctaagaa aagccacgtg aagaaaatag
    53401 ctgctgtacc aagtgtgtaa gcaaagttat aagcactgtc agtgaataat aatgaaagta
    53461 aaaagacatt cgtacataac gtcgtcaata cgatagcata agttggtgcc ccatttttgt
    53521 tgagttttcc ccatgcttta ggcaaaagtc cgtcttcagc catgtttccc attgtttcaa
    53581 tcggtaagat cgtccatgag agccaaacac caactaaaga aatgatcaaa ccgccgttga
    53641 tcacgactgc tccccattta ccaaccatca tttcaaacac atagcccata gcaggctgcg
    53701 ccttgatcgt ttgcagctga ttcatcgtca ttgtcccata tggaagcatg gaaaccaaga
    53761 cgtaaataat aatcaagccg aatatcccta gaatcgttgc tttcccaaca tctgattgtt
    53821 tttttgctcg tgaacctacg acagaggctc cttcaatacc aacaaatacc caaagcatca
    53881 ccatgatgca actcaatact tgactggaaa acgggatcgg ctgtccattc gcattcaata
    53941 taatgtcgct agaaaagtta tcaatgaatt gttggatcga aaacgtgctg atagctacta
    54001 caataaataa aaataatggc actaatttac agaatgtgac gatagcattg acgaaactag
    54061 cattttcgac tccccgattc actagccatg catatcccca taaaaagact gtactcatca
    54121 cgatcgatgc cagattttgc ccaccttcaa atactgggaa gaaatatcct aatgcgctca
    54181 tcaacaatgt tgcaaaagct acgttgccta accagcatgt gagccagtaa ccccaaccgc
    54241 tgataaaacc agcgaaacgg ccaaaacctt cttctgcgta actaaagatc ccgcctttta
    54301 aatctggacg ttttttattg atattattga tcgacaagga gagcatcaac attgctgttc
    54361 ctacgagtac ccaactgaaa atagctgggc cgccagccga tccggagatg tccgtcataa
    54421 taccaaatac gccacctcca atcgctgaac caattactaa ggcagcaagt ggaattaaac
    54481 caattccttt tttctgttgc tctgtatcca tgtaagcatc ctccaaaata aaaccgcttt
    54541 tatcaaaaag tataacaaac cttttttatt tttacttaat taataaaaac tttaatagaa
    54601 atataattct accggctttt ttctcattat acaagcaaag cgcctagtcc ctttccaaat
    54661 tacaccacca aaaaacttaa aaaagatgct atactaaaag gaaagaattg gaggatgatc
    54721 catgaacatt ttatatatat cgatttcagg aaatacgcgt gcctttgcca aacatctagc
    54781 agaatatgct gaaaaaatgc atgcagaaga tcctgtcaat cctgaagtta ctttaaaaga
    54841 aatccatgaa aatagtgatt ttacgaaaga aacagaacca ttttttactt tcgttcctac
    54901 ttatttagac ggcggaaatg gtttagataa cggtgataca gaaatcttga ccgaaacgat
    54961 gcgtgaatat ctggaacatg aagacaatca taaactttgt cttggtgtag tcggtagcgg
    55021 aaataaaaac ttcaataatc agtattgtct aacagccaaa caatatgcac aaacatttgg
    55081 ctttcctttc ttggcagatt atgaattacg gggaacacca tctgatgtga cacgaattta
    55141 tcagatttta gcagaaaaca ataaataata aaaaagaaga agctgaaaaa tagatttcag
    55201 tttcttcttt cgtttctatt gtttctatca tattaagctt tgacatccat cacaggtgct
    55261 tttgcttttg ctttacctgt tttttccaag acaaagtttt ttacacgatc catattggtt
    55321 accaagacga tcatggatgt ttctttgcct gctgctttga tcgcttctaa gtcagcttct
    55381 gctacaagcg tatcaggtgt cacttgctgc ccttctgtta ctttcacatc aaatggcgta
    55441 ccgcctaagt caactgtgtt gattcccata tgaagcaaga tttctaaacc atttggcgtt
    55501 gtgatcccaa tagcgtgttt cgttgggaag acagagctga ctaccccagt aactggagaa
    55561 tagatatgtc cgttttctgg ttctacaaag tagccttcac ccatcatctt ttgtgcaaaa
    55621 actggatcac ttgctttatc taaagaaaca actgttccgt cagcaactga tgagaggata
    55681 gtcacacttt cagctgaagt ttgaggtact actttttcag gcacttcatt tactgtggct
    55741 gtttcagtcg ctaattcttc tttttctaaa cggtctttag gaataccgaa gaagaatgta
    55801 gcaacgaagc caccagcata agctgccaat aatccaagga tgtagcctaa ccatttaccg
    55861 tcagagatca atgggatcaa agcggctcct gaaggtccaa tagcgatagc cccgacattt
    55921 ccgatcattc cgatcactgc cccgccgata cctccaccga tacaagcagt aataaatggg
    55981 cgtcctagag gtaatgttac tccgtaaatc aatggttcgc cgattcctag gatcccgact
    56041 ggtaaagctc ctttgatcat ttctgttaat tctttatctt ttttgcagcg aatccataat
    56101 gccaatgctg cacccacttg tcctgcacca gccatcgcaa gaattggaag caatagcgta
    56161 cgaccagttt catcaatcat ttgcacatgg attggagtca aaatttgatg cagtccgaac
    56221 ataaccatcg gcaagaataa tgtgcctaga acgaagccgg caaatgctcc accaacgcca
    56281 agtacccagt tgatcccacc gaccatgcca tcagaaatga aacctgctaa aggcatgatg
    56341 aagaaaattt caatcagacc gatggcaatc aacgaaagag ttggtgtaac gatgatatct
    56401 accgcatcag gtactacttt atgcagtttt ttctcaacga ttgacaatag ccaaacagcg
    56461 aagatgacac ctaggatccc accttgtcca gctgcaagag cttcaccagt aaataagttt
    56521 ttcagtggtg cttccggatt catccctgtc agcatcacaa cggccccgat aactccacca
    56581 agagttggtg ttgcaccaaa aacttgtgca gcattgatac ctgtatagat aactaggtag
    56641 ctaaacatcc cgtttttcaa gatgttcatc acatcgatat attgttgcca tgttgctgca
    56701 tcaagtgttc cggctgtgac taagtttgct aaaactgcgg caatccctgc cacgatccca
    56761 gtaccgacaa atgcaggaat cattggcaca aagatattag aaatgtcttt taataccgat
    56821 ttgattttag atggtttttg tttcgatttt tgtgccgctt tcatttcttg tgcccgttcg
    56881 tttacacggt cttttccaga tgctgatgaa tttgctgtag ctgtacttac agaagaaagt
    56941 ttttctccta atttcacgcc tgccaaatca accatttgct gtgctacttt gttcactttc
    57001 ccaggtccga tgatgacttg aagctgttca tcttctacta caccaagcac tcctggaatc
    57061 gcttttaatt cagagacatt tacaagatca tcattgatga tcttcatacg cacacgagtc
    57121 atgcagtgga caatgctttc tacattgccc atgccgcctg cccctttata aatttgtttt
    57181 gccagttctt ccaaagtcag ttcgtttttt gccatggtct tttccccctc ataaggtccc
    57241 ttttacaaat ccgtttgctt cagctaattt tgcttgagct tcctctttat cactatttgt
    57301 caaaatcatg acgatagcca gcttaacatt ttgatctgct tcttcaaagt acttttcagc
    57361 tgtttcgtaa tcagcttctg tcgcttgcat gatgattcgt tttgagcgtt cgattagttt
    57421 ttcatttgtt ggtttgacat cgaccatcaa gttattgaat actttaccta taccaatcat
    57481 actgatagta gaaatcatat tcaagataag tttttgtgct gttcctgact tcaatcgagt
    57541 agagcctgtc aaaaattctg gacctgcatc gacttcgatt gggaaatccg cgtgtttact
    57601 gatttcggct tgtttattgc aagaaatcgt tcctgtgaca gcaccgatcg attttgcgta
    57661 atctaatcca ccgatcacat atggtgtacg accgctagct gcgataccaa taactatatc
    57721 attttttgtc aaattcagct tgattagatc ttcttgccct agctcataag aatcttcggc
    57781 cccttcgaca gctacagtca ttgcttcttc tccaccagca attagaccta tgaccatttc
    57841 cggttctaca ccgaatgtcg gtacgcattc agcagcatct aacacaccta gtcttccact
    57901 tgttccggct cccatataaa tcagacgtcc acctttttta aaggactcaa tcgtgttagc
    57961 aatcactggt tcgatacttg tcagttcttt ttcaaccgca gcggccactt tctgatcttc
    58021 tttgttcatt agttgaacgg cttgagaaat actcatctca tctaatccaa aagtttcttg
    58081 gtttctgcgt tccgttgtta ggttctctaa attgattttg ctcattttta tcttccttct
    58141 tttcttataa attgatagtt cataccagct ttgatttggt tgatcaatgg tagctcttct
    58201 ttgataactt tagctactac attcactttt tcatccgctg gcaaatcata tttggttaat
    58261 tggatctcac ccatatagcg caaatacttt tcgttatcta aagtaataga ccctttcata
    58321 cgcgtagctg tatttcttgc tgggataggc ggaatctctc gaaaacgtgc atctacgctt
    58381 cgtatgacat ctcttgcatc gtcttgccga ttcgtatgct tccctaatat atattcgtaa
    58441 aattcttcat ctacaggttc cgtatggagc aagactgtct gttctttttg gaaagaagca
    58501 aattgctcct gaacgttttc tgacagacct gcatctccga tgtaaacaat atccgtgtta
    58561 cttgctaata gatctagagc gcctgcaaga ggatggatcc ctcgatgttc ttccaaagtt
    58621 ggcaatcctt gatatagtgg gcctctcaac tctgtatctc ctggtacaaa accttggaca
    58681 aggagacctt gaattttgag ccattgattt tttttctgat accattcttt gtccagccct
    58741 gtttcgggac gtggataata attatgccaa gcttcaatct gtgagaaatc agcttctgct
    58801 tctttcaatt catcgatatc tttaggcgtg atcgtgctag cattcaaact gattttcaat
    58861 tgatgtgacc agtcggcaat ctgctgattg gaaatatgat aatccattct taaacctgtt
    58921 acaccaattg ctttcaaagg cgttagatct tctaatgaaa aacccgctcg ttccaatgcc
    58981 tctcctgaaa tatcgaccat caattctagt ttattctttt tcgcttcgct tcctagttcg
    59041 atcaatcgct tttgatatgc cgaaacatca tcttctggta tatgcatgga agtgaaaata
    59101 ccgacaaagc cattggatgc catttggcgg atatactgtt tcttctcttc tgacaaagaa
    59161 tcgttcataa atattgaaaa gccaaacata ttatccctct ttttctgtat ccgtcttcat
    59221 ttctatgact agagtatata tctaaagaaa caaaatttca atacttttat taaaataatt
    59281 aaatttcatt tctaaaggtt gagtttatat tatcaaaaga attgtcataa cctaaaaaag
    59341 gcactgctaa gcaaaagcag tgccttgaaa tattttattt aaataacaca aaaacataat
    59401 tactcataag aaaccaattc tgtattatct accatgaatg taaagaaccc ctcattaggc
    59461 atcggatgaa aataagcatt gatcactctt atcaaggcat tatggcaaac taacagatag
    59521 acattttctt cgtcttcgat gcattctttc aataaaggaa cgatccgtgc atacacatct
    59581 aagactgact ccccatttgg gaatcgtaca gcgaacgcta aacgtgcttt ttgaaagttc
    59641 tcatctttgc ttggcagtcc atcataatcc ccaaaatcca tttctaccaa tcgttcatca
    59701 acggtcagcg gcaagctgag acgatcagca actgcttgtg ctgtatctct tgctcgttga
    59761 agaggggaat ggatgatttt cgtgattgga acaggcaaat ctactacctt ttctgctagt
    59821 ttctgtgcct gcatctgtcc tttttcagtc agcggcacat ctgcccgtcc gcaaactaat
    59881 ccttgtgcgt tccaagttgt ttctccgtgt ctcgttacat aaagcatgtg attctctcct
    59941 tcgttggcaa agttcccctt aaccatacct tgttgttctt gagattacaa gggttagatt
    60001 ctctcaagag aaataatctc agtagatact ttatccagga aggtttgatc atgttccact
    60061 agtaacatgg caggtttcac gttcaagatc agttgttcta attgttcttg attgaagaca
    60121 tccaaataat tcaatggttc atcccatgta tatagttcag ctggctgtga taaagattta
    60181 gccaattcca cttttttccg ttggcccata ctcatctgct cgatcttgtt atgaaaaaca
    60241 tctctttcca tcccaagctt tcggaggttg ttcaaaaatg cttggtagtc tacttggttt
    60301 ttctctgcaa attccgccaa cgttcctcga ttgtcttcat aattttggct tgcataacta
    60361 atgctcagat gttttggctg gtatttttct cctataacct tgccgttgaa tgcccccaga
    60421 agataatgga taatggatga ctttcctgca ccgttaggac ctgaaatcgc cacccgctga
    60481 tgaggctcga ttgtaaaatg aattggctcg aataacagat tttcataccc taattgaaga
    60541 tcttctacgc ttaaaagtcg cttatggtga gacgcttggc tattcatcgt caaagaatcg
    60601 atatattcga tatcttttaa tagtttttct ttctcgctga tctgggtctc catccgattc
    60661 acgattgctt tggatcgttt catcgtccgt gcagcatcag cacccactgc tcctttattc
    60721 actcgtctag attcagtatc gatgaatccg acttgtttct ttgttttatc tccttctcgg
    60781 gaacgagacc attcggcttt ttcagctgct gttttcttta gcctactgac ttcttttttc
    60841 aatttttcat tttgagccat ttcaaattca tcacgaagtt ttttctgttc ttcatagata
    60901 gagaaattcc cttgataaag ttccagttga cttttttcga ttgctaaaac atggtccact
    60961 acttcatcga taaatccccg gtcatggctg accacgatga agccttgttt tttctttttc
    61021 aaataagccg ctacttgttt tctaccagag atatccaaat gattcgttgg ttcatcgatt
    61081 aacgggaaat gagtgtcatc cacaaataaa agtgccagta ggacttttgt cttctctccc
    61141 cccgatagtg tgctgaattc tctccataag atttcaggat ctgtttgcat caattggagc
    61201 tctctttcga tttcccatat ctcaaaatcc gtaatatcat ttaacacgta ataggttaaa
    61261 cgttctttat cttttgtctg ttgcgggaaa taggcaaatt cttgctgatg gattacttgc
    61321 ccttgataag gtagtttgtt ttgtagaata ttcagtaaag ttgtctttcc tcgaccgttt
    61381 cgtccgataa gtcctagttt ccattgtgtg tcaaaattta gatttgcttg ttcaaataat
    61441 aatgtgcctt ggctgtcgta gccgaatgtc agatttttta tttcgatttt agacattttc
    61501 catcacctcg taaaaaaaga ccagtcctat tttcagaact ggctaaagag gatgcaaggg
    61561 ttggatacac acaaaaagac ccatacatcg aaaaaaccaa gccaatcccg aaaatctgca
    61621 cactgcttcc ttgtctccac aaggtacagt ctgaatgcag ctttcatcaa tcgacttaat
    61681 ttttcaatgc acggatccct cactttgtcc tatatttgaa attacgatag cataaagaaa
    61741 atgattttgt caactacttc gggaggatgg tgaaaaatct gctaagtgtc tgtcctaaga
    61801 tttttgtttt ttctaatgtt tgcctgtgtt ccattgcttc ttctaaacct attggaccca
    61861 aatgaatact taagacaatt gggagaaaat cagaaagtcc ttctagctct tggtctctac
    61921 ttccgcaaat agcgattatt ggttttcgat atttttttgc aagcactgct acaccgtatg
    61981 gaacttttcc gtggatcgtt tgctgatcga tccgcccttc tcctgtaatg atgacatctg
    62041 cttgttcgat ctttttttcc aatccagtga tttggctgac taaatcgaaa ccagaaacca
    62101 tcttcgctcc caacaaaaaa agggctccac caataccgcc tgctgctcca cttcctggaa
    62161 tatcatctaa atccacctcc cagaaatctt ttatttgctg agcaatcttt cctgcttgtt
    62221 tatctaagaa agctagcgtc gtatctattc ctcctttttg tcttccaaat tgccaagcag
    62281 ctccttgttg acctgtatat ggattggtaa catctgcagc gataatcaat tttactttag
    62341 aaacaagaac gtttcttcga tcaaaatgaa gttttttagt agataataaa ggatttcctt
    62401 ctgtgtaccc ttctaaagaa gcacccaagg attggagtaa tcctagacca ccatcgctac
    62461 acccgcttcc tcctaaagtg acaataattt gttctacttc ttgaagcagt gcatctttaa
    62521 taagttctcc taaggcataa ctggaagcaa gtcggatagt ttgatccgtt ggtttatcaa
    62581 gtagatctaa ccccaatgct tgtgcagatt cgatgactgc ggtgtttttt ccttgccaag
    62641 ttgtcaataa atagctgatt tttcgctgac ggaataagag atccatccca tcaatggtac
    62701 gccatgttcc cctgagcgcg taatagattg cttctgcagt attttctccg ccatctgcaa
    62761 tcggcacatt gatttttctt cctttacgat cttcccagcc atttagaact gcctgattta
    62821 gttcaaatga agaagcactt cctttaaatg aatcaattgc cgccaaaata tccatctgta
    62881 tccctcactt tcttcttaag taagacactt tttttcattt ttttcaaaca aaacaatgct
    62941 attttcttcc atttttctat atactaaaag ggaactcatt gaataaagga gagaaaaaac
    63001 atgaaaatcg caatagctgg ggccggagca atgggcagcc gaatcggatt gatgctacat
    63061 caaagcggaa atgaggtcct cttgatcgat cgttggcctg ctcacattga agccattcgt
    63121 acaaatgggt tgattgctga ctttaacggt aaagaagtgg tcgcaaaact gccaatctat
    63181 tcaccagaag aaatcatcga aagcaacgaa cacgtggact tgatcgtagc attaacaaaa
    63241 gccaatcaat tagatgatat gttttgttcg atccaatcca ttattactga caatacttat
    63301 gttttgtgtt tactaaatgg attaggtcat gaagatgtac tggaaaaata tgtgccaaag
    63361 aaaaatatct tatttgggat cacgatgtgg acagctggtc tagctggtcc cggaaaagtc
    63421 acattgttag gtgatggaga aatcgaacta gagaacttgg aaccagaagg cgaagcattt
    63481 acaaaaaaag ttgtagaagt ctttcaagaa gcaaatttaa atccgatcta tagccataac
    63541 gttcgttatt ccatttggcg aaaagcttgt gtaaatggaa cattgaatgg tttatgcacg
    63601 attttggatt gtaatattgc tgaactaggc gcgcaaaaag cggcagagag catggttcga
    63661 acaatcgtat ctgaatttgc ttctatcgca gcaaaagaag gaatcatact agatcaagaa
    63721 gaagtgtacc aacatattgc ttctacttat gatcctgaca acatcgggtt gcattaccca
    63781 tctatgtacc aagacctgat caaaaaccat cgtttgactg aaatcgacta catcaatggt
    63841 gccatttggc gaaaaggaca aaaatatgat attgctacac catattgtgc ctttttgacc
    63901 caattagtcc atgcaaaaga aggaatcctt ggcgcagaat aaaaggctct ctgatgagaa
    63961 tcagctgaat cagcagctgg ttctcttttt ttaagcccaa taaaagatca aaaagaaaac
    64021 acaactctca tgatgccata agaattgtgc ttgtttgttt cagtacataa tctcttcaaa
    64081 atgtaggttt tttatttgtt ttcaacagag gagaggtcat attcgtattg ctcataaata
    64141 gtggaatcgt tagtgataaa tcgttttgcc caattttctg catcataaaa ttccaatagc
    64201 tctgatagga ttggcttaaa tggtgtatgc agaaattccg gtaattcatc taaatcacgt
    64261 tctcgaagca aaaaaatatt ttcaggacga taaaaatgat aatgagcaat cgcttgatta
    64321 tccgtcacta gtttcttttg aaaatacatg gattcaaatg tccgcaaagt cactccttgc
    64381 tgaccctccc gcaacagttc cagaatcccg cgcgattccc ctgttttttt caggtactct
    64441 tcataagtaa gatatccagg ttgttcattc cctcgatgtg gaagaatgaa atagttggtc
    64501 gtaacatctc tttttataaa ttcctgctta tagctttctg ctcgttcttt tctcccatta
    64561 ttcgttgcac caaaaaatag atccgtcttt ggttcgctag aagaaaaatt catatgtttc
    64621 gaataaaatg aactattatg tttcagccca agttcttttg cttccaaaaa atcaaaatga
    64681 taaatctcat cgattgccgg ttcatcttgt gcctgcttca gcatcgcctc gtgttctgct
    64741 ttgaagtgat tccaaaaaaa taacacaatt tttgcattgg taaatgggcg cacgcgtcga
    64801 gccatctcca tatcaaatcc ataatctaaa aaaacgatca gctgataatt tgccaactgt
    64861 tgtctagctg atttaatgaa ttccgactgt agtaaatctt ttccggacca aacatctaaa
    64921 gagcggatgg aaggcaaaaa ataaagtgat ttattttttg caactagcag tacgtcattt
    64981 ttgttcatct tttccgcctc actttctctg tcactttttc ctatgatagc ctgtttaaat
    65041 agtaaaaagc cagtaaaacg tttcctatat ttgtatgaaa atttaacatt tgtatgaaaa
    65101 tatgatgaaa taaatcattc ttctattaaa attcgcctaa aagttttctg aatagttgta
    65161 attgtctgaa aatttgttat cattagtcat tgttaaatat tgctatttat tcttaatata
    65221 aattttgatt gtgaatttaa aagaaagagg tcatttatgg aagataaaaa attgaaaaaa
    65281 caacttagct ccagacatat caccatgtta gctttaggtg gagcaatcgg tgcaggtcta
    65341 tttaaaggaa gtggcgaagc gattgggatc gccggccctt ctgtattgat tgcttttttg
    65401 attggtgggc tgattttata tatcgtgatg aaaggtctgg gaaaaatcgt tttaagcggt
    65461 ggcgacacgc atcacggatt atctggtttg atacgccctt atctaggtgc tcattctgct
    65521 gattttactg attgggtcta ctggtctatg tggattatca atatcatcgc tgaagctgtt
    65581 gcagctgctt cctttttgca gttgtggttc ccgcacattc cagcctgggt atttgttttt
    65641 atgttggctg ttttaactac gatcatcaat ctttattcgg tacggttatt cgcagaaaca
    65701 gaatattggc tggcttttgc taaaatcagt gtcattatct tactgatcat ttttgctact
    65761 tatcttgtcg gtcagcaaat gttgggcaca ggcgttttcc ctacccttca gcaattgact
    65821 gaccatggtg ggtttactcc tcatggtatg aagggaatca tcagttcttt gcttgtagtt
    65881 atctattctt acggcggttc tgaactgatt gctatcacgg taagcgaagc agatgatcct
    65941 aaacgtgcga ttcctaaagc aatcaaaggt gtcatgggaa gaatcatttc cttttacatc
    66001 attcctttgt ttttgctttt gattcttttt ccttggaata ctttagcagg cacgaatgtc
    66061 agtccttttg tgatggtatt tgaaaaaatg aatatcccat ttgcagcgga tatcgtcaat
    66121 tttgtgattg tccttgcttt gttctcatct atcaattcgg gtgtgtatgc atcttcaaga
    66181 attttgttct tccgtttgaa ggatcgaaaa ggttcctcta gaaaactagc tgttttaaac
    66241 aagcatcaag taccgcaacg tgctgtttta ttttgtacca gtgttcttta tcttggtgta
    66301 gctctttctt attttgtcgg tgacaaatta tttggctatc tggcaggttc cctttcgtat
    66361 acagtattat tgatctggat cattatcagt gcggcatcct ttgtactggc actaagaaaa
    66421 ggaacattat gggataaagg aatcagcttt tttgcattag ctgtcttagg attgattttc
    66481 cttggtatcc tgttcacgaa ctccattggg gtaactgtat tgactggcct gctttacctg
    66541 ttcatttatt tcagttatca aaaaaagaat gatgcgttcg tattgacgaa tgaatgaaat
    66601 cgatagaaaa ttttatttta tttatgtgga atagatggag ctattgaaga aaggagttgt
    66661 gacaaaagtc ttgttgcaac tcctttttcc gaataaacag tctcatgacc tcttggtaaa
    66721 cggtgttcaa aaaccctctg tgcaaaataa ttgattgttc tccatgctca tgacatgata
    66781 gaagaaagga atgaaaaaaa ggagtttgtt ttaaatgaaa aagtattgga agtttttgtt
    66841 gatgatcggt gtttcaacgg tagttatgtt tggtatgatg tacttgaatg tctatgcgct
    66901 cgatcatatc ttcttcagtc gtacacgagt attcatggcg ttgatgatgg gtgcagtcat
    66961 ggcagtcatc atgctgttgt tcatgtggaa aatgtatgaa aacaaaaaga taaatgttat
    67021 gatcttagcc gctagtcttc ttgtttttgc cggttccttg tttatggtac gaagccaaac
    67081 tactgtcaac gacacagctt ggatgaaggc catgattcct catcattcta tcgctatcct
    67141 ggcaagtaaa cgagctgatt taaaagatcc tgaagtaaaa aaattagcgg aagaaatcat
    67201 tcaggcacaa gaagaagaga tcgacaaaat gaaaaaaatg atcaaagaaa tggaatgacc
    67261 attatccgtc caaataaaaa aagaggatgt tccagaagtt atcagcttca agaaataaga
    67321 agaagctact tctggaacgc cgtgaataag gggttgtgaa aaagctgccc tgcatcaagt
    67381 aataagaaca gccaaacaaa aatagcttct cctatttttc gtttggttag gcttattacc
    67441 gcagatgcaa gcttttgaac accgtttatt aagaggttgt aaaatcagcg ttctgacctg
    67501 agtattagag agacaaatgg aaaaatagtt cctcatattt tgccattttt tgagctaata
    67561 ccgagggtca gctgattgaa caccgtttat cgagaggttg tgaaaaagtc gcttagtttc
    67621 gcgtattaag aaaaattttg tgaaatggct ttccacattt ttgtataaat ttggcttaat
    67681 accgagaaac tggcttttga ataccgttta ttcgtaaaag ggattgtgac aaaagtcttg
    67741 tcacaatccc tttttatgtt
//
LOCUS       DAFEOM010000009        62466 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.009, whole genome shotgun sequence.
ACCESSION   DAFEOM010000009
VERSION     DAFEOM010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.009"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            372..3179
                     /locus_tag="LP524_000686"
     CDS             372..3179
                     /locus_tag="LP524_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL2233760.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            3370..3861
                     /locus_tag="LP524_000687"
     CDS             3370..3861
                     /locus_tag="LP524_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2233761.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            3964..4953
                     /gene="manX"
                     /locus_tag="LP524_000688"
     CDS             3964..4953
                     /gene="manX"
                     /locus_tag="LP524_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBL2233762.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            4986..5789
                     /locus_tag="LP524_000689"
     CDS             4986..5789
                     /locus_tag="LP524_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBL2233763.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            5810..6724
                     /locus_tag="LP524_000690"
     CDS             5810..6724
                     /locus_tag="LP524_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBL2233764.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            6807..7181
                     /locus_tag="LP524_000691"
     CDS             6807..7181
                     /locus_tag="LP524_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HBL2233765.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            7320..7565
                     /locus_tag="LP524_000692"
     CDS             7320..7565
                     /locus_tag="LP524_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBL2233766.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            7737..8801
                     /locus_tag="LP524_000693"
     CDS             7737..8801
                     /locus_tag="LP524_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBL2233767.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            9047..9472
                     /locus_tag="LP524_000694"
     CDS             9047..9472
                     /locus_tag="LP524_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBL2233768.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            9465..9914
                     /gene="nusB"
                     /locus_tag="LP524_000695"
     CDS             9465..9914
                     /gene="nusB"
                     /locus_tag="LP524_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBL2233769.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            10011..10865
                     /locus_tag="LP524_000696"
     CDS             10011..10865
                     /locus_tag="LP524_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HBL2233770.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            10866..12203
                     /locus_tag="LP524_000697"
     CDS             10866..12203
                     /locus_tag="LP524_000697"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBL2233771.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            12208..12432
                     /locus_tag="LP524_000698"
     CDS             12208..12432
                     /locus_tag="LP524_000698"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBL2233772.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            12434..13324
                     /locus_tag="LP524_000699"
     CDS             12434..13324
                     /locus_tag="LP524_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBL2233773.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            13333..14151
                     /locus_tag="LP524_000700"
     CDS             13333..14151
                     /locus_tag="LP524_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HBL2233774.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            14258..14707
                     /locus_tag="LP524_000701"
     CDS             14258..14707
                     /locus_tag="LP524_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBL2233775.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            14725..16407
                     /gene="recN"
                     /locus_tag="LP524_000702"
     CDS             14725..16407
                     /gene="recN"
                     /locus_tag="LP524_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBL2233776.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(16462..16578)
                     /locus_tag="LP524_000703"
     CDS             complement(16462..16578)
                     /locus_tag="LP524_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HBL2233777.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(16651..17607)
                     /locus_tag="LP524_000704"
     CDS             complement(16651..17607)
                     /locus_tag="LP524_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBL2233778.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            17778..18146
                     /locus_tag="LP524_000705"
     CDS             17778..18146
                     /locus_tag="LP524_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HBL2233779.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            18317..18748
                     /gene="mraZ"
                     /locus_tag="LP524_000706"
     CDS             18317..18748
                     /gene="mraZ"
                     /locus_tag="LP524_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBL2233780.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            18769..19728
                     /gene="rsmH"
                     /locus_tag="LP524_000707"
     CDS             18769..19728
                     /gene="rsmH"
                     /locus_tag="LP524_000707"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBL2233781.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            19733..20134
                     /gene="ftsL"
                     /locus_tag="LP524_000708"
     CDS             19733..20134
                     /gene="ftsL"
                     /locus_tag="LP524_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBL2233782.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            20135..22327
                     /locus_tag="LP524_000709"
     CDS             20135..22327
                     /locus_tag="LP524_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBL2233783.1"
                     /translation="MSLKNKIRRFMEKKNLNPINNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            22353..23315
                     /locus_tag="LP524_000710"
     CDS             22353..23315
                     /locus_tag="LP524_000710"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBL2233784.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            23329..24690
                     /locus_tag="LP524_000711"
     CDS             23329..24690
                     /locus_tag="LP524_000711"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBL2233785.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            24716..25804
                     /gene="murG"
                     /locus_tag="LP524_000712"
     CDS             24716..25804
                     /gene="murG"
                     /locus_tag="LP524_000712"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBL2233786.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            25819..27039
                     /locus_tag="LP524_000713"
     CDS             25819..27039
                     /locus_tag="LP524_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HBL2233787.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            27195..28520
                     /gene="ftsA"
                     /locus_tag="LP524_000714"
     CDS             27195..28520
                     /gene="ftsA"
                     /locus_tag="LP524_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBL2233788.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            28543..29784
                     /gene="ftsZ"
                     /locus_tag="LP524_000715"
     CDS             28543..29784
                     /gene="ftsZ"
                     /locus_tag="LP524_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBL2233789.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            29801..30478
                     /locus_tag="LP524_000716"
     CDS             29801..30478
                     /locus_tag="LP524_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBL2233790.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            30492..31091
                     /locus_tag="LP524_000717"
     CDS             30492..31091
                     /locus_tag="LP524_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBL2233791.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            31106..31393
                     /locus_tag="LP524_000718"
     CDS             31106..31393
                     /locus_tag="LP524_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBL2233792.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            31442..32224
                     /locus_tag="LP524_000719"
     CDS             31442..32224
                     /locus_tag="LP524_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBL2233793.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            32245..32952
                     /locus_tag="LP524_000720"
     CDS             32245..32952
                     /locus_tag="LP524_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBL2233794.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            33214..36012
                     /gene="ileS"
                     /locus_tag="LP524_000721"
     CDS             33214..36012
                     /gene="ileS"
                     /locus_tag="LP524_000721"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBL2233795.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(36061..37587)
                     /locus_tag="LP524_000722"
     CDS             complement(36061..37587)
                     /locus_tag="LP524_000722"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBL2233796.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(37602..38249)
                     /locus_tag="LP524_000723"
     CDS             complement(37602..38249)
                     /locus_tag="LP524_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HBL2233797.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(38433..38762)
                     /locus_tag="LP524_000724"
     CDS             complement(38433..38762)
                     /locus_tag="LP524_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HBL2233798.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            38939..39667
                     /gene="sfsA"
                     /locus_tag="LP524_000725"
     CDS             38939..39667
                     /gene="sfsA"
                     /locus_tag="LP524_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBL2233799.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            39683..40696
                     /locus_tag="LP524_000726"
     CDS             39683..40696
                     /locus_tag="LP524_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL2233800.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            40696..41973
                     /locus_tag="LP524_000727"
     CDS             40696..41973
                     /locus_tag="LP524_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBL2233801.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            42036..44738
                     /locus_tag="LP524_000728"
     CDS             42036..44738
                     /locus_tag="LP524_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL2233802.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            44890..45207
                     /locus_tag="LP524_000729"
     CDS             44890..45207
                     /locus_tag="LP524_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2233803.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            45237..45557
                     /locus_tag="LP524_000730"
     CDS             45237..45557
                     /locus_tag="LP524_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBL2233804.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            45665..47125
                     /locus_tag="LP524_000731"
     CDS             45665..47125
                     /locus_tag="LP524_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL2233805.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            47193..47414
                     /locus_tag="LP524_000732"
     CDS             47193..47414
                     /locus_tag="LP524_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233806.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            47445..47627
                     /locus_tag="LP524_000733"
     CDS             47445..47627
                     /locus_tag="LP524_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HBL2233807.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            47627..48040
                     /locus_tag="LP524_000734"
     CDS             47627..48040
                     /locus_tag="LP524_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBL2233808.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            48163..49344
                     /locus_tag="LP524_000735"
     CDS             48163..49344
                     /locus_tag="LP524_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2233809.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(49415..49579)
                     /locus_tag="LP524_000736"
     CDS             complement(49415..49579)
                     /locus_tag="LP524_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233810.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            49733..49806
                     /locus_tag="LP524_000737"
     tRNA            49733..49806
                     /locus_tag="LP524_000737"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49767..49769,aa:Arg,seq:cct)
     gene            complement(49875..51014)
                     /locus_tag="LP524_000738"
     CDS             complement(49875..51014)
                     /locus_tag="LP524_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL2233811.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(51313..51948)
                     /locus_tag="LP524_000739"
     CDS             complement(51313..51948)
                     /locus_tag="LP524_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233812.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINSASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(52061..52696)
                     /locus_tag="LP524_000740"
     CDS             complement(52061..52696)
                     /locus_tag="LP524_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HBL2233813.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(52730..53191)
                     /locus_tag="LP524_000741"
     CDS             complement(52730..53191)
                     /locus_tag="LP524_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HBL2233814.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(53321..53752)
                     /locus_tag="LP524_000742"
     CDS             complement(53321..53752)
                     /locus_tag="LP524_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBL2233815.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(53770..54090)
                     /locus_tag="LP524_000743"
     CDS             complement(53770..54090)
                     /locus_tag="LP524_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2233816.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            54389..55165
                     /locus_tag="LP524_000744"
     CDS             54389..55165
                     /locus_tag="LP524_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBL2233817.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            55180..55383
                     /locus_tag="LP524_000745"
     CDS             55180..55383
                     /locus_tag="LP524_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2233818.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            55399..55737
                     /locus_tag="LP524_000746"
     CDS             55399..55737
                     /locus_tag="LP524_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HBL2233819.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            55724..55903
                     /locus_tag="LP524_000747"
     CDS             55724..55903
                     /locus_tag="LP524_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233820.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(55946..56416)
                     /locus_tag="LP524_000748"
     CDS             complement(55946..56416)
                     /locus_tag="LP524_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233821.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            56503..57201
                     /locus_tag="LP524_000749"
     CDS             56503..57201
                     /locus_tag="LP524_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HBL2233822.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            57379..57720
                     /locus_tag="LP524_000750"
     CDS             57379..57720
                     /locus_tag="LP524_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233823.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            57713..58384
                     /locus_tag="LP524_000751"
     CDS             57713..58384
                     /locus_tag="LP524_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HBL2233824.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            58390..59076
                     /locus_tag="LP524_000752"
     CDS             58390..59076
                     /locus_tag="LP524_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233825.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            59079..59828
                     /locus_tag="LP524_000753"
     CDS             59079..59828
                     /locus_tag="LP524_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2233826.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            59840..60109
                     /locus_tag="LP524_000754"
     CDS             59840..60109
                     /locus_tag="LP524_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233827.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            60115..60279
                     /locus_tag="LP524_000755"
     CDS             60115..60279
                     /locus_tag="LP524_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233828.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            60272..60571
                     /locus_tag="LP524_000756"
     CDS             60272..60571
                     /locus_tag="LP524_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233829.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            60571..60873
                     /locus_tag="LP524_000757"
     CDS             60571..60873
                     /locus_tag="LP524_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBL2233830.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            60913..61278
                     /locus_tag="LP524_000758"
     CDS             60913..61278
                     /locus_tag="LP524_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233831.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            61768..61908
                     /locus_tag="LP524_000759"
     CDS             61768..61908
                     /locus_tag="LP524_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233832.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            61905..62093
                     /locus_tag="LP524_000760"
     CDS             61905..62093
                     /locus_tag="LP524_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233833.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKNLI"
     gene            62101..62295
                     /locus_tag="LP524_000761"
     CDS             62101..62295
                     /locus_tag="LP524_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233834.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
BASE COUNT        20941 a        10428 c        13099 g        17998 t
ORIGIN      
        1 aataaaagtt gtagtggagg ctacgaagtt tactttgtct ttttacactt aatatttttt
       61 tgccaatttg gaatcaggat gttttgataa ctatcgtttt caattgatgg aagctatatt
      121 gtttgctatc agtgaaataa ttatatggta aacagtgttc tttttcttgt ttccattata
      181 aaaatcgtat cgggattttt cgttctttta aattgaacat ttctatctat ccgcgtgtta
      241 tcaacaaaac aaaatcaggt aaatagttga cgaaacagaa ataaatacat agtattatgt
      301 tttgaagcct aagtacaata gtctatagaa aaggagcgat accattggca aaacgcattg
      361 accgaattta tatgtatgtc aaagaaaaaa ccgctcattt aacaaaggca gaatatgatc
      421 aaggagtaac aacacaggaa attgctgaag tgttagggat tcagcgaaca aatagcagta
      481 aagatctgaa tcagctagtg cgcgaaggaa aactgataaa gacagacgga cgaccagtac
      541 gttacatata tcaaacactc gttacgcacg taaagccagt tacaaaacat gtagtcagtt
      601 acaaagaaac gccggtctct gaagaaaaaa atacactcca aatagatact aaggatatct
      661 ttgcaaaaat cattggagct aacggcagca tgaaaaattc agtggaacag gcaaaagctg
      721 cgattttgta tccgccaaga ggattaaatt gtttgattac aggacctacc ggatcaggaa
      781 agacttattt tgcacacgcg atgtatcatt ttgcaaaagc gcatcatgta atagatgagg
      841 aaacggaact gattgttttc aattgtgctg attatgccaa taaccctgag cttttgatga
      901 gtcatttatt tgggtacgca aaaggtgctt ttacaggcgc agatgaagaa aaaatgggga
      961 ttatcgatca agcggatggc ggtatgctgt ttttggatga gattcaccgg ttgccgcctg
     1021 aaggtcaaga aatgatcttt tactttatgg atcatggaac ttacagtcgt cttggggaaa
     1081 caacgaaatc ccatgaagca aatgtacgga tcgtaggtgc gaccacagaa gacccaggtt
     1141 cttctctatt agaaactttt gttcggcgta tcccaatcaa tatcaaactt ccggcttttg
     1201 aaaaacgtcc tgcaaatgag aaagtcgatt tagtaaaaat catgattgcc cacgaagcaa
     1261 accgcacgca gcgaaagatt tctttgacag aagatgtagc aaaagcattg atcggcagtg
     1321 tgacctatgg aaatatagga caattgaagt caaatatcca gttagtatgt gcacgaggat
     1381 ttctaaatca tatgcaatca cccgaaatct cgatcacaat cgatgatttg acagaaggga
     1441 ttcgtagcgg attgattcaa ttagcaagca accgcagtgc aatggcagag ctttctaaaa
     1501 tattggagcc aaagataact gtagcaccaa atgatacgat catgaaaatc caatcggact
     1561 cctatgagtt accttataat ctatatgata tcatcggaga taaagcagct ttactgaagt
     1621 cggatggttt ggatcaagaa gcaatcaatc atttcatttc tacagatatc aacatccatc
     1681 tgaagtcttt ctataaagac catggttttt cttttaatgc agacagcaaa ttagcagaat
     1741 ttgtagatcc gaaaatcata gaagtgacca atcagattta tgtaatggtc aaaagatctt
     1801 taccatatga gtttcagcaa aactttattt atgctatgag cttgcatatc agttcttttt
     1861 tgaaaaaaat caacttagga gaagaacggc atacgaatga taacatccgt gagatggctt
     1921 ttgattttcc agatgaatat gctgttgcga aagaagtgcg tagttatatc gaaacgtatt
     1981 ttcaagtgga gattccagaa agtgaagact attacttgac cgttctactc gtctcgttac
     2041 gggcaaatca agcaagcggc agaatcggtg tagtcgttgc agctcacgga aacagcacag
     2101 caagcagtat ggtccaagtt gtccaacagt tgttagatgc agagaacgta cgagcagtag
     2161 acatgccttt ggatatggat ccgaaaacag cattggaccg aattgaacgc aatgtacaag
     2221 aagtagatga aggaagcggg gtcatactct tagtagatat ggggtccctt gcgtcgttta
     2281 attcgcaaat tcaaagagac actggaattc cagtacgtac tgttgatatg gtaacaactt
     2341 ctctagtttt ggaaactgta cgaaaggtct cagtattagg gacagatcta gatatgttgc
     2401 atgaatcatt gaaaaatttt cgaggttacg cagaaatatc ttctgtagaa tctgttcagg
     2461 aagcaaaacc tattcataag aaaaaagctg ttttggctat ctgcgcatct ggcgaaggta
     2521 cagcacaaag aatcaaagaa ttgatcgaac gagcggtaaa caagcgtcaa gaaacagaat
     2581 tagaagttct agctttgtcg attgtggaag tgaaagaaga actgccagtt atccaaaaag
     2641 aatatcagat catagctact actgggatta ctgatttgaa tatccaagcc ccatttattc
     2701 ctttagaacg ttttattgat caaaatatcg aagtcatttt ggatcagttg ctgatggaat
     2761 cagaattgga ggaaactgaa ttcatctccc tagatgaaga gtcagctaaa aatacgtgtg
     2821 ttgagtttat ctctgataac ttcatcttta ttaatggtag taagttaatc gatccaatgt
     2881 ggcaattttc tacacaaatc tctcagacga cgggaatcgg cgatgaagaa tatggattca
     2941 aaatcaacct agtgatgcac acagcaggaa tgatcgaacg tattattcgg aatgaaccat
     3001 tgactgtgga agaaaacgaa ttgacaaata caacgaatga tcctctttat agccaattgg
     3061 cagcctcagt tgttctgttg gaagatcaga taaaagtaaa agtgccgata gaagaaatgt
     3121 attatctgct acgtctagtc cataatcagt tagataaaaa agaatacact gtgccatgaa
     3181 tacacaaaaa cgatacactt ttatagtgta tcgtttttgt gtattggtta agaatccttt
     3241 aaattcaatt agtgtatcgt aaaaaagttg gcacgctttt tgcttatagt agtgtgtgct
     3301 aggaggtggt tgttcacaca gaaatgatag gggtttctgt gaagtagtga ggaagaagga
     3361 gttttgcaga tggatattcg attggcacgt atagatgatc gtttaatcca cgggcaagtc
     3421 gcaacagttt ggtcgaagca aacaggtgtt gaacgtatca ttgtgatcag cgatgtagtc
     3481 gctcaagata aattgagaaa gtttctacta aaagaagctg caccgccggg gatcacagca
     3541 aatgtaataa ccgtgcagaa aatgattcaa atctatcata gtggattatt atcacgagca
     3601 aaagtgatgc ttttattcac caatccccaa gatgttgaag cggttgtccg ctgcggtgta
     3661 cagttgaagt cgttgaatat cggcggtatg agttttactg aaggcaaatc aatgattacc
     3721 aattttgttt cagtaaatca acaagatatt gattcctttc gttacttgaa cagtcaagaa
     3781 gtggagttag agattcgaaa agtacctgct gacaggaaaa tccttttgat ggaactacta
     3841 gcgaaaatca aaaaggtgta gcaagaatgg ttgttggaaa aggtcaaata attctgttgt
     3901 aatggttagc aattattaat atttttgaat ttgttacaac aaaagtaaat aggaggtata
     3961 aatatggtag ggattatcct agcaagtcat ggacaatttg ctgaaggaat cttacaatcc
     4021 ggttcaatga tctttggcga acaagaaaac gtcaaagccg tgattttgaa accaagtgaa
     4081 ggtccagatg acttgagagc caaattggaa gaagcggttg cttcttttga taatcaagac
     4141 gaagtgttat tcttagttga tttatggggc ggaacaccgt tcaatcaatc aaacacatta
     4201 ttcgaagagc acaaagataa atgggcaatc gtcagcggtt tgaatttacc aatgttgatt
     4261 gaagcctacg catcacgctt ttcaatggaa tctgctcatg aaattgcagc acacatcatt
     4321 gaaacagcaa aagacggtgt aaaagtaaaa ccggaagaat tagaaccggc agaagcaccg
     4381 aaagcagcag ttgaagacgc acaacctaaa ggagcacttc cagaaggaac tgtggtcgga
     4441 gacggaaaaa tcaaatatgt attagcacgt gttgactcac gtctgttaca tggacaagtt
     4501 gcgacagctt ggacaaaagc agtgcaacca aaccgtatta tcgttgtttc agatgcagta
     4561 tcaaaggatg atctgcgtaa gagactaatc gaacaagctg ctcctccagg tgtaaaagca
     4621 aatgttattc ctattagcaa aatgatcgaa gtagcaaaag accctcgttt tggaaatacc
     4681 aaagcattgc ttttatttga aaatcctgaa gacgtattga cagctgttga aggtggcgta
     4741 gacatcaaag aattaaatgt tgggtcaatg gcgcattcgg tcggtaaagt cgttgtaagt
     4801 aaagtattgt ctatgggaca agaagacgtt gaagcgtttg agaagcttga acaaaaaggt
     4861 gtgaaattcg atgtacgtaa agtacctaat gattcacgcg ataacatgga tgatattttg
     4921 aaaaaagcaa aagctgaatt agcaaaagcg taataaataa tattaaaatt tataggaggc
     4981 ttatcatgtc tattatttca ataattttag tcgtattagt tgcctttcta gctggtatgg
     5041 aaggtattct tgatgaattt caatttcatc aaccactagt ggcatgtaca ttgatcggtt
     5101 tagttaccgg taacctcgaa gctggtatcg tacttggcgg tacgcttcag atgatcgctt
     5161 taggttgggc aaatatcggt gccgctgtag caccagatgc tgcattagca tcagtagcat
     5221 cagcaattat tttagtttta ggtggacaag gtgttaaagg tgttccttct gcgatcgcga
     5281 ttgccgttcc tttggctgtt gcagggttgt tcttaacaat gatcgtacgt acaatcgctg
     5341 ttccaatcgt tcatttgatg gatgcggctg ctgaagaagg aaacatccgt aaggttgaaa
     5401 tgtggcacat cattgctgta tgtttacaag gggttcgtat cgcggtccca gcagcagcgt
     5461 tgctatttat cccagcacaa actgtgcaat cattccttga atcaatgcca gcatggttaa
     5521 cagatggtat ggctatcggt gggggaatgg tcgtagctgt aggttacgct ttggtaatca
     5581 acatgatggc tacaaaagaa gtatggccat tcttcgttat tggtttcgtt gtagctgcaa
     5641 tttctcaatt aacattgatt gctctaggtg ctttaggtgt tgcattagct cttatttact
     5701 tgaacctttc taaaatgggt ggttcatcaa ataatggagg cggaagcaac tctggtgacc
     5761 ctttaggcga cattttgaat gactattaat cttgaaggag gaaaataaaa tggcagaaga
     5821 aaaaatcaaa ttatcaaaaa aagatcgttt agctgttgcg tggcgctcta ccttcattca
     5881 aggttcatgg aactacgaac gtatgcaaaa cggtggctgg gcattttcta tgattccagc
     5941 aatcaaaaaa ttatacaaaa caaaagaaga acgttctgca gcattaaaac gtcacttgga
     6001 attctttaat acacacccat acattgcttc tccaatttta ggggttacat tggctctgga
     6061 agaagaacgt gcgaatggcg cgcctgttga tgatgtagcg attcaagggg ttaaagtagg
     6121 tatgatgggt cctttagctg gtgttggtga cccagtgttc tggtttacag tacgtccaat
     6181 gctaggtgca ctaggtgctt cattagctat gggaggaaat atccttggac caatcatctt
     6241 cttcttaggt tggaacatta ttcgttgggc attcatgtgg tatacacaag aatttggcta
     6301 caaagctggt tctaaaatta ctgatgacct ttctggcgga ttgttacaag acgtaacaaa
     6361 aggtgcttca attttaggga tgttcgtatt agctgccctt gtacaaagat gggtatcaat
     6421 caaattctta ccagttgtat caactgtaaa attagataaa ggtgcttaca tcgaatggga
     6481 taaattgcct tcaggtggtg aaggaatcaa gagtgcattt gaacaagtta ataatggctt
     6541 agccctttct cctgaaaagg taacaacatt acaagacaac ttagaccaat tgattcctgg
     6601 tttagctgca ttaggattaa cattgttatg tatgtggttg cttaagaaaa aggtttctcc
     6661 aatcgttatc atccttggtt tattcgtagt cggtgttgtt ggacacgtca tcggactttt
     6721 ataatccata aaaatatagc tcgggcgttg tcccgagctt tttttatata gtatactgtt
     6781 cataaacaaa tatctggagg gagaaaatgg ttcaatcaat caatacaaaa gtagacttgg
     6841 taattgatgc tacttcgttt acgggtctga cagattacgg gcaaatcatg ataggtgata
     6901 atggttttga gttttaccat gcgcgagata gacgcaaata tatccaaatt ccatgggaag
     6961 aagtggatta tgtactagca tcagtcatgc taaagggaaa atggattcct cgatatgcca
     7021 ttcaaacgaa aaaaaatggt acatacacat tttcttccaa agaacctaaa aaagtgcttc
     7081 gtgcgatcag agaatatgta gatgaagata gaatggtaca atcattgagt ttctttgatg
     7141 tagtgaaacg tggtattcgt agtatgttta aacgcaagta aaaaataaag aaaacttcta
     7201 agaggaggtt ttctttattt ttttactttg cagaagaaaa tatgaaatag caaaaccaat
     7261 tatttgtccc ctttaaatcc catatgctaa aattgattag caaagaagga ggttatatta
     7321 tgagttggtt atggacattg attgttggag ccgtgatcgg agcaattgca ggtgcgatca
     7381 ccagtaaagg aaattcgatg ggctgtgtaa cgaatatcat tgctggacta gtcggttcaa
     7441 gtattggaca agcgattttc ggcgaatggg gaccaaaagc tgcaggtatg gcacttattc
     7501 catctatttt aggcgcagtg attttggtag cagttgtctc atttttcttt ggaaaacgat
     7561 cataagtttt tacaaaatag taaactaaaa ggagttgcag cgagactgtg actccttttt
     7621 ggctttattt atttaaaata aaaagaagtt ttccggaaat ttttatttaa ctgtcgtgta
     7681 cgaagaaaag tttgctataa tggaaaaaag cgtgcaaaga aagagggagt ttcattatga
     7741 tgctacgtgt agaaaaattg cgcaaaaaaa tgcaagaaga aaatttggat tcatttttgg
     7801 ttaccagtcc gtacaatctt cgttacttaa cgaactttac aggtacgaca ggattagctg
     7861 ttattacttt ggaaaaagca ttttttatta ctgattttcg ttataccgag caggcggcag
     7921 cgcaggctca agggtttgaa attattaaaa atgtcggacc tatttttgat gaggtagcaa
     7981 acttagtacg caaagaagaa ctgcactcat tagcttttga ggaaacaaca gtctcatttt
     8041 tagaatattc ggttttagaa gaaatcatcg atgcggaatt agtacctgtt tcacagatga
     8101 tcgaagaact cagagaagtg aaagatgaag aagaaatcgc tattatcgaa aaagcatgtc
     8161 atattgcgga tatggcatat gatcacatcc tgaaaatgat ccaaccgggc atgactgaga
     8221 ttgaagtcgc aaatcaacta gacttttata tgcgttctct tggcgcttca ggtgtttctt
     8281 ttgatacgat cgttgctagc ggattgcgtt cggctatgcc gcatggtgta gcaagcaaaa
     8341 aaatcatcga acaaggtgac ttgatcacat tggactttgg ttgctattac gaagggtacg
     8401 tttccgatat gacacgtaca ttcgctattg gtgatcctgg ggaaaaatta aaagaaattt
     8461 atcaaatagt cttagatgct caattagcag ttattgatgc tgcaaaacca ggaatgactg
     8521 gtgtccaatt agatgcagtt gctagagatt atatttcaaa acatggttac ggggaagcat
     8581 ttggacattc tacaggacat gggatcggtt tagagatcca cgaaggacca aacgtttctt
     8641 ctcgtgcaga aaagcaattt gtaccaggaa atgtgatcac tgatgaacca gggatctact
     8701 taccaggaat cggtggagta cgtattgagg atgacttgtt gatcacagaa gatggaaatc
     8761 gtgtgttgac tcgttcacca aaagaattga tcatcttata gtttcatcaa tagaagaagt
     8821 aagcgtaaaa atcagtgttc acagactttg ttatagaaaa tcactcgatt taatgataca
     8881 ctatagaaga acttttaagc tgtaagagat aataaagttg ggtgttttct ccaattcata
     8941 gcagtttgca gctgatcggc ggcagttttc ttaccacgtg atcactatgc cgcatcatag
     9001 agaattcgga tgtatctcgt gcttaggcaa agggaggaat aaataaatgg cagatgaaaa
     9061 aaatttgatt ttaaacgcaa accaagattt aggagagatc gtgatcgctc ctgaagtcat
     9121 cgaagtaatt atcgggattg cagcttctaa agtagaaggc gtatacggaa tgcgcggaac
     9181 ttttgccagc aatgtcactg aattattagg acgtgcagct cacggaaaag gtgtttactt
     9241 agcaaacgag gaagatggct tgaaagtaga tctttactgt tatctagaat atggtgtttc
     9301 agttcctaaa gtagcaatgg aaatgcagga acgggtcagc cagcaagtcc ttttcatgac
     9361 ggatatcgat ttatctgaag tcaatatcca tgtagtagct gttgttcctg aaaaattaga
     9421 acaaccaaca atcgaagaac tttttgaaga cgaagaggaa gagaatgaat aaagaattat
     9481 caagacacga gattcgggaa atggcattgc aggctttatt tccattagac ttcaatgcag
     9541 acctaacaaa agaagatgcg attttcaacg caatcgaatt agaccaccgc gacatgatca
     9601 acgaagatga atcagagttt gttcctgttt atcttgatac gctagttggc ggtgtctgtg
     9661 caaaaaaaga tgaattggat aaagtgattg aaaaacattt aaaaaacaat tggcacatca
     9721 atcggatctc aaaaatggat ttggtgatct tgcgtatcgc gatttttgaa atgacttatg
     9781 tgacagatgt accagcggct gttgctttaa atgaagcaat cgagttggca aaaacattta
     9841 gtgatgaccg ttcacgtaaa ttcgtcaacg gtgttttatc caatgtattg aaagaattag
     9901 aagctggggc gtaagtccta gcttttttct atgaatcatc ataaacctgt tataaatcgg
     9961 gaaaattcta tggtagaatg aaaacacaaa aaatatctgg gagtggttag atggcagaat
    10021 tgattaacgg aaaagaatta gctgaaaaaa tgcaggcaga gattgctgaa aaaatccaaa
    10081 aattgaaaga taatggtatc catcccggat tagtcgtttt attggtaggg gaaaatccag
    10141 ccagccaaat ctatgtaaga aataaagaac gtgtagcaaa agaaatcgga atccattcat
    10201 tggtagagcg ttatcctgag acgatttctg aagacgaact acttgcagaa attgaaaaat
    10261 acaatcaaga tcctagtttt catggtatat tggtccaatt accattgcca aagcatatag
    10321 atgaagaaaa gatcctgtta gctatccgcc ctgaaaaaga cgtagacggt tttcatccga
    10381 tgaatttagg cagactcctt gcgggtaaac cggacaagat tccttgtact ccatacggca
    10441 tcatgaaaat gtttgaagct tattctatcg atccggcggg taaacgagca gtagttatcg
    10501 gacgaagcaa tatcgtcggt aaaccgatgg ctcagctact cttgatggca gatgcaactg
    10561 ttactgttgc tcattctaaa accgaaaatc tggctgaact ggcacaagaa gcagatattt
    10621 tagtcgtagc aataggacgt ggacattttg tcacaaaaga attcgtaaaa ccaggtgctg
    10681 tagtgataga tgtagggatg aatcgtgatg ctaacggtaa gctgatcggt gatgtgaaat
    10741 ttgatgaagt ggagcctatt gcaggctgga tcactcctgt gccaaagggt gtagggccaa
    10801 tgactatcac gatgctgatg tatcaaaccg taaaatctgc cgaagctttt ggagcaggtg
    10861 aatagatggc acaagaatat ttaactgtca ctacattaac gaagtatttg aaaagaaaat
    10921 ttgatgccga tccttattta gaacgcgtat atttaacagg cgaaatatcg aatttccgtt
    10981 tacgtgccaa tgcccatcaa tatttcagct taaaagatga ccatgcaaaa atatctgcta
    11041 ttatgttcaa aggagcattc caaaaattac gattccagcc aaaagaagga atgaaagtcc
    11101 ttgtcgttgg gcgtatttca ttatatgaag cgggaggttc ttatcaaatc tatgtagagc
    11161 acatggagcc tgatggaata ggtgctttat atcaagaatt ggaagaacga aaagaaaagc
    11221 tgatgaagga aggccttttt caagggccta aaaaaatgct gccacgtttc ccaaagcgaa
    11281 tcgcggtcct taccagtcca agcggagcgg ttatccgcga tatcatcacg acggtgaaaa
    11341 gaagatatcc aattgtccaa ttggttttgt tcccaacagt tgtacaagga aaccaagcag
    11401 cggacaatgt tgtcaggaat atccagagag tagaagagat gggcaatttc gatacaatga
    11461 tcatcggtcg tgggggtggt tcgattgagg atttatggcc gtttaatgaa gagcgggttg
    11521 ctcgggcgat ttatcaagct aatacgccgg tgatttcatc tgtaggacat gaaacagata
    11581 ccacgatcgc tgatttagta gcagatgttc gggctgccac tccaacagca gctgcagaat
    11641 tagctgttcc tgttttgtca gaagagttga tgcgcattca agaacggcaa gctagattgc
    11701 agcaggcgtt tgtacgccag atccagcgca aacaagaacg gttcgagcgt gctcgacgtt
    11761 cctatgtatt tcggcagcct gaaagattgt atgaaggtca gaccatcaaa ttagaccagt
    11821 tgaaacagcg attatatcaa gctacacagc aaatttatca tgcgaaagaa aaacaaacga
    11881 tcgcattaag ccaccaattg gaacaagtgg ctccgcttta tcgagttaaa gcagctcgcc
    11941 aagaaactgc ttatctagaa aaacgcttag aagagaaaat ccaacaatat atgaaacaac
    12001 agcagcagcg ttttgctcaa gctgttcaat ctcttgattt gttgagtcca ttgaaaatca
    12061 tgggaagagg ctacagctat acgacgttaa atgatcaagt agtaaaaagt gtcaccgaga
    12121 ttcatcaagg ggatttgctg acgatccatt atcaagatgg aatcgttaaa ggacaagtac
    12181 aagagataga caaaggagaa taaaaatatg gaaaatccaa cttttgaaga gtcactgcaa
    12241 gaattagaaa aaattgtcat gcagttagaa caaggagacg ttccattaga atcagctcta
    12301 gattcgttta aacgaggaat ggaattaagc aaacattgcc aagatacatt agctaaagca
    12361 gaagagacct tgacgaaaat gatgacagaa tcaaatgaag aagttgattt tgacggaaac
    12421 gaggaaagct gagatgagat taactgactt ttcagctatt catctacctg ctgtagaaaa
    12481 agaaatcttg tcttttttag atgagcatac aacggataga ggactctatg atgcgatgag
    12541 ttattcagtg aaagcaggcg ggaaaagaat acgtccctta ttacttttaa cggccgttgc
    12601 ttcctttgat gagcctattg atgttcccgt ctatcaagta gcagctgctt tagaaatggt
    12661 gcatacgtat tccttgatcc acgatgattt gcctgcgatg gacaatgatg atcttcgtag
    12721 aggaaaacct accaatcata aagtattcgg cgaagcatta gctatattgg ccggagatgg
    12781 tttgcttaca ggagcattcc agctaatcag tatggcacac ttaggtaatt ctcctaagct
    12841 tctgctgctt cagcaattag ctgtttgtgc gggatcacaa ggtatggttg ctgggcaagc
    12901 tgcagatatc gagggcgaat cgaaaaagct ttctttagaa gaattagcat ttattcatga
    12961 aagaaagaca gggcatttga ttcgttatgc cttgctggct ggtggcatat tagcgaagca
    13021 acctgaagaa atcttattgc tacttcaacg attagccgaa catttaggtt tagcttttca
    13081 gataagagat gatctactcg atgtgattgg tacaacgaaa acattaggga aaacagctgg
    13141 taaagatgaa cgtatggaaa aaaatactta cccgcgttta ttaggcctag aaaagacacg
    13201 tgaggcgttg gagattgagc tgttatccgc caacaagatc atagacaaat tagaagagga
    13261 cgttttttct tttgatggcg ggctgatgcg ccagatgatc aagcaatttg atgtggagaa
    13321 atgagggaaa gaatggaaaa agaacgcgta gatgtattag cggtcaaaca aggattattt
    13381 gaaacaagag aacaagccaa acgttcagtg atggctggtt tgatttacaa cgaaaaaaac
    13441 gagcggtttg ataaaccggg agaaaaaatt ccagtgagta gtgaattaag agtcaaaggt
    13501 aaaaaacttc cttatgtttc tcgaggggga ttaaaattag aaaaagcact caaagcattt
    13561 gatctatctg ttgaaggaaa aacattgtta gatattggtg catccactgg cggatttact
    13621 gatgcagctt tgcaaaatgg cgcaaaaatg agttatgctt tagacgttgg ctacaatcag
    13681 ctagcttgga aaataagaca agatcctaga gtcgtcgtaa tggaaagagt gaattttcgt
    13741 tatgcaaaac ccgaagactt taccttagga attccagaag ttgctgttat cgatgtttct
    13801 tttatttcac taaaactgat gctgcctcca ttacatgcta ttttaaaaga agagggagaa
    13861 gtgattgctt taatcaaacc ccaattcgaa gcaggcagag aagctgtagg gaaaaacgga
    13921 attgttcgtg accctcagac ccatcaaaaa gttttagaag acattttgtc atttgcggca
    13981 caaggtggat atgatgtgct ggaattaagt tattctccga tcacgggggg agaaggaaat
    14041 atcgagttct tggctcactt gaggaaagta cccgaaagcg gaacaatcaa ttcagctatt
    14101 aatatggcag aagtggtttc aaatgcacac gaacagtttg accataaata aattgtaact
    14161 gtgaaaatca agtgagaagt atgcggattc ttctttatta taccaaggaa ataagctatc
    14221 attgattaag aagaatatgc taaggagcga agacgtaatg agaaaaaaag atcggcatcg
    14281 tttgattact cgattgttga gtgagcaaga tatccgaaag caggaagaat ttgttgaaat
    14341 cctgaaaagc aaaggaatat ctgttactca agctacgatt tccagagata taaaggaact
    14401 gaaactgatc aaggtaccgg cttcagatgg cggctaccgt tatagcatac cagctgaaac
    14461 aaacgaagat gtaaatacta aattggaaaa actattaaaa gacgctttcg tggcagttga
    14521 ccaaatggaa aaatttgtta ttttgaaaac attgccggga aatgcttcgg cagcagcaaa
    14581 tttgattgat aaacgttaca agaaagaatt attttcgatt atcaatgatg acgataatgt
    14641 tttgatgatt acacgaactg aagaccaggc agttttattg aagaaagact tcctgcatta
    14701 cctttaagtt gaggagcgaa tgtgatgcta caagaaatca gtattactaa ctttgcgatt
    14761 attccggaat tgcggctttc ttttcacgaa ggaatgaccg cgctgaccgg agagactggt
    14821 gcgggtaaat ccattattat cgatgcttta ggtctgttag caggaggaag aggttcaagc
    14881 gattatatac gtcaaggagc agaaaaatgt gtgcttgaag ggttgtttga actaccaaaa
    14941 caagaaggat tctctgaact gatggttgaa ttagggatcg aaacagatga ggacaatctg
    15001 attgttcgac gggatatgtc cttaactgga aaaaatgttt gtcgagtgaa tggtcatatt
    15061 atcacattag ctaatctgag aagaatcggc agttatttag tagatatcca aggacaaaac
    15121 gaacatcagg aactgctcca gcctgaatca catcttgctt tattggatcg ttttggagat
    15181 gtagctttcc aacagaagaa aaagagttat cagcaagaat atatctctta tcgtgagctg
    15241 gaaaaaaggg tacgaaaagt gcagcaaaat gaaaaatcct atgttcagag aatagatatg
    15301 ctccatttcc agcaagaaga aatagctgct gctgatttgc aagtcggaga agaagagaaa
    15361 ttaaaagaag aaagagaaaa acttagcaat tatcaaaaaa ttgctgatgg attagctgca
    15421 ggttacggag cactgacgaa taatgaacaa aacagtgtag atggtgttgg cttggccgtt
    15481 tcagaactgc aagggatcgc ccatctagat gtggagtatg aagcaattta tgaaaatatc
    15541 caaagtgctt actatttgct gcaagatgca atcggcgata tgagtcgtca gattgattta
    15601 ttggagttag atgagagtcg tttagaagaa gttacgcaac gtttagaatt aatccgacag
    15661 ttgaaacgta aatacggcga atccattgag tcaatattgg cttactatga cgaaatcacc
    15721 gaagagctag cttcttctga tttttcagaa ggtcaattgg acaaaatgaa agaggaacta
    15781 gagcaaaaag agcttctact tcagcaacaa gccgcagatc ttcatgaagc gcgtaaaaaa
    15841 atcgcaaaag aattggaaaa atccattctg catgaattga aaagtctgta tatggaaaac
    15901 actgagtttg aggtacgttt cttaaaagaa gaaaatagac aattgaatca tgatggattt
    15961 gaccaaatcg aattttacat tacgaccaat ccaggagagc cactgaagcc tttggtgaaa
    16021 gtcgcttctg gtggcgaact gtctcggatg ttgcttgctt tgaaaactat tttttcttct
    16081 gagcaaggtg tgacaagtat tatttttgat gaagtggata caggtgtgag cggaagagtt
    16141 gcacaggcca tcgcagataa aatttctcaa atatcgaaat actctcaagt tttgtgcatc
    16201 actcatctgc ctcaagttgc tgcagtcgcc gactatcaat attatattgt gaaagcagtg
    16261 attgatggac gtacgcaaac ttctgtttca gagttgaaaa caaaagaaag agaagaagaa
    16321 attgcacgta tgctggctgg aagtgagatt acaaaactca cactcgaaca tgcaaaagaa
    16381 ctattgaaac tcgcaaaaag gacctgagcc acctcgcagt ggttcaggtc cttttcagca
    16441 gctgatagct atattttatt tttacatgac actcattaca gcagtcaata caacagatcc
    16501 aatcgttaaa atcagcattg tccaaatgac aaattttgtt actttagcaa aagtacttgt
    16561 ttgttttttt tcacgcattg ttttcaacct cgtcattaat tagttgattc aagtgtacaa
    16621 tcttttttcc atttccacaa gagggctttt ttataaaaac ttcccttttt ttaaataata
    16681 ggtagttacg atacaaataa ataatgttaa tagaactagc catataaagg catcatccct
    16741 ttcagcaata ggtaacttaa tgttcattcc atagatactg ccgacgattg tcggtatagt
    16801 taaaataatc gttaaagaag ttaaaatttt cataatatta ttcagattat tcgatacgat
    16861 tgctgaaaaa gtctgattca tttgttctac tagttttaat tgaatgcgag aagtagtcat
    16921 tgcctgctct gtttccacca atacatcacg taaatgattt ttcaaagcat gattttcttg
    16981 gaaaagttta ttttgcgcta gttctttcaa tgcatctaaa ttagcagaag tcgcttctct
    17041 gaaataaacg aggctttttt ggatatccat gatttgataa agttgtttgt tttcagtgga
    17101 tacttgaagt ttggcttcca aagtatcgca ttgagattga agcgtagcta aataactgtt
    17161 gtaagagagt gtgacctgcc aaatgaattg aaagaagata ttgagattag gcgaaatatc
    17221 actttttgga aaggtctggc taaagacttt ctttaaaaac agtggttcat gattcgtaat
    17281 tgtgataagc ttctttttgg gcgtcaaaat aatagagagc gggtatgtat ccacttgaaa
    17341 ataaccgcta ggacttgttt tagcaaaagg atattgtaac aataataacg ctggctttgt
    17401 caactttgat tgattgaatt cttctgtacg ggaattttct gcatcgtcca atatggaagt
    17461 gatgtaatct gacggaattt caaattgatg tgtcagctga tgaatctctt cttgattagg
    17521 acgttctaca caataccaga tcgtttggtc agtttccttt ccggtattac gcagtttctc
    17581 attatctaat gtaaaatatt tgatcatgat tttcctcctg ttgcctgttt cttttattat
    17641 aacggatagg gaattggaat acatggaatg tgtttgtaag atatagaata taactgcata
    17701 aatttaaata aaatgaaaca gttgtgtaaa gtatatacag tatacttgaa gcaacgataa
    17761 aaaaggagtt tacgttcatg ggcacattca caccacttat cttgttttgg tatatttacc
    17821 cggtaatcgt tttgtttggt tgccaatttc ttgtaaaatt attttcgtta aatcgtcgtt
    17881 ttaaaataaa agcccctgat ttggcgattc cttttttgtg gggcggtatg catgcattgt
    17941 ctagaaatac agggacaatt tctatcttac cgtttttctt tatttctgtt ttgctaatgg
    18001 gaatttgtat tgctgttttc caagcatatt attatgaaga aattatttat ccgcgttatt
    18061 tcaagatgac ttggcgtttg gtttttctgt tgacaatcgt cttgtacgct gtcttgatca
    18121 ttgtgaacat actttcttac ctatgaaaaa aatagaaaag ctgtaaaaag tcttttaaaa
    18181 ggctttttgc agcttgattt ttaaaaaaat gtttatgaat aaaaaaagtt gttcccggat
    18241 ttcccagaaa aggtggtaga aagtggggga ttgtggtaga ctaattttag ttagtggagg
    18301 aatggggggg ctttcaatgt tgatgggtga attccaacat aatattgatg caaaaggacg
    18361 tttaatcgtg ccctcaaaac ttcgtgaaga acttggcgaa aagtttgttc tgacacgcgg
    18421 attggatggc tgtcttttcg gctatccgat gagcgaatgg gaaaatctgg aagctaaact
    18481 gaacgaaatg ccgttggcta aaaaagacgc acgtactttt gtgcgattct tctattcggc
    18541 tgccactgaa tgcgaacttg ataaacaagg acgaatcaat atacccagca cgttaagaaa
    18601 ttatgcagct ttgacaaaag gttgcgtagt catcggtgta tctaaccgaa ttgaaatttg
    18661 ggatgagaca cgatggcaag atttttctgc agcagcagaa gagaactttg acgaaattgc
    18721 tgaaagtatg attgatttcg gactataaga ataggaagag gctgactaat gactgaaaca
    18781 tttcaacact atacagtcat gttgaaagaa acggtagatg gattgaacat caaaccggat
    18841 ggcgtttatg tagactgtac actcggcggt gccggacata gtgaatattt attaagccaa
    18901 ctaagttcta aagggcatct gtatgcgttt gaccaagatc aaaaagcaat cgatcacgcc
    18961 aagctgcgtt tagcttctta tgtcgaacaa ggtatggtca catttatcaa agcgaacttc
    19021 agagaattag aagaacgctt gagtgagcat gtagataagg tagacggcat tttgtatgat
    19081 ctaggggttt cctctcctca gctagatgaa gcagaacgtg gatttagtta tcatcaagat
    19141 gcaccgttgg atatgcgcat ggatcagtca gctcctttat ccgcttatca cgtaattaat
    19201 gaatattcct accatgaatt agtaaagatt ttcttccgct atggcgagga gaaattttcc
    19261 aaacaaattg ctcgtgaaat cgaacgagta cgaaaaacaa aaccaatcca aacaactggt
    19321 gagcttgtcg aaatcattaa acaagtcatc ccagctcccg caagaagaaa aggcgggcat
    19381 cctgcaaaaa gaatttttca agctgtgcgg attgcagtaa atgacgaact cggtgccgaa
    19441 gaggcctctt tagaacaggc aatccggtta ttgaaagtca atggaagaat cagcgtaatc
    19501 acattccatt cattggaaga cagaattgtc aaaagtatgt ttaaagaata tagtacggta
    19561 caagatctac caccgggact tcctgtcata cctgacgaat tccaaccaga actgaaagtc
    19621 attactcgta aaccaatcct tccttcagag gaagagctaa cagaaaacaa tcgatcacgc
    19681 agtgcaaagt tacgtattgc agaaaaaata agaatgaaag aggagtagaa tgatggcaga
    19741 attaaagaaa atgcaagatt acgaatatga tttacctgaa ttggtggatc agccggaaca
    19801 acaaatgcct gattcttctt cgaatcaaga aatcattctt ccacaatcac cggcaaaacg
    19861 attaaagcag atttctgctt tggaaaaaat tgctgtggca agcgtggcga ttgctattgt
    19921 tgctttatgt gttttaacag ttatgctgcg cacaaatatc agcggagctg aaaaagaaat
    19981 cacgtcaata caaacagaaa tcaatgataa aaagcaagaa aaaacgagtt tagaacaaga
    20041 aaaaaacgaa ctttctcgta ctgaacggat taaacaaatc gcagaagaaa aaggattgtc
    20101 tattaatgac gacaatttaa ggaaagtgaa gtaaatgagt cttaaaaata aaattcggcg
    20161 gtttatggaa aagaaaaatc taaatccgat aaacaatcgc aaaaaagtag gtatcatttt
    20221 atttgctacg agtattggat tgttcttttt atttgccgtc cgtttctcgt atatcgttat
    20281 tggaggtcat gttgctggta cctcattagc tgaaaaaaca aaacagttgt accaaggaag
    20341 tgaagtagta aaagcaaaaa gaggaacgat ttatgatcgt aacggtgtgg cgctcgcaga
    20401 agatgcctcc tcttattcaa ttaaagcgat actctcaaaa acatatacct ctggggataa
    20461 aaaactgtat gttgaagaaa agaattttga taaaatagca gaaatcctgc ataagaatct
    20521 ttctatagac aaaaaagatg cactgaatat tttagaggac ggcgcaaaaa aagagctgta
    20581 tcaagttgaa tttggtagct atgggaaaaa tatctcccaa gaaaccaagc aaaacatcga
    20641 agcggacatg aaaaaagaag gtgtcgctgg tctttatttt gtcgaccatc aggctcggat
    20701 gtatccaaat ggcgtctttt cttctcattt tattggatac gcagttccag acaaagatga
    20761 gaatggctta gttggtaaac taggtttgga gtctgcttac aatgatatct tgagcggtaa
    20821 agatggtaag attatttatc aaaaagataa ttttcaaaat cctctgccag gtacagtggc
    20881 agaagaagaa aaagctgtcg acggacaaga tatctacacg actttagaca gcagactaca
    20941 aagttattta gaaacactga tggatcaagt aaatgaagaa tatcagccag aagaattgac
    21001 agcagtcctg atgaaagcaa aaaccggaga aatcttggca atgggacaac gacctacttt
    21061 caatcctgaa acaatggaag gattgactgg aaaagatgct atctggcgta atttcttggt
    21121 acaagatagt tatgaaccag gatcaaccat gaaagttttc actactgcag ctgctatcga
    21181 agaaggagaa ttcaacgaga atgaaacctt ccaatctgga aaaatccaag tagaagatgc
    21241 tacgatcaat gaccatgact ttggtgaaaa aggtgtgctg accatgcgtc aagctctttc
    21301 atggtcaagt aacgtcggga tggtcatact ggaacagcga ttaggcggac gatggtataa
    21361 ctatttacaa aaactgggat ttggacaaag tactcattct ggattagatg atgaagtaaa
    21421 cggtgcttta cctacattaa atattgtcga ccgagcaatg agtgcttatg gacaagcggt
    21481 aggtgtgacg aatttccaaa tgatgaaagc tttcacttct attgcgaata acggtacaat
    21541 gattcaacct cgatatatca gcaaagtggt ggatccccaa actggagaag agcgaacaac
    21601 tcaaacagaa gttctagggc aacctttttc aaaagaaaca acagaaaaag tccgtgaata
    21661 tatgagagat gttgtcgaaa gcgaaaacta cggtagtgct tatggtgtat atagtgtacc
    21721 tggatacaat gtatcggcaa aaaccggaac agcccaaatc gcttctgata cagggggata
    21781 tcaaacagga gatacagcct acctgtattc gattgtggag atggttcctt ctgaagatcc
    21841 agactatgtc ttgtatctaa caatgaaaca ccctaagaca tatgatcgaa tggcattggc
    21901 taaaatcgca aatccattga tgaaacgggc aatggatttt aaagaaactg aagaagattc
    21961 agatacagaa acaaaaacag aaaaagtatc cgtagccgat tatcgtaatc ttgaagcgga
    22021 cgttgcagca gctgatgctc aaaaaagtgg actgcaacct gtagtgatcg gtaatggtaa
    22081 aaaagtacaa aaacaatcta ctgccaacgg tgatcagttg atctctggag aaaaactgat
    22141 cctatatact ggtggtgata agctgatgcc tgacgtaaca ggatggtcaa aagcagatat
    22201 catgaagctg gggaaaatcc tcggtattga agttagtttt gatggagatg gttattgtgt
    22261 caaacaagaa cttgctccat atgaaaaaat cactgaagac aaacttagtt ttactttaga
    22321 agaataagaa gagaaatagg agagaaaaga gcatggaatg gacacaagca ttgattccca
    22381 ttgtcagcag ttgtgcaatg acaattgccg caatgccgct atttattggt tattttcaaa
    22441 tgaaaaagca aggtcaggcg attcgtgagg agggaccaaa gtggcacaat agcaaagccg
    22501 gaactcctac aatgggcggc ttagtgtttc tgatcggctc tattttgaca ggaatttggg
    22561 ttggagcttg gcaaaaacag ctaactccta ctcttttcat cttgcttttt gtattagcgt
    22621 tatacggagt aatcggcttt cttgatgatt ttataaaaat attcaagaaa agaaacatgg
    22681 gactgaactc gaagcagaaa ttgattggac agattctagg gggaatcatc ttttatcttg
    22741 tttaccgttc tgaaggttat ccaggtgtat tgaatttctt cggactagat ctacctttag
    22801 gaatcattta tggtattttc gctctcttct ggcttgttgg attttcaaat gcagtaaatc
    22861 tgacagatgg catcgatgga ttagttgctg gcttaggaag tatctctttt gcagcttacg
    22921 gtctaattgc ttggcatcag catcagtatg atgtattaat catttgctta agtgttttag
    22981 gaggtttact gggtttcttc gtctacaaca gaaaacctgc caagattttt atgggagatg
    23041 tcggttcatt agcgttgggc ggacttttag cggcaatttc tataatgcta aaccaagaat
    23101 ggacattgct tttgattggt ttgatctatg ttatggaaac agcaagtgtg atgcttcaag
    23161 ttacttcctt taagcttaca ggaaaacgaa tttttaaaat gtctccaatt catcatcact
    23221 ttgaaatgtg cggatggtcg gagtggaaga ttgatacagt tttttggttg atcagcattg
    23281 tgacttcgtt gatcacatta tggatcgttt ggtaggagat gacgggaaat gaaaaaaata
    23341 tcaacatatg aaaataagaa agtgcttgtc ttaggactcg caaaaagcgg agtaagtgcg
    23401 gccaaattat tacatgaatt gggcgcactt gtcaccgtta atgatggaaa accttttgac
    23461 gaaaatccgg aagcacaaga gctgctttct ttagggataa aagtaataac aggtagtcat
    23521 ccaatcgaat tgttggatga agaattttca ttgatggtca aaaatccagg gattccctac
    23581 agtcatcctt tagtagcaaa agcccaagaa atgggtattc cggtaattac ggaagttgag
    23641 ctagcttacg aagtagcgga atgcccaatt attgggatta caggaacgaa cggaaaaaca
    23701 acgacaacaa cgatgacagg actactactg aatgcaggag cagatcaagg aattgcacgt
    23761 ttagcgggta atattgggta tccagcaagt ggtgtagcgc aagaagcaaa atcagaagat
    23821 aaaattgtga tggaattgtc tagcttccaa ctcatgggaa tcacagattt ccgccctcat
    23881 atagcggtga ttaccaatat ctatgaagca catattgatt atcatggtac aagaaaagaa
    23941 tatgtcaaag ctaaatggaa ccttcaaaaa aacatgacag aaaaagatta tctgatcctt
    24001 aactggaatc agtcagaact acaagaatta gcgcaaagaa caaaagcacg cgtactgcct
    24061 ttctcgacaa aagaagtgtt ggaagacggt gtgtatgctg acgattactc aatatactat
    24121 aaaaaagaaa aaataatgga aatatctgaa cttggcgtac caggtaagca taatgtagaa
    24181 aatgcactag ctgctatttc tgtagcaaaa ttgtatggta tatcaaatga agctatacga
    24241 gaaacgttgc attttttcca tggcgtacca catcgtacgc aatatgtagg agaaatccaa
    24301 ggcagaaaat tttataatga ttcaaaagca accaatattt tagcgactaa aatggctcta
    24361 agtggatttg aaacgtctaa ggtcgtgtta ttggctggcg gattagacag aggaaataca
    24421 tttgatgaat taattccttc attaaaagga atcaaagcaa tggtcgtatt tggccaaaca
    24481 aaagaaaagt tgatggatgc tggaaaaaaa gcaggcatcg agacaatagt aacagccgat
    24541 tctgtagaac aagcagttcc tttagcatta gaaaacagta cagatggtga tgtagtttta
    24601 ttatcaccag cgaatgccag ctgggatcaa tacccaaatt ttgaaactag aggaaacaga
    24661 ttcatggaag ctgtgaaccg attaaaatag aaaaagtaga agcgagcgat aaaaaatgaa
    24721 aattttggtt acaggcggag gaacaggggg acatatttat ccagctcttg cctttgtcaa
    24781 ttatgtaaag actcaagaac cgaatgctga attcatgtat gtgggagcaa aacgaggtct
    24841 agaaaacaaa atcgtccctg atacaggaat gcctttccat accttggaga ttcaaggatt
    24901 taaacgaaaa atttccatgc ataacatcaa gacggtccga ttgtttctta agagtatccg
    24961 tcaagcaaaa aaaatcctca gtgattttca accagatatc gttatcggta ctggaggata
    25021 tgtttccgga gcggtagtgt acgctgcagc caaaatgggt attccaacag ttatccatga
    25081 acaaaacagt gtaccaggtg ttaccaataa atttttaagc cgatatgtgg atcggatcgc
    25141 tctttcattt aaagatgcgg cttccttttt tcctgaaaac aaagcagttc ttgtaggtaa
    25201 tcctcgagca caagaagtag cagatactaa aaaatcagat attttgaaaa catttgattt
    25261 ggatccagaa aagaaaacag tcttgatttt tggcggtagt caaggagcac ttaaaatcaa
    25321 tcaagcagta tcagtttttt taagtgaata tattccttct tcttaccaag ttttgtatgc
    25381 ttcaggagat agatattatg aagagatcct aaataaaata aatcctgaaa tacaaaatat
    25441 tagtgtccag ccttatatca aaaatatggc agaggtcatg gcttccagtg atctgctggt
    25501 ggggcgtgct ggtgcaactt cgatcgcaga attcacagct ctggggttac cggcagtgtt
    25561 ggttcccagc ccatatgtta cgaatgatca tcagactaaa aatgcaatga gtcttgttca
    25621 cgcaggagca gcaaaaatga ttgcagataa tgaactaaca ggtgaatcac taagtcagac
    25681 agtcaacgaa atcatgggtg atgaagaatt gcaaaagcaa atgtgccgag cttctaaaga
    25741 acaaggaatc cctgatgctt caaaacgtct ttatgactta gtaaaacaaa taatcaaaaa
    25801 ataaaggcgg tggtcttagt gagtaagaaa aaagaaccaa aaaaattgtc tcatccgtct
    25861 tctgatgaaa acaatttgac tccttggcaa aaggctaacc gaaaatatct ggcagaacaa
    25921 gagatgaaaa accaagagcc aaaaaaagaa gacataacat tagatacacc cctttcagat
    25981 tctgaagaag aaaaaacggt tcaagaggaa cagcaagaag aaagccagtc cgagaaaaaa
    26041 atcgaatttt ttgaagagat caagcaaaag aaaaacggtg gaccatataa cggttctttt
    26101 ttgaaccggc tgcccaattt aaaaagtcaa cggcacaaag tactatttcg tcggttagct
    26161 ttgatcatca ctgtattggg tataccgctg atttttttga tctattatgt ttctccttat
    26221 agcaaattac aagcagttgc agtatcaggc aataagacag tcaacagcca agaaatcatc
    26281 tctgatacaa aattgtcatt aggagaaaat gtttggggac agtattttca tcgatctact
    26341 tatatcgatc gccttaaaaa agcacagcca agaatcgaaa cagcaaatat acatttcaaa
    26401 gggatgaatg aattcgattt ggatgtaaca gaatataaag agattgcttt gatcgcaaaa
    26461 aacaatcaat actacccagt gattgaaaat ggaacagtat tagatgaaaa agtagcaaat
    26521 cctacaaaaa atctccctat cttggaagag ttcaaagact ctgcaaaaat caaagaatta
    26581 acaaagcaat acaatcagtt atcatcagaa ttacaaaaag caatttctga aatcaagtac
    26641 acgccaagag caagcaataa aaatctgatc caattgaata tgaatgacgg caatcaagtt
    26701 attgtaaata tcaataatct tgcgaaccaa atgaaatatt attcgcaagt agccaaagat
    26761 atggatgaaa aaggtgtgat cgacatggaa gttggtatct tctcgcatcc ttacggtagc
    26821 actcaagaaa cggaagaatc gggccaatcg gaagaaacaa ctgaaagtga agaaaatgcg
    26881 gatcaaacaa acgaatcaag tgaagaggca gaaacgcaaa aagcacaaac acctggatcg
    26941 tctagcacaa acggatcatc tgattcaaat gattcaagtg aaacaagtca aatagaagat
    27001 aatccaagta caactccgtc aagtttaaat gaaaattgat aaaacaactt taaaaagtaa
    27061 gaatgtactt tctaaaaacc agtaatttat ggtagaattg gaagcagtga atatatctat
    27121 taataagtat attttaaaac ggacgctagt tgttcgcttg tatatggaat agtaagtagg
    27181 agggaatccc attcatggca aaaacaggaa tgtatgtagg ccttgatatt ggaacgacat
    27241 ctgttaaagt tgtcgtggct gaatatatcg acagccaaat gaatattatt ggagtaggaa
    27301 acgcaaaatc agaagggatt aaccgaggca ttatcgttga tatcgataaa acggtccaag
    27361 cgatacaacg agcagtacga caagcagaag aaaaagcagg tatccagatc aaaggcgtaa
    27421 gtgtcggttt gccagcaaac atgcttgaag tagaaaattg ccagggaatg attgcagtta
    27481 acggagattc aaaggaaatc accgatgaag atgtcagaaa tgtggcttca gctgcccttg
    27541 ttcgttcaat acctcctgaa cgtcaaatcg tgtcaatatt gccacaagat tttactgtcg
    27601 atgggttcga gggaatcaaa gatcctcgtg gaatgatcgg cgtaagatta gaaatgtacg
    27661 gtctgttgtt tacaggacct aaaacgatca tccataacat acgtaaatgc gtggaaaatg
    27721 ccggattaat cgtcaacgaa atggtgatta ctcctttagc gctgactgaa tcaattcttt
    27781 ctgacggaga aaaagatttt ggtacgatcg tcattgatat gggtggcggc caaacaacaa
    27841 cggctgttat gcatgataag caattgaagt ttaccaatct tgaccaagaa ggtggcgaat
    27901 ttgtcactaa agatatctct attgtattaa atacgtcatt taacaatgca gaagcgttga
    27961 aaataaacta cggggatgca tacccagaac ggacatcacc agatgaagaa tttccagtag
    28021 atgtgattgg tcagtcagaa ccagtgaaag tcgatgaacg ctacttgtca gaaattattt
    28081 cagctcgaat ggaacagatt ttcaacaaag caaaagatgc attggaccaa atcgaagcat
    28141 tagagttgcc aggtggcgtc gtattgacag gcggagctgc tagtcttcct ggtgttgtgg
    28201 acttggcaca agaaatcttc ggcgtaaatg taaaattgta tgttccaaat cacatggggc
    28261 tgagaaaccc ggtatttaca aatgtaataa gtatcgtgga ttattcagca aacttgagtg
    28321 aagtgtatca attggcaaaa attgctgtta cgggtgaaac ctcagcagct cgtcaaatgg
    28381 tagttgaaca agaaacaaca aacacatatg agagctatga agcacctgaa gaaacaatct
    28441 acgatgagcc ggaaccaaaa gagtccggag aaaatgtaaa aaataaaatc aagggtttct
    28501 ttacaaatat ttttgattaa ttcatcctgg gaggaataag atatggaatt ctcaattgat
    28561 aataatatca atgacggcgc agtaatcaaa gtcatcggag tcggtggtgg cggtggaaat
    28621 gctgtaaacc gaatgattga agaaaacgtt aaaggtgttg aattcattac agccaacaca
    28681 gacgtgcaag cattaaaaaa ttcaaaagct gaaacagtta tccaattagg ccccaaatat
    28741 actcgtggtt taggtgctgg ttctcagcca gaagttggac aaaaagcggc agaagaaagt
    28801 gaacaatcct tacgcgaagc tttagacggt gcagatatga ttttcatcac tgcaggaatg
    28861 ggtggaggaa ccggtacagg tgctgcacca atcgttgcag gtatcgcgaa agaattgggc
    28921 gcattgactg ttggtgtcgt gacacgtcca ttcacttttg aaggaccgaa acgtggccgt
    28981 tttgctgcag aaggtatcgc tcgtttgaaa gaaaatgtgg atacattatt aattatctca
    29041 aataaccgtt tattagaagt tgtagataaa aagacaccga tgcttgaagc tttccgtgaa
    29101 gcagataatg ttttacgaca aggtgtacaa ggtatctctg atttgatcac agcgccaggt
    29161 tacgttaacc ttgactttgc tgacgtgaaa acagtgatgg aaaaccaagg tacagcattg
    29221 atggggatcg gtgtggctag tggagaagat cgcgtagtag aagcaacgaa aaaagcaatc
    29281 tcctctccat tgcttgaaac atctattgat ggagctgaac aagtacttct taacatcaca
    29341 ggcggtttgg atatgacatt gttcgaagca caagatgctt cagatatcgt agcaaatgca
    29401 gcaactggcg atgtaaatat cattctaggt acatctatca atgaagaaat gggcgatgaa
    29461 atccgtgtga cggtcatcgc aacaggtatc gatgaatcaa aaaaggaacg caagtctagc
    29521 cgaccagcta gacaagcaca aatgcaatca cctgctcaaa agactgtatt agatatggat
    29581 caagctaaac caacttcttc agaagaagaa aacagttttg gcgattggga tatcagacgt
    29641 gaacaaaatg ttcgtcctag agttgatgat tccaattttg acaatatcga aaaaaaagaa
    29701 tttgatacat tcaaccgcga agaaacaaaa tctcatggcg atgatgaatt aaatacgcca
    29761 ccgtttttcc gtcgtaaaaa ataaaggaga ggaagagcac atgattgctg acaacttgca
    29821 gaaaatcaag caagaaattc aggattcgtg tgctcttgtt catcgtgatc cgaaagaagt
    29881 tacattagta gctgtaacaa aatctgttga atccgctcaa gctgaagaat tggttagagc
    29941 tggcgttatg aattgtgcag aaaaccgagt agataaatta ttagaaaaga aacaagattt
    30001 atttgatttt cctgaaataa agtggcattt aattggtaat ttacagcgca gaaaggtaaa
    30061 attgattgta aatgaaattg attactttca tgcgttggac agtttaagat tagcagaaga
    30121 aatccagaag cggttagaga aagaattatc ctgttttatt gaagtaaatg tttctggtga
    30181 ggaatcaaaa catggaattt caccagatga gcttttaccg tttgtggaat ctttagctgc
    30241 ctgcacaaaa atcaaggttg ttgggttgat gactatggcg cctaaagaag cttctgatga
    30301 gcaaatccat aaagtgtttg gtacgctgaa agaattgcgt gatcttgttc aagcaaaagg
    30361 ttatgcccat gcaccatgta gtgaattaag tatggggatg agtcaagact ttcaaattgc
    30421 gattgaagaa ggtgcaacat ttatccgtgt aggaacagcg ctattcaaag acgaataaag
    30481 gaggaacaaa aatgccgctt ttaaacagag gagcaattgc taattttttc ggattagctg
    30541 atgaggaaga gtacgaatac caagattatc ctgaaacaac aaaaaaggct gtacagccaa
    30601 ctaaaaaaaa tacaacacag aatcaatcaa ctcattctgt atctgctgcg aatactcatg
    30661 aaaaacaaaa aacggcgact tcacgtcctg taacgcatga aagtattcag ccggctgaaa
    30721 agaaaaaagt aattgaacta catcaatcac ctacaaaaac tgtagcgaaa gaaagaacag
    30781 cttcaagaac ctctacacaa cctaaagcaa atcttgctgg aaaaatcact gtaatggaac
    30841 caagagctta ttctgaagct atggcgattg ctaaacgaat cattgctgga gaagcaacct
    30901 tagtgaattt tcatttagta gaagaaaaac aagcgagaag aatcgttgat tttctaacag
    30961 gaacagttta tgcactagat ggagatatca aacgtgttgg agatgagatt tttctttgta
    31021 caccgactgg gatggagatt gacagttcaa ccgctcaatc ttttgcagat gcgaatttgt
    31081 ttgatctgta agaggaggaa ctacgattta tactattgca ctattacttc atttacttca
    31141 acgggcagcc tatttctata gcattatctt gatggtatat gcattgctct cttggtttcc
    31201 aggcgggtat caatcagggc taggacgatt attagctaag atctgcgaac cgtatatcag
    31261 tttgttcgat cgtctgcctt tgaggattgg acccttggat ttcacaattt taggggcaat
    31321 cctgatcttg aacttagccg tacaaggttt agcacagatc gttatatgga taactgtcca
    31381 tttagtttat tgattgaagc aacaaaatct ttattctcag aatagatagg aggggaaaat
    31441 tttggatgca aatgtatacc agcactttcg ggcagatgaa cgtacattta tcgattcggt
    31501 aggcgattgg attgaacagg ttcaaggaca atatgcaccc tatctgaccg aatttcttga
    31561 cccaagacaa tcctatattt tagaaacact gattcgacaa agcagtgatt tgcgtttcat
    31621 gttttatggc ggatatgaac aggcagaaag aaaaagatgt ttgattttcc ccgattatta
    31681 cgaacctgta gaggaagatt ttgaaacagc attgtatgaa atccattatc ctagcaaatt
    31741 tgccagccta agtcatggga aaatccttgg aactttagtg ggaacgggga tcaaacgctc
    31801 ttactttggc gatatcatct cggacggtga tcgctggcaa gtatttattg caaatgaggt
    31861 ggatcatttt gttgctgcac aagtaacaaa gattggaaag gttgctgtac gcttggaacc
    31921 aatcaaatac acagaaatga ttatgccgaa agatagctgg acacaagaaa gaggaacagt
    31981 tagttccatg cgactagaca gtgtgatctc tgagatttat aatatttcca gacagcggtc
    32041 caagcagctg atcgaagctg gtaaagtaaa agtgaactgg acagaaaata caagacctga
    32101 ttttgtactt gaactattag atattatttc agtcagaggt tatggcagag tccagatcca
    32161 agaacttgag gggaagacga aaaaagaaaa gtacagggta ctgttcggtg tattacgtaa
    32221 ataaaataaa aaggtggttt actcatggct ttaactccat tagatatcca aaataagaca
    32281 ttcccgacaa aaatgcgggg atataatcaa gacgaagtag atgatttctt agatttagtt
    32341 gttcgtgact atgaggaatt gactcaaaga aatcgagaat tagaaaaagc agtcaaacat
    32401 tctgaagaaa aacttgagta cttcaatgaa ttaaaagatg ctttgaacca atcaatcatc
    32461 gtagctcaag atacggctga taaagtaaaa acaagtgcaa gcaaggaatc cgaagtgatc
    32521 gttacttctg cacaaaacaa agcagatgag ttagttgcga acgctgaaaa acgtgcacat
    32581 caattaacaa ctgatgcaga agaaaaagca agaaaaatct tgacagatgc aacagaaaaa
    32641 gcaagacagc ttgcgacaga aactgaagat cttaagaaaa aaacacgagt attccatcaa
    32701 cgtatcagct tgatgcttga gtctcaatta gaacaagtga aaagtccgga atgggatgaa
    32761 attttacaac cattctccag ctacgtgact gacagccatg aagtgatcaa agaagtttta
    32821 gcaaaacagc ttgacaatga aaatgatact gatgtaaact cagacacagg agtcatagaa
    32881 gcacctatga cggcatttga tacacaggct attgatatct cgatcgtgcc tggtgaaaat
    32941 gaaaacgagt agaacagaaa agcactttgt tattcttaaa gcgagccgat gagagtggaa
    33001 gttcggtaag acactgcatg gtgaaagatc ctctacttga gacctgcttt tccgaaatta
    33061 gagtaagaag cagcggctga cctcgttatt ggtcacagag agtttgcact agatagatgc
    33121 aactaaattt gggtggtacc acggcatgtt cgtcccttgc ggctgaacat gcttttttgt
    33181 gtccggaaaa caaataaagg agtgtatggt tgaatgaaaa tgaaagaaac cttgcaatta
    33241 ggaaaaacaa aattccctat gcgtggtaat ttgccaaacc gagaaggcga gtggcaaaaa
    33301 gaatgggaag aaaatcgaat ctaccaaaaa agacaagagt tgaacgaagg aaaacctagc
    33361 tttgttcttc atgatggacc tccatatgcc aatggtaata tccatttagg acattcattg
    33421 aataaaatca gtaaagacat tatcattcgt tcaaaatcaa tgtctggcta tcgtgcacca
    33481 tatgttccag gatgggatac acatggattg ccgattgaac aagtattggc taataaaggc
    33541 gtgaaacgta aagaaatgtc catggcagaa tacttggaaa aatgtcgtga atatgcactt
    33601 tcccaagtag ataagcaacg agcagatttc aaacgcctag gagttgcagg agattgggaa
    33661 catccttatg ttacattaga tccttcttat gaagctgcac aagtacgcgt atttggtaaa
    33721 atggctgaaa aaggctatat ttacaaagga ttgaaaccaa tttactggtc tccgtcaagt
    33781 gagtcttctt tagcggaagc tgaaattgaa tataaagatg taaaatctcc atctattttt
    33841 gtagctttca atgtcaaaga cggcaaagat attcttgatg aagatacagc ttttgtcatt
    33901 tggacgacaa caccatggac attgccagca aaccaaggta ttgctgtaaa tccaaaattt
    33961 acgtatgtat tggtggaagc cgatggccgt aagtttgtcg tagcgaaaga tttgctagaa
    34021 acagtccaat cagaaatcgg ttgggaaaat gtaacagttc taaaagaatt tgctggacaa
    34081 gaaatggaat atatgactgc aagtcatcca ttctatgaac gtgaatcact tgtgatttta
    34141 ggcgatcatg ttactttaga cgctggtact ggattagtcc atactgctcc aggacatggg
    34201 gaagatgact atatcgctgg tagcaaatat aaactatctg tagtgtcacc tgtagactca
    34261 aaaggattgt ttacagaaga agcacctgga tttgaaggta tcttttatga caaagcaaat
    34321 cctatgatca ctgatctatt aaaagaaaaa ggagctttgc tgaagctaga tttctttact
    34381 catagttatc ctcacgattg gcggacgaaa aagcctgtga tctatcgggc aactccacaa
    34441 tggtttgcat ctatcgataa attccgtcag aatattttgg atgaagtaga aaaagtagag
    34501 tgggttatcc catggggtaa aacacgtttg tataatatga tccgtgaccg cggcgattgg
    34561 gtaatttccc gtcagcgtgc atggggagta ccacttccga ttttctatgc tgaaaatgga
    34621 gaagcaatca tcacaccaga aacaatcgaa catgtggcaa aattatttgc tgaacatgga
    34681 tcaattatct ggtttgaaaa agaagcgaaa gaacttcttc ctgaaggatt tacccaccca
    34741 ggttctccta atggcgaatt tacaaaagaa aaagatatca tggatgtctg gtttgattct
    34801 ggttcttctc atgaagctgt tttacgtcag cgaccagaat tatcattccc agcagatatg
    34861 tatttagaag gatcagacca atatcgtggc tggttcaact caagtatcac gactagtgtg
    34921 gctatcaatg gtgtagcacc atacaaatca gttatttctc aaggatttgc tttagatggt
    34981 gaaggccgta agatgagtaa atctttagga aacatcattg caccagaaaa agtgatcaaa
    35041 caatttggtg cagatatcct tcgcttatgg gtatctagtg tagattatga agcagatgtt
    35101 cgtgtttcta tggatatctt aagtcaagtg gcagaagtat atcgtaaaat cagaaatacg
    35161 atgcgtttct tattagcgaa tacagaagat ttcgatccta aaaaagatac tgtttcgtac
    35221 aatgatttac gttctgtaga caaatatatg accgttcgat tgaaccaagt aatcaaagaa
    35281 atccgagaag aagggtacga caaatataac ttcatgcata tttaccgtac agttatgaac
    35341 ttcttgacgg ttgatctatc gtcattctac ctggattttg ctaaagatgt agtttatatt
    35401 gaagcagaag atgattatca acgtcgttgc atgcaaactg ttttctatca aacagctgta
    35461 gctttgacaa aattattgac tcctatcatt ccgcatacag cagaagaaat ctggtcattc
    35521 ctgaaagaag aagaggagta cgttcagtta agcgagtttc ctggttatga agagtttgca
    35581 aaccaagaag aattgatgga tacctggact gctttcatgg acttccgtga taatgtctta
    35641 aaagcattgg aagaagcaag aaacagcaaa ttgattggta aatctttgga agcaaaacta
    35701 actgtttatc caaaacaaca agttcgtgaa atgttaaaag ctttggatgc agatattgca
    35761 caattattga tcgtttcacc agactacttt gaagttatgg cagcagatga acaagtgcca
    35821 gataacgcta tggtatttga tgatgttgct attacagtgg aaaaagcaga cggggaaact
    35881 tgcgatcgct gtcgccaagt tcgtaaagat gtaggagtag atgaaaaact tcctcatcta
    35941 tgcggtcgtt gtgcaaaaat cgttgaagac ttctatcctg aagctgtagc tgaaggattc
    36001 gaagaaaagt aaaagggaaa agggggtgtg acatgttctt tgtcacaccc ccttttgtta
    36061 tcaatcgttt aattttccac gttcacgata ccagttatct ggttgccaga tccattcgaa
    36121 tccgtctttt tcaagtagtt cgaaagctgc tttaggtccc attgatccag ctttgtagtt
    36181 gggaaaatcc ggttgtgttt ggtcccaagc ttttcgaatg acatccacga tatgccaaga
    36241 ttgagcgact tcttcccaat gagagaagtt cgttccatca ccattcaacg catctaatag
    36301 taatttttcg taagcttctg ggctattttc aaccatttct gcactatttc taaattccaa
    36361 tttgattggt tccgtttcaa atccttgccc tacttctttc ccattcaagg ataaagaaaa
    36421 tccttcagtt ggctgaatat agatcgttag aacattaggt ggaagtgtcg tatcgtcaca
    36481 tggttcatcg acagatgttt tgaatacatt aacaggaact tgtttgaata cgatgttgat
    36541 ccgagtccct ttttcagtca aacgtttgcc tgtacgtaca tagaaaggaa cacctgacca
    36601 acggaagtta tcgatagtga atttgcctgc gacgaatgtt tcagtctctg attgagcatc
    36661 cactttgtct tcttcgcgat agcccttgaa ttgtacacca tccaattcac cttgagcata
    36721 ttgcccgcga acgaagtttt ctaacacttc ttcttcgtta taaatgcgga tagctttcaa
    36781 tgctttgatc ttttctgtac ggatttcttt ttcggaaaat gctactggcg gttccattgc
    36841 aagtaatgct acaacctgta aaatatgatt ttgcaccatg tctttaagtg cgccactatg
    36901 atcgtagtag cccccgcgat cttctacacc gaggttttcg ccaaaagtaa tttggatatt
    36961 gtcgatatag cgattgttcc attgagcctc aaaaatatta ttggcaaaac ggatagctga
    37021 gatgttttgg atcatttctt ttcctaaata atgatcaatc ctgaaaatat cttgttctgg
    37081 gaatacttct ctgatttgct ggttcaattc aaaagcagac tggtaatcag aaccgaacgg
    37141 tttttctatg atcaaacgat tgaagccatt ttttgtcata atattttgtg atttcaaatg
    37201 agaaacgatc gttccaaaaa attgaggtgc catagccaaa taatagacac ggttaccgtt
    37261 taattgatag tgtccatcta atcggtcaga caattctttt aatgtctgat aatgcgttga
    37321 atcatttaca tcatgggatt gatagtaaaa atggcttgca aaggtagttg cttcttcatc
    37381 tgaaggattc aatgcacgga ttgtttcttt gacaacttca cggtagtgct catcgctcca
    37441 gggacggcga gcggttccga ttacagcaaa atgctcgcct aaatttcctt ttctgtaaag
    37501 gcggaacaaa gacgggtaca atttacgctg ggctaggtca cctgtaccgc cgaatatcgt
    37561 aaacaataca tttttttcat tagacatgtg gatacactcc tttatatttt atcgacaaaa
    37621 atcgtgctgg ccgccttaaa gctgacgggg attttctttc tatcattttg gatagtgatc
    37681 ggtccttcat aagctgcgat gtcaacgatc tcatattctt cgttgatatt caggtcaata
    37741 gtgactaagt aatccaacaa ttctttttca tcaagcactc gtgcgatacg cacttttgta
    37801 ccgacaggat aggactccaa tgtttgtcgg cgttcttcat ggacaacttc ctctttttta
    37861 ggaatcgatc ctccatgagg acaatgttca ggatgattca aatagttttc taaatgatct
    37921 gcaagatgtt ccgaagtcac atgttccaat acttcagcat catcatggac ttcattccag
    37981 ctatagtcta aatgttcaac taaaaaaact tcccataagc gatgtttgcg aatcagactg
    38041 ctagcttttt gcagtccgct tgcagtcaat tgtacacctt gataagggga atgttctaca
    38101 agctcttctt tcacaagttt agaaatcatt tcactgacag atgcagctga gacatctaga
    38161 ctagatacga tttgtttatt gttgactttc gttgtatctc cacctagttc taaaatgatt
    38221 ttcaaataat cttctcgatt tggtgtcata ttcgatcatc ctttcatggt aaaaaaccat
    38281 cattagtaag tgtaacaaaa aaaacgttaa ttgactattt ttctaaataa aaaacaaagg
    38341 atttttagac gtttttacaa caaaaaacga aagatgatag agaagactca ttctttcgtt
    38401 tataaaggat atatatatac attgggaggg atctattttt cagataacaa cttatatgaa
    38461 gtgtctgcag attcgtcata aagagtttgt tcgggataag aaaagataag ataaaaacca
    38521 tactgatcac gtacgactaa acgatattca tctatttcta ccacagatgt gccaagagac
    38581 tgaccgttga tcaaaaacgt gtaatcagat gtaatctgta attgttgttt ttgctggcta
    38641 tcactaaaga accatgtacc ttgatacttt gagagtcctt ctgacttttc cgcttgtttt
    38701 ttctttttca agtaaataaa acttgagaga acggtaatga gtgaaccaag aaaatagatc
    38761 atttttttta tcaccaaatc actcctttat gagaaaataa tcgcatagac atattctttc
    38821 ttctttattt cattgtaccg aataaaagct aaaatttctc taagactttc gactggtttt
    38881 tttactgttt ttctacaaat tatgatagga tacatagtag gaagaaaggt gacataattt
    38941 gaagtatcag caagtggaaa tagctttttt tattgaccgc ccgaatcggt ttatagcaca
    39001 ttgtatgaaa aaagacggag aaataattat tgcgcatgtc aaaaatacag gtagaggaaa
    39061 agaggtcctg ataccaggcg ctgaagtggc tattgtattt gctcctggac ctaaacggaa
    39121 aaccgcctat gatctgattg ctgtaaaaaa gaagggtgac tggtttaata tcgatagtca
    39181 attgcctaat cggctggcaa ttgatggtat tttagacggc acgatccttt taccaggatt
    39241 aactggtcca tttacggtat acaagcgtga agtaacttat cttcactcca aatttgatat
    39301 ttatttagag acggtggtcg gacaaaaagc atttgttgaa gtcaaaggta tgacacttga
    39361 aaataaagcg attggtgctt ttcctgatgc gcccacactc agaggattga aacatatagg
    39421 agaattaacg tatgccgcac aagatggtta tgccgcttat gtcttgttta ttgctcagtt
    39481 cgaacatctt catctggcaa cgatccatga agaaatgcaa ccagcactag ctgatatggt
    39541 tcgacatgcg cagcaaagcg gagttcaaat cctcgcctat aattgtcaag tatcggcaga
    39601 tgaggtaact gtcaagcagg ccattccatt tgatgtagat atcccttttg aagatcctat
    39661 acaatagaaa gaaggtaaaa taatgatcaa cttaggtatt atcggaacaa attggattac
    39721 tcatcaattt gtccaagcag ctttaagcac aaaaaaatat gaattgacag cggtatattc
    39781 tagacatttg gaaaaagcac aggaatttgg ttctcattat gaaggggata tcgaattcgc
    39841 aacagatttg aagacttttt tcaatattac tcatatggac accatttata ttgcttctcc
    39901 gaatagtctt cactttagtc aagctaagca agcaattttg gcaggaaaaa atgtgatagt
    39961 tgaaaagcca gctttttcga cgccaaaaga aatggatgaa atcatccatt tggcaaatga
    40021 acacaatgtg tatttttttg aagcagcaag aaatatccat gaatcaggtt ttaaaaagat
    40081 tgccgattac ttaccattaa aagacgagat aatcggtgca aatttcagct atatgaaata
    40141 ttcttcgcgc tatgaccaag tattagatgg agaagaaccg aatatcttct cgactcattt
    40201 ctcaggtggt gcattgatgg atttaggtgt ttaccttgta tatgcagctg ttgcttggtt
    40261 tggaatgcca aaagaagtcc attatttccc aagaaagatt tctactggtg tagatggtct
    40321 tggatggggg atcttacgtt atgagaattt tgatgtatcg atccagccag gtaaaatcgg
    40381 tgattcttat ttaccgtcag agatttattt tgactcagga acgctagtga tgaatggagt
    40441 caatgcaatt gaacaagcac aatttcatga tcgcgcgcat aaagaaatca ttgatttggc
    40501 ggtaacagct gcagaaaatc caatggcaga tgaagcagag gattttgcca atgtcatggc
    40561 tcatccgaaa gatccagctt ggggattgtt gtatgaagag tggatcgagc tagctagaaa
    40621 tgtgaaccaa gtgatttatg atttaagaaa aaatggcggc atcaaattcg atgcagataa
    40681 tgaggaagat ttttaatgaa cgaatttgta caagtatttc ccgaaaattg gcagaaaaaa
    40741 tatgaggata aagggtttat ctcaccttct cttattcaac gagcagtttt tcagccacta
    40801 agtaataaac aaagtatagt agcggtttct cctacaggtt caggtaaaac actggcttat
    40861 ctttggcctc tactactaaa cgtggaacca ggtgaagcaa gtgcgttggt catttttgct
    40921 tcttctcaag aactagctat tcaagtagca gatgttgcac gtgaatgggg aaaagacaaa
    40981 gagttgaaag tccaatcttt ggtgggcgga gccaacgtca aacgtcagat tgagggtttg
    41041 aaggaaaaac cagaaatctt gattggtaca ccaggaagaa tattggaatt gatgaaagca
    41101 aaaaaaataa aagcacatca agtgaaaaca atggtatttg atgaagcaga ccagctattt
    41161 gacgctggga acagccaaat cattgatcaa attcttcatc aagctccaac agagtatcag
    41221 ctagcttttt tttcagcgac agcagatcgt tcccttgaaa gcattgaaaa aatcactggg
    41281 aaaacgtttg agacaattga cgtgacagca gaggacgatt ctcgaaaagg actgagacat
    41341 tactttttac gtgtaccgat acgaaagaaa gatgactact tgagacgtct cacgcatata
    41401 gaagattttc atggtctgat tttctttaat caactgactg aattaggcgt aatggaagaa
    41461 aaattgctgt atagaggtgt accggtagga agtctggcct ctgatcagaa caagctactc
    41521 cgaaaagcag cgctagaaca attcaaaaag aaaatggtca gagcactttt gaccacagat
    41581 gtggcggcta gaggtttaga tattacaggt cttccttatg tagtgaatgc agaagttccg
    41641 ttaagtgaag aagcttattt gcatcgttcg ggaagaactg gtcgaatggg taatgaagga
    41701 atagtaatta cactcgtaca agataataat ctaagagatt taaaacggct tttacgtcca
    41761 tcaaatataa cacttgaaga gattttttta tacggaggta agctgcaaac tgagcagcca
    41821 accaaagaag atgcagctcc tgcagtaaat aaaaagtatg cgaaaacttt ttctggaaag
    41881 gagcagaacc cagaatataa caaagaaaaa ataagaaaaa aagttaaaaa taaatcaaaa
    41941 aaagagagaa ataaaggagc aaggcgcaaa taagcgcctt tttatgctat gattatgaaa
    42001 acgggaacaa aattggttgc cagaggaggg gttatgtgaa agaacgtcaa gaagagatct
    42061 tgcagctatt gacagaaaac caacaaggat tgacagcaag tgatgtagct gagcgcttaa
    42121 ctatcgatcg aagcaatgca agccgatatt taagtgaact atacaaggcc catcatattg
    42181 taaaaaccgc tggccgacca gtcgtttact ctttgccaac ggaaaagtca aaatctgatg
    42241 aagtgcatgt cgactcttct acgcaagtga cttttgaaac actcgttgga gaaaatgatt
    42301 ctttaaaagt cagcatacag caggcaaaag ctgcgattct ttatccaccg cgggggttgc
    42361 atacgattat ttttggagaa actggtacag ggaagtcgat gtttgctgaa tgtatgtatc
    42421 attttgcaat cgattcagag atgctttctg cagatgcgcc atttgtttct ttcaattgtg
    42481 cggattatgc tcaaaatccg caactgctct ttggacacat ctttgggatc aaaaaagggg
    42541 cttacacagg agcagctcaa gatagtcccg gtctaatagc caaagcagac ggcggtatat
    42601 tgtttttaga tgagatccac cgtttaccgc cggaagggca ggaaatgctg tttactttca
    42661 ttgataaagg tatttaccgt ccgcttggag aaagtgcaca agtacatgaa gcatctgttc
    42721 agatcattgg ggcgacgact gaatcttcag aaagtttctt atctacgttc aatcgacgga
    42781 ttcctatggc aatcaccttg ccgaatcttg cttcacgttc actggatgag cgttatgaaa
    42841 tcatctctct ttttatcaaa caagaagcga atcgtttgaa tcaacgtatc gatatcgaaa
    42901 aagaagcgat tcttgccttc atgctttatg atgcggaagg aaatatcggt caagtgaaac
    42961 gagacttgaa actagtctgt gcaaaatctt ttttacatta ccgtactcat catgagaaaa
    43021 agctaatcat tcgaaaagaa gaactttccc ttcaagtaca aaaaggatta ctgaaaatca
    43081 aggaaatacc ggaacgtctc gaccgattca tggacagcaa aagtcagtat ttgacatttg
    43141 aaccaggatt tgctgatgtt gtctggtcac aagatccaga acgaaacatg caggtctata
    43201 acgatattga ggaaaaagtc acttctcttt ccgaaaccgg tgtggagaac attgatctag
    43261 agaccctcat ttcaaaagat gtggatgctt atttccagac atatgtcaaa gaattgacta
    43321 aaagttcgat tcccaaagat cttttgccag atgaaatctg gcggttaacc aatcaattgt
    43381 atgatgtggc tgaaaaacgg ttagaccgta tttataatga aaaagctcgt tttgcttttg
    43441 ctttgcatct gcaaagtaca ttggatcgtg tgagagaagg tcatatgatc gtccacccag
    43501 atttgaataa cgtacggaaa aattttaaga gggaattcca ggtagcaatc gatctttcaa
    43561 cgatcattga agaagaagaa caagttgaga ttccttttga tgaaatcggc tttattagta
    43621 tgtttctttc tatcaatcta ggagaaagtg agcctgttaa agaaaacaaa gtagatgttc
    43681 tcatattgat gcacggcaga cagactgctt caagcatgtt ggagatggca caagaactat
    43741 taggaacaaa agtagggaaa gcttttaata tgccactggt tatggaagtt caaacaatgt
    43801 atgaagaagt gttacaatat gtcaaaaact atcaagagca tttgacgaat ggcctactct
    43861 tgttaacaga tatgggatca ttaaacacct tcagtgattt gatttatgaa gagactggca
    43921 ttcggacgaa agctatcacg atgacaagta ccatgattgt gatagaagcg atacggatgg
    43981 caagtgttgg aagaagctta gaagatattt atcaaaatat ccagctttcc tttgaatcga
    44041 tcgtacgtga tcagttcaaa gcggaaaagt ctgaaacttt acagcgtaaa aaagccgtaa
    44101 tcgtgacatg ctttactggt gaaggagtag ctgccaaatt atatcagcgt attatgccag
    44161 tgattaatca aaataaggta gaactgatcc agatgcagtt tttagaacgt gaatcattca
    44221 aaaaacacat cgatgcctta ttggaagaat atgagatcaa agcaattgcg ggaacagttg
    44281 atgttgatta tcaaaatatc ccattttttt cagcctatga cgtttttgat gatgaaaaac
    44341 taaatatttt aaagcgtata gctgatgatg agatcccgac agctaaaatt gtcgattcac
    44401 ttgctggatc attaagcgat gtcttttcta ttgatgaact gatccgttca ttgcaaaaga
    44461 cagttcagca gatccagacc gatttgtcga ttatcgtgga acctggagta gatactggaa
    44521 tcgttatcca tttagccttc atgttagacg gattgatcaa acaagaaagc catcggcaat
    44581 ttgagaaact gtctattttc tcaaaaattt atcgtttgga aatggatatc gttcgggcaa
    44641 acttgatggc actagaacga aaatatcagg taaaaattcc agaagatgat atcgcatttc
    44701 ttacacaaat gtttcttgaa aataaaataa aaaaatgaac acactaatca tactgtttac
    44761 acactaaaca tctgtgtgcg cactataaaa aagagctaaa aagcatgaaa acgctatttc
    44821 agctcttttt tttattggca tgcttcttgc gttatataaa agtgtaaaaa aataaaagga
    44881 gtgttaaaaa tggcaaaaaa aacaatcatg ttagtttgtt cagcagggat gagtacgagt
    44941 ttattggtca caaaaatgca aaaagcagca gaagaaaaag ggatggaagc cgatattttt
    45001 gcggtctctg cctcagatgc agataataat ttagaatcta agaatgtcga tgtattatta
    45061 ttaggtcctc aagtacgttt catgaaagct caatttgaac aaaaacttgc gcctaaaggt
    45121 attccattag atgtcatcaa tatgcaagac tatggcatga tgaacggaga aaaagttctt
    45181 gcacaagcag aaaatctaat gaaataaatc aagtatgcag ttgggggtat aacaacgtgg
    45241 aagatcaaca aaatctagaa gccatcatgg ggttaatcat gtttggcggt aatgcaaaga
    45301 gcgatgcaat ggaagcgatt gcggctgcta aaatcggtga ttttgagtta gcggatagga
    45361 aaatcgctga tgcagaagaa tcacttatac aagcgcatca tgcacaaaca gacatgttga
    45421 cacaagaagc acaaggaaat catatgcaag tgacattatt gactgttcac agtcaagatc
    45481 acttaatgac atctattgcg tttactgatt tagcaaaaga aatcatcgaa ctttatcgtc
    45541 gcgtatacag cgaataaata caacttcttt tttgaacgga agaagaaaaa tttttgaacg
    45601 gattttttat tttgttataa ggagaactct ctttatataa aaaacttata ggaggaagta
    45661 aaaaatgaac ggactatcaa gttggatgga gaaatacatc ctgccggttg ccgcaaaaat
    45721 cggagcgcaa aaacatttag ttgctttgcg tgatgctttt atcggcacac tgcccgcaac
    45781 aatggcggga tcagtcgctg taatgatcaa tgcaattatt cgtgatttac cgcaacagtt
    45841 taacagtacc tatgctgaca gtttagcagc agacaaagga atctttgccg tattgaatgt
    45901 catcatcgga atcaatggtt ttgtctggaa cggtacacta gcaatcgccg gattgatctt
    45961 tgccttttct tgggggtata atcttgcccg agcttatgga gtaaatgatc tagctggggg
    46021 gatcgttggt ttagctacct tgattcaagg tatttccttt ggtccagcat tatcagcagc
    46081 attagatgtg aatgttcctt caaatgtcgc agatgcaatc aatagttctg agattggtgc
    46141 aacgattgca gatggcacac tgtcagttgg attatggggt tggctaaaac tagatcattt
    46201 gaatggaaac gcttatttta cagttatgat catgggagct atttcagtca ttattttttg
    46261 taaattgatg cttgccaaca ttacgatcaa aatgcctgat tcagtaccac cagcagtatc
    46321 taaagcattt gcagccattt taccagcgac gatcgcttta tacgtcattg ctattatcaa
    46381 ttatatcgta ggtcgtgtag ctgatgggca actgatcatt gatttggtgc aaaaatatat
    46441 tgcagaacca ttcctaggat tatctcaagg gttgggagca gtattgatcg tcacagtatt
    46501 tgttcaagta ttctggttct ttggtattca tggaccaaat gtattggcac cagtgctaga
    46561 gggaatctgg ggacaagcac aactaatcaa tattgatatt ttccaaaaag gttataacgg
    46621 aaaaactggt acaccagccg tgttagatgc aatcgaagat ggaaaagctt atatgtgggt
    46681 ccgcggatcg ttcgatgctt ttgcatggtt tggcggatca ggcggaacga tcgtcttact
    46741 gatagctatc ttgattttct ctaaacgtgc agattatcta acagtagcta aattatccat
    46801 tgggcctggt atcttcaata tcaacgaacc agtgatgttt ggtctgccaa tcgtattgaa
    46861 tgcaatcatg tttatcccat tcttgattgc tccagtagta gcaacagcaa ttggatggac
    46921 agctacttat cttggcctag tcgctccggt atcgcagcaa gtcacatggg tagtaccgcc
    46981 gttcttattg tcattcttag caacaggagc ggattggcgg gcaccgatcg taacattagt
    47041 atgtatggtc gtgactttct tgatttgggc accatttgtc atctctgcaa acaaaatgga
    47101 ccctgccgca ggcgaacaac aataagatga acgtgaggag cgggacgctt taagtttcgt
    47161 tcctcataat gaattttcta ggaggaactg aaatgattac aattgccagt gcaatgcaac
    47221 aagatgaaat caaagaattg ttagaaggat acaatgaaga aggaatgact tttacattta
    47281 aagaaaaaca aggaatcaag ctgttctttg aaacaagtgc agaagatccg gaagttgcgg
    47341 ctaaaaaagc aaaggaacta atcaaagcac agccttgggg aacagtcctt tattttcaat
    47401 caacagctgt ttagaaaatg gtttaaaaag ataggatggt tgagatgata aaaaacggtt
    47461 tatttttatg tagtattggt tctttgatcg tgttgtatag tttgaatcca cattcgcaaa
    47521 ggtatgattt gtatagtatg gtcacaggtc tttttctaac aggattagga atctatttct
    47581 tcatgaaggg gaaaaaaaga gaagaagcaa aggagaaaaa taactaatgg aaatcacgaa
    47641 aaaattaaat attccagcat cgttttttta cgataaagtt atggactctg tccttttcga
    47701 tgtacgtaaa gcgaccggca aatcagtaac tagaaaacag ctgaaaaatt tggagtacgt
    47761 caaacaattc tcgcaaaata accgggctcg cataaaaatc gaagaagtca ttgaaaacca
    47821 gtcttataag tttcgtacat cgacaacgaa gaatgaattc gtcgttcatt atcaaatcaa
    47881 accattggac gattcgacct gtgaggttca ttacacagaa aaaatggagt cttatggcat
    47941 gcttcaaaag ttgaatgata tgcttttagg aacaatcttg atgtatttta aacggagacg
    48001 tttcaaaaaa atgctagata tgatcgaagc aacgtattaa atcaatgaaa catggtacta
    48061 tttagttatt gaaaaatgaa gtaagctaga aggaattttt cataggaaga tagatctttc
    48121 ctgcgaatcc tcactagctt ttttgtagga aggggcagca aaatgacaaa caaagtaaga
    48181 gagctaggtg tatcttcgat tgaggaaatg tttgatttag ccacttatgc atttaatggg
    48241 gcagatacac ctgaaaggcg agaacgtttt cgtacacttg cggagaattc ttggaattac
    48301 ggattttttg atgaggagga tcggctaaca agtcaagtca tggcgacccc ttttccagtg
    48361 aatttttacg gacaagagta cctaatggcc ggcattggct atgttgcttc ttatccagaa
    48421 gcaaggggac aaggcgggat caatcagatc atggaaaaga tccttgaaga ttgccgtgat
    48481 cgtaaagtaa gcttatctta tctggcgcca ttctcctatc ccttctatcg gcgctatggc
    48541 tatgaacaat catttgataa aatcagttat caactaaaca gtcgtgatgt gccgtttatc
    48601 aaaaaaaaat ctggaaaaat aaagcgtatg gattttgaag atgcaaaagc tgcaatcaga
    48661 agaatttatg aacagatgcc ggaaaaccaa cgagctggtc tgaagcgcga agactggtgg
    48721 tatacgtata aattcaagca aaaaggcaat taccagtttg ctttgcattt tgatgataat
    48781 gaggaagcaa cgggatatat cgtttatcag cttgcaacgt cttcctttat tattgcggag
    48841 tggggatttt tggatcatga tgctttttgc agccttgttc gcttcgtaag ttctcacaat
    48901 ggtgcttttg aaacatttga atattcatgc ggctacggcg gtaatgatca aaattatcta
    48961 ttagaaaatc catttgcttc tgtaatgatc acaccttata tgatggcgag gatcgtagat
    49021 atttctttgt ttttggaaaa ttatccattt aaaaagagac aagctgcatt tgccctacaa
    49081 atagaagaag atcattatgc gaaatggaat gaaggaatcg tggaagtaac aatcgaaaat
    49141 ggaaaaaatc atctctcaaa agtagaacaa acaaaactcc caatcgttcg aggaagtatc
    49201 caacatatca cgcagctatt attagggtat cgtaaagtgg aagagttggt attccaagaa
    49261 agaatacagg tgacaaaaga atatgaagaa acgttaagct caatcttgcc acagcaacag
    49321 ccaattctta atgactattt ttgataaaaa acgggtgatg acaatatttt tgtcaccacc
    49381 cttttgtttt tcatttggca tttgaaggaa aatgttaaag taaatcctct tttttcgtaa
    49441 atttgaattt tttgttttct tttgttttag ggttttcttt tttgtggtct tggattgctt
    49501 ctttcgtaat cttttcccga ttttttttag atgaatctaa gcgttctgcg tatacttttt
    49561 cttttggctt atcattcatt ggaaaggacc tccctacatt atttcattct acctctagtt
    49621 tatcatctgt taaggactag tggaaacgtt ttgcctaact tataagctga tttttttagc
    49681 agataagctt gcaagtaaaa aacaaacaag ctacaattga tttattgaaa ttgttcctgt
    49741 agctcagtag gatagagcaa tcgcctccta agcgataggt cgctggttca attccagtca
    49801 ggaacgtata aataacccct gtatcctttg cggtacaggg gtttgttttg tatttgtgcc
    49861 aaaaatgtgc caaactagtt atcattttta tataaactat cgaacacagt attgattttt
    49921 tctttatcct gatcttcaag ctctcgaaca atatgaagat aagtagacat tgtagtttct
    49981 aaactgctat gtcctaaacg tttcgatacg ctaagtatat ttactccttg ataaagtaaa
    50041 atagatgcat gtgtatgcct tagtccatgc aaagtaaatt gcttttcaat ccctaattct
    50101 tttaattttt tgcgcaaata tttgctgacg gcgtttgatg aaactaatcc attttttaag
    50161 ttgaaaaaaa caaaattatg agggtttttg atttcaaaat tctcgaataa ttctttttga
    50221 ttgattttaa acttttttaa gagatcaatc gtatgtttat caatggaaat tttcctattt
    50281 gaagtttcgt tttttgtttt tccccattca tttaatttat aattccatgt tttattaata
    50341 gtgatgattt gatcttcgaa atcgatatct tcccatgtta atcctagaag ttcagcaaat
    50401 cgcattccag tggcaccagc aactaacacg agcataggag aagagtactg cgcagaaagg
    50461 ttttcttcta cgagtttcat taaagattta aattgatcat aatctaaata tttatcttct
    50521 tttggcttca atgaatcttt tcctttgatt actgcttttc tagttggatc aaacggaatt
    50581 aatccttctt ctacggcatc tttcaacgac gctctaatat ggttattaaa cttaataacg
    50641 gtagatttta catggtcttt agcatactta tttaaaaagc gttgatatcc cactctatct
    50701 aaatcagaaa tcaataccgc tggcatatat ttctttatgt tcattaacgt atcttcgtat
    50761 tttctataag tgataggaga tactgttcct tctttataaa gttgcatcca atcctcaaag
    50821 taatctgata ataatagatt tttgcgatcc cctttaaggc ctttgttcaa gttatattct
    50881 aattcgttgg cggcatcttt tgcttctcct tttgtcttaa atcctgattt tcttatcttc
    50941 gtgtattttc cgtcatcttt tttgtatgaa atttcgtatt gccaactatt tccacgtttg
    51001 actaatcttg ccataattga atcagctctc tttctctgat acaataggaa ctataaagaa
    51061 gcccattgtt tgggttttat tattttttgc acatcctttt tgcttgccgg cgcaaggatg
    51121 tgctatttgt cgttttaatg gtataattgg atgtgtatta gatcatccaa tcagaggtgt
    51181 ttattatgat taaagtgatt tttaaaagaa taactgaact tccagagttt agattatttg
    51241 ttgattattg gtatgtctgg ttttttattg ttgcattgct gataggtcta gatgtcatat
    51301 tagaaagaaa acttaagtaa gagttgagtt aataaattga attcctaaat gagatatttt
    51361 gtagcccata ttttgtttgc cgacatcacc taatactatg gagctaatcg ttttttctaa
    51421 cagaccaaaa tcagataatc tatttaagct accatctatt agctcttgtg aatagccaga
    51481 catgctgata tttccagtga aattttcgtt tccacgactc attaagcatg ctaacaattg
    51541 aatctcaatc gatgttaatt gattcagtac aagaagataa tactcctcgt tatcccatga
    51601 agcgctgtta attcgaagta aacaattctt ataaagattt ttatacatat ctattttatt
    51661 ttgtgccttt gatttttcta cctcgtcatt tatcccttcg aatattccaa tgagttggtc
    51721 cttgttctct aagttgaaaa catcttctgg aatttgatcg cgaatgtttt ctaatgattc
    51781 gtttaaattt ttgtagaatt tctcgatgcg tttgaaacgc ttctcgtttt tagacccaaa
    51841 ataaagtgac tgcacaacgc caccaatata aggaattgct ccaataccgc cttcaactat
    51901 taattcaaga tattcttttt tggttagttt ttcattgcta ttatccatag ttacctccta
    51961 ttttgtcatt tcctttacaa gcattgtgac tcctgatggc actcttttgt ataggtttta
    52021 ttattgtgca caatctaact cttagtgggg cgtggttcat ttatttttta cttactttac
    52081 tattaatcat atcccattga gcataggatt tttctatgtt cgatcctctt gaactaattt
    52141 ctcctgtaaa agttacttta tcgccctttg taaaggtatc agcattgttt aagtccttcg
    52201 caaaaattac atatgaaact ttaggtgttg cagaaatatc attccaactt ttatcatcgt
    52261 actctttgtc tgctataatg gctattctag tagtcattga ttcgataaca gttccttcaa
    52321 aagtatatgt tttaccacta atatatttag taaaagctgt attttgctca gtagctggag
    52381 taatagaata ataactatct aagaaagcac cccagtcatg attatttgtc tctaaaaatt
    52441 cttcaaattg agttaggctt ttattgctgc tgttagatgt agtagtttga gtatcagatg
    52501 atgagctttc ctgtttgtta ttgtcatttg cctcgtttga tgaggaaagc gagaaaccta
    52561 cggtagtaaa aataatcccg ataataagga gaatcatagg tgttttctta tttgtttttt
    52621 tgataaatct tacaattaac atgattaaac ctataataaa taaaacaata ccagtaaaac
    52681 caaatatacc gtccattaat agatcctctt tctcttttga tattgaatat tataaaccaa
    52741 gcaattcttt tttcttaaaa tcgaattctt cttgattaat aatcccttca tctaacaact
    52801 ctttatactt tttaatttca tctgcacttg aaatttgtgt ttctttggaa ttattatcta
    52861 atatagattg ttgcttctct atatattttt ttaaattttc catttcattc caatattttt
    52921 ttgtaaacat aacggtgttt tcatctttcg tggccgctaa tatacctccg cgactttcgt
    52981 ttccgcctag caaggtaaat tgtatataac cattactcat acctggtttt tttaattgta
    53041 cggctgatat atttttaaag gggatagttt tttctccttt aataccttga ttaactaaat
    53101 ttataaatcc ttttctaata attgaaatag aatcatcagt tactttaatc aaggtttttc
    53161 ctggagattt tattaaaatc tctttttcca ttttttcttc ctcatttctg tgatatgatt
    53221 ttttttgtag aagctcatat atgaggaaag agcccgtgtt gcagcacggg cttttttaat
    53281 gttttgctga aatcggtttt tttaataatt cttgatgtat tcatacatat tgccttgcgt
    53341 aagaatattt tttcttaaaa tagcattggc agacagcata acaagtgtat ctgagcttat
    53401 tctgaataga ataatactcc ataaattttt cgagattaaa ctgagattca tcagtcagtt
    53461 cgttctcaat atagatattt aataaaatta gaatagctat tttatctgct tctgtttcaa
    53521 attttgagtg aaaagttgta gaagtatcgt acaaaactga aaattcaaaa tgagaggcgc
    53581 tgaaatgtgc aagttcatga gataaatgaa aggcttctgc agtttcaccg tatagatttt
    53641 cattcaaaaa aatgattctg ggttttggat agtagaaacc aggctctttc atctccatat
    53701 aaactacttt caaattgtat tcgctcaaca tttctttcaa tttcaaatac atacaaacca
    53761 tcactccaac tattcatttt cctctaaagc tttagcaatt gcaatcgctt tacgcattgt
    53821 ctccttagat atttcttttc catcaaaaga aaaaacagta tcgtcttctg ataaatccac
    53881 atgtttaggg gtttctcttt cttctctacc tagaaggtag tctacagaga catcgaaata
    53941 gtcagcaatc cgacttaatt catctgaatt aggagtatta tttttccact ttgctagata
    54001 accgttagaa tatccaaaat taatttctaa ctgccgtata gatatctttt tcctcttcgc
    54061 caagtctttt attatttcat aggtattcat cgataaaaca acctttctaa atgtttacaa
    54121 aaaaagttta gaaaaatacg cagaaataat ttgactaatt ctgagtaatg agctatacta
    54181 tatcttgtaa acagatttaa caactaaaaa gacaacaaaa aacactattg atatgtaaat
    54241 gcagaccgcc aagaaagctt taaaatcaat gtttttatgt cttatttaat tatgctctta
    54301 gtatagagta ttactcagat actgtcaatt gaatttagaa aaaagttgtt aaatttgttt
    54361 acgaatataa aagaaaggaa tgagaaatat gaacacaccg caaattttta atttcgaaca
    54421 aaacgaagtt cggacaattt tagtaaatga tgaaccatat tttgtaggca aagacgttgc
    54481 gagtgttttg ggttattcaa acactaaaga tgctttgtcg cggcatgtag atttggaaga
    54541 taagatgggg tcgcgaatca cgacctcagg tcaatcaaga gagatgacaa tcatcaacga
    54601 atctggtttg tacagtttaa tcttaaaatc aaaacttccc tctgccaaaa aatttaaacg
    54661 ttgggtaaca agtgaagtgt tgccagcaat tagaaaacat ggaggttatc taactccaga
    54721 aaaagtagaa gaagctttgc ttaatccaga tacaatcatt caattagcaa ctcaactaaa
    54781 agaagaaaga actggaagat taatcgcaga acaaaagatt gcagagtacg aacctaaaat
    54841 ctcctattta gatagcatat tatcttctac agattcagta acaattagtc agattgcagc
    54901 agattatggg atgtctccac aacagatgaa taaattactt cataaactag gtattcagaa
    54961 aaaagtcggt aaccaatggt tattgtgcaa aaaacacatg aaccaaggat acacaaaatc
    55021 tcatacaact gagatcccga aagccgatgg tggcactaaa attgtaatga ataccaaatg
    55081 gacacagaaa gggcgtctat ttatctacga attactaaaa aaagaaggat attaccctca
    55141 aatggattta gaggaaattg gttagaaagg agttttagta tgactgacat tgcagaaatc
    55201 actcaacgag atagagaaaa aatcaaagaa tatgtcgaaa gttcaaagtt cttaacttac
    55261 accatgcttg ctgaaagatt tggaattagc aaaagctact tatctttaat tttaaacggt
    55321 aaaaagactt ctgcagaagc aaacagaatt atagattcga ttatcactat gtacgaattg
    55381 taaagggagg aacagctaat gcaatatcta gaagcaaaaa tcccaattcc agaaggctat
    55441 gtaattatct cccaagtgga ttatgaggag ttaaaaaaag ctgatgatac tggtagatgg
    55501 atgacgttgc cagaagtgct agaacggatt aacagaaaat atgattggtt tacttctaga
    55561 gttttaaaga acccaagata tagaaatatt atcgatatcg agaaaaacaa aaatggattt
    55621 gtctattatc cagttgaagg aagagacaca tatctatttt taagaagtaa aacacttgaa
    55681 tttttagaaa caaatttttc ggaaatctta aggaggcaag cggatgggaa aatttaatag
    55741 agcgctagtg ttcagcgcac cgctaatcat ctacgcttta ggactttggg gaagcaggca
    55801 agcgttgata ggaacgattg tttacatggt ttggattttt atggggcttg atgaagctga
    55861 agctgagtac agagcgaaaa agccaaccga gggggctgac taaaaatgaa tagaaaagag
    55921 aaactagaat gtatattact attactcagt ttaattcttt cactaatttc tctattggga
    55981 agtttgtatt tttgatatca acaattttgt ggatttgggt tctatttaga gtaaatatca
    56041 tttttgtttg gtgattaagg agtttattaa aattaatttt ccctacttca aatgcaaggt
    56101 aacaagatat agcactttta ggaggaatat ttagcggtac agcttcagag tatattctat
    56161 tatcatcgac tgatcctcct ttagcaatta atttcttaaa tggagtagct gaccatctgc
    56221 ggtttaagtt agcggatgaa agttctaaat taactaacat agctggttct gttgaatagt
    56281 tagaaatgat tacttttgta taaatcatat ctgcagcgaa gtaagaagca ttaagttcaa
    56341 cttctagttg agggcgcatt ccttttattt ttatccctgt taaaaacgta ctcaatataa
    56401 aacttattat tgacatccat tgaaaaattg ttaagtgaaa aaaattcaaa ataaacacca
    56461 ccagttttta actaaattat accaaaaagg agagaagaaa taatgcaaga attagtaatt
    56521 ttgaaaaata aagaagctgt gactacgagc ttacaagtgg cagaaagctt cgagaaaaaa
    56581 catcaacatg ttttaagaga tattgatgca ctaaaaaaag atgtgtccaa ttttggacag
    56641 atgtttgtag aaggtaatga accagattca tatggcagaa atcgacgagt tttcttcatt
    56701 agtagagatg gatttttctt gctggctatg gggtttacag gaaagaaagc tatctacttc
    56761 aaacaaaaat acattgaagc attcaacgaa atggaagatg ttattcgcaa gaatactgtt
    56821 cctcaaacaa ttgaagatat gatgatctat caattagaag aaatgaaaga tgttaaaaaa
    56881 gatgtttcca tgcttaaaga tactatgcga attagcggac aacaagagtt tgaaattaag
    56941 caaaaaggaa atatgaaagt tatgggagtt ctagggggaa aagaaagccg agcttatgaa
    57001 gaaatcagca aaaaagtatt ctcaaaattt tggtctgaat ttaaacgtac cttttcaatc
    57061 ccaagatatg gcgagttacc tcgtaagaga ttcgatgatg ctgtttcatt tattgaaatg
    57121 tggttgccag aaactgcgat ccgtatggaa attgatcaac tgaacagaca acagagactt
    57181 tttggtgatg acaatgaata gagctgaagc gctaagaata gggacggcaa ttgctaattg
    57241 ctggtggaaa tactataaac caatcatcct aagccaacaa catattgaca agcaaaaagc
    57301 atggcaacaa ataaaaaagt gactccgccg gcaagcaaag agtcacaaag aaaacacatc
    57361 ataaggagat tttagcatat ggaaaaagaa ctttccactc tagatcaata tttgattgat
    57421 cctgattggg gcaagccgaa aattgaggaa acaagtggtc gaaaaatcag acgaaatctt
    57481 ttgacgaatg aagaactagc ttgggatcaa gatgatttag gcaaccatgt aactatttgg
    57541 gatcatgttt atcttatcca tctatcgaag cattcgaata aacctgaata tatttacgtc
    57601 atcgaagatg gcttgattga tgcgctagaa gagtacgaca gagataactt gattgatatc
    57661 tcttattacg gaccaggtaa gaaatacatt gctgaaatgg aggcagaatt tgatgagtga
    57721 aatcaaaggg acaacgaact ttgaaaaact ttttagtcgt aagttaaata aaattctcaa
    57781 gaaaaaagga aattttgatt atttatcttg ggctcacgcg tgggagatta tgaaaaagaa
    57841 tgatccacag gcaacggtaa ctattaatga gtacaaacac tacagggttg tttctggaac
    57901 tcatcaagac tttcttgttg aggaatataa accttttctt atggatgaaa ctgggactta
    57961 tgtatctgtc tcagtaacgg ttaaaggaca cacggaaacc gaattatttc ctgttttaga
    58021 ttatcgaaac caaccagttg ttaaaccaaa tgcaatgcaa atcaataact cattgaagcg
    58081 atgctttgtg aaagcattgg ctctacacgg actgggatta tatgtatttc aaggggaaga
    58141 tattccaaca ccacctagaa tcgatacaaa gaaattaaac atgctagaga cgattctaga
    58201 agctttcaat gagcagatgg gtaaagatat gaccaaaacc ttaatcgaat atgttaatga
    58261 gcagacagat aaattagggc tcttagctga taacgttgaa actattgaac agttaagcta
    58321 tgagcaatgt gccttgatgg agcgagcaat agcagctaag agaaaagaat tagataagaa
    58381 gtgatatgag tgtttaaacc attaatcgat tcatattcag cggttctgaa aaagttcaaa
    58441 ggaaaagaca taagcgcaac catcaatgag gaagtgaaca ttgatcgact aaagacgatg
    58501 tatgacggct acgatggtga tcgagtcatt gaaattcgtt ttattgatcc tagacgtttc
    58561 accgtacagc aacgaaactt catctatgcg ctgataggcg atatttttat cgatacaggc
    58621 atgccaacgg acttctggaa ggaattcttc tactttcgtt ttgaaggtgt cacagggcgc
    58681 aaaataagcc tgaaagacga atcgaataca actgtgagtg atgccaatgt cttagcaaat
    58741 atcatcttag atttcatctt tgaacatcat attcctttca aagaaggcta tgagatttta
    58801 cctgcgaatc aagaatatta cttctacaaa tgcatcacaa aaagagtctg ctgcatctgt
    58861 ggcaaaacag gagctgacat cgatcacttt gacaaagcgc taggaagacg aaagcgcaaa
    58921 gaagttgatc atgcagagta cacatttgca gcactctgca gaatccatca cacagagaag
    58981 cacaaaatag gtgtgattaa tttcaaaaat aaatatcaaa tcaaagggat caaattaaac
    59041 cagaaaacaa tcaaaaagtt aaatattgga gggtaaaaat gacagaacat cgaagttatt
    59101 atgcgattat accagccaac gtaaggtacg acaaaagact taaaccaaat actaagttgt
    59161 tatacggaga gataacggcc ttgtgtaatg aaagaggctt ttgttgggca ggcaatgagt
    59221 actttgcaga tttatatggt gtgaataaag agaccatatc gcgatgggta agtgatttga
    59281 ttaagtttgg atacttgaat cgggaaatca tttacaaaga gggtaccaat caaataatca
    59341 ataggtacct acgaattaat caatacccta ttgacgaaaa acgcaatacc cctattgacg
    59401 aaaaagtcaa agataataat acatctatta ataatacatt taataataca aaagaatata
    59461 taagagagtt accgccttcg aaaaaatcga aggctaagcc cgtccgtcat aaatacggag
    59521 agtataaaaa tgttcttttg tcagatgagc agatggagaa actcaaaaca gaattcccta
    59581 atgattacca agagcgaatc gaacgactgt cagagtattg tgaatcatct ggtaagactt
    59641 ataaaaacta tttggcaact attcgaagtt gggcaaggaa agaaaaaagt gaacctaaga
    59701 acgcaagcag tggatacaag cgcacaggaa gacgagagaa gcttcctgaa tgggcaatcg
    59761 accaagaagc ctatctcaag aaaaaagcgc tagaacgagc taatagacaa tcaaaagcac
    59821 cattctaaga ggtggaaaaa tgaagatcga ttatctagaa ctaattaatg aaatagcgaa
    59881 ttataaaaag ggcgaggaat tagacgtcct gagagacgta tatgatcaac tcgaagaagc
    59941 tggaattgaa ggaattaaga atgatcgttc gagttggagt aaactcagat actatttcgc
    60001 actctatatc gatggaacgc aattaagaaa tttagcatat accaaattac tattcattga
    60061 ttgtgttaaa ggcttgcaaa aacatcttaa tgaacttgag caggtgtaaa caagatggac
    60121 ctaaagacat ttacagcaca gatcgaatta atgcatcaag aagctttaag acaaagtgtg
    60181 tcgtacgaag acaagtggct caacacattt catggcggac gtgagagcgc acttgatcaa
    60241 gtactcaaat tactgaaagg agaatgtcgg gatggataag aaagcggcaa tgcagcgaat
    60301 tatcgaattg acttattcag aagattggca ggaagataaa aaaatagttg cagaagtcca
    60361 aaagctcggt aaatcaatgt ggactgaaaa gcccaaacgg agaacgccga gaaagattgc
    60421 aatctggcat ggtgatcgaa ttctagtaac gggtaccgct aaacagttat ctgaaattac
    60481 tggtctgagc aaaaatatta tttgggatag agctaggagc ttatggattg attcaaaagg
    60541 aagacagttt aggtatgtgg aggagaaata atggacgaac taatcgcgaa agtagagcag
    60601 tgggcaaaag ataagggatt ggatcaagcg gatttcagca agcaaatgtt aaaaacggtc
    60661 gaagaaatcg gggaagttgc cgcttctcta gctagaaaag atgaacatgg tttaagagac
    60721 ggaattggcg atgtagtagt aaccttgatc atcttggcaa tgcaaaatga tatggattta
    60781 tacgagtgct tggattttgc ttatgacgaa atcaaaggtc ggactggaaa gatggtcaat
    60841 ggtgttttcg ttaagtccag tgacctgaaa taattccatt aatggaagaa ataactgaat
    60901 aggaggatag acatgattga tgcaaaacaa gtgcaaaaac agaaagatgg aatgttgatg
    60961 tttgaagcat atgttctacc atttcttaat caatttgaag ttttggaatg ctctgccagc
    61021 ggagaagaat tggagtatgt tgtaattcgt gaaaccaaag aaaacgttca gaagctaaac
    61081 gaatttcttt gcactattaa ctgttgggat atgatagctc caggattttt atgtccagct
    61141 atgggagaat ttttggaata ttgtcgtctt gaagatgcag gcactttgga tttagcttat
    61201 ctggtataca actacctaaa catcaacaca gatcatttgt ggtttggcac tgccgaaaga
    61261 aaatgggtag tccgctaacc accaatatca atgaaaaaag gcggtttcct gaaagtggaa
    61321 ttgccgccat tgctgtaagg ttctttacgc gactcaaggt cacagaaata taaatgcaaa
    61381 tctcttttga agtgatccaa agctctcgga aaatggaagt tttttgtttc aaagaataaa
    61441 tgagatttgc gtcaagaacc tgagagttat ttccaaaaaa gcttccaaag aaccgaatga
    61501 gctaaggaca tcatgtgtaa acacataata tagctctcct ttcacggcta atcataacat
    61561 gaaaaatatt cgatgtcagt aggaaaaaag gtgaattatg aaaagaatat taaattatcc
    61621 cggcagtaac agattctgca attaaactcc gaagctgatg tatcagtatc aactgtagaa
    61681 tattatgagc gtggagaaag tcaagcagtc acaaatgtaa aaatcgataa cgtcaacggt
    61741 cttatctgtt tagatgaagg cgatagtatg aatgaaattg ctgcagacca tgaattatca
    61801 gaaactgctt taattgatca aatcgaatta gctagacaat actttgttcc atatgcagat
    61861 acctggtcca agcataagaa tgacatcatt tttccagagg aacaatgaga gcaactgatc
    61921 cagtaattat ccttgaggaa gccaaattta tttggactca cgaagagata gagcaagcac
    61981 gcttgctctt ttctcaagga gttaagccga gcaaagtagc tgaaataatg ggtcaaaaga
    62041 ttcttgatgt cggattgctt ttgctccatc tagcagaaaa aaatttgatt tgaggtgaaa
    62101 atgatgattc aacttgcagg catccaaaca gggaaaattt atttttctgg agaaagcaaa
    62161 agcgaagcca accaatggct attgaaaact tacacgaaca ataagaagct aaggaaaaaa
    62221 tatcctgatt tgttgctaaa ggatgatcag attatgccgg aaccgatgat tttgacccgt
    62281 aaggagggaa gttgaaacta aaaaaccagc cgtgtgggct ggagttgcta tttcttttta
    62341 ataatgagtt tttctaaatt taattcattc tcacgagttt gcgtccaata atcagaataa
    62401 tattttaatt gtttaagagt aggaatgtcg tcttcggtaa aattagaaaa taattctgga
    62461 gaatta
//
LOCUS       DAFEOM010000010        59257 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.010, whole genome shotgun sequence.
ACCESSION   DAFEOM010000010
VERSION     DAFEOM010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.010"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(300..1610)
                     /locus_tag="LP524_000762"
     CDS             complement(300..1610)
                     /locus_tag="LP524_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBL2233835.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1622..3040)
                     /locus_tag="LP524_000763"
     CDS             complement(1622..3040)
                     /locus_tag="LP524_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL2233836.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3280..4848)
                     /locus_tag="LP524_000764"
     CDS             complement(3280..4848)
                     /locus_tag="LP524_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBL2233837.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4852..5262)
                     /locus_tag="LP524_000765"
     CDS             complement(4852..5262)
                     /locus_tag="LP524_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HBL2233838.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5259..6182)
                     /locus_tag="LP524_000766"
     CDS             complement(5259..6182)
                     /locus_tag="LP524_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HBL2233839.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6179..7057)
                     /locus_tag="LP524_000767"
     CDS             complement(6179..7057)
                     /locus_tag="LP524_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HBL2233840.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7044..8381)
                     /locus_tag="LP524_000768"
     CDS             complement(7044..8381)
                     /locus_tag="LP524_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBL2233841.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8384..9487)
                     /locus_tag="LP524_000769"
     CDS             complement(8384..9487)
                     /locus_tag="LP524_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBL2233842.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9561..11069)
                     /locus_tag="LP524_000770"
     CDS             complement(9561..11069)
                     /locus_tag="LP524_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBL2233843.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11035..11727)
                     /locus_tag="LP524_000771"
     CDS             complement(11035..11727)
                     /locus_tag="LP524_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBL2233844.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11749..12723)
                     /locus_tag="LP524_000772"
     CDS             complement(11749..12723)
                     /locus_tag="LP524_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL2233845.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13023..13895
                     /locus_tag="LP524_000773"
     CDS             13023..13895
                     /locus_tag="LP524_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL2233846.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14353..16551)
                     /locus_tag="LP524_000774"
     CDS             complement(14353..16551)
                     /locus_tag="LP524_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2233847.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16783..17775
                     /locus_tag="LP524_000775"
     CDS             16783..17775
                     /locus_tag="LP524_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL2233848.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17835..19256
                     /locus_tag="LP524_000776"
     CDS             17835..19256
                     /locus_tag="LP524_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBL2233849.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19249..20748
                     /locus_tag="LP524_000777"
     CDS             19249..20748
                     /locus_tag="LP524_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBL2233850.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20787..22181
                     /gene="uxaC"
                     /locus_tag="LP524_000778"
     CDS             20787..22181
                     /gene="uxaC"
                     /locus_tag="LP524_000778"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBL2233851.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22340..23023
                     /locus_tag="LP524_000779"
     CDS             22340..23023
                     /locus_tag="LP524_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HBL2233852.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23025..23861
                     /locus_tag="LP524_000780"
     CDS             23025..23861
                     /locus_tag="LP524_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBL2233853.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23872..25626
                     /locus_tag="LP524_000781"
     CDS             23872..25626
                     /locus_tag="LP524_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2233854.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25902..26243)
                     /locus_tag="LP524_000782"
     CDS             complement(25902..26243)
                     /locus_tag="LP524_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBL2233855.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26311..28482)
                     /locus_tag="LP524_000783"
     CDS             complement(26311..28482)
                     /locus_tag="LP524_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBL2233856.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGATETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28651..29550
                     /locus_tag="LP524_000784"
     CDS             28651..29550
                     /locus_tag="LP524_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBL2233857.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(29621..30478)
                     /locus_tag="LP524_000785"
     CDS             complement(29621..30478)
                     /locus_tag="LP524_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL2233858.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30463..33165)
                     /locus_tag="LP524_000786"
     CDS             complement(30463..33165)
                     /locus_tag="LP524_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBL2233859.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33178..34467)
                     /locus_tag="LP524_000787"
     CDS             complement(33178..34467)
                     /locus_tag="LP524_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HBL2233860.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34617..35663
                     /locus_tag="LP524_000788"
     CDS             34617..35663
                     /locus_tag="LP524_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL2233861.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35665..37818
                     /locus_tag="LP524_000789"
     CDS             35665..37818
                     /locus_tag="LP524_000789"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HBL2233862.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(38291..39742)
                     /locus_tag="LP524_000790"
     CDS             complement(38291..39742)
                     /locus_tag="LP524_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2233863.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(39739..41463)
                     /locus_tag="LP524_000791"
     CDS             complement(39739..41463)
                     /locus_tag="LP524_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL2233864.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(41456..42070)
                     /locus_tag="LP524_000792"
     CDS             complement(41456..42070)
                     /locus_tag="LP524_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBL2233865.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(42415..43872)
                     /locus_tag="LP524_000793"
     CDS             complement(42415..43872)
                     /locus_tag="LP524_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL2233866.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(43913..44833)
                     /locus_tag="LP524_000794"
     CDS             complement(43913..44833)
                     /locus_tag="LP524_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL2233867.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(44845..45792)
                     /locus_tag="LP524_000795"
     CDS             complement(44845..45792)
                     /locus_tag="LP524_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL2233868.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(46271..46990)
                     /locus_tag="LP524_000796"
     CDS             complement(46271..46990)
                     /locus_tag="LP524_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBL2233869.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(47039..48685)
                     /locus_tag="LP524_000797"
     CDS             complement(47039..48685)
                     /locus_tag="LP524_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL2233870.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            48864..49454
                     /locus_tag="LP524_000798"
     CDS             48864..49454
                     /locus_tag="LP524_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL2233871.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            49546..51051
                     /locus_tag="LP524_000799"
     CDS             49546..51051
                     /locus_tag="LP524_000799"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HBL2233872.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(51135..52487)
                     /gene="celB"
                     /locus_tag="LP524_000800"
     CDS             complement(51135..52487)
                     /gene="celB"
                     /locus_tag="LP524_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBL2233873.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(52487..53005)
                     /locus_tag="LP524_000801"
     CDS             complement(52487..53005)
                     /locus_tag="LP524_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233874.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(53021..53347)
                     /locus_tag="LP524_000802"
     CDS             complement(53021..53347)
                     /locus_tag="LP524_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HBL2233875.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(53360..55339)
                     /locus_tag="LP524_000803"
     CDS             complement(53360..55339)
                     /locus_tag="LP524_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBL2233876.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(55360..55674)
                     /locus_tag="LP524_000804"
     CDS             complement(55360..55674)
                     /locus_tag="LP524_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HBL2233877.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            55842..56135
                     /locus_tag="LP524_000805"
     CDS             55842..56135
                     /locus_tag="LP524_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBL2233878.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(56213..57325)
                     /locus_tag="LP524_000806"
     CDS             complement(56213..57325)
                     /locus_tag="LP524_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL2233879.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            57712..57891
                     /locus_tag="LP524_000807"
     CDS             57712..57891
                     /locus_tag="LP524_000807"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233880.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            58085..58255
                     /locus_tag="LP524_000808"
     CDS             58085..58255
                     /locus_tag="LP524_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL2233881.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(58679..>59257)
                     /locus_tag="LP524_000809"
     CDS             complement(58679..>59257)
                     /locus_tag="LP524_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL2233882.1"
                     /translation="DGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKEN
                     RLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQ
                     LVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLP
                     KTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT        17741 a        12092 c        10356 g        19068 t
ORIGIN      
        1 ctctttttta aaagtttttt tcgatttcgt ttttcgaaat ctttcagcaa ctcatttatc
       61 ataacttatc gtttgttatt tgtcaagaac ttttttgaag tttttttcga tagaatcaag
      121 cttcgcaagt ggttcttttt aacgacttag ttatcttatc acgtctaaaa acatgtgtca
      181 actctttttt ggaattttca taaaatgttt caatgaagcg cgacaacgtt gtttaatata
      241 tcattaaaaa aaataaaggt caagctaaaa tattatttaa gcttgacctt attataattt
      301 caacgattgt caaaagtatt gtttgcagct gtgttgccag tttctataga taaatcacag
      361 cttttgaaac ataagttttg tactgcttcg attcgcccga attcttttgc agttatttgg
      421 caatgagaaa agcagacatc ttctataggc ttttcaggaa atgctactaa atcaaaagct
      481 cgggaaggaa gtgagtaacc tggagaaagg catgcctgta cgtttttgat gtgtatgtct
      541 ttgactttcg tcatttgctt tgattcaggt atttcttcag ccactgcttg ccaactttct
      601 ggcatttgag caaggtcatt gctatttttt ctattgtatt cattatgcca gtccattatc
      661 caagaaaaag gaaattgtac atcaatcata ttcagatttt ctaccctgat attttcgatc
      721 actccccctc tttcttttga agatttcatt cgaataccac agtctgactg aaaaaaatcg
      781 atatcgtgaa tatatacgtc actaactcca gcactaactt cgctaccaat agttacaccg
      841 tatcctgaac ggattttaca tctagcaacc tcgattcttg atgattttcg attgactctt
      901 gcaccattgc catctctgcc agacttaata gcaatacaat catcaccaca ggccaaatca
      961 cattcataga cacgcacatt tgttgaagaa tcaatatcaa tcccatctgt acttggtcca
     1021 tcattgttgc ggatgattac tttttctact aatacatcgt tagaataagt gatttgcaag
     1081 ttccaaaaac ctgatttctg aaaaatcaaa tcacgaacaa caacatgttc tgcatgatac
     1141 agtaaacaag cgcgtggacg tttaatagca taatccgcaa tccaacgaag tcccttttga
     1201 tcgtattcag ctcttgtccc gcttttttga tcttttcccc aatacagctc ccaccaatgg
     1261 tctccttgac cgtcgattat tccttttcct tctataagaa tatctttcgc cgagagaaca
     1321 ttcaaaatcg ctgctggcca tttcatttct acgccggcta ctcgtgtatc gatttctgga
     1381 aatgcttcga tatctgttct ccccttgagg actgcacctt cttcaaagat aagatgactc
     1441 ttatctttta aaaaaagggc tccgactaaa taaatgcctt ttggaataaa tacaggtaag
     1501 tgttctttcg caccttgatc gattgctttt tgcagtatta ttgtatcatc ttgttgagtg
     1561 tcgtttgttt taaagttcaa agcattgata atcccttttt ttgtccgcaa tacgtttctc
     1621 cctatctttc attgattttt ttcaattctt caccaataaa tacagttttt gaacgattca
     1681 gtgggtaacc tactagcaaa atgattgcgg atacaaataa gcagaatgct ggtaacaagt
     1741 ttgccatcgt ataaatcctt tcttgtacta aaactgattg gatggctcca cctgttgtag
     1801 acgattggta accaatgagt gccagcataa atccgcctac cgctcctgca catgcttgtc
     1861 ctacttttct cgcaaaagaa tttactccat atactgttcc atcttctctt aagcctgtct
     1921 tatattgatg ataatcaatc acgtcagtaa tgaatgccca gaccatcaaa ttgaataaac
     1981 cagatcctaa agtagcgata aataaaagaa tcagatatac ccatgcattt gttatatgaa
     2041 ggaaatacat cagcaagtac atacatgaag aagcaagtaa cgcaaatacg cttgcttcct
     2101 ttttaccaaa taattttgtc agctttgtag caaatggtgc taaaactact actgttccgt
     2161 aagtaaataa aagagcaaca gacattgctt gtttattatg gaagtaatca ttgaataaat
     2221 aagtcatatt cgtcccagct aaaatctgat tgatcacaat gaaaatgtca acaatgacta
     2281 aaataatcag tgctcgatta gctaacaaac cttttaccaa ctgagaagca gatacttttt
     2341 cgtttttgac aactcttact ctttcagtag tcaatttaca agttaacata tatgcgacaa
     2401 atgctattgc agcacatccg attgaaatcc aaaagaaatg attccctgaa acgacttgtt
     2461 gtccactctt gttatgggta tacattaaaa tcggaatgaa gaatcctgta gttgatccgc
     2521 caatcgcaga accaatgctt cggaacgtgg acaacgaggt gcggtcatta ggatttgagc
     2581 taattgctgc tgccattgct ccatacggga tattgattgt tgagagaaaa atcccataaa
     2641 caatgtatga aacaaaaata taagcaatct tcaccgtcat tggaagatgt tggacgaatg
     2701 gcaaaaacaa aatgacagtc actaagcaaa atggatactt catttttctg atccaaggaa
     2761 taaatcggcc agtttctgtt aactctgaga catcacataa tcttccaacc atcacatcag
     2821 caaacgcatc caaaattctc gaaatcaaga ataacgtacc tacgattgcc cctgaaacgc
     2881 caagaacatt cgtataataa atcatcaaga aactattgac taaacctaaa ataaaacagt
     2941 tacctagatc accgaacatg tagcctatct tatcggaaat cccaaattcc tttttttctt
     3001 tttttattgt atttgctgta acagcatttt gtgaaagcat ttgaattcct cctacctctt
     3061 ctagtaaacg ttttcaaaat tataactctt tttttcttgt ttttatattc acaatgttgg
     3121 ctttttattg cattatcttg acagataaaa aacaaaaaat aatctaaaaa cattgatgca
     3181 taagattgat aacttttttt acataacaat atatacaaac aaaaaaattc actaactagt
     3241 gaaaataagt tagtgaacct catttattaa agaattaaat tacagatgat cttttccatc
     3301 atacttgaaa aactcaaaat cagcataact tcctcttttt ttatttagat catgcactgc
     3361 tatcccgata aaatttccag taaaccctcc tgagagtata cacatgtttt tttgcggacc
     3421 tactttcttc cattcttcag ttgatacgtc tttcaaatga aactgtgctg tctctttctt
     3481 tacagtgact ttcaaaaaag tcttcttttc tgtgagtgga atcgtccaat cttctatttg
     3541 tgctactccg ttttctgatt ctatcatacg taggcatttt cccttgtcct cattccaagt
     3601 aacataacaa taaatataat tggattcatt taagtacagt accagtccag ccatctgatt
     3661 aaaattatta ggcatgtatt caaatgctgt ctccgcatca aaagtaaaat ccgtttgccg
     3721 aatagctaac aaatgatgtt cgaaggtaga ttgaagagat tcaccggaaa tcagacgtaa
     3781 aaaacctggt ctacattttg tatcacacca atcagcattt ggcaaaatgc gtctggcatt
     3841 ccagttcttc tttagctctc cttcaaatgt atccataaac tgatgatctt tttcttgttg
     3901 tacctcttca tttgtctcaa tcattatttc ttccatcgga gttgtctggc tatctttaag
     3961 tcttaaccaa tcgtcggacg tccaatacac ttcttgaata gcggtttctc ttcccaaaat
     4021 agccgcaccg tttaatggtc tggtacaaag atatgtcatg taccatcttc ctgacggggt
     4081 ttgtactaag ctcccatgtc ctgtacattg caatgtagaa gcaggtttgt cactagctgt
     4141 cagcatagga aaaccaggat caagctcgta aggaccagta atattctttg ctcggcaaac
     4201 tgtaactgaa tgtcctcttc ctgtaccgcc ttctgccgtc atcaaataat aatacccgtt
     4261 acgaaaatac aaatgtggtg cctccgtttt cgctaattct gtacctgcaa atattttata
     4321 gatgggacca tcaagcttct tcttctctgc atcatattct tgtaatacga taccgctgga
     4381 tttattcggt gtgtccagac gataatccca tagttcattg acaagccatt tttttcccgt
     4441 ttcaagatca tggaacaaag aaggatcaaa gccgttacta tttaaataaa ttggttcgga
     4501 ccaaggacct gtgatctcag atgcagtgat caaataattg tggacatctt taaacggacg
     4561 gcctgcactt ttgacatctg tgtaaacaag ataaaacaat ccatcagcat aactgatctg
     4621 tggcgcccat atacttccat ttgtaggatt tccttgcaaa gatacttgag tggtcaaaat
     4681 atccgttgta tgtgaccagt tgactaaatt tttcgactca tatactcgga ttcctggaag
     4741 ccattcaaaa gaggagacaa taatgtaata ccgctctttc actcttaata tattaggatc
     4801 tggattgaat cctcttaaaa taggattttt cactttgata gtctgcatga ttcaggctcc
     4861 ttttctcatt cttactcgat ataaactgaa tgccccagct gcgaaataaa aaaatgcaaa
     4921 aggaaacgca ataaactgag tgccaatcaa caatatgaat cctgttataa aatacaaacc
     4981 agtggcagtt gttaagtatt ttcctttagc agtcaaaaac aatgctactg ctaaaaaagc
     5041 aagtgaaata aacaacacaa cggcaaacag accagtcatg tcctgtaata atccaaatgt
     5101 attttctaaa gacaaagttg aatgatctac agcaagcgac tgataacttt ttttaaactc
     5161 tgacgcagac atattactta aagtaactgc taacccgcca aagaaaatca aagatagact
     5221 gccaccgacc attcctagca atagttccca ttttcttttc atttctgaac cccctgcttt
     5281 tctaaatgta tccattcagg tcttttgatc gatgtttcat aattgttatt acgttcagag
     5341 aatttgacag tctgttcatt tttcttgtca tcccaattat cccaaccagt tgggtgaaca
     5401 tgcccaccta tcttacacgc ttcaaataag acatttgcat acggacgcca cggtcttcct
     5461 aaatagacgt ttctagtatc aggttctgca gtcaaggtac agctttcaaa acgataccct
     5521 gttttttgat ttttcggcgt agatgctgct gtgatgtatc caggattttt actggctaag
     5581 ctatggatat gacactgatg aaaaacggca tctgccccac caaatatgaa atccaccgtt
     5641 ccttcaatcc agcaatattc ataggtctga cgacaatact caggattggc agaagccggt
     5701 gtacaaaaag gagtaccatc tttttgcaaa tcaggcaacg gacccgtgca taaggtatcc
     5761 tgataaccag atagtcgaca gttttttaaa gatacgcgat ctcctgaatt gaataaagca
     5821 acagcctgtc ctaccttttc tcctggacct gctgtatttt cgatgtgaag attttctaat
     5881 acgacattcg atccttctaa gaaaaaagta gcacttcgga aagtccctaa ctggctaccg
     5941 tcaagatact cctgttttgc aaaacgatta ccggagataa ctactgttcc tgctcccaca
     6001 agtttaaaat tggataatct gctttctata tattcatcat atcttccttc ttttactatc
     6061 aagacctttg gatggctatc cggcaggcct gccaaataat cgattccttc ttggatagtt
     6121 tcaaaatccg tcggggtatc tatagcattc tttccaatta tcagtttgtc tttcatcctc
     6181 ataaatgctc ccttatgaat tgtttccaaa tatttctcat ttcttgtgtt tccatatgac
     6241 cgcctgttaa agaaaaagaa gaaaagtttt ctggactacc cattgattga tactttttgt
     6301 ccagcccttt tctcagcttc agtacgcctt cgtccaagca catcgtatcg ttttttccaa
     6361 ctaagctaag acgagggcgc ggagcgattt tttcttggat tgctaacgtt gtataagctg
     6421 tcaatagatt tggcacatag taataaaacc catgatgatc taggcggcga tgtagcaaaa
     6481 gtatttcgat gtccacctgt gccgctagat caatacaaac tttgacacga gaatccaatg
     6541 ctgtcaccca ccagcttaat aagcctccca tagaaagacc aatcgttgcg agccgttgag
     6601 tatctgtgtc ttcccttgtc tccaaataat cgatcaagct gatcacatca taaatacgca
     6661 ttccccataa cgtttttcct gttaataaaa attgtttgaa caactcgctt tcagaaatac
     6721 ctccgcgttc ttcaaatccc caagcatcaa tcgaccaaac agcatatccc attcctgtca
     6781 gtacttctgc aaaagatgga gaagctaagt aaggagcacc atggatcaat tcacttttcc
     6841 cgactgaaaa atctccgccg tgagaatgca ggtaaaggac cggcggaaac ggtccttctt
     6901 tttctggata agcatagagg gctggtactg tttccaattc atttaagtac aattgatagt
     6961 attcaagcat aaaaccgttt tgtttttctc ggtataatcg ttttccacta ggggtatgtc
     7021 tgctagatgg agcacctaaa aaatcattca actgcataag caaagttttt acgaacaacc
     7081 agttcatcca tattttctga ttgatttgat tggaatttct caatactgat ttctttagta
     7141 ttttctaata gaaatgctgg tccctcgtga ttttcaatcg acatctggtt aaaggaaacc
     7201 ttacccgcaa agccaatata aaatccttgt ctgctcattt cctctattcc tgtcatcata
     7261 gcaggaacac cgggaactgc atcttgggcc atcgagacat cgatattgtc aaatgtgatt
     7321 tcttgtatga attgttccgc taatccataa ataaaacctg cagaagcatg gacattcctt
     7381 gccgtgatat tcgaaaaatg gatccttctg aatgctggtg tccgttcatc tatcggataa
     7441 gccttttttt cccatacata cggttctttc cctcgcggac cacagaaata ataaagattc
     7501 agtataaatg gacacatcac attatccatg actatattgg atactcgaat atcttcaacg
     7561 atcccgcctc gacctcgtct agatttcaat cgtattcctc gatcagtttc ttggaagata
     7621 caatttgaaa tggtaatatt acgaatacta ccactcattt cacttcctaa tacgacaccg
     7681 ccatgtccat ggaccatcgt gcaatttgtg atcgtgatat tttcgcaagc gatcctttcg
     7741 tacgtatctt cagtccctgc cttgattgca atacagtcat ctcccacgtc aatatggcaa
     7801 ttactaatac gcacattttt gcatgattct ggatcaattc cgtctgtatt tggtgaatcc
     7861 gctgggttca agatcgtcaa gttatcaaac gttgcattac tgcaaagaat cgggttgatc
     7921 gtccaacttg gtgactggat caatttaata tctttcacag taattcgatg acagttatgg
     7981 aaactcatca atttaggacg aggataagcg agattatctg gttcatttct aaaagtatgc
     8041 caccattttt ttccatttcc atctaatgta ccgaaaccag tgacagagat gttctctaca
     8101 ttttgagcat agatacagga agcataaact tttcgatgga ctccttccca acgtgaatga
     8161 acaacaggat aatcttttgg atcgtcagag aatttcagaa ccgctcctgc gcttaaatga
     8221 agttcgacat tgctttttaa aaataaagct cctgttagaa attcacctgc tggaacaact
     8281 acggtcccgc ctccgtcact tgctgcagca tcaatggctt gttgtattgc ttcagtattc
     8341 aattcatcaa ttgatgcgcc aaatgtcaaa atatcataca tatttataat gcctccactt
     8401 caatcaatgc ttgcaagaat gcaccaacgc ctttttgatc attacaaata attggttcac
     8461 tgatgtagta agcatatgaa ccatctcgct gatctgctcc gcctaaccct gctacttgac
     8521 agtttttatt caagttcagc cagccttctt ttgtttccag aacaaattca tcaagtaatc
     8581 cttgatgtga tttcttcact tgtggaagcc attcttcttt gttcagcagt cccaaacgta
     8641 ttcctttagc cattgcgtaa acaaacatac tactgccgga agcttccaga taatttcctt
     8701 tccgttttgt ttctgttgtt acttgatacc atgtcccgct gttttcttct tgatatggaa
     8761 gtaatgcagc cagtgtcttt tctagaattt ctttcagcaa agaacaatct gtttcttcat
     8821 gaagtaattc ataggtatct gctgcagcca tcaaatacca gccaatcgat cggctccaaa
     8881 aatgatggga tagacctgtt tttggatcag cccatggttg gaccttcttt tcatcccatg
     8941 catgaatcag caaatttgtt tttggatcga atagatgttt ataactgatt tcaaactgtc
     9001 tcaaaacatc ttggtaatct ttttcttcac taaaattctg gataaattca gcataaaaag
     9061 gagaacccat atataagcca tccaaccaaa tttgataagg ataaatttct ttatgccaaa
     9121 aagctccttc ggatgtgcgt ggatggtttg ccaactgctg ggaaaggagt tctgctgctt
     9181 ttttatattt ttcttcgcct gtttctttgt acaataaaaa gaaaagcttt cctgtattta
     9241 catggtcgat attgtattct tcaacgctgt agccacggat cgttccatct tcttgcacga
     9301 aatagtcgat attgtctttt atgaaatcaa aataaatttt ttcttgggtt cgttcccaaa
     9361 gtaattggaa ccctttaagt acgacgccat agtcataaga ccattttcct ttataccact
     9421 tttcttcata taagcgaggt gttctttcca tgatactttt tgctaatttc tttgaccagt
     9481 tttccattat ttgctctcct ttaaaaatta aaataggatt tgggacaaat accaagaagt
     9541 atttgtccca atcgtctata ttactgtgcc gctgctttgt atgcttcctc gtactcttgt
     9601 gtgattcgat cgcctccttg tgctttccag tcttctacag cttttttgaa tccatcttcg
     9661 tcaagctgtc ccatgatgta ttgcaccgta gcatcaatga cgattttttc aatttctgat
     9721 cctacttcat tgttcgtcgg tgaatccaat ggcacagaag gatccattac agcaaattct
     9781 tcattttctt tgatcaattg attagagaat tctttttcag ggtctgtatc tttgaaagag
     9841 aacgtcactt cactaggacg agagcttgat aacggttgca cctcttgttg ccaaagatcc
     9901 atatcgataa attcgataga accatcatca ttcaattcat aatgcgttcc ttcgataccg
     9961 ccagtcatca atttatacac atcttcttct aaaagatcat caacaaattg aagtaatttc
    10021 ttcaattgtg cttcgtcttt tacttgagat ttgggaaatg ctagcaagcc gccaatacca
    10081 tttccttctg accaaacatg ataaccgccg ccttcaccat ctgagatctt gtttacaggc
    10141 actaattcca tatcatcttg aataccttga cccatcgtct tcagattctt gatatccacc
    10201 attccagtgt agattccagt tcttccttgt gcaaattgtt cttgctgatc agtcttttgg
    10261 gtaactgcaa agtcttgtgc caaatagcca ttctcaaata gatctcttga aaaattcaaa
    10321 gctttcatat actcttctgt atcaaattct ggagtgaatt tgccattatc gtctacagcc
    10381 cagccatttg gtgctccaaa ataagaagtg aaaagtttaa agctggtata acgaatatca
    10441 ctaggcgcac gatcgccaaa accaactgtg tcgtccacac catttccatc aggatcgttt
    10501 tctgtaaatt gtttagctac ttcataaagc tcatccatgg tagttggaac atctagtcct
    10561 agattatcca accagtcttt tcgaatgact aagccagcac gagcgtagtc tttttgtatc
    10621 ggtacgccgt acaaggcacc gtcgatactc gcagcttcga ttctatcttc agaaatttga
    10681 cttaaatttt tatagtcttt taaatattct gatacatccc agaatacgcc tgattttaac
    10741 gcattgcgga cagaagagtt tttcaacatc gcaatttgaa gtgtaacgat atctcctagt
    10801 tcatcagaag ccaatgcagt tgttaatctt tcttcttttg aagcatcagg aatccagttg
    10861 aatgtcagtt ccgtatcggt tttttcttcg atcaagttca caatcgtgtc tgtaggcggt
    10921 gaagcatgat gcaaaatatt taaccaagtg attttagctt ttccatcttc tgtctctgaa
    10981 gaactgttac ttgaactgct gccgcatgcg gcaagtgcta gaattcctgc aaaactaaga
    11041 cctaatttta cgagcgttgc ttttttcata aattttctcc ttcactgttt taataatatt
    11101 tttgttttct tctgtttttt ctgtatatga gttcttccat tccaaatggc aaaatataga
    11161 atttgctgtc cacgtgactg cggaaaagaa gagactgcca ccgataaagg ttaaaacacc
    11221 tggcattgtt tggatgatca tcagataaat ccctaccata cttaatatca acactaatga
    11281 gtaatgaaga tgtgagaatc cgaataaaaa tgccattttc attttgaaaa aatatccttt
    11341 ccaatcataa tgagccatga ttgggaaaat atagagtgct gagagcacat atatgaacgc
    11401 agcaatatac aaagcgatcc taagatacca attgtgtatg aaagaaagat ccacaagtaa
    11461 aacaatcccg atggcgccat atatccagct catcagcaag gtttcacgat agttttcttt
    11521 aaaatatgtg aagtacgttt tcgtcaaagg aaacgtttct tttgagcgca gccattttct
    11581 aatcactgca accatcgcat acgatgcagg accgaaagta aaaactccca aacctgcgat
    11641 gactaaaccg ccccacagaa aattcaaata aaccaactgt aaaatacgat ttagccttcc
    11701 gtttatttta tctaatgact gcagcatatc tttccctctc tttctatctt agtaaacgcc
    11761 gtcttctcct agctttttgg caatcttatt agctaaaatt accataaaca atcctacgac
    11821 ccctttaaac aatcccattg ctgtactgaa acttaactgt ccattcttca atccagctgt
    11881 ataaatatag gtgtcgaaaa tttctgcaac gcttcgattc aatgaattca ataacaagta
    11941 catatgctcg aaccctaagt ctaatgtctg accgattttt agaatcagca aggtaatgat
    12001 tactggacga attgccggca atgtaacatg ccatgttttg cgtaagcgtc ctgctccatc
    12061 catttctgca gcttcataca actgtgtatc gactgcagtt atcgctgaaa ggtagatgat
    12121 cgtagaccag cccaactctt tccagatcac ttgtccgata tatactgtgc gtgtccattc
    12181 aggggatgtt agaaaactga ttttttcaaa tcccattgtt gcaaggaatt cattcaatac
    12241 ccctccgtca acattcaaaa atacataagt aatcgaaaca atgattaccc aagacatgaa
    12301 gtgagggatg taaatgatcg tttgaatccc gtttttcaat cgtttgttcg ttacttcatt
    12361 taatagtaaa gacaaaatga ttggcatcgg aaaaaacacg aagatattta atccgaacaa
    12421 gatcaacgta tttcttaata atgtaaagaa tgtcggttct gtaaacaaac gaatgaaatg
    12481 tttgaaaccg acccaaggac ttcccattat tcctaaaaat ggttgataat cttgaaaagc
    12541 aataacgagt ccccccatag gaagatacct aaaaatcaag aaataaagga ctcccggcag
    12601 aatcatcaca tataaaaatt tattcgtttt gattcctgca attatttttt ttcgtcttct
    12661 gagcttttct tcttttttcg atagcgtcag ttgaaatggt tcttgtttct ttacactttc
    12721 cataagcccc tccttagttt tgtgaatgtt tgctgcttat gacttcatga tagataacta
    12781 tctgaattac gtatataatc atttttaccg taagtaaaaa cgcttacata gcaatgattt
    12841 ttgtgctata caaaacgata aagtgtattt tttcacaaat aaactacgca tagcaaacct
    12901 tctatttttt tctatctgac acttagcata ggaaatgatt atatacgtaa tctttcaccg
    12961 atgataaatt gaacatatag aaacagaaac atttaattta ttttgaggag gagcctatct
    13021 atatgaaaaa gaaagtctct ataggagaaa gaagttttac acttttcaac aatacgtttc
    13081 taattttgct agctttgatc tgtatcgttc cttttttaaa tatcatcgct acatcttttg
    13141 cttctacaca agaagtcgtc gcgaaaaagt ttatcctttt tcctactacc ttttctttag
    13201 acgcgtatcg ctacattctt tctacgccga ccatttttag agcacttgct gtttcgattg
    13261 gtgttacagg tgtcggaacg atcgtcagta tgtgtgcaac ttcactgatg gcctacggtc
    13321 tttccagaaa gtatttgttt ggtcgaggtt tcgtcaactt tcttgttgta ttttctatgt
    13381 tgttcagcgg ggggatgatt ccaacgttct tagttgttcg ttctctaggg ctggtcaatt
    13441 cttactgggc catgatcctg cctgtggctg ttaatgcaat gaatatgatc attatgagaa
    13501 actttttcca agccttgccg gatagtttgg aagaatcagc aaaaatggat ggttgcacag
    13561 attttggtgt atttttcaaa attatgctgc cattagcgtt accgtctatt gctactatct
    13621 cgctttttta tgctgttact tactggaaca cttatatgac tgctattttg tatatcaacg
    13681 attcatctaa atggccgata cagattcttt tacgccaaat cgtcattgta tcaagtggga
    13741 tgcaagcaga aagtagtgcc gttgatgtga ttccaccagc acaaacagtt aaaatggctg
    13801 ttatcgttat tgcaacagtg cctatgctga ttgtttatcc ttttgttcag aaatattttg
    13861 tcaaaggagc actcgtaggt tctgtcaaag ggtgattatg tactgaacgc aaaatggtag
    13921 ttgtgtcaga agtggtcagc ttcaagaaat aagaaggcat ttacgaaaat tgcttcttaa
    13981 atttttgtga aatttcggct tatttttcga agaatctact cctggaacac cgtgtatcag
    14041 aggttgtgaa agagacgttc agccttgagc gatgataaaa actctcacaa acattgcgac
    14101 gagtaaccca caggagcaat aagaagcgat tttgaaaaat agcttcccat atttcacaaa
    14161 attatgcctt attgtcgata gcctgtctct tgaacgccat ttattcgtaa aaaagaactg
    14221 tgacaagact tttgtcacag ttctttttgc gtgcttagcc ttgaaattta actatccata
    14281 aatacaaata ctagggttat tctaacttct tttattgctt gaatcagtcc acaagaaaaa
    14341 cagcctcttg attcacaaat atttttttcg atattctccc ggcgtaatcg attccttctt
    14401 tttaaagaaa cgaataaaat tttgcggatt tcgatattgc aatctttctg caatctcttt
    14461 tacggagagg ttagtctctt tcaaccattt ttttgctacc tctaaacgat gattttgaac
    14521 aaaatcacca aatttttctc ctgtttcctt tttaaataca ttacttaaat agttagggtt
    14581 ataatgcaac ctatcagcga tcacatctaa agacagatca cgatcatatt ctgtgtagac
    14641 gatatgcata atattctccg acaaattttt aaattcatga tcagtgattg cttgagagca
    14701 ttctgtgatt ggcagaacta attgttctaa aacaagtttt tctagttctt tgggatgata
    14761 attgttcagt gcttttacat aaatttgttt catgttatca aagttttttg aatctactcc
    14821 caataactgt cctaattgaa cgagttcatt gatcaagcgg accgaaacta cttcccgact
    14881 caaaggattt ttattttgtg caaacaatgc atcaatcaat tcatgtacta aattcttcac
    14941 ttcctgattt ccagaacgaa tggcttcaaa caatcgattt tgttcttcta tcggatatct
    15001 aataagtgtt ttttcatgag cagcaggaac gatttcctga taaaaaataa ttgaacttgg
    15061 accagtattt acacgataat acagggcttc ttttgcccga tcaaccgatt gcttgctttc
    15121 attcagcgtt tcgaaccggt tactgatgcc tatactgata gttaaattca agtattcttt
    15181 tacacgtttt tgaatgagtc ggcaatactc catgactttt ttgcttgctt ctgcatcatc
    15241 tttatccatt cgataaatag tggcttgcat ttcttcattc aaaacgatcg gcagcattcg
    15301 attttccttt ggcacaattt ccgtgatcat gttgttgatc gctaaaagta gcaaatcacg
    15361 ctccccaccg tgactatctt ctaacagatc gatttgaacc aaagctgtat aatagcagtc
    15421 attttgagaa ctataaccaa attgctgcaa ccgctgatct agttctcgcc tttctactcg
    15481 atttctaaat aaactaagta caaatagcgt ttccagctgt ggcttttggg taactaagtg
    15541 tgcacttaac gcttcttttt ctcctatgat ccgactgact gattgagcga ccaaatctag
    15601 ttctttccca ctgaatcctt ttgatttaag attcagacgt tcctgtattt gagaaatcgg
    15661 acgggcaaat ctgtccgaaa ataagtagga aagaagacca atgagtccta ccagtgttaa
    15721 agacactgca actagcccga ttctcagatt gcggatcatt gcgccaattg cactagagtc
    15781 aacttctaat ctgtaaatcc aattattata atctgatttc agtactatat acgttttatc
    15841 tttttctctt atcactttca gctgattgta atctagatcc acgtccgtta atacagttgg
    15901 ttgctcttta ttttttactg tctgagtagt aaaaaggttg ccaacttcgt tttcaataga
    15961 aagtatgtct tctccacttt caccaacaac ttgttctatt gtatgtttct ctatcgtagc
    16021 aattcccaat gcatattttt ctttttcaaa cattggcaaa gaaatcacca tttcgattcc
    16081 tttgtttttc ttttgccaaa ataaattatt tttgttattt atgtacttct gttgatactt
    16141 ttcaatttcc tcttcggtca gttgactcaa cgatccattg attactcccc atttaccagc
    16201 caaactgact aaatcataat tattatcatc cataacaatc gtttctataa aatttagttc
    16261 tttcctgatt tcagaataag tggagtaatc tgtatatgtc agcggatgct tgaatatgtc
    16321 cgagaaattt ttggtcgaac catacgtaga aaacgaatat tcaatcgttc taattttcca
    16381 ttcaacgtta gttttaactt gcctgagcaa attctttttg tctgttaaat aactcgattc
    16441 gatcgattca taggtattct ggtaaatgaa taaactaaat cctatgacaa gtacggctcc
    16501 taataaaaac atcggtaaaa atattttata aaatactgta cttttcttca ttttcttcct
    16561 ctcctccttt taaaataaaa ggctttcatt tttctgtaaa attgatactc atctaattaa
    16621 atgagcattc cccgctttca agaatagcat aattaaaaat aaaaatccaa gtaattttta
    16681 acgtgtgcaa tcttttcata tcaaagatct attgacttat cgtagttatc gtaatatgat
    16741 gaatgcgtta acaaaaagtg aaaaagtaag gaaaatgatg gaatggtaac gatcaaagat
    16801 gtcgctaaag cagctggcgt ttctcatacg acagtttctc gtgctttgaa taacagcagt
    16861 ttaataaaaa atgaaacaaa acaaaaaata cgacaactag ctaaggaaat gaattattcg
    16921 ccaaactata gtgcaaaagg tttagtgaat caaaaaaaat atctaatcgg actatttttc
    16981 tcaagtttgc ataaaggaac ttcatcaaat ttcctagcag attccattgc aggcatccat
    17041 tcagtattag acacaaatta cagtttatct gttgaaagta tcgatgttgt tgatttaaca
    17101 gaaatcaacc tgcaacgcta cgatggactg atcgttatga gtcaaagtga tgatgatcag
    17161 cctttcatcg actatctcaa agaaaaagaa tttcctttcg ttgttgtcaa tcgtcacatc
    17221 aatgatccag cggttatcaa cgtcactgcc gatgatgcgg ccggtgttat gcaggcagta
    17281 gattacggta ttggatgcgg acatacgaag atcgcttata ttggcggaaa gaaaaatttt
    17341 cgttcgacaa aagaacggga aaaaggggta ttgaaaagtc taaaaaaagc gagcatccct
    17401 gtcaatccag cctatttttt aacaggagat tacagcttag aaagcggcct ccaaaacatg
    17461 gaatacctat tatcactttc tgacctgcct actctggttt tttgcggcaa cgacgacata
    17521 gcgattggtg cattgagagc tgtacattta gctggcctcc gtgttcccca gaacatttct
    17581 ctgatcgggt tcgatgattc tccagtcgtc tcctatctca atccgccgct taccaccgta
    17641 cgtaagcctt tgaaagaaat gtgtcaagaa ggaacaaagc ttttactaga tttgatcgaa
    17701 ggcaggacga tcgaacaatc taaaattgag ctctccacct tgttgatcct tcgtgagtcg
    17761 atcaattact tgtaaactac aagtaatttt atataaaaat gttaacgtgt gcataacaca
    17821 gaaaggaagt ttatatgaaa aaactgacaa aagaattagt gaaaaaaaac gaagcacctg
    17881 taaaagttct tcagtttggt gaaggcaatt ttatgcgtgg atttattgat tggcagattc
    17941 agcagctgaa taaccaacaa ttgtttaaag gtaatgttgc aatcattcaa cctttagaac
    18001 aaggtcttgg aaatatgatg aaccagcaag ataatttata tacggtcatt ttacaaggac
    18061 tcagcaatgg cgaaatcgtt gatacttccg agattatcac cgtagtagaa caaatgatca
    18121 atccttatga tgaatgggat gcttatctat ctctagcaga aaatgacgac ttggcctata
    18181 tcgtctccaa tacaacagag gcaggaatcc aattcgtgga gactgataat ttaacagata
    18241 ctcccccaca gagcctcccg ggcaaactga cagccttttt ataccgacgt ttcaaattaa
    18301 acaaagctgg ctttaccatc attccttgcg aactgattga cagaaacggc gaacgtctaa
    18361 aagaaatcat tttacgctac gcagaaaaat gggatttaga acctgctttt gttgagtggt
    18421 tagaaaaaga aaatgttttc tgttgtagtt tagttgatcg catcgtccca ggttatcctc
    18481 gcgaagatgc aggtgttttc aatcaacggc ataactatga agatcaattg atggtcaaag
    18541 cagaaccatt catgctttgg gtgatcgaag ggcctaaaga attggagaat caactaccat
    18601 tcaaacaggc tggcttgaat gtcatcatca cagatgatat gactccttat cgtgaacgta
    18661 aggtgcattt gcttaatgga ccgcatacag cgatggtccc tcttgctttg ttggcggaat
    18721 tggaaactgt agaagaagta atgaaagatc ctttattctc cgcatatgtt gatcagttat
    18781 tcaatctaga attgattcct atgctttctt tacctaaaga tgaattagcc atttatgctg
    18841 accaaatcaa agaacgtttc ttgaatcctt ttgcccatca caaattagaa gcgatttcat
    18901 taaatagtgt ttcaaagttc agcacccgtc tgcttccagt gttcaagaag tatatcgaag
    18961 aacaaaatca ggtaccaccg ttaatcactg tttcactagc tgcgctactt cttatgtatc
    19021 gaggcgatca agtaaagcca catgatgatg aaaagacgat aagcgaattt actgatgctt
    19081 ggtctgataa tggaacagct attcctcgat tattgcaaaa cgctgcgctt tggggagaag
    19141 atttgtctca aataccgaat gtgacagata cagttcaaga aatagctgca aagatcgatc
    19201 aaaaaggtgt ccggtcagta ttacaagaaa tgaaaggaac gatggtccat gcttgaaacc
    19261 ctgcaaccaa caatgaaatt aaatcgacga gacagtgtgg tcgtcgcatt aacccctatt
    19321 ccaaaacaaa cacaactgac gattgatcag caaacgatca ccactcttga agatatcccg
    19381 caaggtcata agattgcatt gggtgatttg aaagctggag acaacgtgat caaatatggc
    19441 tatccaatcg gtcatgtgac gacagacgta actgccggtc aatggctgca cactcataat
    19501 gtaaaaacca atctttctgg tgaattggat tatcgatatg aaaaagatgt ccatccaagt
    19561 cattaccttt ttgagaaccg aacatttcaa ggttacttgc gaaaaaatgg aaaagtaggg
    19621 attcgtaatg atttgttttt agtacctacc gttgggtgtg tgaatggcat tgctgaactg
    19681 atcgtcaaac agttcaagga aaaacatcca gatctgggac agtttgacca tattacgatt
    19741 ttaaagcatc cttatggctg ttcgcagctt ggaaaagatc atcaaaatac tcgagaaatt
    19801 ttagcggacg cagtaaatca tcccaatgcc ggaggagttt tagtatttgg attaggttgt
    19861 gagaacaata cagttcctga attcaaaaaa attttaggtg aatatgatga agaacgagtg
    19921 aaatttctag tggctcaaga tgtttacgat gagatcgaac aaggtgtggc tctattagaa
    19981 gagcttttaa cagcggcgga aaatgaccag cgcatatcag tcccgctctc taagttgaat
    20041 gtcggtttga aatgcggcgg ttctgatggt ctatcgggaa taactgctaa tcctttactt
    20101 ggtgcatttt ctgactactt gattgctcaa ggaggcagca cgatcttaac agaagtgcca
    20161 gaaatgtttg gcgcagaaca agtattgatg gcacgagcgg aaaatgaaga agtttttgac
    20221 tcaatcgttg acttgatcaa tgacttcaaa cagtatttct tgtcttacgg ggaaccagtt
    20281 tacgaaaacc catctcctgg taataaagca ggtgggatca ctacattaga agataagtct
    20341 ttaggctgca cgcaaaaatc aggaagctct cctgtcgttg atgtgctaca atatggcgaa
    20401 aaaatcagaa aaccagggct tagtttattg caagctcccg ggaatgatct tgtagctgcc
    20461 tctgctcttg cttcatccga ttgccagttg gtcttgttta ccacaggaag agggactccg
    20521 tttggctcat atgtcccaac ggtcaaggta tctacgaata caactctttt tgaacgcaaa
    20581 ggtcattgga tggattttaa tgcaggcgtc ttattagaac aaccaatgga agtaatctta
    20641 gaatcgtttg ttcaaaaaat catcgctgta gctagtggag aagaaacgaa aaatgaacaa
    20701 aatgacgttc gagaaattgc catttttaaa aacggagtaa cactttgatt ttcataaaac
    20761 agtataaaaa gaaagaagga agatttatgt ttttatctga tgatttttta ttaaaagacg
    20821 attgggcgaa aaagctttac cattcctacg caaaaaacat gcccattatt gattatcact
    20881 gtcacttaga tccaaaagaa atttacgaaa acaacaattt cagcaatctg acagaagctt
    20941 ggttaggagg cgatcattac aaatggcgct tgatgcgagc ttgcggcgtg cctgaagaaa
    21001 aaatcacagg aaatgcttct gattttgaaa aatttttagc ttggtgtcaa accgtaccaa
    21061 aaattatcgg aaatccgctt tacagttggt cacatcttga attgaagcgt ttcttcggta
    21121 ttgacttgtt gataaatgaa gaaaatgcag agaaaatttg gacacaagca aatgaaattt
    21181 tggcagctcc tgattttcgc cgtcgggaaa tcgcaaaaaa atcgaatgta gaagtgattt
    21241 gtacaacaga tgatcccgca gacgacttgc actatcatca gttgcttgct catgaagaac
    21301 cagatttgaa ggttctacct tcattccgtc cagataaagc actcaatatt gaaaaagaag
    21361 gatttaatgc atgggtcaca ctactggaag aagcagctga ccaaacgatc accacctatc
    21421 aagaactgat agaagtctta gggcagcgga tagaatactt ccatcaacat ggatgtcgtg
    21481 tttccgacca cgctttagac atcttacgct ataaagaagc ggatgaaaca gtattagaag
    21541 agatttttca aaaagcacga gctaacaaac tattatcttc agatgaaatc gatgcttatc
    21601 gtacagaaac actgatacat ttgacccact tctatcattc tcataattgg acgatgcagc
    21661 tgcacatcca tgcttatcgt aactgtaata cacaaatgtt ggaaaaatta gggcccgata
    21721 ccggctatga tggaatgaat gatctttctt tgactattcc tctgcaaaaa ttattgaacc
    21781 gtgcagaaga aacggatcag ttgccaaaga cgatccttta ttcattgaat ccgaatgact
    21841 atcctgcgat tttagctttg atgggctgtt tccaaaaaga acagaatgga aaactgcagt
    21901 taggttctgg atggtggtac aacgatactc gagctggtat gcgcgatcaa ttgacacaat
    21961 ttgctgatgg tagtgctttg gggaattttg tcggcatgct gacagattca agaagtttcc
    22021 tgtcctacac tagacatgaa tatttccgtc gtgttctttg tgaattttta ggcgaaatgg
    22081 tcatgcgagg agaagcacct gaagatgaac aacttttagg aagtctagtt caagcgatta
    22141 gttatacaaa tgctaaaaat tactttggct tttttgccta attgattaat agtaacagta
    22201 acgaatcaaa aacaggatag tgaaataacg gaatccgttt atccacgttt tagagaagaa
    22261 gacggaccaa atttgctccg tcttcttctc ttgctgttct gcttcattgc ctataaacct
    22321 ttatgaaaga agagataaca tgcctactat ccatattgcc ggtgattcta ctgcggcaga
    22381 aaaagatgaa aacaagcgtc ccgagacagg ttggggagaa aaaatctctg cttatttttc
    22441 acccgagatc cagatcaata accaagcaaa gaatggacgc agcaccaaat catttatcca
    22501 ggaaggaagg ttagcccgtc tagcttcaca gtttcagccg aatgatttct tatttgtaca
    22561 atttggacat aacgaccaga aaatccaaga agaaagaggt acgcttccct tcggtagcta
    22621 tttaaaaaat ctgcagatat tttttgaatg cgcaaaaaaa gcgaacgtcc aaatggtctt
    22681 actgacatct gtgacgcgac gagattactt agaaaacgga acattgaatc cagatatact
    22741 tggagactat ccaaaagcga tgcgtgcatt tgctgagaaa caccatattc ctcttttaga
    22801 tgtgttctca agatcacaag aattgttcca gaccttctca aaagaagaga caaaaaaatt
    22861 ctacctgcat cttatacctg atacttgtaa aaattaccca gaaggactaa aagacaatac
    22921 ccactttagt ccagaaggcg ctgataaagt tgcccgaata attgttgaat tgataaaaga
    22981 atcccgtctg cctcttgcta attatttaca aaaaggagtt tgagatgttt ccattaaatt
    23041 taggcatccg tgcccacgat ttagatgttc gaaatcgtga agaaatcgtt caaaaagtat
    23101 cacagcttca cttgtcccat atccaatttg caccacaaaa agcatttcct gaacaactta
    23161 aattaactga catgacactt gggagtgctg cctattttgg tgaatacttc caaaaaaaca
    23221 atatcgaact ttccatttta ggatgctata tcaacctatc cagcaaaaat caagaagttc
    23281 gtgcacaagc attggcaact ttcaaacagc aaattgctct gtgctctgcc tatcaagcca
    23341 agatgattgc tacggaaacc ggtagtgtct ctgtcggtta cacaaaagaa aattttactg
    23401 aagaagctta tcagatcgcc agaaattcta tcattgaatt agtcgctcat gcagaaaatt
    23461 ttggcgtaac tgttgctgtt gaagcaggga tcaatcatcc aatctataac tatcagttag
    23521 ctaaacgttt aatcgatgaa gtagcttcac caaatttaaa aatcatttta gattgtgcga
    23581 atttgatgca taaagaaaat catgctgatc aggaaaaagt gatccgaggt gccttggaca
    23641 atttacatgg ttatattaca gcgcttcatc tcaaagacta tatcatggaa aatggaaaga
    23701 tccgcatggt acctgttggc aagggctgtt tagatttccg cccggtactg cattatatca
    23761 aatatgagcg tcctctaatg tacgccactc ttgaagctac tcctgagatt tatgttcctg
    23821 aagcaataga acacttgcag tttatttacc aagattgtta aggagaagat tatggaggaa
    23881 cgaatacaga aaagttatca aaaatatcat gcaaatgctt ttcagacatt gctgcatctt
    23941 tatcagggaa attattggcg ttttgccatg tcaggactct tttttattat caaacatctt
    24001 ccatcttggt taatgccgat cgctattgcc aatgtaatca atgcagcttc atcgaaagat
    24061 tcttctcagt tgcaggccat ttatttcaac gcttttttga tggcttgtct gattggtcaa
    24121 aatatattga cgaattattt tcatgtaaaa taccatagca cttcgatccg tcaagtagaa
    24181 gctggactaa gagcgaccct tatcaagaaa atccaagaac tatctattcc ttatcaaaaa
    24241 gaattgcaat ctggacgaat ccagtcaaag atcatccgtg atgtagaagc tatccaaacg
    24301 ttgtcctctc aagtatttgt cagcggatta aatatcgctg tcaatttatt cgtagcttta
    24361 ggtatcacgc tttataaaag tcctgttgtt ttcatgtttt tcttattgac tgttcctctc
    24421 gccgcattcg ttactctctt tttccgtaaa aggatcagac agtccaactc agacttcagg
    24481 caagaaatgg aagagacttc tgcaaaagtg atcgaagtga tcgaattagc accgattgct
    24541 cgagctcatg ggttagaaaa acacgaaaca actcgcttaa ataagcattt tactcaaatc
    24601 tatgaaaaag gattccactt agatatcctg caatctgttt tcggatcagt cagctgggct
    24661 agttttcaga tatttcagct gatttgtttg attttcactg ggatattagc tttacaaggg
    24721 cgcatccaac ccggcgatgt cgtgatgtat cagacgtatt tcacgacgat cgtcaattcc
    24781 gtttctggaa tgatcacgct gattcctacg atttcaaaag gcatggaatc tatcacttct
    24841 attggcgaac tgttgattgc tggcgatgta gaaacttatc acggtaagaa aaaaatccaa
    24901 catttggatg gaaattttcg ttttgaggat gtttcttttc tttatccgga ctcactcaaa
    24961 ccgattatcg atcatttttc tctagaggtg aaagctggag aaacaattgc ttttgttggg
    25021 ccatctggct ctggaaaatc tacactcctt aacttattga ttggatttat ccagccgact
    25081 tcaggaaaaa tttttctaga tgatcagcct ttggatgaac tggatatgcg aagtgtgcga
    25141 aattatttag ccgttgttcc tcaaaccact ttgttgttct ccgcttcgat caaagagaac
    25201 atcacttatg gcttgaaaaa cgtatcaaaa gaacgtttag atgaagtcat cgaagctgcg
    25261 caactttctt cactgattga ccagcttcct gatggtctgg ataccttagt aggcgaacat
    25321 ggcaacaagc tgtctggtgg ccaaaagcaa cgtatctcca ttgcacgagc gttgatacgt
    25381 cagccaaaaa tcattttgct agatgaagca acctcagctt tagataacca gtcagagaaa
    25441 aaaatccagc aagccctgaa ttacctgaca gaaacaccca ctacttttat tgtggctcat
    25501 cggctttcaa cgatcaaaga ggcagataaa atcgttgtga tcgatgaagg aaaaatcgtt
    25561 gaaataggta cttacgaaaa actgttagct caaaaaggct atttctaccg cttgaaaaat
    25621 gaatgacaaa aaataggagc tatgacaaga cttttgtcgt agctcctatt tctgaataaa
    25681 cggtgttctc ctgccaactc ctcggtcaca agtcacaata gtcatcaaac atgagaagct
    25741 gttttttgaa attgttcctt gtaactcaga gttaaacggt aggctcacga cctcttggta
    25801 aatggtgttc aagagacagc ctatcggcaa taagaagcaa ttttgtcatc gctcaaggcc
    25861 gaacgtctct ttcacaccct ctgttgtttc ataaaatagt tttattcgct gtaaaggtcg
    25921 cgaactggtg tcatgatgat acggttcaag tcattgatga ttgtactgaa gccttgctct
    25981 ttttgcatca agtcattgat tacttcttct gattggattt tcacagccat ttcttgtgct
    26041 ttcattgcat cttcgtcgct gaactcttgt ccactcattt gtttttcttg taattcgatt
    26101 tgcatcgctt ggaagtcttt gaacagtttg tgtgcttctt cgtttgcttg tactttctcg
    26161 tacgcttctt tcaacgcttt gaattctggt aattcacgga tctcacgttc gatttgattc
    26221 gcgctatcgt agatgtttga catgttatgc cctcttttct tttagtagtt actctctatt
    26281 gtaacatggg gttattcgtt ttcaaagggg ctactctcct tgatatttcc gccagaggtc
    26341 tttagccgct tcaccaattc cctgaacgcc ttctttcacc atatcgccga agtttttcag
    26401 cccttctttt gcttgttcac catattgatt caagttatct tgccaatttc cagactctcc
    26461 attagaattt cctgtgtttc caacttgatc tgcagctacc acttgtcctc ctgtggcata
    26521 ggcatctgct acactaaatc ctgtttttga agtatacgga aggatgcctt ctgccgctga
    26581 tttgaagact ttcccgacaa catttccact tgtcccttcc aaatagtgca gcttgctgga
    26641 ttcttcgtaa cccatccatg tcgaaatcac tacgtctggc gtgtaaccga tcatccattg
    26701 gtcatttgct tttgtggcat cgaaattagt ttctgtggct cctgttttcc ctgctatcgt
    26761 ataacctgca ggattcgcag cagccgctgt tccatttgaa aatgtaccaa gaagcatgct
    26821 ggtcatttca tcggctgttt cctttgatat gacctgtttt ggttttttgt ttgtgttatc
    26881 gacgatcacc gctccagtcg aatcaacgat tttcgtgata agatgcggcg tatacatctg
    26941 accatcattg gcaaatgcac tgtaagcacc cgccatgatc aatggagata ctcctttttc
    27001 cagtccacca agggctaagc cataatactt atcagatttt gctaaaggca ggccgaattc
    27061 ttctgcttta tcatatcctt tttgtaaacc gatctcatgc aaaagccata cagtaggcaa
    27121 attcaaactt tgtgcaacag cttgatacat cggtacttct ccttggtatg tcccgtcaaa
    27181 attatgcgca tcgtaataac tttgcggttc gtctttcaag atactggaag gcttgtatcc
    27241 agcttccagt gctggtgtat atacgcttaa tggtttgatc gttgatcctg gtgaacgacg
    27301 catctgtgta gcaaagctga atcctctaaa gacatgatct cctcttcgtc ctactagtgc
    27361 ttgtacacca cctgtattcg gatctaaagc tactgaagca gattggacca tggcaccatc
    27421 tgaagcattt gctgggaata atgcactgtt ttgataagta gcttctaatt gcttttgata
    27481 atcctgatct aaagctgtat agattttata gcctttattc atgacatctt cttcatcaag
    27541 accatactcg ttaactgctt catcgatcac tgcatcaaaa taataaggat aacgataacc
    27601 agaatctgaa ttatcgtatg tgtcattaag atacgcgctg atatctgtct ggctttctgc
    27661 ttgttcttgt tcttttgtga tttttccgtt ttcttccatt acacttagta ctgtgttttt
    27721 ccgatcattc gcattttttg ggtgatcgat cggattataa ataccaggac ctttcagcat
    27781 tcctgccaaa gtagcagcct cagacagcgt aacattcgtt gcatctactc caaaatactt
    27841 tctggaagcg tcttggacac cccatacccc gttgccaaaa taggcattgt taagatacat
    27901 ggtcaagatt tcttctttgc tgtacttctt ttcgatctcg attgccaaaa ataattcctt
    27961 cgcttttcga tcaaacgttt ggtctaatgt cagatacgca tttttggcaa gctgctgagt
    28021 gatcgtactt cctccgcccc ctgatgttcc gccagacaag accattcgga ctgctgcacg
    28081 ggcaatccct ttgatatcaa acccgtgatg ctcgtaaaaa ttcctatctt ctgtcgagat
    28141 tactgcatct tggatatagg gagaaatcgc gtttaattct acatatgttc ctttttgggc
    28201 gtacagtgtg ccagcttctt cgtttttctt atcataaatg actgtcgatt ggcttaatcc
    28261 tgattttaac gtttcgacat ttgcttgttt tgctaaaata aagaggtaga tacttgtcac
    28321 taagacacag atcaaaccaa tcagcaaaat caatttgttc acatgatatt ttttccagat
    28381 gcgctttcgc catcgatgga attgtccgag atatggtctg atccaacgcc aaaaactgat
    28441 caaagcagct tttatctttt ctaaaatgct ttgaaaatcc atttgcttct tctcctgtct
    28501 atttgattca atcgtcttat tttaccatag tgaaagagtg ttttcttcct gcttaagttg
    28561 tagttcaata tgttttttta cgttagtgca agtagagtaa cataagaatg aacagaaaaa
    28621 tgtaagtttt ttcttaaata tgttttaaaa atggtacaat tagtctgcga ttgcactgaa
    28681 aggagtacac ttgtgaaaat cacgatcact ttacctatga accatcctga aacaacaatt
    28741 cgcgacttgc ttgaaaagga atggttagtt cctagaaagg ttcgacactt tttgcgtata
    28801 cgtaaaaacg tttggatcaa tcaacaacct gctttgttcc atcatgaagt aaagggcaat
    28861 gaccagatca cattgttatt cgaagaaaca gattataatt atcaagaagt tcaattagga
    28921 gatgcgaagc acatccgtgt tttatatgaa gatgagcatt tgattatagt ggataagcct
    28981 gcaggaatga agactcatcc aaatgaaccg acagaaaaca gtaccttact caatcattta
    29041 gctgcttatc tcgacactaa acagcaacga ccttacgttg tccaccgctt ggacaaagaa
    29101 acaagcggtg ccgttttatt tgcaaaaaat ccatttgtct tgccgatact tggaagaatg
    29161 ttggaacaaa aaatgatcta tcggagatat caagccatcg tatggggaga aattgccaaa
    29221 gatatgacca tcaccaataa aattggacga gatcgccatg atcggagaaa aagggtcgta
    29281 gatgataaaa atggtcagag cgccttgaca catgtcactg tcaacaaagt actttctgaa
    29341 caaacacaag tctattgtgt tttggaaacc ggacgtacac accagatcag agttcattta
    29401 gctcataagg gacatccgat cgttggagac cctctctacc aaaatagaac agctgaacga
    29461 ctgatgctcc atgcattaga actgcacatg atccatccat ttactcaaga aaaaatagtc
    29521 ggtaaagctt tacctggatt atgggaatag aaataaaaaa gaacagagtc ttatcagcag
    29581 ataatgataa gactctgttc tttttgtgta atttgtctgc ttacttttga tgttcgtgtt
    29641 ctgctaacag caaactaccg ataatcccac tttcattcgg cagtccccaa cgaacgatat
    29701 attcacttac ttgaggtgtt tccatataac cacccatcaa ttcaacaaat tgctgacgga
    29761 ttttttgaag gagatgatcc tgattcatta ccccaccgcc taaaataatc ttttcaggtg
    29821 ctaacgtcaa cgtatagttg atcagtgctt gagcaatata ataagcctga atagcccaaa
    29881 caggatgatc ttgcggcaga tcttgccctt tgatccctgt gcgtgcctca atagacggtc
    29941 ctgctgctaa tccttctaaa caatctttat gataaggaca agtaccttca taatggtcat
    30001 ctggatggcg tttcacccaa atatgtccca tttcaggatg tgcgatccct gaaaaaattt
    30061 cttcattgat cacgacacct gcaccgattc ctgttcccac ggtaaggtaa atacaactat
    30121 tttttccttg tgctgctcct tgcttcaatt ccccatatgc agcagcattg acatccgttg
    30181 tccaagcagc aggaatattg tagcgttcct tgattgtgcc aagaaaatcg aattgtgccc
    30241 accctttttt gggagttgcc aaaacgtatc cgtaattatc ttttgttttg tcgataccaa
    30301 tcgggccgaa tgagccaatc ccgattccct tcaattcata acgatcaaaa aattcaaata
    30361 cctgatccaa tgtttcttct ggtgttgtgg tagggatact taatttttct aaaatttctc
    30421 caccatctga aaccgcacac acaaattttg ttccgcctgc ttctattccg ccatacattc
    30481 gttttcctcc tcgaatccga ttgtccgaat ctcataaggt tggattattg ggacaaccgc
    30541 ttgcttcgtt ggttcttcaa gcagattcaa tatgacagtt tttccattgt ctttagttat
    30601 ttccaatttt tctaaatgac tagacatatt gaaacctctt actactactt tatcactaaa
    30661 cttgctgcgt ttcaaagctg taattgcaaa cgtttcactt tttatcgtta aatattgttg
    30721 ttttgaaatc aatgttcctt catgatgttt gatttgttga gtggaaaaag gaatctgtgc
    30781 tgcgtatgca tgcaaataag tagagaattt ttcttctggt ccatgtagtt caatgctgta
    30841 attgaaccga tgctcaccta gacactgtgc ttctggtgtt ggaaaatatc cccaatcgcc
    30901 tagttctccc acactgcgaa gtagcgtcac tgcgatttgt ccgtcttctg ttacttcata
    30961 ttcattcaaa ccaaagtttc cgactgttac accgtactct tcatcatgaa tattgacaaa
    31021 tgcttgctga tgttgcggat tcgtggggtt ttcccaactt ttagacacat ggtttgggcg
    31081 cttcaccact tcaaaaatac tatctgcttc gtgatgttcc acatgtaact ttgtaggaaa
    31141 caagacacgt aagcgatgat ctttcatctg attgtcgaga gtcgtttcaa agtcgatttt
    31201 tttactatct tttctgattg tcatgattgt ttttatttga aggattcgtt tttctttgga
    31261 tcgttctgct tttcgataac gaaattccac aaccatctgc tgttcttttt ctaaacgctc
    31321 gtctgcagaa acagggattt ccatttcctg tagaatactt atttttgcta tttccggtgt
    31381 atctactaat acttttactt cagagttttc tttgttactg gataggattg gctgatcaca
    31441 gaaaggttgt ttaaagatat actcatttcc tatatcgccg atatcttcaa aaatatgcag
    31501 attattcaac tttcgtttgt ttttgcgatc ttccattgta atggtaccat ttttttcaat
    31561 cgtcaaatgt agagggccgt tttcgatgat tcgtccactc tgatgaatca ttgtttcctt
    31621 ttcttctgct ttcgcttccc cttctacaag cgcgaatgtt tcccaagaaa atgctggcat
    31681 gttttctaat ggtaaagtca ctttgatcat tcttgccata tatggcacac ggaaacggtc
    31741 tttcggcaaa tcataaccaa attggacagt ttcttcagat aaaatagctg gtatagtggc
    31801 cccacttttt gtttctacat gatacttacg tttaggcaaa ttttctaact cttgatatag
    31861 ttgttcaggt attccttcag cgaatctttt tctttctaat tcgatggtta tttctgcttc
    31921 accagtttta tccattcctg cagtatttac aatgacaaat ggaaagctgt cttttgggaa
    31981 tccggtcgta tcgattgctg cggtcagctg ttctaaggaa tcttgtgcaa gatactttcc
    32041 aacttcatct gctttttcaa aacgaggtat catttcccga tggacagaat cgactgaaca
    32101 accgcaaata ctgtcgtgag gatgattttg cattaatgtc ttccaagcat aatctaattg
    32161 atcatgggga tagtttccac taacttcata agccatcgtc gcaagtggtt ctgtaatatt
    32221 ttctagctgt cgctggacct tggtattcca ttgttttaag taaacccgag cagatgctgt
    32281 attggctaaa gtataccagc catccgtttc ttgacttgtc aattctcctt ctactgagcc
    32341 taagtcttct ggcacatctt tttgaactgc ttccagatag tcagtaaaat tagaatgaat
    32401 aaactcgtaa tctggaaaca gttcatttgc gagatggatt gcttttgata tatctttttg
    32461 taccggttga tgatcaacac cattcatcat caataagtgg ttcgtagagg catattgttc
    32521 tgcatccgct aatttttgct tccaaaaggc aagagctgct tctttttcta ctggaatctc
    32581 attcccgttg ctataccaat tggcaaatag cagaccaaag attgctgttt gatcaggtcc
    32641 tttccaccac atctctgagt attgggaaga atagttttct gcttccaaaa cttgattatc
    32701 aaagccaatt ggttttacac cacggccaaa cgctgctgct gaaataccag cttgtttcat
    32761 taattgaggc gtctgtccca tatttccaaa tgtatcggga aaatagccta acatgacagg
    32821 ggttccccat ttacggcttt cttccatgcc aatcagcata tttctcacgt tcgattccga
    32881 gctgataaga aaatcatctt gcaagatata aaatggtcca attctcaatt ttccttcatt
    32941 gattgcttgt tggattgcct gtcttttttc tggccttact tgaagatagt catctaaaat
    33001 aatcgtttgc ccatccaaat gaaaactgtt gaaggacgga tcaacttcaa atagctctag
    33061 tagatcgtct atcaattcaa tcagtcgcat gtgatgctgt tcgtaagcca tgtaccattc
    33121 acgatcccag tggctatgag aaatgatata tactttcttt ttcatttacg atacctccta
    33181 cttttctact ctaatatcaa agtaatccat gactaattcg caaaacatca tatttgccca
    33241 agaaaaccat tctctcgtat aattttcagg attgttcaca tcgaatcctt cgtgcataag
    33301 gtgtgttcct gcgtcagttg ctacaagtaa attcaatatc cgctcttttt cagatttgtc
    33361 tgacgttgtc attccttcca tagctaatgc aatcggccaa acgtattttt ctggcgtatg
    33421 ggaggatcca attccttttg cgtactttcc ttcatagaaa tatggatttt ctttactcaa
    33481 caatgttctt cttgtttgca gataatcttc ctcttgttca gaacaatatc ccaagtaagg
    33541 tgcggcgatc agattgggca cattactgtc atccatgact gtggcgtttc ccattccatc
    33601 tacttcatat gcataaatcg tttgcccttt ttgattaacc gttcgtccgt gattctgaat
    33661 cccctcttgg atctctttct tcaatttctg tgtttgctgt acaatttctt tatcatcgag
    33721 tatcgtttga aaaatttctt ccacataacc taacacgacc accgcaaaca tattggatgg
    33781 taccagatat ccataacggc aagtatcatc gctaggacgg aaaccagacc atgtcattcc
    33841 tgtaaaagct gtttttgcac ctcgcccgtc attgatcagc gtatcttctt ttcttgtcgt
    33901 gtctcgtaca aatgtataag gagagtgttc atgatcttgc tctgttttga acacgtccaa
    33961 gatttttttg ataccttcaa taaaagattg attgaattga tcagtttttc ctgtattttt
    34021 gtaaagaaga taggccaact gtattgggta gcataaagaa tcaatttcat attttctttc
    34081 ccagatccaa tcgttcattt ttgtatgatc tgtttggtgg ccagcaccgt tagcttcttc
    34141 gttgaaggca ttcgcatatg gatcgatatt gatataaaaa aattgctttt tgactaaccc
    34201 gctgatcatg tcagctaatt cctcatcttc ttttgcaatc actaaataag gacgaacttg
    34261 tgcagtagag tctcttaacc acatcgctgg aatgtctccc gtcaataaaa aggtggttcc
    34321 gtcattttgc tttttgactg ttgtcaacag agtattggca aatgctgctg taaaattctt
    34381 tgcccagtcc gcatggatat ccccacattt ttgtgtgata tcttccataa atttctgcac
    34441 cgattttggt atatctttat aagccactaa taaaaccttc tttctatttg atatatcttt
    34501 aaagattata cttcatacag ccaattatgt ctacgctttc aaaaaatatc tttttattta
    34561 ctttttcatc cattatggta ttatttgaat atgatatatc attaagaggt gtttatatga
    34621 atcaaccatt gtaccagaca atatttaatg atttgcaaac ggctatttta actggtgaat
    34681 tacctgtaga cagccaacta ccaactgaaa aagaactttc acgtacctat aaagtaagca
    34741 gaatcacttc caaacgtgca ttgactgaat tagagcaatt aggacttatt tatcgcgttc
    34801 gcgggaaagg cagtttcgtt aaatccgctt ctccggaaca gattcttgct tctcctacaa
    34861 aaacacgacg gattctgttt cttcttccat atgtcgctga tttgtctgtt ggtgatttta
    34921 gcaagggatt gaaccctgtg atgcaagata aacagattga tgtcatgatg acgacgcttg
    34981 actttctaca aaataaaaca gcaaaagagc taatcaatga atttgacgga ttgatttatt
    35041 atgctcttga tacagaacaa catcttgact tactttttga gctttcattg aaagaattcc
    35101 ctgtcgtcat attagataaa aaaatctatg agttaccttt tcctaccgtt ctttccgata
    35161 atttccaagg cggtgcgctt gctgcctctt atttgatcga acagggacat tcaaaaatcg
    35221 cttatttgtt tggtacgcag actcatccac aatccgttcg acagcgttac cttggttacc
    35281 ttgaagcatt ggacaaagct ggtttgactt tccacacttc actggatgac aaaagcgcga
    35341 cattagatga attgctttct tatatcacta agtatgaagt aactgctttt gtttgtgaaa
    35401 acgatgttgt cgctatccag accatgaatc tcttgagaca gcatggttat catatccctg
    35461 aagatttttc aattatcgga tttgatgata tccaagcagc agctcttgtt gacccgccac
    35521 tgacaaccat cgcacaagat tttgaacagt taggtaagct tgcgggagat gctttggtca
    35581 cttggttaga gacaaaagat cgaccatccg atagtaaatt tccagtttca ttcatcaaaa
    35641 gacaatcaac aaaggagaaa taaaatgaat atcggtcaaa tagatactag acatgcaaca
    35701 gcgaatcagc caaatttctc taatgggaac tgccagcctt atactggcgt tccttttggg
    35761 atgaattatt ttgcaccgca aactacggat caaaacggca gctggtggtt tcatcctgat
    35821 gaccgcgttt ttcaaggcta ccgtcttacc catcaaccaa gtccttggat gggtgatttc
    35881 agtcatatgc tgtttacgcc gatcaatgga aaactacagg agaacaccct tttccatgct
    35941 caaagttctt accgcccaga agaaagtata ttttgcccga ctcatctgtc gatctgtcaa
    36001 ttacgttatg gcatctgttc tacattgata cctagcatgt atggcggaat cttatctatt
    36061 gattacagca aaaatgacag cggtttactt ttatcctttc cgggacgcca tcagctcttt
    36121 gttatagatc cttacacagt ggaaggaaaa atcacacatt ttgctggttc agaagatcct
    36181 gatttttctt tttattttat attgagattt gaatatcctt taacaattga tgagttagct
    36241 gtttccaaca aaaatagcga ctcgatcccg ttgtattttg aacagacaaa aaaacaaact
    36301 atccgatttg ggacatcttt tatcagctcg gaacaagcag cttataacct ggaactagaa
    36361 caaaaccaaa gcaaagagac ttaccttgct cacagtcaag aacaatggca gtcctatttt
    36421 gatcgtatac agctcacaca ccatgatcaa caacaattgc ggacgtttta tcataattta
    36481 tatcgggtgt ttttatttcc acaaacattt tatgaaattg atcctagtgg gcaaaaaatc
    36541 cactatgata cgactagtcg atctataaaa cctggtgttc tctatacaaa taatgggttt
    36601 tgggatacgt ataaaacggt ttatcctctt tattctttga ttgctgtaga aaaatacgaa
    36661 gaaatgctgg aaggtttttt gaacagctat agagaaagtg gctatttgcc aaaatggctg
    36721 tctcctgatg aacgtggact tatgcctggg acattgatcg atgcggtcat tgcagatgct
    36781 gctgtaaaag atatccgacg agacttgatg cctgaatttc taacggcaat ggaaaaaggt
    36841 gctacggttc aaagcaaaca tccaaattat ggacgccaag gaacagaaga ttacttgaca
    36901 tatggctatg ttcctaatca ctaccacgaa tcagtcaatc acacattaga ctatgcttat
    36961 agcgattttt gtattagcca agttgccaag gtactagaag aagaggaaat gacgaagaaa
    37021 tatcagcagc aatcattgaa ctatcaaaat atctttgact atgagtcagg atttatgcgt
    37081 tcaaaagata aagatggcaa gttcagagaa ccattttcag ctaatcgttg gggacaagat
    37141 tatgcagaag gaagtgcttg gcaaagcagc tttgctgttt atcacgattt ttcaggactg
    37201 atcggaaaat atggtggcag tgagagatat aaagaaaaac tcattgagct gtgcaatcag
    37261 cccccaacgt ttgacccagg aggatatggt tttgagatcc acgaaatgag tgagatggcc
    37321 gcgatagaat ttggacaatt agctatttca aaccagccaa gttttcacta tccttacttg
    37381 ttcagttata taggacaacc tgaaatggca cagccactgc ttaaacagtt gatgactcag
    37441 accttcgacg attctccaat gggttaccct ggagatgagg acaatggaag tatggccgct
    37501 tggtatatct tcaatagtct gggattttat cctgtcacgc ctggaaccgg tgaatatgtt
    37561 atcggtatgc cattaattga caaggctgtt ttgcttttgt caaatggaaa ggaattgacg
    37621 attgaagcaa cgcctaatcg gccgcagcaa caatttatcc ataaaatcaa acggatggac
    37681 aaagaatata cgaaattata ttttacccat caagatttgc tgaaaggcgg cacgatctct
    37741 taccaattag ggatcatccc tcaccctaag aaatatcaag caaaagattt gcctttttcc
    37801 ttatcaaact ataattaaca agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa
    37861 cagccaagca aaaatagatc ctcatatttt tcgtttggtt ggtcttatta ccgcagatgc
    37921 aagcttttga acaccgttta ttgagaggtt gtgaaatcag cgttctgacc tgagtattag
    37981 agagacaaat ggcaaaatag ttcctcatat tttgccattt tttgagctaa taccgagggt
    38041 cagctgattg aacaccgttt agtggagagg tcgtgaaaaa gctgtttagt ttcgagtatt
    38101 aagaaaaatt ttgtgaaaat ggctttccac atttttgcat aaatttggct taataccgag
    38161 aaactggctt ttgaactccg tttatcgaga ggtcatgaaa aagctgcctt gcctcaagta
    38221 ataagaacag cccaaaatta gatttctaga caatttttta taagttattt cattacattg
    38281 gctttttctt ttatgattct ccactgtttt ctcggtactc tttaggggat ctgccgacga
    38341 ttttcttgaa taatttagag aaatacgtgg tgttgttgta gccaatctca cttgctatct
    38401 cattgatgtt ttgattactg tgcaaaagca gttgtttcgc tttttccata cgtaaatggt
    38461 tcaaataagc tgaaaaactt tttttcgttt cttttttgaa cagctgtcct agatacatga
    38521 catttaaatg caggttttct gacacttctt tgagtgtaag tggttcttga taatgctggt
    38581 gcaggattac gattactttt tccacattgt cactgtactt tttctgtctc tgatgctttt
    38641 tgatgaatga cagaagtagc tgctgaagtt cttgcaccgt ttttgcctga tggatttttt
    38701 cgattccttg taaatattcc tcatcttcca gatcaatcaa ttcccgttga atctccataa
    38761 acagtaaaaa actggaatat cgtatatctt ccggcatcat ggctgctaac tgaaactgtt
    38821 caaaaaaatc agtgatcatt ttttgagcga cttccaattg tttattttgc agcacgcgtc
    38881 tgaaacttga aaaatcgatg gtttgatctt ttgtttctgc ttgtactgcg taaaaaacat
    38941 gctcgtcttt atctccataa aattgatgca actgtagatt gtctttcgct tgttggaatg
    39001 tttcagggat tttgtccaaa gaatctgtct cttccccgat actgatcagc cattcctgat
    39061 caggaaagtt ttcttgaaca aaacaacaga agccatcaac ttcatctgcc tccaacaaca
    39121 acgtgatcaa tacacggtct ccgtaatttc gttgatagta aaagatttgt tgccgttctt
    39181 gcaaccatct attgataaga gtatcatgag ttcttgccag ttgaatcaat actatcctat
    39241 gctgcttctc acttatttgt cctattattt cttcttcact tatttcatcc aattcttcat
    39301 tcaaccattg actgaataac agttcttgat agatttcttg ttgatttttt tgttcatttt
    39361 gctgatctaa ccgtgttttg attttcttga ggctttcaac caattcgaac ttattcacgg
    39421 gtttcatcaa ataattcacg gcgcctaatt gcatacctcc tttcaaataa tcgaattctt
    39481 ggtatcctga taaaatcata aattcaaaat tatgacgttc tttttgagcc gcttcaataa
    39541 attccagtcc atttatttct ggcattgtca catccgttac tactaaatca atctgtcgct
    39601 gttcaagtac agctagtcct tctttggcat tctctgctgt tgctactatt tgaaagccta
    39661 aactttgcca atcaattatc ttttttaacc cattcaaaat catgtattca tcatctacca
    39721 ataacacttg atacacgctc atccctcctt gaatgtaata gaaacggtca gtccacctgt
    39781 ctcattattc tcaagcgaca tataataact gctgccgaag ctggcacgta aacgttcatt
    39841 cacgttttgc agaccgatag aaccgtgaag ttccacttta ggatgagaca gtttttcttc
    39901 aatctgtttt agccgttttt cagtcatccc ttttccatta tctttgatga tgatccttac
    39961 ccgtttgcct tcctgcaaga ctttgacact tagcgcatta tcgaatcgag taaaatcaat
    40021 accgtgttta aaataatttt caactaatgg ctggatcaca aatttaggta cttcgatctt
    40081 ttccaaatca ggatcgatca taaaatgata ggctactcga ttaggataac gcatttgata
    40141 caaatagaca tatttttcgc aaaagtccag ttcttctttt aaagtgatcg ttttctcttg
    40201 attcgtattg tttcgaagca aagcggaaaa cgcatacaca acatctgcta attcttcact
    40261 tccttcgctg attgcataca ttcggatata ttctaatgta ttatataaaa agtgtggatt
    40321 gatctgtgct tgaagcgccc gcatatgtgc gtcttgctgt ttgatttcta atttgtagat
    40381 gtcttctaca aactgattga tgctatccaa catttcgttg ataccgattg aaagttcctt
    40441 cagttcatat tccgtgtttg ttatgtcgat ccgcgtctct aaattccctg ctgctgtttg
    40501 atccatggcc tgcatgacga tagatacttg ctgtgaatat cgtttgaatg ttttataaag
    40561 aaagataagc agtatattgt ctaaaataat gataccaatg atcagcgggg caagattttt
    40621 tcccgtttcg atttttacac tttttttatc tagtacagct aatatacgat aatcatcaga
    40681 cagttcttgg tgttgaacaa aattgttttt ttccagatcg tcaatcggca attgtgaatt
    40741 tttcttcaga ctctctgtga tgatcatttc ttgtctttca aacgtttctt tagctatttt
    40801 attatcatgg aatgtgtata gtcgatttgt cgttccagaa atcatgtata aagagagtcc
    40861 attgaatgac gttagatttt ctaaaatcat atctagttct ttcttttgaa aaccaataaa
    40921 aatacttcct gactcagctc ctccttgtgt gataggcaac ttgatataaa aggcgtctag
    40981 taattttggt gtcccctttt ttatttcccc acctttgttt tctctagaag attcataata
    41041 ctccggtagt tgattcaaca cgatatatat cgcacttagt tcttcataat ttgcgtagag
    41101 atctttgatt tgatttggga aagaaactaa ttcatcggta ctgttttgct gttcgtatac
    41161 atacatcaga tactgatcta tgggttttgt taagtaggtc gtcacatttt cgatcttttc
    41221 ggaattccct acaagctggt ttttgatgat cctactcaag gttgttttat cattcagcat
    41281 tctgcttgtg ctttgaacgg catcacttgt catgttttct gcctgttttt gattttgttc
    41341 ccaagtatgg atactaaaaa taacggtagc agtcatcgtt acaccgatca tgataaccgc
    41401 gtatttccgc aacagacgat ttagtaaggt tccttttgaa aatagaatcc gaaacttatt
    41461 cataactttc caactgttta tctaattctt cacgccaatg tgttgtcatc gtaccattcc
    41521 aaaccgtcaa aagaccaatg acaccaaaca ggatcaatcc tttgaattgc caagtcacgc
    41581 ctaaaattat accgctacca ataattattt ttagaaaaga cgaaaagctg gaaaaaaccg
    41641 aaataaaact tagctttagc agattaggaa cagagatcgt atattcacta tctaaaatca
    41701 tactgtattg ataagtagca aataggcaga gcaaggcaaa aagtaaaata aaatcaatag
    41761 ctaaaaaaat gagccctttg atttgaacag ataaatataa attgtaactc aataaagcag
    41821 taccagataa aaataaacta aatagcagat tgcctcttaa aaaatttctt ttatatatat
    41881 tccagcctct ttttattgtg atttctttat actcgaatcc gtacaaataa aatagttcat
    41941 tgactgtttt ccatgccgca cctaatccaa agatgatccc gccggcaaat gtatatatcc
    42001 agaaatacaa ggttagtttt ataatgaccc agatccggat aaataactct tccaaaacct
    42061 tccctaacat atcttctcct ccaaacatta agtttatact ttctcattat acaaaaaaag
    42121 gttgggcaat cagcaccttg acctgattat tagagaaaca aacggaaaaa tagttcctca
    42181 tattttgtcg ttttgtgagc gaataccgag ggttagctga ttgaacaccg tttaccaaga
    42241 gtttgtgaaa tagccgctta gtttcgagta gtaagaaaaa ttttgtgaat atggctttcc
    42301 acatttttac atatatttag cttaataccg agaaactggc ttttgaacac cgtttattcg
    42361 gaacaagagg ttgtgacacg tcttttgtca caaccccttt gtctttattc attattattc
    42421 ttgtcggaac tcatcatatt gtttttgcat ttctttcaat accttatcat agccagcctt
    42481 gtccaaagcg tctttcaatt tcggtaatgt ttcatctgga tcaacagtcc ctgtattcaa
    42541 tccatctaaa tattgattca tgacattaga aatattactt aattctgttt tgacactgtc
    42601 agtgttgaag ctgaatccta aaatcggtga agtttctgca tcagcaatcg attgatcacg
    42661 tttggcaatc atgtcatccg taatagactc ttgtgtatat agaatcttgt tgtttcctgt
    42721 attccaagca gacatatgtg tatttggctg ataaccatct agcaatttga ttttatcatc
    42781 tgaaccatcg actttttccc atgcttctcc ctcgatcccc caaacaagcc cattcaataa
    42841 ttctggatca ctgttaagca gccctagaag ttctactgat ttttctttat tttttgaagt
    42901 atttgatacg acaaaatttg ccatttgtgc ttgagctgta gattttagtg gaacagtaat
    42961 tgcttttgag acaagttctt gttttgctgc atttgtcaaa attgtatcgc cataatcata
    43021 cggtccttgt gtttctccac gcattagcca agtatttcct tctagtggat aatccttatt
    43081 gctcgttgca gcgtcacttg gaatgagtcc ttgtttgtac cagctatgca ttgtacgtaa
    43141 cagtccgata tacgtttcat tatcgtattg gttgatgatt ttcgaatcat ctccatccag
    43201 atcaatagca aatggtaacg tattccctaa aggataatca aagttccctt ggactttgaa
    43261 gccttgtcca atggcaaaag ctgccgtgtt tggttctttt tcatggaatt ccttcaagac
    43321 tttttctgca tcagcgtaag attggattcc atcaattgaa agatcgtatt tatctagtaa
    43381 agctttgtta aaagtaagta cttgttgagc aaagacattt ccattgactg ggaaagcata
    43441 taattttcca ttgaccagat ttcctttaat atatgcttca tctaaatcat tataagcttt
    43501 ttcagcgtac ttaggcgcta attcagtcaa atcagcaaag gctccttttt gggcattgga
    43561 aacatagttg ttcgcaaagg cgatatcata attttccccg gaagaaatga tgacgttcat
    43621 tttcttttcg taatcgcccc agccaatgta attgatattg acttttacac cgatctcttt
    43681 ttcgattcgt ttgtttgcta cttccatcaa ttgatcgaaa ttatctggtt tatctccgat
    43741 ttgatacatc tgcaaagtgt ccgaattgct agcttctgcc tggtctttag aaccagaacc
    43801 tccgcatgct gctaatgttc ccaacaacaa cgctgaaaca cctgtaaagg ctagtttctt
    43861 ccataacttc atttttcttc ctcctaattt tatgatttat atttttgttt ctttattctt
    43921 ttactccgcc gattgttaga cctttgacaa aatgcttctg gaaaaatgga tagctgatcg
    43981 caataggcaa cgtcgagatg actacgatcg ccattctggc agattctcct ggaactgagg
    44041 ctaatccgcc tgtcaattgg cttcctgctc ctgaattttg tgtcagatat tgaatattac
    44101 tttggatttt catcaaaaga tactgtagtg gaactagctt atcatcttgg atgtatagta
    44161 aagcattgaa ccagtcattc cagtaaccca atgcggcaaa taaactgatt gtcgcaatac
    44221 ctggaacagc taacggtaaa acgatctgaa caaatattcg cagttcgcta gctccatcga
    44281 tccgtgccga ttcaataatc gaatcagaga ccgaacgttt aaagaatgtg cgcatcacaa
    44341 taatattgaa gggactgaca gccattggca agatcaatgc ccaaatcgta tcttttaact
    44401 gaagaagatt tgtcataaca agataattgg ccaccatccc tggtgagaac aacatcgtaa
    44461 tcaaacaaaa aacggtaaaa aaacgacgga atggaaagtt tgaccgtgaa atcgcataag
    44521 catataaaga agtagccgtg ctattgatca gtgtacctaa aacagtaaca agtacagtga
    44581 cgaaaagcgc ttggaaaatt ttatcctgca tctggtcgaa caagtacgta taaccaaata
    44641 tactgaactt ttctggccaa aaactgtacc cgtgagtcga caatatggac tcatcggtaa
    44701 aagagatggc gataacaaat ataaatggca aaatacagga aatagcaaag agtgcaatca
    44761 ttatattaga gaaaaaatta gccgtcttat tgaaagagcg gacttggact ctctccactt
    44821 tctttttttt catttttttc ctccttagaa taatgcagaa tcgctatcaa aacgtcgggc
    44881 gatcgtattt gtgatcatca acaagacaaa gccgacaact gattggtaaa gtcctgcagc
    44941 agccgtcata ccaaaatctc ctgtggatgt caggccattg tagatatacg tatccaaaac
    45001 ttgagtcaca tcatataatg caccggaatt cctaggaatg ttataaaaca ggccaaaatc
    45061 agctcggaaa atgtttccta cggctaaaat cgttaaaatc gtcattagtg gaataagttg
    45121 tggtatcgtc acatgtttga tctgctgcca tttgctagct ccatcaacca tagcagcttc
    45181 ataataagtc ggatcgattc ccataacaga tgcaaagtaa ataatgctgt tataaccaat
    45241 ccctttccat gtaccaatga acaccaagat aaacggccaa tatttcggtt cattatacca
    45301 attgacagct tctcctccat gtccagtgat ccactgattg aatattcctt tgtcaggact
    45361 taaaaaagcg taaacaaagt aactgataat gacccaagat agaaaataag gtagtaagga
    45421 catcgtttga taaactttaa cgagtcgttt atttctcaac tcgctcataa tgattgcaaa
    45481 tacgactgag atcagcagat tcaaaaggat aaatgctaca ttatataaaa ttgtattttt
    45541 agtgatgaga aatgcttgat ccgaagaaaa taaaaatttg aaattttccc aacccaccca
    45601 aggactttcc ctcaagctag ccaagaaccc atctgctgaa atatgaaaat ttttgaatgc
    45661 tacgacattc gccattacag gtatgtaaaa gaaaaaaatc atccaaagca tacccggtac
    45721 agccatcagg acaagtgctt tataacgcca tagattcata aaaaaatgat tgaacccctt
    45781 tcgttttttc atctaaaaac ccctttcgat tcattcgcaa cgtaattata gcgctttcaa
    45841 taatgagaat aaataaacaa agtataggga aaatgaaaca aagtatagga gaggttgtga
    45901 aatcagcgtt ttggcctgag tattagagaa aaaaacggga aaatagttcc tcatattttg
    45961 ccgttttttg agctaatacc gagggtcagc tgattgaaca ccgtttatca gaggttgtga
    46021 attcagcgtt ttggcctgag tattagggag atcgtgtcct tactatctaa ctctgagtca
    46081 caaggaacaa tggggactgt aacaaaagtc ttgtcacagt cccttattcc gaacaaacgg
    46141 tgttccagaa gtagcttcta cggaaataag ccgaaatttc acaaaaaagc catccgaaag
    46201 tcagacggac aagtctgctt ttcagatggc gctacaacta ctctgcttat tcttttagct
    46261 atccattcaa ttatttttct ctcacttgtt ctagctcttc tttggtcatg ggtcgatatt
    46321 gccctttatt caaatcttta ggcagccaaa attttcccat acggattctt tttaaatata
    46381 agacttcttt tcctactgct ttgaccattc gcttaacttg gtgaaacttt ccttcatgca
    46441 aaatcagacg gataattgaa gtttctgtct cttgatctac ttgatcaatt tggagttttg
    46501 ccggcaatgt ctgctcatcg ccatcaataa tcagtccttc tgcaaataaa cggatatctt
    46561 cttctgtcat tataccgcta acctcagcta gatattcttt ctccacatgt tttttaggtg
    46621 atagtaactg atgggcaaac accccgtcat tcgttaaaat caagaggccc tctgtgtctt
    46681 tatctaatcg gccgactgga aaaagatctt cccgataatc ctggtcttcc aataaatcga
    46741 ttacggtttg atcatgatta tcctgagtag cagaaatgac tcctgcaggt ttatgcaaaa
    46801 tgtagtaaaa atctttttga tacgtaatga tttctcctaa ataagtaacc gcatcttcga
    46861 attcatttac ttgcgcttta tcgcttttca ccacttgtcc attgacttcg atcgttcctt
    46921 ttttgattaa tcctttgact tctttgcggc tgccaagtcc catatctgct aaaaacttat
    46981 ctaaacgcat gcgtttcccc catttcaaaa gagcatcaat ctagctcttc tgattcactt
    47041 attttatttt taatttatgt cgtaaacgcg ctattcctgg tcctagtagt ctttctgcca
    47101 agcggatttt caaagctaaa taaccataca ctaaagcccc tatacctgct actacaagaa
    47161 tcaacaatag agactggaat ttgctatctt cgtttaaaaa agcaccaaaa atcatctttg
    47221 tgatccacga agccaaaagc ataatcaagg taagaataaa gatcagcaat gtcctgcgcc
    47281 agacaaaact tggtttaaag cgggctactt catggattct ctttaagatc aaggtacaag
    47341 acacagcaaa tccgatcatt gtcgccaata aagggccgta tacttcaaat aaacgaatgg
    47401 ctgggtattg aagaaggagt ttcactagaa acccaataac caaataatta attgccgcct
    47461 tgttttcaaa cattccttgc agcatgttgg aaactagcat atatagtcct aggaagagtc
    47521 ctacaaaact tgcttggatc agtacattac ttcctagaga atccggtgta taaaataatg
    47581 tatttagcgg ataggcaagt aacataacac caaatgtcgc cggaaacata aaaaatgaaa
    47641 acaactgtaa atttccgctt gtcaattttg ccagacctac tcgatctttc aacgtaacag
    47701 cttctgtaat caacggtaac ccagtagacg cgatcgatgt tgccaatgca ataactacca
    47761 tcgtcaactt atctggattg gcactaaaaa tcgaaaaaag atccagtaac tgtgcattgg
    47821 aatactcggt ggatgcagac atgatcttca taaaagtgaa ctgatcgact aatttgaaga
    47881 tcgttacccc tgaacccaca ataataaacg gaatcgcttc tttaacggta tctaacaaca
    47941 attccttcgt tgcaatattt attttgtttt cgctgtagtg aataaacgcc gatgtatacg
    48001 cttgatgttt cttcaaaaaa tacaaaagga caagaatact tgctagcata ccgataaatg
    48061 ccgcgaaagt tgattgcgtg accgccgtca catagtcacc atccatgact ttcatgatga
    48121 tgaatgtagc caatagcata taaaaaatac gtgccacctg ttctacaatc tgcgacagcg
    48181 cataaggcat catttcttga ttgccttgca aataccctcg tatcacactc atacaaggaa
    48241 aaaccaatac agcgatactc aaagaacgca tcgttggaat cagttcttct ccgccaccag
    48301 aagcatgagc tagccaagga gaggcgatgt acatgaacaa agcaaacaac aggcctaacc
    48361 cgcccatcat ctgtaatgca cggataaata atcgtcgtga agtgccatat tcattcaatg
    48421 aattatagcg tgcagtttgt tttgcaatcg cagcaggtat tcctgctgta gagatcagta
    48481 gaaacaatgc atatatattg tatcccatat tgaataatcc attggcagcc ttagcatttt
    48541 cgcccatcca agcataccag ggaatgatat agacagcgcc tagcaatctt gaaatgatat
    48601 tactggcggt catccaagca gagcctcgtg ccattttttc ttgcggtgtt aattgatcta
    48661 atgctggttt tctttgatta tccatcggtc agattccaac tttctacaaa acattactat
    48721 cattttaatt ggtaaaacag aaactggcaa tcgattttat ataactttta taagaacaat
    48781 tgtttttttg aattcggttt cgtttttttg gtaaagtaaa gaaatataca gccttttatg
    48841 gctaacattt ataggagata tcaatgacac aagaaataaa aaaactaccc gttatatttt
    48901 taggtctttt ctttatcaca gttggattga attggttctt gctccctcac gatatagctt
    48961 ccgccggagt gggcgccatc ggacatttag ttgaaacggc cttttctatc caacgctcag
    49021 ttactgtttg gagcatcaat cttatgatgc tgcttctagc cgctgtccta ttaggaaaat
    49081 cagtattttt taagactgta gcgggaagtc tgctttttcc tgttgtttta gagattgttc
    49141 caaaagtgga aatgatctcc tcacatttct tttcactgat ttcaggaagt cttttgttca
    49201 gtcttggggt ttacacattg tacacagtcg gggcctcaaa tggcggtata acgataccgc
    49261 cgattatttt ccacaaattt tttcggcttc caatgccgca aggactttta cttaccaata
    49321 gtcttatcgt ctttttgaat tattatgttt ttggattgtt ggagacacta ttcgttctcc
    49381 tatctattac agtcagttca ttgtttatga aaatactcat ccgcttgtcc cctgtgtcaa
    49441 gaaaatttga ataaagctgg attttgtcat agcggaaaca aacgatatat taaaaagagt
    49501 gtgctataat aaaccaagaa attgaataaa gcgagggatc aatcaatgag cttctcttta
    49561 gaagacatcc gtcatttact attaaaagaa aatctattaa aagaatttgt ttcaacaaga
    49621 ggctggcatc ttgatgtacc agaaaaagct gaatttcttc agttaagtta tgattcaaga
    49681 aaagctgatg cttctacttt gtttttctgt aaaggaatga atttcaaaga agaatatttg
    49741 gattcagcaa tcgaacaggg tattcaatat tatgtatcag aacaaccata cgaaaattca
    49801 gcagtaggaa tcatcgtaac ggatatccgt aaagcaatgg cgcttcttgc tatggctttt
    49861 tacgactatc cgcaaaataa attgaaagtg atcggattca ctggaacaaa aggtaaaaca
    49921 actgctgcgt actttacaaa agcaatcttg gatcacacaa caaataaaaa aacagcattg
    49981 ctttctacta tgaatacaac gttagatggc aaaacatatt tcaaatccca tttgacaacg
    50041 cctgagtcat tagatttata tcgtatgatg tcagaagcag ttgaaaatgg tatgactcat
    50101 ctagtgatgg aagtttcttc acaagcttac aaaacacaac gcgtatacgg cttgacttta
    50161 gatgtcggaa tcttcttgaa tatctcgcct gaccatatca gtccgatcga acatccgaca
    50221 tttgatgact atttctattg caagcgacaa ttgatcttga attcaaaaac agttgtttta
    50281 aatcatgaaa gtgactattt tgatttactt agggaaacag ccgatctatt ccagattcca
    50341 acgatcactt atggtcgttc ggaagaaagc aattatcaag tgatccgttc agataaaggt
    50401 actcacggtt ttactttatc ttctcatgaa gatcgcttag ctatctgcga tactgcttat
    50461 gatattttgc tggctggagg attcaaccaa gaaaatgcag ccagtgcgat cattgccgca
    50521 gcactaacag gtgcctccag tcaagatgca caagacggat taaaagaagc tcgtgtacca
    50581 ggccgaatgg atcaattgat ccaatccaat ggagcgcatg tctatgtgga ctatgcacat
    50641 aattatctaa gcttgaaaac attgctgaaa tttgccaaaa atgaacatcc agatggccgt
    50701 gtaattgttg tattaggcag cccaggaaac aaagcaatct cccgtcgaca tgattttgga
    50761 aaagtgctat cagaaacagc tgacatcgcc tttttgacgg cagatgaccc agcatttgaa
    50821 gatccgaaaa aaatcgcgga agaaatcaat gaagcaatta ctaatccaga tttgatcgtt
    50881 cattatgaaa tggatcgtcc tgaagcgatt cgacgcgctt tagcagaaag cgctccgcaa
    50941 gattcagtcg ttattgcggg aaaaggcgtg gatccctatc aaaaaatcaa tggtgtagat
    51001 gaaccttatg aaggggatta tgcaatcgtg aagcggttga ttgaagaata atggtattta
    51061 tatactagtg gtttcccatt tctaagaaaa aaagggactg tgattttttg tcacagttcc
    51121 ttttttctta gaaatcattt taacttttca ctagctaatt ctgctgcctt ttctctttct
    51181 tcatttaata atttattatc atagaatttg ataaatggga accagataat gaatgcgatg
    51241 attgcacaaa tgactgctaa cacagcagct ttccaatcac caccactacc aacaaatgct
    51301 ccgatcccga ctggtgaagg ccacggcatc tgtgcaagca tcggtttgac gatttccagc
    51361 cgaatggcaa aataagcgag tgtagcagaa gccatcggag ctaagaaaaa cggaaccgct
    51421 aaatagggat tgtagacgat tggcatcccg aaaatgattg gttcattgat attgaataat
    51481 cctggtacaa gtgaggcttt tcctaacact ttcagctgat cagatttcgc gaggaatgca
    51541 ataaagatga tcaatcctaa agtcgcacct gatccaccaa cggttacata agaattgttg
    51601 aattctcctg ccaacggata atttgcgcct tgtgcgttag ctgccatatt agctaatacg
    51661 atcggggtca agaaagaagt gatgatattc gctccatgga tccctacgat ccacaatgca
    51721 tggatcaaga aataaatcac catgacacca agccatgtat tcgtcaactg cgtaacaaac
    51781 ccaaatggga tagcaatcat tttgaagata tccgttccta gtgcaactaa taatccattg
    51841 atgatcatca ccacaaacgc aatgacaaat gttgggatca gagcagtgaa agaacgagaa
    51901 actcctggcg gaacaacatc tggcattttt atgacccaat ttctcttgac acacatacaa
    51961 tacaattcga ccgcaataat cgacataata atagctgtaa agattcctga agttcctaaa
    52021 cgttcgacaa aagagcccat cctaatacca tttactacag tttctgtctc attgatagaa
    52081 gcaatcaagc tgattttacc atcagtcatg atcaattcag ggatacacat aaagaatgca
    52141 aaaacagata gtaatgcccc cgtgagcgga ttaacattta atttttgttc atctcgttca
    52201 attgacgtta attcataagc aaacacaatg gcaaagtaaa tagataaaat ccccatcgtc
    52261 attgtattga cgatcatgta caagtcacta actttgaaaa aagttgcatt aaaaaatcct
    52321 tctaaaaatg ggaacggtaa tggcaaaata tttaaaacca aaaacatcga gcctacaatc
    52381 gtgaaaggaa tagaagccat ccccgctgcc atgacagccc gaacgatttt aagttgcgca
    52441 attttcccca taggtcccat caggtatttc tgcaaaaaat caaacattag ttattctcct
    52501 ttcctaaata taacgctttt tcatattgtt taatacggat atactgttgg tcttggttga
    52561 gtgaaatttt ctttaaacga tactgtataa atgcactggc aaaaataccg cttacaacaa
    52621 gaatgaaaac aaataactgt cctccattgt tatttgccat aaaaggatac aagaatgtaa
    52681 atcctgaggt aaacaacact ggaatcacta ataaattaaa gacactctgg aacaaaaaat
    52741 accactttgt ccatggaacg ttcgtttggt gttttcgata aagctttact tgttctccta
    52801 ctgcaggcaa aatagctaaa atcagaatca tcggtaaact acttcctaaa cttttagctg
    52861 atagaagcag aatcatccat tgcatgttca tgaaaaagaa aaaggcagtc aggtaacgta
    52921 cgattagata acgattgaaa tacatgtttt tcaaactaag ttgctttttt tctaattcga
    52981 ttttcctttt tactgtatcc tgcacaatga tccttccttt ctatttattt ttttgataca
    53041 aataattcat ttcctgtgca acttccaata atgtcatcgt cgtcatcaga tgatcttgtg
    53101 catggaccat gataatttcc atttctattt ttgtcccgct ggcatattct ttcagaagtt
    53161 gagtttgtga ctgatgcgca tgaagaatct cttcatttgc ttgtcgcaac ttctcttcta
    53221 cttcttcaaa catctcttta cgcatcgagc gaaatgcttc atgtatcagt gtccgtgcat
    53281 ttccgctatg gaaaatgata tcaaaagaag tcacttgtag tgtttcactg tttatttttt
    53341 cagccatctg ttttcctcct tattcgatca gtttgataaa tttactgctg aaattctcaa
    53401 aaataggttc agctaagatt tcctgctgta cttttagctg gtcaactaac ctaacgattg
    53461 ctttagtcac gaccgtaatt ccttcatttt cgacataaga aggcgaaact aaaaaaacaa
    53521 aatgaatttc ttgatattga tcccactgca tcccagaagg aatcaatgca acggctacct
    53581 ttgtttcgat tccaactgga acagcgggat gtgggactgc cactgtatca ctgaatatga
    53641 tttgtcccat attttcacgt tgctggatct gctttcgcat ttctcttata tatccttctt
    53701 cttcatttat gctcagcttt tgcagcaaat catgaagaat ttcttccttt ttcggttcgt
    53761 acgtataagt cttgaaaaat ttttcagaca actgttcggt gtaaatctgc tttttttgct
    53821 gtagagaaaa tgctggtatc ggtctatctg aggaatgata gggtatagat tctccaacta
    53881 cctttcgtat tttacgaaca tcatcttcat tcaaaaaaat actgacatgt aaaactggaa
    53941 ttttgaaaaa cattgtcgaa agatcaatag aagaaataat caggtcgatt ccttttaaag
    54001 ttgtttcatt gatctcataa tagcctttta cacttaccac tgtaatattt ttaccgaatt
    54061 cactgactac gcgattcttt aatagttggg cactgccgta acccgttgca cagataatca
    54121 atgcatgtag tttatgcgca gctctttctt tttccaatgc cgccatgaga tgcaaagcca
    54181 aataggccca ttcatcttca ttgatggtat agtttttcaa agccggcatg ttgctgaaat
    54241 gcctttttac tgcgtcaaag actttgggat tttctttctt tatctcctca gtcaatgggt
    54301 tctcaatgcg aatacctcgg ctcaatcgaa tcatcatcgg tgacaaatga tcgagtagtc
    54361 cattgaaaag ctggagatcc tctactaatg aaaaatgaaa ttcctgagac aatcttttca
    54421 gaactttcgc taattccttt gatagttcct gatcattttg attttctttg tgattcgact
    54481 tagccatgag atgtaatgtc aaataagcaa tttcttcttc gggaaacgat acacctgcga
    54541 ttacctcaat acgttgaacg atccgctgcg ccacttgata ctctactcgc ttagaaactt
    54601 cttgttcgat gcctaattcc ttgatttcaa acccttctcg gagccgtttg atccccaaag
    54661 ccaagtgtaa agtcagattc tgtatgataa aatccgaaac ttttaactta gcttctcttg
    54721 tttcatcgag aatgataatg actagctctt caaaactgat gtctgggaaa aaacggcttt
    54781 gccctaaata ttctttcaat gaatttccat aatggtttcc aaaaaaagta tccatgataa
    54841 aatgccgtcg atttctttct tccccttcga tccatgtccc ttgaccatgc ttagaaacga
    54901 tttttagtga atatggaatc aatcgctcct tgatttcttg catgtccttt gtcaaactgg
    54961 aacgactgat ataaagctct tctgccaaat catccataaa aagaatggca tcttccaaaa
    55021 gcaattggtt caatatatac ttttgtcgat cttcggactc ataaaatcct tgcggttctc
    55081 tagcatatcc aggtactatt tcttgccgac tcaaaaacaa gtcaaatgtc agtctttgta
    55141 aaatatgcag ttggtagcca taacctggtt tagcaatgat cttacctccg tttttttcta
    55201 ctaactcttt gatccgctgc aagtaattac ggattgtccg atcagataat aacaattcag
    55261 aagctaaacg ttgactggtg acatactcct tcttgtgatc aatcaaatat ctcaacagca
    55321 atttttcctt ctgtttcatt atcatccact cctattccat catatgttct cttcgatcac
    55381 ctctgctaac ttctggatgc ctgtagggat tgggacatac gcttggaaag ggatactaac
    55441 aacaggcttt ccaacagatt ctcctaattt tttgaattta tccaaaaaca tcgtcgtttg
    55501 cgggctgatc agaaataaat caaaatcact attcttaatc atattatcgc cttctgtagc
    55561 tgaaactgca tctacgagta cctcttctct tttttgctta aagaaatccg ttgtctttga
    55621 ggccatcatc gaggatgaca ttcctgccgc acaaataatc aatgcttttt tcataaatca
    55681 atcactcctt ttatttttat aaaccaagca tatcttgtaa gcgttttcta ttcaaaatat
    55741 atatttccgt agcattgcgg aaaaaatgtg tatcatatct tgaataataa tttctggcac
    55801 cgtgatatac tagtgataga attttttcag gaggggattt agtgagtatc acagtgaata
    55861 ttttgtatac aagtacaaat ggacaagcca gaaaatttgc agaagaaatg gtcgaaagaa
    55921 acattgtcaa agcgattcgt gcagaagaag gaaacgagaa atatgactat tactttccac
    55981 tagaagatcc agaatctctt ttactgatcg accgttggaa agatgaagaa gcaatcgaaa
    56041 agcatcacaa atccgaaatg atggctcaaa taaacgaact ccgtaaaaag tataaattaa
    56101 aaatgaaagt cgaacgtttc actgatttac cgtaaaaaga tacacatgga atgatagcaa
    56161 tttatcatcc atgtgtatct tcactttttc cttatttttt attcgatctc atctaatgat
    56221 tcaaaccgtg tttttctctt atttttcgga taagtatttc agtcacccca atatacatga
    56281 ttcctaaaca taccccaagg atattctcaa aaatgcggat cgttactgct cctggaaccc
    56341 cgaataaact ggcagtgatt gccaaagcac caaaagaatt aaaaactgtt ttatatctat
    56401 aggtagaaca aacacctaaa gctagaccac ctaatatacc gacccagtta aaaggaataa
    56461 attggctgat accaataaat aaaacacatc caatcaatga gccaatgatt cggtctactg
    56521 ctcgttcttt gaaaccaatc gacatcaatc catagctaga tacaatagaa gaaaatgcaa
    56581 accctgccca catcaatcgc tgaaaaggga catactctcc tgcaacgatc aaaagactaa
    56641 tgccaaatgc atagatgagc atccaaattt tttcctgcga atacatgcct ttcaaaaaaa
    56701 tctctttaaa caagcttttg tctttgttct tttctcgatg ttttctgtaa aggagaactg
    56761 aaaaccaaca aaaaaacaaa aataataagc tacttgtttg tgtaaagaaa ttcttgttca
    56821 acgaatcttt aggtaacgaa taaacaataa agagatatga aaagccgtac aaagaagcat
    56881 tccccatttt gggatcacta gcggttgcca ttaatatgct gcttagtaaa ataaaatgaa
    56941 tcaacatgcc cataactgac catttgatca gctggacata cggtgctact aatagtgaga
    57001 acatgacgat tgctaaacct atgatcgaat gggaaatctt ataaccaaag tcaacaaaac
    57061 gaatactcat caacatacag aataaaacca cagcgtatgg tttatgcttc gattcaaata
    57121 atgttgtaat tataatgata aaacaaatgg caaacaatac gatgaataat gatcgtaaaa
    57181 acaatgaaca aatatacatg cacttttctt ttttagaagc tgcttctcgt attttttgtt
    57241 ttaaaataaa cggatctagt tgtagagact gataaaaatc cacctgttgt cgtcctttct
    57301 ttttactttt attttttaca gtcatctatg acaggttgta aatactatgg aacatcatat
    57361 ggcgttgtca ttttcttgtc aactattttt cctattcatg tttttataaa aaaacatatc
    57421 gttttcttgt atgattaaat caccacaaaa taaacaactg agagaacgat atgctttatg
    57481 aatggttcta tcaaaaaaat tctgagatca atagacaaaa atttgatgat tacagaggtc
    57541 tcttacgaaa ccttacagaa gaaaaaaaca ttgatcgttg ctgcagttct cttccctgct
    57601 ccatgtgtct gtagaaattg tggttctgct gtggtagatg gaaataggaa attaatggtg
    57661 gtgaaaaaca agaagaagga aaccattgtc cactttgaat aatataatca tatgccaatg
    57721 gttatacgcc taaaaaagca gcgctatacc tgtaaaaact gtcgaagcca ttggaacgct
    57781 caaagttatt ttatccgtcc aaggcactcg atttcaaatc atgtcagaca taaaatcact
    57841 tctttactga ctgaaaaagt gtcgttattt tttatttcga aaagttgtta ggtatctttg
    57901 acaaccgtta ttcgcacatt gaaagagttg agaagctatt taccaaagtc ttccaaaaga
    57961 attctgtctt gagtgttgag ggttgtgaat ttcgttctca cgtttccgtt gaggataaac
    58021 tgagttttat ttccgcggat ggcgagacag aaaaattaat cgagaaaatt tacctacaca
    58081 aaaaattcct cgattaacaa actatttcct tagctgcact aatcctaaag aaatagaatt
    58141 cttagttaca gacatgaacg ccacctactt tcagctcacc aaacatgttc taccaaatgc
    58201 gaaattggtg attgatcgat tacttggttt ttccacgcct ttcaaggagg cgtaaccttt
    58261 attcacgagt tggttgaagc tttccgagac aaaagacccg acttgttttt ctccttattg
    58321 acaaagagcc attaaaacta gagaaaaaat tacaactgat cgctgattat ttgtagaaag
    58381 aaaatcaaag tattacccaa taaacttgag tcattaggca aaaaacactg cgaaactcaa
    58441 gaatgatttt cgcagtgttt ttgtatattt tttattaact agtattacta caaaggttta
    58501 tttttcactt ctcaacgcag cacaaaattg tctttactta tttactggtt gggttcaacc
    58561 ttctctactc cgatactaat atttgtttgt ataccccttc taaaatcggt agtttccatt
    58621 caattgatgg gtactagttg attttaaaaa ctatgcttat tcaatttttt attttagctc
    58681 atatatttcg tttctttcta aattttatac tgatgaaaac aagtagcaca cccaatattg
    58741 acaatataaa ctggctaatg atggtttctc cagtttttgg aagatgctta tttccactgc
    58801 ttgttggatt gtttcctctt atatctgagc tatcagcaac ttttctattc tcttttgtta
    58861 aaaagatcgc cacttcttgt tctttttcga tcttgaaagt tactggtgtc gcatccaatt
    58921 cataccccgt tggcgcttga gtttctacca gctgatatgc tcctggtgct aagccatcta
    58981 tcgccagttt tccatctgct cctgtggtta gacctgactg cagaacggtt ccattggcat
    59041 cttgtaactc aaaaacagct ccttgcagga cttctccgct ttggtcatcg atctttgtca
    59101 acacgacacc gcctggtgta agtcgatttt ctttcgtcaa ttctaccacc gctgtctgac
    59161 ttctttcgat ctcaaactcg attggtgtcg catccaattc ataccccgtt ggcgcttggg
    59221 tttctaccag ctgatacact cctggtgcta agccgtc
//
LOCUS       DAFEOM010000011        52463 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.011, whole genome shotgun sequence.
ACCESSION   DAFEOM010000011
VERSION     DAFEOM010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.011"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(<1..705)
                     /locus_tag="LP524_000810"
     CDS             complement(<1..705)
                     /locus_tag="LP524_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBL2233883.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFI"
     gene            1393..1551
                     /locus_tag="LP524_000811"
     CDS             1393..1551
                     /locus_tag="LP524_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2233884.1"
                     /translation="MNKKQTCEFLGIDNNTLDKWIRIYNFPVIRIDGIVRFDIEDIKD
                     FMNTFKTI"
     gene            1623..2849
                     /locus_tag="LP524_000812"
     CDS             1623..2849
                     /locus_tag="LP524_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL2233885.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISSALTHSDISTTRIYVNTPDVV
                     DKTVFEAFQRGLNKCD"
     gene            complement(2938..3330)
                     /gene="rpsI"
                     /locus_tag="LP524_000813"
     CDS             complement(2938..3330)
                     /gene="rpsI"
                     /locus_tag="LP524_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBL2233886.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(3346..3789)
                     /gene="rplM"
                     /locus_tag="LP524_000814"
     CDS             complement(3346..3789)
                     /gene="rplM"
                     /locus_tag="LP524_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBL2233887.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(4308..4775)
                     /locus_tag="LP524_000815"
     CDS             complement(4308..4775)
                     /locus_tag="LP524_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HBL2233888.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(4895..6433)
                     /locus_tag="LP524_000816"
     CDS             complement(4895..6433)
                     /locus_tag="LP524_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBL2233889.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            6513..7199
                     /locus_tag="LP524_000817"
     CDS             6513..7199
                     /locus_tag="LP524_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBL2233890.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(7400..7927)
                     /gene="msrA"
                     /locus_tag="LP524_000818"
     CDS             complement(7400..7927)
                     /gene="msrA"
                     /locus_tag="LP524_000818"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBL2233891.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(8011..8688)
                     /gene="pgmB"
                     /locus_tag="LP524_000819"
     CDS             complement(8011..8688)
                     /gene="pgmB"
                     /locus_tag="LP524_000819"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBL2233892.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(8685..9815)
                     /locus_tag="LP524_000820"
     CDS             complement(8685..9815)
                     /locus_tag="LP524_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBL2233893.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(9815..11998)
                     /locus_tag="LP524_000821"
     CDS             complement(9815..11998)
                     /locus_tag="LP524_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBL2233894.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(11995..13020)
                     /locus_tag="LP524_000822"
     CDS             complement(11995..13020)
                     /locus_tag="LP524_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL2233895.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(13065..13892)
                     /locus_tag="LP524_000823"
     CDS             complement(13065..13892)
                     /locus_tag="LP524_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBL2233896.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(13889..14956)
                     /locus_tag="LP524_000824"
     CDS             complement(13889..14956)
                     /locus_tag="LP524_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBL2233897.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(14982..15818)
                     /locus_tag="LP524_000825"
     CDS             complement(14982..15818)
                     /locus_tag="LP524_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL2233898.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(15815..16684)
                     /locus_tag="LP524_000826"
     CDS             complement(15815..16684)
                     /locus_tag="LP524_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL2233899.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(16754..18037)
                     /locus_tag="LP524_000827"
     CDS             complement(16754..18037)
                     /locus_tag="LP524_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBL2233900.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(18051..19736)
                     /locus_tag="LP524_000828"
     CDS             complement(18051..19736)
                     /locus_tag="LP524_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HBL2233901.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            20011..21036
                     /locus_tag="LP524_000829"
     CDS             20011..21036
                     /locus_tag="LP524_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL2233902.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(21631..25305)
                     /gene="rpoC"
                     /locus_tag="LP524_000830"
     CDS             complement(21631..25305)
                     /gene="rpoC"
                     /locus_tag="LP524_000830"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBL2233903.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(25363..28980)
                     /gene="rpoB"
                     /locus_tag="LP524_000831"
     CDS             complement(25363..28980)
                     /gene="rpoB"
                     /locus_tag="LP524_000831"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBL2233904.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREIFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            29708..30676
                     /locus_tag="LP524_000832"
     CDS             29708..30676
                     /locus_tag="LP524_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL2233905.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            30694..31599
                     /locus_tag="LP524_000833"
     CDS             30694..31599
                     /locus_tag="LP524_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2233906.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            31596..32402
                     /locus_tag="LP524_000834"
     CDS             31596..32402
                     /locus_tag="LP524_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL2233907.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            32538..33527
                     /locus_tag="LP524_000835"
     CDS             32538..33527
                     /locus_tag="LP524_000835"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBL2233908.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(33844..34368)
                     /locus_tag="LP524_000836"
     CDS             complement(33844..34368)
                     /locus_tag="LP524_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HBL2233909.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            34655..36493
                     /gene="scm"
                     /locus_tag="LP524_000837"
     CDS             34655..36493
                     /gene="scm"
                     /locus_tag="LP524_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBL2233910.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSTDTSA
                     SSDSTDTSSTTSDSTDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTS
                     NDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(36648..37634)
                     /locus_tag="LP524_000838"
     CDS             complement(36648..37634)
                     /locus_tag="LP524_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBL2233911.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(37657..38223)
                     /locus_tag="LP524_000839"
     CDS             complement(37657..38223)
                     /locus_tag="LP524_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBL2233912.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(38237..38653)
                     /locus_tag="LP524_000840"
     CDS             complement(38237..38653)
                     /locus_tag="LP524_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBL2233913.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(39110..41044)
                     /locus_tag="LP524_000841"
     CDS             complement(39110..41044)
                     /locus_tag="LP524_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL2233914.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            41325..42281
                     /locus_tag="LP524_000842"
     CDS             41325..42281
                     /locus_tag="LP524_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBL2233915.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            42371..43474
                     /locus_tag="LP524_000843"
     CDS             42371..43474
                     /locus_tag="LP524_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBL2233916.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            43487..44440
                     /gene="menA"
                     /locus_tag="LP524_000844"
     CDS             43487..44440
                     /gene="menA"
                     /locus_tag="LP524_000844"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBL2233917.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(45254..46600)
                     /gene="gor"
                     /locus_tag="LP524_000845"
     CDS             complement(45254..46600)
                     /gene="gor"
                     /locus_tag="LP524_000845"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBL2233918.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(46734..47987)
                     /locus_tag="LP524_000846"
     CDS             complement(46734..47987)
                     /locus_tag="LP524_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBL2233919.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(47993..48916)
                     /locus_tag="LP524_000847"
     CDS             complement(47993..48916)
                     /locus_tag="LP524_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBL2233920.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(49137..51629)
                     /locus_tag="LP524_000848"
     CDS             complement(49137..51629)
                     /locus_tag="LP524_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBL2233921.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(51636..52103)
                     /locus_tag="LP524_000849"
     CDS             complement(51636..52103)
                     /locus_tag="LP524_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBL2233922.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(52240..52313)
                     /locus_tag="LP524_000850"
     tRNA            complement(52240..52313)
                     /locus_tag="LP524_000850"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(52277..52279),aa:Arg,seq:acg)
     gene            complement(52371..52444)
                     /locus_tag="LP524_000851"
     tRNA            complement(52371..52444)
                     /locus_tag="LP524_000851"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(52408..52410),aa:Pro,seq:tgg)
BASE COUNT        15229 a        11070 c         9593 g        16571 t
ORIGIN      
        1 aataaatata gtatctccat aggctttttt taaattctct tcattaaata cagattctac
       61 acttccgtga gcaattagat ttcgattcaa aagaataata ttatcaaaat acttctttac
      121 cttgcttaaa tcatgatgca caattagcac tgtttttccc tgatgtttca attcttttaa
      181 cgtatccata attattcgtt cactaactaa gtctatccct acaaatggtt catctaaaaa
      241 tatgaaatcc gcattttgag caagacatct tgctaaaagt acacgttgaa attgtcctcc
      301 agataattct cctatttgat gattagaata ttttcccata tcgacctttt ccaaagcctt
      361 tgaaactctt tgccattctg catttttaat tctttgaaaa actttcattc cggcatatgt
      421 tcccatagat acacattctt ttacttttat tggaaaagta aaatcaatat cagctttttg
      481 ctctacatat gcaatcttca ccagtttttt attgatatct tcattattaa tggagacctc
      541 tccctgatga gggataatat ttaacattgc tttaagcaat gtagattttc cagcaccatt
      601 tggaccaata atacctgtta aagcaccttc ttgtattttt aaagaaatct cgttcaatgc
      661 taaaaaatcg ttataacaaa cattaagctt tttcacttct aacataactt tctccttcta
      721 aatgataatg attatcacga tcatatggta tacaaaaatt atattttagt caaccctaaa
      781 ttttataatt tagatttttt tctgttgctg aagattagaa tgacaaaact atttgaaact
      841 acgcgtatag taagtagctt tcaaaatata taaaaaagga gcgttctcaa atgagaacat
      901 gctcctaaaa ctactttatt ggcgtatgga aaaattatac ttaaagtgga tagcgggttg
      961 aataagttga taagcatcaa aacatttata aaaattattc aagactatgt ctgttccata
     1021 tacaaatatg aaaatcagta aactccattt aacttttttc gtagtgtgtc tttttataca
     1081 tgtttatcgt tattaatagt gaataggaaa aaacctattt ttgatcattg acaattaaat
     1141 acaatttttg aataacgatc cctgctattc gagctagaaa ctcaagtacg ccatgacctg
     1201 attagcgaca aatacttaga gatttctttt tgttctggga aaacaaaatg ccatgacaat
     1261 agcagcaaat aatgatactt ccgtaaggct cgccgtaaac tggggagagg tgaaattcct
     1321 atgtgcttac ccaaagcaat tcaaaaattc taatcttttt taggagatga ttttataaaa
     1381 ttgagagtct acttgaataa aaaacaaacc tgtgagtttc taggaattga taataatact
     1441 ttagataagt ggattcgtat atataatttt ccagtaatca gaattgacgg tatagttcgc
     1501 tttgacatag aggatatcaa agattttatg aatacattta aaacaatttg aaaatttctt
     1561 ctcgccgcat atactaagaa tagcgaaata tactgcctta ctttttaagg gagtgaggaa
     1621 ttttgcctac aaaactttca aatgggaaat ataaaactaa tctgaggtat ccaaaaaaat
     1681 tcagagaaat cactggaatt acatcagaaa agtatcaaaa agtatttcca actaggcaat
     1741 tggctattaa agccgaaaat gcaatgaaaa ggaaaattga aacagtttta agagaggaga
     1801 atgcaaattc actagaatta aaaggaaaaa taaaatttaa ggaattctat gaatcaaaat
     1861 ggcttcctag atatgaacta ggtcagacta ctagatcaaa aagagctcca agttacgtaa
     1921 caattagtaa tactaaagat atattccgat tgcacatcct tccaatgttt ggtgagtatg
     1981 caatgaacta tctaaattca aatactgaga taatatcaga tgaattaaca aaaaaatcga
     2041 aagaatacgc aaatataaaa attatcaaag gatatgtccg ttctatgttt gatattgcag
     2101 aaatactaaa ttatattgaa tttaaccgaa ctaccaaaat tattcaatgt atcactgcac
     2161 ctaaaaaaat tgctttagaa gaaagaagga tacgggaagg taatcaagca ttatcgtcag
     2221 aagagcttat tgcttggata gaagcagtaa acactgactt gaataatcac ttgcttactc
     2281 ttcatgacta tactcttttc atgcttaccc tatatcttgg tgatagaaaa agtgagacgt
     2341 atgctttgca atggaaatat atcgactttg agaaacaaac ggttcgcctt aaacatgctt
     2401 tggataaata tcaaaaaaag aaatttacaa aaggtcgaaa ggataccgta attcaagtac
     2461 ccgaaattgt aatggcatta ctaagcaaat ggaaatcagt gcaagctgag caattgttga
     2521 gattaaaaat taatcaaaca ccagatcagt atctctttac ctatactaaa ccatccggag
     2581 aagtaaattg ccctgttcac gctgattacc taaactatcg aatcaattca attaaaagac
     2641 gacatcctga attggctcat ttatctccac ataagcttcg tcatacctat gcaactatag
     2701 ctagacaagg cggagcaaat atgaatcaaa tatctagtgc tttaacacac tcagatattt
     2761 ctacaacgag aatatacgta aatacaccag atgtagtaga taaaactgtt tttgaagcct
     2821 ttcagagagg attgaataaa tgtgattagt atgtgttctt aaatgtgttc ttaaacaaga
     2881 aaaagacact tttcaaacaa ttgaaaagtg ccttgaaaca ttgatatata aataatatta
     2941 acgtttagag aattgagatg ctttacgagc ctttttgaga cctggttttt tacgttcgac
     3001 catacgtgcg tcacgagtaa gtaagccagc acgtttaaga gctaaacgga agtcaggatc
     3061 aacttctagc aatgcacgag cgataccatg acggatagct cctgattgtc cagcgtatcc
     3121 tccgccgttt acgttaacaa atacgtcata tgcgcctttt gtttcagtaa cagcgaaagg
     3181 ttgattgatt acttcacgca agtcagcatg tggaatgtat tcttcaacat cttttttatt
     3241 tacagtaatt ttgccagttc ctggtactag acgtacgcga gcaactgcgt ttttacgacg
     3301 gcctgtgccg atatattgaa cttgtgccaa tgaaattccc tcctattaga ttaagtttgt
     3361 gatgtctaaa acttctggtt gttgtgctgc atgtggatgt tctgctccac cgtatacgtt
     3421 caattttgta aattgtttgc ggcctaaagt gtttttagga agcatacctt ttacagaagt
     3481 ttcgatcaaa cgacgagaat ttttagcacg caattcgcca gcagtgattg atttcaatcc
     3541 tcctgggtag ttgctgtgac ggtaataaat tttgtcagtc gcttttttac ctgttaattt
     3601 cactttgtct gcattgatga cgatgacgaa atcaccagta tccaaatgag gtgtgaaagt
     3661 tggtttattt ttaccacgta gtacagatgc aacaactgtt gaaagacgtc ccaatggaac
     3721 atcagttgcg tctactacat accatttacg ttctacttcg cctggtttgg ccatatatgt
     3781 tgtacgcacg ttattttcct ccaatttaag ttctgatgtt tgacatacac ttcgagtttc
     3841 cggggctctt tgtggggcaa acaataccat ttaacatatt acccgctaat ctagtcaatg
     3901 tcaacgtttt tctgcttgat tgaaaaattt atatgaaact ttacttcttt tctttctttt
     3961 atataaaagg gtcgtttcct gtcaaaatga aaaacaaaaa gaggctgtga caaaagtctt
     4021 gtcacaaccc cttattttga ataaacggtg ttctcctgcc aactcctcgg tcacaagtca
     4081 caatatccat aaaacataag aagctgtttt ttaaaattgt ttcttgtgac tcggagctga
     4141 acggcaggct tacaacctct tggtaaacgg tgttcaatca gctgaccctc ggtattagct
     4201 caaaaaacgg caaaatatga ggaactattt tgacgtttgt ttctctaata ctcaggtcaa
     4261 aacgctgatt tcacaacctc tttttcggtc aagttcaaca acagttttta cgcgtcgatc
     4321 tctggcgctt tgcccaaatg agcttggatc atccagatac gtttttctgt ggctgttttg
     4381 aattcgataa agatatcttg tgttggatcg tcgccttctt ctccagaaac ttcgattcct
     4441 ttttggtaaa gatctgctag ataacgatag cctgcaacaa gtgtttccaa acgttctgga
     4501 atagtacgat cccagcggcc aatttcatca gggatttttg tgttatctgc aaattcacgc
     4561 aatgtagaat aaggtgaacc gtctaatgtg atcaagcgtt cggatacttc atctaactga
     4621 gcgttcaaat catccatgaa ctcatccatc attgggtgca atgtaaggaa acctggtcct
     4681 ctcatgtacc aatgtgtttg gtgaacaaca acagacattt ggcttagatc tgcaactaat
     4741 tgatttaata cttctttggt ttgttggaat ttcatctttc ttgcctccta acattttctt
     4801 acacttaaaa taatagtaga ctttcaatga aaagtacaac aaaatcattt acctgagaac
     4861 gattttcagt tcgctttttt atccgtaaag ccaatcattt ttcagaatca gctaattctt
     4921 ctcgcagttg agcaaattgc tgataagtct gtgctaaatg cttttgtgtc gaaacaaata
     4981 atggatagat tttctgatat tttttatgat cagtttcttg tactccaact tgctctttca
     5041 attctatcaa ttcctgtgct tcactgatac tttggatctt tccgatgctt ctccagccta
     5101 aaatcacagc tcctaatgcg gatgcttctg tgacagtagg gaaaacaagc gggtaaccca
     5161 gtacatcagc catcatctga cgaaaaatcg gagattgagc aaacccgcct gtagcacgga
     5221 tctctgtgag ctgattttcc tggcaaactc cttttaaaat ccgataaagg ttgagacaga
     5281 tcccttctag aatcgccttc atcatttctt tttctgtatg ttgccgctgt aaaccgataa
     5341 aactgccaaa cgcatctgcg tcccaaagcg gtgctcgctc accaagtaaa tagggatgaa
     5401 aaatcagacc attggcaccc gctggcacaa aagcgatagc cgaaagcagt gcatcatatg
     5461 gattttctcc tttttcttta gcagattgaa cgacttcctg taaaaatgta tgccctgccc
     5521 aatcaaagat gccagcacca ttgctagtcg ctcctcccac tacccaatgt ttctgatcca
     5581 gaacataaca aaacgtctcc atttctggat ggaactgagg ctgagtagta ataaaacgaa
     5641 gtgctccgct cgttcctacg gtcaaagcgg cgatcgtatc accaaaagca cctaacccta
     5701 gattagaaag cggcccgtct gcccctccca aaacaaatga tatatcattt ggcaaaccta
     5761 gttctgctgc tgtttttgat ggtagacctg ttacttgata ggtggaagga aagacttgcg
     5821 gcaattgctc tattcgaatg ttcaagtagt caagcacttc ttctgcccat tggaattcat
     5881 ggatatcaaa atatccagtt cctgaagctg ttccgtagtc gctgatcagt tcgccaaaca
     5941 atctataaaa gatatagtct ttgataccga caaaataagc agtttttttg aataattcag
     6001 gttggctgtt ttgcagccac ttgattttgt tcaatggtgt cattggatga atcggcgtcc
     6061 cagtctgccg ataaaattct ttgccaagca cggtatattt cagtttttct gcttcgtccg
     6121 ctgctcggtt atctgcccaa gtgatcattc gagataacgg tgttttctct tcatccaaca
     6181 atatcaggcc atgcatcgca gaggagaagg agacaaattg aatgattgtg tctgaaggca
     6241 actgtttaat tacttctttt ataacggtgc agacaccttg aaagatcaat tcctgatctt
     6301 gttctgccat cccttgtgtt tcttggatca aagggtagcg aaaataagcg gatgctgctt
     6361 cttgggcttc atctgtgaaa gcaactgctt tggtttgtgt ggttccgata tctaatccga
     6421 ttgaaacgtt cattttttca cctcaaattt tatgtatgta caaaaattta gtttaagctt
     6481 gcgtatcttg gttttgaaaa gggggagatg gcgtgttact ttattttatc attggttgct
     6541 gtatcggatc atttttatgt ttaaccgccc agcggattcc gttaggtcac tctattattt
     6601 atcctagatc tcattgtgtc aaatgctgtc gttcgctttc ttggtatgaa ttgattccga
     6661 ttctctcgat catcgtccaa cgatttcgct gccgctattg caggtgtcgg ttgcccttct
     6721 actatttgtt ggcagaagcc ttatgtggtg gtctttttgc ctggttcttt actttttctt
     6781 ccgcaagaaa tttttcgacg ttcatatgga tgctttcggc tttgctattt tcgttgatgg
     6841 atttatttta cttcatggtc cattctcaca cgctattctg ttcttgggca attttatgga
     6901 ttttttggtt gcaggccggc gtcttccaat ggcaaagtgt gctgttaacg tttatcgtag
     6961 gcggtgtgtg ccttcgctat ggtcaatcct atctcggagc tggcgatact ctgttgatgc
     7021 tttcttggag tggtggcttg tctctagaag agttgcttca gatcttgttt cttgctagtc
     7081 tgtttggaat atccgttttt tctgtttact ttatctgtca taaaaaaaag ttggaaaagc
     7141 tgcctttcat tccttttttg agcagtgcgt tgctgattgt ccttcatctt aggtattgaa
     7201 aaaaggttgt gacaaacgac ttgtcacaac ctctgatata tagtgttctc ttaccaaatc
     7261 cttgtgactt ggagctaggc gacaggttca caacctctcc actaaatggt gttcaatcag
     7321 ctgaccctca gtattagctc aaaatgctgg ttttacaacc tctgatatac ggtgttccat
     7381 aagtagcttt ttcggcttct tatttcttga cgccccaatg tcgacgaagg aaacctgcac
     7441 gtccagaacc ttctcgataa agattgtaat ggagcttgtt tttcttgtaa taatcctgat
     7501 ggtaatcttc tgcaggataa aattcgcttc ttggttcgat ttttgtcacg attggcttat
     7561 cgaatcgccc gctgtcttgc aatgcttgtt ttgaacgctc tgcaatattt cgttcttctt
     7621 catcattata gtagatcact ggacgataag aatcaccgcg atcagcgaac tgtcctaatg
     7681 cgtcagtcgg atcggtttgc tgccaataga tttccaccaa ctgatcataa gaaataactt
     7741 ctggatcata ttcgatctct actgcttctg tatgccctgt tgtgcctgtt gttacttgtt
     7801 cataagttgg atttggtaca tgcccgccag tataaccgga tgtcacagaa atgatccctg
     7861 gctgctcttc aaatggtttg accatacacc aaaagcatcc gcctgcgaaa actgcttttt
     7921 ttgtcatata aaatccccct tttttctcat cataccttga tttccaaaag gaggataacg
     7981 ttttgcttgt gacagaaaaa acggaaacta ttatatacgt gatacggctt ctgataaatg
     8041 ccaaaacgaa cgtacttgat caaaaacggt taaatcagga atcacatatt cagcagaagg
     8101 aagatttatg ttttcaccta ttcccaatac atgcatgtta ccagccaaag ctgcttgaca
     8161 accagcttct gaatcttcaa tgaccaagca tgcatttggc ggtacattca attgctgtgc
     8221 tcctttgaga aaaacttctg gatcaggttt tgcttttgat acttgcgtgc cgtcaataag
     8281 gacatcaaaa taacttgtca aattcgtttt ttctaaaatg aggcgtgcat ttttgcttgc
     8341 tgatcccaat ccgatcttga tgtttgtttt tttcaagtac tcaagaacag ctattgcacc
     8401 aggcaaaacg gatgtttcat ccattttttg aatcgcttga agatatagat tgtttttttg
     8461 ctcagcaaag gctgatttct ctttttctga gaatttctgt tccatttttc caaatgacag
     8521 caatcgctct agtgactcgg ttctggatat tcctttcagt tgctcgtttt ctttctcagt
     8581 gaatggaatg gacagctggt tagctaaaac cagccacgct tcgtaatgat aacgagctgt
     8641 gtctacaaga acgccatcta aatcgaatag tgctgctttg attttcatcg attacctcct
     8701 tgaaaatgaa cgacttgaag taaagatgcg atgtttttag ctgtgcgatg aagattatca
     8761 gtggcttgct gtaaagcttc ttgtaaaggc acagctgcag gaaggatcgg aaaaacggcc
     8821 gaaatgccaa catctgctgc tttttcgctt cctttaccaa cacttccgca aatagctatc
     8881 acaggaacgc cctctggtac tcttctagca atcccaactg gcgcttttcc ttttagactt
     8941 tgtccatcta gacgtccttc gcctacgatt acgaggtctg cttgctgtac ccgtttatcg
     9001 aactccagta aatccaatac gtagtcgatg ccagagacta ttttccctga agcaaatgct
     9061 aacagtcctg cacccattcc accaccagca ctactgcccg atgcctcgat tacttgaggg
     9121 gcaaatctca tataaaaatc atgcatggct tggtcagcca ctgctaattc attttctttc
     9181 aagccttttt gtggtccgaa aacaaaagaa gcgccttctt ttccgcataa aagattagta
     9241 acatcagtaa tgacagtgat tttggcttta atcagctcaa taggcacgtt cgttttatca
     9301 actgtagcaa ttttacctag attttctccc agtgcttcta ctttttgatt atcttgatcg
     9361 taaaaagcat aacctagacc ttctgccatg ccgatgccgc catcgttggt actacttccg
     9421 ccaacgccga tgatgatgtg tcgaacacct tgttgcaaca attgtcgtat cagttctcct
     9481 actcctcgcg tggataacct aagtggctgt tgttggttta gtgggacatc tttcaatccg
     9541 acgatttctg ccatttcaaa aatcgcagtt tgaccgtctg aagcgtactg gatttctttg
     9601 tttttgccaa atgcattagt aacgactgct gtttgccgct tcaaccttaa actgtctgct
     9661 aatgatgaaa ccgttccttc tcctccatcg cctataggta gcagatcata ttcagcctga
     9721 acaaaaactt cttgaaaacc tgattgaatg gcttgtgctg cctctaaggc tgtcagactt
     9781 tctttgaaag aatctggcgc aatcagaata cgcattattt tcccctccgt tccaagatca
     9841 gttctttttc cagctggtat tggtctccat tgacaaaaat aggtaatgtt tctttcgtct
     9901 ctttttttat tactatttgt ttttttgtca aaacaaatag caacctgtga tttttccaaa
     9961 taaaaggaaa agaaattttt tcccatgctg aaggaagtct tggagaaata gaaagagttc
    10021 cattcgttac tgtaatacct gaaaaaccaa atataactgc tagccatagt gctcctaatg
    10081 aagcagcatg aattccgtca tctgatgaat gcggatctgg tcctaaatca atcaggcacg
    10141 cttcttggaa taaatgatac gcccattcca tttcttggat acggcaagcc tctatggcgt
    10201 gaatcgcttt actcaacgat gagtcgtgga ttgttttgtt ttcatagtaa tgcaaattct
    10261 tagaaacgat tggttgagaa aaatactcag gtaataagta taacagcatg acaagatctg
    10321 cttgcttgct gacttggagt tcatttactt cgctacgggc ataatcaagt aaaatagact
    10381 ggctgccagg acgttctttg taagtggtca aatcaataac tggcttactg agatacgtgt
    10441 catcttgcgg aatcagctgt tctttgttag gcactggcaa atataactta tctaaaaaat
    10501 tttgacattc tttcaaaaaa agcggatcgc cagcttgata tttaacagct aaggccacat
    10561 tataatgcgc cagatagttc gtatatgcat tattgtcgat atattctgtg tattcatctg
    10621 gaccaatgac tctgtgtatc tctaaatgat catcgacttt tacagctctg ctcagccaaa
    10681 aagctgctgt ttcatgaatg agcgtttttc cctttgtttc taaaaacgat tgatcctctg
    10741 ttgcttggac atactcttga acggcataag caaggtctgc actgatatga tgttctgcgg
    10801 cagctgaagc aacttcttgt cttgttccgg ttttgatatt caatgcagca aatttgggtg
    10861 tctcttcctg accagtgtgt gcgctttccc atgggaaaag tgcaccttga tagtgataat
    10921 ctcttgcttt ttgccgtgcg ccttctaatc gatcataacg gtacagcagc aaattctttg
    10981 ctacttcagg aaactgatat aaaaagaaag gcaggataaa aatttccgta tcccagaaaa
    11041 catgcccttt ataaccttcg ccagttagtc ccttcgcccc tacactaaca cttggatcat
    11101 tgcggggagt cataatctgt aaatggtaag cagcgaagtc catcgctaac tgatcgaaag
    11161 aagaatcgcc ttggatcgtt atccgatgct gatgccaaaa atctgtccaa taagacatcg
    11221 actgctctaa aatttctgta taactaacgc ttttctgggc ttgggcaata cttttcttaa
    11281 acgggtctgt ttgctgaaga gaggtagaga gcacgatgat cttctccagc gtaaaggacg
    11341 tattttttct gatcttttct gaaaaggaag cggtgatccg gcgatttttt gcttggaata
    11401 cacctgcttt attgaattta gctgcaatcc caatatcaat tttactttct gttgtctggt
    11461 aggatgcact gatcaaatcg tcaccaaata ctcgcagttc tttttcaacc aactgctgtg
    11521 taccaaaatt ggtttgctgt ccatcgatac ccgttttcac atctacttgg cagtcttcgg
    11581 tcaacggctt gatgaccact ttcagggctg cctgatggag attattttga tcagcaacac
    11641 gacaagtaat gacttccact ttttttcctt gagcagtttc aatgagcgca tagcgtttaa
    11701 gctctcccat ttctaaatct agtgcccgct cgtatgcaag aactttcatc gtctcaagag
    11761 aaaaatgttc accgtcgatt tttatttccc atctgatgac atctgggaga ttcactaact
    11821 cggctgattc tgctcctaaa ggtcggttat atacaccagc tgcaaacatt ccccttactt
    11881 gattccgata gtcttcctcc tggcttcctc tgataccaaa aaatccattt ccaatggtca
    11941 tctgactcgc atacttattg atatactcta aatcaaactg tttttcttct actttcataa
    12001 ggtaataact tctcctgttt ctgccgaacg atataaattt tccaccagat tctggatgac
    12061 atatccttgc cagccatctg caatcatctg agtctggtca ccgagcgccc gattcacaaa
    12121 atgagccata cttagttcat gtgggtcatt aactggcgtg gtcttttgta aggaaaccag
    12181 ctttccatct acatcttcga atagttcgcc tggatacaga ttagcacctg cctgatcgcc
    12241 acaaaaagcc acattcagtt tgtcttctct gatattcaaa gcaaacgaag tattcagttg
    12301 gatgatcttg ccgttttcta attccagtgt gccaaaaatt gcatcttcta ctgtgaattt
    12361 gcttggatcc catttcccga atttccctga attttttttc gtaccaattt tttggaagct
    12421 gtacgcccga acgcttttta ctttaggaaa tccagtaata tacatcgctg tatcaagcat
    12481 gtgaatgcca taatcgatca aaggcccgcc tccttgaagt tctttgtcga taaacgttcc
    12541 ccagcctgga acaccagacc gacgaagcgc attcgcttct acgtaataaa tatccccgag
    12601 aacatcaaga tgattcttga tcattgttgc ttcgtcagaa aaacgatgct ggaatccata
    12661 agccaaaatc tgcccgtttt tatcggctgt ctctgccatc tttcttgctt gatcagctgt
    12721 catagctggt ggtttctcgc acatcactga agcattagct tcgagggcag ccaatacatg
    12781 ttcgaagtga aagcgattgg gtgtacagac actcacgata tcgggagaaa tctcattcag
    12841 catttgtgta actgatgtat acgcatgaga aaatcctgta cgtcggcaaa agtcttctgc
    12901 tcgctggctg actggatcaa ccacagcggc caattctaag acatctgatc ttttttgata
    12961 ataagcagca tgtacttttt ctgctacttg tccagcgcct atgattgcta ctttcttcat
    13021 ttatttctct ctcctttgct tatttactta ttgttattct ttctctattc tttctctaaa
    13081 tcatttaaat gacgcgctgt ttccagacag tttttcaagt attgcaatga ttcctgataa
    13141 attttcactt cattttctcc tcgcaaacga cattcatata ccatataacc ttcatactca
    13201 ttttgaagta gcgtttgaaa atggcgagta aaatcaattg cgccagatcc tggttggtag
    13261 cgatgattat ctgctaaatg gatatgaccc acatactctt tttgcgtagt caatgctttt
    13321 gaaatatcat cttcttcaat attcatatga taaaaatcag cgatgatctg cacatgtttc
    13381 aattgattag agtcaatgta atgacgcgca tccgctaaca aattgatcat atggtcttgg
    13441 tatcggttca aaggctccaa ataaataacc gtacctgttt cttcggctac tttatctaaa
    13501 taacgaagtg attcgctgac tgctttatag tctccttctt tgcttcgggg tgaaaccatc
    13561 ggcggtaatc gataagtgaa cattccccaa gctgctggga taacgattcc tttgcctcca
    13621 acctcgctta gtgcacgcaa aatctcagca atttgacgta caccattaag acgccgttct
    13681 tcgataaagt caccgatcca cccatcgtag ccgttacaag ctgtcactac tgggatccct
    13741 gtttctttga ttgcctgttt gactgtgtcg atattttcta ttaacaactt accgtcgatt
    13801 tcgtattctt caaatccaca gtctttgata aatctaaatt tttctaaaat gtctcttggg
    13861 aaaaatgctt gatcttgcgt accgaatttc attgttctcc tcctcggact tgaacgccca
    13921 ttttgatact ttcttctggg tgctggtcta cataacgcat gtaagcttct gcgctttctg
    13981 caaatggcac aactggatca atgatttctt cacaatccaa gtaaccgttc atcaataatt
    14041 cccaacatgt gtcctcgatt cgtttacggt tccatcttgg gtaatctggg tttggttcac
    14101 tagatgctcg agcaaagacg atttttgcat tattgaaatg tgcttcgcga ccaagattaa
    14161 acccagctgg aaaaggttta gcaaaggcaa cataagaaat gatcccgcca tagccgatcc
    14221 ctcttaaaga agcttgtaat gcggcagcac tgccactcgt ttcaataact gaatctactc
    14281 cacgtttatc tgtcaattcc tttagacgaa aaccgacatc tgctttcata ggatctagcg
    14341 tgtaatcggc accgtatttt tctgctaatt ctctacgctg cgctatggga tcgatcccaa
    14401 cgatggtagt agcacctgct tttttagcta attgaatcgc aatttgcccg atagcgccta
    14461 aaccgaagat tgctacagca tcacctgctc gaacatgtgc ttcccggata ccacctaaag
    14521 cgaattgagc aggatcgtaa catactgcat ttttccattg atccaaccga tcaagtttac
    14581 gtagtttata attatcaaca ccgtttgtaa tcagcgtttc acgaatgggt ccataagtgc
    14641 agactaactc tcctttttga tactctgtca ccaaactgcc cgtttcttcg atcgtaccga
    14701 caatcatatt tcctaattga aattcgccaa ataccacacc gcgttggctt ccttcttctc
    14761 ttggcaagaa taggcgccat tcttctgaaa attcttcatc gataaatgga cttaatcctc
    14821 taaagtcgac aatttctgtt cctggttttg gagtagcgaa aattactcgg atacgtactt
    14881 cgttcttttc gatctcacgg tcttcgtaat ctttgaatcc ggctattctt ggttctaatg
    14941 caactaattt tttcatcttt tctttcctct ttcttttctt tttatccttt tacgccgcct
    15001 tcgactaaat cacctttaat aaatcgttca gataacgcat acataatgat gactggtaaa
    15061 gcggtcacca atgaagcggc catcattcgt ccccaaatgt agtctggtgt actgaataat
    15121 gtgttcaaac cgattggtaa agtaaatttt tcggaggtcg acaggaaaat cgaagcaaat
    15181 aaataatcat tccacgaaac cataaagcaa taaacgaaga cagaaatgat tccagaaata
    15241 gccaatggta taatgatccg aataatgatc tggatccggc taagaccatc gatcattgct
    15301 gcttcttcaa tatcagtcgg aatcgtgtca aaataacttt ttagcatgaa tatcgcagta
    15361 ggaagtgttt gaacgaccat cgtgataatc agtgctgtat tggtatcata caaacctaga
    15421 ccggaaatga ttttaaataa tggaacaacg atcaaaatac cggaaaacat ataaacggtg
    15481 taaaagctag cattgatttt cgagcgagct ttgaagcgta gttttgacaa agcgtatgcc
    15541 ccgctgatcc caatcactac tgcagttacg gaggctatca aggagatcac taaactattt
    15601 ttgaaataca tgatgaatgg aaaaatctca gcattgaaaa tatcctgata atgttcaaaa
    15661 gtccattctt tcggaaaaat agtttgtgtc gttgcaattg cttcttgtga atccttgaat
    15721 gatgtcatca gcatgatcaa aaatggaaac aaattgacag ctaatagcaa caaaatcgtc
    15781 gcgtaaaata agattttcct gctcttttta tttttcattt caagtccacc ttctttcgtg
    15841 agagcaagac gaccgctgca ataatgatga acaatagaat cgatatagct gctgcttttc
    15901 caagatcatt gaacgcaaag gctgtctgat acaaataaac gcctaagata ttgactttat
    15961 tcgtcagtag atagacatct gtaaacatat agaacatcca gatagatcgt aaaacaatca
    16021 cagttgctag tactggtttg attgccggca gtgtcacgat cttgaacttc tgccaagcat
    16081 ttgcaccgtc catttcagct gcttcataaa gcgttttgtc gactgtttgc aaaatagcca
    16141 aaaatgaaat aaacgcatac ggatagtatc tccagatagc aaacaaaacg accataaaga
    16201 agctagcaac tggctggtcg aaccacagcg gtgcttcgtt gaaaagctgc aacctatcta
    16261 ctaacagatc attgacgatt ccataactat tattgaacat atatttccaa gcaaaaatca
    16321 aagaaataga tggcgtaaca taacttaata aaatcagtga acgcaccgtt ttcctaaatc
    16381 tgaacttgcg gttaagcaaa atagctacgg ctaatcctaa agctgtactg cctgctacaa
    16441 ctaagaatgt gtaagcaact gttaaaccta aagaacgata aaattcacta tcacttaaaa
    16501 tttcaagata attgcttaat ccgataaaaa cggcgtctga gttaggattc agcggcatat
    16561 cgaaaaaact gatctgaata ttggaaagca ttggaatcag cactaaaaca gctaataaaa
    16621 gcatgcttgg tgctaaaagt cccatggcta attttaagtc agaatgtttc cttggacgat
    16681 tcattgaaac ctccccctga taatctgcca atagctatct acttttttcg aacaccattg
    16741 gcagaacttc ttgttatttc gtattttttt gtgcgtcagc taaagcttga tcgacatctt
    16801 cgtttccgac agttacttgg ttcactgctt ttccgatcac acctgagctt gtgatatctc
    16861 ccattttcgt gaaattcttt tccccgacta gtccaaagac ttgtacttca tcaaaagcac
    16921 tagcaatctc agatggcagc tcgccaaaag acttgatgac ttcattttct ttgtaaccat
    16981 ccagttcgac tacttgttta ttcactggtt gagcaccacc aggagacatc aagacccact
    17041 tttccatgtt tttcggttga gataagaaag ctgtgaatgc ttcagcagcc tctttttgtt
    17101 tctcgtctag accagaagaa atcgtcaatc cagatactgt gccatagaca gcttgatctt
    17161 tttgtgttgg gatagcaaaa ccgatatttt ctgagttccc cgcttcataa acagaaggca
    17221 aaatgtaagt tgaatagatt gccattggtg ctgttccatt catgaaagca tctttgattt
    17281 ctgtcacatc attagagcct ggcatcgtgt attgggataa gttttgataa aacgacaatg
    17341 cttctctcat cttttccgta tcgatcgtta cctcgccttc atcatccaaa acatttgctc
    17401 catttgacaa agcaaattga gagaacgctt gctcactcat tgttccttca gcagtaggca
    17461 tagcaattcc atattgttta ttctctttat ctgtgaaatg ttgagcaatt tttaaaatat
    17521 catcccaatt ttctggttct gaaaaaccag cttctgataa tttttctttg tcataccaaa
    17581 taccttgaac ccagccgcta attggtgcag caatatagct ttttccatct tctgaacgaa
    17641 caaggttttt ggctccgtca taataattat cttctccagc ttcctcgata acgttttgga
    17701 ctgccgtctg atcaatcagt tcatctttat ccatcacttt agcaaaatcc tgactgactt
    17761 ccattacttc tggtaattta ccggaacgag ctaacgtaac gatttttgta ttgaacgctg
    17821 attcttctac cggcacttgc ttgattttga tgtctggatt ttctttttca aaatcagcaa
    17881 ccagttcatt gatcacactt aatcgttcct gttcgacaga agagtgcatg aattgaatcg
    17941 ttgtctgttg tgattcgtta gagctttgac tttcaccaca agcagtaaga agaaatccag
    18001 tagctagcag acaggtagta acaataattt ttttcatttt tttccacctc ttattttatt
    18061 attttcagcc acatatattc atacggctgg aatatcaact ttcgttcttg actgcaatgg
    18121 ataatttgtc cagaaaacag gtctaggtaa tctccttttg gtaaaagata acctgctgat
    18181 tgattggaaa aattatggat aactaagact ttctcttcct tcgcttcgcg aataaaactg
    18241 ataaaagaat ctgctgagtc cagtaagtaa atcaaactat tcggatggaa ggctttttct
    18301 ttttttcgaa tagatagcat ctgggtcaat tcaccatata ctgctgttct taatggatct
    18361 gtctgcaact ctttctcaat atctgctaac aaaaattttt gccgattgat ttttcgattc
    18421 tctcctgttt tttctacgcc tttgtaatca tttcgacttc ccagcacact ttgaatataa
    18481 attgccggca cgcctggtaa cgttaccaaa aatgaatgag cagctaaaaa gcgtttgctt
    18541 ctccattccg tcgtatctgt tggagaactt aacgcatcta gatacgtagc gtttatctca
    18601 tatggactgg tcgttccatc tgggtttttc ttgtagttta ctcgggcacc ctctttcttc
    18661 agctgttcca ctaattcaag gatcgttttt tccggcagaa tacctcgtac cggattcaat
    18721 ccgataccgt catgggaggc taaaaaatta aaaaaagttg tttttttcga tgcgaaatcg
    18781 atggttttta accattcttt caacttactt gtattacccg aacgaatcgc ttgaagggtc
    18841 aaaggtggta gcgaaaactg atagaccatc tgtgcttcat tctcgccatt cccaaaataa
    18901 ctgatattat ctttgtgtgg cacatttgtc tcagtgatca ataccgttcc tggagctgct
    18961 tcatcagcga ttgcacggaa taattggatg attttatgcg tttctggcag gtgtatacaa
    19021 gaagttccta gttctttcca taaaaatccc acagcgtcca agcgaataaa tgaagcgcct
    19081 ttttctaagt aaaacaaaag aatatcgatc attttcaata aaacttctgg atttttaaaa
    19141 ttcaagtcta cttgatcttc gctaaaagtc gtccatacgt gtttttcttt cccgtttttg
    19201 tcaatgaacg ttgtaagtaa cgggctggtc cttggtctag tcacgctgct tgtatctgta
    19261 tccagtggca aatcaataaa aaaatctttg tacgtatcat tcaagtttaa atattcctga
    19321 aaccaagtac tttcggcaga catatgattg cagacaaagt cgaacattaa ccgtgtttcg
    19381 cttctcattt cttctatatc ttcccatgtg cccaaacggt cgttgattgc ctgataattt
    19441 acaacggaga aaccatcatc agatgtatat gggaaaaatg gcaagaaatg tacaactcta
    19501 aatgtatctg ccaaatactt ttgataaaat ttcttaaatg tacgcaaagt tggttgctgc
    19561 tcttcaaaaa attgatcccc ataagtaatc aagaaaatat cttcttcgtc ccaatgcagt
    19621 ttgcctgcct ggcctttctt ctgctgataa acactgactt tttgttcaat catagaggcg
    19681 gttttctcag acaaatctga gtcgcgataa atcgcttgta aaagttttgt atacatcgga
    19741 ttcccctttc ttttttggga gcgttcccaa cgatttgatc atattatgga aaacgcttac
    19801 tgtcaatagc atttttttaa ataattttga tatacacaaa aatacaaata agaaagctta
    19861 tgtatcaata attaatagct tcttaaaaaa atatggtctc tcatgatcat gcaagctatt
    19921 cctaaatttt caaaatcata gacaaaaaag aatgtctatt gtactatgga attattctaa
    19981 aacgctccca aaaaggaggt gcatataaaa ttgaaaccta ctatttatga cattgcgaaa
    20041 aaagctaatg tctcaaaatc aactgtctcc agagtattaa ataaccagcc aaatatctct
    20101 gataaagcaa aagcaagtgt acttcaagca atcaaagaat tgaattatga accgagcaaa
    20161 attgcaagag gactgtcagg aaatggtttt gacgcgattc tagcattatc tcatcgtact
    20221 tctcatacga cctatggcaa tccttttttt tcagatgtta ttcaatccat ttctgccgtt
    20281 tctgaacaaa ataacttcga cttgattcta caaacggcaa aaacaccaga agaagagcta
    20341 gaaaaatgcg cgaaaaagat ccaagaaaaa atgatccgag gaattattat tttaagctct
    20401 acgatggatg aaacacttct ttttgaattg gatcaataca atattcctat tgtggtaatc
    20461 ggaaaagtcg gaaacgacta cacgaacatc tactcagtgg acacggataa tttccaggat
    20521 agttacgatt tggtggaaac actgatcaag aatggacacc gtcatatcgg ctgtctccat
    20581 gctccgacag aagtttatgt atccaatgat cgtctcaatg gttaccgtca atgcttattt
    20641 gatcaccacc tagatatccg taatgaatgg attatagacg gcggctttcg cttagaagat
    20701 agcatggcag caatgaatga tttaatgaag ctacgagaaa gaccaactgc gattttttct
    20761 accgatgcat taaaatcatt gagtatttac aaagtggcag aacaatctgg aaagcagata
    20821 cctgatgatc tttcgatcgc aggattcagt gatcccttat tgtcctccct attttcgcca
    20881 cagttgactc ggatagacat tccgacaaat cagttaggaa tcaaagccac agaactttta
    20941 ttccaactaa tccaccacca ataccctgaa gaaaaaagaa tcattctttc aaccgagatt
    21001 actctcacag attcgatcca acaacaagaa atgtagaaca gaattataaa agaacagaaa
    21061 cactgaggat aagtcagtta ctctgttctt ttttcaatca ctcatccctc gttaccccct
    21121 cccggtattt tagcgaattt ttcttatttt ccatctgttt tagatagctg tggataacta
    21181 taatgtttgt gttaaactat tttttccaca gggaagaaaa aactattttt aaccacgttg
    21241 tgtataagtt tttatagttc gtttttttaa acacatttac ccttatttca atccatgtta
    21301 tttggggata cttatctcta aaacataaat agaataacag acatgtctac tggttttgtt
    21361 ttattcgagg aattattcaa taatagctag caaaatatta aaaataaccg ctctatcttc
    21421 ccatcaattt attttgcatt caaaaaatga tctatcttta cttttgttga ttgacttctc
    21481 ttggaagatg atcgttccga taccttaatt ggatacgaac cgatgttgtt aagatccttt
    21541 cccacttttc ttctagttag acaaattatt ttagacaaac caaaaaagag gttgtgacaa
    21601 taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca ttgcgtcttc
    21661 tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg ctacaccgac
    21721 ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga tgatcttacc
    21781 tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga tcgcagcatc
    21841 cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat ttgtttctaa
    21901 tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc ctcctgagac
    21961 tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac ctggtaagat
    22021 atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa ccattacttc
    22081 gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta cttcacgaag
    22141 caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg gatcgattga
    22201 tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt tcatacgtgc
    22261 tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta cttctttggt
    22321 acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg tgatcactgc
    22381 ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac cttgagtgat
    22441 atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag taccaggttc
    22501 accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt cagagccagt
    22561 tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac atgtgaagac
    22621 agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga tgtcttcgga
    22681 aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta cttctttacg
    22741 ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc cctcgcggat
    22801 ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga ttacatcttg
    22861 ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca aggctgtatc
    22921 ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg atagtccttc
    22981 acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg ccatcaaacc
    23041 acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag aatcagacat
    23101 catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga tgctgtcttt
    23161 tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga tcagaccacg
    23221 acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga tttcttgttt
    23281 ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg tcgaatgttt
    23341 gtaccctaag tctttcatac ggtcaagcat cttagaagtt tctgttactt tgaatcgttt
    23401 gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata ctaattcttg
    23461 ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt ccggtgtttg
    23521 gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag gcatgatctc
    23581 gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc attcagtaaa
    23641 tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat gaacatatcc
    23701 gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt cgccttcgcg
    23761 tccttcttct tccattgtca agtagtagtt acctagaacc atatcttgag aaggagtaac
    23821 aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca acatacgtgc
    23881 ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc catcgaaatc
    23941 ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt gtactaaaac
    24001 tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca gcaatactgg
    24061 gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac gttcgatttt
    24121 acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac gcatcacgaa
    24181 tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca tcttcaagaa
    24241 aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta gcaagttttg
    24301 acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg ggcggttacc
    24361 tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta ctgcttcttg
    24421 cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta acaaacgttt
    24481 caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac ttgttgcaaa
    24541 acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag ggataacgtc
    24601 cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa tatccaaacg
    24661 gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt tcaattcggc
    24721 tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg cacccatagc
    24781 agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt ctgtcaacaa
    24841 ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag caaaataaat
    24901 gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg aaggaatccc
    24961 tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca tgcgttcacg
    25021 gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc ctttataacg
    25081 gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa aaatccgctc
    25141 acagaacaac ccttcgcgtt caggttttaa ggtacgatag ttaatcgtct caggtttttt
    25201 tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta tttgcatact
    25261 ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat ggcaaaccgc
    25321 aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta aattatcttc
    25381 tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt cagcagcttt
    25441 cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat caacagtgat
    25501 caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt cgatatccaa
    25561 gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga atgattccgg
    25621 tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt tcacacgtcc
    25681 gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg caccgtaagc
    25741 ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag ctttacctcc
    25801 caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca atttgtcatc
    25861 gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc catcaaatgg
    25921 ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac cggcttcacg
    25981 aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg ctacgtggat
    26041 acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga tgttcatacg
    26101 tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag gtaggaatgg
    26161 catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac gaccggccat
    26221 tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta acatgtttac
    26281 gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt gaacgatacc
    26341 gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt tttcgccaaa
    26401 gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg gcgtgacttt
    26461 ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac ccatttcgtc
    26521 taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt cttcaggtcc
    26581 taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag tgtatacgtc
    26641 atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc cttcccaagt
    26701 catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca tagatggtcc
    26761 gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg gacgttggtt
    26821 gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat ctaatgcacc
    26881 gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac cgtcatgttt
    26941 acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag taccaaccca
    27001 tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac ccatcaaggc
    27061 acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg caactacttg
    27121 tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt tttcactttg
    27181 agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg ggctgtttgc
    27241 ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt ggtcagtaac
    27301 acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga atttatttac
    27361 tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag gagtttcgat
    27421 cggacacata cggccatagt gggagtagtg aacgtcacga acttcataac cggcgcggtc
    27481 acgagtcaat ccgccaggtc ctaaggctga aagacgacgt ttgtgtgtca attcgccaag
    27541 agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt ctttgatact
    27601 tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag tatcttggat
    27661 cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga attggttttg
    27721 taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat catcgacatt
    27781 gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt cggctggacg
    27841 aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt cacgttcagg
    27901 gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga ccacaccgtc
    27961 ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag ctagtgtttc
    28021 catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg tttctggatc
    28081 aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta attttttgtt
    28141 tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga aacgagcatt
    28201 cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac gttcgtaaac
    28261 gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt ctttttcgac
    28321 tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat cggaaccaaa
    28381 tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga tacgtacata
    28441 agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag ggataacagt
    28501 agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga cacctggtga
    28561 acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg ttccttgttc
    28621 tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc cagtctcgcg
    28681 gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat gcgcacgagc
    28741 ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca aagatagatt
    28801 gccattgaag tcatcgattg gcaaaatgtc ttcaaatatt tctctaagtc cctcatctaa
    28861 gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca acacttcact
    28921 gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt gtccagccaa
    28981 gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt tacaataata
    29041 tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg caaaaaaaaa
    29101 ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt gtttccttat
    29161 attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat agtttttgca
    29221 acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt tcttctgctt
    29281 tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga caattcttaa
    29341 ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga ggaacttcta
    29401 tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac tttagctact
    29461 ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt tgtatctatg
    29521 atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct aagtggctga
    29581 gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta ttttccgact
    29641 tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa ttaaggagga
    29701 aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac tagctttagg
    29761 aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa caattggcgt
    29821 attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca aagaaggatt
    29881 agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa atgcccaaaa
    29941 caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc ctgatttgct
    30001 acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca ctatcccgat
    30061 gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca atgaaaaacc
    30121 tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa tcggcttact
    30181 gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg gtgaagcaaa
    30241 ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg tcaaagtcaa
    30301 agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc tagctaaaga
    30361 tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa gtgttgtcgg
    30421 tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc aagtcaaaga
    30481 cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggcaaacaaa cagggatcat
    30541 ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa cagcgaatga
    30601 tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc cagacagcat
    30661 caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc tcttttctta
    30721 tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata cgtcactttc
    30781 cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg tgcagggatc
    30841 gcggcagtaa gtattacaaa tggctatcat ccattgacag cttgtcttct aggcttttta
    30901 gggggtgcgg cagcagggct tgtttccgga ctgcttcaca caaaattgaa aattcccgcc
    30961 cttcttgcag ggatcatcac catgactggt ttatattctg tcacatcccg tgtgatgggt
    31021 gcgccgaaca tctctttatt gggtcagcca acgatctttc attgggtcga gtcattcggc
    31081 ataacaaaag acaacgctgt actgatcgtc ggtctgcttt ttgctttgat cgttgtatgt
    31141 ttacttgttt tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac tggcgataat
    31201 ctggcaatga gcgaagcaaa cgggatcaac acagataata tgaagatcat cggttatatg
    31261 atttctaatg gatgcattgc tctttctggc tccttacttg cgcaaaacaa cggctttgct
    31321 gatttaaatt ctggtattgg aacgatcgta attggacttg cttctattat catcgcagaa
    31381 gttttgttac gtaaccagcc actatttctc cgtctcttga ctgtcgtttt aggtgcggtc
    31441 atttatcgtt ttattttagc gttagtcttc gaactgaatg ttgaaccaag tgattcgaaa
    31501 cttgcttctg ctttcgtgct agtgatctgt ctttctttcc cgcagatcca gcaaaaattc
    31561 catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta acattggaaa
    31621 atctccatca tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg aaaggcatta
    31681 atctttcctt agatcccggc gacttcgtga cgatcatcgg tggaaatggg gctggtaaat
    31741 ctactttgct gaacagcatc gccggtacat tttctgtcga tcagggtcaa attttattga
    31801 atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc agtcgcgtat
    31861 tccaagatcc taaactaggg acagccgttc gtttaacggt agaagaaaat cttgctcttg
    31921 ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa gaccgaacat
    31981 ttttcaaaga ccagctggtt cggctaaatt tggggttgga aaatcgttta tcctcagaaa
    32041 tcggtttgct gtctggagga caacgtcaag ctattacgtt attgatggcc acattacagc
    32101 gtccagaact gatcttatta gatgaacaca ctgcagcgct tgaccctgct acttccaaaa
    32161 cggtgatgga attgacagaa aagctgatca ctgagcagaa tctcactgct tttatggtca
    32221 ctcatgacat ggaagacgcg atccgctatg ggagtcgact gatcatgctg catcaaggaa
    32281 ggatcgtcgt agatatccag caagaggaaa aacagcagct cacagtgccc caattattag
    32341 cgttgttcaa acaaaacagc ggcacggcat taaaagacga tcaagtactt ttatcttcat
    32401 aatcagaaag aggatgggac aaaaaatgaa cgcccaccct cttctttttt ctctttcctt
    32461 ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga aaactatttt gttctctcct
    32521 tcttcctgat agaatggatg ggaaaggagc ttgatgccat gacaacgaaa gacaaggtac
    32581 ttcaattatt gaaaaagaca ccagattttc tttccggaga aaagttggca cagcaactgg
    32641 aagtttcccg gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca ggctatcgtt
    32701 ttgagcacgg gcctacaggg tatcgttacc tgccctctga cgtactagat gcaaaagaaa
    32761 tccagcaaga gctacggtcg tttattcctg atttgaaagt acaaacgatg ttgacttctg
    32821 aatctacgat gaaagatgcc aaactggcag cagctcaagg agattctgtt tcgaagttat
    32881 ttgttgctga tacacaagaa caagctcgtg gacgttttgg acggcctttt ttcgctgaac
    32941 ctggacgagg aatctacatg agtctcttgc tccatccaga caaacggttt gatgaattgc
    33001 ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc cattgaatct gtaaccggtc
    33061 gttctgcttc gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa gtctgtggga
    33121 ttttatctga agcagtcagt gatatggaaa gcggcaggat cagtcatatc atcattggga
    33181 tcgggcttaa tttttctgtt cctcaaaatc attttccagc tgatctccaa gaaaaagcca
    33241 tctctgtctt tcccgacgga caagctagtg tcacaaggaa tcagctaatc agcaaaatct
    33301 ggcaagaatt gttcacttat ctttctcacc cagaaaaagc gttggacttc tataaagaaa
    33361 aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact tatgtcggtc
    33421 ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca aacaaatgaa cagaagatga
    33481 gtttgtcttc tggcgaaatc agtctcacat ctatccgtac gcaataatta tcagatttca
    33541 caaaaggaca cctgatctca tacagtgtcc ttttttctga attctcttca aaagactctc
    33601 cttattctat cagctgacag tttctctatc ctctagaaaa aggtattcta aaaatatcta
    33661 taaagcatgg tacactttct ttaaatgaat caatgcaaaa aacaagagag tcatcagttt
    33721 acacgatcaa aagagatagc aggccgcttc ctttttcaat cttatagata gaccatgggt
    33781 aacgataaaa acaaaaaaga acttgcgact tattttgccg taagttcttt tttagctagt
    33841 gccttaatct cttaatccca acaaacgtct cgcatagatc acctgaccag agtgcatagc
    33901 agcatcatca ataatagaaa cgagacgcat tcctcgtgtg actggtggtg tccagttctc
    33961 gtcaattact tcatctagct cggaaggttc cagccttctt aagtaagaaa tagcaaaatc
    34021 agcagctcct cgcaagtatc ctagcagatc ttctatttga ttagcttgga tctgctttgc
    34081 ttgttccaga ctatgcttcc aatcttgttc gttatctgct atcgtcatcg gaaaccggct
    34141 ttcccagtct tgactatgcc aaattggaga agttcctgct aaatcagcaa tctggaaatc
    34201 taattctcga gcggtatgcc aagccagcca agtcattgat ttgatctgcg gggctctatc
    34261 ttccactgga aaacgattgg cctcctcaat agagacgcct tcaaacaaac gttcaaaccg
    34321 atcgtatgcc cgatccacac tctcgcatag catatctaaa taatccattt attttcctcc
    34381 tttttattta tcctacttac tttttcctaa ttctaggata cctacttata tgttactaca
    34441 cacccttatt ttttacactt taacagtcac catcatcaat agtttggaga ttgaaagaaa
    34501 aagatagtgt cgaactatca attgaacaag aagcaacaat atgaacgttt cattaatatt
    34561 ctttaaataa acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa aagagtataa
    34621 taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa gtttactagt
    34681 cctagttgct ttgttgttca atatcttttc gtatggtgtg gccgtttcag cggaaacata
    34741 cattcctaaa gaccctggga atatttataa tgatctaact ggtaactatg gcttgttagg
    34801 aattgctagt caattccacg tatttgctaa aaataagaca actgtgaatg cacatacaga
    34861 cggaaatata gcaacacatg aacttgatgc aaaaaataat tttggtacag agcttcatga
    34921 aggtttattg aaccaagaaa taaattatat acaaaaaact gattctttcg ctaattcttc
    34981 aggcatacct acaggtagca caagaatgaa taaatttgtc gttggcagta acgtggatgt
    35041 agcacttcaa gatggtcatc ctacagtcaa tggatccagg cttgatcact taactccaga
    35101 tgatttttat aaagatcgtg ctgggcatga atatattgat ttcgagcagg aatttgctaa
    35161 attgaatgta gcagcaaatg atcttgcaac gatcactcca gctaaaacat atactgctgc
    35221 agattttcca gacatgaaca atcggacaat cgatttaact gatttatcag ataccgggtt
    35281 tttattggta aatatcgatg cagaagtgtt aacgatgaat acgccacttc aaatcattaa
    35341 tccaaatgat caagtagttg tatttaatgt catcaacagt tcttctgcat tgaacgtaca
    35401 gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca gaagattttt cggatgcgaa
    35461 tatctcttgg aattttggaa atgagatgac tgatttgaca atcagtgctc cttttcaagg
    35521 aacaatcctt gcgcctaatg ctactatccg cattactcaa aaccaagacg gaacgattat
    35581 aggaacaaat gtgattttag atgcagctac caatcgttgg gatcctaacg aaatatttat
    35641 cagcgacaat ggtacagata cgacagacac aagcgacagt acagatacat ctaccacaag
    35701 tgatagctca gattcatcaa cttcaagcga cagtacagat actacctcta ccacaagtga
    35761 tagcacggat acatcagctt caagcgacag tacagatact acctctacca caagtgatag
    35821 cacggacaca tcagcttcaa gtgacagtac agatactacc tctaccacaa gtgacagcac
    35881 ggacacgtca gcttcaagtg acagtacaga tactacctct accacaagtg acagcacgga
    35941 cacgtcagct tcaagcgaca gtacagatac tacctctacc acaagtgata gcacggacac
    36001 atcagcttca agtgacagta cagatactac ctctaccaca agtgatagca cggacacgtc
    36061 agcttcaagc gacagtacgg atacatctac cacaacaagt gatagcacgg acacgtcagc
    36121 ttcaagtgac agtacagata ctacctctac cacaagtgat agcacggaca cgtcagcttc
    36181 aagtgacagt acagatacat cctctaccac aagtgatagc acagattcat caacttcaag
    36241 cgatagtaca gacacgtcgt ctaccacaag cgatacacgt catgcaagtg tctccttctc
    36301 aaaaacaagt tcggataaca ggacgaactt taacttaact aataaaacaa gcaatgacaa
    36361 tggaaacaaa tcaaaaagag cacttcctaa aactggaagt caatcgaata attggataac
    36421 actcgcaggc gttatattac ttgtaatcgg tttaagaatc acttttatga gttataagaa
    36481 atcgaaaagg taaaaaaata ttcaagataa aaaagagcaa cgtcgctaac ggacgttgct
    36541 ctttattttt gctttttatc ataaatacaa gaactacaat tcaatttgtc aaaaaacacg
    36601 tagatttcct tctgagaaat ttatttacgt atcgaacctt tccctattca gtctttgcga
    36661 tccaaaatca gttgggtaag ggaaaagaga ttctcttttg ttcgaagaga ggtggcaggt
    36721 aatttcttta tttccttcaa agccttttct gtgtaagagg ccgctaactt cctagcttct
    36781 tccacgcctc ctgtatgttc aacgattttg cgtacggcgt cgacatcttc ttctgtcatt
    36841 gccgctttct tctctaaata aggtagtagc tttggtttgt ttttttgcaa agagtagatc
    36901 aacggtaaag aatagacccc ttgacgcatg tcttctagta caggtttgcc gatttcctgg
    36961 ctctcttttg tataatctaa aatatcatcg ataatctgaa aggccatccc gatagaaagt
    37021 ccgatatctc ctgctttttt tgcgaagcgc tcgcttgttc cactttcaaa agcgccaaca
    37081 gaacaactta attggaacag ttcagctgtc ttacctgaaa tattttccaa atattctttt
    37141 acagaaagtt cataattata gcgatcatcc atctgcccta attcgccatt caatactttt
    37201 tccatactgc gagaattcaa ttggagactt ttcaaggaag tggagtaatc agacaatagt
    37261 ttaaaacagc agacaaataa atagtctcct gcatagacag aaacgacatt accaaactga
    37321 ctacggatat taggctgacc acgtcgcaga tccgcttcat caacgatatc atcatggatc
    37381 agtgtggccg tatggagcat ttcgatagaa gctgccaaag cgatggcttt gtctcgatct
    37441 tgctctggtc caaattgtga gaacaatagc tgataggcag gacgcaacag ctttccgccg
    37501 gcatggatcg tttctttgat ggcttgttcg acaggtttgt ttttcaggcg gacagtttct
    37561 tccatccgtt ttagtgtttc tgtcaattct ttacttaaag caggatagtt ttcccacatt
    37621 ggatgtagct tcataaaaaa actccttgtg tgacgattat tttttggtaa acattgggct
    37681 ccatttttgt ttggttccct gctcataatc tttttggaat cgttgaagtg tctgcacatg
    37741 tcgtaaaaga taattcgctg cgatcccgat tgcaatccct gataaaatcc ctaaaactga
    37801 aaggactggc aagtacaaca tgactgtcca tgattgtgca atgaaagaag ccatcaaaag
    37861 ctgtccgaca ttatgcatga aaccgccggc cgcactaatt ccgatgatac tgacacgttt
    37921 tggtcctaat tgcttgacca ataacattcc aaaataacta agcaaagcac cactcatact
    37981 ataaaggaat gtggaaatcg ttccgcctag caaagtagtc aggaatagtc gtagccaaag
    38041 tagtaagaag ctgtcttgtt ttttcatggt aaaaatggca acgatcgtga ttagattggc
    38101 taagcctagt tttgcaccag gcgcaaaaga aaaaggataa gggatcatat tttccaataa
    38161 accaatgatc actccttgtg caacaagtag cgaaatataa atcatttttt gtaatttact
    38221 catggtgtgt gaaggtctaa taaatcagac tgtcttcgtc acctccatct gttgaacgga
    38281 cttcgatcac taatttatgt ggcaaacaga cgatggtttc gccatttttc gttgcccagc
    38341 ctcgacgtac gcagatctgg tcatcgcaat ctgcttcttt tatccgtact cgatcgccat
    38401 ctatctcgat caagttataa tctccgtgtt catctttgta agtatatgtg ttgctctctt
    38461 ctccagccat caaagggaat gttttgattt ctgtgccatc cacacgcagt accgcttctt
    38521 tttctgctgt tgcggtgttt tgctgaagac caaaaatcat gactggtaaa aaagaaagaa
    38581 cagtcaaaag caaaataata aaaacatccc aaggtttgag cagacttttt tcagcaaatt
    38641 ctttaaggct catttttgtt ctcctttttc cagattaact cacccaattt tagcataaaa
    38701 acaagaggtt gtgacacctg tgtcttgctt tgagtaataa gaacgaagaa tagaaaacag
    38761 ctttccatgt tttctaattc tttgggctta ttaccgagaa gcagcaggtg gaacaccgtt
    38821 tataacaaga ggttgtgaca gaagtgggca acttcaagaa ataagaagga atttacgaaa
    38881 attacttttc aagtttttgt gaaatttcgg cttatttttc gaagaagcta cttctggaac
    38941 accgtttata acaagaggtt gtaacagagg ttgtgacacc tgtgtcttgc tcatggaggc
    39001 ttatctcggg catcttattc ctttaatttt acataaaaag gaggctgtga cggtttgtct
    39061 cagccccttt ctattgaaat cttgcattca aataacgttc tttatctatt tatttcttgc
    39121 cgtactggga acttggtcta ccgtaccacc aattacctag tgtacgttgt agccatggaa
    39181 ttacccagcg atccaaccca atcgcacgtc cggaaccgtt catcaatgcg aaagcaacaa
    39241 aaatgaacca aatgtttacc cagtaaaaca tgcctgataa gcagaacgtg atcaccaata
    39301 caatcgttgt cgcactgcat agccatgtga ataatccagc aatcaatgct aatgccaagg
    39361 caatttcaaa gaataccatg aatttttgga agaacattgc tacatcattg tttggcatga
    39421 tcacttgcat gatctttgtg aaccagtctg gtgacttctc aatcacagcc attggttctt
    39481 caccgtatgc ataactcaaa ccaaaatgtg cagcttgagt cgctgcttct ccggcacttg
    39541 cccctgctcc gctggcagct gtggttgcat caccagcagc agaagcacca ctggcaacat
    39601 ctgttacatt gtattcccat gtccaaggga acacactgtt tccagtgaac catgatcctt
    39661 caccaaacca agtacttggt ttgaacactt caccattccc aatgattttt ttggaagctt
    39721 caacgaacca aactaaacca tagaaaatac gaagtggcac actccataag acattgccgt
    39781 aacgagaaga atgtcctcga gtcacgttgc gatcatcttt gatatggaaa aactcatgca
    39841 ttaaatattg gaacatataa taaccagaac gaatatcaaa gaagtatttc aaattcacga
    39901 tatgtttcat tacgatagct aaaaatccgc ttaaatggat cttatcaaat aggttagcta
    39961 ccccccattt ggcaccaata gaaaccataa atccttgata gtttccttta aatttatgct
    40021 tttctccgcc tttgatcgaa gccacgatat tcgctgcggc tgtgtgtcct gtttgttcag
    40081 cagcttgaac gatctgcggt gtcggtgttc ctgggaattc ttcataaaag actaaatcac
    40141 caatgatata gatgttttta tcttcgtagc cttttgcctg catatattca ttggcgatca
    40201 accgctgtcc gcgtgctgat tctaatccaa agtcagcagc gtcagaagtt cctttgacac
    40261 cagctgtcca aatcagtgtg tgagtcggaa tgttgcgtcc gtctttcaat ttgatatgat
    40321 cagcagccac ttctacgatt ggtgcattca ataataattc cacgttgtgc ttacgcaaat
    40381 agcgttcagc ttttgcagca tcatttcgat ccaacatatt caagatcgtc ggcattgcct
    40441 ctacgaccat cagtgtgatt tcgtttggat ctaatttata atctttggct aggcgatttt
    40501 tccagtcgat cagttcccca atcatttcaa taccggtaaa tccagaacca cagacaacga
    40561 aagtcagcat ggctttacgc agttcatcat ctggttccaa tgcagctttt gctactgtct
    40621 tctcaatatg ttcccgaatc cttacagaat cttcaaatga ccaaagggtg aatccgtgtg
    40681 ctttgacacc aggtgtacca aagtcattcg gctctccgcc cattccaagg atcagctgat
    40741 caaaaggata ggaacctaat ttggtcttga cgatttttcg ttctttatcg ataccgatta
    40801 cagtgtctgt gacaagttgt acattctttt gacgagcaaa caaatgctgc agatcgtatt
    40861 ggattgcttc tggttctaca cgtccgccag ctacttcatg caactcggtc atcatagtat
    40921 ggtacgaatg acgatcgatc agagtaattg ctacatcttc ttttttcagt ttctttgcca
    40981 agtattttgt agctgaaaca ccggcatatc ctgctcctac aacgacaata ttttgctttg
    41041 ccattgatca tccgctcctt atagactatc tatataatga atatatgata attcttttag
    41101 tttgatactt attacacaat ctccatatat tatatagatg ttctgttctt ttgtctaact
    41161 ccttttggat ttttttcaaa ttttaaaata aagcgttttt tcacgaaatt tgctgaaaga
    41221 aaacgtttga aatattacat gggttattta tcaaaaatag ttgacttata ggctaaaaag
    41281 attagaatgt ataatgaatc acaaagaaaa attgtggact agaaatggaa tggtggggta
    41341 ttaaaatgaa gaaaaaacgt tttgtaactg gatttgccgt attagcattt tcagctctag
    41401 tgttaggcgc ttgcggcgca gatgacaacg gaagctcaaa ctcttcttct gaatcatcta
    41461 cagcacaatc ttcgactgca aaaagcacaa cagaatcatc tgcaaaagtt gtagctggcg
    41521 gagatttgca agacggcaca tataaattag aagaaaaaaa ttattcaaat ggttaccgtg
    41581 cagtctttga aatggttgta aaagacggca aaatcactga atcaaaatat gataacgtca
    41641 atgaaaacgg cgaatcaaaa acaaaagatg cagaatacaa caaaaacatg gaagcaaaat
    41701 ctggcacaaa cccagaaaaa tacattcctg agttgaatga ccaattctta aaagcacaaa
    41761 gcgcaagcgg tgtggaagtc gtaactggag ctactcactc atctgaaagc ttccaaaact
    41821 acgcacaaca attgatccaa gctgctcaag ctggtaacac agatacaatc gaaatcgaca
    41881 atggtgctga tttgaaagac ggcacttaca agcttgaaga aaagaactac tctaacggct
    41941 accgtgtgca attcgaaatg acagttgctg gcggcaaagt aactgaatca aactttgatt
    42001 atatcgacaa agacggtaaa tcaaaacaag atgatacaga atataatgaa aatatgaaag
    42061 caaaaagcgg tacagaacca aaaacttaca tccctactct gaacgacgaa ttcgtcaaag
    42121 caatgggcga agaagatggc tcacctgcta atgtagaagt tgttacaggt gctacacaca
    42181 gctcacattc attcatcatg tacgcacaac aattagtgaa tgcagctgaa aaaggcgaca
    42241 ctcaaacaat cgaagtagat aatatcgtaa cagaaaaata atcacataat catactaata
    42301 aaagttggag ggtgggacga tagccagatt aggcagtcgt ctcacctttc aaaatgaaga
    42361 gaggaaaaag atgcggaaaa aactactatt ttccttaatc acactgggca tctcttttat
    42421 ggtgctaact ggctgcagca actcagcaaa taaaacaacc actgaaagtt cagcagctac
    42481 caagatattg aaagatccat attctgatga acagttcttg ttagggactt acgttcgtat
    42541 ccgtgtgtat gatgaaggca aagaatcagc attgaaacct gccttcgatc gagtaaaaga
    42601 acttggtgat aaaatcacga tcaatcaaaa aggttctgag atcgatgaag tgaatgagca
    42661 agctgggatc aaaccagtaa aagtttctga tgacgtctac acgttagtca agcgggccta
    42721 cgaatatagc caagattctc aaggcggatt cgatatggcg attggtgcaa tcacacaatt
    42781 atggcgaatc ggttttgatg acgccagaaa accttcccaa gaagaaatcg atcaggcatt
    42841 gaaattagtg gattatcata agatcgaact aaatgacaaa gaaaaaactg tttatctaaa
    42901 agaaaaagga atgatcatcg atttaggtgc aatcgccaaa ggttacatta cggacgaagt
    42961 agtgaaagtc ttgaaaaaac aaggagtgac cactgccatc gttgacttgg gcggtaatgt
    43021 ctatgtctta ggccacagcc caagaggcga aaaccaagac tggacagtcg gcatccaaga
    43081 tccgaacatg gctcgcggat ctgttttagg ttctattaaa gaacggaaca aaacattagt
    43141 tacctctgga atttacgagc gttatttaga agtagatggc aaaaaatacc atcatttgtt
    43201 tgatccaaaa acgggttatc cgttcgacaa tgatatcgca agtgtcacga tcataacaga
    43261 taaatcaatt gatggtgacg ggttgtctac agcagtcttt tcaatgggtg tgaaaaaagg
    43321 actggaatac gtcgaaagcg aattgaataa cgggacggaa gcgatcttcg tgacaaaaga
    43381 cgataaagta tatgtgaccg atccaatcaa agacaccttc aaattatcgg aggactccca
    43441 ttacacgatg ggtgaccgtt ctgaactaaa ataaggagta cgaaccatga cactaaaaat
    43501 ctttttagaa gtagttgaaa tacgaacaaa ggtggctagt gtatttcctt ttgcggtagg
    43561 ggtattattc tctatcgctt tttttcatga gatacattgg ctaaacactt ttttattttt
    43621 tattggaatg ctagtttttg atttagcgac taccgctatc aataattaca tggattatga
    43681 aaaggctcat tctgaagtat ataagtatga agaaaatgtc attggccgca gaaatctatc
    43741 tcctgtattg attcgaaaca tgattttagc tatgattgca tttgtttttg ttatcggcgt
    43801 tattctaacg atcaatacgg gttggctttt acttttgatg ggatttatct gttgttttat
    43861 tggagtattc tatacattcg gaccgattcc tttatctcga atgcctttag gggaaatttt
    43921 cagtggggtg actatgggcc ttggaatttt tgccatggtc gtttatgtca atacattcgg
    43981 ttcagatgtc tatgatctga cagtcaactt ttcatctgga caatttgatt tggtaggaaa
    44041 tctttgggct atagcagcaa tcgtattagc ttccttaccc ttggtgttta cgattgccaa
    44101 tatcatgttg gctaataatt tgcgggatct tgaccgtgat attgaaaatc atcgctatac
    44161 actagttttt tatatcggac gtcctgcggg aacagtctta ttccagctat tgatgtatgc
    44221 ttgctatttc gtgattttaa tggggttgtt gagcggtatc tatgaatggc cgatcttggt
    44281 caccttcttg accttgccaa ttgtgaagaa gcatttggat caattcaagc aaacgttacc
    44341 ccagcctatc agtttcagct attccatcaa aaatatggtc ttattcaatg ccagttatgt
    44401 attggggcta ttactaacaa tcattatgca agccatttaa cagcatgaag aggttgtgaa
    44461 aaaactgccc tgcatcaagt aataagaaca gccaaataaa aatagctcct tatatttttc
    44521 gtttggttgg gcttattacc gcagatgcaa gcttttgaac accgtttatc aggaggttgt
    44581 gaaaaagtcg cttagtttcg agtattaaga aaaattttgt gaaaatggct ttccacattt
    44641 ttgcataaat ttggcttaat accgagaaac tggcttttga acaccgttta tcagaggttg
    44701 taaaatcagt gttttgtcct gagtattaga gaaacaaatg gaaaaatagt tcctcatatt
    44761 ttgccgtttt ttgagctaat accgaaggac agctgattga acactatttg ttcggaaaaa
    44821 gtgactaact atgacacaat ttttttcaca gtcccttttt tcacttgatt tgattcgtac
    44881 cagcgaactt gacgagaact ttcttggttt ggtaactata tggtattctt ttaacatcct
    44941 cgtttgcttc ttctaattta tttatacaaa gtcacacttt tttaaacatc tgttcttcga
    45001 tacatttcta tttcttttaa acatcgatac caatattttg aacttaagag tgcataccaa
    45061 atggattcat ttactcttcg tcagttacga agataatcaa aatctccaac ttccctactt
    45121 atttcgtttt gagaattatc gaagcaatct tacataaata actaaaaaac aaaaaaccag
    45181 aaaatcagta tactgatttt ctggttttac atggtttgtt caataaaaag ctgttggaac
    45241 tgttaatatt aaattatcgc atcgttacga attcttccgc tcctgttgga tggatggcta
    45301 ctgtattgtc aaagtcttct ttagttgcac ccatcttgat agccacggca aaaccttgca
    45361 gcatctcatc gacaccaaca ccaatagcat gtaagccgac gattctttct tcttttccga
    45421 cacagatcaa tttcatttcg catttttggc gataatcatt taatgcaaag tacattgggg
    45481 taaagcgtga acgataaact ttcaattcat tttcgccata tttttccaat gcttcttctt
    45541 ctgtcaaacc aattgttgca attggtggat gcgtaaagac gactgtcgga acaagtttat
    45601 agtcaagata agaatctttc tttccattaa ataaacgttc tgataaacgt ctaccagctg
    45661 cgattgcaac aggagtcaga tctaatttac cgatcacatc accaacagca aaaatatgtt
    45721 catccgttgt attttggtat ttatctactt tgataaaccc ttttttgtcc aattccacat
    45781 ttgtattttc cagacctaat ttttcggtat tcggtacacg tccaccggca aaaagtacac
    45841 aatcaccagt taaggtttca ccattttcaa atagcaccgt atattcgtca ttgtttttag
    45901 taatttcttt agcaatatga tgtgcgtaag tttttattcc agcttcttgg tacatttgga
    45961 tcaaattgtc cgaaagcatt ttatcaaacg ttctaagtgg tcgttctttt cgatatgccc
    46021 acatgacttc actacctaaa ccacgaaata cacctgaaag ttcagccgca atgtatccag
    46081 cacctaaaac gattgctctc ttaggtaact ctgttaattc aaaaaatcca tttgaatcaa
    46141 ttgcgtattc tccaccagga atggtcattt tacttgcacg gcctccagta gcgatcaaga
    46201 ttttaggagc acgataggtt tcaccgttta cttcgatcgt ttgactatcg ataaaagtag
    46261 cgtaaccttt tatagcctcg acatgattgc tatctaatcc tctttgataa gcaccgtgta
    46321 gaaaatcaat atatttttct cgtctttcta ctagaccggc gaaatcaaca ttttttaaat
    46381 ctgcttggat accgtaactt gaggcatctc gttcaattgt ttccaaaatc gtacttgctt
    46441 gccacataac ttttttaggt acacaaccga cgttgacaca tgttccgccc aattcattcg
    46501 cttcaatcag caaaactttt gcgccatgca tccctgctct gtttgctgaa gcaataccgc
    46561 cagagccgcc accaattacg atgtaatcat atatttgcat tttgttcctc cttattgaag
    46621 agatcaatca aaacctttat ttaacgatcc tttttctaca gaaaacaagt gtaacacttt
    46681 tcttgaattt tgtatgcgaa gatgcttaaa acagggatat atcagctttt tccttattta
    46741 cgttttcgga tcatatctcc aggtttcacc ggctgtgcga ctggcatagt taaaatcatc
    46801 attggatttg gtgctcggtc aatggattcg ccttcttcat tatgcataac ttcaacggtt
    46861 tgactgaaat gactgaatcc tggaccataa aattcgattt catctcctac agaaaacaag
    46921 tttctctgac gtatcgtagc aatctgtgtt gcctcatcat aagataatac ttcaccgaca
    46981 aatttatata ctgggatttt acgacgttca ccgaataatt gttcgttttc agttggtgta
    47041 ttgtagtaaa aacctgtagc taattctcgt tgtgctactt tccataactc gtcgatccat
    47101 tcttgcttgc agacatagtt ttcaggatct tccatataac tgtctacagc cgctttatag
    47161 acatttgaca ccgtagatac ataatggatc gatttcattc ttccttcgat cttgaagctg
    47221 tctacgccgt tttcaatcaa ttctggaata tgttcgatca ttgacatatc cactgcactc
    47281 atcgagaatt cttcttccac ttcgcctgta tcagttaaac tgtttcgttc ttgaccaaaa
    47341 ggcatatcga acaatccata cttccagcga caagattgag aacatccgcc acggtttgca
    47401 tcccgcatcg acatatggtt ggacaatgtg catcgaccag aataagagat acacatggca
    47461 ccatggataa atgcttcgat ttcggtatcc gtatttttac gaatctctgc cacttcctcc
    47521 attgaaactt cacgagctaa aacgacacgt tccaaccctt cttctttcca gaattccaat
    47581 gtctcaaaat tagttgctga agcttgagtg gataaatgga tcggcagacc tggggcttct
    47641 gtcgcacaaa tttcgatcaa agctggatca gaaacaatca ctgcagaaat accgacatca
    47701 cgcaaactgc ggaaaaattc ccctgctcca tcttcatttc cttcatgggt gaccatatta
    47761 gcagcaacgt ataccttcgc atcatgttct tttgcaaaag caactccttc agccatttct
    47821 tcataagtaa aattacctgc acggcttctt aaaccatatg cattcccgcc aatatatact
    47881 gcatctgctc cataatggat cgcggttttt aacttttcca gtgttcctgc tggcgctagg
    47941 atttctggtc gtttcaattt tctttttact gtcatttctt tcatcatact ccttatttga
    48001 tagcagaagg atcaatataa aagaatcctg tatctaattc acggttttca ggatgcaatt
    48061 tactgatttc gctagtcatt ttttccgctt tttcttgatc ccagctacct tctaaaattt
    48121 gtttttttgc ttcggcgaat agtcgagtga tgtttacgaa tttttcgcct ggagtaaata
    48181 atccgtccaa tttccatgta cagtaatgat gctgataaag ttcattcaat tcattcgaga
    48241 gattcagatc attgctggca aaaatatgtg tgccatgact atcttcataa atagaataat
    48301 gtgtctcttc ttttttaggt tccgagataa aaagacctct ttcacgatct ttttgttcgt
    48361 cttgtttcgt atagttatag taattctgta gcagtggacg tttggattga tggatgcatg
    48421 tagcaccata cacaagggtt tctacaggaa tatcgagttt ttcttccatc gccttcatct
    48481 cttcaaaagg aacttcacga gcaagtactg ctccaactgc gccttttttc gcccagaaat
    48541 tgatttgacg tgcgctcgtt actaatgttt cagcatcata aatatacggt atacgcaatt
    48601 caggattttt tttcatcgta taaataatac ctgtatcacc aactgtgatc acatctacgt
    48661 ttatttcttt taaaaaagcc aaatattcag gtacattttt cattttttca ggatgaatga
    48721 tcccatttac agcaatattc gcttttttac catacttgtg tgctaattct accaattgtc
    48781 gctgttcttc tcgtgtaaat gaagcaggca gacgcaatcc aaattgctct tcaccgaaat
    48841 atagtgtatc tgcacctgca gcaagcaact gttctgcttg ttcaatagtt tctactgtgg
    48901 ttatgagttc gatcataatt ttctcctcct acaacttcat aatagtagca tagctctaaa
    48961 aactttccaa tacttcttct atttaaagtc ggatttttga aaattatttc acaaaaaatc
    49021 aaaataaagt tcgatatatt ctgtccaaat ataaaaagag attgtgacaa aaatcttgtc
    49081 acaacctctt aatcaagtct tactattata aaaattcagc tacacaggcc aagtaactat
    49141 agggaaccta ctgtttcctt tttaggtccg cgaacattca atgtgatctt accttttgaa
    49201 gcacctactg ttacgtggtc acctaaacga atttctccgc caagcaaagc ctcgctcaat
    49261 cgatcttcaa tttctttttg aagtgcgcga cggattggac gtgcaccata ttcaggatca
    49321 aatccagctt tcccgatcac atcgattgca gatggtgtga ttttcacttg aatatcttgt
    49381 tctttcaatc gtttgataat cgctttgctc atgatttgga cgattgtatg gatttcatct
    49441 ttactcaatg aatggaagac gacagtctca tcgattcgat tcaagaattc aggtcggaaa
    49501 gctttcttta actcttctaa gatacgtttt tgcatggctt tgtgatcttt agtaacatct
    49561 tggacattga atccgacatt tttttcttct cgaatttgtg tcgctccgat atttgaagtc
    49621 ataatcatga ttgtatttct aaagtccact tttctacctt tagaatctgt taaatgacca
    49681 tcatccaata cttgcagaag taaattgaaa acgtctggat gtgctttctc cacttcatct
    49741 aagagaatca ctgaatatgg tttttgacga attttttctg tcaactgtcc accttcatca
    49801 taccctacgt atcctggagg agacccgatt aatcggcttg tactgtattt ttccataaat
    49861 tctgacatat caacacggat cagggcatct tcacttccaa acatgacttc tgaaagtgct
    49921 ttggccaatt ctgtttttcc gacaccagtt ggtccaagga acatgaatga accaattgga
    49981 cgatcaggat ctttcaatcc gcttcgtgca cggcggatcg cacgtgaaac tgctttgaca
    50041 gcctcatctt gaccaacgac acgttcatgc agaagtcctt ccagttctaa caagcgttcg
    50101 ctttcttttt tctccaactg ctgtagaggg acaccagtcc actccgatac aactgtagca
    50161 acatcttctt ctgttacacg atcagaataa ccttgctcct gttttgcttc aattgcagaa
    50221 actttttgca gctttttacg tagtgctttt tcttgtctgc gtaattgagc agcattttca
    50281 aagtcttgtt tttggatcgc tgcttccttt tcttgtacta gttcatcgat ttctagtttg
    50341 atcacagttg attttgttaa gtgatcggtt tgatccaaac ggactttcgc tgctgattca
    50401 tcgatcaaat caattgcttt atcgggtagt tgacgagaag tgatataacg aacagaaaga
    50461 ttgactgctg ctcgtacagc ttcatctgtg atctccactc catggtgctc ttcatatctt
    50521 gtacgtaatc cttgaagaat cacttctgct tcttcaggag ttggctcatc tacttgaatg
    50581 cgagcaaaac gtcgctctaa tgcagaatct ttttcgatat atttttgata ttcatctagg
    50641 gtcgttgctc cgatcgtttg taattctcct cgagctaacg ccggtttcaa gatattcgaa
    50701 gcatcaattg ctccttctgc tccacctgca ccaattaagg tatgcaattc atcaatgaat
    50761 aagattactt gaccatcatt atagatttcg tcgatgacct ttttcattcg atcttcaaat
    50821 tcaccgcgat atttcgtacc agcaaccaaa gatcccatat caagcatcat caggcgcttt
    50881 tcttgcatgt cttctggcac ttcacctaaa atgatttttt gagctaaccc ttcagcaatg
    50941 gctgttttgc ctacaccagg ttcacccacc agtacagggt tattctttgt ccgtcggcta
    51001 agaatctgga taagccgttt cacttctctc gaacgaccaa caactgggtc cagcttgttt
    51061 tcttttgcta atttcgtcaa atcacgtgct aatgaatcta gcgtaggtgt tccttgcggt
    51121 gtttgccgtt tatcttgtac atttacattt ctacgacgat tcattccttt tccagcagaa
    51181 tcaccagcta gccccatttt ttgtttcaga acttgacggg cttttgaaag gctgatacct
    51241 aagttgatca tgattcgagt agccatgata ctttcatctt ttaataagct catcaaaata
    51301 tgttctgttc caactttttg agaaccagtt ttcttcactt cgctgctagc taaagcaaaa
    51361 acttgtttca ttcttggtga atatggaaga aagactcctt tagggtaaga acggatacca
    51421 ccataaccaa tcagatgttc aatttcttca taaatttccg tttcattcaa atccatttca
    51481 cgtaatactt ttccagcaat tccattttgc tcgaccacta aggctagtaa aacatgttct
    51541 gaactaactg attgatgttt caaccgtttt gcttcttctt gtgcaatttc taatactgct
    51601 tgggctcttt cagtaaaaag ttcttccatc taatctcact tcccatcttc ataacttaaa
    51661 cgttccagaa atgctcggag aatccgagca cgtgtatgat cttcatattc attgaaattc
    51721 aatgtgtttt ttgcaattgc actcaacatc aagtttcctt ctttttttgt aattatttgg
    51781 tcttcataaa gtttttgaat aatagcaaaa gcatttttct cactgattgt atcatcaaat
    51841 aactgattta tctgctcaag taattgtttt ttatctgaaa tccttacttt tgcaattcgt
    51901 atataaccac caccaccacg tttgctttct actagataac cacgttggat ggtaaaacgg
    51961 gtattaatca catagttgat ttgtgaagga acacagttaa agaggtctgc catttcaatc
    52021 cttcgaatct cgatcatttc actattttcc agaattttct ttatatatga ttcaattaaa
    52081 tccgaggtat tctgatgtgc catcttattc attccctttg actaatattg accttaatta
    52141 taacgaattt gataatagaa agaaagaaga acgctttttg ttaaataaat cctttagaat
    52201 caacaaaaaa aaaatacgga atatactatt ccgtatcgac gcgcccaaga ggagtcgaac
    52261 ccctaacctt ttgatccgta gtcaaacact ctatccagtt gagctatggg cgcatattca
    52321 atttaaaaaa attataaatc atttgaaaac aaaaagcaag ataaaaaaaa tcgggaagac
    52381 aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc tgagctactt
    52441 cccgtttaaa aaataaaaaa gac
//
LOCUS       DAFEOM010000012        48211 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.012, whole genome shotgun sequence.
ACCESSION   DAFEOM010000012
VERSION     DAFEOM010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.012"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(27..1523)
                     /gene="lysS"
                     /locus_tag="LP524_000852"
     CDS             complement(27..1523)
                     /gene="lysS"
                     /locus_tag="LP524_000852"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBL2233923.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="LP524_000853"
     CDS             complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="LP524_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBL2233924.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="LP524_000854"
     CDS             complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="LP524_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBL2233925.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="LP524_000855"
     CDS             complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="LP524_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBL2233926.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="LP524_000856"
     CDS             complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="LP524_000856"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBL2233927.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="LP524_000857"
     CDS             complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="LP524_000857"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBL2233928.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8054..8524)
                     /locus_tag="LP524_000858"
     CDS             complement(8054..8524)
                     /locus_tag="LP524_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HBL2233929.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8570..8995)
                     /locus_tag="LP524_000859"
     CDS             complement(8570..8995)
                     /locus_tag="LP524_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBL2233930.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9062..9331)
                     /locus_tag="LP524_000860"
     CDS             complement(9062..9331)
                     /locus_tag="LP524_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBL2233931.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9342..10937)
                     /locus_tag="LP524_000861"
     CDS             complement(9342..10937)
                     /locus_tag="LP524_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL2233932.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="LP524_000862"
     CDS             complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="LP524_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBL2233933.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14490..15050)
                     /locus_tag="LP524_000863"
     CDS             complement(14490..15050)
                     /locus_tag="LP524_000863"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBL2233934.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15403..16377
                     /locus_tag="LP524_000864"
     CDS             15403..16377
                     /locus_tag="LP524_000864"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBL2233935.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16547..17947
                     /locus_tag="LP524_000865"
     CDS             16547..17947
                     /locus_tag="LP524_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBL2233936.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18025..18747)
                     /locus_tag="LP524_000866"
     CDS             complement(18025..18747)
                     /locus_tag="LP524_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL2233937.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18826..19332)
                     /locus_tag="LP524_000867"
     CDS             complement(18826..19332)
                     /locus_tag="LP524_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBL2233938.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19455..21254
                     /locus_tag="LP524_000868"
     CDS             19455..21254
                     /locus_tag="LP524_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBL2233939.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21322..22896)
                     /locus_tag="LP524_000869"
     CDS             complement(21322..22896)
                     /locus_tag="LP524_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBL2233940.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23151..23660)
                     /gene="mreD"
                     /locus_tag="LP524_000870"
     CDS             complement(23151..23660)
                     /gene="mreD"
                     /locus_tag="LP524_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBL2233941.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23664..24518)
                     /gene="mreC"
                     /locus_tag="LP524_000871"
     CDS             complement(23664..24518)
                     /gene="mreC"
                     /locus_tag="LP524_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBL2233942.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24673..25311)
                     /locus_tag="LP524_000872"
     CDS             complement(24673..25311)
                     /locus_tag="LP524_000872"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBL2233943.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25312..25962)
                     /locus_tag="LP524_000873"
     CDS             complement(25312..25962)
                     /locus_tag="LP524_000873"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBL2233944.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25975..26874)
                     /gene="rsgA"
                     /locus_tag="LP524_000874"
     CDS             complement(25975..26874)
                     /gene="rsgA"
                     /locus_tag="LP524_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBL2233945.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27039..29108)
                     /gene="pknB"
                     /locus_tag="LP524_000875"
     CDS             complement(27039..29108)
                     /gene="pknB"
                     /locus_tag="LP524_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBL2233946.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29105..29845)
                     /locus_tag="LP524_000876"
     CDS             complement(29105..29845)
                     /locus_tag="LP524_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBL2233947.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29858..31216)
                     /gene="rsmB"
                     /locus_tag="LP524_000877"
     CDS             complement(29858..31216)
                     /gene="rsmB"
                     /locus_tag="LP524_000877"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBL2233948.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31216..32154)
                     /locus_tag="LP524_000878"
     CDS             complement(31216..32154)
                     /locus_tag="LP524_000878"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBL2233949.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32147..32638)
                     /gene="def"
                     /locus_tag="LP524_000879"
     CDS             complement(32147..32638)
                     /gene="def"
                     /locus_tag="LP524_000879"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBL2233950.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32653..35061)
                     /gene="priA"
                     /locus_tag="LP524_000880"
     CDS             complement(32653..35061)
                     /gene="priA"
                     /locus_tag="LP524_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBL2233951.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35314..35619)
                     /locus_tag="LP524_000881"
     CDS             complement(35314..35619)
                     /locus_tag="LP524_000881"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBL2233952.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35624..36238)
                     /gene="gmk"
                     /locus_tag="LP524_000882"
     CDS             complement(35624..36238)
                     /gene="gmk"
                     /locus_tag="LP524_000882"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBL2233953.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36725..37009)
                     /locus_tag="LP524_000883"
     CDS             complement(36725..37009)
                     /locus_tag="LP524_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233954.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37027..37917)
                     /locus_tag="LP524_000884"
     CDS             complement(37027..37917)
                     /locus_tag="LP524_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBL2233955.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37937..38155)
                     /locus_tag="LP524_000885"
     CDS             complement(37937..38155)
                     /locus_tag="LP524_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HBL2233956.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38125..39825)
                     /locus_tag="LP524_000886"
     CDS             complement(38125..39825)
                     /locus_tag="LP524_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBL2233957.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40091..40864
                     /locus_tag="LP524_000887"
     CDS             40091..40864
                     /locus_tag="LP524_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233958.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="LP524_000888"
     CDS             complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="LP524_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HBL2233959.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNSWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41580..41682)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41688..42659)
                     /locus_tag="LP524_000889"
     CDS             complement(41688..42659)
                     /locus_tag="LP524_000889"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBL2233960.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="LP524_000890"
     CDS             complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="LP524_000890"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBL2233961.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44629..45447)
                     /gene="purR"
                     /locus_tag="LP524_000891"
     CDS             complement(44629..45447)
                     /gene="purR"
                     /locus_tag="LP524_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBL2233962.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(45614..46438)
                     /locus_tag="LP524_000892"
     CDS             complement(45614..46438)
                     /locus_tag="LP524_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBL2233963.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46411..47091)
                     /locus_tag="LP524_000893"
     CDS             complement(46411..47091)
                     /locus_tag="LP524_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBL2233964.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47114..48052)
                     /locus_tag="LP524_000894"
     CDS             complement(47114..48052)
                     /locus_tag="LP524_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HBL2233965.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
BASE COUNT        13201 a        10455 c         8697 g        15858 t
ORIGIN      
        1 gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga
       61 attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca
      121 gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg
      181 tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga
      241 tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga
      301 tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt
      361 ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg
      421 ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct
      481 aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca
      541 tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg
      601 atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc
      661 attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta
      721 tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac tttttccatt
      781 ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg
      841 tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc
      901 aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg
      961 ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag
     1021 tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga
     1081 cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct
     1141 gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact
     1201 tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga
     1261 tcttgtagat gcgcaaatcc agttttccct tttcctcggc gagtcatcat acgaccagct
     1321 acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca
     1381 tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct
     1441 tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact
     1501 tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat
     1561 aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg
     1621 aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt
     1681 gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt
     1741 tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag
     1801 ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt
     1861 tttcggctgg tgttggttca ggaatcagtt ctcctgtttc gagataatgc tgtgtgcggt
     1921 gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat
     1981 agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggga
     2041 tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct
     2101 gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg
     2161 cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg
     2221 ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa
     2281 gccattttgc tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat
     2341 ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt
     2401 tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct
     2461 ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta
     2521 agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg
     2581 gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt
     2641 aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt
     2701 tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga
     2761 tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt
     2821 tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca
     2881 atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta
     2941 attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga
     3001 atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg
     3061 aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa
     3121 caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca
     3181 ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac
     3241 tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca tctactacaa
     3301 ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg
     3361 tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc
     3421 aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc
     3481 ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc
     3541 tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg
     3601 gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac
     3661 aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc
     3721 agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt
     3781 gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt
     3841 caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg
     3901 cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag
     3961 gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca
     4021 aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt
     4081 tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt
     4141 ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat
     4201 attgaacagg acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag
     4261 ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt
     4321 cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca
     4381 agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga
     4441 tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag
     4501 cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg
     4561 gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat
     4621 ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa
     4681 aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc
     4741 gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct
     4801 ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg
     4861 cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt
     4921 tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta
     4981 ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca
     5041 aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga
     5101 tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg
     5161 ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc
     5221 ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac
     5281 ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt
     5341 taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca
     5401 tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata
     5461 taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg
     5521 ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg
     5581 tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt
     5641 attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc
     5701 ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat
     5761 taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg
     5821 catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta
     5881 atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca
     5941 gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata
     6001 tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac
     6061 ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt
     6121 gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct
     6181 gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt
     6241 gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa
     6301 atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc
     6361 ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc
     6421 tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt
     6481 ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag
     6541 tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta
     6601 tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact
     6661 ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg
     6721 tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga
     6781 gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga
     6841 ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg
     6901 tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct
     6961 cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac
     7021 tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct
     7081 ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt
     7141 tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc
     7201 atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt
     7261 ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt
     7321 ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct
     7381 gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta
     7441 gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt
     7501 ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat
     7561 tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct
     7621 atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca
     7681 caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg
     7741 tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa
     7801 accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat
     7861 ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct
     7921 ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt
     7981 ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat
     8041 tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc
     8101 gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga
     8161 agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat
     8221 ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct tctccagatt
     8281 cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg
     8341 aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat
     8401 agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa
     8461 acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg
     8521 acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag
     8581 ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata
     8641 taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta
     8701 catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga
     8761 cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca
     8821 tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg
     8881 cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact
     8941 ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga
     9001 ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa
     9061 tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat
     9121 cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa
     9181 actggacttt caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt
     9241 tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc
     9301 gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta
     9361 tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga
     9421 cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg
     9481 acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa
     9541 tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc
     9601 cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc
     9661 acacctaatc ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga
     9721 aaagaagtag tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga
     9781 aacgagttct tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg
     9841 gaaaaaacaa ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg
     9901 atataaggga gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat
     9961 ctaagataga ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga
    10021 gtcaacgcag gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact
    10081 aaaggctgac ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg
    10141 taaacttcca aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat
    10201 ccactataaa tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac
    10261 ggctgtttga acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca
    10321 tagtagtgaa ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc
    10381 gtccctgttt ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc
    10441 acaaaaacac gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat
    10501 cttccttgga aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa
    10561 actacttgga tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta
    10621 aatgcaaaga caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt
    10681 tgcaaagctt gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac
    10741 tgcggaagac cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga
    10801 taaacataaa aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt
    10861 aatacttttg ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc
    10921 atttctttat gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca
    10981 acgctttgac aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga
    11041 tcgtcaagcg tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg
    11101 ctgtcagtgc ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag
    11161 taaagactat atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca
    11221 ttttgatctg cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt
    11281 cgagcagatc atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt
    11341 tatagatctc gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga
    11401 caccgaggtc gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag
    11461 aaacagcttc gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat
    11521 gctgctgagc acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct
    11581 tgaatccaga tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt
    11641 cgttaaggat cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc
    11701 ctacccgccc tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga
    11761 acaacgtatt tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa
    11821 catcatattg accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt
    11881 gcccatgagc ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt
    11941 gttcgatcgt ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc
    12001 gatggattgc ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat
    12061 aacgattcgc aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat
    12121 gcaaagttct cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact
    12181 gtttcaatcg ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac
    12241 ccaaatcgct aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa
    12301 cttgacctgt acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac
    12361 ttaatagccc gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct
    12421 gctgtgccaa gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt
    12481 taaaggccgc tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc
    12541 gatccattgg tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt
    12601 catcagtttc agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc
    12661 caaaagcata tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga
    12721 tatcctcgat ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct
    12781 tattgatgcg aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag
    12841 taacaggaat aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa
    12901 cgccatctac ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga
    12961 caacgtaatc tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg
    13021 tttgacgtct cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta
    13081 ctattttttc ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct
    13141 gtcctctgat caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca
    13201 tttgttctgc tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt
    13261 gcttgcgcca acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca
    13321 tcgtccgata ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga
    13381 agagtgagaa aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg
    13441 aaaatgtctg ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg
    13501 cttccctttc gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta
    13561 aactatttga agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt
    13621 agaattttgc ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt
    13681 aatcttgcaa aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca
    13741 ttttttgcat ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg
    13801 ttggggaaac ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat
    13861 accgcataga atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta
    13921 acgggtaaat atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct
    13981 cttctcgtac atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat
    14041 caccaatatg ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt
    14101 gcaaagatgc gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg
    14161 ttgcaactct ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt
    14221 catccactgg aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta
    14281 actgattcgc ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa
    14341 acgctcgagc aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag
    14401 gcattttttc ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt
    14461 gaataatatc cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac
    14521 gaatgccctt ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc
    14581 aaaggctgat tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct
    14641 tctggcctgc cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata
    14701 atacttttga taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc
    14761 actgccagat ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa
    14821 gtcattagtg ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa
    14881 aggatctttt ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg
    14941 aaagtagctt gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt
    15001 gtatattggt atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc
    15061 cacctaattc taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg
    15121 agcttttttc ttttttatac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc
    15181 taaaagagaa tcgttaataa atgataaaga aattaaaaat ttcttcaaag tttaatgaaa
    15241 taattgatat ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc
    15301 ttgttatctg ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca
    15361 taagcatggg aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa
    15421 aaaaaagatc atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc
    15481 tttgctctag ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct
    15541 aaaactgaag gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa
    15601 atctattctg ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc
    15661 gcaccacaaa aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac
    15721 cgcgatgttg tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct
    15781 aacccagtag atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc
    15841 gtaatcggtt caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta
    15901 gttgacgttg atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa
    15961 ttcccagttt ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac
    16021 aacccagaaa tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct
    16081 tacacaatta tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt
    16141 atcactcgtg ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat
    16201 ggcgaatatg gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt
    16261 atccaaaaag taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct
    16321 gctaagcaat taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct
    16381 ttgaacatga cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat
    16441 ggtttctaaa ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta
    16501 tcatgagacc gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc
    16561 gcatcgaaaa aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct
    16621 gatcagcttt tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga
    16681 aataaatgga ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga
    16741 agcttattct gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc
    16801 aaaatccgag ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga
    16861 acaaaacgga tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga
    16921 tcctgtcagt caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct
    16981 gactgatttg aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga
    17041 ctctttcgtt atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa
    17101 acaattaaaa agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga
    17161 aaaaccaaaa gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc
    17221 tatcgcaaat gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag
    17281 tgatattatg agttggctga cctacaatga tggaaaagtc gatttagata cagaacaagt
    17341 acgtcaatat gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt
    17401 taaaagtaca aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca
    17461 aacagacagt gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg
    17521 ttcacccatt gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat
    17581 cgaagttgat ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga
    17641 aacagatatc gtttccggaa agccaactac acctacacct gctggagtct tctatgtttg
    17701 gaataaagaa gaagatgcaa ctttaaaagg aacaaatgac gatggaacac cttacgagag
    17761 tccggtaaac tactggatgc cgattgactg gacaggtgtt ggtatccacg attcagactg
    17821 gcaacctgaa tacggtggag acttatggaa aacccgcggt tcacacggct gcatcaacac
    17881 cccaccaagt gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc ctgtattggt
    17941 cttctaacaa aaataatata taaaaagaaa gcacccgtac gattggtaca ggtgctttct
    18001 ttttattatc taacaatgtg ctacttacat ttcagcagca gtaaagactt ctgaaacatc
    18061 atcatcgtct tctagcttat ctactagacg ttccaattgc tctttttgtt cctcatttaa
    18121 atcaatcgtc gtttgtggaa tcatcgtcaa ttctgcttgc gccaaagtga actctttttc
    18181 caactcatct cgtacagcag caaaatcttc tggtgcagta taaatctcga atacttcagg
    18241 agaagtttgc agatcttctg cccccgcctc taatacatcc tcaaacatcg tctcttcatc
    18301 aacagtcaac ccatcgcgtt caatagcgat atatcctttg cggtcaaaca agtaactgac
    18361 tgaacccgtt tctcccagtg atccaccgtt tcgagtaaaa gctactcgga catttgtcgc
    18421 tgtccgatta cggttatctg tcaatgcatg gactaagatt cctactccac ccggaccata
    18481 tccttcatac gtgatttcat cgtaattcgt ttcatctacg gcactcgtag cctttttaat
    18541 cgcccgttcc acattgtcgt taggcatatt tgcagctttt gctttgtcca caacaagccg
    18601 caaagctgga ttcatagatg gatcaggacc gcctgcttta gctgccatat aaatttcacg
    18661 agaaagtttc tgaaagactt tccctcgttt tgcatcttgg gcattttttc ttccttggat
    18721 attatgccat ttactatgtc ctgacatgat ttttcctccc ttgttctttt gagaatacac
    18781 ttctattcta gtactatttc acaaaaaaac aaagccacag ccaatctata aacgcttctt
    18841 gaatgccgca aaaagcacac ataatatcac agcagtcaga gcaccgatcg aatccaacat
    18901 gacatcttgg aacataggtg ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc
    18961 tgcgtaccct gttgctgaaa gccaagcaag gatcgctgtc agccaccaaa tttttagctt
    19021 ggggaaaaca cctaaaaaca agctgcctcc caaaataaaa taggtaccga aatgtgcgcc
    19081 cttccttaaa aagaactcta aaaatttggc gtagcccaga tggtcgatac tcaccatact
    19141 tccaccataa ttaaaacgta tacccgctaa tgtgtcttta aatggctgat taggcagcca
    19201 gctttctaat cgcgaaactt gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa
    19261 gattataacg actatcagtg cgattactaa ataaatattt ccgtttttca tttgtttttt
    19321 tgaagaattc ataagtcacc tctgtatttt atcttagact ttttttgaag aaacaacaat
    19381 cattttcata gagacttctt ccataaaaaa gtgctatact taattttgta ttacaatatg
    19441 aagggagttt tttcatgagt tattcattaa attggaatct agattctatt ttttccggag
    19501 gcagtcattc tgatgctttg aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt
    19561 attatcaccg cgtaacaaaa tggtcaccta gttctgacaa tgcagagcag ttaaacgcta
    19621 ttcttcaatt gcaagaaacg attacgaatg gttttaccca atgcagcagc tatattactg
    19681 cattgctttc tgccaatgtc aatgattcag atgcaaaggt cttatcaggc aagctttacg
    19741 caatgctgcc tcgtctgcaa tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat
    19801 ctgacaatga ttggaatcag atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt
    19861 taaacgagat tcgtcgtgat ggctcacaat tattatcaga agctgaagaa aacattataa
    19921 atacgctttc tttagatggt ctaaatgctt ggagcagtca ttatgatacg attgtagcca
    19981 gcatttcgat tcccttcgaa caagatggac aagtagtcga attatctgct ggacaagcct
    20041 tcaataagat gatgggcgat ccagatccaa aagtcagaga aacacttttc gcagcatggg
    20101 aagaagcctg gaaagaaaag gcacccctct tcacggatac tttgaatcat ttagatggtt
    20161 ttagactttc tgactatgaa cttcatggtg tcacagattt tctacaaaaa ccattagagt
    20221 acaatcggtt gaagaaagaa acattgtctg tcatgtggga tacaatccag aaaaacaaac
    20281 aaccccttgt taattacttg actagaaaag ctaatctttt cggtaaagaa aaaatggaat
    20341 ggcaagatca agatgcacct attattttag gcgatttgaa agaaaaaacc tttagttttg
    20401 acgaagcagc agcatttatt atcgaaaact ttaacaaatt cagtccaaaa atggctaaat
    20461 ttgcccaatc tgcttttgaa aagagctgga tcgaagcaga agatcgtcct gggaaacgtc
    20521 ctggtggtta ttgtactgag ttaccagaaa caaaagaatc aaggatcttt atgacctaca
    20581 gtaattctgt taatgaggtt gcgacacttg ctcatgaact aggacatgct ttccatagca
    20641 gcacgatgtg ggatttacct tctttaaatc gcgaatatgc catgaatgtc gcagaaacag
    20701 caagtacatt cgcagaactg atcgtagccg atgctacatt aaaagtcgca aaaacaaaag
    20761 aagaaaaaat caatttgcta gatacaaaac tgcaaaatgc tttagctatg ttcatgaata
    20821 tccattcccg attcattttt gaaaatcgat tctatgaagc acggcaagaa ggattagtct
    20881 cagaagataa gatcacagag atgatggttg aggcgcaaaa agaaggctat caaggagctt
    20941 tagctactta ccatccatat ttctgggctg caaaattgca tttctttatt gatgacgtac
    21001 cgttttataa cttcccatac acatttggct acctctttag tttgggcatc tatgcttacg
    21061 ccaaccaaaa gggagcaaat tttgaagaag aatatatcca tttactacgc gatactgcct
    21121 caatgacgac ggaagatctt gcgaagaaac atttgaatgt cgatcttacc caaccagatt
    21181 tttggcaagc aggtatcgac atggtgactg aagatatcca aacgttcatg gaattgacag
    21241 aagaatttat ctgatttcta ttttatcaca ataaaaaaag agttagtcca gaatgggcta
    21301 actctttttg attaatcatt cttacatgct gacagcaaag tcaggggcaa accattgaac
    21361 tgaaccaact tttacgcttt cgcctggttg aggagcatgg atatattgtc ctccgcctaa
    21421 agcaatcgct acgtggtaag ttccgccttg tgacccccag aataacaagt ctccagcttt
    21481 tgcttgagaa actgaaattt ttgttccagc tgattcttga ggaactgtcc aaccaccgat
    21541 gtcacgacca gttacttgca tgtaaacata gcgtgtgaac cctgagcagt caaagccact
    21601 tggatcttta ccgccccaaa catatggagt accaatatat ttgtaagctt ctgctacgat
    21661 tgctgcacca ttcacacttc cagaaggagc tggagcaggt gttacaggtt ttggttgttc
    21721 aggtgttggt gttggcgttg gagccggagt actacttcct gtgccgtttc ctgtatcaac
    21781 actttgatct gttgatggtg ttgtaggcgc aggtgttgtt gattcttctg ttgttgaaga
    21841 ttcaggcact gttgttgatt cttctgttgc tgaagattca ggcactgttg ttgattcttc
    21901 tgttgctgaa gattcaggcg ctgttgttga ttcttctgtt gctgaagatt caggcgctgt
    21961 tgattcttct gttgaagatt caggtgttgt tgattcttct gttgctgaag attgtgtagc
    22021 tgatgatgat tcttcagtca ttgaagattg tgtagctgat gattcttctg ttgctgagct
    22081 ttgtgcagta gatgaagctt gagtagcttg tgcttcagcc tctgcttgtt cgcgtgcttc
    22141 tttttcagct ttttcttgtg cagcttgttg acgagcttgt tctgctagac gttgttgttc
    22201 acgaatacga gcttgctctg cttcagcttc tgctttttga cggttcaagt cagctttctt
    22261 gtcttcagca gttgcttgtt cagcagctaa tgaagttttc agtacgttca aatcagcttg
    22321 tttagataaa agatcgcctt tttgagattc caaagcagct tggttttcag ctaattcttt
    22381 tagtttagca tcgttttcag cttttttgtc ttctacagct tttttatctt gtttttgttg
    22441 ttccatcaag tcgttgtttg cttttaccat cgttgtcatt gcttgaacgc gtccaatagc
    22501 atctgctaat gaatcagcat tcaatacagc atcgatgtag tttgagcttg tgttgcttac
    22561 ttgtgcttcg cgtgcttgtt tttggattgt atcttcacgt ttttcaatac gttcttgtaa
    22621 atctgcaatg tcttttacta attgtgcaga ttcttgacgc aatgtatctt gttttgctaa
    22681 taggtcttgt gcttgtgtgt tgatttcaga aacttggctt tctaaggcat cgatttgtga
    22741 ttgtgcatct gcttgttgat ttttcaagtc tgcaatcttt tgatcttgct gttgtatctg
    22801 agaatcaaaa tcgtccgccg cggcagcgat cggtgatgct acggcagtca aggtcattga
    22861 acataccatt actgctgata ttaaactctt tttcactctt cattcctccg actggcttaa
    22921 tttaataaat atctaaatat atttaagtct ttcttaacga tataaaaatt gtaccatagc
    22981 agtgtgtcaa cgatataata gttatgttac aaaaatgttt caaaacaact tttaaaaatg
    23041 acaaaactgt tcgtttcaga aaatgatcat gaaaaagccg cgttaagtct agcatttctt
    23101 aagataacaa cgattctttt tcgctttcga cctaaaaata aaaaaagaaa ttattcatca
    23161 ctgaataatt tcttaaaggg aaaaatgaat aatacaaata taatcatgtt caacagcagt
    23221 gtaggtccta aaaaccttgt aataaaatac gtgttattta ctactgctaa tttaaaaatc
    23281 aactggacca ccatcgtaaa caattcataa cccgtgacaa agatgatcat actgaaaaat
    23341 tcagtaaaga tgttgacatg gataacgtct ttcattacat acatcaacca tacaattaaa
    23401 gggagagcta ctgcatagat accgataacg ccaatatagt aagcgtcata gatagccccg
    23461 agaacaatcg tggtaatcag taaataacgt ttttcaaacg ccatactgca acataataat
    23521 gccaatatca gaaagtgggc atttgacata taggtgcctt tcgaccactc ccctagcatt
    23581 cttgttaaat gaccatctat caacatcaaa aagaacaaaa cgattggcag atagtacttc
    23641 attgtttctt tcttcaacat ggactaatcc tccgctagcc gttggacaat cgtcacaact
    23701 gacacgtcat acatttctgc ataaggtttc acataaactt cccgatccag cccataacta
    23761 tctggtttcg ttttaataac cgtaccgatc ggcaagttag ctggagaatt ccctccaaga
    23821 ccggatgtct ggacaacatc cccttctttg atatccgtat caccagttaa ttgggtcacc
    23881 actaaggcat ggagcttttc atcatagttt ttaagcaaac caaacgcttc gccattagcc
    23941 gaagataccc gtactggaaa atgattggag ctttcattag aggatgacag taattcgatt
    24001 ttagacgaag ccgtattgac ctcgatcact cggccaatca atcctttttg cgacatgact
    24061 gccatatttg cttcgatacc gtctttagaa cccttgtcta cgatcagcat gtcctgccaa
    24121 ctgtcaggcg aacgagtaat gacatttgcc gttacttttt cgtaactagt cagcgtctga
    24181 ttcaactcaa gttctttttt caacgcttcg atttctctct cgtaattctt gttttgctgt
    24241 gaaacgtcat catagctatc gattttttct tttagccgct cattttcggc ataagttgaa
    24301 aaaagattat ggacagaatc tactccattt cgtacccacc gtactggggc agagattgtt
    24361 ttatcaacca ttgcaacact gtcattaacg atggattgga agaaattggg ctttccttca
    24421 ttcgctcgct gcgcagcggt cacacttagt atcgtcacca cgatgataac aataaccaag
    24481 gtaataataa tatttttatt aggattgaac ttcttcacaa agacacctcg aattaattag
    24541 actcttgtgt tcaattttat cactaaatgc cgtgaaacaa aaggtaagtt gctcctctta
    24601 agcttttttt tagcaaataa tagattctgt catttttagc ctgaaagaca aattttttta
    24661 tttgtttatg gatcaatctt tgctttgaat aactgcaatc ataccatctg aaaaagaaaa
    24721 ttctgcggta tctgaaagaa attcgttact tgcataagat gtcggatatt ctacttcttt
    24781 attttccaat acatacttgc tgccttttaa tgtaagattc gagacgggag tcaagcagca
    24841 ataagctaaa tacttcatat ctggtatctg tcttactata tgctctccag gcaaataata
    24901 agacaaataa ttttgacaat ctctgataat aagctgatgg gcaaagggtt tgaagcgagg
    24961 ttccatacct aaccagagat tggctaaaag atgatccaat cttccgcccg tagctccaat
    25021 caaagtgact tccgcttgcg ggtaacgttc aaatgttttt aagataccta actgtgtatc
    25081 tgtatcgtct ttttctgcag gggaacgggt atactctttg gcttttgcaa atacttcctt
    25141 ctgctcttcg ttcgacaatg aatcaaaatc acccactgcc agatctactg ggtagcctgc
    25201 ttgaagcagg tacagcgcac cacggtctat cccgacatag gcatcgtatt tggacagcag
    25261 agactgcggc cagttttgcg gattacctcc agcagctaat aaaattttca tttagtctaa
    25321 tgcctctttt aatcgttcga tttgtccgac tggattttcg gctccataga tatatgatcc
    25381 tgcaacgaag acatccgccc ctgcatcttt acagattttt gctgtttctg gaacgattcc
    25441 gccatctact tcgattgcat aagtccaatt gttctctcta cgtagatcag cgagttccgt
    25501 aattttttct actgtttctt caataaagct ttgtccgccg aacccaggat tgacagtcat
    25561 gactagtact tgatcagcca aaggcaaaac atgtttgatc atggagactg gtgttcctgg
    25621 attgataact actccagctt taactcctaa gctttttgtc atttgaagtg cccgatgaat
    25681 atgaggagtt gcttcgacgt ggacagtaat gatatctgct ccagcttttg caaatgctgg
    25741 aatgtagttt tctggatcaa cgatcatcag atggacgtct aacggtaatt tagtaacagg
    25801 acggattgcc tgaacaatat ttggccctaa tgtgatattt gggacaaatt ggccatccat
    25861 tacatctacg tggatgtaat ctgcgcctcc tttttccact aactcaatgt ctctttgtaa
    25921 attggcaaaa tctgcactca aaatggatgg tgctatcttc attcttgttt cctcctattt
    25981 tcgtttacct tctttttttg catacattgg ttttcttttt tcgatttctt gtaagaattg
    26041 caaataattg tcgtaacgtg tctgtgcgat accacctgtt tctactctat gttttacttc
    26101 gcagccaggt tctttagcat gcatgcactc tctaaatttg caatggacag aagcttctac
    26161 aaattcagga aattgtctcg gcagttctcc tgcttcaatt gtcaaaaagt caatcgagct
    26221 aaatcctggt gtatctgcta ctaacccgcc atagatagga atcagttcca catgacgtgt
    26281 tgtatgccgt cctcttccaa gtgcatcaga aatctctcct gttgctaaat tcagttccgg
    26341 tgatatttga ttcagtagcg tcgattttcc tgctccagac tgacccataa agaccgatag
    26401 ccgttctggg aagtaacgga tcagttctgt cgtatcaccc gtatgattgg gtacaatcac
    26461 cggatagcct attttagtat aggtttgctt gatctcttcg attttttctg ggtcgtctga
    26521 caagtcggtc tttgtcaaaa aaataatcgg ttcgatcgac tcgtattcca aggtaactag
    26581 aaagcggtca agtagattat aagaaaatgc aggctctacg atacttgtga caatgattcc
    26641 aagatcaaca tttgtgactg gtgggcgaac taattgattt tttctcggca agatttccag
    26701 aagatagccg tctgtttgat tttcactctc aaagatcacc tgatctccga caagcggcgt
    26761 gattttccga ttccggaaat ttcctcttcc tcttgtttga taggtctctc cgtttgcgta
    26821 tacatagtaa aaaccgctga ttgcttttct gatttgtcct ttgataagtg ccacctccgt
    26881 ttttgtttat tttaccatac gaagaggaaa gtaggtgtta ttccatgcaa acttctcaaa
    26941 gtatatggct tgaaaattga tcaaaagaga tagaaacaca aaaaagagaa tgcaacaaat
    27001 caatgtcgca ttcccttctg atatatttta ttcagcactt attttgaact ttctgaggtg
    27061 ctagctgatg aacttgtgga gctggaagat tcttcttttg aagagttgct tgtgctactg
    27121 gagctacttg agctgctaga actgtctgaa gagtcttttt cagaactaga agacgctgga
    27181 tctggtcctt tagagtaaat tacgttcaca accattcctt tggtaattgt cgttcctgcc
    27241 cctggtactg tacgaatcac tttatcttta tccaccgtgt ttgaatattc ggaagtctca
    27301 tgcccgatat attctgcacc gacactagct aaataacttt gtgcttcgct ttttgtatag
    27361 ccggagatat cattcaattt aggctctgtt ccttggctta cgacaaaatt gatgtaagtt
    27421 tcttctgcta caacttcacc cgaggctgga tcctgactga tgatcgaacc tgatggtaca
    27481 gtgtcgctat agtcataagt agcactaata ttattgatat tgaatccttg tgtaacaagc
    27541 caattacgaa cagtttcgta gttttctcct acatgactac tcaaagtcac tgtttctggt
    27601 cctttgctga ctgtgaaagt aatcgtgtct gttttaggat cgacttctgt atcttctgat
    27661 ggagattgcg aaatgatcat tccgctttct acctctgaac tgaattcctc ttctttttga
    27721 atcaagtcag cagaaaaccc taattctttc aaacttgctt cggcatcttc ataactttta
    27781 ccactgtaat catctaattt gatttttttc gtgccgctac taatatacag agtgacttcg
    27841 cgtttttgct tgactgtcgt tcccgctgcc gggtctgtct tcactacatt accttcttcg
    27901 attttgtcat ctgcaatttc ttctgttttt gttgctactt ctaatttcgc ttttgcgagt
    27961 gtttctctag cagaagcttc agaaagatca gaaacatcag gaacaacaac ttctcctcgc
    28021 ccgccagaag caaagaatac agctgttccg attcctaaaa cagcaagaat agctaatagg
    28081 atcagccacc attttttctg tcctttcttt ttaggatcag gcagaacttc ttcctcctct
    28141 ggtaaggttt cgttttctgg cttgtctttt ggcagaggca tagatttgaa tgaatcaggc
    28201 attggtgcat cttccgtaag gggtgtgata acttttgttt cattatccat cacatgaggc
    28261 tgccattttt cttcgtttgc acgagctggt gataatactg tagaaagatc atcagacatt
    28321 tcctcggctg acttataacg gtctgcaggt tctttagccg ttgcccgtaa aatcacgttt
    28381 tccagtgctt gtggcacatt tggatcaagt gcttttatag atggaagatc atcttgaaaa
    28441 tgttttaaag caattgttac agcagattca ccatcaaacg gtacgctacc cgtcaacatc
    28501 tcataaagga tgatccctaa agcataaata tctgactgct tcgttgccat acttcctctg
    28561 gcctgctcag gggacagata atgaacagat cctagcatcg tattcgtctg cgtgatcgat
    28621 gtttcagata aagcgatagc aatcccgaaa tccgtgattt ttacaacacc ttcattatcg
    28681 atcagtacgt tttggggttt caaatctcgg tggatgattt gatggttatg agctaaagaa
    28741 atagcagaaa ggatctgctg cataatattt actgctgttt cataaggaac aggataatgt
    28801 gtttggatat atcgtttcaa gtccattcct ttgacgtatt ccatcaccag atactgcatg
    28861 ttgtcttctt caccaacatc gtaaacacta acaatgtttg gatgaacgag ttctgttgct
    28921 gctaaagctt ctctctgaaa tcgtcgaatc gcagtctggt cattttgaaa gtcaaatcgt
    28981 aagactttaa ctgctacatc acgatccaag attaaatcgt gcgccaaaaa tacatttgcc
    29041 ataccaccgc taccgatatt tccagtgatt tgataacggc catttaattt tttgcccaac
    29101 tcgatcatac actgtcctcc ttatattcga taagaagaac ggtgatatta tccgctccgc
    29161 ctgcttcatt ggcacgatca atcagctcag ttactttatg agacaatggg ccttcttgat
    29221 tgatgatcgt tcctatttct tcttcagaca gcatgttcgt caagccatct gaacataaca
    29281 gcagtcgatc ttttagttgc caaagatgag tagaaacatc tacttcgact gttccaggca
    29341 tgccgacaga acgtgttaaa atatttttcc ttggatggtt cactgccatt tcttcgctga
    29401 tttctcctga tttgactagt tcattgacta aagaatgatc ctccgtcaat tgcatgattt
    29461 tatcttcacg aatcaaataa gctctgctat ccccaacatt ggcaatcgtg aaccagtcat
    29521 ctgacagagc ggccgctacg attgttgttc ccataccgtt atattccggc tgttcttgtc
    29581 cacgctgata aatcagcata ttttcttctt gaatcgattg aatgaaccat tgggcgattt
    29641 tttcatcgtc aacagcttct tgtttttccc aagcttcgcc tagattcgtt accgccatct
    29701 gactggcaat gtctccggca cgatgaccac ccatcccgtc tgctaataca gcaagcttga
    29761 gacctgaacg gctggtaaat acactggcat agtcttgatt tgtgttacgt cgtcttccta
    29821 catctgattg atattcaatc tgcatttttt cacctcatta ctttttgcgc aaacaacaaa
    29881 tgaaaaatcc atctgtgtaa aactgatgcg gatacaaagt caacattttc tcttcgattg
    29941 atgattgtag cataggattg gtcaaaacat caatcctctc aaaatcaggg tgtctttcca
    30001 agaaagcaga aacaacgtct tggttttctt cttttaagat cgtacaagtg ctataagtca
    30061 gaataccgga agactttaaa gttggtgcgc agctttctaa aatttccaat tggatttttg
    30121 gtaaattcgc taattcatcc ggctgttttt tataacgaat atctggtttt cgccgaagca
    30181 gcccaagacc tgaacaaggc gcgtctacta aaatccgatc aaactgctct gcttcgaact
    30241 gtttactgac ttctctagca tccatctttt gagcgtagac agcagaagag acacctagac
    30301 gtgctgcatt atcttcaatg agtttgactt tgtgttcgtg tacatctaaa gcgaccactt
    30361 ctccaccgca tgacggatct aaaaaagaag caatatgcgt cgtcttacct ccgggtgctg
    30421 cacaggcatc taagacatga tgttcaggct cgatttgcat agacggcgct acaagcatcg
    30481 aactttcatc ttggatcgtc atgacgccat cgtggaacaa tgagctagaa gctaaatgcc
    30541 cttttttcgc tactataccg tatggactga cttggctttt ttcagctgtg atctcttctt
    30601 cttgcaaaac agtgatggcc tgatctctgg aaagtcggcg tgtatctact cgggcactag
    30661 catggctagg ctgatatagc gataaaccga gcttttcagt ttcttcgtat ccgatttgat
    30721 ccaccagtcg tttagtcatc catagaggca ggctgatttt gacagaaagt ctttcgatag
    30781 gatcagcaat gccttgcaga tctggtgctc cttgacgctg ataattcctc aagacaccat
    30841 ttacaaattt ccctgtccca ggatttcctt ttacttttgc gatttcgacc gcctcgttta
    30901 aaatagcatg tgcagggact ttatctaaaa attctaattg gtatacagaa agataaagaa
    30961 gcgtcttgac ccagtcatcc actttttttg ctttagcgat caatggtgca acgtaaaatt
    31021 ccaatagtcg ctgatggcta atcgtaccat agatcaattc tgtaaataaa cccacatctt
    31081 tttgatttag ctcgctttgt ttgatcatct cattcaacaa aagattcgag taggcgccac
    31141 ccttgttcac tcgctctaat gtcagtaatg ccatatatcg tactgatttt ttcatttttt
    31201 tagggatttt tttcattatt tcacctgctg accaatctga acttgctgac cgcttccgtt
    31261 aaggaaatca tgtacggcct gttttccttt accagctggc tgtaattcat tgatggctaa
    31321 gacggtctgt ttaccgcaag caatccatag attttttttg tctttccgga tgatcgtacc
    31381 aggtgctttg tctgtagttt ctgccaatac ttcaaccgat aataatttcc agcgtatgcc
    31441 ctcataggtc gtgaatgcaa ttggccaagg acgcattccg cgtacttggt tgtcaatttc
    31501 ttctgcagtt ttatgccaat cgattgcttc ttgttcccgt gtgatgtttg gcgaaaaagt
    31561 tgcttctgac tcgtcttgag gtactggttt ttgtccatcc aaaatattgg gcaatgtttc
    31621 tagcaatagc tttcttccta aaaggctaag cttttcaaac atcgtcccta catcatcttg
    31681 tttagtgatt ggtatgcttt cttgcgcata gattccgcct gcatccattt ttttgatcat
    31741 ttccatgatc gttacaccag tttctttctc cccattcatg atggcataat gaacaggcgc
    31801 acctccccga tatttaggaa gtaaagaagc atggacattg attgccccta attttggtac
    31861 ttgcaataac ttttcaggca agaattggcc aaatgccgca gtgacgatca catcaggcac
    31921 caattcaatg atttcttcca tttctggcga accggagatt ttctctggct gcagcacacg
    31981 gataccgtgc ttcagagccg cttcttttac cggtgtcggc gtaattactc gtttccgccc
    32041 aactggacgg tcgggctgtg ttaccactgc ctggatctcg taacctgatt ctatcagccc
    32101 ttcaagaatc ggcacagaaa aagcaggtgt gcccataaat actagcttag tcatcaagat
    32161 gttcctccat atattcttca agttcttctt ccgggatctg ctgtatcatt ttctcaatga
    32221 acaagatacc gtctaagtga tcgatttcat gctggaaggc acgggctaaa tagccaaaag
    32281 ccgtcacttc tatctcttct ccgtctcgat cataataacg cacggtcact tcatctgctc
    32341 gcttgaccgt tccatagaca tgcggaatac tgagacaacc ttctacgtca aggctttcac
    32401 cttttgcttc gatgatttct gggttaatca gttcaaattt ctcgccttca tctacttcga
    32461 tcacagcaat tctcttgttt tgtcctactt gaggagcagc gatccctatt ccgtcattcg
    32521 caatcattgt ctcatataaa ttgtctaata aagcgattgt ctcgtccgtg attacatcga
    32581 ttggctgagc agttcttttt aatttatcat tagggtggat caatatagga tagcgcataa
    32641 tttggccctt tcttaaataa agtttaatgg ttcgttgtca attgccacaa aaagaccttg
    32701 tcgctgttct ttctggcttc cattcaaaat ttcatccaaa acatgaggaa gattcggttc
    32761 atgtttatat ttgatgatta gttgataata atatcggttt ttcactctca aaatcatgct
    32821 gggcgttggc cctaatagca agctttgagg gcttaacact tcttttagct gattggcaat
    32881 ctgaaagatt ttctttgccg ctacttgttc ttcaggatgg ctggcggtta ttttcacagt
    32941 aaagtaataa ggaggatagc ctccacgatg ccgtacatgc atttcctgct gataaaactg
    33001 ctcataattt tgttttttag ccaattctat ggcgtaatgt tgaggattga atgtttggat
    33061 cacgacttcg cctgcttttt ccgctcgccc tgctcgtcct gaaacttggg taagcaattg
    33121 gaatgtatgc tcactagaac gaaaatccgg taaattcagg gaggtatcag cattcaacac
    33181 cccaactaat gtcacttctg gaaagtccaa acctttagca atcatttgtg tccctaataa
    33241 tatatctgct tcttttgcac cgaatttttg taaaatctgt tcgtgtgccc cttttcttcg
    33301 agtggtatct acatccatac gcaaaatcct agcttgcgga taaagttctc ggagttcctc
    33361 ttctactttt tgagtacctg ttccatagta gcggatttta ttgccgccac aattaggaca
    33421 gcgattggga ataggttctt catgtccgca ataatggcag cgcattgaac gtgtatccat
    33481 atgcagtgta agagagatat cgcaattagg acatggaaga acaaatccac agtccctaca
    33541 catcacaaaa gacgaatatc ctcgacgatt gagcagtagg acagtttgtt cttttttgtc
    33601 taatcgattt tgtatttttt cctgcaaatt agcagaaaaa gtagaggtcc gatgattctg
    33661 aaactcttca cgcatatcta cgatctcaat agctggaagc tgtgcagctg ctttggctcg
    33721 gtggtttaac tgcaataact gataaacgcc tttttgagct cgtgctcttg attctaaaga
    33781 gggcgtcgcg cttcctagaa ctattgggca atgatgatat ttactgcgcc aaattgctag
    33841 atctctcgca tgataacgcg gtgtctcatc ttgtttgtaa cttgattcat gttcttcatc
    33901 gataatgatc agcccgatat ttttcagcgg ggcaaaaata gcagatcgtg cgcctactac
    33961 tacttgcgct tcttcccgct caatcttgcg ccattcatca tatttttcac cttgggaaag
    34021 tccgctatgt aagaccgcaa ctgattctcc gaagcgtcct ttgaatcgct cgaccatttg
    34081 cggagtcaat gcgatttcag gcactaacat aatagccgtt ttattttctg acaaaacatc
    34141 agcgattgcc tgaagatata cttctgtttt cccgctgcca gtgattccct ccagcaaaaa
    34201 gacctgatct ttttgctgct gacctgctgt gatgatttgt tcgacagcat ttttttgttc
    34261 tgcattcagt tccaatgccg tcgtttggtc aaatactcgg tctttataag ggtcgcgata
    34321 acgttctgat tctataaact ccagccagcc cttttctttt ccttgattca agacagcagt
    34381 actgattcct ttatttttga aataagcaat tggttcttct tggctcagtt cagataaaca
    34441 caaaagaagt tctttttgtt tcttggcatt ttgccgtaat tttgcccatt cttcttcgat
    34501 ttgcttttca gttaacaaag aacggaccaa acgaatcgtt tttactttat ttcgtgtatg
    34561 gacttcatat ttgatatcta ctaattgctc ttggcgccag cgaatcaatt gagaaaggct
    34621 gccttcttct tctgcttcct tccaggaaat cacttctcgc tgatcaaaca atacctgaac
    34681 ttctggtgtg tctgccaaag gatagatcaa tttatcatat tctgctttca tcacactcgg
    34741 aagcatcgtt tgtagacaag tgattttgaa tgcgtaagta atttttttca tataatcagc
    34801 taatgaaagt aattcttcat tgaccaccgg agcaaggtct agcaaacgaa taactgcctt
    34861 caaagatgga ttagctgatt cttctgattt ttggatagcc ataacaaatc cttggacatg
    34921 acgattgcca ttaccaaaag ggacttctac tcgcatcccg acttgcaaag ctgtttccat
    34981 ttcagaggga accaaataag tgaatggctg atcggtctgc atagtaggta cgtccacaat
    35041 gacttcagct gttaatgtca tggttcgttt cctccttttt ttgtcttttc ttatcaagcg
    35101 acaaaaagtt ccttgcctat tttactagag atcgatggat ttgtcttatc ttctgccaaa
    35161 tcctaacaaa aaattaacag cagataagaa aaaaaagaac tgtgtcaaaa agttcagaca
    35221 cagccttttc taccagacaa taagtggcga atgtttgaaa aggaaacagc tgggtttgct
    35281 tttcttttta gatgttcgct ctatttttct ttattacatt ttttgctctt cacggatacg
    35341 tgattccaat tcgtgttgtt cacgctcttt ttgtgcttga cgttcctctt cttccatctt
    35401 caaacgttca cgttttaatt caggatgtgg atcattgact acatttcctg catcgatttc
    35461 ttctaaagct tgaccgacgc tttttactga atcaaaagaa tctagagtag gctgtgcact
    35521 tgcatccaat tcatgcgcgc gtttacttgc taaaatcact agagaatatt ttgaatttac
    35581 acgatctaat agtgagtcaa ttgaaggttt taacatcata agactacatc tcctttaaca
    35641 ttttaatata tttaccgatt acgcgatcta ctcgaaaatg ttcactggca ataatgtttt
    35701 tgattcgttc aactgctagc ggcacttcat catttacaac agcatagtcg taaagagcca
    35761 tcatttcgat ttcttctttt gcaactttca ttcgttcttc gattacttca tctgcatctg
    35821 ttcctcggcc tacgatccgt gattttaatt ccgctaaatc tggcggagtc aaaaagatga
    35881 atacaccatc gggcactttt tctttgactt gttttgctcc ttggacttca atctccaaga
    35941 aaacatcttt tccttcatcc aatgtcttgt tgacatatga caaaggagtt ccataataat
    36001 tcccgacata ttctgcgtat tccagcatct ctccagcttc gatcattgat tcaaactctt
    36061 ctttgcttcg gaagaaataa tcaacgcctt cgacctcccc ttcacgcatt ttacgggtag
    36121 tcattgagat cgaatactga aaatcattat cctcactctc aaaaattgct tttcgtactg
    36181 tgccttttcc tacaccggat ggaccagaca acacgattaa taatccacgc tctgacatga
    36241 cgacgtcctt tcaactagtc tttttgtact tatcgtttat tttgcacttt ttttctgcaa
    36301 ctttcaaggt tatctttaat tatagctaga aattggtaaa aaagctaccc tgtaccaaga
    36361 ggtcgtaaaa aagctgtcct gcatcaagta ataagaacag ccaaacataa acagcttctc
    36421 atgtttttcg tttggttggg gttattatcg cagatgcaag cttttgaacg ccgtgtataa
    36481 gaggtcgtga agaagccgat tagtttcgag tatgaagaaa aattttgtga aaatagtttc
    36541 tcacattttt gcataaattt ggcttaatac cgagaaaatg gtttttgaac atcacttatt
    36601 caaaataaaa aaagattgtg acacgaattt tgtcacaacc tttaaatgta ttcctagagc
    36661 aaagaagcta agcgaagaaa tccaattctg attatttatt gtatttcttt gttcagcatc
    36721 cttgctaaaa gcccatattg gacaaagtat tcactacacc catgatctca tcttttcgag
    36781 aacgtgcttt ttctgtcgga tcttctacct cttctgtgtc aattcgaaca ataaatttcc
    36841 gattcatcgt cacgtaagca ccgtcgttga tcaaaataaa cagttcgttc tcattatagt
    36901 ctgcacaggc atctttccat acgtcaaagc cgtttttttc agaagggata tcccgtttaa
    36961 ccacagtttt tccacttaat tcgtggaacg ttacgtcata atatttcatt atctctacct
    37021 ctttcgtcat tcaatatttt gtacttgttc tctgattttt tctaagatcg tcttagactg
    37081 gacgacttga tttttaattt cgattgcatt tgatttggaa ccgatcgtgt tgatttcccg
    37141 attcatttct tggatcagga aatctaattc ccgacctaca ggacattgtg cttcgataag
    37201 ttcatctagt ttttgtacat ggatcgccag acgatccagt tcctcgtgaa tatctccgcg
    37261 ttccaacaag atggctagct ccgtcaacaa ccgttgctca tcgactttcg ctccaagaaa
    37321 ttcctccagc tttttttgat accttatttg aaattccttt tcatagattt ctacaaaact
    37381 atcgagtttt tttagtactt ctttgaattc ttcagtattc tttttcaaca ctaaagctaa
    37441 agcttgacct tctttttctc gactcgcctc aatagaatca agtgcgcttt tcaatgtatc
    37501 tagagctaac tgtgccaatt cttccatgct ctcttccggt ttttcttcta ccgttacgaa
    37561 gttttcgtcc agcgcgaagc gttccgccat ccgactaatt gacacttgat tttgactacc
    37621 gtatcgttgt tgaattccgt ctgtcaattg agtgataagt gaatcaatca attcccagtt
    37681 gatgacgatc tgtttttcgt ttttgccaat ataagctaga ttcacaaata cttcgattcg
    37741 accgcggctt aactgctgtt taataagctg acgaaaatcg ttttcaacaa agttcaggat
    37801 tttaggactt cgtatttgaa catctagaaa acgctggttg acacttttta tttcaatatc
    37861 taactgatac gcttttgttt cgcggatggc tttgccaaaa ccagtcatac ttttcattcg
    37921 acagggtcct tcctcattac atattttcta ttgctttttg cagttgttca aactcttcgt
    37981 tcgtcaatgt caggcctttc cccatttttt catggtctgg cgcccaatct cgaagatcga
    38041 attttggcgg gcgaccattc cagctgacta ggttcagttc tttacgccac cccttgttgt
    38101 tttctgacaa tatagcgatt tcttctacga tttcatatga aaattcttgt gccatggttg
    38161 ttcctccttg agataatata aatataggat cagtaatgtg tcaactggtt gcttaagcaa
    38221 ttcatgctgc aaacgatcct tgcgttttcc ttgtagcaga taatcaaaat aaacagtttt
    38281 cgtgcgctca ttttgtattt tttgctggta ttttcgatac cttcgccatt cttcagtact
    38341 taattgacca atttgtgcca ataaatcatc cggcatactt tttatatctt gttggcagat
    38401 catttgaatc atcatgtcta cttgatagat ttcattgggc agtaaaaaat gtttttttgc
    38461 taaaagttct agcatcgcca atccgcatgt ctctggtacg accctgttct tatataaatt
    38521 acgcagtttt tgccaaaatt ttatgctttt gaaaaaagat aattctaact gaatttcttt
    38581 tgttaagtaa gacaaaaatc ctcgccagta tgttcttgca ggtttaacag aatgaaatgt
    38641 ataccagttc ccatagtaat agcctaatcg ttttttcatt tccagatagc ctaattgtaa
    38701 atttaattgg ttcctttggc tatcaaaaag cagaaagctg cctagtgtcc acaatgtttg
    38761 acatttccaa tggataaaaa tgtctggcat acgaattttt ttcgctgggc ctggcccttg
    38821 cacatctacg acaaatgcct ctgttgctcc tttgttaatc gcaatatcaa taggtatttt
    38881 gttgcgatac ccaccgtctg catatttatt ttttccgatc tttcgataag ccatagcggg
    38941 ataaaatgaa gcagaggcga gtatccaatc tggtatttct tctgtttttg tctgttggat
    39001 atcagtcact acttccgcta gttctggcag tttagtagac accgtatata aacgtatttt
    39061 gggattatgt ttgagtaagg acaaatccaa attgtctttg accatctttt ccaatggagc
    39121 agaagaaacc ccgccttcaa ttagtgctgt tttcgtcatt tggcgtcttt ctcgatagaa
    39181 gcgttcacga aggtcttcaa ctgcagccat ttctggtaac gcaagcactt gtcttgtcga
    39241 cagctttttc cataaattca cagctttttt catatctcct tgtaaaatca atgcgccgtt
    39301 caacgcacct accgaggtac ctgtgataat ctcaaagttg atatcatgtt ccttcagcgc
    39361 ctgccacaca ccaatctgat aagcaccgtg tgctcctcct ccacctaaaa caagtgctgt
    39421 atgatagacc agctttttat ttaattgtgt ggttccttga tagccgtttt tatgaaaaat
    39481 acgcaaccat tcttctgata gaggagattg aaataaaaaa caacagtttt ctttaataaa
    39541 aaaacgagcg caagcttcca tataccgaaa aaattttttc cattcaaaag tttcaccttc
    39601 aatcaacaga ttattaaaaa taacatctcc tctactttta gcaatagtaa aatagacggt
    39661 atattttttc gatactgctt taaaagcaca ttgctttttt gcagaacgcc aaaaagcgtc
    39721 tctactttct tgatgacttt gaaaaaatgg aaggggttgt tttgcttgct ggataggcga
    39781 atacagcgac attttctgtg aaaagtaaac tttttttatc tccatcgcgc tctccttttt
    39841 cttcaagtat accagtacgt cataaaaagg tcatgagtct cgtattcgac tttgtagatt
    39901 ccataagaaa acaattcata aaacatcctt taaataaaaa atacaaaaat cacaaaaatt
    39961 aattgaaaat gagagtgttt tcaatgaaaa cgtgattttg taatggaatt ttaaaataaa
    40021 agaaacaagc ttttttaatg caaatgttgt aaaataaaag agattaagag gaaataagag
    40081 gaggaacatg atgactgagt cagtttatgt acatattgat accacaagta atgctgtttt
    40141 aacaaaaggg ctgactgcta ctgactttgc aaacagtatc gtgcattttc ctcaaaactt
    40201 gctcttgctt gatccatctg cttctgctgg agaatatgaa tcccatacag ggctgaaagt
    40261 aatccgtggt acagaaaata ttcagcgttt cttttcctct tctcgtaaca ggcgtggatt
    40321 cgatgaattg aagtggatcg actttactga tttaacgatg ctgaaagaat tgacaccttt
    40381 agaaatttca gaattattat attttggtca tatgaaaaca catcttcatt cgccgttttt
    40441 ttataaacta cagaataatt ttgtatactt cgatcttaat gatcagctga atcggattta
    40501 ttatcgctat ttagatgaat tttataggat ttttgctagt aaattaagtt cgatcttgtc
    40561 agataagatc aatgtaaaaa aatctttttt caaaaaagaa acacctgttg aaaaaatcag
    40621 tgtggaatta ttgaaagaaa tgcgtaattt gatgcaggaa gggatcattt tttcttttga
    40681 tcaagttggt ttgatcgatg gtaaatatcg tatcccaatc tatgtgattg aagattatat
    40741 ttggaaatta aaaggcaccc ggtatcgaga agaggaaatc atcgctactt taatctactc
    40801 tgtcaccgat cgacactggc aattggtaga agatgccgat ctttcttatc tgccattgaa
    40861 gtagatctat ttcttatcaa aaaataaaaa ggaaacaaga aactaggaca tgattctgtc
    40921 tcaagtttct tgcttctttt ttatttacag ctcctgtcgt ttatctggca caaaaatcga
    40981 aggagtcgtt tgttggatcc ctaataaaac gatgatcgtt acaatagctg tactcaaccc
    41041 gctggcacca ttcattacga aagaaaagag ccaagaattc atcccccata gtgcatacgc
    41101 tccccagaaa accactcctg caataaaatg ccaaaaatat ctggaaagtg cgccaatcaa
    41161 tgcaccaaac acgatgcttt ttctaatttg tttcgtatta tcgtctacat agcctttttg
    41221 gacatttgaa gccatgagcc ctgctgcgcc tgcaaaagta taggcaatcg ggtactcaat
    41281 caacacttga acgacactta aataataaac ttgtcccaac ggaaaatgta ggatgcccca
    41341 aatgaaacct gccagcatcc ctgcctttgt ccctcttctt aacgcaaaaa gagtcaatgg
    41401 aatctgccct aatgaaatag aaaaactgct gccgatctgg atcggaatca acgacaacac
    41461 catcgctaac gctgcaacaa tcgttccttc tacccaaact ctattttttc ccataaaaaa
    41521 acttcctcct ttgaataaaa aagtcacaaa ggaggaagca gctttcgaaa atagtcaaac
    41581 agctgtttat tcacaattcc tacgctcgca taaccgatac aggtacgaag ggtttagaaa
    41641 tagattcaat ctcagcccaa ttggactccc ctttgtgagt ttatttatta tttatttttt
    41701 gtttcaaaca atggactaac tggtttgttt tcatggatac gtttgattgc atctcccatt
    41761 agtccgccaa cacttacttc atcgattttg tcgattttac gatcttctgg aagatagatc
    41821 gagtctgtaa caactagacg ctcgatagct gaatcttcta ttctttgtaa agcagggccg
    41881 gataatactg gatgtgtaca tgaagcatac acactagttg caccagcttc tttcaaagca
    41941 ttggcagcca atgtgatcgt acctgctgta tcgatcatat catcgatcag tacacaagtt
    42001 ttgcctttga cttgtccaat gatattcatt acttctgcaa catttgcttt tggacggcgt
    42061 ttgtctatga tcgcaatcgg agatttcaaa aattccgcta atttacgtgc ccgtgtgaca
    42121 ccaccatggt ctggtgaaac aacgacaaca tcatcgcctt tgattccttt ttctaagaaa
    42181 taattggcaa taagtggcgc acccatcaaa tgatcgactg gaatatcaaa gaaaccttgg
    42241 atttggactg catgaaggtc caaagtcagc attcttgttg caccagcttt ttgaatcatg
    42301 tttgctacta atttcgctgt gattggttcc cgtgcacgtg ctttacggtc ttgacgcgca
    42361 tagccataat aaggcatgac tacgttgatt gtttttgcac ttgctcgttt cagtgcgtca
    42421 atcatgatca gcaactccat caagttatca ttgactgggc tgctcgttga ttgaataaca
    42481 tagacatgcg atccacgaat actttcttca atgtttactt gaatttctcc atcactaaat
    42541 tgagtaacag atgattttcc taactcgacg ccgactgctg ctgcaatttt ctctgctaat
    42601 gggcgattgg aatttaacgc aaaaattttt aatcttgggt caaagtaatg ttttgacatg
    42661 gggacctcca ctttcttttt cacaactcta gaattctacc taataaatag tagtcatatt
    42721 ttttaaataa atcaagtgat tttgcaaaag tcttagttta gataagggag tttttttgca
    42781 tatccttctt tattgacttg tcttgctctc gcaattgcca gcgcatattc tggaatattg
    42841 tctgtaattg ttgacccggc agcaatcgaa ctgtttttag ctacctcgac aggtccgatg
    42901 atattcgtgc tagaaccaat aaagctgtgg tcgccgacag tcgtatgatg tttgttcttt
    42961 ccatcatagt tgacgaagac aacaccacat ccgacattga tttcttctcc cagcgtagca
    43021 tcaccaacat aagtcaagtg accgacttta gttcctttgc cgatttgagc atttttcact
    43081 tccacaaagt ttccgatatg gacgttttcg ccaatttcag cttttggtcg taaatgtgca
    43141 taaggtccca catctgcatg tttcttgaca tggctgcttt caatgactga gttttcaatg
    43201 acgacgtgat cctcaatcct actgtctacg atttttgaat gtgacccgat gacacaatca
    43261 gaaccaatga ctgtatttcc ttggatttgt acacctgctt cgatcaatgt gtctggaccg
    43321 atttctacgc ctgcatcgat gtatgtcgtt gctgaatcga caaagcttac gccattgacc
    43381 atatgcatct ggttaattcg ctttctcatg atctcatttg ctttagctaa cgcgatccga
    43441 tcattgactc ccattgattc ttcaaaatca tccgtttgat aagcagcaac tgtatggcct
    43501 tcttctttta agatttcgat gatatctgtt agatagtatt caccttgagc attgtttgtt
    43561 ccaactttgc ttaacgcatc gaacaaagct tcgttgtcaa aacagtaagt tcctgtattg
    43621 atttcttgta caagagcttc ttctggtgtt gcatcttttt gttccacgat tttttctacg
    43681 atcccaatat ggtcacggat gattcgtcca tatcctgttg ggttttctgc ttgtgccgtc
    43741 aagatcgttg cacttgcatt ttttccttgg tgatattcaa aaagattgtt cagcgtttca
    43801 gtagtcaata atggtgtatc gccactgata actaacgttg ttcctttttt tccttccaag
    43861 aaagaagcag cttgcaaaac agcatgtcct gttcccaatt gttctgcttg taacgcatat
    43921 ttactgcgtt ctcctagttg tgctttaact tgttctgctc cgtgtccaac gatcgtaatc
    43981 acttcatctg gtttcgtttc actgacccga ttaatgatat gttctaccat aggtttacca
    44041 gaaacgggat gtaaaacttt atataatttt gacttcatgc gggtaccttt ccctgccgcc
    44101 aaaataattg cataacgtgc gtccaactgc gtcactccta aatatagttt gctgatttca
    44161 gtttatccca gtatacccat tttttcaaca aaacaagtga ttttctttta tcccagcgtt
    44221 ttctggataa aaaaatagat agaccataac gggcctatct atttggtaac cggtgctctc
    44281 ctgccaactc ctcggtcaca agtcacacta ctcataaaac atgaaaagct gttttttgaa
    44341 atcgtttctt gtgacttaga gttaaacggt aggcttacaa cctcttcatt aaacgttttt
    44401 caatcagctg accttcggta ttagctcaat aaacggcaaa atatgaggag caatttttcc
    44461 gtttattgag ctaatactca ggacaggacg ctgatttcac aacctctaga taaacggtgt
    44521 tcaaaagcca gtttctcggt attaagccaa atttatgaaa aaatgtggaa agccattttc
    44581 acaaaatttt tcttactact cgaaactaag cggctttttc acaacctctt attctgaaaa
    44641 ataatttcct ggcacaaccg taatattttt cgtttgagta tctacatctt tgacataaag
    44701 cagtgacgta taatcatcga tggcgcgatg accgttgaat tttgattcgg caaatacagt
    44761 gatgccaaca agttctgatt cgaattcttc aatcaagctc ttcattccgt tgactgttcc
    44821 gccgcctttc atgaaatcat ccactacaag gacacgtgaa ccacgtttca aacttctctt
    44881 agacagttcc atcttttcaa ttctttcaga agatccagac acatagttga cacttactgt
    44941 tgatccttct gttatttttg agtcccggcg tacaataacg aatggcgcat tcagataata
    45001 ggacacagct tgggcaatag gtacgccttt tgtggctaca gtcattacag catcgatttt
    45061 ttcaccaagg tatttagaag caatgattct tccaacttga cgcagcagtt caggttctcc
    45121 taacaaatca gagagataaa catatcctcc tggaagcaga cggtcttgtt ctgatagacg
    45181 ttctgctaga gattcaatat attttttcgc ctcttcatat gaaatttcag ggatgaacaa
    45241 tacaccacct gcagcacccg ggattgtttc caaggttcca taccctcgtt ctttaaatgt
    45301 tttcttgatg attcccaaat cttcactgat agaagattta gcagattcat aacgttcggc
    45361 aaagcttgtt aaggaaatca attcatgcgg gtgattcaat aaatactgtg tcatgtcaac
    45421 tagacgttca ctgcgacgta ctttcaccta ttccaactcc ttttaagaaa ttttatcgtt
    45481 ttacattata taagtttttc ttcaaaaatt cgagcatttt atgaaaaaat aagaaaatag
    45541 ttcggtattt attaaaataa aaagaagcct ataaaaaatt tttcgttttt ataaggcttc
    45601 ttttctttat aatttagtta ttttttcttc tgaaggcaac caatattcgt ttgatgatat
    45661 ttacaatcag gaacaaagca ataaagacta atgtgattgt tgctcctgga ggtgtatcta
    45721 gataaaacga gctagttagt cctgacagca tccctagaaa tcctacacaa acactgatag
    45781 caatcacgac attgaagctt ttgcctaatc gcataccgat tgctgcggga agaaccatga
    45841 ttgcggatat caaaagtgca ccggcaatcg ggatcatcac agcaatagcc acaccagtaa
    45901 tcacattgaa catcatcgac atcaaatgaa cgggtagccc atccacgtgg gcggtatctt
    45961 catcaaaagt cagaacatac atcggccgtt tgaataaaaa gaacaatata ccgatggcaa
    46021 cgaataagat tcctaaaaag atgacttgct cccatgtaat ggtcacgatc gaaccaaaca
    46081 gataggattg gatacttgtt gctgaattcc ctccgctcaa attcatcaat actaacgcta
    46141 gcgccaaacc accagccatt aaaatggcaa tggagatttc tgaataggtt cggtagatcg
    46201 ttcgcagata ctccaatatc acagctgcta atacaacgac tagcatggtc gtaagtgtag
    46261 gattaaggtt caaaaagaat cctaacgcta cccctgctaa agagacatga gaaagcgtat
    46321 cagccatcag cgattgtctt ctgatcacta aaaacacacc tagcatcggg gcgattccgg
    46381 caatgaagat agcggcaaga aacgctcttc tcatgaattc atatgaaagc aacgccattg
    46441 agaatcctcc tttctcacca gtcggatctg tctgtccgca tactgtttga tatcttcatg
    46501 atcatgggtg atcattagaa ctgatttccc atggacatgc gcactatgtc ggagaagacg
    46561 ataaaattca tttctggatt cttcatccat acctgttgtt ggttcatcaa gaacaaataa
    46621 gtcagggtca gtagcaaaaa tacgcgctaa actgattcgc tgcttttgtc cgccagacaa
    46681 ttcacctatt cgccgatttc gcatatccca catccctaca gcttctaaag cacgctgtac
    46741 atgttgatga tctttttcag agagacgctt gaaccagcgg cctcttggat atcttcctga
    46801 acggactaat tcgatcaccg tactaggaaa acctgcattg aaagatgcaa cttgttgagg
    46861 aatataaccg atacttaatt tttttccttc ttgatttttc tcagcaatct ttatcattcc
    46921 ttttgaaggt ttcagtaatc ctaacgatgc tttcaccaag gttgacttgg ctgctccatt
    46981 ttctccagtt aaaatgacaa attcacccgc atctacatga taagagacat cttctagtac
    47041 aggttcatca tcatagtaaa aggtcaaatc agatatttcg atataacgca ccttacaacc
    47101 tccattctaa aacttaattg atactttctt ttaaagcttt cagatttttt tccatgacag
    47161 aaatataatt ttcgcctgct ttgatttgtt cattagtcaa gctttctaaa ggattcaaca
    47221 cttctagctt cactcctgtt tcattcgaca atgtctcagc taccttcgaa gaggcatttt
    47281 cttcaaaata aatcactttg atgttgtttt tcttcacaaa ttcttttagc tcagctaagc
    47341 gacttggtgt gggttcctga tctggtgaaa cgcccgaaat cgctacttgt tttaagtcgt
    47401 attgattcgc taaataagca aaagcagcat gctgagtgac aaatgtgcgg tttgttgcat
    47461 cttttagctc atcggagtat ttttgatcca gtgcttcaag ctttttgata taagcatcgg
    47521 cattcttcgt aaaagtttcc tttttatctg gatatttttc gcctaattct tcagcaatcg
    47581 ttctgacttc ttttatagct aataccggat cgagccaaac atgtggatcg tattcatgtg
    47641 aatgaccatg cccttcttct gattcttcat gttcgtctgc ttcttgcgat tctaaacggt
    47701 cgattccttt agctgcttcg atcatcaagg tctgtttgct atccactgaa tcctttaacg
    47761 aatccacaaa aaattccatg tctgaactat tatacacgaa aacatctgca tcggaaattt
    47821 ctgctctttc ttttgcactt ggttcgaaat catgcggctc cgttccagca gggatcaaaa
    47881 gtttcacgtt tccttcatcc cctacgatct cttttgtaaa atcatacatc ggataaaaag
    47941 tcgtaacgat cgtcaaatcc tcttttttgt ctgcttcctt gttcgtattt ccacatgctg
    48001 caaacagtaa agcacctatc attaccgtag ctgtcaaaag atattttttc atttatatgc
    48061 tcctttcaat gaacaaatcg gaattttacg atttattaac gtgaactaat gtatcaaaat
    48121 aaaaaaacac agtcaataga aatccatcaa tttctgattt aagcatgtat attcttgatt
    48181 aaatcgtaaa atggatgagt tgctgctcat a
//
LOCUS       DAFEOM010000013        47996 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.013, whole genome shotgun sequence.
ACCESSION   DAFEOM010000013
VERSION     DAFEOM010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.013"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(22..1806)
                     /gene="pyk"
                     /locus_tag="LP524_000895"
     CDS             complement(22..1806)
                     /gene="pyk"
                     /locus_tag="LP524_000895"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBL2233966.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(1880..2842)
                     /gene="pfkA"
                     /locus_tag="LP524_000896"
     CDS             complement(1880..2842)
                     /gene="pfkA"
                     /locus_tag="LP524_000896"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBL2233967.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(3010..6324)
                     /gene="dnaE"
                     /locus_tag="LP524_000897"
     CDS             complement(3010..6324)
                     /gene="dnaE"
                     /locus_tag="LP524_000897"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBL2233968.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            6555..6740
                     /locus_tag="LP524_000898"
     CDS             6555..6740
                     /locus_tag="LP524_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HBL2233969.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(6802..7998)
                     /locus_tag="LP524_000899"
     CDS             complement(6802..7998)
                     /locus_tag="LP524_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBL2233970.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(8168..9604)
                     /locus_tag="LP524_000900"
     CDS             complement(8168..9604)
                     /locus_tag="LP524_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBL2233971.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            9897..10706
                     /locus_tag="LP524_000901"
     CDS             9897..10706
                     /locus_tag="LP524_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL2233972.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(10892..13045)
                     /locus_tag="LP524_000902"
     CDS             complement(10892..13045)
                     /locus_tag="LP524_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBL2233973.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(13440..14288)
                     /locus_tag="LP524_000903"
     CDS             complement(13440..14288)
                     /locus_tag="LP524_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL2233974.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(14512..16452)
                     /gene="thrS"
                     /locus_tag="LP524_000904"
     CDS             complement(14512..16452)
                     /gene="thrS"
                     /locus_tag="LP524_000904"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBL2233975.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(16789..16968)
                     /locus_tag="LP524_000905"
     CDS             complement(16789..16968)
                     /locus_tag="LP524_000905"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HBL2233976.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(17015..18838)
                     /gene="cadA"
                     /locus_tag="LP524_000906"
     CDS             complement(17015..18838)
                     /gene="cadA"
                     /locus_tag="LP524_000906"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBL2233977.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            19116..21395
                     /locus_tag="LP524_000907"
     CDS             19116..21395
                     /locus_tag="LP524_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL2233978.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(21488..22135)
                     /locus_tag="LP524_000908"
     CDS             complement(21488..22135)
                     /locus_tag="LP524_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2233979.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(22314..23033)
                     /locus_tag="LP524_000909"
     CDS             complement(22314..23033)
                     /locus_tag="LP524_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL2233980.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(23164..24315)
                     /locus_tag="LP524_000910"
     CDS             complement(23164..24315)
                     /locus_tag="LP524_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBL2233981.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(24532..25272)
                     /locus_tag="LP524_000911"
     CDS             complement(24532..25272)
                     /locus_tag="LP524_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL2233982.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(25276..25599)
                     /gene="rsfS"
                     /locus_tag="LP524_000912"
     CDS             complement(25276..25599)
                     /gene="rsfS"
                     /locus_tag="LP524_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBL2233983.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(25656..26249)
                     /gene="yqeK"
                     /locus_tag="LP524_000913"
     CDS             complement(25656..26249)
                     /gene="yqeK"
                     /locus_tag="LP524_000913"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBL2233984.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(26233..26883)
                     /locus_tag="LP524_000914"
     CDS             complement(26233..26883)
                     /locus_tag="LP524_000914"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBL2233985.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(26893..27204)
                     /gene="yhbY"
                     /locus_tag="LP524_000915"
     CDS             complement(26893..27204)
                     /gene="yhbY"
                     /locus_tag="LP524_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBL2233986.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(27220..28329)
                     /gene="yqeH"
                     /locus_tag="LP524_000916"
     CDS             complement(27220..28329)
                     /gene="yqeH"
                     /locus_tag="LP524_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HBL2233987.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(28326..28853)
                     /locus_tag="LP524_000917"
     CDS             complement(28326..28853)
                     /locus_tag="LP524_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HBL2233988.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(29033..29818)
                     /locus_tag="LP524_000918"
     CDS             complement(29033..29818)
                     /locus_tag="LP524_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBL2233989.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(29811..30680)
                     /locus_tag="LP524_000919"
     CDS             complement(29811..30680)
                     /locus_tag="LP524_000919"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBL2233990.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(30681..32060)
                     /locus_tag="LP524_000920"
     CDS             complement(30681..32060)
                     /locus_tag="LP524_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBL2233991.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(32068..32487)
                     /gene="fabZ"
                     /locus_tag="LP524_000921"
     CDS             complement(32068..32487)
                     /gene="fabZ"
                     /locus_tag="LP524_000921"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBL2233992.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(32512..32988)
                     /gene="accB"
                     /locus_tag="LP524_000922"
     CDS             complement(32512..32988)
                     /gene="accB"
                     /locus_tag="LP524_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBL2233993.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(32991..34226)
                     /gene="fabF"
                     /locus_tag="LP524_000923"
     CDS             complement(32991..34226)
                     /gene="fabF"
                     /locus_tag="LP524_000923"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBL2233994.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(34245..34982)
                     /gene="fabG"
                     /locus_tag="LP524_000924"
     CDS             complement(34245..34982)
                     /gene="fabG"
                     /locus_tag="LP524_000924"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBL2233995.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(34994..35911)
                     /gene="fabD"
                     /locus_tag="LP524_000925"
     CDS             complement(34994..35911)
                     /gene="fabD"
                     /locus_tag="LP524_000925"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBL2233996.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(36018..36242)
                     /locus_tag="LP524_000926"
     CDS             complement(36018..36242)
                     /locus_tag="LP524_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBL2233997.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(36295..37257)
                     /locus_tag="LP524_000927"
     CDS             complement(36295..37257)
                     /locus_tag="LP524_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBL2233998.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(37267..37707)
                     /locus_tag="LP524_000928"
     CDS             complement(37267..37707)
                     /locus_tag="LP524_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL2233999.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            38013..38978
                     /locus_tag="LP524_000929"
     CDS             38013..38978
                     /locus_tag="LP524_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBL2234000.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(39035..39265)
                     /locus_tag="LP524_000930"
     CDS             complement(39035..39265)
                     /locus_tag="LP524_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HBL2234001.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(39351..40238)
                     /locus_tag="LP524_000931"
     CDS             complement(39351..40238)
                     /locus_tag="LP524_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBL2234002.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(40327..41550)
                     /locus_tag="LP524_000932"
     CDS             complement(40327..41550)
                     /locus_tag="LP524_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBL2234003.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(41567..42613)
                     /locus_tag="LP524_000933"
     CDS             complement(41567..42613)
                     /locus_tag="LP524_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBL2234004.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(42777..44504)
                     /gene="ptsP"
                     /locus_tag="LP524_000934"
     CDS             complement(42777..44504)
                     /gene="ptsP"
                     /locus_tag="LP524_000934"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBL2234005.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(44504..44770)
                     /locus_tag="LP524_000935"
     CDS             complement(44504..44770)
                     /locus_tag="LP524_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HBL2234006.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            45249..47483
                     /locus_tag="LP524_000936"
     CDS             45249..47483
                     /locus_tag="LP524_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL2234007.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQKAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            47631..47951
                     /locus_tag="LP524_000937"
     CDS             47631..47951
                     /locus_tag="LP524_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBL2234008.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT        13691 a        10309 c         8401 g        15595 t
ORIGIN      
        1 tttatttttt attaagctaa attagatagc tgttgtttcg ccacggtaaa cgatgccgcg
       61 acgtgaatca acggtaatca attcaccttc tgtaattgtt gttgttgcgc ctttagcacc
      121 aacgattaca ggaatatctt gtgcaattcc tacaactgca gcgtgagaag tcaatccgcc
      181 ttcttcaacg ataacagcag ctgctttttc gattgcaggc atatattctt tgtcagttga
      241 tggtacaact agaaccattc cgtcttttgc tttttttaca gcttcttcag cagatgaagc
      301 aataactgca tttgcaacga cagatttttg tccaacgcct tgagcttcta ataatttaga
      361 accaatcatt tgaattttca tcacgttagt tgttccgcgt tcaccaactg gtacaccagc
      421 agtgatcaag attaaatcgc cttctgatgc gaaacctaat tcaacagctt ttttagctgc
      481 tagttcaaac atttcatcag ttgttgatgg tttttcagca actgttgggt aaacgcccca
      541 gtttaacatc aaaccacgtt ttgtgcgttc gtcaaaagta acagccaaga tatctgcatc
      601 tggacgatat tttgaaatca tttttgcagt gtatcctgat tcagttgcag caacgattgt
      661 tttcacgcct agattttttg cagcacgagc tacagataaa ccgattgttt cagttacatc
      721 tgttttatcg aattcgttca attggaatgt acctaattct gataatgcat tttcagcttc
      781 ttggtcgata cgtgccattg ttgaaacagc ttcaactggg tagtccccgt ttgcagattc
      841 accagatagc attgttgcat ctgtgccatc aaatacagcg ttagcaacgt cagaagcttc
      901 cgcacgtgtt ggacgtgggt tttcttgcat tgattctaac atttgtgtag cagtgataac
      961 tgggatacca gcagcattac attttttgat aatgcgtttt tgaaccattg gtactagttc
     1021 agctgggatt tcaacaccca tatctccgcg ggcaatcata acaccgtcag aaactttgat
     1081 gatttcgtca atgttgtcga taccttcttg agattcgatt ttagggaaga tttgaacatg
     1141 agtcatgtct ttttcttcta agatttcgcg gatatctaga acgtcttgtg ctttacgtac
     1201 aaaacttgca gcgataaagt cgatatcgtt gtctaaacca aaacgaatat cgtcagcatc
     1261 ttttggagta atacctggca aactaataga aacgcctggt gcatttaccc ctttacgtga
     1321 tcctagcata cctgcatttt taacttctac gactaattca cggtttgctt cgtctttttc
     1381 aatgatttgc atatcgatca aaccatcatc aaataaaatg tgtccgccaa cgtgaacatc
     1441 gtcaaataat cctgggtaag taaccgcgat tttttctttt gaacctacat gttcaggatc
     1501 catagagatg cgtgtttgat ctcctacttc aaatttgatg tatcctgcac gaccaaaatc
     1561 agcttctgtt gtgccttgaa cagttgtacg gatttcagct ccttttgtat ctaaaagaat
     1621 accgacatct tttcctgtct ttttaactgc ttcacggacc atagtcatac gtgataattg
     1681 ttcttcatgg tcaccatgag aaaaattaaa gcggaagaca tttgccccag attcaatcaa
     1741 ttgagaaata atttctactg agttacttgc tggtcctaat gtactaacga ttttggtttt
     1801 tttcatttaa aaaactctcc cattcaattt tgtttattta aagctgtaca cagcttaaat
     1861 agcttggttt ctttcactct taaaatgaaa gtgaattatt tagatcatat agtgataagt
     1921 ctggtttatg tttgttgttt tcaagtgtat caatgatgtc agacgcaacg atttcgttgt
     1981 catggatacc gacacataat ccaccttttc cttcttgaag caattcgaca gcgtaagcac
     2041 cgaatttact tgccaacacg cggtcgcgtg cgcttggtgc tccaccacgg acaacgtgtc
     2101 ccaagataga tacacgtgta tgatagtcac caaattctga aagtttctca gcgaattcat
     2161 ttccgcccat tacaccttca gccaagatga tcaagcaatg tttcttcccg cgatcgcggc
     2221 cttcttggat ttttttagca actgaagcca tatcaaaatc atgttctggg atgatgattt
     2281 catccgctcc gccagccaca cctgaccata aagcgatgtc tcctgcattg cgtcccatta
     2341 cttcgataac gaatgtacga acatgagaag tcgctgtgtc acgaatacgg tcaattgatt
     2401 ctagtactgt attgattgct gtatcaaaac cgattgtgaa atcagtccca ggaatatcat
     2461 tatcgattgt tccaggaata cctactgctg ggaaaccgcg ttttgtcaaa gccattgcgc
     2521 catgataaga gccgtctcct ccgataacca ctagaccttc gattccgaat tttttcaatt
     2581 gttcgattcc ttttaattga ccttcttcag ttgcaaattc tggataacgt gcagaataca
     2641 agaatgtacc accacgttgg atcttgtcac ctacatctgc cacatcaaga cgacgaatat
     2701 ctccagcaac taaaccggca aaaccataat tgatcccgta tacttccagt ccttcgtaaa
     2761 tacctttacg aacaacggca cgaacagctg cattcattcc aggtgcgtct ccgccactgg
     2821 tcaaaattcc gatgcgtttc ataataaaca ttcacctcat taaaatactt tatcctaagt
     2881 atgacttagg tttacaacca tttttgcgtt aaccacgact aacattctaa cattattttt
     2941 ctcacttgtc ttgatttacg gataaaaaaa tgaaaaaaaa tgaaagcaag tagtattttt
     3001 taccgtaagt tatttgaaaa ccacattatc ttcgccaagc agataaataa gttgcccctt
     3061 tatactcttg ctgtcatcca cccaataagc atctgacaga actacttttc gttgcgtttt
     3121 ttcgtgatac ataactactg gtatatatcc tgggtgtttc tgaaaaatca cttgtaattg
     3181 tttcattatc tcgggatctt cccgttcttc tgtaatacgc aaatagcaag tttgtgaagg
     3241 atactgggct tctaactggt taggatcagc aaagctttct ccaattagtt gcagttcctg
     3301 attatatttg ctgatttcgc tccgcccttg aacaaagatc actcgatcaa cggcaaacaa
     3361 attacgatat ttacgataaa gagttggaaa aactgtgaca gaaatttctc cactactgtc
     3421 ggttccttca acaaaggcca tcagttctcc tttttttgtc cgaatttctc taacttcctt
     3481 gatcatcact aataaattga ctggttgatt agggacgata tctgtaatca gctgaacttg
     3541 tttagctaaa cgaagacgat caaacccttc tgcaggatga cctgacaaat aaatacctaa
     3601 gtactcctct tccagcttca agcgttcttc tagtgtatag tcagcgactt cttcttcttt
     3661 taatgccatg attcccagca agtcaagact tccaccactg taaacgatat tttggatttt
     3721 tccttcgaga tcaagcatca attgtttcct gttaggagat aattcatcaa aaactcctac
     3781 tgctgtcagt gattgcagta actctaattt cagccaacgc ttatcgatcc gtatgaggaa
     3841 ttgatccact gactggaacg ggccattttg ttttctttcg tttaatatat cttctacaaa
     3901 atcacgccga atacctttta tcgctgtcag tccgaatctg atttcttcct tattgaccaa
     3961 ctcaaaactg gaattacttt gattaatgga cggcgaggaa agtttgagtt tatttttacg
     4021 tgcttctgca atatattctt tcaattttga tgtgttatgc cgaacagaat tcatcaggct
     4081 ggtaaagaaa gcacctggat gatgggcttt tagatacgcc atttggaatc ccacgaatga
     4141 ataagcaaat gcatgtgacc gattgaatcc atagtttgca aatttttcga tatattcata
     4201 tacggtcgct gctgtttttt ccgaatatcc ctgttgtaat gctcctgata caaaatgcga
     4261 cttttcttca tctaatacgt cttttttctt tttactcact gcccgtcgca gaatatctga
     4321 ctgccctaat gtaaatccag ccatagtcga ggcgatctgc atgatttgtt cctgatagac
     4381 gatcactcca tatgtgtttt tcaaaatcgg gatcagtgaa tcatctgggt agcgaatggc
     4441 ttcttttcct ttcttcctgg caatgaaggt atctatattt tgcatagggc ctggtcgata
     4501 caatgcattg actgcggcaa tatcttcgat gttttctgga tctaatcttc gtaaaacgtt
     4561 ccggatacca gcagattcaa attgaaatac tcctgaggtt tcgcctcgtt gaaacaagag
     4621 cagcgttttt gcatcattta gcgggatctc ttttaatcgg atctcatacc cttctactcg
     4681 cttaacagct ttcaaagtaa aatcaataat cgataagttg cgtagtccta aaaagtccat
     4741 tttcaaaaga ccgattgctt ctacctcatt catcgtatac tgggtcagaa aagcttcatc
     4801 agaaccgcgt tggagcggaa ctagttcaag tagattctta tcgctgataa ctacacctgc
     4861 tgcgtgagtc gaaacatgtc ttggcaatcc ttctaaaatc actgcagttt tgaataaaag
     4921 ttggtttttt tcgcttgcct gaactaattc tactaattga tgcgaagttt tataagcttg
     4981 ggtcaatgtg attttcaatt gattgggtat tgcttttgac cagcggtttg cttcactttg
     5041 cgataaacca aacactctag ctgcatctct aagaaccatt tttgctgcca ttgtgccaaa
     5101 tgttgcgatt tgtgccatat gatattgtcc atacttttct cgaacataat tcaatacttc
     5161 ttcacgtcga ttgtcaggaa tatccaaatc gatatctggc atcgtaaagc gttcggggtt
     5221 taaaaagcgt tcgaacagca aatcatattc taatggatcc acatctgtga tttctaacac
     5281 ataggctact aatgaacccg cagcagaacc acgacctgca ccggtaacga ttttgtttct
     5341 atgagcaaaa gccattacat cccatacgat caagaaataa tcatcgaatc ccatttgatg
     5401 gatgacagat aactcgtagt ccaaacgttc ttggtattcc tttgtatagt cagtgattcg
     5461 gcgttccact tgcgaatagc ataactctct taaatattct ccagcttgtt ttccattagg
     5521 aactggataa tgcggaagta atttctgatg gaactggaat tcaaatgaaa tcgattcagc
     5581 taaacgctgg gcgcctatta cagcttggga aagacccgct ttttcgaatc gtttgatcat
     5641 ttcttgttct gaaagaagat agacatttgc tgttagtggt aaagatgcag ataaattttc
     5701 taaaggtatt tggcttccct ctttgatatg tttcatcact tcaagtgcaa aaagttcttc
     5761 ttgttgtaaa ctagcgatgg gatgtaatgc acaaagcgaa taattttctt ttatcacata
     5821 ctgataccat tcaggcaatt gatcaggcgt gttttctgtc aagccgatat aaaaatttct
     5881 agtaaaaaca ttttcaagta aaaactgcca atgaccgaaa aagttatcct ctttattgaa
     5941 agcctcagaa acagaatgat ttgccggaac aacaaacgct agatggtcgg tttctgtcat
     6001 ttctgcaagt tccaattttt cttggatcat ttttcggctg gaaagtttca tcagctgctg
     6061 ataacctatt tcatcttttg cgtacgcaag caagccataa tttttttctt cataagtata
     6121 actaagatgt aatccaatga ttggctgtac tccttcttct acacaaatac gatagaattc
     6181 aggtgcaccg tataagacat ctttatccgt tatggctaat gaagtgtagc cttgtttttt
     6241 tgcttcttgt acatattctc gaatacgtat cgtactttgt aaaagagaat aagaagtaat
     6301 tgtataaagt tgtgggaaaa acatcttgca ttccgccttt tttttcgtta aaattcacta
     6361 gcgttaaaag gtttgatcct tttactttca ttaagatagc aggcgctgaa gttgattatt
     6421 ccacttcatt tgcttgcttg taatctgtag gaatgagttc tttcgcaaga actcaaattt
     6481 tattgagatt tggtctcgtt tctttacatt caaagaaaat ctggtaaaat taagatagaa
     6541 aagaggtgtt ctttatgcga tatatcgtta cattattctg ggctgtcgtt ctaggtcaag
     6601 tagtcggtta tattggtgct gcattaacaa gcggaacata tgattttact ttgacaacaa
     6661 tcatttcatt cattgcgggt gtcattatcc ttttgattgg tgcagttgcg ccaagaaaag
     6721 aaacctctgc ccattcgtaa aaaaacagcc aagagcttga gatttctcaa gctctttttt
     6781 attttctact cctcaccacc attataatct gctgcgaaga actttacgaa agttgaataa
     6841 agaaagaaga atatttgaca gaacaaacaa acttgtcaca ataaaaacag cactgtaatt
     6901 ataagcatgc gcgactgtcg atcctagcat gggacctaaa acttgtccaa agttgctaaa
     6961 catctgattg tagctataga ttcggctgac tccttcttga ggcgtaatct tactgatcag
     7021 tgtattgaca gaaggcatca aagcgccagt tgaaaagcct aaaataaagc gaagtatacc
     7081 taattggagc ggtgtagtga cgaatgccat aggtaaataa cagcaaaatg ataaaatgag
     7141 tcctccaatt agaacttttt gattcccaat cttatcccct agttttccta gtttcggtga
     7201 agaaaagaca gcggaaacac cagctatcga aacaatcaag ccgctgacaa aaaggatatt
     7261 ttctgtactt ccgctcaatt cacgaatata taaggtcaaa atagggctga tcgtagtgat
     7321 tcctatctgt aaaatcaagg tcgtaatgaa gagaccgatc aaaatagaga gatgatccat
     7381 ttttgaaaaa atttctttgg tgcttatcag atcttttttc tcgattggat ggaaatcttc
     7441 tttgactaag aaaatggtca ataccgttgt gatcatcaac aaagcccctg tgatcaagaa
     7501 tacattttcc ataccaaacc actgagccag tgcaccgccc attgaaggtc cgatcagatt
     7561 tccagcaatc gctccggtag acaatgttcc taacgcccat ccgctctttt ctctaggtgc
     7621 ctgtgaagca atcatcgctg tggcatttgg aatatatcca gacaaaatcc cattccaaaa
     7681 ccgcatgatc aaaagccaat acgcgtttgg tacaaaagcc aaagatccca ttgttaatgt
     7741 catccctgct gctgcacgga tcatcatgag ttttcggcct tttcgatcag ctagatttcc
     7801 ccagattggt gcaaccacgg cagatgcaaa agcagtcaca gagattgcta gtcccgaaaa
     7861 caattcgatt tgatcttttg gtgttcctag ttgctcaaca taaactggta tgaatggcat
     7921 aactaaacta atacttgcac cagtgaaaaa acagccaatc cacgcaatca ctaaattttt
     7981 tctccaatta atctccatct atcatccctc tttcgtctct tttatcatac ctattttcga
     8041 ataaacaagc aacgatctcg ccagtaaaaa gagaaaaatc ccgcaaaggt cattttatat
     8101 gtaacgttct ttttatgtaa aacaaaaaga gatcagaaca aatactgttc caatcccttc
     8161 tctttagtta aattgttgct agaatgatga agttgataat gaataaaata tcaacgatcc
     8221 acaaagcaac agaaacttga ttgtattttc ctttgattac tttcacaatt gtatagaaaa
     8281 taaatcctgc tgcaatccca taggaaatgc tatagcacaa tcccataaag atcgatgcaa
     8341 agaaggccgg taaagcttct tccatatcta gccatttgat gtctgcaaaa gaagccatca
     8401 tcattactcc aactaaaatc aatgctggcg cagttgcttg atttggtaca atggcgatca
     8461 gtggagagaa taaactactt aatgcgaata gcactgccac tactactgaa gtcaaaccag
     8521 tacgtccacc ggccccgatc ccagctgcag attcgacata agttgttgta ttcgatgtac
     8581 caaagatagc ccccacagaa gtagcgatcg catcggcaaa tagtgcttta tccatttttg
     8641 ttttgaaccc acgaccatct tccaacgcga tctcgtcttc ttttgagaaa atacctgtgc
     8701 ggcgtcctgt accgataaat gttccgatcg tatcgaaagt atcagataaa ctgaatgcga
     8761 tgatcgtcat caatacttga ggaatcttgg aagaatcact gaataaagat tgcatgcctt
     8821 cagaaccaaa tgctgcaccg aaagttgttc ctaattcttt aaatgaatta cctaacgaat
     8881 tcgtttgcca atcaatggaa cttaaatcaa caacacccat aaaaatacca aggactgtgg
     8941 tcgctacgat accgataaga atagccccac ggacattttt tacaactaaa atggctgtca
     9001 gtaccaaacc aatcacagcc aataagatag gagcgttgtc aaagttggct aacgcaggga
     9061 cgattccacc atttatcgta acatttgtcg cttttccgcc ttcaacgaca gagctcgaaa
     9121 tagctgactg gtctgctgaa aaagataaga atcctgcatt tttgattccg acatacgcaa
     9181 cgaaaatacc gatcccgcca ccaatcgcat gttgaagact ttcgggaatc gcccgaataa
     9241 tcatttttcg aatatttgtg acagtaataa aaatattgat taatccgcaa ataaacacca
     9301 ttgccaaggc ttgttgccaa gaataaccaa gcccaaaaac gaccgtaaaa gtgaaaaatg
     9361 cgttcaatcc cataccaggt gcctgagcat atggtacatt tgcgaacaat cccattacca
     9421 gcgtaccaat aatcgatgca atgattgtcg ctagaaatac tgcttggaag ggcatacctg
     9481 atgctgacaa aatcgtcgga tttacgaata agatataact catggcaaag aatgtcgtta
     9541 ctcccgccat tatttcagtt gatacatttg tattgttttt ctttaaaccg aaaaactttt
     9601 ccataaaaag ttccactccc tgaatttttt tctgatggaa aactataata ataaaaaata
     9661 agtaaaatag ggcaaatcca tcgaacaatt tcaaattata tagacgtttc attcgctttt
     9721 caacgttttt ttaacaaaaa aagcgaaaat aatacttaac gttcgtgttt agcttatgtt
     9781 tagctattta tatagccata actgtagttt ttgttaggaa tagctgattg ctcttgttaa
     9841 ttacctaaaa tatgctagac taaaacaaat gctagttgag gaaggaagga cccttattga
     9901 ataaaaaatt aattgccatc gatctggacg gaactaccct caatcaagat tcgatgatta
     9961 catctaaaac aaaagagact ctaaaaaaag caatcaatgc cggtcatcat gtcagtattg
    10021 ctacaggacg cccattccga atgagccatc agttttacca acaattagaa ttaacaacac
    10081 cgatggttaa ttttaacggc gcattagtac acatccctaa tcagcactgg gatggtgaaa
    10141 gagaaacgtt gatcaatcgt gaaatcgctt ttgaaatctt atcgcaaaaa aagcagctaa
    10201 atttagattt tatcgctgct gaaaatcgtg atactttttt tattgacagt tttgatttct
    10261 ttgatgaaaa aatttttgct tcttctcgtc caggagagaa gaatttgctt tctcctaaaa
    10321 atttgacgac aaatcctaca tctttgcttg tccgaacaga caaacgtttt gcaggagctg
    10381 tatctgctga attaacacgt caatttggca gttatgtcga tgttcggaca tggggtggac
    10441 ctactgccat cttagagatt gtttcaaaag gaatccaaaa agcaaaaggc gtgcaagaga
    10501 ttgctaacta cctgtctatc gatcaaaaag acgtcattgc ctttggtgac gagcataatg
    10561 atttggaatt gttggattat gcaggttggg gagtagctat ggctaacggt actgaccaac
    10621 tcaaaggcat tgcaaatgat gtcactcctt tgagcaatca ggaagacggt ttagctgttt
    10681 atctggaaaa actacttaaa ctataataga gaattagatt ccacaaaaaa gaggttgtga
    10741 caatttttgt cacaaccttt tttgccgagt aaacggtgtt caaaagccag tttctcggta
    10801 tgaagtcaaa tttaagcaaa aatgtgagaa gctattttca caaaattttt cttcatactc
    10861 gaaactaagc ggctttttca cgacctcttt tttattttgc catgaacata tcataatacg
    10921 cgtccattat ttctttagca atcgtcaagt ttatgtggtc ttgttcgtct aataagttcg
    10981 gcaagacaac actgatggca atttccggat tgtctgttgg tccgtatgcc acgatattac
    11041 tattgactgt cgcaatatct ggatgacctt ccgcaaccgt ttctgcagtc cctgtttttg
    11101 cggaaaggtc cattttcgca ctggctaatg gtttggctgt tgtgtaagga tcggtaccat
    11161 ggacaacttg gtggaatcct tcacggagga tcgccatatt ttctgcagaa atattgaccg
    11221 tattttctac tgttgtgcca atttctttct tcacttcacc caagtttcca ttttcgtcat
    11281 tgccatagat cgctttgact aggtgcggct gtactcgttt cccgccatta gcgactgttg
    11341 aagcatattg agctaattgg attggtgtat atgtgtcaaa ctgtccaaat gccaaatcgg
    11401 taaagttacc tggagcatac cattctttgc cattatccgc attgaacttc ttcatataat
    11461 caacagaacg tgagatccca ggagattcat tcggcaaatc gatcccagtc gttgtaccaa
    11521 gaccaaactc tttgaaagag tctctcaatt gctgataagc tttagcttct tcatctaaac
    11581 taggtagtcc cattccaggt gtatagtcta gtcctaacat ctttaacgcc actttgatca
    11641 tatacgtatt agaagacaat tccgatgctt tgactgcatc aagagcaact tgtccgcttc
    11701 gattaaagac agagcttttt tctgaagcac cttgcagacg aatcggctca tcaatcaaaa
    11761 cttgatttcc gctgatcgcg ttacttgccc agcctgctgt caaagtacca gccttgacta
    11821 cagaccctgg atcaaaggcg ctagtaatcg tgcctaaagc attctcggtg atttctttgg
    11881 aaccttgttc atgactgaaa ccagtcattg ctagcacttc tcctgtctga ggattcatag
    11941 ccactgcata agcaccaggc gaatactgtg ctttgccgtt gttgatcaat gtttgataat
    12001 tccttttcaa aatttcttct acttttgatt ggaattctgc attcatagat aacattaaat
    12061 tagaaccttt ttccccagag aagatttctt tttgattcga aacatttcct tcattatcaa
    12121 gggaaacttc atattgggtt ttggttcctt gcaggacatc ttcatattgt ttttccaaat
    12181 aactttgtcc tacacggtca ttccgagaat aaccttttgc taggtatttc tctgcttcat
    12241 ctgctggaag tccttgcttt tcagtcgtca cactgccaag aatacttttc agtgaacttg
    12301 ctgcattata ttctcgtgtc cagtctgtgc cagtcgaaac tcctggaagt tcagaagcgc
    12361 gttctgccac aacagctaac tcttgatcag taacaccaga gtttttgata tagacagtgt
    12421 ttaattcata tgcactattc atccgtttaa agatggtagc aatttttaat tgctcctcgt
    12481 ttaaattaat ctcatcagct gtgactttat tgaccatcgc actatattgt tcgctgttcc
    12541 ctaattgctt ttcttttgcc gaaagtcttt ttgtggcttc tttcaaatgt ttgggatcag
    12601 ccagccaaaa atctttttta tctctatctg tcagattttc atctaccgga acatcgatca
    12661 gcgagttcag cttcgttgcg atttcaagca aatctttagc cgtaacttgt gttcctctcg
    12721 tatatgtgat ggcctgattc gctttattcg taactaaagc ttttcctgtg gagtcgtaga
    12781 tcattccgcg aggagtactt tcttggaccg taatcgtaga agtagacttc actcgcgcct
    12841 ccatctcttc gccgttcacg atctgcaaat aacccaatct agcaatcaac gcaacaaata
    12901 acgtaaatat aatgaaaaaa agaaaattta agcgaaaagg aacatgggac cggcgaaacg
    12961 ttttgttctt aggttcttgt gcaccttttc ttattttttc taaccaagaa gtatttaata
    13021 tttttttcat aaaatctttc atcatttcct atccttccgc ctatttacat tcatgcttca
    13081 ttgtacccaa aaacttctta ataaacaaga agtatttaat atttttttca taaaatcttt
    13141 catcatttcc tatccttccg cctatttaca ttcatgcttc attgtaccca aaaacttctt
    13201 aataaaaaag aaaaaatggg cggagcaaga aattctttac ctaatttcaa gataaaatca
    13261 actttttgtt tcacagcaat cacttaaagt tacatatcca actatttttt tcattaaaaa
    13321 ataaacttat ttttgaaaat tatgttttct taagtcgtga aacactttga tggatcattt
    13381 atttgtggaa aactacgttt tgttattcta aaaaacttat ataaaaaata gtaaggtttt
    13441 tatttcgaat ccataaaata ttcttggatt gcttctcgtg tttttttcac tttttcgcta
    13501 agcttttcgt ttttagccat atagaaataa aaaatgatat cgatcgtagc aaattgggcc
    13561 aaaagtgaat tagttgccgc acttcgattc gtgatttctt ttggtctaga tgtttgtacg
    13621 agtacatcgg ctatcttgct aagcgggtta tttccaaatt gcgtcaacgc aatgatttcg
    13681 atattcatgc ttttcgccag ctcggctaat gcgactacat ctgcacttct acctgagttt
    13741 gaaacaagcc aaagcacttt ctttttctca ttgctgacaa gttgcgggag caagacgtgg
    13801 atatcttttt caaaaatgat tggcttaccg acacgagacc atttttgcag aatatcttct
    13861 actactaaag aagaggcgcc aaccccatat aaaaaaacag tctctgccga ttccaaacac
    13921 tctacgactt tttggatcgt ttcttcttct agaatatcta ctgtttcttt tatcgctact
    13981 tgtgcattgt taccaagttt gtttttgata gagtaaaagg actcatttgc ttctatatca
    14041 ctaaaacctt catcgattcc agtctgtagt tctgttgaaa tcgtgatttt gaagtccgtc
    14101 agactttgaa aaccgatttt tttggaaaaa cgtacaactg tcggcgcact caacccggaa
    14161 gcttctgcta attcattagc tgtcattgta gggaccttct caatattacc gataatgtag
    14221 ttggccaact ttttttctgc tgaagaataa tcttccatca tactttcaat acgactaatt
    14281 acgctcataa gttcctccac ccttttaatg atagtctatg taagtatacc atatcagacc
    14341 cttattcacg gtcgtatact ggataaaaaa caaaaaaaca atccgcccaa cagaatatct
    14401 tatcccatca atcccacaga ttctattgag catctgtgtc tttgaggaat acctgttatc
    14461 tattgttcgg attgctttct atctactact tttttacagg aaatttacct attagttttc
    14521 tctgctgtaa tttttgactt ctgcttccat agcatcaata aagatattca aatcttctac
    14581 agctgtttct ttacttccat aacgacgtac gttcactgtt gcatcttcca attccttgtc
    14641 tccaacaact aattggtaag ggatcttttg tgtttgagag gcacgtatct tatagcccat
    14701 tttttcatta cgatcatcaa cttctacacg caaccccttc atttgcaaac gttctttgat
    14761 ttcataagca taatcgccat gtgcatcaac agaaacaggg atgattgttg cttgtatagg
    14821 tgccaaccat gttggaaaag cgcctttgta tacttctgtt aaataagcga caaaacgttc
    14881 cattgtagaa acaattccgc ggtggataac tactggacga tgcgtatttt ccccgtcttc
    14941 tccaacatat gtcaaatcaa agcgctctgg caataagaag tctaattgta tagttgataa
    15001 tgtttcttct gtaccaagag ctgttttgac ttgaacatct agttttggtc cgtaaaaggc
    15061 tgcctcgcct tctgcttcaa agtaatctag ttctaattca tccatagcgg ctttcagcat
    15121 tgcttgggct ttttcccaca ttgcgtcatc atcaaaatat ttatctgtat tgtttggatc
    15181 gcgatagctc aagcggaaac gataatctgt aatattgaag tcagcatata ctgccaccat
    15241 caattcaagc gtacgtttaa attcatcttt gatttgatca ggacgaacaa atgtatgacc
    15301 atcattcaac gtcatttctc gtacacgttg taaacctgac aaagcacctg atttttcata
    15361 gcgatgcatc attcctaatt cagcgatacg aattggtaat tcacgataag aatggatcgt
    15421 gtttttgtag accatcatgt gatgagggca gttcattggg cgaagaacaa gcatttcgcc
    15481 atctcccata tccattggcg ggaacatgtc ttcttgataa tgatcccagt gtccagatgt
    15541 tttgtacaac tcaacatctc ccattattgg tgtatataca tgttggtaac ctagactaac
    15601 ttctttgtct acgatataac gttcgatcgt gcgtcggatt gttgcccctt ttggaagcca
    15661 gaatggcaaa ccagaaccta cttctgggga aaccatgaac aagtcaagtt cttttcctag
    15721 tttacgatgg tctctttctt ttgcttcttc acggagacgg atatattctt tcaaagcttt
    15781 cttgtcaaaa aaggctgtcc cgtatacacg ttgcatcatt tgattgtttg agtttccacg
    15841 ccaataagca cctgccactg ataataattt aaatacttgt atacggccag tggatggaac
    15901 atgtggtcca cgacacaaat cgatgaattc gccttgttga taagcagtaa tcacttcttc
    15961 ttctggcaat tcactaatca gttctacttt gtacggatca cttgcaaaaa tttctaatgc
    16021 ttcctgtttt gaaagaacac ggcgttcgat cggcagattt tctttgacga ttttcatcat
    16081 ttctgcttcg attgccggca aatcttctgc agttacttgg ttttgtccat tatctgtatc
    16141 gtagtaaaac cctgaatcga tagctggtcc aacgccaaag tggatatttg gatagaggcg
    16201 acgcatggct tgggccatca aatgagctgc tgaatgacgg actaagccaa gtgcatcttc
    16261 atggtctggt gtgatgatct caatacttgc atcttcctcg attggacgag tcaaatcaac
    16321 gagttttccg ttcactttac cagctaaagc tttttttgct aagctttttg aaatgctttc
    16381 agcaattgcc aatgttgttg ttccagactc aaattctttg actgcaccat ctggaaaagt
    16441 aatttttatc ataatagttt cctccttgaa atgttttgat tgatcagatc atcaagctga
    16501 aaaacaaaaa aatcctagca tctcttgaaa agagatacta ggacgaaaag ttcgtggttc
    16561 cacctaatgt tcaaacgcaa atgcgttcct caagcgttgt aacggtccga ccgcccttgc
    16621 ttcaacaagg gtttcgaaag tggtcatcct ttggttttct cctggaaaag tttcagccta
    16681 gcttttcctc tcttatcgaa gactctcaaa ttcagttggc tttctcatca atcaattatg
    16741 tgtttattta accactaata aatagaaaaa tcaagtggga aactaaactt attttttcca
    16801 ttccccattt acaccgtatg ttcctttttt catctcattg atgatgacat ggatgtgttc
    16861 ttttggtgcc tgcgtgtttt tatgaacagc ttccgtgata tctttcatca tattttctaa
    16921 ttgttccgga ctgcgacctt ctactaattc gacatgtacg aatggcataa tgctctctcc
    16981 tctttctgat cgttcttttt tcaactcgat tctatcatac tatgtttgtc ttgggtactg
    17041 aatcaggaaa aatggctgga tctttttttg ctctcaaggc actcaaaata gagaccgtat
    17101 ccacgacttc ttgcaaaaca gctccgaata atgcggggat tagtcccgta cttgcaatta
    17161 acatcaaaaa aatacaaata aagatgccaa tcagtacaga ctgtttcgct attctcatcg
    17221 tatctcttga aatcactact gcttttgcta cacgcaagag atcatctttc aagatgaccg
    17281 catctgcact ttcactagca gctgtagaac catgagcccc cattgcaata ccgatatctg
    17341 cagcagcaag tgctggtgcg tcattcacgc cgtctcctac catcgcaaca ggtctttgat
    17401 ctttagggat actttctaaa atggtcagtt tatcttgagg caagctacgg gcatgtacag
    17461 aatctaattg gactttttct gcaatcgttt gagcaatggc agatccatcc ccggtgatca
    17521 tcatcaaatt tttcacatca agttttctta gctccttgat tgtctttgct gcttctgggc
    17581 gaatctgatc cgaaaatagg atcgttcctg caaaagatcg atcaattgtt acatagattg
    17641 ctgtattgga cgtttctatt tcttcagctg aaacaaaaga ggcacgtcca atctttactt
    17701 catgattatt gactactgct tggattcctt gaccggtcac ttctttcagt tgactaacag
    17761 gatacagttg gctgtttttc acgttgttca ctaacgaacg agctaagata tggctagatt
    17821 cctgttctgc actagctgca tacagaagta gctcttcttt ttttattcca ttgactggct
    17881 gaatcgcctc tacttccaat atccctttgg taagcgttcc tgttttatca aaagcaatcg
    17941 tttttgtacg tgcgagcttt tcaatcgttg ttccagtttt tacaacgatc ccgtttttac
    18001 ttgaacggct cattcctgca actaaagcaa tgggcgcagc tagaatcagt ggacaaggtg
    18061 atgccactac cagtacctca gcaaatcgta aagggtcttt tgagaaccac caagaaatac
    18121 caccgatcaa ataagcaatc aacgtgaaag ggactgcata acgatcagct aaacggacaa
    18181 atcgggcagg cttttctttt gattcttcga ctagtttaat aatcatttga tactggctgt
    18241 ctgcggcacg tttttcagcg atcatttgga tcgatccgtc tccatttaaa gaccctgaca
    18301 taatctgatc tccaggtttc ttggtttccg ggcgggactc acctgtcaaa gaagattcat
    18361 cgaccgaaga ttctcctttg gtcactcgac catctgcggg tacaagttcg ccaggcttga
    18421 ctagtaactg gtcatcaata tttatttcat cgattactat atcctgcaat tttccttctt
    18481 tgaatcgatg ggcaattctt ggggtgttat ctaacaaaga ctgcagttct ttacccgctc
    18541 gtttattcgc gtaatcctct agactatctc cgcccgtaag catgatcaat atcatcaaac
    18601 tagcccagta ttctttgacg gctaatgttg caaggattgc ggtaatcgct aatacatcta
    18661 cgccatatct tcctgaacgt aacgtcttga tcatttccaa aaacatcaag aaggcgagaa
    18721 tccctccagt aaatgcgaca atcgcaaatg caagggccgg ctggtgaaaa acaaattcac
    18781 ttaataaagc tatcccacca ataagcaaag taagtaaaaa ttttttgaaa tgtgtcattg
    18841 ttttttctcc tcttttatgt gacttagaga ggttagtaat aaatttccag ctgcctcttt
    18901 catcttaagc atagtccttg actagcactt tttaaaacaa taggccttaa ttgaaaacaa
    18961 ctatcactat cagcgtttcc aaaattcatc agttctacaa acaaaaatat tcagcaagga
    19021 ttactttttc gattcatcag ttaaaaagta ccttttttcg aaaaactatg ctatactttt
    19081 aattcgttat ttacggcgga aggagcaaac atgcgatgaa tgagaaacaa ctagaacaaa
    19141 cgcacgtgga tgaaacgatc gaactaatcc agatcgaaca aaatatgctg gatacaaaac
    19201 aacaagaatt gactcaggaa atgaatcatt cgacaaaaga cacagctaat cacaagattc
    19261 gagctggttc caatgaagct ttttatgaat ctgctgttga atatcgccaa catgagcagg
    19321 aattgctttt gaaataccat actcttgaag cacagcaaaa acgcctgcaa acgttgtctg
    19381 tcatgaaaga tagtccttat tttgcccgta tcgactttaa agaagaccgt gaaacagaaa
    19441 cattatatct aggtatcgct tctttaagag atctggcaga agaaacaatc gttattgact
    19501 ggcgcgcacc aattgccaat ctttattatg aaggtgagat tggtcccgca ttttatgaaa
    19561 ctgaaacaga tagaatagat gtcgatctgc agttgaaacg acaatttaaa atccaagaag
    19621 gacgtatttt atctatggta gatacgtcgg aagtaatcaa tgatgacttt ttgctagaga
    19681 tattggatga atcatcaagc aaccatatga aaaacatcgt ctctacgata caaaaagcac
    19741 aaaatgcaat cattcgggac acaacaagta aagtaatgct gattgaagga atcgccggaa
    19801 gtggaaagac atccgctttg cttcaacgta ttgcttttct gttgtatcat aatcgcaagt
    19861 ggctcgaggc tgaaaacgtt cttctatttt cacccaacca tttgttttct gattacatct
    19921 cgactgtatt gccttcatta ggtgaaagcg gcgtgcctac acagacattc aaaaattatt
    19981 tggtgcagct gctgccagag ttttctttac tagatgaaca gcaacaagaa agtggttttt
    20041 tgctagggga aaaagatccg atacaagtga tgaaaagcgg attgacctta gtcgatcaga
    20101 ttgatcgcta tatccaaggc atcacaaact acggtccctt gttccgtgat atgaaaataa
    20161 atggccgtac gatcctatca aaggaatcga ttcgccaatg gtataaagaa acaaatgaac
    20221 aattaccttt acatcataga ctttctctat tacaaacgaa gctcctgaaa aaattaggcg
    20281 gtctgcaaaa agacgaaaca cgtcagcaat gggtcaaaga tctggcggaa gaacagctcc
    20341 aagaactata cgcaacagat ccaaatcttg aatatacaga acaaaaggag cgttcattac
    20401 gtaaaaaact ggccaaccaa atcgtacgta aaagattccg tcgaatccat cgagggatca
    20461 tgaattatca atttgtcaat ataacgaaac aatatctcca ctttctacaa actgtgccac
    20521 tttctcttct aacacgacat aaaatcagcg agaccaggtg gcaggaacat ttgacaactg
    20581 ttcgtacaca cttgcgtcaa agagaattgc gtcaagagga tgctgtacta ttctttttac
    20641 tgatgcgccg aacatatcca gtgacggtta cacacaaagc tcgctatatc tttattgatg
    20701 aaatgcagga tttcgcacca tcacaagtgg cattgctgca agccttgttc ccaaaagcaa
    20761 atttgacttt ctgtggagat ctaaatcaaa atgtctttgg aaatgaaacg atcgttggat
    20821 cattggaaga ccttttcgtg gaccgtgaag tcactcgttt ccaattaacg acaagctatc
    20881 gctccacaaa agaaatcact gattttgcta atcattttat tgtgaaagaa catcaagtag
    20941 aaacaacagc aagaaaagga gaccttcctt tgctcatcca aggaacaaca gatgatatca
    21001 aattgaattg gttagcagat accattcgaa cgactcaggg aaaagcccgg tattggcgta
    21061 cagcaatcat agctaaaaca caagaagaat gcgaagctct ttatgctcaa ctgccagacg
    21121 agttgaaaca acaagtccag ctgattttaa atgagagtga ctttatgaag cgttccatta
    21181 ttttacttcc agccttttta gcaaaaggtc tggagtttga ccgagttttc ttgtgggatg
    21241 tagatgcaga acatttttct tctcagcaag acaaattgat cctttacacc atgtgtacac
    21301 gtgcgatgca tgaattgacc ctttttacct ccaataatga aagtcctttg ttaaacaata
    21361 tggatatttc atcttatgag aaaaaagtac tgtaagtaaa aagacaactg ctatcattaa
    21421 tgttagcagt tgtcttttta tttacaataa taattgcaga aaaagtaatt caatcattta
    21481 aggtcattta ttctatatat aaatccaaat ctgtttcagg atattttaat ttgacaagcg
    21541 aaataatcat tttcagttct tccatgtttt ctggatattc tgggtcaaat accttgctat
    21601 gacggccaat cccgccattg tcgatcaatc ggtaaattcg attccattca atgaattggt
    21661 caattgattg gaaattgctg attggatagc cgccacaatc caaaatctct gatacacgaa
    21721 atgctaggta tgcactatcg atcactttat tttttttcgt gtctatcttc tcatttcttt
    21781 cgataagaat tttttcagga cgatgtttca agctccactc taatagtaga aagaaagcct
    21841 ccatccgata ctcttcttca aataaccgaa aggctgaatc agaacgacga aaaagcagcg
    21901 gaatcagata attttgtcta taaatcagca ttggtgcgcg gtccaccata cactctaaat
    21961 aacggttcag ataccctgta ccgacatagt ttcctaatcg atagccttca acgccgaatc
    22021 ttgcgtattt ttgcgctaat tttgaacaat cagttacgtt cacagctaac tcttctactt
    22081 tgcggtaacc gcttaagata cgttcaaaag gttctaaagt gatttttgct tccatattct
    22141 tccccgtcca tcttccacat acttcatatc atttcacatg atacttatgc tctcatcata
    22201 ccacaggttg aattagaaaa gaaaataaat cacacaaaaa agcaagacaa tctgttagtg
    22261 agattgtctt gcttttcgaa aaagactgaa tacttttcgt taaaactggt tgcttagtct
    22321 tctaatgcca cattatggaa cacatgctgt acatcttcta gatcttccag tgcatcaatc
    22381 attttttcaa atgtttcttg atcttctgga gatagtgtta cctcgttttg agggatcatt
    22441 tggatctcag cgattgaaaa atcagtgatt cctttttctt ttaatgcttc ttgtatacta
    22501 tggaaatccg ctacttcacc gtaaacgatg atttgtcctt cttcttccat tacatcgcga
    22561 acatcgatgt ctttttccat taggtattca aggatctcgt cagcgtcttc tcctgcaaaa
    22621 gcaaagattg ctgtattgtc aaacatgtaa gaaaccgctc cggaaacacc catgttcccg
    22681 ccatttttcc caaatgctgc ccgtacgtct gctgctgttc gattcacatt gttcgtcaat
    22741 gtatcaacaa tcaccattga gccgcttggt ccaaatcctt catagcgcaa ttcagagtat
    22801 gtttcatctc ctgcaccttt tgctttttcg attgctcggt caataatatg ttttggtaca
    22861 ttataagttt tagcgcgatc aatgacgaaa cgtagttttt ggtttgcatg cggatcagga
    22921 tcacctgatt ttgctgccgc gtagatttca atcccaaatt ttgcataaat ccggctatta
    22981 ttagcatctt tagcagtttt ctttgctacg atatttgccc atttacgtcc catattttca
    23041 cttcgctttc tgatttgata gtttcataac gcaaaaacgt cctgttccat tatacttgtg
    23101 aatcacgatt ctgtgaagct ttttttcttt cttccttcat tcttctcatt tttagtcaat
    23161 tatctatact cggattggga tttgcccgaa attctgttcc ggaatattgc ctcccaattg
    23221 atagatttga tcacttctaa ctaacagttc cgcttcttgt gcctcttttt ttccgattcg
    23281 ggaataaata gaaatctctt ttttttcttt taagaatctt cttccagcat cggtaaatcc
    23341 aagtagtcga agataatttt tattttgttg gacaggaatc tcttgctttt tgatattcag
    23401 caagacataa caagataatc gttggatccg tgtccatgta tatctttttg tttttacctt
    23461 tgacacaaaa tcctcaaaac tatcagcagt tgcagctgct gttttcatgc gcgattctat
    23521 tccttcggtc atctgataga tatctgtcag ttccagcaaa gtagaagata acaaccgata
    23581 acgcaaatac ggccaataag attcccaagt ctgcagcgaa caagttgaaa gatcttcttc
    23641 tgtctgaggc ggcacaagtc cagctattct ttttccttct tctaacgcat ggcgaatggc
    23701 tgttgcactg gctatcctct gatctgtgat gattttttca tgatagcctg cacttttacg
    23761 tcggatcgga tgtaattcca tagggtggac atacttggca ttttcctttg cataacttaa
    23821 tcccaagata tgatttggcg aagaaaaatc aagcgtaata tcgggataca gactgcgaaa
    23881 aacctcagtc attttttgcg gataactata cgtttgatca gacagttctt gaaatgcctc
    23941 ttctatccta tcttgattct cttggacaaa gcgtccgaaa ctgctgtaat caaacgaagt
    24001 ctcttcttct gttccaaaac acaaactgtc acagtccagt gtttgcaaga tttttactgc
    24061 acctttagca aaatagtcag ctgactgaac cgcccattcg acgggaagct ccactactaa
    24121 atccacacca ttttgtaaag cttcattcgc gcgatgccat ttatcgatga tagccggttc
    24181 gcctctttgc aaaaaattcc cgctcattac agcaatgacg acctctgctc ctgttatttt
    24241 tcgtgccatt tccacatgat agcgatgtcc attgtgaaaa ggattgtact ctacgataat
    24301 cccgcagctt ttcatgcgca tcctccttca tagttatcga taaaaaagaa atagagacaa
    24361 gtaaaaaaag gcaaaaatcg gaaaaaatac ccgaactata taaactattc tcgcaaacac
    24421 aatacttctg cgacgaaaag ctgcactgca agagcaatgc ctatatcgtt cggatatata
    24481 atttatgttg aagatttatt cttttcttgt taactcgagc aggcaaaacc gctagtcttt
    24541 gtatgctaca aagaaccagc gctttgattt ctctgatgct gcttcgtctg taaaatcaga
    24601 aaatgcttcg actttcgaaa agccgctgtt ttccagcatt cttaaataac tttccaatgg
    24661 ataggtacgc tcttgatgta gttcatcttc acgaataaat cgaccagacc cttcttgttc
    24721 ttcaacgaaa aaggtaagaa aatgttcgat gctgtgtttc tcgcttcctg gataactatc
    24781 ccacaaaaaa gcaaaattat ccccttgata atgatagcta tattctggaa aaacctcatc
    24841 tacttgatag gtggagtgta cgtcaaataa aaatcggcca ttttcttcta acgcttgata
    24901 gacttcatca aacacttgct gtacctcttg tctatttttc atatagcaca gcgaatcaga
    24961 aaaacaggta attgcctgat attgtccgat ttctgataaa tcgagcatat tgccttccac
    25021 aaattggatc gatgcgtctt cttcatatgc tcgctggctg gcaatcatca acatctcttc
    25081 tgaaaggtct aaccctgtca ctttatatcc atcttttgca aaggcaaccg ctaatgctcc
    25141 agtaccgcat gccagttcca aaatattttt tgtgtcttct tgtaaatgac gtttcgaaaa
    25201 agctaaccat tgctgataaa gtgtttcgtc catcacttca tcgtaaatga aagcaaatgt
    25261 ttcataagcc atatgttaat ccaaccattc actaagatcg actagcggag catctgacca
    25321 tagtttttcc agattataga aatttcgttc tggcgcatgg aaaacatgta cgatcacatc
    25381 tccaagatcg atcaataccc attttccgcc ttcttttcct tcaatacgtt ttacttcata
    25441 tttgttttct tcttctttat caatgatttc ttctgtaatt gcgttgattt gacgttcgct
    25501 atttgctgaa cagatcatga aataatctgc caacaaggaa acatttcgaa cgtctaaagc
    25561 gataatgtct tctgcccgtt ttgaatctgc tgcttcaatc gcaattttta attgttcttt
    25621 actatctatg atgatttcct cctaatatga gcagcttatt tttttgctac ccagtaatta
    25681 tatgtttcga ttgtttttgg ataaatctgt tgttcttgcg caagcaaatg ggccaatgta
    25741 tgttttgttt caaatgctac agcttcgtct agatcagccc atgcaatggc tcgtgcatct
    25801 tgtaccccag gaaaatctcg acctggttca atatagtctg ctacataaat gattttgtca
    25861 aggagactca tcttagcagc cccagtcgta tgcacccgga ttgcatgaag aatctctgca
    25921 tcggtgattc ctaattcccg ctctacaaac gaagcgccaa caagaccatg ccagattgca
    25981 ttattataat tcaataactc cggatcaaaa ccgtctctta cgattaccat tttgaactcc
    26041 tcatctggtc gttcttttgc ataatcatgt gtcaatgcag caatactagc tttttcagga
    26101 gaagcaccgt attttttggc aagagccacg gctgtttctt ctactccaag cacatgcttg
    26161 aagcgttgtt cgctcattct catctgtatt ttttgcatca ggttttctct agaataagca
    26221 gtatattcat tactatagtc catattcata caacccttca ttttgaatat aatcaattac
    26281 tttatctgga acaaggtagc ggatagaaca gccttctttg atcttttgac gtattttcgt
    26341 ggaactgatg tcgatctcag gtacatctac ccaaattaca ggatatggag tatccgttgt
    26401 gtatcctggt cgacgtatac ccacaaagtt gaccatagaa gtcagctcat cgattttata
    26461 ccattttggc aaatattcta ccatatcccc accgataata aagtaataat ctgtatccgg
    26521 attgttttgc gtcaattcct tcatagtatc ataggtgtaa cttttcccac cacgagctaa
    26581 ttcaatcgtt tcaatctgca aaaaaggatt gtcttccacc gctaattcca acatgttgag
    26641 tcgatgtttt gcatcaatcg tctgtttctc atcaacatga ggtggctgat aagaaggcat
    26701 caaaaatacg cggtcaagac ctaaattccg gccagcttgt tcagccatca ctaaatgtgc
    26761 aaggtgaacg ggattgaatg ttccgcccaa gatccctact tgctttttct tctctaagaa
    26821 ttgaggcatt tcttcaggaa ataattgtga acgaacaaat gtcttagctt gcgtattcat
    26881 catggtcact ccttatattg cttttacttc ttttgatagc ctttgatact tttcttttgt
    26941 ggaaggtttg tataatacaa taacacgtcc aatgatttgg acgatttcgc aatctatttc
    27001 tttttctaat acttgagctg cttcttctgc aatctcatct gtattttgaa gtaacgatac
    27061 tttgatcagt tctctttttt ctaaagcttc ttcaatttga acgatcattg ctgaattgat
    27121 cccacctttt ccgatttgaa aaatcggctg aagatgatgc gctttgctac gtaaaaaacg
    27181 cttttgtttt cctcttaaac tcataatttt ccttcttcct taaatcaatg ctttgcgttt
    27241 caatacatct acaccctttg gcgcccagcc cgcaattact actggcgctg ttacagtgat
    27301 ccagcctaga ccggcaaaaa cgatgtctgt tttttctttc acagaaaact caaaacgaac
    27361 aagctccgga aatgcctcga cttcatcagg acgaggaggc tgtaataatc cacctacatg
    27421 tttttgataa aattcgtctg ctttttccag tttcgttcga tgaatattta catcatttga
    27481 cacataagca ataaatgaag cacgattgcc gctgataaaa tcaaatcttg caagtccgcc
    27541 caagaaaagt gtttgccctt cattcagctg ataaacttta ggtttgattt cttttaacgg
    27601 tgcaatgatc cgcaaatctt ttttacctaa gtagtgcgcc atttgatggc gatggataat
    27661 tcccggtgta tcaatcaaaa aatgcccgtc atctaaagga atctcgattt tatccaaggt
    27721 cgttcctgga aactgcgaag ttgtgattac atcttgtact cccgctgtat ttttgatgat
    27781 ttgattgata agcgtcgatt ttccgacatt tgttactccg acgatataga catcccgtcc
    27841 atcgcggtat tcttcgattt tttctaatag ttccgtcatc tcatgagatt tcttagcact
    27901 agttaataat acatctactg gacgaattcc tgcttcgtaa gcacgttcct tcatccattg
    27961 tgttaattta gtttttttca atgatttagg taatatatcc actttgttcc caactaaaag
    28021 tactggattg tcaccgacaa aacggtgtag tcctgggatt agtgaaccat tgaagtcaaa
    28081 aatatccacc acattgacga tcaatgcatc tttttgccct aattcattca gtaagcgcaa
    28141 aaagtcgtca tccgtcaatg agacatcttg ggtctcatta taatggcgta atctgaaaca
    28201 acgctgacaa tagacttccc cggaagctaa acctttttcc aaagccgttt gcggcgtgta
    28261 tcctaattca ccgggtttgt ttgtttgtat aacggcacca cagccgatac attgtaattc
    28321 ttctgtcatt cgattccacc tcgccagccc atatcagggt gttttgctaa tagataggcc
    28381 ataattcttt tttcccaaaa acgattaaat tgtgtcttcc aactatctgt tgcgataatg
    28441 ggttttacaa ggatgctacg tatccctgca cgattggctc cacgtatatc agtcataatc
    28501 tgatcgccta ccattactat ctcatcatct gctaaatcca attgtttctt tgctatgttg
    28561 atccctcttg ctaaaggttt cattgctcgt gcaacataca aaagatcaaa tttttcaatc
    28621 gctcgcgcga cacgttttga attattgttt gaaacaacaa caacagggat tcctgcattt
    28681 ttcatttcta aaatccaagt aagcaactct tctgttccat ctggattatt ccatgcaatc
    28741 aacgtattgt ctaaatcggt caatactgct ttgatgccta gattctttaa ttgggcaggt
    28801 gtaatctgat agatggcttc taccatccat gttggtttgt aatttgaaaa catgctgtct
    28861 cctcatctgc aaaaaatgaa ggagcacata aagtactcct caaccccgta ttatactgca
    28921 tatcagcaaa aaaaactacc tctatactcg ttcgtctttc agaaaaagca tttctttaca
    28981 aaatgaccaa aaataaagat tgatcacaga aaaatttaga aacaaaaaac aactaatatt
    29041 ggcggaacct cttgtatcgt ttttccagta atttatgctg atctaatcga acaagttccg
    29101 tcaatctttc aatcaacgct tttttcataa tgtgtgcgac tttatatcgt ggcaactgct
    29161 cgccgttgaa cgtttcagga ataactcgat caacgattcc taactcgcgt agttctggcg
    29221 cagtaatttt catcaactca gctgcttctg cggcgcgact accgtctttc cacaaaatcg
    29281 acgcaaatcc ttctggagaa agcacagcat aaatcgtttc ttccagcatc cagacttcat
    29341 ccgctaaacc tagagcaagg gcaccgccac ttccaccttc accaatgatg atggcgatga
    29401 tcggtacctt taaatcagac atttcgagca gatttctggc aatagcctct ccttcgccgc
    29461 gctcttctgc tcctacaccg caatatgcac ctgctgtatt gatcaaggta atcaccggtc
    29521 tattgaactt ttccgcttgt ttcatcagtc gtaaagcttt tcgatagcct tcaggatggg
    29581 gagaaccaaa atttctgcgg atattttcct gcagatcccg tcctttttga ataccgatca
    29641 ctgttaccgg tttgccatct aatcgagcga ttccaccaac aatcgcttcg tcatctgcaa
    29701 ataaacgatc accatggcat tcctcgaaag aatcgaagat ctcttcaaaa aaatccaagc
    29761 ttgtccatcg ttctttagct cttgctaact gaacgacatc atgtgctgtc ttatccacga
    29821 tctatccatc ctttctgtgt atgcaattca agaagcgtat ggattttctg tttcaattcc
    29881 atgcggggaa caatctgatc gacaaaacca tgagataata agaattcagc cttttggaaa
    29941 tcttctggaa gttcctgctt gatcgtctgt tcgatgacgc gtcttccagc aaaaccgatc
    30001 aagctttggg gttctgctag aataatatcg ccttccattg caaaactggc agttactcct
    30061 ccggtcgtcg ggtccgttaa aacagttaga taaaaaagac cttcattgct atgccgtttg
    30121 acagcagcag agatttttgc catctgcatt agagaaaaga ttccttcttg catccgtgcg
    30181 ccacctgaag ctgtgaacaa gacgactgga agactttcct ttgttgctcg ttcgaataat
    30241 ctggtgatct tttctccgac aatcgttccc atacttccca tgataaaatt aggatccatc
    30301 actccaataa tgaagggaat cccctgaatg gttgctttac cagtgaggac tgcttcatga
    30361 agtcctgttt tatcttgcat ttttttaatt ttttccgtat attcgggaaa tgctagcgga
    30421 tctttggtta caagatccgt atcccattct tcaaaacttt tttcatcaat aatcaaagct
    30481 agacgctgcc atgcaccgat tcgaaaagcg tatccgcaat gagggcatac tttttcttca
    30541 ccgatgtctt tcgtataaag gatatgtttg caatttggac actttgccca catgttatca
    30601 ggaacagatg gcgagttagc aggtttgctc tctctattag gattgatccg aatatatttc
    30661 tttttcttaa acagagccat ctatgcgcct cctatctccg tttctggtgt ccaattcggt
    30721 aaaaattctt cttgtaaaaa tgctgtgctg taatcacctg ctatgactgc aggatgacta
    30781 atcaaatcca tctgaaattc tgcgttcgtg atgattcctt ctgtgataaa ttcacttaag
    30841 gctcgctgca tcttcattaa tgcttcaaaa cgcgtatttc catggacgat tatttttgca
    30901 atcattgaat cataatatgg cgggatcgta tatcccgggt aaactgcact gtctactcgc
    30961 agtcccatcc cacctgctgg aagcagtaag ttttggatct tccctggaga aggagcaaaa
    31021 tggaaagcag gattttctgc attaatccgg cattcgattg cgtgtccttg gaatactact
    31081 tcttcttgac acacattcaa aggctcaccg gctgctatct ccacctgttt ttttactaaa
    31141 tcgattcctg tgaccatttc tgttactgga tgttccactt ggattcgagt attcatttcc
    31201 atgaaataaa aatcgccttc ttcatccatc agaaactcga tcgttccggc attttcataa
    31261 ttgactgctt tagctgctcg aacagcagcg tctcctagtg cttctctttt tgtttgtgag
    31321 atcactacag aaggagattc ttccagtact ttttgattgt tgcgttgtaa agaacaatct
    31381 cgctcgccta aatgaatgac atgaccaaat cgatcaccta agatctgaac ttctatatgg
    31441 cgagcaggat aaatgatttt ttccaaatac atatcatcat ttccaaatgc cgctgctgct
    31501 tcttgctgag ctgaagaaaa atgctgtggg agctcttctt ttgattgaac tttccgaatg
    31561 cctttcccgc caccacctgc agctgctttc agcatcactg gataaccaat tctgtctgct
    31621 atttccaatg cttcttctac tgtagataat acaccagtac ttcctggaat gactgggaca
    31681 tttgcttctt gcatcaattg acgcgcattg atcttatttc ccattgcatc gatcgttgca
    31741 cttttaggac caataaatgt gatattgcac tcttcacaca tggaggcaaa tcggctattt
    31801 tcagataaaa aaccaaatcc tggatgaatt gcttcggctt ttgtcacgat tgcagcgctc
    31861 agtacttcct gaacattcag atatgaatca acggcttttg ccgggccaat gcagattgct
    31921 tcatctgcta actgtgtatg aagtgcttcg gcatctgctt ctgaatatat cgctacggtt
    31981 tggatcccta gttcgcggca tgcacgaatg atacgtacag cgatttcacc gcggttagct
    32041 atcaatactt tagaaaacat atttcaccta tccaatcata aaggttaatt ccgcttcggc
    32101 tactttttta ccgtctactt tagcgattcc tttgcctatt ccggctgaag ctttcacttt
    32161 caatatctca acttcaaggt ataaggtatc tcccggtgta actttcttac ggaattttgc
    32221 tttgtccaat ccgccgaaat aagccgtctt tcccttgaat tgttcaagag aaagtaatgc
    32281 aactgctcct gcttgagcca ttgcttcaac aatcaatacc cctggcataa caggttcttc
    32341 tggaaaatgt ccttgaaaaa agggctcatt gatcgttaca tttttcttag caacgatccg
    32401 ttctccttca accatttctt ccactcgatc aatcaaaagc attggataac gatgtggaat
    32461 gatttctttt atttcttgta tgttcattgt tttccctctt tcctgctttg cttatttttg
    32521 ctgaatccta aagagtggct gtccatattc gacaactgac tcgttttcta caaggatttc
    32581 tgtgatcact ccgtcggcct cactagttat ctcattcatc agtttcatcg cttcaataat
    32641 gcagataact tctcctttgg caacttgatc acctactttt ttgaaagctg gtttgtcggg
    32701 cgctggttgc agataaacga ttcccacgat tggcgaaaca acctcgattc catcaaatac
    32761 tgccgattct ttttctttca ttatttctgg agcagttgtt gcttgttcag gtatgacatt
    32821 gactggctcc gaaactggca acgtttcgtt tgtttgaacc atattcatgc ctcgacttgt
    32881 tttattttta ttcatataca attcaaattg accttcacgc aaatcgaatt ccgttagcga
    32941 ggattgatca aattgactga ctaattctct gatttcattg atatccaaaa ttattcctcc
    33001 cagcgtttca tgcacagaac cgcattatgt ccgccaaacc ctaatgaatt actcattgca
    33061 taagtaaaac ttgcaggttt gctttcgttt ttgacaactg tcaagtcaat ctcaggatct
    33121 tgtttttcta cattgatcgt aggtgggata aattgatgtt ctaatgccag caaagttgcc
    33181 aaggcttcga tcccgccggc tgctcccaat aaatgtccag tcatcgattt cgtgctgctg
    33241 acgtaagtat ctctgaaatt ttcacctaat gcgatttgga ttgcttttgc ttctgcctgg
    33301 tcattggctg gtgtacttgt tccgtgagca ttgatatatc cgacttgttc cggctgtaca
    33361 ccagcttctt ccatcgcaag cttcattgct ttagatgcac cagaaccatc tggagtagga
    33421 gctgtcatat ggtaagcgtc acagtttgca ccgtatccaa cgatttctcc atagattttg
    33481 gcaccgcgag cttgtgcatg ttctaaagat tcaagaacta gaacgcctgc tccttcaccc
    33541 atcacaaaac cattccgatt ttcgtcaaac ggaatcgaac cacgattagg atcagttgat
    33601 tctgagagtg ctgttaaagc tgcaaatcca gcaatcccca tctcacaaat cgttgcttct
    33661 gttcctccag caagaatgac atcagaatag ccatgtttga tgttacggaa tgcttcccca
    33721 attgaattcg ttccgctagc acaagctgtt acgatagctg tacaaatacc ttttgcacct
    33781 acacgcaaag caatatttcc agctgccatg ttggcaatcg ccattggtac gaacattgga
    33841 gaaactcgtt ttgcaccttt ttcatgtaga cgaataactt ggttttcgat tgttggaaga
    33901 ccaccgatcc cagatccgat gatgacgcca aaccggtctt gattcatttt ttctttatct
    33961 aaaccgctca tctccattgc ttctaaagca gcgtagattc catagatcga aaacatatcc
    34021 attcgtttgc tatctttttt aataaaatat ttatcgtaag ggaaatcttt gacttcaccc
    34081 gctaaggtaa tccctgtttc tgaagcatca aatttagtga tcggtgcgat gccattcgtc
    34141 ccctctttta agctattcaa aaagctgtct gcgtcatttc caataggtga agtgactcca
    34201 tagccagtaa ttaccactcg attcatttgt ttgctctcct tttcttatcc gtgcatgacc
    34261 aatccgccat cgacattgat gacttgtccg gtgatatatg gattttgtgc taagaaaact
    34321 gctgtttgtg caatatcttc tacttgtccg aaacgctgca tcggaatttg cttttccgct
    34381 tgttccttga ctttgtctgc taaaacttcc gtcatatcag ttgtgatgaa tcctggcgca
    34441 atggcattac atgtaatgcc tctagttgca gcttctcgtg ccacagattt tgttaatcct
    34501 actacgccag ctttactagc tgcataattt gcttgaccaa tatttccaat caacccagat
    34561 acactagaga gattaatgat ggccccttct cgctgcttca tcattttttt caatacgtgc
    34621 tgcgtcatat tgaaggttcc aatcaaattg atatccagac attttttgaa atcttctgca
    34681 tccattcgca tcacaagttt gtcatttgtg attccggcat tgtttactaa cacatggatc
    34741 gatccaagct tttcttctgc ttctttgatc atttgtcccg ctttctcata atcagagatg
    34801 tcaccggaaa ctcctacgca ttttacaccg aatgcttcga tttcttctat cttttctttt
    34861 ggaatctctc cgcgaccatt caaaatgata tttgctccag cttttgcaaa agctaaggcc
    34921 atcgctttgc cgattcctct tgtactacct gtgataaata cattttttcc ttttacatcc
    34981 ataacgttcc tccttatagt attgctattg tttcatcaaa tgtctgctta ttctcaacac
    35041 gcagaactgg tattgtttta tcgatttttt tcataaatcc gcttaatact tttccggggc
    35101 cgacttcgat gacttgttcg atgccgattg tttttaactt atggatactc tcatagaaac
    35161 gaacaggttg catgacttgc tgttcaagta gtcccgcgat tgtttctttc ttcattattt
    35221 ccgttgttgt gttgcttatg ataggaaaag aaggttcaga gaattgaatc tgacttaggt
    35281 ccttcgccag tttttttgct gccggttcta aaatagctgt atgaaaaggc ccgctgacat
    35341 ttagagggat cattcttttg aacccttttt cttttaatag taaaacagct tcgtctactg
    35401 ctttttcttc gccaccaatc acgatctgct gaggtgtatt ataatttgca ggagatacga
    35461 tgccgtactt gctagcttca tgacagcttt cctcgatcgt ttcaatcggc gcattcatca
    35521 ctgcaaccat tttcccgctt ccagctgggg cagcttctgt catataagcg cctctttttg
    35581 ctactaacgc aactgcttca gtgaaggata gcgcattact agctactaat gccgaatatt
    35641 ctcctaaact cagtcctaaa gctgcatctg ggatgattcc atgctccttc aataagcgat
    35701 aatacgcgat gctgactgtt agaatagctg gctgtgtata ttgcgtctga tccaaacgtt
    35761 cattttctgt aaagcaaagt tcagccatat cgtagccgag aatctcactt gcttcatcaa
    35821 atgtctgctt tacaacggct tcttcatata aatcttttcc cataccttga tattgtgcac
    35881 cttggccact aaataagaat gctgttttca tgtttttctc caatatatcc agaatatatg
    35941 tttgattctt cctattcaaa aaggccgaga cattcctatt tcaggaaatg tcccagcccc
    36001 caacctaatt ttaatgatta tgcagtttgt ttttctacgt aagtcactaa atcttgaacc
    36061 gttttgattc cgtcttctgt ttcgattttc acgtcaaatt catcttcaat ttcattgatg
    36121 atttggaata aatccaagct gtcagcttct aagtcttctt ggatgtcagt agtcaatttg
    36181 atttcttcag tttcttttcc taattcttct gcgatgattt cttgaatttt ttcgaatacc
    36241 ataataatat attcctccga tattgtttgc tttttatttt tataatttaa cagtctacaa
    36301 ctccatgaga atacttcccc atgttaaacc tccgccatag cctgtaaata ttactttctg
    36361 ctgttttcct aaggtgatct ttccactaga aactgcttca tccaataaga tagggatgct
    36421 tgcagcagat gtattcccat atttatccat attggtcagg aacttttctt gcggcatctt
    36481 tacttttcgg gcaattttct caataattct gaggtttgcc tgatgcaaaa gcagataatc
    36541 aactggtttg tctttcatga cttcttggat attgtttgca acgtctcgta cggcaaaatc
    36601 aaaaatatct ctgccagtca tttgcaaata atacgaacct tctgaatctt ctgaatagaa
    36661 cggattttta ttttgctggt agccagcggt caatgagcta cttctggtac cgtcagctgc
    36721 taatttttct ttcagcaatt ttttctgtgg attcgcttct aagagaatcc cacctgctcc
    36781 atcgccaaac aacacagcag tcgtacgatc agaccaatcg attagcttag atagtgtttc
    36841 accaccaatc accagtccct tagttgagcc gcagcggatc attttttcag ccatgcttaa
    36901 cgcataaaca aatcctgagc acgcagcact gatatcaaaa gcgatagcat gagttgctcc
    36961 caatttccct tgtacttgtg ctgctacaga tggtgtcgtg taatcgggcg acattgttgc
    37021 tactaaaata aaatccaaat ctttagggtt tagatttttc tgttttatca atttttccgc
    37081 cactaaaatg cataaatcag aagtctcctg atttgttaca catcttcgtt cacgaatacc
    37141 cgtccttgac gaaatccatt catcagaggt atccatcatc ttggaaagat cattattagt
    37201 aataatcttt tcaggaagga cgctggctgt tgcagtaatc attccaaatt gttccattaa
    37261 atatcctcac ttatagtctt gcaagaagtc gtgcaagttt ttcagtgcat ccagcaaggc
    37321 atgctgttcc tccgtagcca ttccgtctaa gatgttcttg accatttcac gatgaaaatg
    37381 ctgatggacc cgaaatagta actttccttt tttcgttagt cccaacttta caacgcggcg
    37441 gtcatcttca ctgcggatac gttcgacata gccttttttt actaacttgt taatagcagt
    37501 ggtcaaggtc ccaacagtaa ttgatagttc tttggctact tctgaagttg tttttttctt
    37561 gtacatcccg attgcctcga tcgtgtgcat ctctgtgatt gacagatctt tgaattgaga
    37621 ttttttcagc tcagactctt cgattgtcaa aatatcattg aagacactaa caagataatc
    37681 atttactgtt tttaaatctg gttccatgtt ccaccgtcca ctaattagtt tgattatcaa
    37741 atcttttgat gttcaaacta tatcttaact tttttcaaaa tgcaacctta aaagatcatt
    37801 tttttactat atgagctttt tttaagattt tactcattta aaacaactga taaatcagca
    37861 tttataattt cttttattag aaaaatcatc tatctcacat attttgatta tcaaagtatt
    37921 ttctgtgaac agaactatag aattccctaa aacttgtcca ttatttcaaa atttgttata
    37981 gtctttttat tcaaagcgta aaaggagatc ctatgttaaa agaaaaaatg aaaactctcc
    38041 ccatgctgcc gggtgtctat cttatgaaag accgagatgg taaggtcatc tatgttggta
    38101 aagcaaaacg gctcaaaaat agagtaagca gctattttca tcagaataaa cagcattcaa
    38161 aaaaagtatt acgtatgatt catcatataa cagattttga tttcgtcgta gtagacacgg
    38221 aattagatgc tttattgctc gagtgtcaat tgatccaaca ttaccgtcct ttttataacc
    38281 gacaaatgaa ttatttcagt aattataatt atgtacatat taccaataag ggatttgtat
    38341 taacagatac tcctacggct cgaacatacg ggccttttcg tctctataaa aaaatgccgt
    38401 ctattttgcg tataatggaa gaaacttatc aaatgccttg gttgtctgaa attagtttgt
    38461 tagctttgcg agtccagctt cctgacctac aggaaatgac ttttgaacag aaaaaaaagg
    38521 aactccaagg gctgtttcaa ggccgaaata aaaagctact gacttacttg aaaaaaagac
    38581 aacaacattt catctatcag ttgaatttcg aaaaagccgg tatgcttcaa aaagatatcg
    38641 aattagttac ttattttatt agacggatcc aggaacaaaa gcagttttta agaacaccat
    38701 cgctcacctt cagcatgcca ttagcagcag atgagagtca gaaaaagcac tacctcgttt
    38761 gttatggtca acttgcagaa acaatgatta cctctggtga cgtttctcct gacttctatt
    38821 atgaaaagaa agaagctcat ttatctttga aacgtcaatt gtcaaaagaa gaaatcgatc
    38881 ctgtacaaat cttgatcagt taccgcaaaa aattagaaaa agaacagttt gaaattgaac
    38941 tgttaaacaa aaaagaagct gaaaagcaac taaattagtt gcttctcggc ttcttttagg
    39001 catcttcaac ttcaaatgtc aattatccta aaatttattg gattaagtca tagatttcca
    39061 tcgctacgat atcgatatta tcaaattcgt attgctgatg agtatctgtt tcagttaatt
    39121 caaatgtatc ggtcgcttta tcataagtta ctgtacactt ctctacgcct tctttttcaa
    39181 aacggcgaac ttgcagttca ttatctgctg actcgaccat agcctccagt cgttttatga
    39241 ttgcgactaa ttgtgaatgt gtcatcggag gtcctccttt tcttatttct gttatattgt
    39301 atcaaaagca tagcctactt gcattgattt tctctaaaaa aactcgtttt ttatgatttt
    39361 ttttctggta tttttccttc aggccaccaa agtttgatca aagcttgggt tccgtcattc
    39421 tcaaaacctt tatctgccaa ctcttcatat agtttcaatg cttgggtagt tgctggcaat
    39481 acaagatcca ttttttctgc ttcttctaat gcaatcttta aatctttgat aaaatgtttg
    39541 acaaaaaaac caggtgtgta atcttctttt aaaatacgag gagaataatt gcttaaagac
    39601 cagttagctg cacttccacc tgaaagcgtc tcaatcactt tctttaaatc aagtccgttt
    39661 ttttgtgcat agacaagcat ttccgtcatt cctgtcatcg tacctgcaat catgatctga
    39721 ttagccattt ttgtatgctg acctttccca gcagaaccat gcaatgtaaa tgtcgtacca
    39781 aattctttga ataaaggcag tacttgatca taaacggctt cttctccacc gaccatgatc
    39841 gtcagtgtac cattctttgc tcctaagtca ccaccggata ccggtgcatc taacgcatct
    39901 gcgccttttt cttttgctgt ttttgcaatt ttttctgcca ggcttggtgt acttgtagtc
    39961 aaatcaacta aaatatgtcc tgagatatct gcagaaaaaa tccccgactg tccatagtag
    40021 atttcttcta catctttggg gtaacctacc attgtaaaga gaatattact ttgttctgct
    40081 accgcttttg gtgtatctgc ccagatagct ccttctgtta ctaaaggatc agcttttgct
    40141 tttgtccgat tatagacagc tacttcgtgt cctgcttcta gtagatggcg tgcgacagca
    40201 cttcccatga cgcctgttcc gataaaacca agtttcattt tttcgacctc ctaaaaatca
    40261 aaaatccttg cttctatgct agtatacgct agaaacaagg attttttcat aaaaaatgtt
    40321 tgaattttat cttttaaatg aatttaattt tattttgatt cgttcaatcg agtcttcttc
    40381 ttcttttctt cgcatctctt cttcatgata aacaaccatg tcttgataaa attcgatcat
    40441 agcttcgccg aaatgtgtag atgaaatatc ataaagttta gcttctaaaa cttttggatc
    40501 tttaggaact tcactggaaa tataatcaat aagtgctggt gcaaagtctt cgtcggtttc
    40561 aaaagttttc cctaatgaag gatgatcgaa caatcggttc aaataagcat ttccttccac
    40621 gacacaaggt gttccggctg ccatcgcttc tgtataagta agcccttgtg tttctgaagt
    40681 agaagcactg acaaaataat ctgccgcttt ataataaagt gctacatcat tgttgtccac
    40741 ttctcctgtg aactgcacaa attcttctaa ctgcagttct gagatcaatt cctctaactc
    40801 ttctacataa ggtccttttc ctacgataac taatctggcg ttaggataag cggagatgac
    40861 ctgaggaaac ccattaatca aggcttggat atttttctca taagaaatcc tgcttaaaga
    40921 aagaatcatc aaatggtttt cctgaagacc tagtgactcc cttaatttgc tgatatcctg
    40981 ctgagtgata tcagaacgtt tgaatttttc aatgtcgata ccagtaggaa tcacgcgaag
    41041 cggtgctatt acaccatagc tgcgtaatgt atcaataaca cgttcacttg gacaaactac
    41101 acctgtcgta tgatttgcaa ataatctaga caagtatttt acatgggatt gcctgatcac
    41161 ttttccttta ccgatataat gaaggtagtc ttcatacatc gtatggtaag tatggatcag
    41221 aggtattttc aacttcttag ctaccatctt cccaagtatc cctgctccga attctgtatg
    41281 ggtatgaata agatctagtt ctagttcttt ggcaaccaaa taagcatacc acatgcctct
    41341 aacaacaact cttcgatcct tgaaagaaat aaatggtaca cttggcattc gaatcacatc
    41401 tttttccaac ttatccgcat taggatcggt cgttgtaaaa atatagactt catgcccatg
    41461 tctttctaat tcttgtttta atgttttgat cgatgtagca actccgctga cttgcggaaa
    41521 gtaggtatcg gtaaaaaagc cgattttcat gtttttcgcc tccaaactag tccttgacct
    41581 gatttacttt ttttaatttg ctagagactt tttcattcag tacactttcg tatacatttt
    41641 taagttcttc accgatttta ttgatacttc gctcttttgc tgtttgataa ccggcttgtg
    41701 tcgtactcgg tagttcttga ttggtaatct gttggatcag tcggataaat tcttcgtttg
    41761 tcttccccat atagcagttc ttgctatctt ctaaccagcc attatacact ggaatatctc
    41821 gaacaagtac gttttgtttg cttgctaaag cttctaaaac aacgatccct tctgtctctt
    41881 cataagaagg gaaaaagaat agatctgcac ctgaatacgc accttctatg atctctccac
    41941 gaatgtaacc aggaaacagg acattttcag gatgatcttc ttttacaatt ttccggatgt
    42001 tccgaggaat cgagtacatg ggaacatgac caaaccaaat aaatgtgtat tcgggcatct
    42061 tcttagcaat ttctacgaaa tcaatgattc ctttacgttc gaaaaacaaa ccgacacaaa
    42121 tgattacttt ttgatccgga gacagtttga aatattctct gaacttttgt tctttcattg
    42181 gatcagggtg atatttctct aagtcgatcc cattggaaat agcttgaatc ggcacacgga
    42241 taccatagct ttccaacaac tgttttgaat aaggtgttgg cgtgatcaga tagtccgctt
    42301 tttggtacaa tcctaccaga tattttttta cgataggaga aagctggttc gatccgataa
    42361 aggaatttcg gaaatcttcc tctgtagaat gcgcatgata gacaactttt tttccttctc
    42421 ttctggcttt attgaccata ttatgactat tgattccgta tgtattgatg tgcaggatgt
    42481 cataatcttc tttttcatct agtgtataag agataccgac ttccgtcaga gcacgttttt
    42541 gatgatcaag cgcacgcccg attccagatt ttgcaagcat cttttcactt tcaaaatata
    42601 ataatacctt caccttacgt cccccttcac ttatatagta taacatagcc tacaaactgt
    42661 cactcaaact taagccctat ttttgtctat tcttactgac aaaaacgttt tcttaaaaaa
    42721 gaacaaaaaa actggcgtag ctcttttaca agccacacca gcttttgata cgttgtttat
    42781 tttacatatt catgaacaag ttcaacgact tcttccatcg tatcgcattc tttcaatgca
    42841 cgatcagcaa gttcagccat tttcgctgtg tccaagcgtt tcattaaact acgcgtttta
    42901 agaatagatg tcgcactcat tgagaactca tctagaccca tacctactag caatggaaca
    42961 gctgtttgat ctccagccat ttctccacac atacctgccc attttccttc agcgtgtgct
    43021 gcatcaatta catttttgat caaacgtaaa attgatgggt tatatggttg gtacaagtaa
    43081 gaaacgcgtt cgttcatacg gtctgctgcc attgtgtatt gaatcaaatc gttcgtacct
    43141 acagagaaga aatctacttc ttttgcaaac ttgtctgcta agactgctgc tgctggaatc
    43201 tcgatcatga tgccgacttg gatcgtatca gatacttcct tgccttcact tatcaatttt
    43261 tgtttttctt cttcaaagat cgcttttgct gcgcggaatt ctttgagtgt agctaccata
    43321 gggaacatga tacgtaaatt accatgaaca gatgcacgaa gcaatgcacg catttgtgta
    43381 cggaacattt cgtcgccttg ttcagataaa ctgatacgca atgcacgata tcctaagaat
    43441 ggattcattt catgaggtaa ttgcagatat ggtagttctt tatctccacc gatgtccatt
    43501 gtacgaacaa ctacaggttt tccttccata ccttctaata cagctttgta agcttcatat
    43561 tggtcatctt ctgttgggaa gtctggagaa tccatataca ggaattcagt acggtacaga
    43621 ccaactgctt ctccaccatt attatggaca ccaactagat cttttggtgt tccgatgttt
    43681 gcagctaatt caaaatgctt gccgtcagct gtgacagttt ctgcattttt taatttttcc
    43741 cattctgctt tttgtgctgc gtaatctgca cctgcttttt caaattcagc tttttgttca
    43801 tctgtaggat cgatgattac atcgccttcg atcccgttga cagccaaaat atcgccttct
    43861 ttgactttag cagtaatttc ttttgtccca acgattgctg gaatttctaa agaacgtgcc
    43921 atgatagcag aatgtgaagt acgtccacca atgtctgtta caaacgcttt tacaaaattg
    43981 cgatctaatt gtgctgtatc acttggagtc aagtcatgtg caacaacgac cacttcttcg
    44041 ttgatcattg atggatttgg taaagtaacg cctaataagt gcgctaaaat acgtttagca
    44101 acgtcacgaa tatctgcagc acgctcttgc atataagcat tatcttccat tgcttcgaac
    44161 ataccgatat acatatccgt tacttctttt agcgctgatt cagcatttac gctgttgtct
    44221 ttaatatttt gcttgatttg acctaccatt tctggatctg aaagaaccat caaatgggca
    44281 tcaaatactt gcgcttctgc ttcacctaag ctttgcgctg ctttttcacg aatttgctgc
    44341 aattcttcag tagatttagc taatgcagca tccaaacgag cttcttcagc cgcagtatct
    44401 tctactgttg ccttactgaa tgttaaatcc ggttgaacta gcaggtaagc ttttgcaacg
    44461 gctacaccgt cactagcggc aatccctttt agcatttcaa ccattattca gataagcctt
    44521 ctttcttcat tgtttcaacg attgctgcca ttgcatcagc ttcgtcagca ccttctgcag
    44581 taatagtaac atcagaacct tgacctacac ctaaagacat aacgcccatg attgatttca
    44641 agtttactga tttaccttta tactctaagt ttacatcaga gttaaatttg cttgctgttt
    44701 gtactaatag tgtagctgga cgagcgtgga tcccagtttc tgctactacg tgaaattctt
    44761 tcttttccat attaatcggt ctcctttaag tgagtgttgt ttttatgtga gggttttctt
    44821 ttttagtaat tcagagaatc ctaacaaatg ccaccctttc atctgtaaag attaacattt
    44881 tttttcgtta gatacaacag ttttcattca atttcagata aaagtattca aattattccg
    44941 gattcttttt catccttttc ttcacatttt ataacaaacg tgtttatttc ctttttcttg
    45001 tcgctttctc ttctcctgac gtttatcatt ctttcctttg accctctttg ttttcatatg
    45061 aaacgaatta cttgcttccc tttttatttt tagtataata agagtcaaag gtcaggaatg
    45121 gtcaatagag atttcacctt ttctacctca taaatctctt aaaaaactag gggttattct
    45181 tttacctcat gaaaaacgag atgtaaaata tggatgtacc catttttgtg aaaggacgtg
    45241 aatgattcat gctttgtcaa aactgcggca agaatgaggc aacgattcac ttgtatgcca
    45301 ctgtcaatgg tcagcggaca caactcgatt actgtcagag ctgttatcaa aaaatcaaaa
    45361 atcaacaaaa tggaggtctg actaatatgg cccaaaatga tccatttgga tttggcagtt
    45421 tggacgatct tttccgttcc atgtctcgcc aaatgcaaca gcaaggaaat tatgagcaaa
    45481 caccaccaac acaatttggc ggaaacaaca acttcaatgg tggtcagcca cctcaaggca
    45541 atacagaagg tttattggga gaatacggaa tcaatatcac agaacaagca cgacaaggtg
    45601 atatcgatcc tgtgatcgga cgcgatgacg aaatcaaacg agtcattgaa attttaaatc
    45661 gccgaaccaa gaacaatcct gtcttgattg gagaaccagg tgttggtaaa acagcagttg
    45721 ttgaaggttt agcacaaaaa atcgtagatg gtgatgtacc gcaaaaatta atggacaaag
    45781 aagtcattcg tttagatgtc gtatctttag tccaaggtac tggtatacga ggacaatttg
    45841 aagaacgtat gcaaaaattg attgaagaga tccgtcaagc agaaaatgtg atcctattca
    45901 tagatgaagt tcacgaaata gtcggtgctg gttctgctgg tgatggaaat atggatgctg
    45961 gtaatatttt aaaaccagct ttagctcgag gagaattgca aatggttggt gctactacct
    46021 taaatgagta ccgcatcatc gaaaaagacg ctgctttaga acgtcgtatg caacctgtac
    46081 gggtagatga accaactgtc gaagaaacga tcagtatttt gaaaggtttg caaaaacgtt
    46141 atgaagacta tcatcatgta aaatatactg atgaggccat cgaagcagcc gcaactttat
    46201 ccaatcgtta cgtccaagat cgcttcttgc cggataaagc catcgatttg ctagatgaaa
    46261 caggttctaa gaaaaacttg acgatccaaa tcgttgatcc taaaacaatc gagaaaaaac
    46321 tgcaaaaagc agaagaacaa aaagttttag cttctcgaga agaagatttc gaaaaagcag
    46381 cttattatcg cgatcagatc aataaacttc aaaaaatgaa agaacgtcag ctcactgaag
    46441 aggaaacacc tgtgattacc gaaaaagata tggaaaaaat cgttgaacaa cggacaggta
    46501 ttccagtggg tgaattaaaa gaaaaagaac agacacaact gaaaaactta gcagatgact
    46561 tgaaagctca tgtcatcgga caagataacg ctgtagatcg agtagcgaaa gcgattcgcc
    46621 gaaaccgtgt tggattaaac aaacagaatc gtccaatcgg ctccttctta tttgttggac
    46681 caactggtgt aggtaaaaca gagcttgcga aacaacttgc ctacgaacta ttcggttcac
    46741 aagattcaat gattcgtttc gatatgtctg aatatatgga aaaacatagt gtctctaaat
    46801 tgattggttc tcctccaggt tatgtcggat acgaagaagc tggtcaatta acagaaaaag
    46861 tacggagaaa tccttacagt cttgtcttgt tagatgaagt agaaaaagct catccagatg
    46921 ttttgcatat gttcttgcaa atcttagatg atggtcgatt gacagatgca caaggaagaa
    46981 cggtcagctt taaagatacg atcatcatta tgacaagtaa tgccggaact ggtaaagtag
    47041 aagcaaacgt tggttttggt gcggcacgcg aaggtgttac tcgttctgta ttgaaccaat
    47101 taaacaacta ctttacacca gaatttctga accgttttga cgggatcatc gaattcagtg
    47161 cattatcaaa agaaaacttg atgacaatcg tcacactaat gctagacgat gtcaaccaaa
    47221 tgctggcagc acagcaactt cacatcgaag ttcctacgaa tgtcaaagaa aaattagttg
    47281 acttaggcta tgatccatct atgggtgctc gtcctcttcg acgcacgatc caagaacaaa
    47341 tcgaagatgg tatcgctgaa ttttatcttg atcacccttc tatccatgag ttgaaagcaa
    47401 aactggataa agatggaaaa atcatcgtta cttctaaacc agaacgtttg gctaaagaat
    47461 cagccgaaga aacggctgaa taaatccagt tttatagcgt gagacggtat ttctttgaga
    47521 aatgccgtct cattcttttt tcagaaaagt caaaatactg ttcattttat aaaaaagaaa
    47581 ttataataag agaaacaagc aattaaaaat acataaaaga ggtgagcgcc atgaaattgg
    47641 taaatgtaac aaacagccat tcccgtcttg tattaaacca gcttgaaaac actgatgccc
    47701 atctagtcaa ggtctacaca gccggaaata ccactattgt ctacactgaa gcccctgaac
    47761 ataatgaaat tctgctgatc aatgacaaac gtaaaatcca gccgaaagaa atcgaagatg
    47821 ctaaaaatta tttcttagat aaaatcaaag acgccgttta ccacgaggat gaaatcaaag
    47881 taatcgaatt ggcaggcctt gttgaaatct cgattccaaa acatgaatta ggcgctacat
    47941 tagccgtgta aacaaatttt gaacataata attaaagagc gtgggacaaa agtaaa
//
LOCUS       DAFEOM010000014        47513 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.014, whole genome shotgun sequence.
ACCESSION   DAFEOM010000014
VERSION     DAFEOM010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.014"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            419..1528
                     /gene="pdhA"
                     /locus_tag="LP524_000938"
     CDS             419..1528
                     /gene="pdhA"
                     /locus_tag="LP524_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HBL2234009.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            1531..2508
                     /locus_tag="LP524_000939"
     CDS             1531..2508
                     /locus_tag="LP524_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBL2234010.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            2533..4176
                     /locus_tag="LP524_000940"
     CDS             2533..4176
                     /locus_tag="LP524_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBL2234011.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            4184..5590
                     /gene="lpdA"
                     /locus_tag="LP524_000941"
     CDS             4184..5590
                     /gene="lpdA"
                     /locus_tag="LP524_000941"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBL2234012.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            5752..6027
                     /locus_tag="LP524_000942"
     CDS             5752..6027
                     /locus_tag="LP524_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HBL2234013.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            6033..6800
                     /locus_tag="LP524_000943"
     CDS             6033..6800
                     /locus_tag="LP524_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HBL2234014.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            6968..8797
                     /gene="typA"
                     /locus_tag="LP524_000944"
     CDS             6968..8797
                     /gene="typA"
                     /locus_tag="LP524_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBL2234015.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            9058..10830
                     /gene="recQ"
                     /locus_tag="LP524_000945"
     CDS             9058..10830
                     /gene="recQ"
                     /locus_tag="LP524_000945"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBL2234016.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGRNKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            10944..11246
                     /locus_tag="LP524_000946"
     CDS             10944..11246
                     /locus_tag="LP524_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HBL2234017.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            11249..12412
                     /locus_tag="LP524_000947"
     CDS             11249..12412
                     /locus_tag="LP524_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBL2234018.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            12438..15866
                     /locus_tag="LP524_000948"
     CDS             12438..15866
                     /locus_tag="LP524_000948"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBL2234019.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            15887..17011
                     /locus_tag="LP524_000949"
     CDS             15887..17011
                     /locus_tag="LP524_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234020.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            17001..17438
                     /locus_tag="LP524_000950"
     CDS             17001..17438
                     /locus_tag="LP524_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBL2234021.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            17422..17760
                     /locus_tag="LP524_000951"
     CDS             17422..17760
                     /locus_tag="LP524_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HBL2234022.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            17879..18439
                     /gene="rsmD"
                     /locus_tag="LP524_000952"
     CDS             17879..18439
                     /gene="rsmD"
                     /locus_tag="LP524_000952"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBL2234023.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            18436..18927
                     /gene="coaD"
                     /locus_tag="LP524_000953"
     CDS             18436..18927
                     /gene="coaD"
                     /locus_tag="LP524_000953"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBL2234024.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            18917..19960
                     /locus_tag="LP524_000954"
     CDS             18917..19960
                     /locus_tag="LP524_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HBL2234025.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            20119..20847
                     /locus_tag="LP524_000955"
     CDS             20119..20847
                     /locus_tag="LP524_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBL2234026.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            20963..21208
                     /locus_tag="LP524_000956"
     CDS             20963..21208
                     /locus_tag="LP524_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBL2234027.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            21205..21423
                     /locus_tag="LP524_000957"
     CDS             21205..21423
                     /locus_tag="LP524_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBL2234028.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            21559..22098
                     /locus_tag="LP524_000958"
     CDS             21559..22098
                     /locus_tag="LP524_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HBL2234029.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            22091..24364
                     /locus_tag="LP524_000959"
     CDS             22091..24364
                     /locus_tag="LP524_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HBL2234030.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            24402..25448
                     /gene="holA"
                     /locus_tag="LP524_000960"
     CDS             24402..25448
                     /gene="holA"
                     /locus_tag="LP524_000960"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBL2234031.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(25537..25791)
                     /locus_tag="LP524_000961"
     CDS             complement(25537..25791)
                     /locus_tag="LP524_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBL2234032.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(26191..26460)
                     /locus_tag="LP524_000962"
     CDS             complement(26191..26460)
                     /locus_tag="LP524_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234033.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(26930..27385)
                     /locus_tag="LP524_000963"
     CDS             complement(26930..27385)
                     /locus_tag="LP524_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBL2234034.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(27523..29451)
                     /locus_tag="LP524_000964"
     CDS             complement(27523..29451)
                     /locus_tag="LP524_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBL2234035.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(29648..30481)
                     /locus_tag="LP524_000965"
     CDS             complement(29648..30481)
                     /locus_tag="LP524_000965"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBL2234036.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(30673..31377)
                     /locus_tag="LP524_000966"
     CDS             complement(30673..31377)
                     /locus_tag="LP524_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBL2234037.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(31400..32026)
                     /locus_tag="LP524_000967"
     CDS             complement(31400..32026)
                     /locus_tag="LP524_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBL2234038.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            32352..34727
                     /locus_tag="LP524_000968"
     CDS             32352..34727
                     /locus_tag="LP524_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBL2234039.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(34751..35242)
                     /locus_tag="LP524_000969"
     CDS             complement(34751..35242)
                     /locus_tag="LP524_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HBL2234040.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            35429..36349
                     /locus_tag="LP524_000970"
     CDS             35429..36349
                     /locus_tag="LP524_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL2234041.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            36503..36847
                     /locus_tag="LP524_000971"
     CDS             36503..36847
                     /locus_tag="LP524_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HBL2234042.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(37037..37537)
                     /locus_tag="LP524_000972"
     CDS             complement(37037..37537)
                     /locus_tag="LP524_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2234043.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            38065..38148
                     /locus_tag="LP524_000973"
     tRNA            38065..38148
                     /locus_tag="LP524_000973"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38099..38101,aa:Leu,seq:cag)
     gene            complement(38472..39110)
                     /locus_tag="LP524_000974"
     CDS             complement(38472..39110)
                     /locus_tag="LP524_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL2234044.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            39445..39879
                     /locus_tag="LP524_000975"
     CDS             39445..39879
                     /locus_tag="LP524_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBL2234045.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            39902..40222
                     /locus_tag="LP524_000976"
     CDS             39902..40222
                     /locus_tag="LP524_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234046.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            40363..40554
                     /locus_tag="LP524_000977"
     CDS             40363..40554
                     /locus_tag="LP524_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234047.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(40625..40882)
                     /locus_tag="LP524_000978"
     CDS             complement(40625..40882)
                     /locus_tag="LP524_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBL2234048.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            41040..41303
                     /locus_tag="LP524_000979"
     CDS             41040..41303
                     /locus_tag="LP524_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234049.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            41327..41515
                     /locus_tag="LP524_000980"
     CDS             41327..41515
                     /locus_tag="LP524_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234050.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(41598..41783)
                     /locus_tag="LP524_000981"
     CDS             complement(41598..41783)
                     /locus_tag="LP524_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234051.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            41998..42369
                     /locus_tag="LP524_000982"
     CDS             41998..42369
                     /locus_tag="LP524_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBL2234052.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(42519..42591)
                     /locus_tag="LP524_000983"
     tRNA            complement(42519..42591)
                     /locus_tag="LP524_000983"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42556..42558),aa:Gln,seq:ctg)
     gene            complement(42637..43065)
                     /locus_tag="LP524_000984"
     CDS             complement(42637..43065)
                     /locus_tag="LP524_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234053.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(43125..43883)
                     /locus_tag="LP524_000985"
     CDS             complement(43125..43883)
                     /locus_tag="LP524_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBL2234054.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            44178..44375
                     /locus_tag="LP524_000986"
     CDS             44178..44375
                     /locus_tag="LP524_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234055.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            44481..44963
                     /locus_tag="LP524_000987"
     CDS             44481..44963
                     /locus_tag="LP524_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234056.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(45021..45617)
                     /locus_tag="LP524_000988"
     CDS             complement(45021..45617)
                     /locus_tag="LP524_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBL2234057.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            46004..47470
                     /locus_tag="LP524_000989"
     CDS             46004..47470
                     /locus_tag="LP524_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBL2234058.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT        15712 a         8182 c         9753 g        13866 t
ORIGIN      
        1 taaatttcaa gctgtttctt ttttttcgga aaaagaatac gatataaaga aactaagaaa
       61 aaaccattat ttgtacgtcc gttcacgatg tgtaaataaa ataaatgttt gcaacaaaac
      121 tgtataacac caacgttttt aaatagaaac agatggttgt aagtttgatt agaaaataga
      181 aaaaatgcag atgaaatata aaacgctttc cttattgtga ttttattcac taatgctgtt
      241 aatatgcgga ttggcataat ttcctttcat cgtgaaaatt tgtgctttta gcttgtattt
      301 ctcataaatc catgataatt tattaatgaa gacggttaaa tagtgataca gttttattca
      361 ctgtttcaat cgcagaatga agagattttc ttatagatga gagaggaatg tgtgacaaat
      421 ggcaaacaaa aaagcaccga tcgacttcca agcactgcta gatgaagtca atgcagattt
      481 cccagtatat caagcacttg atcaagatgg aaacgtagtt aacaaagatt tagtacctga
      541 tttatccgat gatgaactcg ttgaattaat gactcatatg gtatggtctc gtgtattaga
      601 ccaacgttca acagcgctga accgccaagg acgcctaggc ttttttgcac caactgctgg
      661 acaagaggca agtcagctgg ctagtgctta cgcatttgat aaagaagatg ttcttcttcc
      721 aggttatcgg gatgtacctc aactagtaaa acatggttta cctttatctc aagctttttt
      781 atggtctcga ggacatgctg caggtaattc ttatccggaa gaattgaaag ctctaccacc
      841 acaaattatc atcggtgcac aatatgtaca agcagccggt gttgcattag gtttgaagaa
      901 acgaaacaag aaaaatgtcg tcttcactta tactggtgat ggcggttcat cacaaggtga
      961 tttttacgaa gcaatcaatt tcgctggcgc ttaccatgca aatgccgtat tttatatcca
     1021 aaacaacggg tatgcgatct ctacacctcg tgaaaaacaa actgcagcaa aaacacttgc
     1081 acaaaaagct gtcgcagctg ggattcctgg tattcaagtc gatggaatgg atccgcttgc
     1141 agtatatact gtatctaaaa tggcacggga atgggcgatc agtggaaatg gaccagtcct
     1201 aatcgaaaca ctgacttatc gttatggacc acatacacta tctggtgacg atccaacccg
     1261 ctaccgtaca aaagacacag atgacgattg gcagaaaaaa gatccattga tccgcttccg
     1321 taaatatttg acggaaaaag gactttggtc tgaagaaaaa gaagaacaag tcatcgaagc
     1381 aactaaagaa gaaatcaaag cagctattgc tgaagccgac aaagtaccaa aacaaaaggt
     1441 ttcagatttc ttgaaaaaca tgttcgaagt atctccacaa tcaatcaaag aacaaattgc
     1501 aatttatgaa gcgaaggagt cgaaataatc atggcacaaa aaacaatgat ccaagcaatc
     1561 acagatgcct tagcacttga actagaaaat gacgagaatg tcgttgtttt tggtgaagat
     1621 gttgggaaaa acggaggagt tttccgggca actgaaggat tgcaggaaaa gttcggagaa
     1681 gaccgagtat tcgatactcc tttagctgaa tctggaatcg caggattgag tttcggtctt
     1741 gcgttagaag gatttcgtcc agttcctgaa atccaattct ttggttttat tttcgaagca
     1801 atggacgaag tagtagctca aatggctcgt acaagatatc gaatgagcgg cactagaaat
     1861 ctacccatca caattcgttc gccatttggt ggcggtgttc atacaccaga attgcattcg
     1921 gataatttag aaggtttgat cgctcagtct cctggtattc gagtagtgat tccttccaac
     1981 ccttatgatg caaaaggact attgatttct tctattagaa gtaacgatcc ggttgttttc
     2041 ctagaacaca tgaaactata tcgctcattc cgagaagaag tgccggatga agcttacgaa
     2101 gttcctttgg ataaagcagc agttactcgt gagggaacag atgtatctat tatcacatac
     2161 ggagcaatgg tgcgtgaagc aatcaaagca gcagacaatc ttgcaaaaga aaatatatct
     2221 gttgaaatca ttgatcttcg tacagttgcg ccattagatg tagaaacgat cattcaatcc
     2281 gtggaaaaaa ctggtcgcgt agttgttgtc caagaagcac aacgacaagc gggcgttgcc
     2341 gcgcaagtag tttctgagat ctcagaacgc gctatccttt cattagaagc cccaatcgga
     2401 cgagtttctg cacctgatac agtcttccca tttggacaag cagaaaatgt ctggttgcca
     2461 aatgcaaaag atatcgaaga caaagtaaaa gaaatcgcgg aattttaaga aaagaaggga
     2521 cgaaacaaag tcatggctta tcaatttaaa ttgccggata tcggtgaagg aatcgcagaa
     2581 ggtgaaatcg tcaaatggtt cgtcaaacca ggagatacga tcaatgaaga tgatacatta
     2641 ctagaagttc aaaatgacaa atcagtagaa gaaatcccgt cacctgttac aggaacggtt
     2701 aaaaatgtga ttgttccaga aggtaccgta gcaaatgttg gggatgtatt agtagaaatc
     2761 gatgcaccag gacacgaaga caatgaaggg gacagtggtg tagccgcaga atcccaaact
     2821 ccagctaaac cggctgctga acccactgtt gatacagaat cagctggatc gtcaagcgaa
     2881 ggagtcttcc aatttaagtt accggatatc ggtgaaggaa tcgcagaagg tgaaatcgtc
     2941 aaatggttcg tcaaaccagg agatacgatc aatgaagatg atacattact agaagttcaa
     3001 aatgacaaat cagtagaaga aattccgtca cctgtcacag ggacagttaa aaacgtgatt
     3061 gttccagaag gcactgtagc aaatgttggg gatgtattag tagaaatcga tgctcctgga
     3121 cacaatagtg caccgtcaac tagtgcgcca tcagctgaag caccaaaaga aaaagtggaa
     3181 acatcaggtt cagcaagtgt cgttgaagct gccgatccaa ataaacgtgt attggctatg
     3241 ccatctgttc gtcaatttgc ccgtgaaaaa gatgtagata tctcacaagt aactgcaact
     3301 ggaaaaggtg gcagagtaac aaaagaggac atcgaaaact tcttggcagg tggtccaagc
     3361 agtgcaccag cgaaatcaga agcgccagaa gcggcagcac cgaaagaagc agctcctgca
     3421 gcagaaagca aaccagctgc accagcaaaa ccattcaagt ccaacttagg agacttggaa
     3481 gaacgtgtag caatgacgcc aacacgtaaa gctatcgcta aagcaatggt caacagtaag
     3541 catacagctc cacacgtgac attgcatgat gaagtagaag tgtctaaact atgggataat
     3601 cggaaacgat ttaaagaagt tgctgctgca aatggtacga aacttacttt cttgccatat
     3661 gtcgtgaaag ctttaacagc gactgttaag aaatacccag tattgaatgc atctatcgac
     3721 gatgcaaacc aagaaatcgt ttataaacat tactacaata tcggtattgc aactgatacg
     3781 gatcacggac tatatgtacc aaatgtgaaa gatgcagacc gcaaaggaat gtttgctatt
     3841 gcagacgaaa tcaatgagaa agcaaaatta gctcatgacg gcaaactatc tgcagaagat
     3901 atgcgcaatg gaacaattac gatcagcaat atcggatctg taggtggcgg ttggtttaca
     3961 ccagtcatca actatccaga agtagctatt ttaggtgtag gaacgattgc acaacaacca
     4021 atcgtaaatg ctgaaggcga aatcgttgtc ggtcgtgtga tgaaactgtc attaagtttc
     4081 gatcaccgta tcgtagatgg agcaactgcg caacaagcaa tgaataatat caaacgtctt
     4141 ttagctgatc cagagctatt aatgatggaa ggatgataaa caaatggtag ttggagattt
     4201 tgctgtagaa ttagatacag ttgtaattgg tgcgggacct ggtggctatg tagctgccat
     4261 ccgcgctgct gaaatgggac aaaaagtagc aatcattgaa agagagtata ttggcggtgt
     4321 ttgtttgaat gtcggctgta ttccttcaaa agcactgata gctgcaggac accattatca
     4381 agagtcacta gattctgaaa tgtttggagt aactagtgaa aacgtcaaac tagattttgc
     4441 aaaaacacaa gaatggaaag aaaacaaagt tgtccatacg ttgacttctg gtgttggttt
     4501 cctattaaaa aaacataagg ttgaaacaat cgaaggagaa gcgttcttcg tagatgatca
     4561 cacactacgc gtcattcatc cagattcagc tcaaacttat tcgttcaata acgcaatcat
     4621 cgcaacaggt tcccgtccaa tcgagattcc aggatttaaa ttcggaggac gtgtgttaga
     4681 ttctactggc ggattagcat tgaaagaagt acctaagaaa tttgtgatca tcggtggcgg
     4741 tgtgattggt gccgaattgg gcggagcgta cgcaaactta ggagcagaag taacgattct
     4801 tgaaggctca ccacaaattt taccaactta cgaaaaagat ctagtaaaat tagtagaaga
     4861 tgatttcaag aaaaaaggcg taacagtcgt aacgaatgca atggcgaaag aagcagtcga
     4921 taacggtgac agcgtgacag tgaaatatgc agtggacggg aaagaagaat ccgttactgc
     4981 tgattatgtg atggtcactg taggtcgtcg tccgaataca gatgatatgg gcttagaaca
     5041 agcgggtgtc gaagtaggag aacgaggatt gatcacggtg gataaacaag gacgtacgaa
     5101 cgtacctaac atctatgcca tcggtgatat cgttccaggg gcggcattag cacacaaagc
     5161 cagttatgaa gcaaaaatcg ctgctgaagc aatctctggc aaaaaagtag ctgtagatta
     5221 taaagcaatg ccggcagtag ccttcactga tcctgaattg gcttctgtag gtatgacgat
     5281 caaagaagca aaagatgctg gtatcgaagc aaaagcatat aaattcccat tttcagggaa
     5341 cggtcgggca ttatcgcttg gtaaaacaga aggattcatc cgtctagtta cgacgatcga
     5401 agataacgtg cttattggcg cgcaaatcgg tggagtcggc gcaagtgata tggtctctga
     5461 attagcttta gcaatcgaat caggaatgaa tgctgaagat atcgccttga cgattcatcc
     5521 tcatccatct cttggcgaaa tcgtcatgga tgcttcggaa ttagcattag gtttaccaat
     5581 ccatatttga tttgaataat acaggcgaaa cgcaactgaa aaatcagttg cgttttgttt
     5641 tttagaatta tcaataatat tattatgtaa acttaaaaat atgacaactt cttggtttat
     5701 taccacttcc tatgctacac tgaagaggaa tatgaaatag gagagttgaa gatgcaagaa
     5761 tatcagtatc cacttgattt agattggaca acagacgaaa tggtcattgt aatgaagatg
     5821 tgggaagatc tggaacgtgc gaatgaaaaa gggataaaca atgaagcttt tttgaagact
     5881 tatcagcagt ttaaaactgt tgtcaaatcc attggtgagg aaaaacgatt gggacgggaa
     5941 tttgaaaaag cttccggtta ttctttatat cgtacagtgc agcaagcaaa aaaaaatcca
     6001 aacaaaactt tgagaatgaa cgggtgagat aaatggaaca gatgattgat catatcaagt
     6061 cctggcttga agaagctggt gagaaaatca aatcttctca acaaacatta acaatctcgc
     6121 aaaaatcaaa tcgaacggat ctagtaacaa atgtcgatcg aaacatccag aattttctaa
     6181 tcgaaaaaat ccattccttt gatccagagg cgaaaatatt ggcagaagaa gaagggtgta
     6241 atcaattatc ggatctcgat ggaagagtct ttatcatcga tcctattgat ggcaccttga
     6301 attttgtgat tgaaggggaa aatttttgta ttatgctcgc tgtttatgaa tccggcgttg
     6361 gaaagcttgg tttcatttat gacgtgatga gaaatgaact tttttggggt ggaaaaggaa
     6421 taggcgtgta taaaaatgat gagccattgc ctagaccagc aaataaatcc ttatcggaag
     6481 gcttacttgg tgtgaatagt tatcttttcg gtcataataa atatggaatc cgagaaattg
     6541 cggaaagcag catggggaca cgtatgtacg gatgcgctgg tcttgaacta gtggcaatct
     6601 tgaaaggaaa ccacattgct tatatttcaa accttagccc ttgggattat gcagcgggaa
     6661 atgtcttatt ggaagagttt ggtatggtaa caagcggtct ttttggtact cctttgacat
     6721 ttgaaaaaag ggaatactat ttagcaggga cttcttgtgc atatgcagaa atcgcagacc
     6781 ttttgaaaaa agaaggctga aaaaaaggta gaaatcattc acaaaaatga taaaaacgag
     6841 caaaagatta attttctcga aaaaatgaaa acattttact atatttcatg aaaaaagtct
     6901 gctataatta gcagtcgagt aaaatttgcc ctgtcataaa gaaccaataa aaaaggagtt
     6961 agttacattg aattatagaa acgatatccg taacgtcgca attattgccc acgttgacca
     7021 cggaaaaaca acattagttg atgaactatt aaaacaatca gatacattag acgcacatac
     7081 gcaattgcaa gaacgtgcga tggactcaaa tgcgctagaa aaagaacgtg gtatcacgat
     7141 tttagccaaa aacacagctg tagactataa aggtattcgc gtaaacatca tggatactcc
     7201 aggacacgcg gacttcggtg gcgaagtaga acggatcatg aaaatggttg acggcgttgt
     7261 gttggtcgta gatgcgtatg aaggaacaat gcctcaaaca cgttttgtat tgaaaaaagc
     7321 actggaacaa catatcacac caatcgttgt cgtaaacaag attgacaaac cttctgctcg
     7381 tcctgagcat gtcgtagatg aagttcttga actattcatc gaattaggag cagatgatga
     7441 tcagttagac tttcctgtga tttatgcatc tgcactaaat ggtacgtcaa gcttgtcaga
     7501 tgatccagca gatcaagaac caaccatggc acctattttt gatacaatta ttgaaaaaat
     7561 ccctgctcct gtcgataaca gcgacgaacc attacaattc caagtttcat tacttgacta
     7621 caacgattat gtaggtcgta tcggaatcgg acgtgtattt agaggaacaa tcaaagtagg
     7681 agaccaagta gcattaatta aattagacgg aacagttaag aaattccgtg taacgaaatt
     7741 atttggtttc tttggactaa aacgtctaga gattcaagaa gcaaaagctg gagacttgat
     7801 tgctgtctca ggtatggaag atatctttgt cggtgaaaca gttactcctg tggatcatca
     7861 agatgcgtta cctatccttc acattgacga accaacattg caaatgactt tcttagttaa
     7921 taattcacca tttgccggtc gagaaggtaa attcgtaacc gctcgtaaga ttgaagaacg
     7981 tttgatggca gaacttcaaa cagatgtatc cttacgagtt gaaccaacaa actctccaga
     8041 tgcatggaca gtttctggtc gtggagaatt gcatttgtct atcttgattg aaaacatgcg
     8101 ccgtgaggga tacgaattgc aagtatctcg tccagaagtt attgaaaaag aaatcgacgg
     8161 tgtaaaatgc gaaccgtttg aacgcgtgca aatcgataca ccagaagaat atatgggtag
     8221 tgttatcgag tccttgagct tacgtaaagg tgaaatgcaa gatatggtcc atacaggaaa
     8281 cggacaaatc cgtttgacat tcttaacacc tgcccgcggt ttgattggtt attcaactga
     8341 attcctttca atgacacgtg gttacggaat catgaaccat acttttgacc aatatctgcc
     8401 aatgcttcct ggtcaaattg gcggacgtca ccaaggggcg cttgtttcga tcgatacagg
     8461 aaaagcaacg acttattcta tcatgagtat cgaagaacgc ggtacagtct tcgtagaacc
     8521 aggtacagaa gtatatgaag gaatgattat cggggaaaac agccgtgata atgatttgac
     8581 tgtcaacatc acgaaagcaa aacaaatgac aaacgtacgg tctgctacta aagaccaaac
     8641 atctgtcatc aaaaaaccaa aacaattgac gttggaagaa tctttggaat tcttgaatga
     8701 tgatgaatat tgcgaagtaa caccagaaag cattcgcttg agaaaacaaa ttttagagaa
     8761 aaatgctcgt gaaaaagcat ctaaaaagaa aaaataatct gtaaaaggat gaagagagaa
     8821 accagcttgt tataaaaaat agctggtttc tttttttgtc caactctttt ctgtggagaa
     8881 gtcatctttg tcaatggaca taaattcaga aaatacgccg aaagacgttt aaaagacttt
     8941 taaaaaactt atttctagag agtttttgtt tcataataag ataatgtttc acgaaaggag
     9001 atcaacttgt ccttcgaaga aaatcgaagt aaaatggcaa gagaaaggag cgggaagatg
     9061 aacgacttat taaaacaata ctttggctat gatgagttta gacctggtca aaaagaaatt
     9121 atccagaaag tcattgatca ggaaaatgta ttaggaatca tgcctaccgg aagtgggaaa
     9181 tcaatctgtt accagcttcc agctttacta ttagatgggc ttactgtggt tgtgtcccct
     9241 ttaatctctt tgatgaagga tcaggtggac gcggcgaatc aattaggtat acccgccact
     9301 tttatcaata gctcgttaga tggttacgaa acagctagac gcttccaaga aatcgataga
     9361 cagcagtacc gtttacttta tatcgctcct gaacgtttta tcatgccgga ttttatccaa
     9421 gcaatgaagc gatggaacgt ccgtatgatc gctattgacg aggcacattg tatctctcag
     9481 tgggggcatg atttccgccc aagttattta cagatggcga accagctaga tcaactgcca
     9541 aatcgtcctg taattgttgc tttgacagct acagcgactg tgcaagtagc tgctgacata
     9601 aaaagacttt taaaaattcc agaaaacaac catattcaga ctggttttga acgagaaaat
     9661 ctacgatttc aagtaattaa agatcaaaaa aaagaacaat atctaatcga atatttaaag
     9721 atcaacaaaa atcaatctgg tatcatttat gcagcaacga gaaaagaagt agaccgtctc
     9781 tatcatttgc ttaaaaaatt cgatttttcc gtgggtagat atcatggtgg tctaaatgag
     9841 aatgagcgaa cagaaatgca agaagccttt ttatacgatc gcttgcagct gattgtagcg
     9901 acaaatgcat ttggtatggg tagaaataaa agcaatgtcc gttttgttat ccattatcaa
     9961 attcctggct ctctagaagc atactatcag gaagctggtc gggcgggaag agatggtctt
    10021 tccagtgaag ccatcctgct tttctctcct caagatatcc aagtccaaaa gttttttatc
    10081 cagcagtccc aaagagaaga agggcaaaag caaaaagaat atgaaaaatt aaaagcgatg
    10141 acagaatacg tgtatattga gtcctgtctt cagcaataca tcctaaacta ttttggcgag
    10201 acaagcagtc cgtgcaaccg ttgtggtaac tgtttagatg acagagaaat agtagaagta
    10261 acgacagaag cacagatggt tctttcttgc ttaaaacgta tgggtgagaa ctatggtaaa
    10321 caaatgctga tgaaagtctt agcaggatcg aaagaacaaa agctgcaagc tttaggtttt
    10381 ggacatttat ctacctatgg actgatgaaa aaccaatcgc aaaaagaaac gatgcagtta
    10441 attgaatatt tgatatcgaa tggctatctt ctgaccatca atggtgagta tcctgttttg
    10501 aaagtaacag aaagagggat acaagtattg aaaggacaag aatctgttta tcgaaaggaa
    10561 cccaagaaag tccagcagct atcggatgaa gaaaccgata cactatttga agtgctgcgg
    10621 gaactgcgaa cggatctagc atcagaagca ggcgttcccc cgtatgtcgt attttcagat
    10681 tccacattga aagaaatgag ccgaatacgt ccatcaagca gattggagat gcttcagata
    10741 aaaggagttg gccaaagcaa gcttgataaa tatggcgaag cctttttatc tcggataaaa
    10801 aatgcggact ctaacgttct ggataagtag aaagaacgaa tacgtaaaaa tgaataaaaa
    10861 caactggtta aatctgtttt ttttgctcct tatgaaaatc aaatatgtta taattaagga
    10921 attagaatgg gaggtaagca cagatggatt ctttgtcgaa aaaacttgcg atccaagtat
    10981 taaatgatga agcagaacgg atcaaacgat taatccgaaa tcaaaacaac agtctctgta
    11041 tctcacaatg caaagccttc gaagaagtgg tagatacaca gatgtacggc ttttcccggc
    11101 aagtcggttt tgctgtccgt ataggagtaa tcggaaagaa cgagggccag ctgatgttaa
    11161 gtgagctaga acgagaattg aatcagttat atagtgaggt ttaccaagaa atttatgata
    11221 aaaaagaagt tggcaaggag gaatagactt gcctaagaaa gtgaaaaaaa ggcatctatt
    11281 agactatagc atcttgattc cctatttaat tttatgtgta ttgggattga tcatggtata
    11341 tagttctacg tcgtatctat tattagaaaa cggacaaaat ccgtccgcat cagtcattaa
    11401 ccagagtatt ttttgggtgc ttagcttgat tgctatcgca ctactttata aaatgaaaac
    11461 agatgttttg aaaaaccagc gtttgatcat ggcagccatc gcagttttga cgattttgct
    11521 gctgattgtt gtgttttttg gtaaggagat caatggggcg aaaggttggc tgcaaattgc
    11581 aggattctcg atccaacctg ccgaatacct aaaaatcatt tctatctggt acctgtctct
    11641 gactttatct aaaagacaaa acagtgtaca aaaagatttt cttgggactg tgaaacgtcc
    11701 acttgcaatg gttatcggac ttactgcatt agtcgctatt cttcccgatt ttggtaatgc
    11761 ggcagttatc tttttgatta ttttggttct gcttttagcc agcggagtca attatgtgta
    11821 tactttgatt gtaggagtag gaggcttttg tctaagtact tttacgatct ggctgatcaa
    11881 tatcacaaac ggaaagatct tacccggacg tttacagtat atctataatc gatttgctat
    11941 ttaccaaaat cctttctcag acgaattaaa taaagggcat caactagtca acggctacta
    12001 tgccatgttc aacggaggac tatttggcag gggattaggt aacagtatcc agaaaaaagg
    12061 atttttacaa gaagcacaaa ccgattttat ttatgcaatc gtagtggaag aactgggagt
    12121 gattatgggg attttgatat tagcgttgct attctttatg attgtgcgca ttgtccttgt
    12181 aggtatccgt tcaaaagatc cgttcaattc gcttttgtgc attgggatcg gcgcaatgtt
    12241 tttgatccaa gtatttgtca atcttggagg aattactggc gttattcctt tgacagggat
    12301 cacgttccca tttttaagtc aaggaggctc cagtttgctg atgctttcga tttgtgtcgg
    12361 ctttgtcctc aacatcagtg cggatgaaaa acgcaagagc ttaggtttat aataaaaaaa
    12421 gatgtaggag tggttcgatg aaaaaagttt tagttgctaa caggggtgaa atcgctgtac
    12481 gtgtatttcg tgcttgtaca gaattaggaa ttaaaactgt tggaatctat gcagaagaag
    12541 atgaatattc cgtacaccgt ttcaaagcgg atgaagcata tttagtagga caaggaaaaa
    12601 aaccgattga tgcgtatcta gatattgaag ggatcatttc tatcgcgaaa gaatgcggag
    12661 ctgacgctat tcatccgggt tacggtcttt tgtcggaaaa cttgaatttt gcgcaacggt
    12721 gcgaagaaga aggcatcatt tttgttggac ccaaactgca tcatttagat atttttggag
    12781 ataaaatcaa agcaaaagct gcagctatcg aagcggggat tgcttcgatt ccaggcacag
    12841 atggaccaat cgcttctata gacgatgcct tggagtttgc gaaacaatac ggttatccga
    12901 ttatgatcaa ggcagctcta ggcggcggcg gacgcggcat gcgtgtagct cacgatgaaa
    12961 agagtgcaag agagggctac gaacgtgcga aaagcgaagc aaaagcagcc tttggtagtg
    13021 atgaagtgta tgttgaaaaa tacatcgcta atcctaagca tatcgaagta cagattctcg
    13081 gggatacgca tggaaacgtc atccatcttt ttgaaagaga ttgttctgtt cagcgccgtc
    13141 accaaaaagt agtagaagta gcgccttgtg tctctatgaa tgaacagcaa cgtcaaaaaa
    13201 tctgccaggc agctgtccaa ttgatgaaat atgtcgggta tgtcaacgca ggtacagtag
    13261 aatttcttgt tgaaggagat gatttttact ttatcgaagt caaccctcgc gtccaagtgg
    13321 aacatacgat cacagaaatg attacagata ttgatatcgt aacaacgcaa ctgttgattg
    13381 ctcaaggttt ggatctgcat aaagaaatcg gattgcctca gcaagaagga attaaattaa
    13441 atggttcagc cattcaatgc cgaatcacta cagaagaccc gttgaataac tttttaccag
    13501 acacagggaa aatcgatacg tatcgttcac ctggtggttt tggcgtacgt ctggatgtcg
    13561 gaaatgctta cgcgggctat gtagtgacac cgtattttga ttcattgcta gtaaaagtat
    13621 gtacgcatgg tgctaccttt gaaacagcca ttcagaaaat ggaacgttgc ttgaaagaat
    13681 ttagaatccg tggagtgaaa acaaacattc catttatgct caatgtcatc acacatcctg
    13741 aattccaatc aggaaatgca aaaacaacct tcattgatag tacagcaaca ttatttgaat
    13801 ttcctcgact acgagaccgc ggaaacaaaa cgatgaaata tatcggtgaa attactgtga
    13861 atggttttcc aggtatcgaa agtggcgaaa aaccatttta tgaagaacca cgtatgccta
    13921 aagatctaat cacaagaact gattacgtaa cggctaagaa tgtattagat gcgaagggtg
    13981 ccgatgcttt ggtcgaatgg atcaaaggac aggaaaattt gttgttgacg gatacgacat
    14041 ttagagacgc ccatcaaagt ctgctagcaa ctcgtgttcg gacaaaggat ttcaagcaga
    14101 ttgcacgttt gactggagaa ggtcttcctg aactcttttc aagtgagatg tgggggggag
    14161 caacatttga tgtggcttac cgctttttga atgaagatcc ttggcaacgg ttgagaaaaa
    14221 ttcgttcttt aatgcctaat acgttgctgc aaatgctttt cagaggttca aatgccgttg
    14281 ggtattctaa ttatcctgac aatgtattgg tggaatttgt caaagaagca gcagcgcaag
    14341 gaatcgatgt atttcgaatc tttgatagtt tgaactggac tccacaaatg gaaaaaagta
    14401 tccaagctgt acgggatact ggtaaaatcg cagaggcagc tatttgctat actggcgata
    14461 tcaacgatcc aagtcgggca aaatacaatg ttcagtatta taaagacatg gcaaaggaac
    14521 tagaacaact gggtgcgcat atcattgcaa tcaaagatat ggctgggctg cttaaaccgc
    14581 aggcagctta tcgtttgatt agtgaattga aagaaacgac agatctgcca atccatctgc
    14641 atactcatga taccagcggg aatggaatca tcacttattc tgctgcttca aaagcaggag
    14701 tagacatcgt tgacgtggca atgagtgcaa tgagtgggaa tactagtcag cctagcatga
    14761 gcagcttgta ttatgcactg gtcaatggtc cacggctgcc agaaatcacg attgaaaatg
    14821 cacagaaatt aaaccattat tgggaagatg tgcgcatgta ttacaaaccg tttgaaaacg
    14881 gcttgaatgc tcctgaaaca gaagtatata tgcatgaaat gccaggagga cagtattcga
    14941 atctacagca acaggcaaaa gctgtcggat taggtcatcg ctgggatgaa atcaaacaaa
    15001 tgtatcatac tgtcaacttg atgtttggcg atatcgttaa agtaactcca tcatctaaag
    15061 tagttggaga tatggcacta tttatggtcc aaaatgatct tacagaagaa gatatttatg
    15121 aaaaaggcga aacactaagt ttccctgaat ctgttgtgac tttcttccaa ggagaactag
    15181 ggcaacccgt tggcggattt cctaaaaaat tgcaaaagat tattcttaaa ggcagacctg
    15241 cattgaatga acgtccaggt cttttggctg aatcagtcga cttcaacgaa gtaaaaaaag
    15301 aattggcaga aaaaattggc tatgaaccaa aacaagacga agtattaagc tatttgatgt
    15361 atccgcaagt ctttttggac tatcaaaaag catataatca atttggcgat gttacattac
    15421 tggacacacc aacatttttc caaggtatac gtttgggtga aacaatcaat gtacaaatcg
    15481 aacgcggaaa aacgcttatc attcgtttag atgaaatcgg agaacccgat atcgaaggga
    15541 atcgcgtact gttcttcaat ttgaatggac aaagacgtga aatcactatc aaagataatt
    15601 cgatcatcag tacggtccaa acccgattga aagctgaacc gacaaaccga gaacaaattg
    15661 gagcaacgat gtctggttct gttttggatg tattagtgaa aaaaggcgat aacgttaaaa
    15721 aaggtgatac gttgatgatt actgaagcga tgaaaatgga aacggccatc gaagcacgtt
    15781 ttgacggaga agtcgcacat gtatatgtgt cttcaggaga cactatttcg tctggtgacc
    15841 ttttgattga agtgactgag aaataataag cgaaaggtgg ggaaacatga aaagactggg
    15901 tttgttttgg ctgattctgc tattagttat tgtggcattc tacatggaac cagtcttgtt
    15961 tccgccaaaa cttgaagtga aggaaaactc tgatcaacaa atatcaaacc aaatgacaac
    16021 gtggaaatac caggagctta aagcaactgg tttttctact tatatcggaa aaccgatcga
    16081 tcagctggaa aaggattttg gccaagccta tgatcgatta actagcggat ttggttttga
    16141 aacaagatat tatgaaaata aagctagtca tctcacattt gaagcgaaca tccaagacag
    16201 cagagtcaca actgttaaag tactgcaaac agggcctgct gatatcgcgc cgttttattt
    16261 agggatgacg atgcaagatc ttacgaaaat cactactatc tatactaatt tcacttttga
    16321 gtaccatgat acagaagttg gaatcgaact aatggaagag gatatgaatt atcgtccgct
    16381 aattgctttt gataacgaca cttttgctat tttatttttt gatcaaacaa caaacggcct
    16441 attttcagtt gtgtatttag ataaagatag tcttttgaaa ctattgccat atcaagtgtt
    16501 tggtgaaggc cttccacatt atcaagcaga taaatcggct gattgggaat caatcaatcg
    16561 cgttaaagaa cgaaaaagta cgactttatt aaatatgctt agaagagaag acgaacttcc
    16621 catatacaat caaacacttg cctttcaaaa ccacagtcag ctgctactac atgatttttt
    16681 gacaaggcct gaggaaatat tgtcagagga acgtatggct gattggcaaa aatcgctagg
    16741 cagtgctcaa acgacagtga aattctcact gacgaatgat gaattggaga agttaatcag
    16801 taaacaaaaa ttagaaaata ctagtgggct atttattcat ccggcgattg acgctacttt
    16861 ttcgttttta tattggtaca gcgatcctta caaccatgac cgtttcatgg acccgggaac
    16921 ggattctgta ggcattgctt tttctaaaga aaatatgtta gtcttattgc aagaagaaaa
    16981 cggagaaagt agtgatagca gtgatcgtta acgaagagct gtttcaactg gaggatcaat
    17041 gcctccaagt cgctgaaatg ataaaaaaga gtaaaacgat ggcttcttat cttgaacata
    17101 aacagtcaat ggaagaatct gcaaaagttt gccagttgaa agaagcgttc ttgtctaaga
    17161 aagaagcatt tgaaaatgta gcggcatatg gtgagtatgc acctgattat cgtgaaaaaa
    17221 aacgagctgt tcgaaaagca aaaagacagc ttgatatgga tgagcatgtt gcagctttcc
    17281 gatatagtga aacacagctg caaagtctgt tggatcaaat tgtccaagta cttgccgaag
    17341 aagtttctaa tgaaatcaaa gtagatgcag gtagcccgtt ttttgaaacg aaaaaacact
    17401 ttggatgcgg aggtaattgt catgcaatta gacaatagtg aatttgaaat aacaaaacga
    17461 cgcgggctga tcgtatgggt ctacagctta aaacaattaa agaatctgaa aaagttcggc
    17521 ttgttgcatt atgtctcaag aagaatgaaa tatgtcgtgc tttacttaaa tgaagagaca
    17581 ttggaagaaa cagaagaacg aatccgtaaa ttacattttg ttcgaaacgt agaacgttct
    17641 taccgaccgg atatcgaaat gaattttgcc gaaaaaatag gaaacaaaac agtattaaaa
    17701 gacgaaggtt tcgaaattga agagctaaat acagaaatcc gattagctga acatctttaa
    17761 aaatctctta tgagcagttt ttaaataaaa aataacttgg ataacgatcg atttttgatc
    17821 attgtccaag tatttttttg gtatactagt accagtacca tttctaagga gtgagtagat
    17881 gcggataata tcaggaaatt atggcgggcg ccgtttaaaa agtttagcag gggcgaacac
    17941 cagaccaact acagataaag taaaagaatc catattcaat atgattggac catattttga
    18001 tggagagaca gtattggatc tgtttgcagg aagcggtggt ttagctatcg aagcagtatc
    18061 tagaggctgc agtcatgcgg tatgcgtaga caaaaattac caagcgctaa aaatcatcaa
    18121 agaaaacatc gaaattacga aagaaccaga gaaatttacc acaataaaac ttgatgcgga
    18181 caaagcaatc gaacggctag cagcggagaa acaacaattc gactacttgt ttcttgatcc
    18241 gccatatgca aaacaaaaaa taatcgatca aatccataaa atggaagaac tgggactttt
    18301 ttctaaaacc gcagttatcg tatgcgaaac ggataaaaca gtcgaattgc cagaaactgt
    18361 ggctggattc catcaaataa gaaaacagat ttatggcata acggccgtaa ctatctatag
    18421 aaaagaggga aatcaatgaa acgtgcatta ttcccaggaa gttttgatcc ttttacaaaa
    18481 ggtcacttag atacggtgga acgtgcggct aaattattcg atgaggtagt aattggtgtt
    18541 tttatcaata cttcaaagaa aagtttgttt cccccagaag aaagaatgac attgatcaca
    18601 aaagctgttt ctcatcttcc taatgtaaaa gtcatgcatc aagaaaatca attgacggta
    18661 gaaacagcaa aagagattgg tgcagatgcg ttgatcagag gcatacgaag tatcaaagac
    18721 tttgaatatg aacgagagat tgctcagatg aatcatcatc tatatgatga attggaaact
    18781 gtcttcttat tagcaaaacc agaatatagt cacgtgagtt ccagtatttt aaaagaagta
    18841 ctgcactttg gcggagatgt gagcagctac ttaccgccag tgatcaacga agctttagct
    18901 ggaaagcgtg aatcaaatga aagctaaaca atggttgaaa acagtactga ttatcatatt
    18961 tggaatgttg atcattggat ttgtagtagt tcccttgccg tattatgtag aatcaccggg
    19021 agccacgatc gatctgaaag aactaattac ggtcaatgat aaggaagata aaggttctgg
    19081 gtctttctct cttacctctg ttggtatcag gcaagctaca ggtttcttag ctttaaaagc
    19141 taaaatgacc ccttttgaag aattgatcag tgaagaagaa ttgactggtg gggcgacaaa
    19201 tgaagaatat aatcaaatgc aacagtatta tatggattct tcccaaaatg cagcaatcga
    19261 acaagcgtta aaacttgcag acaagcctta taaaatggaa ttcaaaggcg tatatgtcat
    19321 gagtgtagag aaaacatcga atttctttgg caagctttca gttggagata cagtaacaaa
    19381 agtagacggg aaaagctttc aaagtacaga agcatttatg gattatgtaa aaagccaaaa
    19441 agttggacaa acaatcgaaa tcagttattt gcataatggt gaagaaaaaa cagcttctgg
    19501 tgatctgatc gagttaccaa cagataaaaa ggcaggaata ggtatcacgc ttactgacca
    19561 taccgagatc gaatcagata cagatgttcg tattaattct ggctctatcg gtgggccatc
    19621 agcgggattg atgttcactt tagaaatcta tgaacaagtc actggtaaaa acttgcgaca
    19681 tggtaagcaa attgcaggaa ctggaacgat caacagtgaa ggagaagttg gccgaatcgg
    19741 aggaatcgat aaaaaagtgg caagtgcgga taaagctggc gtagagattt tctttgcacc
    19801 agatgatacg atttcagacg atgttaagaa agaatatcct gaaataaaaa ccaactacga
    19861 agaagcaaaa gctgccgcta aaaaattgaa ttcctcaatg aaaatcgtac cagtaaaaac
    19921 agtacaagaa gcgcttgact atctagagaa aatgaaatga aactaaataa ttaaagaggg
    19981 gttgtgacac gagttctgtc acaacccctc tttttcgtca aaaatcttca cgataaaaat
    20041 aaaaacaaaa gaaaaaattg acgtaattag atgttgaagg gacgattttg ccagagctca
    20101 ctgtttcttc aattttaaat gaaaggagta atgaatgatt tttactggtc attctttgaa
    20161 aaaagaacaa tgatagaaaa aatcagcaag aaaatactag cttcacccaa attgatcatt
    20221 tggatgtcta tgatagttct tatcttttgt atcgcaagtg gttttttatt atcaaatttg
    20281 ctattttata gagaaacaga agagactgct gcaaaagaaa tactttcttc gtttagttcc
    20341 agtggagaag atccatcagc tgaaggaaac gatgtagaag aggatgctcg tccaaaggtc
    20401 atgtacaccg atatcaaagg aagtgtgaag gagcctggta tctattcctt ttcttcagaa
    20461 gaacgggtct atgatgtact aaagcgtgca ggtggattat tagaagaagc ggatagcgat
    20521 cgaatcaact tttctgcgaa gatagaagat cagcaagtcc tatatatccc tgctgtaggt
    20581 gaagagctgc cagaacatct tgatcagttg gcatcctcgg aaggaaaaca gcctacagca
    20641 gattccgagc ctcccaaaat aaatatcaat acagccactc catcagaact tcagcagatt
    20701 cctgggatcg gatcagtcaa agcgcaagaa atcattcgat ttagagaaga aaacggatcc
    20761 ttccagaaag tggaagacct gcaggagatt tctggtatcg gtgaaaagac agttgaaaaa
    20821 ctaaaaaact tcgttacaat taaatagaat gattcaaaat cgactggcac gagcaataaa
    20881 tggacgctaa tggatactaa gaataatcca attctaagag cttggcagga cgaggaggta
    20941 gtatttttgg ataggaaata gcttgattgt tcgcaaagaa acattaaaaa aaccaatgct
    21001 gaatgtctat ctccaaaata aaatatctgg gattcatatt atgaatacgg ctgtcagcgg
    21061 caataacagt caagctctac gagaacgctt tgccaaagat gtacttagct atacagcaga
    21121 taaagtattt attctcattg ggacgaatga tctagcagag cataaacaac tttctaaaga
    21181 aacgtaccaa aaaatctgca gtggatgata gttcagctga aaaaagacta tagttctgat
    21241 catgctttct ttatcacgcc accagcagtc gatgagacaa aacaaaaata tcggaataac
    21301 aagttgatca aagaatatat ctctgttctc acagaagtag caaaacgcca tgattgtgaa
    21361 gtatcgattt ttatacggaa tttcttgaac aaatggattc gaagaatttt tataatgggc
    21421 taatggatga cggacttcat ttcggcagtg aagggtagga tcttttgtca gacaggatga
    21481 ttcgttatat ttaaagaata ttttgtttaa gcaatgagta ctttttaatc aacttgaaaa
    21541 aacggaggaa tataaaacat gttagaaaaa tcttcaaaag catgcgatac gaataaagaa
    21601 aaagttactg ataagtcaca taatgaacgc attccttggg accagtactt tatggctcag
    21661 gcagtactgt tatctttacg tagtacgtgt acacgtttag aagtaggagc gacactcgtt
    21721 cgagaaaaaa ggatcattgc tggcggctat aatggagcag tcagtggaga tgtccactgc
    21781 atagatgaag ggtgttacgt cgtggatgga cactgcttgc gaacgatcca tgcagagatg
    21841 aatgcactcc tacagtgtgc aaaacttggt atcccgacgg aaggttcaga aatctatgtc
    21901 acacattttc catgtttggc ctgtacaaag gctttgttgc aagctggtgt gaaagggatc
    21961 aattatctcc atgactatcg aaatgatcct tatgcccaag cgttgatcga acaattaggt
    22021 gtatctgtac accaagttgc tttagactca cagcattttt caaaacttca aaatgaatta
    22081 acaggaaaac atgtctagcg ttttagaaag gcatgttcga aatcggttga tttttccggc
    22141 aattacgtcg atgattatct gtttgttgta ttacactgac aattggggtt atcgtttgct
    22201 tcttgccgga atttttttat ggttttcgct aaaagaagga cgttttatcg cgtacttgtg
    22261 tattttgtgt agcataacgg tatccatttc ttataggcta caagagtatt catttttaga
    22321 ggaaaaaaca acatctgcgc aaattactgt atttacagat acgattcgtg caaatggtaa
    22381 ttttttgaca atggaaggaa ggttatcaga gaataaacaa aagctccagc tttcttatca
    22441 agtaaacaca gaagcagaat tgaaaacatg gcttaacttc cgaggaaagg gaatagaatt
    22501 gactgctgaa ggcgagttca tctctccagc aaaaaagcgg aatctttata attttgacca
    22561 agaagcttac ttcaggaacc atagaatttc tggacggttc caaattcaaa agattgaaca
    22621 gatcaccatg caaaatcact ggcgaaactg gcttcagaga aaaagaggat cttttatctc
    22681 ctttgtccaa tctgaatttc cgcaaaaaac aagtgtatat attaattctt tattatgggg
    22741 ctataaagat gcaggattca gagaaccaga agaaattttt agggagagtg gtttgctgca
    22801 tctgttcagt ctatcgggtc tacacatcca attttattta gggtggctgt attatttatt
    22861 tcgacgtatc ggatggccac tacacgtcag tattattccg ttgagtctac taactatctg
    22921 ttatgtttct attgcaggaa gttctatttc tgttttgcgt gctagtttgc tgtttttatt
    22981 gcgattgcta gtcaaattgc tgaataagca ttattccacc atggacttat ttgcagttgt
    23041 cttttggctt cttttactat ttgatcctcg atttcttttt catgcgggtg gacagcttag
    23101 ttttttcatg agttttttgt ttatcctgat ttcttttgat ggagattggt tgaaaaaaac
    23161 ggcctctcac gtcctgttta ctatgatcac gatgccgttg attgtatggc atttttatga
    23221 gtggcctatt ttaggtagtc tttttacatt actgattgca ccggtattca agtttctttt
    23281 tcttcctggt gtgttatttc tcacactctt caatcgctgg ctgccggaaa ctttactttt
    23341 gttgatagaa gagggattaa tcttatttga gcgaataatc gatttttctt cagggtcgtc
    23401 tctcattata ggacgtttgc cccttgtgct atctgggatg tatattttcg gattactgat
    23461 ttgtatggat aggttgaaag aacaacctgt gaaacacttg ttttatgttg gtcttttttc
    23521 ttttattttt ttgactttgc catttttacg cttttcttca acgatcgctt ttatcgatgt
    23581 cggtcaagga gacagtatct ttttgcaaag tccttttcga aaagaaacga ttttgatcga
    23641 tactggaggt cagttacact ttgaaagaga aaagtgggcg caaggtatga tacgtcctcc
    23701 tgctgaaaga aatatcgttc catatctaaa aggtcagggg ataagtgtaa tcgataaact
    23761 catcttgaca cacgacgata cggatcatgt gggagaatta cccacgttat ctcagcattt
    23821 tactatcaaa aaaatttata tcggttgggg cgctggacga aatgagccat tacaaaggca
    23881 tttatctgaa gctcaaaaaa atggtactga gatcattgaa atcaaacatg gggacaggat
    23941 cagcggttat tatgatcttt atgtattgac tccttttgaa aagggagagg gaagaaatga
    24001 agattcgatt ggtctatgga tggaatataa caataggcgc tttttatttt taggagattt
    24061 gaatcaagca atggaaaaac aattgctttt gatttatcct aatctgaaag ccgatgtagt
    24121 caaattaggt catcacggta gccgtacgtc aagtaacaca gattttcttt cccatattga
    24181 agcaaaacat gggattatat cctgcggcgt aaataatcgt tatggtcacc cgcacagcga
    24241 agtattggag acactagaag aaaatcagat aaaaacatgg cgaacggatc aacaaggaat
    24301 gatcacctat cgctggcatc cagtttttca tcctcatggg ctagtggaaa caatgataga
    24361 ctagtaattc tcatctgctc atggtaggat aatttttgaa agtggtgagt aatttgagta
    24421 tacaagcaga gttacaaaaa atacgtaaag aaaaacttgc atcttgttac ttgattttag
    24481 gaacagaaaa gtttctccaa gatcaagttc ggacagaaat tgtcaaaaaa atccaattag
    24541 agggcgaaga tgacttaaat tttttaagtt ttgacatgga aaatgcaagt ttagatgagg
    24601 tagtagctga agcagagacg ttgccgtttt tcggagatca acggttagta ttcgtagaga
    24661 atccttattt tttaacaggt gaaaaagtaa acaatggaat cgaacaaaat actgatttat
    24721 tgacggctta tttgaaagaa ccattagaat cgactgtatt agtattcttt gcgccatatg
    24781 aaaagttgga tgaacggaaa aaagtaacga aacaattgaa aaaaacagca attatcatca
    24841 atgtccaaca gttgaatgaa aaagaagtac gtcaatatct gatgaatacc ttagagaatt
    24901 cctcaatcaa aatggaacga agtgcaattg atttattctt gaggttgacc gacttagatt
    24961 tatccaaaat gatgagagag ttacagaaat tggtccttta tggacaaaat caacagcaaa
    25021 tcacggtaag agaagtagaa gaattagttc ctaaaacgtt agaacataat atttttgaca
    25081 tgacccaata tattttaaaa gggaagacgg aacaagcttt gcgtttatac gaagatttag
    25141 ttatgcaagg ggaagaaaca atcaaaatta atgcgatttt gctttctcaa ttacgtttat
    25201 ttttacagac aaaatttctt gtaaagattg gttatcagca ggcaaatatt gctgaaacat
    25261 tgaaaatcca tccatatcgt gtaaaacttg ccatgcagga agtaaaaaaa ttcgatgaac
    25321 atttgctggt acagttgttt gatcaattag ttgagatgga ttatcaaatc aaaacgggac
    25381 aaatcgaaaa agaattaagt tttcaactat tcgttttgcg cacagggcag ttgttaagaa
    25441 atcgataaga agaaagacga gggcaaagca tgcgggaagg tcgtttgttt ttgtcctttt
    25501 tttgacaaat aaaaaaatcg gccgaagccg attcttttat ttagcgattt ttttgcttaa
    25561 acgagatttg tcgcggttag ctttgttttt gtggattaga ccttttgatt cagccatatc
    25621 aatagcttta acagcttctt tatataacgc atctacgttg tcagcacctg aagctacagc
    25681 atcttcaaat tttttgattg cagtacgcat agcgcttgtt tgagatgaat ttttcacatt
    25741 agcgttttcg cttgtacgaa cacgtttgat tgcagattca atgtttggca ttagtttcac
    25801 ctccgataac ttaagttctt acgcgtgtta ccgcataatt caacattcat tattatacct
    25861 aaaggcagcg tgctttgcaa tatgaacatg acaagttttt ttccttaaca gtcttttatt
    25921 aggatttttt ttcagtaaaa acggatagta caagctgaaa aaaatcaaaa agacgctatt
    25981 gtgggaggca atagcgtcaa accaaactag atttttattt atctatttaa acaaagtttg
    26041 aatgggaatg tttggtacta agttattata taacaaagag aaccgtttgc aaagtttttt
    26101 ttgaaaaggg atacgattcc ctgttgttct ttgtttttct attaagaaac tgcagttatt
    26161 ttataaccag tatcctttga tcagtaaagg ctattctggt gtaccgatta ttttaatgta
    26221 ttcatcgata ttttttatca acattttttt tatgaccaac tggttccgac ggttccattt
    26281 gccacttact tccagcacat atttatttag gggcaaacgt aagatatatt ttgccaaata
    26341 gcgatcttta agcaggccat tgatcacacg gtcctcaagc tgcaagttga tcccagtcag
    26401 catcattggc tgttcttgta aaacgtacgt atcaattaaa agaccttcaa aagtttccac
    26461 aagcgtcttc ctttcttttc aaagtaacct gaaaacaggc actaattctt ccattttgat
    26521 attcgttgaa gtggatgcga tcattctttc ttttgtccac tgtaatctag tataacaaaa
    26581 aagagcagaa gagccaaaaa gaaaaaagag catcgaattc tataggaagt ctcattggca
    26641 cggcctaaat tattacaaaa cattgattaa aaaaagcccc tgcatcgcag gagcatcccc
    26701 tatatcgttt taataggtca atcaaaaagc agtatcaaac acattttgta tcaaatcagg
    26761 aaataataga taagctaagc ccagaatcaa aagtaccgct agaagaaacc agccgattgt
    26821 tcctatgaaa tactttaatt tttcgaacat tgtatcacct cgttgtcagt atatcacgga
    26881 tcagtaaatt gggggtgtta agaatctcct aaaatagaca aaatttgttc tattttccta
    26941 aagcaaatac ttgtaagata cagtaagtga taccagaagc aatgaccggt ccggcggcga
    27001 ttcctcttaa gaagacaaca cctaaaatcg tgccgaaaac aagtgcaaca gtgatttgtg
    27061 gatcagaagc aatcaatcct acacccttgg aagataagac agccactagc ccaccgcaaa
    27121 aaacagctac ccagccaata ggggatttaa aggcttctaa tagatcacgt agaccgattt
    27181 ctcctgttgc gatagggatt agaatagccg ctgtgatgat cgtcacccca atattgattc
    27241 ctttgccttg aagccaaggg aaaaaggaac gggaggctgg tatgagcttc ataatcaaaa
    27301 caaaagctgc cgctatcatc agactttgat ttttagctaa aattgctaca agtaaaataa
    27361 ttcctaaaaa aagccaactt tccatgaaat atattcctca ctttcttcga aacaatcata
    27421 acatgttttt agaaagttac aaaagtagga attcatcctt caagacctat tcataagctg
    27481 tttttttcat aaaataaaca aaatcataaa aaaacacttt gcttatttga cagaagacag
    27541 ccgcgctttg tatagtggac aagacttaca tttttttgaa gaatcagaaa gatcaggtaa
    27601 aggtcttagt aaacgcaagc cattcagaat aaccaatatt gtgcttcctt cgtgtcctac
    27661 aactccaaga ggtagattta aaaattgcag gaagttagaa gcaatcaaca agagaataac
    27721 aagaatcgaa aaaataatat tttgtttgat gatccgccgc atttttaaag atagccgatg
    27781 actgtagaca aatttattta agtcgctttt cattaagacg atgtctgcga tatccatagc
    27841 ggcatctgtc ccttgcccca tagcgacacc taaagaagcg gctgctaaag caggcgcatc
    27901 attgactcca tcaccaacca tcgcatttac ttggtaaagt tcattctctt ttttgactag
    27961 ttgtgttttt tcttccggag tacagcttgc atagtaatgt tcaatcccta cttgttcagc
    28021 aatggccttt gctgtgccag aatggtcgcc ggttatcata cttgtatgga tgttttggga
    28081 tttaaagtag gaaattactt gttgggtatt tgcttttgga atatctagca gacctaaaac
    28141 agcaattaac tgctgatctt tagaaagata aatcacggtt ttgccttgat tttccaaacg
    28201 ttcgatcatt tccgcaatct ctttgctgat tatcatcgtc tcaggatcat atgcgtgctt
    28261 gccaactttc cattgcgcac ctaaataggt agtttgtaat ccaaaaccag taatttcttc
    28321 tattggtaag tattggattt cttcaggtat tgtgcaatcg aaatattgca aaatcgcatg
    28381 agcaagtgga tgcgtcgttt tttgttccat agcaacaaga agttgttgtg ccaaatcctt
    28441 ttcatcaaag aaaaagctgt cagttacaac tggttttcct ttagtgagcg ttcctgtttt
    28501 atcaaaggca atcgctttta attcagaaag cttttctaaa tggataccgc ctttgattaa
    28561 aatcccattt tttgcagaat ttgaaagagc agctagagaa gctggtgtag cagatgcgac
    28621 aagcgcacaa ggagaagcca caactaataa taccattcct cgataaaagc tttcatccca
    28681 actccaacct aggaaaaagt gggggagaag gatcattaga ggaacagcaa gcaaaatcaa
    28741 ttttacataa ttattttcga ttcgttcgat aaatgaagct gtttgagttg gcgtattctg
    28801 cgcttcttct actaattgga taatcttagc aaagacagtt tcggaactcg tttttgttac
    28861 tcgaatcgtg atgggatttc cttgattcag cgttcctgca tacatctcat cgcctaaata
    28921 tttttctaca ggcacagatt caccgcttaa tgcagactca tcgatcgttg cctgtgtgct
    28981 ttctaaaaag ccatctatcg gaacagctgc tcctttagcg acaaatacca agttatccac
    29041 ctttagttga gaaacatcta cttcttgcat ttgtccattt tcttttaata attgggcttt
    29101 ttgtggttga agattcatca aagcagtaat ttctttttga cttttattag ttgtgtattc
    29161 ctctaatgca ccgcttaaac aaaagatgaa tgtcaacatt gctccttcaa agtaattacc
    29221 gatcaaacag gcaccaatag cagctaaagc catcaataaa tccacattca gtgtccgatc
    29281 attccacaat tctttcaagc cttccgatgt ttgtttatat ccaccgatga taatggcact
    29341 gatataaaaa agtggggtga tggtaagaga gaatcggtcg aagataaaac cgaagatcat
    29401 aaataaaagg cagccgattg ttgctgcaaa agcttttttc tgattagtca tcgttattag
    29461 acctccttgt caaaacaagg atagcataat aaaaatgtaa ttgataataa taacagctaa
    29521 atgagaacga taatggttat catttggtga ttaagcttga tgttgttttt attatttata
    29581 atgattatta ttaaagaaaa taaaaaacca gaacatttct caaataagag aagtgttctg
    29641 gttttttcta cattgcaata aaccaataac caatcaaaat gataccaagg ataatacgat
    29701 accagccaaa agcggtaaag tcgtttttct tcaaatagct tagtaagaat ttgatagcta
    29761 agacggaaac cacaaaagat acgaatgttc ctacaagaag aacaacagtt tcttccattt
    29821 tgaatgaatt cccttcagct aaatatttga cgatcttcaa gccgcttgcg ccaaacatga
    29881 caggaatccc taagaaaaat gaaaactctg tagcgacaaa acgcgaagca ccaatcaaga
    29941 tagatcctag gatcgttgcg ccagaacggg atgttccggg aacaagtgcc aagacttgga
    30001 ataacccaac aagaagagct gcttggtacg taaagtcatt cagagaagtc caacgaggtg
    30061 ttttgttttt gttgcgtttt tcgacaataa taaaggcaat accgtaaacg atcaaaacaa
    30121 tggcaactgg taagaacttg tggaaatttg cttctaacca atcatccaaa ggcaaaccaa
    30181 tgatgatggc tggtatgcaa gcaacgatca ctttaaacca aagtgtccac gtatcttttt
    30241 tttctaccgt tgatttactt ggagaaaatg ggtttaactt gtgaaaataa agaacgacga
    30301 cagccaagat cgcccctaat tggataacga cattgaacat cgacataaat tgtgaactgg
    30361 catccagttt gatgaattca tctgctagga tcaaatgacc agtactactg atgggcagcc
    30421 attccgttat tccttcaata ataccgagaa tcactgcttt gataatattt gcaaaaaaca
    30481 taaattcctt ccttcccaat taagatacat cttatagtat accgttattt tttgagaaac
    30541 ccaatctgtt ttttaatatt aagaaaaact tgagaaataa agcagataag tacacgaaga
    30601 gaaaaaagaa gacaaagccc tcgttcagac tttgcctttt atgattctct ggtaaaaatt
    30661 aaaatatccc cattatagaa acgagtcgcg actatgtgtt ttcccgttat cgatataaga
    30721 cgtatcattg atgccgtgga tagaaaatcg accgtttgca taagtgacct ttgttacgct
    30781 gccatttaaa agcccagcac gaacaggctg tttaggatcg atcagattaa gcaaaaaggc
    30841 gatcgtcaaa ccatgagata cgactaaaac atttccacca ccattttttt cgcagtgatg
    30901 ggcgatatct tcaaatcctg tccatacacg gtctcgcaat gtttcataag tttccgccca
    30961 cccagcggta tctaagttgt atacagcatt agcaatttgt tcaaaagaaa cttctgtggt
    31021 aaacatttca tcatatgttt taaaattcaa tacacgaggg atcacacccc acatttccat
    31081 gtcatatcca ccttcaagag acccgaaaca ccattctcta atcctttttt caataaaata
    31141 aggaatttct tttcccttag aatgttctcc tagaataata cgggcagttt cgattgcacg
    31201 accactgtca ctagaataag caaatgaaaa gtcgacattt tttaacccgc gacctaaata
    31261 atgaatgcct tcttgtcctt gctttgtgag gggtgtatca caccagcctt gtactcgttc
    31321 aattgtattg aacatcgttt ttccgtgtcg tacgatataa agtgtagttt ctgtcatttt
    31381 attctcctcc taagtcgttt taacgattga aaaaaggatt tgtccttttt tcctgttcaa
    31441 tcgtcgttgc atcgccatgt cctggataga ctgcaaattc atcaggtaat acaaacagct
    31501 gggtccgaat actatgaagt aattgttcta aattccctgt atacagatcg gttctaccga
    31561 tacttcctct aaacagggcg tcgccagaaa cgacaaaatc atcaaaaata aagctgacgc
    31621 ttcctataga atgccctggg gtagggacga cgcgaaaaga catgtttcct aaagtataag
    31681 ttttcatttc aaattcatat tctgcaggcg agacaataac atcagcaata tcatcatgtc
    31741 taccgagtcc agaaagattc aaaatcggat cacctagcca tttttgctct agtgggctga
    31801 cataaactgg gatttgataa tacgcgcgta aaggttccac tgcaccgata tgatcgtagt
    31861 gagtatgggt aagcaaaatc gctaccggct gttggtttgt ttttttgatt tcttgtttga
    31921 ttcgttccgc atcttctcca gggtcaatga tcaaaagtgc ttcttcgttg tagaccaaat
    31981 aacagttttc ttcaatcatt cctgttttta atctttcgat atgcatgtgc attcctcctt
    32041 gatagccaaa acttctttca tccattagta taacgcattc attgctagga aacaaaaaca
    32101 ggtacttatt tctctttact tatctgaatt atcagactat atgaaaagta tttggatatt
    32161 ctaaagagaa ggaaagtaag ttttcatagc attttaatat gtccttgtta cattagattc
    32221 atccaaaaat ccaaattcta tatcctatat ccctgcctaa tcagcagggt gtttttttat
    32281 gtttggaaag agagctcttt ttgctaaact aggttttttc aggtaatctt agagagaaag
    32341 aggtggtgga aatgatcaaa cagtcaaaaa tatctgtgcg gcacttagta gaatttattt
    32401 tgcgacgtgg aagtatcgac aatcgtaaaa aaagtaacca tactgcatta gaaggagcga
    32461 gaatccaccg caagctccaa aaagatgcgg gagaaaacta tgaaaaagaa gtctttttaa
    32521 aaacaacagt tcaattagat gaatattacc tgatcgttga aggccgtgca gacggtattt
    32581 tcaaaagaga cggtatttat tatatagatg aaatcaaaac ctccgaacca cgatttgaag
    32641 atttagaacc agaacaagtg gaacttttct ttcatcaagc aagggtgtat gcctatatct
    32701 atagtcaaga acatgattta caagaaataa atctgcaatt gacttacttc cagacggcag
    32761 aagaagtgat cacgagaaag gttgaacatc agacaagaga gcagttagaa acttttttta
    32821 aaaagctgac tgaagattat aaagaatggc ttgtttttca agaaaactgg cgcactgtac
    32881 gcaatgcctc tttgatggct ttgaaattcc cccatgatga gtataggaaa ggtcagcgtg
    32941 aactagcggt agctgcttat aaaacgatcc gtacgaaaca gaaattgttt gtagaagcac
    33001 ctaccggaac aggaaaaaca atctctacgt tattccccgc tttgaaagct gtaggggaag
    33061 aagagggaga aaagatcttt tatctaacgg ctaaaacaat aactagacaa gtagctgaag
    33121 atgctatgac agcattaaaa gatacaggag ctgaagtaaa aagtgtcact ttgacggcga
    33181 aagacaagat ctgcttttta gatgagacga cctgtaatcc agatcagtgc ccatatgcca
    33241 atggttatta caatcggatt aatgaaggtt tatgggatct tttgaatcat gagaatcaga
    33301 tcactcgtga agtcatcgaa acttatgcaa gaaagcatac actttgtcct ttcgaactat
    33361 cattagatgt cagtatctgg tgtgacgtaa tcattgggga ttacaattat ttatttgatc
    33421 caactgttta tttaagacgt ttttttgaag atgaaaaaaa cgaggactat ctgtttttga
    33481 ttgacgaagc acataatttg gtgaatcgtt cccgagagat gtattcggca gaactatctt
    33541 atgaaaaaac gaaacgaagt aaggaagcta taccaaagga attcaaaaaa cttcatcgcc
    33601 gtttcaacaa actattaaaa gaatttgatt cgatccgaga aatagcaaaa gaagatcatt
    33661 gggattatca tcatcaaaaa gcaccggctg aatctttagt aaaggcaggt tatcaattaa
    33721 gcgaaaaaat taaagaatgg ctggcagaat ttcctgagca cccatcacaa gaacaattgc
    33781 ttccttatta ttttgatttg cttcattttt tgaaggtcag tgaattttat gatgatcact
    33841 acgaaacaac agtcgaaaaa acgtatcgcg atctgatagt aaaagagttt tgtattgatc
    33901 caagcttgtt tcttgagcag tcgttggata aaggaaaaag cagtattctt ttttctgcta
    33961 gtttttcacc actctcttat tatcaggaaa cactgggggg acaaaaaagt ctggcttaca
    34021 aactgccgag tcctttccca gaaggaaacc agcaagtttt catcgcaaat tacctagaga
    34081 cgacttatcg aaaaagggaa cacagtctgg caaaattagt agagacgatt catgcttttg
    34141 ctgatggaaa ggtcgggaat tatttcgttt tctttccttc ttatcaatat ttagatcttg
    34201 cggtggaagc attcagacag caatatcccg acagcaatgt gctaatccaa gaaacggaca
    34261 tgaatgagga agaacgcgaa cgatttttaa caaaatttca aacaaatccc gctgaaacat
    34321 tgattggatt ttgcgtactt ggcgggattt tttcagaagg aatcgatttg agaggggacc
    34381 gattgatcgg cagtatgatc gtaggtgtag gtttgccgca gatgaatcat gagcaggaac
    34441 tgatcaaatc atattatgat gagaaggaac accttggatt tgcttacgct tatcagcttc
    34501 ctggaatgaa caaagtcttg caagctggtg gtcgagtgat tcgtggcatg gcggatcaag
    34561 gaatcgttgt attggcagat caacgatttt ctagtttttc ttatcgacgt ttattcccac
    34621 aacattggca aacagcaaga gaagtacgaa gtgttgggga attggaagaa ggaatacagc
    34681 gtttttggtt atcaaaagaa gcagtaaatc aaaaaaacga ggaatgacag acgtcagcct
    34741 cgttttttga ttaacgatag ttagtgaatt gcaactcgat tggtaagtca agtttacgta
    34801 ataaagcaat cacagcttgt agatcatcac ggttcttacc tgtcactcta atctgatctt
    34861 cttggatttg tgcttttact ttcagttttt catttttgat agctgtagtg attttcttcg
    34921 cgttttcacg atcgattccg ttgaccaaat cggcagtttg tcgagctttg ccacctagag
    34981 cgtgttctga ttcggaaaaa tggatatttt tgattggcac atttcttttg atcagcttac
    35041 caaataatac atctttgatc tgttcgattt tgaaatcatc atcgctcaat acaactagat
    35101 tgctgttctc taatttgatt tctactgtag aatccttgaa gtcaaagcga ttggacaatt
    35161 cttttgttgt ttgctgaatc gcatttttta cttcttctgt attgacttct gatacaatgt
    35221 cgaagctagc atcttttgcc ataggaaccc tcctgatata catataaata tttaccatac
    35281 atctatctta tcataggatt agggcagatt cttacctgtt tgctaaaatt cttctcattt
    35341 gagttcgaag tattttattt taatagataa actgttattc ttacatttgg aataccaaag
    35401 taggggagag gtgcttgtga gtaaaattat gactgcgtat cataaaaatg aaagattaaa
    35461 gaaaatagtg gttattttga taaccggatt gaccgctgcg gtcgctttaa attatttttt
    35521 gattccagcc aatgtctttt ctgcagggat gaatgggatc gctcaaatca tttcctcgct
    35581 tttatatgaa tggtttcata tcaagataga cacaggaaca tttatttttc ttttgaatat
    35641 ccctgtgttt gtattagggt ttctcaaagt aggaaaacga gcaacgatcc ttagttttgt
    35701 gaatgttgta tgtgtatcgg tgatgacgat gctgcttcca gtcggacaga tcactgataa
    35761 tattttgatg aatgccctag taggcggtgt tttattaggt cttggcgtag gattatcttt
    35821 aaaaatgggc tttacaactg gtgggatgga tatcatctct cttgtacttt cacaaacaac
    35881 tggaaaaaca gtcggaaact acatgctgat tctaaatggt atcattgtag tcatcgcagg
    35941 cttcttcttt aactgggaaa gtgccttgta tacgattatt tccatttatg caatgactca
    36001 agtaattgat gccatccaca cgagtcatca aaaagtaaca gcaatgatcg ttaccgtcaa
    36061 gccagaagag gtaacagaag aactagcaaa acggatggta cgtggaatga cattactgcc
    36121 gtctgtcggt ggctattcaa aaaaacaagg gaacatgatc atgatggtca tcacccgcta
    36181 cgaaatgtat gatcttgatc agatcgttca tgaaatcgac gacgatgcat ttatcaatat
    36241 tttaccaact cattcagtat ttggccggtt tgcaaatgaa aacgaacaaa aaatgtatcg
    36301 aacaactggc gtattgccag aaataaaaaa tcgttcacaa aaaaaatgag gaatcaggtt
    36361 tattttagaa taaatttaat ctattacatc taataagaac atttttataa atttaaacga
    36421 gaatcttact tctaaattct catttacaag cagctaaaaa aagagtatga taaataaaaa
    36481 tcgtgttaag gagtgtcgta cgatgagcga agcaaaccaa gaacggtctg gacaagaaat
    36541 agaactaatc aaagaacgta ttcttgctgc cttggaaatg gtgattgatc ctgaattagg
    36601 aatcgacatc gtcaatttag gcttgattta tgatatagaa ttcaatcctg aaaatggaga
    36661 aacagtcatt aagatgactc taacaactat gggctgtcca ttagcagata ttttaacaga
    36721 atctatccat gacgcattaa aagaagtgcc agaagtaacg aaagcagaag tcaaactagt
    36781 ctggtatcca gcatggacga cagataagat gagccgttat gcacggattg cgttggggat
    36841 acgatagaaa tcttggctga ctagctatga gaagcaaata ggaaaaacaa aaaagccttt
    36901 tatcctttct agaagaaaag agataaaagg tttctttgtt tataaaattt tgtctgctat
    36961 attataagtt aattaaataa atgtgttcta ttgataaagg acattcaaat ccctttcctt
    37021 ttggaatcct tcttttttac atcagtttga ttttcgtata gtattctttc tcatcttcac
    37081 gataaatcgc agccccattt ttttgttgtg ctttaagact gccaagattt tttttattta
    37141 cagacaaaag tgcctcatca atgccgattt tttttgcctc ttgtagtact aatgctaatc
    37201 cttgtgtggc atatcctttt ttacgataat taggggagat gccgtaacca atatggccag
    37261 cgccgtttct taatgtttcg ttcagtttat ggcgcaaatt aaaaattcct acatactcat
    37321 gttcatcact ttctaaaata aagacggttg cgggaacgta actctcagga agatcgattc
    37381 ctaaagacgt tttttcgcat ttttttacat aatctaaaaa ttcagcaaat gtatagccat
    37441 aagcattgtt gataaatcca ctctcatttg gtccaaagct agtttgtagc tgataagctt
    37501 tctttaaatc tgcttcccat agtttgatca gtttcatcgt attcacctct ttaagtaacc
    37561 ttattcattt gttttctact atagcactat tgttgtaaat agccatgcct atttttattt
    37621 attgtgtcat atcgtattaa gaatcatcga acaatacaaa tatgaattac aataaaaaaa
    37681 ttatttgaag ttcgttcatt cgttacttat gatctctaga agaaagaaaa cgtgaattac
    37741 ttcagcgtct agaattccag aaggatagta atcaacagaa gaaataatca atatccaaat
    37801 gaaagaagat agctgaatcg agaaaaagag gaaaaaagaa atgaagaaaa aaataaaata
    37861 gttactaatc ccagagtcgt tcgtttaaca ttgaaatcga tcatatggat aaaacagatt
    37921 ttttcaccaa ccctcaatag atgatggagt tgctcggatg aattatttgt gtagatggca
    37981 taagggaggg cgtacagaaa tgaaaaagaa caaaaatatg ttcttgacaa aaaacacatg
    38041 taatcctaaa ctaaaagatg tgatgcggct atggcggaat tggcagacgc gctagcttca
    38101 ggcgctagtg agggtgacct cgtggaagtt cgagtcttct tagccgcata atttgttttt
    38161 ttataaagag aaggtacacc tggagcgatg agaaagaaaa taacatatta tctaaaagaa
    38221 tataggaaaa attttaaaca acggaaccag attcaaataa atcaaaaaaa atttgtgtga
    38281 aggtgggaca gtttaaaacc taatagattg attcaaatag tagatttata gattctgata
    38341 caagaaatac tggcaaacga tagttaagga ataaagacta aacttaaact caaaaagagg
    38401 tcatgcgaaa gaataaatcg catgacctct tactatattt tgaatgtgtt taaacgttca
    38461 aataatgaat cttagtacca accgtttgct tcccagaatg ctttcgccgc ttcccaagaa
    38521 ccataacgtg aagtaacata ttgttctgct actctttctt ggtttgcagc agagtagtca
    38581 ccatttaagt aagaagaatc taattggtaa cggccgatgt aacgtccgtt cgttgctgtg
    38641 taagaaccgc ttgattcttt ttgtgcgatc cattcttttg cagagcttgt acttgctgga
    38701 gtagcagcag tttctgttac agcaggagca ggttgtgttt cagcaacagg cgctgcttgt
    38761 gtttccgttt gaaccgtttc ttgtacaaca ggttgttcag cttgaacagg ttcagcttgg
    38821 actggtgctt cttgaacggc tgcttgaggt tcagcagctg cttgtgcaga gccttctgtt
    38881 gggattgtta attgttgtcc tgagtagatc aggttaatat cagaaattga atttgattct
    38941 gcaatggcat tgatcaagga gttatcacca acgtattttt gagaaatcgt tgatagagta
    39001 tcacctgatt gaacggtata agcttcgtcc gcatgagcag tcgttcccat gaagaatgca
    39061 gcgccggcag ccaaagttgt tccaaaaagt aaagttttaa gtgatgtcat gaaaaatctc
    39121 ctttattcgt taaaaagttt tatttgttgt gttcaacgag gaatacttta acacgtgaaa
    39181 atatttcata ggtaaaagaa gcatgacaat ttatgacaaa tccacgtttt ttgtaacaga
    39241 atgatgttcg ctcgatgtga aaaatgatga ggaatttcac ataatgcaga aataatggga
    39301 ttagaacagg tgttctgatg agaaaagaaa tgtataaata acttgaaaga aattgctttt
    39361 gttcatcatt ttatttacat attgcgtata ttttttgtat ttttgcttga gatgttacaa
    39421 taaaagaaaa aggaggcggt tccaatgagt aaaagatacc agaacatcat ggtagcagtt
    39481 gacggatcaa gacaatcgat caaagcgttc gctgaagccc ttgacttagc aaaggacaat
    39541 gaagcccact tatttatcgt gtcaatcatc aacaaagttg agctgaccca tagtgcctat
    39601 gccttttcaa aaatctatgc agatgaaaaa caaagaacag aagtagccat gctgaaaaaa
    39661 atcaatgatg ccaaagagta cggcattgct gagatccacg caatagttga aactggagat
    39721 ccaagaaatt tgattgccaa tgtcatccct ggacaagaaa atattgattt gatcgttatg
    39781 ggagcgacag gaaaaggtgc tatacaacaa gcattagttg gatcgaccgc ttcttatgtc
    39841 gtatcacatg ccccttgcag tgtgttggtc gtgaagtaat gagataaggg aggaatagaa
    39901 aatggttcgt cctcaagaag tccgtgcgcc aaaagaaaaa atagaaattt tagcaattat
    39961 tgaagacggc acacaaacaa agaagggata ctcgatcgct ttagtaaagt ggaaaggaaa
    40021 aaaaggtgtt gctctgagat gggatggaga taaccaacaa gataaaggat tccctgtcac
    40081 agctaacggt tatcatccag cgtggtttgt tctgcctgat aaatttatgg aattatacag
    40141 ctatgattat gcaagagccg cagcatcaat caaagcatta gataaattaa cagaagagca
    40201 gaatgaaatg gaggaaaaat aaaaaagacg aaccaatcag cctttagaaa actgattagt
    40261 tcgaggagta aaaatgaaaa aaggttattt gggatgaaac tagaataatg cgtataactt
    40321 aaataaaact taaagcaata aaatggcaag agggtgaggc aaatggaagc aggaaaagta
    40381 ttacaggaag taaaagcact atgtgcagat aaaaaatatg aagaagcgaa aagcctgatc
    40441 gaaaagaaca aagaaaattt ggaagaaaag tacccgatcg cacaagaatt tatagaatta
    40501 aaacaaggaa cgatccttca ccgattcaaa catttttttg gtataacaga atagaaaaaa
    40561 cgattaaaga tcggcagaaa tttgctgcat ctttaatcgt tttcttttta aacatgcgag
    40621 ggattcaatt ctgattctta tataaaaaat agatccaaat aataagaaaa attactgaaa
    40681 gcgtcaattc gatgatgtgt gttattaata cagacgttgt ttcccaatgc ctaaaagaaa
    40741 aaatttgata agtaaaatat acagcaggta aagaggccag taaaactgaa aacttctttt
    40801 ttgtccattt tatgtaatag aaaagaatgc ttatcataat aaaaaaagga atataaaaaa
    40861 caaattgacc gatcgcgatc atactattgc ctccgataaa taaagtccat gatacaaaat
    40921 aacactttgt gcgaaatggc gcaagcagtt ttgtggattt atccggtaaa atctgtatcg
    40981 attttgaaat tagaggagaa acttgctata atcaaagtga aagaaggagt gggattttca
    41041 tggatcatgt agtgttagaa atcgatttgc atttgatcaa taatattcgg gtgatttact
    41101 atgttgtaag taacagtgtc gaacaacgtg tactgacgaa taaaataaat ggtattttag
    41161 caaagaaaga cgttcaccga ttcaataatg gtgaaggaag ttattatagt atcccagtag
    41221 aaaaaattat ttatacgaca gtcaaagtca gagaagatct agataccaaa aaagcatatg
    41281 aacctatttt tacaacttat tgattgacag cagatggagg aaaaaaatga atccatatga
    41341 acaacttatt caagatttga tcgatggaaa aatcgaaaag attgatgtaa aaagagaaga
    41401 attaatggct tttcgagaag cgtggctgaa gttggagaat cgtaaatatg tggtgggaaa
    41461 agcctcactg aacggcaatg tgacttatca ttacgatccg acacgtttat tttagtatga
    41521 agtattcaga aaagaaccaa aggaggacga tgcagcagca ttgtcctcct ttgattttac
    41581 aaaagtgaaa gttttactta tttgaataga tttttaaatt tgtttttgac actgtcgata
    41641 tttacatctt ttaaaagatt ttgggctttc tctttataag gtcctaattg ttctttatgt
    41701 tcttcgataa atcgttttgc gccatcgaaa ttgccgttct taatcatttc cttgactttt
    41761 tgaaataatt cttcttgatt cattttgaac acatcctttg ctatcaacat gattttttac
    41821 atggacaaaa cgagaaaaac tgatttatcc acacgtcaat aatatcaaat aacgatcatt
    41881 tttactaaaa aaacactcga ttctcgcttc taaagtagga gaaaaatctc caacttggga
    41941 cggatggaat ttctttaact gacccatata ctgaatatat aacaaggagg tagaaagatg
    42001 aaagtgatta aatatttgat tggactggcg gcgattagtg gtattttgct atttggactg
    42061 aaatattata ctgaaggaag ttatggagaa atccctggta tacttgatca gctaaatcca
    42121 ttagttgaaa aaggagaggt ctatgtaaag acacaaaaac cagaggaagt aaatgaatat
    42181 ggtacagcaa ggtatgtgca gaaagctgct gatgccaatg ggaaagaacg aacggttgaa
    42241 tttaatggtc tttctgtgtt aaaagaaaat cattatttga aaattagcaa taaaggagca
    42301 cacatagaaa cgtatgagga agttcctaga gaaaaagttc cggagaaagc attagcaatt
    42361 attgggtaaa tcagtttttg tattaagaat acgcccgatt ttattattca taatttaatt
    42421 atgtaaacta aatacaagta gtaaaaataa tacaataatc cccctgcaaa aagattgttc
    42481 ttttacaggg ggttcatttt ttcagcaagc acatcaaatt gggatggcag ggttcgaacc
    42541 tacaattgct tgattcagag tcaagtgcct taccagtttg gctacatccc atttattcaa
    42601 caaaataatc atagcacttt cctaaaaaaa cgcaagttat ttttcttgat cggatagttg
    42661 ttgctttact cgtaaaaggc ggcgtactag tttcttttct aatccagcag gaagggcaat
    42721 cagttctgct actgacaaga cggtgtccaa taactgatat tctttgacag gttcattcat
    42781 aatatgatag atgtttgcta aacgttcaat atattcaaag cgagtatact ctgttcgagc
    42841 aattagaaat ttcaataaat cggtggcttt ctcgtactct tgatctgcgt aataaacaag
    42901 agattgacga tacaatttga tttccagttc attgagggta gcaggtctgc gacgctcgtt
    42961 ccactcatta atagagactt taccttgaac atttcccttg cagtcacgga caaaaataat
    43021 cggaatttca tttggatcca tttttttatg tgtgaatttt tccattatct acgctccctt
    43081 caaaggtttg gtatcttgag tataagggat ttttgtttga attactagct tttctccaaa
    43141 gttagcatct gtccacccat tgcagtgaaa atcgccgaaa aatcgttgat cggtactgct
    43201 cgatttttta caccataggg atcgttgaca taaatatttt ctttgtcata tccagtgact
    43261 acacaagcat gaacaagagg agtgactctt acttctccat tatttgtggt ccaagtcata
    43321 aaatcagttt catctgggac ttcaaaattg atagtcacag aagtccagac aggatttcct
    43381 ttagatagtg cttttttgat atcatcgagt tgagcatcgg tttttggaaa agtgtgataa
    43441 tccgtttgga cgatttgctg agcaagggag gcaactgggg gaacatagac gaccatagcc
    43501 cagtctccgc cactgatatc accaacaaat ccttcattag gatcgccatg agtcccatct
    43561 gcattgaaaa cagggacgta atccaattgc tcagctaact ggtttttagt tgtttcataa
    43621 ccataaaaag ataaaagcat agaaagcgaa gtgacttcac agccattttc taaagaagga
    43681 gaatcaaact ggctctctaa tggaacatct agtaaaactt ctgctttctc ttttgtcttt
    43741 ttcgtttttt gtgttgaact tttttgtgca gtgttcattg tatgttctgg agaatggata
    43801 tgtaggtaat aacttgtacc gcttaataaa agagcagctg tgccccctag aaacaatagt
    43861 tttttgaacg tttttttctt catttgaaat ctcccttttc agctgtttat ctttcctatt
    43921 ataaatgtat ttttattgga aatggaagta ttatatttta atatgtagga aataattagt
    43981 ttttaaggaa tatgttttaa ttttttgaaa aaaattcttc taaatacaac agctgtttta
    44041 attgtgaaaa cgaaaacagt acgctataat gcctgtaagg ttgattttta actgtaaata
    44101 aaaatcaatg agttaaatct ccaaagaaat agcagtggta atgtggagaa aagttccagc
    44161 agtttaggag gattttgatg acggaagaaa tgattaattt aggagaacaa tatgcatgca
    44221 aaccaattgg ttttacaaaa acggtcattg gtgaagttgt atcaaaaatg acaaactgcg
    44281 ccgtggtcaa agttgcccaa tgtgctgcag aggaccaaga attattagat gaaaaagcaa
    44341 gcatggttgt tgcaaaatat gatacatttg aataaagaaa acaattggac gtgcggcgtg
    44401 ctgcaagtcc aatttctttg agaaggtaaa tcagtattct tttcctattt tctggagaag
    44461 aaaaagcgag gcgagttgtc atggtaacat tctttacggt tctattgttt ttattcgttg
    44521 ttgtgatgtt tttatttctt tggctggttc gaaaagaaat tatttatcgg actgtgcgaa
    44581 atcgatgggt ctacttggtg atacccttct tagtgtcact agtcatttgg tatacactca
    44641 tcagtcatcc tacagcagat gaattggcaa aagggatctt gtcagcaatg atttttatta
    44701 gttttttgtt ggacagtaga ggaatcacag aagaaggact tgttttgaat agctttgaca
    44761 aaaaaggtgt tcctttttct gagatcaata aaattgtatt atatcagcca aaaggttcta
    44821 aaatcgtaaa aatgaatttt ttccgaaacg gatggcgagg accgatgctg aaattttctg
    44881 catcattaga agaattagtt ccatttttgt cccaacgctt aaacgatgag gcagagattg
    44941 atattatgat tgatccagaa taaaaacaga ggttgcgaca agatgtttgt cacaacctct
    45001 gttttttatt tcaaataaaa ttatccaaat acgcggaagc gatcggcatc gttcatgtat
    45061 tctttttccc cagccggtgt ttctggtgaa ccgaatacta attgcgaacg tagtttccag
    45121 tttgaaggga tattccattc ttttgcaact gcttcgtcaa taactgggtt gtaatgttgc
    45181 aagtttgcgc ccaatccttt atcaactaat gctacccaag tattagctgt tgcgattccg
    45241 tttgattgtt ctgaccaatc tgggaagttg tctgcatata attcaaattg ttcttgtaga
    45301 ccttttacca cgtcagtatc tttgaagaat aatactgtac cataaccatt tttgaaacca
    45361 gctaatttgt tttgtgtatt tgggaaagct tctgctggag tcaatgggcg aagtgcttct
    45421 tcagtgattt cccaaagttt ttcatgtgca tctccgaaaa ggataactgc gcgtgttgat
    45481 tgagcattga aagcagttgg gctttcttta attgcttctt tgatcaaagt tgttaattct
    45541 tcattagaaa gtgaaacatt acgtcctaga tgatagattg aacggcggtt tttcaaagtg
    45601 tcagtaaaag ttgtcataat aaaatttctc ctttgatttg cgttttgcat ttttttaatt
    45661 gattacgaaa tgaagtatag caccttacaa aaagtaagta aacagatatg ctcacaaaaa
    45721 ataagcgaag ttcaaaaaac aattttaata agaaaaatcg ttatgtaagg attttcttag
    45781 ttatcgcttt cattgttgtg ttttaggaat ttttgcttat ttgaaaaaaa cagctgatat
    45841 ataattatta aatctctatc ttcttttttt tatgatattg ccattaaaaa ttacttgctt
    45901 ttatagaaaa ttagtttata tttcttaaat aagtttttta gttaaatttt tcggtaaaga
    45961 tgtataatat tttctgaaaa tttaaagaat tggggtatag attatggaaa aacagcaaga
    46021 caagggcttc ttcgggcagc cgaagggatt gtcaactttg ttcttcactg agatgtggga
    46081 acgatttagt tattacggta tgcgtgcatt acttatctat tatatgtacg atacagttgc
    46141 caacggggga cttggattac cacgagcaac agcattagct atcatgtcga tctacggatc
    46201 tttgatctac atgtctagta tcgtcggcgg atgggtatct gaccgagtgt taggagcaaa
    46261 acgcactgta ttcttcggtg gagttttcat tatgcttggc cacatcgtct tggctttacc
    46321 atttggtgta agtacattgt ttgtttcgat ggcattgatt attatcggaa ccgccttttt
    46381 gaaaccaaat gtttctggca tggtcggaca tctttattca aagactgact tgcgaagaga
    46441 tgcaggattt tcgatttttt acatgggtgt caacttaggg gcattattag caccactaat
    46501 cgttggtaca atcggtcaag aatataacta tcatttaggg ttctctttag ctgctattgg
    46561 gatgttcttt ggattattac aatacatcat ccaaggacgc aagacattag acggagtggg
    46621 tacaacaccg ccgaatcctt tgacaactga agaacgtaaa ggattcatcc gtaacttgat
    46681 catcgcactt gttgttatcg ctgcagtatt cggcggggca caactaactg gtcatctgac
    46741 aattgacttt tttgtcaata caatcagtgt attggggatt atcttaccgt tgtattactt
    46801 tatcaaaatg ctgacttcaa aagatgtaac tgctgaagaa aaaccaagag tatgggctta
    46861 tatcccatta ttccttgcag caatcatttt ctggtcatta caagaacaag gttcatctat
    46921 cttagcttta tttgctagtg aacgtacaca atctacgcta tttggtgtaa cgatccaagc
    46981 aagctggtat caatcattga atccaatttt catctttatt ctgacaccag tattcgtaac
    47041 gatgtggacg aaacttggtg aacgtcaacc atctacagta gtgaaattct ctcttggact
    47101 tttatttgca ggattatcgt acttgctatt gatgttgccg ggtatgcttt atggtacaag
    47161 tggaaaagta agtccattgt ggttgttggg aagtttcttc atcgtggtta ttgctgaatt
    47221 gtgtctttca ccagttggtt tgtctgtgac aacaaaatta gcaccacgag catttgaatc
    47281 acaaacaatc gctatttggt tcttagctga tgcttcttca caagcaatca atgcacaaat
    47341 tgcacgcttc tacacaccgg gaacagaatc tgcttacttc ggtattatcg gtgcagtagc
    47401 aatcgtcggt ggtatcttgc tatttactgt gaaagaacca atcaaaaaac taatgggctc
    47461 tattcattaa tcaatgaaca taaaaacagg aattgcttat ttgcagcagt tcc
//
LOCUS       DAFEOM010000015        46398 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.015, whole genome shotgun sequence.
ACCESSION   DAFEOM010000015
VERSION     DAFEOM010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.015"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            <1..884
                     /gene="acm"
                     /locus_tag="LP524_000990"
     CDS             <1..884
                     /gene="acm"
                     /locus_tag="LP524_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HBL2234059.1"
                     /translation="KKKVTNITATKIWNGGTTPRPSIYFKLFRASTNNWEPVPDAETK
                     RLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGYQKIENGLVVTNENKE
                     VISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDDWRYRFTNLPKYRD
                     GQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITPWKNFPEKTEREKI
                     RNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILPKTSSKKSSFAVIIGL
                     LIVTICAGIFLQKYKKV"
     gene            complement(1156..1641)
                     /locus_tag="LP524_000991"
     CDS             complement(1156..1641)
                     /locus_tag="LP524_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBL2234060.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    1731..2076
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2121..2762
                     /locus_tag="LP524_000992"
     CDS             2121..2762
                     /locus_tag="LP524_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBL2234061.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            2781..3416
                     /locus_tag="LP524_000993"
     CDS             2781..3416
                     /locus_tag="LP524_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBL2234062.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            3434..4249
                     /locus_tag="LP524_000994"
     CDS             3434..4249
                     /locus_tag="LP524_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL2234063.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            4568..6004
                     /locus_tag="LP524_000995"
     CDS             4568..6004
                     /locus_tag="LP524_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL2234064.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            6193..8640
                     /locus_tag="LP524_000996"
     CDS             6193..8640
                     /locus_tag="LP524_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBL2234065.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            8870..9937
                     /gene="rlmN"
                     /locus_tag="LP524_000997"
     CDS             8870..9937
                     /gene="rlmN"
                     /locus_tag="LP524_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBL2234066.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(10130..11233)
                     /locus_tag="LP524_000998"
     CDS             complement(10130..11233)
                     /locus_tag="LP524_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBL2234067.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            11786..12334
                     /gene="aac(6')"
                     /locus_tag="LP524_000999"
     CDS             11786..12334
                     /gene="aac(6')"
                     /locus_tag="LP524_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBL2234068.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            12880..14541
                     /locus_tag="LP524_001000"
     CDS             12880..14541
                     /locus_tag="LP524_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL2234069.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(14611..14958)
                     /locus_tag="LP524_001001"
     CDS             complement(14611..14958)
                     /locus_tag="LP524_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HBL2234070.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            15042..15983
                     /locus_tag="LP524_001002"
     CDS             15042..15983
                     /locus_tag="LP524_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2234071.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            15984..17027
                     /locus_tag="LP524_001003"
     CDS             15984..17027
                     /locus_tag="LP524_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2234072.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            17033..18103
                     /locus_tag="LP524_001004"
     CDS             17033..18103
                     /locus_tag="LP524_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2234073.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            18093..19046
                     /locus_tag="LP524_001005"
     CDS             18093..19046
                     /locus_tag="LP524_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL2234074.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            19058..20068
                     /locus_tag="LP524_001006"
     CDS             19058..20068
                     /locus_tag="LP524_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL2234075.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(20353..21174)
                     /locus_tag="LP524_001007"
     CDS             complement(20353..21174)
                     /locus_tag="LP524_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBL2234076.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(21271..21549)
                     /locus_tag="LP524_001008"
     CDS             complement(21271..21549)
                     /locus_tag="LP524_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234077.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            21817..23826
                     /gene="metG"
                     /locus_tag="LP524_001009"
     CDS             21817..23826
                     /gene="metG"
                     /locus_tag="LP524_001009"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBL2234078.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(24305..24892)
                     /locus_tag="LP524_001010"
     CDS             complement(24305..24892)
                     /locus_tag="LP524_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234079.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            25067..26389
                     /locus_tag="LP524_001011"
     CDS             25067..26389
                     /locus_tag="LP524_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL2234080.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            26544..27320
                     /locus_tag="LP524_001012"
     CDS             26544..27320
                     /locus_tag="LP524_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBL2234081.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            27313..27882
                     /gene="rnmV"
                     /locus_tag="LP524_001013"
     CDS             27313..27882
                     /gene="rnmV"
                     /locus_tag="LP524_001013"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBL2234082.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            27875..28759
                     /gene="rsmA"
                     /locus_tag="LP524_001014"
     CDS             27875..28759
                     /gene="rsmA"
                     /locus_tag="LP524_001014"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBL2234083.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            29210..29839
                     /locus_tag="LP524_001015"
     CDS             29210..29839
                     /locus_tag="LP524_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234084.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(29963..31072)
                     /gene="ugpC"
                     /locus_tag="LP524_001016"
     CDS             complement(29963..31072)
                     /gene="ugpC"
                     /locus_tag="LP524_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBL2234085.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            31281..31703
                     /gene="mgsA"
                     /locus_tag="LP524_001017"
     CDS             31281..31703
                     /gene="mgsA"
                     /locus_tag="LP524_001017"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBL2234086.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            32026..32562
                     /locus_tag="LP524_001018"
     CDS             32026..32562
                     /locus_tag="LP524_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBL2234087.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(32602..34365)
                     /locus_tag="LP524_001019"
     CDS             complement(32602..34365)
                     /locus_tag="LP524_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2234088.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(34368..36083)
                     /locus_tag="LP524_001020"
     CDS             complement(34368..36083)
                     /locus_tag="LP524_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2234089.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(36862..37086)
                     /locus_tag="LP524_001021"
     CDS             complement(36862..37086)
                     /locus_tag="LP524_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234090.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            37277..40054
                     /locus_tag="LP524_001022"
     CDS             37277..40054
                     /locus_tag="LP524_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL2234091.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            40135..40554
                     /locus_tag="LP524_001023"
     CDS             40135..40554
                     /locus_tag="LP524_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBL2234092.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            40566..41036
                     /locus_tag="LP524_001024"
     CDS             40566..41036
                     /locus_tag="LP524_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2234093.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            41053..41832
                     /locus_tag="LP524_001025"
     CDS             41053..41832
                     /locus_tag="LP524_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL2234094.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            41819..42640
                     /locus_tag="LP524_001026"
     CDS             41819..42640
                     /locus_tag="LP524_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL2234095.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            42657..43706
                     /locus_tag="LP524_001027"
     CDS             42657..43706
                     /locus_tag="LP524_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL2234096.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYIQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            43719..44723
                     /locus_tag="LP524_001028"
     CDS             43719..44723
                     /locus_tag="LP524_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL2234097.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            45356..46375
                     /locus_tag="LP524_001029"
     CDS             45356..46375
                     /locus_tag="LP524_001029"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBL2234098.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        15302 a         8107 c         9587 g        13402 t
ORIGIN      
        1 acaagaaaaa ggttacgaac atcacagcaa ctaaaatttg gaacggtgga accactccaa
       61 gaccgagtat ttattttaaa ttattcagag caagtacaaa caattgggaa ccagttcctg
      121 atgcagaaac aaaacgatta gacaatggaa taacttcagt gacttgggaa gatatccaac
      181 agtatgatga tagtggaaac gaatatacgt ttaaagtgca agaagtagat caaaacggaa
      241 atgattatgt cccttcaggt tatcaaaaaa ttgaaaacgg gctagtcgtt acaaatgaga
      301 ataaagaagt catttcagtc tcaggacaaa aaacatggga agacaatgag aaccaagacg
      361 gcaaacgacc gacgacgatc acagtaaatc tattagccga cggtcaaccg atccaacaca
      421 aagaagtaag tgaaaaagat gattggcgct atcgtttcac aaatctgcca aaatacagag
      481 acgggcaaga aatcatctat actgtaacgg aagacaacgt gcccgaatac agtacgacga
      541 ttgacgggta taacatcaaa aacagttata caccggaaaa aacaaatata tctataccag
      601 gtagccatat aacaccgtgg aaaaattttc cagagaagac agagagagag aaaattagaa
      661 atctattctc tcaaaaattg caaatatttg ctcatacagg agaatccagc gaacaaagaa
      721 gcaattatat tagtaagaga gctgtaccta aacagataac aaataaatca aaaagcattc
      781 ttcctaaaac aagtagtaaa aaatctagtt ttgctgtaat cattggttta ttgatagtaa
      841 ctatctgtgc tgggatattc ttacaaaaat ataaaaaagt ttagttagaa tataatattt
      901 tttgttaaat atgactgaaa tcaaaagaat aaaattccga tttataatcg aaaaaagaag
      961 catttttata tatgcttctt ttttcgtaga atcaataaat atggatttgg ctaactatgg
     1021 aagaatactc gtattagttt tcagcgttca tactagttgt tacattgttt gattggccaa
     1081 aagaatttct gatatatttt gtgctaaata aagaaaaaga cgcttgaatt gggaaataag
     1141 aaaactactc tatcattaca aattttcata aactggcaga tgttctaagt aaacaagtat
     1201 cttttcaaag tcggaaaaat ccataatcaa cgtagtggta ttgatatttg gatgaaagcc
     1261 tacggtatct tgacgatcaa tagcagcatc gatcacgact tggatttttt tctctttgtc
     1321 gtgcatcaat gctaatggag tcactgtacc tgctggaacg cctaggaggt caggaagctg
     1381 ttcttcagaa acaaaagata agcgcttttc ttttaattgt tcagctagtt gttttaaatc
     1441 ggcgtgcttt tcatcaggca agatgacaag atagatttgt tttccttttc ttgatttcaa
     1501 taatagattt tttacttgcg gaccaggtag ggaaaaaggg acatttttga ctgacgtaat
     1561 cgcaggatga tcgacttttt gaaaagagat acccagtttt tttagtagtt cataagcttc
     1621 ttgttcggtt gcacgactca tcgcgcatca ccctcctttt tttattagtg tatcataaaa
     1681 aagagttgac aaatgaaaaa taatcatgta aattacacgt aaagttaaaa caaatgacat
     1741 cgaaaagaag agtagccgtt aggtgttttt agagagtctc tggttggtgg aaagaggcag
     1801 cagttaaccg tgaaacacat cttggagtcg attttctgaa gcgtatagta ggaaaatccg
     1861 gggagcccgt tacagctgcg gtcgttgcta gatgaccaga taaggctggc ttttccgtga
     1921 ggagtcggta aaaaaaggtg gaaccacgtg attaacgtcc tttgatcttc ggatcaaagg
     1981 actttttgtt ttcatctgaa cggcaacgac tgtgtgaggt tttttctgca aggaaaaaat
     2041 aaacagaggt ggaaccacgt ataatacgtc ctcttggcat ttgccaagag gattttttta
     2101 ttttaaagga ggaaaagaag atggagtatt tgattgaagt tatgccgcaa ttattagtag
     2161 gagcactcac aacattgaaa ttatttttat ttaccttgat tggctcatta ccattaggga
     2221 ttgttttagc attaggattg tctttgcatt ttaagccgct acgctacttg cttcaaacat
     2281 atgtatgggt tatgcgggga acaccgctgt tgttgcaact gatttttatt ttttatgggt
     2341 tgccgacgat tgggatcgtt tttgatcgtt tcgaagcagc actcattgcc tttatcttaa
     2401 actatgcagc ttattttgca gagatctttc gtggagggat acaatcgatt cctcaaggtc
     2461 aatatgaagc agcgcaagtt ttgcaattaa gtccctttca aacggtgacg aaaatcgtca
     2521 tcccacaagt gacaaagatt gttttacctt ctgtagggaa tgaagttatc aacttggtca
     2581 aagattcttc cttgatctat atattaggtt taggtgacgt aatgcgtgct gggaaaatcg
     2641 cgatggaacg cgacgcgact ttactgcctt tgctaggcgt ggcaatcatc tatcttacat
     2701 tgactggtat tctaacaatc ataatgaaac aagttgaagg aaaattaaat tattaccgct
     2761 aactgatagg aggaagaaat atgattgaat tgaagaaagt gaacaaagca tttggtgaaa
     2821 aaaaaataat caaagatttg aatctaatca ttccagatgg gaaaacatta gctatcgtag
     2881 gtccttccgg tggcggtaaa accacgcttt tacgtatctt ggctggctta gaaagttctg
     2941 acagcggaga attttattta gacggtgtac cgttcaaccc attcgagctt aaaatgaaag
     3001 aacaagttgt aggagtcgta ttccaagact tccagctatt tccacatctg aatatttttg
     3061 aaaacattac cttagcacca aaaatggtat tgaaacagac aaaagaagaa tacaccaaaa
     3121 aagcagaaga attagcggct tcattaggga ttagtgattt attgaatatc tatccttatc
     3181 agctttctgg cggacaaaaa cagcgggtag cgattgccag agcattggct atgaatccac
     3241 gggtattggc ttatgatgaa ccaacaagtg ctttagatcc tgaattacgc cagcaagtcg
     3301 agcgtttgat cctatcattg aaagaagatg gggtaaccca aattattgtc acccatgatt
     3361 tagtatttgc agaaaatgtc ggtgatcaat tactaaaagt ttccccaatt cattaagaaa
     3421 ggagaagata gagatgaaaa aaaccagttt tctattcgct ttgatcgcag gtcttcttct
     3481 ttttgcaggc tgttcgaaca aaaaaacttc agcagataat tgggcgacta tcaacgaaga
     3541 gaaaaaaatc gttataggtt tagacgatac atttgttcct atgggattta gagataaaga
     3601 cgggaagctc acaggatttg atattgattt agccaaagca gtctttgaac aatatgggat
     3661 tacggtagac tttcagccca ttgactggtc aatgaaggaa tttgaactga acaatggaac
     3721 gatcgatttg atatggaacg gctattccaa aacgccggca agagaaaaga aagtgcaatt
     3781 tacaaaacct tatatggaaa acgatcaagt gctgatcaca cctaaagaat caggaatcac
     3841 aaactttgga caaatgaata ataagagatt aggtgcgcaa aatgggtcat ctggttatga
     3901 tgtatttacg aaacaaccag aagtattaaa agagatagtc aaaaaccaag atgctgtttt
     3961 gtatgatagt tttaatgaag cattgatcga tctgaaaagc agacgggtcg atggtctgct
     4021 gatggataaa gtatacgcag actattattt gaaacaaaga aacgaactag aagacttcaa
     4081 cataacaaaa ggttcttatg agagtgagga ttttgcagtt ggtgcacgca agtcagatag
     4141 tacattagtg aaaaaaatca atgaagccct agatcaacta gagaaaacag gtgaattcca
     4201 aaaaatatcc gataaatggt ttggcgaaga tgttacgccg agaaattaac aaatagggga
     4261 ctgtgacaaa agacgtgtca cagtcccttt ttccgaataa acagtgttct cctgccaact
     4321 ccttgtgact cggagctgaa tggcaggctc acaacctctt ggtaaacggt gttcaaaagc
     4381 cagtttttcg gtattaagcc aaatttatgc aaaaatgtag aaagccattt tcacaaaatt
     4441 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
     4501 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
     4561 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
     4621 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
     4681 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
     4741 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
     4801 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
     4861 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
     4921 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
     4981 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
     5041 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
     5101 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
     5161 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
     5221 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
     5281 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
     5341 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
     5401 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     5461 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     5521 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     5581 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     5641 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     5701 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     5761 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     5821 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     5881 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     5941 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     6001 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     6061 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     6121 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     6181 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     6241 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     6301 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     6361 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     6421 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     6481 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     6541 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     6601 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     6661 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     6721 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     6781 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     6841 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     6901 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     6961 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     7021 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     7081 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     7141 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     7201 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     7261 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     7321 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     7381 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     7441 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     7501 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     7561 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     7621 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     7681 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     7741 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     7801 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     7861 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     7921 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     7981 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     8041 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     8101 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     8161 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     8221 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     8281 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     8341 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     8401 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     8461 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     8521 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     8581 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     8641 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     8701 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     8761 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     8821 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     8881 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     8941 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     9001 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     9061 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     9121 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     9181 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     9241 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     9301 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     9361 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     9421 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     9481 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     9541 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     9601 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     9661 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     9721 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     9781 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     9841 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     9901 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     9961 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
    10021 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
    10081 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
    10141 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
    10201 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
    10261 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
    10321 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
    10381 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
    10441 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
    10501 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
    10561 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
    10621 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
    10681 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
    10741 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
    10801 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
    10861 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
    10921 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
    10981 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
    11041 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
    11101 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
    11161 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
    11221 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
    11281 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
    11341 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
    11401 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
    11461 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
    11521 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
    11581 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
    11641 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
    11701 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
    11761 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
    11821 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
    11881 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
    11941 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
    12001 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
    12061 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
    12121 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
    12181 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
    12241 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
    12301 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
    12361 cagcttcaag aaataagaag gaatttacga aaattacttc ttgaattttt gtgaaattcc
    12421 aacttatttc cgaagaagct acttttggaa caccgtgtac cagaggttgt gaaaaagtcg
    12481 cttagtttcg agtagtaaga aaaattttgt gaaaatggct ttctacattt ttgcataaat
    12541 ttggcttaat accgagaaac tggcttttga acaccgttta ttcagataag ggactgtgac
    12601 aagacttttg tcacagtttc tttttgttta ttctttttta tctttgtatg gcaataatag
    12661 acaatataac gaaaattatt taatggcttt tggaagacca tggaaaacct tgtacaaagc
    12721 tattttttct gaaaaattcc ttgaaacctc acgaaaaaat cattatttta tgaaaaagac
    12781 aaaaaaaagt gttgattttt taataattat tcattaaact cttaaacaac ttcagaaaat
    12841 attaattatt ctgaaaattc caataaaggg gtacgtaaaa tgaaaaagaa atttacattt
    12901 ggtttggttg cggtatgcgg ccttgtactt gctggatgtt atggcggcag ttcagccaca
    12961 agcagtagtt cagcaaaaag cggttcagca gatggaggag gcgtatttaa cttagtcgtg
    13021 cctcaagaaa tgcctacagc tgacttatca gttgcaacag atacaatcag ttttacagca
    13081 ttgaataacg tttatgaagg aatttaccgt cttgatgaag acagcaaacc acagccggca
    13141 ggagctagcg agttggcaga agtcagtgaa gacggattaa cttacaaaat caaattacga
    13201 gaagacgcaa aatggtcaaa tggtgatcct gttacagcag cggattacgt ttatggctgg
    13261 caacgaacag tagacccagc tacagcttct gaatatgctt acctgtatgc gccagttgaa
    13321 aatgcagaag acattacagc tggaaagaaa gataaatcag aactagggat caaagcagtt
    13381 ggtgactatg aattagaaat caaattgacg aaacaaacac catatttcca atatctgctt
    13441 gctttcccat catttttccc acaaaaacaa tccgtagtgg aagaaaatgg ggatgcctat
    13501 gcatctgcaa gtgataaagc tgtttataat ggaccattca cattagaaga ttttgatgga
    13561 ccaggaacag atacagaatg gacttataag aaaaatgatc attactggga caaagataca
    13621 gtaaaacttt ctgaaatcaa agtcagtgta gtaaaagaat cttctactgc attgaactta
    13681 ttcaaagatg gccaagcaga tgacgtgatc ttgtctggtg agttagcaca gcaaaacgca
    13741 aacgatccag cttatacttc tgttaaagaa gcgcggacaa gctatatcga attcaatcaa
    13801 cgtgaaaaga attcaccatt caacaatgtg aacctaagaa aagctatttc ttattctatc
    13861 gatcgtgaag ctttagtaaa acaagtacta ggtgacggat cagttgtctc tacaggattg
    13921 atcccatcag atatggtaaa aagtccaaca acgaacgaag actttgcaaa agaagctgga
    13981 aaattagttt cttatgacaa agataaagca aaagaatatt gggaaaaagc gaaaaaagaa
    14041 ttgggcgttg attctttaga atttgatttg atggcttctg atgatgattc atcgaaaaaa
    14101 gtaatcgaat atgttcaaaa ctcaatccaa gaaaacttgg atggcgtaac agtcaaacca
    14161 acacctgttc cattctctgt acgtcttgac cgctcaactt cgggtgattt tgacgcagta
    14221 ctaggcggat gggctgctga ctatgcagac ccaagcagct ttactgattt gtttgtcaca
    14281 ggtaattcat acaaccgtgg tcgctggagc aatgcagaat atgacgaagc agttaaagca
    14341 gcttcatcaa cagacgctgg cgatgaacaa gcccgttggg atgatttcca aaaagcaatc
    14401 aagatcatct ctgatgatat gggtgtcgtt ccggtgtacc aaaaagcaga aggtcaccta
    14461 gtgaatgaaa aaatcaaagg tatcgttcac catgcggcag gcgcttcatg ggattacaaa
    14521 tggacatacg ttgaagaata atcgttaata ccgtgagcat gagaaaaaac gaggctgcgg
    14581 caaatgtcgc aacctcgttc tcatattcac ttatcctaac aaagaaaaaa gcatgaaaat
    14641 agcagaaaga gcaattagta ttccggcaat gatcagccaa aagctgtaca taccgtagct
    14701 ggcagacgag agcgaggaaa attctggttt tttctttcgg ttccttgttc gtgcaagatg
    14761 gaccgatcgc tgaaaatgat cgaagaaacc cgaagaaaag acccataaaa aaccgccgat
    14821 aatcagaaac ggcaatgacc ataagaatag atcattagat aattgaatga gtgtaaagga
    14881 atggttgatg agatttttta ttaaaataac cagaatcagt agaaaagcta ctgcgtaggg
    14941 gatagatttt tttttcattt gattgctcct ttatttttgg ttcattatct aataatgtag
    15001 cgaaacatct gtcagaagtc aaaagctagg gggaaacatt agtgaatagt tatatcaaat
    15061 atgtattaaa acgtgtcttt ttcatgatca tcacgctttg gttaattgca acaatcacct
    15121 ttttcttgat gcaattgctg ccgggcacgc catatacgaa tcaggaacgt ttgagtcctg
    15181 aaacaatcga gatgctaaat aaacaagtcg gtttggataa accagttatc gtacaatatg
    15241 ggatttatct ttctaatttg cttcaaggag attttggaat ttccttccaa tttaaaaacc
    15301 agccagtcgc acatttgtta gctggaagaa tcggaccatc cttgcagtta gggttacagg
    15361 caattatctt tggaacagtc ctagggacga ttttaggaac gatctctgcc atgaagcaaa
    15421 atacatgggc agatacatct tctacactcg tagcgatatt aggtcgttcc attccaaact
    15481 ttgtatttgc tgttttatta caatatatct ttgcaatcaa attacaagtt ttgccaatcg
    15541 caaaatggga tggctttgtg tacacaatct taccaacaat tgcgttggcc atgtcgccgc
    15601 ttgctgactc tgctcggttt atccgtacgg aaatggtgga agtactgcat agtgattatg
    15661 tggaattagc aaaagcaaaa ggattgagcc gttgggaaat cgcctttaaa catggtcttc
    15721 gaaatagttt gattccattg atgactttat taggaccgtt agctgtagca ttgatgaccg
    15781 gatcactggt ggtagaaaat atctttgcta ttcctggtat cggggaacaa ttcgttaagt
    15841 caatcacaac aaatgattat cctacgatca tggctgtaac gatcctttac tcatttatgc
    15901 tgatttttgt tattttgatc gtggatctat tgtacggtct agttgatcca agaattcgtg
    15961 tttcagaagg gagccgagga taaatggaaa tgataccaaa gcgttatgaa acagttgcag
    16021 atatccctgc ggatgaattt cttccattac aaaaaaatac cgaagaagaa cgtgaacaga
    16081 ttactgcacc ttctttgaat tttatccaag attcatggcg ccgtttaaag aaaaacaaag
    16141 ctgccgttat ttcaatggtt ttactattgg tcatcatttt gatctctgtg atcacaatct
    16201 ttgtttctcc tcatgatcca actgcccaaa acgtggctta tatcaatttg cctcctcgta
    16261 ttccgggaat caatatcgat ggattgaatg gaaaagcaat ggttggtggt gaacttgttg
    16321 ataaatatgc tcaagcaaac gtcccttcaa acgtaaattt ctttttagga acagacggtt
    16381 tgggacgtga tgtattaagc cgcttattta tgggaatgag aatctcgttg ctgatcgcat
    16441 ttattgctgc attactagat gtaacgattg gtgtagctta cgggttggtt tctggtttgt
    16501 taggcggacg tgtcgataac gtgatgcaac gttttttgga aatcttatca ggaattccaa
    16561 acctcgttgt catgattttg atgttggtcg tttttgaacc aggaattttt tcaatcgtgg
    16621 ctgctatggc catcacaaac tggataccaa tggcacgtat cgttcgtgca caaacaatga
    16681 aattaaaaga tcaagaatat gttttagctg gattgacatt aggtgaatca agaatgaaaa
    16741 ttgcattcaa acatattttg ccgaatatct ctagtgtcat tattattcaa atgatgttta
    16801 gtattccatc tgctattttc ttcgaagcct ttttaagctt tatcggttta ggtcttacgc
    16861 ctccgtcagc ttcattagga acgatgttga gcgaagggta caagacattc ctgtatctgc
    16921 cttatctatt atggattccg gcagtaactt tatcggttat catgattggc tttaacttac
    16981 ttgctgatgg attgcgtgat gcctttgatc ctaaaatgaa agagtgaaga cgatgacgaa
    17041 aattttagaa gtgaaagact tagaaatctc ttttgatacg tatgctggaa aggtacgagc
    17101 gattcgcggt gtcaattttc atttgaataa aggagaaaca ttggcgatcg ttggggaatc
    17161 tggaagtgga aaatcagtga cgaccagaag catcatgcgc ttgctgtcaa gcaacgccaa
    17221 tatagatgct ggtcaaatcc tctttaaagg taacgatatc gttggaaaat cagaaagaga
    17281 gatgcaaaaa atccgtggta aagaaatagc gatgattttc caagatccaa tgacctcttt
    17341 agatccgacg atgcccattg ggaagcaagt cgctgagtcc cttagaaagc ataataaagt
    17401 ttcaaaaaaa gagggacaaa aagctgcatt agaattgctt aaattagttg gaatacccga
    17461 agctgagaaa cgtatcaaca gttatccgca tcagttctca ggaggtcagc gtcaacggat
    17521 cgtgatcgcg attgcactgg tatgttatcc ggaaatcctg attgctgatg aacctacaac
    17581 agcgcttgat gtgactattc aagcacagat tttggagctg ctgaaagaaa ttcagacaaa
    17641 aatcgataca tcaattatct ttatcactca tgaccttggt gtagtagcga atgtcgctga
    17701 tcgtgtcgct gtcatgtatg gaggaagaat cgttgaagtt ggaacatcgg aagaaatttt
    17761 ctacaacccg caacatccct atacttgggg actgcttggt tcaatgccga cattagatag
    17821 tgcgaacgat cgattgtatg ctattcctgg ttctccaccg gatttattga acccgccaaa
    17881 aggagacgca ttctatccaa gaaatgaatt cgcgatgaaa atcgatgcag aacaagaacc
    17941 accatttttt gaactatcaa aaacacacaa ggcagcaact tggctacttg ctccgcaggc
    18001 acccaaagtg acaccgccag aagaaatcca acgtcgctgg gctatcttta aagagaaaca
    18061 aaaacatact tacggtcaag gaggaattgc tgatgccaaa taaactactt gaagttaaag
    18121 gattgaaaca gtatttcaat gtaggaaaaa agaatgaagt acatgctgta aatgacatca
    18181 gctttcacat ttatgaagga gaaacattcg gtttagttgg agaatctgga agcggaaaat
    18241 ccactactgg ccgtacgatc attcgcttga atgaacctac tgatggtgag atattattcg
    18301 acggtcaaga tataacgaag atcaaagata aaaaaggttt gacgaaattc cggcatgatg
    18361 ttcagatgat ctttcaagat ccatacgctt cgttgaatcc tcgtatgaaa gtcagagata
    18421 tcattgccga aggaattgat gttaatggac tagctaaatc atctgaagag cgtgcaaaaa
    18481 aagtcgatga tctattgaga actgttggat tgaatccaag ccacggtact cgttatcctc
    18541 atgaattttc aggtgggcaa cgccagcgta ttggtattgc acgagcattg gcagtaaaac
    18601 cgcgttttat catttgtgat gaaccaattt ctgcactaga tgtgtcgatc caagcccaag
    18661 tggtcaattt gttgcaagat ctccaaaaag aacatcaatt gacctatcta tttattgcac
    18721 atgatttgtc gatggttaaa catattagtg accgaatcgg tgtaatgcat aacggcttgt
    18781 tattagagat gggaacaagt gatgaaatct ataatcacgg tgttcatcct tatacagaaa
    18841 gtttgttatc tgctattcct ctaccagatc cagaccatga gagaaaacgc agaagaatca
    18901 aatatcaacc tgaaccagat gacggacaag tacgtcaact tcgtgaaatc gcaccagaac
    18961 attttgtcta tgcaactgag caggaagtcc catattacgc aaaaaaacta aaacgacaaa
    19021 aggaatctct tctggttgct aactaggagg gacatagatg ctaaaagctt ttaaattccg
    19081 tatgtatccg acagaagaac aaaaacagca attgattcgt acttttggat gtgctcgatt
    19141 tacctataac catttgctga aaaagcggca gaaaagttgg caacaaacag gagtagccaa
    19201 tttttcgctc acacccgcta cattaaaaaa agaataccca ttcttaaaag aagtagacag
    19261 tcttgcttta gcaaatgcac agcttaattt ggatcgggct tttcgcaatt atttcaaggg
    19321 acgtgccagc ttcccaaagc ttaaaacgaa aaaaagtatg tggcaatctt atacgaccaa
    19381 caaccaaacc cgtacgatct atctggaaaa tggctactta aagttaccaa aacaaaaaga
    19441 actaatcaag ataaatcagc atcggccagt tgaaggatcg attcgctccg ctacgatttc
    19501 tgctcgatat aatgaagaat tttatgtcgc actactttgt gatgtttcgc cagtaaaaaa
    19561 agaatcttta gctaaatgga tcgggatcgc ctatcatccg aagacattga tccaaacatc
    19621 tcgacctcta gaagtaactt taccaaaatt tcaccagaca gaagaaaagc tgcaacatgc
    19681 acagcggaaa ttgaatgtga aagtccgctc cgctcaccat agaaagatca ggttagatca
    19741 ggcaagcaat tatcagaaac aaaagagaaa agtaatggat ctttatctga aacaaaagaa
    19801 ccagcgagaa gattatcttg aacaattatc tgggaaattg gtcaaacaat atgactactt
    19861 atttgtagaa tcttttccaa aagaagaggc acatgctgat ttttctattc acgactggca
    19921 taaattaata acaaaattgc gttataagtc ccaatggtat aataaaaaat ttcttttgat
    19981 caataccgac ggagcagaag aaagcaattc cgtaagaaag agccaagtag tagaaaaaat
    20041 gggcagacat tcggttatca agggataaaa aatgaatgaa actgatagga gcgttagggt
    20101 tagccttgtt aataaaaagt cttaatgact tttgtttcta ggaatttttc ttttgaagaa
    20161 aagctcacta attatgacaa accaataaaa caatagcaga aaaagttcgt acgagctttt
    20221 tctgctattg ttaatttttt attcagaaag tttacgaata catcaaaatt ctgaaacaaa
    20281 aaacgtaact cacaaagagt tgtttttgcg ttttttcttg aatgattagg tctgaatctt
    20341 ttgctcacaa ccttattcat ggtgccccgg cttgagctga tagatttttg aaggacggcc
    20401 gattcctaca ggacgttcac cgatctcatc gaaatattgg agcatagctt tcttaaaatt
    20461 ggaatgatcg atcgttttat aatcgatccc taagaattta gcaaatactt ttcgagcttc
    20521 cgtaatcgtg aaatctttac ctaaaacttg tagaacttgc ggctcgtgtt ccattttatt
    20581 gactacacgg ttgaatgctt tcaagatgat ttgactatgg tcaaaagcca aagtatcttt
    20641 gccagaagac tctccagtga ctaaatcaag cgagatagcc acttctccgc tagataaatg
    20701 gatcttgtta ctatgacgtt ctagtgtgaa ccagcgtact tccttggcat catctccggc
    20761 gattagcggt tcttctccaa taaaggctaa gtagctgact gtgacgaccc agcctcgcgg
    20821 atctcgatta ggcgtactga atgtatgcag ttgctcaata ttttcttttg aaataaccac
    20881 accagtttct tcctttgttt ctcttaagac gctttctcca gttgactcat ccttttggac
    20941 aaagccacca ggcaatgccc atgaatttcg ataaggatgc ccttttcttt ggatcaacaa
    21001 aactttcagc tgatcttctt ctttgttata gcacatcagt acgatatcga cagtcaaaga
    21061 gggcttgtca tattctggac gttcctgttt ttgataccag ttaagaaatt cttcttcact
    21121 tgctttatgt tcataatata atttttcttc ttctttagaa gcaaaacggt tcatctgacc
    21181 acctccattg acagtaaaag tactttttac cttattgtag cagactctga aaaaaaacca
    21241 aggatttact tgaaaaatct aggttatcat ttaaatatga aaaataaatg gaagtatgag
    21301 attgaataaa aagaaaagca gataaaacgg caatacttta taccgctctt cacgaatggc
    21361 aaagaagcca caaataacga tatatacgtc aatgataaaa cgcaaaatcc aagctgctgt
    21421 cataaaactg ttcaaatgcc catggaggtt aaaataacca tacatacgaa tataaaaata
    21481 gctgaatatt aaatgtgcta atgaaagaaa agcaatacta agtgtaaaaa aaagatgttt
    21541 cttctgcatg attgttccag tcccttacta agatagatag tctttattat accacgtgtg
    21601 aggggagaaa taaaaagccg ttttttagta gatatgaaac aaaacaaaaa cccgcattta
    21661 ttcaacctat tcatcctttt aaaggataga ttgaacgtag atagaagtcg tttatccagt
    21721 ctttttcatt ttgaaaaaaa gctaacagca tgcttttttt atggtatact agcaaggaaa
    21781 cgaattcgca tcatgcgata aaggaggaag agccccgtgg cagaaaagga aactttttat
    21841 atcactacgc caatctatta tccaagcggc aaacttcata tcggcaactc gtatactacg
    21901 attgcatgtg acgcaattgc tcgttataaa cggttgatgg gctttgatgt gttttatctc
    21961 actggagtgg atgaacatgg tcaaaaaatc gaaacaaaag cagaagaact aggtgtaaaa
    22021 ccgcaagaat atgtagataa aatggcggct gatgtcaaga aattatggaa aacgcttgat
    22081 atcagttatg ataaattcat tcgcacgaca gatgattacc ataaagcagc tgttcaaaaa
    22141 atatttgatc gtttattaga acagggagat atttacttag gtgaatatga aggctggtac
    22201 tctgtttctg acgaagaatt ctttacagaa acacaacttg cagaagtgta caaagatgac
    22261 gaaggtaacg tgatcggtgg taaagcgcca agtggacacg aagtagaatt ggtaaaagaa
    22321 gaatcatact tcttccgcat gagcaaatac gcagaccgtc tattggaata ctatgaagaa
    22381 catcccgaat tcatccagcc agaatctcgt aaaaacgaaa tgatcaacaa ctttatcaaa
    22441 ccaggattag aggatcttgc agtttcacga acaactttta cttggggagt accagtcaag
    22501 agtgatccga aacatgtggt gtatgtatgg atcgatgcat tatccaatta tattactgca
    22561 ctaggatatg gctccgaaga tgaaagtcta ttcgaaaaat actggcctgc agatgttcag
    22621 atggtcggta aagaaatcgt tcgctttcat acgatctatt ggccaatcat gctgatggcc
    22681 cttgatttgc ctctacctaa aaaagtattt gggcatggct ggctattgat gaaagacgga
    22741 aaaatgtcta aatcaaaagg aaatgtcgtt tatcctgaaa tgcttgtgga acgttatggt
    22801 ttagatgcac tccgttatta tttgcttcgt gctgttccgt ttgggtcaga tggtgtcttt
    22861 acgccagaag attttgtttc ccgtgtcaat tatgatctgg caaatgatct agggaactta
    22921 ttgaatcgaa cgattgctat gatcaacaaa tattgtgatg gactcgttcc tgactatgcc
    22981 tcacttgtca ctccatttga tagtgaactg tctacgacgg ctgctaacgt tgtcggacgt
    23041 taccatgatg caatggaaaa aatggaattc aatacagcat tggctgaaat ctgggtgctg
    23101 atttctcgtg caaacaaata cattgatgaa acagaaccat gggtattagc aaaagacgaa
    23161 gaaaagaaag ctgaattgaa tagtgtcatg atccatttag ctgaatcttt gcgtatcgca
    23221 gcgatattac ttcagccaat catgacagaa acgccagaga agatcttcaa tcaactagga
    23281 ttggactctg aaacaatgaa tcttgaagga ttacactttg gcgaatttcc ttcaggaaca
    23341 aaagtagttg caaaaggtac accaatcttc ccacgtttag atatggaaga agaagttgct
    23401 tttattcaag aaaaaatgtc tgaaggtact caaacaaacg aagatacagt caaatgggat
    23461 ccagaagaaa cagaacttgt ttcaacgaaa gaaaaacaaa tcaaatttga tgtatttgaa
    23521 aaagtagaac taaaagtagc agaagtcatc aactgtcaaa aagtagaagg ggcagataag
    23581 ttgctgcaat tccgactaga tgctggagac agccaagacc gtcaaatctt gtcaggaatc
    23641 gctgaatttt atcctgatcc aagcgaattg atcgggaaaa aagtcgtcat tgtagcaaat
    23701 ctgaaaccac gaaagatgcg tggacaaatc agtcaaggaa tgatcctttc tgcagaagca
    23761 ccagatggct ctttacaagt aatcgaagca ccaaaatcca tgccaaatgg ctcagaaatc
    23821 gcatagaaat tcaaaggggg tatgacacta gttgtgtcat accccctttt tccgaaattt
    23881 gagaagcaat tttcgtaaat tccttcttat tacttgaagc caatcatata atacagaaac
    23941 agagaactat ccaaaataaa tcctagaaaa agaagtacga gcttcgctga tttttgttaa
    24001 tttagaataa tactaatagg aatcgtaaaa acagaaaaaa agcataatga taaagggtcg
    24061 ggcagatttt aattgattca tctggtactc ttttttattg tccttacatt cataaagttt
    24121 gaatgcaatg actttattac atctatattt atatctaaca gtagcgttga tgtgttaatt
    24181 ataagttcta tcactttatg ttcaattagt ttgtgctctt atatgtgtga ttgaaaatat
    24241 agataaaaaa acaatcatcg gctttgactt cgatgattgt tttctttatt ttgtaaaaat
    24301 cctatcactt gattttatgg taacctttta tttttgtctt caaacggctt ggtaagagag
    24361 cagtaagcag tgcaaatggg atcgaaaaga cataccagaa aatcgtacgt ccgataccat
    24421 aatgttgata gagctttgtc aacatcacgt gtaacaagat caaccctaaa agcaaggagc
    24481 cagctataaa ccaatgatta tcccaatgca tgacttcact aagtgccatc actccaaaag
    24541 gatagaaata aaaatagatc ccgactaagg tcatccgcca gttctttgta aatgttccga
    24601 tattcaataa aaagaaaatc gctgaaagca aaataccgat aggcggaaaa agcccaacca
    24661 aaaaggaata caacatcccc cacaagatca tcacaggacc tttgatcaat gcaacgattg
    24721 cgatgaacaa agtcagcaat attagttttt gtgataacgt tgcaacagtc attaatccaa
    24781 ataaaattaa tgtgaaccaa tgccaatggg actgctgttg ttctttacgg gaaaaatcga
    24841 cccggttgga taaataatct gctaatttcg gataagaacg cttaaaatcc ataaactgtt
    24901 cctccttcgt ggaaaaggta ggtttagtat acgaaaaata aaaaagaaat acatccgtct
    24961 taagagggag tttttcactg aaagtggaga aaaaagaatg gtaaattaaa atattctcag
    25021 ttatactgaa aattatacca aatttcaaag aagaaagagg atgggaatga aaggcaaact
    25081 aaaacgagaa atcaatctgt ttggcgcatt agctaccgtt atgggaaccg tgatcggtgc
    25141 aggcgtcttc ttcaaaactg ctgctgttac tgctagtact cagtctacaa gtttgacatt
    25201 gctagcttgg cttttgggtg gtttcttaac gatctgtgct ggattgacag tggctgaatt
    25261 agctacagca atcccggaaa ctggtggagc tgtaaaatat attgaagctg cgtatggaaa
    25321 attgcccagc tttttattag gatgggcgca aagcttgatt tactttcctg ctaatattgc
    25381 tgcattatcg attatctttg cgacacaatt gacgaactta ttacaattat ctaccgatta
    25441 tcttttgttg attgcagtga tcaccgctgt ttctgtgaca gggttgaatt tgttagggac
    25501 aaaagtcggt gcttctgtac aatcagtgac gttgattgtt aaattaatac caattgctgt
    25561 gattgtcata tggggattat tgaccccagg acaaggaact gtccagttat ttccaatcga
    25621 agctggaaaa gatgttactt ttgtcgaagg attgagcagt gcgttgttag caacgttgtt
    25681 tgcttatgac ggctggttag gtgtcggtgc aatggcaggg gaaatgaaac ggccggaaaa
    25741 agatttgcct aaagcaatta ttcttggttt gagttttgta acagtcgttt atctattgat
    25801 caattttgtt tttttgaaaa ctttgccgat tgatcatctt gccggtaatt tgaatgcagc
    25861 ttctgaagca tcagacgtca tttttggagg aatcggtgga aagctggtca ctattgggat
    25921 cttgatctcg gtatacggtg cattgaacgg ttatacacta actgggatcc gcgtgcctta
    25981 tgcgatggct ttagaagacg agctaccttt cagtaaacag ctgacaaagc tttcaaagaa
    26041 gtttacggtt ccttatgttc ctgcggtttt ccagttagca gtagcttgta ttatgatgag
    26101 ccttggttct ttcgactttt taacagacat gttgattttt gtgatgtggc tattcagttt
    26161 actgattttt atcggtgtct ttattttaag aaaaaaagcg ccggaattgc cacgtccata
    26221 taaagtacca ctttatccaa tcgttccgat tattgcgatt ctcggtgcta ttttcatttt
    26281 aggtatgacg atgatgacgc aaacgaaatt agcattgatc ggtataggtg ttaccttgat
    26341 aggtatccca gtctattatc aaaaaaagaa aaaacgctca gaagagtaaa atatttcaac
    26401 aacagacaga aaaagtacga actaggtcct ctagatcttc gtgctttttc tatttcaata
    26461 aaacaagttc ttctattttg atttttgcta cttctgcaag tagtgcttat gtgctaaagt
    26521 aagaatgaaa aggagagata aacatgatat ttgattctca tacccattta aatgctgaac
    26581 agttcaatga agatattcca gaaacgattc agcgagcaca agaaatgggg gtaacgaaaa
    26641 tggcggtcgt gggcttcgat acgccaacga ttgaaaagtc tttgcaatta agccatgact
    26701 atccaaatat atacagtatt atcggctggc atccgacaga ggctggcagt tatacgaaag
    26761 aaatcgaaaa taaattacaa gaacaattga ctttaccaaa ggttgtagca ttaggggaaa
    26821 ttggcttgga ttatcattgg atggaagacc cgaaagaagt ccaagataaa gtatttcgca
    26881 gacagattgc tatcgcaaaa gaaatgaacc taccaatcag tattcatact agagaagcac
    26941 ttgaagacac gtatcaaatc ctaaaagaag aagatgttcg agatatcggt ggaatcatgc
    27001 atagtttcag tggtgattat gaatgggcaa aacgatttct tgacttaggg atgcatattt
    27061 catttagcgg tgtcgtcact ttcaaaaaag cattagacgt ccaagaggct gcagcaaatg
    27121 taccaatcga acgattactt gtggaaacag atgcccctta tctggcacca gttcctttta
    27181 gaggaaagcg aaatgaacca ggctatactc gttatacagt agaaaagatt gctgaacttc
    27241 ggaatcttcc gacagaagaa gtggctgccc aaacatggac aaatgcgcac cgattatttg
    27301 gtctaagcga acatggataa gatgaagatc caagagatta tagtcgttga aggaaaagac
    27361 gatacaagaa ggctgcggga agtagttgat gcagatacga tagaaacaat agggtctgca
    27421 atcaatgaag agatcctgat gcaaattgaa catgcccaag agactcgtgg agtgatcgtt
    27481 tttaccgatc ctgatttttc aggagaaaaa ataagaaaaa tcattatgga agctgttcct
    27541 caggcaaaac atgctttttt gccgcgcaca caagctaaac caaagaaaaa gggaagcctt
    27601 ggtgtcgagc atgcaagtga tgctgctatt ttagaagcat tgaaaaaggt cgtgacgcca
    27661 gacatttccc aaacggctgt ttctgaaatc actagacaag accttttaga ctatggttta
    27721 atagctgggg cacaagcaaa agaaaaacga gaaaaactag gtgacgagtt gcgcatcggt
    27781 tacacgaacg gcaaacaatt ggaaaaacgt ttgaagatgt ttcgtattac gccgaaagaa
    27841 ctcgcccaag caatgaaaat agtggaggaa caacatgagt gaatatcgtg aaattgcgac
    27901 accctctaga acaaaagaga ttttattgaa acatggtttt tcatttaaaa aaagtttagg
    27961 acaaaatttt ctgactgaac caaatatttt aaggaaaatc gtagaaactg cgcaaataga
    28021 tgaacatacg aacgtgatcg aagttggacc aggaatcggt gcattgacgg aacaattagc
    28081 caagcatgca aaacaagtcg tagcatttga gatcgatgat cgtttgatcc ctgtattggc
    28141 tgatacgatg caaccctatg acaatgtgac tattgtacat caagatattt taaagaccga
    28201 tttgtcaaca gctgttagag aaacatttca tgaggagctt ccgttaaaag tcgtagcgaa
    28261 tttaccttac tatattacga caccgatcat gatgcatttt cttgaatcag atttggttgt
    28321 ggatgaacta gtcgtcatga tgcaaaaaga agtagctgat cgtatctctg ctgaaccagg
    28381 aacgaaagct tatggttcat tatcgattgc tgttcaatat tatatggaag ccagtttagc
    28441 attcatcgta ccaaaaacag tatttgtccc tcagccaaat gtagattcag cgattttgaa
    28501 actgacaaga agagatatac cggcagtcga agtgacagac gaaaaagcat ttttccgatt
    28561 gacaaaagcg gcatttcagc aaagaagaaa aacgctttgg aataacttgc agcattcgta
    28621 tggcaaagat gaccagacaa aagcatggtt agcaaaaagc ttggaaacag ctggaatcga
    28681 tccaaaacgt cgtggggaga ctttgtcatt acaagaattc gccgcattaa gcaatgcgat
    28741 gtcagaaaat atgcagtaaa aaaagaggtt gtgacagaag ttgtgaaaaa gctgtcctgc
    28801 atcaagtaat aagaacagcc aaacaaaaat agttcctcat gtttttcgtt tgtttgggct
    28861 tattaccgca gatgcaagct tttgaacacc gtttaagaaa gaggttgtgg gcctgccgtt
    28921 taactctaag tcacaaggaa caatttcaaa aaacagcttc tcatgttttt gagttagttg
    28981 gtcttattac ttgatgcaag cttttgacca ctattatgcc gaacaaggag atgtgacaca
    29041 aattttgtca catctcctat ttttgtttct tgatcaaaaa attcttatac ttgatactca
    29101 cactcatttt gtatggattt agaaaagaat atgtgtcttg acagttatta gtaagtgagt
    29161 tatactaagt cgacatttat gtgtcaagac atattaggag agagaaaaaa tgagaaaaaa
    29221 ttcagttcga aaactaacga tttctgcttt attgattggg atgggggtca ttatcccaat
    29281 ggtcatgcct aaaatcatga ttccaccagc ttcctttact ttggcgtctc atgtgccatt
    29341 atttattgcg atgtttttta caccaggagt tgcagtggct gttgcgctag gtacgacatt
    29401 tggtttcttt ttaacaacgc cggttatcat cgcattgcga gcattgtcac atcttgtgtt
    29461 tgcgttgatt ggggcttggt acttgcaaaa gcatccgagt atcgttttaa aaaatggaca
    29521 gtttactttt gctaattggc gtttccaagg gttcaatttt gtgatcggtg tgattcattc
    29581 tttagcagaa atgctagtag tcagcatctt ttactttatt ggtaatatgc ctgaaacata
    29641 ttattctcaa ggatattttt atacagtatt catcttgatg gggattggtg ggcttatcca
    29701 cagtttagtt gattataata ttgcttattt tatagcagga acactcagca aatattttga
    29761 tatccccgta tttacggcag caaaaaaaga aaatcggaaa gagtcgattc aagggtacgc
    29821 gaagaaaacg acatattaga catgatctct caaaaaaaaa actctcatct agtaatggat
    29881 aataaatcct ctaagatgag agttttttgt cctaaaaaga gctgcaccaa catcttctgg
    29941 cacagctcta acagggggca atttatggaa gtttgatgac ttgttctgtt tctttatcaa
    30001 agaagtggcc tttgctgatg ttgaaggcaa ggtcgactat ttcgcctggt ttatggaaat
    30061 ctcttgcatc tacttttgag atgaactcag tagaaccagt ttttgtgtaa agcatcgttt
    30121 cagctccaag taattcggaa acgacaactt ctgatttcac tacggcttca ggtgaagctt
    30181 caagggctac ttgttcactg tggatgtctt ctggacgaat accgaagata acttcttttc
    30241 cttcataacc tttttcaact aatactttgt tttttccttc aggaatacga agcttaagcc
    30301 catgtccatc tgtgataaca ccatttttca atgtcacatt aaagaagttc atggcaggag
    30361 atccaataaa gccggcaacg aacatattga cgggtgtatc ataaacttct ttcggtgtac
    30421 cgatttgttg gataaatccg tctttcatga taacgattcg gtcagccata gtcattgctt
    30481 ctgtctgatc gtgagtaaca tagatggtcg ttgtttcaag acgctggtga agtttggcaa
    30541 tctcagcacg catggctaca cgtaatttag catctaaatt tgataatggc tcatccatta
    30601 agaagacttt tgcatcccga acgattgccc gtcccaatgc cacacgttgg cgttgtccac
    30661 cagataaagc agctggtttt cgtttcaagt attctgtcag acctaagatc tctgctgcat
    30721 tgtccacacg ttttttgatg tctgctttat catatttacg taatttcaag ccaaaagcca
    30781 tattatcaaa aacagtcata tgaggataca atgcgtagtt ttggaaaacc atggcaatat
    30841 cacggtcttt aggtgccacg tcattcatga cttttccgcc gatatttagt tctccttcag
    30901 agatatcttc cagtcccgcg atcattcgca aagtagttga ctttccacaa ccggaaggac
    30961 caacaaaaac gataaattca cggtctttga ttgatagatt aaaatcagtt acagaataat
    31021 tttctgcatt gtcatatttt ttatatacgt ttttcagagc catttctacc ataatacgtt
    31081 ccacactttc ttttttagtt ttattaacta tttaaagtat aaatgaaagc gttaactaac
    31141 ttcaatgtca tgttgaacaa gaattttttc ttttttttgt caatgtgcta gttttaagct
    31201 ttaataaata tccataagaa ataaaaactg atacactgga gtaaataagt atgttaacaa
    31261 tatcaaaagg agtgtctctt atgaaaatcg ctttgatcgc tcatgaccga aaaaaaccta
    31321 tgatggtgaa attagcaaca gcgtataaaa caatattgga aaaacatgaa ctttttgcta
    31381 ccgggacgac tgggcagagg atcacggaag caaccggctt gccgatccat cggtttaaat
    31441 ccggaccgtt aggcggggat cagcaaattg gagccatgat ttcggaggat cagttagatt
    31501 tggtgatttt tcttcgagac ccgctggctg cacagcccca tgaaccagat gtaacagcac
    31561 ttatccgttt atctgatgtc tatgagattc cgttagctac gaatatcgga acagctgagg
    31621 tgctactaag aggactggaa gccggcttcc tagattttcg aagaatcgtc catgaaatag
    31681 acaagcatcc actttcattt tgatggataa gcgtatggaa aaataacgtt taatgttcgc
    31741 ctatatgtag ctataaaagc taaacaaact gataatatgc gatataatac gaacgtattg
    31801 gataatataa acaataatat tagtttttcg tatttgcagt aaatgtgaaa atatgctata
    31861 gtttctgtgg gaaaagatcc caaaaataat acagagtaga aaataaagcg ttaagtgcta
    31921 agaggatggg atgttgcctc ttggacgaag gacgtaatgt tcgcggtttt attttcgcat
    31981 tcgctgcatg atacgatgta gcagctctta aaggagatgc taaaaatgaa gaaacaccgg
    32041 ttagacgcac gtatgattgc tattatgggc ttattgattg cattgatggt cactttgtcc
    32101 cgactggtag caatcgagac cccatttatt aaaatcagtg tcacatttat tccacaagtg
    32161 atcatgggca ttctttttgg tcctttttgg tcagggatag gcgcagtttt agctgattta
    32221 gtaggaatgg ccctcttctc gaaatctgca ttctttattg gatttacatt gaatgcattt
    32281 attgaaggag caatctacgg attctttttc tatcgaaaag agatcacttg gaaaaatgcc
    32341 attctggcta cgcttagtgt cacgctgatc atcaacttat ttttgacacc attatggctt
    32401 gcgttgatgt accatgtgcc attgttcagc tgggtcgttt gggcgccccg cctgttaaaa
    32461 acagtaatat ggttaccgat tcaatcaatt gccatttatt acgtaggacg tagtattcct
    32521 tacaaaaaaa ttttaagaag tttagcgatc cacgctaaat aaaaaacaag cagtcttccc
    32581 atcccagaag actgcttgtt tttattgtgc tgtgaattgt gcttgataga gccgtcggta
    32641 ataaccatct ttttgaagca gctcatcatg ggtgcccatt tcgacaatag ctccctgatc
    32701 catcactaat atctggtccg catttttgat tgtggacagg cgatgggcaa tcacaaaact
    32761 ggtttttcct tccatcattg ctaagaaggc ttgttggatt tttttctctg tcaatgtatc
    32821 gactgagctg gtagcttcat ctaagatcaa cataggggga ttactgatca tcgttcgagc
    32881 aatcgtcaac agttggcgtt gtccatctga tatctttaac ccttgaccgc caatcttagt
    32941 atctaatttt tccggcaaac gttctacaaa ttcatacata taagaggctt ttagggcatc
    33001 attgatttct gcgtcacttg cttccggatt cccaaaacga agattttctc gcaatgtact
    33061 gtcaaatagc caagtatctt gtaacaccat tccaaaactt ttacgtaatg tatcacgttg
    33121 gatagagcgg atgtcatggt catcgactgt aattgttcct tttgttgtgt cataaaaacg
    33181 catcagcaaa ttaacaagtg tggattttcc ggcccctgtt tttcctacta tagcaatggt
    33241 ttgacctgga taagcagtaa aattgaaatc ttcgatcaaa cgctgcttgg ggtcataaga
    33301 aaaagaaaca tgatcaaaag agacttttcc tttgatattt ttgggttcca aggcataggc
    33361 atctggcaaa tcaggctttt cttctggctg atcgatcaaa tcaaatgctc gttctagacc
    33421 agaaaaagcg gtttgcagtt gtgtcgtgat tccagaaagt tcgataaatg gtttagaaaa
    33481 ctgacttgag tagatcgtaa agctggaaat cacaccaaca gtgattgacg aaccactctt
    33541 caatgccaat aggccgccaa ctaaaccaat cgataaataa gcaaggtgat ctacaaaacg
    33601 agaagacggg tttgtcaagg aagaagaaaa ttgtgcccgt tgtccttttt catatagatc
    33661 agcatttagc agttcaaatt gctgttgact agcttcttct tgctgaaaag ctttcacgat
    33721 tttctggtta ccgattcgtt cggtcacaaa gcccgagata tccccaataa tcctttgctg
    33781 tctatcgaaa tttgtttgtg aagcacgagc aacgatccaa ctcacaagga aaataattgg
    33841 tgtagaacaa agaacgacca atgtcagaag tggacttaga cgcatcataa aaataaacga
    33901 aagaatgatg attgccactc cagaaaataa ttgattgaag gccgcagaaa ttgcaatgga
    33961 aatattgtcc atatcatttg taaatcgact gacgatattt ccgtgggagt tttgatcata
    34021 ataacgaagt ggcaatcgat tcagatgagc aaatgcatct tttcttaatt ttgcaacgga
    34081 taagtaagct aaccgattgc ttaacacttg gatgagccat tgactgacta cgttgattaa
    34141 aacgatacca ccgaatagaa taagtaaatg gaacagtcga ttgaagcgga cctgtccttc
    34201 tccaatcatt tgatcgatac tttgaccaat ttgtaacgtc atcacaactg ttgcaatccc
    34261 attgatgatt cctagtatca cagctagcca aatgtctttt gcataggcag taaggtaagg
    34321 aatgaatcgg cggagcgtct gcaaggataa tttttttact ttcataacta ttcttcctcc
    34381 tgtgatgcga cgatttcctg atattctgct gattgagcaa gtaattcttc atgggtaccc
    34441 tgagcagcaa tcttaccttg atccatcact aagatatgat gtgcttcttg aatagagcgg
    34501 acacgttggg agataataat cactgtactg ccaagataag tctgtagcgc cttacgcaaa
    34561 tccaagtctg tttgataatc caacgcactt aaagagtcat ctaaaatcag taatgctggc
    34621 ttggcaatca aagctcttgc aatcgtcagt cgctgttttt gtccgccaga gaaatttttc
    34681 ccattttcca tgacttttgt atctaaacca tttggaagct gacggacaaa ttcttctgcc
    34741 tgagcgattt tcagagcttc ccaacattct ttttctgtgg cgtctttctt accccattga
    34801 aggttttcac gaatagtccc ggtaaatagt acagaagttt ggggaacaag agcaatttgc
    34861 tgacggagat gatccagctg ccaagaacga acatccattc cgtccattat aactgtaccg
    34921 gaactgacat catagaaacg cggaatcaat tgtgtcaaag tcgttttccc gcttccggtt
    34981 ggaccaatga ttccaaggac tgtatttcgc ttcagctgaa atgtaatatt ttggagagaa
    35041 agaccagaat caggagtata acggaagtca acatggtcaa aagcgataat tgcttcagaa
    35101 gaaaaatcgg gagtagtact gacctcagct tcaagaatcg aattttctgt gtctagcacc
    35161 tctttcaccc gatttcctga agcttcggca cgtgtgaaga tcacgacgag gtttgaaaca
    35221 acgatcagtg ccaaaagcat ttgattcata taattgatca aagcaagaac ttgtccttgt
    35281 tgcaagctac caatatttac tttgatcccg ccaactgtca gcaaacagat gatcccgaca
    35341 ttcatgatca aggttgtagc aggtgataat agggcagaga tattggaaac acgtaaataa
    35401 ttgttcgcta gatcatttgt gcttttatcg aacttctctg tttctttttt tgtctgcgca
    35461 aatgcgcgga tgacacgaac accgctaaga ttctgactaa cgtgttcatt gagctgatct
    35521 agtttttctt gcactttttg gtagagagga acggtttttt tgatgataaa aaataaaatg
    35581 attgagaaaa taggcaataa tgctaagaaa aacagaccta cttcccagtc gatcacaaaa
    35641 gccatgacca ctgacccgat acttaaaaac ggtgctctga tcaataaacg aatgaccatt
    35701 gctaaagcta gctgaagctg attgatgtcg tttgtcattc gggtgatcaa ggtatctgtt
    35761 ccaaaatgat tcaactcgtt gtgagagaaa gtattgattt ttttgattag ctgattacgt
    35821 aactctgtac caaatccttg tgaagctact gaagcatagt attggcaaat gatagcacaa
    35881 atcagaccaa tcacagacat agcgaccatc cataagccca tttcaatgat ttttcctcgg
    35941 tcattcattt ttagtccatt atcgatcaag gaagccatca ttaaaggcag gacaagttcg
    36001 aagacagctt caagaaattt gaagaacggg cccaaaatga tttgcttacg atagtctttt
    36061 gcataacgta ataactgaat catatgattc ctcctattat ttatttgtcc acaattgaaa
    36121 aaacaagtac taccacattg tagcttagga aatttagaat gaaaagagaa caagcctttt
    36181 cataaatgaa agttttatta tgaagaagct ttagaaattg aatagactat tttttttaaa
    36241 aaaaagaaaa tgagaaaaca tgccagtctc ttcattcata ggaacgtcta ttttacttaa
    36301 tttttctata aatgaattct gcataaaaaa tatatcaagt aaatgtttat agatgaaact
    36361 ctatttactt cttttttaat ttgttcttgc aatctttttt gctacgtaat ttttgtaaaa
    36421 aagtgggttg agagaaatct aattgatttt caaagaagga gtatgaaaat gagaaaaata
    36481 tttattcgtt tattgtgggt actatcttag aggaataaat aatagtggag aaaactatgg
    36541 tttggcgcag attctatcag gtttgatgga acgaagacaa cctcccgaca tcaagtcaaa
    36601 agctacttga gattttgatc tgaaaataaa aaaagaaaac aaggtaggaa catcaatgtt
    36661 tctaccttgt tttcgtaatc aatgggaaaa aactaagagt ggtgtacgta atcaataaaa
    36721 aggatggtga caacagtacc tctttactct ctgaaagcat cataactcgt ttttcctcat
    36781 tatgccaata aattgttgtt ataccacata taaggatttg taatagctaa aaacagacag
    36841 gaaagttgaa atctgtgaat cttattttgt atggaaggat ttatagaacg caaaaatcaa
    36901 gataaagatg aataaaaatc ctgtttcggc tatatattgc gtatctacgc caataaaacg
    36961 agtttgttct gtcatagcca tataaagcaa aggcaaaaat acaactacag cgagataaga
    37021 tccgatgatt tctcgaaaag ttttccagga agtatgtttc ctgtttttat atttatccga
    37081 tttcattgaa tagtcatagc ctccttattc tttcatatgt tgaaattcca gtataaaaac
    37141 tggaaggaac tgtacgtttt ttagaataaa aattataagg gataagtagg ctgctgtcta
    37201 taataagata tatttttcaa ttctttatta aagattttat tgacaaaatg aatccgtttt
    37261 ctatgatgga ggaggggtga ataagttgtt gaagaaggaa attttatgtt acttagagaa
    37321 ccagacagcc tttttcgatt tagaacatat gaacgaggtc tttacagcac aaaggttagc
    37381 tgatcatttt ggggtaaaaa gaaacacagt cagtcattat ttgaaccagt taaatgatga
    37441 gcaactacta gtcaaaatca attctcgacc agtgatttat ttccacaaag aagcttttga
    37501 aaaacaattt tttaagttac gcacgaattt ttatttttct atcgatgatc tcaaagagga
    37561 acagcctttt ttcgcgcaac caaaagactt attctctttt atgatcggat acgacgccag
    37621 tttgaaagaa agtatcgaac aaatcaaaac agctctttac tatccagatg gcggattacc
    37681 acttttgatt acaggcgaaa gtggcacagg taaaagttat ttagttaatt tggtctatca
    37741 gtattgtctg cttcatgatc tgctagaaga ttctgcacca ttcatcactt ttaattgtgc
    37801 acaatatgca gataatcctg aacttttgac tagcaatttg tttggacatg ttaagggagc
    37861 gtacactggt gcagaagaaa ataaaaaagg ggcttttgaa gcagcagatg gtgggatctt
    37921 gtttttagac gaggtacatc gcttaacacc agaaggtcag gaaaaactgt tcacctatct
    37981 tgatcaaggt gtgatttatc gtatgggaga acctaatttg tcacgccgga tcaaaacacg
    38041 tctctttttt gctacgactg aagagattac tagtcatttc ttaacaactt tcattcgaag
    38101 aatacctatc cgaatcgaac tacctgcact taatcagcgt agtcgtaatg aacggctaga
    38161 actgatttac tctttttttc tcgaagaaca aagaaaaatg gggagccctt tgtcagtcag
    38221 tggaccagct ttgtcacttt taacaagtca gaaattatcc ggaaacatag gagagctgaa
    38281 aaatatcgtc aaagtttcag cagcaaaagc atatgctgaa cagcgtgaac agcctgaaat
    38341 ccatgtcacg atccatcatt tgcaaaaaga actacttgcg cagcctatta ctaaaaatac
    38401 agcacagcaa ggaatctata ttacgcaaga ccagacctta gagcaattaa tggagaagca
    38461 acagcccgaa cagcagcgaa tcgttaaaag ttttgagcaa atcctaattg atttcaagaa
    38521 aaataactgt ctgctatatt cttgcgaagg aaaattaaag caagaagtga ttcaattatt
    38581 tgattttttg ctgtttgaga ccagtagaca agaaaaacac gagctgctgg tttattggac
    38641 acaatctatc cgagagacct ttcgacaaat ggaaactgcc tatcaaatca aattcgatgg
    38701 aaacagtgtc tatgccttga gttactatct ctatcagcga agcactatca aatggttacc
    38761 ggaagaacat gatattatcc aactaatcaa agaattagaa caacaagttt ctcagtctta
    38821 tccatctagt tatcactatg tccaacgaat tctggaatta agtaagatca aattagatat
    38881 tgaagacaca agtatggatc ggattttact gacgatctat ctaaaaaaag cgaaatggtc
    38941 aaaagaaaaa cgactgccaa aagcgatgat tgctgctcac ggttatgcaa ctgctagcag
    39001 tatggcaaat gtagtaaatc gtttattaca agaggatcta tttgaatcat ttgatatgcc
    39061 actagatgtc acaccacagc agattgcttc agaaatcctt gattattgcg aaaacagcga
    39121 tgtttcaaat ggattgatca tattagtaga catgggatct ctgaaagaaa tccatcagtt
    39181 ttttaaaaaa cagttatccg taccgctttt gattttgaat aatgtgacga cacctttagc
    39241 aatcacagta ggggaatgcc tgcaaaaaaa tgtatcatta gaagagatag cagaagagac
    39301 tgttcgacag atccagccag aatggcgact gctctatcca gaggaaaaca aacccaaagc
    39361 actcattact acttgcttca ctggaatcgg tacggcggtc cacttaagtg aactgctaga
    39421 aaaaagctta cctacggctt gtcagttgaa aatcatccca tatgaatatc agcaattaaa
    39481 agataagaaa aacagtgacc cattattttc catatacgaa gtcgtaggaa tgattggcac
    39541 cacagatcct gatatcacca atatcgacta tctttcatta gaagacctca tctcggggga
    39601 aaagatgtct gtactagctg aatggttgga cactactatg aatgcttcag agaaggaaat
    39661 attcaatcag cgcatcattc gtaatttttc attggaaaaa gtactggatt ccgtgactat
    39721 tctagataca gagaaggtga tgggagaaat cgatcatttt atgagagaat tagaggtgca
    39781 gttgaatcga agcatcagca atcccaaaaa attagcgttg tatgtccatg tgagctgtct
    39841 cattgaacga ttgatccgac aaatgccaat cgaaacctat caaggattag agaaatggaa
    39901 gcagtgtcaa aaagaagggt tatctgctat taaatctgcg tttagtgtca ttgaagagca
    39961 ttatagtgtc atgatcccca attctgagat tgcctatatc tatgatattt tgagtgaaga
    40021 tacagagttt ctctcgatag aagaagaatt ttgaacataa gcaagttgtt gccactaatt
    40081 ttggcatgca acttgctttt ttagtatgtg taagtgaaaa gaggagatga aaaagtggat
    40141 agagaaatta tcttagcttc ccatggaaaa tttgcttcag ggatattaga ttcattagag
    40201 ctgatctatg ggaaaaatca ttctattact gtattcgatt gctatactga tgagaatttt
    40261 gatttagcgg aaacagtaag gcagcttttt atgaggtaca aagataaaga aatcattgtt
    40321 ctgacggatc tttttggcgg aagtgtcaac aacgaattct tacaatatat caatcgggat
    40381 catgtctatc tcgttgccgg attgaatttg ccactattga ttgagctagt atctcggttg
    40441 gacgctgaag tagagacaac tgttttgatc cagcaggtgc taactgattc aaaaaaaatg
    40501 atccagtttt gtaacgagcg gctcaatcag aaaatagaag aagacgaatt ttaggaggaa
    40561 gaaaaatgat tgtattgaca agagtcgacc atcggttatt acacggacaa gtagcttttt
    40621 catggacaca aacaataggc gcagactgta ttttgattgc taatgatgat gtacctacga
    40681 atgagatacg aaagacaacg atcaaattag ccaagccaca aggagtcaaa ctagtgatca
    40741 aatcaattga tgactcgatt gccgcgttga aaagcggggt gacagataaa tataagttat
    40801 tcatcgtagt ggaatcgatc gaggatgcat ataagctggc agaagcgtat ccagaaatca
    40861 aaaccatcaa cttaggaggg atcaaggcaa aacctggtac tcgcagcatc ggaaaagcgg
    40921 taaatatttt accagaagag gaggaacgct tgaaacgatt aattgacaaa caaatagaag
    40981 tagaaatccg tcaagtacca gcagataaaa aagtggatgt aagagaagtt ttgtaagaaa
    41041 ggaagaacct caatgattgt acaagcagtc ttattaggaa ttgttgcatt tgtcgcacaa
    41101 tcagaatatg cactagggac atcgttgctt tctcgtccga ttgtgacggg gctgtttaca
    41161 ggaatcgtgt taggtgatgt aaaaactggg atcattatgg gcgcaacttt agagttagca
    41221 tttatcggat cgttctcagt aggtgcttcg attccacctg atgttgtgac cggtgggatt
    41281 ttagggacgg cttttgctat tacagctgga gccggtacag aaaccgcgct tttactaggt
    41341 ttgccgattg ctacgttgac attgatattg aaaaatattt acttagggct tttgattcca
    41401 attatgaacc ataaagcaga caattatgca gaagaaggaa attataaagg agtcgagcat
    41461 atgcacttgc ttgccggtat cggtctttcc ttgatgcttg gtgttgtggt aatggtttct
    41521 tatatggtgg gaagcaatac gatcggaaat ctattaaata tgattcctga cttcgttcaa
    41581 aaggggttgt cagtagctac agggttgatt ccagctcttg gttttgcgat gcttgctcgt
    41641 ttgctgatca acaaacaagt tgcaccatat tttttcttag gttttgctat agcttcttac
    41701 ttagagattc ctgtgaccgg agtagctatt tttggtgcga ttttagcagt tgtggtagtc
    41761 aatattatga atgtccgtca actaccactt caaacaactg caggggaggt agaagaagat
    41821 gaagaattct gagatcaaaa acagcaaaat aacgaaaaaa gagctaaatc acgtattttg
    41881 gcgttctttt caaatggagt tttcttggaa ttatgaacgt cagatgaata tggcttatgt
    41941 ttatgcaatg attccgattt tgaaaaagtt atatcagtcg aaagaagaaa tggcagcagc
    42001 tttgaaacgg catctagagt tttttaacac gaccccacat atcgttacac tgattttagg
    42061 aatcacggct gcaatggagg aagaaaataa ggatgatcct gaatttgatg tgactgcgat
    42121 cgatagtatc aaaacatctt tgatgggacc tcttgcaggg ttaggagatt ctttcttttg
    42181 gggaactttg cgattgattg ccacaggtgt gggtacctct ttagccatgc aaggaaatat
    42241 tttaggccct atcttgttca ttttgatttt caatattccg cacattctgt ttcgctatct
    42301 agcaacaggt tggggatatc gcttaggaac gggttttttg aaaaagatcc aggcaaacgg
    42361 tatgatggaa agtttgacat taggcgcgtc tattattgga ctgatggtcg ttggagcaat
    42421 gactgccacg atgatcacca tcaatattcc tttaaaaatc ggttcaggag aaaatgctgt
    42481 gactgttcaa agtatttttg atgatatcgt tccaaatatc ttatgtcttg gagcatttgg
    42541 catagtgttt tatctattga agaaagaagt gaaaccatta acgatcttga tcggactagc
    42601 tttctttgga atttttggtt cgtggatagg gattttttaa aaaaggagca gagaaaatgt
    42661 ttacgatgca agattatatc tatgaagaaa aggaagtact aagtacgatc ttgaaaaaga
    42721 atgatttttc gacaagagaa catatgaaaa aaacagtgaa tttactgatc ttagcaacgg
    42781 gttcatctta taacgcttgt ttagcagcga agccagcatt ggaatcttat ggtgatctta
    42841 ctgtcgatat tcaagaacct ttttattttt tcaattatgg aaaactttct ccttcaatcg
    42901 atactgtgat tgctgtgtca caaagcggga aaagtgcttc tactgttgaa gcagtaaaaa
    42961 tgatacaaaa acaagggtta cctgttgttg ctatcacaaa tgacgtgcaa agtccacttg
    43021 ctttagaagc tgcacaaatt attgatttag gtgccggggt agaatcagta ggcttcgtta
    43081 caaaaggcta ttctgcgact gttttacagt tactactctt agggatagga attggaataa
    43141 gtaaaaataa aatcagcaaa aaaatagaac aagactacat acaacagttg cgcaaaataa
    43201 tcaaccattt accagcgatt attcaaaaaa cggaagaatt ttttgatgga tatcaaagtt
    43261 tatttcgttt agcacagcgt ttcgtcacca ttggatatgg tccaaactgg ggaacagcta
    43321 aagaagcaga gacgaaattg actgaaacaa tccgcgttcc gtcacaagga tttgagttag
    43381 aagcttatat gcacggccca tacctggagg cagatgcttc gcatttgcta ttctttattg
    43441 aaggggaaag tgtaaataag gaacgttcgc aaaagttgca aagatatatg agtcggtacg
    43501 ttggagagac tctcaccatt accacaaaaa aagcaagaaa cgagaaaaca cttggattag
    43561 caatagaatg tgatgaatat ctgtctgtat tagcgcttgt cgttccgttt caactattcg
    43621 cttataaaac cgcagtagct aaaggtattg acttgaacaa aaagattttt gaagactttg
    43681 atacagtctt gaaaagtaaa ttataagagg tggaaatcat gctgaaattc aatgaagaag
    43741 aacaaatcaa agcaataaaa ggcgcactag ctttacgtcc gcaagtagaa gaaatcatcg
    43801 ataaacttta tgcggaaaaa tttgacgctg tctattatct aggaatcggt ggaacgtatg
    43861 cttcctcgat gcaagctgtc acttatatga atgggaaaag caacttgcct gtctttgtac
    43921 agcatgctgc agaatattac actactggaa acaaaagatt aacaaaagat tctatcgtta
    43981 ttttgtcctc tgttaccggt acaactcaag aagtagtgaa agcagtagaa caaatcaaga
    44041 aagtaggagc gacactgatt ggatttatcg ataaagcgaa cagtaagttg tctcagctat
    44101 gcgattttgt ggtcacttat ccagctcctg gaacagaaca aatcaaattt tttatggcag
    44161 cagatcgatt gatgtatttg aatggagaat tcgaagctta ttcagaatac tatgaacagc
    44221 tcgaacgtta tttaccaaca gggttagtgg aagcagagaa aaaagcagat gcatttggct
    44281 tatcatttgc agaaaaacat cgacgtgata gtatgcatta ttttattgga gcagggaatc
    44341 aatggggagc tgtttattca tatgccatgt gttactggga agaacaaagc tggcttccat
    44401 cgaaatctat ccatgctgcc gagtttcttc acgggacgtt agagatcgta gaagaaacaa
    44461 ccccagtcac attattttta ggcgaagatg aacagagagt gttatctgaa cgtgtggcta
    44521 aacttttgcc aaaaatttgc agcaattata cattgataga cacaaaagac tatccagtag
    44581 aaggtatcag tgaaaaatat cgcggacgtg ttttgtcttt cttattgatg catgtagtga
    44641 cacaacgcat cgatgctcat gtggagcagt tgaactgcca tccattagag atacgccgct
    44701 actatcgtca gtttgattat taggatagac tggataaaaa gaatataaaa aataaagaga
    44761 gcagcgataa ctgctccctt tattttttta aaatattggc atcaattacg ttataaaaca
    44821 cattttttgc attatctaca gttcaactta taatctgttt aatgggacat gtgggtgcca
    44881 accatttttt gttatgcaat agtactattt agttgttgca tggatccgta gtaaaagtcc
    44941 aagtacaacg agttgcgcca gtttacatag cgttttgtat ccagaatgtt gactaaaaca
    45001 aaagttccta tgtcaaccgt caacagagac aatctgtccg ttgcaggacc tgacagattt
    45061 agaaatgttc aatctgtctc ttatgtattt taaatcaata ctcactagga gaagtttcct
    45121 aacctcctaa tagtaatgct ggtagtaaca ttgtcaaaac tgcttcctct taggaacaag
    45181 caaagcataa cgagtgtaaa aataaaatgt agactattgt aacatatcat tgattatttc
    45241 aagtaaaaac gataaaagaa gtaaacaaac acttaaaaac aatggaaatt agtcccaact
    45301 ttaagaaatg tatactataa tggaaattat caattattta tttggaggaa tatccatgaa
    45361 acaaaaaatc gcagttcttg gtcctggatc ttggggcact gctttagcac aaacattggc
    45421 agaaaacggg catgatgtcc gtatttgggg aaatgtacct gaacagattg atgaaatcaa
    45481 taagtatcat acaaatcagc attttttacc tgatttgacg atcccagaat cgattatcgg
    45541 atataaagaa ttggcagaag cagtggataa cgcagatgct attttatttg ttgttccaac
    45601 aaaagcgatt cgttcagtcg cacaggaact tattggtaaa atgaatacta agccaatcat
    45661 tatacatgca agtaaaggac tagaacaaga tacgcataaa cgaatctcag aagttttggc
    45721 tgaagaaatt ccagaagaaa aaagacaggc aattgttgta ttatctggtc ctagccatgc
    45781 tgaggaagtg gcagtacatg atatcacaac aattacagct gcaagtattg atcaaaaagc
    45841 agcagcctat gtacaaaaac tgttcatgaa tgattatttc aggatttata caaataatga
    45901 tgtgattggc gtagaaactg gtgcagcact gaaaaatatc attgcaattg gtgccggcgc
    45961 tatccatgga ttaggattcg gagatgatgc aaaagcagca atcatgactc gaggattggc
    46021 agaaatcagc cgtcttggtg tcgcgatggg tgccaatcct ctgacattta tcggtttgag
    46081 tggtgtgggg gacttgatcg ttacttgtac aagtgttcat tctcgcaatt ggcgtgccgg
    46141 taacttgtta ggacagggac ataagctgga agaagttcta gaaaacatgg gaatggtcgt
    46201 tgagggtgtg gcaacgacaa aagcagcagt agagctagct caacagctag gtgttgagat
    46261 gccaattact caaacaatct ataatgtttt gtataatgga gaagatatca aaaaagcagc
    46321 aaaagaaatc atgttacgtg acggaaaaat ggagaatgaa ttttctcttc gttaaataga
    46381 tgatcaatag ttaatcgt
//
LOCUS       DAFEOM010000016        41268 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.016, whole genome shotgun sequence.
ACCESSION   DAFEOM010000016
VERSION     DAFEOM010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.016"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            214..498
                     /locus_tag="LP524_001030"
     CDS             214..498
                     /locus_tag="LP524_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HBL2234099.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(779..1198)
                     /locus_tag="LP524_001031"
     CDS             complement(779..1198)
                     /locus_tag="LP524_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HBL2234100.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1200..1784)
                     /locus_tag="LP524_001032"
     CDS             complement(1200..1784)
                     /locus_tag="LP524_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBL2234101.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="LP524_001033"
     CDS             complement(1831..3306)
                     /locus_tag="LP524_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBL2234102.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="LP524_001034"
     CDS             3487..3798
                     /locus_tag="LP524_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234103.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="LP524_001035"
     CDS             complement(3913..6249)
                     /locus_tag="LP524_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL2234104.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="LP524_001036"
     CDS             6391..7059
                     /locus_tag="LP524_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2234105.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="LP524_001037"
     CDS             7062..8105
                     /locus_tag="LP524_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL2234106.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="LP524_001038"
     CDS             complement(8342..9223)
                     /locus_tag="LP524_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL2234107.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="LP524_001039"
     CDS             9382..10110
                     /locus_tag="LP524_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBL2234108.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="LP524_001040"
     CDS             10266..10583
                     /locus_tag="LP524_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL2234109.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="LP524_001041"
     CDS             10576..11184
                     /locus_tag="LP524_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBL2234110.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLVWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11239..12030
                     /locus_tag="LP524_001042"
     CDS             11239..12030
                     /locus_tag="LP524_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBL2234111.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            12168..12656
                     /locus_tag="LP524_001043"
     CDS             12168..12656
                     /locus_tag="LP524_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBL2234112.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12707..13579
                     /locus_tag="LP524_001044"
     CDS             12707..13579
                     /locus_tag="LP524_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBL2234113.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13755..14117
                     /locus_tag="LP524_001045"
     CDS             13755..14117
                     /locus_tag="LP524_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBL2234114.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14126..14878
                     /locus_tag="LP524_001046"
     CDS             14126..14878
                     /locus_tag="LP524_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2234115.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14892..16694
                     /locus_tag="LP524_001047"
     CDS             14892..16694
                     /locus_tag="LP524_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2234116.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16971..18011
                     /locus_tag="LP524_001048"
     CDS             16971..18011
                     /locus_tag="LP524_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBL2234117.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(18063..20207)
                     /locus_tag="LP524_001049"
     CDS             complement(18063..20207)
                     /locus_tag="LP524_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBL2234118.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(20564..21937)
                     /locus_tag="LP524_001050"
     CDS             complement(20564..21937)
                     /locus_tag="LP524_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL2234119.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="LP524_001051"
     CDS             complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="LP524_001051"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBL2234120.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="LP524_001052"
     CDS             complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="LP524_001052"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBL2234121.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23385..24398)
                     /locus_tag="LP524_001053"
     CDS             complement(23385..24398)
                     /locus_tag="LP524_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBL2234122.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24479..25078)
                     /locus_tag="LP524_001054"
     CDS             complement(24479..25078)
                     /locus_tag="LP524_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL2234123.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25264..25965
                     /locus_tag="LP524_001055"
     CDS             25264..25965
                     /locus_tag="LP524_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2234124.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25975..28686
                     /locus_tag="LP524_001056"
     CDS             25975..28686
                     /locus_tag="LP524_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2234125.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29137..30765
                     /gene="treC"
                     /locus_tag="LP524_001057"
     CDS             29137..30765
                     /gene="treC"
                     /locus_tag="LP524_001057"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBL2234126.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30825..32006)
                     /locus_tag="LP524_001058"
     CDS             complement(30825..32006)
                     /locus_tag="LP524_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL2234127.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32258..33781
                     /locus_tag="LP524_001059"
     CDS             32258..33781
                     /locus_tag="LP524_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBL2234128.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(33850..34578)
                     /locus_tag="LP524_001060"
     CDS             complement(33850..34578)
                     /locus_tag="LP524_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2234129.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(34591..35871)
                     /locus_tag="LP524_001061"
     CDS             complement(34591..35871)
                     /locus_tag="LP524_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBL2234130.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(36012..36614)
                     /locus_tag="LP524_001062"
     CDS             complement(36012..36614)
                     /locus_tag="LP524_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBL2234131.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            36859..37065
                     /locus_tag="LP524_001063"
     CDS             36859..37065
                     /locus_tag="LP524_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234132.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            37377..38096
                     /locus_tag="LP524_001064"
     CDS             37377..38096
                     /locus_tag="LP524_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBL2234133.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(38177..38860)
                     /locus_tag="LP524_001065"
     CDS             complement(38177..38860)
                     /locus_tag="LP524_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBL2234134.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            39078..41264
                     /locus_tag="LP524_001066"
     CDS             39078..41264
                     /locus_tag="LP524_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBL2234135.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNISVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        13385 a         7322 c         8276 g        12285 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccatat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcatg
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagttg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcattg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg attcgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atttgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gttttttttc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattgtac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaactt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 tttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tttgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgcta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa gattgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctggtttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgattagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg caatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aagcggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa aattaaaaaa
    12661 gtaacattag atatttattg aaggataaag gagcgcagga agtagcatga gagaaaaaag
    12721 agcattttat atcaatggtt atgcagggat tgtcggattg gtcgtattag ctttagttgg
    12781 attgtttttc ttctatttag gtatgtggca agcgaaagtt tgggcacttt ttttaagtat
    12841 ttttctttgg ttgatcacgt tattgttgtt aagttcggca actgtagtca gtcctaatca
    12901 agcaaaagta attttgtttt ttggacaata tttaggtacg attcgagaaa atggcttttt
    12961 ccttacgata cctttggcac aaaaaatgac tgtttcgttg aaagttcgga atttcaatag
    13021 ttctgtttta aaagtcaatg atttggatgg aaaccccatc gaaatctcag cagtagtagt
    13081 ctttaaagtc gttgatacag caaaggcatt attcgatgta gcgtattatc aagattttgt
    13141 agaaatccaa agtgagacag ccattcggca tatcgctagc caatacccgt atgatacatt
    13201 caatgagaat gatttgacgc ttcgaggaaa cacgacggca gtgtctgacg agttgcaaaa
    13261 agagctgcaa gagcgtttag cagtagcagg tgtcgaagta atcgaaacac gcttgaacca
    13321 tttagcctat gcaacagaaa tcgctagtgc catgctccaa cgtcaacaag caaaagccat
    13381 tttgtctgct cgacaaacca ttgttgaagg agctgtcaca atcactcaaa tggccttaga
    13441 gcaaatcgaa gatggacaag aaatcaattt caccgatgat cggaaagtcc aattgatcaa
    13501 taatttactt gtctcaatca ttacagacaa aggaacgcaa cctgttatca atacaggcga
    13561 catgaaagac cgtaagtgac atactttttg taagatagga agaaggagtt taggacggat
    13621 tattgtcttg aactcttttt ttagttgttt taaagaaaat gacaaaattg aaagaagaat
    13681 gatagttgat tcgaaggcag aattgttcgc ttttttctat aatcagtata taaagtaaga
    13741 gagaagggga aaaagtgaaa aaacttctga tcggtctact tgttttcatc gttgtggtac
    13801 ttgcaggggt gaaaatcgca gattacgtag ttatgggtgg agaagaatac tatgtacaaa
    13861 ttacgacaga tggtacaaaa acagtttcaa aatcagactc tggacaagaa acagtcaact
    13921 atagatatat cctgcctggc tatacgaaag agggtaagga aaaagaactg gacttcaagg
    13981 gacaattgac tcgaccgctt cgtaaaggag catatttaaa aatcacctgg aacaaaaata
    14041 aaggtgtcac ttcgtatgaa gaagtcgaaa aaaaagacgt gccgaaaaca gcactggaaa
    14101 aactaattaa agggtaggga aagaaatgaa caaaatagtc gaagtacaga atgtaacaaa
    14161 agtatacgga aaaaacaatg agaaaaagac gcaagcatta agcggcatca gcttcgatgt
    14221 agaaaaaggc gaatttattg ggatcatggg cgcttctggt tctggtaagt cgacactttt
    14281 gaatatttta tccacattag ataaaccaac agatgggcat atccgaatca atcaaaaaga
    14341 tgtgaccaca ttaaaaggaa atcaactagc agattttcgt gcgaatgaga ttggctttat
    14401 tttccaagat ttcaatttat tagaaagctt gacagcacaa gaaaatatcg ctgttcctct
    14461 ttctttacaa ggcgtcagac caaaagaaat caagcaacgt gtccaaaaaa ttgcagaacg
    14521 tttatccatc tctcatattt tggaaagcta tccttctgag atttccggtg gacaaaaaca
    14581 acgggtcgcc gccgcacgtg cattgatcac acaaccaacg attctattag gggatgagcc
    14641 tacaggtgcg ctggattcca aaagtgcccg ggatctttta gatacgatgg acgaattgaa
    14701 tacaaaagat catgtatcga ttcttcttgt gacgcatgat ccgttttctg caagttattg
    14761 ccaacgtatt ttattcatca aggatggcgg tatccatcaa gaagtgaaac gtggagatca
    14821 aagccgtgat tccttctatc gagaaatact atctattctt ggaaacttgg aacagtaggg
    14881 ggagattgac tatgctttgg aaattatcac ttactgggat caagagccgt ttaagagact
    14941 acttggtgtt attctcagga ttagttatgg catctgctat tttttacatg tttgaatcaa
    15001 tggcaagcaa cgaagctttt ttgaaaagta atacgatcgt tgcggctaca gttatcattt
    15061 tccgattcgg ttctgtttta ctagggatca tcacattcgt ttatatttta tatgcaaatt
    15121 cctttttgat gacgatgcgg caaaaagact atgcaatgtt tatgatgctg ggagcaaaag
    15181 gcagaaaaat cgcacagatg attttttctg aaacattctt agtagggatc gcagctactt
    15241 tagtgggttc agctgtcggt gtaggattga caagtatcgt ccaccgatta ttagtggatc
    15301 agttagatct caatatttcg cattttacac cttttagtat tcacgggttg ttgatcactg
    15361 ttttgttttt tgctgttttg tttttattag ctgctgttgt caatgctttt tcgattgtca
    15421 aaaaaccgat tttagcattg attcgtgcgg atcagacacc aacaagaatc aagcagcata
    15481 aattcttgtt tttccttgaa gtagtactgg gaattgcttt tctaggctgc ggttactata
    15541 tgatgaaaca cgtggggacc tatcaagtct ttggtatagg ggtagcctta gttacgatcg
    15601 tattaggaac ttatttcatt ttccattctg tgatcatttt ctttttatca ttactaaaga
    15661 aatcagaaca gatttcattg aaaaaattga acaatttcac attgtcacag ttaaactttc
    15721 gtattcgtga atatacacaa atgctctcaa tggtcgctat gctgtttgcc ttggcactgg
    15781 gcgctttgac ggtaggatta ggttttaaaa atgaaattgc agacttgaca gaaaacaatt
    15841 ctggatatga tctagtgatg aataatcctc gggcagaaga tcagcaaaaa atcaaagaac
    15901 tttcaccaac actttctgct tcttatactc aaaaagaaga tgcgggaacg atttactata
    15961 atgcaagtga atttgcggct caaccattga tcatgataaa acatgaagat ggaacaccgc
    16021 cgaagatcac gaaagaaaaa gttcctgcta aaaaaatgaa tgaactgact gtacaagagg
    16081 aacttcgaag ctatgaattg ccggaacagc aagaaaaaga aatcaaggta gtcagtcaat
    16141 cagaatttga tcaactgaac ttgccacaat ccactttaca gctagttcag gtaaaagact
    16201 tccaagcaaa cctgaaacca atcgaaacac ttgttgctca aaataaaatg aacaatccat
    16261 ctttgcaaaa agtagagtac agcaaccaga aatatgaaat gtatgaagtg tataatggtc
    16321 tattctctgg ttttgaattt atgggattct tcctaggttt agctttcttg accatgcttg
    16381 ctagctgttt gatgttcaag atcttgtcag gaagcaagag tgacattgct cgttatgaaa
    16441 tgcttgaaaa gataggaaca cgacgcgggt tgcttaaaca atcgatccgt agagaagtag
    16501 gcgtgttatt cttagcacca ggaatcttag gtgtagtcca tgtcttattc ggattacaaa
    16561 tgtttggtct attcatgcaa aatccataca aagatatttg ggttccgttt acgatattct
    16621 tcgtattgta tttcatctac tatgtactga cgacatggtt atatactgga atcgtattga
    16681 aaaagagatt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa gcaaaaatag
    16741 ctcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    16801 ttgttcggaa aaagaggttg tgacaatatt tttgtcacaa cctttttttc acggtctttt
    16861 attagaatat agtaaatttt ttctcgttac tcacggaata agctgttaag aaaacggttt
    16921 ccatgtatgc tggaatcagt gcaaatgaaa gaaaagaggg tgcaaggaac atggaaatca
    16981 atgaacaaaa ttttggcgaa aactatcact tgattacgat cacgaatcaa aatgggacat
    17041 ctttgtctgt cagtgatctt ggggcacggg ttgttcattt tcaaacaata atcgataatg
    17101 aaaaacgaga gttgatctta ggttttgatg aagcaaaaga atatctagaa aaagaccctt
    17161 atatcggtgc ttcaatcgga cgtatggctg gacggattac gaatggcaca ttcaaattag
    17221 atgatgaaac gttcgaagta aaaacagacc cggacactgg tcattgttta catggtggtg
    17281 cacctagctt tgaagccaag cagtggcatt atacagtcat caatggtgaa aacgaagcat
    17341 cggtcatttt ctatcagact agcccagata tggaaaacgg atttcctgga actttagatg
    17401 tagaagtacg gtatacgctg acgaacgatg atacatggcg agtgacggtc caagggatca
    17461 gtgaccgcaa aacagtattc aatcctacaa atcatgtcta tttcaattta accggtgatc
    17521 cagcacagtc aatcgacgaa catatacttt ggttgaatag ccgattattt gctacgctca
    17581 ataaagatac aacgcctaca ggagaagtag tttcagtaag tggcacagca tttgacttcc
    17641 aacgtcctaa aaaattaggt gaggtctttg ccagtgattt tcgccaatca atgatagtgg
    17701 gcggcatcga tcatccattt ttgttgaaaa aacaatctgg tttactaaat gctgcggaat
    17761 tgacaagccc agatgaaaaa gtatcgatcc aagtaaaaac agatgcttca ggaatcgtga
    17821 tttttacagc aaattttgga gaagatggac cggaaatgcg cgggaataaa ttagtaaatc
    17881 atggcggtat cacatttgag actcaagaac tgccaggagc ggagcgactt gaaagttttg
    17941 gagatgtcac tctggaacct aaccaagtac gcgagtttgt aacggaatac aaaataaata
    18001 taaagaaata gaataaatta aaaagggagt gtgacagtgt cacatcccct ttttaattta
    18061 ttctatttct ttaaaggcag atcaagccag aatgatttgt ctaaacttaa caaggttagt
    18121 acaggaacag ttgcagcttt aactgtacga gtagttgttt ctggtatctg tcttgccgta
    18181 aaattgttcc acaaatccca gattgaagcg acacggaaga tggaagaagc gttgttgacg
    18241 cgttttatcg ttgaaaattc taatcctgtg attggcacgc catctgttgt ccatttgatt
    18301 ggcacatcga atcgcttaga catattgagg atttcagcgt ttgcttcttc agttgttgct
    18361 gcttcttttt tcggttcatc atattttgtc aggtcgtacg cttcgatgat cgcgttcaat
    18421 ttttgtgaat attggcgatc tgtagcataa cgtccttcca aatatttggt tgcttctttg
    18481 tagtcttttg tttccgactt ccaagttggt ttgtagaaat caggattgcc agtcagtcca
    18541 tttttgatca agtcggcata gtcttccaaa ctttccttgt aagaaggata tttacggaat
    18601 tgtgcgcgga tttggtagct ttgtccggaa ctatcttgtt caagtgtatt gaaggtcacg
    18661 ctgcttcctt tatatgatcc tttgatacca aaaaggttat gatttggttc gcttgataat
    18721 aagctgtttc cagatccact ttcaaggata gcttgagcca tcatgactga tgcgtagatt
    18781 ccttcttttt gcccgatttc ctgtgcatca tctgcaatct ttttcaagaa ctgatcagtt
    18841 gtttggtttt gactgaaatg gaacgagcca tctaatccat ctaaaataga tgagccgcct
    18901 gtcagcgaac ctgaagcagc aggtaaacct gctaagttta ctcggacagc aaaatcaggc
    18961 gtgaaatctg aaatagaagt tactcgaact ttatctcctg gttgaggcgc atggataaat
    19021 tgtccgttcc caatatagat acctacatgg tgagtaacac cggaagaagg tccccagaag
    19081 acgaggtcgc caggttgggc ctgacttact ggaatctgcg tacctgcata ttgctgttcg
    19141 cctgtccagc caccgatata atgaccggtt gctttcatat atacgtaata agtcaaacca
    19201 gaacagtcaa aagtatttgg accttttgct ccccatacat atggttttcc aagatgcttg
    19261 aatgcttctc gaacgattgc ttgttggaca ttcgttgtat ttgtaggcaa tacagcatca
    19321 attggtgatt ttacaacaaa atcagaagaa gcagaagatg gattctctat accgctttga
    19381 tttgctgtat ttggacgaga tgaagcattg ttcgttccgc cattcgatga accattgttt
    19441 ggatggtttg gttctctctg agtatttcca ttgttattcg atccagatgg gtttgtttga
    19501 ttcgaagaat tcccgctgtt cgaattattg ttattattgt ttgaattatt tgaaccatta
    19561 ttattgttgt tattacccac atctggtttg tttggcgttt ccggatctgg tgtactaggg
    19621 acttcttcac taggatcagt tggtttctct gttgaagtat ccggtccagc tttaaattct
    19681 gcagtaatag ataaattgtt cgtacctact gtaaaggtct ttgcatcata atttacttct
    19741 ttggcaccat taacagcgac tgaagcaatt tgttcacctt cagctggcgt aatagtgtaa
    19801 gttaccttcg tttccgctaa taaaccttct accgttccac tttccgtaat atccaaaggt
    19861 tgctgttcag gggaatccgc tgtcgaagca gtcagttgga tcttaccaat caaagaagga
    19921 gcaattgtaa gggaatatgt ctcggctggc ttttcatttt ctgatggatc agccggatca
    19981 atttcagttg gctcggtcga tcctgttgtt gagttagttg ttccagttgg gtcttctgta
    20041 aaagaatccg ttgtttcact agtagctgag ctggatgtag tcgtatctgt tgtgactgtt
    20101 tgatcagcta tttgattatc tacggcaaat acggtattgg tcgacaaaac agacggagca
    20161 atcagccaag tagcagctag caaacgtatc gtttttttat ttttcataat aaactagtcc
    20221 ttttcctttc attgattttc tgtcttattc agcattcatt agacattttt tggaacagat
    20281 aatcgtgtcg aaaagctttt tcttttcata aatctgtctg tgtattctaa ataaaacaat
    20341 ttgtaaataa gcaacgagca ttcattcgtt tttctttgac gttcatttta ttgcatagac
    20401 cacctttgtt tggggataaa caagtgggga taaaaatggt tatgcatatg taaattatac
    20461 caaagtttgg aaagtatcac aaaaatttat aaaaataatg atcctaaaaa gaaaaagact
    20521 gaaatatgtg ataaacatct catacttcag tctttttcat taactattca taagacatta
    20581 ggtcttcatc aaacccgcta acgagagtag tgatcattgc tacgactaga ctaacagcca
    20641 gtcctgctaa gtaccagaaa aaagtaccgg aaatgaatgc aggcaatgtg gtcagactac
    20701 caaatacaaa agcgatcgct ttcacttggg caaatcccat tacagctcca ccagcagctg
    20761 caccaatgca agcagcaaca aatggttttt tcaatgggat cgcaattccg taaacagttg
    20821 gttcggtcac tcccgcaagt aaaatcgaag caaaagcaga accggagatc gaacgggttt
    20881 ttgaacgttt acttcgcaga aatacaccta aagctgcacc agccattcca aaattggctg
    20941 ctcctgtcaa cggaacgatt cggtcgaacc cataagcaga gatattactg atcatgacag
    21001 ggtttacagc ccattggata ccaaatatta ccaaaatatt ccaaccacca gcgatgattg
    21061 cgccactgat cggtccgtta atatccatca tccaagtaat tgcggcagct aaaccttcgc
    21121 caaggtaaac acccgcaggc ccgaatacca ttaacgtcaa aggaaccgtg ataactaatg
    21181 aaagtaaagg tacaaatact aattgcatat tttcgcgaat atattttttg aggaattttt
    21241 caacaaaaga ataaaagaaa atacctaaaa tcgccggcat gacagtagac gtatactgca
    21301 tcagtactac aggaatcccc agaaaatcag tagctgcttt gccactgtct gaaagtaaag
    21361 aggtcagatt cggttcaatc agtgctgcag caatgacagc cgcaatatat ccattcactt
    21421 taaacgtttt ggcagcagaa aaagcaagaa agattggcag aaaataaaag acggcattcg
    21481 acgcagcaga caaaatatga taagttccgc ttgtttcact aagccagcca atcgtagtcg
    21541 ctaaaacaac cagacctttt aatataccag aaccggcaaa cagcggaatg atcggcgtaa
    21601 agatttccga tatcaaacca aatagttttt cgatcggatt tagcttttct ttcggagctg
    21661 aatgatcgat ttgacgcgtc aattccttgt aagcaggagc caatccctga tccatgatta
    21721 tttctgcata tctatcgagt aaaatgaaat acttaatgga agggatttgc tgttttaatt
    21781 tttctgcatt ggctactggc tgttttttca atttgatacg aagccgagtc gcacatggaa
    21841 ggacagaatc aatattgtct tttcctccca actgttcaat cagcatatga atctgctcgg
    21901 acagtaccat ctgttcagga ttcattgtcg tttccattag aagaccactc cactcactaa
    21961 tataatattg gcgtaaggtg tcatttcgcc tgtacgaata aatgcatgac agttattcaa
    22021 ctcttttttc atttcttcat gtgggataaa gctaatcggt gtatctggta acaataattt
    22081 gattttttcc aatatttccg ggttcattgt ctttatttct tccgccaatt caatcgattg
    22141 tacctcaagt tcttctaaaa cgttttctaa aacagataaa aaacttggaa gaccgtttga
    22201 gacagccaaa tctatttttt cagtaccaaa ggggacaggc atgcctgcat ccccgatact
    22261 taatttatca aagtgcccca tttgtgcgat tactcgtgaa atatctgaat tgattacttt
    22321 tccttttttc atgtttgttt cttcctctca ttgttaattt tagactgctg attgttctaa
    22381 agcttcgcgg taagggatag aaggttgcgc accataagac tgcacagcaa cagaagaggc
    22441 tagatttcca taaaggatcg cttctttcaa gttactaagg tctggctgaa gtgtgctgct
    22501 taatgcgcca ataaatgtat cacctgcggc tgttgtgtca acggcatcca ctttaaaagc
    22561 agggacgata ccgtgttctt tttctgtatg atagaaagcg ccggcactgc ctagtgtaat
    22621 gattactgtt ccaataccta attcatggag tttttcggct gctgcgacca aactgttatg
    22681 atctgtcact ctgattcctg taatgatttc tgtttctgtt tcatttggta taatgatatc
    22741 tgtcttttca agtagttcag taggaatcgt ttcacgagct ggtgcaggat tcaggatcgt
    22801 tgtttttcct gcgtcttttg caatagaaaa agctaggatc gtcgcatcta agctactttc
    22861 aaactgagca ataataaagt cactgtttgt aatcagttgc ttgtttttta ggacttcttg
    22921 ttcatggaaa gcattatttg cccctgcatg gatcaagata ctattttctc ctgctgcgtc
    22981 taccataatc attgcttgtc cggttgtggc gccttggatt tcagctatcc cagatgtatc
    23041 gatgttttct tttgtcagta gatctaataa ctgttgtcct tcgctgtcag ccccaacgcc
    23101 tccaataaaa gatgtccgtg cgcctgaacg tttggctgcc actgcttggt tggcaccttt
    23161 acctccgcca gcgtgaaaaa tctcatgcgc atgcatcgtt tcgcctggct tgggcatgtt
    23221 ggttaaacga agggtagtgt ccatattaat acttccaatt actgtaaccg tgttcatttt
    23281 acaaacctcc aactatctca tttattttga tgggaaaatt attttttaaa acgttttagt
    23341 aaaacgtttt aatagttcga taaaaatata gcacaccttg aaagctattt caagggtgca
    23401 gttgaaaatc ttttttctaa tcgtacaggt aatctttttt cttcccattc tttttctggg
    23461 aattggatcc gatctaacaa taacttggca gatgtttgtc ccaattcgaa gattggttgt
    23521 gcgatcgttg tcagtttcgg cttgatatac tcgcacatat ctatattatc atagccaata
    23581 atgctgtagt cttcagggat gctttttcca gcttcctcta atccgcgata taatccaaaa
    23641 gctaattcat catttaaagc aaacacacca gttgcagcag aatcaagaag ttctgctgtg
    23701 atctggtagc caccttgttt tgagaactgt gtaggtaaga ggatcagttg gtcgtgaggg
    23761 atttggtaaa catcaagagc agatttaaac ccctcaatcc gagcgtggac attttccggt
    23821 ggattttcag gatagacaag ggcgatcgtt tgatgtccta gactaagcaa gtgcatgccc
    23881 gctaggtaac caccgcgaaa atcgtctgtt cgtacggcat cactaaatcc ttcagatttt
    23941 ttctgatcca aaacaatgaa gggccggcct tgttttttca aattttcgtt gattgcatct
    24001 gtagagacag cagaagtcgc gataatgaat ccatccacac ctcgacgtgt gagctcggct
    24061 agatattcga tttctttttg atgatcagaa tcagcattac ataatatcgt gacgaaattc
    24121 tgcttataca agatatcctc aattcctcgc atcagtgtac tgaagaatgg attcgtgata
    24181 tcaggaacga gtacaccaat tgttttcgtt tccttggtaa tcatttgtct agcaaaatag
    24241 tcaggctgat agcctaattc ttctttggca gctaaaactt tctgaacagt tttcggactg
    24301 aatttctccg tatttccatt taatatcaag gagacagttg tgattgaaac gccggaaagt
    24361 ttagcgactt ctttgattgt gatctttttt ttcaccatgt tcatactcct atctaaatcc
    24421 tgtggttatt ttcccatgaa atcaaaaaga agtaaatgaa aatcacgtgt gccagtactt
    24481 atttgctttg ttggatagct tcgattattt gtttcgttga gcgtactcgt gcagttttcg
    24541 gaaatatctt ttttatgaac aattgatggg attctttgtc acgatcactg caagcatctt
    24601 cagcaagcac gatatggtac ccgtgttgaa aggcatctaa tgcagtagca tatacaccgt
    24661 ttgttgtcgc tacaccgctt aaaatgatag tttcgattcc acgccggcga agttgaagat
    24721 caagagaagt accaaaaaaa gcacttggat tgtattttgt gatagtcaac atattatctg
    24781 tatctttcaa agaatccttc aaaagtaaat cagtgaattc tggaggtaca gaagcagtca
    24841 ttccacccat atcagtttgt tgactcaaat aagggaaagt ggagatgtct acgttcacta
    24901 aaactttcaa ggcagcggta cgtttcaatg ttcttactaa caaatcattg tttgtcagaa
    24961 tagatgctgc agtgtgtgga gagagtgtac cgttatttac gatctctttt tgcatgtcga
    25021 tcgcgacaaa agcagttttt gataaatcaa aaatcatatt ttcctttaaa tcattcatga
    25081 tgttccttct ttcaaataac aatgtttttt atgtaagtaa atcataaatc gtttatttgt
    25141 caactaaatt aaaggattcc tttctttagt ctagagaaat tcagtggtaa aataaataag
    25201 tgtgagaaaa cgcattcgat gcatagatat agcgagaaat atcgtgaaaa tggaggaatc
    25261 agagtgaagt atgtcgaagt aaaaaatgaa tacaaacgat acaaaatggg agaaacgacc
    25321 attacagcaa atgacgatat ttctttttca attgaaaaag gcgaattagt gattatactt
    25381 ggtccaagtg gagcaggaaa gtcaacgatt cttaatattt taggtgggat ggatacacct
    25441 gatgaaggtc agatcattat tgatgacaca gatattgcgc aattttcaga taaacagctt
    25501 actgcttacc gacgaacaga tgtagggttc gttttccaat tctacaatct cgttcctaat
    25561 ctaacagcaa aagaaaatgt ggaattggca acggaagttt ctcctgatgc gcttgatcct
    25621 gtagaggtat tgaggcaagt gggcctagct catcgattga ataactttcc gtcccaatta
    25681 tcaggaggcg aacagcagag ggtttcaatt gctcgtgcac tagctaaaaa tcccaaactt
    25741 cttttatgtg atgaaccgac aggcgcgctg gattttgaaa cgggcaagca agtattgaaa
    25801 ttattgcaaa acgctagcag acaacacggc aataccgttt tgatcattac acataattct
    25861 gctttagcgc ccatcgctga tagagtcatt cacattaatg atgctaaagt aaggtctgtt
    25921 gaattaaatg atcatccttc atccattgat gagattgtat ggtaggggga ttttatgaaa
    25981 acaagaacat atcttaaagc aggttttcgc gaaattcggc aatctaaagg acgattcatt
    26041 gcaatcatcc ttattgtcat gctaggaaca cttttatttg taggtgtcaa aactgctgga
    26101 cctgtcatgc aaaaaacgat ggatcactat gttggtacag caggactttc tgatttgcaa
    26161 atcgtttcta ctggtggctt gacacaaaaa gatattgctc aagcagaaaa gatcccagat
    26221 gcaaaagtag aaacaggaaa acagatctat tattccaata ctggcaaaaa cgaagtgatt
    26281 caaatatttt cttataacaa aaataacaag cagaacaagc tacaattaac tgatggaaaa
    26341 ctcccggaaa aaccaaacca gcttgtattg gatgaaaaag cggaagatga agggtataaa
    26401 atcggtgata tttatcagat tgattcagat gaattgaaag aaaaagagta taccatcacg
    26461 ggctttgtcc gttctccatt gttcatcaac aatttggaga gaggatacgc gaacgtcgga
    26521 aatggtagcg tcgattattt tgtctattta cctgaatccg tttttaaaac agatatttat
    26581 tctgtcttat atcttgattt tacaaacgta aagaatttga atacgtacag caaagcttac
    26641 aaagataaaa tggagcaaaa tcaagaaaag gcagaaaagt atttacaaga tcgacccgaa
    26701 gaacgcttag aagagctgaa aaaatcggcc aacgaagaat tagaaccggc acagcaaaaa
    26761 atagcggatg gcaaagcaca aacaacgcaa gcaagaacac ggttagaaga tgctaaaaaa
    26821 cagttagcgc aacagtcagc aactatccag cagcttcctg aagcgcaacg aacaatcgct
    26881 gttcaaacat tatctcaaca agaagctcaa cttgctgaac aagaaaggca attagttgag
    26941 aaagaacgtg aactggcctc tgcagaggaa gaattgaatc gaaatgcaga aaaaattgaa
    27001 aatttaacta aaccatcata cctatacaat gaacgttcag aaaatgtcgg ttttcaagaa
    27061 tttggtgatc tgtctgatcg tatcgctgcg attgccaatg tttttcctgt tttctttttc
    27121 tttattgccg cattgatcac ctttacgaca atgacacgta tggtagaaga aaaccgcagg
    27181 gaaatcggga cattaaaggc gttaggttat acgaaagttg aaatcgcaaa aaaatatgca
    27241 atctatgcaa gtctagcctc agggataggt attgttttag gaacgatatt aggaacgaat
    27301 cttttgccaa ggatcatttt tgagctgtct aacgagcggt atgatattgg gagtgcgctt
    27361 attttttatg actggccgcc gattttacaa gcagctgccg cattcttcat tgccgcattt
    27421 ggcgctgcaa tgatcgtttt attcaaagat ttaagagaac ggcctgctgc attgcttcaa
    27481 ccgaaagcac cgaagccagg gaaacgcatt tttcttgaat acatcactcc gctgtggtca
    27541 cgccttagct ttaatcaaaa aatcagttac cgaaatctct tccgatataa gtcaagaatg
    27601 cttatggcga ttatcggaat tgctggatgt gccgggctga tggttgcagg cgtgggattg
    27661 aaagattctc taagttctgt ttcagccaaa caatttggaa cgatcattga ctatcaagca
    27721 atcgttacat tggattcatc cacggaagat agtcgtcaaa aagcagcgga tatgctgcag
    27781 aagcaagaaa aagtaaatga gatcctagct attcatactc aaaccgtaga attgagaaaa
    27841 aaaggacatg cttcgcaaag tctgacagtg atcgtaccaa gtaaaacagc tgatttggat
    27901 cgttttatcc atttaactga tcttgaagag aacccacgct ccttgcctaa aacaggaatc
    27961 gctattacgc aaaaagtagc agaactattt gacttatcta caggagatca tatttcgctt
    28021 tatgatgaag atcaaaaaga atggaaagtc gaagtaaaag aaatcgtgaa aaactatcta
    28081 ggtagcttta tttatatgtc ccctgaatat tatgcttcag ttactgatca atcgatgatg
    28141 gataatgcat ttttgctgcg tacagataag atgacaacaa aagaagaaga aatgctttcc
    28201 gagaaactgc ttgccacaga cgctgtgaca aatacgtctt tcatgtccaa acaaatcgaa
    28261 acgcaagaag aatcaatggc gaacttagac tcggttgtct taatctttgt cgtattatca
    28321 ggactgctgg cttttatcgt cttgtataat ctaactaata tcaatatttc cgagcgtgta
    28381 cgcgaattat ctacaataaa agtccttgga ttttttgata aagaagtcac gatgtacatt
    28441 gttcgagaaa atatcatctt tactctttta ggaatcatcg gaggatttgg tgtaggatat
    28501 atattgactg acttcatctt gcaacaagcg tctatggaga atgtcatctt tccattagtg
    28561 atcacatggt gggcttatgt cttatcagca ggattaacta tcgtatttac ggttattgtg
    28621 atgattgtta cccatttcaa attaaagcat atcgatatga ttgatgcgtt gaaatcgaat
    28681 gaataaaaac agaggttgtg aaatcagcgg tttgacctga gtattagaga aacaaacggc
    28741 aaaatagttt ttcatatttt gccgtttttt gagctaatac cgaaggacag ctgattgaac
    28801 accatgaatc agaggttgta aaaaagccgt cctgcatcga gcaaaaagaa tactcaaata
    28861 aaattagctc ctcatgtttt acatttgaga agactgattg ccgtagataa aagcttttga
    28921 acaccattta tagaagaaac aagaatattt tgctatttta aacaagagga tagacaaata
    28981 catgtctgat ctcttgtttt ttattgggca acagacctct tagaatgtat aaagataaat
    29041 tttacaaacg ctttcggttc agttataata ataaatgata acttgtatat ataagttgtg
    29101 gtaggtaata aatcagttga atggaggtaa atatccatga catttaaaga caaagtcatt
    29161 taccaaatct atcccaaatc ttacaaagac agtaatggtg atggaattgg agatttaaaa
    29221 ggaatcaaag aaaaactccc gtatctgaat gatctaggca tcgacatgat ttggctaaac
    29281 cctatttatc caagccctca aaaagacaac ggttatgata tctctgatta cacagccatc
    29341 gatcctatct ttggtacgat ggaggaattc gaacagctaa tagccgaagc aaagaaatac
    29401 cggatagaaa tcatgctcga tatggtgctg aatcacgttt cgacagaaca tgaatggttc
    29461 aaaaaagcac tagcagggga ccagcattat caggacttct tcattttaag agaagagcca
    29521 acggattggg tatcaaaatt tggtggaagt gcttggtcgc catttggaga tacggggaaa
    29581 tattatttgc acttatatga tcggacacaa gctgacttga actggcggaa ccaagcagta
    29641 agagaggaac tattcaaagt tgttcgtttt tggatggaaa aaggggtaaa aggattccgt
    29701 ttcgatgtta tcaatgttat cggtaaagac gaacaattga aaaataataa agaaaatgat
    29761 ggcaaacctg aatatacgga caaaccaatc acccatacgt atctaaaaga attgaaccaa
    29821 gcaacgtttg ggacggattc cactatcatc acagtcggtg aaatgagctc tacgacgatc
    29881 gaaaattgta tcttgtatac agaacctagt agagaagaat tatcaatggt ctttaatttc
    29941 catcatttga aagtcgatta tgaaaatggg aataaatggt ctactcctcc atttgatttt
    30001 gcagaattaa agcgactatt tcatacatgg ggagaaaaga tgagtgaggg taacggctgg
    30061 aatgctctat tttttaataa tcatgatcag ccacgtgcgc tcaatcgatt tgtagctatc
    30121 gatcagtatc gaaaacaagg agcagagatg ctagcaacca tgatccattt gaaccgtggg
    30181 acgccctata tttatatggg agaagaaatc ggcatgatcg acccagacta ttcatcgatt
    30241 tccgactata ttgacgtaga aagtttgaat gcttataagt tactccttga acaaggattc
    30301 acagaaaaag aagcgtttcg tcggatcaaa gcaaaatctc gcgataactc tcgaacaccc
    30361 atgcaatggt cagacacaga acaagcagga tttactacag ggaaaccttg gttaaaagtt
    30421 ggaggcaagc tagaagagat taatgtagaa aaagaactat cttctgaaga ttctattttg
    30481 tcctattaca agaaattgat tcggctgaga aaaacatatc caatcgttgc acaaggagat
    30541 tatcatgctt atgaagcaaa tcatccggaa gtttatggct ttcttcgcca atttgaagga
    30601 cagcagctgc ttgtattaac aaatttctat gataaagata caaggatcgc catcccagaa
    30661 ggattcacag aagccaatat tcttatcgat aattattccg atactgttct tcatcaaagt
    30721 ctggagctaa aaccatacca ggcaattgct ttgctgaaaa attaaaacaa aaacacaaaa
    30781 cgtcgttcct acgagctatt ctcgtaagat cgacgttttg tgttttattt aaaataatct
    30841 tcttcagaaa atgaagagtt ttcatactct cctttatcgc cataaaattt attgtagcgt
    30901 tgtttgaatt tacggaaaat aaaagtaacg agtaatttga ctaacgtata gataggaata
    30961 ccaaaaataa cgcccacaat tcccattaga tcacccatga caagtaaaac gattaagatc
    31021 gtaatcggat ggatctgtaa acgatcaccc attactcgag gaatcaccaa gtcaccgtgg
    31081 agaagttgta cggcaaacca gacaatgaca aatttgacag ccataaaagt agagtcttgg
    31141 aatgcaatga tcaaacctgg aaagaaggcg ataaacggcc caatatatgg aatgatacat
    31201 aacaacccag cagaaatagc taaaacacta gcaaattcta atccgatcag taagaaacaa
    31261 acccagtaga ctgctccaag gataaatgaa gcaatgattt gcccctttag aaatgaaccc
    31321 aattgttgat tagcaatctt tgtcaaattt ttaaaatctg tacgaaatgc tggtgggaca
    31381 atcgctaaaa ctgactgata aaatttttga ggatctctca aaaggaaaaa agcgacaatt
    31441 ggaccagtaa ataaagtaat aaaaatagct gtaacagttg aaaaaatgtt gctgatccgt
    31501 tgtgcgccac tttcccaata atttccaata aatccagcta tatcagacgt cacgtcgtca
    31561 atagaagcga aaaattgatt aaatgaatcg gcaaaaggtg ttttctccaa aaattgtcgg
    31621 agactctctt gaaaatcttg aaacaaatct ggcaaactac tgatcaatgt ctttgcctgt
    31681 tcttcaagaa aaggaataag ccatatgctt ccaagcccca ataaaaataa aataatcaca
    31741 taaacgataa tgactgacaa gttcctcgaa aggtgcttgc tcaaacgttt gatgactggt
    31801 tccaaaagat aatacaagat cagtgcaaac aataatgggg gcaagatagt agcgataatg
    31861 attccgacag gcttgaatat aaacgagatc tgatggaaaa aataaatgat cagcgcaagt
    31921 aagataacta ttcctaaaac atagtaagag gtttttcctc ctaaaaagcg gatcaaacga
    31981 ttcgttgtat ctgtttcttt tttcactctt tttcctcctt tatctataac ttcattatag
    32041 acaaaagcgg gtataaaagg aaaatgatag aggttgattg caaaaatatc gtcaatcatt
    32101 catataaact ttctcatcaa ttaaaataag aaaacgcttg acagttcttt taaccatatg
    32161 ataaattaaa tcctgttgct aaaacgtaac tattacggtt tgcgaaaagc gaatcggaac
    32221 attcaaatga aagaagatga gaagaggagt gtcataaatg aaacgattga taggtatatt
    32281 ggccatgctg gttttagcag gtatgttaac tgcttgcggg gcaagtggga aagccgagga
    32341 cagtaaagaa aaattgagcg tgatgacgac cttttatccc atgtatgatt ttacaaaagc
    32401 aatcgtcggg gatgaaggag aagtagagtt attgattcca gcagggactg attcccacga
    32461 ttatgaacct tctgctaaag acatggcaaa gatccaagat acagatatct ttgtttataa
    32521 tgatgaaaac atggaaacat gggtccccgc aatccaaaaa acattacaag aaggcaatgt
    32581 ccatacgata aaagcgacag aaggtatgct tttacttccg ggaagtgaag aaggacatga
    32641 tcatgaccat gaacacgggg aagaaggcca tacacatgag ctagacccac atgtatggct
    32701 agctccaagt ttagcaatca agcaggtagc aaacatacgt gatcagctga ttgaagctta
    32761 cccagaaaaa caggaagtat ggacaaaaaa tgcagcagcc tataccgaga aacttcaagc
    32821 actccatcag ctttatcaag aaacctttaa gcaagcaaaa caaagatctt ttgtcacgca
    32881 acatgctgca ttcaactatc tagcgttaga atatggattg aatcaagtat cgattgctgg
    32941 tctttcttct agtgaagaac cgtctgcggc gagaattgct gaactgaaac actttgttaa
    33001 agaacatgga atcaactata tttattttga agaaaacgcc aaagatagta ttgccagaac
    33061 gttagcaaac gaagcaggtg tatctttaga agtattgaat cctttggaag gattgacgaa
    33121 tgaacaaata gaaaatggag aaaactatct ttctatcatg gaagcaaacc tcgaagcgtt
    33181 gaaaaaaacg acagagacag aaaacccgct agaagatact ttgaccccca aaaaagaaaa
    33241 gagcgtctac aacggttatt ttgaagacga ggatgtacaa gatcgctctc tttcagattg
    33301 ggcaggaact tggcaaacag tagatacgta tgtagaagat gggacttttg atcctgtttt
    33361 tgaatataaa gaaaaattga atcaggataa gacagcacaa gaatataaag aatattatac
    33421 gaagggctat caaacagata ttgcttcaat caagatcagt gagaaagaga tggcatttac
    33481 gtttaagaat ggaacaacga aacgttcaaa ttatcgttat gtagggaaag aaatcttgac
    33541 ctacgaggca ggaaaccgtg gcgtaaggta tctattcgaa gcagaagata aggaaagtgg
    33601 cgcttttaga tatattcaat tcagtgatca cacgattgca ccggctgact ccgaacattt
    33661 tcatttatat gcaggaaatg aaagtcaaga agtattgctg aaagaaatgg agaattggcc
    33721 aacattttat ccagatacaa tggatggacg tgaaattgca caggaaatgc ttttgcactg
    33781 ataggcaaaa aagaaccgaa gggtttggat cggctggtca tttagtcggt ccaaactatt
    33841 cggttctttt tatgaaatca atttctttaa tccacggata gaggctggga tggtctcgcc
    33901 gtttgccaac gtgacttctc tggttttttt attaatcgac cgcacatttt gtgtattgat
    33961 aacataggat ttatgacaac ggataaaatt ccgatgcatt ttctcgattt ctttgatctt
    34021 ctcatagaat tgtaattggg catgagtcaa atggacttcc agtttatgtg gcgctcctag
    34081 cgaagtagaa aaatattgga tgtctttgat cggtaaaaat ttgatctcat cattaaactc
    34141 tattttgaag atatcttttt ggatttggga catttgaaaa tcttcgaaaa ttttgctcag
    34201 gatatgctcg acattttctt ttaaatcata aagatcttct ttgacgatat aatcagtcgg
    34261 ttcgatgttg ctttttaaga ttttcatcga catttccgta tgggaagtca catagattat
    34321 ttttcctaaa ggatctaatt cacggatcaa tttaccgatc gctacaccat ccatttcact
    34381 gtcttctagt tcgatatcta aaaagtaaat accaaaagaa gaagttctga tcttggctgc
    34441 attgagaagt tcttccggat cagaggtaga aatatggaca taactgtcta agttttcaat
    34501 catgattcta tttttgatca ccgttgataa aacttctaat tgttttggat cgtcttcaca
    34561 aatataaatg ggaatcatat cattactcct ttattttatt tgtatataaa tcgtttgaga
    34621 aaaacgctgg ttttcaattg atgtttcaag gaaaagataa ggattggtct ttgtcaattc
    34681 atccaagata aacaagccaa ggccttcgtt ttttggattt gtttttgttg agaacccttt
    34741 ttgtttcaac tggtttaacg gtaatgtatt tgttgttgta ttgatcacac gaatcatcac
    34801 ggaatgattc ttcttgagta tggtcaattc gatttttttc ttctttgcag cactagctcc
    34861 ttcaatcgca ttgtccaaga taataccaag catacgtaca aggtttaccg tatgtttcgt
    34921 atccagttcg attggctcag gaaaatgaag ctggacgatc aacccttttt cctgtgccat
    34981 catgagtttt aatgacagta aactacggat ctcaggaact ttcatattat ccaacatact
    35041 tagattggcc ggaaaaaggt tcatcatctg gcgtgtaggt aatatcgttt gatcataata
    35101 agaccgtaat ccttctaagt cgccttctga aatatagctg tgaagagaaa aaagcagatt
    35161 ttggtaatca tgtttgaatt ctctcaagtc ttgatagttt ttttctagat ctttgatata
    35221 atcctgttgc atcctgactt gttgctctaa taactgatca gctaatttct tattagagat
    35281 tttccaataa taaaccaaga tgatcaggcc aacacacaga tatagaatga aaaaactcgt
    35341 attcaaagct aagacatcag gtgtttcacc agaaaaacgg gtataaaaaa taatgatata
    35401 gtatgtaaat aatatcaatg tcccgatcgt accgatcata cggcggtcca cagtgtgaaa
    35461 ccaagtctct aacgattttt ttaaacaaaa agctaataaa gcagataaaa aagcggataa
    35521 gacaatgtga aaatactgca atgaatctcc tggttcaata accgaagatt tcaaaacaaa
    35581 aaaatcgccg atgcttgtta agtgatctcc taaaagcgac gtcaataacc caaaaggaag
    35641 cgcgtaaacc aattgttgac gcgaagtcga aaagaagaaa aaagtaagca tcagcattcc
    35701 tatagcaatt agagcagtaa aaatccctaa aatcgaatag acccatccaa tcaaaataca
    35761 agaaatcaga atgactcccg caaacagata tttgtatcgg atgtgatggt caatcaataa
    35821 gatacaaatt gtgtaaatca aatattgcaa cgctatggac cataaaaaca taaagtacag
    35881 ctcccattga tttttaccaa attatagcac aattggcgta ttattttttt aatttttcag
    35941 ggattttgaa ttcagaactc ataccaaagc agcagccaga tacaaaaaag tcagataagt
    36001 tagttaattg tttaatgatt gtttttttca tgaaagattc ctccaaaatt catcaagtag
    36061 tttgtaatag gaagcaacgt agctatttct aaaacaatcc caagtagcaa ataggtagaa
    36121 aagtaagaag ctgagaaaaa taacgtaatg agagaaaaaa ttagaaacat gctgatcgcc
    36181 ttctttaata atgggggacg tttctcgggt tcaatgggat tgattgctgt acctcttgat
    36241 ccatagcgaa gcagtaacaa aaagcagctg attaacaaaa agaccatcat tgggaaggag
    36301 atagtcaatt gcagatgttt catcaccaag ggaacgcccc ataaataaag tagtgtccac
    36361 agcagacaaa cgaattcaga gtcggaatgc aatccataag catatctgcg gatgcagata
    36421 taagaaagaa aggtgattcc tgtttccgcc catgagcctg tcaccagaga aaagactaga
    36481 acgaccgcta gttttccaag attattcaat aaaacttcta agacaaattt tacttggaca
    36541 tacaccatat cttcttccga tttgtctccg ctgaataagg cttctaataa tttgttcaca
    36601 actttatctt tcatctcata cgtacctcca ccacaaattt tatgctaaaa aaaataaaaa
    36661 acgtcttttg tttcataaaa agtggttttg acatcaaaac cgtcatgatc tcctaaaaaa
    36721 atcttcgttt tccaagaacc ttaccaaaaa aagtgacagt tttgatacga aaagtacttt
    36781 ttatgacaag tttgtgcttt ttcgagtgat ttatgttaaa aaattactga ccttataaaa
    36841 ggtgatggag ggattcttat ggaaaatgaa caattgtttg aagtcggcga aacatatgca
    36901 tttaaatctg atttatttga atcaccaata aaaggaaaaa tagaaaaaat ctatgaaaat
    36961 agtgtgatga tacgtgtcat gaaatgcgca gtagaagaca atgataccgc tcataatctg
    37021 cactggcagt tagttgtacg caaatcaagt acaatagaag aataaaaaca gataaaatgt
    37081 tgagagcata aagaggttgt gagtctcccg tttagctctg agttacaagg aataatgtta
    37141 aaaaacagct tttcatgttt tatgaatatt gccttgtgac cgaggtgttg gcaggagaat
    37201 actgtttatt tgaaaaaagg gctgcgacaa gacttttgtc acagccttta ttttcatgaa
    37261 taggagttac ttttggctac ttttagagaa gtagctttta aggagcagat aaagtcaagg
    37321 cttttatttc tataaatgaa gtagtaaaat gaagaacaga ttttattaca ggaaggatgc
    37381 cgataattgt gatcattgtt gcaggaacga tcggtgcagg gaaaagcaca ctaactgaaa
    37441 tgcttgccca agatttagaa acaaaacctt tttatgagaa cgtggaagat aatgaagtat
    37501 tgccgctatt ttattctaat ccagaaaagt atactttttt attacagatt ttctttttga
    37561 acaaacgctt tttggcaatc aaagatgctt tcagccatga tgacaacgta ctagaccgct
    37621 ccatctacga agatagtatg ctgtttcatt tgaatgcaga tttagggcgt gtttctgaag
    37681 tagaagtaaa gcagtatgaa ggtcttttag aaacgatgct gaaggagtta gaagaaattt
    37741 cgccacaaaa gaaaccagat ctactggttt atattcgtgt atcctttgaa actatgttag
    37801 cgagaatcaa aaaaagaggc cgggaatatg aacagctaga acaagatcct gaactctatt
    37861 cctactataa agaactcaat cgacgatacg aagagtggta tgaacagttt gatatatgtc
    37921 ccaaaatagt gatcgatggg gacaaatatg atttcgtagc cgatccggca tgtggagaac
    37981 aaatcgtaca agaaatcaag atgcgggcaa agaaaatgac agaagaggca gatgcccagt
    38041 cgatccgcta cactacaaat aacccgaaag caaagggaat ctttacaact aaataatata
    38101 aaaactaagc aaattgctca ctcgcagata tttctctgtg agcagcatct tgcttagttt
    38161 ttttgattca ttgagatcaa atagtgaacc gattcgtatg ataaaattgc cataaatgaa
    38221 atgtctgagt gagggccatg atcaaaaata aaatggaaac gatcaaaatc gaatgggcgc
    38281 caaacagatg atgacagaaa acaaggacaa tagctcctgc gataaaggca agaacgacac
    38341 ttagatagtg gaaaaatcga tcaaaatctg ctttttcttt agaaatacaa taatcatata
    38401 agttcgttgc tacccctttt aaatttccag ttgtaaataa attagtgtag gcacgcccgt
    38461 tgattttatc aaaagctacc cactggatcg atgcagtaaa agcaatcaaa atcgttacta
    38521 atgcgggatg ctcatggacg ggtgtcaaac taacaaagaa aaaaatcact gcttcataat
    38581 atagaacaaa caagcgccag aaatgttttt ctttttttct aaaataatca attagatact
    38641 ttgttagaag aatccctaag aaaaagaaaa gggtagacaa tactttttta ccaacactta
    38701 gccattctcc gtcaaatgct tggatgatcg atagaatgat attgccagtt tgagcagagg
    38761 caaaagcacc atattggata taagtataac tgtccatggc tcctccgata aatgtcagca
    38821 aaagtcctat tgtacgcccc tcgtgaaagg aagtctgcat ttttttcatc accttttatg
    38881 aatatttttg tcttccttat cttagcataa acgattgaaa aaaaggaaaa attttcagaa
    38941 aactaaaatc atatcgcttc ccattttcat ttttagaaag gtctgttagt ataaagaaaa
    39001 aatagacaac gaaactttaa ataaccaact tgaaataaac aaattcaaag aagaaagatg
    39061 ggaaggaaat caatcagttg aatgaaaaag aaattctccg attacaaaaa gaattgaaac
    39121 gctgggagaa caagtataaa aagcttcaac aagtctttcg gctacaagaa gaaatcattg
    39181 ctacctatga agcggcttat ggcgaagcac ccggacttat tgaggaagaa gtacaagtaa
    39241 aagaaagtga aggaaacagc tctttcttta aaatgatgga gaaacgttcc tataatcgct
    39301 tcaatcaaga acaaaaagca gctatcacat atgatatgaa gaaacatctt cgaatcatag
    39361 caggagctgg gagcggcaaa acgcaaacaa tctgtgcaaa agctgtttat ctgatgacgc
    39421 aaaaacaagt agacgaagaa cgcatactaa tgatcacttt tacaaggaac gctgcaaatg
    39481 aactaaaaaa acgagtagat aatttcagcc agagaaaaac cgctgtccat atcggaacat
    39541 tccatagtat cttttttagg atatacaatg aaatttgtcg aaaatttcct gaagtagcaa
    39601 tgcaaggaat acaaggagac ttttcaaagg atactgcaca aaaagtgaat gctgttttgc
    39661 aacagctgat acgtaaatat aatctttata tatttgatca atatggagaa aaaacgattg
    39721 cttctcggtt agactattgg cagaatatga acctttcagt agaagaaatg gttcagttag
    39781 ttaaagaaaa atatgattcc attgataaga atgcacgaca gccgatatca gaacgactgt
    39841 atggattgct cacggaactc caagaacaaa aaagaagcca gcaattgcta gagttcaatg
    39901 atgtactcca aaatctgaaa agcgctttgg agaatgagga gatccagcat tatatcggcc
    39961 aaaaatatga ttatctattc attgatgaat ttcaagatac taatccactt caatgggaaa
    40021 tcgtgaaacg gatgacaaag gaaaacagga tcaagttgat tgttgttgga gatgatgatc
    40081 agagtatcta tggattcaga ggatcagagc cagcatacat caagaacttt gaaaaagaat
    40141 atcctaccaa aactcttttc ttattaacta attatcgttc tcgggcagct attgtccaag
    40201 gagcaaatag attgatcagc tataataaaa atgatcggat accaaaggcg atgattccag
    40261 cacaaaaaga agcaggtgtt atggaagcct atttgttttc tgatactaaa gcagaaagtc
    40321 agtggctaat caaacaaatt cagacattta ttcaaaaaaa tgggaaatat aaggaatcaa
    40381 tcatattata ccgatcagct tcacaaacgc agcagcttgt ccaatctctg ctgaaaacga
    40441 atattccttt tgtactggaa gcagatagtc catatgaagg tatctttggt atcaagtctt
    40501 tccaacattt ctatcaaaaa attgttcatt ggcaaacagc actgaatctg caaaagaaaa
    40561 atcaggccta ccagcaattg ctccggcagc tgatggcaga ctgctacctg aaaaaaagtg
    40621 aatgcgatca atacttttct cctaaatatc aaaatatatc cgttgcagat tatattttgt
    40681 cgataagccc caatttaaaa tcgaaatctg cagaaatccg agagtttgag gaagcgttgc
    40741 gtaaaacaga ccagcatcaa gaaatctatt cgcttgttca aagctatctt catctgccac
    40801 gtatagctaa ggaaatcgac cagagtgatc aagaatggat acgagaagaa gtaaagcagc
    40861 atgctacttt cagaagactc caagccttat cgcaagaaac ccaagcgaca gctaaagaat
    40921 taaaaacccg cttgattgct tacagacaag gaaaacttga tgcactatgt atccagagca
    40981 ttcacaaaag taaaggtctt agttaccgaa atgtcttttt gattggttgt aacgaaggaa
    41041 gtttgcctta taacggagcg acagaaaaga aagtaatcga ctttaatgaa ataaaagcag
    41101 aaccagtaac gacgatagaa gaagaacgaa gactatttta tgttgcgatg actagagcaa
    41161 gaaatcgtct gtatctatgt gtacctcaaa tgaaaaattc aagaaaattg aaagtatcga
    41221 gatttatcaa agaaaccggg agtcgtgtga aaaaaggaaa gtgataga
//
LOCUS       DAFEOM010000017        40816 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.017, whole genome shotgun sequence.
ACCESSION   DAFEOM010000017
VERSION     DAFEOM010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.017"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            10..588
                     /locus_tag="LP524_001067"
     CDS             10..588
                     /locus_tag="LP524_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234136.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(828..911)
                     /locus_tag="LP524_001068"
     tRNA            complement(828..911)
                     /locus_tag="LP524_001068"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(875..877),aa:Leu,seq:gag)
     gene            966..1145
                     /locus_tag="LP524_001069"
     CDS             966..1145
                     /locus_tag="LP524_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234137.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            1294..2511
                     /locus_tag="LP524_001070"
     CDS             1294..2511
                     /locus_tag="LP524_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBL2234138.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            2695..3531
                     /locus_tag="LP524_001071"
     CDS             2695..3531
                     /locus_tag="LP524_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2234139.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     NEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            3607..4755
                     /gene="nagA"
                     /locus_tag="LP524_001072"
     CDS             3607..4755
                     /gene="nagA"
                     /locus_tag="LP524_001072"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBL2234140.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            4976..6034
                     /locus_tag="LP524_001073"
     CDS             4976..6034
                     /locus_tag="LP524_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBL2234141.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            6038..7072
                     /locus_tag="LP524_001074"
     CDS             6038..7072
                     /locus_tag="LP524_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HBL2234142.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(7174..7890)
                     /locus_tag="LP524_001075"
     CDS             complement(7174..7890)
                     /locus_tag="LP524_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBL2234143.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(7859..8644)
                     /locus_tag="LP524_001076"
     CDS             complement(7859..8644)
                     /locus_tag="LP524_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HBL2234144.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(8635..9384)
                     /locus_tag="LP524_001077"
     CDS             complement(8635..9384)
                     /locus_tag="LP524_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBL2234145.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9682..10206
                     /locus_tag="LP524_001078"
     CDS             9682..10206
                     /locus_tag="LP524_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBL2234146.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10222..11721
                     /locus_tag="LP524_001079"
     CDS             10222..11721
                     /locus_tag="LP524_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HBL2234147.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(11809..12411)
                     /locus_tag="LP524_001080"
     CDS             complement(11809..12411)
                     /locus_tag="LP524_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL2234148.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            12515..13471
                     /locus_tag="LP524_001081"
     CDS             12515..13471
                     /locus_tag="LP524_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HBL2234149.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(13776..14732)
                     /locus_tag="LP524_001082"
     CDS             complement(13776..14732)
                     /locus_tag="LP524_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL2234150.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFVSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            14906..15466
                     /locus_tag="LP524_001083"
     CDS             14906..15466
                     /locus_tag="LP524_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234151.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15459..16283
                     /locus_tag="LP524_001084"
     CDS             15459..16283
                     /locus_tag="LP524_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBL2234152.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            16293..16910
                     /locus_tag="LP524_001085"
     CDS             16293..16910
                     /locus_tag="LP524_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBL2234153.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            16928..17611
                     /locus_tag="LP524_001086"
     CDS             16928..17611
                     /locus_tag="LP524_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBL2234154.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18294..19577
                     /locus_tag="LP524_001087"
     CDS             18294..19577
                     /locus_tag="LP524_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL2234155.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            19593..20480
                     /locus_tag="LP524_001088"
     CDS             19593..20480
                     /locus_tag="LP524_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL2234156.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20497..21333
                     /locus_tag="LP524_001089"
     CDS             20497..21333
                     /locus_tag="LP524_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL2234157.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21336..21959
                     /locus_tag="LP524_001090"
     CDS             21336..21959
                     /locus_tag="LP524_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234158.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            21956..23248
                     /locus_tag="LP524_001091"
     CDS             21956..23248
                     /locus_tag="LP524_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL2234159.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      23607..23704
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            23820..24401
                     /locus_tag="LP524_001092"
     CDS             23820..24401
                     /locus_tag="LP524_001092"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBL2234160.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24404..25711
                     /locus_tag="LP524_001093"
     CDS             24404..25711
                     /locus_tag="LP524_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBL2234161.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26127..26615
                     /gene="purE"
                     /locus_tag="LP524_001094"
     CDS             26127..26615
                     /gene="purE"
                     /locus_tag="LP524_001094"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBL2234162.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            26608..27732
                     /gene="purK"
                     /locus_tag="LP524_001095"
     CDS             26608..27732
                     /gene="purK"
                     /locus_tag="LP524_001095"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBL2234163.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            27745..29040
                     /gene="purB"
                     /locus_tag="LP524_001096"
     CDS             27745..29040
                     /gene="purB"
                     /locus_tag="LP524_001096"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBL2234164.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(29220..29597)
                     /locus_tag="LP524_001097"
     CDS             complement(29220..29597)
                     /locus_tag="LP524_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234165.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            29853..30581
                     /locus_tag="LP524_001098"
     CDS             29853..30581
                     /locus_tag="LP524_001098"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBL2234166.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            30581..30835
                     /gene="purS"
                     /locus_tag="LP524_001099"
     CDS             30581..30835
                     /gene="purS"
                     /locus_tag="LP524_001099"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HBL2234167.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            30837..31508
                     /gene="purQ"
                     /locus_tag="LP524_001100"
     CDS             30837..31508
                     /gene="purQ"
                     /locus_tag="LP524_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HBL2234168.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31509..33731
                     /gene="purL"
                     /locus_tag="LP524_001101"
     CDS             31509..33731
                     /gene="purL"
                     /locus_tag="LP524_001101"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HBL2234169.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            33716..35155
                     /locus_tag="LP524_001102"
     CDS             33716..35155
                     /locus_tag="LP524_001102"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBL2234170.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            35178..36230
                     /locus_tag="LP524_001103"
     CDS             35178..36230
                     /locus_tag="LP524_001103"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBL2234171.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            36227..36805
                     /gene="purN"
                     /locus_tag="LP524_001104"
     CDS             36227..36805
                     /gene="purN"
                     /locus_tag="LP524_001104"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBL2234172.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            36802..38337
                     /gene="purH"
                     /locus_tag="LP524_001105"
     CDS             36802..38337
                     /gene="purH"
                     /locus_tag="LP524_001105"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBL2234173.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38353..39627
                     /gene="purD"
                     /locus_tag="LP524_001106"
     CDS             38353..39627
                     /gene="purD"
                     /locus_tag="LP524_001106"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBL2234174.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            39749..40663
                     /locus_tag="LP524_001107"
     CDS             39749..40663
                     /locus_tag="LP524_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBL2234175.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
BASE COUNT        13052 a         6975 c         8971 g        11818 t
ORIGIN      
        1 tttttagaca tgaatatctt atttttattg ctatttttct taattttcct tctaacatat
       61 aggaaatgtc aaaactatta tgtgcgtata ttgactgcat taccgttttt cttaaacctg
      121 attatttatt ttggtaatac aatgggtcaa agctttactt acgtcaatgg aaataaaagg
      181 gcaatgattt ggagtagtag taacctgaat aatctcttta cagagttagg aacgaaactg
      241 tcactttttt atccaggtac ttggatagct actttagtag tcttggcatt attactatgt
      301 ttaattgttg gaatttattt aagttttgac aataagaaaa cttctatttt tttagttatt
      361 ttgatgataa tgggattttg ttcaaggtta attatgggat tttctcccac agtatgggct
      421 tccggaatgc ggacgtacta tatactttat gttgttatcg ccatcttagt actaatggca
      481 gttaaagagc taatgaaaag catgagtgta caaaaaaatg aatttatgca atttggatta
      541 accgtgcttg gtatttgtac ctttataata acggtgataa acagataata tacaaaaaat
      601 ccctcgcttt agtattaggt gagggatttt actcgttttt cgattaattg ttttaacact
      661 tgcaagtttc tacgagtagc ttgtttccga ccttcatata ctcacctcag gctgtttaca
      721 tgattgtacg ttctttttgt aaaggataca gtgatatgta tataatggca taaagaaacg
      781 ttcaaacaaa aaaactcgtg aagcaaaatt aacttcacga gttgtagtgc agatgatggg
      841 actcgaaccc acacgagcta acgctcacat gaccctcaat catgcttgtc tgccaattcc
      901 aacacatctg cgtaataata acacactaat gatagcgagg ttttacatag ttgtcaagct
      961 caaaaatggg aggacttatt aagatggaga aatatgacgg agaattttca ggactgggaa
     1021 tgatcttagg tgtattgatt ggacttgcat tcggacgttt tttgcttggt tttatgctag
     1081 gaatcatttg tggaatcgca atggattggg cagcgaatct atggaatgac tatcatgaca
     1141 attaagttct caacgcttgt ttgtgttttt ttaatactta cgtttttcct aaataagtgg
     1201 caaaattttc taggatggag tatgatattg aaatagtaaa taacaaaaaa tttggaaagt
     1261 tctaactttt tgaaatgagg gattggctaa gtgatgagag aatttgaaaa atccaacaaa
     1321 ttagaaggtg tcagttacga tgttcgcggt cccgttctag aagaagcgga gcggatgcaa
     1381 gaagagggca ttagtatttt aaaattgaac acgggaaatc ccgcaccttt tggatttgaa
     1441 gcaccaaacg aagtcattcg tgatttgatc atgaatgccc gtgactcaga aggctactca
     1501 gattccaagg ggatcttttc tgctagaaaa gcaattgagc aatattgcca actgaaacat
     1561 tttccgaatg tgacgatcaa tgatatctac accggcaacg gagtgagcga attgatcacg
     1621 atgtgtatgc aggggttgct ggacaatgga gatgaagtac tggttccaat gccggactat
     1681 ccgttgtgga cggcttctat tgcattagct ggaggcacac ctgttcatta tatttgcgat
     1741 gaagaggcag aatggtatcc tgatattgat gatatcaaat caaaaataac gagtcggaca
     1801 aaagctattg ttatcattaa tcctaataat cctactgggg cactttatcc aaaagaatta
     1861 ttagaagaaa tcgttgaagt agctcgacaa aataacctga ttatttattc cgacgaaatc
     1921 tatgatcgct tagtgatgga tggtctagaa catattccga ttgctacatt agcgcctgat
     1981 ttgttcgttg ttacgctgaa cggtctatcc aaatctcatc gtgtagcagg atttcgagta
     2041 ggctggatgg tcctaagtgg agataagtca cacgtcaaag gctatataga agggctgaat
     2101 atgttgtcct ccatgcgttt gtgttccaat gtattgtctc agcaaattat ccaaaccgcg
     2161 ctaggcggtt atcaaagtgt cgatgagctg ctcctgccag gcggaaggat ctatgaacaa
     2221 agagaatata tctataatgc aatcaatgat attcctggtc tttcagccgt aaaaccaaaa
     2281 gctgcttttt atatattccc aaaaattgat acaaagcgat tcaatatttt ggatgatgaa
     2341 aaattcgttt tggacttttt acatgaacat catattctac ttgtacatgg tggtggattt
     2401 aattggaacc agccagatca ttttcgaatc gtttatcttc ctaaaatgga tgatttgaaa
     2461 caaacagcta agaaaatgag agaattccta gcaacctatc gccaaaaatg attttaaaaa
     2521 ccaaattttt cagtaggagg gccacagaca aacacactga ggctctcttt ctagtggagt
     2581 tttcttaaat aactaaagta tattatttag ttgcaagtct tttcttgaag tttgcgactt
     2641 cctaaagtaa aatacgaata gaatgcgaat acgcatattt tggggaggat acttatggaa
     2701 acgttaatcg agtcctttgg aacgatcatt aaagagattc gaaaagaaca aaaaatgacg
     2761 caaaaaatgc tttcgcatga tatttgttca caaagtgtat taagtcggat cgaaaataat
     2821 gaggaactgc cgaatgtatt ggtgatgatg cagatttgtc aacggctagg ggtaacgatt
     2881 gatcatgtga tgaatctctc tcgaaaaact actcggacaa acgataaatt atttgaactg
     2941 ttggatagtt gtttccaaaa acgtgattat cggaagttag aacaagtact aaaatcttct
     3001 gatatttctg aaaatctgta tcaagcaact gactttcaac gctattatta ttatctcggt
     3061 gtttgcgaat tcacgttgcg tcaaaatatc tctcagtccc tttattattt gaaagaagca
     3121 ttaagctact ctagccaaaa agaaagaatc catgtttctg acacagaaat acaactgatt
     3181 agttgtatcg gaaaaatata tggtgtggca ggaaaaacgg ctgaagcacg ctactatttg
     3241 agtcgaagca tccaactgct gcatcaatca gctgatgaac gatcaaaaag tgaattttcg
     3301 caaatttttt ataattatgg aaatttctta ttccatcaaa atgaaatcaa cgaagcgtta
     3361 gaacaggtga atcaaggaat ctattgggct caagagaaga atagctacta ttacctcaat
     3421 gatttatttg tgcttaaaag cttaatatac aagcgtaaag aagaaccaga aagagcagca
     3481 ttctatgaag gcttagcaca agctgtgaaa caaatttcaa aaaatttgta aatggtgtag
     3541 accattcttg acttcttctc taaataagag tactttagag gtagaaaaac aaagggagag
     3601 aatagaatga gaacttttat ctatgctgac aaattctttt tagcgtcagg tgtcaaacat
     3661 gcaggctatc ttgaaattac cgatggactt tttggtgaat acaccaaaga actacctgag
     3721 ggagaagtca acatcattga ccaaagcggc aaatggattg ctcctggttt ggtagataca
     3781 catattcacg gctatatgaa tcatgatgta atggataatg atgcagaagg catcaaagtg
     3841 atgtctgaag gtctgttatc ttgtggagtg acttcatttt taccaacaac attgacgtca
     3901 agcaaagaac gtttaaaaga tgtagctgag acaatcggca aaatgtatca ggaagcacca
     3961 ggagctaaaa tcaaaggaat ctattttgaa ggtcctttct tcacagaaga acacaaaggg
     4021 gcacaaaacc caagttattt cggggatcct gatttggata ctttccatga atggcaagaa
     4081 gcttcaggtg gtttaatcaa aaaaattgct ttagcaccgg aacgtcaagg tgtagaagag
     4141 tttgtcaaac aagtgactga agaaggagta gttgtttctc taggacacag taatgctaca
     4201 ctagaagaag cgcaaaaagc tgttgaagca ggcgcaagtg tatttgtcca tgctttcaat
     4261 ggtatgcgtg gattgaatca tcgcgaacca ggtatggtgg gtgcgttatt atctttagaa
     4321 catgtctttt ctgaattgat ttgtgatggc catcatgtac atccaaatgc tgcgggaatc
     4381 ctaatggaaa aagcaggaca tgatcatgtc gcattaatta cagactgtat gatggccggt
     4441 gggatgcctg atggaaatta caatttaggc gaatttccag tagtagtaaa agaaggaaca
     4501 gcacgtctag aatctggcaa cttagctgga agtatattga aattgaaaga agcaattaaa
     4561 aatgttgtag actggggatt agcaacacca gaacaagcag tcatgatggc tagtctgatt
     4621 ccagcaatca gctgtaaaat cgatgataca tgtggaaaaa tcgctcaagg acgcgatgca
     4681 gactttatcg tattgaaccc tgatatgaca ttggaagcaa cttatctaga tggcgtggaa
     4741 cgtttccacg cataatataa tttaagttaa ccttaaaact aaacttctta agtttggttt
     4801 taaggttttt tttgtgttct agtaaaaatc actttacata agtttcaaaa gtttctttaa
     4861 tttcctattg ttcaataagt aacaatgtaa agataaaatt aaaaatgtta atacgtatta
     4921 gcaaaataaa agcttttttt attgtgaata aatgaacata attaggagga gaaagatgaa
     4981 cttatttgac atttttcgat cgactttgac agatatgtcg attgtcagtg caatcacttc
     5041 aacagttttt attatattat tagggtttgt ttgtagaaaa aaggggattt tttctgaatc
     5101 tgttggaaaa attctatcga aagtcgtctt aacagtggct ttgccagcac tggcatttcg
     5161 agctttcatg caagatatca atacagaatt actgatgcaa ggaatcaatg tcttgatttg
     5221 gggattagtt atttatatta ttcttattct agtttcaaaa ccttttttct taagctataa
     5281 aggggataag caaacaacgt tgcgtgtcct gacgatcttt ggttctacga cctttttcgg
     5341 aacgcctatc gtcagtgcga tttatggtcc tgtcggcgtg atgtattcct caattttcaa
     5401 tattggctac cgaatctttt tgtattctta tggatacata aaaatgagtg gcttgaagat
     5461 ggaagctaaa aatatcaaag cgatgttcgt caatccaatc attatcgcta cttttgccgg
     5521 ccttttcatt tggctgttcc aagggtatct gccacaagtg tcagttacag gtgcagaagg
     5581 agagataagc caagtagcgt ttttgcgaat tgaccagact gcaccgtggc tatttcagcc
     5641 gatggattat ttagctggtt taagttcacc attggcgtgg ctagcaatcg gcgcaacatt
     5701 aggtgaagta agcttcaaaa aagctgcatc tgaaaaaact tcatggtatt acagtctagt
     5761 caaagtggta tttgtacctg cattgaatat cgtgattcta gcagttttga cggcaacgaa
     5821 tattctacca gtcaattttg aagcactggc aacaatcgtt atcatgatgg ctacaccagc
     5881 tgcaacagtg gcagcagcct atgctatcag ttttgatagg gaggcacttt tagcctccaa
     5941 tgcatcctta ctgtcaacta tttttgctgt ggtactgaca cctgtatgga tcgttgtatt
     6001 acaaatgatt catggcatag gtttattttc ataagaaatg gaggaagaaa gtatgagaat
     6061 cgcatgttac ggtgttcgtc cgaacgagat agattttttt gaaaaattga atatttacca
     6121 ttatgatctc agtctttatg aagaactgtt gacacatgac aatatcgtgt cagcaaagga
     6181 ccatgatgct gtattgttga ggggaaattg tatagcagat gagaccaatc ttgcaaaaat
     6241 gcaggaatat gggattcgtt atgtttttac acggactgtg ggagtcaatc atatcgatct
     6301 acaagcggca gctgattttg gtatgacagt agcccgtgta ccatcgtatt cgccaaatgc
     6361 gatagcagag ttatctttga cttttgccat gatgcttttg agaaatactg cgtatacgac
     6421 gatccgaaca tctttcaaag attttcgagt ggatgagcaa atgtttagtc gtgaaatcag
     6481 aaattgcaca gtagggatca taggaacagg caggataggg ttgacggaag ctgttttgtt
     6541 taagggacta ggagccaaag tgatcggcta cgatattcat cagacagaag ctgctaaaaa
     6601 agtattgacg ttccactcgc ttgaagagtt attagccaaa agcgatattg tcagtattca
     6661 tgtacctcat atcccaggtg aaaacgatca aatgatcaat gaaggatttt tagctcaaat
     6721 gaaaaaaggt agcatcttga ttaatacagc ccgtggagag cttcaagata atgaagctat
     6781 tttaagagct ttgaaaagca atcatttagc tggttttgca accgatgttt ttgcaaatga
     6841 aacagaggtt tttttccgat ccttcaagcc atgggagacg attccagacc ctgctatcca
     6901 gcaattagta gaaatgtacc ctagagttct tgtaaccccg catgtaggtt caaatacaga
     6961 tgaggcatta tcaaatatga tttcgacaag tttcgagaac tttcgtgaaa taatagaaac
     7021 tggaaaaacg aaaaatgagg tatctcttcc taaagcccgt caattaagat aagaaaacat
     7081 ggatattcgt caataaaatg tttaaaaata gtccaagtaa acagttgtta cttctgtata
     7141 cttggactat tttgtatggg ataattcgat gactcaacag taatctgtga gctttttccg
     7201 cccattcttt gtcaattcat aacaggttag cggccggccg atttccaaat atttgattct
     7261 ttcgttcaat atatcttgtt ctactaaaaa attcaaatat ttcttgacgg atatacgtga
     7321 cagtgagctt ttttgagcca gttcttttgt tgaaaaaata gtatcttctt ttagaataaa
     7381 tgcacttatt ttttttaaag tcagttttga cagaccttta ggcaaatcga tgaatgatgg
     7441 tatttcgtga atgggagtgg tgtggttgaa tacttgatcg atcaatttct gatcggcttt
     7501 ttcagtcgtt tggattgtct gagagaaagc aagatacttt tcgatcgcca gctgaaaacg
     7561 ttgcaaagtg aacggtttga tcaaataatc taaggcaccg tagcggatca cattctttat
     7621 ggtttcgata tcatctgcag cagtgatcaa aatcactggg atatcatttc cttgctgtct
     7681 aagccaagat aaaaaatgga taccactttc gccaggcatg tatacatcaa gcagcagcag
     7741 atctgcgttt ttttgtctga aatatttttt tccttcttct acatttctga aactttcgat
     7801 tacgttcata ttagggatgc tttccacaaa agtacgattc aattctgcaa ccattggatc
     7861 atcttcgatt atcaggacat ttgtcatcat tttgatcttc cttttttggt aattctagat
     7921 agacgattgt accgccattt gtttgtttgg aaaattctaa aaatccaccg tggcgtttga
     7981 cgattgaatg gatgagattc aatccaaacc cacgattgat tcccttcgtc gaaaaaccta
     8041 gcgtaaatat tttttctgag atttcttctg atatcccgac accgtcatcg cagacggtca
     8101 cgatgaatac ttcagcttct gtgtcatagt ttaaatgaat ccggacctgt ccgtttcttt
     8161 catgtttttg tagcacggct tcttttgcat tatcaagtaa gttccctaac acttgcaaaa
     8221 gtgaatggat ggagtcatcc atctccattt tagggataaa agaatcgctt tctaacagca
     8281 tttcaacaga ttgttctttg gcttcgttta ttttgcctaa taaaaaaccg gcaattgcag
     8341 gtactttgat cagacttgag acttggccaa tctctttttt ataattttga tttagctgct
     8401 ggataaaatg ggtgacttct tcatacttct tcaaatcaat caaaccagaa atgacatgaa
     8461 gtttattcat gaattcatgg gtttgagctc tcatggagtt tatatattgt ttcgtccccc
     8521 caagctcctc cattaatttc attacttctg atcgatttcg taaagttgcg actgctccta
     8581 aaaaacggtc attgctaaaa agaggtgtaa cgctagaaat agtttctatc ccatttaaat
     8641 acatggattg agtccgaacg gtcgtttgat gctcaatcgt ctctttaaac aaaacgtcat
     8701 atagattatc actgattttt ttcctacttc gaaatccaat agctgctctg tttggtagaa
     8761 aatgtttttt gcaggtgtat tgatcaagag gatagtttta tcagaagaga tggctaagat
     8821 tccttcatct acttcatttt cgataatcgt tttttcactt aactgttctg ctatttcttg
     8881 tggttcaagt ccaagcagta cttttttcat tttttgggca agaaaaacgg cgcctatgat
     8941 gcctacgcta agtccaataa tcaatgaaaa aacaatcatt ttttgtccat ctttgacttg
     9001 attgtcaatc gtgttgtttg ttaagccaac acaaactgca ccaataatct cttgatcata
     9061 ataaataggg acgaaatagc ggaagccgaa ccctaacatt ccttcttttt cagagaaaaa
     9121 ttcttgtcct ccaatcactt ttttcacatc ttcatgactt gagaaaggaa gaccgatgta
     9181 acttgggtct gcgtgagaat atcgaatgcc ttcagggtcc atgacaacga taaaatctaa
     9241 attcgatgca atcatgattt catttgttat tccttgaatc gcatgtgtcg ggtcaccagt
     9301 ctgcagcttt ttttggatga gtttatcatt ggcaacgatt ttagcaatgc tagagatttt
     9361 atctgacaca ctagatcggt tcatcgaaag aataaaattc ctgatcaata tggcagaaac
     9421 aatcaaagaa acgaatgtgg agaaacaaac gacaaggatg atcgtgttct ggagctttaa
     9481 tggttttttc ttaaaaaaag gaaacatagg ctacctcgaa agaatttatt gtgaaaattt
     9541 taacatgaaa acaaaaattt aacaaattgt caacgataaa cacaaccttc ctataaaaaa
     9601 caccactgag tcggtaaaag aagctataca gaattttcaa acattggtat gatagacgta
     9661 gaaatggaag gaggcgtgta tatgataggg ggaatcgcac ttgagacgat ttattggtat
     9721 acattgctga tttgtgcagg attggcagtc ctattgatta tttttggaga tgtattcgat
     9781 tttgatggcc cgcttgatcc gatgcttatt atcccttggt taactttcac aagcttattt
     9841 ggttttctag gtgagcgttt aacagatcaa tcatctttat ttttatttat tgctagtgga
     9901 ggcatatcga tagtgttggt atttttgctt aatttctata tattgatgcc gatgaaacat
     9961 gccgaatcaa cgatgtcgtc ttctgaaaaa actctggaag gacaagtagc tgttgtgatc
    10021 acaccgatcc ctgtacgagg tatgggagaa attcagttga aaagtgttac cggttccatt
    10081 agtcgtccag cttgttttta cagcccccaa gaacagacag ctgagcgggg aacgaaagta
    10141 ttgatcattg agatcaagga aagaatctgt tatgtcatgc cttatgaaag catgataaaa
    10201 gcttaaagga gcgtgtgtta aatggaaata ccagttgttt ttatcgcgat cgcaatcgca
    10261 gctttgattt tactgatgtt gctgatcgtc tttgtagcga agtaccagac agcaaaaccg
    10321 gatgaagcgt tgatcatcag tggaagttat ttaggaagca aaaatgtgca cattgatgaa
    10381 agcaataaca aaatcaaaat tgtgcgtgga ggtggcgcat tcgtcttgcc agtttttcaa
    10441 cgttcgaatc ggatcagtct attatcgagt aaattggacg tctccacccc tgaagtgtat
    10501 acggaacaag gcgtaccggt gatgtgtgat ggtacttcga tcatcaaaat cggttcatct
    10561 gtagaagaga ttgctacagc agcagaacag tttttaggca agacaagaga agaactggaa
    10621 aatgaagcaa gagaagtatt agaaggacac ttgcgctcga ttcttggttc tatgactgta
    10681 gaagaaatct atcaaaatcg cgataagttc agtcaaagcg tacaagaagt cgcttctgta
    10741 gaccttgcaa aaatggggtt gatcatcgtt tctttcacta tcaaagaggt cagagacaaa
    10801 aacggttatc ttgactcttt agggaaacca agaatcgccc aagtcaaaag agatgcagat
    10861 atagcagaag cggaagctct aaaagaaacg cgtatcaaaa aagctcaagc agaaaaagaa
    10921 tcacaagcag ctgaattaca gcgacaaaca gagatcgcag aatcattaaa agaaaaagaa
    10981 ctgaaattag caacctacaa acaagaacaa gatgttgcta aagcaaaagc agaccaagcc
    11041 tacaatttag aaagtgcccg agcacaacaa caagtaatcg aacaggaaat gcagattaaa
    11101 atcgtagaac gtcaaaaaca aatcgaactg gaagaaaaag aaatcacacg tcgtgaaaag
    11161 caatatgatt cggaagtgaa gaaaaaagca gatgccgatc ggtacgctaa agaacaagaa
    11221 gcacaagctc aaaaagtcaa agaagtgact gaagccgaag cagaacgatt ccgtgtggaa
    11281 gcactggcag aagcggaagc caataagaca cgtttagctg gacaagcaga agccgaagca
    11341 gcactagcaa aaggaaaagc agaagccgaa gcaaaacaaa agatcgccaa tgccttcaag
    11401 gaatatggcg aagcagctgt cttgagtatg gttatcgata tgctaccgca attgatgcgc
    11461 gaagcagcac agccgctagg aaatatcgaa aaaatctctg ttgtcgatac aggaagcagt
    11521 tcaggagaaa ccagtggagc gaaccgagtg acaaattatg ccacaaattt gctttcttct
    11581 actcaagaaa cactaaaagc gacaacagga cttgacttga aggaaatcgt agagaatttg
    11641 tcgacacgtg ggactactag ccaagtaaat atgtatgaag cgccaaaaga ggcagaacaa
    11701 aactcgatga aggaagaata aagtagtaca cttcaccgtt caatcaaaaa tggaggaaga
    11761 ctttttaaag ttcttctctc cattttttta ttttattgca aaagaaaatt agtcctcgct
    11821 atttttcttt tgtttttgga tcttagcttc tatctcttgt aaatttgttc ttagcaatcg
    11881 ttctttcttt tggagttcga ccaaatcttc tgatagttct tccttctttt ctgtttcagg
    11941 aggacgaaga aagaaattga taccgattgc aatgaatgtc cctataaaag tatctagtac
    12001 acgttggata gcatacaaag cggattcgcc ttgagggatg ctcaacgcaa tcagcaacaa
    12061 cgtagctatt gcggaaatga taccggaatt attgttaata ccatctgaca cgacgatcac
    12121 aataataact agaataggaa gcaagatcaa ttctaccaaa aaatcattgt gaaaatagct
    12181 tttgacaaaa aaataaatga tagctaatgt accacctaat gtattgccga tgatcctaga
    12241 ctttccaaaa gaaacggttg tagtcaagtc ctgacgcaaa gaaaaaacag cagataatgc
    12301 agcaatcatc gggattcctc gttgagtcaa atgaaaaaac aagatacata aaaaaacagc
    12361 taaggcactt ttgatcgtac gcatacctag tcgaaaacgg ccaaattgca tagagattct
    12421 cctttttggt ctttttacta tcatacctaa acttttgtct gatagctagt ttttcattat
    12481 tcaagtataa ttaaaataaa aaagggggaa gtaaatgcgt ataggaatgc gaacagtcaa
    12541 aacagtgatc agtgtcttct tttcgatggt cacagcgggt agcttatcgc tgttgtattg
    12601 gccagctgca gggatcattg cattattaag tgtaggaaat accaagcgct ctacattgcg
    12661 tacgggcatg aatcgaatcg cagcctttat attagctacg gcaacagcac tgttcagttt
    12721 ttttgctgtt gggtatacta tttttggctt cagtcttttt ttattcctgt tcatcccgct
    12781 tgcctctcgc ttcaaattaa ccgaagggat agtagtaaat tcggttttgg tcactcatta
    12841 tttagtggag gaaaacatgt cttggcagct gatcggcaat gaagcgggat taatggcgat
    12901 tggattagta tttgctttgc ttgcaaatgt ttacatgcca gatcgtacga agcagctgaa
    12961 ggaaaaccaa atacagatcg aaaaagaatt tcgaaatatt ttgagacaaa tggcagaatt
    13021 tcttttatca gaaaataaag aagatgtgca gatcaaatgc gaacatttac tgacattcat
    13081 tcgcgaaagc caagaagatg ccagagaaca tcaagaaaac tactggctca gacagccgct
    13141 atactacgaa acttacttct ctatgagaag agcacaggcc aatgtgatta aagacatgct
    13201 ggaaaatcta gagagaatcc agcaaccagc ttattatgga aaacatatct atggtttatt
    13261 gatttatact gcagaaacat tttcagagtc aaatgatggc agacaaattt taacacgtat
    13321 tgaagaagtt tatgtattgt atcggcaaat gcctctgccg actagtcgcc ctgaattcga
    13381 ggatcgagca gaattgttcc agtttttgca gtcttttaaa agttttattg aaatcaaggc
    13441 agaattctcc caacaaaaaa tccaaaaata gaggttgtga attcagctaa tattcaggtc
    13501 aaaaagctga tttcacaacc tcttctttaa acggtgttct cctgtcaact cctcggtcac
    13561 aagtcacaat attcaacaaa catgagaagc tgttttttga aattgctcct tgtgactcgg
    13621 agctgaacgg taggctcaca acctctttct taaacggtgt tcaaaagctt tcctctgcgg
    13681 taataagtcc agccaaacga aaaataggag gagctatttt tgtttggctg ttcttattac
    13741 ttgatgcaga acagcttttt cacaaccttt taaaattaca tatttttcca tagaacttgc
    13801 atttgatcca agatttcgat gacatctttt gtcaagaaga gagagcgatt gggcaagtca
    13861 ccttcaatca tgtgtaccat attctttatt tcgtaattca ttgcttcaga tgtattcccg
    13921 ctttcgatga attcctttgt accatctgta aataagatct ccgcacgatc agcacgcggg
    13981 taatcagcaa tcgtcacata accgttctca aaggcaacga taccgacttt tggcattttt
    14041 gcctgaaaag tcaagctgac agcagcaagt tcattttctt tgttacgcaa gatagtgacg
    14101 gattgttcgt ccacgcctgt ttcaaaagga accattgtag aggcaactac ttcaggttga
    14161 gagctcaaaa agaaacgagc gaaagataca gcatacgtac cgatatccaa cagagctcct
    14221 ccagctaatt ctggattgaa gaaccggttt ttagggtctg gttctttgta actgacgaaa
    14281 ggtgcttgga tcatttttaa agcccccagt tttcctgtat ccattattga gcgtaactgc
    14341 tgataaagtg gcatgttgaa gattgtcatc gcttctgcta agatcaaatt cttttcttct
    14401 gctaagcgca tagcgtcagc cagttctttt ttattcatcg tgatggcttt ttcacataga
    14461 acgtgtttgc ctgcttccaa tgctgccaag atatggtcga tatgctgtct atttggtaca
    14521 gcaatgtaca cgatgtcgat ttcagcatcg tctaacattt cttgatagct tccgtaagct
    14581 tttggaatat tgtaacggtg agcgaagttt tccgcttttt ctgaagttct ggaagcaact
    14641 gctgttagtt cgctcgtctc ttgattgaag ctttcggcaa actcatgtgc gatggtacca
    14701 agaccaacga ttccccattg atattttttc atctgaaaac gctccttcat agttggattg
    14761 agtttcttgt ctgtctatta ttataacaaa aatcacgaag aataaagagg agaggacttg
    14821 aaacattaaa aaaaatgaaa gcagcgattt aagaggtgct ttttgatcaa aacttaagta
    14881 gaatagttaa aaggagggga taaacgtgca tcaagtatat attgatctta ttatgagtgt
    14941 attattagaa cagtttgaaa ctgaaaaaaa ttttttagat gaatacttgc tgatcagtcc
    15001 agaacaatgg caaaaatgga aagatggcga gaaaaatctg ccatctgaag cgatgcagaa
    15061 agtaaaaaat cttttcactg attatgaatg gatgctaacg caaaagattt tgcgtcaaac
    15121 gattattttt cccgaaaaac gaaacgttgc cgttgcagag tacaagcaaa tcaaaacacg
    15181 catcgcgcaa aaatggctta gcagcaaagc agcaaaagtc gagctgatcc ctttgaaaaa
    15241 taatcagcag gctggtagat accttgactt gaaagtaagt attcaatatg atgaatgggg
    15301 ctatgatgat attttgaatt ttcgtctgcc agcagatgtt caaaaacaga tcgaaggaga
    15361 aaaaatcgaa cttctcgatt gggtgaatga aaatctggaa gaaacttacg ttcatgaggc
    15421 gaatggggaa cgtgaagaac aacgaaagga tttttcaagt gaataaattt ttaggatttg
    15481 gagcagttgt gattatgatc gcagcaatgg gctatgttat ttttagcgat gatgattcgc
    15541 atgttcaagc cggcaatcaa gcagtcgaac gaacaagcac caaaacaact gctagtaaag
    15601 aaaagaacag tcaaacaacc agcagcaagg taaaggacaa agaaacaaaa gatcgtacgt
    15661 ccaaacaatt gccagatgtt aaaacagacg attggaagct agttttggtt ggtccagatc
    15721 acaaattaga aaaagaaatt gatgagtcta ctcaattggc atcattaagt aacggatact
    15781 tgatcgacaa acgcattgct ccagaatatg aagcatttgc caaggctgct gaagcagctg
    15841 gatttccatt agtgatggtc tcagcttacc ggtctgtatc atcccaacaa caagttttcg
    15901 aacaaaatgt ccaagatgtg atgagtcgtc aaggtgtatc agaagaggag gcgacaaaga
    15961 ttactaaaga aacgattacg gagccaggat acagcgaaca ccacacaggt ctggcagtcg
    16021 atgtcgtcga tcaaaactgg tacaacagtt atccttctac tgtattggat gcaagttacg
    16081 gagatcagcc tggagctaaa tggatcgcag ataatgctgc gcagtatgga tttatcgttc
    16141 gttatccaaa aggacacgaa gatataacta agatcacata tgaaccttgg cacttacgtt
    16201 atgtcggtaa agaaaacgct gagtatatca ccaaacacca attgacgcta gaagaatatc
    16261 tgaatgaatt aaatgaaaag taggaattga tcatggcgaa aaaaaagtgg aaaaagcaaa
    16321 agcgcaagaa tatccgttgg ccagcagtgg ttgccggtgt agcagtgata ctcttagcct
    16381 ttgttttttc cttgaagaat ctgtcttctc cttttactga taatcagcaa gaaaatgaag
    16441 cgcaatccca tcagcaattt attgaacggc ttgttccgca tgcccaagaa ctacaagatg
    16501 gttatggagt gcttcctagt atcattttag gacaagctat attagaatca aattggggaa
    16561 aaagtacctt ggctagcaaa tacaataatc ttttcgggat caaggcttat ggggatcaga
    16621 agaaagtaag cctggaaaca aaggaatttg ttaacgaaga atggataact atccaaggag
    16681 acttcaaagt gtatgattca tgggaacagt ccatggatga ccatacacaa ctgttcgtcc
    16741 aaggagtaga ttggaatcct gcgctgtatg aaaaagtgat tacggccacc aactatcagg
    16801 aagccgcaca agcattgcaa gatgcaggtt atgcgacaga ccccggttat gcgcaaaaaa
    16861 tcatccaggt catcgagacg tatcaattga atcagtatga ccggagatag caaaggagaa
    16921 gtggctgatg gcagaacaat ttacgccaga aatcatgacg gaattgagaa aagatatcgt
    16981 aagagtaccg gaaatcattt tagaagcaag cgggatcaag atatttggaa aattgatccg
    17041 ttcgatcatc tttactacgg atattgcgat catacgtaat acaaatgcga atgcagtaat
    17101 tgctgtgtat ccttttacac ctcatccagc gattactaaa agcattattg aggcagctga
    17161 cattccagtt ttctccggtg taggtggtgg tttgacacaa ggtgctcgtt cttcctacat
    17221 gagcatgttc gcagaagcgc aaggttcgtt aggggtcgtt ttgaacggtc ctacgccttt
    17281 agagacagta aaagaagttt gtcgagtagt tgatattcca gttatcagta cggtaacgtc
    17341 gaaatataca gaaatagatg aaaagttagc agctggtatt tcgattatca atatcagtgc
    17401 aggtaaagaa actgcagaag cagtacgcca ttttcgtaaa aaatatcctg aacttcctat
    17461 cattgcgaca ggtggtccaa cggaagaaag tattgctgag accattcaag caggagcaaa
    17521 tgccatcaca tatacgccac caagtagcgg agagcttttt agtcgtaaaa tggaaaaata
    17581 tcgggaaact gagaaaaaga atcatgaata acaggtgaga atcaaaagac gaggaagagc
    17641 agatagtggc tcttcctcgt cttttgtact ccagtactat ttgttcagaa ttcaaaaaaa
    17701 agagaaattt ttttgttttt ttacctgtaa tgtacataag aagagttaaa accttttgtt
    17761 attattagca ttctaacaaa aaaatgtctt gatttattga agcgctttct atagaatgaa
    17821 aaacgaataa aaactaaaaa aggcacccga caatttagta gcagctaaat tgtcggggct
    17881 tgaaaagtca gcgggaactg accaatcaag ttgccatagt caatgcttgt gcatcgacgt
    17941 gtatattgta cctaatttca tacagaatta ctagttattt ttctgttttt tagggaagag
    18001 cgcacacgaa gaagcatgct tatttagcta tgtcaaggat attgggcagt tgtctgatat
    18061 ccttttttct ttgcgtaatt atcctcccat cattgaagca agaagtgttt ttagcgatga
    18121 aagaagaatc agcactatct aatttttctt gaaagtattg agttgttcat cctaggcaga
    18181 acgataaggc aagtcttggt ttttattccc cttgaaaaag atcgtttaat aaatatgttc
    18241 tgtataaaaa tacagtatat tgaaaaataa caatggaagt agaggtgcag agaatgaaga
    18301 aaagaatgag aatcatctta gctttaagtg ttatgttatt cttcttgacg gcttgtgggc
    18361 aagaagatga caaaaaaatc acgctgaaca tggcttggtg gggttcgcaa gtccgacacg
    18421 atgcaacagt aaaagtaatc gagctttatg aagaaaaaaa tccccatgtg acgatcgatt
    18481 atgaatttta tgattatgaa ggatattaca caaaattagg tacactagca gcatcagatg
    18541 atatctggga cattttccaa ctgaacaatc aatttcctca atacatcaat cagattgaac
    18601 cattagatga atatgtggag caagggataa ttgatatttc taatgcagat gaatcctatt
    18661 tagcaacgac gacttacgac gggcaattag ttggactgtc taacggggtg aattcgtttg
    18721 cgattgctta caataaaaat atatttgaac aattaaatat ccctgaacca gcttcaaatt
    18781 ggacatggga tgaatttgaa caaatcagct atcaaatcac tgaagaatta ggaatctatg
    18841 ctatttcaag attcgaagat tttatagctg gctgtattat tcaaatacca caagttgaga
    18901 aaggattgaa tttttataat cgggaagatc tatctaacag cttgggattt gaaaatccag
    18961 actatctaac agattttttc cagctgcgta aaaatctggt agacaaaggg gcccatccag
    19021 aaccaggtgg tggagctgca agtacgacgg atctgtcttt tcaggcggtt agggatagag
    19081 aagcggcaat ggtgtttcta tcaagtaacc agttgacaga aatattgtca ggggctcctg
    19141 aagatatgga gattcagttg attaatccac ctcgcagaaa agcagatgga gaatctggta
    19201 tccctttacg ttcttcgcaa atgctgagta tggcaaaaag ctcagcccat aaagaagaag
    19261 ctgctaagtt tattgatttc tttcaaaaca gtgaagaagc caatgaaatt ttaaaaggag
    19321 aacgtggtgt accaatcatg tcgaatattc gagaacagct cgctcgtgca gatgatgcaa
    19381 ctttgcaagc gacatttgat tatttagaaa tgatttccga tatggacaac ggcgaagtga
    19441 atgtctttga tagtgctttg aatccggaaa ttcaagacca atacaatctt ttagttgaaa
    19501 aagtcattta tgaagaagta acagcagaag aagcagcaga gagtctttat gagtttgcgt
    19561 caaacattct aggatagcag caggaggaag gcatgaaaaa agaaaaacta aaacggttct
    19621 tggatcaaga caatgtggtc ggttacgtat ttgcttctcc attcatccta ggatttctat
    19681 tgataacagt gataccgatg atcttgtcac tttattattc gttcaccgat tatcgtttgg
    19741 gaaatccagt ctcatggatc ggcttggata attatataaa gatgctgagt gatgcccgtt
    19801 ttttgaactc catccgagtc acttttctct atgtatttgc atctgtacct gtgaaactga
    19861 tctttggtct attgattgct tatcttttaa cgaggaatat caaaggaacg actttctttc
    19921 gttctttgta ttatgtgcct agtttgatcg gtggcagtat ttctatcgct ctagtttggc
    19981 gggagttgtt ttctaaaaac gggttagtga atattttcct ggaacgatta ggcgcacctg
    20041 cgctttcctg gtttagcgat caaaaactgg tcatgatccc tcttgttttg atgtctgctt
    20101 ggcaattcgg ttcggcaatg atcatctttg ctgcaggtat caaagagatc ccaggctcgt
    20161 attatgaagc ggcagagatc gatggagcga acaaatggca gacgttttac aagatcacgt
    20221 tacctcttct ttcgccgatc attttgtata acttggttat gcagacaatc gcggcgttta
    20281 tgacttttac gcaggcattt atcattaccc aaggtggtcc gaatgatgcc acgaactttt
    20341 atgctcttta tgtgtataac caagcttttt cttttaattc tatggggtat gctagtgcta
    20401 tttcgtggat catgctgttg attatgggag ctgtcacctt tctgattttc aaatcatcaa
    20461 aaaaatgggt cgttgtgtga atgagaggag gaagaaatga ataatacagt aacgaaatgg
    20521 tttcaatacg gttttatttt gctattaggt ctagtcatga tttatccggt cttatggatg
    20581 atcgcaggtt cttttaaaac ggatcaagag attatgagtg gttcgctgaa tttgattcca
    20641 gagacttttc aatgggaaaa ttacgcaaaa ggatgggcgg ggtttgctgg aatttcattt
    20701 tttactttct ttaggaattc gtttgtcatc tctgcagttt cgacgatagg gacagttctg
    20761 agctcgactt gtattgctta cgcattatcg agaatcgatt tcaaaggaaa aagattctgg
    20821 ttcgtcgtca tgttagtaac gatgatgatt cctgcacaag tgattttgat tccgcagttt
    20881 attatttaca atcggctgaa tttggttggg acgtatgtgc cgctgatttt accacatttt
    20941 tttggacaag catttttcat ttaccagatc atgcaattca tggtcagcat ccccaaagag
    21001 ttagatgaat cagcgattat tgacggatgc agcaagtata gtgtctttac caaaattatt
    21061 tttcctttgc tgaaaccttc gatcatcacg acgatcatca tccagtttta ttggaaatgg
    21121 gacgacttca tgggaccatt gatttattta aacaagccga gatcttacac ggtatcgatt
    21181 gccatcaaat tatttgcaga cgctggatct agtacgactt acgcgagtat gtttgcgatg
    21241 tctacgttgt cgctgctacc ggtttttctg attttcttat tcttcaataa atatcttgtc
    21301 caggggatca gtacaagcgg tctgaagggc tgaatatgaa aaagccaata gaagagatgt
    21361 ttctttacaa gctcaactac tatgtctgtc ggattttttt aaccagtctt atcagtaatg
    21421 ctcttttcgc tttagttcta ggatgtttgt tcactttagg attagtgctg tctgctcgtg
    21481 ttgtctatct gatttatttt ttgatgatgt atccttctta ttatctatct gtccattact
    21541 tggcagaaaa aaaggtatgt tggaggaaag gaaaaatcaa acggatgtgt ttcatcatta
    21601 cgttgtttca gctgtttgtc attatttttg cagttgattg gtatttatta gtgggaaaga
    21661 ttggagcttg gtatctgatt ccttttctat tgtgtctgtt gctttttgta tcccatagtt
    21721 tttttagtac gctaattgat atctgcaaag aagataaagc agatagtctt gtttatttga
    21781 tcaaaaggaa tatctggctg ttgtcaaaaa acgtcaaggg gtttctgtgg tatttatgcc
    21841 ttttgtatgg actgttctgg tgtatacagc atgatttttt actcactgca ttactgtatc
    21901 cagggctgcc atttgtcaca gtattttgct taaaagaaaa ggagaatgac tttaaatgaa
    21961 gaaaaaatat gtacaagtcg gacttggtgg gagagcgcgt tttttttatg aggcaattgc
    22021 ctctacgtat catcaaactt gtgaactagt cggtttttgt gatgtatctc aaacaaggat
    22081 ggattatgca aatcggatac ttgtgaacaa gtatggacag aaaaaagtaa agacatgtat
    22141 gccatcacaa tttgagcaac tactggatga cgtacggccg gctgaagtca ttattacgtc
    22201 agtagatcga acgcatcatg attatatcat tcgtgcaatg aaaaaaggat atgatgttat
    22261 ttgcgaaaaa cctctgacta tcgatgagga aaaagctcaa gaaataataa cggtccaaaa
    22321 acagacggga agaagcctgc gagtcacttt taattatcgt tatgcaccgt atcacacgaa
    22381 gataaaggaa ctgatagaaa gtgatgtgat tggtgatgtg ttttctatcc acttcgaatg
    22441 gctgttaaat acgcaacacg gggcagatta tttcagaaga tggcatcgaa ataagcacaa
    22501 tagcggcggg cttcttgttc ataaatctac ccatcatttt gatctagtga atttttggtt
    22561 gaacagccgt ccccaaaagg tatttgcatt cggtggatta aacttttatg ggatgcataa
    22621 tgcggagaaa cgcggagtag aaatatttta cagtcgatgc tatggaagcg aactagctaa
    22681 agctgatcca tttgctatag acctgtcagc caatgaggaa ttgcgagaaa tgtatttaga
    22741 agcagaaaaa gacagtggat atattcgtga ccgcagtgta ttttccgatg atatctctat
    22801 tgaagataca ctaggcgtaa cggtcaaata tgaaactggt gcgattttga cgtattcgct
    22861 aaacgcgtat atgccgtggg aaggattcaa tgtttctttc aatggcagca aaggacggat
    22921 cgaaatgaaa gtgatcgaaa agtcttacat caatgcaggt ggagataaaa aggatgaagg
    22981 agcagccgtt cttcagtcca tccatgtcta tccgatgttc ggtgagccat atgaggtaat
    23041 cgtcgaacaa gaagcgggcg gacatggcgg aggagatccg cgtttattag atgatctatt
    23101 tggtgaagaa aaagaagaag atccgtggaa tcgggcagca actcacgtag atggtatttt
    23161 gtcgattttg acaggaatcg cagcaaatca ttctattgct tcaggaaaag cagtagtgat
    23221 tgacgagctt gtcagcttaa aagaataaga atagatttac ggaaattact tcccgtagtt
    23281 tataaaatct tgccttattg ccgataggct gtcttttgaa cactatttat tcggaaaaag
    23341 gggctatgac atgctttttg tcatgccttc tttttatgtg ggctgctttt ttagatagga
    23401 gaaaataaaa ataactattt aaacccgaac aaaaaggaat aatgcattaa aaatcattag
    23461 attttacaaa aaaataagta aaccacgttg tcagtttcag aaagtagtgt tagactgttt
    23521 tcaatatttg aaacaacatt ttttattcaa atatggcaaa ctgccttcac gtgcaaaaca
    23581 gttgcaagaa aatgaattta cactattatt agttttacat acctacttat atatcgtcat
    23641 aatatggatg acagtttcta gccagtaccg taaatgctgg actataagta aaagattggc
    23701 tatttcaccc ttcttttctt atgataaagt ggagggtgtt tttctttaac agaaacatag
    23761 taggtttttg cacaaaagcg gttatttcgg tattaataaa tgataaagga gcaaataaag
    23821 tgaaagaatt agttgaacgt attcaaaaag acggacgtat attaggcgag ggcgtattaa
    23881 aagtcgacag cttcgtcact caccaagtag accctgtatt gatggaacaa atgggaaaac
    23941 ggtttgcaga agtatttaaa gaacaaggaa tcacgaaagt tgtgaccatt gaagcttctg
    24001 ggatcgcacc agctttattt gctgcaagag aattagatgt tccaatgatc ttcgctagaa
    24061 aagcaaaaag tttgacgatg gatgaagaat tattgacagc ttctgtttat tcttttacga
    24121 aacaagtgac aagtacgatt tctatttctc gtaaattttt atcttcagaa gacaaagtat
    24181 tgattatcga cgatttcttg gcaaacggac aagcggcaaa aggattgatc gaattatgcc
    24241 agcaagctgg agcgcaagta gaaggaatcg ggatcgtcat cgaaaagtct ttccaagatg
    24301 gacgtcagtt acttgaagaa atgggattga gagtcgtttc tcttgcgcgt attgcttcct
    24361 tgacgaatgg acaagttgag tttcttgagg aggacgcata atcgtggaaa aagaaacaca
    24421 aaatggaaaa gctgcggttt tagggctgca gcatctgcta gcgatgtatg caggagcagt
    24481 agcggtgcct cttttgatcg gtacaggact aggctttaat caagaacaga tgacttattt
    24541 gatttcaatt gatatcttta tgtgtggatt agcgacatta ttgcaattga cagttaaccg
    24601 atttttcgga attggcttgc ctgtcgtttt aggatgtgcg atccaagcag tagctccact
    24661 gatcttgatc ggaaccaatg aaggagtagg agctatctac ggatctatta tcgcttctgg
    24721 gatctttgtt gtgttagttt ctggtgtctt ttctaaaatt aaaaaactat tccctcctat
    24781 agtaacagga acggttatca ccgttattgg tttgactttg attcctgtag caatcgagaa
    24841 aatgggtgga ggtaatgcaa cagcagctga ttttggtgac aaaacaaatc tgctacttgc
    24901 atttgtgacg attctcctaa ttgtaggcgt gcaaatgctg gcaaaagggt tcattcgttc
    24961 aatcgcggta ttgattggtt tagtcggagg aagcatctta gctgctttca tgggaatgat
    25021 tgatctgagc gcgattggag atgcgccgtt gttccatgtt ccacaaccct tttattttgg
    25081 taaaccaact ttcgacgtat ggtcgatttt actaatgatc atcatttcca tggtcagcat
    25141 ggtagaatca acaggtgtat attttgcact tggtgacatt actgggaaaa atgttggaga
    25201 agaagaattg aaaaagggat atcgtgcaga aggacttgcc gttatcttag gcggtatttt
    25261 caatacattc ccttatactg gcttttctca aaatgtcgga ttggttcaac tttcgggaat
    25321 caaaacaaga aaacccatct atttctctgc attcttcttg attgttttag gattattccc
    25381 taaaatcggt gcgttagcac aaatcatccc tgagccggta cttggtggag gaatgttagt
    25441 catgttcggg atggtggcag tccaaggaat ccgtatgctg ttggaagttg atttcagcaa
    25501 tgataaaaat ctattgattg ttgctgtttc tgttggattt gggctgggtt tcaatgttac
    25561 gccgacatta ttcagtcaaa tgccgcaaac attgcaaatg ttcactggaa atggcatcgt
    25621 aatgagtagc attacggcta tcatattgaa tttgatcttt aatggattga aaaaagatga
    25681 agaaatattg gaagaagcct tcaagaagta atagagcaaa agaaaggttg tgaaatcagc
    25741 gttttgacct gattgttaga gaaacaaacg gaaaaatagt tcctcgtatt ttgccgtttg
    25801 tttgagctaa taccgagggt cagctgattg aacaccgttg aaggaagagg tcgtgagcct
    25861 gccgttcatc tccgagtcac aaggaacaat ttcaaaaaaa agcttctcat gtttgttgaa
    25921 tattgttcct tgtgaccgag gagttggcag gagaacaccg tttatccata aaaaggaatg
    25981 tgacctaact tttgtcacat tccttttttt tatctttaat aaacacgaac aataaaatgt
    26041 ttttctttgt taacattcga aatttctttg acatcaaaaa aagtgattgt taaaatatga
    26101 atgtagaaaa actgggagga aaaacaatga cagcaaaaat ctctgtgatt atggggagta
    26161 cttcagattg ggaaacaatg aaagaagcat gtcaaatatt ggatgaattc ggtgtgtctt
    26221 atgaaaaaaa ggtcgtttct gctcatcgaa ccccagattt gatgtttgaa tatgcacaaa
    26281 acgctcgaaa aaaaggaatc aaaatcatta ttgcaggagc agggggagcc gcgcatctgc
    26341 cgggtatggt cgcatcaaaa acaactttgc cggttatcgg tgtaccggtc caatcacgaa
    26401 cattaaatgg tttggattcg cttctttcga ttgtccaaat gcctggagga gtacctgtag
    26461 ctactacagc aatcggaaaa gcgggagcaa ttaatgctgg actacttgcg atacagatgc
    26521 tttccatgta tgatttagaa ttggaggcta aattggctga aagacgttca atgttggcta
    26581 aaactgttat agaaagtagt gatcaacttg gttaaacctt tgttgcctgg ttcaacgatc
    26641 gggatcgtcg gtggcggtca attaggtcgt atgatggcga ttagtgcaaa agaaatgggc
    26701 tttaaagtag gtgtgctgga tcctgtgagc gattgtccag ccgcacaagt tgcagattgg
    26761 cacattgaaa gtgtctatga tgacaccttt gcgctgaaag agcttgccag aagaacagat
    26821 gtcattacct atgaattcga aaacgtcagt gtagaggcgt taaatgcgat tttacccatg
    26881 tcatttattc ctcaagggac agacttgctt gcgattacgc aagaccgcct gttagaaaaa
    26941 tcttttttgg aaacgaataa catcgtgatt gctccttatg caacgatcgt cagtccgacg
    27001 gatatccaag atgcgattga cgggatcggc tatccttgtg ttttgaaaac cactcgaggc
    27061 ggctatgatg gcaaggggca gtatgttttg aaaagtcggg cggatctagc tcctgccatg
    27121 gaccttttaa gagaaggaac ctgtgtttta gaagcatgga tcccgtttga aaaagagatt
    27181 tcgatcatgg tggcaggaaa tggtcaagga gatttcacga cttttccggt cgtggaaaac
    27241 atccaccaca acaatattct tcatgaaacg attgctccag ccgcgattga tcaggatgtg
    27301 atcgaggagg ctgagcgtat tgcccgtgtg attgcagaag cagtgagcct ttcaggtgtt
    27361 ttaggggtag aaatgttttt aacaaaaaca ggcgggctat atgtgaatga attagctcct
    27421 cgtcctcaca attctggaca ttattctatt gaagcttgtt ctatgagtca gttcgatgcc
    27481 catattcgag gtatttgtgg ttggccatta ggagaaaaag tacgtttgat aagcaatgca
    27541 gtaatggtca atgttttggg agaacaactg ctggaaagct atcgcttgat tgagaataag
    27601 ccagactggc aattccattt ctatggaaaa aaacaagcaa aaaaaggtcg caagatggga
    27661 catatcacta tcttgacaga agacatccag caaacgctgg aagaaatcaa acaaacgaat
    27721 atttgggatt agaggagaaa aaacatgtta gaacgttata cacgccctga aatggggaat
    27781 atctggacgg aagagaacaa ataccgtgcg tggctcgaag tcgagatttt agcagatgaa
    27841 gcttgggcag aacttggtga aatcccgcaa gaagatgtga aaaaaattcg tgagcatgca
    27901 tcatttgata tcgatcggat cttggaaatc gaaaaattaa cacgccatga tgtcgttgcg
    27961 tttacacgag cggtttctga aacattggga gaagaaagaa aatgggtcca ttatggtcta
    28021 acaagtacgg atgtcgtaga tactgcttat ggctatctca tcaagcaagc aaatgatatt
    28081 ttacgggaag acttgaaacg ttttacggaa atcattggcg aaaaagcaaa agaacacaaa
    28141 tatacagtga tgatgggtcg gacacacggt gtccatgctg aaccaactac ttttggattg
    28201 aaacttgcca catggtattc agaaatgaaa cggaacatcg aacgatttga acatgcggca
    28261 aaaggagtag aagcaggaaa aatctcagga gcagtgggaa cgttcgcaaa tatcccgcca
    28321 tttattgaag agtatgtatg taaacaattg gggatccgtc cacaagaaat ttctactcaa
    28381 gttctccctc gggatctaca tgctgaatat ttctcagcga tggcgcttat tgctacaagt
    28441 attgaacgtt ttgcaacaga gatacgcgga ttgcaaaaat ccgaaacacg tgaagtagaa
    28501 gaattttttg cgaaaggtca aaaaggttct tctgctatgc cgcacaaaag gaatccgatc
    28561 ggttcagaaa atatggctgg tctagccaga gtggtgcgcg gacacatggt cactgcctat
    28621 gaaaatgtca cattatggca tgaaagagat atttctcatt cttcagcaga acgaatcatc
    28681 ttgcctgata ccacgatttt attgaattac atgttaaatc gttttggcaa tatcgttaaa
    28741 aatctaacgg tctttcctga aaacatgaag cggaatatgt ctgcaacatt tggtttgatt
    28801 tatagccagc gcgttatgct gaaattgatc gataagggaa tgacaagaga agaagcttat
    28861 gatcttgttc agccaaaaac tgcgtacgct tgggacaacc aagtgcagtt ccgcccgctg
    28921 ttagaagaag atccaaccat cacatcggta ttatctaaag aagaaataga cgatgccttt
    28981 gattatcatc atcatttgaa aaacgttgac gtgatattca aacgagtagg tttggaataa
    29041 agaagagtga gttgcaaaat tcaattaaaa tagaatagca aaaataacca aaaaagaagc
    29101 agatccgtta gcggttctgc ttcttttagt ttattatatt ttcttatcaa taataggaac
    29161 ctgattttaa aagcggctac ctctttaccg tgcctcttta catacaaaaa atagaggaat
    29221 taagcgtaaa tggcttggct attataacta aaatgctggt tatggagatg ttgcagcaag
    29281 ttttcgagaa aagcgtattc catctcttgt ccattgaata agatctggat acgataatga
    29341 tttttgaaac gcttctgttc atgaatctga ctgattgaaa gttcgccttt gtgttctgtc
    29401 tgataaagaa aagtcctttt acggcattca gcacagcgct tggttgctcg gcggattttt
    29461 cggaatgtat aaaaactagt atctttattg cataaataaa cgtctttgat cgacagcaaa
    29521 tcattatata gacaggaacg ttcttcagct gttaattctg ataaaaaaag ctgaccttgt
    29581 tttattgcat cttccatcgg caagtgcctc ctcgaaccca ttatccaaat ttttagttaa
    29641 ttctaatata ccatcattaa gtataaatgt aaacgcttgt tgatggtata agattttcgt
    29701 ttctgataaa acacgaacat taaaagaatc taatgcctta aatgtacata tttttgttga
    29761 tttataaaaa gatgattgct aaaatttaaa gtgaaaaaac gaacaaaaaa cgatgtattt
    29821 gcttttaatg taaataaaaa ggagagacgt acatggagaa gaaagaactt ttgtatgaag
    29881 gaaaagccaa aaaaatatat cttacttctg atgagaactt gatttggata gaatatttag
    29941 atcaagcaac tgcgttgaat ggcgcgagga aagaccagat aagtggaaaa ggagaattga
    30001 ataatcaaat cacaagtgca attttccgtt atctgactca aagaggaata aaaaatcatt
    30061 ttattgaaca attgtctaaa accgaacaat tagcaaaaaa agtacaaatt atccctctgg
    30121 aagtggttat ccgaaatatt gcagcaggaa gtttctcaaa acgtctaggt gttcccgaag
    30181 gtaaagaatt catcaaacca atcattgaat tctactataa ggatgaccag ttggatgatc
    30241 catttatcaa tgaagagcat atctatttct tacaacttgc aaagccggaa gaaatcagtg
    30301 agctgaaagc ccaagcgttg gctattaatg aaattttgac cgaattattt gctaaggtcg
    30361 gtttggtttt agtagatttc aagctagaat ttggccggtc gaatggacaa attttgcttg
    30421 cggatgaagt aacaccggat acttgccgat tatgggatga acgtacgaat gaacatatgg
    30481 acaaagacgt ttaccgcaga gatctaggtg atttgatccc agtctatcaa gaagtatata
    30541 accgtctatc ggaactaact gaagaaaagg agcgagtatg atgtatttcg taaaagtata
    30601 tgtaacctat aaagattcag tgttagatcc tcaaggggag gcagtaaaaa aagcagtcca
    30661 tcgtatggga tatgaaacaa tcgaagatat ccgaatagga aaatactttg agatacaact
    30721 tgctgatgca gaagaaccaa tcgatacagt gattgaaacg atttgtgaca agctgcttgc
    30781 aaatgtaaat atggaaacat accgatatga aatcaaaaaa atggaggaag tataacatga
    30841 aatttgcggt catcgttttt ccaggttcaa attgtgacgc agatatgtta tgggcagttc
    30901 gcgagatcat gggcgctgat gccgaatttg tacggcatga tgagtcctct ttagaaggat
    30961 ttgatggggt tttactgccg ggaggattct cctatggtga ctatttgaga tgcggtgcga
    31021 ttgctcggtt ttctacaatc atggaagaag tgattcgttt tgctgaagaa gggaaaccag
    31081 tattcggtac atgcaatgga ttccagatat tgacagaagc aggacttttg ccaggtgtac
    31141 tgagacgaaa tgagtctttg cattttattt gcaaaactgt accattgaaa gtcaacccca
    31201 agacggcctt tacttcagag tatgaagccg acgaaataat ctatcttcca gttgctcatg
    31261 gggaaggaaa ttatttctgt gacgaagaaa cacttcaaga actaaaaaaa aataatcaga
    31321 ttgcctttac ttataatgac aacatcaacg gcagcgtgga aaacattgca gggattacga
    31381 acaaagcagg gaatgtgtta ggaatgatgc ctcatccaga acgtgcagta gaagacttgc
    31441 taggatcaga agatgggaaa cggttctttg cttccatttt aaaaaacttc ggaaaggtga
    31501 ccgtatagat gaaaacattg aaagaaccga cagctgaaga gatcaagaaa aaaagaatct
    31561 atgccgaatg gggattgact gatgaagaat atcgaatgat cgaagaagat atcttaggaa
    31621 gaatgcctaa ttatacggaa actggactgt tttctgttat gtggagcgaa cattgttcct
    31681 ataaaaattc taaaccggtt ctacgcaaat ttcctactag tggacctcat gtgttgcaag
    31741 gccctggaga aggcgcaggc atcgtcgaca tcggtgatgg actagcagtt gtcttcaaag
    31801 cagaaagtca caatcatcct tcagccgtgg agccttatga aggagctgcg acaggagttg
    31861 gcggaatcat cagagatatt ttcagcatgg gggctcgtcc aattgcgttg ctagattcat
    31921 tgaggttcgg cgaattagat aatccaagaa cgaaatattt actagaagaa gtagttgcag
    31981 gtatcggcgg atacggcaat tgtatcggca taccgactgt aggcggcgaa attgcatttg
    32041 atccttgtta tgaaggaaac ccattagtca atgcaatgtg cgtaggtttg attgaacaca
    32101 aacatatcca gaaagggcaa gccgctggcg tcggtaattc gatcatgtat gtaggtgcaa
    32161 aaacaggtcg cgacggtatc cacggagcaa cttttgcctc agaagaattt aatcaagaag
    32221 aagaacagca acgttctgct gtacaagtag gcgatccttt tatggaaaag ctgctgcttg
    32281 aagcgtgttt ggaattaatc ttgcatcatt cggattctct aataggtata caggatatgg
    32341 gagcagcagg acttgtttca tctagtgcgg aaatggcttc aaaagctggc tcaggactta
    32401 tattgacatt agatgaagtc ccacaaagag aaactgggat gacaccttat gaaatgatgt
    32461 tatcggaatc acaagaacgg atgttgattt gtgttaaatc aggagaggaa gaaaaaatcc
    32521 aagaactatt tcagaagtat gatctagatg cagtcactat cggtaaagta acagacgacg
    32581 ggcaatatcg gctatatcat caaggagaag aagtagcaaa tctgccagtc gatgcattag
    32641 ctgaagatgc accagtttat cataaagaaa tgaaagaacc agctcgaatc gtagaatttc
    32701 agcagttagc tccttatcag ccagttatcg aagaacctgg acaagtattg cttgatctgc
    32761 tgcagcaacc gacgattgct tcaaaacgtt caatctacga aacttatgat tcacaagtac
    32821 aaaccaatac agttgtccgt ccaggaagcg atgctgctgt gatgagggtt agagggacga
    32881 ataaagcgtt agctatgacc acagactgca acgcgcgcta tctgtatttg gatccaaaaa
    32941 tcggaggaca aatagcagtg gcagaagctg ctagaaatat tattgctagc ggtggtaagc
    33001 ccttggcgat caccgactgc ctgaactatg gttctcctga taaaccagaa gttttttggg
    33061 aattatcgac ttcagctgac ggaattgctg ctgcgtgtga aacgttagga actcctgtta
    33121 tttcaggaaa tgtctcttta tataatgaaa cggatggtca agcaatctat ccaacaccaa
    33181 tgattggtat ggtcggattg attgaggatc ataaacatat cactactcag gaattcaaaa
    33241 aatcagggga tctaatctat atattaggta agacgtttgc agattttgat ggcagtgaat
    33301 tacaaaagat gcagcttgga agaatcgaag gtgtgatccg aaactttgat ctatccattg
    33361 aaaagagaaa ccaagagtta gtattgacag ccatccaaaa cggattgatt gaaagtgctc
    33421 atgactgttc agaaggtggc ttggcaattg cactggcaga atcagctttc aaacatcagc
    33481 tagggatatc tgtacagttt gaattgtcct ctgctcaatt atttgcagaa actcaatcaa
    33541 gatttgtgtt gacagtcgca ccggaaaata agacacgatt cgaagaaatg atgggagatg
    33601 ctgctgtttt agcaggtaag gtgacggatg aagcgataat cgaaatttca gcaactgacg
    33661 gtcagatcaa aatagaaact gcagtagcaa gaaagtgctg ggaggatgca atcgtatgtc
    33721 ttatgaagtg aagagtttaa atgaagaatg cgggatcttc ggtgtttggg gtcatccaga
    33781 cgccgctagt gtggcatact tcgggctcca tagtcttcag catcgaggac aagaaggtgc
    33841 aggtatcgtg tccaatgatc gaggaaagct taaggggcat cgagatttgg ggctgctttc
    33901 tgaggtattc aaagatcaaa gaaaattgca gcagttaaca ggagaagctg caatcggaca
    33961 tgtacgttat gcaacagcag gcaatggaag tgtggacaat attcagcctt ttctattcaa
    34021 atttcatgat cagtcgatcg gcttagcgca taatggtaac ttgatcaatg ctcgttccct
    34081 tagaaaagag ttagaggaaa atggggcgat cttccattct aactccgata cggagatatt
    34141 gatgcatttg atccgacgaa gtgaagaaac agatttttta aataaattaa aaagtgcttt
    34201 gaatgaagtc aaaggtggct ttgcctattt attaatgaca gaaacggcaa tgattgccgc
    34261 tttggaccca aatggttttc gtccattagc aatcggacaa atgaaaaacg gagcatacgt
    34321 gattgcctcg gaaacttgtg cattagaagt tattggggca cgttttataa gagatgtcca
    34381 gcctggtgag atagtgatca tcaatgacga aggaatccag attgattgct ttacgaagga
    34441 cacgcagctt tctatttgtt cgatggaata tatctatttt gccagaccag attcaaacat
    34501 cgcagggatc aatgtgcata ctgcacggaa aaatatggga cggaatttag cgaaagaatc
    34561 gccagtaaaa gcagatatgg tcgttggcgt acctaattct tctttgtcag ccgcaagcgg
    34621 ttatgcggaa gctagtggaa tcccttatga aatcggatta gtaaaaaatc aatacattgc
    34681 ccgaacattt atccagccaa cgcctgagct aagagagcaa ggtgttcgaa tgaaattatc
    34741 tgctgtacga ggagtagtgg aaggtaaaaa ggtgattatg gtcgatgatt caattgttcg
    34801 gggtacgaca agcagacgga tcgtccatct tttgaaagaa gcaggggcaa aagaagtcca
    34861 tgtgaggatt gcttctcctc ctttgaaata tccatgtttt tacgggattg atattcaaac
    34921 gcgtaaagaa ctcattgcgg caaatcacac gattgatgaa atcaaagaat gtatcggtgc
    34981 agattcttta ggttttttaa gtgaagaggg tctgatcgaa tcgatcggtc tggaaaaaga
    35041 tgctccgtat tcaggattgt gtatggctta tttcaacgga gattatccga cgccgttata
    35101 tgactatgaa gaaaaatatc aggaatcact aaaagaaaaa gtatcgttct tttaaaagca
    35161 gaccaagaag ggagtatatg aattcaatgg caaatgcata cgcaaaagct ggcgtagatg
    35221 tcgaagctgg atatgaagta gtagaacgga tcaagaagca tgtcaaaaaa acggaacgtt
    35281 taggtatgat gggcgctata ggaggattcg gcggttgttt tgatttatcg gtctttcaat
    35341 taaaagagcc agtccttgtc tcggggaccg atggcgtggg gacgaaatta gcagtggcaa
    35401 tcgaagcaga caaacacgat acaatcggga tcgactgcgt agcgatgtgt gtcaatgata
    35461 tcttagcaca aggcgcagag ccgttatatt tcttagacta cttagcaacg gggaaaaatg
    35521 atccgttacg tttggaaaat gtagtaaaag gtgtagcaga tggatgtgtt caaggaaata
    35581 tggcgctgat tggcggagaa acagctgaaa tgcctggtat gtactcaggt aatgattatg
    35641 atctagccgg atttgctgta gggatagctg aaaaatctca attattgaca ggagaaacga
    35701 tcaacccagg agatgtgttg cttggaatag catctagcgg gattcattca aatgggtact
    35761 ctcttgttcg caaaattttc tttgagcagc agcagttttc tttatcatca aaactgccag
    35821 aattgaaaaa aacacttggc gaagaattgc ttgagccaac gaaaatctat gtttcttcct
    35881 tgcttccgct gatcaaagaa aagaaaataa aaggagctgc acatatcact ggtggagggt
    35941 ttgtagaaaa tatcccgcga atgttaccag aatctatggc tgcacaaatc aaacttggct
    36001 catggccgat tcttccaata ttcggctgtt tacaaaaata tggtgaaatt cctgagatgg
    36061 agatgtatga aatcttcaat atgggcatcg gtatggtcct ggcagtcgaa gaatcgaaag
    36121 taaaagaaat agtacacgaa attgagcaag ctggcgaaaa agtttatgaa atcgggaaag
    36181 tgatcgccaa agaaaaacat cgtgtggtgt ttataggaga agaacgatga gaatcgctgt
    36241 ttttgcttct ggaaacggaa gcaactttca agcgctagcc gattatctat cgaaaaaagg
    36301 attggaatct tcgattgact ggttgttttg tgatcagcct gaagcatatg ttttgaaacg
    36361 tgcaactgcg ttgagtgtac cggcagattg tttttcacct aaagaatttg attcaaaaaa
    36421 agagtatgaa gaagcaattt tgcataaatt gaaagaaaaa aagatcgatt tgatcgtatt
    36481 agctggatat atgcgaatca ttggaccggt attgctgaaa aattatgata aaagaatcat
    36541 caatatccat ccgtctcttt tacctgcctt ccctggtttg catggcatac gtgatgcctt
    36601 tgaagctgga gtaaaagaaa cgggtgtcac aattcattat atcgaccaag gtgtagatac
    36661 tggaccaatc atccggcagg aaaaagtgag aattgaacag gaggacacgt tcgattcttt
    36721 agaagaaaaa atccatagag tagagcatcg aatctatcca gaagtgatta gtgaaattat
    36781 tgaaaatgga ggctatctga aatgacaaga gcattgatca gtgtatcaga taaaactggg
    36841 attgtagcat tcgcccaagg tttgattgaa caaggagtag aaatcatttc aacaggagga
    36901 acaaagcatg cgttggataa agctgggatc aaaacgcttt cgatcgaaga agtgacaggg
    36961 tttcctgaaa tgatggatgg ccgtgtcaaa acacttcatc ctaaaatcca tggtggtctt
    37021 ttaggaagac gcgatctccc tagccatatg gaggcaatgg aaacggaaaa tatccaacca
    37081 atcgactttg tttgtgtcaa tttatatcct ttcaaagaaa cgattatgaa accagatgtc
    37141 gaaatagcag aagctattga aaacattgat atcggtggtc ctagtatgct ccgtagtgca
    37201 gcaaaaaacc atgaatttgt cacctctgtt gttgatccat ctgactatga agaagtactt
    37261 tcagaattac gtcagtatgg gcatacttca ctttctttca gacaaaaact agcggcaaaa
    37321 gtctttcgtc atactgcggc atatgatgca ctgatcgctc aatacttaac ggattgggtg
    37381 gaagaaaaag aaccggaaaa attgacactt acgtatgaac gaaaacaaac gcttcgttac
    37441 ggtgaaaata gccatcaagc cgctgccttt tatcagtccg ctcttctgga aaaatattcg
    37501 atcgcagcag caaaacaact ccatgggaaa gaactttctt ataataatat ccgagatgca
    37561 gatgctgcgc tacggatcat gaaggaatat gacgagccaa cggtagtcgc cttgaaacac
    37621 atgaatcctt gtggtattgg gacagcatct acgattttag aagcgtggga agcagcgtat
    37681 gaagcagatc ctgtttcaat ctttggtggg atcatcgttt tgaatcgaga agtagatctg
    37741 ccaacagcag aacagatgca taagctgttt ctagaaatca tcattgcccc ttcttataca
    37801 caagaagctt tggaattact gcaaaaaaag aaaaacatcc gattactgac agtagacttt
    37861 gcgcaggaag aaaatcaaaa tcgagaagaa acggtctcag tcttaggtgg acttcttgta
    37921 caagatcaag atctggcaat agaaaatgaa gaagagtgga aagtcgtgac aaaacgtaag
    37981 ccgactccag cagaagcaaa agcattagtg tttgcttgga gagctgtcaa acatgtgaaa
    38041 tctaatgcga tcgtactggc aaacgaacgt cagacagtag gaatcggagc cgggcagatg
    38101 aatcgagtcg gttcagtaaa aattgcgatt gaacaagcgc aagagaaaat ggaaggtgca
    38161 gttttagcta gcgatgctta ttttccaatg gatgattcgg ttgaatatgc agccaaacat
    38221 ggtataaaag caatcattca gccaggagga agcatcaaag accaagattc tattgatatg
    38281 gcgaataaat acgatctagc aatggtcttt actgatgttc ggcattttag acattagaga
    38341 ggagtcggat aaatgaacat tttggtgatc ggtgcaggtg gtcgtgaaca tgcaataagt
    38401 aaaaaacttt tagccagtcc gaaggtggat acggtgtatt gtgcgccagg aaatccgggg
    38461 atgacaaaag atggtatcaa ttgtgtcgct atcagtgagc gagatcattc agcgttaatc
    38521 cattttgtta agaaagaggc tattgattgg agtatcgttg gtcctgaagt tcctttgtta
    38581 aatgggatcg tcgatgattt tatggcggaa ggattgaaaa tcttcggtcc gacaaaagca
    38641 gcagctctga tcgaaggttc gaaaagtttt gctaaacaat taatggatac ttatcaaatc
    38701 ccgacagcta aatccagaag ttttgaatgt tacgaaaaag cagcagcgta tatcaaagaa
    38761 aatggtgcgc caatcgtagt gaaagcggac ggcttagcag ctgggaaagg tgtagtcgtg
    38821 gctaccacag tgaaggaagc gctggatgcg gcagaacaaa tgttgaacca gcatcgattt
    38881 ggggatagta gcaaaaaaat cgtcgtagaa gagtttttgg caggagaaga attctccctt
    38941 ttggcgtttg tccgtaatac caaggtgtat ccgatggtca tttcgcaaga tcataagcgg
    39001 gcatttgata acgataaagg gccaaataca ggcggaatgg gtgcttattc accagtaccg
    39061 caaatttcaa aaaagatgat tgaaaaagct gtggaagaag ttttaaaacc agcggctttg
    39121 ggaatgaaag aactaggacg ctctttcacg ggtgttttat atgcgggttt gattgctacg
    39181 aaggaaggac caaaagtgat cgaattcaat gcgcgtttcg gtgatcctga aacgcaagtc
    39241 gttctttctc ggttgaagag tgactttgcg caagtaatcg atgatttatt agagaatcgt
    39301 acagtagagt taaagtggca gcaagaagga tacaatgtag gcgttgtggt tgcagctgcc
    39361 ggttaccccg aagaatatga gacaggaatg gttctaccag attttctgga agaagagctt
    39421 tctgtttact atgctggagt ttcctcaaat aacggcaaac tagttggagc aggaggacgt
    39481 ctttatttag tagaagcata cggtgagacg atcaaagaag cacagaaaaa aatttacacc
    39541 gcgttaatgg ataaaacgac agatggaaca ttttatcgta cagatattgg agaaaaagct
    39601 gccaatagcg aactgatcgt gaagtaaatc agctgcttcc atttataaaa aatacaacaa
    39661 acataatcgg aagcgccgat tttagtgaag ccttttcata aagagtgtgg tacacttttg
    39721 ttgtgtatat aaataaggag gagttcagat gaaaaaaatt caattaggta cttctgatca
    39781 gaaagcagct gcagtgatct taggctgtat gcgtatcaac aatgcggaag atccagtaaa
    39841 agtgattgag acagcttatg aaaacggcat tgattttttt gaccatgcag atatttatgg
    39901 aaacggtgaa tgtgaagaaa tctttgcaga tgctctagca aaaacatcaa tcaaacgaga
    39961 agatcttttt attcaaacaa aatgtggaat cgttccaggt gtgatgtttg atttttctaa
    40021 aaaacatatc attgaatctg tagaaaaaag tttgacccgt ctgaaaatgg attatgtgga
    40081 tgctttactt cttcatcgtc cagatacatt ggtagaacca gaggaagtag ctgaagcgtt
    40141 tgatgaattg gaacggtcag gaaaagttcg ttattttggc gtcagcaatc aaaaaccaat
    40201 gcaaatcgaa ttgctgaaaa aatatgttcg tcaaccatta gtagctaatc agctgcaatt
    40261 tggcttgaaa catactggaa tgatcgatca agggattcat gtcaatatga cagatgaagc
    40321 aagtatcgat catgacggta gtgtacttga ttattcccga ttgcatgata tgacgatcca
    40381 agcatggtca ccataccagt atggattttt tgaaggtgta tttatcggga acgaaaaatt
    40441 ccctgaatta aaccaaacat tagaagaact agcagctaaa tatcaaacga caccaactgg
    40501 cttggcttct gcttggattt tacgccatcc agccaatatg caagtgatcg ccggtacgat
    40561 gaacgtaggg cgaatcaagg aaatcgctca agcttcagaa attaatatgt ctcgggaaga
    40621 ttggtacgaa ttgtaccgtg ctgctggaaa cattttacct taaagattgt cataagcagg
    40681 tcaaaagggc tataaattaa aaagacagaa taacttgaaa gctgttttca gcttcgagat
    40741 attctgtctt tttttattgc aaatggaaag agtatcattg tacggatttc gtttattagt
    40801 tatttttgtt tttaag
//
LOCUS       DAFEOM010000018        40141 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.018, whole genome shotgun sequence.
ACCESSION   DAFEOM010000018
VERSION     DAFEOM010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.018"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            63..1277
                     /locus_tag="LP524_001108"
     CDS             63..1277
                     /locus_tag="LP524_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBL2234176.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            2266..2475
                     /locus_tag="LP524_001109"
     CDS             2266..2475
                     /locus_tag="LP524_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HBL2234177.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            2587..4200
                     /locus_tag="LP524_001110"
     CDS             2587..4200
                     /locus_tag="LP524_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL2234178.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            4235..5446
                     /locus_tag="LP524_001111"
                     /pseudo
     CDS             4235..5446
                     /locus_tag="LP524_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            5469..6395
                     /gene="glsA"
                     /locus_tag="LP524_001112"
     CDS             5469..6395
                     /gene="glsA"
                     /locus_tag="LP524_001112"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBL2234179.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            6603..8063
                     /locus_tag="LP524_001113"
     CDS             6603..8063
                     /locus_tag="LP524_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234180.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            8284..10047
                     /locus_tag="LP524_001114"
     CDS             8284..10047
                     /locus_tag="LP524_001114"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBL2234181.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            10131..10589
                     /locus_tag="LP524_001115"
     CDS             10131..10589
                     /locus_tag="LP524_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBL2234182.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            10698..11381
                     /locus_tag="LP524_001116"
     CDS             10698..11381
                     /locus_tag="LP524_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBL2234183.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            11528..12142
                     /locus_tag="LP524_001117"
     CDS             11528..12142
                     /locus_tag="LP524_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBL2234184.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            12158..12364
                     /locus_tag="LP524_001118"
     CDS             12158..12364
                     /locus_tag="LP524_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234185.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            12905..14035
                     /locus_tag="LP524_001119"
     CDS             12905..14035
                     /locus_tag="LP524_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBL2234186.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            14127..14882
                     /locus_tag="LP524_001120"
     CDS             14127..14882
                     /locus_tag="LP524_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBL2234187.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            14940..16979
                     /locus_tag="LP524_001121"
     CDS             14940..16979
                     /locus_tag="LP524_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HBL2234188.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(17088..18239)
                     /locus_tag="LP524_001122"
     CDS             complement(17088..18239)
                     /locus_tag="LP524_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBL2234189.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(18393..18923)
                     /locus_tag="LP524_001123"
     CDS             complement(18393..18923)
                     /locus_tag="LP524_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBL2234190.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(19022..19372)
                     /locus_tag="LP524_001124"
     CDS             complement(19022..19372)
                     /locus_tag="LP524_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HBL2234191.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(19529..21058)
                     /gene="ahpF"
                     /locus_tag="LP524_001125"
     CDS             complement(19529..21058)
                     /gene="ahpF"
                     /locus_tag="LP524_001125"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBL2234192.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(21068..21631)
                     /gene="ahpC"
                     /locus_tag="LP524_001126"
     CDS             complement(21068..21631)
                     /gene="ahpC"
                     /locus_tag="LP524_001126"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBL2234193.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(21775..22437)
                     /locus_tag="LP524_001127"
     CDS             complement(21775..22437)
                     /locus_tag="LP524_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL2234194.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            22621..24267
                     /locus_tag="LP524_001128"
     CDS             22621..24267
                     /locus_tag="LP524_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBL2234195.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            24258..25340
                     /gene="uxuA"
                     /locus_tag="LP524_001129"
     CDS             24258..25340
                     /gene="uxuA"
                     /locus_tag="LP524_001129"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBL2234196.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(25448..27001)
                     /locus_tag="LP524_001130"
                     /pseudo
     CDS             complement(25448..27001)
                     /locus_tag="LP524_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            27149..28290
                     /locus_tag="LP524_001131"
                     /pseudo
     CDS             27149..28290
                     /locus_tag="LP524_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(28416..29495)
                     /locus_tag="LP524_001132"
     CDS             complement(28416..29495)
                     /locus_tag="LP524_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL2234197.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(29598..30038)
                     /locus_tag="LP524_001133"
     CDS             complement(29598..30038)
                     /locus_tag="LP524_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL2234198.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(30095..30505)
                     /locus_tag="LP524_001134"
     CDS             complement(30095..30505)
                     /locus_tag="LP524_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBL2234199.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(30685..31119)
                     /locus_tag="LP524_001135"
     CDS             complement(30685..31119)
                     /locus_tag="LP524_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL2234200.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(31219..32067)
                     /locus_tag="LP524_001136"
     CDS             complement(31219..32067)
                     /locus_tag="LP524_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL2234201.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            32456..34222
                     /locus_tag="LP524_001137"
     CDS             32456..34222
                     /locus_tag="LP524_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBL2234202.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            34324..35583
                     /locus_tag="LP524_001138"
     CDS             34324..35583
                     /locus_tag="LP524_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBL2234203.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            35730..37031
                     /locus_tag="LP524_001139"
     CDS             35730..37031
                     /locus_tag="LP524_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL2234204.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            37034..37885
                     /locus_tag="LP524_001140"
     CDS             37034..37885
                     /locus_tag="LP524_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL2234205.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            37898..38713
                     /locus_tag="LP524_001141"
     CDS             37898..38713
                     /locus_tag="LP524_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HBL2234206.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            38864..40030
                     /locus_tag="LP524_001142"
     CDS             38864..40030
                     /locus_tag="LP524_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL2234207.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT        13597 a         7003 c         7805 g        11736 t
ORIGIN      
        1 tttttaatga caggcaatta tcaattatgg tagttgcctt ttttgattgg aggaattgaa
       61 gaatgaatga taaaaacttt attgaagaac taagacaaaa gcgtgaggaa tacggagtaa
      121 cacaaacaag gcttgctgtt gcctgtggta tcagccgtga atattacaac cgcattgaaa
      181 aaggaaaaca gccattgaat gacgaattaa gagaaatcat agaaaaacag attgagcgtt
      241 tcaatccgca agaaccacta ttcttattga ttgattactt tcgtgtccgc tttcctacta
      301 cggacgcatt ggcgattatc cgtgatgtat tacaactgaa atctaattac atgctttatg
      361 aagattatgg aaaatacgga tatgaaagca aatatgtact tggcgacatc aatatcatgt
      421 gttccatgca ggagcattta ggtgttttat tggaactgaa aggcaaaggc tgtcggcaaa
      481 tggaatgtta tctgctggca caggaacgct catggtatga cttcatgctg gattgtatga
      541 cggctggtgg tgtgatgaaa cggcttgatt tggctatcaa tgataaagca ggaatattgg
      601 atattccaaa gttaaaggaa aaatacaagg ctggcgaatg tatgtcctac ttccgtatgc
      661 agaaagatta cagcggtaca gaaaaatgcg gtagcgattt accaaagaat acaggagaaa
      721 ccttatatct cggttcaaca agtagcgaat tatatatgtg tgcttatcag aaaaattatg
      781 agcagtatgt caagaatggc acagaaattg aagatacgga gattaagaac cgttttgaaa
      841 tacgcatgaa gaacgaacga gcctattatg cggttgtaga tttactgacc tatcgggacg
      901 ctgaacgcac cgctttttct atcatcaatc attatgtccg ctttgttgac agagaggacg
      961 acaagccaaa aagtcaatgg ataacaaatg atgattgggc atggtttgta ggggaaaaca
     1021 gagagccgat acgcttaacc acaaagccag agccttacac tttacaaaaa gcgttgcatt
     1081 ggctacaaag acaagtcgca ccaaccataa aaatggtaca ggcattagac agagaaaatc
     1141 gtacaacgat actgaaagat atgattgagc aggcagaact aaaagacaag cataaacatt
     1201 tattacaatt agagaaatca accatagaag aacgtataga taccgctgtt ccacaagaga
     1261 atgacggtat tttttaatgt aattgaaaaa tttcagattt agtcagcaaa gagctgtttt
     1321 cattccctat gtgaagtgaa agaagaaaaa taaatttttt attaaatctg caacaaaatg
     1381 gctacttgcg tacagatatg gtgcgagaaa aggaaatcta aacttttttt gaaaataggt
     1441 cattaaatcg gggcttgctg tccctatatg aagtgaaaga agaaaaaagt tttgaaaaaa
     1501 tgacggaaat tcgtgattag tggacagtct tctgtgaaaa gaggaaaaac atcatcattt
     1561 tgtattcaaa caggcattag cagttgagtg ttgatgtgaa aagatgaaaa tactttgttc
     1621 tcaacttaac aatttaaggt ttccgttccc tatatggtgc aaagaaaata tttcattcta
     1681 ttgttggcag taacggtatg gtttgggtag ttagggtgtg aaaaggaaaa tattcttttt
     1741 tcatcatcaa cttagcaaaa tgacattttc tatccctata tggtgcatga aaagaagtta
     1801 aaatttttcc atttaaactt agcaaatgga cgtttgctgt acagatagta gtgaaaaagg
     1861 agaaaatcaa ccgttgattg ccaaaatgaa ccgttccatt tgtactaata atggaaaaag
     1921 ttttggattt tggttacgtt tctcctcttt tccaacgcga tatataggaa agacgatttt
     1981 tttctttcaa tcgttctttg acaactgaat aaagctattc aatacgtttg acagacagct
     2041 agccgttgaa acaaatacgc cacgaccttt cccctgcaag agcgagcgaa aacctatttg
     2101 tgttcttgta aaagatttgt cctcttttat cgccatgacc tgtactgtca gataatgata
     2161 ctcccgtaca gccacagctt gagcattaag agcgtcgcac gcaatgggta cggctacata
     2221 agaactatgc agaggtggaa ctcctgtggg ctatgacaaa agccgttgaa ttgcttagaa
     2281 agattttaca gaaagggggt atgtgttatt gataatgctg taaaaacaat tcaaaagaaa
     2341 aatggcaagt gtggcattgg caacagagga aagacaaaga ttgtagtaaa agagcatttt
     2401 tccgaaaagg gcaaaacaat ggaagaactt ctaactgatg tcatgctgga aaaagcaaag
     2461 cagacaatcg cataaaatcg gtgcagttgt aagaaataga ttgaatgaca aaaagtaccc
     2521 gtgttatact ttacttgcaa gcaggtattg taaacacggg ttagttataa aggaggataa
     2581 ctgacaatga aacaacagat ttacaatact gcactttatc ttaggttaag ccgagatgat
     2641 gaattacagg gcgaaagttc cagcattacc acacagagaa gtatgttgcg tttatatgca
     2701 aaagaacatc atttgaatgt cattgatgaa tatattgatg acggctggtc gggaacaaat
     2761 tttgacagac cgagttttca aagaatgatt gaggatatag aggcaggaaa aatcaactgt
     2821 gttgtaacga aagacctttc ccgtcttggc agaaattata ttatgacagg acaatataca
     2881 gaattgtatt tccctagtca taatgtccgc tacatagcga ttgatgacgg tgtagacagc
     2941 gaaaaaggcg aaagtgagat tgcaccattt aagaacatca tcaatgaatg ggtggcaaga
     3001 gatacaagcc gtaaagtcaa atcagcattt aagacaaagt ttgcggaggg ggctcattac
     3061 ggtgcttatg ctccgttagg atataagaaa caccctgaca tcaaaggaaa actgttggtt
     3121 gatgatgaaa caaaatggat tattgaaaaa atcttttccc tagcctatca aggttacgga
     3181 agtgcaaaaa tcacaaagca gttaagagca gaaaaagttc cgacagcgtc atggctaaat
     3241 tttacaagat acggtacttt tgcacatatc tttgagggaa aacccgaaag taagcgttat
     3301 gagtggacga ttgctcatgt caaggcgata ttgaaaagtg aggtttatat cggaaacagt
     3361 gttcacaata tgcagtctac ggtatcgttc aagagcaaaa agaaagtgcg taagcccgaa
     3421 agtgaatggt ttcgagtaga aaacactcac gagccgatta ttgacaagga agtgttctat
     3481 cgtgtgcagg agcagataaa atcaagacgc agacagacaa aggaaaaggc aacgccgatt
     3541 tttgcagggc ttgtcaagtg tgcggattgt ggttggtcta tgagatttgg aacgaataag
     3601 acaaataaaa cgccttacag ttattatgct tgcagttact acgggcagtt tggcaaaggt
     3661 aattgttcta tgcactacat tcgttatgat gtgctgtatc aagccgtatt ggaacgcttg
     3721 cagtattggg ctaaggcagt acagcaggac gaagaaaagg tattgaacaa gatacagaaa
     3781 gctggcaatg cagagagaat acgggaaaag aagaaaaagg caagtacatt gaagaaagcc
     3841 gagaacagac aaaatgagat tgaccgttta tttgcgaaaa tgtatgaaga tagagcctgt
     3901 gagaagataa cagagcgaaa ttttgtgatg ttgtccagca agtaccaaaa agaacagata
     3961 gaactggaac agcagataac aagcctaaga gaagaactaa gtaaaatgga acaggatatg
     4021 ataggtgctg aaaagtggat tgagttaatc aaggaatatt ccgtaccaaa ggaactgaca
     4081 gcaccattat taaatgccat gatagaaaaa atcctcatac atgaagcaac aacgaatgag
     4141 gataacgaaa gaatacagga aattgagata tattaccgat ttattggaag agttgagtaa
     4201 tcaacaagag taatattttt aactaaggga aaccggcggt gtctatactt gggtcagtcg
     4261 cacattggga aaacgattcg gttttgccgc aatctttttc caatggtttg agataacagt
     4321 tggctacttg accatgctgt atttcttaac tggtgcatta tcctatgcaa cgggtattgg
     4381 tgctatacaa aacaataaat atctgaaatt ggcaatccta ttgattattt tctgggctat
     4441 tttactctca cagttaaaag gaaccaaata cacttcattg attgctcgga tcggttttgt
     4501 agctggtatc ttgttgccag cattagtctt gtttggctta gggatccatt atgtcgcttc
     4561 aggcgctcca ctacaaacga tactgtctat gaaaacattg attcctgatt tttcgaaatt
     4621 gcctacgctg gttgtttttg tttcctttat tttagcatat atgggcgtag aaacatctgc
     4681 cagtcatgca aatgaaatgg aaaaccctaa aaaggattat ccattagcaa tgtttgtatt
     4741 ggttattctg gcaatcattt tagatacttt tggcggattg accgtcgcta ctacagtacc
     4801 tcaacatggt ttgtctctta atacaggggt gattcaatcg ttagaattcc tattaagaca
     4861 cattgatcca aatcttgcat ggattgcgaa aattcttgcg attcttgttt gtttaggtgt
     4921 catcggagaa attgcttctt gggtaacctc tccttcaaaa gcacttcacg tagcagcaga
     4981 agaaggatta cttcctgaat attttgcaaa agaaaacaca cacggtgttc ctgctcattt
     5041 gatgatcgca aatgggatcg ttgcaacaat ctgggcagcc gtctttacat taagcggcgg
     5101 ggggaataat ctttcatttc ttgcagcgat gtcattgact gttgtaatct acctgatgtt
     5161 gtactttatg tttttcgcgg gctatttgaa gctggttttt aaagatgaca gtcttgaaag
     5221 agcctatcag gttccaggcg gaaaagttgt aaaagtaatc gttgcttgtt taggtctttt
     5281 gacttcactt tttgcttttt gtatctcatt tgttccacct gctagtatcg cagctggaca
     5341 gcatgggatt tatgaaacga ttctgattgt ttcttatctt atcgtcttag ttattccatt
     5401 tacgatctac gcatgccgtc ataaattcca gcatgcagaa aaataaaaaa tgaaagctgg
     5461 ttattcaaat ggaagtaaaa gaattagaac aattgattgg aaaaaatttc cactattatc
     5521 aaacaggaca agtggcggat tacattccag cattagctga agtggatccc aaacaattag
     5581 gtatggcgat ttatgatttg gatacaaaac aactgattga gggaggagac gccgatgtgc
     5641 gctttgccat cgaaagcatg tcaaaagttc ccgtattgtt attagcaatc aaagacaacg
     5701 gaatagaaaa agtctttcaa acaatcaaca cggaaccaac cggttttgcc ttcaattcta
     5761 tcatgaatat ggaaatcaat catcgcaaac atccaatgaa tccttttgtt aatgcaggag
     5821 cgattgcaac aacctcgttg attgccggta aagatgcaga tgaaaaattt gagcgaatct
     5881 tgtcatttat gaaagagatc tgtgatgatc ctaatatcac attgaatgaa gaaatctatc
     5941 attctgaatc aagaacaggt gatatcaatc gctctcttgc ttactatatg aaaggtaatc
     6001 aaatgatcga aggagacgta ccggagatac tcgatgctta tttcagacaa tgctccctca
     6061 atgtcaccgc tacaggcata gcaaaattag ctgctgtgct ggcaaataaa ggaattgctc
     6121 cttggaatgg caaacaaatc attactgaag aaagtgctac aatcgtcaaa tcaattatga
     6181 caactgctgg tttgtatgat gaatcaggag agttttccgt ccatgttggt gtacctgcaa
     6241 aaagcggtgt aggcggcggg ttgatggcag ctgtgccaaa tcgttacggc ataggtgtct
     6301 tcagcccggc actagatcca tttggtaaca gcgcagctgg tatccaattg ttgaaagatg
     6361 tagtcaaaga actagacgct gacatctttg aataaccatt cttgaaagag gctggggtaa
     6421 aagtccaaac ggctcaaagc tacctacaat gaggctgaaa ttgtgagctt gtttgacttt
     6481 tgtcccagtt tcttttttta ttggtttttg aaagggcttt tctaaataaa aaggaaacaa
     6541 aaatacgatt tacagatatg attaaataaa atcataagaa aaactggagg cgtgtatcag
     6601 gaatgctgga aaattacatc gagcgaaata tatttagaaa agtgtatctg tgtgaacaat
     6661 tatttgaatt ccaagaaata gacatcgaac aaacagctat ttctctaagg gtgactacac
     6721 caacgatcct tcacgatcta gaaagtctag ccgagtgtct agaatattgt ataaaagaac
     6781 aagtaagaga gaagcataag tataaactcg tattcaaaca tggtattgct ctttctgagc
     6841 tcacacagtt tctgtacggt caatcttatt ttttaaagtt tttaagttat tattttaatg
     6901 gtatattcac gagtacagaa ctggctgatc ttgaatttat ttctttgagt aaagtatata
     6961 caatcaaaaa aatcgtattg gattttttta aagaaaatga ttatttgaaa aataaagaaa
     7021 tcattatccc agaatttgac tcgcggaaca tcttgctagc tttagtacgt tatgtcaatt
     7081 gggaaggcta tgaaaataaa aatccgtatg tagaagaagc ttgtaacgag ttgattgatt
     7141 atgtagagac tcattttttt aaaagaagat attcggaaga tgaaaagtcc tttatcatac
     7201 gagaaataga gatcgcaatc ggaagaaagg catttcccct tggactgaaa gaagaagata
     7261 agaaaagcgc agaaacgaag ccgttatttc aattagtctg tcaaggattg gaagagcaaa
     7321 aaggaattct agacttacaa gaagaagatg tctattatat tttttctttg ttcaacagta
     7381 gaaattatac caatgagaac atggagctgt taagaaaaga tttcgaagtt gtttataaaa
     7441 attttgttct caatgaccta tcactgaatg aattgatttt acagattatt agtatcgctg
     7501 aaatcgacga tgcagaaaat tttattttca agcaatcctt tttacaattt gtccgtacgt
     7561 tgtgggcaga tggacaagta tttttaccgg aaaggatcta tcttctatca gaaaaagaac
     7621 aacttctgta cggaaaaatt gtagaaatat tggaagagtg gaaaaagaaa aatcaaatcc
     7681 aactaagatg gaatgaaaat ttcattcgaa aattcgtcaa aagtctttct ttgatcaaca
     7741 gtgaacaagc ggggagacag gaaatagaaa tttttgttgt ttcagaaagt tcagtcaaac
     7801 aattttttta tcgaacccaa tttcatttgt atgcggaaga aggcatcgct gaaataaatc
     7861 ctattttatt tcgttcatta gaagaattgc ctgatgaatg tctatgtgcg aaaaaaagag
     7921 tggttttgtg cgatgctgct agctatcaaa atggattaga aactgaaaag acaaagatct
     7981 atccaatctc attaaaaggt gcaaacatcc atttgtatca tattgctcag gaacttcacc
     8041 tattaaaaaa acatatttct tgatgagacc ttaattagga gaccttctac aaagcttttt
     8101 aattgtctgt agaaggtttt tatttgagtc ctgattcgtg cgaagatcta cttatataac
     8161 aagctttctc tgaaattttt ttaaaatgct attgacttag agtcaactct aagtagtaga
     8221 atagataaag tgtaacaaat ctattttcat taaaagaaga tagtacggag gagagaatgt
     8281 attatgagta cagtattaca tgtattggat gcaatcaata gccccgcaga tgtcaaaaaa
     8341 ttatctgttg aggaaatgaa acagttggca gaagagatgc gccaaaaaat cttgcaaaag
     8401 gacagcgatg taggtgggca cgttggtccg aatctaggtg tagtagaatt aacgattgcg
     8461 tttcattatg tatttgattc accaaaggat aaaatcgttt gggatgtttc ccatcagtct
     8521 tatggtcata agcttttaac tggtcgaaaa aatggttttg aaaaaggaca ttaccgtgaa
     8581 gtgagcggat attccaacca acatgaaagt aaacatgact attttacagt ggggcacaca
     8641 tctacatcta tcagcttagc aacaggttta gctaaagcga gagatctgaa gaaagaatca
     8701 ggaaatgtgg tagcatttat cggtgacggc tccctaagtg gtggcttagc ttttgaagga
     8761 ttgaacaatg gtggtgtttt acaatccaat ctaatcgtat tagtaaacga taatgagatg
     8821 tctattgatg agaatcatgg cagtttatat aaaaacttag ctgagcttcg tcaaaccaac
     8881 ggtaaaagca gcaataacct atttaaagca atgggatttg attatcgtta cgtggataat
     8941 ggaaacgatc tggaaacggt gatccgttta tttgaagaag taaaagatat tgatcatccg
     9001 attgtacttc acgtccatac ggaaaaaggt cgcggttatc agccggcaat cgataataaa
     9061 atgaaatatc attggcatgt accatttgat ctgtccaccg gggaggcaaa gcaagtaact
     9121 tctggagaaa catttaatat gatcatcatg gattatttgg ataagaaaat gcaaacaagt
     9181 acagctccta ttgccgcagt caatgcagcg ataccaggcg cctttggact gaaaacattc
     9241 caagaaaaac atccagatca ttattttgac gtggggatcg ctgaacagca ctctatcact
     9301 ttcgcttctg ctttggcttc tcagggagta cgtccagtcg tcttccaatc aagtactttc
     9361 ttgcaacggg catatgatca attatcacat gatttagcga tcaacgagaa tccagtgatt
     9421 attttagtcg gtggcggaac gatctcagct agtgaccgaa cacaccaagg aacatttgac
     9481 attccttact tatcaagtat cccgaacatc aaatatcttg ctccagcaac aaaagaggat
     9541 atgatctcta tgctagattg ggcgttcgaa caaacagcgc agcctgtagc aatccgaatt
     9601 cctacacatg gtgtagaaca tggtacagct atcgcaaatg attatagtat tccagcctat
     9661 caaacagtaa ataatggagg aaaagtagca attttagctt taggcggttt tcttgaatta
     9721 ggaaaagaaa cagtaaaagc ttttagaaaa aaaggaatcg aagcaacgtt gatcaatcca
     9781 ggaacgatca cagaaaatga cgtagaaaca ttagataaat taaaagaaac acacgaatta
     9841 gtcatcacat tagaagatgg tagtttgtca ggaggatttg gcgaaaatat cagtcgttat
     9901 tatggtgatt cttcaatgaa cgtattaaat tttggtgcag agaaagaatt cacggatagc
     9961 gttcctgtag ggcagcttta tgaacgttat catttaacac caaaacaaat cgttgaagat
    10021 agtctgcgtg tcttgaatat tcaataattc attatgctca ccatccagat aattgtttat
    10081 actagtatct ggatggtttt ttcattataa aaagcaaagg ggtggatgtt atgtatcagc
    10141 aagtagaaga atatgtcaaa aagttccaag aaaaatatcc tagttatcaa tcagaaaaat
    10201 taggtttctt ttcatttggg gatacagaaa aaatggcaga cgagttgagc gaactagttg
    10261 taaaaaagat aaagactggt acatcaagtg gttacgatct gtataaacaa gagaatgagc
    10321 ctttaccaaa agttggtcaa ttagacgtgg tcctgaatgg aaaaaatgaa ccagtttgta
    10381 tcatcaaaaa tataaatgtg accatcttgc cttttagtga agtcacgaaa gagcatgctt
    10441 ggaaagaagg agaaggggat cggacattag cttattggcg gaaagtccat gctgagtttt
    10501 ttaaacatgc ttataaagaa gcgttgaaca tagaatttac agaagagaag ctggtcgtat
    10561 acgaagaatt tgaagtaatt ttcttttagt aagaaaggtc tgctgaaaga aatagctcat
    10621 tattgtctcc tgaatagaaa agggttacat ttttatatga agctgtattt ttatataaca
    10681 ttactggagg agatattgtg gatacactgg aacaaatcaa aggcggagtc gtcgtttctt
    10741 gtcaagcttt ggaaaacgag ccgctacaca gttcttttat tatggggaga atggctttgg
    10801 ctgcaaaaga aggcggagca gttggtattc gagcaaatac gacagaagat atcaatgaaa
    10861 tcaagcgaat cgttgattta ccggtaatag ggattatcaa aagagattac gatgggtcag
    10921 aagtgtttat tactgcgaca gaaaaagaag tggaagaact tttggagacg caagcagaaa
    10981 tcattgcact ggatgcaact tctcgtaaac gtccgacaga aaaaacaact gctgaacttg
    11041 tccaaatgat tcacgaacac ggtcggttag ctatggcaga tatttcgact tttgaggaag
    11101 cgcttcaagc gcaagaagat ggttttgatc ttttgtctac gactttagca ggctatacag
    11161 aatattcacg aaagacgcag acacctgatt ttgatttatt gaatcaaatc atagaacaag
    11221 tcgatatccc agtaattatg gaaggacata ctgactctcc tgaacaagta gagaaagcat
    11281 atgaattagg tgctttttca gtcgtagttg gatctattat cacccgtccg caattgatca
    11341 ctaaaagata tgtcgaagcc gcaagtaaag caaataacta aaaaacacgc acggcacaga
    11401 ttcttgacaa aaaagaaaaa ttgatagata gtttaaagca gattctaaga agatgaaaaa
    11461 agcattactt ttcattttgt cttcaagatc tgctttttaa cgaattttat agaaagaagg
    11521 cgaaaccatg tttgaacgga gtccttcaga attgacagaa cgtgaaaatt acaagttgct
    11581 gattggttca gtcatcccta gaccagtagc agtagtaacg actaaatcag caaaagatat
    11641 agtgaatatc gcacccttca gttacttcaa tatcgtcagt tcgaatcgac ccatcttatc
    11701 cttggcgatc cagagaaaac aagatgcagt aaaagacaca gcaaagaata ttcttgagac
    11761 aagagaagct gttatccata tccttgatga ggacaacgta gaagaagtaa ataaaacagc
    11821 agccaatctg cctaatgagg aaagcgaact gtacctgact gacttgactt taactgattc
    11881 agtaatcatt tcagtaccag cattgacaga agcaaaagtt cgttttgaaa catccttata
    11941 tgaacatgtc gagatacgga ataaaaataa agtaacagct gatttatttt tgctagaagt
    12001 acagaaatat catttaaatg aaacagtcta tgatgataag acaggaagga tcgatccaag
    12061 aaaattgaat gctgtaagcc gtttggcagg aaatgattat gcagcaatag gagaaatttt
    12121 cacgattgca cgtcccgaat aaaaaggagc actagttatg ttaaaaataa gctataaacc
    12181 atcgacagat tcaaaagaaa tgaaaaaaga atatgaaaca gtgaatgatt ttctgcaagg
    12241 gcagtactta gaagttccgc cattgcaaga ccattttgtc gttactactg ttactttaga
    12301 tggtaaggaa atagaaatgc cagatcaaac gattagcgga ttgtttaatt actttaacaa
    12361 ataaatattt attccgaaaa gtgaaaaaac atataaaaaa acacataaaa ctaaaaaatt
    12421 atcaaagaat cctggtgcaa ctgaaagaat ttgcaacggg attctttcaa cttatttgtt
    12481 atttttttct aaaaaatatt aaagattgtt aaatttcact atttcatttc aaattccatt
    12541 gcctaatcat taggaaaagt ctatcatgaa ctcgtaaata gaatatagta ataaccttgt
    12601 taggtgaggc tcctatacaa acataggctg ttgcccagaa acgtcgaaag tcgccaatgg
    12661 gtaaaacaga aactgttgga ttaaggcttt ttccaatagc taagaatgtt ttctttacgt
    12721 tgtatagtgc taaaactcgg acgatgagat gatgctaggt cagaattctt gttcgaaaag
    12781 gtttctgacc ttttctttat atttttgtga ctgacttatt tgatgagaca aactcgctcc
    12841 tcacctgaca agtggattgc tgtgtgagga ggagatgcgg tgaaaaaaga tcgattggtg
    12901 gattttgggt ccttatttga ctttgtccat gatcggtctt ttagaagtat acagtgccag
    12961 ttcttatcgt ttattgcaag ccgatgaaaa tacaaaaagt ctattattgc gtcaactaat
    13021 tttcatattt ttgagttgga gcgtgatctt cttagctcgt tcagtcaaac tacactattt
    13081 acttcaccct aaaatagcag gatacggttt agccttatcg attttctttt tagtattagt
    13141 aagaataggg atattcggtg tcactgtcaa cggcgcacaa cgttggatct ctctgtttgg
    13201 cattcaattc cagccttctg aactggcaaa tctttttttg attttttatt taagctggtt
    13261 ttttcgtgac ggaaatagta gcccaaaaga tctaaaaaaa ccattcctga ttacagtagg
    13321 tataactttt ctgattttat ttcagccaaa gattgctgga gcattgatga tcctttcgat
    13381 tgcgtgggtc atattttggg cagcggcggt tccatttaaa aaagggatct atctaatcgt
    13441 tactttttct gcattgctga ttggagcagc aggcggggta ttatatttag gaaataaagg
    13501 ttggcttcca cagatgttta atcatgctta tgaaaggatc gcgactttaa gagattcttt
    13561 tatcgatagt catggtgcgg gctatcaaat gacccattcg ttttatgcac tatataatgg
    13621 tggcatattt gggcggggat taggaaatag tatcacaaaa aaagggtatc ttccagagac
    13681 agaaacagat tttatttttt cgattattac agaagagctt gggctaatcg gtgcattgtg
    13741 tgtgcttttc ttattgttct cattatgcat gagaatcttt tgtttaagca gtcgctgtaa
    13801 aaatcagcaa gctggattgt ttcttttagg attcggaact ttgctattcg ttcagacgat
    13861 aatgaacgtg ggaagtatcg cggggctgat gccaatgact ggtgtgccat tgccatttgt
    13921 tagctacgga ggaacaagtt atctgatcct ttctttagga atcggaatca cgttgaatat
    13981 ttcttcaaag atacaggccg aagagttggc gctctatcga cctgaaaaac aataaagaga
    14041 aactgagaaa ctctcagttt gaattatcat aaagtacaat cttttatttt tcaagcgaga
    14101 ggttctttta gaaagaggaa ttcgcaatga aagtatctca aaaaatcata ttatctattt
    14161 tgttactgct tacattagta gttggctatt tttcgattga gtttatccat ggattttcgt
    14221 ctgcaaaaca gagttcaact gtgaaaaagg tagagccgaa aagtatccct accacactaa
    14281 atgtggcttt gattggttca gatgctcgtt cgaaagacga aaacggtcgc tcggattcac
    14341 ttctggttgc acaatacaac cagaaaacac aacaagcaaa actgatctct atcatgagag
    14401 attcatatgt cgatattcca ggttacggaa tgaataagat aaatgcagct tactcttatg
    14461 ggggagttga tttattaaat caaacattaa aggaaaattt caaatttgaa gctccctatt
    14521 atgcaagtat cacatttcaa gattttatcg attgcgttaa tgaactgttt cctggtggag
    14581 tcaagatcga tgcagaaaaa tctctagatt tagatggcgt atatataaac aaaggagagc
    14641 aagtgatgga tggtaatact ttactccagt atgctcgatt tcgggaagac aaagaaggag
    14701 acttcgggag gatcagaagg cagcagcaag tgatcaaagc tatctctcag cagctgaaag
    14761 acgtaacttc aatattcaag ctacccaaag cggttgggaa attacttggt agtatccaaa
    14821 cgaaccttcc agaaagtgtg cttctggatt gtggtatgga tttcctcaaa gacatatgtt
    14881 agaataaaca ggtataaata gtgaaataaa ggaatgacaa gcaagagaag gaggaaaaaa
    14941 tgaaaagaag tgacaagcac ggcaaaaatc gaacaggcgc ttatattgcc ggcgcagtga
    15001 tcttaatagc agctgcgggt ggcggttatt tttactacca gcactatcaa gaaacccaag
    15061 cagtagaagc tggagaaaag acagttgagc aatttgtcca agctttgaac aagggagatt
    15121 ataacaaagc tgcagaaatg acatcaaaaa aggcggcaaa taaaagtgca ttatctgaaa
    15181 aagagatctt agataaatac cagaatatat acggtgctgc tgatgtcaaa ggacttcaga
    15241 tatcgaatct aaaagtagat aaaaaagacg attctactta cagtttttca tataaagcaa
    15301 agatgaatac ctcattaggt gaattgaaag atctttctta taaaggaaca ttagacagaa
    15361 atgatggtca aaccacgatc aactggcaac ccaacttggt ttttccagaa atggaaggaa
    15421 atgacaaagt aagcctgacc acacaagaag cagcaagagg gaatattata gatcgaaatg
    15481 gtgaaccatt agcaacaaca ggcaaactaa aacaattagg agtcgttcca agtaaacttg
    15541 gagatggagg cgaaaaaaca gccaatatca aagcgattgc ttcctccttc gacttaacag
    15601 aagatgctat caatcaggcg atttcacaaa gctgggtaca acccgattac tttgtcccat
    15661 tgaaaatcat tgatggagca acgccagaac ttccagctgg agctaccatt caagaagtag
    15721 acggcagata ttatcctttg ggtgaagcag ctgctcagct gattggttac gtgggagata
    15781 tcacagcaga agatattgat aagaatccag aattaagcag taatggaaaa atcggtcgct
    15841 caggtttgga gatggctttt gataaggatc ttcgtgggac aacaggtggg aaattaagca
    15901 ttacagatgc agacggtgta gagaaaaaag ttctgatcga acatgaagtt caaaatggca
    15961 aagatatcaa attgacaatc gatgcgaagg cacaaaaaac agcttttgac agtctaggag
    16021 gaaaagctgg atcaactgtt gcgacaacgc caaaaaccgg tgatcttctt gcgcttgcta
    16081 gctctccaag ctatgatcca aacaaaatga caaacgggat ctcacaagaa gattacaaag
    16141 cttatgaaga aaatcctgaa catccattca tcagccgatt tgcgacaggt tatgctcctg
    16201 gatctacgtt taaaatgatt acagcagcaa tcggtctcga caacggcact atcgatccaa
    16261 atgaagtgtt gacgatcaac gggcttaaat ggcaaaaaga cagttcttgg ggatcttatc
    16321 aagcaacgcg tgtcagtagt gatgtatcac aagtagactt aaaaactgct ttgatctatt
    16381 ccgataatat atatgcggca caagaaacgt tgaaaatggg tgagaaaaaa tttcgtacag
    16441 gcttagataa attcattttt ggtgaagacc ttgatttgcc aattagtatg aatccagcac
    16501 aaatttctaa tgaagatagc tttaactcag atatcttgct agctgatact ggatatggac
    16561 agggcgaact tctaattact cctatccagc aagcagcaat gtattctgtt tttaccaaca
    16621 atggcacact tgtctatcct aaattgattg cagataaaga gacaaaagat aagaagaatg
    16681 tcatcagcga aacagcagta caaacgatcg tgccagatct gagagaagtt gtgcaagatg
    16741 taaatggtac agcacattct ctttctgctt tagggattcc gttggcagcg aaaactggta
    16801 cagcggaaat caaagaaaaa caggatgtaa aggggcaaga gaacagtttc ttgtttgctt
    16861 tcaatcttga taaccaaggt tatatgatgg tcagcatgtt ggaaaataaa gaagatgatg
    16921 attcagcaac caaacgagca tcggaactat tgcaatacct caaccaaaat tatcaataaa
    16981 aaattgcttt ttaagtacag taaaaggact gtggcgttat cgtcacagtc cttttttcaa
    17041 ataaacggtt ttcacgatgt ctctttcaca acttcttttt gatttactca cacttcataa
    17101 actttttttg taaaatattt gagaacaagt ggagaaataa gtgtacttaa taaaatcacg
    17161 ataactaaag gtgaatagta ctgtggttcg atcaattgac tttgctgtcc aatttgtaaa
    17221 atgatcaaag ccatttctcc acgcgaaatc ataccagcac cgaccatcaa tgaactatgt
    17281 tgagaaaaac cggcaatctg agcaccaaaa taacctccaa gcaatttggt caaaatcgct
    17341 acaagcgtca atatagaaat aaagagtagt tgttcactaa agtcagaaaa atcaacttct
    17401 aatcctacac tgacaaaaaa tactggtata aatactgcat atcctaaagc ctctacattg
    17461 tggtatactt cttctttaac ctttgtctga ctgacagcta tcccagcaaa aaaagcacca
    17521 atgacagagc taagcccaac aagatccgct acgtaagaca ttcctaaaca aataactagc
    17581 gatacgatga taatagaaga atttgcgtac actttttctg caaatgaaag taggaaaggc
    17641 gcaatccatt tgactaataa gaaaataaaa acaaaataaa ttagctgttc aatcaaaaca
    17701 agcggtaaag aagcattgct agaagcttcc cccgttagaa aagataggct gaaacttaga
    17761 actaaaacga ctaaaatgtc atctaccaca gaagcaccca aaattgttga tccttctttt
    17821 gtattcacta cgtttagttc ttttaatact tctactgaaa tactgacaga agttgccgca
    17881 agaataatcc caaagaaaat cgcttcattt cccgaaactt ggaatgcttt gcctgtcaac
    17941 cagccaaaaa caaccgggaa gatgatccca agcaaagcaa cgaacatacc aggtcggaaa
    18001 tattttttca aaagagataa atcactttcc agaccagcta aaaacattaa taagatcaca
    18061 ccaatctccg aaaagtcatg gactagaata tcaggtttga cccaattcaa tccagcttgg
    18121 cctagcaaaa caccaacaag taattgtcca atcactgctg ggatacctaa tctccgcgag
    18181 atatgtgagc caatcgttgt tgaaatcaat atcaggcata aaataccaat aaattccatt
    18241 ctcatcattc ctttgtataa aatatagccc gaacttagca taacatacat tattgaaaaa
    18301 ataggaaaat atactcacaa aaaaaccaac aatccaaaag atttttttgg attgttggtt
    18361 tttatacggt ctattttaat aactaaaaat gattaacgta tagtcatttt tcttattctg
    18421 atgaaattga taatctcctg gatgcccatg aaaatcaaaa taaagccgaa tatttggaat
    18481 agaagcatca aacttttgaa tggattgaac aataagacga atccgccaat caacaacaga
    18541 atactactat aaacactcca acttttagct tttgtattca tgctgaagaa caactggaac
    18601 aagctgttga tgataacagc taatccaaga agaatcggca ggatagaaac gattgcaggt
    18661 gcaaaagcat agacgatcag agctaagatc aggaaaaaaa gaccactgaa cagaccgata
    18721 ctccaagaac ctgttttcct tttaaaacgg taagcctcaa aaagattcaa caacccaagc
    18781 aaaacaagat aggcggtgat cagataaccg ataaaatgaa aaacagcaga agggtttagc
    18841 acaatcgcaa tcccagaaat aatataaaca gcagctctta ataaggcgta tttttgaata
    18901 ttttgtttaa tcgtctcaaa catataagaa aacctccttt gacttatttt aacgtgaact
    18961 agtcatttta ccaaattaaa tgttacaaaa tgagcgaagg agaagaatat ctctggaaaa
    19021 atcaacttct gaggatcttt tccatttttt tgccttttgc tagctcatcg atcattttat
    19081 ctaaataacg aatttctttc atcaagttat cctcgatgtc ttctactcgt acaccacaaa
    19141 caacacctgt aatcaaggaa cgtgaaggat tggggacagg ggcttccgca aaaaattgtt
    19201 cgaaagtcac ttgtttttgt agttgatatt ctagttctgt ttgggaataa cccgtcagcc
    19261 aacaaatgat ttcatctact tcctctttta tacgatcttt tctctccgct tttgctacat
    19321 acataggata aacgcttgca aaacttgtcg taaaaatacg attttgcgcc ataatcagtc
    19381 acctttcttt ttttcaaatt atttactact tatagtatat aagaaaaaga gaagaaaaag
    19441 cgattattca aaaaaacaaa aaggattgtg acaaaattct tgtcaccatc cttttttccg
    19501 ctctctgact ttttacaatc ttcatagctc aagtgcgcat cagataatcg aatgctccta
    19561 aagctgcagt agctcctgaa cccattgaaa caatgatttg tttgtatgga ctatctgtac
    19621 aatcaccagc tgcgaaaacg ccggggatat tcgttgcgcc atttttctcg gtcactattt
    19681 catttcttgg attcatttcg atcgttccat ctagccaatc cgtattaggg agtaagccga
    19741 ctagaatgaa tactcctgaa acagctagtg tatgcatctc catcgtacgg cgatcgatat
    19801 aggagagaga ttctacctga ttcgtaccgc tgatttcttt cgtcgctgca tgtttgataa
    19861 cagtgacatt cgcaagagag actagtttat cctgtaagat ttgatctgct tttaattctg
    19921 gtaagaattc tagaacatat acatgttttg ctaacccagc caaatcgatt gctgcttcaa
    19981 caccagaatt accaccaccg ataacagcaa tctcttttcc tttaaataaa gggccgtcac
    20041 agtgtgggca ataagcaatt cctttatttt taaactcttt ttcgcctggt acattgatag
    20101 aacgccagtg cgcacctgtc gcgatgatgg cggtttttgc tttaagtgat ataccgttag
    20161 ctaagccaat attaataaaa tgttgctctt tatttaagga aacggcctgt tgattcatca
    20221 taatgtcgat agggtaacta cgcacatgac tttctacttg ctgcatcaat tttggacctt
    20281 ctgtgtaaga ggtaccaatc atattttcaa tccctagtgt ttcaacgact tgtccgccga
    20341 aagaatcagc gacaagtcct gtacggattc ctttacgtgc tgcatagata gctgcactac
    20401 tagctgctgg tccaccgcca atcactaata catcataagg ttctttttca aacaactctt
    20461 cttcaatcaa agggccagtg attttttcaa caatttgttc gatcgtcatg cgtccgctac
    20521 taaattcttc tccatttaaa aacacagttg gcactgccat gattttttta tcatcgattt
    20581 ctttttggaa catcccacct tcaatcatcg tatgagtaat atttggattt aatacagcta
    20641 atatattcaa tgcctgtaca acgtcggggc aattatgaca agtcaagcta acataactgt
    20701 caaaatgcaa tgtttgtgtt atttttttga ttttaagact caaagattca ctgattttcg
    20761 gtgcccgtcc actaatttgt aacatagcca gtaaaaaaga ggaaaattca tgaccgaggg
    20821 ggataccagc aaaaacgatt ccactttctt tgccaactgg attgatagaa aaactaggtg
    20881 tgcgcatcga atctcttttt tcccaagaga ttttaggaga catttctgct atttccttcg
    20941 taaattcagc tagtttttga ctattttcac tattatccaa actaacagaa aaaacaatag
    21001 gagattcaat cagtgctaaa tattgttcga gattttgttt agtttctttg tctaacatat
    21061 ccaccaccta aatctttcca acaagatcaa aacttggttt caatgtttcg ccactttctt
    21121 tccattttgc cggacaaact tcacctgggt gggtacgtac atattgagca gcgcgaatct
    21181 tatcaatcag tgcactagcg tcacgaccga ttccatctgc attgatttcc atagcttgaa
    21241 cgattccgtc tggatcaata ataaatgttc ctcgctgagc taatccttgc tcttcatcca
    21301 aaacatcaaa aatacgagaa atctgatgag acggatcacc gatcatcacg tattcgattt
    21361 taccgatagc atctgatgta tcatgccatg ctttgtgagt aaagtgggta tcggtagagc
    21421 aagaaaagac ttcaacacct aaagatttca atgtactgta ttgttcttgt aagtcttcta
    21481 attcagtagg acaaacaaaa gtaaaatcag ctggatagaa gcaaagaata ctccagttcc
    21541 ccataagatc tttttcactg acttctaaaa attctccttg atgataagca tgtgcattaa
    21601 attcttcgat ttttttccca atcaatgaca tagtaaaacc tccaataata attatttatt
    21661 tataattatt gtaatttagt tggagttgca aagtcaatgg atattttgtt atttaataaa
    21721 gattactcaa aaacgaaggt taattataaa aatagaagag gtcgcaagaa aatgctactg
    21781 ctggaaatac tcgggataac gttgagtaac agtttcctgt tctttcagca tcatgtgtaa
    21841 gtgcaatccc gtttgaaatc gagcttcttc tggtttttta tcaactaaag ccttgagtaa
    21901 atcataatgt tgttccatga tcgtttccca tggaagcccc tgaatggtca ctcgtaacca
    21961 gcggaaacgt tctaaatggg tattatggtc atctaaccag ttccaaactt cttttcttcc
    22021 tgcaatttcg aaacaagctt catgaaataa attatctgca cggaaaaaag cgcgttcgtc
    22081 atttattttg actgcttttt cttgttcttc taaaatcgtt tctagcacta ttcgtgtttg
    22141 tgtgtccaat ttggaacaac attcaatcat gatttgctct tccaactgtt ctcggacaaa
    22201 gcgagcattt tctgcagaaa caagattaat tttggaaaca tacgtaccac tttgaggtat
    22261 cgtatacacg agttcttgct gacgtagttg aatcaatgct tcgcgaatgg gagtccggcc
    22321 gatagcaaga tgttcctcca gtgccttttc agatattttc cttccaggtt ctaagtcagc
    22381 atagataatt tgtttacgaa tcgcttgata agcttgtgat tgcaagctgc ttttcatctt
    22441 tattcctcca aaatcatttc tatgaacgta gtataacaga agaggtctga aaaacaatct
    22501 ggtatgtcac attaactagt aaatttgtat aaatagtaaa attttggttg acatagcgat
    22561 ttttattgca taaaattcac ttgaatacaa aaactaatat gttagtttag gaggggaaaa
    22621 atgattgatt taaacaactg gaggaacttt atccctgaat tatctcaaaa agaagtagaa
    22681 atacctcatt ttgaccgtgc atcattaaga gaaaatggac aaaaaaatcc tgtctggatc
    22741 catttcggtg gcggaaatct ttatcgagga tttcatgcag aaatcgctca acgtttagcg
    22801 gatctaggcg aattgtccac tggtgtcatt gtttgtgaaa cattcgatga agaaataatt
    22861 gaaagggctt accaagattt tgatgatttg attcttgaag tggtcatgcc aagcagtgga
    22921 gagttcgtca aacgtatatt agctgcgaca gctgacagtc tgtattgtca tccagattgt
    22981 tctgaacact ttgataagat gattcgacac tttgaaaatc ctagtttaca attagttact
    23041 tttgctatta ctgaaaaagg ttatcgattg aaagatacaa ataataaata tataggaccg
    23101 attgcagtcg atataaaaaa tggtccaaag aagccatcac atacaatgag tattcttgct
    23161 gtgctacttt ataaacgttt tttatctgga gcttggccga ttgcacttgt ctctacagat
    23221 aatttttcac aaaacggtag tcaattacgt acttctttgc taacgatcgc aaaagaatgg
    23281 caaaaggaag gttatgtaca aaatacattt atccaatact tatcgaatga agaaattgtt
    23341 gcgtttccat ggacgatgat tgatcgtatc accccgaatc cttccttgtc agttagcaaa
    23401 atgttggcaa aagagggctt ttcaaatatg tcgattttcc aaaccgcaaa aggaaataat
    23461 attgctccat ttgtcaatac agaggacatc cattatttaa tcatggaaga tcattttccc
    23521 aatggacgtc cgaaattcga aaaagctggg gtattcttga ctgatcgtgc aacggtagat
    23581 aaagcagata cgatgaaggt gacgacttgc cttaatcctt tgcatacgtc tttagctatt
    23641 atgggttgtt tactggggta taggagcata gctgaggaga tgaaagatcc agatttatcg
    23701 ctgctagttt ctgaaatcgg gtacaatgaa ggcctcccag ttgtggagtc tccaggtatc
    23761 attgatccaa agatctttct ggaagaagta attactgtgc gcttttctaa tccaaatatt
    23821 cctaatatgc ctcagcgaat cgccacggat acctctcaaa aggtggcgat tcgttatggt
    23881 gagacgatta aaaaatatat ggaaatagaa gttaaagacc cgcagacact tgtattcata
    23941 ccattgactc tcgctgcttg gcttcgatac cttattggca ttgatgatca aggaaaacca
    24001 ttcatcccga gtccagaccc actattgaca gaccttcagc ataagctttc cgatattcaa
    24061 ctgggtacgc aagatacatc acatattcat gagaaaatgg ctcctatttt gtcaaataaa
    24121 gaaatttttg gtgtagatct ttaccaagca gagcttggca gaaaaataga agaaatgttc
    24181 atcaagatgg tagcaggacc aaatgccgta cgaaaaacat tacaggatta cttacaagaa
    24241 catggaggaa atttatcatg gaaatgactt ttagatggta cggatcacag gaagaaaaga
    24301 ttaccttaga tgaaatcaga cagattcctg cggtcaaagg gattgtaggc acattgatgg
    24361 atataccagt aggagaggta tggcctaggg agaggatcaa agaactaaaa gaagaaattg
    24421 agtctcgtgg gttaacacta aaagtaattg aaagcgtcaa tgtgcatgat gccattaaaa
    24481 ttggattacc ttcccgtgag cagtatatcg aaaactataa gcagacaatc cgaaacttgg
    24541 cagaatatgg aatagaagtc atctgctata attttatgcc tgtatttgac tgggtgaaat
    24601 ctgaccttga ctaccgctta gaagacggct catccacgtt agcttttata tctgatgata
    24661 ttccagatga tccaagagaa atcgtcgcgc gcatcgaaca ttccagcaat gaatttggat
    24721 tgccgggatg ggaacctgag cgcttagctc ctatcaaatc cttgcttgaa gcctatgcat
    24781 cagtagacga agaaaaactt cgagaaaatt ttgcctattt tctccaatca atcatcccaa
    24841 catgtgaaga ggtaggggtc aaaatggctg tacatccaga tgatccgcca tactcgattt
    24901 ttggattagc tcgaatcgta aagaatcgag aagatttaga ttggttgtgt cgcatagtag
    24961 acagccctta taacggaatc actctttgta cagggagtat tgcagaagat ccagaaaatg
    25021 atgtatatgg gattttagca gaattttgcc aaagagatcg aatcccattt gcacatgttc
    25081 gaaatattaa atttttagct aatcgagatt tctatgaggc accacataaa tccgaatacg
    25141 gttcattaga catgtaccgc atactaaaaa caatgtatga caacggattt aacggataca
    25201 tccgtcctga tcatggaaga atgatctgga atgagacagg aagaccagga tacggcttat
    25261 atgatcgagc attaggtgct gtttatctaa atggattatg ggaagcaatc gaaaaaggaa
    25321 acaaaagtaa agataattag tgaaaaaaac tgtaatttta ttatgtaaat aagaaggatg
    25381 ttcaaattct tgatacataa atccggaggt tgttaataag cattcggatt tatgtatctt
    25441 atttggtcta ggaagtaact tgattttctt ttttcgattg atccatgagt gcttcttctt
    25501 ttaattcaaa gatatattct gagatcctca taatcacttt tttccagtct tctggtgtga
    25561 tgattccttt taaaatatag atttttttga tatgagaagg caattgtttc aaagggcagt
    25621 cgctgataac gatgttgcag ttctccataa aatcattttg tattttgatc gattcatttg
    25681 aaaaaatttg ttctatctta acgatcagat ctttactgat ataaaaatca cgcgtatagt
    25741 tcacaccaat cacaattcgc ggctgttttt tgtagcgatc atacaaatgt actaggtctt
    25801 caaccatcca ttgcacattt tccttttcta aaataactgg taacaagtgt tgattttgtt
    25861 ctctgaaatc catataaaat tgttcaatgg cggtttggac ttctttcaat acgctatgtt
    25921 cttgataaag catgaaagta ggaaggggcg tatttggttg attgaagtta agaaatccat
    25981 tataaagttt atttcgtgcg aatgccaatg agtaaagaat cttttctttt tcaggtataa
    26041 acaaatcaaa tacagtacaa agggactcca ccatttctct tgaataaaga acatgaggaa
    26101 cgtttgcttt ttttaaaagt ttgtattgct ccaagcttgt ttcttccgat tcgattttcg
    26161 tgatagaaag tcgagctaaa atattcacta agatgcgttg ttcgcgacaa attggcagat
    26221 taggtcgcaa aatatctaca gagagatcaa taaaaaagtc aatatattca tactctgtga
    26281 attctttttg tacgtctttc acagaagttg caggaaaatt ctctttacat atttttaata
    26341 gaaaatataa tttatctatc ataccgctat ttagtcggtc aagtacttct tttttaaaaa
    26401 aagtattcat cataaaagcc actacttgcg gatcttcatc ataaaattct gtagcagtac
    26461 ttgaaaagat gttccaataa atatctaatg ctgcatattg aatttgtacc tcttttccag
    26521 aaaaagactt tctgccatta ttagaaaagg agatagacaa accgtactat gctaaaaatg
    26581 cattaatctt tttcattttc gtatacaaat aagaggcact atagtttgtt tgctgactga
    26641 aactgataat cgaatttgtc tgacagctga acaaagaagt caagatctga taactgactg
    26701 attcgttgca gtatcttcca aatagacgat aatagcagta agccggatca attccttcaa
    26761 aattcaactt taaaaaaggt ttttgcttat caatatgcag ttctgttaga ttgagacgct
    26821 caaagtcttg ttctaattct ttgatgtata gtaaaaaatt tgattcagac atatggaaat
    26881 gatctgctaa atattgtgta gtaataaaga aacgatgttc agataggaaa agaaaaatat
    26941 ctaattttgt attgcttttt ttcttcaaga taaacgtttt aatcgcttct tgagtttgca
    27001 tgaaagaggc tcctttttac ttaaattgtt aaaacttgaa aatattactg tttttttcat
    27061 aaatttagac aaaaaaacag taaacatata ggtagaatct ctactatctt atttcttgca
    27121 tacaaaaaaa tcaaggagtg agagagttat gagatattca accatcttaa tatcaacatt
    27181 tgcagcctta agcttatcgc ttactataaa tcagacagtc cacacagagg agacagaaac
    27241 agccaaatgg acagaaacca agtgggccaa cgtcaatcga attacctttc aggatgaatt
    27301 ttcatccttc aattcaggag tcgcaaaatt aagaaaagga caaacagcga ccatcaaagc
    27361 aaaaattgac tattcgggag acttagcacc tatactacaa tcagctgtta catttgagaa
    27421 tgttgatccc actttgtcga ttggctatga cgagaacact ttgactatcc aacacaagca
    27481 agcgatattt accttcaccg ttacattaaa tcaggacttg ttaaaaccag cgctttttac
    27541 tgtcaaggta tcagatggac taccgaacga tcatcattta acgccgtata gtcagcggca
    27601 gacaattgag caagattcag ccatcgactc aggaggagat cttgttgaag aaccaacaga
    27661 taaaccagaa aatgaaaata agccagaagt accgcctaca gaaaatccag acggagaaca
    27721 gaaaccggaa atagaacctg gagaagaacc cgatacagaa actcaaccag agccagacaa
    27781 taaatcaaaa cctgaaataa cccctggaga aaaaccagat gtaactccag aaccagatac
    27841 ggattctgga aaccaaacag taccggaaac aaatccagac acagataatg aaactgaaaa
    27901 tccagaaaaa ccagaggtag atccagaaga aaaaccagat gtgactccag aaccagatac
    27961 ggacgcaaga gaccaaggaa taccagaaaa aataaacaaa aaaacaattc aagaggatgg
    28021 aaaaaaagag agtaaaaaat caaatctagc aattctaaaa ataaatgaag agcagctgaa
    28081 taaaaaatca cgaatatttg atagcgccca atcagctgaa accttaaaaa gtagcaagga
    28141 tacaaccttt gctgtcctga aacgaaaaat aaacagctac caaaatccgg agagtcacaa
    28201 aacaaagtta tactgtggag cggaatcatt ctattaagta tagcaacaat gctttctgca
    28261 aaaagattta aacaaaacag atctctctaa catttgaata atgtctgtga acgcattttt
    28321 caaataaaaa gaaatatcca gaaaatgacc ccaaaatacc agattgaaat ctgatatttg
    28381 gggtcgttca tactttatct ttcttcatat ttattttaag ggatatctgt ttcttttgga
    28441 agatggttcg ggatcttttt cactttcaat acatctaaaa agaaggtaaa gatcggaatg
    28501 ccgacaatca agccccaaac gccaaagaac cgttcgctaa caagtaagac cacaaatgta
    28561 taaaaaatcg gcagttctgt tttgctggac atcaacttcg gattcagcac ataagactca
    28621 attagatgga caatcaaaat cgtaatcagg atataaacaa catctcggat gcctccttgg
    28681 gaataagcta tgaaagatag aggaatacaa gaaatgataa cacctgctac tgggatcaga
    28741 cttaaaatga aaatcatgat cgctaaactc agcagctgat gaaagccaaa tacagccaag
    28801 gcgattgtag tgagcaacgt atttaaaaga gcgacaacaa attgagcttc taataccaaa
    28861 ccaaatgtat tgacaaactt atcggcaaaa tagtaaatat cttgaaagaa ccaagaaaat
    28921 tcacttttta aaaacaatct tgagaataca attgtttttt tcttttcaat cataaaaaag
    28981 aaacttaaga tgaatgacat agcaaacgcc actgccattt ttcctatatc ctgtaaatag
    29041 ccaagcgcaa tcgaagcgcc attttgaagc tgttcaaaaa aatcgtttcg ctcaatataa
    29101 gtatggatat attgtaaaac aggatctgca tcttttggct gatgctcgta aaaattaata
    29161 acggactgga tcatgtcaaa tgtttgatga ataagcatcg ggatatactt tgtgaatgcc
    29221 agatagataa ggtaaacgat cagtgtataa atcatgatag ctaaaacacc tgtagggatt
    29281 cggacaaatc gctgaatgat ttgaacaaga tgaatcatca aataagtaaa gataaatgtg
    29341 agcaaaatgg ttgtaatcat acttctaact aagaaaagaa caaaaacgac aaataataaa
    29401 acagtgagac ggcgtatttt ttcattcgcc aaaaaacgct gataaacggt caaatcgaca
    29461 acctccaaat aacaaaaaat aaacacgtta ttcattatat gatggaatat gaaaataaga
    29521 aagagcaaaa agaaaaaccg ctaaatgacg ttatcttatg agttggtttt cctcataaaa
    29581 tcgttcatct agcagtttca gccctttttc tggtcttgat gaaaattatc tagcattttt
    29641 cgggtcagat gttgcagtgt atccaattct tcctgttcaa gtggaagata agacttgatc
    29701 ttttgtggaa taccttctgc ttgtttttgc aacgcagcac cttgagcagt cagcgtaaca
    29761 tttacgattc tttcatcacc cgtttcacgc tgacgattaa tataattttt ttctgctaac
    29821 ttttttagta agggagtcag cgtaccagaa tccagacaca aatcatttcc tagctctttc
    29881 actgaagtct ttttcttttc ccataaaact aacatcacaa gatactgggt ataggtaaga
    29941 ttcagcggct tgaggagggg agtgtagtgt ctaatgattt ctttagaggc ggcatataaa
    30001 ggaaaacata cttgattaac taaatataga tcttccattt ttccacctcc gctgttttac
    30061 tatagtgtat catagcggat ttacacaaaa aagtctattc actaactgct ttcagagtaa
    30121 cttctatatt tccagctgta gcttttgaat acggacacac tttatgcgct gcctctaata
    30181 attcttgcgt ttcttccaga gaaagacctt cgatagatcc ttcaatctct acacctaaga
    30241 caaagctcgt ttcactttct gaataaaggg aaacttgtgc actgacagta gaagccccag
    30301 agatattttt ttgctgtttg atcaattcta atgcactgtt aaaacatgca ctgaaaccag
    30361 ctgcaaacaa ttgttcaggg ttggttgcat tttcgacgcg tttaccagga gctgcaatat
    30421 tcaattcgaa tgtacgatcg ggattatgca cttctccaga gcgtccgcct gtgttgatca
    30481 ttttcgtaga atacattttt ttcataaaat taccaccttt aattatattt tatacaattg
    30541 aattttacac aatataattg tggaaagcaa agaaatggat tggcataaga taaataagag
    30601 aaagcacatg aaacagacgc gctttctcga atagcaaaca tatacaaaaa atatcagaga
    30661 caaaaaataa tctcatcata aacattacat atatttctca aaaagttgtt ttctttcttc
    30721 aaaaattact tcacgatgga cctgatgaag catccataaa taaccaaaag gatctctgaa
    30781 cattgagtta gtcacaccaa agtccatcat ttctgtcatt tcttgaatct ctacacagcc
    30841 atttcgtaat gctttattat gtgtgtcctc gatatcagag acgatgatat tgaaccagat
    30901 cgtatttgga tgttcagcag taggagcagt caaaccaaga gacgtgtttt catccagcat
    30961 atggaatctc gtaccataca aggtaaaaat gacttcatta ttgcctctag ggtaatctgt
    31021 tgcctcaatc agttgtattt catcaaagat tgtttgatac agttctagtg ctttcaaact
    31081 atcatttaat acaaaatcga tttccgttcc tttatgcata aagatctcct ctccttttca
    31141 gtcgaacagt tactagattc ttttattata ccagctaaag aaaacgcatt caaaaaaata
    31201 gcggatttac ttaccaactc attcgattgg atgataagta aatccgcgtc ccattacttc
    31261 tgctacgctg ctgacaatga caaaggcact tggatcgatt tcttctatca atggtttcat
    31321 tcgggaaaat tcatgatcag tcacaacgat caataaaacc tcttttgact ttcgattata
    31381 cccgcctttt gcctctagca gggtaaggcc acgtccaatc tcagcactta agcgaaggcg
    31441 gatgtcttcc aaataatgtt cactcatgac cataacagat ttctttcgat tgatacctgt
    31501 ttcgatataa gtcatgacaa ttgaagtgat tgcaatagat aaaatagcat aaagaaactc
    31561 ttctattcca aaaataaaca gattaagaga aacaacgaat gcgtctgtaa aaaataagcc
    31621 aatcgaagta ttcaagtgga aatatttttt aaaaatgagg ggagggatcg tcgtaccgcc
    31681 gctagaagag ttgtttttat ataaaatcgc aactcctaaa gcaaaaatcg cacttccaaa
    31741 aatgacagac aacaaccgat ctgaagtaat cattgtttct ggaactattg caatgattaa
    31801 aggaaaaacc aagcttccga aaagtacttt aaaaaagggt tttttaccta aaaatagaag
    31861 agctagtacc aacataacga tattcaacac taaaataact atagctcggt caaagcctaa
    31921 agcagcttcc agtaatattc ctagaccgct gacacctcca gcagcaatat gatgaggccc
    31981 taaaaacata ttgatagcga ctcctaatat gaagagggca aaaataatct taatgtatgt
    32041 ccatataaat tgtctcgttt ctttcattta ttcacttcct taattcatta ataatactct
    32101 tatcttatta taacttactt ttcaccttaa gttaacttta attaataaaa aaattaggct
    32161 agtctaaatt attatgtttc attcttctag aatatacttc tacgccaaaa actgttaaaa
    32221 aacttttact ttagaaaatt ggagaaattg atcgcttaag ataaattcag gacactaaat
    32281 agtacaggaa gtagactaaa acttttaaaa gttttaagtc tatctatttg gcaagtttaa
    32341 aaaataaaaa ttcggtatca aaaaactgtt accgataaca tttttgaaag cgttgtcttt
    32401 tgcttctcta tcacttatag tgatgacgtc atattaaaac acaaggagga aatcaatgga
    32461 tacagctgca atttatcatc gaccggaaag tgaatatgca tttctatata cgaatacaag
    32521 atttcgtatt cggcttcgta ctaggaaaaa tgatatcaaa aaagtatatg tgctatgtgg
    32581 agatccatat accataacaa ctgaaaaatg gtatcaaaaa caacgaccaa tgaaaaaatg
    32641 tctaagtacc aatgttcatg attattggga aattgaagtg acgagcgaaa caagtcgctt
    32701 acagtatgcg ttccatgttg ttggggaaga taacacggac tgtttttacg gtgatcaagg
    32761 aatttttcct tatcgagaaa atgtgattac tgaaccaaat ttttattttc gaatacctta
    32821 ttttcatcaa atcgatcgat tcactattcc agaatgggta aaagaaacag tatggtatca
    32881 aatttttccc gaaagatttg caaacgggga caaagcaaat gaccccgaac acactttacc
    32941 ttggggaagt aaagatccag accgagaaga ctttttcggg ggagatttgc aaggtgtgat
    33001 ggatcatttg gattacttga cggaccttgg catcaatggc atctattttt gtcccatatt
    33061 taaagccaca tccaatcata aatacgatac gattgattat tatgaggtgg atcccgcttt
    33121 tggcgataaa aaacttttaa aaaacttgat tgatgaagca cataaaagag ggatacgtat
    33181 catgttcgat gctgtattca atcatatggg tgtccattca ccacagtggc aagacgttct
    33241 caaaaacgga gaaaaatcga tctataaaga ctggttccac atccgttttt tcccagtaga
    33301 ttcttatcag atgactgact taccagaaac tgctgaaaat atcccatatg atacttttgc
    33361 ttttactcca ttcatgccga aactaaatac agcaaatcca gaagtgcaaa aatatttgct
    33421 ggatatcgca acttattgga taaaagaatt cgatattgat ggatggcgtc ttgatgttgc
    33481 taatgaagtc gatcatcatt tttggaaaaa attcagagaa gcagttacag aaatcaaacc
    33541 agacatctat atccttggag agatttggca ttcctctcaa gcttggctgc aaggggatga
    33601 attccatgca gtgatgaatt atgcatttac agattcgatt aaagattatt ttgcaaaaaa
    33661 gaaaatcaca gcttcacaaa tggtcagtgg aatgaatcat cagcaaatgc tttacagaga
    33721 tcaggtgaac gaaggaacat tcaacttatt agattctcat gatactgcca ggatcttgac
    33781 cctttgccaa ggtaacaaag aattgatgaa gtctgtgatg gcctttatgt ttcttcaaaa
    33841 agggtctcct tgtatctatt acgggacaga aatagggatg actggtgaag atgacccgga
    33901 ttgccgcaaa tgtatgatat gggagaagga ggaacaagat ctagagctat tcggttttat
    33961 aaaagaactt gtttctctaa ggaaacagtt gtctaagatt atttctgaag gttctactca
    34021 gtggctaata gtaaatgatc gtgaagataa gctctatttc acacggaaac tagagggaca
    34081 gatcatctat gtttacttta atcagtcaaa agaaccttgg gtagtagaac aggaaaatga
    34141 ggtgattctt agtcaaaatt gtcagttgtt agaagacggg aaagcagaga tacgacaata
    34201 cggatttttg atcatgtgtt gaaaactgtt accggtaaca acaagaaagc gtttgcaaat
    34261 gaagatttgt gcccattata ataagggtgt aacagcacgt tgaatacaaa ggaggaaatg
    34321 aagatgaaaa aaagaagtgt gaaacagttg ggtttagggt taatctcttt aggaatctta
    34381 ggcggattag cggcttgtgg caatggtaac tcttcagcag aaggggaagc cgatgaagga
    34441 aaaacgttaa cagtttccgt tgattctgga tataaagatt atataaacga aataaaaaac
    34501 actttcgaaa aagaaaacga tgtgaaaatc aagctagtag aaaaggacat gttcgatcag
    34561 ttggaatcat tagcgttaga tgggcctgct ggaaagggac ctgacgttat gatggcagca
    34621 tatgaccgga tcggtgcgtt aggacagcaa ggacatctgg cggaagtaaa actgggaaat
    34681 gaagcagctt acgatgaaac agacaaagcc caagtgactt atgatggaaa aatctacgga
    34741 gaacctgcag taatcgagac ccttgttttg tactataaca aagatttagt cgatacagca
    34801 ccatcaacgt ttaaagattt ggagaatcta tccaaagaca gtcgttttgc ttttgagtca
    34861 gaagcaggaa aaaatacagg atttttagct aaatggactg acttttatta ttcctatggt
    34921 ttaattgcag gttacggtgg ctatgtattt ggtgatgatg gcacaaatcc ttcagatatc
    34981 ggtttgaata atgcaggtgc tgtagaagga atctcttatg caacagattg gttcaaaaat
    35041 gtttggccaa agggaatgca agacataaaa agtgcaggag acttcgccag ccagcaattc
    35101 atgtccaata aaactgcagc aatcattgac ggtccatggc aagcgcaaac atacaaagaa
    35161 aacaatatca attatggtgt agccaaaatt cctactctaa ataatggaca gccttatcaa
    35221 ccgtttggcg gaggaaaagg atgggtagtc agcaattatg ctaaaaacaa agacctctct
    35281 caaaaatggc ttgattatgt aacgaatcag gaaaatcaag aaaaattctt tgaaatggta
    35341 aatgaaatcc ctgcaaacca acaagctagg gaaacagcaa aggctaaaaa cgatgaatta
    35401 acaacagctg tgattgaaca atatgaaaca gctcaagcga tgccaaacat cccagaaatg
    35461 ggcgaagttt ggacaggtgc agaaaacttg atgttcgatg cagcatcagg ttcaaaaaca
    35521 ccaaaagaat ccgcagatga agcagtaaaa acaatcagtg aagccataga acaaaaatac
    35581 tgattaaaat gataaccgtc catttttata aaaaatggaa caggtgcttc agataaaagt
    35641 ggcaagagcg agatggaaaa ttatcttgct cttgttgcca ttatcttaaa agcagtttgc
    35701 ggtttagcaa gtttagaggg ggcattcaga tgcagcaaga taaaacaagc aatatcaacg
    35761 aaaaaaaaat cacgctttgt tcacttattc cagggatggg gcagtttgta aatggccaag
    35821 cttttaaagg tatcgttttt ttcgccatat tcgtcctatt cattggtcag atgcttctgg
    35881 gcggatttca tgcattggta ggattggtta ctttgggaag tataccaatg gaagatcatt
    35941 cattatttat tttgattgaa ggcactttgc aattcatcat tacaatcatt ttcttgattt
    36001 tttacggttt gaatctttta gacgcttatc gagtagcgaa gatgcgtcaa gagggcaaaa
    36061 aaatcaatcg gacgatgaag gaagtcatac ataatatagg tgatgaaggt tttccttatc
    36121 tattgacact tcccgcttat cttctcatga tttttacgat cattttccca gtcctagtta
    36181 cattgttcac tgcatttact aattatgact tcaatcatat tccaccagcc agtttaattg
    36241 attgggtagg ggctaaaaac ttttttagta tcttcttttt aagctcatat cgtaatactt
    36301 tttttgcagt cttttcgtgg accgtgattt ggactttatg tgcaacaact ttgcaaatca
    36361 cccttggtat tctcacagcg gttgttgctc accagaaatt tattaaattc aaacgtattt
    36421 tcggtatcat tttcttatta ccatgggcgg tcccagcttt tattactatc atgagttttt
    36481 cgaatatatt caacgatagc gtaggtgcca tcaatactca agtgattcct ttcataaacc
    36541 agcttccttt tgtagatatc agtgcaatct cttggaaaac ggacccgaac tggacgaaag
    36601 tagcaattat cttagtgcag ggctggttag gatttcccta tatctatgtg atggttacag
    36661 gaatcttaca atcgatttca gaagacttat atgaagcagc aaaaatcgac ggagcaaatg
    36721 catggcagcg cttttcaaac atcacgcttc ctgctgtctt cctagtggca gcgccaacat
    36781 ttattacaca gtatacgggg aatttcaata atttctccat gatctatctg tttaacaatg
    36841 gcggtcccgg aagcgtaggc ggaggagctg gttctactga tattcttatt tcctggatct
    36901 ataaacttac gactggaact tctccgcagt attctatggc ttcagcaatc acgttgatta
    36961 tctccgtcat cgttatctgt gtgtcattgc tggtatttaa aaagacaaat gcgttcaata
    37021 tgggggatta acaatgaaaa aatttcattc gagtgctcga ttccaaaaac gactttctca
    37081 cttttttact tacttttttc ttttaatact gtcagtcatt atcatctatc cgcttttgat
    37141 cacagcaagt actgccttca agtcaggaaa tattgcagct tttagtttag atctttcaag
    37201 tgactggact ttaggaaact ttactcggct attcagtgaa acactttacg ggacttggta
    37261 taaaaataca ttgatcattt ctgtcctaac aatgatcgta caagtgactg tagtaacttt
    37321 ggcgggctat acttatagtc gctatcgttt tatcggccgc aaatcaagtt tgatgttctt
    37381 tttagtgatt cagatggtac cgactatggc agcattgact gccttttatg tcatggcttt
    37441 cttattagaa gcgctcgata agtactggtt cttgacaatg atctatatag gcggtggaat
    37501 tccaatgaat acttggctga tgaaaggata ctttgacact gtaccaatag atttagatga
    37561 atctgctaaa cttgatgggg cagggcattt caggattttt ttccagattg tattaccgct
    37621 tgtcaaaccg atgctcgccg ttcaagcatt atgggctttt atgggacctt ttggggattt
    37681 catgttagct aagttcctgc ttcgaacacc tgaaaatcag actgttgctg tcggcttaca
    37741 aacatttatt tctgatgtga caaaccaaag agtctctctt tttgctgctg gggcaatttt
    37801 gattgcacta ccgatttgtc tattattctt ctttttacaa aagaactttg ttaccggcct
    37861 gacatctggc ggaacaaaag gataggagca gagtatcatg caaacatttc cgttaaacta
    37921 tttttccgca ttacgtacgc caactcaagt gtttgcggga cgcaaactat tatcatggcc
    37981 taaatttttc ttgatatttg tatttttagt ttccttgatg gtcatgcctg tgactctttt
    38041 ttatgccaat cagatccaag cgattccatt agaacaattt ctatccgttc attcgctgat
    38101 cgatgaacaa ggaacccaaa aattttctga acttgaatta tctgaaactg ggttacaagc
    38161 atcgcaacaa acgatagctg tgacccctga aattttggta ggtgtgtctt tatctgagaa
    38221 acaacaatcc gatcatggaa cttttattga ttttgagaag gaacaatggg tgatccaaca
    38281 aaaggataaa tctggtatac gccgctatac gatgaattat agcccatctt tccaaccaga
    38341 ttctgtacgt acgcccgaag atttccaacg atttttagaa agagaatttt atgccagtaa
    38401 tcgtccaaca atcattttat cttatagttt aagtcttggt cttgtcctat ttgtcatgac
    38461 gagtctcatt ttattcgggg cttccttttt cttgtggatg acaagaaaaa gccaactttc
    38521 atccattcat acattcaaag agagtgcaaa tttgatgctg aatgttatgg gaataggttc
    38581 catggcagca gcagttgtag ggtttatcca ctttgacttt atcttgatgc taagtgtcca
    38641 aacaacgata actgtcttgc tgcttttatg ggtttttgtc aaaacaagat tcaaagataa
    38701 gcgtgtcgaa tgacaagttt aagcgattta tgcgacaatg aatgtgaaaa aaatgaaaaa
    38761 taaagtagtt tttgaaaaat ttctgaaaaa attaaacttc ttagttgatt cttttctttt
    38821 ttagtattaa cgtaataggg tagttttttt aaggaggaaa atcatgttag agaaaacttt
    38881 atacaatcaa ttattttcac gttctttctc gctccctgtc gaagtaaatt attgggacgg
    38941 tacaacgaaa caatacggag atacggacac tcctccccaa atcaaaatca atatccatga
    39001 agaaatccca atgaaagaga ttacaagtaa cgcatctttg gcactaggag aagcctatat
    39061 ggatcatcga atcgagatcg agggaagtat ccaagcattg atcaacgatg cttatcagca
    39121 agcaaacagc tttttacgag gaaaagatta tctaaaatgg ctgccaaaga aagaaaaaca
    39181 tacgaaacaa aaaaacaaag aagatatcca tgcgcattat gacttaggta acgactttta
    39241 taaactttgg ctagatccaa ctttgactta ttcttgtgct tatttcaata cacctgaaga
    39301 tacgttggaa caagcacaaa tcaataaagt ccatcatatc ttagacaaac tctttatcaa
    39361 agaaggcgaa acgttgcttg atatcggttg tggctgggga acattgatgt tcactgctac
    39421 caaagagtat aatgtcaagg caactgggat cacactaagt gaagaacaat acgatttgat
    39481 taccaaaaga ataaaagaag aacatttaga agataaatgc cgtgtattat taatggatta
    39541 ccgcgaacta aaaggcgaaa cgtttgatca tatcacaagt gtaggtatgt tcgaacatgt
    39601 aggttctgaa aatcttgaag gttatttcaa agtggtaaaa gatttattga aaccaaaagg
    39661 aacagctttg atccatggaa tcagccgaca acaaggcggt gcgacaaatg cttggatcaa
    39721 taaatatatt ttccctggcg gttatgtccc tggtgttact gaattagttg ataatattac
    39781 aaaaaacgat ttgcaactga ttgatttgga aagcttacgt cgtgactacc aattgacatt
    39841 agaacattgg acaaagaatt ttcatcaagt ccaagatcaa gtcacgaaag aaatgggtga
    39901 ttccttctat cgaatgtggg atctatattt acaagcttgt gcagcttcat ttgaatcaag
    39961 taatatcgat gtgattcaat atttgcttgt tcaacctgga aacaatgaca tcccaatgca
    40021 tcgtgcataa ttgaggcaaa agtactgttt aaagactatt taaaaagaag ctaagtttta
    40081 tgatgctcca gaagtgaact tttttgtcta acttctagga ttgtctctaa agcttagttt
    40141 c
//
LOCUS       DAFEOM010000019        39008 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.019, whole genome shotgun sequence.
ACCESSION   DAFEOM010000019
VERSION     DAFEOM010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.019"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            6..305
                     /locus_tag="LP524_001143"
     CDS             6..305
                     /locus_tag="LP524_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2234208.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="LP524_001144"
     CDS             378..1739
                     /locus_tag="LP524_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBL2234209.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="LP524_001145"
     CDS             1761..2021
                     /locus_tag="LP524_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234210.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="LP524_001146"
     CDS             2035..2787
                     /locus_tag="LP524_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBL2234211.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="LP524_001147"
     CDS             complement(2881..3486)
                     /locus_tag="LP524_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL2234212.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="LP524_001148"
     CDS             complement(3539..4513)
                     /locus_tag="LP524_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HBL2234213.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="LP524_001149"
     CDS             complement(4891..5610)
                     /locus_tag="LP524_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL2234214.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="LP524_001150"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="LP524_001150"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBL2234215.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="LP524_001151"
     CDS             7161..8558
                     /locus_tag="LP524_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL2234216.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="LP524_001152"
     CDS             8639..9079
                     /locus_tag="LP524_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBL2234217.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="LP524_001153"
     CDS             9227..9553
                     /locus_tag="LP524_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL2234218.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="LP524_001154"
     CDS             9554..10303
                     /locus_tag="LP524_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234219.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="LP524_001155"
     CDS             complement(10383..10838)
                     /locus_tag="LP524_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBL2234220.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="LP524_001156"
     CDS             complement(11265..11540)
                     /locus_tag="LP524_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234221.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="LP524_001157"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="LP524_001157"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBL2234222.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="LP524_001158"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="LP524_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBL2234223.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="LP524_001159"
     CDS             14770..17436
                     /locus_tag="LP524_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBL2234224.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="LP524_001160"
     CDS             17800..18552
                     /locus_tag="LP524_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL2234225.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="LP524_001161"
     CDS             complement(18574..19728)
                     /locus_tag="LP524_001161"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HBL2234226.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            19913..22354
                     /locus_tag="LP524_001162"
     CDS             19913..22354
                     /locus_tag="LP524_001162"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HBL2234227.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(22684..23388)
                     /locus_tag="LP524_001163"
     CDS             complement(22684..23388)
                     /locus_tag="LP524_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234228.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(23575..23820)
                     /locus_tag="LP524_001164"
     CDS             complement(23575..23820)
                     /locus_tag="LP524_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234229.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(23837..24688)
                     /locus_tag="LP524_001165"
     CDS             complement(23837..24688)
                     /locus_tag="LP524_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBL2234230.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            24854..25858
                     /locus_tag="LP524_001166"
     CDS             24854..25858
                     /locus_tag="LP524_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBL2234231.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(26099..26455)
                     /locus_tag="LP524_001167"
     CDS             complement(26099..26455)
                     /locus_tag="LP524_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBL2234232.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            26823..28010
                     /gene="tuf"
                     /locus_tag="LP524_001168"
     CDS             26823..28010
                     /gene="tuf"
                     /locus_tag="LP524_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBL2234233.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(28198..30789)
                     /gene="mprF"
                     /locus_tag="LP524_001169"
     CDS             complement(28198..30789)
                     /gene="mprF"
                     /locus_tag="LP524_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBL2234234.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(30815..31576)
                     /locus_tag="LP524_001170"
     CDS             complement(30815..31576)
                     /locus_tag="LP524_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL2234235.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            31854..32375
                     /locus_tag="LP524_001171"
     CDS             31854..32375
                     /locus_tag="LP524_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HBL2234236.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            32434..33294
                     /locus_tag="LP524_001172"
     CDS             32434..33294
                     /locus_tag="LP524_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HBL2234237.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            33479..35794
                     /locus_tag="LP524_001173"
     CDS             33479..35794
                     /locus_tag="LP524_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBL2234238.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            35976..37817
                     /locus_tag="LP524_001174"
     CDS             35976..37817
                     /locus_tag="LP524_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBL2234239.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            37841..38950
                     /gene="rpoD"
                     /locus_tag="LP524_001175"
     CDS             37841..38950
                     /gene="rpoD"
                     /locus_tag="LP524_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBL2234240.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
BASE COUNT        12550 a         6865 c         8086 g        11507 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagccg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat tgttaaaatt taatcttaga aaaaaataac tggaacaaga
    19861 gctatttgtt tttacagcaa agaatggtat atttttgtag gaggtggctt acttgaaaga
    19921 agtcgttatg atagatgctg caagaacgcc gataggaaag tatcgcggaa gtttaagtcc
    19981 atttactgca gttgaattag gcacacttgt gactaaagga ttattagata aaacaaaact
    20041 gaaaaaagat aaaatcgatc aagtaatatt tggaaatgtc ctgcaagctg gtaatggtca
    20101 aaatgttgcc agacagattg ctttgaacag cggtctccct gtagatgtcc cagcaatgac
    20161 gatcaatgaa gtttgcggat ctggtatgaa agcagtgatc ttagcacgcc agctgatcca
    20221 attaggtgaa gcagaacttg tgatagccgg cggaacagag agtatgtctc aagcacctat
    20281 gctgaaacag tatcagtcag aaacaaatga atatggtgaa ccaatttcca gtatggtcaa
    20341 cgacggattg actgacgcat tttcaaatgc acatatggga ttaaccgcag agaatgttgc
    20401 aacacaattt tctgtgagca gagaagagca ggatcgctat gccttgtcgt cccagttgaa
    20461 agcagcacat gctgtcgaag ccggtgtatt ttctgaggag atcatcccag tcaagatttc
    20521 tgatgaagac gtgttatctg aggatgaagc agttcgtgga aatagtacat tggaaaaact
    20581 gggcacgtta cgtacagtat tctcagaaga aggaactgta acagcaggaa atgcttcccc
    20641 gttgaatgac ggtgcctctg tggtgatcct tgcatccaaa gaatacgcag aaaataataa
    20701 tctgccttat ttagcaacca tcaaagaagt agcggaggtc ggtattgacc caagtatcat
    20761 gggaatcgct ccaatcaaag ccattcaaaa attgacagat agatcaggaa tgaatttatc
    20821 aacaatcgat ctttttgaga tcaatgaagc atttgcagct tcttctatcg tagttagcca
    20881 agaactgcag ttagatgaag aaaaggtcaa tatatatgga ggagcaatcg cattaggcca
    20941 tccaattggt gcaagcggtg cacgtatctt gactacatta gcttacggat tgcttcgtga
    21001 acagaagaga tacggtattg cttcattatg tattggtgga ggtttaggct tggcagtctt
    21061 gttagaagcg aatatggagc aaacacataa ggatgtgcaa aaaaaaaagt tttatcagct
    21121 tacgccatct gaacggcgca gtcaattaat agaaaaaaat gtattgacac aagaaactgc
    21181 gttgattttt caggaacaaa cactttctga agagctctcc gatcacatga tcgaaaacca
    21241 agtgagtgaa gtggaaatcc caatggggat cgcgcagaat tttcaaatca atggcaagaa
    21301 aaaatggatt ccgatggcaa cagaggaacc atctgtcatt gcagctgcaa gtaatggtgc
    21361 aaaaatctgt ggaaatatct gcgcagaaac acctcagcgg ctgatgcgag ggcaaatcgt
    21421 tctatcagga aaatcggagt atcaagcagt gatcaatgca gttaatcatc gaaaagaaga
    21481 gctgattctt tgtgcaaatg aaagttatcc ttcaatcgtg aaacgcgggg gcggtgttca
    21541 agatatctct actcgggaat tcatggggag tttccatgca tacttatcta ttgatttttt
    21601 agtggatgta aaagatgcaa tgggcgctaa catgatcaac tcgattcttg agagtgtggc
    21661 aaacaagtta cgggagtggt tccctgaaga agagattcta ttcagtattt taagtaattt
    21721 tgctacagaa tcattggctt ctgcctgttg tgaaatccca ttcgaacgct tagggagaaa
    21781 caaagaaatc ggtgaacaaa ttgccaaaaa aatccaacag gcaggggaat atgcgaagct
    21841 tgacccttac cgtgcagcaa cgcacaacaa agggatcatg aatggtatag aagctgtagt
    21901 ggcagctaca ggaaatgata cacgtgcggt tagcgcttcg atccacgcct acgcggcaag
    21961 aaatggatta taccaaggat taacagactg gcagatcaaa ggggataaac tagttggaaa
    22021 actaacggtg cctttggctg ttgccacagt aggcggagct tccaatatcc tgccaaaagc
    22081 aaaagcatct ttagctatgt tagatattga ttcagccaag gaactagcac aagtaattgc
    22141 tgcagtaggg ttagctcaaa acctggctgc tttgcgtgca ttagttacag aaggaatcca
    22201 aaaaggccac atgggacttc aagctcgttc attagcgatc tcaattgggg ctattggtga
    22261 ggaaatcgag caagttgcga aaaaattacg tgaagcagaa aagatgaacc agcagacagc
    22321 tatacaaata ttggaaaaaa tccgggaaaa ataaaaaagc aatatcttta gaagaataaa
    22381 gcaagattta cagttatttt atttactgca gatcttgctt ttttctctat cagaaaatat
    22441 gtttattatt agaatttttt atagttaagg ttaggcatct taagaaatca ggcaactcca
    22501 agatatcttc aggtagaaga gaagtcgcct aacattgtag gatcataggt gatttgttgg
    22561 acaggtcaag ctgatatttg agcctgatgt tagaaggaat ttctttttac ttgctgtaga
    22621 aattattata tgactaaaaa ggctgggatt ttaaatccca gccttttatg acaaatagaa
    22681 tttttatcga tcagtaatcg ctgtcgctgc aaattgttcg atcaaaggaa tcaaagtcaa
    22741 tttgactttg tatttccgtt cgagtgcttt ttcgataacg aactcatttt cagtagtcat
    22801 agaaatagtt gctaatttac gaggttcatc agcatttttt tcattaataa ctacttcatg
    22861 ttcattttgt tctgtgtaaa atttaggata ctcagcaacg gcttttgaaa aatcagcaaa
    22921 acttaaagcg gcattcgtat cagttgagcg tgtattttca gttgtgtccg cttttggttt
    22981 ttccgttttt tcttctggca cactgactgt ttcttctgta actggtgtcg caacagcttg
    23041 cgcagttggt tcatcgtttt tcaatcgttg atcattccag taagacacga tttcaataag
    23101 aagattgtca cggaatcgtt cgaaaatccc gttttctcct gaacgaattc tctttaatac
    23161 agtattgaag ttgtcatcga ttggcacgtt gatccgtgta tcttcatttg ttttcccagc
    23221 taatccgatc gttaagattt gacggttttt gggatcaagc atgacttgta acggcaaacc
    23281 ataacgtcgc aagctctctt cttctagaga acggaataat tgtcctctta caccatccaa
    23341 tgaaggacgg aattgattta ataatttttg tttctgtttt ttcttcatat gttctctcct
    23401 tcgctcatac cctttcgatt ataagaaaaa aacagccaaa tgaaaaggag taatgcaaaa
    23461 aaacttgcgt atacgcaaat tacaacagaa aaatttgcgt attttcccgt tttttgaaag
    23521 aaaaataaaa aatggaacgg attatgtata tatacataat ccgttccatt ctttctattt
    23581 tttcggtatc aaggcaaaaa caggtagttc aggtgaataa atgacttcat gttcgtgcca
    23641 gggtttgacc attagcgaat gatcgaaccg ttttttcagt tcttcgatga tttgatcatg
    23701 tgtccagttt cttgcaggaa aatgttggat tttctctgca tcgatcaaaa agacaaaatc
    23761 ggaatgaaaa gtctgggtaa cttcttccga gagagaattc aagcgttcga ttggatccat
    23821 aaaatcacct ttcgacttaa aattgggtag tatctgggtc ttttgctttg ccgactacac
    23881 catctaactg atcgatttgt ttcatttctt cttctgttaa ttcaaaatca aataattcag
    23941 tattttcttt gattctgctt ggtgtcactg acttaggaag aggaaggaat tcgtgttgca
    24001 gcgaccaacg caaagcaact tgtgcaatcg tcttgtcatg tgcttcagcg atttgtttca
    24061 tttcaggcac atcgaagatt ttaccagtgc ctaatggact atatgcttcg aggatcatat
    24121 catgtttttt tgcatactca actacagttg tctgtaattc gcctggtgca aggaaaactt
    24181 gattgaccat cggcataatt tttgctgttt ttgctagtgt atctagatgg tggggtaaga
    24241 aattgctcac accgattgct ttgattttac ctgcttcata cagttcttca aatgctcgcc
    24301 atgtatctgc atttgcttgt tcccaattat cacgaaaagc aacagggttt ggccagtgga
    24361 tcaaaaacag atctaaataa tctgtttgga gcttcttcaa tgattcttca aaagaagaca
    24421 tcacaagttc atagctgtgg tttgcattcc ataattttgt tgtcaagaat atctcttctc
    24481 taggaattcc actttcctta atcgcttggc cgacgctttc ttcattcttg tatccttggg
    24541 cagtatcgat atggcgataa cctgcagcca gcgcttcttt aacagaggaa acagcgacat
    24601 ccccatcagg tgtttgccat gtgccgaatc ctactaccgg gatttcgtaa ccatttgata
    24661 gacgataagt gcttgttaat gaattcatac tttcagctcc ttttttattc gttcaactat
    24721 aagtgtagcg catttttcga aaaaaagaat ccattttgtt tctactcttg aaatttttgt
    24781 ttaaaagaaa atgattttga ttctggtatt tttgattttt tttccgtaaa ctaagacaga
    24841 agggaatgag aaaatggata tctatttaaa aaaagcaatt ctgcatatta ttgacagaga
    24901 aactggttct ccagtctttt cacaaaaaga gctggatctg acaaaagaat atattcgtga
    24961 ttttcttcaa aagaagatcc aaaaaatctc ttctgctcaa acaaaaactg gacaactagc
    25021 agaagaaagc atgatcgttg aatcacttaa aatcgctcaa gaaaattttg tggaagcaag
    25081 tgaagcaatc gttcagcggt ggttcgatat ctaccaagaa agtgaagaag cacctagtgc
    25141 agatgtattt gttgtcttgt atgaattaga tacagtgatg tacttatctt tattgaaagt
    25201 caattatcgt gaggcttaca ctcactttgt tgaagcagac gaagcaggga tcgacaaccg
    25261 cttgattttg aatcgagcaa ttctaggggc gaaaacacaa aaagcagatg aagcattcgc
    25321 tgtgaatttg actgattgga cctacgaatt aattgaaaaa aaatatgagt tttctggcaa
    25381 aaaagagctt tatttttctg gaagagtgat cgaaagtgtt cctgcacctt ctttagaaga
    25441 aaatgtaaaa gtgatccaaa aagtagccaa gcatcttgga aaaaaatttg agacagaaga
    25501 atttgatatc atggctgatt tgaaagaagc tgtctatgat acgatagaag aaaaaggtcg
    25561 gttggatcat gagatgattg cggaaaaagt cttcaaggaa aacatcacgg caaaaatggc
    25621 ttttaaagaa gaagtacaag agcaaggatt tgtcccagaa gcacctcctg taaaagaagt
    25681 acaagaaatt tctgagaaaa aatttggaaa acaaaaattc aagctatcaa acgggatcga
    25741 actgatcgta ccagtagatg tatatcgcaa cccagatttg attgaatttg tgaataatcc
    25801 ggatggtacg atatccgtga tgatcaaaaa tgtggatgaa gtattgaatc gtttataaaa
    25861 atgactagaa tattaggtgt tcaagtacca gtgatcagtt aaatgaattg tgtcaagaga
    25921 aggatcggaa aagacgaata taacaaaaaa agagcgtgag acaaaagtaa agaacacttt
    25981 tgtctcacgc tctaaacacg tataaacggc ggaagcagaa gcaactcctt cttatttctt
    26041 ggagttaaac gcttctgtcc cagcctcttt ttatctaata aaattttaag accatttact
    26101 aattatcgat tttttctgcc gcatttttag gcacttcaga agcctttatt tcttcccagc
    26161 ttaatacacc tttacgagga ttgactttga gtttaagata cgcattcatc tttaatggat
    26221 gttctctttc ttcgcgaagc gtcacctgtt ttttttcacc atctttatta taagccgctt
    26281 gcttgtaggt ataagtcgtg aatttctctc cgttatctgc ttgggtagct gacatttcgc
    26341 cattcgtcgt gatttttgta taataagctt cgccgccgta aaaataatga taagctgcaa
    26401 aacttccgcc gagaaatatg cctaaaacga caagtacgcc aattaatttt ttcatgtact
    26461 tcacctcttt ccttattatt aacgaaatag aagatttcgt ctattgaaag ttgttggcaa
    26521 cagctgaatt ttaaactttt cctcattttg ttcttattgc ttcaaaagaa agttaaggac
    26581 tatcttgaag tttttctcaa aaaacacatt tttttattta taaaaggtac ttttttcgcg
    26641 tataagacag acgagtgctc ttagttgact ttgaccaata ttgacgtata ataagcttag
    26701 ttcgaatctt acaaaaagta agtcggacgt attctttttg atttagtcaa aaaaagacta
    26761 taataaaaag gtgagagctg aaatttttca gtgatctata ttatttagga ggtttgtaaa
    26821 ttatggcaaa agaacattat gacagaagta aaccacacgt taacattggt acgattggac
    26881 acgttgacca tgggaaaact actttaactg ctgcgatcac tacagtatta ggcaaaaaag
    26941 gcctagcaaa tcctcaagac tatgctagta ttgatgcagc tccagaagaa cgtgaacgtg
    27001 ggattacgat caataccgca cacgtagaat atgaaacaga aaaacgccac tacgcgcata
    27061 ttgatgcacc aggacacgcg gactatgtga aaaacatgat tacaggggca gcacaaatgg
    27121 atggtgcaat cttagttgtt tctgcaactg acggtccgat gcctcaaaca cgtgaacaca
    27181 ttttattgtc acgccaagtt ggtgtaaaat acctgattgt tttcttgaac aaagttgatt
    27241 tagtcgatga tgaagaattg atcgatttgg tagaaatgga agttcgcgag ttattgagcg
    27301 aatatggttt cccaggcgat gacactcctg tgatcaaagg ttccgcatta aaagcattgc
    27361 aaggcgatcc agatgctgaa gctgctatta tggaattgat ggatacagta gatgaatata
    27421 tcccaacacc agaacgtgat acagataaac cattactatt gccagtggaa gacgtcttct
    27481 caattacagg tcgaggaact gttgcctcag gtcgtattga tcgtggtgct gttcgtgtcg
    27541 gtgatgaggt agagatcgta gggatcaaac ctgaaacaca aaaagcagtt gtaacaggtg
    27601 tagaaatgtt ccgtaaaacg ttagattacg gggaagctgg ggataacgta ggcgtgttgt
    27661 tacgggggat ccaacgtgac gatatcgaac gtggacaagt acttgctaaa ccaggttcca
    27721 ttactccaca tacaaaattc aaagcagaag tgtacgtgtt gacaaaagaa gaaggtggac
    27781 gtcatactcc attcttcaac aactatcgtc cacagttcta cttccgcaca actgatgtta
    27841 caggaacaat cacattgcca gaagatacag aaatggtcat gcctggtgac aacgtgacga
    27901 ttgatgttga cttgatccat ccaatcgctg ttgaaaacgg aacgacattc tcaattcgtg
    27961 aaggcggacg gacagtaggt tcaggtatcg ttacagagat cgaagcataa gaaacgcgac
    28021 tcgaatattt tggtaatgaa aaatgtttaa aataaaagag cggtccaaac aggatttctg
    28081 tttggaccgc tctttgttct tcaattgtat aagaagagat tgtagaagca tgaactttcg
    28141 ttttacttca gtctctttta ttcatcatga gagtgttcat ctatcggtat gcagttttta
    28201 atactttctt cgtatccatt ttttcaaacc acttattcta ccttgtgtct gctcaactgg
    28261 cgcattatca ataatcagca aagcaatcat aacatatgca atccaactat ctctagaata
    28321 tagagtataa cgtgagaccc agtttgtagc gtacttgctt ttatattctc ttaatccatg
    28381 gaaagaataa aaatgagaac caaactcgta aaccaagtat gcaatccttt cctgtaaaaa
    28441 actctttctg gaagttccga catttgaaag aggggccatc cctaaattga accattggat
    28501 atgttcttct ttcatatagg caaacaaatg caagaacaag aaatccatac tgccagaagg
    28561 tgctttttta gaatcatatc gcatcagatc aatcgttcct actttattgt tcgtgtaagt
    28621 aggcatgatc gttgcaaaag cgacaatttc ttcttgggta tttttgacaa ctgcaatagg
    28681 cgctctttgt agataatctt cagagaaaaa gcctaaagaa aacccttttt cctttcgtga
    28741 acccaaccaa ttatcagaga tatttttgaa agtactcatt tgctcagtag aaaatggtgg
    28801 ttgtaagaca tcaaatgtaa atccttctcg ttcgatccga ttcaagacag cacgagtgcc
    28861 cttcattttt ttccctgaag ttgtaaaact attcaggtct acataagctt cttcccccat
    28921 cttaatgaaa tcgtagccga attcatgcaa aatcataaca atctcttcgc ttgtttcgta
    28981 gaaaactggt aaatagcata gtcgatctgt ttcttcgata aaagcttcga ttgcttctgg
    29041 aaaatcttct ttcttaccag aaggatcacc cattacaata catttattat tgaagcaagc
    29101 gaattggaga agaacagttg gttcaccgtc tttttcatag gcaaacatcc gcttatcttt
    29161 taagaagatc aattgactat cggagtttcc tccataagtt gtaaggatct tcagggcctt
    29221 ttcttcatta aaggcttcgc caatctgttt cttttctcct tgaaggaaat gaacaaatag
    29281 aacgatcatg aaactaacag cgatgatggc caacaatccc gaaaaccaaa tcttttcaga
    29341 aggaaataag aaaaaggaaa taaaatggtg acgccgatga gggaaatccg gcagattgta
    29401 taccccaata acgatgtaaa gcaaggacag tgcaccaata atgattccat cgatcgtacg
    29461 ccactcccat gaatagatca attgttcccg aaataattcg tttttagaag caagaatgat
    29521 cagaagcaga atagacaaga aaaccaccgg tgtgaaacta aaatcactta ataaaacata
    29581 aagtaaagct agaacaatca agaagattgt aggcaaatat gctcgtttca cacgtgctgc
    29641 gatccctctg cccataatga ttaaaagaaa tcccaatagt atactaggaa attggataat
    29701 aaaatgaaat ttcaatgggt tcaaactatg aagccagcga aactctgtaa aagcttgcgg
    29761 gatagtggca gaaagtacta gcatgattcc agaaaagtat aataagacaa ccacgatttt
    29821 atgcgcaatt tctgttgcta attgtttagg gataccagaa taacgctgat caaaagtgga
    29881 accgatattt ttgaagaaaa agacgattcc aatcaaaaac ggaatgatgt agtagaacag
    29941 gcgatagagc aagatccaca caacgactat ttctcttggc acaccaatag cggacaaccc
    30001 gataatcatc attacatcga aactcccaat ttctcctgga atcatagaca cgattcctat
    30061 cactgaagca gcaatgaata atggaattgt ttgccacaga tcaatccgga tttccattaa
    30121 ataaccaaca gaaaggaaac taaacaggac accggtccat tccaacaatg aaactagcaa
    30181 tagctgtcct tgagttttaa gtgaggtacc accaaggaat cctttctttt tgaaagtagt
    30241 aaataaaaag acgacaggaa aatatagacc gccgccgatc aaccatatcc aatattgctt
    30301 caaaaaagga gcaactggcg tgaagactac aagtaaaaaa gaaagcagac tgtaaataga
    30361 aagtccagcc atcaaaaaca agaaaacttt cgataatgct tggatcacgt ttctgctctc
    30421 tttcttttgt ccatatagtt cagagcgaag ccccacacta acaaacccgc cgaaaccggc
    30481 aatattgttg atcgtattga tcagccagct tgtctcaaac aggtaacgag gattctgctt
    30541 ttgttcaagc atccgattca aaataatatc ataaccaacc ataggtaaca cagagataag
    30601 accaatcaat agcattagtc cgcttttcca tacaggtata gtggcaaacg tttctcctaa
    30661 ttgctggata gatagagttt taccgatgga catcaattca ccgacaataa tgacaatgac
    30721 agaaatcaaa aagattgttt tgaacagtcc taggtggttt ttcagccatt gtatggtatt
    30781 ttttaacaaa taaatcacct tctattccta atagttatgt ttcagaaaga aattgaagaa
    30841 cttctcgatt gaagcgttca ggatcttttc tagctaattc atgtccttgt tctttcacaa
    30901 gcacaaagga agcttttggt atagttttag ctatatacaa agaatgtttt agcttgatta
    30961 catctttttt tcccacgatg atcaaagtgg gggagttgat tttttttaaa tcattttctg
    31021 taagcccgat gtcatgcagc aaaagtttga taactaaaag atttttgcga agacttgggc
    31081 gaaataaaga gagtaaccat acccatgcat aatgaatatt actgatgact ctggcagaaa
    31141 aacgaacgcc tttaacaagc gtgtttccag aatttaaaat caatcgatga acttttttag
    31201 ggaagctgct ggcaaatacc agtgctagat ttgctccatc actaaatcca agaaaatctg
    31261 cctgatcgat tttttccagc aacatgatcg tattcagatc ctcggccatt aactgaaaat
    31321 tcaacataga tgcttcgttt gtcgaacgac catgtcctcg gctatctact agatacacgg
    31381 tatagtagcg ttcgagtaca gggacctgtt ctgaaaaaaa gcggccgctg ccgtcattgc
    31441 catgcagtaa aaagagaggg aagccttgac cgctcttttc atagtaaatt tcgctcccat
    31501 ctggcatttt tgcatatttc ttttcttttt tcacataatc acctactgtg taaagtataa
    31561 cgattatatc agtcattggg caattgtttt tactgaaaaa caacgatcat tttcgtaaga
    31621 tgtcaaaaaa tacaggaaaa ttcatttttt cttaaggcgt acacagaaat caaggaagaa
    31681 atagccaaga agataaagaa atagtaagat aaaaaaagat gtcaaaataa ctaggtgatg
    31741 aaatgaagcg aaaacgatta tctattatcc gtattctttt ttacatgatc attctgacgg
    31801 ttattggtgc ggtgttgttt ttatgaggaa gaacttatcg ttcggtaaaa cgagtggaaa
    31861 gctatcaaat agaaataaca acagctgcaa aaaattatca tatggaaaac tacgagaatt
    31921 tgatcaaggc gataatattg accgaatcaa aaggaaaagg gacagatctc atgcagagct
    31981 cggaaagtct gcatggagaa gctgagatca ttgataatcc agctgacagt attgatcaag
    32041 gtgtcaaata tttggcggaa atgatagtag aagcaaagaa acaaggatgc gatttatgga
    32101 cagctgtcca agcatataat tttgggaaag attatattgc ctatgtaaaa aatcatggcg
    32161 gagaaaacac agtagaatta gcagaagcgt attctaaaga tgtgctgagc ccacttttag
    32221 gaaatgagga agggaaacaa taccgatatt ggcggatcca gtccttgctt tacaatggcg
    32281 gctatctcta tcataatgga ggtaacatgt tttatcctga tattgtcaaa atgaatgaaa
    32341 aaataataga gacctatgaa aaagtcttta agtaaaatag caggaaatgt tcaatcatga
    32401 ataaaattaa atgaaagaag gagtgcgcaa atagtggaaa aaaggcaaaa aatggaaaat
    32461 caagaagtgg ggtacacaac caaagactgg atgttacggt ttgtgaaggg aatgtttatc
    32521 ggttcaggat ttatcttgcc aggagtcagc ggtggtgcat tagcagctgt ctttggcatg
    32581 tatgagcggc tgatttcttt tttagcccac cttacaaaaa atttcaaaga aaatgttttg
    32641 ttttttctgc ctgttggtct aggcggagtg acaggcatct ttctactttc ttttttagtc
    32701 agttttttat taggtgcgta tgaaacaacg atattatggt tttttgtcgg atgtatcgct
    32761 ggaactgttc ctgcgttatg gaaagaagca gggaaaaaag gaagagatac ttctgatgtg
    32821 atcatcatgg ttgttacttt tgttctggct tatttgtttt tattatacgg cgctcaatta
    32881 tttgacgggc aagtcgatca gaattttgga acatggctga ttgccggcgg attgattggt
    32941 ttgggtatga tcgtcccagg attgagccct tctaattttt tggtgtatat gggattgtat
    33001 aaagcgatgg ctgacggatt taaagaagta cgacttgaag tgattgtgcc gatcggaatt
    33061 ggtgggattc tttgtgttct tggattgtca aaaatcatgg attacatttt ctcaaaggca
    33121 tatagcaagc tattccattt cattttaggt gtagtttttg cttcaacgat tatgatcatt
    33181 cctaccgatt acgcgggatt gggagttgct ggggtactag cttgtcctgt tctttttata
    33241 gccggtacag cactaggttg gtggatgagc cgtttggagg aacaatataa gtaaagatag
    33301 attgataata aaaacagcac acgggtgatt caaacaaaaa ggttggcatc ttttttgtaa
    33361 aatcattcgt gtgtttttct ataaatttta taaaaacaag ttgtttttaa tcttggaaca
    33421 cgctagactt aaacacgtga ttatttttga aagttggcgg aaaatggaga aaaagaaaat
    33481 gaatggactt acacagcaag aggtagttca gaaaatggct tctggccagc aaaatgacta
    33541 ccaggaagat gcatcaaaaa gtacaaaaca aatattcagc gataatatat tgacgttatt
    33601 caattttctg aactttggta ttgggatttg tctgttattt gtaggtgcgt attccaacat
    33661 ggcgtattta gcaattattg tcgtcaatat cgttattgga atttatcaag aaatacatgc
    33721 aagaaatctt gtacggaaat tatcgattgt ttcccaatca gaagtactag tgatccggga
    33781 cggtaaagaa caatcgattt ctactaaaga gctggtatta ggagatttgg taaaaatcgg
    33841 agcaggagaa cagataccag ccgatttaca ggttatatct ggacgggcag aagcaaatga
    33901 agctcttttg acaggcgaat ctgatttgat tgtcaaagaa actggagctg aattgctttc
    33961 tggaagcttt ttgacaagcg gttctgtcat ggctgaagtt atccgagtag gtgcagataa
    34021 ttatgctgtc cgcttgacta gcgaggcaaa gactcacaaa gctattcgtt ctgaacttgt
    34081 agattcgatt cgcaaagtat ccaaattcac tagtttcgtg attattcctt tggggatcat
    34141 actatttttg gaagcactgt tcatccgcga tgcttctatc aaaatttctg ttattgcatc
    34201 tgcagcagct ttgttaggca tgcttccaaa gggtttggta ttgctaataa gtattgcatt
    34261 aacgacaggg gtaaccaaac tggcaaaaaa acgaatcctt gttcaagaca tgtattcaat
    34321 tgaaacgctg gcccatgtcg atacattatg cttggataaa acagggacca ttactgaagg
    34381 gaaaatgagt atccagcggg tagaagcact acatccgctg tacgagaaag ccatcccaga
    34441 aattatgggg acgtatctgg cagaaagcag tgataacaac atcactatgc gggctatgag
    34501 agattatttt gagctttcta atcggtatga tgtgacaaac aaagtcgcat tttcttctga
    34561 tcgcaaatgg ggcgcgatgg aatttccaga actgggagtc gtgtacgtag gagcgcctga
    34621 acgtttggtg gaagaagatc ggcttccgga cagtctaaaa acagcccaag cgaatggcta
    34681 tcgcgtactt atgttgggga tcgctccaaa tcagcaattg aacgaagaaa aaccagcatt
    34741 tgttgagccg gttgctctgt ttgaaatcga tgatccaatc cgaaaaaacg ctcaagaaac
    34801 actagcttat cttcatgagg aaggtgtgga tctgaaagtg atttctggag acaatccttt
    34861 tactgtctcc aatatcgcta gacgtgcagg attaccgaat tatgatgcgt atgtcgatct
    34921 ttctcaaatt actgaagaaa tggaagtgcg agaagctgct catcgctata cggtttttgg
    34981 acgagtgagt ccgcagcaaa agaaattgct ggtaaatgaa ttgaaagaaa gcggcagaac
    35041 ggttgcaatg acaggcgacg gtgtgaatga tgttttggcg ttaagagaag cagattgcag
    35101 cattgctatg gcagaaggtg atggagcaac acgtcagatt gcgaatctgg tattgttgga
    35161 ctctgatttt actacattgc cagaagtcct gtttgaagga cgccgagtgg tcaacaatgt
    35221 gacgaaagtc tcaggcatct tttttatcaa aacgatttat tcatttattt tatcgattat
    35281 atgtgccgtt acagcaattg gatttccgtt tatccctatc caaatcacgc tgattgattt
    35341 agcgattgaa gggtatcctt ccttcttttt atcttttgaa caagataaaa gaaaaatcac
    35401 ttaccgttat ttgcctacag cacttattaa tgctttacca aatgccttgc ttgtagtagt
    35461 gaatatcatt gcggtgtatc tacttggaaa aacattcgca tggggaaatg ctgaaactac
    35521 tactttgatg tattacttgc tgattgggat cagttgcatg gcagttgtaa aagcttgtta
    35581 tcccttcaac ccattgcgca tcttcctagc agtaacgacg atcgtaggga tatatgtagc
    35641 tgcaatgctg ttccatggaa ttttggaagt aaacttaggg ttgggagaga gcgtaggata
    35701 cttctttgta ttcatgctcg taaatattgt tcttcgagta ttgtactgga aggctgatat
    35761 tcaaaatcag ttactgaaaa gagtagcaaa ttgatgctta atgagataga aatgtgttat
    35821 acttgaaaaa gcaatgaaat atgatacaat caaacatgta taattgtgtc ctttaaaatc
    35881 gcactgattt cagttagtgc gattttttac cggttattct cctgaaatat cttctgttaa
    35941 acccaaaaga tatcaataga gcagggggtg aaaaaatggc tcaacggatt cctcaagagg
    36001 ttatcgaaac agtacgcagt caaacgaata tcgttgaggt gattggacaa tatgtccagt
    36061 tgaaaaagtc tggtaaaaat tacttggggc tgtgtccttt tcatgaagaa cgtacaccgt
    36121 ctttttctgt tgcagaagac aagcagattt ttcattgctt tggttgtggt aaaggcggaa
    36181 acgtgtttac ttttcttcaa gaactagaag gtctaagttt tccagaagca gtggtaaaag
    36241 tggcagactt cgaacagata ccgttagaag atcaatggcg tcagcgtact atagctgtcc
    36301 aaaatcctga atctgctacg gggaaactga ttgctgctca tgaaaaagca gcggaaatct
    36361 atcatcacat gctattgcat acaaaagctg gacagccagc tttagaatat ttgcaaagtc
    36421 gtgggttgtc tttagaatta attgaagaat tcaacatagg ttttgcacct aatgagcgtt
    36481 cttttcttca gcaagtattc aagaatgaat caatttcaaa tgaattgtta gaacgctctg
    36541 gattgtttgt cacgcatgat gatggccgta tgtctgatcg tttttatcaa agagtgatgt
    36601 ttccacttcg taattctcaa ggccagacga tcggtttttc aggacgctgg cttgcacctg
    36661 aaaaagggca ggaaaaagat cagcccaaat acttgaatag tccagaaaca gaactgttca
    36721 ataaaagaca agttttattt aatcttgata aagcaagaag cgatatccgg aaaaacggag
    36781 aaattttgtt gttcgaaggg tttatggatg tgattgctgc atggcaggct ggtatcaaaa
    36841 ctggattagc ttctatgggg acaagtctaa cagctcaaca gatccaacaa atggaaaagc
    36901 tgacgaaaga attggtcttt tgctatgacg gagacaacgc aggttttgaa gcaaccaatc
    36961 gagggattga attgcttcgg caaaacagtc gattagcact ttctgtcgta gtcgttccag
    37021 aaaagcttga tcctgatgaa tatctccgta agtatggaga agcatctttc caagaattag
    37081 tggctcatgg gcgcgaaaca gtctttactt ttaaatcacg ttatttaaaa caagggaaaa
    37141 atttagataa tgaaaaagac aagatcgaat acctcgaaga attactagtt gaacttagcc
    37201 gagttgtttc accaatcgaa caagatatgt atctatctca actgtcaaca gagtttcaga
    37261 tcggcagaga gacgatacaa aaacaactgc gcgatcttcg aaggacaaat cagcaaaatc
    37321 gtcaagaacc tcaggaaagt caacacactc aggttacgag acaagaaact gcacggaaaa
    37381 agagaccttt gacgcaagta gaaaaggcag agcagattct attatatcgt gcatttaaag
    37441 aagtttctgt ccgtaatatt ttaaaagaac gagaatttca gtttattcat gatgcatatg
    37501 cagaagttta ttttttattc gatgcatatg tctcacaaca ttatgaattc aaccttgcag
    37561 agtttttaga ctttttgcaa gatgaaaatt taaggcggtt ggtggtggaa atcgaatatt
    37621 taccggtggc agaagaaagt tcgccacgag aaatccaaga cttgctagat gtgatcagca
    37681 aatctggctt agctgatgaa ataacattaa aaaaacaaat gcagcaagaa gccagacgaa
    37741 ctggaaacaa acagcttgag cttgaattga cgattgaaat catcaattta acgaaacagt
    37801 taaaacaagc caaatagcta ctcaaggggg gccttctttt atggcaaatg gaacagataa
    37861 aaaaacgtat gaagcagcag tagcagcttt catcaaagaa aacaaaccca aacgacaagt
    37921 tgtatatgat gatttatcca atcagcttgc tacaccattc actttgaatg cagatgaaat
    37981 ggacaaattg atccaaaaga ttgaagacgc aggtatcagt gtggtggatg aaaatggtga
    38041 tccatcaatt cacagcttga agagtgctga gaaaaaagct gaacaagcaa aaacagaaga
    38101 tctttctgcg ccaaccggtg taaaaatcaa tgacccggtt cgtatgtatc tgaaagaaat
    38161 tggccgtgtc tcccttttaa ctgcggaaga agaagtagcg ttagcattga aaatcgaaga
    38221 aggcgaccaa gaagcgaaac agcgtctggc agaagcaaac cttcgtttag tagtaagtat
    38281 cgctaaacgt tatgtaggac gaggcatgca gttccttgac ttgatccaag aaggaaacat
    38341 gggactgatg aaggcagttg aaaaatttga ctaccgcaaa ggatttaagt tttcaaccta
    38401 tgctacatgg tggattcgtc aagccatcac tcgagcaatc gctgaccaag cacgtacgat
    38461 ccgtattcca gttcatatgg ttgaaacaat taataaattg atacgtatcc aacgtcaatt
    38521 gctgcaagac cttggaagag aacctacgcc agaagaaatc ggtgcggaaa tggatcttcc
    38581 aacagaaaaa gtccgagaaa tcttgaaaat cgcgcaagaa cctgtttccc ttgaaacgcc
    38641 aatcggtgaa gaagatgact ctcacttagg tgatttcata gaagaccagg aagcaacaag
    38701 tccagcagaa cacgcagctt atgagttact aaaagaacaa ttggaagatg tactagatac
    38761 attgactgat cgggaagaga atgtccttcg cttacgtttt ggtttagatg atggccggac
    38821 gagaacattg gaagaagtag ggaaagtatt tggtgttact cgggaacgta ttcgtcagat
    38881 cgaggctaaa gcattaagaa aattgcgcca tccttctcgt tcaaaacaat taaaagattt
    38941 cttagaataa gcgtggacta ttgaccagaa gagaaatcag taaaactgtt tggtttattt
    39001 taaaagaa
//
LOCUS       DAFEOM010000020        35019 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.021, whole genome shotgun sequence.
ACCESSION   DAFEOM010000020
VERSION     DAFEOM010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.021"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(56..2653)
                     /gene="adhE"
                     /locus_tag="LP524_001176"
                     /gene_synonym="adhC"
     CDS             complement(56..2653)
                     /gene="adhE"
                     /locus_tag="LP524_001176"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBL2234241.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(2958..3338)
                     /gene="yajC"
                     /locus_tag="LP524_001177"
     CDS             complement(2958..3338)
                     /gene="yajC"
                     /locus_tag="LP524_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBL2234242.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(3435..4580)
                     /gene="tgt"
                     /locus_tag="LP524_001178"
     CDS             complement(3435..4580)
                     /gene="tgt"
                     /locus_tag="LP524_001178"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBL2234243.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            4798..5496
                     /locus_tag="LP524_001179"
     CDS             4798..5496
                     /locus_tag="LP524_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBL2234244.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(5664..8303)
                     /locus_tag="LP524_001180"
     CDS             complement(5664..8303)
                     /locus_tag="LP524_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBL2234245.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(8922..9953)
                     /gene="queA"
                     /locus_tag="LP524_001181"
     CDS             complement(8922..9953)
                     /gene="queA"
                     /locus_tag="LP524_001181"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBL2234246.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            10146..11321
                     /locus_tag="LP524_001182"
     CDS             10146..11321
                     /locus_tag="LP524_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL2234247.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            11325..11966
                     /locus_tag="LP524_001183"
     CDS             11325..11966
                     /locus_tag="LP524_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2234248.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            11963..12880
                     /locus_tag="LP524_001184"
     CDS             11963..12880
                     /locus_tag="LP524_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL2234249.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            12877..13548
                     /locus_tag="LP524_001185"
     CDS             12877..13548
                     /locus_tag="LP524_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2234250.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(14089..14577)
                     /locus_tag="LP524_001186"
     CDS             complement(14089..14577)
                     /locus_tag="LP524_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HBL2234251.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(14778..15044)
                     /locus_tag="LP524_001187"
     CDS             complement(14778..15044)
                     /locus_tag="LP524_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBL2234252.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(15044..15307)
                     /locus_tag="LP524_001188"
     CDS             complement(15044..15307)
                     /locus_tag="LP524_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBL2234253.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            15783..15968
                     /locus_tag="LP524_001189"
     CDS             15783..15968
                     /locus_tag="LP524_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL2234254.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(16110..17933)
                     /locus_tag="LP524_001190"
     CDS             complement(16110..17933)
                     /locus_tag="LP524_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HBL2234255.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(18531..21236)
                     /locus_tag="LP524_001191"
     CDS             complement(18531..21236)
                     /locus_tag="LP524_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBL2234256.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(21542..22489)
                     /locus_tag="LP524_001192"
     CDS             complement(21542..22489)
                     /locus_tag="LP524_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HBL2234257.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            22679..22948
                     /gene="rpsN"
                     /locus_tag="LP524_001193"
     CDS             22679..22948
                     /gene="rpsN"
                     /locus_tag="LP524_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBL2234258.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(23124..23486)
                     /locus_tag="LP524_001194"
     CDS             complement(23124..23486)
                     /locus_tag="LP524_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBL2234259.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(23497..24618)
                     /locus_tag="LP524_001195"
     CDS             complement(23497..24618)
                     /locus_tag="LP524_001195"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBL2234260.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(24634..24984)
                     /locus_tag="LP524_001196"
     CDS             complement(24634..24984)
                     /locus_tag="LP524_001196"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBL2234261.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(25114..26625)
                     /locus_tag="LP524_001197"
     CDS             complement(25114..26625)
                     /locus_tag="LP524_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBL2234262.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(26947..28299)
                     /locus_tag="LP524_001198"
     CDS             complement(26947..28299)
                     /locus_tag="LP524_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBL2234263.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(28452..29528)
                     /locus_tag="LP524_001199"
     CDS             complement(28452..29528)
                     /locus_tag="LP524_001199"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBL2234264.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            29724..31469
                     /locus_tag="LP524_001200"
     CDS             29724..31469
                     /locus_tag="LP524_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBL2234265.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(31749..32186)
                     /locus_tag="LP524_001201"
     CDS             complement(31749..32186)
                     /locus_tag="LP524_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBL2234266.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(32170..33543)
                     /locus_tag="LP524_001202"
     CDS             complement(32170..33543)
                     /locus_tag="LP524_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234267.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(33865..34353)
                     /locus_tag="LP524_001203"
     CDS             complement(33865..34353)
                     /locus_tag="LP524_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2234268.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(34375..34977)
                     /locus_tag="LP524_001204"
     CDS             complement(34375..34977)
                     /locus_tag="LP524_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBL2234269.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         9919 a         7476 c         6466 g        11158 t
ORIGIN      
        1 ttttccaagt tctttttcat ctattttctt atcttttttc tacgctttct tttgcttatc
       61 cgttgtaagc atcaatgatg atctgtttca attcagagat caatggttct ttcggattag
      121 ctgtcgtaca ttggtcttcg taagctaatt cagccatacg gtctacggtt gtatctagtg
      181 tttcttgtgt gactccttga gcttttagac tcatgttgat gccgacagat tttcctaatt
      241 cataaacagc cgttgctaaa gcttcgacta attcctctgt tgtttcgcct ttcagaccta
      301 agaattttgc gatatctgcg taatctgtat ctgcacggaa atagtcatat ttagggaaca
      361 tcgcatgttt ttgtggatct tttgcattat agcggataat atgcggtaaa agaatcgcat
      421 ttgtccgtcc atgagggatc ccgtattccc cgccgatttt atgtgcgact gagtgacaaa
      481 tacctaggaa tgcgttagca aacgccatac cagccattgt agaagcgttg tgcatttttt
      541 cacgtgattc catatcaggt gtttttactg atttttctaa atagtcaaag actaacttga
      601 ttgcttgtag gctcaatcca cgtgtataat ctgaagccat gactgaaaca taggattcga
      661 ttgcatgtgt taacacatcc atcccggtat ctgctgtgac agatgctgga actgacatga
      721 cgaattgagg atcgacgatt gcgacatctg gcgtcaatgc ataatctgct aacggatatt
      781 tcacatgtgt ttcactgtca gtgatgaccg caaatggcgt tacttcagaa ccagtccctg
      841 atgttgtcgg gatacatacg aattgtgttt tttctggttt gtcgatctta tatgtccgtt
      901 tacggatatc caagaatttt tgttttgcac caaagaaaga tgtgtctggg tgttcataga
      961 acatccacat tcccttagct gcgtccattg cagaaccgcc tccaagagcg attactgtat
     1021 ctggcttgaa gtctgccatc agtttcgttc ctgcgtatac ggtatttgtc gatgggttcg
     1081 gttcaacatc tgagaagact tcgatcttca catcattttt acgtcgtgcc aatacttcac
     1141 gaactgtatc gcagtaaccg aattggacca taccagggtc acaaacgatc atgacacgtt
     1201 caacgttttc cattttctct aagtacaata gagagttctt ttcaaagaag atttttggcg
     1261 gtaatttgaa ccattgcatg ttatttctcc gtttcgctac agttttgaca ttgattaagt
     1321 ttacagcaga gacgttacga gaaacagagt tcttaccata agaaccgcat cccaaagtca
     1381 atgacggaat catttcgtta tagatatcac cgatgccgcc ttctgcagat ggtgtattca
     1441 ctaaaatacg acaagctttc attcgaagac cgaatttgac atgcaactct tcgttttctg
     1501 aatggataac tgctgtatgt cctaatcctc caagattcaa cataccttca cacaaatcaa
     1561 acgcatggtc cgtgttgtta gctttgatca tggcaagaac tggagaaagt ttttctcttg
     1621 atagcggata atctgggcct acgccttcca attcagcaat cagcattttc gttccttttg
     1681 gtacactgat tcctgccaat tttgcgattt ccatcgcaga atgtccaacg attgatggat
     1741 tgaccgcata ttttccttcg ttcatcactg catcttctaa tttttgcaat tcatctggtt
     1801 ttacgatata aacttggtgt gcttggaatt ctgcttttac tgctgcatag atttctttat
     1861 ccacgatcac agcttgttca gaagcacaaa tcattccatt atcaaatgtt ttcgaaacga
     1921 tcaaatcgtt cactgcacgt ttaatttttg ctgttttttc aatataagct ggtacgttac
     1981 ctggacctac acctaaagct ggttttcctg tagagtaagc agacttgacc attccagctc
     2041 cacctgtcgc taagacgatc gcgatacctg gatggttcat tagcatagaa gtcgcttcga
     2101 tcgaaggatg ttcgatccat tggatacaat tttcaggagc tccggcagcg atagcagcat
     2161 cacgtaccac tcgtgctgct tctgcagaag atttttgggc acttggatgg aaagcaaaaa
     2221 cgattggatt tcttgttttc aacgcgatca ttgatttgaa aatcgttgtg gaagtcggat
     2281 tcgttgttgg agttactccg caaaccacac cgactggttc agcaatttca atcaaccctt
     2341 tttggacatc ttcattgatc acaccaactg ttttatcatg tttgatatta tgccaaatat
     2401 attcggacgc atacatattt ttgattgctt tatcttcgta aacaccgcgg cctgtttcct
     2461 ctacagccat cttcgctaat ggcatatgtt gatccaaagc tgccattgcc atttcatgca
     2521 cgatatggtc tactttttct tgatcaaagc tttccatttc tttcaatgct gcatttgctt
     2581 tttctgccaa atcatcaatc attgcttgta cgtcaattgt cttcgttttt tctttttcta
     2641 ttactttagc catcattatc ctcctaatgg gtcatggtta tttgttaaat aattcacatg
     2701 tatatcatac aatatttatt tttctatgta aacacttttt tgtgattttt ttcacagtta
     2761 tttttaaata taaaattgct ttatttacag gtgtttgtat agaaaaatat ataaagttat
     2821 ttattattat ttatatgaca cacccgatta ttcaatattg agcaatatat cccatgctct
     2881 tatctctttc ttttttgaat aaaaaatgag gtaggacatc tttctgccct acctcgtctt
     2941 taaataagct ttgatactta tttttcttcc ttctcttctg tctcttccag cgtttctgtt
     3001 gtttcagttt ttacttcaac tgtctcagct ggttcttctg ttgtttcaac agtagtcgtt
     3061 gcatcatttg ttacgacagt ccctggtttg accgttttga ttgctgcacg atcgtactca
     3121 aggaagatcc cttcgcagtc taaaacaact gtccgtttgt cattgtcgat ttctgaaaca
     3181 actccatgta atccgccgat cgttacgact gaatcacctg gtttcatgct atctaacaag
     3241 ttttgacgtt cttgctgttg tttcttttgc gaacgtgaca taaaaaacca cattgctaat
     3301 agcgcgataa acatcacaag cattggtaat gttcccattt ttttcacctc actaattctg
     3361 tatctacaaa aaacacttta gcagaaaaat gctgataaca ctagtttttt acttaatttt
     3421 taaaagattt tcctttagaa gctttttgcg ttttctttat tgaacccgta ttcttcaaaa
     3481 aaggcttctc taaattcaag caaattatca tccatgattg cttgacgtac ttgtttcatt
     3541 agattcaaca agaagtataa attgtggtaa gaagtcaaac gaatcccaaa tgtttcatca
     3601 catttgatca aatggcggat ataggcacgt gtatagtttt tacatgtata acaatcacat
     3661 ttctcatcaa ttggacggaa atcatgggca tatttcgcat ttttcactac tagacggcct
     3721 tgagaagtca tacatgtgcc atttcgagcg attcgagtag gcaagacaca gtcaaacatg
     3781 tctactccac ggataacacc atcgatcaat gaatcagcag caccgacacc catcaagtaa
     3841 cgtggtttgt tttcggggat caacggtgta gtgaattcta atacacgatt catttctgat
     3901 ttaggttccc cgacagataa cccgccgatt gagtaaccag ggaaatccat cccgactaga
     3961 tctttcgcgc tttgacgacg aagatcttca aaccctgctc cttggatgat tccaaataat
     4021 ccttgacggt ctggattagc atgagctttc aatcctcgtt ctgcccatcg agacgtacgt
     4081 tctactgatt ttttcacgta gtcgtaactt tcatcgaatg gtggacattc gtcaaaactc
     4141 atcatgatat cagagcccaa tttattttgg atgttgattg ctttttctgg tgataaaaac
     4201 atacgcgaac cgttcaagtg atttttgaaa tgcacgcctt cttcttcgat attccgcata
     4261 tcacttaatg aaaaaacttg aaagccgcct gaatctgtta aaatcggttg atcccagttc
     4321 ataaatttat gcagtccgcc agcttcttct actaagtctt caccagggcg tagccataaa
     4381 tgatatgtat tacttaaaat aacgccagca cccatttctt tcaactcttc tggggacatt
     4441 gtttttactg tagctaatgt tcctactggc atgaacattg gtgtggggaa agttccgtgt
     4501 ggcgtgatca attcccctaa acgcgcgcca gtatgttttt ctttcttgat taaacgatag
     4561 cgaatggcag gttgcgtcat tcatttccct actttctata taaaatatct tgacaactcc
     4621 caccatcata atcgtttcaa gtgctttttg caagttccct cggcttcctt ctactttatt
     4681 taatattctt ttttttatcc tttttttatg aataacaaaa aaaagaaaaa cattatgaca
     4741 gaaatatttt ttgatacatg gtatattggt gttattaaag aaaaggaggg aaaaatcatg
     4801 caatcttctg cagaattaaa acgccaagca aaagagagtc taaaaggacg ttggggacaa
     4861 gctgttttac taaatctaat ccctacattg attggtattg ctactattct tcttattatg
     4921 attcctatta tcttgtttgc caccaccata tatgatggat cagcgagcgt ctcagaagcg
     4981 acaacttcag gaggcggtag ttcaggaggc ggtggcggaa ttatttccag catcattagc
     5041 gctttgttca tgagcggtat ttcttggact tatcttgata ttttacgtgg acaaaaagat
     5101 cagatcgaac cattcaaaga tgcatttcgt ggattcaaag gtttgtttat cggcggagtg
     5161 atcagcttag ctgtgctgat ctcgatcttt acaacattat ggggtttgct attagttatt
     5221 cctggaatca ttaaaagcta ttcttattct caaagctatt ttatttacta cgatatcgtc
     5281 caagaaacag gacaaacacc taaagtactg gatacgatca ctgcaagtcg aaaattgatg
     5341 gatggttata aaggacaact tttctggtta gatctttcat ttatcggctg gcatatctta
     5401 gcattagcaa ctgctggtat tggttatcta tggctcagcc catacatttc tgcaacaaaa
     5461 gcagcatttt atgaacaatt gccaaaagat atttaaaaga gagtgtgact tgtgaccgag
     5521 ggggtggcag gagaacaccg tttatcgaga ggcagtaaaa gaaacattca gcccattagc
     5581 aaaaaggagg tgtgacaaca ttcatgtcac acctcctttt tgtatagttc cagtaaaaag
     5641 aaaatcaact tagaaacagt taatcattcc tcttcttctg cttccggtaa tcgatggaac
     5701 ttgatcagtt cattcaccgc gattggtacg atagataaaa tccctaccaa gaaccagtga
     5761 ttcaaactga tgtgtactag gccgaatagt tcttgcgtaa atggcagcaa agcaacagcc
     5821 gttgtaatag ctaatgccag taacgccatt tctaccaaag ctttattggt cgctaaattg
     5881 actttgaaga tcgattggct gctgcgtaca ttgaacacat gcagaatcga tgaataagct
     5941 aagatcaaga atgcaacagt ttgaccgaca tgctgacttg cgtctaccca tgggctgata
     6001 gtatcgacat aactacctaa ataataacca aacaaggtta cgatggtgaa gacccctgca
     6061 tttatggcaa ttttttgcaa taaacctcga gcaaagatcc cttcattttt cggaatgggt
     6121 ggctgatcca tgatgccgtg ttccgctggc tctcttccta aagcaaatcc tggaagtcca
     6181 tcagcaacta cattgacaaa cagtagttgc acagctgtaa atggtgctcc ccatcctaac
     6241 agcatcgcaa tcaaaacaat aaagatctcc gaaatattac agcttagcaa aaagttgata
     6301 gctttacgaa tattctgata aaccgaacgt ccttgactga cagcgtctac gatcgtcgca
     6361 aagttgtcat ccgtcaaaat catatctgcg gcactttgcg ccacatctgt tcctgtgatc
     6421 cccatggcac acccaacgtt acttgctttc aacgctggtg catcattcac accatcccca
     6481 gtcatcgcta cgactgcacc cgtacgttgc caagatttga cgatccggat cttgtcttct
     6541 ggcgttaccc gagcatagac agagagagct ttgacacgcg catctaattc ttcgtctgat
     6601 aattgatgca gttctgatcc tgaaagtgct tctgacggat cattcaagat acctaattct
     6661 ttcgctatgg cactggcagt caccacatga tctccagtga tcatgactgt tttgatgcca
     6721 gcctttttcg ctcgcttgat tgctccttta ctttctggac gtggtggatc gatcattccg
     6781 atcagaccaa tcaaacgtaa gtctttttcc agtgcttcag aagaaatatc ttgcggttct
     6841 tcgtcataag tagcataacc aacagcaatc actctcagtg cccgtttacc aaagcgatca
     6901 ttaacgatcg ccgcttgttc tacatcgcct gaactaaagc gcggagcaag aacatcgaat
     6961 gctcctttgg tgattgagat gtatttttcg cccatttggt gaactgttgt catcattttt
     7021 cgttcagaat caaaaggaag ctcgtttact cgtggatatt tttcttctaa ttctgccttt
     7081 gtatgataat tctcttcaac agcccttacg atggctactt ctgttggatt acctttgata
     7141 acaaggtcgt catcttcttg ctcgaccgtc acatctgtac atagtgcagc cattttcaat
     7201 atttccatcg cgctgttcgt cattgaatct tcgatattcg ttacttcatc ttctttgctc
     7261 catacccggc ggacgcgcat ttcattttgt gtcagcgttc ctgttttgtc tgagcagata
     7321 acattagcgg ttcctagcgt ttcaactgct ggcaactgtc ggatgatcgc atgtttcctc
     7381 gccatcttct gcacaccgta tgcaagtgtc aacgtaacaa taacagtcaa ggtttccggt
     7441 acagctgcaa cagccaagga aatcgaagtc atgaacatct caaggagtgg ttctccttgc
     7501 agttcaccaa tcacaaatac taaagccgca gccgctaaag cgatcatact gattcgtttg
     7561 cctagctgat tcaatcggac ctgtaaaggt gttttttgca tagtattttc gttcaaaaga
     7621 tccgcgatct tacccatttc tgtagccatg ccaatggcag tgacgatcgc tttcccacga
     7681 cctgcagcaa ccgtacatcc tttaaatacc atattaagtt ggtcacctaa tgactcatct
     7741 tcttcagcaa taaatgctgc atctttttca actgcttcac tttctccagt caacgccgat
     7801 tcttctacac gtaattgtga tgcttcgatc aaacgggcat cggcagaaat agcatcaccg
     7861 gactctaaaa caagaatatc acccactacc agttgctctg actcaacagt ggtcactttg
     7921 ccgtctcgta tgacagttgc cgtttgttta ttcatatctt gcaaggattc cagtgctttt
     7981 tctgcgtttc cttcttggac aatcgccaat accgagttga tcacaacgat agcgatgatc
     8041 agcagtcctt caaataaatc cccatgttct gtagcaaatg cggtataaaa ggaaatcacc
     8101 gctgctacaa gaaggatgat cgttgtaaaa tcggacaagc ttcgaaagaa ttttttgatc
     8161 atcgattctt tcggcgcctc atcgaattta ttcaatccct gcttttttcg ccgttcatcc
     8221 acttcttgtg aagacaaccc agtatctgga tctgcttctg ttaccttctt tacttcttct
     8281 attgtctgct tgtattcttc catcctattc ctcctttaaa atcttcgtac aaaaaaaggt
     8341 acttatttta ctataacatc ctttttttat ttacttcaaa taaagggctt atcgattatt
     8401 tgttaagaaa gcgtaaaatt taaagtgctt atatttgcaa acgctaccaa aggatgctat
     8461 tatatagatg taaggaagga ggataccatc aaatagacag aagaatttct cgatagaaca
     8521 gtgtaagcaa acaaagttgt tagaagtaca ggtttcaccc agacatgcgt gtagagtaca
     8581 aacctataaa tagtgaatgc gtatggatgt ttggatcacg aagtatccga aacaagaagt
     8641 aatgcgatac gtaacaattg aataacgtaa acgtctgaaa attgaatagg aacaagagat
     8701 gtttcaaatg ttgaagatgt aacgaaaaca aaatttttgt ttaacatgaa gaaattcaac
     8761 accgtgcttt gcatgtatga tggaaagtta caatcatttc tgtttatttg aatccttccg
     8821 aggtacaaga aagccgagct tatcgagagc tcggcttttt ctttgtcata aaatctttgt
     8881 aaagcttttt tgttattcaa aactagcggc ttttcataac cttatttcac gaacatcgca
     8941 tctccaaaac taaagaaacg atagcgctct tcaatcgcat gctggtaagc agataaagtc
     9001 aattctcttc ctgcaaaggc actaactaac atgactaaag tcgattttgg caaatggaaa
     9061 ttagttgaga aagcttctac cactttgaat tcatagccag gcgtaatgaa aatatcagtc
     9121 catccgctgt ctgctttgat ttctccatta aatttcgttc caatcgtttc taatgttcga
     9181 attgaagttg ttccgacagc cacgattttc ccgccatttt tacgtacttg gttcaatcgc
     9241 tcagccgctt cttccgtcaa acggtagaat tcactatgca tatggtgttc ttcaatttta
     9301 tctacgctga caggtcggaa tgtacctaac cctacatgta gtgtcagata gactaattca
     9361 acaccctttg cttcgatctc atcaagtaat tctttcgtaa aatgcaatcc agctgttggc
     9421 gctgcagcag aaccattttc tttcgcatag acagtttgat agcgatctgg gtcttccaga
     9481 cgttctttga tatatggagg caatggcatt tctcccaatg attcaagaat ctctagaaaa
     9541 attccttcat actcaaagtc aatgatccgt ccgccgtgtt ccaattcttc tttcactact
     9601 gcttgcaatc tgccatcacc aaaagagatt cttgtcccaa ctttggcacg tttcgcaggt
     9661 ttgatcaatg tctcccacgt atctccttgt gtattcgtta gaagaaggac ttctaaatgc
     9721 gcaccagtat ctggtttttc tccatataaa cgagcaggca gaactcgtgt attattcata
     9781 actagcgcat caccaggctg taactcttca atgatttcat ggaaatgttt atcttccatc
     9841 tctccagttt tccgatccaa aacaagtaaa cgagaatgat cgcggtcttt taatggtgtt
     9901 tgtgcgatta attcttccgg caagtcaaaa tcaaaatctt ctgttgttaa catatcgtca
     9961 ctcactttct atcatgtgct tcatcattct atcatgtttt tcgttctgtt ggcaaaatca
    10021 gcactttttc acttctattt tttgttttcc taaataaaaa aactacccta tttgtatttt
    10081 ttcattgttt taggcatact agtaatagat agttcttcat tgaaaataga aaaagaggtg
    10141 gcaccatgat tgaatttcaa aatgtgtcaa aaatttataa aggtggaaaa gtcgctgtag
    10201 aggacgtcaa tctttcattt gaaaagggtg agttcatttg ttttatcggt acgagcggaa
    10261 gcggaaaaac gacgacgatg cggatgatca accgaatgac tgatccaaca caagggaaaa
    10321 ttttgattga tggaaaatcc atccaagaaa ttgatccagt ggaattgcga cgtcagatcg
    10381 gctatgttat ccaaagtatt ggattgatgc ctcacatgac gatccgtgaa aatatcactc
    10441 ttgtccaaaa attgttgaaa gtcagtcaag aagaacggaa taaaacagct gaaaaaatga
    10501 ttgatcttgt ggaattgccc cgtgagatgt tagatcgcta cccacatgaa ttatctggtg
    10561 gtcagcagca gcgtattgga gtcgttcgtg cccttgcagc gaatcaagat atcatcttga
    10621 tggacgagcc attcggtgcg cttgatccaa tcactcgtga ttccttgcaa gaccttgtca
    10681 aagatttaca agagcgtttg ggaaaaacga tcgtatttgt aacccatgac atggatgaag
    10741 cgattaaact agcaagccgt atcgccatca tgagtgaagg ccgtgtgatc caatttgata
    10801 caccagaaaa tattttacgt catcctgcaa acgatttcgt agaagagttg attggagaag
    10861 atcgcctact acaagccaag ccaaatgcga caagagtcgg cgaagttatg ctaaatacag
    10921 caatcacgat tacaccggaa aaatcattac aagaagcaat caaactgatg agggaaaaac
    10981 gtgtagatac gttgttagtt accgataatt cgaatgtatt aaaaggattt atcgatgtag
    11041 aaacgttgaa taaaaaacga ggaaaagtat ccagtgtcgg cgacattttg aataaacatg
    11101 ttttttacgt aaaagaatcc gcctatcttc gtgatacctt gcaacgcatt ttaaaacgcg
    11161 ggctgaaata cgtgccggtc gttgatgaac agaacaaagt cgtaggtatc ctgactcgtg
    11221 catcattagt ggatatcgtc tacgatgtga tttggggaga agatcccgca actactgatg
    11281 ttgcagagtc agctactcct gaagaatcta aggaggtctg acctatgcag cagttttttc
    11341 atcagcacgg ttcagaaatt ctctcaaaag gttttgaaca tctttatatt tcaggacttg
    11401 ctttgatcat tggtatcatc attgctgtac cgcttggtgt cttactgaca cgttttccaa
    11461 aagctgccac gattgtgatt ggcttaacca gtgccttgca gacaatccct tctcttgctt
    11521 tattggcatt gatgatcccg ctatttggcg taggtaaatt acctgcgatt atcgcactct
    11581 ttatttattc attattgccg attttacgta atacgtacat tgggatgaaa aatgtcgatt
    11641 ggaattatcg ggatgtggca aaaggcatgg ggatgactga gttccaatcc attttttccg
    11701 ttgaattgcc cattgctatg ccaacgatca tggctggtat tcgtttagct gccgtttacg
    11761 tgattgcatg ggctacactt gcctcttata tcggcgctgg tggtttaggc gatttgattt
    11821 ttagtggact gaataattac caaccagatc tcattttggc aggtacgatc cctgtaacaa
    11881 ttttagcact attggctgat tggcttctag gtctcttgga acaccggctg acaccgatcg
    11941 cacttagaga ggagaatgaa gaatgaaacg tatactaaaa atgaccatgg ttcttttctc
    12001 tcttcttata cttgcgagtt gttccttccc tggtcttgcc agcaatacag atgaagacac
    12061 catttcgatc acaggtggaa tcacttcaga agcacagatc ttagcaagtc tagttgccgg
    12121 aatggttgaa cattatacag ataaaaatac cgcaatcatc aacaatctgg ctactacaac
    12181 tatcaaccat caagcgatga tgaacggaga tgcttcaatt tcagcggctc gctacacagg
    12241 aacagattta acgactacat tgaaccttcc tcctgaaaaa gatccgaaaa aagcgtttgc
    12301 taccgtgaaa gatgaatttg agaaaagata cggtcaaacg tggttcccgt cctatggttt
    12361 tgaaaatacg tatgtctttc ttgttcgaaa agacaccgca caaaaatacc atctatctaa
    12421 agtcagcgat ctgaaaaatg tagcggatga attagtagct ggtgtagata cttcttggat
    12481 caatcgtaaa ggagacggtt atgacggctt ccaagaaact tatggcttct ccttcaattc
    12541 gatattacct atgcagattg gacttgttta tgacgcagtc gaagcaggaa aaatggatat
    12601 tgttcttggc tactctacgg atggacggat cgccagttat gatctagtca tgttggaaga
    12661 tgatcgtcaa ttcttcccac catatgatgc tgctccagtt gtagacaaca agatattagc
    12721 tgatacgcct catttaaaag aagccttaaa taagttaggt cataccatca gtacggaaaa
    12781 aatgcaggaa ttgaactacg aagcagataa taatctagtg gaaccatcag tagtagcaca
    12841 acgtttctta caagaaaacc actattttga agggaagtga agcaattgga taaaatgaat
    12901 gtctttcaac aatttttcta ttatttccag gaaaatggct cctatatctt tgcacaattt
    12961 atccgtcact tcctgatttc tatctatggt gtcctctttg cagcagttgt cgggattccg
    13021 gttgggatca tgatttctag gagacgtaaa ttagcaaact gggtgattcg tttggcgaat
    13081 atcatccaga cgatcccttc tctagccatg atctccattt taatcattgg attaggcctg
    13141 ggagtcaatg tcgtgattgt tactgtattt ttgtattccc tcttaccgat catcaagaat
    13201 acgtataccg ggatgatcca agtagacaaa aacatccttg atgtcggcaa aggcatgggg
    13261 atgactgcta gacaacgact attcatggta gagttaccac tctctgtttc tgttatcatg
    13321 gcaggcataa gaaacgcgtt agttgttgcc atcgggataa ccgcgatcgg tgcattcgtc
    13381 ggtgctggtg gtcttggaga tatcattatc cgaggaacca atgcaacaga gggcacatcg
    13441 attattttag caggtgcatt gccaactgca ttaatggcga tcatcacgga ctggctttta
    13501 ggtattttgg aaagacggct agatccagcc agccgtacat cacgctgatg ttattgtaaa
    13561 aaacaaaagc ttctgcgacc atttgttggt cgcaaaggct tttttagttc tgtcattttt
    13621 aaggcaataa tccactctga ttcttccctc cttatgggca aagagttgct tttcttagct
    13681 atttatccat tccgtatact ccaacttcat actgccaacg ggttcatatc tgcatccacc
    13741 gactaagttt ttttcttatc cgctaaggca ttttgccaat ccattttccc atcactcgtc
    13801 atggaattca taccacatga tctctaaata ctcgtctgct tgttctttga attgggcatc
    13861 aattcttaca tcctttggcg tggctttcat ttaagccttt tccatcagtc ctcgattgtt
    13921 cgatgccttc agctgcatca tcaaaactgg tgactgccgt atcggtaact tcgatcatct
    13981 tttatacaat cgggaacttg ccatcctcat ccttggctaa aaacatatct agccattcca
    14041 gcttctcgcc atgggaggtt tgcggttcct aaaaccggta cacgattctt atggactgca
    14101 tccgtcgcaa ctagacttgg cgatacgatg atccttcacc agaagagcac ccccgtaaac
    14161 caattgatcg atatattgcc aataagaaaa gacattggcg taaaagatat cgatccctcg
    14221 tgatgcattg ccgttgatac tgctattcat ggccgaaagc gccacgactt taacttcttc
    14281 cgtttgagtg acattcatgt tcggcaatgt cgctttggat acccgctctg ctaaaggaac
    14341 gacactggta ttgtatttcg tatctgtatc ttttccaaaa gaccatgcca acgaatcctc
    14401 tgggaaccaa taagacgact ccggttgatt gtccattgct tttttcaaca tacttttggc
    14461 tgtttctcga taaacgacat ttccaattgt cttcgcctca ctttggcgat aatcgatggt
    14521 cgttgccact gccaacacta acaacgatgc tccaatttta ctgattttcc ttgtcacact
    14581 tcttcctcct tgttttacat aaacccgtca acattccatt cctaaactca aacaatgacc
    14641 cttcaaaggt ggagatatag gaacaaatta aagaaatcat gcaataaatc caagaaggtt
    14701 ggttttagaa caaaaaaaga gtctcctttt tggggagact ctttgttaac tatctaaaaa
    14761 ggttggctct tctcacttca ctcataatgt gtccaggcag aataaatttc tacaacttta
    14821 ttttcctcat caactttgta aaccactcga tgctgtctat tcagcctgcg tgagtatctg
    14881 ccttcatgtg taggtctcag cttttcaaaa gactgcgtgg gcatatatgg atcttccttc
    14941 aatgtctgaa tcacttcttc aaattgtttc tttaaattcg tttgcttgat ttttcttaaa
    15001 tccactttcg cagagttttt gattgcgact gtatagttac tcatcaaaga gcttcccaat
    15061 caatatcatc gatattcgta aagctactgt cgtctttttc gcggtctcgt actttatcta
    15121 aagtacctgt ttgctcaaga aaaagggttt cttgaatcgc tctccagtct tccagcccaa
    15181 tcaatacagc attgtttcca ctacgatcac taataatctc gatttcagta tgattttcat
    15241 ttacctcttt caataactga taaaaatttt tacgagcagt gctaggattc atcgttgtat
    15301 tagccattgt ctctcctcct ttttctgata taattatagc gtacttgatt aagtacgtca
    15361 aaaaaataca ataaagcact taaattcagc aaacttttcc tcttttttac atagagaaaa
    15421 agaccccttt ttagggagga tctttgtcca cgctcacttc ttactccttt acgactagcg
    15481 gtagccaaac gtaaagtaat tggtaacaac gtatcatctt tagcaaaggt cgaatccaaa
    15541 gcctgtgagt ctatgcaaca acaccaatac acgaaggttc ctcgttgcag tgtgacttga
    15601 ttgatatctt gaataacctg aggatacgca cgaaagattt gtaacggtta ttcaaaactg
    15661 attttggccg tcaagtcttc tactgcctca aaaatgattg cctctttcgt ctcctgtcat
    15721 tcatgttcac tatcgatttg atacttgctt agaccgttgg gtttgtgaat caccaattaa
    15781 aaatggcact gagttatggt aacaaaaaat ggtcacttga cgaggctgag attcctactt
    15841 atgaagacac atggcactta agagtcgagg taaaggaaga agctgaaaat ataggaggct
    15901 gtacattatt tggcaaagga ttcggaaatt atgatcaaga catcaaaatc aaaatgtttt
    15961 acagctaaat aggaagaata ataacatttc atacgctgtt cccatcttta atggcttata
    16021 gagggcacct ttagctgaga ataagacaaa acagaccctc tagcttcaat taagagctag
    16081 agggtctgtt tttgatttta aaacttggtt tatttcttca aatggtagct ataggtgctg
    16141 acaacgatat tttcacgcca agacaatcgc aatcttaacc gcaaacttgt ttggttatgg
    16201 agaatattct gccagcgtct gtttggtaca aattgaggaa tcaacacagt agttgtatag
    16261 ttttgtttcg ctgcattttt acttacgata tctacataac ggataattgg attttcaatc
    16321 gagcgataag aagagtggac gattgaaaaa cggacatctg ggaaatgctt tttaaattct
    16381 gcttcgattt ctttttcttt ttcgacatct tcgtctagcg aaacatgcat cgcaacgaca
    16441 taatcaccga tcgaacgtgc gtaattcaac gcaccgatat ttacccgtgt cacattcccg
    16501 actaatacaa gaacggtatt cccgtcataa tcatgtagtt ttgcatcatt ttctaaacga
    16561 agctgttctg ctactttttt ataatgatca tggatcttat aaaaaatgaa cataacgatt
    16621 ggcataatga tgaagaaagg ccaaatgtcc cctaaccgat agacaaacaa aatcgcaatg
    16681 attgcatatg agatgaacgc acccgtaata ttggcaatcg atttgcttaa ccatcttttt
    16741 ccttcttttt tccatttgac gaccatccct gtttgagata aagcaaatgg aatgaacacg
    16801 ccgatagagt ataacggaat caagcgttct gttgagccat ggaaaataaa cagcaatacg
    16861 atcgaaccta gcgctagtgt gataatccca ttggaatagc ctagtcgatc cccacgatct
    16921 tgatacatgt gaggcatgaa tttatctttt gccagattgt atgcaagaac tggaaaagca
    16981 gaaaaaccgg tgttagcagc aactgctaaa atcagcgctg tagcaaattg aaggacgtaa
    17041 tacaaaatac catgtccaaa gacagcttta ccaatttgag acaaaacagt gacttctttt
    17101 tcaggaacga tcccatacca atagttgata aatgtgatcc ccacgaagaa gaaacctaag
    17161 atcaatgcca tcatcgtcaa tgtagcagcg gcattttttg ctcttggctt cttaaagaat
    17221 ggtacagcat tactaatcgc ctccacccca gttaaagagg aagaaccaga agaaaatgct
    17281 cgtaaaatca aagcgataga aacacccggc acagccgctc ctggaagtgc agtagcttgc
    17341 aatggcacag cacctgtcac gatattatat aaaccaacaa cgatcagtaa cgagatcaca
    17401 aggataaatg tgtacaccgg aaataatagg aacgatgcag attccctcag cccacgaaga
    17461 ttcagcatca tcagtagcaa aacgattgtc acagaaatag cgacttgatg accgtagagt
    17521 gctggcaccg ctgatgtgat cgcttcagca cctgctgaga cagatactgc tactgtcaac
    17581 atgtagtcga tcaataaaga gccaccggaa atcaagccag cattctttcc aagattctca
    17641 ctgctgacga catatgctcc cccgccatga ggataagcat ggatgatttg acgatatgat
    17701 agcgttaatg aaatcaataa aataataaca aaagcggcaa tgggtagcga ataccagatg
    17761 gctgcagcag acaaagcgac taaaacgaca acgatttgtt ccgtaccata tgcaatagat
    17821 gataacgcat cagacgacaa taatgccaaa gcagcaaatc gggttaattt gtgctcatca
    17881 ttttcagcag attttaaagg cttacctact aacaaccttt ttaagtaatc caatttctaa
    17941 actcctttac acacaatttt acttcaagtt ttatagtcga aaatttatag taaacaagat
    18001 gttatagtac gctcactgac cttaaaagtc aataaacttt aagaaaataa ttggaaaaaa
    18061 acagaaaaat atcaagcgct ttttcaaacg ctttaataca gcaaaaatct aacatttttt
    18121 tatgggcttt ttctttgaat tctataataa aaggatagaa cttgacttag aaaatcaagt
    18181 tctatccttt ttgatatctt aacttttgga agagagatgt aaggctgctt tctacccatc
    18241 attcttacta ctcgaagcat agcgcagttg tcacaacctc ttgataaacg gtgtttaaaa
    18301 gcttgcatct gcggtaataa gctcaaccaa attaaaaaca taagaagctg tttttatttg
    18361 gtcgttctta ttacttgatg cagagcagct tttttacaac ctcttggtac acggtgttca
    18421 atcagctgac cctcggtatt agctcaaaaa aacggcaaaa tatgaggaac tatttttcca
    18481 tttttttctc taatactcag gtcaaaacac tgatttcaca acctcttttt ttaatgaaaa
    18541 atatggctct catgcgccgc ttctttgaat ttcgtcattt tttcttcgat gaacggatag
    18601 attgccgttc ctacgattcc aaagacaaca aacaaaccga tcatgatcca gattgctttc
    18661 gtagcatttg gccaataaat tcctcctgca gattcccgta acaagtttac cgcatgagta
    18721 aatggcaaga acggattgat catttggaag aatttccctg agacttggat cggatagttc
    18781 ccgcctccgc cagagatcga cagtactaag atgataatag cgatcccttt accgacatta
    18841 tcgaataatg cagccaagac atagacgatc atcatgaacg ctaaggcgat caagacagca
    18901 aatgacacgc tgtagaccgg attttggaca tctactccta gtaagaaaat attccctaat
    18961 gtaacgatca agctttgcgc aatcgccatt gtcaaaaatg tcagcatacg agcaacgaat
    19021 tgttctcgtt tcgtaaaatt gtttcggtct ttctttccta aatagtattc tgtcgtagca
    19081 acacttgaaa gaagaacagc gccgacccac agacatagtg cagtataaaa cggggtgcta
    19141 gctgaaccgt tatttgcgat tggataaagc gcattggttt gtagctcaac aggttgtgta
    19201 aagaagtcac tttcttttgt tgcgtctagt ttcaataatt tgatgacttc gcctagatcg
    19261 acttcttgtt cgccttttcg gatcgcgtct gctgcttttt ggataccagc tttgatattt
    19321 ggccagtcat tgttgatcaa gtccgcagca gcgttcacag ctgtttcgac ttctggcatt
    19381 ttttcattta ctgttgtcaa tgtaccggtg atttcatttt ttagtgctgg ccaatcattt
    19441 tggataaaat cagcggccaa tcctaatttt tgctgaatga tcggtaattc attgttgtat
    19501 aggtctgccc cttcattgat gccattgacg atggtatcca tatttccatt cagcattgta
    19561 ttggcatcat ggatttcttg tccaattgca ggcatttctt tttgatattt ttctaaaatt
    19621 gccactgcat tcgttacagt tgctttcgtt gagctgagaa gcgaatcaaa gtcgatctgg
    19681 tccgctttgc tcagtgcgcc ttgtgcagtc ccaatcgtat cgatgacttg gtctagtaga
    19741 gaagtgacag tggaactgat ttgacctgta ttgataccag caatgatgcc attgatatta
    19801 ttaacaacgg catcaatttg atctagcaat tcatcaatag ccttcgtgtc ttcattttga
    19861 acagctgtgt tcaaagcaga taggcggttt tgcagattgc ttagcaaggg ttttaattga
    19921 tttagctgat caataattgg ctgtaatttc gtatttccag cacttgtttg caatttagtc
    19981 aggaactcga ccagtttatc gattgcattt tgttgttgag atattccgtc aataaagccg
    20041 ttcaatacat tgctgatgtt tttcttttca tcatcagtca gcttaccatc ttcaatagct
    20101 gttcttacag tatctattaa cgaacctgtc gttgttgcca cttgtccaat tgacttcaat
    20161 gtaacatcta cactgtttgt gatgcttggt atcgcatctt tcagctgaga ggcacctgat
    20221 tttgtttggt ttgctagatc acttgcctga ttttctaaat ttttcacatc tggcaagata
    20281 tcttgtactt gttggatgat cgtcaagcct tgttttgctt catcgattcc ttggttcatt
    20341 gtctcttgga tcgaagcaaa atcgccgtcg atctctgcta gttgcttacc ggcattttgg
    20401 atctctggga ttttttcttg caaatcaagg atgatttttg cttgatcttt caactcaggc
    20461 attttgtcat tcaatgcaac gactttttca cccatctcat ctactttagg gatgtaatcc
    20521 acgaattcat ttgctttatt cagtttttcc ttgatttcag gtagttgact ttgtaattcg
    20581 aggacttgtt ttgtgtagcc gtcgatcgtg tccaaatttt catctgttga taaaatcata
    20641 tctttaactt tattgatact tactaggttc gtatcgatgt cgtaacctat ctcattgaat
    20701 actttcaata aagtcgaact ggctgttttg ataaattcat ttgtgatttg cgattggatc
    20761 gaacttgctc ctttatcggt gattttcggt gcaatcgcat tgattttttc atttaccgta
    20821 tattcgattt ttggtttctt gatatcacca ctagtaaaac tcagcagatc ttctgagaaa
    20881 tcctttggca agtaaatacc tgcgtaatac tttccggaac ggactccgtc ttctaattgt
    20941 tcttttgaat caacgaattg ccatcctaat tgtttatttt tatgaagcga ttcgatgact
    21001 tgctccccaa tggccacttc tttaccttga aactctgctg gcttgtctgc actgtaaaca
    21061 gcaattggca gctctccagt atttccatat ggatcccata atgctttgat gttaaaccac
    21121 gcatatagcg aagggatgat catgatcgcg atgatcagaa gtgttgcaat cgggtttttg
    21181 aaaatccgtt tccagtcaag caaatataat cgaagcgtat ttttgattga ctgcatagtt
    21241 ctcccccttc ttcttttttc agctgaatta ctttaccatg tatttcatac ttttttcaat
    21301 cggctttttt caaaaaagtc gaatttttgg acatatcacg gcaattgtct cattgtaatc
    21361 tcttacatat tgagaaataa ttaaggatta cagcctttac tttatcatga aataaaaggg
    21421 aaagataatt ttatgccaaa gtttttatct attatacgta tacgcaaaaa agctaaaaca
    21481 ttttttgtct ttgcccctac aaaaatgttt cagctttttt cgttttttat tcctgtattt
    21541 cttatttttc gaatgacagg atgattttcc cagcaggagg atgtccttca actagctgat
    21601 gtgcttggat gacatgctta agtgaagcag acagattcat tgaaataaaa acatgaaagg
    21661 ctcctttttg gtaggctttg attatgccag caaaggcttc tgcatctaga tattcctttc
    21721 ttggaacaaa cgattcataa aagccttcct ttttttgccg caaatctaga tctgggaggt
    21781 catttaatgc aacatacctg ccgcctggtt tcatgatctg gatgccggtt tctcctttga
    21841 tacttccttt cgtcgcatcg attacaaggt ctgcctgatc agcaaattgc agacctggat
    21901 ttgttttatc ataagcagca aatgctgaag ctcctaattt ttttaccttt tcctcatttt
    21961 tactggatgc actggttaag attcggattc ccttttcatg gagaagctgg atcagacttg
    22021 acccgaccgc acctgatgct cctagtatca ttacagtatc tgtcggctgg atttccgtca
    22081 aatggttgat tagattataa gccgtaattc ctggcgtgac cattccggct gcttcttccc
    22141 aactcatttt atctggaatt tttgctactt ttgctgatgg caacacgact tcttctccgt
    22201 aagtcccgcc gactgcatgg acagctactc gatcacctac atgaacagtt gtgacatctg
    22261 aaccaatctc tgtcacaata ccagcgccat cattacctgg tacataaggg aatttcaatg
    22321 ttcgtatctc tttcatttct cctaaacgca aggcaacatc ataaggattc acactgaatg
    22381 cttttatttc tacacgaaca tgtccagaac cgacttctct gggatgagca tcgatttctt
    22441 caaaaacatc ttgtgcttct ccgtaaccgt taattgcaaa tcttcgcatg gtcaccatct
    22501 ccctttttct ttaagtttac gtttatgtct ttattttgac aaaaaaaatg tcttttcgtt
    22561 ttggcgcatg attcaaaaca aaatagttgt cttttttcgc gtaacgtgtt aaattattta
    22621 tctaaaagcg taaagattac gatttacgct aataaaaaat aattggagga atttgttgat
    22681 ggctaaaaaa tcaaagattg ccaaagcaaa aaaacagcaa gaaacgattg aacgctatgc
    22741 tgctttacgc caggaattaa aagcgcaaaa agattacgaa ggcttgcgta aacttccttt
    22801 agatgctcgt ccagaacgat tgaaaaatcg tgatttaatc gatggacgcc ctcgcggata
    22861 catgcgtaaa ttcgggatgt cccgtataaa attccgtgaa ttagcacata aaggtttgat
    22921 tccaggagta aaaaaagcca gctggtaatc caagtctggc tgtaatgtaa aggaggaggg
    22981 cgcatggacc actccttgga tcatcaaaaa agatacgttc gcttcttcta gcagaaatgc
    23041 tggtactaga agcagacgta tcttttttta cgcacagctg gtcctttttt atatgtaaag
    23101 gctgcatggt tacttcgcta tatttattta gtaacaggag tcagtgcttc gatacccaca
    23161 ctaattttca atgctcggtc aacttctgcc ataatgggtc gatccaaatg gcagaccttt
    23221 tctttcaatc ttgatttatc gatcgttctg atctgctcca acaggataac ggagtctctt
    23281 tctattcctg ttccagtagc tttgatccca atgtgagtcg gaagcttagg tttagccatt
    23341 tttgccgtta tggccgcaac aatgatcgtc gggctaaaat ggttacctag attattttga
    23401 ataaccaaaa ctgggcgtgt tccgccttgc tccgatccca cgactggaga aagatcagca
    23461 aaataaatat ctcctctttt taccatagga tcctccttat tcttgatatt ctcttggtat
    23521 ccgctgtcct aatccgcaag ccacttcata gtggatcgta cctaattgat ctgctatatc
    23581 ctgcatagtg atttctttgt ctgcattttt gccgataagc gtcacttttg ttccgacagg
    23641 aaattcttga ggcaatcgga tcatcagttg atccatacat acacgcccaa tcgtttcaca
    23701 gtagttccct tctaccaata gagaaaaacc ctgcatcttt cgtggccagc catctgcata
    23761 accgatcggt atcgttccga tccactctgc ttcaggagtg atatatgttt ctccatagcc
    23821 gattccttca cctgctggga gttttttgac ttgaatcaat tcagaaacca gctcaagtgc
    23881 tggctgcaat ggataaactt ccggtagtgc gtgcccagag ggattaagtc cgtacattgc
    23941 tactccataa cgaatcatat tgccgattgt ttcatcatgc cataaagccg ttgcactatt
    24001 gctgacatgt acatatcgag gaagttcggg aagttttttc agcacttcta taaagcgttc
    24061 tttttgcaga ttccaatatg tatcatctgc ctgatctgct gtagaaaaat gggtaaataa
    24121 accttcccat tctaatacac gactttcttt gatgaatgca gctgcctctt ttacttcttc
    24181 aggtgtacaa aatccgatac gacccatccc agtgtctgct tttagatgaa tcgaaagagg
    24241 cgtctctagc ttacttttct ctaaaacagc agctgctgct tgcaaccatt cccgtgtacc
    24301 tgcagttact gataaatcat gagcaacagc tacagtcagt gcgtcgtaag gaaccatatt
    24361 caagataagg atgggctgag taaatccagc ttctctcaac tcgactcctt catctaaatt
    24421 tgaaacacaa aagcccgaag ctccacctgc aactgctgcc tcagctgttt caatagctcc
    24481 atgaccgtat ccgttcgctt ttactactgc aaacaattct tttccttgcg gaagtcttgc
    24541 aacttcattt gctacatttt cagttatagc ttttttatgt atcactgctt tcgttgggcg
    24601 gtgccaagcg acaaccatcg tttttccttc tttctagtct tccaaaataa tttgtgcaaa
    24661 agctgtttca tttgtatgag taatactgac ccatacattg ccttcatgag gtgaacgcgt
    24721 cacaacaggc tgaccatttt cattttttaa aatctcgaca tcttgaaagg taacttttcc
    24781 gatcccagtt ccccaagctt tagagaaagc ctctttgcaa gcgtaacgcc cgcctaaaaa
    24841 ttctacttgg cgatgaaaag gcaaggactg gaatagtttc atttcatcag aggtcaagat
    24901 tcgtgcaatg aattttggtt tttcttcgat cagccgagtg atcctaggaa gttcgactgc
    24961 atcgatccca attcctttta tcatatgctt ttctcttttc aataaattta gaacactctc
    25021 attctaccaa attataacca ttttgccaca aataagtcca caaataacaa aaatgaaagt
    25081 ttctcttaaa aaagagaaac tttcatttta tgtttaatcg ttatttgaac ggattacaaa
    25141 tccgcgtttt ttatcattgt gacgtttacc gccatctttt tggctgttat tatttttgct
    25201 ataaccgcct tttgatttgt ttccacgata attaccgccg tcacgacgat tacggttgtt
    25261 tccgccacca cgtttgccat ttttattgta tccttttttc tgcattggca atgggcgttc
    25321 tggtgtgatc ttcactggca ctgcatcagc tggatctttt gctgtcgttt ttagcaaaag
    25381 tgcaactaaa tcttgtgcag aatattcttc taataattta tctgctgttt gcaggtattt
    25441 atctagacca ttttctgcaa gttttgtttc gatttgttca actgcagcac ctaattgacc
    25501 tttgaatgct tctttttcgg ttggtggacg taaagtcgtc atccgttttt tcgtcaagtt
    25561 ttcaatcaca tgcaagtagt ccatttcatt tggtgtaacg aaagtcacag acataccgcc
    25621 ttttccggca cggcctgtac gtccgatacg gtgaacgtag ctttctggat cttgagggat
    25681 atcatagttg tacacatgag tgacacctga aatatctaat ccacgtgcag caacgtctgt
    25741 cgctacaagg atatctaaat gaccgctctt aaatgaacgc aatacgctca ttcgtttttg
    25801 ttgagacaaa tcgccatgga ttccttctgc acggtaaccg cgtgcttcta atccgcgtgc
    25861 caattcgtct acacggcgtt ttgtacgtcc gaagacgatt gttaattcag gtgtttgaac
    25921 atcaaacaag cgagtcatga tatcgaattt ttcgtattct tttgcgcgga catagtattg
    25981 atcgatcaag tcagctgtca tttctttcgc tttgatcttc acatgatgtg gatttttcat
    26041 gaatttaacg ccaatattct tgatagctgg cggcatcgtt gctgagaaaa gtaatgtttg
    26101 tctttgatca ggaacttgtg aaatgatttt ttcgatatct tccaagaagc ccatattcaa
    26161 catttcatct gcttcatcca aaactaatgt ttgaacggtt ccaagtttca atgtgtggcg
    26221 gttaatgtgg tcaagcatac gtcctggtgt acccacaacg atatgtggac gatctttcaa
    26281 gccacggatc tgacggccaa tatctgctcc gccgtaaacg gcttgtacac gtactttttt
    26341 gtcgcgccct aaacgatata attcttcttg tgtttggata gctaattcac gtgttggtgc
    26401 gataactaaa ccttgtaatt cgtgacgatc tggatcgatt ttttctaaca ttggtaatcc
    26461 aaatgcagct gttttccctg tacctgtctg tgcttgtcca ataacgtctt ttccagctaa
    26521 tgctagtgga atcgtttctg cttggatagg cgttgcttct tcaaaaccag cgcgttcgac
    26581 tgatttcaac aattctggtg ataattctag ttctttaaat ttcaaatgaa tcctcctata
    26641 tggttgttaa ctgaggatgt agcttttttg ctaccccgtc gatttgttcg gtcaaagtgt
    26701 attgtttctt tttgacagtg gccacgcacg tttttatcca ttcttagccc ctgtggcacc
    26761 accgaaaaat cactttgcga agtttaggca ttagaaaagc caaacgggtc gcgttcagct
    26821 ttatcattat tatgacatct ggcttcaaca agttgtttct atggcaaaac cagtccaatt
    26881 tctcttcatc tttcggcaat aggttattct aacatagttt ttcgttttct gcaagtttac
    26941 agatttttat ttcatttgct gaagttgttc taccacttca aataagccca tgctgttgct
    27001 gccttttagc ataatactat ctgttgctgc aagattttgt ttgatagcat caatcaattt
    27061 attcttttct ttttcaaaat agaacacagg tagttggggg tatttttggt tcaattcttc
    27121 ttttagtgca tgcatttgtt ccccataaag gaaaatcatg gcgaatttct caggatcgat
    27181 atgttcactc atttgcgcat gcatatttaa agactctttc cctaattcca gcatatctgc
    27241 taacacagcg attctgcgtc cttttgttgg cagattttta aaagtatcta ataccagtcc
    27301 catggctgtt ggatttgcat tgtagacatc gctcaaaata gctgcaccat ttcctgccgt
    27361 tagccattcg gttctgtttt gagtgataga aacatgggcg agcccttggc gtatctcagg
    27421 tatggacagt ccaaagaagc gcccgatcgt atacgcaatc aaagcattcg ttacattata
    27481 acttccaggc agcgggatcg taaatgtttc tccttctact acaaaatcgg tttgtgcttt
    27541 tccttttgct gtcacttccg catgaacatc gccatttcca ataccgaatg ttgtgactgt
    27601 ttgtactaat gttgccgtta aaggtgtcag caacggttca tctgcgggca caacgagtaa
    27661 tccgtctgat acaagtccgt cagtgatttc cattttggct ttagcgattc cttctcgcga
    27721 cccaagattt tctatgtgtg cttcaccgat cattgtgatt gctgcagctt ttggttgagc
    27781 cataagtgaa agttccgtga tttcattggc gtgatccatg cccatttcca aaacgatctt
    27841 ttctgtatct tggggcatgt gcaaaatcgt gtaaggaaga ccaatattat tgttgtaatt
    27901 tccttgcgtc ttatatgttt tgtatttttc agacaagaca gacgcggtta gatctttcgt
    27961 ggtggtttta ccattgcttc ctgtaattgc aacgacttct gcgcctactt tttccaagta
    28021 ataggttgcc aattgctgca tggctttcaa aggatctgct acaggaataa ctggaaaatt
    28081 ttttggagct tgttcaacag gcaagctcca aaatgaagca gatgctccgt ttgtgatcgc
    28141 ttgctggata aattcatgtc catctcgcgt accagcaaga ggaacgaata aacttccttc
    28201 ggttatcttc cggctgtcaa attcgacatt ggtaatcttt gtcagtgcag agcattcgct
    28261 gcccacggct tgtgcaattt cttgtattgt tagattcatt gagatctctc ctaaaaaagg
    28321 ttgtgaaaaa agcgtttggc ctaaacggcg ggttcgcatg actctcactt ccaagtaaat
    28381 ttctctattc gtatttagaa gctagaatga acgcggttac gcaatcactc cagcctcttc
    28441 tttatttctc tttattcatt tttttcaaaa aaagattgac gctgatggta tcgattcatt
    28501 cctaactgga tcagttcttc aatcaaatca ccgtatttca agcccatatt ttcccataag
    28561 agtgggtaca tactgaactc cgtaaatcct ggcatagagt ttaattcatt caggaataat
    28621 tcatttttat ttgtcaaaaa gaaatcgcac cggctcaatc cgcttccacc taacatcgtg
    28681 taagctaact tcgcgtactc ttgcgctttt tgataaactt cttctggcac ttcggctgga
    28741 atctgcattt cgattttatt attgatatat tttgcttcat aatcatagaa tgctacgtct
    28801 tttacgactt caccaggcaa agtcgtccga acgtcttcat ttcctaatac agcaacttcg
    28861 atttcgcgcg cttcaattcc ttgttcaacg attgctcgag aatcatactg ataggctgtt
    28921 gctaaagcat tttgcagctc ttctcggttt tctgcttttg taatgccgac actagaaccc
    28981 atattcgccg gtttgacaaa catcggataa agcaaagaac cttcacattg atcaaatact
    29041 tttttaggat tttctttcca ttgattctta agtactggta cataaggaac ttgcggcaca
    29101 ccagcagctt gtaaaatata cttggtcatg attttatcca tggcacatgc actggtcaat
    29161 acgcctgcgc cgacataagg catattcaat gtctctaaga agccttggat cgttccatct
    29221 tccccgtttg gcccatgtaa aactggaaaa acgatggctc cttcttcttt gatttcgccc
    29281 ggattgatca cttttcctgt aaagccttcc tctgtctgtc cacttgggtc ccatgaaaga
    29341 tgcaagacat ctttgctggc aggtttttct gttagtaatg gacctttgac ccattgtcct
    29401 tcttttgtaa taaatacgag ttgaacttgg taataattat aataaatggc atttaaaact
    29461 gaaaatgcgg aaagaatgga cacttcatgc tctgcgctgc gtccgccata tagtaaagta
    29521 atcttcaaaa gattgtcctc ctgtattcta aatcgttcag tgatttactc tcatatttta
    29581 gcacaaaaca aaaataaacg aataacttat ttataaaaaa acgagccagc ttctaaaaga
    29641 acgagcgtca tttatttttt gtcagctaga ttatttgtcc cgttttttat aaaaagttat
    29701 gataaagaaa aggagggatg atgatggcgc aaacaaatcc acgcagccgc ttggcagaaa
    29761 tcattcgagt gttcatcaat tacaatgtag tgccaaactt tgtacagcaa aaaaatcccg
    29821 aacaggtaaa aaaagccttt gaagaactag gccccacctt catcaagata ggacagatgc
    29881 tttctgttcg agaagatttg ctttcttctg ccttcactca aacattcaaa acattacaag
    29941 atagtgtacc tagcgacaca ttttcaacgg tcaaaaagac gatcgaaaca gaactttctc
    30001 tttcattatc agatatattc gatgattttt caaaatcgcc ttttgcctct gcttctatgg
    30061 ggcaggctca tcgtgccaag ctgaagaacg gtgacgcggt agtcgttaag atccaacacc
    30121 ctaacatcgc cgaagagatc cgacttgatc ttcaactatt tgaacgagcg atccctttga
    30181 tcaaatacat tcctgaaact agcgtagttg atctaaaagg ggtactccaa gaagtaaaac
    30241 gttccttgat caatgaaatg gacttcttaa aagaaagtca aaatggcgaa caattctatc
    30301 aaaagaacaa tggctggaaa gagatccgca gccccaagat ttatgacgct ttttgttcga
    30361 aaaaagtgat cgtgatggaa gaaatgtctg gtaaaaactt gaatcattta atgaacgccg
    30421 aaaacaaaac tgaaactttt atcactggaa ttcaaaacaa gcagctgaaa caagaagttg
    30481 caaagctttt agttgagaac tttatgaaac aggtgtttga tgatggtttt ttccatgctg
    30541 atcctcatcc agggaatctg ctattccatg tactaacaaa agaagaacag acacaagcta
    30601 gccgaaaaac tgaaacagtc catgaaaaag agttcggttc ctttgctttt cgagcctcca
    30661 cttctgcaga agaccctgtt gcgccatata ccatcaacta catcgatttt ggtatgatgg
    30721 ggcatctttc tgcaggactt aggcaaaaac tcacacaagc cgttttagcg ctgtatacga
    30781 aagatgctta tcgaatcgaa aaagcggtcc ttcgactttg tcagcaagaa ggctcgtttg
    30841 atgaaagtcg gttccaccaa gaactgacaa gttttcttga acagtattat gattctccta
    30901 ttgatgaaat caacttgcaa gaagtattca cacatgtcgt gacgatttgt catcaaaata
    30961 atctgcagtt cgatcgtgat atcacgcttt tgctgaaagc atttgggaca ttagaagggg
    31021 tgatccgcgt cctcgatcct gaagtttccc tcatggaagt cgcttcgcct tttgctcaac
    31081 attattttct cactcatctg gatgtagaag atacattgaa acaaagcgga ttagacttat
    31141 tggaagggat gaaggctgcg cctaagatcc ctcaacagct ccaccatcta ttagaaatgt
    31201 ggacttcggg acaaggaaaa gtcaatttgg aattgaaaaa gcaagacaaa ttattgagca
    31261 ggatcgaaag tatgatcaat cggcttgttt tcggtatgat tttagctgca ttgatcgtag
    31321 ggtcctccct gcttgtccaa gcagcacctg tagaaaacgc agaggtcgtt tccttattag
    31381 ggatatttac gtatgctatt gccgcattcg tcattatttt tttggcaatc gatgcattga
    31441 tccaaatgta taagaagagg aaaaagtaga aaaaggttgt gacagaaaga atcagcttca
    31501 agaaataaga aggggtttta cataaattgt ttctcaaatt tttgtgaaaa ttcggcttat
    31561 ttttcgaaga ggctacttct gaaacaccga gaataaaaaa tgtgaaaaag ctgttctgca
    31621 cccagtaata agaacaatca aaaagggaac atggaacaca tttatcctgt ttccctgttc
    31681 cctttttgat gtattcgagc ataaataaaa taccttcgtt acctactatc ttctgtactt
    31741 ggtaaacctt agtcaattgc cttttttaac gcttgacgaa aagacatatg atctttctcc
    31801 gtaaagggtt ttggaccctt tgtccttatg ccagcttttc ttaactttcc actatgataa
    31861 cgttgttcta gaagcgtgcc gatattcgct tcgtcaaatt cttttccaga gtgatggaac
    31921 actcgcacac ccttcggcaa tacaagtgaa gctaaaccat aatcttgcgt gatcaaaaag
    31981 tcattcttct taaccatacc gacgatttta taatccgcac tatcagctcc gcggtctacg
    32041 tagacgattt ggacatgatt agcgtattct ttggtcgtat aatggtccac actggtcacg
    32101 ataaccacgt caagaccata ggattctcct aattttatga catcttcttt tactggagaa
    32161 ccatctccat caatgaatag cctcattctt tttcgccttc ttccataaat gataaacagt
    32221 tactaatccg ctcagtaaaa gaccaacatt gatccatttt gccaagataa gcagttgtac
    32281 actttctatc ggcactgatt catggttcag cgtgatcaat aatagatgga ttggctctcc
    32341 catttctttt gctaaaacaa acaaaagcgt catcaataaa aactggtgga tcacactccc
    32401 tttattttga gtagtatact tgacaacgat ccggcgatcc attggaatag ctgtatcagt
    32461 ttgataatat tccttattta gccagctgcc tgttactacg tgccagaaac taagcagaaa
    32521 gacacctatc gcaaaaccat ttggaatcaa gaggaccaat aaagaagaac tcagtccaag
    32581 caattgcata gccaatggag accatgttaa acgtttcaaa atccatggtc cgactatttt
    32641 tgctgcaatc aagcctgctg cataaggcaa atacataaaa atcgaaagtt tttctaatcc
    32701 gtacagtatg ccaacataaa aactgctaaa taaaaaaagg aaattcccta gcattccatt
    32761 tagaaaagta aacagattta gccagcttgg caacttccat tccttcttct ggtgtcctaa
    32821 aacaaacacg aacaagatga acagcagcaa gaaaccataa atagcaagat caaaaagcct
    32881 ttcatcgaat aacaatcggg catttctcaa aagaaacaat aagccaaaga atatcaaaaa
    32941 taaaacaagc tctcgcgtag ctaccagatt tcttttgatg tctttgaaat ccagttcgta
    33001 acgcggataa tgactaaccg tatggtaggc catgacgtaa aaaagtgtat aaatagtaaa
    33061 gcttgctgca atctgagtcg gtgcaggcaa ctcaatcgct tgatacagga gaaatagcca
    33121 gatagctgca aataagtact ttttcccatc catattgata aaaccttgcg tcttttcgtg
    33181 aaaattgacc gtggtattag caggtacaag ccatgctgca ctcagcccta gcaaaacact
    33241 tgaaaaataa aataaaggaa aataaaatac gcctaatatt ccaagcaatg aaccagcacc
    33301 accaatcagc agagcgatca tcagcaacgt gtaactatct agaccaaatc gtagactgtt
    33361 gactaaaaat agtccagtca tccgaaaggc ataaaacagc gtaaatggca aaatcgtcag
    33421 ccaattatgc gagtctgtta gtcccaaaaa taaaagataa ggaataaaaa taaccgtgtt
    33481 acttaagata aagggcgttg tttgtgtaaa attacgccca aatcgtttga gtttttcgaa
    33541 catgacaatg gctccttaaa cagtcttata actaatctgc tcttcgattt tagcattaaa
    33601 ggggaaataa accaatttta cagaatatat tcttgtaaaa aaaaatacag aaagaagcat
    33661 aagcttcttt ctgcattttt acggtacttg attactcaag cagacaagcc cttctccaca
    33721 aaagtcttgc ccgacacccc tgcatccggc aggtgataaa gatagaaatc aattatcctg
    33781 tgcctccaaa atcaattagg agctctgtaa accagagtcc ttctcgaccc gtcgcataaa
    33841 tttactttaa cacagttcct atgtttatgc gagagctttt ttcattttat aatcgaccat
    33901 ttgaaatcct tgaccgatat ctgaaataag ttcttctgta atctcaaatc cttcatgctt
    33961 ataaacctcg attgcttgat gattcccctg attcactcgc aaaaagatct ctctttcctt
    34021 tgtttgcgca gcaagttctt cataccagcg aaaaactgaa gaagtcaatc cttgaccacg
    34081 tactttattt agcaaatata atttacttaa ataaattttt ccatttatct cttcataagc
    34141 agtgtaaccg acagtctttt cttcgctttt taataaaaag taggacacac cttctgtcag
    34201 ttctttttga atctgttttt ttgactgata agttttcagc atgtaatcca cttgtttttg
    34261 accaatgatc ggcacaaaag cttccggcca tacttcttgt atgactgcca tcagctcatc
    34321 cagttcattt tctgttactt ttattttttc catacaccca cgtccttttt tacattataa
    34381 atgaccttgc gcttgatgga ataaaaagag catgatcatg ctatacacac ccaccgacca
    34441 tggtttcaag aaaatcacaa tctgcataaa ttccctaaaa gataatttcg tcaaaccagc
    34501 tactagacag atcaagtcgt ctggcgcaaa gggaagcagc aaggtaacaa ctagtacttt
    34561 tttaatatct ttcgtttttc ttgtcagcaa ttcatcaaat ttctgatact tttttggcga
    34621 taaaataagc tggacaaagg ctacgccata gtaacggaca agcaaaaatc cgattgcttc
    34681 cccaatcact aggccaatac aactatataa aagcccaaat ccattgccaa acatcagcat
    34741 gcccgctact gaggagatcc ctcccggcaa aataggaatg atcacttgga tcacttgcaa
    34801 cagaacaaat atcatcactg catacttgcc aaattgctgg ataaacgctt gcaaagacgt
    34861 gctcgatcga aaaatcccat gatgataagc ataaagaatc aacacaaaga aaaaaaacag
    34921 accaataatg ggcatgatct gaagacattt ttgtaaaact gatccttttt tagacatcta
    34981 tattccccac atcttttttc atttattaaa actgcctcc
//
LOCUS       DAFEOM010000021        34636 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.022, whole genome shotgun sequence.
ACCESSION   DAFEOM010000021
VERSION     DAFEOM010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.022"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            591..1280
                     /locus_tag="LP524_001205"
     CDS             591..1280
                     /locus_tag="LP524_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2234270.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="LP524_001206"
     CDS             1267..2475
                     /locus_tag="LP524_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL2234271.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="LP524_001207"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="LP524_001207"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBL2234272.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="LP524_001208"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="LP524_001208"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBL2234273.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="LP524_001209"
     CDS             complement(6578..7273)
                     /locus_tag="LP524_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HBL2234274.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="LP524_001210"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="LP524_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBL2234275.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="LP524_001211"
     CDS             complement(8472..8663)
                     /locus_tag="LP524_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBL2234276.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="LP524_001212"
     CDS             complement(8684..9574)
                     /locus_tag="LP524_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBL2234277.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="LP524_001213"
     CDS             complement(9561..10328)
                     /locus_tag="LP524_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBL2234278.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="LP524_001214"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="LP524_001214"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBL2234279.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="LP524_001215"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="LP524_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBL2234280.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="LP524_001216"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="LP524_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBL2234281.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="LP524_001217"
     CDS             14899..15282
                     /locus_tag="LP524_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234282.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="LP524_001218"
     CDS             complement(15450..16208)
                     /locus_tag="LP524_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HBL2234283.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="LP524_001219"
     CDS             complement(16226..17044)
                     /locus_tag="LP524_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBL2234284.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="LP524_001220"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="LP524_001220"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBL2234285.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="LP524_001221"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="LP524_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBL2234286.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="LP524_001222"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="LP524_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBL2234287.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="LP524_001223"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="LP524_001223"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBL2234288.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="LP524_001224"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="LP524_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBL2234289.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="LP524_001225"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="LP524_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBL2234290.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="LP524_001226"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="LP524_001226"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBL2234291.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="LP524_001227"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="LP524_001227"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBL2234292.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="LP524_001228"
     CDS             27086..27385
                     /locus_tag="LP524_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBL2234293.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="LP524_001229"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="LP524_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL2234294.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /locus_tag="LP524_001230"
     CDS             27973..28209
                     /locus_tag="LP524_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBL2234295.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="LP524_001231"
     CDS             28352..30325
                     /locus_tag="LP524_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBL2234296.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="LP524_001232"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="LP524_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBL2234297.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="LP524_001233"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="LP524_001233"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBL2234298.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="LP524_001234"
     CDS             33204..34496
                     /locus_tag="LP524_001234"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBL2234299.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10554 a         6733 c         6844 g        10505 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagac caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgttaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggattaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggcggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccgtc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagactat gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atgggaaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatct
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaattct tgaaaactat
    25441 atcgaacatc agcgcgaagt catcacaaga cggacagtct ttgaaaaaga aaaagcagaa
    25501 gcaagggcac acattttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaagcaaaag cgacgatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgcacagc caaaggaata ccaattatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggttagagg aatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aagatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaatag ccatcaagac taattgtgta agctagcaag
    26881 aatgaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataatcag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattggatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaac gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atatcgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaacact gtttattcag aataagggac tgtgacaaaa accgtgtcac
    34621 agtccctttt tccgaa
//
LOCUS       DAFEOM010000022        34589 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.023, whole genome shotgun sequence.
ACCESSION   DAFEOM010000022
VERSION     DAFEOM010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.023"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            212..604
                     /locus_tag="LP524_001235"
     CDS             212..604
                     /locus_tag="LP524_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL2234300.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="LP524_001236"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="LP524_001236"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HBL2234301.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="LP524_001237"
     CDS             3165..4775
                     /locus_tag="LP524_001237"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HBL2234302.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="LP524_001238"
     CDS             5067..5936
                     /locus_tag="LP524_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HBL2234303.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="LP524_001239"
     CDS             6101..7372
                     /locus_tag="LP524_001239"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBL2234304.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="LP524_001240"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="LP524_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBL2234305.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="LP524_001241"
     CDS             8763..9026
                     /locus_tag="LP524_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HBL2234306.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="LP524_001242"
     CDS             9471..9920
                     /locus_tag="LP524_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL2234307.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="LP524_001243"
     CDS             9977..11395
                     /locus_tag="LP524_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL2234308.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="LP524_001244"
     CDS             11395..12255
                     /locus_tag="LP524_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBL2234309.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="LP524_001245"
     CDS             12429..13202
                     /locus_tag="LP524_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL2234310.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="LP524_001246"
     CDS             13349..13714
                     /locus_tag="LP524_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HBL2234311.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="LP524_001247"
     CDS             complement(13862..15070)
                     /locus_tag="LP524_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL2234312.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="LP524_001248"
     CDS             complement(15089..15367)
                     /locus_tag="LP524_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234313.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="LP524_001249"
     CDS             15521..16216
                     /locus_tag="LP524_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBL2234314.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="LP524_001250"
     CDS             complement(16658..17455)
                     /locus_tag="LP524_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HBL2234315.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="LP524_001251"
     CDS             complement(17452..18636)
                     /locus_tag="LP524_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBL2234316.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="LP524_001252"
     CDS             complement(18661..19908)
                     /locus_tag="LP524_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBL2234317.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="LP524_001253"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="LP524_001253"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBL2234318.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="LP524_001254"
     CDS             21888..22202
                     /locus_tag="LP524_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBL2234319.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="LP524_001255"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="LP524_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBL2234320.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="LP524_001256"
     CDS             22887..23531
                     /locus_tag="LP524_001256"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBL2234321.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="LP524_001257"
     CDS             23546..23875
                     /locus_tag="LP524_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HBL2234322.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="LP524_001258"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="LP524_001258"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBL2234323.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="LP524_001259"
     CDS             24863..25687
                     /locus_tag="LP524_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBL2234324.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="LP524_001260"
     CDS             25680..26027
                     /locus_tag="LP524_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HBL2234325.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="LP524_001261"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="LP524_001261"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBL2234326.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="LP524_001262"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="LP524_001262"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBL2234327.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="LP524_001263"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="LP524_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBL2234328.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="LP524_001264"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="LP524_001264"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBL2234329.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31657..32451
                     /locus_tag="LP524_001265"
     CDS             31657..32451
                     /locus_tag="LP524_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2234330.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="LP524_001266"
     CDS             32441..34483
                     /locus_tag="LP524_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2234331.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT        11118 a         6130 c         7260 g        10081 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcgcgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttata
//
LOCUS       DAFEOM010000023        33016 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.024, whole genome shotgun sequence.
ACCESSION   DAFEOM010000023
VERSION     DAFEOM010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.024"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(63..1322)
                     /locus_tag="LP524_001267"
     CDS             complement(63..1322)
                     /locus_tag="LP524_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HBL2234332.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(1493..1939)
                     /locus_tag="LP524_001268"
     CDS             complement(1493..1939)
                     /locus_tag="LP524_001268"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBL2234333.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            complement(1950..4163)
                     /locus_tag="LP524_001269"
     CDS             complement(1950..4163)
                     /locus_tag="LP524_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBL2234334.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            complement(4396..5160)
                     /locus_tag="LP524_001270"
     CDS             complement(4396..5160)
                     /locus_tag="LP524_001270"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBL2234335.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(5161..6108)
                     /gene="prmA"
                     /locus_tag="LP524_001271"
     CDS             complement(5161..6108)
                     /gene="prmA"
                     /locus_tag="LP524_001271"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBL2234336.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(6121..6609)
                     /locus_tag="LP524_001272"
     CDS             complement(6121..6609)
                     /locus_tag="LP524_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HBL2234337.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(6575..7246)
                     /locus_tag="LP524_001273"
     CDS             complement(6575..7246)
                     /locus_tag="LP524_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBL2234338.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            7384..8670
                     /locus_tag="LP524_001274"
     CDS             7384..8670
                     /locus_tag="LP524_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBL2234339.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKSAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            8742..8936
                     /gene="ssrS"
                     /locus_tag="LP524_001275"
     ncRNA           8742..8936
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="LP524_001275"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            9014..9298
                     /locus_tag="LP524_001276"
     CDS             9014..9298
                     /locus_tag="LP524_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234340.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            9371..9850
                     /locus_tag="LP524_001277"
     CDS             9371..9850
                     /locus_tag="LP524_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBL2234341.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            10073..10216
                     /locus_tag="LP524_001278"
     CDS             10073..10216
                     /locus_tag="LP524_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HBL2234342.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            10794..12377
                     /locus_tag="LP524_001279"
     CDS             10794..12377
                     /locus_tag="LP524_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL2234343.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(12814..13998)
                     /locus_tag="LP524_001280"
     CDS             complement(12814..13998)
                     /locus_tag="LP524_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBL2234344.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTMMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(14023..15030)
                     /locus_tag="LP524_001281"
     CDS             complement(14023..15030)
                     /locus_tag="LP524_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL2234345.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(15202..15561)
                     /locus_tag="LP524_001282"
     CDS             complement(15202..15561)
                     /locus_tag="LP524_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234346.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(15533..15904)
                     /locus_tag="LP524_001283"
     CDS             complement(15533..15904)
                     /locus_tag="LP524_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HBL2234347.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(15921..16223)
                     /locus_tag="LP524_001284"
     CDS             complement(15921..16223)
                     /locus_tag="LP524_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234348.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(16240..16710)
                     /locus_tag="LP524_001285"
     CDS             complement(16240..16710)
                     /locus_tag="LP524_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBL2234349.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(16685..16963)
                     /locus_tag="LP524_001286"
     CDS             complement(16685..16963)
                     /locus_tag="LP524_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBL2234350.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(16960..18009)
                     /locus_tag="LP524_001287"
     CDS             complement(16960..18009)
                     /locus_tag="LP524_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HBL2234351.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(17966..18895)
                     /gene="tadA"
                     /locus_tag="LP524_001288"
     CDS             complement(17966..18895)
                     /gene="tadA"
                     /locus_tag="LP524_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HBL2234352.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            19027..20781
                     /gene="ade"
                     /locus_tag="LP524_001289"
     CDS             19027..20781
                     /gene="ade"
                     /locus_tag="LP524_001289"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBL2234353.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(20879..21541)
                     /gene="trmB"
                     /locus_tag="LP524_001290"
     CDS             complement(20879..21541)
                     /gene="trmB"
                     /locus_tag="LP524_001290"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBL2234354.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(21611..22393)
                     /locus_tag="LP524_001291"
     CDS             complement(21611..22393)
                     /locus_tag="LP524_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HBL2234355.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(22597..23808)
                     /locus_tag="LP524_001292"
     CDS             complement(22597..23808)
                     /locus_tag="LP524_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2234356.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(23801..24538)
                     /locus_tag="LP524_001293"
     CDS             complement(23801..24538)
                     /locus_tag="LP524_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2234357.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            24676..25110
                     /locus_tag="LP524_001294"
     CDS             24676..25110
                     /locus_tag="LP524_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HBL2234358.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            25103..25447
                     /locus_tag="LP524_001295"
     CDS             25103..25447
                     /locus_tag="LP524_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBL2234359.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            25627..26637
                     /locus_tag="LP524_001296"
     CDS             25627..26637
                     /locus_tag="LP524_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL2234360.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(26706..27650)
                     /locus_tag="LP524_001297"
     CDS             complement(26706..27650)
                     /locus_tag="LP524_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL2234361.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(27634..30354)
                     /locus_tag="LP524_001298"
     CDS             complement(27634..30354)
                     /locus_tag="LP524_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL2234362.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(30351..31571)
                     /locus_tag="LP524_001299"
     CDS             complement(30351..31571)
                     /locus_tag="LP524_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HBL2234363.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(31852..32697)
                     /locus_tag="LP524_001300"
     CDS             complement(31852..32697)
                     /locus_tag="LP524_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL2234364.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT         9825 a         6784 c         5719 g        10688 t
ORIGIN      
        1 atatgaaaaa aggaagatca gcactgtttg atcttccttt ttttcgataa gtttatgcta
       61 atctaattct tgtaaagtag cgctcggcta ttcggataag tcggaaactg attgaatgga
      121 atcgtccaat cttgttcagg tacagagaac tcaaacttat ttactagctg atcggaaaac
      181 acacgtagac tatgaagagt gatccattct cctgcacagc gatgcatttg tcgaaaattt
      241 cccccgcctt gtgcaatcat ctcatattct tctttatagg aaatatcttt tgctttccct
      301 acataccgtt tgatcataaa ggaatctggc gcttcaactg ttcgttcatc atggtctgtt
      361 ccatataaat ctaaaataac ccaactgcct tctggtatac gataaccatc tacttctact
      421 tctttcagcg aaatcgcagg tagcattggg aagaaaggat aatagcgtct catttcttga
      481 ataaacggat cttgtaacgt cgaaaagtca ttttttaatt gctgatacaa atctggtcta
      541 ctgaaaagtg catgtcccat caatgccgcc cacacagtta gagcaaccgt tggacggatg
      601 atgttcaata attcaacagc tgccacttcc agaggtaaaa gctgaccatc taaatctgtg
      661 gcattggcaa aagcatataa tgccacattt tcttttccag ccacaggatt ttgtcgagct
      721 tcttcaatca agccttgcgc ccatttttct gattttttgc gattttcgac accttttatg
      781 tgatcaatcg gagaagtaaa cgttccgctg atcatagaga tttgatattc tgctaatttt
      841 tcgacctcat ctttagaaat cgctgataaa ttgatacctg cccactcgca aatagatgta
      901 aagaaaacca tcttacttaa atcaaatagt tcaaattgac catgctgtgc ttcaagtgct
      961 tgtgtcagat tcttatctaa gatacgatga taatcttcca ttctttccgg tgtcattaga
     1021 tccataaaaa tatttttccg atggtgatga gcagctccat ccaaagtctg tactccacct
     1081 tgaccaaaaa gtgtcttcaa tactaatttt ggcatagcac cttttcgttt gaaattcctt
     1141 ggatcataaa atacttttgc tgctgatgat ccgtagatcg tgatagcttc tttattgaaa
     1201 atctttgctt tgacgacggg agcgtccgct tcatggcgta actcctccaa catgttgtat
     1261 cctttttgat acaatttttt taaatctgtg attttgatat ctactactgg tacttctttc
     1321 atcttcagca acctcctata aaaaataact tgtataattt aagcttaact ttttttccga
     1381 ctgctgacaa aagaagcgct ttttgcagac aaaaaagcca cttaagttag agtttatcag
     1441 tctaacttaa gcgacgtgtt tcatagtttt tactagtgaa aaccagatta ctttatttgt
     1501 ttttagaatc aataatgatc gttacaggtc catcattgac gagagaaacc gacatatctg
     1561 ctccgaattg tccggtagcg actgtgatgc cgcgacttct gattccttca ttgaatgatt
     1621 cgtataaagg aatagctgtt tctggtcgtg cggcttccac aaagcttggt cgatttcctt
     1681 ttttcgtatc cgcaaacagt gtgaattgag agatacttaa aatttgtcct cccacatctt
     1741 ctacactaat attcattttt ccttgttcat cttcaaaaat acgcatttgt gcaatttttt
     1801 ttaccagata gtccacatct ttttgcgtat cggtttcatg aatgcctaat aaaacaagta
     1861 ggccgtagcc aatttttcca atttcttgct gatcgactga cacagcagct ttacttaccc
     1921 gttgaacaac tgctctcata tctgtcctcc tatccattcg tccggcgtac actgtagaca
     1981 tcaggtactg ctttgatttt atccacgata ctctttaggt gggataaatt ctggatacca
     2041 accgtcaaat ggatcgtcgc cattttatct ttattcgtcc gtgcttcaac gctgttgaga
     2101 tttttcgtta gcgcattgac tgtttgaagt acatcattca ataagcctga acgattatag
     2161 ccgtaaattt ccaaatctgc atcatactct tttcgtgtat tcgatgtatc ttcccattcg
     2221 acttcaatca aacgttctgc tacatttggt ttgtcaggct gaacattcgg acaatctcga
     2281 cgatgaatgg atatccctct gcctttagtg atataaccaa cgatatcatc accaggaatc
     2341 ggattacagc agcgactgat acgaattagt aagttttcta ccccttgaat gacaacgcca
     2401 ccttcatgac gtactttcat tttgtcaggt tcttttttcg gctgattcat gatttcttct
     2461 gcttcttgct tttgctgctc gattttttgt tctttacgtt ctttttcagt cagacgattg
     2521 gccatcgtca aggggctaac ttctccatac cctacagctg catagagatc atcttctgtt
     2581 tgataattaa aacgatcgag tgcttcttgc agtttgttct tcgtcaaaat atctttaggc
     2641 gtaaatccta gatctgtaat acatttgacc acggattcgt ggcctttgat cacattttct
     2701 tctcgatctt gggctttgaa gaaacgtttg atcttatttc ttgctttgct agtagcaact
     2761 aatttcaacc agtcgcgact tgggccaaat gaatttggag aagtcatgat ctcaatgata
     2821 tctccgtttt tcaatttgta atcaagctgc accattttgc catttacttt tgcaccagtg
     2881 gttttgttac cgatatctgt atggatactg tatgcaaagt ccaatggtcc ggatcctttt
     2941 ggtaactcag taacgtctcc ttttggtgtg aagacgtaga ctttatcact aaagatatct
     3001 ccttttacgc cttccataaa ttcagaagca tcatagcttt cgtcttgaag ttcgagtatc
     3061 tcatggaacc agcttaattg tttcgtcata ccatctggtt ctactttttc attttttcct
     3121 tctttatagg cccagtgtgc agcaaccccg aattcagcga tttcatgcat ttcctgtgtt
     3181 cggatttgaa tttctaccgg atttcctgcc ggaccgatca cggtagtatg caaagattgg
     3241 tacatattag cttttggcat ggcaatataa tccttaaatc tgccgggcat tggtttccat
     3301 tttgtgtgga ttgctcctaa tacagcataa caatccttga ttgaatctac gatcactcga
     3361 atagctagca agtcataaat ttcgttgaac tgctttttct gatctttcat tttacgatag
     3421 attgaataaa tgtgttttgg ccgtccatag atttctgcaa aaatccccag ctcttctgtc
     3481 gctattcgaa tatcttcaac agtaccactt acgtattttt cacgttcttc tctcttcgtc
     3541 tgcatcaagt ggacgatacg gtaatactgt tttggattta gataacgaag tgctgtatct
     3601 tctaattccc atttgatccg gctgatccct agacgatgtg caagaggcgc atagatttct
     3661 aaagtttcct gagcgatcct tctttgctta tcttctcgca ggtgcttcaa cgtacgcatg
     3721 ttatgtagtc tatcagctaa tttgaccatg ataactcgta aatcttgagc cattgctagc
     3781 agcatctttc ggtggttttc tgctagctgc tcttcgtggg atttatattt tattttccct
     3841 aatttggtta cgccgtccac taacatagca acgtcatcgc caaattcttc tctcagatct
     3901 tctaaagtga tttctgtatc ttcaacaaca tcatgtaaga agcctgtagc cactgtatgg
     3961 ggatccattt ttaaatctgc taatatacct gcgacttgga taggatgaat aatgtagggt
     4021 tcgcctgatt ttctaaactg tccgtcatgt gcagcagtcg cgtattcgca tgctttctca
     4081 acaaatgcta catgttcggg acccatgtat tgactaacga gctttatgac accgggtccc
     4141 gttaaaattt cttcttttgt cacaggaatc gatccccctt tcttttttcg gtaaaacggg
     4201 aatcgggaca aatatctgtt tgataattgt cccaaaatcc gtgcagttat acgttttttt
     4261 acggaaatgt tcgcttttta aaagcttttt caaacgattt ttcgtatcca ttgtataaat
     4321 aattgtttct attatacaaa ttgttgacta ttttttcaat tctttctgtc gatactcttt
     4381 tattaatgaa gaacgttagc tgaaagaagg attcattaat tcccattgat agctagcagc
     4441 acttaataaa taaagaggtg ctgtttctgt acgaaggatt cttggtccta atccacaaag
     4501 tatccctttg tgttctacaa aataatcgac ttcatcgcgg gataatccac cttctggtcc
     4561 aaagacagct aagattcgac tatctggttt tgtttcacta agaattttgg ctgcatttga
     4621 tttttcgcct tgctttgctg attcctcata agcaaccaag accacgtcat aatcatctag
     4681 ccgctcaaat aaatcttgtt tcgtttccaa taactctact gatggcacga tctgccgatg
     4741 ggattgttcc gctgcctctt gagcaatctt ttcaagccgt tgttgtcgtt tcatacgttt
     4801 ttttgcatcc catttaacga cagaggctga tgccgggaaa ccaagaaaag cttgcgctcc
     4861 taattcagtg gatttctgaa cgatccaatc cagcttttcc cctttaggat agccactcgc
     4921 tatggtaatc gaacttggca gttccttttg attttcttct tttttaattt ctttcagata
     4981 aacagcctct tcagtaatct ccgtgatttc tgcaacaatg gctatttgat cattgaatgc
     5041 caaaaaaact tcatcctcgg gttccatcct cataacccgt acaatgtggt gatagttttc
     5101 cccttctaaa acaaaatagt cttttgattc atatgattct tttaaaaaat accgctgcat
     5161 ctattccacc tctggttttt tcaaaataat tgcataccag tctttttgct ggaaaatttg
     5221 atccacgata aacccttgtt ctaacatggc atccagaacc atttgcttct tctcttcaat
     5281 gattcctgaa acgatcagcg tgcctgtttg cttcagtaaa cgccaagcat ctgggatcat
     5341 caataagatg atatctgcta aaatgttcgc aacgattaca tcactttcaa tctcgattcc
     5401 tgtcaaaaga tcgttagctg atacatggac atctttggct atttcattca tatccatatt
     5461 ttctttcgcc gaacgaacgg ctacttcgtc taaatcaaag gcatagacat cttttgcacc
     5521 aagatatttt gctgcaatac ttagcacgcc cgacccagtt cccacatcta gcaatgtttc
     5581 accaccacgc aagactgttt ctagtgctgt caatgtcaag gaagtcgtgg gatgagtgcc
     5641 tgtgccaaat gccataccag gatctagtgt gataattttt tctagaggat cttctgtctg
     5701 gtagttttcc cagcttggcg caaccgtcag cattcgcgta atacgcaccg gatgatagta
     5761 tttcttccag gctgttgccc agttgctttc ctctacttca ctaactgtta ctttattttt
     5821 tccaatagct agaccaaact caggtaattg tgtgatacga tctttgataa aaggcaagat
     5881 ttccggtaag aaagtcgttt ccggaaaata agccatgact agtgcgcctt ctttcagcga
     5941 tgtaaaatca tttttatcta aaatctcacc atattgatcc gactgaaaat gttcaacatc
     6001 taaagaatct tcaatcgcga caccactcgc ccctgcctcc atcaaaatat tagcaactgc
     6061 ttcgactgct tcactttcag tttctacttt tacttcattc catttcatat ctgtcactcc
     6121 ttaataactt gggtacggta aataagtatc tgcttcattt tgctgtttta cttctactaa
     6181 ttctgctagt tgctgattat cccagtgaag ctcgaataca gcttcgtcat cgtcttcatc
     6241 caaaaaatca agcaaatcgc tttgcccttg agctaatact tcatttagat attccactaa
     6301 agaatcagct actgattttg ccaacccttt ttttccttca taagggatta ctgctagata
     6361 attgcgttca tcaaaaacag atttctgagg attaaaaaga agtacgccgt cttcaaattc
     6421 gataattggt tcttgcgaaa cgatcccttc tgcatcatca atctcaaatt gtgctttatt
     6481 ttctgcaaac aagcgaacaa cgatctcgat cgtatgattt tttgtatccc aatctaaagc
     6541 tacatcatag tctgtcactt gtttttcaat tatttcatct aaatatgtta atagatcctt
     6601 ctttttcatt ttctttcctc caaccccaat ctttctcgat ttggtttttt ctttgtaaag
     6661 agatataagg atttcccgac acaacaaaac gcaacggctt ttctgtccat acacctttgt
     6721 ttgggatacc gattctaggc aaagcattga tcttttttgg tgtctttttt ttctcaaaaa
     6781 ctaagcgtaa cgaactatct gctatagact gaccataaag ttcttgagga attgctaatg
     6841 cttccactaa ttttcccgga ccgttgctga tatctggacc agtttttccg ccacgatttt
     6901 tactcatttg atcaatcatt gcggcgggtt cgattgcacg tatcattact ccttgaggaa
     6961 tcccttctgg ctgagtaatg atgttcaaaa tccgatgagt atgcatcgtg taaaggtaga
     7021 tcgtacctgg tttttcatac attgcccgca cacgcggtgt tcttcttaaa ccatagctat
     7081 gggcggcctc gtcttctgga cctaggtatg cttccgcgtc tacaatatat ccagctaatc
     7141 ttccttctgg tgtgtcatgc tccaaataca tccccaataa atcccgtgcc acttctggcg
     7201 tcgttttatt ccaaaaaaat tcatctatct tttgtttatc cgtcattttt ctcacctact
     7261 ttatcataca caatattagt tggataagct atgaaaacaa aacaacagaa ggctgttctt
     7321 tctaggcatc ttgctaaata tgatataatt aagggacgta aaaacaagaa ggaggaaaat
     7381 cctatgcaac agccattggc ttatcgcatg agacctcgaa cgatcgatga agtcgttggt
     7441 cagcagcatc tagtcgggga aggaaaaatc atacgccgca tggtcgatgc tcgcatgctc
     7501 tcttccatga ttctatatgg acctcctggt actggaaaaa cgagtatcgc aagtgcgatc
     7561 gccggctcta ctaattatgc atttcgcatg ctaaacgccg ctacagattc aaaaaaggat
     7621 ctacaagttg tagcggaaga agcaaaaatg agtggtacag tgatcctctt attggatgaa
     7681 gtacaccgat tagataaaac aaaacaggat tttctgcttc ctcatttaga aagtggccga
     7741 atcatcttga tcggagcgac tactgagaat ccttatatta ccatcaatcc agcgatccgc
     7801 agccgtacac agatctttga agtcaaacca ttaacagaac aagacatcca gttggcagtc
     7861 gaacatgctt taagggataa agaacgcggc cttggacaac aagcaatcca gttagacgaa
     7921 gatgctcttc ttcacctttc gcgtgcaacg aacggcgatt tgcggagtgc attgaatggg
     7981 ctggaactgg ctacactatc gactccttca gataaagaag gaaggattca tcttacactg
     8041 tcgatcattg aagaatgtgt ccaaagaaaa gctcttaccc atgacaaaaa tggagatgct
     8101 cattatgacg ttatttcggc ttttcaaaaa tcgattcgtg gaagtgatgt ggatgctgca
     8161 cttcattatc tcgcccgttt agttgaggca ggtgatctgg caagtatttg ccggcgtctg
     8221 atggttatcg gttatgaaga tataggttta ggtaatcctg ctgctgcggc tcgtacagtg
     8281 aacgctgtat tagctgctga aaaattggga ttgcctgagg cacgtatccc actagcagac
     8341 gtcgtcgtcg atctttgctt gtcgccaaaa tccaattctg cctacatggc attagatgca
     8401 gcacttgctg atattcgaga gggcaaagct ggagatgttc ctgatcattt gagagatagc
     8461 cattacaaag gtgcgaaatc actcaaccgt ggcgtgggtt atcagtatcc tcatcatttt
     8521 gatcaggctt gggtcaatca gcaatattta ccagataaac tgaagagtgc tcagtattat
     8581 cagccaaaag atacagggaa atatgaacag gctctgggtc aacagtatta ccgtataaaa
     8641 gagtggaaaa agcatcctcc aaaaaactag acaagaaaac ggttctgttt ctgcgagctc
     8701 tctcattgct ctttttaaat tcatatgcta ctatagaggt gggaaatctg tgatgttcga
     8761 attgtcctct ttgtgtgttg accgaacatt tttattgata tcttgggacc cgtcttgtgt
     8821 cactgctggt aacatgcctt atcatttggt agttcgaagc tacgaccatg ggacccacct
     8881 gcttgaattc gcgggatcaa tactacagga caaacaacgg cattaacgga gattaccatc
     8941 ttttaaagaa taaagcaatt tgctcttggc aaattgcttt ttctatttgt tgcttatttt
     9001 ggaggatttg ttcatgatta ggattttaat gattatcgcc actttagtat tattattcgt
     9061 tagctattat ttatttaata aacaggatat cttttttgta ttgatcaaca aaaatgataa
     9121 aaatcaagga ttcctacaat tttacggagc tgcatatgct gttttaggcg taatgggtat
     9181 tttaacagct tttttcaatc aacgctttat tgccttggtc tttttactga tcgtaattat
     9241 tgtttctgcc accttcagta tccgatttgc aaaaaaaatt gctgaaccaa agcagtaaat
     9301 aattctcttt ttccctaaag tgttctacaa taaaagagta caatacgatt gacttgagga
     9361 gtgaatgctt atgttacaac aatacaaaaa aatcatggtt gccgtagatg gttctgatga
     9421 agctgaatta gcttttaaaa aggcggtcaa tgtagctatc cgaaataacg gagaacttct
     9481 attagcacat gtcattgaca cacgttcttt ccaaaccgtt tcatcctttg atggaatgct
     9541 tgcagaacaa gctactgaaa tggcaaagca aacattggca gattatgaaa gcaatgcgaa
     9601 gaaagctggc ttgaataatg taacctctgt cgtagaatat ggctcaccaa aacaaattat
     9661 tgccagagaa atcccagaag ataatcaagt agatttgatt atgttaggag ccacaggatt
     9721 gaatgctgta gaacgtttat ttattggttc tgtttctgaa tatgttatta gaaacgcagc
     9781 ttgcgatgtg cttgttgtcc gaactgattt agaaaaccaa ttgccagcta aagaccacac
     9841 caaagcttaa cacgtagacg acctgccaac tatcggcagg tttttttgca caaaaaaccg
     9901 tgataaaaac aaaagacgcg gcaagcactc atcggtcttt ataatgttta aaataaaggg
     9961 aaaacagtca atgagttcaa gtaaaataaa aaaaagtgaa caccagggcg attagtcgcc
    10021 ctggtgtggt ttaaaacgac ctaagcatgc caaagcttat tggttcgtca atgtggaagt
    10081 ttccgatgaa tctgatacga aaaaatatga agtatcgatg gcggataccg attttatcgc
    10141 caaactaaaa ttttatttcg ggttcgggta ccgcgagaag gcaagtgcta cattcggtaa
    10201 atcattcaag aattgatgaa aggctaccgc ttgccattgc aaccacaacg atactcctga
    10261 acgcgatgtt ggtgagattt ccaaagagaa gagctgttta atgcaacggt attaagcaaa
    10321 tggcaaaagg aagtcatgta gacaaagttt tttatcaaac accatatgat ttcgcctgaa
    10381 cgctggttta gctcagaata caatgatgtg gtccacgaga cattaccctc agtcagataa
    10441 aaaaagctct ctgacagaat tacacatcga taaatattaa gtaaatgtca gcgctagttg
    10501 aactttctga cactttcagg tacactggaa aagacaacta cacgaaggag ggaaaatact
    10561 atgatgcgca tgactaaaat aacaagtaat gaaaattacc aacgatacca tatcgcattt
    10621 agccatgcaa ttggttttac agttgctgtt gacgggacaa gtttgtccaa aacagttgaa
    10681 ttcacgtaaa atcaatggcc atctcaaggt atttttctca tgataatctg agtttaaata
    10741 acacaggcca tggataacca tggccttttt gagtggtctc aaggagagag atcatgatca
    10801 tacaagcaat ttctattaaa aaaacaatcg gcgggaatcc attatttgag gatttatcct
    10861 gtcaaattga agctggtgaa aaaattgcct tagtcggtat caacggaact ggcaaatcaa
    10921 cctttttaca aatcttgacc ggcagagaag gtgtcgacag cggtgttatc agtcgcaaaa
    10981 aaggcttaaa gatcgggacg gtcgaacaag aacttacagt ttccgaagcg accgtcaacc
    11041 actacttgct ccattctgcc gctgagatac aggacctcaa acagcaaatg agtcgctacg
    11101 aagcattgat gacagaacca gatacaaact tagagaagtg tctggcccgt tacggtgagc
    11161 tgcagcatcg ctttgaagaa ctaggcggtt acgtattaga agaccggatt gcagcgatct
    11221 tgcaaggctt agggattcca caccgtcagg aagcacggtt aacagaactt agcggaggcg
    11281 aacgagttaa agtcgcttta gccaaaattt tagcagccga tgccgattta cttttactcg
    11341 atgaaccgac caatcattta gatcttaaca gtattcgctg gttggaaaac tatttacaga
    11401 atacaaaaaa atcggttctg gtcgtttctc atgatcggca atttctcgat caagtcacca
    11461 caaaaacatg ggaactggaa gaaggtgcat tgattgagta tcccggaaac tacagccgtt
    11521 ttcgagtctt gaaagaagct cgcttggctg agctgacaaa aaattacgaa ctgcagcaaa
    11581 aagaagtcca gcggttgaaa gtcatgattc gtcgttttcg tcagtgggca catgaaggag
    11641 acaatgagtc cttcttcaaa aaagccaaag aactggaacg ccgcctggcg aaactgacac
    11701 tcgtcaaacc gccacctccc ccgaaaaatc ggctgcaaag cttgtctaat ggtggaaaat
    11761 ctggcaaaga agttttcatc attcaaaact tgcaccagca gtacgctgat caagtactat
    11821 tcaaagacag cagttttgca gtctatcgtg gagatcattt agccatcatc ggcgacaatg
    11881 gtgcaggaaa atcaacctta ctcaaattat tactcggtga ggaaaagcct tacggtggca
    11941 ccatcaaatt aggtagtagc ttgcaaatcg gttatttgcc gcagcagctg cagtttgccg
    12001 atcccgatag ccgtttgctg gcttacgcca tcacattgac tggaaacgaa gaagtcgctc
    12061 gacggcaatt agccaaaagt ggcttttatc aaagcgatgt gggtaaacgg atcaaagatt
    12121 tatccggtgg tgaaaaaatc cgtttggcgt tgctgaaact ctttttagaa aaaatcaacg
    12181 tcctgatttt ggatgaacca accaatcacc tcgacagcta tgctcgagaa gaaattgaag
    12241 aaatgctgca agactatcaa ggaacgctcc tagcagtcag ccatgaccgc tactttttgc
    12301 agcaacactt ccaagaagca ctcgttattg atcaggagca gatcagccgc cagccgctcg
    12361 gccaactgat tgactaggac gttttttcgc aacaaaaaac ttgattttca tacctgtgct
    12421 ttcacgattc aggcaaagta caggtggaaa atcaagtttt tattattcga tcaatgaatg
    12481 tttcggatcg gttttctagc taaacataaa gcgaagaata aagtcgacag cccacgaggc
    12541 tgatctaaaa atgttttttc tttttacata tgtaatgtcc cttataaaga tgaatgaaaa
    12601 taaaatattg ggattttcct gaaaatcaat aaataaaatg ccagtctttt cccttttgac
    12661 cacttttcca agcaaaattc cctaagaaga tcctgtaaaa aagaagggaa attgaagtta
    12721 aattaccatt ttgcggtctt ctccgcaaaa aaaacacatg caagaattcc ctcttgcatg
    12781 cgtttcatct tttatcctaa cgaatgcttt ggcttagccg cgcagacgct ccacatcacg
    12841 ggcaatcatt agttcctcat ctgttgggat cactaatact cttacttttg attcttttgt
    12901 agagatatct acttcttcac cacgcaattc attcaacgct ggatcgattt cacaaccaaa
    12961 ccaagacata cctttgatga tttcactacg tacatgtccg tcattctcgc cgataccagc
    13021 agtaaagaca attgcatcaa caccattcat cattgtcaca taaccgccga tgtattttcg
    13081 gatgcggtcc acgaaaatgt cataagctaa acgaatatgt tctttttcat agttcttttc
    13141 aagatcacgc atatcactag acaatcctgt taggcctagc agaccagatt tcttgttcaa
    13201 aacattgatc atttcatcaa tatcaatctc taatttttcc attaaatatg ggagaacagc
    13261 tggatcgata tcacctgaac gtgtacccat tgtcacccca gctaatggtg tgaatcccat
    13321 agaagtatcc atggatttac ctccatcgac tgctgtgatc gatacaccat ttcctaagtg
    13381 gcaagtgatg atttttagtt cttcgatcgg tttgttcaat aattttgccg cacgttccga
    13441 cacataacga tgacttgtgc catggaagcc atattttctt actttgaaat cttcataata
    13501 agacaccggt aagctatata aataattttg ctttggcatt gttgagtgga aagatgtatc
    13561 gaatacagcc acactcaaaa tgtcaggtaa aatcttttta aacgctttga tccccattaa
    13621 gtttgcagga ttatgcaaag gagcaaattc tgctaattca gcaatgcggt ccattacttt
    13681 ctcatcgatc actactgaat ccccataagt ttctcctccg tgaacaacac ggtgccctac
    13741 tccggtgatt tcatcataag agcttaaaat ttgcagttca atcaactgat ctaacagcat
    13801 ttttactgct acttcgtggt tttcgatatc catgatttgt tcgaattttt gattatcgcc
    13861 gtacttgatg gtaaaaatag aatcttgtaa accgatacgt tcgacgatcc ctttcgcaac
    13921 tacggtttct tctggcattt gatataattg ccatttcaaa cttgaactcc cagcattgat
    13981 tgcaattgtt ttagacatat gactctcctt ctcgttgaat gattacagag cagatgattt
    14041 ccagttttta aactcgttga aaaattccgt caccttctct ggttctttta atgaagcaag
    14101 tttcacaagc aagacttctt ttacttgttg tgcttgtggt ccttttttct gtaagatcaa
    14161 aatacttttt tgcgattgtt tattacggaa caattcatct ggcaattgaa tcattccttg
    14221 aagataacct tcttctttaa accatttttt gatttcttca ctttgatccg tttctaaaaa
    14281 tccactcggc atcaaaaaca aaccaaaacc atccggcttc acatatttca ttgattgctc
    14341 tagcaataaa tgatgtgcat aactatggcc ttcttcggta cttgttaaaa actcttttgc
    14401 tttttcgtca ttcggataat aaccgattgg caaatcgctg attgcaaaat cgacaggatc
    14461 tattaataat tcctgcaacc cgtcttgatg aaagtattga acatttgctt gtgtcaaatc
    14521 acttgtagaa gctgcgacag ccaacaatgt atcatcaata tccacaccaa ttccttctgt
    14581 ttggtacccc gcattcgata ggtttaaaag aactgttaat aacaagttgc ccattcctgc
    14641 cgcaatatcc agaatcttaa ccggtgcttt tttatttgtg tatagttgtt caataagaaa
    14701 aacaaataaa aaaccgatac tatcaggagt gagttgatgg ttcgcctgca aatgttctgt
    14761 ctggctgccc ttcaataaaa gtaattgaga cagtctgcgc cattcttctg gctcgaaaga
    14821 aagtttcttc aactcttcat aaagagctgt gatccgttgc gttgtctctt ttgtcggcac
    14881 tccatccacc acacgtactt gataatcatc tattaaattt tctgcatttt cgacataggc
    14941 ttctaaaaag gaagttccca atgcattctg taaaagctga atagcttcca ggttttgatt
    15001 aaacccttgt tccatttttt ctggtaacat ggtgcacctc atttctagat atattataac
    15061 aattttttca cctgtttaat tctatcgaac aattgacaaa atttcaatcg ttttcactgt
    15121 tttttatttg tgaaacggca aacatttttt tattaaaaac agtgttataa cagaaaagag
    15181 ggcgttttca cgaacattcc atcattctgt actagtatct gtatcatttt ttacttcttc
    15241 tgtgaaccga aagataaacg gtgagacttc cacctctaag aatagggact gttgtaccca
    15301 ctgatatgtt acagatccag ttgtgtagta aaaaactcct tccgaacttt cggcaagcgt
    15361 agactgttcc gataaagcca tctgcttcat tatttttgct atataataat ttttgattcg
    15421 tatattaaga tccattgaca aacggtagct ttctaatgct gccatcaata aaaacgtaca
    15481 taaaagaaat aaaagcaaga cagaaaataa aatagagccc ttataacatt ttctactgat
    15541 cgttttttgt ccatatggca tatccatctt ctccattttc aaagtgtaca taaaaagaaa
    15601 ttgtgtgttc tttttccaat aattgaagct cagtgatact tgttaacata ggttgatgtc
    15661 cgccggaagt tcttctttta cgtatcattg cctgatatct ttcgatgata tactgtttat
    15721 cctcgatcgt gtagtgcagt tgctgatttt tcacagagac attgtgactg ctcttgagtt
    15781 cttcttctaa ttgtgctaaa aatattagcc actctttttg atctttacgc tgcaaaaatt
    15841 cacttacttg gatggtttgc tgaatcaaaa gattgaatgt gagcaataga ctgctcaata
    15901 tcatcagtcc aattagacat tcaatcagcg taaatgctgg aattacttgt tttttcaata
    15961 ctgatcgctt ctttctccat attgacaaac actttttcga ttactccatt attatcgaca
    16021 atttggatat cgtatgggat catttcttcc cttcgctctg gtcttttgtg atacatccgc
    16081 tgcgtcttga tatcttcata aatcattcga ataaatagca gttcctcccc acttttcttt
    16141 tctgcctgaa gcaaaaacag ctggccaaag ctcactaagt agacacagac agaagcgatc
    16201 ataagcgctg taagggactc caacaaaaca aatcctgttt tattcaattc tttttgtata
    16261 gcgaccactc cctatctgga attgatacgt aatcgtccgc tctttttctt cccaataaaa
    16321 ttgataagct tttagtgaac tttcattccc ggttctagca gcaaaagtaa tagaagcatg
    16381 gcgtttcaag gtaatctcat caggaatatc taatacttcc caatttattt tatctggatt
    16441 cggaacatga aaaatgattt gattctcttc actattccaa taaaagcctg tttggatctg
    16501 attcacaata gcgatttttt gcgtagaata gattcgtttt tcaaattggc cgaaaaattg
    16561 atagactgcc aattcattct tccatgcaga aaatgcgaga actggaaaag aaaggatcgt
    16621 tgtaagtaat aaaatcagta agagtgattc aaacaacgta tatcctgatg ctaaaacctt
    16681 accttcaagg ccgctgatag atttctgcat gttctcgttt gatatagcct tcattcaaca
    16741 attcctccac agtaggaact cttccattat tttgaagcgc atagagctct ttttgtgtgt
    16801 ctaccaactg gatgatcgct tcacgacttt cttggtctac atgattccta taacgcgaca
    16861 aattagggac gaacaacaac actaatacac tgatgatcag taagacgatt agcatttcga
    16921 tcaatgtaaa accgctataa taagatattt ttctcttttt catggcaaaa cctcctctat
    16981 gcttccgtaa acaggtaata aaacagcggc ataaactaat aaaatcaaag ccgctacgcc
    17041 caaaaaaatg attggctgaa taaaatgcat ccagtgatga agacgtttga aaagctgttt
    17101 tcttgtccat tcgctgtaaa aaatcagttc tttacctaaa cttccctttg cctctccctg
    17161 taaaacaatt ttactgaatt cttctgatag aaaacgataa ttctggaact gctcagccaa
    17221 tgacatacct gcctccaatc cttgaatcaa cttagaggca agatgctgaa tcagactttc
    17281 ttgtttcgtt tcctttaagc agactatgat ttgccttaat tcgaaacctt catgaaaaag
    17341 ctttccaatc tctagtgaca aataagccga ttgatacaag gaatatatcg gtccaatcaa
    17401 taattgtctg cttatccatt cactctgatc aacaggagac aacttcatca gacgatacct
    17461 tactagtaaa gccaatagag taaccgctag gatgaatagt agaacatacc aatgaaagac
    17521 ttgtatcagc tgaatacccc aatgctcttg catgaccatt cctgaaccaa taagctgagg
    17581 caaaacgaac aaccgcatac taaacaagat acctagcaaa aaggtgagca atagcatcgg
    17641 atatgcaagc agctttttca aatcattgcg aaatccttca acaagccgaa actgtttggc
    17701 aatttccaac aaagttgtcg gcagattccc atgacgttca gctaactcga tctgtaccag
    17761 ttgctctgct ccatatccta tttgcgaaag tacctcacta aacgcttgcc cttcctttag
    17821 ctgatgttgg atatttgcca aaataacagg tggaaacgaa cggatcgtga gtaataaata
    17881 aagggattct tgcagactaa aaccagataa aagcagttct cccatggttt gtgcaaactg
    17941 gttttgctgg cttcttgtta atcctttaga atagttctga ctgccaaata ttttcctgag
    18001 cttcttcata actttcctcc catgattttt ttactgattc ttcctccata aattgataac
    18061 tccataaaac agaaggatgg tgattggcat cttccaaaag ttcttgataa atgacaccag
    18121 ctaaacattc agatagctct tcttttttct ttgtcagttc atggattctg accaatgtac
    18181 cttcaagatt gcgtgcatgt acggttgcaa aaacccggtg ccctgttagt gctgctctta
    18241 ccgcagcatg tgcagttgat tcatctctta tctcgccaat gattagcaag tctggacgat
    18301 gccgtaatgc gagtctcaaa agtgtatcat aagtctgtgc tattttcgga ttgatctgta
    18361 gctgcagaaa aaacggttct tctatctcta caggatcttc gatagtaatc acttgcaatt
    18421 tttcttccaa agccaaccga tacattagag aagtcttgcc tgatccgact ggtccgctga
    18481 aaaggtataa tccacgtctg acggttttct gtaaaatagt tgcaaaatct cgagaactaa
    18541 agcaacgatg gggtgtttca aaagtaccgt ataatagccg aatcactaaa ctctctcgtt
    18601 gaaggtaatc tcctacagaa gacaaccgca gacgaacttc ctgtttttct aaagaataag
    18661 taaatgcacc taactgagct tttctttttt ctccgatatc catctgtccc aaatacttaa
    18721 atcttgagat cagttgctgt ccatctgccg aagagacttt cttccagaat gttcgttttt
    18781 ttccatagcg aaaatagatg taaaatactt ctgaagctgg caatatatat acgtcttggg
    18841 catgtttggc aaaactttca cttatcattt gatccgataa tcgttttatt tccattattc
    18901 tcccctccac aaaaaacata cgtctttttg atctttttgt ttcaaataaa atgaatttac
    18961 aaaaaaatat tggcaaaaac cgaatttatg ttatacttat aaaaatttat cttgggaggc
    19021 actccattgg aaacaacatt aaggaaatat atcgaagtag caagtaaaaa aaagcctgct
    19081 gacctagtga tcaagcatgc taagatcgtc aacgtcttta cgaaagaaat catggaaaaa
    19141 gatgtagcaa tctgcgaagg aatgatcgta ggaatcggtg aatacgaagg aaaaagcgta
    19201 cataatgcta atgggcaata tctagtgcca ggattcatcg atggccatgt gcatatcgaa
    19261 agctcacttt tatcaccaag cgaattttca aaagtatcct tgcttcatgg agttactact
    19321 gtagtgacag atccccatga aataggaaac attgctggga gtacaggtct tgagtttatg
    19381 atagaagatg caagacaaac actcatgaat atctttttta tgctaccttc ttgtgtacct
    19441 gtaacccctt ttgagaccaa tggtgcaaat ttaaatgcaa aaaaattagc ccctttttta
    19501 caaaaacccg aagttttagg cttagctgaa gtcatgaact atcaagcagt agcaaataat
    19561 gaaacagaca tcctagaaaa gatccaattg atgcaccagc ataagaaaaa aattgacggg
    19621 catgcagctg gtatcggcat ggaagagtta aatgtttatc ctgcagcagg tattcggacg
    19681 gatcatgaag ctacaactgc aaaagaagct aaagaacgtt tggatcttgg tatgtattta
    19741 atggttcgag aagggaccgt tgcaaaagat ctggcatcgc tactaccggc gatcacacct
    19801 gaaaattcaa gaagatgctt ctttgtcacc gatgataaac tgatcaatga tttagtaaaa
    19861 gaaggatcaa tcgatcatat cgtccgtcaa gctattgcac ttggaatcga tccattacaa
    19921 gccattcaga tggcaacatt gaatgcagca gaatgttttg atttgaaaac actaggtgcg
    19981 attgcccctg ggtatcaagc tgatttcttc ttaacagacg atcttaccaa gctgccaatt
    20041 catgaagttt tctataaggg aacattagtt gttcaatctg ggcaactcca agaccattta
    20101 tttgctgatc attcaaattc atatacgtgt tgtctgccaa aactaaacgc tcaaccatta
    20161 aatccacatt cgttagagct gcttctttct tcttcacaag cccatgtgat cgaaattatc
    20221 ccgaatagtc tagtgactaa agatttggta gaaaaagtgg accgacaagg cgaacgattc
    20281 attccttcaa tcgaaaaaga ccaattaaag attgcggtga tcgagcgtca ccatcaaacg
    20341 ggaaacattg gattaggcat agtgaaagga tttcagctga aacaaggagc gatagcgaca
    20401 acgattgccc atgactcgca taatctagtt gtcgttggga caaacgacga ggatatgctg
    20461 tatgctgcta atcttttgat tcaaaaaggt ggcgggatga ccgttgtcaa caaacagcag
    20521 gaaatagctt gtgtttctct tcctataggc ggaatcatga cacaggaacc tttttcgatc
    20581 gtctatgaac agttgaatga attaataact aaagcacatc aactaggagc agctaaaacc
    20641 tttgatcctt ttttgacact ttctttctta acattatctg tcatacccga attgaagatc
    20701 accgacaaag gattattttc attttctcgc ttccaattga ttccaccttg tggaaaaacc
    20761 gatgaaataa atacatttta aatcaaataa atcccagaaa atacattaca tctgtaatgt
    20821 tcattttctg ggatttattt atggatatta atgtttttat gaaagcagaa agttagtttc
    20881 acagactttt ctttttggtc ttatcttgaa attgagcttc tactcgataa atacgttgac
    20941 cttttgaaga aaatttttct tcatactccg tcataatatt gccttcaaat tgactttgat
    21001 gtaaatctaa ccatacctgt ttgaggatca tgccatattg agaaaaactt gccagagaat
    21061 actcaaacaa tccttgatta tctgttttga aatggatttc tccatttgga cgaagaatct
    21121 gttcatctac agcaagaaaa gtcttatatg tcaatcgccg tttttcatga cgtttttttg
    21181 gccatggatc ggaaaaattc agatagattt gatctacttc gctgtcagcg aaatactctg
    21241 tcaacgctga tccatccaca tgtagaagtt tcaggttagg aagcggttgt tcgatcaact
    21301 tgtctagtgc aatcgaaaca acactgacct gcatttctac tccaatataa ttgatttcag
    21361 gatgggcttt tgccattcca gtgatgaact ggcctttccc cataccaatc tcaatgtgga
    21421 ttggatgatc gttttcaaac aattcattcc attttccctt ccacaatgtt ggatcactaa
    21481 ttacaaaatt cgggtgagcc gctaacattt ccgctgctcc cggacgattt cggacacgca
    21541 ttatttatct ccattccttt cctctaatta aaaaggtctg gccaaatctc cgcagagttt
    21601 gggccaaacc tcagccactg aaaattcgtt ttaattgtaa aatttcttcg ttcatccgtc
    21661 gatgatcttc acgtttgcaa tatctggtga tttcttggag caaattcatg gccgcatacc
    21721 aatgaaattt ttcaagcatt tcgtcagtcg ggcgtatgcc ataactgatc agccactgat
    21781 tccagctaga cagtggcaca taatttccta aaatcgttgc aatatccacc gcagggtctg
    21841 caaacatgat tgaatcccaa tcgactagat ataaataatt tttacatact aaccaattac
    21901 gatggtttac atctccgtgt actactgata agttttcctt atctattgac gggatctgct
    21961 cacgaagaaa acgatggaca atttgcagaa attgattttt ctggagttct tccggtaatt
    22021 ctttttcata tttttgcagc atcttctccg gcgtcatgac acgaccaccg atacgggtca
    22081 gcatggtttt aagagaattt gaatgatgta aatggtacaa aacatcaatc acgtcgtttc
    22141 tttgaccaat ctcattagga gtcagcaagc gaccttccag ccactcttgt gctgtcaaag
    22201 tatcgccatt ccccgttcgt tttgtccaaa caagttttgg tgtaattcct tcccttgaaa
    22261 gagcagccaa catcggagtc gtgtttcgtt taataaagac tttgtcctca tcacgcatgc
    22321 ctatgtatgt ttttcctgtg tcgcctttga tgggacgtaa ccgccactct tggtctaact
    22381 gaaactccat catgcattct cccgtcctct tactctttgt tttgcagtac ttattattct
    22441 agtcgcctga acactacacg tcaagcacat atacgttctt tttcatttaa ttgccattca
    22501 aaaaaccagc ttttctcaaa aaaaatagaa cgaacagcat tcttactgtc aacgctctat
    22561 ttttcattct catttttacc ctacacagct ttgttttcag tctgacatct tcttcaaacg
    22621 agttggtaca tatagtttaa caaagagcat agtaacaatc aaataagccg ctagcggaac
    22681 ccatcgctgc tgcccatcta atacgactgc agcaatcaaa ccaaaaataa ccgacgcgat
    22741 aattaatacc catgtgagta gatactgaat cgcctgcttc ttctgattct tatggattgg
    22801 ataaagctga acgagaacca tatagcggaa ctggttcgct aaaggtaaca gttggaaacc
    22861 gatcaaatat aaaaataaga aaccgataat cagactaaaa taaaagtcga ctactccggc
    22921 aatcaataac ccacctataa ttactaatcg gaaataaaga ccactaaaat cagaacctct
    22981 tgccaatctt cttgcaaaca agtaaagata agtgtttttt gattctcttt taatatgagt
    23041 caatatagga tccaaatagc gtcttctttt gatctttgcc gtaatttcag gaacatctgt
    23101 gaataaattg ataaattgat agatctgatg caacctatgt tcttctacat gcaccatttt
    23161 ctcccaatcc aatccttttt cttgttgctt ccacaaaaac cagtaatata gacttgcctg
    23221 tactaatgca cctgctattc ctgcccatat tagccaataa aatgatagaa acaaaaagat
    23281 cagagaagaa aaaaaccagc ctaatttcca ataacgaata acttgttgat ctagtcgaaa
    23341 caattctgct ctcttgattt gcagatgact gaatttcaaa ctccataata tgatcagata
    23401 gacgaaaaac atcgaaaagg attgctccgt actaaccacc actaatggca tggccaatcc
    23461 gcatgccaat atcaaaaaga tcaacggaaa aatgcaagaa tagcgaaaag ctcgttccaa
    23521 ataggtttgc atttcttttt ctttaggaag taaaaaaaca atatctgctg cctctgcaaa
    23581 agtagccatc ttacccacga ataaacagag catccaaaat acagagatta tgagccctcc
    23641 aattgcaaac ggtggctgca gggttttcaa ccaattagaa taatataatc ccacaccgcc
    23701 aatcaagaaa agacaaacta aaacaaaatg atcgttcaat acataacgca tatatttcat
    23761 cattttcttt tgatgtcttg ctaaacgttt ttgaaaaaaa ctactcatgt gcctgctcct
    23821 ttgttaaagc aatatagatt tcgtccaaag aagaatccgg cagctgaaat tcttttctca
    23881 acatctcaag agaaccgtct gctcgtatct ctccgttatg taagaccaca aaacgatcac
    23941 aatatttttc tgcagtagct aaaatatgcg tagacatcaa aataccggct ccttcttttt
    24001 tcatttctac catcaattca agcaatgcat tgatagctag tggatccaag cctaaaaatg
    24061 gttcgtcgat gatatataaa gaaggctcga tcaaaaaggc gcataagatc atcactttct
    24121 gtttcattcc ctttgaaaaa ttagctggga accagtcaag acgattagat aaccgaaaag
    24181 tctccaacaa cttttctgct cttttcattg ctatatccat aggtatatcg taggccatcg
    24241 cagtgacttc gatatgttct ctcaatgtca attcttcata taaagaagga gtctctggaa
    24301 tatacccaat ttgtttgcga tattcttccg tatttttctt taaggataga tgattgattt
    24361 ctatcgttcc ttttgaagct tccagcagac cgataatgtg cttgatggtc gtacttttcc
    24421 ctgctccgtt cagtccaatc aaccctacta tctctccagg ctgtacatcg aaactgatat
    24481 cttttaacac agggagatgt ccgtaccccc ctgtgacatg atcgataatt aaactcattc
    24541 tttttcctcc gccatttatt tttatttatt ataccacgaa gaaaatgacg gtattttgtt
    24601 tttttcctta caatagcttt aacttatggt aaagttaagc tgaagtattt attctttata
    24661 gaaaggatgt tttatatgga aaactgtatt ttttgcaaaa tcatcaatca agaaatccca
    24721 agctataaaa tatatgaaga cgacaaggtc tatgcttttt tagacatctc tcaagcaacg
    24781 aaaggacaca cattagttgt gcctaaacgt catgtagcag atatttttga atatgatccc
    24841 gaacttgcag gagaggtttt ttcgcgtgtg cctaagattg ctcaagcatt ggaaaaagct
    24901 tttccagaaa tgaaaggtct taatatcttg aataacaacc gtgaactagc ctatcaatct
    24961 gtttttcatt cacatatcca tttagtccct cgctacagca aagaggatga tttttctatt
    25021 cactttgtca atcatcaaga tagttatgga tcggaagaac taaaagctat tcaagaaaca
    25081 atcgtaaagc aggtgtcctg cgatgattaa aaactttttc aaaggactgt tatttggagc
    25141 tgctgctggc actgtcggag gtctgttatt ggcgcccagg agcgggaatg aaacaagaaa
    25201 gaaactgatc gaagaattgg aagaagcaac aactctgaca aatgatttaa ataacagcct
    25261 gaatcatttt aaaagcgcat tgatcgaaac aaaagaaaca gctgaatctg tgattcctgc
    25321 tttccaagaa gcaatcaaaa aagatatcga agattttaaa ttccaagccg aaccacgtat
    25381 cacacaaatc aatgaacaag tagaaaaact aaaagctgat ctgccagagt atcctgaaca
    25441 aatataataa tgattatttt ctcaacagat ttaactatat aagtgagaaa agtttgcgta
    25501 tttcctagga atgtgatgaa attttgaatt ttgtttgaaa ctaccacgac aacgttttta
    25561 aaggaaatgg gatatggtat agttaatata taaaaataat aactaattac aggagtgcaa
    25621 attttaatga aaaaaaaatc aattattttg gcagctacta gtgcgttggc tgttttaact
    25681 ctagcagctt gttcaggcga tactaataaa gatatcgcaa cgatgaaagg cggaacgatc
    25741 actgtatctg atttctatga tgaagcaaaa cttgaatcat cgaaccaatc cttagtacaa
    25801 cggatgatca tttataaagt attcaataac aaatatggcg ataaagtaac cgataagcaa
    25861 gtggatgcag aatacgacaa acaagcgaaa tcattaggtg atacttttga atcacaatta
    25921 gaagctgctg gatatacgaa agatacttat aaagaatata ttcgtaacaa tttagctttt
    25981 gaagctggct tgaaagctca cgtagatatt acagatgacg atttgaaaac tgcttggaaa
    26041 tctttccatc cagaagtaga agcacaaatc atcaaattat caagtgaaga tgaagcaaaa
    26101 gatgtgaaaa aatcagcgga tgatggagat gatttctcta aattagccaa agacaaatca
    26161 actgatacag agacaaaaga agatggcggt aaagttaaat tcgactcaac gacaacaact
    26221 attcctgcag aagtaaaaga agcagctttc aaattgaaag acggtgagat ctccgatgtt
    26281 attactacaa caaatcctac ttcttatgcg acagaatact acgttgtcaa aatggtgaaa
    26341 aaccaaaata aaggcaatga catggataaa tacaaagatc aactaaaaga tatcgctact
    26401 gaaactaaac taagtgacaa tgcattcaca actaaagtaa tcggtgaaga attaaaagat
    26461 gcaaatgtaa aaatcaaaga tgatgccttt gaaaatgtgt tgagtgcatt cacaacaact
    26521 tcatcaagta cgaaagactc aagtgaaact acagcttcaa caaagagttc tgacacaaaa
    26581 tcaactgatt caacaaaaga atcttcaact gaagaaacaa ctgattcatc aaaataaaag
    26641 aaaacaaaaa aagcgaagac ttatgtctcg ctttttttgt tttgaatgaa aaaaataaat
    26701 ttttgctata aatcttttgg tacatagaaa cttcggttgt ccataccgaa aatccgatcg
    26761 gtgtattttc ctggttctgt ttgacgaatc gaagtcaaca tcatctgcat cgaagcatcg
    26821 atattgtcga tttgatgtaa tatttctgct tccattattc ttggacgtac aggtgagcca
    26881 tattctaata agccatggtg tgctaaaacc atatgacgta atacgagaac atcttcgtca
    26941 gcttcgctga ttttcaacgc aagacaggct ttagttattt cttcgtcaat caataccaga
    27001 tgaccgatca aatttcctgc aagcgtatat tctgtcgaca taggaccgga aagttcaatc
    27061 acctttccta aatcatgcaa aatgatacca gcatacaaca atgctcgatt cagctcagga
    27121 tattcatcgg cgattgattt ccctaagtga agcattgtca aagtatgata agctaatcca
    27181 ctggcaaatg catgatggtt tctttttgct gccggaaact caaaaaattg tttttgatat
    27241 ttattaagta gatagcgaac aatacgattc caatgcgcat tggtgatctc aaacaacaat
    27301 tgattgattt cttcttccat ttcttctttc ttcaaagggg ctctttccat gtataaacta
    27361 ggctctcctg gctcatctac ccttgttaag cgcatatgaa gaatctttac ttgagggttt
    27421 ccttgataaa cctctctttt accgtttaga aaaacgactg tgccggctgt aaatttattg
    27481 atttcctcct ccgaagcatc ccagtattta ccatcgatag tacctgaagt atcttggaaa
    27541 gtaaaagcaa taaatttttt tccgttttta gcgattctta catctgcact tttgatcagg
    27601 acaaattgtt caaactgctc atcgattact aaatcacgaa tttttttcat tatttgctcc
    27661 ttctaattgt tttacgattt ggttcatctc atggtaatag cttaccattt ctttatcaga
    27721 agaaaaacaa attatttgat acttttctcc aaatttagct aaaagttcag ctaattgata
    27781 ctttcgctga tgatcataat gaagccagcc atcatcaatg atgatcgggc atatcgaacg
    27841 gttagcttct aatgagaggt aagcaaatct catcgccata atcagctgat ctttcgtacc
    27901 tgtagataac tcatagatgg gaaattctgt atatccgtct gttaaagtca gtgtatcatt
    27961 tttcagtaat atcttctgat aatttccttt tgttaaaata cctaaataat cacctgctgc
    28021 tcttaacaac tggggtaatt gtctttcact catctctgtt gaaagatcac ctaagaatgt
    28081 cccggcgatt tcaagtgctc cccattcctc cagtaattcc tgtaattttg ctttttgccg
    28141 gctagcttgt tgataaagtt cgtccaatgt gccgtctctt tgcaactctt ctacttgaag
    28201 ttggagtcgt tgccgatttt cacttgcttc tttttcttct tgctggagtt gggattgctt
    28261 ttctttcagt tggcgcagtc ttacagataa tttctctttt gtcagctctt caggaaatag
    28321 tggagtaagc atttccgata attcattgta tcttttcatc tgctgttcgg cttgtttggc
    28381 tttttgtaaa aagacaggta cttccgaagg ataagataag cctgcttgag tcaattgttc
    28441 gctgtattct tgtatccata ctttttgttt ctttttcaat tgattgattt cttgcttcaa
    28501 taaagtattt tgctgatagc ttctggcaaa tttcatctgt tccatctctt gtataaaacg
    28561 ggaaagcaat accatttttt ctgaaattgt cttatcttgt atcggtaacc attctttaac
    28621 aaactcaaat ttttggtcga tttgtcgtag tttttcttgt atttcctgtt ctcttttatg
    28681 tttccgctca aatacgccat ataatttctg ataatctatg acttcctgtc catattcttt
    28741 catgagtttg agcgtatcca tctttcctaa gtgccgatga tccgcaaaac gatatacttc
    28801 ttgctgaaga atctgttttt cttctccagt tctcttttgt ttttcttctg cttcagctaa
    28861 ttgagctaag gctagatcaa gttgtcctag tttttcttgc cacattcctt ttatctcaga
    28921 gaaatcattt ttagttttaa tttttggttg ccttttgtag aagaaatagc caatcatgcc
    28981 tgcaccaatt agtaaaagga tcactttcaa gggcattggt acaaatatac ctgcaatgat
    29041 tattgctccg ccagccatca gcatatacgg tttagatgaa gagactggtg ctatctctga
    29101 ctgattttgt aataatgctg gataggtctc ttccaagtca ttcacttctg cttcaatcat
    29161 ctcttgctgt tctttagcta tttggagacg cacagaaatc tggctttcta gatcagcaat
    29221 ccttctttct tcagccagaa gatgcttcca ttcttcttca tataaaaatt cttccggtaa
    29281 cggatttttc tgccaatgcc acctttgttc caaaatcttc aattctgaat ttgtttctct
    29341 agtctcaatt tgtaatcttt gccattcttc gtttaattgt tccgctgtaa attgaagttg
    29401 ctggagatct tttagttcct gctcctgctc taaataaaaa taatatctgg ctgactgctg
    29461 atccatcccg ctatgctttt ccagtgttgc ttctaattct tccaaacgct catttgtttg
    29521 ctgataccgt ttggagaaag actccagatc taaaagaagt tgctcatcag gaagacctgt
    29581 agtgctgttt tttttgagag attgccattc ttcatataaa gaaaaattaa gttcttgctg
    29641 tctggctaat gaaaattgtt tgttcgcctc ttttaattgt gccaataact gctgctgttg
    29701 cgactcgtat tcctgttctt gttggatcaa cttgccaaaa taagcttcct gttcttgttt
    29761 ttgatgaatt ttttcttgca gctctttcca ttcatttaat ttttgattga ttagtaattt
    29821 ttggcctttt ttcttgaata attgttgtgc tttctgataa tattcttgcc gcttttggaa
    29881 tacttgagaa ctgcccgtga tgcctaaaga aatcagcgca tcatgtagct ctttttctct
    29941 caaatgatct agttccatca actgctcttg ctggaaagta aatacctgct ggaataactc
    30001 tttcgtcaaa ggtgcgatca gtttttctag taactcatca cttcccactt gatcccccag
    30061 ccacaccttc gattttccgc gattttgctg cttgtagcgc tcgacagcaa attcaccata
    30121 tacaggatgt ttaagccata ttttgccgcc ataggctgag ccgtcttttg gtgtataatc
    30181 ctttttttta cggccttttg aagggaaacc aaataatatt gcttgaataa attgataaat
    30241 cgttgatttt cctgcttcgt ttcccccata gatcagctga ttgcctgaat aaaagtcaaa
    30301 acttttttga cgccattttc caaaagcagc aatctcgatc cttaaaatct tcattcctga
    30361 tcctcctcaa ataaaaaatc ttgattaagc agctgcttgg ctttttcaag tgtttcttct
    30421 tgatattccg gcagttcgct caatattcta gccgcttctt gatgactata tacttcattt
    30481 aaaatttgtt ggaaatcttg aggtgtctga tagtattgaa ataactgttc catcaattta
    30541 gcagaagcag caagtggggt cttcgtataa aaagtattct ctatgagaga aatacgccaa
    30601 ataaagaaat catttatcct atctgagaat tcctctaaca aatagtctaa tagctctcct
    30661 gattgtatcc gttccaaaac atctgtattg agatggtcat aatcttttaa ttttatctca
    30721 attaaactaa atccatcctc tacttttgaa agctgctgac gaatgtgaac aatgacctct
    30781 tttgtcgttc ttgctttcct caatgatatt tcctttgtct tccaatagac ctctgctact
    30841 ttaatcggat gtatttggca acttccttta cttaattcaa ctagtagaat agatgtagaa
    30901 gcttgttcct ttttagtatg accttgaggc gcacctggat aaacaattgt ttgcttttca
    30961 tttaagaccg ttggcacatg gatatgccct aaagcccaat aatcgtatcc cttttcttgc
    31021 atctttgagg gttggaaagg agcatagttt cctttttgag agacgccagg ttccccgtgg
    31081 tacaacccaa tatggtaatc tgtgagttct ctagaaggaa attccatcac tttgtctttt
    31141 tgaatccacg gttgacgata actaaatcca ctgagagaga cagtttctcc actttttatc
    31201 gttatttttt ttgtctctac cgtttcggat gtaaataaat ggacattttt aggaaaggag
    31261 aaccaatacc gctctttttg atagaaatca tgatttccga atattataaa tacaggtata
    31321 tggttctttt ctaaccgttt catctgttct gaaaaatgtt tctgtatttt taatgaagga
    31381 cgattttgat gaaaattatc tcctgcaagt aaaacaaaat caacgttatt tattattgcc
    31441 tgttcaatga tatttgataa aacggtcaga ttcgtcttca atagtttttc ctgtacagtt
    31501 ttatcaagag ttgtcagtcc ttcaaatgac cgatccatat gtaaatctgc cgcatggata
    31561 aatttcacca ttaaaaattt cctccttttt ttcttttatc ataccatttt caatgttttt
    31621 tttcatcaat aaccgaaaat ttgttcgtat tatgcaaaga aattacgata aatggccgac
    31681 ttcaaaaaat aagtttattc gaaaaaaaga ttgtgacatg atttttgtca caatcctaaa
    31741 tatctatgtt atattcgctt cgtttgacac tgaagaagta ctatttgtac tggaatttat
    31801 gctccatttt tccgtattta gaattctatg ttctatacta ccttaattag tttatatggt
    31861 aagaattctt tcaaatgctt gagatactcc gtctttatca tttgaaacag tgacataatc
    31921 agcttttttc atgatttctt gatgactatt tcccattgca aaactaatat ctgcactttc
    31981 aaacatagat aagtcattta aactatctcc gatcgcgatt ctcaaaggtt tctttaaatt
    32041 gtagattgat tccaaacgat caaacaccac acctttagat acattttgag gtacgatttc
    32101 aatatttaca ggtgccgatt cagtaacaga cacatttagc atctttttca aatggttaaa
    32161 tatattagct ttcaattgac gattaggaat aaaaataaaa aatttgtaaa tgctgatagc
    32221 atccttttct ataatttcct caatattatt ataagggatt gaacaaatag atttttcata
    32281 ctcaagctca tagtttagct ctttgataga aaattcactc cctacctcgt gaaaaatttt
    32341 tttcatttta tttttataat tctgagattc ataacttccg agatttgtat atattttggt
    32401 tggaaatttc gataggacag aatcaatttt ttttacatca tttttatcga aggaagactc
    32461 aaatatcttt ttttctccat caaatccaac agaaccattt aaccctactg ctggtatcct
    32521 taaaccagat tttttgagaa tattattaat gtcttctact tctcttccag aacaaatgaa
    32581 tggaaaacag tcttccggta aatgtttcat tgttcttgca tttttttgac taatgctctg
    32641 gtcagaattc aaaagagtac cgtctaaatc tataccaatt agtttcattt catccatgtc
    32701 tcctatacgt tgattataaa ttcactgctt attgtaagtc taaatttctc acaaaacata
    32761 tactaattat acctaattta agtaagtaca aacatcattt taagaatagg aaaggacatt
    32821 tatatactat tctctgtatt tttgatttat aaataaatat gaattgaatc caaatcagct
    32881 atattactat tttataataa ccaactataa aaaacgaagt taggtgaaaa actaacttcg
    32941 atattcataa aagactctat tagttcaact tagcaaaaga tttaaacaaa aaaagatgaa
    33001 ctatttctag ttcatc
//
LOCUS       DAFEOM010000024        29154 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.025, whole genome shotgun sequence.
ACCESSION   DAFEOM010000024
VERSION     DAFEOM010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.025"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(<1..1030)
                     /locus_tag="LP524_001301"
     CDS             complement(<1..1030)
                     /locus_tag="LP524_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL2234365.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="LP524_001302"
     CDS             complement(1112..1801)
                     /locus_tag="LP524_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBL2234366.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="LP524_001303"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="LP524_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBL2234367.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /locus_tag="LP524_001304"
     CDS             complement(2328..2831)
                     /locus_tag="LP524_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBL2234368.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3035..3162)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="LP524_001305"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="LP524_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBL2234369.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="LP524_001306"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="LP524_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBL2234370.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="LP524_001307"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="LP524_001307"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HBL2234371.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="LP524_001308"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="LP524_001308"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HBL2234372.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7462)
                     /locus_tag="LP524_001309"
     CDS             complement(6443..7462)
                     /locus_tag="LP524_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBL2234373.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7635..8540)
                     /locus_tag="LP524_001310"
     CDS             complement(7635..8540)
                     /locus_tag="LP524_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBL2234374.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="LP524_001311"
     CDS             complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="LP524_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBL2234375.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9245..9415)
                     /gene="secE"
                     /locus_tag="LP524_001312"
     CDS             complement(9245..9415)
                     /gene="secE"
                     /locus_tag="LP524_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBL2234376.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="LP524_001313"
     CDS             complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="LP524_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBL2234377.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9753..10397
                     /locus_tag="LP524_001314"
     CDS             9753..10397
                     /locus_tag="LP524_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBL2234378.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10453..10956)
                     /locus_tag="LP524_001315"
     CDS             complement(10453..10956)
                     /locus_tag="LP524_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBL2234379.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10969..11015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11553..12518
                     /locus_tag="LP524_001316"
     CDS             11553..12518
                     /locus_tag="LP524_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBL2234380.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12617..13789)
                     /locus_tag="LP524_001317"
     CDS             complement(12617..13789)
                     /locus_tag="LP524_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2234381.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13776..14921)
                     /locus_tag="LP524_001318"
     CDS             complement(13776..14921)
                     /locus_tag="LP524_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2234382.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14935..15867)
                     /locus_tag="LP524_001319"
     CDS             complement(14935..15867)
                     /locus_tag="LP524_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2234383.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15984..17063
                     /locus_tag="LP524_001320"
     CDS             15984..17063
                     /locus_tag="LP524_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBL2234384.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17060..17689
                     /locus_tag="LP524_001321"
     CDS             17060..17689
                     /locus_tag="LP524_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2234385.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17799..18026)
                     /locus_tag="LP524_001322"
     CDS             complement(17799..18026)
                     /locus_tag="LP524_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL2234386.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18129..20717)
                     /locus_tag="LP524_001323"
     CDS             complement(18129..20717)
                     /locus_tag="LP524_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBL2234387.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20737..21396)
                     /locus_tag="LP524_001324"
     CDS             complement(20737..21396)
                     /locus_tag="LP524_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBL2234388.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="LP524_001325"
     CDS             complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="LP524_001325"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBL2234389.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22169..23035)
                     /locus_tag="LP524_001326"
     CDS             complement(22169..23035)
                     /locus_tag="LP524_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBL2234390.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23144..23785
                     /locus_tag="LP524_001327"
     CDS             23144..23785
                     /locus_tag="LP524_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBL2234391.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="LP524_001328"
     CDS             complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="LP524_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBL2234392.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25226..26125)
                     /locus_tag="LP524_001329"
     CDS             complement(25226..26125)
                     /locus_tag="LP524_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBL2234393.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26127..26924)
                     /locus_tag="LP524_001330"
     CDS             complement(26127..26924)
                     /locus_tag="LP524_001330"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HBL2234394.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26902..27606)
                     /locus_tag="LP524_001331"
     CDS             complement(26902..27606)
                     /locus_tag="LP524_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBL2234395.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27737..28312
                     /locus_tag="LP524_001332"
     CDS             27737..28312
                     /locus_tag="LP524_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HBL2234396.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28430..29107
                     /locus_tag="LP524_001333"
     CDS             28430..29107
                     /locus_tag="LP524_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBL2234397.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8582 a         6173 c         5242 g         9157 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
     8341 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
     8401 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
     8461 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
     8521 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
     8581 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
     8641 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
     8701 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
     8761 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
     8821 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
     8881 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
     8941 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
     9001 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
     9061 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
     9121 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
     9181 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
     9241 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
     9301 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
     9361 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
     9421 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
     9481 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
     9541 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
     9601 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
     9661 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
     9721 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
     9781 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
     9841 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
     9901 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
     9961 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    10021 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    10081 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    10141 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    10201 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    10261 acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg
    10321 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    10381 aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt
    10441 cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa
    10501 tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca
    10561 aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa
    10621 gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc
    10681 tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa
    10741 accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt
    10801 aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac
    10861 aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat
    10921 tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt
    10981 tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag
    11041 acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg
    11101 taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat
    11161 tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa
    11221 ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct
    11281 gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg
    11341 cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc
    11401 tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc
    11461 attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg agtattaaaa
    11521 tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat atattgatca
    11581 atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta ccggtattaa
    11641 aaaataagca tattgactat acacttcatt atagtgaata caaaggacac gatgctgaga
    11701 ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa cagaaaacaa
    11761 tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat caggtacttg
    11821 atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca ggttctggaa
    11881 atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt cagctgattt
    11941 tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa ataagtgaaa
    12001 aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc gtccatgcga
    12061 caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca ttttcgtatt
    12121 tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg gtagatgcag
    12181 gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag catccttttt
    12241 ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc attgattttg
    12301 tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta cttatacaga
    12361 aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta cgtatcgttt
    12421 caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga ccttttgata
    12481 tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt tgtcagaagc
    12541 tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt gtcacagtcc
    12601 tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag tttctttctt
    12661 tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag acacaaccat
    12721 tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg atcaagttat
    12781 tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc atttctggca
    12841 agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata aaaatatctt
    12901 tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc gaaaagctaa
    12961 tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt atatcccacg
    13021 aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac agcagcgagt
    13081 aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg ggcgcactct
    13141 ggtttcgttt actgatctct ttgcgattaa acgctagatt gatgacgctg aaaccgctaa
    13201 aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc aacagctttt
    13261 tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag agtgccattt
    13321 gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga atcagtgttt
    13381 gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct gggcgactct
    13441 gtatcttgat cgtcgccatc tttcctactt cgacttgttc ggtaaagtct gcaggcagat
    13501 gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt tgtgaggttc
    13561 catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca tcgactacag
    13621 gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta ttttcaacat
    13681 caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct atcagtaaat
    13741 tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata acgcacccct
    13801 ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa ccatagaaga
    13861 ttggtataaa aactagccaa atcagtgtag aaataaagtt tatacaaact ttcgctgatc
    13921 aagttgacga gatttaattg accaacaatc ggaatatgaa gatcaatcca atacttcact
    13981 tgttccgacc ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg
    14041 ctatcctttt gcaccagatt catttttggt atcaaattac caaccaatgt acctaacaaa
    14101 atcgcacaca acgaccccag agcggtaaca atcatcaacc attgccaacg ttcaccaaag
    14161 tctacttgat agataaaacg aaagacagct aacataatat aaatctctgt caaaacaatc
    14221 gtaaagcttg ctgccaaatt agcgattgaa actaacgatt gctttctagg aatcaaggac
    14281 aagcgaatac catttgacga ttgatttgct tgctgatcct gtacattgcg gaccccccac
    14341 atcgtcccgt agagaatcgc cattccaatc agagtgaaga aataaaaact tttgaaactc
    14401 atattacgac ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg
    14461 ggcgcgagat caacgttctc cacttctgcc actttactcg aaattatacc aatacgctgt
    14521 aaaaattgat ccatgacgtt tttcatcaac gtctgactca ttcctgcttg acccacatgg
    14581 agcgaaacag atgaaccatc aatgagataa aaaccagcca aatcatctgc atttagcttt
    14641 tcattcgctt ctacttccga tagttcctgt acttcaaata atggtttatc attgacttcg
    14701 acttgttcta agactgtggt aaaattttcc gaaagcgctg tttgatccgc tttgattaca
    14761 attccagtat taatcgtttc aagattgcca ccttgatcaa tctcgccaaa ggccgccata
    14821 aacatcaatc ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga tttatttcgc
    14881 agcagtattt ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc tcctctagtc
    14941 tctcaaatcc ttgccagtaa tctctaaaaa gacatcattc aaggacgctt ctttggttgc
    15001 tatttttccg taactgatct tttcttcttg taagattgta agaatcggca acaatactgt
    15061 atttagttca gtagttgtta cttggagctg acctttttta taagcgattc catcagaagc
    15121 tattgttcgc tgaatcgctg acaacacatt ttctgggagt aatgggatgt cgatcgtcaa
    15181 acgatccgcc gtccgtacca tatcttttag agattctttg gttcctttag caatcacctg
    15241 tccttgatcc atgatcacga tctggtcgca aagaatttct acttcttcca tgtaatgtgt
    15301 tgtatagaca atggttgctc cttgtcgatt cagctccttg atactttcta aaattttatt
    15361 ccgactttgg gggtctacag ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg
    15421 ggcaatccca catgcgatgt tcaaccgacg tttcaagcca ccactaagtt ctcctggacg
    15481 aaacgaaaca tatttttcta atcccactaa ttgaatcacc tcttcaatca aggctttcct
    15541 tttctctttt tcggaaacat ataaaccgca aaaataatcg atattttctt gcacacttag
    15601 ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc attgccttga cagcataatt
    15661 ttcaggagtc attgttgtcc cgaacaattt aatggttcct ttgtcataat ttaacaaaga
    15721 aagcatacag ttgatggcag tcgatttccc actgccgttt ggacctaaca gacctaagac
    15781 ttctccttct tgtacttgca gatcaaaatg attcaatgct acaaaatctc catagcgttt
    15841 gactaattta tcaatttcga taatcatcat gaaaaactcc ttccaaattt cttgttgatc
    15901 tgactataat aaagtgcaag aaaagaaact agtgacgatt ggctaaacca cacatgacat
    15961 ttgtcacaat gaaggagtct gctatgcata aatctcggct actcaacgaa actagctttc
    16021 tactggttat ctcattgctt ttgctggtaa gtcaaggatt cgttgctatg caagttatct
    16081 atttattgat cgccgtttta ttcactggtg tcagttattt actgcctaca aagccaaatt
    16141 ggattttcca agttattcta tttcttttat gtgccttttt acctgatctt gtcctgtttc
    16201 tgccagcgac gatgcgagtg atcgcccctg aagaaagcca ccttgattac ttgtttttgt
    16261 taggtttttt gttgatttcg acggttggta atcatccccc catcaaggtc gcctttgtcg
    16321 taattttgct gtttttttcc ttttacttac gaaaaaaaga tcaacgaacg accgatcttg
    16381 agaaaaagta cattgtctcc aaagatactc attgggaaaa acaacaagag ttatcggaac
    16441 aaaataaagc attggccctg tcacaagaaa cacaattagc cttgcaaatt gtggaagaac
    16501 ggaaccgaat tgcccgagac atccatgaca atgtcggtca tctgctgtca agtgcgattt
    16561 tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa actaaaagaa ccattagaaa
    16621 atttaagtgc caccgttcac actgggatgg atcggattcg tcaaagtgtc catgacctcc
    16681 atgagacatc gatctctttt caacactcct tagagtttct gatcgctgat tttccttctt
    16741 ctgttacgat tgaaggaaac ttgtttgaag gattgaatga aacgcagcaa aactgcttct
    16801 taatggtcat caaagaagcc ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg
    16861 ttttacattt tcgaacgttg ccggcctttt atcgcttgca aatcacgaat gatggcacca
    16921 gcccacaaaa aaattctgct cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag
    16981 aacaaaaggg acaagtccat attttccaag aaaaccagca atttcagtta acgatcattt
    17041 taccaaggga gggacaccaa tgataacagt agtcattatt gatgatgatc catttgtgac
    17101 gaaatcgtta caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca
    17161 ttgtgccaag gatgcgctag tcttatacga aacccatcgt cccgatgttt tgctgacgga
    17221 catccgcatg cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc
    17281 caaagccatc cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt
    17341 ttccatcggt gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt
    17401 caaatccgct tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca
    17461 aatgatgaag aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga
    17521 gcttgcaatc atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt
    17581 gtacttgagt gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt
    17641 acgggatcgc acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa
    17701 cacaaaaaaa cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt
    17761 caattgtccc gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca
    17821 ggattcgttg gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt
    17881 tctagttttg attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat
    17941 tctttttcca tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct
    18001 aatttatctt cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca
    18061 tatcacactg aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat
    18121 ctttaaaatc aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca
    18181 atcatcggat ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa
    18241 acagcaggtt ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt
    18301 tccactgttt ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt
    18361 agcttttctt ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt
    18421 ccgaccaaag aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca
    18481 ttatccatga tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa
    18541 gtgacatagg cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc
    18601 gtgtagagca aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc
    18661 atcttgaatt cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta
    18721 ttccattcgt ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc
    18781 actttatctt cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg
    18841 aatacattca attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg
    18901 gtgtcgttcc aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc
    18961 attttattta acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata
    19021 tcttgtgcac ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca
    19081 atttgataag catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt
    19141 ggcagacgtc cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc
    19201 cgataaatct gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt
    19261 acttgaccag gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata
    19321 ttcgtcggaa tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca
    19381 tcaacaatca aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt
    19441 ccattcagcc ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt
    19501 cgtttagctg ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat
    19561 tttgaaggat ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc
    19621 acagtagttt tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct
    19681 tcagcgatag cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc
    19741 cgaatattct tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg
    19801 cgctggatcg aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta
    19861 gtctcttctt gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga
    19921 tcaggctgga tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat
    19981 gtctccgccg gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag
    20041 atagctgctc gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta
    20101 tctgcacgtt tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg
    20161 atgatatcca aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg
    20221 aacccgtaac gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc
    20281 tcagcaagca tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga
    20341 tcagcaatga ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt
    20401 ttcttcccga ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt
    20461 gttggctttt cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc
    20521 actaattctc cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg
    20581 actacaatct ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact
    20641 ttgtaaaaat tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc
    20701 atttcttctg gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt
    20761 ttcagttcct aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca
    20821 aggctcttca gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa
    20881 ctcacttagc tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc
    20941 tacgaataaa agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat
    21001 acgtgtgatt gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa
    21061 atgattacgc aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg
    21121 accttctaaa gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg
    21181 acgagcaagc tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga
    21241 ataaattgct ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta
    21301 actttgtggc aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca
    21361 ttgtgtcttc ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt
    21421 ttattttact ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa
    21481 ttctaaatta gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt
    21541 cgataaattc aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc
    21601 gttttcacgc ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg
    21661 ctcttctcca tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa
    21721 atgtagttta cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat
    21781 atttacatca ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa
    21841 acctagtggc tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt
    21901 tccagtgttt gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc
    21961 tctcatattt atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt
    22021 atcaactcgg tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt
    22081 tatcagaaca ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc
    22141 ctcaactttt ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag
    22201 ttccttttaa taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg
    22261 ctttgattcg ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat
    22321 tttcctctgg tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt
    22381 cagcaaactt ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat
    22441 aaaaagcctg acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc
    22501 catcttctga gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa
    22561 ccgcgttgaa tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg
    22621 cttcggcaga ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag
    22681 cattttgaat ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa
    22741 catctaatag cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat
    22801 acatgccaga ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt
    22861 taaccgcatg ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat
    22921 ttttgcatat caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga
    22981 acagctgatg attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc
    23041 cccctcattt ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg
    23101 atacaatgcc tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg
    23161 cgctgaaaac tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt
    23221 gtgtgacaac ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt
    23281 aagttatgct ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga
    23341 cttcgtctat aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa
    23401 attagggacg gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga
    23461 ggccttagaa ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt
    23521 tgacagtatt cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt
    23581 tgaccgtatc ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg
    23641 gcttaaagaa ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt
    23701 cactgcagag aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac
    23761 caaaatcgtg gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa
    23821 tgcttattgc aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata
    23881 ttgaaataaa tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct
    23941 actgctggat caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc
    24001 gccactgtga ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt
    24061 ttccaaaaac ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg
    24121 gtcaagactt cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca
    24181 agtcgacgaa cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc
    24241 agcgaaatga agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt
    24301 aaagaagcgg tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac
    24361 gctgccttta ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa
    24421 tcgctagcag cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac
    24481 aaatgatcat cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt
    24541 gccacatctt cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca
    24601 gaaatcattg cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc
    24661 tgaccttctt gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc
    24721 ttcagcacgt acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt
    24781 gtcatgatcg cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca
    24841 tcgtctgccg acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc
    24901 aagtcaacag cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc
    24961 atttcatcta ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc
    25021 gctaattctt taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat
    25081 tggtagaatt gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga
    25141 atattatttt cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt
    25201 tcatccaaac caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt
    25261 ctgcggctaa aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca
    25321 gatcataaca atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat
    25381 catcaccgag cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc
    25441 ctgtatgcag ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact
    25501 cagtctgtgc cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc
    25561 gggcgatcgg caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta
    25621 aggcctgata ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg
    25681 caaatccatg ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca
    25741 catggattac ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca
    25801 ttgtcccatt tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta
    25861 ccagaacgtg ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt
    25921 catgttctcc ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca
    25981 agacattttc tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta
    26041 acagaccctt tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt
    26101 tctcataatc ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga
    26161 tgccaaaaat gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa
    26221 taaattgagc ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct
    26281 tctgtatttt ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc
    26341 aaagtacgaa acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata
    26401 ctaggatgga tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta
    26461 gaaatcgtca atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg
    26521 gctaccatcg tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg
    26581 tccggtgttt catccaaata actgatccga acatccagca agggataact aacactttgt
    26641 tcccggttcg ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg
    26701 taaaacccta aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg
    26761 tagtgatgga acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc
    26821 tcatgatcat ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact
    26881 tctatagtct ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct
    26941 ttcgctttct ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg
    27001 ggagaataac cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa
    27061 ctgataggca gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc
    27121 ttgatattta taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg
    27181 aatctggatt tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat
    27241 caccagatga taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg
    27301 gagaactttc atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac
    27361 aaactgacac atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat
    27421 tggaatatgc cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt
    27481 gacaaattca atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag
    27541 tttcactttc aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg
    27601 ttccatagct gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa
    27661 gttttttcgt actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca
    27721 aaaaggagtc gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa
    27781 gcaagagtac gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca
    27841 gactaatatt tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg
    27901 gatccgtttc gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg
    27961 tctcgaaaca acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg
    28021 tctgcctcgt tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc
    28081 attaatccaa accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact
    28141 tttagctatt gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt
    28201 aggggatgca tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc
    28261 ttatcgtcct gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt
    28321 cattttcaca aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg
    28381 aaagaatcta gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat
    28441 ctatttattt gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca
    28501 atttatcgaa gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc
    28561 tttggtcaat ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa
    28621 tcttgaggaa cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc
    28681 ggcgcagttt caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt
    28741 cggctgtaat gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg
    28801 cggcgatttg aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt
    28861 ttctgatcaa aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta
    28921 caattatgcc tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct
    28981 ttggagaatc acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga
    29041 acatttacat acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc
    29101 taattaaaaa aggctctttg tcaaatgatg ttggtaaaga gaaatcttag aata
//
LOCUS       DAFEOM010000025        29141 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.026, whole genome shotgun sequence.
ACCESSION   DAFEOM010000025
VERSION     DAFEOM010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.026"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(<1..680)
                     /locus_tag="LP524_001334"
     CDS             complement(<1..680)
                     /locus_tag="LP524_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL2234398.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELR"
     gene            complement(746..2035)
                     /locus_tag="LP524_001335"
     CDS             complement(746..2035)
                     /locus_tag="LP524_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HBL2234399.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVTIYKSVI
                     IPNIDETSSSEIKNKLSLLKIKEKDFILDGAINIPSYLNNREANNIKKKMLIPLESEI
                     TGLLNKLTISNVDSTREKIIRTFDKINKLFKNDEAEFKKELLELKLQELELNYSYIVK
                     VNDEKEQQKAIKEQMIEEEKVRREIEREKKRIDKEERQFNSEITKLMAYMQKASADVE
                     KELYADKIKELEEKLRELETVKEDVLQRELNTRAGYVYVISNIGSFGEDVYKIGMTRR
                     LEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRKNEVNKVNQRKEFFKV
                     SLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
     gene            complement(2122..2826)
                     /locus_tag="LP524_001336"
     CDS             complement(2122..2826)
                     /locus_tag="LP524_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HBL2234400.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            3026..3292
                     /locus_tag="LP524_001337"
     CDS             3026..3292
                     /locus_tag="LP524_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2234401.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            3305..3463
                     /locus_tag="LP524_001338"
     CDS             3305..3463
                     /locus_tag="LP524_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234402.1"
                     /translation="MEEKNIKVPTLEEYTKIVVETDEQNPKTIAVITANDIESCEGFR
                     VRMTPRYD"
     gene            complement(3460..3690)
                     /locus_tag="LP524_001339"
     CDS             complement(3460..3690)
                     /locus_tag="LP524_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBL2234403.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            3762..3917
                     /locus_tag="LP524_001340"
     CDS             3762..3917
                     /locus_tag="LP524_001340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234404.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            4074..4229
                     /locus_tag="LP524_001341"
     CDS             4074..4229
                     /locus_tag="LP524_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234405.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGEKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(4226..4450)
                     /locus_tag="LP524_001342"
     CDS             complement(4226..4450)
                     /locus_tag="LP524_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBL2234406.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            4717..5052
                     /locus_tag="LP524_001343"
     CDS             4717..5052
                     /locus_tag="LP524_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234407.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            5285..6226
                     /locus_tag="LP524_001344"
     CDS             5285..6226
                     /locus_tag="LP524_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HBL2234408.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            6228..7118
                     /locus_tag="LP524_001345"
     CDS             6228..7118
                     /locus_tag="LP524_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HBL2234409.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            7161..7961
                     /locus_tag="LP524_001346"
     CDS             7161..7961
                     /locus_tag="LP524_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBL2234410.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            7976..8827
                     /locus_tag="LP524_001347"
     CDS             7976..8827
                     /locus_tag="LP524_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL2234411.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            8824..9141
                     /locus_tag="LP524_001348"
     CDS             8824..9141
                     /locus_tag="LP524_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234412.1"
                     /translation="MNLTKQEKRICRQIMLQIIGDRDMDELSQQESRMVRKISKLIGA
                     DTMDRSKPLPKLNFESFTFEKYSHLREVGYSVNAIRKALGVGSSVWAKWRKENVNEVN
                     QND"
     gene            9134..9316
                     /locus_tag="LP524_001349"
     CDS             9134..9316
                     /locus_tag="LP524_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234413.1"
                     /translation="MIKLIGKETLKVYYVAETKAEVSRWLLKMFNKTRANASVSQKAS
                     VSETGMPEAMWISRRN"
     gene            9318..9788
                     /locus_tag="LP524_001350"
     CDS             9318..9788
                     /locus_tag="LP524_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234414.1"
                     /translation="MAMIPKFRAWDREKDRMIYPSTTGVCFEMNDDGISVLDIDVDYP
                     NDYGFPKIASILMQSTTLKDKNGVEIFEGDIALITHKSTNYADTYWHSYVEVFRAKNG
                     AYRIRGEHIYETELYSNRKQLTVAGNVYNSPELLKRDRFMTTTENPELLKEEQR"
     gene            9785..10327
                     /locus_tag="LP524_001351"
     CDS             9785..10327
                     /locus_tag="LP524_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HBL2234415.1"
                     /translation="MNKQELIDKYTAEISRLRPYCPNRHLISDQLKYGLYKEILEDLK
                     QLDEPQKPAVPKFVAEWIEYAKKKGDSLAISFKPWNLYGVEYSKTDRWIEDNQGTFAR
                     AWLIGYEVEKEPLYYVKLPVVYFNHWDLETYLMKDDRGNITIADNNDFDDMKFTESEI
                     KAIDERYWPFAVPVEEVAEG"
     gene            10324..10707
                     /locus_tag="LP524_001352"
     CDS             10324..10707
                     /locus_tag="LP524_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234416.1"
                     /translation="MSNLKVGDIVFYKGDEGIIREVMTDGKENFYRVDVSRKSILYLY
                     EDELDLGTQKLNDNQKMVFDYLKSNVEQGGKSVMYSIFLLGDWDSRIGAARNVDIAYC
                     GLNGKQEAEVLSAFAQWGLEQEEAE"
     gene            10704..10934
                     /locus_tag="LP524_001353"
     CDS             10704..10934
                     /locus_tag="LP524_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234417.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDDTFRLGS"
     gene            10941..11144
                     /locus_tag="LP524_001354"
     CDS             10941..11144
                     /locus_tag="LP524_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HBL2234418.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            11141..11437
                     /locus_tag="LP524_001355"
     CDS             11141..11437
                     /locus_tag="LP524_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234419.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSVIIRIENTMECDKWTAKSKENLAVVRLVDIELINDK"
     gene            11514..11927
                     /locus_tag="LP524_001356"
     CDS             11514..11927
                     /locus_tag="LP524_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBL2234420.1"
                     /translation="MQLLREVAFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            11940..12326
                     /locus_tag="LP524_001357"
     CDS             11940..12326
                     /locus_tag="LP524_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234421.1"
                     /translation="MWKDYVSLKELKKDLIFKKIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHSSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKEKLCVVTDA"
     gene            12818..12890
                     /locus_tag="LP524_001358"
     tRNA            12818..12890
                     /locus_tag="LP524_001358"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12851..12853,aa:Lys,seq:ttt)
     gene            13276..13536
                     /locus_tag="LP524_001359"
     CDS             13276..13536
                     /locus_tag="LP524_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234422.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSVFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            13579..13752
                     /locus_tag="LP524_001360"
     CDS             13579..13752
                     /locus_tag="LP524_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234423.1"
                     /translation="MKQYTAKDFEEMKRLKKDYEEVDMELTVGVIQRRLRVGLETAKA
                     IYNDLNAIEEKNG"
     gene            14141..14974
                     /locus_tag="LP524_001361"
     CDS             14141..14974
                     /locus_tag="LP524_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HBL2234424.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFETLSDEEKDIYSSLNDNPSFVLSEEIRLLKIRQFRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKNPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            14967..16382
                     /locus_tag="LP524_001362"
     CDS             14967..16382
                     /locus_tag="LP524_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HBL2234425.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            16394..17923
                     /locus_tag="LP524_001363"
     CDS             16394..17923
                     /locus_tag="LP524_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBL2234426.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            17841..18893
                     /locus_tag="LP524_001364"
     CDS             17841..18893
                     /locus_tag="LP524_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBL2234427.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            18895..19212
                     /locus_tag="LP524_001365"
     CDS             18895..19212
                     /locus_tag="LP524_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234428.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            19339..19962
                     /locus_tag="LP524_001366"
     CDS             19339..19962
                     /locus_tag="LP524_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HBL2234429.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            19977..20867
                     /locus_tag="LP524_001367"
     CDS             19977..20867
                     /locus_tag="LP524_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HBL2234430.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLFGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            20890..21105
                     /locus_tag="LP524_001368"
     CDS             20890..21105
                     /locus_tag="LP524_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234431.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            21117..21458
                     /locus_tag="LP524_001369"
     CDS             21117..21458
                     /locus_tag="LP524_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234432.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            21458..21829
                     /locus_tag="LP524_001370"
     CDS             21458..21829
                     /locus_tag="LP524_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234433.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            21822..22217
                     /locus_tag="LP524_001371"
     CDS             21822..22217
                     /locus_tag="LP524_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBL2234434.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            22219..22596
                     /locus_tag="LP524_001372"
     CDS             22219..22596
                     /locus_tag="LP524_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234435.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            22597..23205
                     /locus_tag="LP524_001373"
     CDS             22597..23205
                     /locus_tag="LP524_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL2234436.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            23205..23546
                     /locus_tag="LP524_001374"
     CDS             23205..23546
                     /locus_tag="LP524_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234437.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            23788..26577
                     /locus_tag="LP524_001375"
     CDS             23788..26577
                     /locus_tag="LP524_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBL2234438.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEEFRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNEIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            26567..27307
                     /locus_tag="LP524_001376"
     CDS             26567..27307
                     /locus_tag="LP524_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL2234439.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYRNVTRKIECYYKAPSIDVVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            27304..>29141
                     /locus_tag="LP524_001377"
     CDS             27304..>29141
                     /locus_tag="LP524_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL2234440.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLAEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRLIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     SLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFTSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT        10360 a         4250 c         5923 g         8608 t
ORIGIN      
        1 tttcttaatt caccaatgta ttttttgttc gtcaacatat ttttaacact ttgataatct
       61 ctaacgattc catactgaga gcctaaataa taaacagttt ttcttaaact accactagaa
      121 agaaagtagt tgaatatatc tatcacgata ggagcatcat catttattac taatttctta
      181 ttttctatag agtagcctaa cggagttttc ccgcttacta cttcaccttg tgcaatttta
      241 tttgccataa cagctttgat gcgttctgaa tcaatttggg cttccaattc agcaaaactc
      301 attacttgtg caatgaaagt tctgccatac gctgtagttg tatcataata ttgttgagaa
      361 acagcattcc aagaaacatt gtgtttttct aaaatttctt gagtgtttaa ataatgtcgt
      421 agatttctaa accatctatc taattttgta aataaaatta gatctatttt attttctttt
      481 acatcttgta atagtttttg aaattcatca cgatttattt tttgaccaga aataccaccg
      541 tctacatatt cattaactac tttcaaatcc ttgtgttgtt cgatatattc atagagggtt
      601 tctttttgtt ctctaagact atctccatgt ttggcttgtt gttcagttga tactctcata
      661 tataatgcta ctcgtttcat gttatctcct ttcgaatgta tgttcttatc gttgtaaaag
      721 aaaagcccga aggctaatct ttttactaca catctgttaa ttctaatgat cttctgtatt
      781 gttcagcttg agctagttta gtaaaagata cagttccgtt ataattttct aaaacaactt
      841 tttcaatttc atcaagagat actttaaaga attcttttcg ttgattaact ttattgactt
      901 cgttttttct aaaatgcgaa tgtagttgat tttctaatgt tggtgcatct tctgaaaata
      961 tcattgcatg aacatcaaat tcaaaaggta cagaagcgct actaagttcc ttaattctat
     1021 ccataggttc taaccgtcta gtcattccta ttttatagac atcttcaccg aaagatccga
     1081 tattactgat gacataaaca tagccagctc tagtattaag ttctctttgc aatacatctt
     1141 ctttgacagt ttctagttct cttaatttct cttccagttc tttaatttta tcagcataga
     1201 gttctttttc aacatcagca ctagcttttt gcatgtatgc cattaactta gttatctctg
     1261 agttaaactg tcgttcttct ttatcaatgc gtttcttttc acgttctatt tcacgtctta
     1321 ccttttcttc ttcaatcatt tgttctttta ttgctttttg ctgttctttt tcatcgttaa
     1381 ctttaactat gtatgaatag ttgagttcta attcttgaag ttttaattca agtaattctt
     1441 ttttgaattc tgcttcatcg tttttaaaaa gcttgtttat tttatcaaaa gttcttataa
     1501 ttttttctct agttgaatct acgttagaaa tagtaagttt attgagaagt ccagttattt
     1561 ctgattctaa tggtataagc attttctttt taatattatt agcttctcta ttattcaaat
     1621 aagaaggaat attaattgca ccgtctaaaa taaaatcctt ttctttaatc tttaataaag
     1681 aaagtttgtt ttttatttcg gatgaagaag tttcatctat attcggaata attactgatt
     1741 tatagatagt gaccagagat gtgtcatatt tttgattaat ttcatcgtct aaccgctggc
     1801 tttcagcatt aagaatatca attttgtttt ctagcgttga caagttttta ttcaatttac
     1861 ttatgttttt ctctttttca gatataaacg tttccctagc atttaattct tgagaatatt
     1921 cctttacctt actctctaag gcagaagttt tatcttttaa ttttttagct tcagataatt
     1981 tgctactacc cataattatt aaaactattc caactaaaaa caatagtact cccattcaaa
     2041 acgcctcgct tcttttataa aataattgtc aaaatcatat aaacccttac cgaagttgac
     2101 ggctccggca tgggaatttt tttaaaatac aacttttccg ataattctaa tctgttctgg
     2161 cgatacaatt ctatcttcgt ataagtcgtt tatagaacgt aaaatgattg tttgattttc
     2221 gtaatcttta tatatctctt tacatgtaac tccgtcacca cctaattcca caatagcaat
     2281 ttccccatct tctacgtccg gttgttcttt aacgaatacg aattgtccat tcctgataac
     2341 aggttccatt gaatcgccta taataggtac tgcgaagtca gcagcacttg gaactctctc
     2401 aaattcttcc tcttcgacaa ccgtatcacc gtaagtaagt tctgttggat tggcagccga
     2461 agccgaattg tgaacagttg ggattttttc ttttttagga aaatctagga cattttcttg
     2521 tttttgttct tctaatcgat tacttgcaaa ttcataggta gcaactttat tttcgtcgtt
     2581 aagctgatta tatataggaa ggatattata tatctgatta gatatagata aatcaatatt
     2641 catcaaatca tctatatcaa cattgaatat cttagctatc tcatttaagg tttttaattt
     2701 tggtgtatat tttccttttt cccattcgct tattgaggat gaactttttc ttcctagttt
     2761 ttcggctagt tcttgttgat caatattata cttttctcta agaaacttta gattttcagc
     2821 gaacatagta acacctcctt gagttaattg taacaccgtt tcggaaaaaa tgtaacaggt
     2881 ttcttagaaa atattttagt gtttttaaag aagtatttac gcaaaataga aatcgatttc
     2941 aggaaaactg aaatatgcac ttgacttcgg aaaaaccgaa gtatataatt tagtcagaaa
     3001 gttaaagaga ggaggcgaat aaaatatgga taccgcgaag aaagaaaaat atacattgag
     3061 acaattaaga gctttaaagg gttattcaaa agaagaactg tcaaggaaaa gcaaagtgac
     3121 atcaagaaca atatttattt acgaaaacga tgtagataaa atgaggaacg gaaagtacgc
     3181 cactctagaa aaaattgcta aggcattggg tgttagagtt tccgatattt ttttagaccc
     3241 tgattcggaa aaaccgaaat caataacagg tgaaacaaaa caagtcagct aggaggtgtg
     3301 aatagtggaa gagaagaata taaaagtacc gactcttgaa gaatatacaa aaattgttgt
     3361 cgaaacagac gagcagaacc cgaaaacaat cgcagttatt acggcgaatg atattgaatc
     3421 atgcgaaggt tttcgagttc gaatgacccc tagatatgat taatgttctt tatcacgtgg
     3481 aggtatagga tcgtttccaa aactatcttt tgattgtatt tgtccattct ttttttggac
     3541 aataaactct gattgctgtt ttttagaaat ctttttacca tactcaacag catctgattt
     3601 attatcaaat agttttgtat cttcggaatt accttcacca cgtacagccc atttgccatt
     3661 tctaggagat acccattgat ttttacccat aacaattcac ctcgctttca agttaattct
     3721 attaagaggt gaaaagaaaa caaactgatg gaggtgagat tgtgagtaac aagccaaaaa
     3781 tcactttgaa tggcacacct aatcttcaaa gattagcaga tgaaatggct agaatacgtt
     3841 ctagacattg gggagttact gtggtggcaa ccataattga acaaaaagaa aagcccacac
     3901 agccggcaag caagtagtgg gcaccataga acaataatat tatatcacac actcgccact
     3961 tccgaaaaga ggagttgctg ctctcctttc aagtttgcca aagtccacgt ttcttcattt
     4021 tggcaactcc tcttcttgga ggtggtgacg gcgagagacg aaggagggat gacatgaagg
     4081 aagaatcaaa aatagggatt ttgaatgaga acaattacac aagattaatt attgaaacag
     4141 aagaagggga aaaagtagca gaagttacat tgaccgatgc aactcctgct gacggttatc
     4201 gtattcgatt aactcctaaa tatgattaac cttttggtgg gtgtggatcg tttccgtgac
     4261 tatcacggct ttgaattttt ccatctctgc catgaataaa tagctcagat ccttgatttt
     4321 tagaaatctc tctagcgatt tttactgctt ctgattttgt attagtatgt gcagtcgctt
     4381 tagaatttcc agcacctttg acattccaac caccattgga attaggtaca acatgttgat
     4441 tttttcccat agttttcacc tccatttcta tagaaatatt ttatcaaata gaaatggaga
     4501 aattcaataa acgaaaggag ggatcaaatg acattacaag agcatcaaca cttgatgcga
     4561 aaactaaatc aggaatacca cgaggaaatg aaacctagat tgctagggga acaatggcgt
     4621 gaacgccaga agaagtggtt agaaataaaa aagtgactca gccgtccaaa gcaatgagtc
     4681 acaaaccaaa aattatacct aaggagattt tatcacatga ataagaaaat tgaaaatctt
     4741 atcgaagaat tgaaacgtga atgccaaaag caaggtgttt ctattatttg tacagcccaa
     4801 aaagaagggg aacttaaaag tctcgtttat ggtgagacaa cagaaatttt actttgtctt
     4861 gctatgcaag aagaacattt agatgaaaat ctcccattgt cagcacatat tatgcgtaga
     4921 attgctgtgg atgcttacga acaagcgaaa aatgaagaag aaaatcaacc ttcaaatcat
     4981 acttttgtta ttaataacaa ggaagattta gctgatgtga tgactcgtat tctcaagggg
     5041 gaatttcaat gatgaatgaa aaaacaattg atctactcat tattttattt ttcacgttag
     5101 tgattgtagc gaaaagttat gtaatctttg gcttgttatt cgcaattacc atgcttgtgc
     5161 tttgcgtgtt agcaagcaga aaagaggtta aacatgaacg agttagcaaa tcttgacaac
     5221 tatttaactg atcctgatta tgctaaacca ccatatgaag cgccaattga tgaggaggat
     5281 gaagatgagt aaatctacct tagaaatgag ccatcaagaa tggctcgaag accgcaaaag
     5341 aggtattggt ggctcagatg ttgcaaccgt acttggatta aacaaataca aatctcctta
     5401 tcaattgtgg cttgagaaaa cgggtcaaat tgaattgaaa gatttagaaa gcgaaccagc
     5461 ttattggggt aatgttttag aagaagtggt tgctaaagag tttcaggaac gtacaggcaa
     5521 aaaagtacgc agaagaaacc aagtatttga acatccatta catccatttt taagagcaaa
     5581 tattgatcgg gaagtagtgg gagaaaatgc cattctggaa tgtaaaacag ccaatcaatt
     5641 tctcggaaaa gagtgggaag gtgaagaggt accgcttagc tatctctgtc aagttcagca
     5701 ttacatgaac gttttaaaca aagactattg ttatatcgct gttttgatcg gtggacaaaa
     5761 attcatttgg aagcggattg aacgagatca agagctgatc gatacaatca ctgaacaatt
     5821 agtagagttt tgggaaacga acgttcttgg aggtatcgag cctgtaattg acggaagtgg
     5881 agcgactgct gacttcttaa aagaaaaata tgcagatgta gaagaaaatc aaacagctct
     5941 accatcacgt tttgatgaac ttatcgagca aaaaagagaa ctcaagcgga cgaaaaaaga
     6001 aattgaatca tctattcgtc aagtagacaa cgagatcatc agtgagctag gtaaacgtga
     6061 agccagtatc ggtatcacac aaagaaacat tatcagctgg aaacttgttc gtacaagacg
     6121 gatgaacacg aagaaactag cagagaaata tccagatgtc gcaaatgatg aagagattta
     6181 taacgttact gaatcaagaa ggctaaccga aaaggagatc aaataatatg gcaacaaatg
     6241 aatcgttaaa aaatcaattg gcagaaaagc cacagaaaca agttgcacca ggacagttag
     6301 ggcttaaagc tctaatgaat acaccaacaa tgagaaagaa atttgaagaa gtacttcatg
     6361 acaatgctaa tgcttttatg tcgaatgtta tgactcttgt atctaatgac agttatcttg
     6421 cagatagtga accgatgtct atcatgagtg gtgcgttaac tgctgcaaca ttaaatcttg
     6481 ggctagataa gaatttaggt tatgcatatt tagttccatt caatagtaaa aacaagcaaa
     6541 caggaaaatg ggaaaagaaa gctcaattca tgcttggcta taaaggatat atccaattag
     6601 cccaacgatc aggtaaatac aaagcattaa atgtgatcga agtttacgaa ggagaactaa
     6661 aaagctggaa ccgactgaca gaagagtttg agtttgatcc aaatggtaga acgtctgatg
     6721 aagtcattgg atatgttggc tatttcgagt tactgaatgg attcaaaaaa actgtctatt
     6781 ggaccaaaca agaaatcgaa gctcatcgga ttgctaacaa taaagatcgc gataagacaa
     6841 agttaagtgg tgtgtgggca tctgattaca atgcaatggc acgaaaaact gttttgagaa
     6901 atcttctttc taaatgggga atcttgtcca tcgaaatgca agaagccacc acatcggatg
     6961 agagagtcca aagggttcaa gaagacggca gcattattgc tgaaacagaa gttgaagaag
     7021 atattcctga aagaaaagaa gcagaggtta tatctgaaga aaatgaagat gtacaaactg
     7081 gattatttga tgcatctaat ccgccgttaa acaaataatg agggagcttt ctcccttgct
     7141 tttctagagg agaaatacga atgaacacag gatatgtcaa attacatcga aaagtgatga
     7201 attcattcgt ttggaccaat ccttatatgt ataaattatg gagcttgtgt ttgatgaaag
     7261 cgagtcatga gaatcgcaag atacttttta atggaaaaga gatacaagtg aacagcggag
     7321 aattcgtcac agggcgcgat gctatcacat ctgagatgaa cgaaggcgtg aaacgtgaac
     7381 atcaagtgaa cagcggttct gtatggagat ggctaaaaca atttgaaaaa aacggaatgt
     7441 tgaacatcaa atcaactacg aaatacagcg ttatatcaat aaaaaactgg tctttgtacc
     7501 aaagcagtga acaacaaatg aacatcaaac gcacaacaag tgaacaacaa gtgcacacaa
     7561 acaagaatga aaagaattat aagaatgaaa agaataataa aacatcatcg tcgcaaccac
     7621 gcaaaaagcg tgtttacgac accgactcag tttactacat tctcgcggag gagttattca
     7681 aacagatttg tcagaatcag gaaatcaaaa agccgaatct gcaaagttgg gcagataaca
     7741 ttcggaaaat gatcgagatc gacaagcgga ccgaaaacca ggtacgggga atgattgaat
     7801 ggagtcagca caatgtgttt tgggcatcga atattttatc tgctaaaaaa ttgcgagaaa
     7861 aatacgacac aatggcagcg caagcaaatc gtgattataa aacaaaacaa actaaaacgc
     7921 ttgaatacga gaaatttagc acagatgagt tgcctatttg agaggaggcg taggcatgga
     7981 aactgttggg gaaatcatgg aaaagctgat acagaaagtg cttgtccaac gtggcgaatg
     8041 tcctgaatgc ggacagcctt tgtatggatg gcgtacgaag aaccctgatg gctcagaacg
     8101 ttgtaagcca acctgtatgc aatgtgggta taaagcattg cgagttcagg aagacttaca
     8161 aacagaacga atttacaacg agagcctgaa agcaagagca atcaattttt tcaaaggtgg
     8221 ttctgttgtg cctaatcaag cgttgtttga ttgcacattg cagaattatc aaattgtcga
     8281 tcaagaaaca agacaagcgg ttgaagtaac caaacgcttt gttaattcag tcttgttagg
     8341 aaatccaagt caccttgttt taactggaaa acaaggaaca ggtaaaagtc acctagcgat
     8401 ggcagcggct tgggaagtct tgaagcgatc aaactacgat aaaaaaatct tatttatcgg
     8461 gttacaggaa atgctggatc aaatcaaatt ttcgtacaac aatcctgaac tcagaaaaac
     8521 gattgaggga tcgttgattg cagatatcaa aacagcggat ttagtcatca ttgatgatat
     8581 cggttcagaa ctaggaaaag atgcatcaga tagtcgagcg tttggcataa acacgctaaa
     8641 ttcgttcttg gatgcacgac agaacctagc aacgattatc acaacgaact tgcttggtga
     8701 agaactgaaa aaagcttacg gtacgagaac aatatcaaga atgtttgtca actctgatgg
     8761 atttacgatg gtattttctc aaacagcaga caagcgcata aaaccagtca aaggagcgat
     8821 cgcatgaatt tgactaaaca agaaaaaaga atctgtcgtc agataatgtt gcaaattata
     8881 ggtgatcgtg acatggacga attatctcaa caagagagtc gaatggttcg gaagataagt
     8941 aagctaatcg gtgcagatac gatggatcga tccaagccat tacccaaact gaattttgaa
     9001 tcatttacgt ttgagaaata tagccatctg agagaggtag gctatagtgt aaatgctatt
     9061 cgaaaagcac ttggggtagg aagtagtgta tgggcaaaat ggcggaaaga aaatgtaaat
     9121 gaggtaaatc aaaatgatta agctaatagg caaagagacg ctcaaggttt actatgtagc
     9181 ggaaacaaag gcagaagtca gccgttggct attgaaaatg tttaataaga ctagagccaa
     9241 tgcttcagta agtcagaaag caagtgtatc agaaacaggc atgcctgaag caatgtggat
     9301 ttcaaggagg aactgagatg gcaatgatac cgaagtttag agcgtgggat agagaaaaag
     9361 acagaatgat ttatcctagc acgacaggag tttgttttga aatgaatgat gatggtataa
     9421 gtgtgcttga tattgatgta gattatccca atgactatgg ttttcccaaa atagcttcaa
     9481 tcctcatgca atctaccact ctgaaggata agaatggtgt ggagatattt gagggggata
     9541 tagcgcttat cactcataaa tcaacgaact acgccgatac ttattggcac agttacgtag
     9601 aagtttttag agcaaaaaac ggagcctaca gaattcgcgg tgaacacatc tacgaaacag
     9661 aattatatag taatagaaaa caattgacgg tagcggggaa tgtttataat tcaccagaac
     9721 ttttgaagag agatcgattt atgactacta ccgaaaatcc agagttattg aaggaggaac
     9781 agcgatgaat aaacaggaat tgattgataa atatactgca gaaatatcta gattaagacc
     9841 ttattgccct aatagacatt taattagcga tcaacttaaa tatggtcttt ataaagaaat
     9901 cctagaagat ttaaaacaac tagacgaacc gcagaaacca gcggtgccta agtttgtggc
     9961 ggaatggata gaatacgcta aaaagaaagg cgatagtcta gctatttcat tcaagccgtg
    10021 gaacctctac ggtgttgagt atagcaagac tgatagatgg attgaagata atcaaggaac
    10081 gtttgctcgt gcttggctaa ttggctacga ggtcgagaaa gagccgttgt attatgtgaa
    10141 gttgccagtt gtgtatttta atcattggga tttagagacg tatctaatga aagatgatag
    10201 aggaaatata acaattgcag acaacaacga ttttgatgat atgaaattta cggaatcaga
    10261 aataaaagca attgatgaaa gatactggcc atttgctgtg ccagtggaag aggtggcgga
    10321 aggatgagca atcttaaagt tggagatatt gttttttaca aaggtgacga aggcatcatc
    10381 cgagaagtta tgacggatgg aaaagaaaat ttttataggg tagatgtcag cagaaaatct
    10441 atactctacc tgtatgaaga cgaattagat ttaggtactc aaaaactcaa cgacaaccag
    10501 aaaatggtgt ttgattatct taaatcaaac gttgaacaag gtggcaaatc agttatgtat
    10561 tcgatattcc ttctaggtga ttgggactct cgaattggag cagcaagaaa cgttgatata
    10621 gcttactgtg gattaaatgg taagcaggaa gcagaagtcc tatcagcatt cgctcaatgg
    10681 ggattagaac aggaggaagc ggaatgaaat acgaaatacc actaagtgaa gcagacgtcc
    10741 agtcaattat taacggtcgg gaggttaata aaaaacttcc tgatggtact gaattagtca
    10801 tcagacaaag ttatttgaaa gatatggcag ctccagtatt aattgatcgt tttaacgtga
    10861 ctgattctgt ggtagagaac catttaaaag aatttcgatc aagtatagac gacactttca
    10921 gattagggag ttgattgaca atgaacaaca gacatcgcag aataacaaaa ctaagaaaac
    10981 aggaactgaa tgtactaaag acaaagtttg aaaaagaata tggaatttca gcagaagaaa
    11041 catataaagt ggcaagtcag tgtgttgctg atgcgagtaa tgctattcgt aagtttggga
    11101 tttcgatatt aaatgatgat cgtaaatggg aggaaatgag atgaaactaa aagacggatt
    11161 ttacgctagt agtcacggca tcggcggttt aatgctagat atgccgacaa agaatcctaa
    11221 aacacgtaag aaaccaaaag tcaaagtcgg tgacatggtt cgctgcgaag cagaagggtt
    11281 catctatcca tttcgtggat atgtagaaca cgtctataat cactcagtaa tcattcgtat
    11341 tgaaaacacg atggaatgtg ataaatggac agctaaaagc aaagagaatt tagcagtggt
    11401 gagattggtg gatattgaac taatcaatga caaataaaaa agccggatcg ctccgactga
    11461 ttcaataaat tccacaagtt tattatatca cataaaaggg gcggtttgac ttgatgcaat
    11521 tgttacgaga ggtagctttc aaacagacaa gatgtaatgc gagagatgtg ctgaagaact
    11581 ttcggcgttt ggagcggatg gcaggtcgct ctttgataga tattaagtcg ccgattatta
    11641 cggacatgcc gaaggcaccg aagcacggca ataaggcaga agacgcgatc attcagatga
    11701 tggatataga agcagagaga gacgcgattt tagcggcttt gatggcgctt agtctgatta
    11761 gtcgtcagat actctactac agcttttgtg tgccagatag cttctcaaac tacaggatta
    11821 gccgtgaagt gggttattca gaaagaagca tacaacggat gaagtcggaa gctctgatag
    11881 agtttgcaga agcatataaa catgggaaaa taattgctta taaataggag gactatataa
    11941 tgtggaaaga ttatgtatca ttgaaagagt tgaaaaaaga tcttattttc aaaaaaatcg
    12001 tagaatggtc agagagtgaa ttaattttag aagatggaac caaaatggaa gttgtatgta
    12061 gcgaatcaga ttgctgcgca tgggccgaag gtgaattcaa aaatgtaaag cttgatgcag
    12121 tgattacaga tataaagatt tttgataaag gtaaccgtct ctataatggc gatggacata
    12181 gttcttatgc cgaagtcgtt gtttatcata atcgaaatga gattgctaaa gcggaatgta
    12241 cagcaaacga tgggaatgga ggctactatt acagtgtctg tgctttaaaa gtcaaagaaa
    12301 aactttgtgt agtgactgat gcataaaaaa tggcggtttt ttggcggaat gatggcggtt
    12361 tttagctatt taccagtgat attatggtag tgtcgaaaga ttagtgatag gtctgagaca
    12421 aaataaaatg taagggagga aatctccctc atcgtttaat taagcttcga tagacagcag
    12481 cggaaatatt aagaataagg atgtgaattt taactccttc taaattgttc ttattatcta
    12541 tcatccgttg ctgtctattg ttattatgtc actgtggcgg aaagggtaga cgcttaaaaa
    12601 taaggtcaat acgtcgaggg atagccttaa cgttttatga tttgaccatg caaggttcga
    12661 ttccttgcca gcgacttaga cgaaaggtag cttaaatatt aaccacatca acatcgtcag
    12721 cgagttgata aaggtaacta gcagactcta ggatttaagc cagtcttacc gattagtctg
    12781 ttatagtagc aagcttgcta gaggtagctc ctctggttga cgtgcagctc agttggtaga
    12841 gcgcctgact tttaatcagg aggtcgccgg ttcgaatccg gtcacgttaa taaagaacct
    12901 acggaaacaa tcaatcttat cggatgccga ttggttggct gaattaagca gtgtgtctgt
    12961 gaatcagtta ataggcataa aatactagca cagacgtgtg ccactctcag gtgtaggtta
    13021 ggagagaaac attagcaacc gagggttgga aatgggcgct caaagtacac gagcaaggcg
    13081 aggtcgatag taatcgatgg aatcggtgta ggttgctatt acataattga ttgggttaga
    13141 ttgagttttg ggattcggta caaatgaatc gtcaaatgac tcaagcacag gatcggaaac
    13201 gtccctgcct gtgcattaca tattagatca ctcgttgagt ggtcttttta ttttagaaaa
    13261 gagaggattt tgaaaatgaa tcatgagaag tttatcgaaa aatgcaaggc tattgtacgt
    13321 gaaagaattg agaatgagat tgctgaccta agtggagcag tacctgagtt tagtgttttc
    13381 atagtctggt catgcaaaac actacagaat agcaaagcat tagtcagcgc tagcttaaaa
    13441 ggagcaccgt attttgaaat tacgttgaac ggggacaaag gtgaaatcta tgtagatact
    13501 tatctcaaaa aatcaaatga atgtatcaaa gtctagcagg tgctaggctt tttctttaca
    13561 taaaggagga atgatctcat gaaacaatat accgctaaag atttcgagga aatgaagcga
    13621 ttaaagaagg actatgaaga agttgatatg gagctaactg ttggagtcat tcaacgaaga
    13681 ctgcgggtcg gattagagac agcaaaggct atttacaatg atctaaatgc tattgaagag
    13741 aagaatggct aatgaggaac tactggtata tatcgctaac taatgaatat cctcgaacca
    13801 ttgatgattg ttcagtgcgt gttgtgcgtt ctgtacaaat caaagggaag tactctattg
    13861 tcgaaatggg gagagaagca gaaccatgtg agattgatgc gtgcaagctc gtttattgcg
    13921 gtcatggttt ctatgatgaa ccaaacattc aaaataatat taacaagaat ttgagggatt
    13981 agaatgcaag aaatggctta aaaatctcca atcaggacaa atcaagttag acaaagtttc
    14041 cgatttaaag atattaattg aagcagatct aatgttgaaa gatattgaaa attagaaaac
    14101 aaaactcaac ctaagaagat tgcgaggtgg tgtgtattga atggcaagac aacgtgatcc
    14161 aagacgtgat gaagccaaaa gaatttggtt agaatccaac ggagaaaagc agttaaagga
    14221 aattgcatct gaattaaatg tttcagattc tcaggttaga aaatggaaat cgcaagacaa
    14281 atggagcgct gaattgaaaa gtaacgttac caatggcaaa agtaacgtta ctaatcaagg
    14341 tggcgctcct attggtaatc aaaatgctaa aggtaacaaa ggaaatagcc gagcttctcc
    14401 gccagtgggt aataaaaacg ctttgaaaac aggcgagtat gaaaccatat tttttgagac
    14461 actaagtgat gaagagaagg acatctattc tagtctgaat gataatcctt cttttgtttt
    14521 gtctgaagaa atacgtctac ttaagataag acaatttcgt atgatgaaga gaatccaaca
    14581 agctgaagct ggactaaatg atgaagaagt cgaacgattg cagcagctaa gaaagattaa
    14641 aaatccgatt gaaaaaaatg gtaaaaagct agaaatcaag cgtgaggtta tgcaagatgt
    14701 gcagattagc agaaaaaaac atcgcaaaat tgatgatatt ctttcaattg aagattcatt
    14761 gactcggatt agcaaccagt tagctaaagc catcaagcaa atgaatgaac tttatatgaa
    14821 tgaatacaga actgatttaa ttaaagctca gactgataag atccaagctg agacaaatga
    14881 aattggcgga aataattcag gtgaagaaat agaagaatgg aaacaggcag ttttaaatgc
    14941 cgcaaacaaa cgggcggtga aagaaaatga ataatgaatt tattcctttt gctgatattg
    15001 gttctgccat tgattattac tatgataaac cagtagcttt ttgccaagat attttgcatt
    15061 tgaatcctga tgaatggcaa gaaaatgttt taaatgattt agctgaattt tcaaaggttt
    15121 ctgttcgttc tggtcaagga gttggaaaaa cagcattaga agcaggagca atactttggt
    15181 tcttaacgtg tcgaccctac gctaaagtaa tagcaacagc tccgacaatg aagcaacttt
    15241 acgatgtact ttgggcagag gtagctaaat ggttaaatga tagcttgatc aaaaacttac
    15301 tgaagtggac aaagaccaaa atttatatgg ttggtgattc agagcgttgg tttgctacgg
    15361 ctagaacagc gactaaacca gaaaatatgc aaggttttca cgaggaccat atgttgattg
    15421 tggtagatga agcttctggt gtgtctgatc caattatgga agctattctt ggtacgctat
    15481 caggttttga taataagctg ttgatgtgtg gaaaccccaa taatattgaa ggtgtttttt
    15541 acgattccca caattcagac cgtgataaat acagagttca taaagtatca agctatgata
    15601 gtaaacgtac aaacaaagac aatatagaaa tgattcttaa aaaatatgga aaagaaagtg
    15661 atgttgctcg tgtccgtatt tttggagaat ttcccaaagg tgcgttggat tcatttatca
    15721 gtcttgaaac ggttgaattg gctacagaaa aacaaattag tgattcttta gtcaataaaa
    15781 caacggttgc tcatattggt gttgacgtag ctcgatatgg tgatgattct acgattctct
    15841 ttcctagaat tgctaccagg gcattggagt atgagaagta ttcaaaacgt agcaccatgg
    15901 aaacaacagg atatgtcatc aacatggcca agaatctaat gagtcaatat ccgagtattg
    15961 ataaagtgat gattaaagtc gatgacactg gtgtcggagg tggtgtaacc gaccgcctag
    16021 aagaacttat agaagacaaa cattatcctt ttgaggtgtt tggagtgaat aacggttcaa
    16081 catcagaaga cgatttttac gataatttag gtactcaact atggggaaac atcaaggaaa
    16141 tgttagaaga aaatatgaca gcaaatctta acggagaaca gcctgttatt gaattgcctt
    16201 ctgatagttc gttaatcaaa gaattaagta ctcgcaaatt caaaatgaca agtagaagtc
    16261 gtatacgttt agaaagtaaa gatgatatga aaaagcgaaa tattggtagt cccgatattg
    16321 ctgacgcact ggctttagcg ttttatgagc caccaagtca ctatcaattt attcaatttt
    16381 aggaggtgag cttttgatta gtacagtatt atctttagag agatataaga agctacgtgt
    16441 taaatatgca acacaaattg aagatggaat gtttgatccg aatggtttta tagaagatat
    16501 gaaaccattt tttgctgatc gcgaaagaaa atatctagct tatactagcg aaaagaatga
    16561 aatagataat agaccaaagc ctaacactga tattgtaaaa gttaataata aacttcatgc
    16621 tggtatgtat tcaattgtcg tagaccaagc agtcaatcat tttactggta tacctatcaa
    16681 atgggattac gatgtatcgg aacaaaaaag aacactcatt caaagattaa aagataaatt
    16741 cttaaaaaat gatatagaac ttccgacagt tccagaagct tttaacaaat taacaagcaa
    16801 tcttgattcc atgagattcg caatgcttga ttctgaaact gcaacttttc aaggagcttg
    16861 tggagtagct ttccgattgt tagagcctgt ggaagaagat gatggttgga agttaagagc
    16921 aagtaatatt gaaccttgga gagcagaaag atatggaaat gccggaatct atatcaaaga
    16981 aaagtatgac tcttaccaga aaaaattttt tcaagaaatg aaagttatga caagaaataa
    17041 aattctgaca tatgcttgct atggtgattt gaattttgtt acaagcggaa cgttcaaaaa
    17101 aattgatgag accgataacc ctttggggac gattaccttg gcagaattta aaaataatac
    17161 gaatcgttat tgtgattttg aagtagctga ggaaattgga gatgcaattg atcgtgcttt
    17221 gtctgatcaa caaaacgaaa tcgaacaatt taagcttgcg tacatgcttg ttactggcac
    17281 aacaatgagc aaaggcactg ctaaagaaat gatgaatcag ctagggatta tcaacttaaa
    17341 agaccctact gcaaaagctg aatatgtcac aaaaaattta gcaaaagatt tcaatgaata
    17401 tcatatggat ctattgaaaa aacagtttta caccatttgt aaagcaatcg attttaacga
    17461 tgaggtgttt aaatcaaaca gttctggaga agctcgcaag tggcaaatca ttagcctaga
    17521 agcgaaaaca aatactaaag agcaatattt tagagaagga ttgaaagaat gcgcagaaac
    17581 aattgctgct tttcttaagt ttcatgacaa agtagaaatt gagcctgaaa aaattatttt
    17641 tactttctct agatctttgc caactgattt aagttattta gctgaagctt tacctaaact
    17701 tgcaccatac gtatccaaac gaactattca gagccaaatt ccatttgtaa cagatgttga
    17761 ttatgaaaat gaaatgatgg aattggaaag tggaagtgct tatccagata gtgaatacaa
    17821 ttttggcgga ggtggcaata gtgacgatag aaacgaagta ttggaccaaa cgtcgagaac
    17881 tggaggatca agcaaggctc aaacaagaaa atcagacact taaaaaatta actagtgtat
    17941 ttcctgaagc actgaaagag atacaagcaa aactattatc acaagctgac ttacacaata
    18001 tcacttatcc agaaatgatg gagttttata gtacaagtaa tcagaaaaaa tatcgtgaat
    18061 atgtggaaaa aaattataag tcattaaaaa tgtatgatgc aaaatacaaa gagtttatcg
    18121 atgaattttt tccaccattt gactatgcaa aagtcaatcg cttattacaa atacgatcag
    18181 atgtatttaa aattcttgca gaatatgcga tggatgcaga tgtgaatcaa tatttttctg
    18241 atcgcttaga ggaaattctt caaagaacat attcttctaa tgctaatgtt tttgttcaac
    18301 ttttaaacgt tgatatacca aattatttac cagaaaatga aattaaacat tatttgaact
    18361 atccatggtc aggaaagaca ttttcaagaa gactttgggg aaatatttca tcgcttgaac
    18421 agaaactctc taatgctatt gtaaaaagtg ttgctagtgg agaaggcgtt atacacgcat
    18481 taaacaccat gagattagac tcagaaattt gtgacatgtt taagttagaa gaatcaaagt
    18541 ataacaaagc gatagaaaat ctcgttcgaa cggagtatgc aaaatttgca caagatggta
    18601 ttgaaaaatc atatttagaa acaggtattg aggaatacaa cgtattgact gcaaaagatg
    18661 agagagtttg ccggatttgt ggaggaaagg caagtaagaa tccctataaa ctgaaagatg
    18721 ctgtcatagg tgaaaatcga gcacctttcc atagtcgttg tagatgtacg gatgttccta
    18781 atttaccaaa attaggaaag gatattgatg aagaatatga ccgtttattt ggcgatttat
    18841 tagatgagtt tgcacatgat tcttttggaa ttaatttgaa acggaggaag tagaatgaaa
    18901 gatttttttg aagcagtact aacaattaat gtaaatgctg atattgcaga agcctacaaa
    18961 acagctattg agtctgagaa ccatcctaat ggcttgagag accattggaa tggcaattat
    19021 gcctacgtgg ttattggcga tcaaactgtt aattatcaag ataatactcc agttgataag
    19081 aataccgtta atttaacgat tcaattatta tctcattcat taccaaattt aaaagaaaca
    19141 gttgattggt atgaaaagat gggatgtatt gttgttagaa ctgactacaa agaaggaaag
    19201 tctagtaatt aggctttttt attttgtccg aaatgacgtt aaactagcgc aatactgggc
    19261 ttggttgaat ggtggggcgc aactattaaa actcaaagca atgcggggcg tgaaaacgaa
    19321 tcgtggggcg aaaggagaat gagcatgaaa cacaaatcat taatgccaat gaatttgcaa
    19381 tactttgccg aaggtgacga tcaaaagttt tcttttgatg acttcaaatc ttttgtggaa
    19441 tccaatgaag aagctcaaaa atttattcag tcacaatctc aaagtgttgc cgacaaacaa
    19501 ttggaagctt ggaaacaaaa taacttagat aaaattaaac aggataccat caaggaatat
    19561 gaggaatcta agaaaaacaa gtcacctgag caaattcaac tggaaaaact acaagctgaa
    19621 tttgaagcag aaaaagcgtt gcgtgtgaca agtgataata aagcatttgt tgcagaacaa
    19681 attgctggat tagaactaga tggagagtta aaagagtcta tttctcaatt tatgctaaat
    19741 aatcttgtta gttcggatac agatttcact aagaatgctg ttgaaggttt tacaagtgtc
    19801 ttgaatgcaa ttaatgagaa acatgcagat gcattaaaag aactacaaat gaagtctgca
    19861 tttggtggaa ctcaacaatc gaataaccag gttcagcaga acaatgaaac attaacaaat
    19921 ccagaagaac aattaggaca aattcttcaa caatttaact aggagagtga aaaattatga
    19981 aaaaaacatc tttaaataat ttagagtatt tggatatttc accagcgatt aatgctatgc
    20041 aagtaccaaa tacacctttt ttaagctatt tatttggtgc tggaaaaaca gagccagcaa
    20101 actcgacaga aattaaatgg cgtgaatatg atatcaacaa cgatgattct tctgaaaaac
    20161 ttgagggcgg agaatatcca gatgctgaat caggtcgaac ttggtttaac aactatactg
    20221 aaatttttag aaaatcaacc tctgtatctg gtacattaga tgctattaat gtgaatggag
    20281 tcggaaatga attaactaat caagtagcac tacgtggtat ggaaatgaaa attgatttga
    20341 accgaaaatt gattactggt gtaaaagctg atgaaaatgg ttctaaaggt cgtcgaatga
    20401 atgggatttt gaacttgatc aattcagcaa ataaggcaga aacagccact gcgggtgcag
    20461 taacaagaaa agatatcgat actttattta aattgatgta tgaaaaaggt tatatgggag
    20521 aaaaactatg cttgatttct ccagatatgc aggagttaat gactgatgag ttagatggaa
    20581 aatcaacaaa aattgttcag ttcggggaaa gagtaacttt tggattgcaa attggaaata
    20641 tcgtgtctaa ttacggtaca ggtattgctc tactagaacc atcattgcca aaaggaacaa
    20701 ttgccgcaat cgatactaat tatgtgaaac tacgtccatt acgtgaatgg agagcagaag
    20761 aacttgcaaa aacaacggat tctagacgta tcggtcttgt aggtgaatac tctcttgaat
    20821 acaatgcttc aaactctggg gcaattttaa atttaaagtc tgaataaaag ggagttagta
    20881 ctcccttttt tgataggagg aattacagtg gtaaaaaaag atgaaactaa aaaagatgaa
    20941 gtcgtgaaat atagagtagg taaaactaaa aattttgttg ggtttgttca tcctaaaact
    21001 cgtagattta tcacagcaga ttcaaataac gaatttatca tttctataga tgataaagaa
    21061 gcaattgcaa ttttggaaga tgcaattgat gttaatgaaa tttaggaagt gatctgatgg
    21121 atgaatcgct aaaaacggaa atcattgagt ctacaaaaga agattttcca gatttgagtg
    21181 aagaacgcat aactaattta ttagaaataa ttttgctaga aattgaatca tacaacactt
    21241 gtaaaaatga tatttcatgg gaaaagttaa aaagcgtgat tagtgaagtg ttgtataaaa
    21301 taataaaaaa tgaatcagaa aaaacagtat cttcagttag acgtggcgat acaacgatta
    21361 gttatgcttc aacaacaaat gatgttagtg agttacttct aggctatggt gatttgatac
    21421 gaagagttat tggttgtgga ggattggagt ttttttaatg aatgaagcag atattttaga
    21481 aactacttat gaagatagtt gtattattga aagactgacg gacattgaag attctaatac
    21541 aaatattact attcaagatt acaaaaaagt atatgataat cctatttctt gcgctctttc
    21601 acaaggtcaa atcgatggac tagcagtcat agaagatgga gagatggtaa atgtttcaac
    21661 tgacacatat aaattatttg ttcatcctaa gattaaacta aaaaaaggag atcgaataac
    21721 aataactcaa aaggccagtg gcttaatttt ttctctattt gctactaagc ctttttacta
    21781 tcctagtcat tgtgaagtga atttgatagg aagtgagaaa aatgggtaat ctcaaatatg
    21841 aatcaaatgc agaaaagatc attgagaatt ttaaaaatat gactgtaatt gctcaaaaag
    21901 aaggaatatc cttcgttaac gattcaatga ataaagtcgt tagtttagct aaaccattaa
    21961 ctcctgtaaa atcaggtaat ttaaggcgag gatatcgtgt agtaaaagct agaaaactat
    22021 caagcggtcg aattgttgga gcagttataa acaatgaaca ttattttaaa tatgtagaag
    22081 aaggtcatag gactaaaaat ggtggctttg tcaaagggaa attcatgtta actcgtgcaa
    22141 cgaatcttgc aaatatgtct tatattcctc gaagatttaa acaaatggca ataaaaatcg
    22201 ttaagaaagg aaagtaacat gaaagatgaa atcattgctg caatcagtag caaattaaaa
    22261 gaaatctatc ctgatgggac aatatatctt gattcagtta tgcagtcaac taaagatttt
    22321 tatttcgttt tatcagtaat ggaatctgga actgaaaatg taggaattga tattcaaaat
    22381 gtttctttct taattgatat tgcattgatt gataataaac ctaatagaaa tttaattaat
    22441 gaattagtct cacgttgtgg gacttttttt aatacgatta caatcgatga gcaaatatta
    22501 tttccaaaag cctatttacc tgatgaagca gatggtgttc aacatattcg ttttacatta
    22561 gaatttccac aatatattga atggagtgaa agataaatgg gagaaaaaag aagtaaaacc
    22621 ggaattattt ctgtcgaaaa acctacttgg tttcctttgg aagatgagac agggaatttt
    22681 ccggtatatg gtactgcgtt gccaatggga acagcagtaa gtatcaaacc aacagctaat
    22741 tatgaaacaa cacaagatta tggggattca gtggttcaag atcaatttac agcttttggt
    22801 ggtgcagaag ttgaactgga agcaaatgga tatactcata aagttttatc tacaattact
    22861 ggagggaaaa ttgttaaagg tggtgccttg cgttctggag aagatattgc gcaagatggt
    22921 gcgtttgcat atagacgtaa aaaatcgaat gggaaatacc gttatacggt cttttataaa
    22981 ggacagtttg ctctagattc agatgaaaca tcaacgattg aaggaagtaa agtaagtttt
    23041 actcatccaa cttggaaggg ttcatttgta gatgtaccag gacttggata tatgtattca
    23101 gttgatgaag atgatgaagg cgtggataaa gcaatgatcg gaaattggtt tacaaaagta
    23161 gcaattccaa ttgaagaagc agaactttca ggaggtacag aataatgtca aaatatcaaa
    23221 caacgattaa attaacgaaa aaagatgaag aaggtaaata tgagcaagta caatttaaat
    23281 ctgctgaatt tttaccagga acagttgtag aagatgctgc aggagttatg gaagaaatgc
    23341 aaacagcgac tgataaacaa tcagttaaaa aagctttaag tcgtgcttat tcatttattg
    23401 cagatacttt atttgaggga caatttactg gtgaagatta ttgcaaagga attgatgctc
    23461 gtgagattgc ctcattgaca ggaaaattat tgaagtctgt tactgcaggt tttgatgaaa
    23521 cttatacaga gacgaaaaaa aagtaagtga ggcgctcaag tcaccttcat ttaagtattc
    23581 gattacttac cgagaattag atataaagac gcaattactc gaagcaggtt ggacgttacc
    23641 agaaattgaa cgtactgact tggatgagtt aatgcgtctt tttgctttta gagatgcggt
    23701 aaaagagcat gaagatgttg agtactatga taatttcact caattttagg aggtgatact
    23761 ttgaataacg atgacctaat tctgaagatg atactagatg aatctggttt tactgccggt
    23821 atgaataatg ctgtcaaaaa gttgaactct tttgatgaaa caatcgaaag aaaaagtaga
    23881 aatagtggca gttcattagg tagtatttgg aaaatatttg ctggaagttt tctagctagc
    23941 ggagtaacta gaatcgtagg agctggcttt gatctaatca agggttccat aagtggagcg
    24001 gttgatcgag tagatacgat gaataacgct ctacgaaatt tccaaaatat gggatttagt
    24061 aattcagaaa ttatgaagaa tatcggaaag aatgggcttt tatctcaagg tattcaagga
    24121 cttcctactg ctttgaatga tgcgataagt catgttcaac ttcttgcttc ttctacaggt
    24181 gatttaactc gttcaactca aatatttaaa gctttaaatg atggaattct tggtttcggt
    24241 ggttcaactg atcaagttaa tgaagctgtt attcaattat ctcaaagttt ctcaaacgga
    24301 aaagtagacg cacaaacttg gaactcaatg atcaacgctc aacttggacc tactctttct
    24361 gctattgcta aaaagatggg aattacaatg ggagatctga aagaaggttt gtctcaaggt
    24421 aagatttctg ttgaagaatt ccaaaatcaa ttaatagaaa tggataccaa aggtggcgga
    24481 ggacttaaat cattaagtca aatcgctaaa gattctacta agggaattaa aacctctata
    24541 caaaatgcaa aaacagctgt tacacgtggt gttggtgagg taatagaagg attaaataaa
    24601 gcattagtag attcagattt aggtggattt aagggaatta ttgataaagt gggcagttca
    24661 atggaatcgt tcttaaaagt aattgctgca aatattccta cagcagtatc atttttaagt
    24721 aatttattta acgaaattca aaaatttggt tctgctttga aattcatgat gccctttctt
    24781 gttcctgcag ctaccgcttt tggagcgttc atgtttcaac ttaaaggtat accagcaata
    24841 ataaaaagct tcaataattt taagaatgcc ataattggcg ttggaaattc attaaagatt
    24901 atgggggcaa tagctgctgc taattcattt gttttaatta ttggtgcagt cgttggtgca
    24961 atagctgttt tcggttactt tatggcaacc aatgaagagt ttagaaacaa ggttatatct
    25021 atttggaatg atgtgaaaga ttccgtaatt ggtgtattaa aaaatataaa ggactgggga
    25081 attgatactt ggaattctgc taaagaaatg gcatcaaatg cagttgaggg tgtcaaagat
    25141 gcttggtcag gaacaaagga atggttttct aacacctgga aaggtattaa ggatggggcc
    25201 acaggtttat ttgataaaac agtagagact tcaagaaatg cagtcgatag tgtaaaaaat
    25261 gcatggtcta atatgaagaa atggttttct gatacttggc aaagtataaa agattcagcc
    25321 agagaaaaat ggaatgaaat taaaggttct attatgtcgg ttgctggtcc attgattaca
    25381 ggtataaaga atgcattttt acatgttact ttttatctag agaccttatg gaataattta
    25441 gttgagattg gtaaaaacgt ctttgagatt ttaaaaaatg ttatacttgc gccggttttg
    25501 tttattacct ccatgatttc tggtggatgg gaagaaacaa aaaataatat gattgctgtt
    25561 tggaataata ttaaagaaag tgccataaat atttgggaat ctataaagaa tatatttgta
    25621 agttatttta caaatattta ttttgctgca ctcaatattt ggacagggtt caagcttaca
    25681 ttgataaata tttggaatga agtggtaaat caagctaaat cgatttggtt aaacataaag
    25741 tattttttta ttaatctttg gattgatata aaatattttg caattcagaa atggattgaa
    25801 ttgaagtttt ctattattca aacttggatc gatttaaaat ataatgccat tactacttgg
    25861 aataatatta aacagttttt caaagatact tggaaaaata ttaaagatac ggcatacaat
    25921 acgtggattt ctataaaaaa taccatgatt aatacatgga ataacattaa ggactctttc
    25981 tggaatattg ttactggaat tgttaattct gctgaaaatg catggacaaa tctaaaaaat
    26041 ggtgtttcaa atgctattaa tcgggtaaaa gaaatcttta attcgttaag ggaagtcaat
    26101 ttatttgaaa ttggcaagaa catcattgat ggacttataa atggtgtaaa agaaaagtgg
    26161 aatgctttga aagatactat caaaggtatt gcaggtaata tcaaaaattg gattaaagga
    26221 gctttagata ttcattctcc atcaagatgg atgcgtgata tggttggtaa aaatattgtt
    26281 caaggtatta tcgttggtat tgataaagag caaagtaagt tagatcaaac catgacaaat
    26341 ttagtaaaaa caccgtctgt tcaacctgta gtaaatggaa taagtcacct accaattaca
    26401 caaacaaagc aaaatatatc tgctagtgaa acaaaagaaa ttcatttaca tttaaatgtt
    26461 tatggagatt tacctgattc aatggttcgg aagattacca caaaaattaa aacagaattg
    26521 acaagacaaa tgaaacgaga tgctggtgca gtaggaggaa cattatatgc aacttaagag
    26581 aggacaattt tttattaatc aacattattc ttctgaattt aatgtgtata ttcaaaacag
    26641 gcctgcttct gtttcagcta gtcgagtaat tgaattgaga gagcgagaag gaaatgattc
    26701 tatcattatt gataaaggct attacagaaa tgtgactaga aagattgagt gttactataa
    26761 agcaccttca attgatgtgg tgcaggagtg ggaagatcga atcactgaat ggttagatat
    26821 gtcgtcttat agtgatttta ttctttacta tgatgaacaa tatatttatc aagctgtagt
    26881 aatagaagga cctgaattta aaggaacaag aaaaacagga aatattgtcc cttttgaatt
    26941 tacagtgagc attcgaccat ttaaggaaaa ttacaatggt agatttacta ttcgacaaac
    27001 agaaactttt gaaatttata acccagagaa gtattcttca aaaccgctta ttaaattgag
    27061 tggttctgga gatgcttctt tttatattaa taaggacaaa tatgatttga aatcgttgga
    27121 cagagaatta tttatagatt ctaaattaga agaggcttac cgaaaattag atggtaattt
    27181 agaacatcag gatcaagtca ctttattttt agattttcca tttttatatc ctggaaaaaa
    27241 tgaaattaaa tggacgaaaa atattcattc atttgaaata atgcctaggt ggtggagaaa
    27301 agtatgaaac caagaatata tagtcctact gaaacagatt tctcaacgaa tggcttagga
    27361 attttaaagg attgtacaag atgtgaaata tatgaagtag ctaatggaaa atatgaatta
    27421 gagttggatt atcctttagg aactagattt gatgaatatt ttgaaaatga ctatcaaata
    27481 aaagcaaagc caaacgatca agaagagtat catatctttt ttattgatga taaagatata
    27541 gatacttttt taaatacagt aactatttat gctcagagtc gtacaaatcg acttggaaga
    27601 cgggtagtca ctcttgcgga agtagactct aaaactggtc aagaagcgat atcaattatt
    27661 gaaaccaaaa tggataaaaa atctgacata cgactttatt ctgatattac ggccgtttct
    27721 agcacaatct ttgaagcgag aaatgtttta aattgtattg ctggtgaaca aggatcattg
    27781 cttcaatatt ggggtggaga aattaaacgt gaaccattta agctttcttt gttaaagcga
    27841 agaggtcgca ataatgttgg aacgattcgg tatggaaaag atatgtctgg tttaaaggtc
    27901 aaattagatt ggacaggtat aaaaacaaga cttattccat atgctgatcc tcaaagtgag
    27961 gtaggtacga ctaatcgaat ttatggttcg ccagtagata gtgcctatat taataactat
    28021 cctgatgtgt atacagagca tgttcagttt acagaagaac aaggagtaaa agacattaag
    28081 agcttaaata aaatagctaa aaattacttt aaaacgatca atcctggctg tgataagcct
    28141 aaagtttcta ttacggttga atttgataag ttgactgata gtgaagaagc gaaagaattt
    28201 gcgaagatta gaaattatgg tctgtttgat acatttaaaa tatatcataa aaaatataat
    28261 atttatcttg aatcaaaggt tagtggacta caatacgatt ctttatctga aaaaactttg
    28321 aaattagaag caggagacat tcaagttgct ttttatcagc aacaagctgt aactattcag
    28381 gataaattaa aagattatgc aacgaataat tatatgagtg attttaatga ttatgtttct
    28441 tcaatgatta caggtcaagg cagtgcagga ggatatgtaa gtttatggcc aaaagaaaaa
    28501 ccttccaaca tctttattat ggataatcca gatttagaaa aggcaagaga agtactaaga
    28561 atgaataaaa atgggattgc tttttctaag aaaggatggc gggggccttt tacttcagct
    28621 tggacattag atagtatatt taatgccaat tttatacaaa ctggattaat caaagcagat
    28681 atctttcaaa attcttttaa caagactgga gatgtattga aattagttaa tggactactc
    28741 caaatttgga acaacaaaaa gaaaataatg gagttaacta aaaaaggaat ggaattttgg
    28801 aattctaagg gttcaattgg aacgattgga acaactgatt ctgctggtaa tccttttcct
    28861 ggggcttcta ctccaacccc tattgaagat aattctttag ttattcgtac aaatggagac
    28921 ggcaaatata ttttgatttc tcctaaagta ggaaaaggat tagttttatt aggaaatggt
    28981 aaagcaattt attttggaga cttagatgta caaggaaaac ttacagttaa aggaaaagaa
    29041 ataattcctg gacaaggtgg tggtccatcg ggaggtggag aatctactgg tgggtatcct
    29101 agtgaattaa aaacagatgc agaaaagaga gcttggagaa t
//
LOCUS       DAFEOM010000026        28684 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.027, whole genome shotgun sequence.
ACCESSION   DAFEOM010000026
VERSION     DAFEOM010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.027"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(23..1189)
                     /locus_tag="LP524_001378"
     CDS             complement(23..1189)
                     /locus_tag="LP524_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL2234441.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(1201..1554)
                     /locus_tag="LP524_001379"
     CDS             complement(1201..1554)
                     /locus_tag="LP524_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBL2234442.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            1682..2704
                     /locus_tag="LP524_001380"
     CDS             1682..2704
                     /locus_tag="LP524_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HBL2234443.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            2723..2884
                     /locus_tag="LP524_001381"
     CDS             2723..2884
                     /locus_tag="LP524_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234444.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(3143..3577)
                     /locus_tag="LP524_001382"
     CDS             complement(3143..3577)
                     /locus_tag="LP524_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234445.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(3687..5546)
                     /locus_tag="LP524_001383"
     CDS             complement(3687..5546)
                     /locus_tag="LP524_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBL2234446.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(5664..6823)
                     /locus_tag="LP524_001384"
                     /pseudo
     CDS             complement(5664..6823)
                     /locus_tag="LP524_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(7130..8554)
                     /gene="araA"
                     /locus_tag="LP524_001385"
     CDS             complement(7130..8554)
                     /gene="araA"
                     /locus_tag="LP524_001385"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBL2234447.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(8570..9268)
                     /locus_tag="LP524_001386"
     CDS             complement(8570..9268)
                     /locus_tag="LP524_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBL2234448.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(9225..10886)
                     /locus_tag="LP524_001387"
     CDS             complement(9225..10886)
                     /locus_tag="LP524_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HBL2234449.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            11104..12504
                     /locus_tag="LP524_001388"
     CDS             11104..12504
                     /locus_tag="LP524_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HBL2234450.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(12756..13835)
                     /locus_tag="LP524_001389"
     CDS             complement(12756..13835)
                     /locus_tag="LP524_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL2234451.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(13921..14880)
                     /locus_tag="LP524_001390"
     CDS             complement(13921..14880)
                     /locus_tag="LP524_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBL2234452.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(14892..16403)
                     /locus_tag="LP524_001391"
     CDS             complement(14892..16403)
                     /locus_tag="LP524_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBL2234453.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(16425..16988)
                     /locus_tag="LP524_001392"
     CDS             complement(16425..16988)
                     /locus_tag="LP524_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBL2234454.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(16994..17857)
                     /locus_tag="LP524_001393"
     CDS             complement(16994..17857)
                     /locus_tag="LP524_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL2234455.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(17871..18767)
                     /locus_tag="LP524_001394"
     CDS             complement(17871..18767)
                     /locus_tag="LP524_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL2234456.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(18942..20231)
                     /locus_tag="LP524_001395"
     CDS             complement(18942..20231)
                     /locus_tag="LP524_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL2234457.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(20203..21720)
                     /locus_tag="LP524_001396"
     CDS             complement(20203..21720)
                     /locus_tag="LP524_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBL2234458.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            21918..22808
                     /locus_tag="LP524_001397"
     CDS             21918..22808
                     /locus_tag="LP524_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HBL2234459.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(22881..23705)
                     /gene="nadE"
                     /locus_tag="LP524_001398"
     CDS             complement(22881..23705)
                     /gene="nadE"
                     /locus_tag="LP524_001398"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBL2234460.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(23717..25189)
                     /locus_tag="LP524_001399"
     CDS             complement(23717..25189)
                     /locus_tag="LP524_001399"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBL2234461.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(25826..26188)
                     /locus_tag="LP524_001400"
     CDS             complement(25826..26188)
                     /locus_tag="LP524_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBL2234462.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            26411..27163
                     /locus_tag="LP524_001401"
     CDS             26411..27163
                     /locus_tag="LP524_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL2234463.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(27303..28226)
                     /locus_tag="LP524_001402"
     CDS             complement(27303..28226)
                     /locus_tag="LP524_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL2234464.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(28241..28513)
                     /locus_tag="LP524_001403"
     CDS             complement(28241..28513)
                     /locus_tag="LP524_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234465.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         8478 a         5987 c         4883 g         9336 t
ORIGIN      
        1 ttttcccatc tgtctctttt tgttattctt tctgcttttt caacggattt tcttcataaa
       61 gagaagagat tgttgtgtac cattcaaaaa catgagtaat cactacttta gctgctgcat
      121 aaacaggaat ccccaagatc acaccgatga ttccgaacaa tttcccagaa gtcagcagaa
      181 ctaataaaat cgttactgga tggattgcta actgattccc tagtactaat ggagagatca
      241 agcgtccttc tattgtctgc tctactgcaa aaacgatcag tactttgatg atcatgaatg
      301 gacctgctac aatggctaaa aagatagccg gaatcatcgc taaaaatgat cctaagtaag
      361 ggatcaggtt taaaaaaccc gcaataatcc ccaacgtgac agcatactct aaaccaatga
      421 ccgcaaaacc aatcataaac atgattgcca cagcaaaagc cacagttagt tgtcctctta
      481 tataagaaga aacttgacta ttcatttcac tcagcacatt caacgtcggt ttttgcatgc
      541 gtgtcggtaa aaatttgaca aaataaggtg ctaattgttt accgtctttc aatagataaa
      601 ataagataaa cggcatcgtc agcagagcca ctaaaatagt cgctaccgcg ccgacaaaac
      661 ttccaagact ctgaactgta ctcttagata tatcttggat catatcgcct gccgaattca
      721 tgaacttatc acctgctgtt tccaactgtt cgcgaaattg gttgaacagc gggtcccgca
      781 aaatctcggt taatttatta tcaatcgtat ctacatattg agggaagtta ctgatgaagc
      841 tcactgtttg ttcctgaatc ttagggatga tcactacgct cccccatgcg attagtgcaa
      901 caaccaaaac aaacagccca atgatactgt aaagccttgg tacttttcgc ttttcaaagt
      961 aatcaactac cggattcatc aagtaataca agattcctgc taaaatgata ggcagaccta
     1021 cgattgctaa aaattgccag attggtgcaa acaaatatga aacttttgta aataaaaaga
     1081 cgatcagcaa tattaacaat acaacaagta aggctgtgac tacttgatta tttaaaaacc
     1141 agcgccaaaa ccacgatacc ggtctgttct tcttattttt tccttccata attcctcctg
     1201 ttagttataa aaaatttggc ttcccaaacc aatagcaggc agtctatgcg gatgaagata
     1261 gattccattt gcaatttgtc cttcttctgg acaagcatca aaaatcaatg atacgtgagc
     1321 tactgtcttt aaattcccat tggccatcgg accgacataa tcgatcgtat atacctgatc
     1381 atcaaaagca attgttccgc ctgctttcaa agaaaaaggt tcgtcttttg atacttcttg
     1441 aatcaccgaa tattttcgta aggcagctgt tgctgtttta tcgaacaaaa tcagcatggg
     1501 ttcttccttg tttattgctt ctgtcccaat ttctgtaatc tttgcctgaa tcattgcact
     1561 ctctccttta attgctttca aaaacagtat aacacattct cattctttag ccgtgtggaa
     1621 taattatgtg atagaataaa aacgaaaact atttcattac aattaggagg aatcagctca
     1681 tatgatcgaa aagataattt taggaaccta tacgcgtcgc gaaagtaaag gaatctatac
     1741 gatcgacctt gatacagaaa aagaagaatt agccaacctt tcattagctg ttgaagaaaa
     1801 tagccctacc tatgtagcaa aaagtaaagc tggcaatctt tacacagtta ctagtgtgga
     1861 tggattaggt ggtgccggtg cgtacgataa ggattttcat tttttgaatg cggtaacaga
     1921 gtctggcgct cctttgtgtt atgtagcggt tgatgaagca cgtcaattac tatacggggc
     1981 gaactatcat aaaggtgaag taaatgtcta ccatatatta ggagatggcg aactgaaagc
     2041 agcagattca atattccatc aagaagcgac aggtcctcat aaaaaccaag atcatgcgca
     2101 tgtccactat acggatctga ctcctgacca acgattagtc gtatgtgatc ttggtacaga
     2161 tcgtgtgtat acttatgatg tgtccgaaaa cggcaaattg aatcttgtaa ctaccttcac
     2221 tgcagagcca ggaacaggcc ctcgccatct tgtctttcat ccaaatggac aattcgctta
     2281 tttatttggt gaattagaca gcacagtccg cgtgctttct tataataaag aaacaggtgc
     2341 ttttgaagaa ttacagaaaa tatcgacctt gccagaagaa ttcgacggtg aaaatggagg
     2401 ggcagctatc cgcatttcaa atgatggcaa gttcctttat gcttctaacc gaggacataa
     2461 ctcgattgct gtattcgcag tttttgaaga tggttcccac attcaaaaca agcaaatcat
     2521 ctcaacagaa ggggattttc cccgcgattt cgcactagat ccaagcaata attttgtcgt
     2581 gtgtgccaat cagaacaccg ataatctgac attatatcgc cgtcatcctg aaacaggatt
     2641 actggaatta ctgaaaaaag atgtctatgt accagaatgt gtctgtgttt attttgatcg
     2701 ttagtaagaa aaggagaatg aaatgaatca gcaagacgta ttagacagac tacgggaaga
     2761 actaaatatt cctttctttg atgggaaaat cgaagataaa gagtatagcg aagaggaata
     2821 tcagaaactc aaagccgatt tacagaacta ctttgaacaa tatgtacaga atgtagaaaa
     2881 ctaatcagct ttccatacta tctgaaaaga acatcaggac aagacttttc cgtctatcct
     2941 gatgttcttt tctacaaata aaaaaccgcc gagatggtta gactcgacgg aaaaggagta
     3001 tttactcgat tttgagtaag tacaaggttg ttgttggtta taggtatgtc attatcataa
     3061 aggattttga tcactctatc ataaaggtaa tattacattt atattagata actctagttt
     3121 ttattgttat caaagatttc ttttattttt tcaacaacaa attagtcatt ttttggatgg
     3181 cagctgtcaa ttggtctgtc gcttggtctt gttttgcttc agatgtattg aatagatctt
     3241 catctacccc tgattcaaga tcgatacttt ggatcatttc ttcacagcct gctacatatg
     3301 aaagataagc tttttcaaaa tttttatgaa tgcccattgc ttttgctggt ggacgcaaag
     3361 tactgacttt cataagcatt gcccgatatt tttttgttcc ttcttcaaat aacaggatga
     3421 tctcttcaag agtcgatttg tccagttcac ttgtttttcc ttcatcaatt gcgtttctga
     3481 tcacttcata tttcggatgc attccttctc cgattgcttc tgtttccttg acgatttgat
     3541 cgatggactg cacataatag ccaatattcg gtaacatgtt cgttttccta ctttctccat
     3601 tgttgttatt atttattcta cttgttttta tcctcaaatg acaagtactt gaaaaaattt
     3661 aatggtttct atacagctcg ccattcttat tcgtgctcaa agaaaccatc tgctaaacta
     3721 tattgtttcc ctgcgacaat gtcatattgg atcaattgat attcgtcgta aatctcttgt
     3781 tgcgagcgat tgaattgcgc ttctttatac cactgtccgt tttgatagta cagttctcgt
     3841 tcaaacgccg gaagttcctg ttctaatgat aaaagcagct gataaaagcc atttgttgtc
     3901 tgattcgtct gttccattag attcgctgca aaataaaacg gactggtgat tgcatcgtga
     3961 gtcgtttgct tttctaattc tcctttactg tcgaacataa gaaattgtgt ttcatgcaat
     4021 gtttgtttgt cattttttgc ttggatcgta tctgaataga tccctggaag atgatccccc
     4081 caaaaaacaa ctaaagtcct tctagaaaga ttcttcaatt cttccgtgaa ctgcttcagt
     4141 gccgtgctgc tataggaaat atcttgcaga taattctcta agtcaagtgt cccacttcca
     4201 tctgaagttt ttgcaggata gtctagatta tcatactttc catcgtaggg catatgtgtc
     4261 tgcatcgtga ctaaatgaat gaactgaggt gttttttctt ccttgagaag tgtcattact
     4321 tctttgtaag ctgactcatc agaaatatat ggattattct ctatcgtatc cgtataggtc
     4381 atcgtacgct cgctgatgaa ctggtcaaat cctaatgtct gataaacatc ctctctttta
     4441 tacatcgaag tattgtatgg atggatcgct gtcgtctggt atgattgtgc gtttaacgct
     4501 gaaacgatag aaggaagctg atccattttt ggtacaagca tcgtgtaagg ggtggtgagt
     4561 tgcgcattaa ataacgccat agaaaaacca gtcaatgctt caaattcgat atttgctgtt
     4621 ccaccgccat agttttgaga aagcatattg ccactatatg tttgatcagc tacttcataa
     4681 taatctgcca atggctcacc agatacttct acgccgttta atcgactagg atctgagaaa
     4741 ctttcactca tcacgaacac gatgttcggc gactcttcct ctacagtttt atttttttca
     4801 tcggctagct tctggtattt ttcagtgatt tctttgatct tttctttcga atatccttca
     4861 ggttcatcca ttggttctac cttcagattg tacaaaaacc caccgataaa accagtgtta
     4921 tagtagttca ttttctggct gtaaggtatc catagtgccg ttttattata tgcttttcgc
     4981 aacaaattat cgggattgtt gaaattactg atatacccta gcagtccaat tgccacaagc
     5041 agtgtcgtta atcggatcaa ttggaatcgc cttccttttt tcaggcttcg aaacatgtac
     5101 cagcaaaaca atcctatcac ggttcctgcg gcagctaaga tcactgtaaa cagcatagtg
     5161 ccggtcatat ctttcagcaa tccgatttca gtgatcatct tcaaatcgtc tggataaatc
     5221 ggttcttgcc gataactcat tttcatataa tttgcaaaac ctaatacacc gatggctacg
     5281 acatacgtaa gggaacctaa agggacagat cctgctagtg caatcagaaa aataagaata
     5341 atcagcaaga cgagacaggc taagaagaat ttttccgtat gccaagaaaa agcaaatttc
     5401 aacgcaaggt ctacggatag atcattttga caccactgca aaaataaatt actaaaaaca
     5461 acaagaaaaa agatactgat gattgctgct gttcctttta aaatcttttt ctgtttggtc
     5521 gaaagtctgc ttatatatct tttcaaatgc ttgctccttc atttctatca ataaaagaat
     5581 gttgcttctc ttattttagt tgtacgaaat tcgaaagtaa agaacggaaa gaagaattaa
     5641 tgaaatccaa atattctctt tctttatggc ctttctggtt tctttaataa gcggaccttt
     5701 cgtactccag aaattttttc tagctgttgt ttcacttgat tgagctgttc aaaatcagta
     5761 ctgtctatgt caatcaatat gtagacatgc tcattcttat ggttacgcga tatatctgcg
     5821 atcgctagct cgttctcatt cagcagagaa aatatctctg ctaatatatt ggttttcttt
     5881 tgataaaata tagtgaatcg aaacggcgct tggaaaaaca ttcgtgcgga tggataattg
     5941 accgcttctc taaccgtacc gaacaaaaga taatctttta aggcttcaac tgccaaacgt
     6001 ccgctgtctg ccaaagcttc ctgagtcgat cccctagatg aggcaacata agtatacggt
     6061 cattttctaa gaactcttct tctggaaaat cagtcaagta tttagccaga tgttgttttt
     6121 ccaatgcttg cataacagca tatttatcaa caatttcact tcttcctaca ttgataagta
     6181 cagcatcttt tttcattttt tctatattcg cctgatcgat caatcctttt gtatcttctg
     6241 tcaacggcag catgactacg atgtaatccg aggtagaaag aactgtttct aaatctgctt
     6301 gccgaacata atccaactgg gcatcgcgga tagaataacc caagacatcc atccctaagc
     6361 tatagcaact tagcgctact tttgtgccta tcgctccaag accaagaaga ccgatggttt
     6421 tgccttgcaa ttctcgtccc acataagcac ttcttttgtt ttctgcctgt tctaaaatat
     6481 taggacctgt tagtgtttgt accatacgac ttgcttcgat tatcggacgg gaagataaga
     6541 gcaaacagca caaaacaagc tcttttactg cattagcatt tacacctggt gtattcatga
     6601 cgatcgtccc gttttcagaa gctttttcaa cgttgatcgt gttcacccca acaccagctc
     6661 ttgaaattgc tagcaaatct ggtgtaagcc attcatcttt cactttcgta ctacgaataa
     6721 ctattccttc ggctggcaaa tcatctatta ttttgaaata ttccccttgt aacttttcta
     6781 tctcttgttc tggaatagca tcaatcaaat gaacgtgata cataaaaccc cctccttact
     6841 aacaagtatc tcgaaatttc tgcaattaga caactgttta ccatttgact tcctagaagc
     6901 aaaaagagca gttagagcag agtaggctga gtgataaaaa taaggctatg acaagttact
     6961 gccataagcc ttattttcga gagcaaaata ttgagggtga gatatcttga taaatggtgt
     7021 tccgttagct gacattgata ataagactaa atttcaaaaa agttgaaaag caaatttcgt
     7081 caatctcttc ttatttctta aaactaagtg gctttttcac aacctctttt tatctaataa
     7141 ccactgtttc caactcaact aattttgccc acgtttcaat ttgatctgtt gttagattta
     7201 gcgagacaac ggtatgatga ccgccacctg cctgaatcca tgaatggaca ccttcatgga
     7261 aattcggttt agttttccat aacacacgtg caacaggtaa attaggtgct tcagtcgttg
     7321 gttcaaaggc ttctacttca ttgatcagca gtttataatg cgtaccgaaa tctgccatcg
     7381 atacgactac cccttctcct gctttcccat caaatacaag acgcgccgga tcttctctat
     7441 ctcccattga taaaggagaa acaacgattc ttggtttcgt cgcagcaagc gtcggatcta
     7501 cttccatcat atgggattcc aagattgctt cttggccact agctagctcg taagtataat
     7561 cttccatgaa tcctgtatct ttccctcgag ccatgatttt cagcaaccga tcaatcgctg
     7621 cagttttcca gtcgccttcc ccagcaaaac cgtatccttc tgccatcaaa cgttgtacag
     7681 ctagtccagg tagttgtttc attccataca ggtcttcaaa attagtcgta aaagcgttat
     7741 agcctccttc ttctaagaaa cggcgaatac cgatttcctg ctgagcttgt acttttacat
     7801 gttcctccca tgtcttttct tcataatcac catagtcgaa ggtatacaat tccttgtact
     7861 cttcaaataa ttgatcgact tcttcgtctg atacgcgatc gattacttgt acaagatctc
     7921 cgataccaaa ataatcaaca gtccaaccaa attgaatctg tgcttccact ttgtctcctt
     7981 ctgtgacacc cacgtttctc atgttgtcac caaaacgagc cacttttatt ccaaaacttt
     8041 cattataggc tactgcaaca tccatccaat cagcaatctc tttttggatc tcaggtcgtt
     8101 tccagtagcc aacgacgatt ttattttgtt tatttaatcg agcattgatg aacccatatt
     8161 cgcggtcacc atgagcggat tgatttaaat tcatgaaatc catatcgatc gttttccaag
     8221 gaatgctttc gttatattgc gtagctaaat gaagcaatgg tttttgtaga agctttgtcc
     8281 cgcggatcca catttttgct ggtgagaaag tatgcatcca agtgatcaca cctgctactt
     8341 cttctcgata attgacctct ttcatgattt ttgtgatcgt atcagcagta actgctaatt
     8401 cttgaaggcg gattggataa ggcaattggc cattttcatt cagttcatcg accatttctt
     8461 gtgcatgttt ctttacttct cgcagtgctt cttccccata taaatgctgt gatcctacaa
     8521 caaaccaaaa ttctttttct ccaatattca acataattag ccactccttt tatttgtttt
     8581 gtccgtaata cgcatttttt ccgtgttttc gtaaataatg tttatctaaa atccgttgag
     8641 gcagctcttc tgaaaatgaa ttgagctgtc tggtaaataa attcatcttg catacttcat
     8701 ctagtacgac tgcgttcatt accgcacttt gcgcatcttt cccccaagta aatggaccgt
     8761 gtccgtgtaa aagtacccct ggaattgcta agggatcaat tttgcgttct ttgaatgttt
     8821 ctatgataac ttttcctgtt tcatattcgt acccgctgtc gatttcctgt tgtgtcaaaa
     8881 atcgagcaca aggtaccgat ccataaaaag tatctgcatg cgtcgtcccc atcgcaggaa
     8941 catccaatcc tgcttgtgcc cagatcgtcg cccatgtcga gtgagtatgg acgattcctc
     9001 cgatttgagg aaatgaacga tataaaacag catgagtagg cgtatccgaa gagggattga
     9061 gtttaccttc aatgatatga ttgtccaagt ccacaacgac catgtcgtct gctgtcatct
     9121 cttcataact gactccgcta ggtttgataa cgaaatatcg tgtttcagga tcaaaagcgc
     9181 tcacatttcc ccatgtatat ttgacgagtc cgtgtttagg caagtcaaga tttgcttggt
     9241 atacttcttc tttcagttgt tccagcattt tctatcactc ctttttcttg caattgatga
     9301 atcgctgttt tttcaatgtc gatcccctca cggtaacgtc tcataaatat ctgatagcct
     9361 gctatttctt ctgctgaagg tgtgatggtg attccttggt cttgtccaaa cacgtcttct
     9421 gccaagaagt tttctaaaga cttcccatgt cgatccatca tataggctgc taataacgcg
     9481 attccccacg ctccaccttc tccggctgtg tccatcactg taactggtgc ctccatagca
     9541 gatgctagaa tccgctgtcc aacttctggt gttttaaaaa tccccccatg ccccactaaa
     9601 cgatcaattt ggacattctc agatttcaat atatccattc cgattctcat tgcaccaaaa
     9661 gcactggcta aatggatccg catgaaattc gctaaatcaa aacggctacc tggtgtacgt
     9721 acaaataatg gccttccttc atttactcct gtaatgtttt caccggaaaa ataaccataa
     9781 gaaagtaatc ccccgcaatc cgtatcgcct tctagcgctt tgttgaataa taccgtgaat
     9841 aatttatctc tgcttatctc catacccaaa gaattggtaa attcttcaaa aatactgacc
     9901 caagcattga tatccgaaga gcagttattc gtatgaacca tgcctactag ttctcctgaa
     9961 ggtgtagtga ccatgtcgat ttccggatgg acattttcta gttctttttc taatacgatc
    10021 atagcgaagg cggaagtacc agccgaaatg ttccctgtcc ctttggctac actgttcgta
    10081 gcgaccatac cggttcctgc atccccttct ggcggacaaa ccgggattcc tggtctcaaa
    10141 tttcccgtag ggtcgatcag ataagcccct gattcgctta acttgccagc ttcttctcct
    10201 gctactagaa ctttaggcaa aatgtgatcc aagtcaatcg ctacgtgcgc tttttcgatt
    10261 aactgaccaa aaatattttt catttcttga tggtaggttt tcgttgaagg atcgatagga
    10321 aacatgcctg atgcatctcc tacgcctagt actttctcac cagtaagctg ccaatggaca
    10381 taacctgcaa gcgttgtcaa aaaattaatt tctttcacat gttcttcttt attcaaaata
    10441 gattgataca aatgagcgat actccatctc tgcggaatat tgtattgaaa tttttccgtt
    10501 aatattcgtt ctgcttcttc agtgattgta tttcgccaag tacgaaatgg tactagtaac
    10561 tgttccttgc tatcaaatgc tagataaccg tgcatcattg cactgaaccc aatcgacccg
    10621 attgtcgtaa gattctcgcc atagatttcg aatacttctg ctgccatttt tcggtaactg
    10681 acgcgcagtc ctttccaaat atcttctaat gaataggtcc agataccatt ttctagtttg
    10741 ttttcccact cataactacc agcggcaata ggagtaaaag atgaatcaat caatacagct
    10801 ttgatcctag ttgatccaaa ctcaatccct aaagatgtac gaccatctat gacatctaac
    10861 atactttgcg catttacttc cttcataatg atttaactcc ctccttctcg tcaaacctga
    10921 tcaaaccgtc tttttagtag cgcttacaat gattattgta tacttttgta cgtacatgtc
    10981 aagcagttat ttacaagaat acaataaaaa acacttgttt tgtacgtaca taagttataa
    11041 aatgaaattg cttacaaaat ctgatcaacc gccttctcga aattctagaa ggagagaata
    11101 cttatgaaaa aagaaaaaaa agtttcctca tcgtttattt atttttttgg cgcatttggc
    11161 ggcattctat ttggatatga tattggtgtt atgaccggcg ccttaccttt tttacagcat
    11221 gattggggtt tggcaggtaa agcttctttg ataggatgga tcacgtcttc ggtcatgtta
    11281 ggtgcgatcc ttggcggctc tttatctggt cttctttctg ataagcttgg acgacgaaaa
    11341 atgattcttc tttctgcact tatttttatg gctggttctg tcctttctgc atctgcccct
    11401 cacaatggtt cttattttct gattgcagca aggattcttc taggtttagc tgttggcgct
    11461 gcatctgccc tagttcctgc ttatatgtct gaaatggctc ctgctagact aaggggaaga
    11521 ttgtcaggga tcaatcaagt gatgattgct tctggcatgc tcctttccta tgttgcagat
    11581 tatttgctaa aagggttgcc tgaaacgatg gcttggcgcg tgatgttagg gctcgctgct
    11641 gtccccgcgc tgattttatt tttcggtgtt ttagctctac cagagtcacc gcgttttctt
    11701 atgcaatctg gccgcttgga agaggcaaaa agagtattga actatattcg gacaccaaaa
    11761 gaagctgaac aggaattcga acaaatccag ctgaatgtca aacaagaaaa gacaaccgga
    11821 acttcttggc atactttgtt tttagaaaaa tatcgttcct tagtcattgc cggtattggt
    11881 gtagcagtat tccagcaatt tcaaggagct aatgctattt tttactatat tccgctgatc
    11941 gtagaaaaag cgactgggca tgcagcgagc gatgcattga tgtggccgat catccaagga
    12001 atcattttag ttgctggttc tttgctcttt ttggttattg cagacaagtt caatcggcga
    12061 actttattga aaataggcgg tagcgtgatg ggactttctt tcatcctacc agctgtttta
    12121 ggaactgtac tggatgctca tacaaattcg ttgttgattc tcttgttttt atgtatttat
    12181 gtcgcatttt attcttgcac atgggctcca ctgacatggg taatcgtagg cgaaatattc
    12241 ccactagctg tccgcgggcg agcatctggc ctagcctctt cttttaattg gattggttct
    12301 ttcctagttg gcttgctttt tcctgtgatg actgcttcta tgtctcaaga aatcgttttc
    12361 ggcatttttg ggatcatctg ctttttaggg gttttattta ttcaagagat cgtaccagaa
    12421 actcgtggga aatctttaga ggaaatcgaa caaagtgcat ctaaaaaaac ttatcctaaa
    12481 cgtatttcta tccatcattc ttaaagaggt tgtgacagaa gtggtcagct tcaagaaata
    12541 agaaggaacc gcttagtttc cagtcacaag gaacaatttc aaaaaacagc ttctcatgtt
    12601 tgatgaatat tgtgacttgt gaccagttgg taggagaaca ccgtttagtg gagaggtggt
    12661 gaaagaaacg ttcagccttg agcgatgaca aaagctctca caaacattgc gacgagtaac
    12721 cgtaggagca ataagaggcg gtaaaaaccc gtctcttatt tttcgttttc tttcactgtt
    12781 tcccgttcaa ctagtacagg ttcatagaga atatttggct gtgcctgtcc tttttcaatc
    12841 gttgtgacga tccaccgtgc ggcatcttgt cccaactttt cttttggatg cgtcaatgtt
    12901 gtcaattttt tattccctaa atggcttaaa gaagaatcgt ctgttcctac gatcgatagg
    12961 tcatctggaa tatgatagcc attttctgtc aacttatcta gcattaaact agctatttga
    13021 tcgttatagc aaatgatgcc atctaaagct tgttcttctt tcaaccgttg tactgttttt
    13081 tcagcgattg tttctcgtgt ttccgtggtg aaagtaatga tatcttccgt caaaaaacgc
    13141 acattatact gttcacacgc gctgataaaa cctttcatcc tgtattttcc ttgaagatcg
    13201 tcgatttttg tgatgaacaa cagacgctga tgtccttttt ttatcaaata ttctgctgcc
    13261 ataaaaccga ctttcacatc atctaaacaa atataagagg catttaactc ttcataagca
    13321 gcattgatca tcaccatcgg aatgccttgt tctctcaaag caacataaag tgctaagttt
    13381 gggttatact gattactttt cgtaggctca acgatcaaac catctacccc ataagcaatc
    13441 attttcttca aacactcttt ctccaattcg tgatcgttat tcgtactggc taaaagcaat
    13501 gaatacccag attctcgtaa ttcattttcg attcctcgga taatcgacgg aaaaatataa
    13561 tcagagagat atgtggtgat gacaccgatc gtttttgttc tgttcaatgg tgttttttct
    13621 ttcttatagc tgtcatctac atatgtgccc gacccttttt ctttacgcag atagccctca
    13681 tttaccaaca cagcgattgc ttgccgaatc gtatgtctgc tgacgtcata tatttcttgc
    13741 agcttgaatt ctgagggaat ggttgtattt acttcgtatt cctcacgaag gatcttttgt
    13801 tttatctctt ctgcaatatc ttgatatttt gccatttgcc ttgccccttt ttatttgtac
    13861 gtacatatat cataacgagt ctgcaaggtt tattcaagca aaaatcaagg tttcttgtgc
    13921 tcataaggca tacggaacag gttcaccaaa ttctggaaat ccttgctcat tccacgtaaa
    13981 gcattgcgcg ttcgcgtgcc gattaggatt gtctaacgga tccccttttt ggttcttctc
    14041 tggtcgagca tgatagatca aaatatcaaa gcacccgtct tctgaaaccg taaacgagtt
    14101 gtgaccagga ccaaattgct tatttttttc agaagaaacg aatactggct ccgataattt
    14161 atgccatgat tcttttaata aaagattctt gtgctcatca gcccacagca ggcccatggc
    14221 atagttttca tctgttgcac taccagaata cgtaatgaat attttcccat tgcgagtaag
    14281 gactgctgga ccttcattta ctgaaaaacc ccgtttttcc cattcatact caggtataga
    14341 aaggaggatc tgcttcccct ttaacgtcca aggattatcc atttcggata gataaagatt
    14401 agaattccca gtaatttcag gatcttgttg ggcccacaca taatatagct ttccttgaga
    14461 agagaaagtt gttccgtcta aactgaaagt ttcccttttc gtttttattt gtcccttttc
    14521 tatccaatct gtattcattg gatctatatg ctgattttca agtacgaaca tgcggtgatg
    14581 atgagattca tcacgtatag ttttatgatt gcttgcagca aagtaaatat accattttcc
    14641 attgatataa tggacttcag gtgcccagat caattcactc atcggtcctt cttcatgagc
    14701 ctgccataca atcaggcttg cagcatgttc taattcattc agtgactttg cccttcttaa
    14761 ttcaatcact tggtagcctg gaaccgatcc tgtaaaataa taataaccat ctgtatgttt
    14821 atatacccaa ggatctgccc gttgccgaac gataggatta ttataaagtg tcttcatcat
    14881 aacaaatttc ctcacatttt ctttattcta attacattcc aagagtattt ttctatccct
    14941 acatttaatt cgtcatgttt ttctattgaa aaatagttta tttcaggttt tattttttct
    15001 ggttcttcac gtgtattttt gtccaacaga tcgctactgg ttaatgtgta gtgctctcct
    15061 tgatctagca aattgaaatc atgttcaatc gttatctttt gttgttcatt tgtgcgattg
    15121 atcaagaaaa tagtcagctg attgccgtta tctacgatca cactgtccac ataaggaaca
    15181 ttttttatct tttcacaatc ataaacaggg caaatgattt gttgtttcaa tacttgacct
    15241 ttagcatatt tcgataagtg ttgcatcaca taataagttg gattcctcca tgcttgtcct
    15301 ttagcatctg tcagtattaa aggtattgtg tttactaaca atgattgaca actaatcttt
    15361 acgcgatctg cgtgtcgtaa aatagccagc cctaatgaac caaataataa tgtatcttcc
    15421 atagtaaagt aacaccaatc gatgggacta ccgacttcaa aatccctatg tcttttttcc
    15481 ggttgggcat gtacattcca ttcatcaaac gctagataca ttgttttctc acttctcaaa
    15541 tatcctttta cataatcaca agtagcaacg atttcttcaa tttgacgatc gaatcgataa
    15601 gcttttgcaa ggtatgtcgc tgtatcatca cgctcatttt taggaggttt agtcaagtct
    15661 tcttcatcat acttatcaat atagttatgc aaagctaaat aatctacatt ctcataagct
    15721 tcttctaatg cgatacgatc ccattcaggg tacgatggta aacgtggcgt agagcttcca
    15781 actaagattg tttcaattga atcatccact aacttcatga cttttgaggc ttcatttgct
    15841 aacctaccat attcataagc actcttagcc cccacttgcc atggaccgtc tagctcattc
    15901 cctaaacacc ataatttaac gccaaaaggc tttttttgcc cattttttat tcttaaatca
    15961 ctccagaagg ttccttttgg aaaattacaa tattctacta aattttttgc ttcatcaatt
    16021 ccttttgttc ctaaattcac cgtgaatatc ggtttcgctt gtacatgact gatccattta
    16081 aaaaattcgt ggagtccaaa agaatttggt tctatacctc gccatgctaa atcaattttt
    16141 tttggtcgtt gttctttcct acctacagta tccacccaat cataaccaga tgtaaagttt
    16201 cctccaggat agcgaatgac acctaattct aattccttta cataagagag gacatcttgt
    16261 ctaaacccgt caccatcagc tgtttcatgg cccggttcat aaatccctgt atagacgaca
    16321 cttcccatat gctcaacaaa tgagccaaac agccgagaac tgatctctcc taccgtaaaa
    16381 tcctctctga tttttacttt cacttcttcg tccaacagct ttccctacct ttcatttcct
    16441 atagtcaaga taatagatac aagtttccat acgccaccag caaagataaa aataaaagga
    16501 gggaaaagaa atatgagcag tacaaatcct aataacaata cgcaacaaag aatatttctc
    16561 aaaaaatcaa aaaatacttt ccatgcactt tgttcaaaaa tttgtaaaaa tgtgatatct
    16621 ttgttttcag aaatcacata tatccaggat aaaagaaaca tggaataaat aagtaaaact
    16681 acatatccac taattgaaag tacgattgtc tgttcttcta ataattgatt aacaagaaaa
    16741 taaataatga tcgttaacag actaaggaac aagttcttac caaaatgttg ctttattcct
    16801 tgaaagaaaa aataaatgat atttcctgat ccctcacctt tccattgcgc cattacataa
    16861 aaaacagcac atgtagcagg cacgatagta ataacaggca aacacacgat tacccagaga
    16921 atacctaata agatgtaatc agaaaccaca atcaactgtc cataaagttt agtctcaact
    16981 attttcatct tcctcactct ttcattccag acatagctgt tccttgaaca aaatatttct
    17041 gtgcaaaaat ataaaccact agtaatggca gcattgaaat aaccgttcca gccattacag
    17101 gtccataaaa agaaatgttc tgccctgtaa ataaagctaa ccctgctggt aaagttctca
    17161 tttttgaact acttgtcaat atcaatggat aaagcaagtc attccaacta ttcatcagcg
    17221 tgaaaatccc taatgataaa agagtaggtt tgctcaatgg caacatgatt ttcaaaaaaa
    17281 tattaaactc acttaatcca tcaatgcgtg ctgcctcttc caaagaatct ggtaaagtaa
    17341 taaaaaagga acgcatcatg aagataccaa atgcagttgt catacgagga atgatcaaac
    17401 ctgcataagt atcaagcatg ccaagcaaat tgacttcaat aaatagagga atcatgaaga
    17461 cttggaatgg gatcatcatt gtcagcaaaa cgaaatagaa caaaactgat tttcctttga
    17521 aacgcatacg agcaaatgca taccccgcaa gagaatcaaa gaatacagaa gtgatcactg
    17581 tccctccagc aaaaatcact gtatttttga tgtaatctaa taaagggatc gttgtccaaa
    17641 ccttaatata gttatctagg gtatagctat ctgcaaatat tctaggcgga aatgagataa
    17701 tgtctttttc gtgtttaaaa gaagaaataa tcagccacaa aaaaggaaac gcaataacca
    17761 acaatataac tagcatacta atcgttcgaa tgattttttt accactaact gcttttcgac
    17821 tattcacagt aactgtaatc gagctttctg gtgccataca ggtttccctc ctatttcatt
    17881 tgtttttctt tccgtgacat gtacacatta gtggagatag ctatagttgc aacaatgata
    17941 aacaaaaccg aagacagtgc tgatgcatag cccaattcat aaggtgctgt aaatcctcga
    18001 ctgtaaatat attgaacagc tgtttctgtc ttaaattgtg ggccaccagc ggttgcaaca
    18061 tatacttggt caaacacttg catcgaacca atcagattcg ttaaaataca aaaacctaag
    18121 gagggaacga tctgaggtag cgtaatgtta aaaaactgct gtcgatttgt tgcaccatcc
    18181 atttgagcag attcatacaa agagggagag attccctgaa tagcagtcaa gagaatcgtc
    18241 atagtaacac caaaattctt ccaaacagtc atgatggcta ccgtcggcat agcaagggtt
    18301 ggatcgcgaa aaaattgagg gatcggcaag ccgacttgtg aaaacaagta aggaatcgcc
    18361 cctacattcg gatcaagcaa catagaaaaa ataatcgcaa ttgacgtcaa tgaacaaata
    18421 actggaatgt aaaatgtcga acgacaaaaa cgattgaatc tggtattttt agataatgct
    18481 actgctgcta ataaaccaac gataatttgt gttggcgttt cgagtacggt gaaatataaa
    18541 gaatgaatca acgaattaac tgctcgttca tcactaaata tacgtgtgaa attttcaaaa
    18601 cttacaaaat ttcttgatgt aaagaaaata ttcaaatctg taaaactaat agcaaatgtt
    18661 cctaaaagcg gtatgacagt aaataataat aaaactaaaa aagatggagt taaaaacaaa
    18721 tacgcagcac gatcaggacg attccaatat ttggtgaatc cgttcatata gtccacctca
    18781 tctttcattt tgataaaata tacaatcttg gtttaaacga atctgaataa gatatccgaa
    18841 caatcgctgt gatttttgca ataacaccta tctgcaaata ctaggtcgat cattcagata
    18901 tctgtatctt gccaacagtc tatcctgtat aaagttctgt actatttttg cagtaattga
    18961 ttgatggcat catctgcatc gttcatgagt gtctcaggat cttctcctgc taataatttt
    19021 tcaatcagtg gattgatgat atcgttattg atcgcagaga tttgggtaaa acctggtaaa
    19081 tatggctctg catagtccat ttgtttcgat aattcagata caataggatc attttttact
    19141 tcttcatctt ttgctactga atccaagtag gcaggaaaac cattttcgat actccatttt
    19201 ttaccaattt cagttgtatt ccaatatttc aaaaactcat agacagcttc ttttttatta
    19261 ttatctgtac ttgccggaat ggcatatcct acgccatcta aaatagctgt ttttttgtct
    19321 gaatcactcc cttgtggtaa ggtagtcaca ttataatcaa tctcgtttgc tttcaatccg
    19381 ttatttaacc atggcccatt gatttcaatg gctagttgac cagcattcat taaattgtcc
    19441 atttcggcgc cagaaatcga ttctggacca gtcttgtcta catggatcat tttttggatc
    19501 gcttttaacg ttttaaggtt ctcaggagac gcaaattcag acttagtata atcatcgttc
    19561 actaatttac cgccatttgc taacatccag ttatataacg gtgcagttcc atccttattg
    19621 aagacaaaac ctgatacatt tttactacta tcagttaatt ttggtgccat ttctgtcaat
    19681 tcttcccaag tagtaggtgg ttgttcagta tctaatccag cttgtttaaa caaatcatta
    19741 ttccaataca tatacatgcc ttgtacctgc atggggacaa aatactgttt accttctact
    19801 tcacctaatt caatagccgt ttctttaaaa tcagatttat ctactccatc atattcccag
    19861 aaatcgtcta atggttgtac cgctccgttt tctacatact gtgcaaaatc tggataattt
    19921 aatgcaataa agtcaggtgc ttttttagaa gaaatcgctg taggaagttt ttcattcaaa
    19981 ttggcccaag tcatgatatc catttcaatt ttgatgtttt tatcatttgt tttattaaat
    20041 tcattaacga tttcttgcaa tatttcacca tcagatgcag taaacccatt ccaaaaagtc
    20101 aaagtcactt ctttatcaga atcagattct gctgaacttc cgccaccgca agctcctaaa
    20161 atacctgttg cagctaccaa caaagagcca attaaccatt ttttaccttt cattttttgt
    20221 tcctccttca tctgataaac gacatctgac cacattccaa gatagcggtt tcaataaaca
    20281 attgattttc ccatcttcag cttttacatt tgttgaatga cttggtttta cttgttcact
    20341 tttaggaaga ttagttgctt ttgtatcaaa tccactcatt tcagaaaaat caatcactcc
    20401 ctctaatgat aattcagaca agtgaacagt tagattcatt tcttcttctc cacggttaac
    20461 tgcaaatatg acaagttctt tgtcatttat tacggctata ctttctaaat acggcacttc
    20521 ttcaaaatct tccgtcgcat agtttgctga acgaatttgt gggcttaaca cctctccttg
    20581 tccatacaca gcagcttgca taaatggata atagatcgtc tgtctccaag cttctccgtt
    20641 tgtttctgtc ctgattggtg caataacatt taccaattga gcaagacaag caatctttac
    20701 tctatctgca tgtttcaata aagtgattaa tagacagcct aatagtaatg catcttcaaa
    20761 attatagtta tcttctagta aaggaggagc aatctgccaa ggcgctgctt gctcatcttg
    20821 ttccgctgaa tgataccaaa tattccattc atcaaaggac aggttgatct gcttcttact
    20881 atgtttcttc gccttgactg tatcgcacat cgctgtcact tcagaaatga accgatccat
    20941 gtctaaggaa cgtgcaagat aatgagaaag atcatttttt tggttgccat aatattgatg
    21001 cagagacaaa taatctattt gatcgtacgc ttgatcaaga actgttaact cccaatcacc
    21061 aaatgtaggc atttgccgat tcgaacttcc gcataaaacc acctcaattg aatcatctac
    21121 gagtttcatc gccttagccg tttcctctgc taaccgtcca tattcttccg ctgttttatg
    21181 tccaatctgc caaggaccgt ccatttcatt ccctaaacac catgtcttaa ttccaaaagg
    21241 gctctcttga ccattcgaac gccttaaatc actccaataa gttcctttag gaaaattaca
    21301 atattcgact aaattacgag ctgcatctac tccacgagta cccagattga cagccatatt
    21361 gatttcggcc cccacatgct gacaccagtt ataaaattca tgaatcccaa cttcattcgt
    21421 ttcaattgtc cgccaagcta aatccaacct tctaggtcta tttgctatgg gcccaatact
    21481 gtcttcccaa cgaaatcctg acacaaaatt tccaccagga tatctaatca tgggaatgtt
    21541 caattctttt accaagtgaa tgacgtcttg gcgaaatcca cttttattag ctgatggatg
    21601 atctggctga taaatccctt catatactgc tcgtcccata tgttcaataa atgaactgta
    21661 caaccgcgga tcaactttgc ctacacgctt gacactacta atcaacattg tggcttccat
    21721 aactgacctc ctttaattta tacatgaata atacacctcg ttgaaagcgt tgtcaataat
    21781 ataaatgtta aacttactta ttttttatcg attttaatta accaaaatat taataatgat
    21841 taaaacaaat aatttaatat aacttttatt aacaaatatg ttaatatata agaaaaacct
    21901 tttaagaggt gatatatatg caactacaaa taaacgaaga atccttaccg gtatatgaag
    21961 cattagctag taaaacaagg atcaagatca tccagctact ttccaaaaaa aagatgaatg
    22021 tcaaagattt ggcaaaagaa cttggagtga gcagtgctat cacgacgatg cacgtgaaaa
    22081 agctagagga agcaaacatc atcaaaacag aaaaagtagg acaacaaaaa atttcgagcc
    22141 ttcgtgtcga taaaatcgat atcagttttc cagaaaaaat attcaatgct tttgatacga
    22201 aagaaacatc gatacctatt ggtcactata caaattatgc aattgaacca acatgcggtt
    22261 tagctacgat ccatgatttt atcgggaaag tggatgaacc acgctatttt atggatcctc
    22321 gaaggatgga tgcccgcatt ctctggttta ccagcggatt tgtcgagtat caggcaccta
    22381 attttttaaa tacagaggat acattggaga tgttggaagt ttctgtggaa atttcttctg
    22441 aatttccttt ttcaaatgac aattggcctt cagatatcac tttcagccta aatggtgtgg
    22501 agctaggaac atggacgagc cctggagatt ttgctgatat ccgcggtaaa tatacgcctg
    22561 attggtatcc agataatctt aatcaatacg gcttgctgaa gacgatccga attaccaaac
    22621 acttgacgaa tatgaatgga gaacccttgt caaacatcac gatcaacgac atccctaaag
    22681 agcaagacac ttggcatttg cgaatcgaag tgaaagacga tgccaaacat gtggggggct
    22741 gtactttatt tggtaaagga ttcggtaatt acgatcagga tatcaaatta aaagtttact
    22801 acagctgata tttttctttt ctattttata aaacaaaaac agccaatccc tatgaaggga
    22861 cggctgtttt tgctttattg ttatttccag aaatcatcca aaacggaaat tggcaaatgt
    22921 cgtttatgtt gtgtcttgtt ccaccatgct tcgatttttt cttgtgcttg agcgggaact
    22981 tcttttcctt ctagataatc atcaatttca ttatatgtga cacctagtgc gacttcatct
    23041 gcaatcattg gtttcccatc ttcaagatct gcagtcggaa tttttgtgta taatttttcc
    23101 ggtgcattca atgcttgaag caattgtttc ccttgtcgtt tgtccaaacg gaataatggc
    23161 aaaatgtcgg ctccaccatc accaaatttc gtgaagaatc cagtaatgtt ttctgcagca
    23221 tgatctgtcc ctaatacggc tcctgctctt tctccagcta cggcatattg cgtaatcata
    23281 cgttgacgtg ctttgatatt tcctttgtta aaatcgctga ttcccacacc tgcttcgctt
    23341 aatacttgta cttgtgcatc aacagctggt ttgatattta ctcgcagact aacatcgggc
    23401 tggataaagt caagagcagc ttttgcatct tcttcatctg cttgttcacc ataaggaaga
    23461 cgaacagcga taaattgata gttttcatct tttgtttctt cacgcatttc ttccatcgtt
    23521 agttgcgcca aacggcctgc taaagtagaa tcttggccgc cgctgatacc tagtacaaaa
    23581 gttttaagaa acggatattt tttcaaatat gctttcataa agtcaatact tttacgtatt
    23641 tcttcttttg gatcaattgt tggtttgaca cctaattcat tgataatttc tgtctgcagg
    23701 ctcatttatt ctcctcctat gcttctgtat gaagttctgc tacgcttttt ttcattcgtt
    23761 ccatgatagc cattttatgg ttccagcatt cagtagacag atcgactggg tatttttgcg
    23821 gattcaagtc tcgtttatac tcttcccata gtgaatctag attttctttt gtatattctt
    23881 tgatttcgtt tagtgcaggt aattcgtaaa ctctttttcc ttcgataaag atatcttgta
    23941 aaactgggcg agctgtaaag tctcgaacag ttttattgat aaacgtatgc actggatgga
    24001 acatgaagat agcttcttct tctcgagggt cttcatccca taatgtcacg tagtctcctt
    24061 cagatttccc atcaaaattt cgagtgatgc gccacacttg ttttttccct ggagtagtta
    24121 ctttttctgc attgcttgat aacttgatcg tatccatcat ttttccttca tcatcttcga
    24181 tcgatactag cttgaatact gcaccaaggg ctggctgatc ataggctgtg atcaattttg
    24241 tcccaacacc ccatacatca attttagctt tttgcatctt taaattcaaa atcgttgctt
    24301 catccaagtc attcgaagca tagatttttg cttccgtaaa tccggctgca tctaattgtt
    24361 cccgaacacg cttagatata taggccatat ctccactgtc gatgcgtacg ccttggaagt
    24421 tgattttgtc acctaattct ttcgccacgc gaatcgcact tggcacacct gatttcaacg
    24481 tatcataagt gtcgactaag aacacgcagt ctttatgggt tttagcgtag gccataaatg
    24541 cttcataatc atttccataa gattggacta acgaatgagc atgggtcccg ctagctggta
    24601 tcccaaatat cttcccagca cgtacattgc ttgttgcatc tgctccgccg atatatgccg
    24661 ctctagttcc ccagactgct gcatccagtt cttgtgcccg tctagttcca aattccaata
    24721 gtggatcttc tccgatgaca gatttgatac gagctgcttt tgtcgcgatc aacgtttgga
    24781 aattgaccat attcaacagt gctgtttcta ctaattggca gtgcgctaaa ggcccttcca
    24841 cttggattaa tggttcattt gcaaaaacta gttctccttc acgagcagaa cgaactgttg
    24901 ctttaaattc aaaattttgt aaatactcca aaaaaccttc aggatagact tctaatccac
    24961 gcaagtaagc aatatcggaa tcattgaacg tcagattctc taaataattg actaaccgtt
    25021 ccaatcctgc aaaaaccgcg tatccgtggt taaaaggcat ttctcgaaag taacattcaa
    25081 aaacagcatg taaatcagct cgacctaatt cccagtatgt ctgcatcata ttgatttgat
    25141 atagatccgt atgcagcgtc aaactgtcgt ctgggtaagt attattcatc ttgtattact
    25201 cctcttggca agttttcatt tcctacccat tataacaaaa aaaattaata ggaaaagaaa
    25261 tcttccattt tttctgaaaa agcacatgtt ttacggatat ttcttgctaa aaatgaactc
    25321 catctgctaa aactatcctt tcttattggt atttaaccta aacgagaaag aatacttatt
    25381 ttattcaatg tatccgagac acgaagaaat tagcgttttc tatttttctt acttgataaa
    25441 ataagtagat tttcctacta caatcgtagc cgttaagata ccgatgattg aacaaatata
    25501 accaaatgtc tctacaacat aatcaatggc aaaagttttt gaagcaataa tacgtctttc
    25561 cttttttgct tcttatcccc aaagaaacgg atataactta ttatgtattg aatttttttg
    25621 gaaaggcgaa ttatgacaag atataccttg cgttgaaaaa actcgcggaa aaaatatcac
    25681 acgtttttaa aaagatacaa agtaagaaag cagctgtcaa tagaatggaa acaaaaatgg
    25741 cgagacatca atcagtctcg ccttgttttt tattgtatac attagatatg gagcaggtta
    25801 ccataaagat gattattagt cgttattaaa tagaatcctt cgtatttttt tgttctatca
    25861 ctacaatatc tttatttgga cgtttgaatt gtattccggc acgttctagc tcctgtttca
    25921 ctcctacatg gactttgaca tcttctcgtg tcatttttaa aacgtgatga atcgcatagt
    25981 cgatttcttg ccttgtgatt tttttcttcg tattgatcat cacgacttgc tttttatcat
    26041 atgtgtctac caatagcagt tgtgttcgat ctaacgaaaa taatttgtaa taattaatag
    26101 ccgtattccc aaaaaagaat tccactactt taggataaaa tgtcttaata tttaaattac
    26161 tattcacagg tgtttcgatt agcttcatct cattcctctt ttctatatag tcgatttaga
    26221 tccaatctta agttatgtta ccatctttcc tagcattaag gaatcggtta caattgaacc
    26281 tttaaagaca aaaggattta ccttttatca cttaatagta taacataatt tcgtgagaaa
    26341 aaaatacaat tttttcttga ccatctcgtt acgtaaagga ttatgcttac atttgtcagg
    26401 aggaaaaata atggagtaca caataaagaa aatgtcagag atatcaggag tcagtccgag
    26461 aaccttgcgc ttttatgatg agattggact gctaaaacca gctaggatca attcttcagg
    26521 ttatcggatt tatggcaaaa aagaagtcga tcgtctgcaa cacatcttat tttatcgaac
    26581 aatggcattc aagttggatg atatccaaga ggtcttagac aatccatctt ttgaccacca
    26641 gaaagcttta atcaagcatc agcaaatgtt gctggaaaaa cgggcacaaa tcgataccct
    26701 cttgacaact gtccaacaga cacttgatat gtacgaagga ggaagaaaaa tgagtgacaa
    26761 agaaaaattt gaagggttta aacaacaaaa gttgaaagaa aatgaagaat cctatggaca
    26821 agagattcga caaaaatacg gaaaaaaaaa ggttacagca tccaatgaaa aatggatgaa
    26881 tatggataaa gaaacgtatg aagaaatgca aactgttgaa aagcagatgt tatttgatct
    26941 cactctttac ttaaaagaac cagaagatga aagcttagca gaccgtatct tccaagctca
    27001 taagaagtgg ttctcttaca gctggcctga atatcagcca aaagcgcata aaggactagg
    27061 aatgatgtac gaagccgatg aacggttcac tgcatattat gacgagcgaa gtggtcatgg
    27121 tgcagcaaaa gctttaaatg aaattatcca aaaaaaggcg taatctttta catgatccaa
    27181 caaaaaagta cacgtaaaat gacctaataa caaaacagca gacacccaag ttagattttt
    27241 atgctaactt ttggtgtctg ctccattctt tcacaagttt tttcatccta ctttaaatat
    27301 attcatcgtt gaataaacgt ataaggtatt ttcactttat cctttttttc tgtaagaaac
    27361 aattgaaatg ccatttctcc gcaagcttcc agatggtggt cgatggttgg gaaaccaaga
    27421 agctcgctga tcagcaaatt atcgcgtcca atcaccactg gtgtcttatc ttgcagatag
    27481 ctttgcaaga tgcctgctgc aaccatatcc gaatttgtaa agacaccatc aactccaagc
    27541 tctttaaaaa agaagcctgc ttgttttcct ttctctgcat ctatacaatc ccaaaaaaca
    27601 agtgattcat caaaagatgg aaaaagttga tgacatagct gcaaagtgat ttttgagtta
    27661 taacttagcc tgccacttcg ccccaacgtg atgcccaatt gctttatacc ttgctgcttc
    27721 aaataagtaa gaccttctgt aattgattgt tctcgatcaa taaagacaca gcttgcctgt
    27781 tttcccttga tttcttcaca aaaaactaga ggtccatatt tttgatatgc caataaatca
    27841 tctagtgtat tggcacgaga cgtaatgatc aatccatcga aagctttagc tgcaaattct
    27901 tcaagatacc ttcgctcttt ttctttatga tagcccgtcg gtaaaagtgt cactttgtaa
    27961 tcataatcaa acgctgcttc aatgatccca ctgatcaact gatcaaaata cggatgattg
    28021 gtataaggca gaatcactcc aatgcttttc gtttttccat aacttaactg tctggcattt
    28081 gcattaggga tataatcgac ttctttcatg accgcaagta tttctttcct tttttcctca
    28141 tctacatatg gatgtccatt gattacacgt gaaatagtag aaactgaata acctgtgagc
    28201 tttgcgatat ctcgaatgtt tgccactgct gcttcgccac ctatacaaaa agattttctt
    28261 gtaaatagtc taccacagct ggaccgcatt caaaaaactg atacattttt ccttcatgcg
    28321 aaaaactgat ccgctgcccg ccccaatata tatcttttgt caaattttcg atttcattca
    28381 ttggtatttc ttttactata ttcctattca acccttgaaa actgtacaac gttaatacgg
    28441 atcccttgaa ctcgacaaac gcataaaaat aattgttcgt tgtgccatct gttatctcta
    28501 cataagtatt cattgcaagc cctccttttg atactttgag tatagtgtat gaaacgcgtc
    28561 tcatagcaag gagtttgttt tgccaaagaa aagttttatt cctctttcat tattaccaaa
    28621 gtctcttatt ttaaattgaa tacattataa tgatactgta aatatatctt tattattagt
    28681 gaat
//
LOCUS       DAFEOM010000027        28599 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.028, whole genome shotgun sequence.
ACCESSION   DAFEOM010000027
VERSION     DAFEOM010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.028"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            234..1874
                     /locus_tag="LP524_001404"
     CDS             234..1874
                     /locus_tag="LP524_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HBL2234466.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2028..3350
                     /locus_tag="LP524_001405"
     CDS             2028..3350
                     /locus_tag="LP524_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HBL2234467.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3412..3843)
                     /locus_tag="LP524_001406"
     CDS             complement(3412..3843)
                     /locus_tag="LP524_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2234468.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3907..4743)
                     /locus_tag="LP524_001407"
     CDS             complement(3907..4743)
                     /locus_tag="LP524_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBL2234469.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4933..5235)
                     /locus_tag="LP524_001408"
     CDS             complement(4933..5235)
                     /locus_tag="LP524_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBL2234470.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5261..5692)
                     /gene="ruvX"
                     /locus_tag="LP524_001409"
     CDS             complement(5261..5692)
                     /gene="ruvX"
                     /locus_tag="LP524_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBL2234471.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5689..5955)
                     /locus_tag="LP524_001410"
     CDS             complement(5689..5955)
                     /locus_tag="LP524_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBL2234472.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6209..6934)
                     /locus_tag="LP524_001411"
     CDS             complement(6209..6934)
                     /locus_tag="LP524_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HBL2234473.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7300..7370)
                     /locus_tag="LP524_001412"
     tRNA            complement(7300..7370)
                     /locus_tag="LP524_001412"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7336..7338),aa:Gly,seq:ccc)
     gene            7487..8239
                     /locus_tag="LP524_001413"
     CDS             7487..8239
                     /locus_tag="LP524_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBL2234474.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8255..8734
                     /locus_tag="LP524_001414"
     CDS             8255..8734
                     /locus_tag="LP524_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBL2234475.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8985..10565)
                     /locus_tag="LP524_001415"
     CDS             complement(8985..10565)
                     /locus_tag="LP524_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HBL2234476.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10558..11301)
                     /locus_tag="LP524_001416"
     CDS             complement(10558..11301)
                     /locus_tag="LP524_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2234477.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11477..12253)
                     /locus_tag="LP524_001417"
     CDS             complement(11477..12253)
                     /locus_tag="LP524_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2234478.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12501..13337)
                     /locus_tag="LP524_001418"
     CDS             complement(12501..13337)
                     /locus_tag="LP524_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HBL2234479.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13348..14277)
                     /locus_tag="LP524_001419"
     CDS             complement(13348..14277)
                     /locus_tag="LP524_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBL2234480.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14470..14823
                     /locus_tag="LP524_001420"
     CDS             14470..14823
                     /locus_tag="LP524_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBL2234481.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14897..15553)
                     /locus_tag="LP524_001421"
     CDS             complement(14897..15553)
                     /locus_tag="LP524_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBL2234482.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15779..17713
                     /locus_tag="LP524_001422"
     CDS             15779..17713
                     /locus_tag="LP524_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL2234483.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17799..19022)
                     /locus_tag="LP524_001423"
     CDS             complement(17799..19022)
                     /locus_tag="LP524_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBL2234484.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19016..20914)
                     /locus_tag="LP524_001424"
     CDS             complement(19016..20914)
                     /locus_tag="LP524_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HBL2234485.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(20908..22026)
                     /locus_tag="LP524_001425"
     CDS             complement(20908..22026)
                     /locus_tag="LP524_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBL2234486.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22109..22942)
                     /locus_tag="LP524_001426"
     CDS             complement(22109..22942)
                     /locus_tag="LP524_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL2234487.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22935..23852)
                     /locus_tag="LP524_001427"
     CDS             complement(22935..23852)
                     /locus_tag="LP524_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL2234488.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(24079..25203)
                     /locus_tag="LP524_001428"
     CDS             complement(24079..25203)
                     /locus_tag="LP524_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBL2234489.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            25386..26294
                     /locus_tag="LP524_001429"
     CDS             25386..26294
                     /locus_tag="LP524_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2234490.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            26435..28048
                     /locus_tag="LP524_001430"
     CDS             26435..28048
                     /locus_tag="LP524_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL2234491.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         8892 a         5621 c         4868 g         9218 t
ORIGIN      
        1 tatttatcta tctaacatat cctttattct tctacttttc tttctttagc atataagaga
       61 tacgcatagt aatcaagcaa ggattgttga tataaaaaga acaaataccg ctattttgat
      121 ttcttttgac tctattactc atataaataa aatttattat acctgctctt tttgactata
      181 tcctcatctg actcctatac ttcgtttgta ttaaaactta ggaggaatca ttcatgctta
      241 cagggctaaa cttattaaat aatccttttc taaataaagg aacagctttt acaaaagaag
      301 aacgggcaaa atacggaatc actggtatgc ttccaagtac tgtccaaaca ttggaacaac
      361 aatctgtcca agcttacggt caatacttaa gcaaacagac cgatttggaa aaacgtatct
      421 tcttaatgaa tctattcaat acgaaccgga cattgtttta taaattgatg ggcgaacatt
      481 tagttgaatt catgcctgtc gtttatgatc ctgtcgttgc ggattcaatc gaacaataca
      541 acgaaatttt cttgaaacca caagatgctg tatttctttc aatcgacgaa ccagaaaata
      601 tcaaagcaag tttgaaaaac gcagcagatg gacgcgacat tcgtttgatc gttgtgacag
      661 atgcagaagg aatcttaggt atgggtgact ggggtgtgaa cggtgttgac atcgctatcg
      721 gtaaattgat ggtctacact gccgcggctg gtatcaatcc tgcacaagtc ttgcctgtat
      781 caatcgacgc tggtactaat aacgaagaat tattgaataa tccattatac ttagggaatc
      841 gtcatgctcg tgtagaaggc gaaacttact acgaattcat tgatcaattc gttcaatcag
      901 ctactgaatt attcccagaa ttgttattac attgggaaga ttttggacga ggaaatgctg
      961 caacgatctt agaaaaatac gaagataaaa tcactacttt caatgatgat atccaaggca
     1021 caggaatcgt tgtactagct ggtgtattag gtggattgaa tatatcaggt gagcaattga
     1081 aagatcaaac gatcttgaca tttggtgctg gtactgctgg tgtaggtatc gcaaacatcc
     1141 ttttagatga aatgatccgt caaggtgtac ctgaagaaga agcacgccaa catttctatc
     1201 aagtagataa acagggttta ttgtttgaag atacaaaagg attgactcct ggtcaaatcc
     1261 cattcgctcg taaacgctca gaattcacta atgctgatga attgacaaac ttagaagcag
     1321 ttgtaaaagc aatccatcca acgatcatga tcggtacttc tacccaacct ggagcattca
     1381 ctgaagaaat cgtgaaagaa atggcggctc ataccccacg tccaatcatc atgcctttgt
     1441 ctaatccaac aaaattggca gaagcaaaag caaaagattt gattgaatgg acggacggaa
     1501 aagctttagt tggaacaggt atccctgctg ctgatgtgga atataacggt gtaacttacc
     1561 aaatcggtca agcaaataat gcgttgatgt acccaggttt agggcttggt ttgattgctt
     1621 caacagctac acgagtaaat gctgaaatca tttctcaagc tagtcgagca ttaggcggaa
     1681 tcgttgatgt aacaaaacca ggagcagcta tcttgcctcc agtagcaaaa atcactgaat
     1741 tctctcaaac gattgctgaa actgtcgcaa aatcagttgt tgcacaaaaa ttgaatcgtg
     1801 aagaaatcac ggatatcaaa gaagctgttg aatctgctaa atgggttcct gaatataaat
     1861 ctttagaaga ttaagattgt tatagtaaaa gaacatacag aaactttgta tgacgtctaa
     1921 aaagaaaaaa gcgactgcgg caaaacagca gccagccaac tactgctctt ttgccgcttc
     1981 tctttggctt ttttcatacg atacagaata agaaaggatc tgtaaaaatg gcacaaaaaa
     2041 caacaaccgc aactgcagtt gaggaaaaga aaaaagaagg gttttggtca acaaaaatta
     2101 ccggcgtaag tttgcccctt tatttgatca tggtccttgt cctgatcatt gcaatggctt
     2161 taggaaaatt accgacgaat atgattggcc caatcgctgt tttggtcatt ttcggtaatt
     2221 tatttcattt tatcggaaca aaaatcccta tagtcaaaag ttatttaggt ggtggctccg
     2281 tttttgcgat ctttgcctct gccgcgattg ctacatttgg gatcttacct gagtatgtag
     2341 taaaatccac agaaaacttt gtcagcaata tgggcttttt agacttttat attgctgcct
     2401 tgattactgg tagcatcctt ggtatgaacc gtaatctatt gatgaaagct tctgttcgtt
     2461 ttattcccgt tgcacttatt tcaatggtcg tctccttttt tgccgtagga ctcgtaggta
     2521 tgcttatcgg aaatggtttt gcaaattccg ttttatatat ctcattgccc gcaatggctg
     2581 gtggtgttgg agccggagct gtacctttat caacgattta tgcaaatgtt ttaggtacgg
     2641 acgcttcttc tatcatttcc cgcctgattc ctgcgacagc aatgacaaat gtcctagcta
     2701 tcattggtgc agcgttagtt gctcgtgcgg gacaatcaat gccaaaattc aacggtaatg
     2761 gtaaattaat ggaaaaaggc gaccttggcg atggaaaacc tagaaatgta aaaccttcca
     2821 ttccacaatt aggtgtcggt ttgatggtat cattgacttt cttcatctta ggacaaatct
     2881 gtaactattt tgttcctaaa gtccatgctt acgcattcat gatcattatc gttgttatct
     2941 gtaaaatcgc aaacctatta ccagaatatt acgaagatgc agctatcatg ttcaacaact
     3001 tgattgtcaa aaacttgaca gcagctgttc ttgccggtat cggtattgcg ttattgaatc
     3061 taaatgtatt ggcttctgct ttgacatggc aatttgttgt cttatgtttg acaagtgtta
     3121 tcgcgatttc agtagtttca ggatttgtcg gtcgtttgtt tggtttatat ccaatcgaat
     3181 caactattac tgctggctta tgcaacaact cgatgggtgg tacaggaaat gtagctgtct
     3241 tatctgcagc taatcggatg gaacttattg cttttgctca aatgggcaat cggcttggtg
     3301 gtgccatcgt tttgatcatc tcaggtttct tgatgcaatt actttcttag tcaaaaaaaa
     3361 tacctgatca tcaagtatcg gcttgatgac caggtatttt ttgataaaaa tttacaggat
     3421 tttcaaaaat cttttttgca ttttatcgga atgataaccg atgcgttcat aaaatcggtg
     3481 tgcttctact ctgtattctg cagaattaag tcttattgct gatattcctc gtcttttcgc
     3541 ttcttctacg ccagtcatca aggcctttcc gattcctctt ttttcagcat gtttagacac
     3601 agcaagtgcc ataatattga acatcggatc gctgaaaatc gattcatagg actccgcttg
     3661 tacatagcca gctgcttttt tagaagtagg atcttcatag accaagaaaa aatttttttc
     3721 tggctccttc aacagtttct tcatttgtct caccgtaacg gttaatggta catcatatcc
     3781 taattgttcc gcattgattt ctcgtagtgc ttctgcatct cctaaacgca tttcccgcaa
     3841 catccaaatc accagccaat ttttattttt ttaaatgaat ttttttatca cagtatactc
     3901 cttttttcac ttaaaaaaag acttttcagt gattttgact tgcttgaatc cagatccttc
     3961 taatgtttgc aagatgacga aagcattgta aagattgatc aaaagaacca cgatgacaat
     4021 cgcttgaaaa ataaaggtca ctttttttgg agacaaaacc ctgctaaatc ttgctcccca
     4081 caaacctcca ataacagcag cggggataac ataaaacaac atagataaat caaagcgcat
     4141 aaagctactt gttaaagcaa tcgttaccaa ttttgccagt tgagaaaaga aaatcgtaga
     4201 tagagaatat agtgttgctt cttttattgg caatgcaaac atcagcatca acaaagaaac
     4261 attgactggt ccaccaccaa tacctaaaaa actagctaaa aagcccagaa cccccccaca
     4321 tataaggtac caagtccatg acgtcaaatg aaattttggc tgatgatgct tggtgtagaa
     4381 gaacgcaaaa accaaggtca ataccgtcaa aaaaatttgg atcagctgca catgcttctc
     4441 gttttcaaat aactgtaaaa aaacttcgaa tacaatgttg ccgactactc ctccaacaac
     4501 agctccacta gatacccaac cgacaatctg ccaattcagt gatttcccgc ttgatacttg
     4561 tcgcacagtc gaaacaatgg acatcgtgaa taccgcaact gttgagtaaa aagaaatccc
     4621 tgcaacagaa tgggcgccga taagatcaag aatcggtttg atcaacacgc ctccacccat
     4681 cccggaaata gcgccaactg tgttcgccag aacgatcacc acaaaataaa tcagaccaat
     4741 catcttgtat ttcctttctc ttgtttatat cttttagcga ttgttactct tttcttttat
     4801 tatacagtaa aagaaacaaa cgaggcttcg aaagcttttc tactttttaa ggaaatttag
     4861 tgctaaacga aaaaaccggg ctctactttt cagagagtcc agcttttctt acaccttatc
     4921 gattcttttg ttctattctt cttccgccat gaatgtattg aatacttctt cgatcatatc
     4981 ccattctgcg tctgtttcga tttgttctaa ctcgccttct gttccatctt cgttttcgat
     5041 gtatgcataa gcttgtaatt ctacatcttc atcttctgaa acaccagctg gatataaaag
     5101 aacatagtta cgtccaaatt cttcttgtcc gtcaactgtt agcaagattt cgtataatgt
     5161 ttcatttcct tggtcatcca ccaatgtgat gtgttcgtgt ccttcgtgtt cgtgggaatg
     5221 gttatgttct gtcataagat tacctctttt ctttttatta ttaaaataat tgcttacctg
     5281 agccgtctaa ataattttgt aaaatcatca cagctgctaa tttatcgatg actttttttc
     5341 gtttcgctct tgaagtattt gcctgctcta ctagcatacg ttctgcctga acagtggtta
     5401 gtcgttcatc ttgataatca accggcaagg agaatagttc tttgatcttc tctccatatg
     5461 acatggaagc ttctgccctt ggtccgatgg aattgttcat attcttaggt aatccaacta
     5521 caaaacggtc tacttcgtac tgcgccacta attccttcaa ccgatcaaaa ccaaactctt
     5581 cttctgcctc attgattcga atgatttcta ctccttgagc tgtccagcca aagggatcgc
     5641 tcacagcaac gcctactgtc ttagaaccga catccaagcc cattactctc ataaattcac
     5701 tccatgattt gagaggtagt ctttgacaat tacttccatg atttcatcgc gttcgtgacg
     5761 gcgaatcaaa tttcgtgcat cacgataacg aggaatgtag gccgggtctc cagaaagcaa
     5821 gtagccgaca atctgattga ttggattgta gcctttttct tccaaagccc gataaacaat
     5881 cgctaatgtt tcgctcactt cttttttgcg actatcgtca aaaccaaaac gtacggtttc
     5941 atctgtaaaa cccatgattc tacacctctt tcccgcaagg taattccagc ttaccttcta
     6001 cacccttatt ttactcgaag aaagggaaaa ctacaaacaa atattccaaa cttctgaata
     6061 aattaagaaa cacgatttgt ctgatttttc cttttttctg tggctttttc aaaagccctc
     6121 tttccataaa gtatacgcta tatttctttg aaagtatgtt tttacacaat tttttcataa
     6181 agttcataaa aaattcgaat tgctgctact aatttttcgt tttcgttgct tgccctgtct
     6241 cataattgat ggtgtagcca tcttgaacat tgtagataaa gacattgaat tcaatttttt
     6301 tgtcttcaat gctttctgct tccaactgca cacctcttgc taccagttca tttccctcga
     6361 aatgaggagt gacacgatag cgtacatgat gatttgtttc cttgatatat gccgcaatat
     6421 cattttcgtg ggcaagcatg tacggagcat tcaacgaacg tgtccctgtc attaagtttt
     6481 taatgttatt attttgacca gtcaactgga aaccaatcaa atggctgcgg ttgtataacc
     6541 attgtccgtc acttaatttc ttttgtttcc aacctgtcgg atcaatatac aaaggttctc
     6601 tcttttcagt aggaaacaag tcttttccca acatcgcatt ggcttgtcct actctgttca
     6661 atttgtctat atttgaatag ctttcccatg tccctttgga aagactaaga tcctcttttg
     6721 taaactccgg ctgattccca ttgatttcta tttcttgatg ttttccatca tattctggca
     6781 catccgctac ttgagaagct tgcggtgtgg aatcaaaaat ggttgtaatt tgttggaaga
     6841 tatctgaatc tttttgtgtg gtggaaatac cgagtgcccc tgcaatcaca gcagcgacga
     6901 tccccacgat cgccttggtt gtttttttgc tcatttcttt actcctttac taacagttcc
     6961 ctaaatctta acatatgttc ccctataaat aaatactgga aagaaataga aacagtaaaa
     7021 aaataaaacc agtttttaga cggcttaaga atacaaaaaa agagacggat tcatcgtctc
     7081 tactcaatca caaaacacta tttacaaggt atttattatc attcaaaaaa tttttcttaa
     7141 tactcaaaac taaacggctt ctgtacgacc tcttggtaaa cagtgttcaa aagcttgcat
     7201 ctgcggtaat aagtccaacc aagtcaaaaa cataagaagc ttttttgctt gactattctt
     7261 attacttgat gcagggcagc tttttcacag cctcttttaa gcgggtgacg ggaatcgaac
     7321 ccgcgacatc agcttgggaa gctgggattt taccactaaa ctacacccgc gatacaaaaa
     7381 cagtatacgc ctctttcact ccttcctcaa gtttttttat tctttacgct tccgatatgt
     7441 tttgtagtaa aataaaacta atcaatcaat tattggagaa gatgctatga cgggaaaaga
     7501 attagatctg ttgctggaca cttgtttact cgctgggaaa atcatgatgg aaagcaatgc
     7561 ggagatgtat cgggtagaag atacgatgag tcgaattgct ttagcttctg gaaatttccg
     7621 tttggtcagc tatgtcactc aaaccggtct ctttattggt cttgatcgta cctcgaccat
     7681 ccgaatggaa caaattacca accgttcgat taatttagaa aaagtagtaa aagtaaatga
     7741 tctttcacgt aaatatgttg cttcagagct aacattagaa gaactttact gcgaactgca
     7801 agcaatcgac cgagaccgtt ctttctttcc tgtctggttg caaatcgtta gcgcggcagt
     7861 gatcagtgga acaattatgg tgttatttgg cggaaagatg accgatttac ctgtgacttt
     7921 agtcattgga ggattgggat acgcagctta tctatatagt ttgaaatttt ttagaatcaa
     7981 atttttatcg gaatttttat cttctttatt gattggcata gccgctatct acagcgtacg
     8041 ttttggctta ggaagcaatc aggatctaat cattatcgga tgtgtgatgc ctttagttcc
     8101 tggagttcaa attacgaacg ctatccgtga tttgctagct ggacattatg tctcaggagt
     8161 ttctcgcgga acagaagcga tgatgactgc tacgatgatc ggttttgcga ttgcttttat
     8221 tttccagctt ttttattagg aaaggaacac aagtatgggg acgatattta ttcagttttc
     8281 ttttagtttt ttagcgactg cggcatttgc cgttattaca aatgtaccta ggcgttcatt
     8341 gatttgttgt ggtctttctg gtgcttttgg ctggatgatc tactggatct gtgttcaaat
     8401 aggcggatct gctgcttttg gttcattgct tggttcttta ggagtagcat tcatcagcga
     8461 tcttttctct aaacgtttga aaatgcccgt aacgattttc aatattcccg ggatggttcc
     8521 tctagtccct ggtggtttag cttatcaagc ggttcgaaat ctcgttactg aatcctatcg
     8581 agaagcggct cattatgcgg tccaagcaat tatgattgct ggagcaatcg ctttaggtct
     8641 tgtattatca gaagtcttta accataacat ccgtaatttc aaagtaaagc gtgacgcaat
     8701 catgatgaaa cgaaaaaaac ggctttttaa ataaacgaag tgtaacaaag aaaaggttgt
     8761 gaaagagacg ttcagccttg agcgatgaca aaatatctca caaacattgc gacaagtaac
     8821 cgcaggagca ataagaagta attatgagta ttaaaaaaag ttttgtaaaa atagcttctc
     8881 acatttttac ataaaattga cttaatactg agaaactgac ttttgaacac cctctatctg
     8941 taaaaaaggg gctgcgacat gatttttgtc acggcccctc ttctttattc aaattttttc
     9001 caaaacgttc gttgataata aacgatccat agagcggaaa gtattagaac taaaccgatc
     9061 acaggaatat tcactaacca aaatggataa aacaaagcca acattccgta taagacgata
     9121 agaataatac tgatgatcaa tgaacccatc atagttgcga ctaaacccaa acttcctgaa
     9181 cctctgttaa ataattggtt aatattggtc cagttcagca gcagcaaccg ataatctcta
     9241 gcgaaataac gtagtgacaa taagtagcag ccgaataaag aaccaaatag caagctaggg
     9301 ataaaaaaca aaggcagctg aaaagacagt ccgcttaata atgcgatccc accagttaaa
     9361 aaactctgca atatccatcc gaatctgaac ttttctttta aatattggtt catagagatt
     9421 ggcaaagaac ggataaacag aaaattttct tgatccaaag aaataagatt gctgataaaa
     9481 gaagtctgat taactgttaa gaaagccaat gcgatcccta ccaggaaaac aacaccgatt
     9541 aacttattat ctaaatcagt taattttatt tcgcctccta gggcaaaagc aagaataaaa
     9601 atgattggag ttaacaagga agaagaaaga acttgcataa tcaaattagg ttcttttact
     9661 aattgagcat tgtaactaaa taaaagctga tgcacatttt ggttcttttt atgggttctt
     9721 tttattttga actcactgtt tgaatcagtc agttgctctg taagctttgg tactatcagc
     9781 tgttttacag caaggaaaaa tcccagagcc gtgatagcta gtattcccca acccaataaa
     9841 ccttgttgag aaagtggctg actggcagca tcatagaatg gcaataatat cgaaatcgga
     9901 tgccgatctg ttaactcatt ttcaatagtc cccgtttgat gattcatcca catgattcca
     9961 gcaaatgcga tcagcatcga gctgccaagc aagatactag aaactgtttt tctatgtttt
    10021 ttaaacactg ctgttcgagc caaaccaaaa acaatcaaac tagccagaca aaaaagaata
    10081 atcaaaaata gagaaaacaa aaggattgag ataatgatag taagcaaaat ccctagatca
    10141 gctcgccatc cggttaaaat aaacatgact agaagcggaa aaacaaaagg aatgatggtc
    10201 aaagcaacta ccaaaatctt tgctgaaaaa acctgttctt gtccaaaagg cagaggcaaa
    10261 aatacttgca ggtcatttcc ttcaaaaaag atattataaa tgactgatat actttgagtt
    10321 aaacctaaca taccaaaaag tgccacgtaa taagtaaaga aacctggcaa tttaacaaag
    10381 ttcatagcaa acatagcaaa tccgtaaatc agtaaaaaaa tcagccctga aaaaagattt
    10441 tgagacaaaa gatagatagt taatcgcttt cccttttttc ctttctctct tgctttattc
    10501 gtgagctgtg gattaacata taaaagatta actcgaatta attctaataa ttgtcgctta
    10561 tacatacggt ccatctcctg tcgcttgcct tccagccatt ttcaaataaa tcttttctag
    10621 cgtttcatca ggtgagccat tcaataattc actaactgcc ccctgataaa tcaattcacc
    10681 tttcttcaaa atagccagtt tatcgcataa ctgttgtgcg gtatccaata catgtgtaga
    10741 aaaaatgact gtttttcctt ttgcggcatg agccttcatc atttctttca aatcaaatgc
    10801 tgcttgagga tcaagtcctt gcaaaggttc atccaatacc caaatatctg gatctggcag
    10861 caacgtgccg atcaaaatcg ttttttgacg cattccatgt gaaaaacttg cgattgtttc
    10921 attttgatga ctatacatat caaaaagcgc tgtcaattct gctaaacgct tttctttttt
    10981 ttgtgtgtct aattcatagg cagctgaaat caagtcccaa tattctccag ccgtcaattg
    11041 taaaaaaata tctggggaat caggcacata accgattttt tgtttgatac tcaatcgatt
    11101 ttccgtcaat ttcattccat ctactaaaat cgttcccgat gatggttcaa taatactaac
    11161 taaactttta atcgtagttg attttccggc accattatgt cctaaaaatc caaaaatttc
    11221 cccttgtctt atcgagagag acaagtcttt taatgcttgt tttttcccgt aacttttact
    11281 tacattgatg aattcaatca tttctgtact ccttttgttt gttattactt tttattgtac
    11341 ccttcgttat tggtggagta aatgtcaaaa agagtgcata acaaaaaagg atagaaacga
    11401 ctaggctgct acttgggttg ttctcttcta caggaaaaca ctcttctaac aggtaagata
    11461 tgagtctttg ttttctttaa aatgattcat tttctatctt ttcttgcata aaattcttta
    11521 gtttatcaat acttctcttt cccatctcta gttgttctcc atttgcaaat gttactattt
    11581 cgtttactct gctaatagat gtaatgtttt cagtattaat gatataagct tttaaatctt
    11641 tacataaata ctcatattcg agtgattttt tatatttttt aattgtgccg tttacgagaa
    11701 tctcttcatc tttcaatact aggcctattt tatttttata acctggcatg ctcattagat
    11761 aaaggatttc atcgtaatgg atatagtgat cgctattacc ataacggaca gttgtccacc
    11821 tttttttcgt agcatgttga acaggatagt tttctttttc tttcaataag taactttgga
    11881 tgcttttttt atttttttca taagtgaatt cttttatcaa ataaccacta ggcgtaatct
    11941 ttcgattaat aacagttatt gctttattcg gatgagacgt tagataaaca attttacact
    12001 tttcattctt ttctctaagc atttcgccta gttcgatacc gctataatag gcgttcaaat
    12061 cgatgtctag gaaaaaaata tctgaggaat agatttcatt atttttaatg aattcaacaa
    12121 attgtataac gttcaatccc tgaacgatct caaatttcca aggacttaat aaaattctgg
    12181 attcttccaa aatttctact agcatttttt caaaaaccaa actatcatcc aaaataaata
    12241 tacgcgttac cataaaaagt acctccactc gatattttac tatcaaaatc atttcataca
    12301 acttttcaac atacatgtta ggaataaatt gtaattctct tcacaatctt ttccaacacc
    12361 tttgaaaccg atatcaaata ctttcaggat gctgcttcta aaataaatat caaacaaatg
    12421 aatacatcat gcaaaaaaag ccagacaacc gacgtccttg cgtgtcagct gtctgagcta
    12481 tgtttattta ttttgttctt ttaaaaagaa cgatacacat actgattttt cggagcagag
    12541 actttctcag cgtttgtaac aactacagat gggatttcac gcttgaatgg ttgataatag
    12601 tctgattgga tggttccttc tattttatac cattcatcat ttttaaccgt tactccttcc
    12661 ggcatatgcg tcaacaaacc aaagactcct gaatctgcca cacaatggat aatgccgaat
    12721 cggaacaaga agatatctgt attgctatcg ttcggtgttt ggtaaacaaa tccttcatag
    12781 ctgatttttt tgccaatgaa ttcgctaggg taattataaa ttagctccat aacttctaaa
    12841 tagttttcat ctgtgatctt gatagtttgc ttatccccat acttttcttt caactcattc
    12901 atttggtcta agtagtctga tttgttgaaa taaatactgg tatccggctt taagtattgt
    12961 gtctgcatct ccggatcccc tactgattct ttacttactg ggaaattaaa tcctttggct
    13021 tctacgatcg tagtgtcgag actaactgtt ggaaacaacg tccccacgat tactggcaaa
    13081 gctagcagta cataagcgat tccacgttga taaggttttg acaacccatg gtcatgatcg
    13141 tgctcgtgtg ccttttcttc tgcttttccg tttttgaccc acaaatacag ctgaactaaa
    13201 gccaaaagag cagataaaat cattgaaaga atagctaagt agcgataatg gacattgata
    13261 tattgatcga gtttgccaga aacttgcaag tacatcatca attcgaaata cccagataaa
    13321 atcaaaaacc gcagcatatt ttccctccta aataaataat gtgaatccaa taacgctaag
    13381 ggcaatcagt ccgatcagcg ataaaataaa gcgtcctgta aaatatcgtt tcatcatcag
    13441 tagatttttg atatcgacca ttgggccaaa gaccaaaaag gcagctacag gtgctgtgcc
    13501 gaacagactt aacaaagaag atcctataaa cgcatctgct tctgagcaaa gagacatcgt
    13561 aaaggccaat aacagcatga tgataatggc taaaatcttt gttgagccga gcgttagcat
    13621 aattctagtt ggtaaataag tctgcatcgc agctgcgatt aatccgccaa tgatcaaata
    13681 acgacctgta tcaaaaaatt cgtcgattga atgtgtcaac acatgcccag tttgatggat
    13741 cagctgattt tttggttcat tcttctcact ttgttcttct tcatggacat gatgatggtg
    13801 attttctgct actttagacg tgattttttc tttcaataca gcgtcttttt gaaaataggc
    13861 aagccagaca ccaatcacaa gggcaacaaa aaagctgcct atcacccgcc agcttgccat
    13921 ctgccaagaa ttgccaaatg cgataaaagt ggaaaaaatc acgattggat tgatgatcgg
    13981 agcagtcagc ataaaagcaa acgctgtgtg aactggtacg ccttttttaa caaattgatg
    14041 aacgatcggt acgatgccgc attcacatga aggaaagaaa aatcctaaga tcgaaccagt
    14101 aaaaatagag aagatacgat tttcaggaag ccatctcttc acacgttctg gggtcaaaaa
    14161 tacttgtaag gcgcctgaga taagacagcc taataatacg aaaggtaatg cttcaataac
    14221 gatcgataag aaaatcgtac tcatctgcaa aaccgaattt ggaataaaag aaaacatgag
    14281 tttcctcctt tttatttcta gttcattcgc tttgcaaata tacgctttta ttcaaaaaaa
    14341 aacaagttgc tctgtaaatt tttacacaac tcttattttt taccaaagca cttcaattga
    14401 ctttttctgt tattcccatt aaattaaggt ctcatacata tcacttctat cataaaaggg
    14461 agaataaaaa tgaaacaaac cactaacttg actgaagttc aagacatact gcaacagtca
    14521 tccgttgccg tcatttatct ttcgatgcct aattgcagtg tttgtcacgc tgttcgtcca
    14581 cgactagagg aacttctcac acattatgat attccggcac ttcatttgga tgcatttgaa
    14641 acaccagaag tagctagtaa atttgaagtg ctgactgcgc cagttgtcct gatttttcat
    14701 caaggaaaag aagtctttcg gcaagcacgg ttcatcgact ttaaaaaaat ccgttatcta
    14761 ctagatgaac tgacagcaga agatgattcc ctttcttatg aagaaatctt ccgaacaaaa
    14821 taatcgttgt ccaaacagaa aaaagcgatt gaacaagtga aagttttgcc ttcatttgtt
    14881 caatcgcttt ttatttttat tctgctgtat tttcggaatg cgcatttgcg tcagaatgca
    14941 agaaatagat caaggtttgc aattcattcg tcaaatcaac attttggatc aatacatctg
    15001 acggagcatt caatcgagca gcggtaaagt tcaagattcc tttgattcct gcttccgtta
    15061 actgattgac tacttcttgt gctctttcag cgggtatcgt taaaattgct acttcgattt
    15121 gttggatctt gatttgttct gacatatctt ccattggata aacggggata ccgtctacga
    15181 tgcgcccaac gatttcctca ttcacatcaa aagcacaact gatacgtatg ctattacttt
    15241 gatggaattt gtattttagt aaagcacttc ccaagttccc tacaccgatc agcgcaacat
    15301 ttgtcaattg atcgtcgttt aatgtctttg caaaaaagtg cataagatct tctacatcat
    15361 aaccataccc acgttttcct aattcgccaa aataagaaaa atctcgacgg atcgttgcac
    15421 tatctacttg aacggcttca cttaattcag ttgaagacac ttttttcttt ccagcattat
    15481 ttaaaatcct taaatagcga taatacaaag gcagtctttt agctgtcgcc tttggaataa
    15541 tatgatcttt cacgaaatga cctccacgta ttgtctaatt acatctttga tttctccatc
    15601 atagcagtat ttataggaaa aaatcaattc cattgctcag atattgtgaa attttattct
    15661 ttattcattt atttatcatt tctgcaaatc tcgcagtata atggaattta tggtagacta
    15721 ctaattgaaa aatagttttt tatttgagga gaattcacaa agtagaaaag aggattccat
    15781 gatattatta caagccaatc agattgctcg ccattttggt gcagaagtct tatttgaaaa
    15841 catacattta gaaatcagta caggcgctcg tgtcgcttta gttggcagaa atggcgctgg
    15901 aaaatcgact ttattaaaga tcattgctgg aattgaagca cctgatgaag gaaagatcgc
    15961 taaaaataaa acagcgactc tcggatacct cgctcaggac acaggacttg attccaatga
    16021 aacagtttgg aacgagatgc tgaaagcttt tgaacaagtt agacaaatgg aagagcggat
    16081 gcgcgagcta gagattttca ttagcgaagg tacgccagac acacctgctt atagttccta
    16141 tttaaaagaa tacgaccggc tgcaacacga tttttcagat cgaaacggct atggttatga
    16201 aaatgaaatt cgttccgtat tgcatgggtt taaattcgat tcttcatttt atgatcagaa
    16261 aatcgatact ttatcaggcg gacagcgcac acgtttagct ttagctcgca tgcttttgca
    16321 acaccctgac attttgattc tagacgaacc aacgaaccat ttagatatcg agacactttc
    16381 ctggcttgag aattatctgc aaagctatac tggtgcatta ttgattgttt cccatgaccg
    16441 ttatttttta gataaagtag tcaatgaagt ctatgaatta agcagaagaa aaatgcgcca
    16501 ttacaaaggt aactattcga aatatctaga tttgaaagca gaacagttag caagtgaatg
    16561 gaaggcttat gaaaagcagc aaacagaaat ccataaatta gaagacttcg ttgctcggaa
    16621 ccttgttcgc gcctctacca caaaacgagc gcaaagccgc agaaagacat tagaaaaaat
    16681 ggaccggctg gatcgtcctc aaggggatga aaaacaagca aactttctat tcagtataga
    16741 aaagccttct ggaaatgtag tcttgcaaat cgaagatgcc gctatcggat atgataatgt
    16801 caccttatct gaaccaatca atctagatat ccgtcgtcaa gaagcgattg ctctagtagg
    16861 tccgaatggt atcgggaaat cgacgttgct gaaatcaatc attcaccaga tcccttttat
    16921 ccgtggaaaa gaaacgctag ggacaaatgt caccatcggc tattacgatc aaggtcaagc
    16981 tgatctgcat acaaacaaga cgatcctagc agaattgtgg gatgagcatc ctaccactcc
    17041 agaaaaagag atccgaagtg ctttaggtgc tttcttattt tctggcgaag atgtagaaaa
    17101 accaatccct ctgctaagtg gcggtgaaaa agcacgggtt gctttagcca agttagcgat
    17161 gaacaaagaa aattttttga ttttggatga accaacaaac catttagata ttgacaacaa
    17221 agaagtatta gaaaatgcat tgatcgatta tgacggcaca ctgctttttg tttcccatga
    17281 ccgatatttc atcaatcgga tcgcgacaaa agtcgtcgaa ctctctgaaa atggcagcaa
    17341 agtctatcta ggagactatg attattattt agagaaaaag caggaagaag aagaaatcgc
    17401 cgaattgttg gcacaagatg agccagaatc tgctcctgct cccacgaaca aatctgatta
    17461 ttaccaaagc aaagaacagc aaaagcttat ccgtagcctt caacgaaaaa ttactcaggt
    17521 tgaagaagaa atggcgcgga tcgatgaatg gatcgatcag ttggaacaag aaatggtcgc
    17581 gccagaaaac ctggaagatc acgtaaaact caatgagctg aaccaagatt tagaagctgc
    17641 tagacaagat caagaaacaa aattgacgga atgggaagaa ctaagcttgg aattagaaga
    17701 atatgaaaat taacgaaaaa accaaaacag aaaccattca tgctgttttg gtttttcttt
    17761 ttttatttta tcaaacaaaa attccatatt tacctttttc accaataaga agaccaatta
    17821 ctagtcattc tcactaaagc ttctaaataa tagtaatcac cccaaataca aggtgtattt
    17881 accccattat ttttgggctt attataaaca gactgtgtta aaaagccatc ttcttcttcc
    17941 gttgtagcat atctttcttt caattgtcgc ataattttcc aaacagcaga ctggtataag
    18001 ttcttttctg ttttgtctaa tagacctgat tctattaatt ctaataatcc acaaaccatg
    18061 atcaccatac tagatgtatc tttttcttct ctgctagaat catcaaagat caaatcccaa
    18121 taacaaatgg tatcttctgg caaccgattc ataaaataat tggctaatat tttgcttaaa
    18181 tcaaaatatt tttgttcttt caaataacga taacttaatg caaaaccgta aatcccccat
    18241 gcttggcctc tagcccaaca ggaattatca ctataacctt gatgcgtact tccatatctt
    18301 ggttttccat ctttgacatc catataaaat gtatgataag ttgaaccatc ttctcttact
    18361 aaataatctg ctgcattttg aatatgagcc tgagcagctt ttttatattt ttgatttcca
    18421 gtttcttcac tcgcccaata caataatgga agattcatgc aacaatccat gatcattcta
    18481 ccttgttgtg aaggatcact aggatctccc caagcttgta taactcctgc cttctcccaa
    18541 aaacgctgaa gtaatgtttc tgcagcatca attgaagctt ccttcatttt tcgatttccc
    18601 gttattttat aatctgctac ggttgataaa gaatacaaaa aaccaagatc gtgatgatta
    18661 gtgataattt gacagtcaat cctgtggata aaatctatat ctgctttttc agctgtttgt
    18721 tgaaataaag gatcacccgt cttctcaaat aacaaccaat taattcctgt ccaaaatcct
    18781 tctgtccaac caatattgtc tcttaatgga taacgtcctt ttataggaca ctcgcttggg
    18841 aattgatact gactgttact gatacgtttg attaacctct ccgtgtgtct gcgacaacgc
    18901 tcgatctcat tattccattc tttttccatt aattctttat aacattcatt tatccatttt
    18961 tctggatgta ttatctcttc aaacatcact ttatcaagca tttccccacc agcctttagc
    19021 ataattttag tcttttttta ttgtttttat tttggatagt tagcccggtt gtcccatcaa
    19081 aatcaatttt gggacgattg ttccatactt ctaaactata atcccgatct tgatgagcaa
    19141 ccactcctgt tccgagccaa tgggttcctt ttccaaatgt accctctaat ccaggaataa
    19201 aagtgttttc ccatgtcatc aagtttgtat tgggcaaacc cataatagct gtggattttc
    19261 gtttaatagt atcgtcactt aaacagataa tacctgaaaa gccagcttca ctcaccctat
    19321 agttttcctt gtcttcttct atgattattc cttctttctc aggagaacat tcaatagcaa
    19381 accctgtctc atatagaaca tattctgtat ctaattctac tttgtgtacg cgaatctgcc
    19441 agccttcaaa aggaataagc caagttctga tttttgcctg taaagtaggt tcccatgtag
    19501 aggcaagtac ctctttctta catacaatgt ctcgtacatt atttcgaacg atgaactgtc
    19561 cataggtttt gatgctctca ttcttaaaac tggtcgtgat atatgtgtcc gctcgctgaa
    19621 tccctagcat tgaatccatt gccatttctg ctttcaattg attcgctcgc ggtatcgaaa
    19681 aaccaaattg actagaataa gcaaatttta aatatttttc attggtcaat gtatggaaat
    19741 ttggccctaa ttgtcccgca ttcaaaaaaa atgtatgtcc attatcatgt tgtatgagca
    19801 aattggcttc tgaaagaagt ttagttcctt cacgaatagg cataggcatt tctttagccg
    19861 tccaatactc atggtcttca ggtagcgcca acaataaaaa aatcttattc agccataatg
    19921 gagataacgt actattatag ggttcagtca atgtcaattg agggtaggta taaccaatat
    19981 tcaggatacc tttttcatca aatatttctt tttgcatcca ccatcttata ttccgattga
    20041 ctaacccctt gatttcacct aatttaaaag gcaaaagatc ggcccacaac atagctgacc
    20101 aaaagcttga aactgcaaaa cgataaatca tgcttcgacc aaaaggcaca tttgcaccat
    20161 cttccgcaaa aaaatgaata tagtctctag aaaaatcaat agctcggttc ttatatatct
    20221 ggctaatggg cttttctggt tctagtacgc tataaatcaa accataataa tgaaatgcaa
    20281 aaggattata ataatcttgg cgacctcttg aactgtctcg ataccagcca ttttgaagat
    20341 aacaattttc tatattatca aatgcttctt tcagtgctga ttctttaact ggataaccaa
    20401 atttttggat aattgttccg acaattatct taaaaaattg ccaattacca tctgcacatt
    20461 catattcaaa aatttgcgag aaccaatgaa taatctggct tttttctcct gtatctaatt
    20521 ttttccataa taattgttca tgatgatata acgctaataa cattgcgggc atttctacca
    20581 tacgctggtc tctttcatgt atttctcccc aataatcggg atgggaagga tctgttccag
    20641 cttttattcc tgatagaatt aatttcaacc atcgttcatc taattggtca attgcgggcc
    20701 ccgctcccca taaaacccga gagaatcctt ccattcctgc aattttattt ccatatccta
    20761 ccccttgatg tatataatca atttgtgcgc cacttgctcg ataaaacggc tggagtggtt
    20821 gatttatctt tttaaataag tcaaagtaat cctgtctggt ccttaatagt ggtactggat
    20881 gtttcattgt attttctcct ctttttacta ccaaatagct aattccgact gttctaatcg
    20941 aagtaagcct tcaataaaaa aataatcaga atagatcagt ggcacattat aagtcagttc
    21001 tctatggtat gcttctgctc cgcctccaat gatcccatct tcatccatat tccaatttgc
    21061 atagtgcttt tctgtctttt ggagtatatt gatagcagct tcatgataca tttttgatcg
    21121 tacagaggaa acatatcctg ctaattccag aagaccgcat gcagcgcata ctccagcact
    21181 ggtatcaata ttcgaatctt ttacagcaaa atcaatgaga ggaatataac cagttttttc
    21241 gattttctga ataaaattta ctgctatctt ttctgctata gctagatagt acggattttt
    21301 tgtgtaacga taacttaacg cgaatccata taaagcccat gcatttcctc tgctccaaca
    21361 agaattctca ttgtatcctt gcccgccaag taaccgaata tatttccctg attctgcatc
    21421 aaattctgcg atatgcccgg ttgatccatc tttcctcact agatacttag ctgttgtatc
    21481 tgcatgctgc cgagcaattt tagagaacct cggatcacca gtttccctcg aagcccaata
    21541 cagtaatggt aaattcatca tactatcaat aatcatccaa ccaggtttat ggtcattcca
    21601 tgctactaaa tagttccctg aaatattata gcgtccagca agtaccgtag ctgcatgtaa
    21661 ccctagttgt cgactatgtt tgttttgaga ctgttgataa tgtgcaatag ccgatggtaa
    21721 gaataaaaaa cctacatcat gatgaacgtc tgaaaaatgg tacagggcag attctaataa
    21781 ttgttcttgt tgtattgctg cctctttgaa cttttctcgt tttgtcttgt gaaagagctg
    21841 ccataaaata ccactccaaa aaccattggt ccaccagtcg attcctctgt cctttattac
    21901 gtctgtatat tttccgttgt aagaaatata tggaatttta ttccccattc tgtctaattc
    21961 cgcatctatc ttttcagaag ctacattcaa aatttttctt gaccattcct tcgtctgttc
    22021 attcatggat atcccgccat tcttttaatt tttatgaatg tggagttgct cctagaacga
    22081 actagatggt taacattcgt tatttttttt acttatttgc agctccaatg tcattctcct
    22141 taaatttaga ctgcgcccac acataaatca agaccattgg gatcactgaa gttgctaaaa
    22201 ttagcataat tgcgccataa ttaattggct cacctgtact agaaaccgtc atatttttga
    22261 taattaaggg aaccgtatat ttatctttat ctgaaagcat gactaatgga aatagatatt
    22321 gattccaact tcccataaag ataagtaaac cactagtaat aatcgtaggt gataaaatcg
    22381 gtacagcgat tttcgtaaaa atcacccact cattggcacc atcgattctg gcagcctcca
    22441 tcagtgctgt aggaaattga ctgctatatt gtcgcatcat aaacactgaa gttgtactgg
    22501 caatcgaagg gatgatcaca cctgcataag tattcgataa tcctaaacga ctcataatgt
    22561 aaaataaagg aatcaatgtg gtaaaaaaag gcaccatcct agatagcata actgtattga
    22621 acaacagttt attccccata aaacgatact ttgctaatgc ataacccgcc attgtagaac
    22681 tggctgtagc tgaaactgta ctgattacag caataacaat cgaattccaa ataatcaagg
    22741 gataattata tctcgccatg atggcttgcc agttttttaa tagcatatcc ccaaacttga
    22801 aattgattcc gccacttaaa atttcttgat tactttgcgt tgaagctaca atcatatata
    22861 aatatggcat caaaaatatt aaggcaccaa acacaataat gacgtacgta aaaattctgc
    22921 tttgtatctt ttgtttagtc atttttatca cctccgaatt taaattgaat agccgataat
    22981 ataacagata caaacaccat cgtccaggca atagcagagg cttttccaaa atcaaccatt
    23041 tcaaaacttg ttttatataa attgactcct acgaccaaag ctgaattttc tggtccattg
    23101 gaagtcccaa agaatatatt tgggatattg aaattaccca agccattgat agtggctaaa
    23161 actgtggcca ttaaaatgat tggctttagc atggggatcg ttaataaaaa gaattgccga
    23221 aatttcgatg ctccatctat ttcagcactt tcgtatatct ctttagaaat cccttgaaga
    23281 ccagctaaaa ataaaatagt gttatatcct acactaagcc aaatcatcgt gatgatcatt
    23341 gatattcgta tccaaaaagg atttgttaac cactgtacac cgttaattcc tagcgcattc
    23401 aaaaatccat tgaataaccc agtcggattg aaaaaagtca tgaatacgga agctacagcc
    23461 accgttgatg taatggttgg aatataatag acagttctaa aaaatccttt tccttttaac
    23521 tgtgctgaat tcaataaaac agcaaagtaa atagctaata gcatcgttat tggaattgat
    23581 cctgccatga taatcaaaac attcgtgtaa gatttaataa acaatggatc tcggaagata
    23641 gctagataat tgtcaaatcc aacaaattca aatgagtagc caattttcat aaaactaaaa
    23701 gcaaacgaga taatgatggg aatcaaaatc gtcacaataa agaaaataac aaaaggtgtg
    23761 accaatccat aagcactttt tttatgagac gtatctgttg cttttttctt gctgttcttt
    23821 attgtcttgc ttggtaacga agattgcatc atcgttaagt cctcctaata ttttttaatc
    23881 accagttaat tttacgccgt acttatgagc aaaattttct gcttctttta gatatgaatt
    23941 ttcaaaatca cctgtttgcc aatagttata tgcagcggta gtcaaataag acgtagcatc
    24001 tgaataagtg gctggtaaaa cagtatttga tgtattttca ttgattttat tcaatagttc
    24061 atagtatttt tgtcctcctt agtagtcaaa ctttttctca gctgcttctg tttgataggc
    24121 caatgagtta gatgtagcca cgcccagctc cagtgcgttg gctaagcatt cttcatctgt
    24181 cagcataaat gtaagaaatt gttgtgccac ttgtggattt ttggcatctt tagggacata
    24241 aaaacttgat cctcctgata ctgcaccttt tcctggattt tcttttgaaa aaggaatcag
    24301 tggagccatg cgccatttgt cagatgcatc aggaaaattc aaagacatat tcgtagcata
    24361 ccagccgcca gcaacaaaca ttgcagattc ttgaaatgct ttctcagcat cttgtgcacc
    24421 gtagaaatgt acaatatctg agtcgatcat ttctttgatt aactttgcag catgtttcgc
    24481 ttctttagta ttcagacgga tatttccttc ttcatcaagc aaagattgat tctgctgggc
    24541 tagcatgttg ataaacaatt cttggtcccc agattctggc aaggcaattc caaaactacc
    24601 agttttttct tttaatgctt ttgccacctg gatatattct tcccaagaac tgatttcttc
    24661 aaaatctaca cctatttcag caaatttttt tggttgataa taacaaagtg ctggggtaaa
    24721 atcactagcc caaggaataa tcgtttcatt actcgtttgt tgacgcaaaa tattcaattt
    24781 ttgatcataa aactgatcgc catatttgtc ataaaatcca tattcatcag cactataaaa
    24841 agcatcttga aatttttcca gcacacctgc tgctgaagtg tctgccatgg agataatatc
    24901 cggcatatct tcgtgtgtta ccaattttgg ggtgatcatt tgtactgttg ctgttcccga
    24961 agctacattc acaattttta agtcaatatt tgctccagtt ttgcggttat agatttttgc
    25021 agcattttca acatagtttg tcagtttgcc gtttgcgaca tatacttcta atgtagtggc
    25081 tttttttatc attggatctg cgatgtttgc tgttacttct tcgttagttt ttccacatgc
    25141 acttaagctg aataataggg taatgtttag caaagctatc accaatttgt atcttttttt
    25201 catgtctatc ccttctttct tttcaagttc ttatataaaa cgctttcact tatgtatatt
    25261 aacggaatta gtaacacaaa tatttatata aaaaaatatt ttacttgtcg ttttggcttt
    25321 tcatttttgt tataatagtc attaattata aatagaaagc tttttatata aaaagagaaa
    25381 ggaaagtggt tcttttgtta acaaacaacc aatcctccaa tcttgtcaaa aaaacactaa
    25441 cctattccat agatacccat cttcatttat ctgctgctcc ctattttcga ctactaataa
    25501 atgaaagcgc taatttatat gctgaatttg cacataaatc tatagaacta gataatcctt
    25561 cccttttatt cattgataaa cagcaagaag tccaaatctt ttctactcaa aaggaaacac
    25621 aaacttttat cattatccag ttttattttg atgagcaatt attagcacat attacgggac
    25681 aatctttttc tatagatcaa gaaataacat ctatcattac ttttctgaaa aatgaacagt
    25741 tcaaaattga ttatctaaaa catagtaagc cagctatttc ctatgatcag gaaatcaatt
    25801 cttttttcat actatgccaa cagatgagca ttcatttgct ttccttatta gttacttatt
    25861 gtctgcaaaa aattcaattg tcttccttac caaggcatta tcaacaggaa cttgctttat
    25921 tgacaatacc ttcaaatgag aaagtcattc ctaaaaaaaa gcatattctt tattcagatg
    25981 aattagttga tccaatcata cgatatttag aagaacatct ttcagaaaat tttttgcttg
    26041 aacaattagc ccaaattttt tttatcagtc cagccagctt aaaaaaacat tttagaaaag
    26101 tgaatggaaa aagtgttatt agctatttta aagaactaaa aatggaaaaa gcaaaacgtc
    26161 tcgtgctaga aaatgaatta acctatacag aaattgcaga tatattagga tttcattcga
    26221 tccatcattt ttcttcagct tttaaaaaat atacagggtt atccccttca aagtattatc
    26281 tatccttaaa atagaaaaaa atttttaggt tttatccaca aattatttct ctatattttc
    26341 tgtctattca aaataaacaa taattttccg gcacaattat ttgctgtttt ttaccataac
    26401 atagtacgat caacgtagaa aaggagatgt ttttatgaaa gttgttgtca ttggcggtgt
    26461 agctggcggt ccttcatttg ccactcgttt ccgtcgattg aatgaagcgc acgaaatcat
    26521 tatctatgaa cgcggagaaa atatttctta tgcaagttgt gctttgcctt attatttagg
    26581 aggtgtgatc actgaccgtg actcgctgat cgaacgtaca ccagaaatat tgaaaacaaa
    26641 aaacaacatc gatgtcttta ctaaacacga agtaacagca atcgatcctt ctactaagcg
    26701 attaacagtt aaagacctat ccacaaatga agaaataaaa acagattacg ataagttgat
    26761 catctcttct ggtgctagac cagattatcc ggatattccc ggagtttttg aagcagaaaa
    26821 cggctttgtc ctccgtagtg tgacggatgc ggatcgaatc aaatcgttcc ttgaagaaaa
    26881 aaatccacaa catgtcgtca ttcttggtgc aggtgttatg ggtctggaat tagctgagaa
    26941 tttcaagcat cgcggcttag acgtgacttt aatcgatcaa ttgccacaag tcgctttccc
    27001 ttatgatcca gaaattgcta atttagttta tgacaaattg ctgaaagaag gattagccgt
    27061 tcatttagaa acaagagtta ctgagatccg tgataaaggt cgagaaatag tattatcaga
    27121 tggctccgtc ctttctgctg atatgctaat ttttgctgtt ggtgtttctc cgaagaatga
    27181 agtggtgaaa gcaaccggca tacaattatc agatacaggt cagatcattg ttgatgacca
    27241 gttacaaacc aatctgccgg acatctatgc gattggcgat attatcgaaa cgactagtgt
    27301 agtgactggt cagccgatcc aaagtatgct ttccagtgcg gccaatcgtc aaggacatat
    27361 gctggcagat attttaaatg gtacgcctat gcgctatcgc ggatatattg gtgcaggtgt
    27421 cgcaaaaatc tttgatcata cagcaagtta tgcgggaatg acagaacatg aattaaaagc
    27481 atcaggcata acaaattata aaactgtttt tatcactccc tttgaccatg cctatttcta
    27541 tccagaagct acaagattaa atctaaagct gatttttgat gcagatagcg gtcgtatttt
    27601 aggtggacaa gcatttggag aaaaaggtgt cgataaacgg atgggagaac tttctgtagc
    27661 aatcaccgga aacttgacag tctttgattt gcctgatttg gagttgcctt actctccacc
    27721 gtattctact acccgtgacc cgttgaatat agctggttat gtcgcaatca atcaaatgac
    27781 gaatatcgta gaaacgatca aagcaagtga tatacccgaa aacgatttga aagaagcgtt
    27841 ctttttagat atacgtgaac ctaataaagc accttctggc agtatctcgg caacaaaaaa
    27901 tattccaatg aatgagttac gagatcgaat caatgaaatc ccaaaagata aaaaagttta
    27961 cattactttc agaagaggat tgaatactta tacttctgcc cgaatcttgg caggtttggg
    28021 tatcaaagcg attttgattg aagaataaaa aaacgaccgc ggtgggtagc cgcggtctag
    28081 gagttatttt tacttgagga gtaaaaatga aaaagtatat agggttgttg gttggtatgt
    28141 atatagaata gaagataaat gtgaagaaat tgtgcattat ttcttcacat ttattaggtt
    28201 atagtttaag aaagtttaaa cattgcgcaa tttaattatt ttagctgttt cttttcataa
    28261 aaaaacatgt tgtgactctg agacaaatca tagtccatag tgatgtctct agagtaacta
    28321 cgcctaatat tacgaggcat catattaatc tatcagcact agtacctaaa ctagcatccc
    28381 tttcgaatat tttttcttat ataataaagt ttgctttcac tcctctaatc tcaccaaatc
    28441 gactggcttg cgttgtgtga tactaggaac tgattggttt agaacgactt ttctattgta
    28501 atcatatttt tcgccatcct cgttgtcttc taatccttca atgaatgatc cctaatgtgt
    28561 gtattaaata atttaatttt tttgttccta tttatttaa
//
LOCUS       DAFEOM010000028        27599 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.029, whole genome shotgun sequence.
ACCESSION   DAFEOM010000028
VERSION     DAFEOM010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.029"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            84..731
                     /locus_tag="LP524_001431"
     CDS             84..731
                     /locus_tag="LP524_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBL2234492.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            815..1759
                     /locus_tag="LP524_001432"
     CDS             815..1759
                     /locus_tag="LP524_001432"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HBL2234493.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1894..3345
                     /locus_tag="LP524_001433"
     CDS             1894..3345
                     /locus_tag="LP524_001433"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HBL2234494.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3366..4202
                     /locus_tag="LP524_001434"
     CDS             3366..4202
                     /locus_tag="LP524_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBL2234495.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4202..4861
                     /locus_tag="LP524_001435"
     CDS             4202..4861
                     /locus_tag="LP524_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBL2234496.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            4987..5676
                     /locus_tag="LP524_001436"
     CDS             4987..5676
                     /locus_tag="LP524_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234497.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5682..6638
                     /locus_tag="LP524_001437"
     CDS             5682..6638
                     /locus_tag="LP524_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HBL2234498.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6682..7194
                     /locus_tag="LP524_001438"
     CDS             6682..7194
                     /locus_tag="LP524_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2234499.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7450..7998)
                     /locus_tag="LP524_001439"
     CDS             complement(7450..7998)
                     /locus_tag="LP524_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL2234500.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8087..9268)
                     /locus_tag="LP524_001440"
     CDS             complement(8087..9268)
                     /locus_tag="LP524_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBL2234501.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(9504..11012)
                     /locus_tag="LP524_001441"
     CDS             complement(9504..11012)
                     /locus_tag="LP524_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBL2234502.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            11171..11362
                     /locus_tag="LP524_001442"
     CDS             11171..11362
                     /locus_tag="LP524_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234503.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            11565..12482
                     /locus_tag="LP524_001443"
     CDS             11565..12482
                     /locus_tag="LP524_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HBL2234504.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            12475..13548
                     /locus_tag="LP524_001444"
     CDS             12475..13548
                     /locus_tag="LP524_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234505.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            13696..14214
                     /locus_tag="LP524_001445"
     CDS             13696..14214
                     /locus_tag="LP524_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234506.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            14195..14587
                     /locus_tag="LP524_001446"
     CDS             14195..14587
                     /locus_tag="LP524_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HBL2234507.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            14663..15598
                     /locus_tag="LP524_001447"
     CDS             14663..15598
                     /locus_tag="LP524_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HBL2234508.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            15731..16510
                     /locus_tag="LP524_001448"
     CDS             15731..16510
                     /locus_tag="LP524_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBL2234509.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            16503..16820
                     /locus_tag="LP524_001449"
     CDS             16503..16820
                     /locus_tag="LP524_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBL2234510.1"
                     /translation="MTKLYLLLLVVCLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            17064..18674
                     /locus_tag="LP524_001450"
     CDS             17064..18674
                     /locus_tag="LP524_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL2234511.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            18831..19679
                     /locus_tag="LP524_001451"
     CDS             18831..19679
                     /locus_tag="LP524_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBL2234512.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            19910..20389
                     /locus_tag="LP524_001452"
     CDS             19910..20389
                     /locus_tag="LP524_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234513.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            20422..22650
                     /locus_tag="LP524_001453"
     CDS             20422..22650
                     /locus_tag="LP524_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL2234514.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            22779..24422
                     /locus_tag="LP524_001454"
     CDS             22779..24422
                     /locus_tag="LP524_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBL2234515.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(24495..25040)
                     /locus_tag="LP524_001455"
     CDS             complement(24495..25040)
                     /locus_tag="LP524_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HBL2234516.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(25063..25548)
                     /locus_tag="LP524_001456"
     CDS             complement(25063..25548)
                     /locus_tag="LP524_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL2234517.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(25538..26350)
                     /locus_tag="LP524_001457"
     CDS             complement(25538..26350)
                     /locus_tag="LP524_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HBL2234518.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(26489..>27599)
                     /locus_tag="LP524_001458"
     CDS             complement(26489..>27599)
                     /locus_tag="LP524_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBL2234519.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         9040 a         5024 c         5523 g         8012 t
ORIGIN      
        1 tttaattttt aaacatgaca gaaaattttt tcttcgttat acttgtaata gaatactatt
       61 ttataacaaa ggggatcgaa tcaatgaaaa aaactgttat ttatactagt ttatttgcag
      121 ctttgttttt agctgcttgt caatcaaata ccaatcaagg cacatctggg aatacgtctt
      181 ccagtagcga acagcgctct tcacaggtat cggactcctc aacaaaagca agcacatctt
      241 cgtctagcca gacaaaaagt tctagtgaag caagtactca gtcagatatc aaagtgtctg
      301 cagctcgtgc gatcgaattg ttccaagata aatacccaga tactgcaatt actagtttgg
      361 aattagatag tgattgggga tcttacttct acaagataga aggtgtcgat gatcaaaaag
      421 aatacgaagc aagaattgat gctcaaaatg aaaaaatgga gacagagaat ccagaaactc
      481 tagatcgaga tgaacaaaat ggtcaaaaga aaaaagaaga tggtctggat gttgataaat
      541 tgatcacgat tgaggaagca gcaaaaatcg ccgtcgacaa agtaggaagc ggtacggcaa
      601 cagattggga tttggataaa gatttaggta ctacttattg ggaagtcaaa gtaaaagacg
      661 gcaatcaaag tacatcagtc aagatcaata gccaaacagg cgaagtactg tcaactgaac
      721 aagatgatta agaatttctt cggaaaacaa gagttgattt ttcttttagt catgatacac
      781 tagacaaaat aacttgtttg aaggagcgat ttacttgagt ttagaaacga cctttgccaa
      841 tcatatcttt gccaatcctt tgatgaatgc atctggtgtc cactgcatga ctacccagga
      901 attagatgaa ctggctcatt cagaagcagg agcatttatc actaagagct gtacgatcaa
      961 tgaacgaaaa ggaaatcccg aaccacgcta ttttgatgtt cctttaggga gtatcaattc
     1021 tatgggactt ccaaacttag gtttttctta ttatctagag tatgctcttg cgtatgaaaa
     1081 agtgcaggaa aaccaaaatc aaccgttatt tttttccatt gccgggatga gtgtccagga
     1141 aaacttggaa atgcttgaga aaatagaaaa aagtgggttt aatgggatca ctgaattaaa
     1201 tttatcttgt cccaatgtgc ctggaaaacc acaactagct tacgattttg aggccactta
     1261 tgaaacactg aaagaagtat tcagcatctt ttctaagccg ctaggaatca aattgcctcc
     1321 ttattttgat tttgctcatt ttgatcagat ggctgatatc ttgaatcagt ttccattgac
     1381 ctatgtcaat gctatcaaca gtgtgggcaa tggtttgtat atcgatacag aacaagaagc
     1441 agtagtcatc aaaccaaaag aagggttcgg cggcattggc ggagagtata tcaagccgac
     1501 tgctttggcg aatgttcgcg ctttttatac acgtttgaaa ccagaaatcc aaatcattgg
     1561 tacaggtggg attcggacag gacaagatgc atttgaacat ctgctatgtg gcgcaagcat
     1621 gttacagatt ggtaccgagc ttcataaaga agggccggaa attttttcta gaatcataaa
     1681 agaattaacg cagattatgt cagaaaaagg atatacgtca attgacgaat ttaaaggcaa
     1741 attacgcacc atttcatagc aaagagacga aggtcatgat tgatcttcgt ctttttttat
     1801 aaaaaacact cgtctgttca ttcctgtgca agcgtactca ttgtatgtta gacgaacttg
     1861 tgggaaaata acagaggcaa aaaggaggat aatatgaatg atgcaacaga attagcaaaa
     1921 gagcttaaag aaaaagaatt tagttttcaa gaactagtta cggatataca gaaaaaaata
     1981 aagaagcaaa atcatgaatt gaatgcgttt gtcgcgcttg aaccattcga gagtaattcc
     2041 cacgagctat tgattcccca gtcagaaaaa gggccattgg ctggtatccc atttccttta
     2101 aaaatgctcg gtcaagaaaa aaagggatgg ctagctactt caggatctcg tttatttgaa
     2161 acacaccgag cttctcatac atctaattat gtgcggcaag cagaagcgat aggtctagtt
     2221 ccttttggac aaaccaatgc gccagagttt ggtttcaaaa atattacaga cccagtcatc
     2281 tatggcccag caagaaaccc ttggaacctt gaatgtacac cgggcggatc aagtggagga
     2341 gctgctgctg cagtttcatc aggaatcgtt cctttagctg gtgctagtga tggcggtggt
     2401 tctattagaa tacccgcttc gttttgcgga ttgattgggc tgaaaccttc gcgagggacg
     2461 atgccagttg gtccatatgc ttggagagga tggcaaggag ctgctattga tttcggcttg
     2521 acagtttcta tgagagacac ggaggcttta ttttatggga tgagaagtat ccattccggt
     2581 gcgccatatc aagtatcgcc agttgagtgg caaacacatc caagaaaaaa acggttgaag
     2641 atagcttttt gtgttgattc accgatccac tcacagatta gtgaagaggc agtacttgct
     2701 gtaaaaaaag ctgtagattt ccttggaaaa gaagggcatg atttgacaga gattccttac
     2761 ccactagatg gtcgtaaact gatcacgtct tattatcaga tgaatggcgc agaaacagca
     2821 gcaatgatcc gctcgattga aacaggcatc cagcgagctg tcagaaaaga agaacttgaa
     2881 cccatttctt ggactttgtt ccaatatggt cagaagctct ctgccgctac ttacatcgat
     2941 tctctgcatg tatgggatga agcagcaatt gtcatggaaa acctacatca agaatacgat
     3001 ctattccttt caccaacgac tgcatttacc gcccctaaaa tcagcgaaga tttgcaaagt
     3061 gatgcgataa gagcacagat ggaacacatc agtgattgtt ctgaagtaga aggtttagac
     3121 gttgtttctt ccatgtttga ggagagcttg aagatcacac cttatacaca gttggctaat
     3181 ctgactggac aaccagcaat cagcttgcct actcatttga caaaagaaaa catgcctcta
     3241 ggaatccaat ttatggctgc cagaggaaga gaagatttgc tatttcaaat cggctatcta
     3301 tttgaagcaa atcatcagtt ccatttacca aatccatgga aatagcagaa acaaggagaa
     3361 gaagaatgaa gaaaaaaaga ttgtttgctc taataactag tgcctttttt attttaagcg
     3421 cttgcggtac agctgccgct aatcaggaag ataacagctg gagtaatgta gaaaaaaaga
     3481 aagaattagt agtagcgact tctggcacac tgtttcccgc atcttattat aatgatcaaa
     3541 atgaattagt tggttatgat gtagatttgg caaaagaggt ggctaaacgg ttaaatgtca
     3601 aaatctcctt taaagaatac aacgtggatg gacaaatcac ttctgtttca aaaagagaat
     3661 cagattttgc tgcgaacgat tttggtatca caaaagaaag agaaaaaaaa ttctcccttt
     3721 cagagacttt caaatattca tttgacagta tgatcgtacg aaaaaaagat gattcaggaa
     3781 tccactcact tgaagatctt aaagggaaaa aagcagctgg cgaacccaat actagctata
     3841 tgcgtttagc ggaaaaacac ggagctaaat tagtcacata tgacaatgcc acgaatgatc
     3901 agtatttaag cgatgtagca aatggtcgaa cagatgttat tctaaatgat tattatctgc
     3961 agaaaatggc cgtatctgct ttaccagata tccctgtaaa aatactggag aacgtttatt
     4021 tcaatgaaaa cgaaagtggt tttttattga aagaaggaaa tgatgctctg aaaaagaaaa
     4081 tcgatggcgt attaaaagag atgaaagaag atgggacgac aaagaaaata tctgaaaaat
     4141 attttcaaac tgatgtttca gtcaaaccga aagaaacgat ggctgagtcg gtgagtgagt
     4201 gatgtacttt cctctaattg atttttccct catgtggact tcacttcctt tcgtactgca
     4261 aggcttggta tacacattag ggattggctt cgtgtcgttt cttttaggaa atctggtagg
     4321 gttgcttttg acagttttag gtcttttgga ttggctgcct ctgaatgtat ttattcgttt
     4381 ttatttatcg ttctttagag gcattcccgc actagtcctg ctgtttctgt tatattttgg
     4441 gttaccttat caattaagtg ccttaacggc ttcagtcatc tgctttacga ttaccagcag
     4501 tgcatttatt ggtgaaatct atcgaggatc actagcaggc gtttcttcag gtcagtggga
     4561 cgctggttta gcttctggtt tttctttctt tggggtattg aaatatatcg ttttgccgca
     4621 agcttttcga atctcagtac cggcactcag taacgtagct atggatctag tgaaaggtac
     4681 ttcgttagca gcaacaatca cagtgccaga aatatttcaa aaagcaaaga tcataggcgg
     4741 tcggacattc gattattttt ctctctatat tttagttgca ctgatttatt gggtcatttg
     4801 tatcgggatc gaaaaagtgc aacagtattt agaaaaacgt tctatgcatc agtcaatctg
     4861 aaaaatagaa atggtgctag cgtggctggc accatttcta ttttttgttt cttgtatatt
     4921 ctgcctagta ttttaaaacc atttcatgta aaatgtaaat cgaaaatatg ttcggaggta
     4981 gaaaacatga agaccccgct aaccatcgaa atagaatcct cgttatacca atattgcatc
     5041 gagcaaggcg gcttggttgt agaagaagtc acgatgcctg atgaccaagg aattgtagat
     5101 acgcttagct gcttgacaaa atttgatgga agccgagaat ggcgatgcta tgagctgaaa
     5161 gtgacaaaag cagattttcg ctctacagcc aaactgtcat ttgttggaca ttataattat
     5221 tttgttttac caagtaaact atatgaagaa gtaaaaactg aaatccctac aggaataggt
     5281 gttttagtgt atcgtccgta cacggttgaa gaggagatgc ctgcctctgg tacatttctg
     5341 attgcgaaaa aagcagcaaa gaaagagctt gctgtaccgg aagaagcttt gacaaaccgc
     5401 ttcatggctt ctttattcag ggaagtaaga aaagcaaagc aaatggagcg aggcgtaaaa
     5461 tattttccaa cagatcaatt gtacaaagaa ttgaaaagac gttcagaaaa tcaagatcca
     5521 ttttctaacg aacgatatta tgaacggttt ttagaggaaa tagctagtca ggcaatcaat
     5581 gagttgcaag aagaagttat cgcgcttcag catgattatg aatatttgag gcaacaacag
     5641 cagatccgtc ggttgcctac agagccattg gagtgaaaaa tatgtactat ccttatttaa
     5701 gaggaaaaca atttgaccta ttagcattaa aagaagcact gtcgcgagga ttgctctcca
     5761 ataaaatcca accagtgatc gaaccagtac gtgattccgc tacgcttaaa aatgtgatcg
     5821 aattatttca aaaaaagcac catcgaatcg ctgtgatcca aaatccccaa gtaggacaat
     5881 tcaaattatt cgatcagcat gtgcacagtt ggacagtcaa agaaaatagc tctgtcgtcc
     5941 ctgcacagat cttaacgcca gaaaatctca gtgaagtact ggattcacct ccagcgtttc
     6001 ttgttttcga tggacagcac tacccaaaag atacagaggt gtggaaacag ttggcaggag
     6061 ttgattcgaa atttttgata ccagatacta gtcgctttcg catctggctg cccgaaaaca
     6121 aaattgtgat ccgtgactct tttcaaacga gaaagcatgt tgaaagttat gcagataaaa
     6181 cagatgactt ttttagtgat gactatcttt ttttccacgc agacggatat attggatttt
     6241 ctgatttcac gatcgaaggt agccgttatt tcgataaagg gttccctagc cgagcgcttg
     6301 ctatccatct aacgtatatt gatgcttacg gcaatatacg cgtgaaacat tttgtctctg
     6361 acagtaacga ctctgctaaa gaccaagcat tgaagtttct tgaagcagga gataaaatga
     6421 aaaaatggat catgcgtcac catcatcaac tcttgattac ttcaggaatg attgaattat
     6481 tggctcttta ccagcaacaa aaattccctg gactgggagt gttgaaaaag tggtcactta
     6541 tgcatcattt ggaacttatt agtcacctgc tagatcatcc aaaagattgg cttaaatatt
     6601 attcaaaagt tcaggaacta tatgaagtga tccaatgatt agataatgta aatgaaaaag
     6661 gaatagaaaa gaggaaactg gatgtatatt cggcaaacaa ggaaagaaga agtagaagaa
     6721 gtggtagaaa tcattaatgc cgcagcggat ttcattgctt cgcaaggctc tcctcaatgg
     6781 caaaacgggc agaaaccaac aaaagaaacg attttgaacg atattgaaag aaaagaaagc
     6841 tatgtattaa tagtggaaga tcaaattgct ggaacagctg cattagtctc aagcgtggat
     6901 cccgtttaca caaaaataga tggtagttgg aaagaatcaa ctggtcctta tttatcgatt
     6961 catcgtgtcg ctttagcaga aagtactcga ggaaaaaaac tagcaaaacc gttcttagaa
     7021 catttgttga cgattgcagc cagcaaaaat atccaagata tccgtatcga tacttatcca
     7081 gctaatgaac cgatggaaaa gaccatctat tctgctggat tcacttatca agggatgatt
     7141 gaatttcctt ttgaacacgg cgaaagaaaa gcatatcaaa taatcatcaa ttgactataa
     7201 aatcatctga gcaaagataa agacattaac actactgaaa aaaacaatca tatgatagcc
     7261 aagaggttgt aacagaagtg gccagcttca aaaaacaaga aagaatttcc gaaaatgctt
     7321 ctcaaatttt tgtggaattt tagcgtattt tacgaaatag ctgctttctg aaactccctt
     7381 catcgagagg tcgtgacata gcttttgtca cgacctctcg gcttatccta cgagtattat
     7441 tcgatacatt tatatcaata atcctttttt tattttatct gcagcttctt ggaacgctcc
     7501 atcgtttgac acgactaaaa gtgaggggat agctttcaaa cgtttcaact gtgctaaatc
     7561 ttctacataa accgccagtc tggctgaaat ctgttcagat gaatctccac gttttttcag
     7621 gcgcgattgc aatgtttctt tagagatcgt aataaagata ggacggacta ccgtcccgaa
     7681 atactcatat aaatgccaaa aaccatttgc tgtcaaaacg gcgtaacaat ttcctactgt
     7741 cagtttcttt ttaatttctg ctttactgga accatattta tgttgagcat attggtccca
     7801 ctctgcaaac tcatttccct tgatcatctg attgaatgta ttgtcagaaa caaaataata
     7861 atcctcttga tctttttccc caggtcttgg tgatcgattc gtgtaagtaa tgatcttttg
     7921 ttccggctga aaaatagctt ctgcgagtga agtttttcca gaaccagaag gaccgataaa
     7981 tacatagcaa taattcattg tcatttctcc tttgatgatt gaacaagtga aaaagctttg
     8041 gaaaatattt ccaaagcttt taaaagtaac tttgcttttt tgtatattat accagcaagc
     8101 cagtcacaat agcacttaaa aagctgacta aagtcgctgt tagcaaaatt ttcaatccgt
     8161 gttttgcgac aagatttcct ttttcttcat ttaatccctt catcgcacca gaaatgatcc
     8221 cgatagaaga gaagttggca aatgaaacga ggaagacaga tataattgct gttgtacgct
     8281 cagtaaaatg gaaattacca tttgctaatt ctgtcatagc aacaaattca tttgtaacca
     8341 gtttagtcgc catgatacta ccggcatcca ctgcttcttt ccatgggatt ccgctgatga
     8401 aggcaagagg ggcaaagatg taacctagga tctcttggaa agtgatgccg aatatcccgt
     8461 ggaagatagc attgatcatg gcaatcaaag caacaaaacc gataagcata gcagcaaccg
     8521 tgatcgccac atggaaacca tcaagaatgt attctcccaa catttggaaa aatgtctgtt
     8581 ctttgttttc ttcaatgatc agctcatctt ctttttcttc taatacatag ggatttacaa
     8641 ttgaagccag aatataaccg ccaaataagt tcaaaacaag ggcagtgatc acatatttag
     8701 aatcgatcag tgccatataa gatccgacga tcgacataga aactgtagac atagccgata
     8761 cactcatggt gaacaaacgt ttctcagata ggaagggtaa ttcctttttt atggaaataa
     8821 aaacttccga ttgtcctaaa atagcagaag ctactccatt ataagattcc aatttgccca
     8881 tcccgttgat tttacttaat cctagaccaa cagctttcat aaataatgga agaatcttga
     8941 tatagcgtag aataccgata atagcagaaa taaatacaat tggcatcaat acggaaagaa
     9001 agaaaggtgc acttccttta tttgctacac cggcaaaaac aaagtcaaca ccttgcccag
     9061 caaaagcaag aagatgatca aacatttttg ccaatacaga aacaaccgct gtaccaaaag
     9121 ttgtacgtaa taatacgaaa ccaagaacaa gctgtaaaac gaacatcaca attagtggtt
     9181 tcaatttgat tgcttttttg ttactgctga tcaaaaaagc aagccctaat gtgacaaata
     9241 aaccaataat acctaataaa taactcaaat aatcactcct attccaaatt ccgttttatt
     9301 ttacctgttt tgcaaataaa atactaatct ttagtgaaat tttcattact tttttagagt
     9361 gaaacgcatt cattttccat tagaaaataa agcgaacaaa ataaaaggtt taaaaagtgt
     9421 aacgtcttag taaagtctat aaaaaaacga aactatcgat taagctaata gtttcgtttt
     9481 caacatttat aagtaaactt tttctattgg taatatagat tttcggaagg gtagacaggt
     9541 gcgcaggtaa tatcgattcc tagttttttc aatgtttgtt catcattttt attgaccatc
     9601 acagtagagt gggcttgtgc tccctcaagt tcagctagtt tatcataagc aacttgagcg
     9661 attggattcg ttactgcact gattgataaa gcgatcaaga cttcattgat gcttagtgca
     9721 tccaaagagg aatgaagtgt ctttgatttc aatccttgta ttgtttcaag aactaatggt
     9781 gacaataaag gaatgtcatc tgcgatatgg gctaaatgtt ttactgcatt taagataact
     9841 gccgctgacg catccatcaa tgaacttgta cgcccggtaa tgatgacttc atctggtaat
     9901 tcgattgcaa tcacagcaga aggatctttt gtaggacttt gcgcttgaat cttatctgca
     9961 tagtcatggg cagggactac cacgtttcgg tcttcaggac gtaaaccgac ttcttccata
    10021 atcagtttga ttcgattaac tgcttcttcg tcgatcaatc cttttttgaa gtcgcattct
    10081 gtttggaaat aacgacggat gatttcttgt ttagaggctt cttggacaac ggtatcatcc
    10141 acgatcccaa atcctacacg attcacaccc atgtcagtag gagattgata aacagattct
    10201 ttaccagtaa ttttttcaat aatacgtttg attacaggaa atgtctcaac atcccgatta
    10261 tagttcacag ctactttatt gtaggcatca aagtggaagg aatcgatcat attgacatct
    10321 tttagatcga ctgtggctgc ttcataagca atgttcagcg gatgtttcaa cggaacattc
    10381 catactggga aagtttcgaa cttggagtaa ccagctgcat gtcctttttg gctttcatga
    10441 tacagttggt tcaaggaagt agcgagcttt ccacttcctg ggccaggagc tgtcactaca
    10501 acgattggtt tagttgtttc aatatactca ttttttccaa aaccatcttc tgagacgatt
    10561 ttttctacgt tgactggata gtcttcgatt tctgagtgtt tatagacctt gatgtttcgt
    10621 ctttctagtt tattgataaa gactttcgtg gcaggctgtc cactatagcg agtgataacg
    10681 acactgttga ttaataagcc ataaccacgt aattcatcga ttaaccgcaa gatatccata
    10741 tcataagtaa tcccataatc accacggatc ttattacgtt caatatctcc agcatagacg
    10801 caaatcagta tttcagcttg atctttcaat ttttgcagca atttgatttt cgcatcttct
    10861 tcaaatcctg gaagtacacg tttcgcatgc ttgtcgtcaa ctagcttccc accaaattct
    10921 aaatataatt tgtcatagtg gttcactcgt tccaaaatat acgctgactg ctcttcaata
    10981 tatttttctg aatcaaaacc aatttttttc actaggttcc ctccttaagt actaaagcat
    11041 tttttcattt ttttgaggat tcgtcaactg gaagagcgaa attcctcaaa aaaacaaaga
    11101 aaatggttta aaccaacttg tctagatagt tattcattgg tatgatagtt tttgataagg
    11161 aaggtgctac atgaaaaaaa gacagaaaaa gaaaaacgca tataaacact atatccgttc
    11221 gatttttacc ggttatgaaa gaatgcttga agatcccgaa ttggaacaat tgacgtttac
    11281 ttatctaaac gaagaaacgc agctaactcg tgatgagcat cagcgcattc atttcacaac
    11341 aagagatctg cctagtaaat aaaaaataag ataaaggtca tcggattcga ttctgtccta
    11401 tcagatggaa agactagttg atcgaaaaaa aggctgtacc aaattttggt gcagcctgtt
    11461 ttatggagca ttgcccgctc ttttttataa caatttttta cagaaaatgg tactattgaa
    11521 gtattaataa gtgaaaaaca gatgagtaag aggaggcaag tagtatgaaa gagccagata
    11581 caaatgcaaa ttatgagcca gacgaactag aggagacgaa tgcggatcag gatacaggac
    11641 aagaatcgtc ttctttttac gatatcaaag aaaaagcaag catgaatctt tcccaattat
    11701 tagaagactt acaggctttt gaaaaagctg tccgcgaaga agatatccct gagatctacc
    11761 gtttatacaa tggccgattg aatgaagaat taaaaagaag ctcaaatgaa aatcacgaga
    11821 tcgatcaatt gctgatgcgt aagatccatg atcgtttttt acaggaattt ccttttatgg
    11881 aacaagtaga aaacatctcg ccaacgatga gctattacaa aatcggcact tattaccatg
    11941 atcggcccac ggtcggtatt gatgcaagtc tgccagaaat ttttgttttg cctaagatcg
    12001 atgaagagtg ggagaaatat tcccaaccgg agtcaacgga ctatgacaaa aaaatcaatg
    12061 agctagacgc aaaggtgatc actgctcaga cagaaattga acgtttagat gaacaaataa
    12121 aagaaatcaa tcgacaaatg actgatttga acgacaataa aggattcttg aatcgtaaaa
    12181 aaatcgagga agaaattcaa gaattagaga agaagaagca agtactatct aatgaaaaat
    12241 taggctggct tccttatatt gagacgcctg aaacaatcca gcagcaaaaa gaagcattga
    12301 agcaagaagc aagagcagac cagcttcgtg cagccatcgt agaaaaagaa caaaggcaaa
    12361 tcagacgcta tttcggtagc aaagagggat tcggtcaagc aatccatgaa ttcttgatga
    12421 attatcttgg caatgagaat cctaataatc aatctaatga aggaggttcc gagtatgaat
    12481 gaacacgatg aagcagaatg gacaaaaaat gaagaacaaa cagaagatca tgaagaaacg
    12541 aacacttcat tggaagaaca aacaagtaat cctctggatg acacagaaga aatccctatg
    12601 ctagaggaaa caaaaataca cgaaaaagaa aaagtaactc cgacgaatac gaatactgca
    12661 gaaaacaccc atgcttacga aataactgaa aatcccgggt atttagaagg aaaagagcaa
    12721 gatcatcaag atatcgtcag tttgattgaa acagcggaag cagaattagc ggatttacgt
    12781 atgagaaaag atttgatcga atcagatttt ccacaattat tagaatcata tgatcgtttt
    12841 atattgaatg aagaaccaat ctccacgatc ggaaagacaa gcctgatcga atacttcacg
    12901 tatgaattac tgaaaccgtt gaatgatatt ggtctggaac ggtcaaattc agcttatgac
    12961 acatggaaaa caagacattt ttcgatcaat tatacgttga atggacaaaa cgaattagtc
    13021 ttttcctttg tattaccgac catcgatcaa cgataccaag tggaatcgat gagtctccta
    13081 aacgtatcac cagagacaat ggaaatcaat gtccaagatg accgggtgct ttctttgatc
    13141 cgacactggt cagtggatcg agtattctct gcagggcaga tcacgatatt caatcacaaa
    13201 ttgaatcaat tattagccca tgcaagaaca cttggtttta ccgttaatca gacattacta
    13261 gacaatacga agccacttcg tttgaatctt cagagcgaat ttgaattgac gaatgaagta
    13321 ttggatgata tttttatcgt ggcaatgaaa aatccatcct acgatatgga aaagatcaac
    13381 gaaactactt atcaagtacg attagataaa ggacagtctt taacgataac aacaaacgaa
    13441 aaagatcaaa cagtcttgtc gatctcatct ggagactacc cacgatctgt cattgatttc
    13501 tttatcaatt acgaattctt ggtcccatta atggtgagaa agtcctgatt ggataagttg
    13561 gattaccaga tgtcattcag ataattctgt tccaaaggac ttgcggcagt tatccagaat
    13621 aattgtcaca agtcttttct gtatgtacta aatggacgtg aataacttgc tgatgaagga
    13681 gggatttcat taggaatgtc cacaaataaa atagcacagc ttagcttatt gtctgctgct
    13741 tgtgtagcag gtaggatcgc ttttcaattt attccgaatt tccagccaat gactgctatt
    13801 tttttattta tagtacttta cttaagctta aaagatgcgt taattgtcat gagtctttct
    13861 attgcgatca gcagttttta ttttggtgtt ggaccatggg tcatcggcca atggatcagc
    13921 tatactgttg ttctgagttt gttttcaatc gtctgcttga gaaaaacagt gcaaaggaat
    13981 ctatggttaa agggagtctg tttttttctg gcgggcatgg tttacggaat aggtatgacc
    14041 gtttttgata cgctgttata tcgtttgcct caaccttgga tctattacct tcaaggggtg
    14101 tcgtttgatt tgatgcacag tataggcaat gttgtgttct ttcttgtttt tctgccagtc
    14161 gtcaaacgtt tttacaatca ttcaggagga aaaaatgaaa aaaataattt atagtttgct
    14221 tgcaatgagt gtcagtatac tcattttgac aagttgcagt acaacctata cacaatcggc
    14281 ttccaaccaa aaggaggaag taaaaacatc gcaaaaagca acaatcacgt tacaagaaaa
    14341 tggtcaaaac ttttcggaaa aagaagtgtt cttcaaaaaa ggcgatgatt tactgagtat
    14401 tttgaaacga aattttgaag tcaaagaaga taacggcttc atcacttcgc ttgaagggca
    14461 ctcacaggat gagaaaaatc aactctattg gatgtttact gtggacggga aaagtgctac
    14521 tactggtgcc aaagagatca aattgaatga tggacaatcg gtcattttca atttgtctaa
    14581 actataagtt tcataaaaaa ctgttaaagg gcgaatattg ccataattca gtgagttcag
    14641 ttgatgaaag aaggaaaata acatggaaaa ttttaaaaaa agcggaatgt tttccgtaat
    14701 tgctgctcta ggggctaata ttctagttgc tatcagcaaa tttgtcggct atgcaatcag
    14761 tggcagtgcc gccatgctaa acgaaagcat ccacagtatc gttgattgca gcaatcagat
    14821 tttgctgtta ttcggtgata aacaagccaa taaaggacag agcgagttgc atcaatttgg
    14881 tgaaggacga gcaaaatact ttttcagtac aatcgtcgca atgatgcttt tcttcggcgg
    14941 tggggcactt ggggtgatgg aagcttttga aaagttgacg catccatccc atgaagtggg
    15001 taatccgatc attgttatag caatactgtt atttggtttg gtaatcgaag gaagttcgtt
    15061 acgcgtggca atgaaagaaa tcaaagaatt gaatactgaa aaactttcca cgatgcgctt
    15121 tttgcgcgaa agtcgacaca gtgaaatact gattattttt acggaagact tgtgtgctgt
    15181 aatcggtttg gtcttggctt tagcaggaac aatattgact tatgcaaccg ggattgctgc
    15241 atttgatgcg atcagcggat tgctgatcgg tttcttgctg atgggagccg ccattttcct
    15301 agcaaaagaa ttttatagtc tgatcatcgg tgaaagcgtg acagcaagcg atttatcaaa
    15361 aatcaagtta gcttttgaac gtcctgagat cagtcggctg atcgatataa aaacagttca
    15421 tttaagtcca acggagattt tagttgcagc aaaagcagat atcgttggta acaaagagaa
    15481 tgaagcttac tcggtcatca atgatattga aaaaaatatg cgtcagtctc ttcctgacaa
    15541 gaagatgtat atatacattg aaacagataa atatgaccca aattattctc gtaaataaaa
    15601 cgtttattca tgaattgaag caaaaatgag aaaaatataa tcttgttttt aaataaaacg
    15661 gctacaattt agcgaattta tgttacaatg agtccgctgt ttatttgttg taattaaagg
    15721 agatatttta atgaacaaaa agaaaagtgc gccatggctt caagctttgc atgcagtgat
    15781 gccactttgt ataagctata taccagtagg attagcttgc ggagtattgt tgcaaaaagt
    15841 tggatttgat cctctcttat caggactgtt atcgattctg gtcttttccg gtggcgcgca
    15901 gtttttagtt gcatctatgc ttacgacgca agcatcattt gcaacgacat tattgatggt
    15961 tttcttttta gaattacgct atacattatt aggagcaagt cttgccggtt ttatgaaaaa
    16021 agaaaagcgg acgtttttgg ctgtcttttc tcaatcactg aatgatgaaa actatgcggt
    16081 gaactactta aagttttcaa ctgacccttc ctggaataaa agaaaggctt tatatgtcaa
    16141 ttggttctca atgactgcat gggccggtag caatatgatc ggtaattttt tcggctcggt
    16201 gattcgagta gatgctgacc ttgtccattt tgctttgacg gccatgttca ttttcatgtt
    16261 tatcatgcag atgaaaagcg cactactgat ctttacaggt ttattctcag gcgtattagg
    16321 ggtcgtattc atggtattat tccaaaatac tttcggattg attgcagcaa cagtcattgc
    16381 ttcatttgcc ggatttatgc tggagaaggc attcaaaaaa gaaaaagcta agaaactaag
    16441 agaccgcggt aaagatgtca atgagccgct cttcaacttc ccggaacagg aggccaatca
    16501 ccatgactaa actatatttg ctacttttag ttgtttgtct gttcatcgtg gcatatatcc
    16561 cgcgcttgtt cccgatgctt tattttactc atcgaaaagt gccatcctgg ttcagtgact
    16621 ggatgaaata tgtaccggtg gcattatttg cggcgttagc ttttaaagat gttttcatta
    16681 ctcatgaaca tctggatatt gcttggaata tcaaaatagc tgcaatgatt ctagtagcag
    16741 gcgtggctta caaaacacgt tcgatggcgt tgtcagtttt gacaggactt gcgtctgtct
    16801 ttttgctgtc tatgctttaa actgttttat tttattaaaa acagagattg cgacataagt
    16861 tgttcaaact gatctatccg aactgcattt gcaacagtca attctggatg caagtttatt
    16921 cgtagggata aaaaagatta gtgttggctt atgatgcaag ctctttttta ttttaggaat
    16981 tagagaggga tttgtccttc tatgattttt tgctataata gggaagatgt ttattttatg
    17041 aaagaatagt tgagggaatt taaatgaata aaaaattgat gcaaggaaca ttttggctaa
    17101 cctttgctaa tcttctatgt aaagttttag gggtagtcta tttgattccc tggctaagta
    17161 tgatggggaa caatcaagat ggtatgcttg ctacgacatt gtataatgtt ggctatcttc
    17221 cttatggatt gttcctgatg cttggtacag tcggatttcc aaatgcgatt gcaaaaaaag
    17281 tagctgtagc cacaaaagaa ggagataacg ctgcttgtcg gctgattttt agaagtacga
    17341 tcaatatcat gtttgtcatc ggtatcgcct ctgctgcact gatgtattta tttgctcctt
    17401 tactagccgg aatcagtccg attgctaacg ttaataatgg gatactagct atcagaagtt
    17461 tatgcccatc tttgattgct atcccaattc ttagtgcaat gagaggatat ttccaaggaa
    17521 aaaatgatct gcggccttat ggaacttctt tgattatcga acaggctgtg cgagtgatcg
    17581 ttatcttggc tggtacttat tacttgcgcg tattgactga cggaaccata ttagaagctg
    17641 tattgatcag tacagtcgct tctttcttcg gtgggttagc tgcgattatc catatgtata
    17701 tcgtcggacg cagaaaagac ttcttcgaac ttaaagattt tttgatttcc agccgttatt
    17761 tcgaaagaga aaaccgatct gcctctgtct ctatcattcg ggaaacacta ccgtttattt
    17821 ttgtcggctc agtcattact atcgttcaaa tgatcgacca agtaacgatg aaaccactgc
    17881 ttcacttttt ccgtccagag atcgcagatc agcaactgga attcttattc agccgtgctt
    17941 ctgtcaatcc aaataaattg acattgatcc tgatctccat ggtaggaaca gtagctataa
    18001 gcagcttgcc aatattaagc acgatgaaga aaaaagatcg gatacaaatc gaaaaaacag
    18061 taggagatag tttttcgatt gcgttattga ttcttcttcc ttcattgact ggaatgtctt
    18121 tattagctgg accactatac acattgtttt ttggctatga tcctgaaagt gtcggatatt
    18181 tccaaatggc tttgctagct tctttgtttt tctctttatt cacgatccta tctacgatgc
    18241 ttcaatcttt gaatcaccat cgttttgcga tccgattaac tgtagaagca attatcctga
    18301 aagtcatctt ccaagtaatc ggattaggat tagttggcgg atacgggatg agcttatcaa
    18361 atggcttagc attcggtatt gtctttgttc gcggctatca ctttatgtgc aaagaatacc
    18421 gaatagcacc tttagcaaag atcagcaatt ttttcttaaa aactttccgc tccaccctga
    18481 ttatgcttgc agtatgtttt gccgttttct ttttgctgaa tcagcgattg tcaatggaat
    18541 caaaaagcta tgcaatgatt tattgtgtgg ctgtaggaag tattggcggc ttagtatttt
    18601 tattttctca attcggaaaa aatgggtttc gaatgttgaa aaatgttaaa catcatagca
    18661 gaagcaaaga ataggttaac taatcgtttt ttgctgtcac ttgaaaaacg gcaactcgta
    18721 tgattacgct tgtcgttttt ctattttcca attttagttt catatctaat cttacttatg
    18781 atatagttat aaggtctgga aaaattcttt gaaacgagga aattaaacac atgaaacttg
    18841 cagtagttac agatagttcg gcatatctgc cggaacgtat caagaaccat cccgacttat
    18901 tcgttattcc aatccctttg attatggatg ggaaaattta taacgaagga atcgatatcg
    18961 aagcggatga atattatgga cttttaaaaa acagtaaaga ttttcctact acttcacaac
    19021 ctgtcgtagg ggaagttttg ggtttatatg aggaattgaa agagaaaggg tatgacacga
    19081 ttttaagtat ccatctttct tcagggattt ctggctttgt caacacatta tttgcgatga
    19141 aagatgacat cagcggagta acggtcattc cttatgactc taagatcact agtatgccga
    19201 tgggacacat ggtagaagct gccttagact taaatgatga aggtcagtcc cttgatgaaa
    19261 tacttgcaca tatcgatcga atccgagata atacctacgc ttatctgatt gtagatgatt
    19321 tgaacaattt agtacgagga ggacgtttga caaatggtgc agcgctgatt ggcagcttat
    19381 taaaaatcaa accgatcctg acattttctg aagggaaaat tgttttattc gaaaaaatcc
    19441 gttcaagtaa aaaagcattt gctcgagctg aagaaattat tggtagaaga aataacgaaa
    19501 tacaaccgcc agtcaaactt tacgtgatac atgcaaataa tttagaagta gcgattgcgg
    19561 agaaagagaa attgcagaaa aaatatccaa atgcagaaat cgaaattggt catttcggac
    19621 cagtcattgg tacacattta ggtgaaaaag ccatcggcat tgccatttca gcgcaataat
    19681 tccttttatt caaaaaacat accaaaaata ttccaacatc atcttggact ttctacatgt
    19741 tactaatgag cgtgtaaaag aacgagatgg tgtttttatt tctatcaatg aagctctttt
    19801 tctttgcaat cctcacttca atcctctata atgtcgcata tagtttagta aatgagtaaa
    19861 ctactaattt acttatttac taaaaataaa aggatgagga gttaaaataa tggaaatacc
    19921 aacaaattta aaacataaac cagtaatcgt tgtagaagac tatgataaag ttgatggaag
    19981 aaatgcatta aatacggatg caaaagggct ttcactgggc cttgctcagt ggaatgatcg
    20041 tggaaaagtc gatatctcag gcaaggtatg gcgacatact ggagaaaaat ggtcacgaca
    20101 gtcggaagaa ttaccaatca cacgtatttt agatttagcg atcttagctg cacaaggaag
    20161 tttatacttc caagatgctt atcgttatga aaaattttat gatccagaaa atccagtcgt
    20221 agacatcatt ggattacaag ggaaccgaat gacggtagaa gtagatacca aaaatccaaa
    20281 gatcgatgaa gacatcattt tgtattacga tacactacaa aaagacggag aactgattgg
    20341 ggaacgattc cgtattttaa aacgtctttt agatgagcta ggatattaat agacagagat
    20401 gatcaaaagg ggaagagaag catggagaat ttctcactaa cagaaaaccg aaccttttca
    20461 cggctagaaa agcaaatgat ctggaagaaa ccgaaaagcc atcggacaag tgcagaagaa
    20521 tggcggatcg cctccgaaat caaagggaat tggcaagatc ctgaaatgaa aatcagtaac
    20581 gtacttttgg aaggagatgc cgggtcgggg aagacacaat tagccaaagc actatctgct
    20641 gatctgcaac ttccctatac aaaagtgacc tgttttgcag atatggacaa atcagatgtt
    20701 ttcggagcct tattacctgt agtagattct aataaggaac aagaccaaga attactagaa
    20761 gctatttacc aaacagatac acttgaagct gtactgacac tgatccagaa ccattacggc
    20821 attgatcctt tttctgctaa agaaaaatta gcagagctag ttcaaaggat cgaagctgat
    20881 tatccagatg caatccatta cagctattac ccttctgaaa tcgttcgagc gattgaaaaa
    20941 ggatatttat tagagattca agagccgact gtcatccgag atgcctctgt tcttgtggca
    21001 cttaactcag cgctcgaacc aaatgggatg ttgaatttac cgacaggaat cattcgtcgc
    21061 catccagatt gcgtcattgt gatcactaca aatcgaaatt accaaggaaa tcgcccatta
    21121 aatgaatctt taagagatcg gatgcaacat gcggaaaaaa tggatcttcc tgccttagaa
    21181 gtcatggcag aacgtgcgat ggcgaaaact atgatccaaa aacaagaact tctcttaaag
    21241 atggcagaga ttatccgttt gttggatgaa actgcaaaag ctaacgccat caaaggtgta
    21301 gcaggaatgc gttcttattt ttattgggca aatacctata agcaaggaca agatttactc
    21361 cagtcgattt atccaaaagt tttgtataaa ttatccactg attcagatga attggcaatt
    21421 cttgaacaag ctttgtctga cagtggattg ctactggaac ttcaggacat attgagaaaa
    21481 gaaaaatggg gtcatcctca atctgagcat acaaaaggaa gaaccattag cgccgaagaa
    21541 gcaaatgaac gaaacatcac tgaaaatgct gaagaaattc tgcgcttaga tgaaaagaaa
    21601 tcgttgtctg ctgaagaacc aaatgaggga gaagagaaac aagaagaaac aatagaaaaa
    21661 aacgagattc ctcctcaaat gcaaaaagaa cgcagtagcg atatcgaagg aaaaaatcaa
    21721 aaagaacaac aaacaggaga tgattctcaa gaaatgtctg cttctgacat ggaagcacat
    21781 gataaggaaa tcaagaagca actgaataaa gaagcgcgac aaatcatgaa ggaaacgatt
    21841 catgaaaaag aaggtctcat cgttcaccga ccagctttcc aaggaaaaac gccggaagca
    21901 cgtgagctac acatgcaagt aatgccaata gtagaatcac ttagccggca aattttagag
    21961 ttactggata atgaacaaac agagacgtac caaaaaggaa aatacgaagg tcagcggttc
    22021 aatgcaagca aagtagcata tggtgatttg agaaattttg acaaaaagaa cccgccacat
    22081 gagcaacctt ctttagcggt agctgtacgg atcgatgaat ctgggtcaat gatacgggat
    22141 gatcggatcg aagcggcaaa aaaagcagcg attgccattg ccgaattttc taagaaagtc
    22201 gatatcccat tgttgattta cggagataca gcagaccgtt cgtcaagaga aaagacttct
    22261 ttgttttctt ataaagaatt tgaagacgga tttcaatggt taaatgaaaa attagtcact
    22321 atgaaaccaa ggcaaaacaa tcgtgatggt gctgcacttc gtttagcagc agaaaaatta
    22381 aacagacaat ccgcaacaac caaactgttg cttaatatta gtgacggtca gccaaaagcg
    22441 ttgccggatt atacaggcaa aaaagcaaaa gaagatatcc agtctgtttt gaaagaatat
    22501 gaacgtcaag gaatcctatt tgtctccgca gctattggcc aagacaaaga agaaatcaag
    22561 tcgatctacg gtgaaagtcg ttttgtagat attactgact tgaatgaatt tcccaagcaa
    22621 atgattcaac tgattgcccg ctatctatga taaaataaag ctcaacgaac gcacttagca
    22681 gattcgttga gcttttcgtt tctagttacc ggtaacaagg atacttctaa aatagaatcg
    22741 gttataatca agagggaccg ttaaaggagt gaaacgatat gatacaagaa caaaaatggt
    22801 ggaaagaagc agtaggctat caaatctatc ctgctagttt caaagatagc aatggcgatg
    22861 gtcaaggaga catcaatggg atccgtgaaa aacttaatta tttgaaagat cttggtatcg
    22921 gctttatctg gatcaatccg atctatcaat ctccatttgt cgataatggt tatgacgtaa
    22981 gtgactatca agatatagaa gagagattcg gaacgatgga agattttgat ttattgctaa
    23041 aagaagcaca tgaattaggg atcaaagtga ttatggatct tgtcatcaac catagttcgg
    23101 atcagcatca atggtttgaa gaatcgaaaa aatctaaaga aagcccgtat cgcgattatt
    23161 acatttgggt cgacggggta gatggcaaag aaccgaataa ttggacatcg attttcggcg
    23221 gttctgcttg ggagtattct catgaaactg gacagtatta tctgcatgtt tttgcaaaag
    23281 aacaaccaga cttaaattgg gaaagcgaga aattgaaaga agaactattc aatatgatcc
    23341 gatggtggtt ggataaaggg atagatggat ttcgtttaga tgcgatttct catgtaaaaa
    23401 aagatgaata ttcagtaaaa gccacagaga accctttctc accctttcaa aatgtctcag
    23461 gaatcgaaga acatttgacc gatttgaaac atgtttttga agagtacgat atcatgactg
    23521 tcggagaagc aagtggtgtc actgcagaag aaggaccaca atgggtaggg aaagacggtt
    23581 atttcgatat gatattcgaa tttgatcata tccatatctg gcagcaagaa aaagaagggc
    23641 aattagatgt ccttaaattg aaacttgcat taagcgcttg gcaaacttca ttggatggta
    23701 tcggatggaa cgctttatat atggaaaacc atgatgttcc tcgtgccgtt tcagtgtttg
    23761 gagatactcg tcccgatttt tgggctatgt ctgcaaaagc aatcgctatg atgtatttct
    23821 ttttgcaagg tacgccattt atttatcaag gacaggaaat cggaatgacg aatatgccct
    23881 ttgaatcgat cgatcaagtt gatgcagtag attcgaaaag actttataaa aggctgcttg
    23941 cggaaggtaa gacaagagaa gaagcattgg atatcattcg tgaaacaacg agagacaaca
    24001 gcaggacacc catgcaatgg acttcggaac aatatgctgg tttttctaca catgaacctt
    24061 ggctgatcac taatccaaat accaaaacga tcaatgtaga acaacaagaa tatgaaccag
    24121 agtcggtcct gcaattctat aagaacatga tccgcattag acaaaccaat aaaggtctga
    24181 tatacggttc ttataaagaa tatcttcatg aacatccaca actttatgtc tatgaaagat
    24241 atttagaaga tgaacaatat cttattatgg taaacttaac agaatcactt gctgactatg
    24301 aacttccaaa agaagcagat cagtcctgga cattactctt gtcaaattcc tctagcggag
    24361 aatttgaagc taaaggcata ctagcacctt atgaagctcg gttatataaa acaaacaagt
    24421 aaaacttcct cgttaaagtt cattagttgg actttgcgag gaagtttttc agattgctct
    24481 gtcaattact catttcaata attcgctcta ggaccaccga tatatttagg acgagagcga
    24541 agggctgtca cgtgagcttg tccaagggtt ttcaaacgag gtctgacagg tacttggaca
    24601 tgtttgatat gcataccgat tgatgtgtca ccgatatcga taccggcttg tccaactacg
    24661 tgttccactt cgacaggatc attgaactgg tcaaaagcag caacagaaca agcacctcca
    24721 gcatgtagtg caggaacaac agagacaatt tcgaaatttt ttgcttctgc cactgttcgt
    24781 tctactacta atgctcggtt caaatgttca caaccttgga cagctagcgc aatttcttta
    24841 ggatccaaca actcttttaa cgtacggatg atccattgac caacttccgc actggaattt
    24901 tttccaatat gtcctccaac gacttcgctt gtgctgcatc ctaaaacaaa aagatcacct
    24961 tttttcaagt gtgcctcagc caatatgtca ttaactattt cagtcaattg gtcttttaat
    25021 tctttttctg acaacttcat tttctaaaac cttctttttc aattaatatt gcttccgtgc
    25081 ttgaatacgt tgaagacttg cagttatagg aaaggcaagt cccattccaa tcagattttg
    25141 gaagatgttt cctggtatag aagccactcc agccggccaa tcatataaaa tccaaccggc
    25201 gataccatag ccgatgatca taactaagct tcctaataaa agccctaatc cccacatacg
    25261 agtagaagat ttgctgaaag aaccagcaac atatccttgt aacccgtgga tcactaatga
    25321 aaaaactgcc cactgcggat atcctgaaat caaatcgatc aatagaccac tggaagcacc
    25381 aacaactaat ccacccgttg gaccataaag aaaagcagcg gtatagatcc cggcctcgca
    25441 taatgtcaca aatccattcg tagccggtac agggatcaaa attgttaatg aaagggcaac
    25501 tgtcaacgca atcagcatag catagatcgt taattgctta gttttcattt ctcattcctc
    25561 ctttttgaat accgccagac cagacacttc cggtatgatc gttcatcagc acgccattag
    25621 aaatacaatg ggcaacaaat tttttacttt ctgctactgc tttggtaaaa tctgccccta
    25681 acacaagatt cgctgttatt gcagaagaaa aggcacaacc ggccccgttt actgttgtag
    25741 tgtcgatttt tttatgagaa aatacctcat aatgatcaat tgaatagaga agatccacag
    25801 cttcatctcc agccatcctt gtgccgcctt tgatcacaat gttttttgtt cctgaagcag
    25861 tgagttcttt agcgagatgg atcatttctg ttaacgaaga gggaagagct tgaccagaaa
    25921 gtaaagcagc ttctgccaaa ttaggcgtaa tcatcgtaac aagtggaaaa agttcttcga
    25981 tgagctgcga aatatacaat tgattgatca cagcttcagt ttctttgaat gccaaaacag
    26041 ggtctaaaat gattgggata ttttgttttg tttttatgaa gtctcgaaca accatgattg
    26101 cttcacttga attcaataac ccaattttga taccatctaa tgaagtcatt ttatcaatcg
    26161 aaactaactg tcgtttaagc aattccaaag atatagattc cagcataaat cgctcattaa
    26221 tatctagtgc ccctatagaa gtcaaggcgc ttagtccgaa tagaccaaaa ttctcaaaag
    26281 tttttaaatc tgtttggatt cctccaccag caaatggatc agaaccgcca attgtcaaga
    26341 ttcttttcat aacattttcc tccatgaata agagtataac gaaattttca aaaaagaaaa
    26401 acagccaatt ggcatatttt tatctcatcc aatttaaaaa aacaaaaaga aaagaaagac
    26461 caaaatggat ctttctttcc ttaaatagtt aggacttttg tgattttaat tctttctcga
    26521 tttgaagccg ttgcactttc attgtagctg ttcgcgggat tgcatcaaaa tcacggatga
    26581 tagggacgtt caagcgagga agttcatgga cttgctccca ccatcgattc caatccattt
    26641 ctttatcagg agctaaagca ataatcggtt gtggagaatg atttttatcc cttacaatca
    26701 ctacttctgc caaaaaatct aacgaatcta ataaaaagtc ttcaatagct aatgtactat
    26761 tgatcgtttc gatcaaatcc acttgccgat ctttcaaaaa taggtggcca tgcttatcca
    26821 taaaaccgta atctccgcta tcccaccaat cgccataaac attttcctgg aaccgtgcat
    26881 cttccttgta ataagtcaaa gcccgtcctt tagacagtaa ttgtatatga ccatctgtgt
    26941 tttcaggtag gacattccca actgaatctg taatccgagc gcttgtcatg tcttccagtc
    27001 caacgcccat atctcttgca tcacttgttt tcaaactctc tagtgtatga gccttaagaa
    27061 tcattggtcc gcattcactt tggccatata cttgaagaaa taccgcatcc tgtgccatcg
    27121 acgttcgcaa aaatgtcttc attgtctggt tgtttatagc atcaaaagtg gagtgatagt
    27181 atcggatacc cgaaaaagct tctggatgct ccttagcagt aaaacgccac tgaacaaagt
    27241 tattcggatg ggtctctagt gcaattggcc ggtgagcttc caacatatga acaacttttg
    27301 aactttgtgc gtttgctaaa ggcatcatag gaaatcccat ggacatcaaa gaagagatac
    27361 caatattgaa tctggaatgc actggagaga tgtgaaaagc gattaatttc ttttccgaaa
    27421 tttttgtgaa aactgttttt tgccatttcg tccgccatcc catagaatga gcagaatgac
    27481 aaataagttt agggatacca gttgtaccag aagtatgggt catataagaa atttcatttt
    27541 tgtctaaaaa tgattgagaa acagacaaag caggttgttc cattaaataa agaatagga
//
LOCUS       DAFEOM010000029        27415 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.030, whole genome shotgun sequence.
ACCESSION   DAFEOM010000029
VERSION     DAFEOM010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.030"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(106..1293)
                     /locus_tag="LP524_001459"
     CDS             complement(106..1293)
                     /locus_tag="LP524_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBL2234520.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLEKISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
     gene            1615..2457
                     /locus_tag="LP524_001460"
     CDS             1615..2457
                     /locus_tag="LP524_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBL2234521.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            2457..3404
                     /locus_tag="LP524_001461"
     CDS             2457..3404
                     /locus_tag="LP524_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBL2234522.1"
                     /translation="MRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVVT
                     NSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETGG
                     NAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKNI
                     EKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYFS
                     KAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDTN
                     IPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            3419..3652
                     /locus_tag="LP524_001462"
     CDS             3419..3652
                     /locus_tag="LP524_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HBL2234523.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            3983..4567
                     /locus_tag="LP524_001463"
     CDS             3983..4567
                     /locus_tag="LP524_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL2234524.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            4698..5365
                     /locus_tag="LP524_001464"
                     /pseudo
     CDS             4698..5365
                     /locus_tag="LP524_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
     gene            5376..6791
                     /locus_tag="LP524_001465"
     CDS             5376..6791
                     /locus_tag="LP524_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL2234525.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQNPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            6809..7378
                     /locus_tag="LP524_001466"
     CDS             6809..7378
                     /locus_tag="LP524_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HBL2234526.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            7488..9242
                     /locus_tag="LP524_001467"
     CDS             7488..9242
                     /locus_tag="LP524_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2234527.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            9202..10968
                     /locus_tag="LP524_001468"
     CDS             9202..10968
                     /locus_tag="LP524_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2234528.1"
                     /translation="MQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            11491..11760
                     /gene="rpsN"
                     /locus_tag="LP524_001469"
     CDS             11491..11760
                     /gene="rpsN"
                     /locus_tag="LP524_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBL2234529.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            11775..11954
                     /gene="rpmF"
                     /locus_tag="LP524_001470"
     CDS             11775..11954
                     /gene="rpmF"
                     /locus_tag="LP524_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBL2234530.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            11986..12363
                     /locus_tag="LP524_001471"
     CDS             11986..12363
                     /locus_tag="LP524_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234531.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            12384..12533
                     /gene="rpmG"
                     /locus_tag="LP524_001472"
     CDS             12384..12533
                     /gene="rpmG"
                     /locus_tag="LP524_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBL2234532.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            12557..13540
                     /locus_tag="LP524_001473"
     CDS             12557..13540
                     /locus_tag="LP524_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBL2234533.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            13570..13692
                     /locus_tag="LP524_001474"
     CDS             13570..13692
                     /locus_tag="LP524_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBL2234534.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            13710..15233
                     /locus_tag="LP524_001475"
     CDS             13710..15233
                     /locus_tag="LP524_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBL2234535.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFRDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            15257..15496
                     /locus_tag="LP524_001476"
     CDS             15257..15496
                     /locus_tag="LP524_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2234536.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(15689..16150)
                     /locus_tag="LP524_001477"
     CDS             complement(15689..16150)
                     /locus_tag="LP524_001477"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL2234537.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(16345..16563)
                     /locus_tag="LP524_001478"
     CDS             complement(16345..16563)
                     /locus_tag="LP524_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2234538.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            16681..18297
                     /locus_tag="LP524_001479"
     CDS             16681..18297
                     /locus_tag="LP524_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBL2234539.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(18538..19878)
                     /locus_tag="LP524_001480"
                     /pseudo
     CDS             complement(18538..19878)
                     /locus_tag="LP524_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(20154..20309)
                     /locus_tag="LP524_001481"
     CDS             complement(20154..20309)
                     /locus_tag="LP524_001481"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBL2234540.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(20605..20937)
                     /locus_tag="LP524_001482"
     CDS             complement(20605..20937)
                     /locus_tag="LP524_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL2234541.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(21451..21642)
                     /locus_tag="LP524_001483"
     CDS             complement(21451..21642)
                     /locus_tag="LP524_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2234542.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(21736..22122)
                     /locus_tag="LP524_001484"
     CDS             complement(21736..22122)
                     /locus_tag="LP524_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL2234543.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            22816..24021
                     /locus_tag="LP524_001485"
     CDS             22816..24021
                     /locus_tag="LP524_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HBL2234544.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            24038..24472
                     /locus_tag="LP524_001486"
     CDS             24038..24472
                     /locus_tag="LP524_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234545.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            25089..>27415
                     /gene="esp"
                     /locus_tag="LP524_001487"
     CDS             25089..>27415
                     /gene="esp"
                     /locus_tag="LP524_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HBL2234546.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         9407 a         4020 c         5131 g         8857 t
ORIGIN      
        1 caagtaaaaa ttagccaaat caaaaaatat tttataacag ataattccga aaaaagggta
       61 caacaaataa gcttcttacc attagttttt ttgaatgttc aatagctaat cagaaacaat
      121 tatataaggc tcgtttttag tcaaaatagc atgaatcacg taacaaagct tacgagaaac
      181 cgcacctaag gcagttagat gatgttttcc ttcggaaact tttttctggt aaaacgcact
      241 aaaaacagga tcacattgag aagctactaa agccgccgaa aacaaggctt ttcgcaaata
      301 tggagaccct cgtttactca taacattatg cgttgcctca tattgccctg attgtgtcac
      361 ggaagcgtct atcccagcgt aagcaacgag ttttttcggg ttagaaaatt tttgaatatc
      421 tccgatctca ccaagaatag tcgcaccgtt tataggacca attccaggaa ttgtgagaat
      481 aacagaattt aaagtgtcca ttatgtgctt gatttctctt tccgtttcct ttatttggct
      541 ttcaatgaat tttaattgtt caatcattga acgtagttgg aaggtgaacg cttcttgagc
      601 aaattttact ccaaaggagt tagaagctgc ttgcttcaat tggtccgctt tttctgcctt
      661 gattcggttc ctgcttaact cagctaagta ttgggccaaa gtttctgagg aaattttttc
      721 taagtcgatc ggggcactaa acgccagcaa taactcttta gaagcttttc cgaaaacgcc
      781 ctgcttggta aaaatcgtcg cgtattctgg aaagacttga tccaatacgg ctataatttt
      841 tcttttgaag tcgctcgctg tacctactaa ataagttcgg taacgagaaa gttgtttcaa
      901 agaaaaaaca ttttcatcgg ataagaccgt ttcgacaaat gagccatagc gcattaagtc
      961 agcaatcaaa acagaatcaa taatatcgtt ttttcgtttc cgtatttctg tacctttacg
     1021 ccatccgtct gtttgaaggg gattcacaac gtgaatcaaa taatctgatt cgtgaaggta
     1081 tgaaaagatg gacagccaat agtgccccgt ggcttccatc cctataacaa agtcactggg
     1141 tgctccggaa tagtcgacca ttcgctcaat taatgactct gctccttcag tggtgttagg
     1201 aaatgaaaaa ccttttagta atacctttcc ttcctcgttc atgatagaag caacgtgagt
     1261 gcgtttaccg atatcaatgc ctaaaaagta catgtatatc tgacctttca aaaaaagaat
     1321 tttggattga aatccactca actgaaaagc gtcactacct tgtacgagat acggagtaaa
     1381 gtaaggactt tcaacatcta actcatacgc aatccactta tcagagagag gagccagtct
     1441 ctcgagtgcg ggttcaatgg cccaaggggg gaacggctta gcctctcaat ccaataaata
     1501 tattatctca aaagaaatga gttaatacca aattaaaata acgatggttc atagggttat
     1561 ccaatcaaaa ccggaaggtt aaaaccctaa ctctaatata caagggggaa taatatgctt
     1621 caaaatttta ttaatggtct gtatgacttt cactttttac agaatgctct aattacatct
     1681 gtagttatag ggattgtttc tggaacagta ggttgtttta ttattttaag aggaatgtct
     1741 cttatgggtg atgctatttc tcatgctgtt ttgccaggag tagcaatttc ttatatttta
     1801 ggtattaatt tttttattgg tgcgattgta tttggtctat tagcttctat cattattact
     1861 tttataaaaa gcaatagtgt aataaaagga gatacagcaa ttggtattac tttcagttct
     1921 tttttagcat taggagttat ccttatagga gttgcaaata gctcaacgga tttatttcat
     1981 atcttatttg gaaatatttt agctgtacaa gatattgata agtggattac aattggcgtt
     2041 tctgttttag tgttggttac agttgttcta ttttttaaag aattattaat cacttctttt
     2101 gatccattaa tggcaaaagc aatggggatg aatgtcagtt tttatcatta tctattaatg
     2161 attctattaa ctttagtagc agtaacagct atgcaaagtg tgggaactat attaattgtt
     2221 gcaatgttaa ttacaccagc tgctacagca tatttatatg caaacagctt aaaaactatg
     2281 atatttattt cggcgacagt cggagcgtgc tcatcattgc taggtttgtt cattggttat
     2341 agttttaaca tagcagcagg atcaagtatt gttttaacag cggcattctt atttgtaatt
     2401 agttttctaa tctcaccaaa acaaaaattt ttaagaaaga aggagagaaa gttatgatga
     2461 gaaaatggaa agcagtactg ggaagtctgg ggattttgat tgctcttttt atattcgggg
     2521 catgttcaac aaatagtaaa gacaaagata cagtggcttc gaacgaaaaa ttaaaggtag
     2581 tagttactaa ttcgatttta gcagatatta ctgaaaatat agcaaaagat aaaattgatt
     2641 tacacagtat cgtacctatt gggaaagatc cccacgaata tgaacctttg cctgaagatg
     2701 ttcaaaaaac ttcaaaagca gatttgattt tttataacgg tgttaacttg gaaactggag
     2761 gaaatgcttg gtttacaaaa ttagtaaaaa atgcgaacaa agaggaaaac aaagactatt
     2821 ttgcagcaag tgatggcata gatgttattt acttagaggg tcagagtgag aaagggaagg
     2881 aagatcccca tgcttggtta aatttagaaa acggtattat ttacgctaaa aatattgaaa
     2941 aacagttagc ggaaaaagat cctgataata aaaaattcta taaagaaaat ctagataagt
     3001 atattgaaaa attggattct ctagacaaag aagctaaatc taaatttgct tcaattccga
     3061 atgataaaaa aatgattgtt acaagtgaag gatgctttaa atatttctcg aaagcgtata
     3121 atgtgccttc tgcttacatt tgggaaatca acactgaaga agaaggaaca ccagatcaaa
     3181 taaaacactt agttgaaaaa ttacgcacaa caaaagttcc ctccttattc gtagaaagca
     3241 gtgtggacga tagaccaatg aaaacagtat caaaagatac caatattcct atctattcaa
     3301 cgatttttac tgattcaatt gcagaaaaag gacaagatgg tgatagttac tatgcgatga
     3361 tgaaatggaa cctggataaa attgctgaag gcctttcgaa ataatttgga gggtaggtat
     3421 ggtacaaatt aaaacgacta ctatactaat ttatttacta gcaattcagg aattatcaaa
     3481 aagaaaaaag aaattaaaaa ataatgattt agcaaaagtt ctaagtgtta gtccagcatc
     3541 tgtaagtgag atgctggcta aacttagata tgaggactat ttagatatag gatttcaatt
     3601 aactgataaa gggaaaaaat ttattgacga ttacaaaaaa ggttttaagt aaaagacttt
     3661 atttttaggg aggataagaa aatggctatt ggagtgatta tgataggact taccctaata
     3721 gctggatata atttttttaa atctttctcg gattatttta gaaaaggtga atgatttaga
     3781 tgagtctatt aattcttgta ggattgcttt ttgtatcagt tattttgctc tttctcatta
     3841 gtttagctct aaggaatgag ttcagtaata gacgttaacg attgaaactt caagtcctgt
     3901 gtaagataat atagttaatt catcttatta ggaggaagta cccatagaaa ccagagatta
     3961 tattacaact caccaaaaat taatagaaag tggactaagc cattttgaaa aagaaggatt
     4021 tcagcaaact aagataaggc aactgtgctt gaccgcaggc gtaactacgg gtgcttttta
     4081 taaacatttt acttctaagg aagcgttatt tgaagaaatt attcagccgc atattaatgc
     4141 gcttattaat atgtataatg aagagtttac caaatacctc ttaaacttag aaaatataga
     4201 tatagagaaa agttggagag agaacttcaa tgaattagat ccatttatta attatatttt
     4261 tgagaataaa cgtatattcg atttggtttt attccaatca gctggaacca aatatcaaga
     4321 tattgttgaa aaaattacat catttgtgac aaaaaagaca gtctgttgtc tagataagtt
     4381 aaagactatc ggaatgatca atcaaaattt agtctttaat gaagaagaaa tccacttcta
     4441 catttactca ttctacgcta cattctatga tatattgaag catagctatt caaaagaaaa
     4501 gactatgcta ttaaccagaa gattgtatca attttctgtt cctgggtgga ttgaattact
     4561 gagttaatta aaaaaaagct tgcattgaga attgttctca gttatgcttt ttgataagat
     4621 cgcactgtta tctgaataac ggtgcatttt tgtcagctta agataacagt gcgatcttat
     4681 tttttggagg tgaaagagtg tcaaaaatca atccgttaac cttgattact ttaaatattt
     4741 ttttccggtc attatttttt taggaaaggg aaatatattt caagtggggt gtttcattat
     4801 tgctacagtt ttagtattga tgtttcgtaa aataaagaca tgtatttatt tcatcttgat
     4861 ttattcagtt ctgtttttag taatgaaaat tattgatgct atcaagatag agttttttac
     4921 tatatttttt gggacatcct tatatattgt atttcgaatg ttgccggtcg taatgatctc
     4981 atggttgttg gttagtgtct atactagcaa tgaattatta tcctctttag aaaaaataca
     5041 tttaccgaaa aaaataatgt taagtattac agtagtgatc agattctttc cgacttatcg
     5101 ttctgaaata agaatgatta aagaatcttt aaaaatgaga aacattcgct tgtctttttt
     5161 tcagcctctg caatatttgg aatttctgtt agttcccgtt cttatacgtg caactatgat
     5221 tgctgaagaa atgaccgcta atgcaataac aaaggggata gaatctcctg tgaaaagaag
     5281 ttcttactat aaaacaaaca tgagcttatt agactatatc atcctgtttt ccgttactat
     5341 ttttttctta agtattttga tttgaggtga aagaattgtt tgaatttaac aatgtttctt
     5401 tttcatataa ggatgttaag gaacaagcgc ttgttgacct taatttttca ataaaaaaag
     5461 gcagtcttac attgattact ggttcttctg ggagtgggaa atccacacta ttaaagttaa
     5521 tgaatggact tattccaaga ctttatgacg gaaaattggt tggggaaatt ttatttgaaa
     5581 ataactcgct acaatcctta acacaagaaa gtatatcaaa aaatattggc tacgtaagtc
     5641 aaaatcctag aggacaattc tttacaacaa atacaacgag tgagttagta ttttcgatgg
     5701 aaaactttgg atttacaaaa gagcatatgg ataaaagaat caatgaatta gctgaattat
     5761 tagatctttc aaatatatta gataaaaaca ttttttcgct atcaagtggt gaacggcaga
     5821 aaatttctat tggatgtagt ttatcgctaa actctaaatt aattatattg gatgaaccgt
     5881 cttcaaattt ggacttcaaa gcgactaaga aacttgctga cttactttgt gatttaaaaa
     5941 ataaaggatt tactattgtt atagctgaac atcgttttta ctatttgaaa gaattaattg
     6001 atcaagttct atttgttaaa aatagaactg taaaaactac aactattcag ttgttaaaaa
     6061 ctgaaaacaa taacgcatta aggaaatttg acatttttaa tagttcgatt gaagttccct
     6121 atatcgaaaa ggtaagtgca gatgtattaa aagttgaaga aattacttat gaaaatattc
     6181 taaaagacat aaattttgat gtcaaagttg gagatgttat tggtttggta gggaaaaacg
     6241 gtgtggggaa aacaacctta ctaagaatac tcatgaagat tattcgccct accaaaggaa
     6301 agataatcga aaataaaaaa aatttgtcat cgccgttttt agtaatgcaa gaaatggatt
     6361 accaattttt cacagaatca gtgaggagcg agttagccct ggggaatgaa atagttagta
     6421 aagatcagca agaaaaaatt ttaaaaaaaa tggaacttta taaaatgaaa gatcaattac
     6481 cgtttgattt atccggtgga gaaaaacaaa gattattggt ttctatagct tcattgtcaa
     6541 aaactaattt atttctattt gatgaaccta caagtgggtt ggattatatc aatatggaaa
     6601 ggattgctga ccttattaaa gacttaaaag aaaagggtgc agtagttata gccactcatg
     6661 atccggaatt tctttataaa acatgcaata gaattattta tttaaaaaat ggtggaataa
     6721 aaaaagatat aaaacttaga gtagaagatt caactttgat agaacagatt tttaatgaca
     6781 taacgaaata gaattgagga gaatatatat gaaaacaaaa gattatattt ttattggttt
     6841 agctacagtt atttccctag taatttactt tttttcaata atggttagta gtattggtgg
     6901 ggcgtttggt cacagcatat ccccagggat ttttggatta ttatctggaa tcatatttgt
     6961 ttatattagc tataactacc ctagaaaagg aatttttact atttacacaa ttgttttatt
     7021 gttatttttt acgttaatgg gcggggcgta cttaccttgg tttatcagtt cgattagtat
     7081 ggcgatctta gctgatatta ttctatctgt atttggatat gatcgagcaa ttcctcaagt
     7141 tgtgtcttgg gttctaatgc aactaggcag tgctgccgga cagtggattc ccatatggtt
     7201 ctttacagat cgttttcgtc aagactggat agatagagga caatctgctg caacaatgga
     7261 tgcaatgatc cactacgcgg tgggtatatg gggaattatt tcagtgttag ttgtagcaag
     7321 tttatctatg attggtgtat tgattggaag aaaagtctta aaaaaataca aaaaatagaa
     7381 cgtagtttac cttctatctg tttgtacatc tctaatttac gctattacgg aaagcattaa
     7441 cagttgtaaa cgaataagag aagaaattga atgcggggga ggaaaaaatg aaagtataca
     7501 aaaaattatt tagttatgtc cctaaaatga aatactacgg atggttagcc actttatttt
     7561 ctgatttttc tgtcgtatta acagtctatg gatattacag tatttacaaa tttcttcagt
     7621 ctctaattat tgtaggagat gaatatttag ctaagagtta tgcggttcaa actgttgctt
     7681 ggttaacttt aggagcttta ttttatattt tttctggctt attttctcat atccttgggt
     7741 ttcgcttaga gactaatttg agaaaaaagg gaattagcgg tttaacagaa gcaagttttc
     7801 gtttttttga tttaaattct tcgggatata ttcgaaagac aattgatgac aacgcggcta
     7861 aaacacatat ggcaattgct cacttgattc cagatagtgc tcaagccata gtaacacctg
     7921 ttttatcagt tgccttaggc tttttcatta actttaaaat tggattggtt ctaatcgcca
     7981 tgttagtaat tggtgtattc ttgttaaaga aaatgacaga gaatacaaat tttattcaaa
     8041 aatatcaaga atcgttagat gtgttaagct ctgaaacggt agaatatatc cgcggaattc
     8101 aagttataaa gatatttggt gctaaagtaa catcatttaa agccttgaat aaagcaatta
     8161 acgattatgc taagtttgca tataagtatt ctttgagtgg gaaaacacct tttgttcttt
     8221 ttcaatggct attttttggc atagtttgca ttttgatagt tcctataacc ttttttataa
     8281 cttctcttac cactcccaag atcttagctg ttgacttgtt gatgttgttt tttttaagtg
     8341 gaattatttt tgtttctttc atgagaataa tgtatgtaag tatgcacttg tttgaagcaa
     8401 gatatgcagt cgaaacacta gactcccttt atttagaaat gaagcaagac caactaaaat
     8461 atgggaacga gacacagatt aaaaattgtg acattgcatt tgaaaatgtc agcttttcat
     8521 acaaagaaga gtgtgtttta gaaaatttca acttagtttt agaacaaaaa agaatttatg
     8581 cattagtagg aaaatcagga agcggaaaga gtacgatagc taaactaatt tcaggtttct
     8641 acaatgtgga taagggacag attaaaattg gtggtaagcc tttaaatcac tattcaaaag
     8701 acacaattat taacacaatt gcttttgtct ttcaagatac gaaactattc aatcagagta
     8761 tttacgataa cgtttctata gcaaatcctc aagcgccagc aagtaaagta tttgacgcgc
     8821 taagattagc cggttgcgaa tcgattttag aaaaatttcc cgaaagagag cataccttaa
     8881 ttggtagtaa aggagtttat ctgtcaggag gagaaaaaca acgtatagct attgccagag
     8941 caattttaaa agatgcaaag attgttatta tggatgaagc cagtgcatct attgatccag
     9001 ataatgagta tgaactacaa aaggcttttc aatatttgat gaaagaaaaa acagttatta
     9061 tgatagctca tcgattatcc agcattaaat ctgtagatga aattcttgtc ttgtcagaag
     9121 gaaaaatagt agaacgtgga aatcataatg aattaatggc taaacatcga gtgtataaac
     9181 aattggtcac taattatgag aatgcaaatg attggaggct tgacaatgaa aaatttttat
     9241 aaaaaaaaat ttgctttaac tgatcagggc gcagaagctt taactaaagc ttctatttct
     9301 agtttttttg tatactgcat taatatggta ccagcattca ttataatgat gctgatagac
     9361 gaattagttt tagaaaatgc aaagccccgt tggctttatt ttgcagtatc gtttgttact
     9421 ttgcttttca tgtattggtt gttagatagg gagtatgaaa acttatataa tagtacttat
     9481 aaagaaagtg cgcatttaag agtgcaaatt gcagacgatt tgtcaaattt accattatcc
     9541 tatttttcaa aacataattt atcagattta tctcaaacta tcatgtctga cgttgaaggt
     9601 attgagcatg cgatgagtca tgcaatacct aaagccggcg gtatggctct gtttttccct
     9661 tttatttcag tgatgctttt ggttggtaat gtcaaaatgg gattagctgt tattttgcca
     9721 acgttattta gttttgtctt aatcttgtta tcaaagaaat cccaaacgaa agccaatact
     9781 aaatattacg atactttgag agaaaactcg gaagaatttc aagaaactat tgaattgcag
     9841 caagagatta atagctttaa tctatctaaa aaagttcaag acagactttt caaaaaaatg
     9901 gaagagagtg aaaggattca tttaaaggta gaattaagta ctttttcagt catggcctta
     9961 tcctctattt tctcatatgt tagtttagca gtagtcattc tagtaggtgt tcacttactg
    10021 ttaacgggtg aagtgactat actctacgtc gttggttact tactagccgc aataaaaata
    10081 aaggattcct tcgattctat gaaagaagca gtgcttgaaa tattttattt agctcctaaa
    10141 atacagcgaa ttagagctat gaaggaaacc agtatccaag agggatcaga tagtcctctg
    10201 aaatcttttg acattgagtt gcgagatgtc tctttctcat atgataacaa taccccaatt
    10261 ttagatcata tctcttttac ggctaaacaa ggagaagtga ctgcgttagt aggtgctagt
    10321 ggttccggga aaactagtat cttgaaatta gtgtctagac tgtatgatta tgatgaaggc
    10381 tgtattttaa ttgatgggta tgatattaaa cgtgtatctc cagcttcttt gtttagcaaa
    10441 atcgctattg tttttcaaga ggtaactttg tttaatacaa gtattttgga aaatatacgt
    10501 attggaaata gtcaagcgtc agatgaggaa gtgaaaaaag cagctcgttt agctaactgc
    10561 gaagacttta ttgaaaaact acctgacggt tatcatacat tggtcggaga aaacggaagt
    10621 tcactttctg gaggagagcg acaacgccta tccatagcac gggctttttt gaaaaatgcg
    10681 ccaattttaa ttttagatga aattactgcc agtttagatg cggaaaatga aaaacgtatt
    10741 caggaaagtt taaatcgttt aatacaagat aagacagtac taattatatc gcatcgcttg
    10801 aagtctattg aaaaggtaaa taaaattgta gtgatggatc aaggaaaact agttgatcaa
    10861 ggtactcata atgagttgta tcgaaggtca gaaatataca aaaatctcat aaagaaaact
    10921 aaactatctg agaaattcgt ctatgaaaaa gaagctcaat cacgatgatg aaataggctt
    10981 taaattaacc tacaattttt gggatatagt tataattctt tttcaaaaag aataatttta
    11041 ttaaacagca tctaattttg aatacacgaa agaatcagta tgattgaatt agttcactta
    11101 atgtctattc ttataatata aggaataatc aaagttttaa aaaattggcg ataaaccagt
    11161 taaacataaa agattgaata aaaagttata gtttctttca tattgccgtt ttaagtagag
    11221 gaaaagctac ttaaaacggt atttgtgttt gattggacta ataaaagtga attcttattt
    11281 ttcagagcct aaactgcata ttcttttaaa tgatttgatt atctctatgt accgataaat
    11341 tcgttttcaa atagttatct attcatatga gttttatttc atgttattta ttgttgacaa
    11401 gagcaactct tgttggtaaa gtatcagtgt caaaacgtaa cgattacgat ttgaggtgtc
    11461 taaatcaaaa taaaaggagt ataaaggtat atggcaaaaa agtcgaaaat tgctaaagca
    11521 aagaaacaaa tggcgatgat tgaaaagtat gcagataaac gtcaggaatt aaaagccgca
    11581 ggagatcgca ccgctctcgc taaattgcca agagattcaa atccgaatcg tctgaggctt
    11641 cgtgatcaga cggatggtcg tccaagaggg tatatgcgaa agttcggtat gtcccgtatt
    11701 aagtttagag aacttgccca tcaagggttg attcctggag taaaaaaagc aagttggtaa
    11761 aaggaggaaa tatcatggca gtaccagcaa gaaaaacatc taaggcgaag aaacggttaa
    11821 gaagaactca tcaaactgta gtaaaaccag aaatttcatt tgacgaagcg aatggtgatt
    11881 atagacgtag ccatcatgta tctttaaaag ggtactataa tggaaagaaa gtaattaaag
    11941 gagcttcaaa atagtttaat atataacttt taggagggaa tcaaaatgga aattatttac
    12001 ccacctttag ttgaagaagg attaaagtat tatcttgaaa cgacccagca atcgctagat
    12061 aaatgcactt tttatcgttc gatggtagag agaggaatta ttactgaaac gggtttaccg
    12121 acccaacagg caattgaaaa tggattagtc aaagattact atgaggacca aggcttatca
    12181 tttgatgatt ttttaagaat ctatccaatt tttgaagagt atgacgagga gttatttcaa
    12241 tgtatcgatg gttattggga gatacctgtt gatatgaaag aaaatttagt ttcacaattg
    12301 gaatctggcg agttaacttt tgaagatgca caacaaattc aagcatatct tgaagatcga
    12361 taagtattga aggaggaaga tttatgcgtc aaaatattat tttggaatgt gtcgaaactg
    12421 gagaacgtct gtatctcacc agcaaaaata aacggaataa ccctgaacgg ttagaattaa
    12481 aaaagtattc gccaaaattg cgcagacggg caatttttaa agaagtgaaa tagctaagtg
    12541 gtaaaaaggt gacaaaatgg gcataccagt tacaatcgtt tctgggtttt taggggctgg
    12601 aaaaacaacg ctaatcaatc aagccttgca agtctcaccc taccccaggg aagaaattat
    12661 tataattgaa aatgagtttg gtgaagttgg gattgatcac aaattattag ttcatagcga
    12721 ggaacgggtg ttacaactaa ataatggatg tatgtgttgc agtcttcgca gtgatttatt
    12781 agcaatattg atttctttac tggaaacaat agaagaacat caacaaccaa tttcacaaat
    12841 catcattgaa actacaggta tagcagatcc ccaaccaatc attcaaacac ttttaacagc
    12901 gcctcaacta agaggaagag tatatatcga tagtttattg actgttattg atagtgacag
    12961 ttttgaactt attgaaagag aaccacaagc ccttaagcaa cttgtcatgg cagatcgcat
    13021 ttttgtgtct agaaaacaaa acttgaattt atctgcacta aaaaaattac agagagaaat
    13081 caaaagtatt aaccctttat ccgatattcg aatgttcaca catagtgaaa cgattcaaaa
    13141 aaaggatttt tataatttga aaaaatttaa tcatccctta gtttttgaaa gtgattcatc
    13201 agaaaatcac tataatggca aatactatca tcaacaccat caagtacatg agtttaaatc
    13261 aattctctta acttcagatc aagacataaa aaaagatttt ttactccaat ggattgactg
    13321 gctgatttta accaaccaag ggagcctgta tcgttttaaa ggattgattg ctttacaaga
    13381 gcaggatttt atagttgcca tgcaagaggt aaacgaacag gttaattttc aatacaccac
    13441 tcgacaaaaa gaccaaacca ctattattct aatcggaaaa atgttggatg aagaaaggat
    13501 caaacagagt tttgataaat tactggcgga aagcaaataa tgataagtag tgattggggg
    13561 taatttctaa tggagaaaac ggatatatct agtgcatatc gacgacttaa aagcccgaat
    13621 attaaaacta gaaaaagggc attaaaaatt atcaaagaac ataaacgaaa taaaatgaag
    13681 aaattagcat aattaaggaa ggaaaaagaa tgaagaaaat cagtgtagga cttattggga
    13741 tagcagctct aggattattg ggagcttgct ccagcactaa tgatgcaaaa gtatcgaatg
    13801 ataaagatgg aaaattagaa attgtaacaa cattctatcc aatgtatgat ttcaccaaaa
    13861 acatcgtcgg cgatgaagcg aacgtggatt taatggttcc agcgggatca gaacctcatg
    13921 actatgagcc ctcagctaag gatatggcaa aagctcatga tgctgatgtt tttgtatatc
    13981 ataacgaaaa catggaatca tgggtaccaa aagctaaaga aagttggaag aaagctgggc
    14041 ctaatgtagt agaaggaaca aaagacatga ttttattgcc aggtagtgaa gaagaagatc
    14101 atgatcacgg agaagaagat caccatcatg aactagatcc tcatacgtgg gtttcaccca
    14161 aaatggcaat taaggaagtt tcaaatatca aggatcagtt agttaaattg tatccgaaaa
    14221 aagcaaaagt atttgagaca aatgctgaaa agtatttgac taaactgaaa cggttggatg
    14281 ctgactatac cactagtctt aaagaagcga aacaaaaaag ttttgtgacg caacacgcag
    14341 cctttggata tttagctttg gattatggat tgaaacaagt tcctattgct ggactaagtc
    14401 ctgaagaaga gccatcttct ggtcgattag ctgaattaaa agagtatgtt aaaaaaaata
    14461 agatcaatta tatttacttt gaaaagaatg ctaatgataa aattgcaaaa actttggcca
    14521 acgaagctgg gataaaattg gaagtactga atcctcttga aagtcttaca aaagaacaaa
    14581 tggataatgg tgaagattat gtttcagtaa tggaagacaa tctaaaagcg ttagaaaaaa
    14641 caactatggt tgctggtgaa gaggtagttc cagaaaaaga agcgaaggat gaaaagacag
    14701 ttgctaatgg gtactttaga gatgccgatg tgaaggatcg cgaattgagt gattatacag
    14761 gcgaatggca atctgtttat ccgttgttaa aagatggaat tttggatgaa gttttcgatt
    14821 acaaagcaaa aatcaataaa gacatgacag ctgccgagta caaagattat tatactactg
    14881 gctataagac agatattgat actatcaaca ttaaagacaa caccattgat tttgtagtca
    14941 acggtgaaca tcatcaatac acatacaaat atgttggata caaaattttg aattatgaaa
    15001 aaggaaaccg tggtgttcgt tttaattttg aaactgatga tgcaggtgct ggtcgtttta
    15061 aatatattca atttagtgat catggaattg caccaagtaa ggcagagcat ttccatattt
    15121 tctttggtgg agaaagtcaa gaaaaactct ataatgaatt gcataattgg cctactttct
    15181 atccagcttc attgtctaaa catgaaattg ctcaagaaat gatggctcat taaaattcaa
    15241 tttttggagg aaatacatgg taagaaaagt attattgtct atggattcca agaatattga
    15301 tgcttatcgg cgttatcgat ttgcaaagca aatgagatta aatgggagta cattagctga
    15361 tattgctgca actttacatg ttagtattga cacagttcat agagtattaa aacgggatcc
    15421 tgattgttgt tttatcaagg tcaatactgg aagaagtaga cgtttagcaa aacggcataa
    15481 tagcttggcc aattaattga taataccaga gtagatcatt tttcccttat ttggatttga
    15541 tctgcttttt attatgtctt gaattgtcaa atcaagcgca acactttatc aaagaagact
    15601 tcataaggtg ttctccaatc taaacacttt ctaggtctgt tattcagttt ttcaatcatc
    15661 tgatttatcg ttgagttatc taccgtagct atctctttcg attttggcaa gtattctcga
    15721 atgagtctat tcgtatttta atttgttcat tgttgccaag ggcatgagga tctggaaaat
    15781 aaaagggcat attattcaat gcttctgtaa ctgcgaaatg attggagaat tcttttcctc
    15841 tatctggagt gatcgttttg accgcctcat ttggaatact gcttaaaagt tcaatcattt
    15901 tctcgctcac ataagcggct ttctttctgg ggaccttctt tcctgctaaa aggaagcatg
    15961 attttctgtc tgtcaaagtc actaaacaag taccaccagt tttgccagct acaatatcgg
    16021 ctttccaatg accaaaatca cttctatcat ctgcggattt tggacggcca tggatcgaat
    16081 gagtaaggca aatctttcca cgagactcga catgattttt tgtatgtctt gtttttcctc
    16141 gatggtgcaa tgaacggatc aggcctttat tgccgtgtga gaggggttct gtatcgaaaa
    16201 gatcattgca aattgcacgt tagatcgtga taaaactaat tgaaaagtcc atatgttcat
    16261 gcttcaaacg attcgagatt tcctctggcg accactgaaa atcgacaaat aaatgacgaa
    16321 caattgacca aactcacgga tcatttagaa tgtgataacg tccgcatttt tttcttcgat
    16381 aagcataatt tttttccgct ttggaaggtg aatagttttt ttcttgtttc ttctcagttc
    16441 atgtaaaata gtaaacgggc tatgctgaat gtcgttgcca attatactaa tagaaaatcc
    16501 ttcttcgtga agcagaaaga ttctttctcg ttcgagtata gtaagatgac gatactggct
    16561 cataattatt ttccttctag gagtgttctc gacaaactta tactagttgg atatcgatta
    16621 tgactcattt ttatgtgttg cacttaaagt gtaaattcaa ggtatagaaa gaggttccct
    16681 atgaaaaaaa tacctattac aatcttaact ggctatcttg gttcaggaaa aacgatactt
    16741 ctaaatcata ttctgtacgg tgatcacgga aaggaaattg ctgtcattgt aaatgacata
    16801 ggggaagtaa atgttgatgg ggtattagtc gacaagaaag gttttaaaag aactggagaa
    16861 aaacttgttt cgatgccgaa tggatgcatt tgttgtacat tacgcgaaga tttgattgtt
    16921 gaactagatc aattagtgca attttctgac ttgaatcaaa ttgttattga agcttcagga
    16981 atcagcaaac caattccaat tattcaagca attatattag ctaaaaaccc tttagacatt
    17041 gatttgaccc aacgattaga gattggtgcc attattatag tagtggatgt aaaacgtttt
    17101 tgggataatt tcgaaacggg tgaaaagatt caggaaagaa aagctgaagg taatgtagtt
    17161 gaagaagaac aggatattcg tggcttgtta attgatcaat tcgagtgttg taacatatta
    17221 cttttaaata atagtgactt agtgtcaacc aagacgattt acaaaattaa cggtctaatt
    17281 gctaagttac agtccaatgc caaaataatt aggacaatta aaggtgaagt agacttatcg
    17341 aaaattctaa atacaaatct ttttaatata gatgagactt cctattcagt gggctggttc
    17401 aaagagttag agcttggcta tcaaaatcat attcctgaaa cagaagaata tggtatctca
    17461 agttttatct atcgaaatcg gttactcctt gacgcacaaa aatttgccca tcttacggat
    17521 aaaaattttc ctggcaatat tacaagagca aaagggtttc tttggtttgc ccaacatagt
    17581 gatttttcga cgatctttga aacatcgggt agctataaaa gtattgaatc attaaattat
    17641 tgggtagcta gtttacctaa aattgagtgt caaatgatta tacaaaaaaa tcccgaattg
    17701 aaggaagcct ggcatccgtt gtttggtgaa cgcattaata aactagtttt tattggtatg
    17761 aagatggatc aacaatgtat taaggaacaa cttgagggtt gtttaactga ttctcttaat
    17821 gcagataatt tccgaaaaat ttcaccattt ccatggaaaa agtctaatat gtttagccag
    17881 atatcgttaa tgaaattttt aagaaaagct ggtgtcaaga taggcgatgt cataattatt
    17941 ggcattttga ttattacctc ttttattccc ttatttgtat tctctataga aaaggagaat
    18001 gaaacaactg aagatgctat caaatatgcc atcgttcgaa ttgatggcaa ggaaatggat
    18061 cgcttcgatt tggataagat caatcataaa ctcgtgaatt atcatcctgc gaagggacaa
    18121 tataatattg ttgagattaa agatggaaga attcgagtta aagaagataa tagtcctgat
    18181 caaatcgcgg ttagaactgg atggatctca aaacccggtc aaacgagtat ttgtttaccc
    18241 cataagttag ttattagtat tgagaaaaaa gaaagcacag attactacat ttactaatct
    18301 ggaaatagat gaggaggttt ttaataagaa aaagatatta ttattaataa tagcaggggc
    18361 tttattaagt gtgttaagcg cttgtggaca agaaaaagaa gcggtgacaa caggcacaag
    18421 tatctttaac gggacctgca atggtttagt taacagtcgg ttagattact tattttggct
    18481 cttcgtcaat gagtgttggt gaagaaattc aatagaataa tcgagatact cacacggcta
    18541 gacagctatc ttgtctagcc gtgttgttgt tctggcttta gtcagttggg tagtattctt
    18601 agttttctta aaatattttc ctcgatataa aaagattcgt tctcggtagt tttgaaagtt
    18661 tctatagcca taggcaattc gttttagtac tttgatatgg ttattcaaac attctatagg
    18721 gccgttggaa tagggaacgc gcaacgctcg tttgatttct gtttggtatt tcttgaaggt
    18781 cgtgaacact ggttggtagt gctctggcaa ctccttgtag tcttctttta ggagcgcgac
    18841 gaattcagga acatcttgtc ttctgataac ggaaagaaaa tcctggtaga cttcgtagcc
    18901 tcgctttaag gaatcatcat aagataatag ccgatcgact atctctgctt ctgtcagatg
    18961 atctcgaaat gacggacgcc agtagcgatg ctgacctttc aattcccacg cattcttttg
    19021 aagtagtttc cagtatttct ttaagtgctt cgaccgacga tccccttttt taaaggtctt
    19081 cattacttga attcggtggt tggtaaaagc ccgaccaatg tgttggctaa tatgaaagcg
    19141 atcggcgacg attcgggcat tcggaaaaca ctctttcacc aatgtgcgat agggtgtata
    19201 gaaatctgaa accactagac gcacttgttc acggactttt atagggtagc gcaagaaata
    19261 gtcgcgtaaa ggattgagtt gccgattttc cacaatatca atcagttgat gggtcttgcc
    19321 gtccatcatg acaaagctca tggctcccga gacatttttc actgatttga attcatcgaa
    19381 acacaccact tctggtaaca gctggctcaa agaaatggtt ttaggccgaa ggctgcgaag
    19441 tattcgaatg acagtggtcg gagaaatgtg cttcatacgt gcgatcaaag acatagaaag
    19501 aggttcgctt aatagctcaa gaatggcttg tttgactctg cgagcaatgg agcagcgacg
    19561 atcactaact gaatcttctg ctaaaaaagt ctttccacag tgtttacatt tgaagcgctg
    19621 tttgttaatc cgcagaatgg tcttgtagtt actgacatca ttcaataaga tggatacttt
    19681 tttccagccc tatttaatga tcgtttgttc attttgatag tggcgatgaa agcattcctc
    19741 cggttgatag gtcaatgtgc catcaaaagc aaaacatgtt tcgtcattga tgcgctcttt
    19801 gcgaaaggca tcctcatgaa aggtaagatt taggtctaga atatctagta actctttggt
    19861 aaactctgaa taggacatct tgaaacacta ctttgaatgg tttttgtcga ctttattcta
    19921 aaggatttta aggtgtctgt ttgcgtttat tagaaaaaag aaaaagtatt ggcaacagaa
    19981 ggcgtcacca acactagata ttatagatcc cttattttct tcgcttcaaa ttgtcaagga
    20041 gtcatcataa attttgtatg gattcttttg tttgggtagt agtcttctaa ataatcttgg
    20101 attaacacct tcatctctat tttactttta tatttgttgg agtcgattaa ttctcatttg
    20161 attgatcgat gaaacgcttc aattactgca ttgtcaacag gagtcccagt ttggctcact
    20221 gaatgtgtga tgtggtggtc gttcaacagc tcttgataaa taaaagaccg atgctggcta
    20281 cctctatcgg aatgaattac cgtaggcaat tctccttaat aaatttgtaa tggacggacg
    20341 acaatctctt tttttatgtg tgaatctacc atatagtaac gtagaatacg agttgctaag
    20401 tcaatatagg tggacaaata gaggcgaccg tcattacatg gaatgtaagt aatatccatc
    20461 acccaaacag catcaatttg gttctgttca aattatcgat tcaacatttt tttaccattt
    20521 ctttgattgt tttttctcta ccatgagaag atttgcgtcg atgaaatcct cttgtgcata
    20581 aattcatttc acgcataaga acagctacaa cacgtttatt gatttcgatt ccttcatcgt
    20641 aaagtttttg agtgatccct ggagatccgt atcgatcttt gtgttcgtag aacactcgtc
    20701 ttcctctttt agtcgtttat tttcctgttc aagaatgtag aaatctgctt tatcaggtaa
    20761 tctttttcct tgtccagcaa aagcattttt cccgtatcga cgatattctg aaacccatcg
    20821 ggaaattgtt ggacgagaga tattgtattc ttcagcaatt ttcttggtag ttactttttt
    20881 ctcaaggata tcctgtgaaa tctttagttt aaatttacga tcatatgttt ttctcaatag
    20941 agttcattcc ttccggtaac gtaactgata gtcaagaaaa gtgtatcata gcccaactta
    21001 gtatcagggt ttgtcaacta aactgtggaa gttgatagat aagagttttt aacaaccaaa
    21061 attctcttga ctatttttga aatcagacaa attttgattg gacacatagt tgaataaatc
    21121 taccgtcgtt acggaaggta aatcgcatgc ttttcaattc tagaggagaa gtcctcgttg
    21181 aagtaccata gcccagttct ggattcgacc tcctgcccac ttcatacgta aataaagtaa
    21241 ttttaggagt gagactgtga caaaagttat ttttaagcta aaaacgaatt aaaaaacgtt
    21301 gataattaaa atcattcgtt ttagattcta tgaatcatta tagaacgctg taaaatcgat
    21361 actttataat tataaagaag atagtttcaa atttattgtt cgaaatttga aactatcttt
    21421 ttacttatgt cacagactcc cttagttgag ttattcttta atttcgaatt tcgagatagc
    21481 tagatatatt gcaagcatac attcttgata acaatcttga tcatattttc cagttctcca
    21541 ggattgctta tgaatcaaag agctaaatct tttgagaatt gcctcaacag cttcttcatt
    21601 tccagccttt gctaaaatga aatcttcttt taaagaattc ataaatctac cttcttccca
    21661 tggacttata atctatttct ttgggaagga ggagaacgag caaagaagca tacaaaatta
    21721 gttattatgc agatattaaa ccagcatttt ttgaattttt ttgtaaagtc gatgttttaa
    21781 atttgtcgca ccctgtcttg taatccccag aacctttcca atttctttat cagttttatc
    21841 aaagataaat ttttcgataa gaaaaatttg ttcaggttct ttcaacaaag gaaggatttt
    21901 tgctaatttt atatcttcaa caaggagagt cacatttaaa acattaatcg gttgtttaaa
    21961 ttttatagta tctttagtaa acaaatatgg tgggagttga tcaagttgtt catgtttttg
    22021 aatttcttct tgttttttat aataagtatt agcggtgttc aaaatgacct tttcaaagaa
    22081 tcgaatcaac tgggcattta cgcgttcgtc gttatctgac atattgcaca cctcctccat
    22141 taagatttaa agggaaaaga ggcaaaaaac acgaaaagaa gctaatgcaa ttatgctgcg
    22201 ctgcgaataa cttctataag ctcacatgca ttgataggac tgctgatgtt tattttcaaa
    22261 aatgtttttt tagttgataa tggatagtta tctttatact tagaaaagaa aattcatata
    22321 tcttcttaaa tatcaaacat gtccttatgg ctaagaaaat gtcttgagct ttttacgtat
    22381 aataaatctt ttctaaaacc tccttttgga taatattctt attcgtgtat agattgtatt
    22441 ttttatttct tcccccttca taattaacaa ctagaaaaga gttcgaatag acacgtattt
    22501 gaacgaaaat ttttattgga ttcccaaatt ggaatattta gaaacgaaaa attcctatta
    22561 tctgtatagt gcttagataa taacggatca taataattat tgtctttgaa ctagatatat
    22621 gtaaatagta ttcatcatga tgaaaaatga tgagttctcg gtgataaaat gtacttttta
    22681 cggtggaata tacatgaaag cgataagtgg atattttata atgaagtaag ttagcttatt
    22741 ttgacagagg aatagtattt tagataacgt ataaaatact atttaatgat gtagaaagga
    22801 aagagagaaa caaaagtgtt ttcagataaa gaagtcttat taaagtctct tcatgtttgt
    22861 ttaaagttac ctatcgtggt ttttaattcc gaattcagta ttataaaaga gtatcgctca
    22921 gatagaacta cttatttttt ttatgattat aaaaagttac taactgataa tattaaaaaa
    22981 actaaagatt tccatttctt tactggtgat tttaatgaga tgtttcttct atacatgttt
    23041 aaaaccagat attacttatt tggtccattt agagcaaata atattgataa agtttttttt
    23101 aaactaaaaa tgaacaattt gaatgttgca atggctgata gagaacgact ctataattca
    23161 ttgcaaaatt tgactttgta ttctttagga gacattcgag atattctgat tttagttcat
    23221 tattttttta ctggaaaaat agaagatcta ttccatgaac cgttaattga atatacagaa
    23281 aatctaagta aaactatcga gcaaatcaaa atagataatc tgttatcaca aaattacgat
    23341 ccagaaatat atctgtttct ttatgaaaat aaaatactgg aatatgttaa aaatggagat
    23401 attcgaaatt tagaaaacat ggtttttaat ctgagtaatg gaataattcc aagtgttagt
    23461 ggagatacga ttcggtctga aaaaaattat tctatcattg tttttgaaaa gttagcacaa
    23521 acgtctataa cattaggaat ggatataata gaagcgtatc aaagtagaga tgctttgata
    23581 caagaaaacg aattagctgt atcgttacct gaggtcttaa aagtgaggga ttctgggatt
    23641 gtttattata ctaaggaaat cggaaaaact aagatagagc atctttctcc cttaatttct
    23701 tctgtcgttc aattcatcgg cttaaatatc tataaaagaa ttactgtaaa ggaaatagct
    23761 aattattttt ctgtaagtga aactaaatta agagaaagct ttaagaatga aatgcatatt
    23821 acgatttata attatatctc taagcgaaaa atttctacgg ctaaaataat gttgaaatca
    23881 aaccatacta tcagtgaagt ttctttaggt ttaggatttt cagattcttc acacttttcg
    23941 agagtattca aaaagtacgc aggtgtatct cctaagcaat atcaattagg tttagtggac
    24001 aatttcaaca acattagctg agaggtttta ttttattatg agactatatc atttacgtag
    24061 tttggaggag ttgaggaaat tggattttaa caaaaaattt ttttgggaaa tttctgattt
    24121 gagttgtttc gtagaatctg agcgtctaag taatggtaat aatagtgtgt taaatgaata
    24181 tgacaaagta gtaattaata aagtaaaaag tttatgtgaa gaagctagag aatctatcag
    24241 tcatattaat ggtagcaatt ttaaacaaac attatttaat ctgattaagc tagatgctaa
    24301 aatatcaagt tatttattct ttttgataca tgatgaattt atggattatc aaaaattgga
    24361 tcagttaatt gaaagagaaa gctgggaaga ttattacgtg ggtctaactt tttcagaaaa
    24421 gttaaacaac tacaagttaa tagattataa gtatttgagt gaaagcaatt aattattcat
    24481 gtagagtact agacacttta aatatggaca attcaattat aaagaaaaag aatcaataat
    24541 ttaatgaggc tgtgacaaat gtcatttttt gtttcgctaa aaacgagtga aaaaatgttg
    24601 ataactccaa tcattcgttt aagattctag gaatcattca gaaatgctgt gaaaccgata
    24661 ctttataatt ataaagaagg tgatttcaaa ttccgaagaa ggaatttgaa gtcacctttt
    24721 tacttatgtc acagtctcat tcagtaatgc gggataaggc tccttattcc gttatttgaa
    24781 tactctgaag tttaatctaa gaaagggtaa tattttgaaa acatgcttca tcgcatagaa
    24841 atgaacttaa tttcagaaag aatacataca tatcttttta tatgttttca gctgctgaat
    24901 ggttaaaaag cacaaatatc cgaaaaaaac atgaagaaat gtactttttt tgttgtaatg
    24961 ttcatgaaat tctctaagta aattagttaa cataaggtta gtaagattcc tattctgtga
    25021 ataatacgta tcagatttat cttttattct tactaggaaa gtaattatta agggagttga
    25081 tttgaataat ggttagcaag aataataaga gagtatttct agaaaaaaca aagaaacgag
    25141 tattgaagta cagtatcaaa aaattaagtg ttggggttgc atctgttttg attggtgttg
    25201 gtcttgtctt gggaacaact gaactagtta aagcacaaga tgaaatttct ccaagcagcc
    25261 cacttgaaac agcgataagc tccgtacaag taggtgacaa agtagcttcg gggaatactt
    25321 ttcaggagaa tcctggttat acgaaaaatt ataacttttc ggatttacaa tttagtcctc
    25381 aagaactaac tggagacaca ctaaaaggca atacaattgg atttgaagtt tacggaaaac
    25441 ataatattgc agcatcaact aaaaactggg aaatccgtct tcaattagat gaacgattag
    25501 ctaaatatgt agaaaaaatt caggtcgatc caaagaaagg aattggatct tctagacgga
    25561 cctttgtaag aattaatgat tcgctgggta gacctacaaa tatttggaag gtcaattata
    25621 ttcgtgcaag tgatggatta tttgctgggg cagaaacaac cgatacgcaa accgctccta
    25681 atggggtaat tacgtttgaa aagagcctag atgaaatttt taaagagata ggcatagaca
    25741 atctcaagac cgatcgttta atgtatcgaa tctacttggt aagtcatcaa gacgatgata
    25801 aaattgtacc gggtattgat agtacgggct actttttaac agattcagat gatttctata
    25861 atagcttaga tgtatctgaa aataatcctg accagtttaa acatggatca gtaagtgcaa
    25921 aatatgagaa acctaatact caaacaaaag atggttcagg atccacaggt gctaatggag
    25981 ctataatctt agatcataag ttaacgaaaa attataattt ctcatattca tcttccgcaa
    26041 agggcacgcc ttggtatgct aattacaaaa ttgatgaacg attagttcca tatgtagcgg
    26101 gtatacagat gcacatggtt caggctgaca aagtaacata tgatgtttcg tttgaatccg
    26161 gaacaaaagt agctgattta gcaatcgagc gacgggaagg tcatgaaaat tatggcatag
    26221 gttcaataac taataatgat ttaactaaac ttatcgattt tgctaatgca agtccacgtc
    26281 cagtcgttat tcgatatgtt ttacaattga ctaagccatt agatgaaatc ttagaagata
    26341 tgaaagcaac agcacaagtt gaagaaaata aaccatttgg tgaagatttc atctttgatt
    26401 cttggttgtc ggatacgaat aaaaaattaa tccagaacac ttatggaaca ggttattatt
    26461 atttgcaaga tattgatggt gatggaaacc ctgacgataa agaagagagc ggagacacga
    26521 atccatatat cgggaaacct gaattagaag aagtatatga tgttgacaca acagttaagg
    26581 ggaaagtatt catccacgag ttagcgggaa caggtcacaa agcccaactt gttgataaag
    26641 aaggtactgt attagcagaa aaaactatcg ctccaaatga aaaagatggg gctccaattt
    26701 cagatactgt agaatttgaa tttacgggtg tagattcaag taaattaatc gcgaaagatg
    26761 aattaaaaat ccaaatcgtt tctccaggtt ttgataaacc agaagaaggt tcaaccgtta
    26821 ttaaggaatc accaaaagcg gttgataaac aaaccgtggt agttggattt aaaccagatg
    26881 ctaaagaatc aattcggaat aataaaaact tacctgaaga tgcagagtat tcatggaaaa
    26941 cagagcctga tacttctaac gttactgata gtacgaaagg tattgtaact gttaagatcg
    27001 gaaatcgaac tttcgacgtg gatgtagagt ttgctgtaaa agcttctcaa gctatggaaa
    27061 atgatgcaac atacgtacct ataacaacaa ctccagaaac gacagttcaa agtggaaaac
    27121 ctacatttga taaaccagat gttcctttag ctaaagatgc cttttcaatt tcagatgtct
    27181 ataataagga cttcggtaat gcaagtgttg atgcaaacac tggtattgtt acattcactc
    27241 cagctaaagg tgtaggagaa tcggagccga ttactggaac aattcctatt aaaattgttt
    27301 accaagatgg ttctgtaggc acgaccgatt tagcagtaac tgtaagtaaa gatatttatg
    27361 aaaatccagg agaaaacatt cctgcaggct accacaaagt aaccttcacc gcagg
//
LOCUS       DAFEOM010000030        27078 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.031, whole genome shotgun sequence.
ACCESSION   DAFEOM010000030
VERSION     DAFEOM010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.031"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            244..1248
                     /locus_tag="LP524_001488"
     CDS             244..1248
                     /locus_tag="LP524_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2234547.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1249..2193
                     /locus_tag="LP524_001489"
     CDS             1249..2193
                     /locus_tag="LP524_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2234548.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2193..3155
                     /locus_tag="LP524_001490"
     CDS             2193..3155
                     /locus_tag="LP524_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2234549.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3182..4096
                     /locus_tag="LP524_001491"
     CDS             3182..4096
                     /locus_tag="LP524_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2234550.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            4122..5903
                     /locus_tag="LP524_001492"
     CDS             4122..5903
                     /locus_tag="LP524_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL2234551.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6251..6937
                     /locus_tag="LP524_001493"
     CDS             6251..6937
                     /locus_tag="LP524_001493"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBL2234552.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6957..10538
                     /gene="smc"
                     /locus_tag="LP524_001494"
     CDS             6957..10538
                     /gene="smc"
                     /locus_tag="LP524_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBL2234553.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10547..11356
                     /locus_tag="LP524_001495"
     CDS             10547..11356
                     /locus_tag="LP524_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL2234554.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11367..12365
                     /gene="ftsY"
                     /locus_tag="LP524_001496"
     CDS             11367..12365
                     /gene="ftsY"
                     /locus_tag="LP524_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBL2234555.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12552..14819
                     /gene="gshAB"
                     /locus_tag="LP524_001497"
     CDS             12552..14819
                     /gene="gshAB"
                     /locus_tag="LP524_001497"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HBL2234556.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(14908..15360)
                     /locus_tag="LP524_001498"
     CDS             complement(14908..15360)
                     /locus_tag="LP524_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HBL2234557.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKSNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            15510..16460
                     /locus_tag="LP524_001499"
     CDS             15510..16460
                     /locus_tag="LP524_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBL2234558.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            16453..17412
                     /locus_tag="LP524_001500"
     CDS             16453..17412
                     /locus_tag="LP524_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBL2234559.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            17409..18164
                     /locus_tag="LP524_001501"
     CDS             17409..18164
                     /locus_tag="LP524_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL2234560.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            18187..19140
                     /locus_tag="LP524_001502"
     CDS             18187..19140
                     /locus_tag="LP524_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL2234561.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(19784..20080)
                     /locus_tag="LP524_001503"
     CDS             complement(19784..20080)
                     /locus_tag="LP524_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HBL2234562.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            20435..20791
                     /locus_tag="LP524_001504"
     CDS             20435..20791
                     /locus_tag="LP524_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBL2234563.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            20801..21700
                     /locus_tag="LP524_001505"
     CDS             20801..21700
                     /locus_tag="LP524_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HBL2234564.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            22217..22948
                     /locus_tag="LP524_001506"
     CDS             22217..22948
                     /locus_tag="LP524_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBL2234565.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            23093..25120
                     /locus_tag="LP524_001507"
     CDS             23093..25120
                     /locus_tag="LP524_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBL2234566.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            25228..26325
                     /locus_tag="LP524_001508"
     CDS             25228..26325
                     /locus_tag="LP524_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBL2234567.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            26632..>27078
                     /locus_tag="LP524_001509"
     CDS             26632..>27078
                     /locus_tag="LP524_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2234568.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT         9079 a         4683 c         5706 g         7610 t
ORIGIN      
        1 aaaaagctgc atgcagcttt ttatccttct ttttttaatc tttttgacac gtaaaagtca
       61 tttttctgat aattctggcg ataaacagaa attatgttgc aaagtaaagc ggttttctat
      121 ataattatta ataattcatt acacaaagat aagaataacc tttgttataa aacgatttat
      181 tctgtgtaat gtcctttttt attaagaaaa acttccattt aggaagcaga gaggggtatt
      241 cgattgtccg ataaccaatt attagaagtt cagaacctac atacaggatt ccgactcaaa
      301 gatgactatt acgatgcagt agatgacgtt tcatttactt tagacaaaaa cgagatttta
      361 gcaatcgttg gggaatctgg gtgcgggaaa agtaccttgg ctactacgat tatgggatta
      421 cttgatccta ataacacaaa aattacaggt gagattttat ataacgatct gaatttaatc
      481 gacctgaacg aaacgctcta taataaaatc cgcggaaatg atatcggaat gattttccaa
      541 gatccgttgt ctgcattaaa tcctctcatg agaatcgaag accagatcaa agaaggactt
      601 tcctatcaca caaagatgaa tgcagaacag cgtcaagctc gtgcactaga attgctagaa
      661 caagtaggga ttcctaatcc tgcgagagtt ggacgccaat atccccatga actatcaggc
      721 ggtatgagac aacgtgtcat catcgcaatc gcaatcgcct gcaaacctcc aatcgtcatt
      781 gcagatgaac cgacgacagc cctagatgta accatccaag cacagattct tgatttactg
      841 aaagaccttc aggaagaaac aaaaacaggg atcatcttga tcactcatga cttaggagtc
      901 gtagcagaaa tggctgatcg agtggcagtg atgtatggcg gacagttcgt tgaagtagca
      961 ggtgtaaaag aactgtttga tcatcctaag catccttata cacgctcttt actgaactct
     1021 ataccacaag aaggaaatca tgatgcagaa ctccatgtga tcgaaggtgt cgttccttct
     1081 ctgaaaaaca tgcctcgtaa aggttgtcgg tttgcaccac gtattccatg gataccagca
     1141 agcgatcatg aagaagagcc tatccttcat gaagtcgcac cgaaccactt ggtacgttgc
     1201 tcctgctata agcatttcca ctttagagac gaaaaaggag aggtgtagat gacagaatta
     1261 atcacaatca aagacctgaa agttcattac cctattcgca gtggattctt caatcgagta
     1321 acggatcatg tatatgctgt agatggtgtg gattttatta tcgaaaaagg aaagacatat
     1381 ggtttagtgg gagagtctgg ctctggaaaa tctactacgg gaaaagccgt tgtgggatta
     1441 gaaaaggtaa cagccggaca aatcatgtat gaaggaaaag atgtaacgaa gcgaagcaac
     1501 cgcaaaaaaa tgggttataa caaggatgtg cagatgatct tccaagattc aatgtccagt
     1561 ttgaatccta aaaagcgagt gttggatatc attgctgaac ccatccgaaa ttttgaacgg
     1621 ttaagcgatc aagaagaaaa gaaaaaggta aaaggactgc tagatatcgt tgggatgccg
     1681 gaagatgcgc tatataaata tcctcatgaa ttttcaggcg gacaacgtca acgtctagga
     1741 gtagcccgtg ctgttgctac caatccaaaa ctgatcgtag ccgatgaacc tgtatctgct
     1801 ctggatctat ctgttcaagc gcaagtattg aatttcatga aacgaatcca gcaagaatac
     1861 ggcctcagct atttattcat ttcacatgat ttaggtgtag taaaacacat gtgcgacaat
     1921 atcgcaatta tgtacaaagg tcgatttgta gaaatcggta caagagaaga catttataat
     1981 gacccgcgac atatctatac gaaacgtctg ctttcagcta ttccgcaaat cgatgtagaa
     2041 aatagggaaa gccacaaaga aaatcgacga agagtagaac aagagtatat ccaaaaccaa
     2101 aaagaatact atgatgcgac tggtcgagtc tacgatttgc gtacgatcac aactacacac
     2161 aaagtggcgt tgaaagatgg aggtgcaagc taatgtggaa aacgattctg cgacgtgttt
     2221 tattgatgat cccgcaagtt attattttaa gtgttctgat cttcttatta gcccaagcga
     2281 tgccggggga tccatttacc ggtttgatca atccaaatca ggatccggca gtgatcgaac
     2341 agatgcgaca agcagctggc ttgaatgacc catggtatgt ccagtacttc cgttggatcg
     2401 gaaatgcttt gcaaggcgat tttggtcaaa gtttcttata caaacgctct gttgctgact
     2461 tgatcggcga acggatcatc aacactttat atctatcgat cttgacagtt gtccttactt
     2521 atttgattgc tgtgcctctg gggatgcttg ctggccggta tcaaaattca atcttggata
     2581 agattgtcgt aatctacaac ttctttagtt ttgctgttcc tttatttatc ttcggcttga
     2641 tcatgctatt tgtttttggt tatcgtttag gctggttccc aacaagtgga tcacaaacac
     2701 taggtttgca aggtggattt atcactcaat ggatagaccg tttgaaatat attctgttac
     2761 catcaattac ccaagcattt ttggcgactg ccgtcacaat ccaatatctg cgaagtgaag
     2821 taatcgattc gaaatcgctc gattttgtca gaacagcccg ttccaaaggg gtaccgacga
     2881 ataaagtatt cagtcgacat attttccgaa atgcagcgtt gccgatggct tctcaaatgg
     2941 ggtatgaaat cacttcgttg attgctggtt ctgtagttat cgaaaagatt tttggttatc
     3001 cgggagtcgg aaaactattt attgattcca tcggccaacg ggactatacc gtgatcacat
     3061 ccttagtatt gattttagga atcgcgacgc tagtggggac attactttca gatatcatca
     3121 tgagtatcgt tgacccgcgt attcggattc aataaaacca atcagaaagg agaatggatc
     3181 gatgagtgaa aaaaacaata cagcagttca agaaagtatt cctcctatgg ggatacgaat
     3241 gattgctcga gaatttaaaa aagataaaat tgctattttt tcattaggtt tgctagtggt
     3301 tttattatta gtgatcttta tcggtgcgat gctgaccgat caagacaaag tgatgtatgt
     3361 tagtatcttc gacaaatacg ctgcaccagg aacagtttcg acacaaggac aaaagttcat
     3421 tctgggagca gatgaaggcg gacgtgatgt attaggacaa ttgatcatag gtgctagaaa
     3481 ctctgttacg atcggatttg cgatcacgat ccttacttcg atcatcgggg ttggattagg
     3541 gatcatctct ggctactacg gagggatcat tgacaacatc ttgatgcgga tcgttgattt
     3601 tatcatgatt ctaccgatca tgctgatcat tatcgtcttc gtatcgatta tttccagata
     3661 caatgtctgg tcctttatat tcatcatgag tgcattttat tgggtagcta aagcccgatt
     3721 attccgaagc aagacattat ctgaagcaag aagagattat gtcagtgcct caaaaacaat
     3781 gggaaccaat gattttaaaa tcatgttccg cgaaatcatg ccgaatctaa gttcattaat
     3841 cattactaat ttgacgatga actttgcggc aaacatcggt atcgagacaa ctttgacttt
     3901 cttaggtttc ggcttgccgg caaatgtacc tagtttaggt acattgatcg gttatgcaag
     3961 taacggagaa gtcttagtga atcggcaatg gatctggtta cccgcatcaa tccttatttt
     4021 agttttgatg ttgagtatca attatgttgg acaagcattc aagcgttctg cagatgcgcg
     4081 ccaacggtta ggctagtata gaagcgaagg gggaaatagg aatgaagaaa acgatttttg
     4141 gatttgcagc gctgatgtct gctgtcgttc ttgcagcatg cgggggcgga ggtacagata
     4201 cctctggaaa caactctgga ggcaacggca ataacaaaga ggaattaact tttccaattg
     4261 caacaaaaaa taatgatgat gcaatcgaag acggcgaatt agaagctgcg gtcgcaacgg
     4321 acagtcagtt taaaggattg ttccagtggg agttttacca agattcatat gatgcagctt
     4381 ttatggctcc ttctcatgaa ccgctatttg caaacgatga cgatttcaga atcacagacg
     4441 acggagctgc aacgcttgat ctagatgaag atacgaaaaa agcgacaatc aaagtaaaag
     4501 acaacgtcaa atggtcagat ggtgaagatg taacggcgga cgatgtgatt ttcccttacg
     4561 aaatcattgg gaataaagat tacactggta tccgttatga cgacactttc cgtaacatcg
     4621 taggtatgga agaatatcat gatggaagtg ccgacacaat ctccggaatc aaaaaaatag
     4681 acgacaaaac agttgaaatc gaatataaag aagtaaaccc aagcatgctt caatcgggtg
     4741 gtggtatctg gtcatatgca gcaccaaaac atacgttaga aggtgtagca atcaaagata
     4801 tggaatcaag cgatcaagta cgtaaaaatc cagtgacttt tggcccttat tacatgagta
     4861 atatcgttgc aggagaatca gtagaatact tgccaaacga gcactactgg aatggtacgc
     4921 ctaaattgaa aaagatcacg atgaaatctg taccgatggc aagtgcaact gaagcattga
     4981 actctaaatt atatgatatg attttccaaa tgccaacaga tacttacgat acctataaag
     5041 aaattgctgg ctatacaaac ttaggccgtg aacaaacttc ttatacttat ctaggattca
     5101 agttaggtaa atggaatgca gatacaaaga gtgtggaata caatccagat tcaaaaatgg
     5161 ctgataaatc attacgtcaa gcaatgggct atgcattaga taataatgca gtaggtgaac
     5221 gtttctatgc aggtttgaga agcaatgcga cttcattgat tcctccagta ttccagtcat
     5281 accatgatga tgagatgaaa ggattcacac aagatctaga caaagcgaaa aaactattag
     5341 atgatgctgg ctacaaagat gtggatgacg atggtttaag agaagacaaa gatggaaata
     5401 aactaacaat caactttgct tcgatgtccg gtggggaaac agcgcaacca ttggcagatt
     5461 actatatcca acaatggaaa gaaattggtt tggatgtcca atacacaaca ggtcgtctga
     5521 tcgaattcaa ttctttctat gataaattag aaaacgatga tcctgaaatt gatgtttacc
     5581 aagctgcttg gaatacagga actgatccaa atccaagtgg actatgggga ccaaatgctg
     5641 cgttcaacta cactcgttat gaatcagaag aaaatacaaa attgatcaac gatatcagtt
     5701 caagcaaagc atttgatgaa gagtaccaaa aagaagcata tcaaaaatgg caacaatatg
     5761 catttgaaga agcatttgcg attccaacgt tgttccgtaa tgaagtattg ccagtcaacg
     5821 accgtgtcaa agactgggat tggtcttatg atgtaccaaa cccatgggca aatgtttctg
     5881 ttacagcaga ttcacgttca taatcactaa aaatgaccga aaaagggcga tgtccttttt
     5941 cggttttttg atatttttct gtttatttct ctaatattca ggtcaaggcg ctgatttcat
     6001 gacttctcca ataaacggtg ttcaaagtcc agtttctcgg tattaagtca aatttatgca
     6061 aaaatatgga aagccatttt cacaaaattt tttcttcata ctcgaaacta aacgaatttt
     6121 tcacaacttc tcgtcaatca ctaggattat taaagcttta cttacctttt gaccgaacca
     6181 tttcaaattt gacaaaaaac cagtataata aggaagtatc gaaaaaatat ccataaaagg
     6241 aatgtggaaa atggacaacc agctgacaaa agaactaaaa gaaaactata acatcgtttt
     6301 tcacgatcta aatttgcttg aacaagcttt tacccattca tcctatgtga atgagcatcg
     6361 caatctgcaa ttatcggata atgagcgact tgaattttta ggagatgccg ttttagaact
     6421 gatggtttct cagtatttat tccgtttata tcctcatttg ccagaaggaa aattgacaaa
     6481 aacacgagct gcgatcgttc gcgaagacag cttgtcgaaa tttgccaaag aatgtcactt
     6541 tgatcaatat gttttgttgg gcaagggaga agagaattca ggaggacgta cgcgaccagc
     6601 attgctttgc gacctattcg aagctttttt aggcgcgtta tatcttgacc agggattcga
     6661 cacgacccat gcattcatcg aaaaagtgat ttttcctaaa gtcaaagcag gtgctttttc
     6721 acatgagatg gatcataaaa caaaactaca agaagtcttg caaaaatcag gtgatgtatc
     6781 aatcgaatac cgattgatca atgaagaagg tccagctcat gaacgagtat tttggatcga
     6841 agtttatgta gatgatcaat tgattggcac tggacaaggg aaatcgaaaa aattagcaga
     6901 acaagctgcg gcggaaaatg cattggcagc actgtaaaag taagtgaggg aaagctttgt
     6961 atttgaaacg aattgaaata gccggtttta aatcatttgc agacaagaca gtcattgatt
     7021 ttgaaaatag cgtcacagca gtggttggac caaatggcag cggaaaaagc aatattacag
     7081 aggctatccg ctgggtatta ggagaacaat ctgctaaaag tctgcgaggc ggaaaaatgc
     7141 cggatatcat ctttgccggt tccgataccc gtaagcagct gaatattgca gaagtcacag
     7201 tgattttaga caatagtgat cattatctgc cattggagta caatgagatc agtgtaaccc
     7261 gccgttatcg acggactggg gaaagcgagt ttttcatcaa taaacaaagc tgcagattaa
     7321 aagatatcca agaattgttc ttagattctg gattaggaaa agaatcgttc tcaatcattt
     7381 ctcaaggaaa agtagaagcg attttcagta gtaaaccgga agaccgtcgt gggatctttg
     7441 aagaagcagc tggtgtcttg aaatacaagc agcggaaaaa gaaagcagaa cagaagcttt
     7501 ttgaaacaga agacaacctc agccgcgtcc aagacatcat ccacgaattg gaagaacagt
     7561 taactccttt ggccgcccag agtgaggcag caaaggagtt tttacggcta aaagaaacac
     7621 tgactcaaac cgatgtctct ttgatggttg ctgaaatcaa gacagcaaaa aaagattggg
     7681 acaacaaaca agcacagctt gcgaaattca atctagaatt aggcaaacta tctgagtcga
     7741 tccaagaaca agaatccatt ttggcgaagc agcgtaaaga aaatgcgcaa gccgatcgtc
     7801 tgatcgaaaa aaatcagcaa gtattattag atctttcgga aaaactgaag cagacagaag
     7861 gacaaaaaga tgttttgcag gagcggacaa agcatacaca aaaaagctcg caagaatatc
     7921 aaacttcatt agcagaagca caaaaaaagg ttaaacattt tgaaaagctt caagaaagct
     7981 tgatgaaagc agcagcagaa aaagaaacag agatccaaaa agctgaagca aatctcataa
     8041 aaacacagca agaattagaa aaataccaaa aatcaacgaa agaattattg gcagaattaa
     8101 gagatcaata tgttgatttg atgcaagaac aagcagctgt aggaaacgaa ctgaagtatc
     8161 tagaacgtca atatatacaa gaaacagcaa aaagcaagca gacgttagcc aagcaatcgg
     8221 aagtagaagc atcagtagac cgattgatct tacaaaaaga agaattgaca caaaagcagg
     8281 cgcagctaaa atcatcttta actgaaacaa aagagaagtt agaaatgatc caacaaaacg
     8341 ggaaaaagtt ccaagaagca ttagcaaaag aacagcctaa aatgtatcaa ttaatgaatc
     8401 aagtccagca gctacgtgct cgtaagaaaa gccttcaaga aatccaagaa aactattttg
     8461 gtttttacca aggggttcga ttggtcttgc agcataaaca gcagctttcg ggtattgtcg
     8521 gagcagtggc tgaactgatc gatgttcctg cagatttcac gttagctatc gaaacagctt
     8581 taggtggggc agcccagcat gtcatcgttg aaaatgaaaa agatgctcgg caagccatca
     8641 cgtatttgaa acagcaaaga ggaggaagag caacattcct tccactgacg acaatcaaac
     8701 caagacaatt accggctcat atattgacac aagcagcagc tgtagaaggg tttataggga
     8761 ttgccagtga acaagtcagt tatcccgatc aaatccaaac tgtggttcaa aacctgctag
     8821 gaacgatcct tttagccaaa gacttaacta gtgccaatgc aatcgcgcaa acgatccgct
     8881 atcaatatcg agtggtttcc ttagaaggtg atgtgatgaa tgccggagga tccatgacag
     8941 gtggagcgaa taaacgagga aaccaaggca gtttgtttgt acaaaatcaa gaattgaaac
     9001 agttgactag tgaatttgaa gaagcagata aacaactcca agcacaagaa aaaaaagttc
     9061 aggagctgca acaagaaaca gctcgtttag ccgaagaaca agaagttttg cgtacacgag
     9121 gcgaacaatt acgttttgaa gaacaagaag cgacaaatca gctacaaaac atcataaatg
     9181 aacttgaacg ttttgaaaaa gaaaagcaaa tctcaacttt tgagacacgt gaactgcagc
     9241 agtttatcga agattacgaa aagcaaacga acgaattgaa agacaaacaa acagatcttg
     9301 aatcgcaacg acaaaaaatc gacgaagaaa tcaaatcgtt aagtcaagaa agcgaccaga
     9361 tggaagccag aagagcacaa gtacaaagtc aaaaagcaca agaacaagca gatcttgctg
     9421 tcttgaaaga gcagtttaat catctgcaga tccagctccg cggtgcccga gtccaaaaag
     9481 cagaggcaac cgaacgtcaa gaagcgatag aaaagcagtt ggcgacattg acagcagact
     9541 tttctgatca tgaagtcaca gaagaaagtt tggaaaaaca aattaacgaa ttatctgctc
     9601 agcgagaaac attaaaagcc gagttggcaa aagccaaaga gcagcgagat agaaggcaaa
     9661 aggagatcga taagcttgaa gcagtcttgg cagagcgtaa tcgtgagcaa aaagcacgac
     9721 tgtcggaaca atccaaatta gaagtccaaa aagaccgagc tgaaatgatt ttagataatc
     9781 atttgaccta tctgcagtca gaatatcagc tgacatttga gaaagcttcc caagactatc
     9841 aagaaacaac cgatatcgaa gacagtagaa caaaggtttc atccttaaaa gaacagattg
     9901 aaaaactggg accagtaaac ttaaattcaa tcgaacaata cgaacaagtc agtgaaagac
     9961 atacattctt agcaacacag cgagatgatc tgttagcagc gaaaaaccaa ttgtttgaaa
    10021 cgatggatga gatggatgat gaagtgcgga caagattcaa agaagtattt gaagctatcc
    10081 gtcaagaatt caaaattgtc tttccaaata tgtttggcgg cggtaaagct gaattagtac
    10141 tgacggaacc aactgatttg ctgaaaacag gtattgagat tgaagtacaa cctcctggca
    10201 aaaaactgca aagtctaagt ttgctttctg gaggagaacg agcacttaca gccatcgcgt
    10261 tgctcttttc cattatccgt gtctgtcctg ttccgttttg tatcttagat gaggtagaag
    10321 cagcgttaga tgaagccaac gtgaaacgtt ttggtcgtta cttgagcgat ttccaagatg
    10381 acacacaatt tatcgtagtc acacaccgca aaggaacgat ggtcgcagca gatgtactgt
    10441 acggagtgac gatgcaggaa tccggtgtat caaaaatcgt ttctgtacgg atggaagata
    10501 tcaatgaaga ggcgaaaatc aacgaaacga aagtgtagga acgaaaatga tcaaattagt
    10561 agcaatcgat ttggacggta cattattaaa tgaacaaaag ctgatttcag atgagaataa
    10621 acaagcatta gcacaagcaa aacagcaagg ggtcaagatc gttctatgta caggacgccc
    10681 cttagctgcg atggcgcatt atctgcaaga attaggactg gtagatgaag gtgactacag
    10741 catcactttc aacggcggtt tggtacaaaa aaatgatact ggtgaaatca tggaaaagaa
    10801 agtgatggaa gtatcagata tccaacgctt atacaaactg gctcagcaat tggatctgcc
    10861 actagatgtt ttgtctgaca atgtggtttt acagctgcct agtgccccaa ggaagcaatc
    10921 cttgtacaat gtgttgaata agctgctgca attccaacca gcggatataa gcgatattac
    10981 agatgaattt gtgcttaata aggcagtaat cgcttatgac caagaagaat tagatcctaa
    11041 aatcaaagaa ataccagcag aattccatga aatgtatgag atcatcaaaa caagaagtat
    11101 gcttttggaa tttatgccaa aaggtgttac aaaagcttat ggtatttctt tattggcaaa
    11161 agatcttggt ttgaaacaag aagaaatcat ggcgatcggt gatgaggaaa atgatttacc
    11221 gatgatccaa tatgctggac taggcgtagc gatggagaat gctgtgcctt tcgtcaaaga
    11281 agcagcagat ttcgtgacag ccagcaatga agaaaatggc gtagccaaag tgatccaaaa
    11341 atttgtatta gagtaggggg agtcaaatgg gattttttga taaaatcaag cgtgctttta
    11401 ctggcgaaga gaaaacagaa gaagttgaag tacaggagaa gtacgacaag ggtttagaaa
    11461 agacgcgtaa aacatttggt gaacggatga atgagttgtt tgccaatttt cgaacagtcg
    11521 atgaagactt ttttgaagaa ttagaagaaa cacttatcgg tgcagatgta ggttttgaaa
    11581 cagcgatacg aattaccgaa gcattacgtc aagaagtgaa actcagaaat gcgaaaaaat
    11641 cggcagaagt ccaaaatgcg atcatcgaga aacttgtgga tctctatgaa gcggaaggaa
    11701 tcaatgaagt aaatgaacta aatatccaag aagacggact aacagttatt ttgtttgtcg
    11761 gtgttaatgg tgtagggaaa acaacaagta ttggtaaact tgcccatcag ttcaaaaacg
    11821 aaggtaaaaa agtattgatg gcagcagcag ataccttccg cgctggagca atcgaccagt
    11881 tagttgtctg gggcgaacgc gctggtgttg aagttgttcg tggcaatgca ggaggcgatc
    11941 cagcagctgt cgtattcgat gctttggaaa gagcaaagac agagcaggca gatgttttac
    12001 tagttgatac tgcaggccga ctgcaaaata aagtgaactt gatgaacgaa ttagaaaaaa
    12061 taaaacgagt aatccagcgg gaattaccag aagcacccca agaaacactt cttgtattag
    12121 atgcaaccac tggtcaaaac gcgatggttc aagcaaaaca attcaaagaa acaacagatg
    12181 tgaccggtct tgttttgaca aaattagatg gtacagcaaa gggcggtatc gtacttgcta
    12241 tccgtaatga attgcattta ccagtgaaat tggtcggtct tggtgaagga atcgatgact
    12301 tggagccgtt tgatccaaat gattttgtcg ttggattgtt caaaggatta ctaaaagaag
    12361 aataacccat gtctaaaaag caggagctgt gacaaaagtt gcagctcctt gtttgaatga
    12421 ttgtttttaa catagctgtg tttttgatgc cttcattttt atacttattt gtaagcggtt
    12481 ctcaaacaat ttcgttcttt ttcttagact ttttgattat aattagattc atatgaattg
    12541 gaggtcactt aatgatgaat tttaagcaat tattattgca tgtcaatgcg cgccctttta
    12601 tcgaccaagc tcgatttggt atagaacggg aaggacagcg ggttgatctt gcaggaaatc
    12661 tagcaaaaac cgatcatcca gcaatctttg gcgatcgatc ctatcacccc tatatccaaa
    12721 cagattttag cgaaacacaa acagagatga tcacccctgt taccgattct attcccgaat
    12781 tatttcagta tctcgctgct gtttatgatg tgactgctcg ttctataccg aaagaagaga
    12841 tgatctggcc attaagtatg ccacctgcct taccggaaaa agacgaagag atcattattg
    12901 caaaattaaa aaatttcgaa gatgtcttgt atcgacgtta tttagcaaaa gaatacggga
    12961 agcgaaaaca gatggtaagc ggtattcatt tcaattttga atttggcgat gagttgctaa
    13021 gaacattgtt cagccatcag gaagaatttc aagatttttc cgaattcaaa acagaacttt
    13081 atttgaaaac agcgagaaac tttatgcgct atcgctggat gatcacttat ttattcggtg
    13141 cttcaccaat gagtgagaag aactactttt tagatgaatc acatccgcaa gaacctgttc
    13201 gcagtattcg caacagtgcg ctgggctata cgaatcatcc aaatgtgaaa gtttcctatg
    13261 cttctatgaa acagtattta gcagatatcg agcgaatgat cgaagaaggg aaactttcgg
    13321 aggagaaaga attttataca ccgcttcgtt tccgaggcgg aaagaaagtc gcagatttag
    13381 caacaacagg tgttcgttat attgaattgc ggaatatcga tttgaatcct tatgcaagat
    13441 tgggtatcaa tccagagcaa gttcggttct tacaattatt cctgatgtac atgttatgga
    13501 cagaagaaaa agaagactgt gaccaatggg tagcagaagg tacgactcga aataacaaag
    13561 tagcactaga acaaccatca gatcaaacag aatttcatca agaaggtaga gaaattcttg
    13621 aaggaatgaa gcaaatgctg gttgaattgg attggttgga ttctctttat ttagtagagg
    13681 aagcattgac tcaaatggat catcctgaac aaacgttagc agcgaaactc tatcaagaag
    13741 cacagctatc gagtcagcaa gaagttgctg tggcattggg acatcaatat tataaagaaa
    13801 gccatgaacg tccctatcag ttggccggtt ttcgcgaaat ggaactttct actcagatat
    13861 ttatgttcga tgccatccaa aaaggtgtcc aagtcaaagt attagatgag tcggatcaat
    13921 ttttgcgtct gcaattccaa gaccatgtag aatatgtgaa aaatgcaaac atgacaagta
    13981 aggacagcta tatcgtccca cttatcatgg aaaacaaaac agtaacgaaa aaagtcttaa
    14041 aagaagctgg gttccgggta ccaggaggcg cagaattttc atccatggaa gaagcggtaa
    14101 aagcttatcc gagatttgcc gagcaggcat ttgtgatcaa acctaaatca accaattacg
    14161 ggttaggtat cacgattttc aaggaaggag ctagtctgga agattatcaa gcaggtttag
    14221 ccattgcttt tcgtgaagac agttcggttt tagtagaaga atttatgccg ggaacggaat
    14281 atcgtttctt tgtgatcgac ggagaagtcc aagccatcat gttgcgagtc cctgctaatg
    14341 ttattggtga cagtatccga acagtgaaag aacttgttga agaaaaaaat agtgaccctt
    14401 tgcgtggaac gaatcatcgt gcacctttag aattgatcca attaggtgaa ttagaacaat
    14461 tgatgttgaa agaacaaggg ttgacgatcg aatcagttcc ccaagccaat caaattgttt
    14521 atctaaggga gaactcaaat atcagtacag gtggtgattc gatcgatatg actgatgaat
    14581 tttctgaagc ttacaaaaaa atcgccgttt ctgcggtaga agcactagga gctaagatta
    14641 gtggtatcga tttgattata ccagataaag aaatcgatcc tacaactgat aaaaaagcat
    14701 atgggatcat cgaagcaaat ttcaatccag ccatgcatat gcatgtttat ccttttgcag
    14761 gaaaaggcag aagattgacg atgaatgtat tgaaattgtt atatcctgaa gtattttaaa
    14821 agaaaaagct tttgacagat tgtcctattt gatgtcaatc tgatcaaaag ctttttttgc
    14881 atgtaaaaat ttatgtgttt tttcgtttta aaataaacga tcccagaagc tttttttctt
    14941 ttttggtttt tctttttcag ctggcgaaga gttttcttct gggtattttt tttcgatatc
    15001 aatcacaggt tcatttactg cttcttttgc tgttaagagc aatccgaatt cctcaggatt
    15061 tgcagtgacg taatcgttga ctacagtgaa acggctattg cttttcgttg ctagctggat
    15121 atattggttt tggacagaaa ggggtagagc cccattcaag agtatggaag catctggatg
    15181 ttttttgagt tcttttaaaa agttttcttt gttttttggt tggatcattt cagcaacagt
    15241 catactaaga tagcagcgtt ccctgaatgt ccctaaatat ttatgctgtt cttcagggtt
    15301 cactaaaggt gtaccataaa gtcccttatc caaatggctt tttacatcat cttgtgccat
    15361 aaaaaatacc ctccttcttc tctttgtctt tcttacgatt atagcatatt cccaataata
    15421 cctaaaacat atgaaaaagc ggttgctttt gttcaacgat tcacttaaaa taagaatgaa
    15481 aatcattctc aattagaaag gtggattgga tgcgctacgg acgactagta gcattattag
    15541 ttattttaag tagtatttca ttgttcatcg gagtatcgaa tgtacaattg tcagaactgc
    15601 tccagttaga tgggcaaagc ttacaaatct tgctagtgac tcgtttgcca cgaacgattt
    15661 gtttgatcct tgtgggagcg accagtagta tttgtgggtt gatcatgcaa catttgaccc
    15721 aaaataaatt tgtttcacca acgactgcgg gcacgatgga tagtgcgcgt ttaggtattt
    15781 tagtggctat gattttccta cctggtgcat ccttgattgt ccgatccttg acagcatttt
    15841 gctttgcttt tgcaggaaca ttgctttttt tatcccttac gcgattgttc ctccagaaaa
    15901 atcaattgat gcttccgttg attggagtca tgttagggaa tatcattggt tcagttgcta
    15961 ctttttttgc ttatcaattc cagttggtac aaaatatgac ttcatggctt caagggaatt
    16021 ttgcgactgt gatacgagga aattatgagt tattgtatgc tgttgttcca ttgttgctct
    16081 tggtttggct atttgcttac cgatttacga tcattggtct gggcgaatcg tttgcagtaa
    16141 atctgggaat agattaccag aagatgcagt ttttaggcat tggcttagta tcattggcta
    16201 gtgcagtcat gttgatcatg gtaggaagca ttccttttat cggagtgatc attccgaatc
    16261 ttgtctctag aatatatgga gatcaagtgc acaaaacaat tggcgtaact gctttagcag
    16321 gtagtttgtt cttagtattt tgcgatcttg tcgctcgcgt gttgattttt ccttatgaaa
    16381 taccagtcag tgtgatcgtt ggaatcatcg gtggggctat cttcctttat cttctgataa
    16441 ggggacgacg atatgcttaa aaaaaagcca tctattttta ttggtatgct tgttcttgtt
    16501 ttatttgtta caggaatgta cttgacgatc catacatatg gaaactggca ttttgcatgt
    16561 gcattgagga gtaagaaaat ttttgcattt atccttgtct cgctagctgt cagcagtgcc
    16621 acaatcagct ttcagacaat tacgcataac cagtttttga ctccttcagt attgggactt
    16681 gagtcgttat atgtccttgt ccaaacggtc cttttcttct ttatcggagg agtgacgatg
    16741 cttcagcaga caggtacagt ttttttctta ttgaacgttg ggctgatggt cgggctgagt
    16801 ctattgttat ttttctttct tttgaaaaaa atgagagcga atcttttttt attactgatg
    16861 tcagggatga ttttaggtac gctttttggt agtgtcagta cctttttaca agtcatcatg
    16921 gatccgaatg aatatgattt gcttcaaggc agattatttg caagctttgg caatatccag
    16981 agtcagtatc ttttgcctgc ttctttgatc attatcggct gctggtacct gttatttcga
    17041 tatcgacatg tgctagacgt tttccatctc ggaaatgaac aagcagtaaa cctcgggata
    17101 gacacagatc gttttgaatt tttgatttta tgttgtgtca gtcttttgac agggacatca
    17161 acggctcttg tgggtccagt tactttttta gggtttattg tcgcaaatat cagttatcgt
    17221 gtcttttcta cttatcgtca tggtcagcta tttatcggaa gctttctggt agctatgctg
    17281 tttctgacag gaggacagct cgcagtagag cagattttcg gctggaatac aacagtgagt
    17341 gtcgtcgttg aatttgtcgg aggaatttat tttgtcacaa aattgattcg cgaaaggaag
    17401 agtatcctat gattgattta caaaaggtca ataaactgta ccatggaaaa tcagtggtta
    17461 aagatgtaca gttaacgatt cctgaaaaga aattgactgc ttttatcggt ccaaatggtg
    17521 ctggaaagtc aacggtgttg tcgatgatca gtcgtttgat accaaaagat acaggtgaaa
    17581 tctatttaga ccataacgaa gtaaaggctt ggaaatccaa tgaattagcc aaaaaattat
    17641 cgatcctacg acaatcgaat gaaataaatg tccaaatcac cgtgaaagaa ttagtagaat
    17701 ttggacgatt tccttatacg aaaggaaaat tgacgacaaa agacgaggaa atcgttcagg
    17761 aatcactgga acaccttggg ttagaagaaa tagcggatca atcgattgac actctttcag
    17821 gcggtcaact ccaacgtgcg tatattgcaa tggtgctagc gcaagacacg gattatattt
    17881 tactggatga acctttgaac aatctagata tgaattttgc ggttcaaatc atgcagattt
    17941 taaaaaattt agtacaagaa atcgggaaaa caattgttat tgttcttcat gatatcaact
    18001 ttgctgcaag ttatgcagac gaaattgttg caatgaaaga tggacagtta tttacgcaag
    18061 gaacgaccca agaaatgata aaaaaagatg tattggatga attatacgaa atggacatac
    18121 ggatttgtga gattgaaggc cgcagattct gtctttattt ctaaaactaa cattaggaga
    18181 gaagagatga aaaaattagt agtaggtatc ttgattctag catcatttgg tttagcagca
    18241 tgtggaaata gcaacaacac cagtcaggca gacacaaaaa aaagcagcac acaaactgaa
    18301 actacattga cgataacaga tagtaatgga gaccaaatcg aggtgccaaa taatccagaa
    18361 aaagtcgtcg tattcgacaa tggttcttta gatacgatgg atgctcttgg tgttggagat
    18421 aaggtagttg gagcagctac aagcagcctt cctgagtatc tttcatccta taaaaaagta
    18481 gaatcagcag gtggaataaa agaaccagac ttagaaaaaa ttaatcagct tcagccagat
    18541 ctaatcatca tttctggccg acaaagagat tttcaaaaag atctaagtca aattgctccg
    18601 accattttcc tttctttaga tgcgaaaaat ccatgggaat ctttccagca aaatgtcact
    18661 gctttagggg agatctttgg taagcaagaa gaagctaaaa ctcaattaga ggaattatct
    18721 agtgcaatcg atcagacgaa aaagaaagca gaagctactg acaaaaaagc actagtcaca
    18781 cttgtgaatg aagggcaatt atcagcctac ggatctggtt ctagattcgg tttcatccat
    18841 gatttatttg gctttgaaca agcagatgat caaatcgaag catccactca cggtcaaagt
    18901 gtttcttatg aatatgtgct ggaaaaaaat ccggatatct tatttgtagt agacagaaca
    18961 aaagctattg gtggagacga cagcaaagat gatatttctg ccaatgaact ggtagcacaa
    19021 acgaatgctg gtaaaaatca gcagatcatc tcccttgaac cagatgtctg gtatttaagc
    19081 gggggcggtt tagaatcaat gaaactgatg atcgaagatg tgaatcaagc atttaaatag
    19141 cataaataac agaggattaa actctcatac tatgtaacaa taatgtttct tacattctta
    19201 aatagcaaga gcctatcctc aaagaagtta tcaaaaacca tccgtaaaat cgtattgatg
    19261 attttacgga tggtttttat ttatttgaga aatctttttt caacgtgaat tcattatcta
    19321 catcatacgg gttgattcta gaatggggag tggcaaaaaa tagtgtgatc cattttttca
    19381 gcatcaagat cgcctccttt gatttgaatc aaaggtacaa agcgaaactt agataaaact
    19441 taaatttaga tatttttatt ttgtaccgat ctcttcttta aacgatgttc catctgctgc
    19501 ttctcggcaa taagtccact agactagaaa atatgagaag ctattttctg tccagcgttc
    19561 ttattactca aagcaaatcg cagttgtcac aatctcttct aaacgatgtt ccatctgctg
    19621 cttctcggca ataagcccac tagactagaa aatatgagaa gctattttct gtccagcgtt
    19681 cttattactc aaagcaaatc gcagttgtca caatctcttc taaacaatgt tcaatcagct
    19741 gaccttcggt attagctcaa agcactgatt tcacaacctc tatttaggca tgtgtctcag
    19801 caagcggttg atagttgatg atctgcattt ttcgataaaa atcggcggga agcttactcc
    19861 accgttttaa taattgatag aaataactct cttctgcttt gattttatac tcgcttaata
    19921 tcaatccaaa actattcgtt ttttttgaag ctaatttttt ataaatatcc aatgctttgg
    19981 ttttatcttc attgacaaag agtgttttta cttcaatata agaaccctcg atattttgtg
    20041 tttcttcgat aaaatagatg ttttttatat attccttcat agtgattcct ccaaagaagt
    20101 cttttatttg ataagtttac tctaccgaag agttgttaat aatccgtgaa caaaaagtaa
    20161 ttctttttcc aatagttgtt aaaggttttt tattttttat agtatttttt gttatttaag
    20221 aaaggaataa ggttaaacaa ataacaggtt gctttttggg tctttttccg ttcaagaaac
    20281 tgaacgaaga aaagggaaag ttgtctcttt tgtgataaga gaaatcataa taaacgaatg
    20341 acttactttc caataaattg agaacagtca gaaccagaca aattagttgc atctgatgaa
    20401 taaagtgatt attctatgac atgaggtgat acagatgaaa ttagaagtga caaaagaagc
    20461 acaagaagta ttgaagaata aattagaagc agacgatcaa cttttattag atatcgaaaa
    20521 cggtgacggt ccatttgcag aaagccaagt cagttgccaa ttggatacgg catttcgatt
    20581 gatcattgtc aaaaaagata cacaaacaga tctaagtctt tattctgtag tggctgatac
    20641 accagttgga ccaatcaaaa tcaaagacag tgcaatactt tacatggatg atccatccac
    20701 acttgcttta gaaccaactt ataatagttt acaattaaaa ggaccaagcg gcttgagaaa
    20761 aggcaacttg caagttgttc gaaaagacta gagggataaa atgggaaaat tggcgatcat
    20821 cagcgaccta catgtggaca tcaataaatt cggcgaatca gagctgatgc agctttggca
    20881 agttttgcaa aagcacaaga tcactcggct tcatctagcc ggagatacgg ctaataaagt
    20941 agaccgttgc ctagctgttg tcgaattttt cgtcaataaa ggattatcta cgacatttca
    21001 tttcggcaat cacgagttgg cagatgtgcc caatgaacag atgatggacc attttccaga
    21061 cccacatttt ttaaatgaac ggtatattgc tttaaatgag cagaccgttc ttttagggat
    21121 gaatggctgg tacgactatc agttttccga attgaaagat tacgaggcaa tcgtacgaat
    21181 gaagcgtttg tattggtatg atcggttcat ccagcgtggg aaaacagatc tcgaggtcaa
    21241 tgcagaaatg cttgcagctg caaaaaattt gctagaccag ctggaagcaa aaaaacttga
    21301 ggtgatttta tccactcatt tcgttcctaa acgggcattt atcatgtatc aatctgctcc
    21361 ttatgagcgt tggaacaaat taaatgcatt tttaggatca gaatcattcg gagaattact
    21421 tgaccgttat tcaaatatca aacaagtagt ttttggccat acgcatcgtc gctttgaaga
    21481 taaatccatc ggtcacacga tttacagctg cagacctttt ggttattatt atgaatggca
    21541 gctgacccga gattttgtct tgaaagaaca gttgctagac agctatgatc ctatgaaatt
    21601 acgtgtcctc ttgcgtgccc atcacccagc ttttcaggcc tatcaaacac aacatttgca
    21661 agaggaattt gaacaagcga tgacaatcgt tccgtattga gacagcatta gtaatgtgta
    21721 aaagaaaata ccttgtaaaa cataaggata agtaagagca gtagatccat ttatcgtgga
    21781 tatgctgctt tttttgcgga aaataatcag cgaatgttta tttgatcgga tgatttgtaa
    21841 ctcgcagagt actagatgca agcgtgtatt ctttttttga acgataatca caaaaaaaaa
    21901 tataatatac attttttatt taattataat taatattgat tgaagtctag gcagtcaagt
    21961 cacattttga tagataggag ggggacttac gaattatttt aaaggctatg acagctgtat
    22021 ttctagattg tgtttgcatc aagttgcaat aaaaactaga aatcaaatcg ggctgataat
    22081 catttataac taatcgatac ataggaccaa atataggtct atgttctttt atttcataag
    22141 tatcgtaaaa cagaattttg ctatggatta atccaatcga ctgatatgtt tagtaaataa
    22201 agaaagagga atgtcaatga tgaaaaaaga aatcgtatta acagcagcaa tgatgttatt
    22261 cagcatggta gcaagtacga cttttgtttc tgcaactgaa gtatacccaa aggaatataa
    22321 cacagaagga acgattacat ttgaagcagg agatgagggt gtaactcctc ctgttgatcc
    22381 agagaatcct gatcccaata aacctgtcga tccatcagat cctccaagcc ctggaacagg
    22441 aggggcatta tcgattgatt atggatcaaa attcaaattt gggactcaaa aaatttctac
    22501 tgcggataaa acgtattatg cagctgctga tgtgatgaac gatgggtcta gaaaaccgac
    22561 atatgtccaa gtgacagata ggagaagtac cttatctggg tggaaattaa gtgtctctca
    22621 accagaacag tttaaaactg ccagtgggga tgaactagtt ggagcacagc tgaaatttac
    22681 gaaagggcag gctgtttctt tggttgatcc aacatatacg ccgcaaacgg tgaattctga
    22741 attaacgttg acaccgggag gaaacaatac gttagcaatt aatgctaaat caggaacagg
    22801 tgtggggact tgggtctatc gatttggtgc aaatgaaaat gaaaatcaag atgcggtaca
    22861 gctttcggta cccggtaaat ctgtcaagtt agcgcaacaa tattccacta aattagtttg
    22921 gacattagaa gatacaccta acaactagtt tactgaaaag aactgttaaa aataaatacg
    22981 tagtcatatt gtcttgcgag gtaatcgagc aaaaattgtc aaaggctagg ctaaatttgt
    23041 ttggatacga atagttcatc taggaattta acgatcagga ggaaaagata tattgaaaaa
    23101 gaggaggatc gtgctcattt taagcagtct cttagtgact aatatctttc cgatcatagg
    23161 gtactctttg gaattgaatc agttggagca tggaaataga agagacgaag aaagcaaact
    23221 cacttcgaca ttcgacagtt taatagaatc caaccagaca caagaaagca gtgaggattt
    23281 aagtatttcg agtagtggag agaccactga caacgtagaa gctgagggtt cggacaaaca
    23341 aggactagaa gatgcaccaa tttctgcggt tgagctgcct gacgatccca atattatttc
    23401 tatcgataaa atttttcaaa cacctatcgg tactagcacg tccatattgg aaaatgggaa
    23461 gttactgcag ttgaacccag cagaaaggtc tcaaagagga gctatttggt caaataagaa
    23521 gattagttta ctctcggatt ttacatttaa atcttattta tacttaggaa atcaacgaga
    23581 tagagcggga gatggcatga cctttacatt gactggcgat cctagaatgg ctagtaatcc
    23641 cagtcaagtt attggatcgc caggtatggg aattggctca tattctactc gttctggcca
    23701 accttacatt gcgaatgcgc tctcgattga gtttgatacg tataaaaata gtggttcttc
    23761 tgatcgaatg gatcgagaga taactgctga taatagaggg catggacatg tcgcttttgt
    23821 cacgcctaaa ccaaataata ataattattc tggagagcat actggcgtga cggtagctcc
    23881 tacatttctt tctaatggca catggcgctt gttaataatt aaatgggacg cggagcaaag
    23941 aagtcttact tatgatttgg aaggtgtcgg tcgctcttct cggattatac ctgatctcat
    24001 taggcagttt ggttctacag aagtaaattg ggggtttact tcttcaacag gtactttttt
    24061 tcaagaaaat gctgtcgctg tcacgcgact tccatcgaat gtcacatcaa cagctgatgt
    24121 aaaagtcaac gatagtgatt atggggccat cgctgaagct ggaggaaacg ataagataac
    24181 tatcagaaat acattgaagg tcgaggggga ttttcttgaa tataagaaaa tgcagactag
    24241 tatcgacctc ccacaagaat taactattcc cgagcatatc ttgatcaatg gaaatcaaat
    24301 tgatgccaaa gatatcttga taaccggaaa tcgagtcacg ctaaatctga cagattattt
    24361 tattgctgga gatacgttaa taatcgaatt ggagacaact ttgcaggaag taattcctga
    24421 aaaagtactc actatgcatt ttgaatacct tgaaaacggg aatttgattg agcaatcgaa
    24481 tgaaataaac ctaaagatcg caaagctgaa ggagatagat atccatgttt attataaaga
    24541 taaagacagt cagattgata ttgctgaaat gaaaacaatc actgggaaaa taggagagaa
    24601 gtataaagaa actcctattg atattgaagg gtatgttttt gactcggatt cagcgaatac
    24661 agaaggcatc ttctccgaaa atacagagga tatctatttt tattacagaa aaggcaaact
    24721 ttatctctca actgctcctc aatatgtcaa ttttggaaaa cataaaatat ccagtagacc
    24781 attaagtgaa ttcggacagt tttcaggtgg gttgaaaatt atagatgaac gggcttttgg
    24841 aagttggaaa ttacaattga aacaagagaa agttcttaca aatggagatt ttgagcttcc
    24901 agaagctctt tcctttgtta cctctgaaaa tacaacggtt atagggacat ccgctgttac
    24961 gattttcgaa agtgatcaaa agggtgaaag cgatttgtcc tctcttttag attctacagc
    25021 tcatcgtgga atcagaatag atattcctgt agaaaaccaa agaatcggaa cctttgaggg
    25081 gaaactatct tggatacttg cagatgtccc aggaaattaa caggaagaga agaatataac
    25141 aaaattattg agtttcaaga agggcacagg taccataggc tcttgtgtcc cacagagaca
    25201 agattttgtt atagtcttct cttacatatg attaaaggag cgattttctt gagaccaaaa
    25261 caacaattat ttataacact cctagttatt ttaggggcac tttttaacaa tatatcagtg
    25321 tcagctagtg catttaattt ttcagtgacg ccgataactt cagagaatca aatcgacaag
    25381 agaaaaactt acttcgatct tcaacttgat ccggatcagg aagtagaagt aaaagcggaa
    25441 ttgagaaatg atacagaaaa agaagtgaaa atcgatattt ctgtgaatag cgcaaccacc
    25501 aattcaaatg tgatggttga atacggaaaa aacgagattg aaaaagacat gagcctgatc
    25561 tttgatctgg tagattatgt gagctatcca cagatagtga cgctaaaacc taaaagtgtc
    25621 caaaccgttg cttttcatgt caggatgccg aatgagcgat ttgatggtgt attggctggt
    25681 gggatcacat tccaagaaat ccagacggaa aaggaccaaa cagaaactaa ggatcaaagc
    25741 ttatcgattg agaatgaata cacctatatt attgctttgt tgatgcaaca gacactgaac
    25801 gaagttgctc cgaaccttac tcttcatgaa gttaaaccag atcaaatcaa tgcaagaaat
    25861 gtcatcttag caaacgttca aaatgatcaa aaaacctata tcaatcaagt agttattgaa
    25921 acaaaaatta caaaaaaagg acattctgaa gttttatatc aagaggaaaa agaaggattg
    25981 cagattgcgc cgaatacaaa tttttctttt ccaactgctt taaatggtca accgctaacc
    26041 ccaggtgaat atcaccttac aatgactgtt ttaggaaatg aaaatgaaaa tggaaaattt
    26101 tcgagaaaaa aaggaaatac tacgatcaac tataccaatc aatgggtatt tgaaaaagat
    26161 tttacaatag acggaaaagt agccaaggaa ttgaacacga cggatgttac gattaaaaaa
    26221 gacaataatt ggatttatat cttagtagct tcacttttct tattgtttat tttattatgc
    26281 atacttttgt ttgttttgag aaaaaaagaa gagaaagaaa agtaaggtta agataacaaa
    26341 tagtaaattt ttattattgt aggatggttg ggctcttttt ataacaatat aaaatatacc
    26401 attttattaa taaaaatgca tactagtaaa tgatataatt ctaataacag gtttgatcgc
    26461 gagaaaggcc aaataaaacg ctctccaaaa tgttaaaaaa aagacaggac gatgtatatt
    26521 atacaaacaa gaatcgctga caacagtgac tcggatccag gaaatcagtt ttatccccag
    26581 ctagttggtg aatgcatgga aagtaatata cgtagaaaga gctgatgact aatgaattat
    26641 gagttttttt taagcaaaga agatcacaga agttattcat tacttaaata tttagaagca
    26701 agctcaaatt tatcagagtc aatatcgaat gttcaagaag agttgtcgct atcgacattt
    26761 ctcttgaaaa aaacgatcga taagctgaaa catgatttac aaaagttcga tctagagaaa
    26821 aatttgcgag taaccgtcag tgaattggat atatgtctgg aaattaatgg aaaatgttct
    26881 agcaaagctc tgttatctcg ttatataacg gattctttat ctatgaaaat ggtattggct
    26941 ttctttcagg aaaaatatat ctcagtagaa aattttgcta aacagcatca cgtgagttat
    27001 tctgtggctt ataaagtatt acaaagacta aaaagaaatc taaaaaaata tcaaattttt
    27061 tttgaaaaga gaaagttg
//
LOCUS       DAFEOM010000031        25694 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.032, whole genome shotgun sequence.
ACCESSION   DAFEOM010000031
VERSION     DAFEOM010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.032"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            126..3653
                     /locus_tag="LP524_001510"
     CDS             126..3653
                     /locus_tag="LP524_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBL2234569.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3650..7372
                     /gene="addA"
                     /locus_tag="LP524_001511"
     CDS             3650..7372
                     /gene="addA"
                     /locus_tag="LP524_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBL2234570.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7441..7782
                     /locus_tag="LP524_001512"
     CDS             7441..7782
                     /locus_tag="LP524_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2234571.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8091..9134
                     /gene="pheS"
                     /locus_tag="LP524_001513"
     CDS             8091..9134
                     /gene="pheS"
                     /locus_tag="LP524_001513"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBL2234572.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9139..11559
                     /locus_tag="LP524_001514"
     CDS             9139..11559
                     /locus_tag="LP524_001514"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBL2234573.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11729..12739)
                     /gene="trpS"
                     /locus_tag="LP524_001515"
     CDS             complement(11729..12739)
                     /gene="trpS"
                     /locus_tag="LP524_001515"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBL2234574.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12993..13730
                     /locus_tag="LP524_001516"
     CDS             12993..13730
                     /locus_tag="LP524_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBL2234575.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13744..14559
                     /locus_tag="LP524_001517"
     CDS             13744..14559
                     /locus_tag="LP524_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBL2234576.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14572..15219
                     /locus_tag="LP524_001518"
     CDS             14572..15219
                     /locus_tag="LP524_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBL2234577.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15235..15891
                     /locus_tag="LP524_001519"
     CDS             15235..15891
                     /locus_tag="LP524_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBL2234578.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16053..16874
                     /gene="racE"
                     /locus_tag="LP524_001520"
     CDS             16053..16874
                     /gene="racE"
                     /locus_tag="LP524_001520"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBL2234579.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16877..18232
                     /gene="rph"
                     /locus_tag="LP524_001521"
     CDS             16877..18232
                     /gene="rph"
                     /locus_tag="LP524_001521"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBL2234580.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18233..18751
                     /locus_tag="LP524_001522"
     CDS             18233..18751
                     /locus_tag="LP524_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBL2234581.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18858..19358
                     /locus_tag="LP524_001523"
     CDS             18858..19358
                     /locus_tag="LP524_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBL2234582.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19638..20558)
                     /locus_tag="LP524_001524"
     CDS             complement(19638..20558)
                     /locus_tag="LP524_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HBL2234583.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20723..21736
                     /locus_tag="LP524_001525"
     CDS             20723..21736
                     /locus_tag="LP524_001525"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HBL2234584.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21846..22601)
                     /gene="fabI"
                     /locus_tag="LP524_001526"
     CDS             complement(21846..22601)
                     /gene="fabI"
                     /locus_tag="LP524_001526"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBL2234585.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22821..23261
                     /gene="fabZ"
                     /locus_tag="LP524_001527"
     CDS             22821..23261
                     /gene="fabZ"
                     /locus_tag="LP524_001527"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBL2234586.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23376..24137)
                     /locus_tag="LP524_001528"
     CDS             complement(23376..24137)
                     /locus_tag="LP524_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL2234587.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24326..25420
                     /locus_tag="LP524_001529"
     CDS             24326..25420
                     /locus_tag="LP524_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBL2234588.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(25506..>25694)
                     /locus_tag="LP524_001530"
     CDS             complement(25506..>25694)
                     /locus_tag="LP524_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL2234589.1"
                     /translation="NSNGILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
BASE COUNT         8509 a         4454 c         5416 g         7315 t
ORIGIN      
        1 gtatacttaa aaaagtagaa atatcagaac cttaaaagga tataatttga agaatataga
       61 acaaatgttc agtatttttt gatataataa aggagcaccg tatcatgtgt tgaaggagga
      121 aaagcatgtc tttacaattt attcttggaa acgggaaaaa agatcaccgt caagtcttga
      181 tcgacgaagc atccgcttgg ctcgaccagg atgcaagtaa ccaagtattt ttccttgttc
      241 ccaactacag caaattcgaa caggaacaag aaattttagc ggagatgcga agaagaagcg
      301 gaaaaaaaac tttcagcaca acaaacgttc aagtatttag tttttatcgt ctggcttggt
      361 atttgctgca acagacaact cttcttacag gaaatgaatt atcagaatca ggatctgcaa
      421 tgatcttgcg acagatttta gaaaaaaatg aggaagaatt aacaatcttt cgcggagaaa
      481 tcaataaaag agggtttatt cgtcaattac aagaactcta cagtgaattg cgtacaggaa
      541 atattcaacc agaagacctg atgcttttat ttggtgattc acctaatgca gaagaccagc
      601 aacttaaaat gaaagacttg aagctgattt tttcagctta tgacagcgaa ttgactcaac
      661 gtgcactaac aaatgaagat gcactatcta tactagcaaa ttatatgcag acacaagatt
      721 tcaacaacgt caaatttatt gtcagtggtt tttctcgaat caatggtcaa gagtatcaat
      781 tactgcaggc aatgatggaa aaggggcaat tggttgtcga tttattgctg gatcgtcctt
      841 atacagcgga tcttccagaa gtacttacgc tctttcatga gactggaaag ctttatttcc
      901 gtctttttca aagtgcaaga gaacggaaag tccctatatt agtggaccga tttgcaccag
      961 agcagactcg tccgacagag cttcaactgc tgcaaacaat gtgggaagag acatctagcc
     1021 aaaagaaagc ggcagtacac aaaatagggg gacaagagca tctccatttg tgggaagctg
     1081 aaaatccaac tgaagaagtc cgacaattag ctgttgaaat cagacgacta gtcagtgaag
     1141 aaaactaccg ttatcgtgat atccaagtgc tgacaaaaaa tatgtcttta tatggaaata
     1201 tccttcggcc gatctttcaa gaaatggcaa ttccttttta tatcgatgaa gaacaaatga
     1261 tggaaaatca tccgctgatc gaatggatca acagtctgtt cgctcttgac cgctattctt
     1321 atcggctaag tgatattatg cgttttttca aaactgaatt atttctgtcg acggaagcag
     1381 aaaccgaact tgatgaatgg aaacaagagc gagatgaatt caggaaaaaa ctggacatca
     1441 ctgaaaatgt agcacttgct tacaattatc aaggaactta ttggactcgt gaaaaagact
     1501 ggcagtttgt ggcgtatgat tttgaagcag aacagattga agacgtacag tatttggaag
     1561 aacagtcaaa tacgattaga cgattatttc agaaagctgt accagtattt ttgaaaaaaa
     1621 taaagaaaag caaaacagga caagaggccg caaatgtttt ttatcaattt ttgatccaaa
     1681 gtggcgtaca gcaacaatta ttgttttggc gtaatcagga agtagacaga ggaaacttag
     1741 aagcggcaag aaaccatgag cagacatggg gagcgctgat tgatctgttg gatgaatatg
     1801 tagtaattta tggtgaatcg gcttttgact ggtcactgtt ccaagaaatc attacaagtg
     1861 gtctagaaaa tctaagttat ggtaaaatcc ctaccgcaat cgaccaagtg cgaatcaatc
     1921 gtttggaatt agttcgacca aatcaagctg ccgtcacttt tgcattaggg ttgaatgatc
     1981 aagtgtttcc agaccgaaaa gagacaaaag gattgttgtc cagtgaagaa cgagaagtat
     2041 taaatcaggc cttgccagat ggacagtttt tattcgaccc atcaaaggaa agtatctctt
     2101 ttgagccttt ccaagcctat cttgtttttc tttccggaac agatcgatta tatcttagtt
     2161 atgcacaaag ctatgacaca gatggttcgc tgaagatgtc tccttatttg cgtagaatca
     2221 cagaatattt atctgttccc gtcgagagaa aagagcattt gacgatcgaa agcgacccta
     2281 attgttatgt agggacttac agaagcggaa tcaatacagt caataggatt tatcgattag
     2341 ctttagatga aaaaaaacct gtaccaaagc actggcaaaa gctcaaagaa ttagtactga
     2401 attcgtcatg gcgaaatttt gctttacaga tttttgaaag tcaggaacat ttaaatgttc
     2461 ctgtttcgat gccagaaaaa atggcggaag agctttatgg aaaagagatt tatacttcaa
     2521 tttcacgtat ggagaatttt tataattgtg aatacaaata cttcatgcaa tttggtttga
     2581 aattgaaaga acgaactgtt tatggtctaa ctccagcagc aactggtgat ttttatcatg
     2641 aatcattgga tcgtttcttc aagctactgt tttcaaacca attatcctta gtgaatatgt
     2701 ccgatgcgca gcgtagagac tttactgaaa aagtgctgca ggaagtattc ggagaacttc
     2761 ggtttgagat attggatagt tctgcaagaa tgaactatat ccgctaccag ttaggacaga
     2821 cgattcaaaa agtgacatgg gctttgcaaa aacagagcaa aaatacaggc atgcatcctc
     2881 ttcagacaga gattctattt ggtcagattg ctggagcaaa aggtatcaca ggtctggaat
     2941 taccgttgag taatggtgga aaactccatg ttcgagggaa aatcgatcga atcgatgtag
     3001 cttctgaaca agaagatact tggctaagcg tcgttgatta caaatctagc ggacgttcat
     3061 tcgatgtcac agaagcttat tatggcatgg ctatgcaatt attgacgtat ctggacgtag
     3121 cattgatgga tgcggtacgt ttgactggaa aggcggctgt taaaccagca ggatcatatt
     3181 atttacatgt tcataatcct gtattgacgg agacagatga tgtagagaag aatacattga
     3241 aaaaatttag ttatgatggc gtttttgtcg atgatcccgc tcttttagaa gtactagatc
     3301 attcactgga agcaaaagaa caatcactga tctatccagt gaaaaaaaat gcgaaagatg
     3361 aataccaaaa aggtagtttc agtaaagaca aatttttcac ggaagaagaa ctgcaactat
     3421 tcatggcgca taatcgggaa aatatgcggt tagctggaga tgcgattact tccggagaaa
     3481 tcaaactaaa tccagcttat aaagataaac agcggattgc ctgcgaattt tgtccattca
     3541 gaagcatctg tacgtttgat gtgatgctga aagaaaataa ttaccaccgt ctggagaaaa
     3601 tagataagga agaagcactg cgtagaatat tgaaaaggag tgaagaagag tgaatcaaat
     3661 tcctttaaaa ccgcaaaatg aacgattcac agatgatcag tggcaagcga tttttgatca
     3721 aggagataat ctattagtct ctgcgtctgc cggatcaggt aaaactacag tgcttgttcg
     3781 aagagtgatt gaaaaactaa aaatgggttt tgatatcgat gaattattga ttgtgacatt
     3841 tactgaagct gccgcaagag aaatgaaaga aagaattcaa gaggcattac aagaatccgt
     3901 caatagcgaa agtgaccctg tacgtagaca gcattttact aagcagttag ttttattgcc
     3961 cacagcgaat atctcaacct tacatgcttt ttgcctgaca gttattcgac gttattatta
     4021 cttaattgat attgatccag tctttcgcat gttaacagat gaaacagaaa cgatcttgat
     4081 gaaggaggat gtgtgggacg agctgagaga agcactttat gcagaaaatg atgaacgatt
     4141 tttccaatta acaatgaatt tttctaatga tcgttcagat gacggcttga cgaatctggt
     4201 gttttctctt tatgagtttg cccgtgccaa ccctgacccc caaaaatggc tagaacagtt
     4261 aagcgataat tatcgtttgc ctgaggggct agcgaaatct cgattatatc aaaaacaaat
     4321 ccgtccttta gttttggcag atatatacca atgtgtacag ttatatgaac agatgactca
     4381 gttagcacaa ggtgaaggat tagaaaaaat gcatgagcaa gtagctggag aacaacagca
     4441 gatcaagaat atctatgaag cgttctcgca agatcggtta gaagaagctt atgctggttt
     4501 ggaacaacta actttttcaa cttttaagag cagtcggaaa gcagaattga aagaaatttc
     4561 caatgaagtc aagggcatga gggataaagc aaaaaaactg attcagcaga taagtaaaag
     4621 ttattttcct gtttccccaa gccaaatgga agaattaacg gacaaagcac taccgttagt
     4681 cgaagaaatg acaaaagtca cgcaaagctt catggacggt ttcagtatgc gtaaacggga
     4741 aaaaggtgtg ctggatttca atgatctgga acatttagcg ttgcagattc tcacagaaaa
     4801 aacgaaagat gtatggctgc caagtgaagc ttcaaaacat tatcgaaaaa aattcaaaga
     4861 agtaatggta gatgaatatc aagacgtgaa ccaattacaa gaagccattt tatactggct
     4921 aagagaaccc gatgatacaa aaggaaatat gttcatggtt ggggatgtca aacaatccat
     4981 ctattctttc cgtttagcag atcctagttt atttatcggg aaatacgaga atttctcaaa
     5041 gaaagaaggc ggacgtcgaa tcgttttggc tgagaacttc cgctctagaa aagaagtcct
     5101 ttcttttacg aatcttattt ttgaacaact aatggatcca gcagtgggtc aaatcaatta
     5161 tgacgaagca gccaaactga tccaaggatt ttcagacttt ccagagaatg aacagtttga
     5221 gccggaaatc atgatctatg aaaaagaaca ggaagaatca gagattgaga ttccgacgga
     5281 tgatatactg gaagacaaaa cagaaggcga actttttatg acaggactaa aaattcgtca
     5341 attaattgat tcctccttta tgatttatga taaaaaatca aaaaaaagtc gtccgataga
     5401 gtacaaagac attgttctct tgacacctac aaagaagaac aacttaacga ttttagagat
     5461 tttcaaaaca ttggatattc cattagagat gaatgatgct caaaactatt ttcaagcgac
     5521 agaaattcgc acgatgatct cgctgctaca attaatcgat aatccctatc aagatattcc
     5581 gcttgcggct gtattgcgct caccgatcgt gggtctgatt gaaccagaat tagccagcat
     5641 tcgattagct aaccgtgcac atacttacta tgatgctgtg cttgcttatc aagcatcaaa
     5701 cgaagatgaa ttagcagcta agctggaaca ttttggcaag caactagagc attggcgaga
     5761 acttgctcgc cgttcttcga tcactgactt gttatgggat atctattatg agacgggata
     5821 tctagagtat gtggtcggtc tgccagcagg ggcacagaga caagccaatc tttatgcatt
     5881 ggttgatcgt gccaaagctt acgagcaaag ttctttcaga ggattatacc agttcgtgcg
     5941 tttcattgaa aaaatgcaag aaaaagataa agacttagca gaaccagtca tttcaataga
     6001 agataatgct gttcgcgtta tgacgatcca tgcaagtaaa ggcttggaat tcccagttgt
     6061 atttttgtta gatatgacaa aggagttcaa cttacaagac ttgcgcaatc ggtatgcttt
     6121 tgaagaaaaa cttggagcag gaattcgcta tatggatcct gaaacacgag tgttatatga
     6181 tacgttaccg tttcaagcca tcaaattggc aaagcaaaac aaattgttgt cggaagaaat
     6241 gcgtaaattg tatgtaggat tgactcgggc agaacaaaag cttttcattg tcggttccta
     6301 taaaaataaa gagcaaatga tccaaacgtg gtctgaagca gctgatcatg aggagttagt
     6361 ttttgatcct gcgctacgtt tgaagggcag aagcagcttg atgaattgga ttggttacgg
     6421 attgatccgt catcctgaaa tgcagaaata cttagaggaa gaaatttcga cttctcttct
     6481 tcaacattca aatgcacagt tttctatttc atggatgaat cagcaatcga tcattgaaca
     6541 aagacagttg cttgctgaaa aagaactggt taacttagat cagcagatga aagaagatga
     6601 aactttatta gcagattcgt tacaaaaacg tctggcatat gaatatcctt atcaagcttc
     6661 ttcacaaacc acaagttatc aatctgtatc tgaaatcaaa cgattattcg aagatccaga
     6721 tgatacacaa gaatcacgtt tgactctgga aagcagtcag aacaaagcag catcccgtca
     6781 atttcgttat acgcaagaac aactagcaga accaaaattt ttgcaaaaag atcggcaagt
     6841 gtcagcagct gctgttggaa ctgctacgca tgctttgtta caattactgc cattagagat
     6901 gccaactaca gagagtatcc atcaaaaatt acaggaattg gtaaggaaac ggttagtaga
     6961 cgaaaaagtc gcgaaaaaag tagatgtttc ttctattata tggttctttc aaacagaact
     7021 tggtcaacag ctgattgcca acaaagaaaa cgtaaaacga gaacaacctt tttccatgct
     7081 gcttcctgct gacgaagtat ttcaagacta tccaaatcaa gaagatgaac tattaatcca
     7141 cggaatcgtc gatggatacc tagaagaaaa agatcatctc aatatctatg attttaaaac
     7201 agattttatt ttacatccgg atgatccagc agaaatagat gcaatcgtcc aaaaatatca
     7261 aggacagcta agactttacc aacaagcaat gtcagaagct ttgaataagc cagtagaaaa
     7321 tgttttccta attttgcttc gagtgaaaca aattataaac attaacaaat aattataatt
     7381 gagcaaactt gacttatgtt ttgtaaggat tatgctatac tctgggaaga ggtgagtatg
     7441 atggagctaa cacagacaga attcgtttta tgccctaaat ttgaaaaaac attctctatt
     7501 ttaggaaaaa aatggaacgg attgattatc gatgtactac tcgaaaacgg tccgcaacgc
     7561 tttgttgagc ttgcggcaaa aatcccgatg gtcagtgatc gagtattagt cgaacgcttg
     7621 aaggaattag aaaaagaagg aatagtcact cgaaactttg cctgtagcga aaagagccgt
     7681 tcagaatatc tcttgactga aaaaggagca gacttgcaaa aagcgatgcg tcaaatacaa
     7741 gtttggagcg aaaaatggat cacagaggaa gaatgcggtt gattttgtaa aaaaaatctt
     7801 gtaaactata gtccatacga ataagtaaag aacgatgacg aaaaagagta gtttggctaa
     7861 accagttcag ggagatctgt cgcagactga aagcagatcc tgtgtttgaa aaatgaagtt
     7921 cactttccga gttcgtctga taaaatcaga ccggcaattg tcgttaacga agtaagagtg
     7981 cggtgtaagt catttatccg caaattagga tggtaacgcg aagacctcgt tccttaacag
     8041 gaatgaggtc tttttgtttt gtatttggaa tttaaaggag gaacaagcac atgtctctac
     8101 aagcacaatt agagagcttg aaagaagcaa cgctcgtcaa aatcacagcg gcacaagatt
     8161 taaatgaatt aaatcaaata cgtgttgaaa cgttaggaaa aaaaggaccg atcacagaag
     8221 tattacgtgg gatgaaagat ctaagtccag aagagcgacc aaaagtcgga agtttcgcaa
     8281 atgagatccg agatcttttg acagaagaga tcgagcgaaa aaaacaagta ttagaagaag
     8341 aagcgttaaa tgcagctttg gcaaaagaaa caatagacgt gacactacct ggtagaaaag
     8401 taaaacatgg tacacgtcat atcgtgacac aaatcatgga agaaattgaa gatatttttg
     8461 tgggtatggg ctatcagatc gtagaaggat atgaagtaga atctgatcat tacaatttcg
     8521 aacggatgaa tctgccaaaa gatcatccag caagggacat gcaagatact ttctatattt
     8581 cagacgagat attgattcgg acacatactt caccagtcca agcacggaca atggaaaaac
     8641 atgatttctc taagggtgct ctacgaatga tctcgccagg gaaagtcttc cgcagagata
     8701 cagatgatgc gacccacagt catcagttcc atcaaattga agggctagtt gttgataaaa
     8761 acatcacaat gggcgacctt aaaggaacat tagaagtcgt aatgaaaaaa atgtttgggg
     8821 aagatcgtga aattcgtttg cgtccaagtt atttcccatt tacggaacca tcagtagaag
     8881 tagatgtcag ctgtttcaaa tgtggtggtg ccggttgtaa cgtatgtaaa tacactggat
     8941 ggatcgaaat tttaggagct ggcatggtac acccgaatgt gttgaagatg tcaggaatcg
     9001 atccagaaga atattcaggc tttgctttcg gccttggacc agaccgcgta gctatgttgc
     9061 ggtatggagt gaatgatatc cgtaatttct atcaaaatga cttacgtttc ttaagtcagt
     9121 tcaaggggga gtaagaaaat gttagtttca tataaatggc tcaatcgtta tgtagattta
     9181 tcgaatgtaa caccaaaaga attagcggat aagatgtctg tgacaggaat cgaagtagag
     9241 ggagtaacgg ttccagaaga aggcttgaaa aaaatcgttg tcggtgaagt caaggaatgc
     9301 gtaccacatc cagattctga tcacctttct atttgtcaag tcgatattgg cgaagacgaa
     9361 ctgtctcaaa tcgtttgtgg agcgcctaat gtgaaggttg gaatcaaagt gattgttgca
     9421 cttcctaact cacgtatcgc tggtaatgta aaaatcaaaa aaggaaaaat gcgtggccaa
     9481 gtatcaaatg gcatgatttg ctcattacaa gaaattggct attcagatag tgttataccg
     9541 aaagaatacg ctgaaggtat ctattatatg ccacaagaag ctgtaaatgg cgatccagtt
     9601 tttccttatt tggatatgga tgattcgatt attgaattat ctatcacacc gaatcgtgcg
     9661 gatgcattgt caatgcgtgg cgtagcttat gaagttgggg caatttatcg tcagactcct
     9721 acattcgatg acgaaaagct ggtagaatcc tcacgtcctg ctagcgataa aattcaagtg
     9781 gaagttgaag ataaagaagc agttcctgct tatcaaatca gaatcattga aggagtaaga
     9841 atccagccaa gtcctcaatg gctgcaaaat cttttgatga acgaaggcat tcgtccaatc
     9901 aacaatgtag tagatgtgac gaactatatt ttgcttttat tcggacaacc actacatgca
     9961 tttgactacg acaaattgaa atcaaaagag atctttgtcc gccgtgcaaa agaaaatgaa
    10021 acattagtaa cactagatgg agaatctcgg gaattgacaa ctgacaatct cgtcattact
    10081 aacggcaaag aaccagttgc tttagctggt gtcatgggag ggttagactc agagatcacg
    10141 aatgaaacaa caacagttgc actagaatct gctttatttg atccaatttc gattcgtcgt
    10201 acagcaaaac aatttaattt gcgtagtgaa tcctctgcac gttttgaaaa aggaatcaac
    10261 catgcgactg ttggagaagc cagtgaggtt gctgcagcaa tgatcgtttc tcttgcgggt
    10321 ggggaagttc tacaaggcgc ggtcaaaggc tcagagatca aagcagaaga cgtatcagtg
    10381 aagatttcac ttgaacgcat caatcaatat ttaggaacat ctcttgtcgt taaagaagta
    10441 aatgagattt ttgaagcact tggatttagc tatgaagaaa aggagtatgt ttattccgta
    10501 gtagtaccac caagacgctg ggatatccaa atcgaagcag atattatcga agaagttgct
    10561 cgtatttatg gatacgacca gttgccatct acacttccaa gtggagaaac ggttgctgga
    10621 agcttgtcaa aagaacaaga aactactcgt aaaattcgta caatctttga aggaagcgga
    10681 ttgagcgaag caatcagtta tgcattgaca acagaagaaa aatcaagaca gtttgttata
    10741 aatgaaagta atattacacg tttggactgg ccaatgagcg aagaacgttc tgtattacga
    10801 atgaatttga tcagcggact attagatgat atcagttata acactgcccg aaaaaataca
    10861 gagattgctt tatatgaaat tggtcgggtg ttcttccaag aaaatgatcc acgtacacat
    10921 ctgccgcaag aagtaaaaca tgcagcaatc gctatttctg gtatttggga agaaaaagat
    10981 tggcagacga aagctcaaaa tgtagacttt ttcaccatca agggtctttt agaaaactta
    11041 tttgaacaac taggcatcac tgaagatgtc cattatcaaa cagtgacaga aatgaaggaa
    11101 atgcatcctg gaagaactgc tgcgatttac cttggcgaaa aatttattgg ttttgtcggt
    11161 caagtgcatc caacgattac aaaaagttat gatattccag aaacatatgt tgctgagttt
    11221 gatttatctg ctatagtgga agctgctcaa aaagggatcg ttttcgaagc agtgacaaaa
    11281 ttcccagcag tcagccggga catcgctttg cttgtaaaag aaactgtgac aaatcaggaa
    11341 ttaacagaag ttatccgttc tgctgccgga agatttttaa cagatatcca attatttgat
    11401 gtctatcaag gagaaaacat tgaaaaagga cataaatcca tggcgtatag cctgacattc
    11461 gtcaacccag aagcaacatt gacagatgaa gaaatcaata aagcaatgga aaaagtcgaa
    11521 aaagcgttgg tagaaaacct agaggcaagt attcgctaag aaggaaaata tagtaagtaa
    11581 cgatcaagta gcttttgaag accatacatt tgaaaaaaag atgtgacaca acttcgtcac
    11641 atctcttttt ttgattttaa aaaaggaata gcacagagac gagataaaaa aacaggcctt
    11701 tacagcctgc ttttttagag taaatgaatt aatggaagta attgattccc attgctgatt
    11761 ttacttcaga aagtgtttgt gcagctactt tagcagcttc ttcactacct ttacgtaaga
    11821 tttccataac agctgaagga tctttttcca attcttctcg gcgcgcacgg atcggtgcaa
    11881 attcagcttc caatacatca atgaggtatc gtttgatctt tacatcacca agtccgccgc
    11941 ggcggtaatg agctttcatt tcttcaattg cttctttatc tgttccgaat acatccaagt
    12001 aggtgaagac catattccct tctacttgtc caggatcttg tacatggatg tggtttggat
    12061 cagtatacat actcatgact tttttctgta agacatcggc aggatcggag atataaatac
    12121 cgtttcctaa cgatttactc attttgccat ttccatcgat tccaggtaat ctacccattc
    12181 cttttggtgg gaatacgcct tttggttcca ccaagacttc accatatgta tgattgaagc
    12241 tttgtacgat ttcctgtgtc tgctctagca ttggtttttg atcctcacca acaggtacaa
    12301 gattcgcttt gaatgcagtg atatctgctg cttgagaaac aggatagata aagaatcctg
    12361 taggcacgct ttcaccgaat tttttttgtt cgatttctgt tttgacagtc ggattgcgcc
    12421 gaacgcgtcc tacgctgact aagttcaaat aatacatggt taattcagct aattccggaa
    12481 tttgtgattg gataaataaa gtagattttg caggatctaa tcccacggct aaatagtcta
    12541 aagcaacttg caagacattt gatgaaacct tctcaggatt tttagcatta tctgtcagtg
    12601 cttgcatgtc agcaatcatg acaaacagtt ggttgtcttc atctgcctgc atagccactc
    12661 gggtttttag agaaccgacg taatgcccta aatgcagctt cccagttggt cgatctcccg
    12721 ttaaaataat atttttcaaa aaagacacct ctttacgatt tgtaagtctg agcacttaat
    12781 atcttaccgt ttttcctgaa aagaaacaac agttcctaag gaaaaaacaa ggaaagagga
    12841 taaaataact aatagattag aaatggaaga aatatttatg ggagaataaa cgttctaaga
    12901 aactttacaa attactaaac tatcagatat aatgtatttt attatacatc tttaaaagaa
    12961 atcttactaa ataataatgg gagtgtaatt ctatggcaat gattaaattt gatcacgtgg
    13021 ataaatatta tggcaagttc cacgcattaa aaaatatcaa tctcgaattc gagaaaggag
    13081 aagttgtcgt tgtcattggt ccttctggtt caggaaagag cacgatgctt cgctgtatca
    13141 atggattaga gacgatctct tcaggtaagc tgctgattaa tgatactgac ttgcatgata
    13201 aaaagacaaa actgacagaa gtccgaaaaa acatcggcat ggttttccaa catttcaatt
    13261 tgtacccaaa taaaactgtt ctggaaaaca tcaaattagc accaatcaaa gtgttgaagc
    13321 aagaccaggc aactgcagtc aaaaacgcgg agaaattttt gaaaactgtc aatatgttag
    13381 ataagaaaga ttcttatcct tccatgttat ccgggggaca acagcaacgg gtggcaatcg
    13441 ccagaggttt agcgatgaat cctgagatgc tgctgtttga tgaacctact tctgctttgg
    13501 atccggaaat gattggtgat gtattagacg ttatgaaaaa attggctcat gacggtatgt
    13561 caatgattgt cgtgacacat gaaatgggct ttgccaaaga agtggcggat cgtgttatct
    13621 tcatggctga tggagaagta ttggaagaca gcagaaatgt gagagacttt ttcgaaaatc
    13681 caaatgaagt ccgagcacag caatttatca gtaaagttat caatcattga taggaggaaa
    13741 aagatgcgca ggttaaaaaa aatacgcctg attttccttt taggcattgc ttttcttgca
    13801 ctagccggat gtaaaggaaa tgggttggca aatgaagata ttttagagcg aagcaaatct
    13861 accaatgaaa tcatctgggg tgtcaagtat gatactcgat tatttggaat gatggatatt
    13921 gagagcagaa ctgtacaagg atttgatgta gatattgcaa aagcaatcac taaaaagatc
    13981 cttggtgaca atggaaaaac agaatttgtt gaagtcactt ctaaaactcg gattccatta
    14041 ttgaaaaacg gaaatatcga tgcaattatt gcaacgatga cgatcacaga tgaacgaaaa
    14101 aaacaggttg acttttcaga tgtctatttc gatgctggcc aagctttgtt ggtaaagaaa
    14161 ggaagccaaa tcaaaagtgt agatgatttg aatgcttcta caacggttct agcagtcaaa
    14221 ggttctacat cggctgcgaa tatccgacag catgcaccag atgcaaaaat tttagaatta
    14281 gaaaactatg cagaagcatt tactgcttta caatctggtc agggagacgc gatgactacg
    14341 gataatgcga ttttgttagg gattgcagat gaaaatccag agtatgagtt agttggtgga
    14401 acgtttacta atgagcctta cgggattgcg atcaacaaag ggcaagaaaa cttcttgaaa
    14461 gctgttaacc aagcgctaga ggaaatgcat gcagatggca catacgataa aatctatcaa
    14521 aaatggtttc caaatgaaac tgaaggaaaa gtagagtaag ggggagaggc aatgagcgaa
    14581 ttgatacaaa cttatggacc aatctttgtc gacgggttca agatcacatt gttttcgagt
    14641 gtcttagcac ttgtgtttag tttgattatc ggaacgttaa tggcaatctt ccaattatca
    14701 aagaataaag tagtgagcgc cttagcaaag gcatatgtag aattttttag aaacattcca
    14761 ttattaatca tcgtgatgtt tttttatgtt gtcgtaccga tttattggtt taatctagat
    14821 ggattccaag caggtacgat tggattaacg atctatacat ctgcttttat tgctgaaaca
    14881 gtacgttcag ggattcaaac cgtaccaaaa ggtcaaacag aagcaggact atcttctggt
    14941 tttacctatt cagaaatcat gcgttatatt gttttaccgc aagcattcaa aatcgttatc
    15001 ccgccattag gcaaccaatt tatcaacctc gtaaaaaact catctatttt agcaatcgta
    15061 gctggtttag atttgatgta tcaaggagac ttgatcgcta gtgaaacatt caataccttc
    15121 gatacgtata tcatcgttgg gttattttac ttagttatca cgctaccgtt atcttactta
    15181 atggtttatc ttgagaaaaa atgggcggtt cgttcataga aggaggggaa atacatggat
    15241 tttagcggcg ctttttcatg gatgaatatc cgttttttat tggaaggatt aaaggttact
    15301 gttgaagttt cattgttttc gatcattttt agtttcttga tcggtggtct gacaggtgtg
    15361 attcgttttg cgaatatccc tttcttttcc aaaatccttg gtgtagtgat tgatattata
    15421 cggaatcttc cattattatt gatcattttc ttcacttact ttgccttacc tcaaatcggc
    15481 attcagatga acatcttctg gtccgctgtc gcagcattaa cgatttttga gtcagctatg
    15541 ttgtcggaaa ttttccgagc tggactcaat gctgttccaa aaggacagat ggaagcagga
    15601 ttatcaacgg gactgactta tgtagaatcg atgcgaacga tcgtgttacc gcaagcattt
    15661 aaatcaatga tcccggcaat tgtgagccaa ttgatttcac tgatcaaaga tacttcatta
    15721 gcagtaatca tttctttacc tgaactttct catcaggcac gtattattta tggacagaac
    15781 accaattatg tgcttccgat gttcatcatc atgacactta tgtacttcgt ggtatgttac
    15841 gcgctttctc tgctttccgg ttacttggaa aagagacggt acagttatta atagatgatt
    15901 cttttagcgg ttgtggcaaa ctaaagtcac aaccgctttt cctctttcca ttcgaaaaaa
    15961 agagatttcg attgttttaa gcaagtaaga gcacttgaaa attcacgcta aaaagtacac
    16021 aatagggagg atagaattta ttgaaaaaaa ggatgatacg attgacagat aatcgcccta
    16081 tcggatttat tgattcaggt gtcggcggct tgactgtagt aaaagaagcc ctgaaacaat
    16141 taccgaatga aaatatttta tttgtaggag acacagcacg ctgcccatat ggccctagac
    16201 ccgcggaaca ggtaatccag tatacttggg aaatgacgga ttatctggtg gagcaaggaa
    16261 tcaagatgct ggtgatcgcc tgcaataccg caactgcggt ggctttagaa gaaatcaaag
    16321 ctgctctttc tattccagtc atcggtgtga tccttcccgg tactagagcg gcagtaaaaa
    16381 aaacacaaaa taaacaagtt ggcattatcg gtacgattgg tacggtaaaa agtcaagctt
    16441 atgaaaaagc actgaaagag aaagtaccag aattgactgt gacaagtctt gcttgtccca
    16501 aatttgtttc agttgtcgaa agtaatgaat accattcatc ggtggcgaaa aaaattgtgg
    16561 cagaaacatt agctccttta accactaaaa aaatcgatac attgattttg ggatgcaccc
    16621 attatccatt attacgcccc atcattcaaa atgtaatggg agaaaatgtt caactgatcg
    16681 attctggagc agaaacagta ggtgaagtat ctatgctgtt agattatttc aatttgagca
    16741 attcaccgca aaatggtcgg acattatgcc agttttatac aactggctct gccaaacttt
    16801 tcgaggaaat agctgaagac tggcttggaa tcggacactt aaatgtagaa catatcgaat
    16861 tgggaggaaa ataaggatgc gtcatgacgg gagaaaagcc caagagcttc gcaaactgga
    16921 gcttcaaaca aatgttttca aatatccaga aggatcggta gtgatccgtt ttggtgatac
    16981 aacagttatt tgttctgcaa caatagaaaa ttctgttccc ccttttttga gagaaacagg
    17041 aacaggatgg gtgacagcag aatatagtat gctgcctcga gcaacgagta cgagaaatcg
    17101 tagagaaagc agcaaaggga aactttctgg tagaacaatg gaaatccagc gtcttattgg
    17161 tcgttccttg cgtgcagtag tagacttgga aaaattagga gaacgtagta tcatcgtaga
    17221 ctgcgatgtg atccaagcgg atggcggtac tagaacagca agtatcactg gggcatttgt
    17281 ggcattgaaa ttagccattg aaaaactttt aagagaaaaa gaattgtcag aagatccaat
    17341 aaaagaacat cttgctgcag ttagcgtagg aatcttacca gatgggactt gcgtcacaga
    17401 tttggattac caagaagatt ccgcagcact tgtagacatg aatcttgtaa tgacagaatc
    17461 tggaaaattc gtggagattc aaggaactgg agaagaagca acgtttgatg gtgaacagtt
    17521 gaatgaaatg ctcttttttg gaaaaaacgc gattgaagat ttgatcaaag agcaaaaaca
    17581 tgctttgcta acagagtttg ctcaaaatga tgagcggata gaagaaacaa aaacaatcat
    17641 cattgcgaca agaaatccag gaaaagcaga agagtttcga aatatgttca aagaagcggg
    17701 atatcatgta aaaacattac ttgactatcc tgaattgcca gatgtggaag aaactggcag
    17761 cacatttgaa gaaaatgccc gattaaaagc agaaacgatt gcacaattgc ttgatcagcc
    17821 agtcttagca gatgattcag gtttgaaagt ggatgcttta ggcggtatgc caggaatcta
    17881 ttctgcacgt tttgcagggg aacaaaaaag tgatgcgggg aataacgcca aattattata
    17941 tgaattgaca gatgtgccgg atgaaaaaag aacagcacaa ttccattgta cgttagtatt
    18001 cgcggcaccc aaaaaagaca gccttgtagt agaagcggaa tggcccggtc gagtagcccg
    18061 tataccatct ggcgaaaatg gctttgggta tgatccttta tttatcccag aaggtaagaa
    18121 acaaacagca gcagaattat cgagcgaaga aaaaaacaag atcagtcatc gggctcaagc
    18181 aatgaaaaaa ttgagtgccg agtggaaaca atggctggaa ggagagagat agatgcggta
    18241 tttagtagta agtgataatc atggagacag agatatcctc gttgacttag tagatcgtta
    18301 tcgttctgaa gtggatatct ttttccattg tggtgattcg gaattagaag cttctgaccc
    18361 attatgggat acattccaag tggttcaagg taattgtgat tatggaccag gattcgaaca
    18421 gaaaaaagtg atccaaactg gtcaggatac agtattcatg actcacggtc atttatcgaa
    18481 tgtccgattt ggtttgacac agttagcaat cgaagctcga gctgcaactg ccgatatggt
    18541 gttttttggt catacccatc aaataggctg tgagatggat cagaaaatcc tgtttttgaa
    18601 tcctggtagc atttctcagc ctagaggccc gatccaaatc ccatcatatg ctcttatcga
    18661 aagtttagat gaccaaattt ctgttcaata ctataaccgt gcccatcagg cagttgaaga
    18721 gttggccttt tctttttcga gagagcgctg aataaaaaaa ctctaatttt taataaaaat
    18781 aagctttatt ttatgaattt tgcatccggt ttcggtaaaa tagtagtgtt gaatgaaata
    18841 gaaatggagg ccatcagatg attggaccta tagtaagaga aatgctgctg gaaaatcagg
    18901 aaacgttttt gattcctgca gaaaatgtgg caaatgttat gtatcatcat ccattgtccc
    18961 atggtttact tgtgttatcg aaggtcggtt attccaaaat tcctgtatta ggaaaagatg
    19021 atcactttgt aggactagta agtttatcag atgtggtcaa taaaatgatt gacttgcaaa
    19081 caatcagcat ggacccgtta gaaggactaa cggttgctga tgtgacagaa acagatgttc
    19141 cgacaattga tgaaaattgg gaactagaag aagtactcca tttgttagtc gattcgcctt
    19201 ttttaccggt tgtcacagaa gatcggattt tcaaaggaat cattacaaga aaagagctgc
    19261 ttaaagccgt aaacttcatg gtgcatgaat tagaacgtcg taatatattg tctccgaaaa
    19321 tcgaagaatt aaaagaacaa atcgatatcg tgagctaaag aacgagaaac taaaagataa
    19381 tcaaaaaata agtagaaaaa acagcgtacc agttttgact ttatcttgaa aaagaggctg
    19441 ggacaaaagc gtttaactcc aagaaataag aaggaattca cgaaaattgc ttttcaaatt
    19501 tttgtgaatt tcagcttatt tccgaaggag ttgcttctgc ttccgccgtt tatacgtgtt
    19561 tagagcgtgg gacaaaagtg ttctttactt ttgtcccacg ctctttttag tgagattcgt
    19621 tgcatcgttt gtccaaatta ttttccgaca gcaattggac tgttcggaat cgtgaaacca
    19681 gaagctgtca attctttgac ataggagctc aagaactctt cttttaaggc atattgtttt
    19741 ccgttcaatg cgtagcagac cgtacgaatc gcaaaattgc tgtttccaag atccactaat
    19801 ccaaagatag aaggttcagt ttggagctct tcttggtatt tttcagctaa tgtacgattt
    19861 actcgatcaa tgatctcata gattttgtca tatccttctt caggaacaat ccgaatatct
    19921 aaaagtactt gcatatttgc acgagagaga ttgctgatcg tggtgatgtt tcgattcggg
    19981 atgaaatgga cagtaccatc cgttgctttc agctgaaggg tacggatacc aacagatacg
    20041 acggttcctt caatcgttaa gtttgctagc ttgatgtagt ccccaacatc catctgttgt
    20101 tccatgataa taaaaaatcc ggtaataaca tcactcatga acccttgggc accaagaccg
    20161 attgccacac ctgcaattcc ggcaccagcc aaaagtgatc cgatcggcac accgatagca
    20221 gataaaattg catagataaa aaagaaacca agggtataat gaaaaatagt atttaaaaga
    20281 ctatgtaaag tctttaagcg gctctcgttt agaatggctt ttttagaata ctgattaaaa
    20341 gacttatcaa tcagatattt tcccacacgg tgaagaatcc aaaaaaggac gatcaaaaaa
    20401 atcagatata aacttttttg gataaaggca gccaaaatgg cgtcccaatc aatcccgtcc
    20461 caaaaccgct gaaacgcatt tactttgctg acagcttgat ctgtcaatgt ctcgccagaa
    20521 gagtctgtcg taccggcagt tgctaataat gaaaacataa aatcctcttt tctaaaattt
    20581 gattctttct gtattgtacc aagaagtttt caaaaaagga aatagcaaaa ataactataa
    20641 tgaaggaaaa aagctgaaaa taaaatgaca aataatatgg ataatgataa aatgtggaga
    20701 aaataacgaa ggtggtaagt atatgcaaat cgattggaac aatctaggtt tttcttatat
    20761 caaaactcct tggcgtttta ttgccaaatg gaaagacgga caatgggaag aaggagaatt
    20821 gacagaagat aattacctga cgattcatga aggctctcca gcgcttcatt atggtcagca
    20881 atgttttgaa ggtctaaaag cttatcgtcg aaaagacggc aaggtcaatc tctttcgacc
    20941 tgaacaaaat agtcgacgga tgaatcaaag tgctagacga ttgctgatgc caatggtccc
    21001 tgaaaagatg ttcgttgatg ctgtcaagca agtggtaaaa gcaaatgaag attttgtgcc
    21061 gccctatgga acaggcgcta cattatactt gcgcccttta ctgattgggg tcggtgaaaa
    21121 cattggtgtc catccagctc cagaatattt atttgtcgtc ttctgtacgc cagtaggagc
    21181 gtatttcaaa ggtggactaa aaccaacgaa ttttattgta tctgattacg atcgtgctgc
    21241 tcctaagggg actggtgcag caaaagtcgg cggaaattac gcagcgagtt tacttccagg
    21301 aacagaagca aaagaactgg attactcaga ttgtgtttat cttgatccag ccacacatac
    21361 gaaaatcgaa gaagtaggtg cagcaaattt ctttggaatt acaaaagacg gaaaattcat
    21421 cactccgcaa tcgccctcta tcttaccaag tatcacgaaa tattccttgt tgcagttagc
    21481 tgaagaacga ctaggactgc aagcaattga aggagatatt ttcattgatg atctagatca
    21541 atttgcagaa gctggtgcat gtggtacagc ggctgttatt tctccaatcg gtgggattta
    21601 tcacaataac aatcttcatg tattttatag tgaaacggaa gtaggaccag taacaaaacg
    21661 gttgtatgat gaattgacag gtattcaatt tggagacata gaagcaccag aaggctggat
    21721 tgaagtcgtg gaataatttc aaagtaatga gttgaaaaaa acgtaaaaag aaaaaatcag
    21781 gaagagactt taaaaagttt tttcctgatt tttatatgtt ttactttaaa gaatgcaaca
    21841 aaagtttatg aaagatggac acctttgtca acgtagataa tatcaccgat gattccgctg
    21901 gaaagtgggc tgattaagaa agcacaagca ttaccgactt cttcgatcgt cacaccttgc
    21961 tgatcaggcg tacgagattc agataactgg atcagttttt gatagtcttt aacacctgtg
    22021 acagctaatg ttttgattgc cccagctgag attccattga cccgaattcc tttaggcgag
    22081 aactcagctg ccaaataacg gatagcagct tcaagagaag ctttcgcaat acccatcatg
    22141 ttataattcg ggattgcgcg ttctgaacct agataagtca tgctaacgat cccgctgttt
    22201 ggtgcaagaa acggttgtgc gtattttgat acagcaatta aagagtagct gctgatatct
    22261 tgagctaatt gatagccgtc tctggaaata tcagacacat ttccggacaa ttcttctttg
    22321 ttggcgtaag caactgcatg gacaaggccg tggatctccc cagcatattc accgatggca
    22381 ttcattgctt tttcgatgct ttcgtcgcta gaaacatcac attctacagt gaatgcttct
    22441 tcgccgacca atttgactaa tgattttttc attcgttcat tttggtaagt atagataatt
    22501 tctgcccctt gtttcaacat tgcttcagca caaccccaag caatgctttt tttgttagcg
    22561 acgcccataa tgacgatttt ttttccttct agaaatgaca taatgactct cctttgtttc
    22621 tgataaaaat ttgatagtcg taaaaaaata gagaataata gctaaaataa cttcttgatt
    22681 tcttaattat tagtcaaaat gttttgattg tcaaactatt ttttcttaaa ttaaaaaaag
    22741 attgctaaat atttaggaat atgatacttt gatagtcaaa gtaatgaaaa tggcaattgc
    22801 ctgaaagaag gagtttaatt atgtcttacg tattatctgc tactgaagta atggaattga
    22861 ttcctaatcg ttatcctatt tgttatattg attatgtaga tactttagaa ccaggaaaaa
    22921 agatcgttgc gacaaaaaat gtaacaatca atgaagattt tttccaaggt cactttcctg
    22981 acaatccagt gatgccgggt gcgttgattc tagaaacact ggcacaagca ggttcgatac
    23041 ttatcctaaa atcagaagag tttctaggaa aaagagctta tatcggtggg atcaataagg
    23101 ccaaattccg tcaaaaagtt gttccaggag atgtgctgaa atgtcatttt gaaatcacca
    23161 aaatgaaggg atcagtcggt acagcgttat ctgcagcata tgtagatgat aaaaaagtct
    23221 gtgagtgcga gttcacgttc atcgtcaacc aaaatgcttg actataaaac ctgatatagc
    23281 aaaacaaaag gaagcttact tgctttttat gtaaaaagaa gacgcctgaa tcctagcata
    23341 cgttgtcatc tatgcaggat tcagacgtta tttttttatt tttgttttgc ttcttgcagt
    23401 atattgattt tatcgatttt tacttcttct tttggtttgt ctgaactatc tgtttcgata
    23461 tttgcaatag catccacgac atccattcct tcaatcactt ggccgaagac agtataatgg
    23521 ccatctagtg aaggataacc gccgttttta taagcgtcga tgattgcttg aggatagtcg
    23581 tcttttaaaa gaccatcatg catatcatct gtattttgaa cgataaagaa ctggctgccg
    23641 ttactgttag gatcttgtga gcgagccata gataacgcac ctcttatatt ataaaggtgg
    23701 ttgttgatct ctgtttcaaa tccttctccc caaatgcttt cccctccagt gccatcgcct
    23761 ttaggatcgc caccttggat catgaaatct ttgatgacgc gatggaaagt cagtccgttg
    23821 tagtaaccgt cttttgcatg tgtcataaag ttttcaaccg cttttggtgc gtgttcggga
    23881 aataatttga ttttgattgt gcctttattc gtcaccattt cgaccagatc ttcattttcg
    23941 gcgacatctt tcgtcaattg tggtaattcc aatgcgttca aatcgacgct actggcagtc
    24001 gtgctttccg cagtagatgt actttttgaa ctttctgtag acgaactaga agaagtcgtg
    24061 ttgtttgcag aacaaccagc agcaaacagc aatgctgctg ctaaactaat acttaaaata
    24121 actttatttt tcttcataac tgttcctttc caatttacta ttttgttatc tttaatgata
    24181 acaaaataga gggaaaatac ctacttaaat tattggaatt tatcatttat tttaaaagaa
    24241 aaagaaaagg tgtagaccat ttactttgtg tttgctataa tgtaagtgga tacaaaaacg
    24301 aaaagatgca ggaggaaatg gtaatatggg aaaattagga atctcgattt atccagaacg
    24361 gtcaacattt gaaaaggaca aagcgtactt ggatttagct cataagtatg ggtataaacg
    24421 ggtttttacc agtttgctgc agatcaatga tgataaagaa aaagttcttt cagaatttaa
    24481 agaagtagtt gattacgcga atagcctagg catggaagta atggttgata tcaatccagc
    24541 gttatttgaa cagttggaga tttcttacga tgacttatct tttttccata agatgggtgc
    24601 ctacggggtc cgcttagata tcggattcac aggagcagaa gaagcaaaaa tgacgcgcaa
    24661 tccttttggt atcaaaatcg agatcaatat gagttcgggc acgaattacg ttgacaacat
    24721 catgagttat tcgccaaaca cggataattt attaggttct cataattttt atcctcatcg
    24781 atactctggt ttaggatacg aacactttgt tttttgttca gaaaaattca gaaaatataa
    24841 tttgaatacg atggcattcg tcaattcgca atctgctgag tttggccctt ggcctacgca
    24901 agatggcttg tgcacgctgg aagaccaccg agatcttgaa attgcgacac aagtgaagca
    24961 tttaatcctt acaggattga ttgatgatat ttctattgga aatgcgtacg caagtgaaga
    25021 agaattgaaa gaaatggcag aagcatttaa cgcggattat ccaacattga aagtagatac
    25081 ggaagaaggt attacagaaa acgaacgaat ctgcttgttt gataaccttc atagttatcg
    25141 aggggatcgc tcagaatata tccttcgttc aacgatgact cgtgtatact acaaagataa
    25201 agatttcccg cctcataata cgcgagatat gcatcatggg gatgtactga ttgacaatga
    25261 agggtacggt caatacaaag gagaaacaca aatcgccttg aaggatatga aaaacgacgg
    25321 acgtgtcaat gttgtaggaa gaatctccga tgacgaatta ttcctccttg actttttgaa
    25381 accatggtca agctttaaat taattgaaaa taaaaaatag ataattagtt attgcagaaa
    25441 taatccgcta gaaatagcca gttatttctg cttttttatt ttgttttttt ataggttatg
    25501 atttgtcaaa ttaagctaga ataaaatgct tcttgtacat agctcaaaaa tatttcgatt
    25561 ggtgttctgt aattcaatga ttttcttgga atatgattac gttgattgct gacactggaa
    25621 ataaatgtct gattcacttt tctaaaatcc attgatttcg gcagtccatt acgacgcaga
    25681 atcccgttag aatt
//
LOCUS       DAFEOM010000032        23573 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.033, whole genome shotgun sequence.
ACCESSION   DAFEOM010000032
VERSION     DAFEOM010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.033"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            44..676
                     /locus_tag="LP524_001531"
     CDS             44..676
                     /locus_tag="LP524_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBL2234590.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(678..1583)
                     /locus_tag="LP524_001532"
     CDS             complement(678..1583)
                     /locus_tag="LP524_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBL2234591.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(1668..2327)
                     /locus_tag="LP524_001533"
     CDS             complement(1668..2327)
                     /locus_tag="LP524_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBL2234592.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            2643..3587
                     /gene="mvk"
                     /locus_tag="LP524_001534"
     CDS             2643..3587
                     /gene="mvk"
                     /locus_tag="LP524_001534"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HBL2234593.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            3580..4557
                     /gene="mvaD"
                     /locus_tag="LP524_001535"
     CDS             3580..4557
                     /gene="mvaD"
                     /locus_tag="LP524_001535"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HBL2234594.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            4570..5655
                     /locus_tag="LP524_001536"
     CDS             4570..5655
                     /locus_tag="LP524_001536"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HBL2234595.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            5652..6716
                     /locus_tag="LP524_001537"
     CDS             5652..6716
                     /locus_tag="LP524_001537"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBL2234596.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(6965..7768)
                     /locus_tag="LP524_001538"
     CDS             complement(6965..7768)
                     /locus_tag="LP524_001538"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HBL2234597.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(7788..8630)
                     /gene="kduI"
                     /locus_tag="LP524_001539"
     CDS             complement(7788..8630)
                     /gene="kduI"
                     /locus_tag="LP524_001539"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HBL2234598.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(8646..9653)
                     /locus_tag="LP524_001540"
     CDS             complement(8646..9653)
                     /locus_tag="LP524_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBL2234599.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(9660..10304)
                     /locus_tag="LP524_001541"
     CDS             complement(9660..10304)
                     /locus_tag="LP524_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBL2234600.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(10404..11162)
                     /locus_tag="LP524_001542"
     CDS             complement(10404..11162)
                     /locus_tag="LP524_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBL2234601.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(11289..12440)
                     /gene="fucO"
                     /locus_tag="LP524_001543"
     CDS             complement(11289..12440)
                     /gene="fucO"
                     /locus_tag="LP524_001543"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HBL2234602.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(12522..12833)
                     /gene="rhaM"
                     /locus_tag="LP524_001544"
     CDS             complement(12522..12833)
                     /gene="rhaM"
                     /locus_tag="LP524_001544"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HBL2234603.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(12835..13680)
                     /gene="rhaD"
                     /locus_tag="LP524_001545"
     CDS             complement(12835..13680)
                     /gene="rhaD"
                     /locus_tag="LP524_001545"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HBL2234604.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(13694..14950)
                     /gene="rhaA"
                     /locus_tag="LP524_001546"
     CDS             complement(13694..14950)
                     /gene="rhaA"
                     /locus_tag="LP524_001546"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HBL2234605.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(14947..16407)
                     /gene="rhaB"
                     /locus_tag="LP524_001547"
     CDS             complement(14947..16407)
                     /gene="rhaB"
                     /locus_tag="LP524_001547"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HBL2234606.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            16560..17429
                     /locus_tag="LP524_001548"
     CDS             16560..17429
                     /locus_tag="LP524_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HBL2234607.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(17555..18454)
                     /locus_tag="LP524_001549"
     CDS             complement(17555..18454)
                     /locus_tag="LP524_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2234608.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(18619..19443)
                     /locus_tag="LP524_001550"
     CDS             complement(18619..19443)
                     /locus_tag="LP524_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBL2234609.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(19454..20749)
                     /locus_tag="LP524_001551"
     CDS             complement(19454..20749)
                     /locus_tag="LP524_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HBL2234610.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(20765..21244)
                     /locus_tag="LP524_001552"
     CDS             complement(20765..21244)
                     /locus_tag="LP524_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBL2234611.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(21250..22278)
                     /locus_tag="LP524_001553"
     CDS             complement(21250..22278)
                     /locus_tag="LP524_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HBL2234612.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(22295..23401)
                     /locus_tag="LP524_001554"
     CDS             complement(22295..23401)
                     /locus_tag="LP524_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBL2234613.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
BASE COUNT         6779 a         5200 c         4238 g         7356 t
ORIGIN      
        1 tcccgcttct ttgctatact aaagaaaaac gaggtgaacc cacatgaaaa aaatcgaacc
       61 tattccacct tctcttcctc ttcttacaaa gggagagttt tatttagaag atgaggagat
      121 cttctctggt cttctgatca acgggggtga ccaaagctat ctcgatgcca aaaatatggt
      181 attaagagag agtcatatcc aaaaacttac gatgcaaaaa acagttttac gtcattttga
      241 atgcagtaat gtcatttttg aaaaatgtga tttttccaat ctggagtgga ttggtggcag
      301 tttccaccaa gtcgtgtttc atcaatgtaa attaacaggt acgaattttg ccgaaagtta
      361 tcttcgagac tgtactttca ctgattgtat tgcttcgatg gcttccttta gtagtacaaa
      421 cttgaaagct gtctccttcg atcattgtca attagaagac agcgaatttt atgaggtcac
      481 ttggaaaaac ctttttttgt ctgacaatca gttgaccggt tcgaactggt ttcgaacttc
      541 tctgaaggga ttagacttta cgactaattt cttcacagca atcgctttat ctcaagattc
      601 tttggcggga ctaatcgtca accaagagca agcacttgtg atcgcccaag cccttggttt
      661 gcagatcaaa gactagatca acgaatccgc cgccacagtg caataagcat ccaaatagat
      721 aagaccaaac tgatgatcaa ggcgattccc catccgaaaa cggaatgggt aaagggtaac
      781 tggagattca ttccgaagaa tcctgtcaca atagttggaa cggtcaagat cagcgaccag
      841 accgtcaaaa atttcatcgt gtcattcaag ttgttattca gcaaattatt ataagtgcca
      901 gagagctggt ctaaaatctg cgaagctaag ttcgtcattt caactgcttg tcgtgcttcg
      961 atcagcgcat cgtccaactc ttctttttcc ttttctgaca acttgcgata aatagccaat
     1021 gccttgattt gttctaaaag tactacattt tgttttgttc ctgtaactaa gtaaaccaaa
     1081 ccgatctcta aatctgaaag ttgcagcaga ttcttgttcg ttgttttttc tcttaatttc
     1141 agattaagcc gctgacgttc actattcact tcctcgatta aaggaaaaaa ggaatcagaa
     1201 atcaaaaaca atgtacgaaa caataaactg tatacactca tgtctggcat tcgttccaaa
     1261 agcgatcgaa ttagtcgaat cacgtactcg gtattcggcg tggtaaatgt aaacaaaccg
     1321 tcttctttta aaataaaagc gataggacta gtttcataat gattatcgac tttttgctgg
     1381 tgcggaacat ttgatacaag cagtaacgct tcatcaaaag aatcgtactc tacgcgcgcc
     1441 cgttcatttt tatctagaga ataagtcagc atctcgtcac taatgccata ttcttctttt
     1501 aagtttttaa cagctagcac atcgttttgc tgtacattga tccaaaaatt acggtcttga
     1561 ttaaaatagt gtttttcccg catactctat cctcctgaca tttgtttcca gtataaagaa
     1621 aaaagtcgct caaataaagg tttacccttt atttgagcaa ctgtaaatca aatggctact
     1681 ttggtttggc gaatctctgc taatttttct tcgatcattt tcaagacttc ttcttttgct
     1741 tgaggatctt ccacaaaatt atagcggtct ccatcgattt ggatttttgg actttctttg
     1801 tattcttcat accattgatc gtaacgtctg ttcaattctt tgtaataatc gtatagactt
     1861 ggatcaaagg acaattgttc atacgaacga ccacgttttt cgatacgctc caacatcgta
     1921 tcaaaagata cacgaatatg aactaataaa tccggatgtt ttttatgggc agcataggga
     1981 agttcttcca tcatgttttc cagcagttca tcatacacac gaacttctgt ttctgttgct
     2041 cttcctagat ctgcatttag atggaaaagc agtgaatctt cataaatcga acggtccaat
     2101 acattattgt catctttcat tgcttgtttg atgcttgcaa aacgtttatt caaaaaataa
     2161 atttgcagca aaaaagcata ctgttcaggg tttgcataaa ataagggcaa gacttcatta
     2221 tcatcaatcg attcataaaa tgcctgacta tcaaagtgtt cagccatcat ttctgttaaa
     2281 cttgattttc cggcaccaat tgtacctgct aaaacgatca cgctcattta cctccagctc
     2341 acttcatcac taaatattgt gtttaactcc aaccttttta aatatacccc caatatctag
     2401 tagtgtcaat tgtatttttt tctagtcaaa gaagaaaaaa agtcaaaaaa ttcttaagaa
     2461 aaaagtgttt tttctttaga aaacgtattc ttagttttac ttctgagatg gttttagtaa
     2521 aataaaggag gaatgttttt tgagaccctt agtggacaaa aaaattcgtg aagcagcgca
     2581 actcgttttg ggcgctttat tttttgctta aaaatcactt ttacgtgaga aggagttaga
     2641 ttatggcaaa ctatggccaa ggagaatcaa gcggaaagat catattgatg ggcgagcacg
     2701 cggttgttta tggagaacca gcgattgcct ttcctttcta tgcaacaaaa gtcaccgcat
     2761 tccttgaaga gctggatgca atggacgatc aactggtttc ttcctactat tcaggaaatt
     2821 tagccgaagc tcctcatgca ttaaaaaata tcaaaaaatt attcattcac ttaaaaaaac
     2881 agcatgacat ccaaaaaaac ttgcaactga ccattgaaag cacgattcct gctgaacgtg
     2941 gaatgggatc aagcgctgca gtcgccacag cagtcactcg tgctttttat gattacttag
     3001 catttccttt gtctcgtgaa atactattag aaaatgtcca gctttcggaa aaaatcgccc
     3061 acggtaatcc tagtggaatc gatgcagccg ctactagcag cttgcagccg atttatttta
     3121 caaaagggca tcctttcgac tacttttctt tgaacatcga tgcttttttg attgtcgctg
     3181 atacaggaat caaaggacaa acaagagaag ccgtcaaaga tgtcgctcac ctctttgaaa
     3241 cccagcctca tgaaactgga caaatgatcc aaaaattagg atacttgacg aagcaagcaa
     3301 aacaagcaat cattgaaaat tcaccagaaa cgttggcaca gacaatggat gaatcacaat
     3361 cacttctgga aaagctgaca atcagcaatg attttcttaa tctattgatc caaacagcaa
     3421 aagataccgg ggcattgggt gccaaattaa ctggcggtgg acgcggtggc tgtatgattg
     3481 cattagcaca aacaaaaaca aaagcccaag aaatcagtca agcacttgaa gatgcgggtg
     3541 ctgctgaaac ttggatacaa ggattaggag tacataccta tgtttaaagg caaagcacgc
     3601 gcatatacca acattgctct aatcaaatat tggggtaaga aaaatgaaga actcatcctt
     3661 ccaatgaaca acagcctttc gttaacattg gatgcatttt atacagagac agaagtcatc
     3721 ttttctgata gctatatggt agatgaattt tatctagatg gcaccttgca agacgaaaaa
     3781 gcaacaaaaa aagtcagtca gtttcttgac ctttttcgta aagaagccgg cctctcacta
     3841 aaagcttcag taatcagtca gaatttcgtt cctaccgcag ctggattagc ctcctctgcc
     3901 agcgggctag ctgctttagc aggcgcttgc aatactgctc ttaagcttgg attagatgat
     3961 ctctctcttt caagatttgc tcgacgcggg tctggttcag cttgccgaag tattttcggt
     4021 ggtttcgtcg aatgggaaaa aggccatgac gacttaagtt cttacgctaa gccagtccct
     4081 tccgattctt tcgaagacga tttagcaatg gttttcgttt tgatcaacga ccagaaaaaa
     4141 gaagtgtcca gcagaaatgg gatgcgtcgg acagtcgaaa catccagttt ttatcaaggc
     4201 tggttagatt ccgttgaagg ggatctatat caattgaaac aagcaatcaa aacaaaagat
     4261 ttccaacttc tcggagaaac gatggaaaga aacggactaa aaatgcatgg aacgacttta
     4321 gctgcccagc ctccattcac ttactggtct cctgacagct taaaagcgat ggatgcggtg
     4381 cgacaattga gaaagcaagg cattccatgc tactttacga tggatgctgg ccctaatgtc
     4441 aaagttttag tggaaaacag ccatttatct gaggtacaag aaacatttac caaattattt
     4501 agcaaagaac aagtaattac tgcacatgct ggtccaggaa ttgcgattat tgaataaagg
     4561 gagattttta tgattgaagt atctgcacca ggcaaactct atattgccgg agaatatgca
     4621 gttgttgaaa caggccatcc agcagttatc gctgcagtcg atcaattcgt gacagtcact
     4681 gtagaatctg cacgaaaagt cggaagtatc caatctgcac aatatagtgg gatgcctgta
     4741 cgttggacaa gacgcaacgg agaattagtt ttagatattc gggaaaatcc ttttcattat
     4801 atccttgctg ctattcgctt gactgaaaag tatgcacaag aaaaaaacat tcttttatcc
     4861 ttttatgatc tgaaagtaac aagtgagtta gacagttcaa atggccggaa atatggattg
     4921 ggttctagcg gtgccgtgac cgttgcaacg gtcaaagcct tgaatgtttt ttacgcgttg
     4981 aatttatctc agttggagat tttcaagatt gcagcactag ccaatttagc agttcaagat
     5041 aatggttcct gcggcgacat cgctgctagc tgttatggtg gctggatcgc tttctcaacc
     5101 ttcgaccatc cttggctcca agaacaagaa actcagcatt ctatcagtga gttacttgcc
     5161 ctggattggc caggtctatc cattgagcca ttgattgctc ctgaagattt acgtttattg
     5221 attggttgga cgggtagccc tgcctctact tctgatttgg tcgatcaagt tcaccgttcg
     5281 agagaagata aaatggtggc ttatcagctt ttcttaaaaa atagtacaga atgtgtcaac
     5341 gaaatgatca aagggtttaa agaaaataat gtaacgttga tccaacagat gattcgaaaa
     5401 aaccgacaat tactgcatga tttatctgca atcactgggg tcgtcatcga aacgcctgct
     5461 ttgaacaaat tgtgtaattt agctgaacag tatgaaggag ccgcaaaatc ttctggtgca
     5521 ggtgggggcg attgcggaat cgtaattgtt gaccagaaat ctggcattct tcctttaatg
     5581 agtgcatggg aaaaagcaga aatcactcca ctgccgttac atgtctatag cgatcaaaga
     5641 aaggaaaacc gatgaatcga aaagacgaac atgtttcact agctaaagcc tttcatgata
     5701 aacaaaaaaa cgaatttgac ttcgtacgga tcatccataa ccctttgccg caaatagcag
     5761 tttctgatgt cgatctaagt acacaggcag ttgggtttac actaagcagc ccattttata
     5821 tcaatgcgat gactggcgga agcgaaaaaa caaaaaaaat caaccaagat cttgcaatcg
     5881 tcgcaagaga agcagatttg atgattgcca ctggttcagt cagcgctgcc ttgaaagtcc
     5941 cttctttagc cgatacttat acgattatgc gacaagaata tcctcacggg aaaatcattg
     6001 ccaacatcgg tgcagggact tctgtcgaaa gagccaaaga agctatccga ttgtttcacg
     6061 cagatgcctt acagattcat ttgaatgcac ctcaagagtt ggttatgcct gagggagacc
     6121 gtgattttac caattggaaa gtacttattc aagaaactca aacagccatt gacgtccccc
     6181 tcatcgtaaa agaagtcggt tttggcatga caagagaaac gctcaatgat ctagctgctt
     6241 taggagttca tacagttgat atcagcggtc gaagcggaac aagcttcacc cagatcgaaa
     6301 acgcccgtcg ctccaagcgg gaactgagtt atttagctga ctgggggcaa tcgacagtct
     6361 cttcattgct tgaagcaaat gaagcagaca cttctatgga aatcctagcc tccggaggta
     6421 tccgcaatgc ctatgacata tttaaagccc tttgtctcgg cgcaaatgcc gtgggtactt
     6481 caggaaccgt actgacgcat ctgatgaatc acggcgtaga agaaactatt atactgatga
     6541 agcaatggca agaagagctt cgtctgcttt atactatggt cggcgcaacg aataccgcgg
     6601 ccctacacca acaatctctg atcttttccg gaccagtgaa ggattggtgc gaagcaagag
     6661 ggatcgactt agtgaagtat ggaagacgca cagaaaagag cgtgggacaa aagtaaagaa
     6721 cacttttgtc tcacgctcta aacacgtaca aacggcggaa gtaaaagcaa ctccttcgga
     6781 aataagctga aattcacaaa aatttgaaaa gcaattttcg tgaattcctt cttatttctt
     6841 ggagttaaat gcttctgtcc cagcctcttt tttcgaataa atgatgttta atcagctgtc
     6901 cttcggtatt cgttcaatcc gctgatttca caacctctcc actaaacagt gtactaaagc
     6961 tcttttaagg ttgttttccg atataggcaa gtatgcctcc gtccacatac aagacatggc
     7021 cgttaacgaa atcagatgcg ctagaagcca agaagacaga tggtccagcc aaatcgattg
     7081 gctcgcccca gcgtgctgct ggcgtacgtc caacgataaa ttgatcgaat ggatgacgtt
     7141 caccgttttc ttgtgtttcg cgtaatggag cagtttgcgg tgtagcaata taacctggtc
     7201 caattccatt acattggata ttgtattgac cgtattcaga agcgatgttt ttcgtcagca
     7261 tttttaatcc gccttttgcc gcagcataag cactgaccgt ttcgcgtcct aattcactca
     7321 tcatggagca gatattgatg attttaccgc cgcctttttc gatcatatcc ggaataactg
     7381 cttttgaaac gatgaatggg gcatttaaat ccacgtcgat tacttggcgg aagtctgccg
     7441 catccatttc gatcatcggt gtccgtttga tgatccctgc attgttgact agaatatcaa
     7501 ctgaacctac atcttctttg atctgttgaa ccatcgcttg aaccgcttct tcatcggtca
     7561 catcgcagac ataacctttt gcatcgatgc ctgcttcttt ataatgcgct ataccttcat
     7621 caactgattc ttgattcaag ttattaaata cgatttttgc tcctgctgta gctaaagagc
     7681 gtgcaatctc aaatccaata ccatagacag caccggtgac taatgctact tttccatcta
     7741 aacgaaacat atccatacta aattccatca ttttctctcc cttatgctta ttttaattga
     7801 tccatttgca ccatatccat gtctgtatag gtgatatttt ctccgcacat tgcccagatg
     7861 aatgagtaat tgcttgtacc tacacctgaa tggatcgacc agcttggcga aataatggct
     7921 tgttcattgt ctacaactaa atgttttgtt tcatctggtt tgcccatcat gtggaacaca
     7981 cgtgtatctt cattggcata atcaaagtag acataagctt ccatacgacg ttcatgtgta
     8041 tgacaaggca tcgtattcca agcgcttcct ggttctaaaa tcgtataacc catttgtaat
     8101 tgacagcttt cgcatacatt tggatggatg tattggtaga ttttacgttc attcagtgtc
     8161 aggccttcac cagtctccat tggttttact tggtcgatac tgatttttac atttggatat
     8221 ttatgatgag ctggaacaga actaatatag aatttagctg ggttttcagc atcttgagaa
     8281 gaaaatacaa catgtcgtgt ttcttttccg atatagtatc cgtcttgttt tttcatttct
     8341 tctttttgcc catcgatctc gatgaatcct ggtccaccga tattgatcac acctagttca
     8401 cgacgctcta agaagtaatc aacacctagt tctttgtcta atgtgatttc taattctttt
     8461 tcagtaggtg tcactccacc aaagatcatt cgatcgttgt gcgtataagt caaactgatt
     8521 ttccctggtt caaatacctt ttctactaaa aattcttttc tcaattgttc cgttgaataa
     8581 tgacggatat cttctggact gtgtgtataa cgtgtttcca tttcttgcat tttcttctac
     8641 tccttctatc gaatgatttt tccagagccg catgctaaaa attgtgcgac ttcttcttga
     8701 ctaaattggt tgcagtcccc atggatcgta tgcttcaagg cagaagctgc cgtagcaaaa
     8761 tcaatgattt cttgcggaga catattggat aaaatcccat ggagcacgcc acctgcaaaa
     8821 gcatctccgc cacctactcg atccacgatt ggggtgattc tatggacttg ggattccaca
     8881 tattcatggt tcatccacaa tgtccccttc aattcatttt cacttgctga atgaacggta
     8941 cgcttggtcg aataaaacac ctgaatattc gggaactttt cctgcatttt ttgataataa
     9001 taaattagag gttgttcttc tgcttctggc gctttctcga tacctaacaa ataaagagca
     9061 tccatttttc cagctgagca gatatctact aaaggtaaaa gctgatggat tacttcgctg
     9121 gcttcttgct gtgtccataa ttttccacga tagttgatat cgaagctgat caggcagcca
     9181 gctttttttg cagcttctat cacctgtttc gtcatttttt gccatttttt agacaaagct
     9241 ggggtgattc ctgaaacatg aaatatatct attccttgga ataactcctc tagcgaccat
     9301 tccaaatgat ccatttcagc aaaacttgat ccggcacgat catagatcac tgaggcggcg
     9361 cgttcgccac taccagtttc catataataa gtacctagtc ttgatccacc agataatacc
     9421 tgtttcgtat ctactccata acattgcaag tgctttttga ctgcttttcc caatgcgtta
     9481 tcaggtactt ttgtagcaaa aatcgcttca tgtccgtaat tagctaaaga aattgctacg
     9541 tttgcttccc ctccgccata atgggcagcg aatcgttcac ttgaatcgat acgggttccc
     9601 tgctctgtgg acaatcgcaa catgatttct ccgagtgtca ctacttttcc cataagcagc
     9661 tacctcttga tttctttcat ttttgctgca tactgttgcg cgacttccgt tactttgtca
     9721 aaatctcctg tagctgcggg tgctaaaagg ttcccaccta ctccgactgt tacgacacca
     9781 gcatcgaacc actctttcat attgtccaga ctcacaccgc cagttggcat gatattgact
     9841 tgaggcatcg gcgccttgaa ggcagaaatg atacttggtc cataagcgct tccaggaaat
     9901 agtttgacga tatctacccc gcttttcagc gctgttttga tctcggtaat agtcatacat
     9961 cctggtaagt aaggaatttg atacagattg cacatctctg ccgtttcttg atcaaaggaa
    10021 gggctaacaa tatactctgc gccagctaag atagccaagc gagctgtgac agcgtctaat
    10081 actgtgcctg cgccaatcac tatttctggt tggtctttgt agaatgacaa aagttctttg
    10141 attacttgat ctgcttgagg gactgtaaaa gtcaactcaa taccagttag accacctttg
    10201 atgattgcgt ggcaagcatt caaagcttct tctttgcttt ttccgcgtac caccgcaatc
    10261 actccggcgt ttttcaatcg agataaaata tcaatttttt tcatcttctc cacctatttc
    10321 ctatttagaa attttatttt ataacttgga aatctatacc catattatac ggcagaaaag
    10381 aaaaattgca aacgcttttt tcattatttt tgaattcttt gctcgatttc gctttttgtt
    10441 tgcttgaggg cactgattat ttgttctttt gtctcatctg ttaaacgata tttcggcata
    10501 cttacactaa atgcaccaat gatttgttcg tttttcacga ttgaggctcc tatacaaaaa
    10561 atatcacgtt cgatttcttc atcgtcaaat gcgatgtggg tttcttttac cgtttggatt
    10621 tcctttttca accgtaatgc attggtgatc gtatattctg tgtaagggat caacggatgc
    10681 ttttcaagat aatcagtgta ctgctcttca ctgaattctg ctaatacggc tttgcccatc
    10741 gcagaattgt ataatggtcg ggtgattccg atttttgaag acatgcggat cgtttgatgt
    10801 tttggatcta gtttgttcac gtacaagatc tcattgttgt ccaatacccc taagtggata
    10861 gtctcatcga tcttttgctg cagctgttct aaaaacggta aagtcaactc taccaaatcg
    10921 atttgttcaa tgtttttatt cgcgtagcga ataagctttg cacctaaacg atagtttttc
    10981 tctgaatttt tatttacgta gccgatcatc aacaaagtat ctagtatttt caagactgta
    11041 gaagaggtca ttcccacacc ctttgcgatc gtctgcaaag agacatcttg gttttccgct
    11101 aaatagtcta gtattttaga ggctttgatc aatacagttc cataaggctt tttattttcc
    11161 atataatatt ctcctatttc tttataagaa aaattatagc ggaaatacca tcaatttaaa
    11221 agagatatcc agatataaac taccatttct ctttacaggc aatttaatgt tgaagatccg
    11281 cttcgttttt acatcaaact ttgatataaa gcaatgattt cttctactgt cgtttctctt
    11341 ggatttcctg gtgtgcatac atctcgcaaa gcatcttctg caattgctgg aatatctgct
    11401 tcttttacac ctaattcaga aatggtttct gggattccaa ccgctttgct caaccgatcg
    11461 atctcaccac aagccgcttc tcttacatct tctaaagaca tttcagctgc tcctttaatc
    11521 cctagaacaa gagcgatctc acggtacttt tctccagtat attctttgtt atatttcatg
    11581 acagttggca aaagtgctgc acacgctacg ccatgaggaa tgttgtacca tgcacttaac
    11641 gggtgagcca tcccgtgtac cagtcctaaa ccgacatttg agaagcccat tcccgcaata
    11701 tactgtccta atgccatttt ttctcgccca ttttgatcgc catctacaga agcttccaaa
    11761 gaatggccga tgatttcgat tgcttttaaa tgcagcatat cagtcatttc ccaagcgcct
    11821 tttgtgataa acccttcaat agcatgcgtc atagcatcca tacctgttga tgcagccaat
    11881 tttttcggca ttcccatcat catatcgctg tcgatgaacg cgacgattgg gatatcatga
    11941 ggatcaacac aaacaaattt tcggtgattt acttcatctg tgatgacgta gttgatcgtt
    12001 acttctgctg ctgttccaga agtcgttggt atagctaata taggtaaaca tggatttttc
    12061 gtatcagcaa caccttctaa actaatgacg tctgaaaact ctggattcgt caaaataatc
    12121 ccaatggctt ttgccgtatc gattggcgaa cctccaccga ttgcgatcaa acagtctgcc
    12181 tctgcctctt taacaaaagc cactccatct tttacatttt ggatcgttgg attaggtacg
    12241 atcccgtcgt ataaagcata agcgattgct gctttatcta gaagttctgt tacttttgtt
    12301 gctataccgt gctcgatcaa tcctttgtca gtaatgacag ctgctttgcc gaatcctcgc
    12361 tgctgaaatt cagggacgat cgactcaatc gctccttttc caaaatacga tgtttcattt
    12421 aaaatcattc gattactcat tttctattcc tcctaatttg tattattttt cacaataaaa
    12481 acgaaagaac cccaagcaac cggttgccca gttgcttgtt gctacagttc aaagactggc
    12541 atcaattccc aactgactgg agaggcatct ggatttgtct ccatgatatc ttccatgtac
    12601 ttccaccatt tttgattgat agatgtattt gccatttttt cccataactt ttcatcttcg
    12661 atcactaagt aaccaaataa caaacttgta ctcggatcaa gatagatcga atagtcgatg
    12721 accccgtgtt cacgaagtgc tgtacgcatt tctggccaaa gttcatcatg tctttttttg
    12781 tattcttctt gtttttctgg gtaaactttc atgcaaaaag cttttttcac catgtcagac
    12841 acctgctttc gtatgaactt cctccaaata acctgctttt ggagtgacac caaacgcatc
    12901 tgctaaatgc cacaggtttt cgtccgtgat tgtttgcaag attggtcctt gcgctttgac
    12961 gtacgtatag acttctgctg ccttttctgc tgtctcgatc aaaccaaaga cttcatccat
    13021 gtcttttcct gacccgtaga ttccatgttg tggccataaa accaatcgag tttctttcat
    13081 tttttcagct gttgcttccc caatctcatt tgttcccgga accatccacg gaatgatgga
    13141 aattccttct ggaaaaacaa ctaagcattc cgtacacatt tgccatagcg tacgactaaa
    13201 ggaacgttcg tctaattcat gcgtaaaact catcgctaat agatgactag catggcaatg
    13261 cataacgatc cgattatctg ggtctacttc taaacgggcg atatgactca tgaaatgact
    13321 tggcaactcg cttgtaggaa ggcattcgtt ttccagcccc cacaaaagtt ccagtgtttt
    13381 gccatcttca gcaactcgaa cgattccaag attctcggca ggttcgctcg ctacattttt
    13441 aaaatatctt cctgaaccag ttacaataaa atagcatcct gctaaacggc tggcatcaaa
    13501 aatcatcgag atgcggcgga tcactttttc aggatccaag aacggtgtga tctcttcttc
    13561 ttttagtaaa taagaaatat tcccgccatt tctttcatcc catcccaagc gataaagatt
    13621 cgacgtcgtt tcgatcattt ctttcacata aggcgcttgt aaaatatcta tttttttcaa
    13681 tatagccaca ctcctatttt cttaattcca acacgtcttt ttgatattcc tggacttctt
    13741 ccagccaatc caaaccaact ggaacttgat ttttgtaaca gaaataattc catacatcgg
    13801 catacgggaa atctttcaat tcttccgtat aagccattct tgaagtaaag tcaaaagcca
    13861 gctccatttc tttcaatcgc tcaactggtt ccagcatagc tttcagcagt gctttttgtg
    13921 tatttctcgt accgatgacc caagcagcga tacggttgat cgttgcatca aagaaatcca
    13981 gaccgatatt tgttttttcc aacaattcgt tacggactaa ttctttagcg atttcctgca
    14041 attcatcatc catcaaaacc acatggtctg aatcccaacg cactggacgg gaaacatgca
    14101 acatgattcc tttgctgaat aaagctaatg aagataattt attggaaatc acttctgttg
    14161 gatgaaaatg tccagcatct agacaaacaa gtttgtcacg agtcaacccg taacccatat
    14221 aaaattcatg ggaaccgact gtataagctt cagcgcctaa accaaatagt ttgctttcta
    14281 ctgcatcttg tacataccgt tcatctaact cttctgtaaa aatttcatcg agtgattcca
    14341 ttaaacgttc ccgtggggta aaacgatcaa tcggattatc tttcatccca tcaggtaccc
    14401 aaaagttatt gatacttgtt tgcccagttt ctttcccaaa gtactccgcg attttgcggc
    14461 ttcttttccc gtgttcgatc cagtattcac gaacttcttt atctggatga gccaatgtaa
    14521 agccgtcttt catcattgga tgcgaaaaga aagtcggatt gaagtcaaga ccgatttctt
    14581 ctttttttgc ccactctacc catttttcaa aatgtttcgg ctcaatttca tttagatcga
    14641 cagattcttc tgtatccaaa tagatagcat gaaggttcaa tttgtgtttg cccggtatca
    14701 acgaataagc cttctccaga tcttgtctaa gctcttcagg tgttcgtgcg gctcctggaa
    14761 aattccctgt cgccatgatt ccaccggtca attcgccatc cggtgtcaag aaacctttga
    14821 catcatctcc ttgccagcaa tgcatagaaa ttttgatgtc ctgtatcttt tctagtacag
    14881 catctgtatc tacaccgata gttgcatatt tttcttttgc ttgttcgtaa tttctttcaa
    14941 tcgtgttcat acgattactc ctttgatttt attcacgtac cattccatcg tgttttgctt
    15001 attttcatct gggtaaaaag tttcacatgg aaacgactgt ttgattacat ttcttgcttg
    15061 ttctatattc gaaaactgac ctgtactgag catttgcatg gcaacattcc cgattgctgt
    15121 ggcttcacta ggtcctgctt ctacaatgat tccggcataa tctgctgtca attgattcag
    15181 tagacgatta ttcgatccgc cccctacgat gtgcagcttt tccaatttcg ttccagttaa
    15241 gtgttctact ttgatcagct cttgtgcgta acaaagagca agattatcat acacacaacg
    15301 agcgatagct cccgatgaga cagggactgg ctgctctgtt tcaaaacagt attgctgaat
    15361 ctcttctatc atatttgggg gattcaaaaa tcgagggtca ttgatttcca tcacttgctg
    15421 cagcggtttt tcttttgcag caagctctgc caattccgca taactgtact cgtactcttg
    15481 cattcgggca acttcctgga tcaaccacat ccccatgatg tttttcaaaa aacgcaccgt
    15541 gttatgggca ccccattcat tcgtataatt ttcagaaaaa gcattgcgag aaacattggc
    15601 gatctttgtt tcaatcccta aaagcgacca tgtcccactg ctgatataac cccaacgttc
    15661 tccttgacct ggcgttccta atacagctga cgccgtatca tgacttgcta ctgtgataaa
    15721 ggtcacatct ggcaaatcat attttgaaaa ttgttctttt tgcagatctc ctagtatcgt
    15781 tcccggctct gtcaatggtg gaaataattc cttcgagaca ccgatttttt caagaagttc
    15841 tatttcccat tcttttgtcc ctgcattcag cagctgcata gtagatgcat tagttttctc
    15901 cgtcaccatg ttaccagtaa agacataccc taaataatca ggtatcagta acaatttatg
    15961 tgcttttgcc aaattttctt tttcttcagc aaacaattgg aacaaggtat tgaaaggctg
    16021 gatctgaatc cctgtctgct catacaactt atctagagaa tagatttcag aaaactgttc
    16081 tactgctcca ttggtacgct tgtcccgata agcaatcgga tcatttatcc gatccccttg
    16141 ttcatcaatc agacagtaat cgactgccca tgtatcgatc cccgcaaaaa ggcgatcaat
    16201 ccctgtctgc tttaattttt caaatccaat caaaatctct ttgataagat aatcgatctt
    16261 ccagcgatcg tgaccgtctt tgttccgaaa gccgtttttg aaccggtgta cttcttccaa
    16321 gatcaaagaa ccttttgcat ccctcgtact tttcattagt cgtccgcttg atgcaccgat
    16381 atcgatggca acaaatgttg gattcatttc tccacctctt ttactttatt gttgattagc
    16441 actttattaa taaagcgtta tcatttatta acaatgctat cataatacag aatcttatat
    16501 tttgcttcac actattttgg tatataattg cattatcttg acaagttggg agtgtttaca
    16561 tggattactg ggaaaaagcc aaccactcat ggtcggaaga ctcaaaaaga gtgatcttga
    16621 cgcctacaca aagaagcagg aatttatttt tttatatcca agaaatcggt tacttcaaag
    16681 caccaatccc ttactatact gaacgagcac atttaccttc ttacttgata aagtttactt
    16741 tgtctggcaa aggttcgctt acatataatg ataatcagca acagctaaaa gctggagact
    16801 tattttttat tgactgccaa gaatatcagc gttatcaaac gatcagcgaa gaaccatggg
    16861 aaatggattg gatccatttg aatggtcaga acgtccaatc attttatcag gagttcacaa
    16921 aagacggcca taacaccttc cattcaacag gggatccatc agaaaacaag atccaccatt
    16981 tgatcacaga attgatccac atgcaacaag gcacgaatgc ccgtaccgat ttccagtcct
    17041 ctgtagctat ccacgagttg ctgaatgaac tgattttgca aaagtatcag ctagatttcg
    17101 aagaagagga tattcccgcc tacgtttaca aactgaagca atacttagac gaacatttca
    17161 aagaaacgat tacacttgat caattagaac aacaattctt attgaataaa tatcagctga
    17221 gcaaagattt ttccaaattt atcggttctc caccaatcga ttatttgatc agcaaaaaac
    17281 tttcttacgc taaagacctt cttcggtata cagaatttac ggttcgaatg atttccgaag
    17341 aaatagggat tgagaatttt gcttacttta gccgcctgtt caaagcaaaa acaggactat
    17401 cgccgagaga ctttcgtaaa ttcagctgat tcatataata tgtacgtcat tttttcaaaa
    17461 tattttgaaa tatatagata aaaataaaat aaccgcagtt tctgcttcta tgtaaacaac
    17521 agcagctact gcggttattt tttagtgatc attctcattg ttcatcttca aaatgttcat
    17581 aatgaaccaa ctgtttctgt gtatcacgtt tcaaaaattc tttaggcgtt atgccgaact
    17641 ctctcttgaa tgctttcaga aaatgcggat aagaagaaaa accactttta ctatatacag
    17701 cactagagct gcggtattct cgcaacaagt gcttggcata gatcagcttt ttcttcatta
    17761 catactgatg aaacgtaaat ccagtgtact ttttaaattc tcgtgtgata tggtatttgc
    17821 tgacaaaaaa ctgcttttcc atttcctcta aagaaagcgg atgagataaa ttttttgaga
    17881 tatattgtaa catttgttcc acacgctcat taggaactac agcattctgt tcttgcgcac
    17941 tctcttcttt ttgtacaact tgattgagaa aaatcaagta ttctaccaga gcttgttcat
    18001 attttatgtc ttcaccgtaa atttttttat caggaggatg gtcgataaat gataattttt
    18061 gcttcaatac ttcctgttct atttgcaata cacgactcct cttctctccc attggttgaa
    18121 agcaagccga tagattagac catggcgtgg aacgactttc tagaaaagaa ggggtgacaa
    18181 agatatagac acgctcgaat acatctgaag attggcggac gatacgatgc aaatcattgg
    18241 aatggatgag cagcacactt cctgctttga tcgtaaattg tcgtccgttc aaataaaaag
    18301 tcgcttctcc ctttaatgta gcatggatct catagaaatc atgatgatga tagatcgttt
    18361 tattttggct gacgacatct ttgacatgat agatttcata ctgatcagaa accatcgtat
    18421 tgacttgttc accgctgatt ttcttttgga gcatgtgccg cacctccttt tgttcactta
    18481 tgcacaattt tattatacca cgttttttgt aatcaaaaag agctgctttt atatactctt
    18541 ataaaaaaga agagacgtgg catcttctag ccccgccccc tccattctaa atattagtta
    18601 accaacgtct tctttacatt attcaagaag acttcttcca taacgaatcc catcatcttt
    18661 tgtttcttca agaaccacat acaggtacgg acgattcttc atgatgattt ctttgatttt
    18721 tgtgtactcg atcacaccgt ctcccaaatt taccggcacg attttttcct gttcgacacg
    18781 aaaatctttc aaatgaaccg cacatatctt ttcaccaaaa cgttcaaaag cttcttctac
    18841 caattggact tgttcttgat aatcactact tgtgatcaaa ttggtcggat ctaaaatgat
    18901 tcctagatag tccgaatcta ctgcttggat cagctgctct acccttgcta aagaatacac
    18961 cggatgattc aaacctggtt ctatccccac catcatttgg tatttttctc cagcttttac
    19021 caagcgacgg ataacggaca cagcttcagc aaatgcttca tctgtgaaat tttcttccgt
    19081 atattggatt tccggcaaaa cacatccagt ttccgaagca accatcgacg caccaaagga
    19141 agaagcatag cgaagatact gctcgaactt tgtcagcaac tcttgtcgta tcgttaaatc
    19201 tggatgaatc atattgatat agcaacttaa aattcctacg gtcacttctt gcttttctaa
    19261 aactcgtttg acataatttc ccatcccaga attgatctca tttgcgccag aaggtatttc
    19321 aggaaacgaa agacctaagg cgagctgcag tgtatgaatc ccttgttctt ttgttttttc
    19381 tgcaagatct tctatcgttt ggacttcgga aagatcatgt cctctaattg taatgttcaa
    19441 cataactatt ctcctaactc agcatattgg gtagccacat gacgatttgc gggaagtaag
    19501 taatcaaaaa caagacgact aagataaccc cataaaaagg aagaagcggt ttcagcattg
    19561 cctctgcttt tacgcctcct acacttgcac cgacatatag tcctgtacct actggcggcg
    19621 tgatggagcc tacacacaga ttcatgattg aaaacaaccc gtaatgtaca ggatcaaccc
    19681 ctacacttgt gacgattggt aaaaatattg gtgtaaaaat caagatagca ggcgccacat
    19741 ccataaacat acccaccagt aataacacaa gattgactaa taacagaata ataaatttat
    19801 tatccgttac gcccataatc aacgaactaa ttgcctgagg aataccggta aacgccatgg
    19861 cgaaagacat catggaagaa gtagccaata agaacatgat cgtccctgac attgctactg
    19921 attgaaacaa catattaggt atatctttta gttttaccgt tttatagtaa aacatagcaa
    19981 tcaacaggga gtaaatgaca gctatcgctg aagcttctac tgctgtaaat atccccgtca
    20041 agataccacc gataatgatg acaatcaata gaaggctagg aatcgcctct tttattattt
    20101 ttccagcatg agccatctgt cctttttcga caacagggta tttattcttt ttagcaacga
    20161 tgaatgctac tagcatgatt cctagcgccc acaaagcgcc tacgagataa cctcccataa
    20221 acaaagcaga gatcgaagca ccaccagcaa caagtgaata catgataaaa gcagtacttg
    20281 gaggaatgac catacctgtc ggagctgacg cgatattgac cgctgtcgca aacttccggt
    20341 catatccttc ttccacttgc tgagggatca acactccgcc aatcgcggta gaagcggcaa
    20401 tggccgagct agagatggaa ccaaataaag cattccctaa cacattcgta tgagccaaag
    20461 cccccggcac gcgcccgacg aacaacatcg caaaatccac tagtttctta gctatccctc
    20521 cattattcat gataatgccc gagagaacga aaaacggaat agcgatcagc gaaaaactat
    20581 tgatacttcc caccattttt tgagctgaac taaaagcaga aacatcaaac ggtataacta
    20641 acaataaagt catcatcgat gagaatgcaa tacttatagc aatcggcatc cctaatacaa
    20701 gcaacacaac aaatacaaaa aataacacta atccggcttc tattgccatt ttattcctcc
    20761 atttttatat tttttcttga attgctggct tgttgcttaa tgtatcgatt agattgatca
    20821 ggctatagat aatgatgagt acccccgata caggtactga cagataaaca tatcccatag
    20881 gaatacttaa agaaggcgag atctgcccca ttgtcaatgt gacagcattc catccgccat
    20941 acaccatgat catcgcagca aataccagaa ataaactttg aacaacaact tctaaaattt
    21001 tacttttttt gccagttaat ttttcactta aaaccgttac ggctaaatgt gctttttgtc
    21061 ccacaacata cgcggaagac aacatggcaa accagacaag accgaatcga acaagctctt
    21121 ccgtgaccgt atttggattt cctaaaaccg ttctggaaaa tacttgccat aaaacaacag
    21181 cgaccatccc aacaagcaaa acagctccca ggatctctaa taccttgttt aaaatcattt
    21241 tcatcacagc tacgctcctt gctcttggat cttttgataa aggtctttgt aggtaggatc
    21301 gtttttgaat atatcatgaa gtggggcaac tgcctctcga aacggtgtat tatctacatc
    21361 attgaattgg actccatgtt cttcttgtgc taaagctttt tcttcttcaa ttgcttttgc
    21421 aaaagcttca atttcaaaaa ccgttgattc tttcgctgct tcttcaatgg ctttttcctg
    21481 ctcatcagtt agctgttctt tgatggcatc attcatgatg atgatatctg gcacacgtgt
    21541 atgctcatca taggaatagt atttagccac accaccatgt ccagctgtat acaagacaaa
    21601 ttcattgttt tctgatccgt tgatcaaatt ggattgaaga gacgtgtaca cttcatcact
    21661 tcccataggt acgggcgaac cgcctaaaag ctctaccatt ttgatagcgg tttcgctttg
    21721 catcacacgg atcttctttc ccttcaaatc ttccggcgta tggatcggac cgtctaccat
    21781 ataaaagctg cgctgaccag aatcataata ggtcaatccg gtaaatccta aatttttggt
    21841 cgattggtag attggctcca ttatttcttc attatccatc accttaaaga aatgttcttc
    21901 atcattgaat agataaggaa ctgaaaaaat cgaataatct ttagagaaac tttccaacgc
    21961 tgaggcactt acttttgtga aatctactgc tcctgactgt gtcaattcga tcagttcacg
    22021 ttctccaccc agttgtccat ctgggaaata ctggatctgt acggaaccat ctgttttttc
    22081 ttttactaaa cgcccaaatt cattcattgc atcaattact ggctggctgt tgcttgcata
    22141 agcaaagcgc aaggtaacta tctcatttac atctgcatct gcgacagttg ccacactaga
    22201 attatttccg cagccgctta gacctagcag acctaggcta agcgaacttg ctgcaaacag
    22261 cagtgatgtt tttttcatat gggtttcctt tcttttaaca ataagagacg agcaattctg
    22321 tcaaacaaag caccgttaac gactgtccat aaggcattgc tgtgattccg atattccgat
    22381 aaaaatctaa actgtccccc attcctgtcc cgattgaaac gttttgaact tctccttttt
    22441 catcgatttg ttcaagcaat ccttggatag cacgataagc gacttcttta tattcttgtg
    22501 gtaaataacg tttatggacc gctttcaaaa taccgtaagc aaatcctgca gttgcagaag
    22561 attctacgta agaagaagga tcatccaaca acgtatgcca taaaccgctt tcagactgat
    22621 aagtttttaa tgcacgcact tgtgcttcaa gtgtttcgat caataatttg cgcaatgcat
    22681 cttctttcga taattctaag atttcaatga tctctggaat agcaatcgtc aaccagcagt
    22741 ttccacgagc ccaaaatgct tcggcatagt tatgatttcc ttcaaacgtc cagccgtgat
    22801 accacaatcc tgttcgttta tccatcaagt atttgatatg gatcataaac tggtaacgag
    22861 cttcttctaa atattctggg cgattcaaca gtttcccgat ttttgccaaa ggaagcactg
    22921 tcatcatcaa tgtatcgtcc cacaactgat tcttattttc cggtccatat gtggcatgct
    22981 gtaacccgtc ttcatttgtc cgaggcatgt cattcatgac ccattctgcc cattgttcta
    23041 aataaggcaa atagcgtgaa tctttcgtgt cttcatacaa gtaagccata gtcaatagcg
    23101 gtgccattgt attgacgttt ttgggcggag cgccttcttc catacgatcc tcaaaccatt
    23161 cattgaccac ttgataagcg cgaggatttt ttgtcatctg gtaattttta taaataccat
    23221 atagaccgac accttgtggc caattccaca ctgtccagct tttgtcatct actttcagtc
    23281 catcaaagtc caacaaaaat tcaccattat cgtctttgat cgttgtcagg ttttcaacga
    23341 gcaggtcaat tttttctttc actaattctt taggtacaaa aatctcttct ttaataatca
    23401 tctttacgcc acttttttaa acgctttcat tttatatcct taatatagca gtaataccgt
    23461 ccactataaa tatcgctttt tgaacaaaaa ctgtctaaaa ttgacctaaa tgttaaataa
    23521 caaaagtaac tttcagttac ttggaaatta aaaaaagaac ttggagaaac aac
//
LOCUS       DAFEOM010000033        23571 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.034, whole genome shotgun sequence.
ACCESSION   DAFEOM010000033
VERSION     DAFEOM010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.034"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="LP524_001555"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="LP524_001555"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBL2234614.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="LP524_001556"
     CDS             complement(1172..1867)
                     /locus_tag="LP524_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBL2234615.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="LP524_001557"
     CDS             complement(1880..2596)
                     /locus_tag="LP524_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HBL2234616.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3531
                     /locus_tag="LP524_001558"
     CDS             2926..3531
                     /locus_tag="LP524_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HBL2234617.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3856..4179
                     /locus_tag="LP524_001559"
     CDS             3856..4179
                     /locus_tag="LP524_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBL2234618.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4361..5731
                     /locus_tag="LP524_001560"
     CDS             4361..5731
                     /locus_tag="LP524_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBL2234619.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5861..7162)
                     /locus_tag="LP524_001561"
     CDS             complement(5861..7162)
                     /locus_tag="LP524_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBL2234620.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7392..9350)
                     /locus_tag="LP524_001562"
     CDS             complement(7392..9350)
                     /locus_tag="LP524_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HBL2234621.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9527..9793)
                     /locus_tag="LP524_001563"
     CDS             complement(9527..9793)
                     /locus_tag="LP524_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBL2234622.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10303..12429)
                     /gene="pnp"
                     /locus_tag="LP524_001564"
     CDS             complement(10303..12429)
                     /gene="pnp"
                     /locus_tag="LP524_001564"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBL2234623.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12617..12886)
                     /gene="rpsO"
                     /locus_tag="LP524_001565"
     CDS             complement(12617..12886)
                     /gene="rpsO"
                     /locus_tag="LP524_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBL2234624.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13137..13700
                     /locus_tag="LP524_001566"
     CDS             13137..13700
                     /locus_tag="LP524_001566"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBL2234625.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13953..14474)
                     /locus_tag="LP524_001567"
     CDS             complement(13953..14474)
                     /locus_tag="LP524_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBL2234626.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14477..15319)
                     /locus_tag="LP524_001568"
     CDS             complement(14477..15319)
                     /locus_tag="LP524_001568"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HBL2234627.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15312..16037)
                     /locus_tag="LP524_001569"
     CDS             complement(15312..16037)
                     /locus_tag="LP524_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBL2234628.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16213..16509
                     /locus_tag="LP524_001570"
     CDS             16213..16509
                     /locus_tag="LP524_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234629.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17077..18669
                     /locus_tag="LP524_001571"
     CDS             17077..18669
                     /locus_tag="LP524_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBL2234630.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18682..19422
                     /locus_tag="LP524_001572"
     CDS             18682..19422
                     /locus_tag="LP524_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBL2234631.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19590..20939
                     /locus_tag="LP524_001573"
     CDS             19590..20939
                     /locus_tag="LP524_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL2234632.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21067..21657)
                     /locus_tag="LP524_001574"
     CDS             complement(21067..21657)
                     /locus_tag="LP524_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBL2234633.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21804..23156
                     /gene="brnQ"
                     /locus_tag="LP524_001575"
     CDS             21804..23156
                     /gene="brnQ"
                     /locus_tag="LP524_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBL2234634.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6750 a         4746 c         4490 g         7585 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 agcacaggtg aaggtggcga accagaagtg atcggttcaa cagaagaata atactgaaac
     3541 aatcaggtgg tgttagaagc ggcgggtcag cagcttttaa cacgcctttt ttatttctaa
     3601 tcgtttattt tcatatgggg cttccacttt tgtaaatggc tttacaaacg aaaaatccta
     3661 atttaccaag cttttcctcg catactttta tcgtaccttt ataaaaattt atactttgaa
     3721 aaaaagtatt caattattgt tgcatttttc ctgattcttt attagaatga accctcgttg
     3781 aagtaagtat ttctagttta tcctaggatt aattataaaa ataaaacatt cgttgattcg
     3841 aaggaggtta aacagatgtt tgaaagtttt gatagcagcc gcaaccgtta cgcttcagtt
     3901 ggtgtcgtat caagtttgcc agatgaattg attgacagta tatggtttat tattgaccta
     3961 gatcttaagg gagtcattcc attagaaaat atattaacct ttgatttgat cgaaaatcgt
     4021 ggaaaagtga cgatgcattt ttctcaagca ggaagcaatg tggaaatgga tattgatttg
     4081 ccgtttgcct attcgaacca atttcctgct caagtatacg cctatgatga cggtacgaga
     4141 gaaacgatcc ttttaccaag tgagattaga caatattaag gtgatagata aacgtggatg
     4201 tcccttattt tacagaccag gccaagttgt tgattctttg actcaattct tggcttcttt
     4261 ttatggagtt tcataaatta tcattgattt ctaattggta ttctcaggat aaacgctata
     4321 tactactaac taaattttaa agatataagg agtttaaaag atgcgcgatt taacaaaagg
     4381 aagccccgct aaactgattt tgatgttcac tgttccttta ctgatcggaa atgtttttca
     4441 gcagttctac aatatgatcg atatgatcat cgtgggtcag acgctaggta aaaatgcgct
     4501 ggcagcggtt ggtgcaactg gaagcctgac atttttgatc attggctttg cccaaggttt
     4561 aacagctggt ttagccatta ttaccgcaca acgttacggt gcaaaagatt atcggggtct
     4621 gaagaaaagt tttgctgcta gtgttgtcat tagtcttgtc gtcactgtga ttttaactgt
     4681 cctaagtctc ctgtttattc gtccgatgct tcaattgatg caaactcctc ctgaaatact
     4741 tgatcaagca caaacattta tttcaattat tttattaggg atctttgcat cagtcagttt
     4801 caatttgctt tctaatgtca ttcgcgcact tggtgacagc cgcacaccac tattctttct
     4861 gatcattgcg gtcatcatca acgtcgtact tgatttgatc tttattatct attttggcat
     4921 gggtgtggaa ggtgcagcta ttgctacagt aatcgcacaa gtttcttcca gcgtactgtg
     4981 tctagtgtat atcaagaaaa aaatgccttt gttacagtta cgcaaaaaag atttcaaatt
     5041 tgaaaaagaa gagtttgctg tacacctaaa tgctgctttg ccaatggcgt ttcaatcttc
     5101 gatcatcgct gttggtgcta tcgtgttaca agcagcacta aatagtctag ggacagatgt
     5161 cgtagctgct caagccgctg ctggtcgtat cgaccaattt gcaaatcagc cgatgatgtc
     5221 atttggtatt gcaatggcaa ctttcactgc acaaaactat ggtgcgaaag aatatggccg
     5281 aatcttgcaa ggggtacgtc aaactttact gatgagtgtc ggatttagtc tcttagcagg
     5341 tgctatcgtc attttcttcg gtcatttctt tgttcaattg tttgttagcc catcagaaac
     5401 acgtgtattt gaattagcac aaacctactt caatatcaat gggtctcttt actggatctt
     5461 agcagtcctg ttcattttac gctacacact tcaagggctt ggacaaagca agatccctac
     5521 gatcgccggt atgatggaat tgcttatgcg ttcttttgca gctatcgttc ttacaggaat
     5581 gtggggatac ccaggagcag cagcagcttc tcctttagct tggatcggtt ctgttgccgt
     5641 gttgctttac tcttatatgc gttcgatgaa acaattgaaa cacatgggtg aagaggaaca
     5701 tttcacatta gaacatgcag aagcaagata gccttgatta aaagaaatcg ttgcggctaa
     5761 aaatgaaaac ttttattgaa aatatcttct tcgtttaccg aaaacaggtt ttttagataa
     5821 caaaaaggct gtgacaaaaa atgtcacagc cttttttctt ttattctatt tttctttccg
     5881 tggactctac tttgaaataa tcgatatgtg catgctggta ttctaatacc gtgaaacgga
     5941 atgatgcaat ctcaatgatt gttcctgctt ttatttcttt atatcggcta gaagcaaagc
     6001 ccgcaagtgt agtaaaattc gtttcttcaa actctggaat ctctacatga aaataacgct
     6061 ggaaatcatc taatgttagt ttgccggaga tgatataaga gccatctgac tgttccgcta
     6121 tcaatgcttc gtccaaatct gtctcatctc ggatttcccc gacgatttct tctaaaatat
     6181 cttcaatcgt cactaagccg cttgtccctc cgtattcatc ttttaagata gcaatatgct
     6241 ttcccttttt tcgcatgatc gtcaataatt tttttatagg gatcgtttca gttgttacga
     6301 tcggctcttc aatcaactgt tctactgtca gctggtcatc tttataagat tgcttgatca
     6361 aatctggcaa ggtgacataa cctaaaacat tgtcttttga ttctaagata accggatagc
     6421 gagtatgtcc ttgttcaatc gaagctttta tcgcttcttt gacggtttct gaagcttcga
     6481 atacttgcat ggacgtacgg tctacttgta tatcttttgc caaggtatca tcaaattcaa
     6541 acacgttttc caaataatga tattcttctt tggtcaactc tcctttttca taagaattca
     6601 atgcgactaa tgtcagctct tcttgagaca atgtctcttc tccttcagat acgagacgca
     6661 cacctaatag cttccctagc cctgcagctg aatgattcaa tacccaaata aaaggaaaca
     6721 ttactttata aaaataatga agtggtttaa ctacgaacaa gacgaccttc tccgtattga
     6781 cgatactata gctcttagga agcagttcgc caataaccac atcgatatac gtaactaaaa
     6841 taaatcccag cgccacagag attgtactgc taatagccga actaaaaggt agtttaccga
     6901 tcactggttc tagtaaagcc gcgacagatc cttcaccgac ccacccgatg atcaaaccgg
     6961 ttagcgtaat tcctaattga ctagctgata aatagttatc taaatggtgt gtaacatgaa
     7021 gagctagctt ggcgttcttt acattttcct tttctagttg ttccaatctt gatcgtctga
     7081 ttttcactaa agcaaattca cttgctacaa aaaatgaagt aattgcaatc atcaaaaata
     7141 aaataatgaa attgataacc atattctccc gcctttctct ttttctattg tacccttttt
     7201 ctacaaaaag attgaaaata atgcgtattc atatttagga ataaaaaagg agatgcggca
     7261 tttttcagcc acatctcctt atcacttcaa aatcattgcg ttacttgttg ctaaacagct
     7321 ttttcatgac ctctgataaa cggtgttcaa gtagctgaca ttcgacatta gttcaggtca
     7381 aagcgctact ttcacaaccg ctctttcaac catgttttcc gagtatttcc aatgataagc
     7441 ggaacagttt catgttttac ttcgctgtac tctagcccaa accatgtttc tggcgaggta
     7501 gcgatttttt tgattgccag ttttgcctgc atgatttgac gttgccattt tcctagagtt
     7561 gtcacattgg aaactacttc atcaatcagg acaaattgga aatcgccaac ttctcgccct
     7621 ggtgtcagag agtatctttg tggctgtttt ggcaaacggc cttgtttcat caaatcatgg
     7681 atgacttggc gaacatatac gttgatttcc tgttggaccc tgaaacctaa gaacagattc
     7741 acttggacaa taaaatcagt gtccatcata tctacttgat attctttcgt atatggctca
     7801 tccgtcactt ccacatgaac aaaccaatag actcgtgctc ttttaggccg tttatctaaa
     7861 atcgaataga tgaactgtcg gccaacttta tcttcagcca tatcaggaac caagaagacc
     7921 acatttgtct gatagattgg taaagaagta tcatcacgta attctctaag ctgcgggata
     7981 taatctctca aagacacatc ctcggttgac gattcgcgaa taatgttccc ttgttcccaa
     8041 ataaacatca cgactaagat gatcaaacca atcagaacgg ctacaaaacc gcctcggaag
     8101 aatttgacaa cacttgagat aaagaaaacg acttctatcg caccgaagaa aaaggtaact
     8161 aaatatgcaa gccaacgaga atgcccattt tggataagat aaaagtaaag cagtgctgtt
     8221 gtcatcaaca tcgtcacagt gatcgataga ccgtaagcag cttccatatg atgagacgtt
     8281 cggaatgtga caaccactag agaacaggcg atccataaaa tcagattgac tgctgggata
     8341 tacatttgcc cgatactgtt cccaggatac atgatcttta accgaggcag cagtttcaac
     8401 ttgattgctt ctgaaactag cgtaaacgaa cctgtaatca atgcttgcga agcaatgact
     8461 gcagcgactg ttgcaaaagc aacgccgatc acgatccaac tttgaggcat catttggaag
     8521 aatggattca ggttcttgat ttgctgtgct tcagggtttt gataaacatt caacaaccac
     8581 gcagcctgcc caaaataatt gataaccaaa caaattttga cgtaaggcca gctcagacga
     8641 atatttcgtt tgccgacatg tccaagatcg gaataaagcg cctctgcacc tgtcgtagcc
     8701 aggaaaatat ttccaagaat gaacaagcct aatttgttat ctgcactaaa aagcagctgg
     8761 atagcataat aaggattcag tgcacggata actgtccagt cttgactgaa gttcattaat
     8821 cccattacgc ctaaaaaagt aaaccaagcg aacatgattg gtccgaatgc ttttccgaca
     8881 atatctgtac caaagcgttg gaccatgaat aaaacaaaaa taataactaa agtgataatg
     8941 acgatcacgt tctggttatt accgaaccgg tcaaaaaact gcggaattcc tcgcaagcct
     9001 tcaatcgcag tcgttaccgt cacagcaggg gtcaataccc catctgctag taaggcagct
     9061 cctccgatca ttgcaggaat gatcaaatat ttcccacctt ttcggacaag tgtgtagagt
     9121 gagaaaattc caccttcacc atggttatct gcattcaatg caatcacgac atatttgatt
     9181 gttgtcagta gagtcaatgt ccaaaaaatc aatgagactg atccgataac aaaatcagga
     9241 ttgattcctg acaatccgcc attatcttca acaatcgatt tcataacgta aagcggactt
     9301 gtcccgatgt ctccgtagac gactcccatc gctacaagca ggccagccat tgatatcttt
     9361 ttggtattgt gcgttgttgc cacgtttttc cctcatttct tgttatatgg atatccttta
     9421 ggtaagcttg aatgatagat gatattctta tccatgacac ccgctttcct aaattgcttc
     9481 taattatcca agtaacacac tgaagtgtca acttatttga ataaaattat tctttctttg
     9541 gacgttctct gatacgtctt gccacaaatg aataaagagc aaggcaaaaa attgcaccca
     9601 ataatgctgg tatcaaataa aaaccgccga gttccggtcc cagtggtcct agtaaaaatt
     9661 cgccgagcca actacctaaa aaaccgataa tgatatttcc agatacgcct cccgggactt
     9721 cctttcctaa aattgcgcca gctatcaaac ctaatatacc gccgataaat aacgaccata
     9781 aaaaattcag catctccttt ccctccttta tccatatttc tcacttttat tttacgcttt
     9841 attcagaagg actccaaaaa tatgcgccaa gaaactccag aaaaaaagaa gttgtaacaa
     9901 aagtgttcag cttatttccg tagaagctgc ttctggaaca ccgtgaatca gaggttgtga
     9961 aatcagcggt ttgacctgag tattagagaa acaaacggaa aaatagttcc tcatattttg
    10021 ccgttttttg agctaatatc gagggacagc tgattgaaca tcgtttattg gagaggttgt
    10081 aaaaaagctg ttctgcatca agtaataaga acagccaagt aaaaacagct cctaatgttt
    10141 ttaacttggc tggacttatt accgcagatg caagcttttg aacaccgttt attcgaaaaa
    10201 aaaggactgt gacacggctt ttgtcacagt cccctttttt cgaataatta ttgtccccct
    10261 gtcaccttca cgatcacaag tacattattc ctatgaatta aattattttt cttcattttt
    10321 cgttgaacca tcttcgttca acattgcttt tcttgacaga tttacgcgtc cttgtttatc
    10381 gatttcagtt actttcacaa ggatctcatc acctagttta acaacatctt cgactttatt
    10441 gacacgatcg tttgctaatt gggaaatatg gacaagtcca tcttttcctt tgatcaagtt
    10501 gacgaatgca ccgaattttt cgatacggac aactttccct agataaactt caccaacttt
    10561 gacttctttg gtcaaatctt cgatgatctt gatagctttt ttgatcattt ctgcatcagc
    10621 tgaagcaata cttacttttc catcttgatc gatatcgatt ttcacaccag tttcatcgat
    10681 gattccgttg atcgtatcgc cgcctttacc gataacatct ttgattttag caggttcgat
    10741 ctggatcatt tcgatttttg gtgcatattg gcttaattct tcacgtggag cagcaatcgt
    10801 tgaagtcaat tcttctaaga tttccatacg tgcttgtttt gcttgagtta atgcttcagt
    10861 taggatttgt tctgtgattc cttgaatctt gatatccatc tgtaaagcag tgataccatc
    10921 ttttgtaccg gcaactttaa agtccatatc gccaagatga tcttccagcc cttggatatc
    10981 agttaggatc gtatagtttt caccatcaga aacaagtccc atcgcaatac cggcgactgg
    11041 cgctttgatt ggtacacctg catccatcaa tgctaatgtg ccagcacaga tacttgcttg
    11101 ggaagaagaa ccatttgatt ccaatacttc tgctactaaa cggatcgtat aaggaaattc
    11161 ttcttctgaa gggattactt gtgctaacgc acgttcacct aatgcgccat gtccgatttc
    11221 ccgacgccct ggagagcctg cacgtcctgt agaaccaaca gaaaattgag ggaaattgta
    11281 gtggtggatg aatcgtttgc tgacttccac ccctaaacca tcaatgattt ggtgctctcc
    11341 tagaggtgcg agtgtacaag ctgataaagc ttgtgtttgt cctcgtgtga acaaacctga
    11401 tccatgtaca cgtggcaata agctgacctc tgatgacaat ggacggattt catccaattt
    11461 acgcccgtca ggacggatct tatcgatggt gatcaattca cgtacgacat ctttttcaag
    11521 atcttctgtg atttgtttaa cttctttcaa caagtgatct tcatcttcat gacctgcaaa
    11581 tttctcagca taagcttcgc ggatatcgat cttcacctgc tcgatattat cttcgcgagc
    11641 aagtttttct tcagtcatta ctgctgcttt cattgtagcg taagaagcat caaagatttc
    11701 ttttttcaat acttcatcaa cttgcagtaa agtcacttcc attttttctt tccctactgc
    11761 ttgaacgatt tcttcttgga aagcaactaa ctctttgata gcgtcaaaac caaacagcaa
    11821 tgcaccaagc atgtcttctt ctgaaacttc tttcgcgccg ctttcaacca tattgatggc
    11881 ttgtttcgtt ccggcaacag tcaattcgat atctgtttgt tctgcttgtt ctactgttgg
    11941 attcaagacg tactcgccat ttacacgacc aacttctacg cctgcgattg ggccgtcaaa
    12001 tgggatatct gagattgata gtgctaaaga agaacctagc attgctgcca tagctggcga
    12061 gcagtctgtt tctacactca tcacgatgtt cgttacttgg acttcattac ggaagccttc
    12121 tgcaaacatc gggcgaattg gacggtcgat caaacgggca gtcaatgttg cttccgtact
    12181 tggacggcct tctcgtttga tgaaacctcc agggattttc cctgctgcat acattttttc
    12241 ttcataattg atcgttaatg ggaaaaagtc tgtatctttt gcttctttag aggccacagc
    12301 tgcacttaag actactgtat ctccataacg gactaaaacg gctccgtttg cttgttttgc
    12361 tagctgaccg acttcgattt gtaagggacg gccgccccat gtcgttttga atacttgttt
    12421 ttctgacata acttctcctt ttccttctcg tgaaagcaag acgctactaa acaaatgaaa
    12481 actgccgaaa gccgtctttt gagcagcttt catttggtta gtaggagtcc atctagcttg
    12541 atcacggaat tttacttaat tgatatacga gtaaaaaaag tgagattcat gtgaatccca
    12601 ctctttttga ctaaaattaa cgacgcaagc ctaaacgttt gatcaattcg cggtaacgtt
    12661 gaacgtcagt tttacgtaag taagctaaca agttacggcg gtgaccaact tttttcatta
    12721 ggccgcggta tgaatgatgg tcttttttgt gcacgcgtgc atgttcattc aagtggttga
    12781 tttcttcagt taatacagcg atttgtactt ctggagaacc agtatcccct tcatggcgag
    12841 cgtattcgtt gatgatttca ttcttgcgtt cttttgaaat tgccatttct ttcacctctt
    12901 tcgattattc ccctgcgact gagtaaaacg ttggtgattc gcttaaccaa gtggcaggtc
    12961 atgtgcctag agcacacagc tatactttac ctgaaaccgg gttgaaaatc aagtagttaa
    13021 tcattaagaa aaggtagttt tcaaacaatc ttactactac ttttccattt ttgtggtaaa
    13081 ctataatgcg aaaatagact tgaaacacag tcttttaaga aaggggtaca tattccatga
    13141 ttacaatgga tgatatcatt cgtgaaggaa atccgacctt acgcgaagtt gcaaaagaag
    13201 tttctcttcc attaagcgaa gaagatatct ctttaggaaa agagatgctt gagttcttaa
    13261 aaaacagcca agatccaatc aaagctgaag aactacactt acgaggcggt gttggcttag
    13321 ctgctcctca attagatatt tctaaaagaa tcattgctgt tcacgtacca agtcctgacc
    13381 cagaggcaga tggaccttct ctaagtactg ttatgtataa tcctaaaatt ttgagccatt
    13441 ctgttcaaga tgcttgttta ggggaaggtg aaggctgttt atctgttgat cgagaagtac
    13501 caggatatgt tgttcgccat gccaaaatca ctgtgtctta ttatgatatg aatggtgaaa
    13561 aacacaagat tcgtttgaag aattatgaat cgatcgtcgt tcaacatgaa atcgaccata
    13621 tcaacggtgt gatgttctat gaccacatca acgatcaaaa tccatttgca ctaaaagaag
    13681 gcgtattggt gatcgagtag agaattggca ggttccttcg atactatgct acaagaaagc
    13741 tttataaaaa aggggagtgc tcaaaagctg cacttccctt ttttatgttt ctccactaat
    13801 cgaaatttag atagacatct agaaatcgca attccaaaaa attctgtgta ttggatacgc
    13861 atcttttctt attttttctt atttcttcca tatgggtgtt tatctcagag atgatagaat
    13921 agtttctagg ataaaaagta gcagctacga tgttatcctt cgtagaggat tcctgcttca
    13981 gctagttttt gtttgatgtt tttcagcata tgcttttctg ggtagcggat cgtatgcaga
    14041 tggatgccgc ctgttaattc ggataacaaa gtagcattac ttttttgata acgtctcata
    14101 aaggaatcga tatcttgtgg cgttttgatg tgcaaagttc ctgctaactc accgtaaagg
    14161 ggatgttcca caattacatc aatgatttca ccaccatttt caacgattag tctcagttct
    14221 tcttctgttt gatcttttcc atgctggaca gcgattttac tgatgtgccc agtattggtt
    14281 tcttctgttt cgaggatata acctcttgct gttgccacga tacttttacc tgcagctcgt
    14341 agtaaagcaa tatcgcctac aatgatctga cggctgactc caaaagtttt cgcaaacttg
    14401 ctagcactca ctggtttttc tgtatttgcc aaggtttgca tgatttgttt tcgccgtgtc
    14461 tctccttcga tcacacttat tcctccttca ttttgttttg gtattcgcgc gagtgtttca
    14521 tcaaatcaac atacgtgtct gttattgttt ccttatatcc cttatttttc agcagactaa
    14581 caactttatc caatacttgc tgctctcttt ttgcatcata aactgcttgg ttttcttctt
    14641 ttttcacttg tcctatttta gttaccgtat ccattctttt ttctaataaa gcaaccaact
    14701 ctcgatcgat catatcgatc ttatgacgta atgtttcaag agactcttct gttagttctt
    14761 cctctactgt cttgatgata cgagcaggat ttcctgcaat cactacattg tcgggaaaag
    14821 atttggtcac aaccgaacct gcgccgacta ctacgttgtc ccctagagtg acacccggcg
    14881 tgacgataac tcctccgcct aaccaaacat tattaccgat tttaatgggt tttgcgtact
    14941 ccagaccgct gttacgtttc actggatgta gtggatgcgt cgccgtgtaa agctggacat
    15001 tcggagcgaa catgcagtta tcaccgattt caatcgtgct gacatctaaa aatgtacaat
    15061 tgaaatttgc ataaaaattc tccccaacaa atatattata tccgtaatca aaattgataa
    15121 ctggctccat atagattttt ttccctgtac ggccaaaagt ctctttcaac aattgggatc
    15181 gcagttccgc tgattctgct tggttgatga tttggctttg acttcttgca aacatacggt
    15241 cggcaaccag ctcaggatca ttggcaaaat aaagctcgcc tgcaatcatt ttttcttttt
    15301 cggttttgaa ttcagtcata atctgctcct aattcttgta tttttttgaa aacaaccgct
    15361 ccgtcttctt gaataaacgt ataaggctcc gctaaataaa caggcgtttg attgattact
    15421 aagattttgg ctgacggctg gcgaaactgt accagatcac aaaacggatg aacttgaaaa
    15481 ctcgttccaa caatgacgat cagctctgct tggctgacgg cataagccgc ctgctccatg
    15541 actccatctt gaaacccttc accatataac acgatgtctg ggcgtatttg tcctccgcat
    15601 gcttgatgtt tgtcgctgtg aagatattct ttccagtcta ccgtttgttg acattttcga
    15661 caatgacatt gatacagact cccatgaaaa ttgacgagtt gcgggctttt agcctttgcg
    15721 tgcaaaccgt cgatgttttg ggaaacaacc caaacatttt tttctttcgc tagatgtgct
    15781 atttccaaat gaatgatatt gggttttgct tcaggatgat aaatcttttt gacaaattga
    15841 tagaatttat ccggttcatt caccattgct tgatgactca gcaagtattc cggctgctcc
    15901 atcccgtggt agattcctga caaagatcga tagtctggca ctccagaagg tgtggatacc
    15961 cctgcaccgg tcaaaaatgt aatttgctga taagctaata tctcttttgc tgcttcgtct
    16021 actgttaatt gtttcatgtt ttgtcacctc actttccatt atagcccata ctcgaacaaa
    16081 tttattgttg aaaagtcaga aaaatgggaa taaaaaagtg ttatgggcat ttgagcaatt
    16141 tatcacaatg ttcactgaca aaagaatgaa aacgtgttac aatactgctg tataaaagtg
    16201 aggtgaatcg atatgttacc attctttatt tattggggcg ttattctggc ttgtatcgcc
    16261 tggttagcgt taagcgttta tttctctgtt ttttatcttg tacgcaaaga aaatggaaat
    16321 ctttgggctt ttgctttatt caacgtgatt gccgcaattg ttttagccat tacattagcc
    16381 gtatatcgaa catggggctg gggaatcacc caatactcaa gtttgattta tctaatatta
    16441 gccatttatg gcgtcgttgt tcttttgcaa gcgatcctag gacgtgagcc aaagaaagcg
    16501 gcagcttaaa tgatcgagat ggaactggac acaggaaatt ttctgttcta gttctttttt
    16561 tattgtatta catacatttt tgtgtataat tatactatcg tttgcaatgg atagatgatt
    16621 cacattttga ttgttttttc ggtaaaagta aagatttcta tatttatcag tattttttca
    16681 aaaaccagca gttatagatt gacaaagcct ctcgagtatc gtaaactttt tttgtataaa
    16741 taactgaata cgagatgcct caatcaatga ggtagaggtc gcgagaatca ttaaccctgt
    16801 ggagtgaagc aaacatggag aagcaggaat agggaggatc gccgaaatgt gcactttttt
    16861 gcttggagtg tatgttggga cgcaagttaa gagcttgcgg actgtcttag cagtgatgct
    16921 aagttgcgct atgttttgtg agaggtttat caccttatgg aattttagcg gcctttttat
    16981 atctataaat cggtcgcttt ttattttttc agataaagct ttaccgcatt tcatctagac
    17041 cgctcgtgta tcatataatc agaaagggag aaaaaaatga aaaaaaatat ggtttcagtt
    17101 gccgtactat ttgcttttgt cttctcacta ttcagtgtgg gatctctggc aaatgcagat
    17161 gaaaaaacac cgagcgatga gtttcgcgta ggtatggagg ctgggtatgc acccttcaac
    17221 tggtctcaac aaacagatga gaataatgcc ttgccaattc aaggccaaaa tagttacgcc
    17281 ggcggttatg atgttcaaat cgctaaaaaa gtagcagatg gtttagggaa aaaattagtc
    17341 atcgtccaaa cgaaatggga tggtctggct cctgcccttc aatcaggcaa gatcgacgcg
    17401 atcatcgccg gaatgagccc tactgctgaa cgacgaaaag aaattgattt taccgatcct
    17461 tattacgaat cacaattagt agtagtagtt caaaaagata gcaaatatgc aaaagcaaaa
    17521 agtttagcag atctttctgg agcaaagatc actgctcagt taaatacatt ccactatact
    17581 gtgatcgatc agataccaga tgtgcagaaa caacaagcaa tggataattt ctcagcaatg
    17641 agaacagctt tagcttctgg aatgatcgat ggttatgtca gcgaacgtcc ggaaggtgta
    17701 acggcaacta gtgtgaacaa agacttggaa atgcttgaat tcagcggtgc agatggcttc
    17761 cagacaaacc cggaagatgt tcagatttct gttggtatgc gtaaaggcga cccggatatc
    17821 caaaaagtaa accagattct ttctggcatc tctaacgatg aacgaaccaa aattatggac
    17881 caagcagtca aagatcagcc tgctgcaact gatagtgaag aaaaacctgg catcatttct
    17941 gatttcacta cgatttggaa acaatacggc aatatgttct tacgaggtgc tggacttacc
    18001 ttgttcatcg cattgatcgg gacagttgtc ggtactacac ttggattact gattggtgtc
    18061 ttccgttcgg tacctgaatc aggaaatgca ctgactcgct tcttccaaaa aatcggaaat
    18121 gcacttcttt ctatctatgt cgaagtattc cgcggaacac cgatgatggt acaagcaatg
    18181 gtcatcttct acggtctggc actcgctttt ggcatttcat tgaaccgaac gattgccgct
    18241 ttgtttattg tctctctcaa tactggagct tacatgacag agatcgttcg tggggggatt
    18301 tttgcagtag atgaagggca gttcgaagct gcgcaagcca ttggtatgac ccatggccaa
    18361 acgatgagaa aagttgttat tccacaagtt ttacggaata tcttgccagc aacaggaaat
    18421 gagtttgtta tcaatatcaa agatacagca gttttaagtg taatcggcgt agctgatttg
    18481 ttcttccaag gaaactctgc ttccggtgcg aatttccaat tcttccaaac atttacgatc
    18541 gtcgggatca tctacttagt catgaccttt acgatcacca gaatcctgcg ggcggtagaa
    18601 aagaaaatcg atggtccttc agcttatacc aagcttgaag aaatcgacaa ttcaaatttg
    18661 caggaataag gaggaagaat catgaatgaa aaaattattg aaatcaaaca tctaagaaaa
    18721 agcttcggtg aaaatgaagt attgaaagat atcagcgtaa cagtcaataa aggtgaagtt
    18781 gtgacgatca tcggttcttc cggttctggt aaatctactt tcttgcgctg tatcaattta
    18841 ctggaaaaac caacaggcgg cgagatcatt tataatggag aaaatgtatt agctcctaaa
    18901 tataatttac ctaaatatcg aacgaatcta ggtatggttt tccaatcctt caacctgttc
    18961 aacaatatga atgtcttgga aaactgtatg tctggtcaaa caactgtcct gaaacgagat
    19021 aaagaaactg ccaaaaaagt agcaatcgaa aatctggaaa aagttggtat ggatcgttat
    19081 atcgaagcca aaccagcaca attatctggt ggacagaaac agcgtgtcgc aattgctcgg
    19141 gcattatcaa tgaatccaga tgtattgcta ttcgacgaac ctacttcggc gttagaccct
    19201 gaaatggttg gcgaagtgct taatacgatc aaagacttag cacatactgg cttgacaatg
    19261 cttatcgtga cgcatgagat ggaatttgca aaagatgtat ctgaccgtgt catttttatg
    19321 gataaaggtg tgattgcaga agaaggtaca ccagaagata tctttgtcca tccgaaagaa
    19381 gaacgtacaa aagaattcct ttcacgtatt ttaaatgctt aggatcaata gctgattggg
    19441 acaaagagct gcggtgttta ccgcagttct tttttatgaa ttactcataa aaggtaaatt
    19501 tctgaaaatt ttggaatctt agttgtgttt ttcagaaaac tctcttaata tggaagtaat
    19561 taaacttttt tagttaggat gatttaaaaa tgaatgaaga gcaagaactg cagcgtggac
    19621 tgaaaaatag acatgtccaa ctgatttcta ttggcggagc gattggaacg gggttgtttt
    19681 taggttccgg aaagtctatc cagctagctg gcccttcgat attgctcgcc tatcttatta
    19741 ctggatgcat ctgttttttc attatgcgtg cgttaggcga acttctgtta tcaaacacca
    19801 ataaccattc ttttctggat tttgttgcag aatatcttgg caaaaaagcg gctttcatca
    19861 ctggctggac gtattggttc tgctgggtat cgattgcaat ggccgattta acagccatcg
    19921 gaatgtatgt acgctactgg gcgcctggtg ttcctcagtg gcttcctgaa ctgattgcat
    19981 taggtcttct tctttgcttg aatatggtgg ctgtctcatt gtttggtgag ttagagtttt
    20041 ggtttgcatt gatcaaagtt gtagcgatca ttgcctttat cctcgtagga gcttatatga
    20101 tcctcaccca ctatacgaca gatattggca cagccagtat cacgaatcta tggtctcatg
    20161 gcggattctt tccaactggt gcaaaaggat ttatcttagc tttccaaatg gtaacctttg
    20221 cttttgctgg gatagaatta gttggattag tcgctggaga aacagaaaat cctgaaaaag
    20281 tgttgccgga agctatcaat aatattccta tcaggatcat cttattttac ttaggatctc
    20341 tttttgtcat tatggcaatc tatccatgga acagcttgaa tccagataac agtccatttg
    20401 tagaagtatt ttcggaaatt gggatcacga ttgctgcttc cttgatcaat ttagtcgtat
    20461 taagtgcagc agcttctgcc tgcaacagtg caatatatag tacaggacgt atgcttcgtt
    20521 ctttagcgca agaaggcagt gcccctaaaa aattcaaaaa gttaacaaca catcatgtgc
    20581 cagggaatgc tttgacattt tctactatcg tcattttcat ttctgttatc ttgaattacg
    20641 tgatgcctag tgaagtattc acactagtaa gcagtattgc cactacttgt ttcttgttta
    20701 tctggagtat gcttgtctac acgcacatga aatatcgtaa aagtatcctc ggcaaaaaag
    20761 ctcattcatt taaaatgccg ttgtacccat ttagtaatta tctggtattt ctttacatgg
    20821 cctttgtgtg tgtcgtcttg ttcctaggaa aagatacgag aatcgcttta ttactaacac
    20881 ctgtgtggtt cttgcttcta ttactaatct atcatatgaa atatgaacgt aagaagtaag
    20941 aaagtagttg tgacagaagt ggtcagcttc gagaaataag aaggaattta cgaaaattgc
    21001 ttcttaaatt tttgtgaaat ttcggcttat ttcttggaga taaaagcttc tgtcccagcc
    21061 tcttttctat ttccccggaa acaaccgctg ataataggca tctgcttttt tataggaaaa
    21121 aatccaaata ccagatacaa aaaaacgtcc gaataacccg atcaacggta caaaaaagac
    21181 accggcaatc aagcataagt taatcatcat ggagataaga cttcgacggt cctttgacaa
    21241 aactctcacg acatgggaat ctggtttatt tgctttcatg atttgcctca tcaagatacc
    21301 aaaagaaaaa ctccacatcg tgtatactaa gacataagcc atctcaggcc aaaaagcttc
    21361 agggttacgc ccgacccaac tagtaacaga aggacataaa gtcagccaaa agagcaaaaa
    21421 gatattcgac cagaccactc ggtaattaat tcgttctgcc atctgaaata tacgatgatg
    21481 gttcacccag atcaccgcaa caaatataaa gctgatggta taagctaata atgtatttcg
    21541 gatgacccat aaagaatgaa agctagatgt attagggatc ttgatctcta atacaataac
    21601 tctcaaaaca atggctacca ctccatcagt aaatgcttct aatctcgact ttgtcacgat
    21661 gattcctcct cagatgattc agcgctttta ttttatcact tacgttatta aataaccatc
    21721 gttttatata gggaattttc tgaaaattgt tgcatatttt tcgaaatttt gttactttta
    21781 aaaaaaccgt aaaggagtta tgtatgacaa aaaaattatc atttaaagac tatcttttta
    21841 taggctccat gcttttcggt ctattttttg gtgctggaaa tcttatcttt cccgttcatt
    21901 tgggtcaaga agcaggcgct gctacctttt gggcgaatct aggtttcttg gttaccggta
    21961 tcggtcttcc cttcttaggg gtgattgcca ttggtgtatc gaaaagctca ggtgtatatg
    22021 aattagcaaa acggataaat caaacatatg cgatggtttt taccatcttg ctttacttag
    22081 ttatcggacc gttttttgct ttacctcgcc ttgcaaccac atctttcgag attggtttag
    22141 caccatttat cgatcctagc agacagacgc tatatctagc aattttcagt attgtatttt
    22201 ttgtgctggc ttggtggttt tcaagaaaac cgacaaagat attagattat gttggaaagt
    22261 tcctgaatcc tgcatttttg gttttattag gtgctttgct tctccttgct tttttgaatc
    22321 cactaggttc tgtaaatcaa gcgcctattc agccaaatta tcaagagcat gcttttttca
    22381 cagggtttac tcaaggatat aacacattag atgcgcttgc tgctttagca tttgggatca
    22441 tcattgtcac aacaatccaa aacatgggtg taacaaaacc tgctgaaatc gctaaagaca
    22501 cgatcaaatc tggtgcgatc agcattatat tgatgggaat catttatact ttgctcgctt
    22561 atttaggtgc tatgagctta ggtagctttg ctctaagtga aaatggcggg atcacgctgg
    22621 ctcagattgc tgatcattat ttaggtactt atggcagtat cttattagca tttatcgtta
    22681 tattagcttg cttgaagacc ggtattggtt tgattacagc ttttgcagaa acattcacag
    22741 atttgtttcc gcaaagaaat tacgcattct ttgttgcact tgctagtatc cttccttgtt
    22801 tagcggcaaa cgtaggcttg acgaatatca ttcaattttc gttgcctgtt ttgatgttca
    22861 tctatccatt agcaatgaca ctgattttac tagtgttagt tggtcctttg tttaagcagc
    22921 gtacggctgt ttatcgaatg actacttatt ttacattgat tgcttcgatt tttgatgggt
    22981 tgaacgcttg cccagaaact atcaaacaaa cacctattgt tcaaaatctt ttatatgttg
    23041 cggaaagcta cttgccgttt ttcaaattgg ggatggggtg gatcgtacca gcagttatcg
    23101 gttttgttat cggactgatt tggagtttcg ctaagaaaga agaagtagca gattgataaa
    23161 agaggttgtg aaagagacgt tcagccttga gcaataaaaa gtaattttga aaaatggctt
    23221 ctcacatttt tgcataaatt tgacttaata ccgagaaact gacttttgaa caccgtttat
    23281 tgagaggtcg tgaaaaagcc gcttagtttc aagtattaag aaatattttg tgaaaatggc
    23341 ttctcacatt tttgcataaa tttgacttaa taccgagaaa ctggcttttg aacaccgttt
    23401 attgagcgtc tataaactga cctgagtatt agagaaacaa aacggcaaag tagctccata
    23461 tactttgccg ttttgttttg aactaatacc gaagagaagc tgatcaaaca acatttcttt
    23521 tataatatag attttagaca tatatatgat ataaaaatag ttttcgcaaa a
//
LOCUS       DAFEOM010000034        23145 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.035, whole genome shotgun sequence.
ACCESSION   DAFEOM010000034
VERSION     DAFEOM010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.035"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     misc_feature    111..236
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            280..804
                     /gene="infC"
                     /locus_tag="LP524_001576"
     CDS             280..804
                     /gene="infC"
                     /locus_tag="LP524_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBL2234635.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            876..1076
                     /gene="rpmI"
                     /locus_tag="LP524_001577"
     CDS             876..1076
                     /gene="rpmI"
                     /locus_tag="LP524_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBL2234636.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            1129..1488
                     /gene="rplT"
                     /locus_tag="LP524_001578"
     CDS             1129..1488
                     /gene="rplT"
                     /locus_tag="LP524_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBL2234637.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            1740..3047
                     /locus_tag="LP524_001579"
     CDS             1740..3047
                     /locus_tag="LP524_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HBL2234638.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAIFA"
     gene            3124..5253
                     /locus_tag="LP524_001580"
     CDS             3124..5253
                     /locus_tag="LP524_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBL2234639.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKQQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            5228..5917
                     /locus_tag="LP524_001581"
     CDS             5228..5917
                     /locus_tag="LP524_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234640.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(6325..7011)
                     /locus_tag="LP524_001582"
     CDS             complement(6325..7011)
                     /locus_tag="LP524_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL2234641.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(7147..7341)
                     /locus_tag="LP524_001583"
     CDS             complement(7147..7341)
                     /locus_tag="LP524_001583"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234642.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(7391..7831)
                     /locus_tag="LP524_001584"
     CDS             complement(7391..7831)
                     /locus_tag="LP524_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HBL2234643.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(7835..8680)
                     /locus_tag="LP524_001585"
     CDS             complement(7835..8680)
                     /locus_tag="LP524_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HBL2234644.1"
                     /translation="MLINEQLVKRIITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            8829..10247
                     /gene="pepV"
                     /locus_tag="LP524_001586"
     CDS             8829..10247
                     /gene="pepV"
                     /locus_tag="LP524_001586"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBL2234645.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(10304..11251)
                     /locus_tag="LP524_001587"
     CDS             complement(10304..11251)
                     /locus_tag="LP524_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL2234646.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(11474..11824)
                     /locus_tag="LP524_001588"
     CDS             complement(11474..11824)
                     /locus_tag="LP524_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBL2234647.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            12024..13103
                     /gene="pepA"
                     /locus_tag="LP524_001589"
     CDS             12024..13103
                     /gene="pepA"
                     /locus_tag="LP524_001589"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HBL2234648.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            13247..13567
                     /locus_tag="LP524_001590"
     CDS             13247..13567
                     /locus_tag="LP524_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBL2234649.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            13589..14053
                     /locus_tag="LP524_001591"
     CDS             13589..14053
                     /locus_tag="LP524_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBL2234650.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            14258..14863
                     /locus_tag="LP524_001592"
     CDS             14258..14863
                     /locus_tag="LP524_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HBL2234651.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(14902..16191)
                     /locus_tag="LP524_001593"
     CDS             complement(14902..16191)
                     /locus_tag="LP524_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBL2234652.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENIKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            16619..17098
                     /gene="rlmH"
                     /locus_tag="LP524_001594"
     CDS             16619..17098
                     /gene="rlmH"
                     /locus_tag="LP524_001594"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBL2234653.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            17183..17692
                     /locus_tag="LP524_001595"
     CDS             17183..17692
                     /locus_tag="LP524_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HBL2234654.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            17792..18442
                     /locus_tag="LP524_001596"
     CDS             17792..18442
                     /locus_tag="LP524_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234655.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            18893..19831
                     /locus_tag="LP524_001597"
     CDS             18893..19831
                     /locus_tag="LP524_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBL2234656.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            19844..20896
                     /locus_tag="LP524_001598"
     CDS             19844..20896
                     /locus_tag="LP524_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL2234657.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            21162..21797
                     /locus_tag="LP524_001599"
     CDS             21162..21797
                     /locus_tag="LP524_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HBL2234658.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYRLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            21988..22779
                     /gene="msrA"
                     /locus_tag="LP524_001600"
     CDS             21988..22779
                     /gene="msrA"
                     /locus_tag="LP524_001600"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBL2234659.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
BASE COUNT         7377 a         4068 c         4789 g         6911 t
ORIGIN      
        1 ggaatcactg ctagaaagtc tgctaatcta atcattttca gaaaaggaag acaaactgta
       61 tgaaaaacat tgacaaaaag attagttttt gctatactat gatggttgag aaataaaagc
      121 agaagcaccc gcttctcgcc ttagttgata atgtcactgg gctagatagt tttcactggt
      181 tctattaggt gaagtacgcg cgggttctgt aagagaactc gtttttttat tgccgtttat
      241 tcggtaagct tttctttctc tcataatatt tggaggtgaa tgaccatagc aaaggatatg
      301 atggttaacg acggcattcg tgcacgagag ttacgattga tcggttcaga tggtgagcaa
      361 ttaggagtta agacaaaagc agaagcattg aatattgcag aacaagcaaa cttggatctt
      421 gtgttagttg cccctggtgc gaaaccacca gttgcgcgaa tcatggacta cggtaaatac
      481 cgtttcgaac aacaaaagaa agaccgcgaa gcgcgtaaaa aacaaaaagt gatcaatgta
      541 aaagaagttc gcttaagtcc tacgatcgac ttgaatgact tcaatacaaa acttcgtaat
      601 gcacgtaagt tcttggaaaa gggcgataaa gtgaaagctt ctatccgttt caaaggccgt
      661 gccattaccc ataaagaaat tggtcaaaaa gtcttgaatc gcttagctga agaaactgcg
      721 gatattgcaa cagtggaaca aaaagcgaaa atggacggac gcagcatgtt cttgacgctg
      781 gcaccgaaaa acgacagtaa gtaatttgaa tgactaactg gctgttggac agccgatagt
      841 cggataacga aagattttga ggaggaaata tagtcatgcc aaaacaaaaa acacaccgcg
      901 gatcagcaaa acgtttcaaa cgtactggta aaggcggatt gaaacgcttc cgcgcgttta
      961 caagtcaccg tttccacggc aaaacaaaaa aacaacgccg tcaattgcgt aaagcaagca
     1021 tggtttctag tggcgacttc aaacgtattc gccaacaact agcaaaaatg aaataagaag
     1081 tacaaataac agaatctgaa gatttatttt tattaggagg aattatacat ggcacgtgtt
     1141 aaaggtggaa cagtaactcg taaacgtcgt aaaagagtgc taaaattagc aaaaggttac
     1201 tacggttcaa aacacacttt atttaaaaca gcaaaagaac aagtaatgaa ttcatacaac
     1261 tacgcatatc gcgatcgtcg tcaaaagaaa cgcgacttcc gtaaattgtg gattgctcgt
     1321 atcaacgcag cagctcgtat gaatggcttg agctactcaa aattaatgca cggtttgaaa
     1381 gttgctgaaa tcgacatcaa ccgtaaaatg ttagctgact tggctgttaa cgatgcagca
     1441 gcatttactg cattggctga ccaagcaaaa gaagctttgg ctaaataagc taaacaatta
     1501 taaagaggaa agaagataac gaaaagttat gttctttcct ctttttttat ttacaatcct
     1561 ccatcaaatg aaacaggaca ataacgtata agtctaaaat agcataatta aggtttttta
     1621 attgaaaact gaacatttat ggtcaccctt taatgaaagc gctaactaca taagttagaa
     1681 tgagtttgac tgatatatta gtttgcttat cagaaaaaca aaatggacgg aggaataaca
     1741 tgagtgatat tattattggt attttgttag ttatctcatt ctttttcatg gtctggtact
     1801 gtgtaaaagg ctacaacttg atggtcggct ttgccatcat ggctactgta tggatgggat
     1861 tagcgctagt cggaaacacg ttttcaccaa acccggcgat ggaagggcaa ggagtaattg
     1921 atgtgttgac gcatatctat acgacggggc cagctgagta cgccaaatcg atcttggtaa
     1981 atgtcttctt tggtgccttt ttcgggcgtg ttctagtaga tagcggaatc gcagctaccc
     2041 tgattcgaaa ggtagttgaa cttggagggg acaagccaag aatcacgatg agcttactat
     2101 gtattgtaac cgctgttata ttcatgtcga tgacaggaat cggaccagtg atttctattg
     2161 cagttattgt gttgccaatc ttgatgtcat taggtatttc agtaccggtt gcactgttct
     2221 catttatggg ctcgattatg gctggaatct ttgctaatat cgtcaatttc aaacaatatc
     2281 aaacgattta tgctggtttc aatccagcgg ctgaaagcta tacttataat gactatttcc
     2341 aaattggtat gattgggatg attgtaagct tagttgtggt actaacggtc gctaatatat
     2401 caatgaacaa aaagaaacgc tatgcaatgg cagcaaacgt accagctgaa ggtggcgacg
     2461 caccaatgat ctcatggtta gccgtattat taccagtttt aggggtcgtt cttttagatc
     2521 ttccaatcat cttaggtttt attctagcag gtatctgggc attgctgttt actggaaaat
     2581 tgcgcggagg ttataaagaa atctgtcgtc agtttgcgaa attatttaca gatggcgcag
     2641 tagatgttgc accaatggtt ggtttcttga tgaccttagc gatgttcaat aattcagctg
     2701 cgtatgcttc tccttatttt tcagctattt ttggagattg gataccgcaa tcaccattag
     2761 tcttggcaat cgtatttgct atattgacac cactaggatt tttccgcggt ccaatgaatc
     2821 tagtcggttc aggctcagct attttagctg tcgtattagc tgttaatcca acgatgtcgc
     2881 cagccttttt gttcccgtta tttgcaatca caacgattgc tcctcagcat ttagatatta
     2941 cacaatcttg ggttgcttgg ggattaggtt atacaaaagt aacttctcga gaatatatga
     3001 agaaatccat cccaactggt tggatcatcg gagcgatctt tgcctgatca cgttcttatt
     3061 gtacgggaac gcctagtaaa caaaacgtcg ataaaaacga ttagatgaat ggaggaaaag
     3121 aaaatgaaac gttttgtgcg aatgggaatt gatgttggcg gtacacatac gaaagcagtc
     3181 gcaatcgata atgcgacgca cgaaatcatc ggaaaatctt cagtgaaaac aacccacgat
     3241 gatgtccgtg gagtagccgc aggtgtcgtt cagtcttttc aaaattgttt gcgggaaaat
     3301 aatatcagtc cagaagatgt tgtgtttgtt gcacattcta ccacacaagc gactaatgca
     3361 ctgattgaag gagacgtagc aaaagtcggc gtaatcggga tggcaaaagg tggattagaa
     3421 ggttttttag caaaacaaca aactagattg aacgatatcg atttagggaa taagaaaaaa
     3481 atagagattg tcaatgcatt cttacctgtc aaacacttaa atgtagaccg tgtttcagaa
     3541 acgatttctt ctttagaaag agaaagagcg gaggtcttgg tttcttctat ggcatttgga
     3601 gtagataatg gggaaccaga acgagttgtt tatgaagcag catcggtaaa atcgatccct
     3661 acaacgatgg cttctgacat cacaaaatta tatggattga caagaagaac tcggacagca
     3721 gcaatcaatg catctatttt accaaaaatg ctagatacgg caacctctac agaagattcc
     3781 gtacgagaag caggggtcaa tgtttctttg atgattatgc gcggagacgg cggagtaatg
     3841 gaaatcaatg aaatgaaaaa acgacctgtt ttaaccatgc tttccggtcc agcagcatca
     3901 gtcatgggct ctttgatgta cttgagagcg tctaatggtg tttattttga agtaggcgga
     3961 acaacaacca atatcggggt cattaaaaat ggacgtccag caatcgatta ttcaattgta
     4021 ggtggccacc caacatatat ctcttcatta gatgttcggg ttctaggtgt ggcaggcggt
     4081 tcgatggtac gggctaatca gtcaggaatc atcgatgtcg gtccacgttc tgcacatatt
     4141 gccggtctag attatgcagt atttacggag acagaaaaaa tcaaaggtcc taaggttgaa
     4201 tttttctctc ctaaagaagg agaccctgct gattacgtca aggtagtgat ggaagacgga
     4261 gaagaagtga ctattacaaa tacatgtgca gccaatgtcc ttggattagt gcaagaagaa
     4321 catttttctt atggaaatgt cccatctgca cgaaaagcca tccaagcatt agctgattat
     4381 tgtcatacga cggttgaaga catcgcagaa cagatcatgg aaaaatccta tgctaagatt
     4441 gaaccggtga ttttagaatt ggcagacaag tatcacttgg agaaagatca gatttctctt
     4501 gtaggtgtag ggggcggcgc tgcctcattg attacttact ttagtaataa aatgggtgtg
     4561 aaatattcga ttcctgaaaa cgccgaagtg atttcttcta ttggtgtagc acttgcaatg
     4621 gtccgagatg ttgtagaacg tatcatccct tcgccaagta aagaagatat acgatcgttg
     4681 aaaaatgaag cgatgaataa agcgattgaa tcaggcgcta caccagaatc gatcgaagtc
     4741 cacgtagaaa tcgatccaca gacttcaaaa gtaacggcta tcgctactgg ttctacggaa
     4801 gtgaaagcaa ccgatctaac caaggagatc acaacagaag aagcactaga gcttgctgca
     4861 gaggatatgc gattaaataa aaatgaagtt tgcttattgg aaaatacacc tttcttttat
     4921 gtatgcggcg aacagaatcg ttcaaaaaat gctggaagtc ttcggatcat cgatcaaaaa
     4981 ggatttatca aggtccaacg tggacatgct tcatgtatga aaacaactgc agccaattat
     5041 atgacagctg tcgaacaact ttgggaagat atggcagttt atcaaactga attgattgcc
     5101 cgaccagaat tttatctctg cttaggagca agagtctctg attttacagc gacggatctg
     5161 gaacaactac agcttttgat ggatctggaa gtatctacta tggaaccgga ggaagaagtg
     5221 atcgtcgttg ccggaaatat caaacaaaca taagttagaa gagttactaa acgaattaca
     5281 acagatcccc gaagaaattt ggggattcta ccagtttcgg cgagatttat tttggaaaaa
     5341 gattcctttg tccaagcaga aaattcttat acaacaatca atcgattgtg gtatcgaaac
     5401 ggcatgtagc atcaaaaaaa agtacccttt tgccgatgta ggagaaattt gtgaacaaat
     5461 ggctgttcca atcgtttcct gtgaatcaga gcaaataaac gaacgaatca cttttgcaac
     5521 ttatgcagaa gatgaaggga ttcgattgat gacggagccg ttagaaaaat taaaatgttc
     5581 aggactagct tcgatctcta aagaaacagc gcaagctttg attattggac atgaactatt
     5641 tcaccacatc gaagcttctg ttaagggaat ttatacgcaa agcgaaaaaa ttgtgctgtg
     5701 gcggttgcct ttttatacac atcaatcaaa cattcgtgca ttgagcgaga tagcagcgat
     5761 gagtttttca aaagagatga atcaaagcga tttttcgcct tatgtattag aggcagtctt
     5821 actttggcca tataatgaaa ttcgtagcca agggatctta gaagaaataa aaaaaataga
     5881 aaaaagatgt acggagtatg atttagatta caaataaaga gactgtgaca aaagtcgtgt
     5941 cacagtcctt tttttccgaa taaacgatgt tctcctgtca actcctcggt cacaagtcaa
     6001 aatattcaac aaacatgaga agctgttttt tgaaattgtt ccttgtgact cggagatgaa
     6061 cggcaggctc acgacctctt ggaaaacggt gttcaaaagc ttgcatctgc ggtaataagt
     6121 ccagccaagt taaaaacata agaagctatt tttacttggc tgttcttatt acttgatgca
     6181 gggcagcttt ttcacaacct ctcaataaac ggtgtgcaat cagctgaccc tcggtattag
     6241 ctcaaaaaaa acggcaaaat atgaggaact attttgccgt ttctttctct aatactcagg
     6301 tcaaaacgct gattccaaaa cctcttattt gatgggtctg tcctgaatat cttcaaaata
     6361 atcggggtaa ttatgaatca cgtattcttg ctcctcgata atcatatgta aatgcatcgt
     6421 gcaaagaaat ccgacttcat caaagttatg atcaatcatt gattgaagca ttgtctgatg
     6481 ttcatctaat actcgacccc agtctaattc tgaaatcgtc aaacgtaacc atctgaaacg
     6541 gttcaagtgc gtactataac tttctagcca atcccaaatt tctcgttttc cagcaatttc
     6601 ataacaagtt cggtgaaaat cagtatccaa atcaaaaaaa gcttttttat tttgagcgat
     6661 tactgcctga tcggtttgtt ctagtatttt ttctagttta gcttgatctt gcttggtcat
     6721 tttcgcactg cattcttgca ggatcggttt ttccaacttt tcccgcgtat aacgggctaa
     6781 tgaagcagat cggatatcga tttttgaaac ataagtgccg gactgtggaa tggtttttac
     6841 aagctcttga ttgcgcagtt ggatcagtgc ttctcgaatc ggtgtacgcc cgatagctag
     6901 ttgttcttct aacactttat cggagatttt acttccaggt gctaactcgg aataaatgat
     6961 ttgtttacgt atagattcat aagcttgctg ttgcaaatta ttgataacca tttaaccttc
     7021 ctttctatgc agatataatc tattataaca taaatcgctt acaaatcgtg tgggcataaa
     7081 aacacagtct gcaaaaaaag ccctgatagg catgcctatt agagcctcga aaagaaaaat
     7141 attgaatcaa tctggtcgta cctcttgtat cggtttgaca cgaccgccga ttcttttagc
     7201 aaaagcttcg gattcttttt tgtctttgaa aatgagcaga ttgtctaagt ttttttcccg
     7261 agtgcgacag ccagttttat cgatgtaacc gtgcattact ttaacgacat acatggggat
     7321 cacctgcttt tttcttttca ttatacgaac tactcgacac aatacaagaa aaatgcttag
     7381 acggtcttta ttaagaattt acatcatcca aagaagcgta cgcttggaat aactgatcta
     7441 actccggatt gactttgctc agcttcacta atcgttgcga atcagaaagt aaaaaacagt
     7501 gtttactact gccagtagtt tttaattttt tgtcttcagt tcccacaatt tcgtagctaa
     7561 aatccaaccg tgtccccgta tacttttgga tagttggatg gatgctcact ttttctccgt
     7621 aatggatcat ttctttatag ctgcaattta cttccagaac ggggatgatg atcccagatt
     7681 cttcgatttt tttataggga aaccctagat gtaccaataa ggctacccga gcctcttcaa
     7741 accagcggat ataattgctg tgatgaataa tccccatttg atctgtttca taataaaaag
     7801 gttctcgcac ataacctgta aatttctcca tgactcatgc ctcgttttcc attgattttt
     7861 tacaaaaata attcatccat ttcggaagtg cttcgctgat ttgtgtcggc aaaacaatat
     7921 attgttcttt tgccagctgg tctccaatga aactatgaag ataaatagct gctgcaatcg
     7981 tttggtcatt ttgaggaaac tgggctaaaa atcctgtgat catcccagct aaagtgtcac
     8041 ccatgccgcc cgttgccata gcagcagaac ctaaaggatt ggtgtagaag tgctggtgac
     8101 cataaatttt tgtctcatga cttttcaaaa cgagcacagc accgatttcc tgttgcttct
     8161 ttttgttttc tttatcccgt tgttcttgta tcggtagaga actcaaccgt tgccactcca
     8221 tttgatgagg tgtaagtaca gtttgttcgg gatagggtag caaaagatga ttagcagcaa
     8281 gtaaacttaa agcagagcca tcaatcacca gccattgttc ttttgattgg ttttctagta
     8341 cttgtttgaa acgttccaaa ctcttagaag aagtacccaa tccgggacca atcaagagta
     8401 catctgcttg ctttattacg gaaagaaact ggtcagtatc cgtccaaact acaatcattg
     8461 cttctggcag atgactgtga agagcactcc gatttttttc atcagttact acagtcgtga
     8521 gacccgctcc gctgtgtacg gcagctaaag cactcataat gattgctccg ccatattgtt
     8581 cattgccgcc aatcaaaacg atacgtccaa atgttccttt atgtgaaatt tttggacgag
     8641 gtgtgatgat tcttttcact agttgttcgt ttatcagcat ctaaaaacct ccttttactc
     8701 attatagcgt atattttttt tagagaaaaa cagataagaa aaggaattgt cagatttttt
     8761 gaactatttt tttctgtaat ttgtggtatc atgaacacga agacttaaat aagtggagga
     8821 ttaactttat gacaattgat tggcaaaaag aagtagaagc acgtaaagaa gacttgcttg
     8881 aagacttgaa aaatctttta cgtgtcaaca gcgagcgcga tgactcaaaa gtaacaccag
     8941 atgcaccatt tggacctgga cctcgcgacg cattgaaaca tatgttagct tatggcgaac
     9001 gtgatggatt taaagtaaaa aatgtcgata actatgcagg gcatattgat cttggcgaag
     9061 gagacgaaac gctagggata tttggacaca tggatgtcgt tcctgcagga gatggctggg
     9121 acacagatcc ttatgaacca gtaatcaagg atggaaagat ctttgcacgc ggatcaagcg
     9181 atgacaaagg tccaagtatg gcagcttact atgcgatgaa gatcattaaa gaacttgact
     9241 tgaaactatc aaaaaaagtt cgcttcgtag tcggcagcga tgaagaaagc ggctggggcg
     9301 atatggctta ttatttcgaa catgaagaag aaccagattt tggtttctca ccagatgctg
     9361 aattcccaat catcaatggt gaaaaaggaa atgtttcttt agctttacgc ttcaaaggcg
     9421 acaatgcagg agattatgtc ttgaaatctt ttgtttccgg acttcgcgaa aatatggtgc
     9481 caggaacagc aactgcagca cttcaagtac caagtgcaga tgcagctatc gcgatggaag
     9541 aagcatttta ccaattcatc gaagcaaatc cagtaagcgg aacgattgaa gcggacaata
     9601 cgtatgtaaa aatcgaatta gttggtaaag gcgcacatgg tgcaagccca caatctggta
     9661 tcaatgcagg ttctttcttg gcattgttct tagataacta tgaattccta ggatctgcta
     9721 aacaatttat ccatgtagca gctgcttatg tccatgaaga tttttatgga gaaaaactag
     9781 gagtcgctta tgaagatgaa aaaatgggca aattgacaat gaatgctgga ctatttgctt
     9841 ttgaagaaaa tggtacagag gaagcgaact tcatcaatat gaatttccgt tttccaaaag
     9901 gtgtaacagt agacggattg caaagtgaca tcgaacaaac agtggggcaa gaaggcgcaa
     9961 cagttactcg aggtgctcgc gtaatggaac cacattacgt accaatggat gatccattgg
    10021 tggctacatt attgcaagtc tatgaagacc acactggaga aaaaggctac gaacaaatca
    10081 tcggtggcgg aacttacgga cgtttgttga aacgtggcgt tgcttatgga gcgatgttcc
    10141 caggatatac tgatacaatg catcaagcaa acgaatttat gagtcttgat gacttgttca
    10201 gagcaacagc aatctatgca gatgccatct atcgtttggc gaaataatta attaaaaaga
    10261 ggatcaagaa ttgcaaatta tttttgcagt cttgatcctt tttttacttt ctactgtggt
    10321 attcatgata aacatgatat agattcaatt catattcttt cttagcgttc gtgacgactg
    10381 tatccaaaat caaccctgtt actaatgaaa gaagtgctaa agtcatcaat ccagtactta
    10441 aaatcgcaga agggatgcgt gtaataaagc ttgtcagcat atattcgcga atcacaggaa
    10501 tccccgtgac caatccaaat aaaaagaaga agatggtcca aatcccaaag aaaagcaaag
    10561 gcttataatc tttaaacatt ttaataatca tcatgatcac tttgaatccg tcagagaatg
    10621 tattcaattt tgattcgctg ccttcaggac gatcacgata atcgattggg atttcaacga
    10681 acttgaattt tttatctaaa gcatggatcg tcaattccgt ttcgatttga aaaccagaac
    10741 tcataatagg aaaacttttg acaaagatac gattgaatcc tcgatagccg gtcatgacat
    10801 caagaatttt tgttttatac attctagtaa ttgtatttct aacaaggtta tttccaaaat
    10861 cgtggaaagg tctcttattc tcatcaaagt aggttccatt tgataggcga tcaccaatga
    10921 ccatatctgc ttcaccagaa cgtaatttct cgagcaaacc atgtacagct tctgcaggat
    10981 aagtatcatc gccatctacc attaaataat agtcggcatc gatatcaaag aacatttggc
    11041 ggatcacatt tccttttcct tgacgaggtt ctttttttac gattgctcca ccttgaacgg
    11101 ccaattcgta tgttcgatcg gtcgaattat tatcatagac ataaatatct gcttctggta
    11161 attctcgttt aaagtctgca atgactgttg aaattgttgc ttcctcattg taacaaggaa
    11221 tcagaacagc aattttttct tgttgttcca tttttcactc tccttaacta tagaaaacaa
    11281 aagctaaatt aaaaatacct cataatcaaa ggaaatgcta gtttttcccg ctaaatatca
    11341 tttatttaaa aagaaaatac tggaatatat gtttggaaag ctctatctaa aaaaccaccc
    11401 actacaaaag aaggacatga tacctctctt ttgaatagtg ggtgcgtatt cgttctctca
    11461 cttttttgac gtattaatct gttgcacctt tttttcttga aatttctaat acggttcctg
    11521 tatgagcatc tgctaaaaat tcataataaa cagtttcgcc atcttctatt cgagaaagac
    11581 ctccccgata agcactcgac cgaacggcga atttcctaat aggttccttt tcaaaagaga
    11641 tccaagaacc ttcgattgca ccttctttta gaaaagatga cttgatattt tcaagtactt
    11701 ggtctgctga caatactttt ttgcgatgat accaaatcgt tgaagctacc ccgctcaata
    11761 atcctagacc gattccaact gctaacccgc ctttaaaata gcttagttcg ctttcttcat
    11821 acatgtggac atacctcctt cttctcactt gttactcctt gtgcttattt taccacactc
    11881 tcatattttc tttgaaataa ataaccgcta ccagaaaaaa taagctataa taaatacgat
    11941 cggtctgcag aaaaaatcat attttatccg cagatcaata taagtctgag aggagtttag
    12001 tctgaaattt agacgaatag catatggaag aaaaaacatt tcaacgaatc aaagagttaa
    12061 cagaattaca aggaacaagc ggttttgaac aagatgttcg cgcttatatg gaaagtcata
    12121 tggaaccgct ggtagatgag ttacagttag atggtttggg cggcattttt ggtctgcgcc
    12181 atcatgaaga agcggatgca ccccgtgtaa tggtcgcagc acatatggat gaagtcggat
    12241 tcatgctgac acaaatccaa gaaaacgggc tttttaaagt cgtacctcta ggcggctgga
    12301 atccttacgt tgtttctgct cagcgtttta cacttaaaac ctctactggt aagaattatc
    12361 catgtatttc ttcttccgtt cccccacatt tattgcgagg aacaagcgga caaaaatcag
    12421 tagaagtaac cgatattttg ttcgatgctg ggtttgaatc aagagaagag gcaatgagtt
    12481 acggtgtcct tccaggagac acaatcgttc cttacgctga aacgatcaaa acagccaacg
    12541 gaaagaatat tatcagtaaa tcatgggaca accgctatgg ctgtacaatg gtattagaag
    12601 ctttagaagc gttgcaaaat gaaacattag gtcatacatt gattgcaggc gcgaatgtcc
    12661 aggaagaagt aggcttgcgt ggttctaaag cttctgtcaa taaattcaag ccagatctgt
    12721 tctttgcagt agactgctct gcagcagatg atacagtgac gaaaaatggc acattcggac
    12781 atttaggaga aggtacattg atgcgtatcc aagatccagg tttgatcatg cttcctcgtt
    12841 tgcgcgagta cttgctggat atcgctgaaa caaacaatat cccttatcaa tactttgttt
    12901 caaaaggcgg tacagatgca ggtgctgctc atacacaaaa cgaaggaatc ccaagtacag
    12961 tgattggcgt agtgggacgt tatatccata cgcatcaaac gatgttcagt attcgcgatt
    13021 ttgaagctgc acgagaaatg ttgattcaaa cactgaaagg tttggataaa tcaacagtga
    13081 atacgatcgt atacggaaaa taatgcgata gtgaaactat aaaatctggc aaaagattgc
    13141 tttttgccag atttttttta taaaaagagg atcaatgttt gttagttcga aagaaaggtg
    13201 tataatttaa ttgaaaaata cataaacaga aagtagagga caaagaatga ttatcccaaa
    13261 tagtttagaa gaattagcag gctacgtaga aactggaaag agtgtatttt tctttactgc
    13321 cggctggtgc ggagactgcc gttttatcaa accgcaaatg cctgaaattg aaaatgactt
    13381 ttctgattgg caatttattg aagtagaccg tgacaagtac atagatgtag ccgctgaatg
    13441 gaacattttt ggtatcccaa gttttgtcgt gatccaagac ggaaaagaat taggtcgtct
    13501 agtgaataaa gatcgtaaaa caaaacagga aatcgaatcg ttcctagaaa gtgtaacaaa
    13561 aggctgacat ctgttaggag gggtcaagat gaatcaagaa tataagaata ttttagtagg
    13621 tatcgacggt agtgaacaag cacttgctgc tttcaaaaaa gcggtagaag tggctcgccg
    13681 taataatggg aaagtatatg tagcaaacgt aatcgatcag caattttata actttatggg
    13741 ctatgcacct attgatcaaa atctagtcga tcagcaaact gaaacagcca aggaaatgat
    13801 cgaagaatgt aaagaatatg ggaaatctgt tgattacaca aatatagaag gaattattgc
    13861 atatggttct gttcgtgaat ccatggcagt caaattgcca aaaaaatatg agatcgatct
    13921 aatcatggtt gggcagtcag gtctaaatgc agtcgaacgc tttatgacag gaagtgtagc
    13981 cagctacgtg atacgtcatt caccttgtga tgtcttgatc gtttctgacg aagaagaaca
    14041 gccaagtaat taagcaagtg gaacttgtac gaccttttta atcaattaac taaaacagta
    14101 aagggactgt gacaaaagtc ttgtcacagt cccttgttac gaataaatgg tgttccagaa
    14161 gaggcttctt cggaaataaa gcatgaaaaa aatttcatgc agtgaatgca attttttgtt
    14221 aagataaatc tcagcaaagg ataatggagg aaagaacatg atttttgctt ataacaaagc
    14281 acacgtcgga gacaccttga tggttattat agccgacgat aaaggaacag aaaacactgt
    14341 tgaacgcaaa tggaatgttg ctcggatcaa agatgaacaa ggtcaagttg ttgcttggaa
    14401 ctttttccat atctccgatc atctaacaat cgaagggaac ggccaagtaa gcttaacaga
    14461 tgaggaatta gcgaaattga atcgattgat caaagaagca ggattcgaag aacagcttga
    14521 agctgatcat gaaccgaaga tcgttgtcgg gtacgtaaaa acatgcgtcc cacatcctga
    14581 ttctgaccat ttatcgatta ctgagacaga agtagacaat ggtacagtat tgcaaatcgt
    14641 ttgcggtgca cccaatatcg aagctggaca aaaagttgtc gttgcaaaac caggagctat
    14701 gatgccagat ggaatgatga tctggccagg aactttgcga ggggtagata gctatggaat
    14761 gatctgttcc gctcgtgaat tgcatttgcc aaatgctcca gcaaaaaaag gaatcttagt
    14821 attgcctgag gatgccgtag tcggagaagc atttccaaaa taaagaggtt gtgacaagtc
    14881 tttgtcacaa cctctttttt attaattttc actattttga ttttgtttta atgccgattg
    14941 atcgatcgtc aattcaacag tagttgtttt ttctttcgag ccttcatagt aagtgatttt
    15001 gacagaatct cccacttttt tcttatacaa agcagattga agttctacac cagaagaaac
    15061 ttctttgtca tcgatcttag taatgacgtc gtattgtttc aatccagctt tttcagccgg
    15121 tgtagctgtt tggacatttg tcacgatgac accgttagtt acagatgaag gaatcttcaa
    15181 gatttgttct tgttgctgag tagaaacggc agataagtcg accattgtga tacctagagc
    15241 tggacgtgtc acttttccgt ctttttccag ttgattgatg atattcacta catcgttact
    15301 tggaatagca aatcccattc cttctacgct tacattagaa gtagattcag aagtacttgc
    15361 gatcttactt gagttgatac cgataacttg tccttcgata ttaactaaag gaccaccaga
    15421 atttccaggg ttgattgctg catctgtttg gatcgcattg atattgactg tttcgttcga
    15481 ttcattcgta cttgtcactt gtcggttcaa ggaagaaatg attcctgaag tgacagaatt
    15541 tgcatattca gatcctagtg gggaaccaat cgcaatcgca ggctcaccaa ccttcagagc
    15601 acttgagtca ccaaaagagg caactgtttc gactttgtct gaattgatct tcaacaccgc
    15661 taaatctgaa taagcatctg ttccaactaa ttcagctttt accttcgtac cgtctttcat
    15721 caagacttca agaccttgct ggccatctac tacgtgatta ttcgtgacta cataagcagt
    15781 attcccgtct tttttgtaga tcacgccgct tccttcacta taagcttcca aattactatc
    15841 atctgagctg ctactttctt cttgctgccc gaatagtccg ccaaaaccgc ttgattggtt
    15901 ttgactttgt aagttgataa cagaaacgac agcgtcttga actttatcta ccgctttggt
    15961 gatatcagaa tctacattga ctttgatatt ttccacaact gtttcaccgg cagaattctg
    16021 attgccgctt gtagcagtag aactattgtt gccagtgccc atcgctgcat aaaaaatgcc
    16081 agctgtcagt agtcctccaa cgatccctcc aacaagcccc agacctaact tacgccagag
    16141 gctattattt ttactttttt tcatctttgg tgtcacattt tttctatcca tgtgttcctc
    16201 ctccaattac attcatttat ctactgttag tatagtctta tttgttgact ttagtctact
    16261 ttgagactgt gaacttttta tgaaaaactt taggaaaaat cattgttttc agatgaaaat
    16321 atttgatttt ctttttccac agatactgtg taaaactacg cggataggat tggaaaaata
    16381 agggataaaa catatccaca gcctgtgtat aaagtggata aattcttata aaacgtatgt
    16441 ttgcctaatg aatgtgatga aataaggtaa aataacactg ttttctgtgg ataaacctgt
    16501 ggatgttgtg cataactgtt ggggaaatct ttttaacaaa aatagccaaa ataaacatat
    16561 agacgaaaaa attgattata ataaacaaag tgagcatgaa gaccaaagga gagaaaacat
    16621 gaatatcaaa attatctcag taggaaaatt aaaagaaaaa tacctgatcc aaggtatcaa
    16681 tgagtatgtc aaacggctga atgcctatgc aaaaatcgaa ttgatcgaag ttcctgacga
    16741 aaaagccccg gagaatttaa gcgaagcgca aatgagacaa gtaaaagaaa aagaagggga
    16801 acggatctta gcgaaaatca aagaacaaga atacgtctat gccttagcaa ttgaagggaa
    16861 aaatcctaca agtgaatctt ttgctaaaca aatcgaccaa ttaggtatcc aaggaaaaag
    16921 ccaccttgtt tttgttatcg gcggctcttt aggactcagc gaagccgtca tgaaaagaag
    16981 caatgctcag atctcttttg gaaaaatgac gtatccacat caattgatgc gtctgatcct
    17041 tgtcgagcaa atctaccgtg ccttccggat caatgcgggg gcgccttatc ataagtgatg
    17101 cggtttttat cattgatcct aaaaatctta gcaagacaaa aagtacgtaa gaaagtaaag
    17161 actaaatact tcggaggaac aaatgaataa caatgatagg ttattgcgat tgcgttatgc
    17221 gattgatttg aaagatacgg atttgattaa agcttttgag ctaggcggtg taccactgac
    17281 taaagaagaa gctcaggcgg tactgaccaa agtacaggat aagaacaaag ataatgcaga
    17341 aaataatgtg tatgaaaaaa ccatcaacaa ccaagttttt gacgcattcc taaatggcct
    17401 gattctcttg gcgcgtggtc cgcaaaaaga taagccgata gtagacacgc cgcaaaaaag
    17461 caaggaaatc aaatatatca acaatgtgtt gctaaaaaaa ttaaaaatcg ccttgtctat
    17521 gaccactgat gatattttag acgtttttgc tgaggcagaa atctatccat caaaaggcga
    17581 aatcggggca tttttgagaa aagaaggcca acgaaatttt aagccttgcg gagataaata
    17641 tatgcgtaat tttctcaagg ggttaggaat ttataatcgg agaaaagtat aaagaaattt
    17701 catgagaaat caaaacaatg gttgttttga tgagagctag tttttgtatg agattaaaat
    17761 attatcttaa tcgaggagct ggattggttt tatgaatgat gaatataaga atgatgaaga
    17821 taagatgctt tttgaagaga tagagaatcg atgtaggctc aattttgagc ttcggggaaa
    17881 gatgtcttta atacaacaaa aaaagtattt agcgaataaa tcagagttta ctctgggaca
    17941 tgttgaaaaa ttaatctcgg attggattag tagtagaagc gaatttacaa aaataaaaca
    18001 gccgattaaa tttgacatga aaaaactttt gttaaataag agtgagatag gtaatagaga
    18061 tcaatatatt agagctaaag ggcaagaaat aattgattca ttaggtgaaa tgaggtcata
    18121 caattatttg tatgtaacac atagagctga tggaatggtc attactgtgg ggaaatcttc
    18181 ttctaatgat atttttttag atggagatct tttttatcaa ctcaatacaa atcacttgtc
    18241 tggcactgaa aatataattt taaggacgga atatggcaat gaaatatttg caaaatatga
    18301 tgagatttta aaaaattatt tggactgggc ttggataatt ccggttgagt caggagatgc
    18361 taagaaactg gagagattat tgggcgatga actaattaat aaaaaagtac caattttaaa
    18421 ttattattcc catagacaat aaaaggctat ttttcatgat aatactagtt ataactgtaa
    18481 aaatggaaga aaatctggga aagaagtttt taaataagtg tattgttcga ttttaataaa
    18541 taataagcaa taccatccgt ctgtgttgcg caacatcgta ctacaatgaa tattttttca
    18601 caaaaagaaa atcatccatc gaatgaatgg aaggttttag tatacacgag gttaatgccc
    18661 cctcttttat aatcagagga gtgaggtttt ttctatgaat gtttctattt tcttattgct
    18721 agtctagttt ctcttacgat gaaattggga ttctattcca gtttttattg atttgctgat
    18781 aaaataactt gaatggtgga tataaacatg ataaataaga gaagaataac aaaacaaggt
    18841 tgtgaaatga ttctcaaatg aatataatag aaagaaaaag gaggaagaat caatgaaaat
    18901 agcaattgca ggcgctggcg caatgggaag tcgctttggg ttaatgcttc accaaagtgg
    18961 gaacgaagta ttattaatcg atggctgggc agaacatgtc caacaaatca aagagcatgg
    19021 attacaagca aattttaatg gtaaagaggt agaagcaaaa ctaccaatcg ttcttcaatc
    19081 cgaagtagaa aaagaagatc aagttgatct gattattcta tttaccaaag cgatgcagct
    19141 ggaaaaaatg ctgcaggata tccaatcatt aatcaaaaaa gatacagagg tcttatgtct
    19201 attaaatggt atcgggcatg aagatattat tgagaaattt gtaccaatgg aaaatatcta
    19261 tattggaaat accatgtgga cggcaggtct tgaaggtcct ggtcaggtca aattatttgg
    19321 aagtggttca gtagaattac aaaatttagg tgatggaaaa gaagcagctg caaaaaaatt
    19381 agcagacaaa ttgtctgaat caggattaaa tgctcatttt tctgacaata ttcactattc
    19441 tatttatcgc aaagcatgtg tgaacggaac aatgaacgga ctatgcacaa tcttagacgt
    19501 taatatggct gagctaggaa aaacatcgac tgctcataaa atggtggcga cgattgtcaa
    19561 tgagtttgcc aaagtagcag cagtagagaa gattgaacta gatgtcccag aagtcattgc
    19621 acattgtgaa tcttgttttg acccggaaac aattgggtta cattatcctt caatgtatca
    19681 agacttgatt aagaaccatc gattaacaga aatcgattat atcaatggcg caatttctag
    19741 aaaagggaag aaatatggtg ttgcgacgcc ttattgtgat ttcttgactg agcttgtcca
    19801 tgcaaaagaa gatagtttga acgtaaaata aaggagagat tgaatggaaa cttcacaaaa
    19861 aatatcaccg aaaatttttc tgaataaagt cctcgctggt acagcaacag gaatcattat
    19921 tggattgatt cctaatgcag tattaggtgc cattttaaaa tattttagtg cgtatccctt
    19981 tgcggtaacg atcgcacagt tagcagttat ttttcaacta gcaacacctt tgatcattgg
    20041 tggtttgatt gccttacaat ttggatttga tccgatgaga atgatggttg tagccggagc
    20101 ttcttttgtt ggttctggtg tagtcaaatt cacacctgat ttgggtgaaa cgggtgttta
    20161 cgttggtgct ggtacaggcg atattattaa tacgatgctg acagcatcga ttgctgtcgg
    20221 gctgatttta ctgattggaa atagttttgg gtcagtgtcg attgtgttaa caccgattgt
    20281 tgtaggtgta ggtgcaggtt tacttggttt ttatttattc ccttttgtaa cagcaattac
    20341 ttctgcaatt ggatcattga ttaataactt cactacctta caaccgctgt taatgtctat
    20401 cttgattggt tgttcctttg cctttttgat tatctctcct atttcaacag tagcaatcgg
    20461 aatggcaatc caactagatg gtgtttctgc tggtgcggct gcgatgggcg tagctgcaac
    20521 cacttttgtt ttagtagtga actcttggaa ggttaataag tcaggtgtga caattgcgat
    20581 tgctttaggc gctatgaaga tgatgatgcc taatctattt agaaaacctg tgattcttat
    20641 tccttgttta gttagtgcag tgattacagc tattcctgtc gctctatttt ctatttcagg
    20701 tacgcctgct tctgctggtt tcggcgtggt cggacttgtt ggtccgttgg cttctttaga
    20761 tgcagggcta aatggctttc ttgttgttct ttgttggtta gtcatccctg tttttgcggc
    20821 actaattact caatttttat gcgagaaagt atttaaactg tatgatcgag aagaagtctt
    20881 caaattttta ggctagtaga ttttactaag aaattttcaa tcaatggatg cgtaagtgag
    20941 atgtcaacaa catctgactt acgttttttt gcctttttga gtaggagatt gatgctgaca
    21001 actgaactct tcagtgccat tcgatttttt aaaatgtaga agaaactcac ctttatgtta
    21061 ttgattgttc aaaaggcaac tagaataaaa atatgtgtta aaatgagaaa aaatttgatt
    21121 gttggatgta tcgaataatc actaaaaagt aggagaagaa aatgattttt ttgcaaaaat
    21181 atggttttta ttttttatta ttaggtgtga ttagtgattt atcgacaccg tatatccttg
    21241 gcctttttta tcccaaactg aatcaaatga ctaccgtaat tagtgtgttt ggtgacgtag
    21301 atagtccagt cagacgtgct ttcttggtct ggtcagttgt atctggactt ttttttgttc
    21361 tttcgttgcc tgcgttgtat cacctattta ttggaacatc aaaaacactt gcaatacttg
    21421 ctgttgcaac agttggttta tatgggatag gtgattgtat ttttactggg ttattcagta
    21481 tcaatacgaa tgaaagctct tggaatctat cgacttggat ccataatata ggttctgggc
    21541 taggctatgc aggctttcta cttttcccat tgttacttgt tctgctttat cgtcaaagtg
    21601 gtcaaggtac gcttagccat ttttatcttg ttttaacagt tattagttta ctaattgcag
    21661 gtctttacag attagcacgg attccgtcga ttagtcagtt tgccttcttt aagcaattgg
    21721 gcttctttca acgcttaagt tttttcttta actatttgcc gatgatttgt ttaagttttt
    21781 ttcaactacg aaagtagaaa aagcagtgtg aagctgatta agtaaaagaa tttctctatg
    21841 ggtaaccatt cgtgtggatg tgttcgacta agaatagaga tgaggagagc gaaaagcaag
    21901 ttagtcttaa cttgttatgc gttgtgctaa atttcaggta gtcatttttt gaatgaataa
    21961 gaagaaaact tgaaagaggg tgacttcatg ttagatgtgg agcagatgga aatagacctt
    22021 tataatatta ttttaaatcg aaatacaaga gaatgggaaa gaacacaatt tgaagaggca
    22081 aaagaagacc tagcaaaaat gtcgataaag caggtgatag aaaaactaga ggtcaacttg
    22141 cgtccgctag cactacgaga taatttgaca ccagatgcga tggattttta tttaagaatc
    22201 acaggggagt cagcgagtgc aaaggcttat gattatgcca aacataagat aacttcaccc
    22261 ttataccaag aacgggcgat ttttgccggt ggctgtttct ggtgtatggt tgagcctttt
    22321 gaaacaaaac caggtgtaat ctcagtgtta tctggttata caggtggaga gaccaaaaaa
    22381 ccaaattacg atcaagtcag tggtggatat actgggcatg ttgaagctgt tgagatcata
    22441 tttgatacac gtcaaataac ctatgaagag ctgttggaga tttattggca aatttcagac
    22501 cctacagatg cctttggtca atttcaagat cgggggaaac aatatcgttc aattctattt
    22561 gttgttaatg aaaagcaaag gcaattggct gaaaaatcaa gagaacaact gattcaatct
    22621 aaccgattta gccgtcctat cgttacagag attcgccaag caaagacttt ttggccagct
    22681 gaaaatcatc atcaacaatt ttatcagaag caacctaaaa gatataaaaa gatcaagcat
    22741 tcgcatcagc ttcatttatt ttttcagcga catagataga ggaaggttgt ggcgcaaagc
    22801 gttcagctcc gacaaaataa tgagcaactg acaaaaatag cttttcatat ttttggacag
    22861 ttatgaatta tttcgaggag ttgctttgag aacaccgagg aaaaggttgt ggcgcaaagc
    22921 attcagcttc aagtcgcaag gaaacaggag gtatgacaag tcttttgtca caactcctgt
    22981 ttttttacta gcctttattt ccatgtgaaa gagaattcga attgtagaat cttgattttt
    23041 ctatggaatt ttgtagagaa tcatcttgat aattaataga cacctaatcg aattccacat
    23101 taatgatgaa ctaaattccg tataaaagcg caaacaataa ctata
//
LOCUS       DAFEOM010000035        22856 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.036, whole genome shotgun sequence.
ACCESSION   DAFEOM010000035
VERSION     DAFEOM010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.036"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(104..1336)
                     /locus_tag="LP524_001601"
     CDS             complement(104..1336)
                     /locus_tag="LP524_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBL2234660.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="LP524_001602"
     CDS             1591..2160
                     /locus_tag="LP524_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL2234661.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="LP524_001603"
     CDS             complement(2338..2778)
                     /locus_tag="LP524_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBL2234662.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="LP524_001604"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="LP524_001604"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBL2234663.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="LP524_001605"
     CDS             3732..4655
                     /locus_tag="LP524_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBL2234664.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4731..5870
                     /locus_tag="LP524_001606"
     CDS             4731..5870
                     /locus_tag="LP524_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HBL2234665.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5863..6663
                     /locus_tag="LP524_001607"
     CDS             5863..6663
                     /locus_tag="LP524_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL2234666.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6663..7490
                     /locus_tag="LP524_001608"
     CDS             6663..7490
                     /locus_tag="LP524_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL2234667.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7468..8202
                     /locus_tag="LP524_001609"
     CDS             7468..8202
                     /locus_tag="LP524_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL2234668.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8302..9168
                     /gene="rfbA"
                     /locus_tag="LP524_001610"
     CDS             8302..9168
                     /gene="rfbA"
                     /locus_tag="LP524_001610"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBL2234669.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9182..9754
                     /gene="rfbC"
                     /locus_tag="LP524_001611"
     CDS             9182..9754
                     /gene="rfbC"
                     /locus_tag="LP524_001611"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBL2234670.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9776..10804
                     /gene="rfbB"
                     /locus_tag="LP524_001612"
     CDS             9776..10804
                     /gene="rfbB"
                     /locus_tag="LP524_001612"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBL2234671.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10902..11753
                     /gene="rfbD"
                     /locus_tag="LP524_001613"
     CDS             10902..11753
                     /gene="rfbD"
                     /locus_tag="LP524_001613"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBL2234672.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11787..13820
                     /locus_tag="LP524_001614"
     CDS             11787..13820
                     /locus_tag="LP524_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234673.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13864..15143
                     /locus_tag="LP524_001615"
                     /pseudo
     CDS             13864..15143
                     /locus_tag="LP524_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
     gene            15353..16159
                     /locus_tag="LP524_001616"
     CDS             15353..16159
                     /locus_tag="LP524_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2234674.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16171..17391
                     /locus_tag="LP524_001617"
     CDS             16171..17391
                     /locus_tag="LP524_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2234675.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17381..18967
                     /locus_tag="LP524_001618"
     CDS             17381..18967
                     /locus_tag="LP524_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBL2234676.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19006..21144
                     /locus_tag="LP524_001619"
     CDS             19006..21144
                     /locus_tag="LP524_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL2234677.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21512..22495)
                     /locus_tag="LP524_001620"
     CDS             complement(21512..22495)
                     /locus_tag="LP524_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBL2234678.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
BASE COUNT         7176 a         3899 c         4711 g         7070 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     4741 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     4801 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     4861 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     4921 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     4981 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     5041 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     5101 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     5161 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     5221 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     5281 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     5341 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     5401 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     5461 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     5521 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     5581 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     5641 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     5701 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     5761 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     5821 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     5881 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     5941 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     6001 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     6061 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     6121 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     6181 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     6241 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     6301 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     6361 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     6421 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     6481 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     6541 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     6601 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     6661 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     6721 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     6781 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     6841 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     6901 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     6961 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     7021 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     7081 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     7141 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     7201 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     7261 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     7321 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     7381 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     7441 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     7501 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     7561 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     7621 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     7681 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     7741 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     7801 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     7861 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     7921 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     7981 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     8041 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     8101 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     8161 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     8221 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     8281 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     8341 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     8401 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     8461 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     8521 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     8581 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     8641 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     8701 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     8761 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
     8821 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
     8881 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
     8941 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
     9001 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
     9061 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
     9121 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
     9181 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
     9241 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
     9301 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
     9361 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
     9421 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
     9481 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
     9541 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
     9601 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
     9661 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
     9721 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
     9781 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
     9841 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
     9901 agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
     9961 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    10021 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    10081 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    10141 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    10201 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    10261 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    10321 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    10381 agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    10441 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    10501 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    10561 attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc
    10621 aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa
    10681 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    10741 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    10801 ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    10861 tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag
    10921 gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg
    10981 tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca
    11041 cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag
    11101 aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac
    11161 ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg
    11221 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    11281 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    11341 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    11401 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    11461 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    11521 atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag
    11581 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    11641 cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt
    11701 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    11761 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11821 aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11881 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11941 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    12001 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12061 tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa
    12121 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12181 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12241 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag
    12301 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12361 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt
    12421 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12481 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12541 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12601 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12661 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12721 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12781 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12841 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12901 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12961 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    13021 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13081 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13141 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13201 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13261 tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13321 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13381 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13441 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13501 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13561 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13621 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13681 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13741 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13801 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg
    13861 agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13921 ttgcttttgt tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca
    13981 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    14041 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14101 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14161 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14221 gatcgcggta tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt tatcgtgtat
    14281 ttacagagat ccaagtacca gaaggacaaa gcatctgggc tccaggcaat tcgctttctg
    14341 atatttggat caatacgaat acaatcggaa gttctttgat gactcttgct ttattgatca
    14401 gtggatttgc cagttctttt gaacgatggt atgtacgaat catcggtgca gtagcagttg
    14461 ttgcagcttt tctcgggatt tgggtctgcc aatctagagg tgcattagtc gcgttgattg
    14521 tttttgcgat attggatctt ttacctaaat cgttgatgcg tgtcattcgt gcgccattta
    14581 ttgcttacac ggttacgatt cttttagctt tgccgatttc ttatctggcg gctgtctctg
    14641 aaaaagtcaa tttgtttacc ggtcgagaag atatttggca taaattctat cagacgctag
    14701 gagaaaaatc tgaacaaatt ttactaggaa tgaaaacatt tatcttccaa cgaggaaacc
    14761 aatttttagg aaaccacaat agttataatt ctattttgaa tatctatgga ttgataggat
    14821 ttgggattgc tgctttgctg cttatccttt ttattggtcg cttaacgttg aaagcagatt
    14881 tatccaacgg gcagatgact tttatctggg ccttttttgc ggtgatgatg cagtcattta
    14941 tggaggatac attgacttca taaccatggg ttccgattat ttatatcctg ttggctatgg
    15001 ccgttcatcg atatgatgaa ccagaagttg cggaggaatt ctatgttatg gaagaggcat
    15061 atgacgatat gacttatgat gatacatctt atgaggaaca accagatagc gctcattctt
    15121 ccaggatcca acgctatcat tgataggcac aaaatacgag agaggctgtg acaaccgtcg
    15181 cagccttttt tggtttattg caattgtcta gcatcttctg taaaatagaa acgttagaga
    15241 aaatcttgta tcatagaaaa ttaagaaaga aataagaacg cagctcggta ccaaaaatat
    15301 ggtaaaatgg cacttgactg taattcactg aaaatcgctt ggaggaacac aaatgtttaa
    15361 agatttgtat ttatttatga aggacattta tcagaacaga aaattattgc tacagttttc
    15421 gatcaatgac ttcaaatcaa gatatgcagg atcgttttta ggaatcttat gggcgtttat
    15481 caatccgcta tttactgtat tgatttattg gctggtattt ggtgttggac tgaaagcaca
    15541 aatgatcgat gcaaaatacc catttatcgt atatttagtt accgggatga ttccctggtt
    15601 cttcttctca gatgcatttg catcaactac ggttgttttt cgggaatata cttatttagt
    15661 aaagaaagtc gtattcaata ttcgtatctt gcccacgacg aaaatcctat ccaatctgta
    15721 cacccattta ttcttcattc tgattggcat agctgtcgcg cttataaatg gtatatatcc
    15781 ttcagcaatg tctttacagc tgatttatta tttattttgc atgtgtgctt tcttgacagg
    15841 attgacttgg ctgacggcat cgatccaacc atttttacct gatatcatgc aattaattac
    15901 gatcgtgctg cagttgatca tgtggacatt accgatatta tggagcccta atcagtttga
    15961 ttcaggtctt gtaagcatat tgaaattaaa tccgctgtac tatgttgttc aaggttatcg
    16021 tgagtcattt ttaagtgaag cttggttctg ggagcatgga acatatactt tgtatttctg
    16081 ggttttcacg ttagtaatgt tcgtaatcgg ctctgcagtg ttccgtagat tgaaaccaca
    16141 tttttcagat gtgctgtaag gagaatagct atgacagaat atgcaattag actaaaagat
    16201 atcacaaaat cttataatat gtacgcaaaa ccttcggatc gatttcgtga agccctcaat
    16261 ccttttaaga aatcctatca tgatgtattt tatgcgttga aaaacgtgaa tgtagaaata
    16321 aaaaaaggtg agatgatcgg tttcgtggga gaaaacggat cgggaaaatc tacaatgttg
    16381 aaaatcatta cgggagtact gacaccaaca tccggcacga tggaaatcga aggaaaaatc
    16441 tcagctttgc tagaactagg atcaggattc aatcctgagt attctggcta tgaaaatatc
    16501 tatttaaatg gaatggttct aggattttca agagaagaag tcgatgaaat ggtcgatgat
    16561 atcattgaat ttgctgacat cggagaccat atttatcaac ctgtaaaaac ttattcaagc
    16621 gggatgttcg ttcgtctagc ttttgctgta gcaatcaatg tgaatcccga tattttgatt
    16681 gtggatgaag cattggcagt aggcgatctt gaatttcaac taaaatgtat ggaaaaattt
    16741 acagaaatca agaactcggg aaagacgatt ttattcgttt ctcatgatat cagttcgatt
    16801 cgtcgttttt gtgatcgttc ctattggttg aaaaacggag aagtcgtcga atatggcgat
    16861 acgatggaag taacgacgaa ttatgaaaac tttttaaaga aaaaatcagt taaaacagtg
    16921 gatcggaata aaaaccaagt agaacggttc gccgctccag atatcgtcga cgtgacgaaa
    16981 gcggaattgt tggataaaga caaaaatccg gtggatatgc tggaacaagg agaaaaacta
    17041 tacgttaaag taacttatga agtaaaagat gatacaatca aaaatccggt tttaggaatt
    17101 gcgctacgaa cagtcgacaa tttttatgta tgcggactta atactctact agatggtatc
    17161 aagattcctt ggaaaaaagg agagaatatc ttttatctgg agtatgaaaa gttaggtttg
    17221 ttagctggtg aatattattt tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg
    17281 gtatataaga caaagtacat gaacttgttt gtcagcggtt cttatatcgg agaaggcatc
    17341 gtggtcttag accataaatg ggaagaaggg actcatagca atgaaatatg attttgaaat
    17401 ggacctagat gaacaaagca gtgtgggaaa aattgccgca cagatcaaac caggaagtaa
    17461 agttttagag tttggtcccg gaaacggccg attaacgaaa catttaatcg gagcaaaaca
    17521 atgtgaagtg agcattgttg agttagataa ggaattgttc gattttgtca gcgaatttgc
    17581 tcaagatgga ttttatggtg atatcgaaag ttttgagtgg gcaaactatt atgctggaca
    17641 gacatttgat tacgtcttgt ttgcggatgt tttagaacat ttggtagatc ctggaaaaac
    17701 attgaagaaa gtcagagaat ttttgaatga agaaggagag atcctgatta cattccctaa
    17761 cttggcgcat aattctgtca tgatcgattt gttcaataac caattgccgt gggcttctta
    17821 tggtctccta gatgaaaccc ataattcttt ttatactcat gatggcttcc aaaaagtttt
    17881 tgaaaaagcg ggattattca ttaatattga agattatttg tacttagctg taggtgatac
    17941 agaattaaaa tcaacttatg aagaattgcc ggaagctgta cgctatgatt tcaaaatgcg
    18001 accatttggt gaagtctacc aatacttttt ctcattgatg aaacatccag tagcacaaag
    18061 ttccattgca gagccacaaa attctaatta tgtaaaagtg ttagaagtaa cccagcaaac
    18121 gaaacaagat gaaacaatcc aacaaatacc attcaataat ttcacagggg aaaatgagac
    18181 actaagtttc cccgtatcag aaacaacaga aagaatggtg tttcgcttcg cgcaacaacc
    18241 tagctttatc gaattcagcg cagaagctgc cggagaaaaa ctaacattta tcgattcgaa
    18301 tgctgtagtc aaaacagtaa atgattgtta tttgtttgat gggaaagaat taccagagtt
    18361 cgttctaacg gatatttctg ggaaagaagt gactatccat tgtcactatc gctttatcgg
    18421 ggaattgaca ccaacaatga aagaattgtt ggaaacgatt cgtcccatgg ctgaagtcac
    18481 aaagcagctt tctgagaaaa atgacgaatt agaaagagaa aatcatcatc ttcttgagga
    18541 aaatacaaga ctagaccata cgttaaaaac aaccacaaat cgctattgca cattgttgga
    18601 aagtgatgaa tggacaataa aacatagact aggacgacgt aagaaagaaa cttcgaaaaa
    18661 aatccaagaa aaagaacttt ctatatgtat tgacgagaaa atctgggatg cagaaacaaa
    18721 aatattaaaa atcatcggat ggggaatcgc taattcggat cgccagccct tgtcctataa
    18781 gttatcagct gaccaatcgc catttttcga agcaattcct gtttcacgag aagaagtcaa
    18841 tcaagcagaa cagctcgcaa aaggaacaga agcgggattt gagttacgaa tcctttgcga
    18901 gcaagaacgt tcattcttag tggaagcagt agctcaaaat ggccaatcat ggatcattga
    18961 aatgtaaata atgattaact tataaagata aggagtaaac gaatcgtgca aaaaaaagaa
    19021 attgccgtat ttatcgatca aatcactcgt gaacgggcca ctggcgattt gcttattgtg
    19081 ggctgggcaa ttgatgaagt gaccaaagaa atccctacga taaaggtaga aaaagaaaat
    19141 gtgattgctg aagcaacaca tgtcgtaaga ttggatatca atcatttata taatcttgat
    19201 gttaagaccc aaagcggttt taaggtacgt ttatcaggga aaatgagagg aaaagccatc
    19261 ttagacttcc aaacagcgag acatcaaaat ggcatagctg tgaaattaaa cggtaaatac
    19321 ccttatgatg acggcattga atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg
    19381 ataaattatg caaggactca tggcgtaaaa aaagcgatcc gtcgggtcaa attggaattg
    19441 aagccaggat cgatcgacta tgcagagtgg atcagtcgtc atgagaaacc agatttgaaa
    19501 gagcaaagga aagaagcaca aggatttgcg taccgtccat tgatttccat acttatgcct
    19561 gtctataacg ttgaaatcaa atggctcgaa aaatgcattg attcggtact ggatcaaacg
    19621 tatgatcatt gggaactatg tatcagtgat gatgcttcta ctgatccagc tatccgaaag
    19681 tgtctggaat cctatcaagc aaaagatgat agaatcaaag tcgttttccg aaaagaaaat
    19741 ggacacatca gtctggcaac taattctgca ttggaaatag cagaaggaga atttatagca
    19801 ctcttagata atgatgatga attaccgcca tttgcattat atgaagtggc aaaagtgcta
    19861 aacgtacatc cagaactgga tgtgatctac agtgacgaag ataaaatcga tgcagatggc
    19921 aatcgctttg atccgcattt caaagcagac tggtcgcctg atacattgat gggaaataat
    19981 tatatctctc acttaggcgt atatcgaaca agcattgtta aagaacttgg cggtttccgt
    20041 aaaggctatg aaggttctca agattatgat ttggtccttc gtgtgacaga acaaatccct
    20101 gcagatcata tttaccacat tgaccgagtc ttgtatcatt ggcggacgat cccaggatca
    20161 actgcaagta atggcgaagc caaaagttat atctatgatt ccggagtgaa agcattgaca
    20221 gatgccctgt ctagaagaaa catcaaagga agcgtacgtc ctggcagaat ttctggattt
    20281 tatgagattg cttatgacgt cttgcaagag gatttggtca gtgtgatcat tccaacgaag
    20341 aatggctatg aagatctaaa aacttgtgtg gattcgatca tcgaaaaaac gtcttattca
    20401 aattatgaaa tcattattgc ggataacgga agtacagatc caaaaatgca agaattattt
    20461 gcagaataca agcatcagct gaaagaccgc tttatcgtag aattgattga cattccattc
    20521 aattattctc gaattaacaa tttagcagca gaaaaagcaa acggcaaata ctttttattc
    20581 ttgaataacg atacagaagt gattgagcca gattggatga cggtgatggt ttcttatgca
    20641 cagtttgatc gaatcggctg tgtaggagca aaactgtttt atccagatga tacaacgcag
    20701 catgcaggag tattattagg aatcggtggt gttgcagggc atgcattgaa taactatgat
    20761 cgaacacatt gtggttattt cggccgattg gtcattgacg tcaattactt agcagtcact
    20821 gcagcttgta tgatggtcaa ggcagctgac ttcaatgctg ttaacgggtt tgatgaaaca
    20881 ttagaagtag cattcaacga tgttgacttg tgcttgaaag tttatgaact gggtcgttac
    20941 aatgtctatg cacatcaagc agaattgtac catttcgaat ctaaatcaag aggttatgaa
    21001 gatactccag aaaaacaaag acgttttgct ggtgaaatca aaaaaatgca agataaatgg
    21061 ccagcatata ttgcgcatga tccattttac aatgataatt taacaaaaga aggaatcggc
    21121 gacttttcgt tgagaccaga ttaaactata ataaaaagct gttatgttgt gattcgctcg
    21181 cacataacgg ctttttatag ttttttgtca aaaaaaggca gaaggtgccg actcaaaaaa
    21241 ataagtcgag gttgggacag aagtgtttaa ctccgagaaa taagaagaaa ttcacgaaaa
    21301 ttgcttttca aatttttgtg aatttcggct tatttaagaa gaagttgctt ctgctcccac
    21361 cgtttattcg tttttaaaga atgagacaaa agtgtttctt acttttgtcc cacgctctta
    21421 ttcatgctca gagcgccaat agatacaaaa tcatagccaa agtttcacga accctagctg
    21481 tcaaagaatc gatattttat tttaatatag gctattttaa aataaatggt ttaaggtatt
    21541 cctctacaaa agccactcta tcaattatca tgggtttaaa ataagaagtg atggagataa
    21601 ccagattgtt cttagcgtca atataaagaa catttccact attaccaatt gctgaatata
    21661 tttcgttctc agaatctaat acccaccata aatagccata tctcatattt tgtaagtctt
    21721 ctaaagcact ggttctaggt ttgaacatct cgtttatcca catgggtggt acgatttgtt
    21781 ttcctttata catcccttta tttaggcata atacaccaat ttttgccaga tcacttgccg
    21841 ataaacacaa accatatcct gccgtcccga tatttagtgg atcacaaaac cagacattgg
    21901 ttttaggaga cttatccatt gtaaactgct tatgttcttc agctgtttca gcataataat
    21961 tgacatggtt tggtatccct ataggatcaa atagatactt attggcaaaa tcaacagttg
    22021 ttagcttact tgtctgatac aacaagccag ttaatatgtg taaacagaca gtttgatagt
    22081 tgaattcgcc ggttatacct tttcttcctc ccaataaatc tagtgatgtt tttgtccaat
    22141 cttcacttga acagactttt gtccatggat cgcctttaca tttgtagggt gctttcattg
    22201 ttagcaagtg cttgattgta atagcgtaaa tagttttctc cccgcgtttc actttatact
    22261 ctggaaaata atctaatact ttatcatcga tacttttgat taatccttgc tctaaagcaa
    22321 tgccaatcag caaagataca atacttttcg ttgctgacat gatatgacaa gtatcgtctt
    22381 ttttataacc attccattca tctgtataaa cttctacccc atccttatat gcgacaattt
    22441 gacatatatt tggttgtcga aaaccaataa aatcatgcaa ctcttgttta ttcataagat
    22501 attacctcac tttcttctat aagcataatg tcattaaaaa tcgatttcaa ttttttcgat
    22561 tcttcacgta atacaggtat tcttatggac tagacagcgc tttgggaaga tggattctct
    22621 gaaaaatata aaggttcact ttatgagtgg ataaaaaaag cagcaaatac ttcgacttct
    22681 ttaatttacc aacattagtg aaaatcgatt catctggaga tttgtaattt agagtccttg
    22741 tcttgattag aggtgtataa agctttttat tagacatttt taaattccat tacaatttgc
    22801 ttataaacta ggtttttaaa ggtgactatg gtaagataat agagatatta ttttgt
//
LOCUS       DAFEOM010000036        22213 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.037, whole genome shotgun sequence.
ACCESSION   DAFEOM010000036
VERSION     DAFEOM010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.037"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            170..514
                     /locus_tag="LP524_001621"
     CDS             170..514
                     /locus_tag="LP524_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBL2234679.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            515..934
                     /locus_tag="LP524_001622"
     CDS             515..934
                     /locus_tag="LP524_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBL2234680.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1026..1376)
                     /locus_tag="LP524_001623"
     CDS             complement(1026..1376)
                     /locus_tag="LP524_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234681.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1342..2673)
                     /locus_tag="LP524_001624"
     CDS             complement(1342..2673)
                     /locus_tag="LP524_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HBL2234682.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2929..3495
                     /locus_tag="LP524_001625"
     CDS             2929..3495
                     /locus_tag="LP524_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL2234683.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3640..3727
                     /locus_tag="LP524_001626"
     tRNA            3640..3727
                     /locus_tag="LP524_001626"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3672..3674,aa:Ser,seq:cga)
     gene            4061..5035
                     /gene="bsh"
                     /locus_tag="LP524_001627"
     CDS             4061..5035
                     /gene="bsh"
                     /locus_tag="LP524_001627"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBL2234684.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5360..7255
                     /gene="asnB"
                     /locus_tag="LP524_001628"
     CDS             5360..7255
                     /gene="asnB"
                     /locus_tag="LP524_001628"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBL2234685.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEPIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7450..7896)
                     /locus_tag="LP524_001629"
     CDS             complement(7450..7896)
                     /locus_tag="LP524_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBL2234686.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8124..8273
                     /gene="rpmG"
                     /locus_tag="LP524_001630"
     CDS             8124..8273
                     /gene="rpmG"
                     /locus_tag="LP524_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBL2234687.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8361..8879
                     /locus_tag="LP524_001631"
     CDS             8361..8879
                     /locus_tag="LP524_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL2234688.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8956..9912)
                     /locus_tag="LP524_001632"
     CDS             complement(8956..9912)
                     /locus_tag="LP524_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL2234689.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10078..10884
                     /locus_tag="LP524_001633"
     CDS             10078..10884
                     /locus_tag="LP524_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2234690.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10881..11870
                     /locus_tag="LP524_001634"
     CDS             10881..11870
                     /locus_tag="LP524_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBL2234691.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11867..12871
                     /locus_tag="LP524_001635"
     CDS             11867..12871
                     /locus_tag="LP524_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBL2234692.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13003..13545
                     /locus_tag="LP524_001636"
     CDS             13003..13545
                     /locus_tag="LP524_001636"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBL2234693.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13565..14263
                     /locus_tag="LP524_001637"
     CDS             13565..14263
                     /locus_tag="LP524_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBL2234694.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14284..14496
                     /locus_tag="LP524_001638"
     CDS             14284..14496
                     /locus_tag="LP524_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HBL2234695.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14496..15458
                     /locus_tag="LP524_001639"
     CDS             14496..15458
                     /locus_tag="LP524_001639"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HBL2234696.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15476..15871
                     /locus_tag="LP524_001640"
     CDS             15476..15871
                     /locus_tag="LP524_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBL2234697.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16034..16399
                     /locus_tag="LP524_001641"
     CDS             16034..16399
                     /locus_tag="LP524_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HBL2234698.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16396..18207
                     /locus_tag="LP524_001642"
     CDS             16396..18207
                     /locus_tag="LP524_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HBL2234699.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18269..18436)
                     /locus_tag="LP524_001643"
     CDS             complement(18269..18436)
                     /locus_tag="LP524_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HBL2234700.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            18684..19673
                     /gene="galE"
                     /locus_tag="LP524_001644"
     CDS             18684..19673
                     /gene="galE"
                     /locus_tag="LP524_001644"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBL2234701.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19685..21169
                     /gene="galT"
                     /locus_tag="LP524_001645"
     CDS             19685..21169
                     /gene="galT"
                     /locus_tag="LP524_001645"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HBL2234702.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21193..22173
                     /locus_tag="LP524_001646"
     CDS             21193..22173
                     /locus_tag="LP524_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL2234703.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
BASE COUNT         7208 a         3807 c         4619 g         6579 t
ORIGIN      
        1 aaaaaaagcg ttagcaacta tgcttaaaaa gcatatcgac tgaataaact aagcattgga
       61 caggctgaaa gactacattt aaaatgaaat gcgaagaaac aatagaaaat aaagttgcct
      121 tagaaccata gttgaatcac ctaacagtcg tgaagggaga ataagagaaa tggcagaaaa
      181 acaaaccgca ggacgagatc aattaggaga ttttgcacca aaatttgcag aattgaatga
      241 tgatgtttta tttggacaag tatggtccag agaagaatta tctgctcatc agcgtagtat
      301 catcaccata tctgcattaa tttcaggagg aaattttgaa caattgccag ctcatctgaa
      361 gattggcaag caaaatggca ttactagtga agaaatagca gaaatcatta cccatttagc
      421 tttttatgta ggatggccaa aagcttggtc agcttttagt ttagcaaaag aaatctattt
      481 agaagaaaat agcgaagata agaaagaggc gtaaatgacg acaaaacacg aagaaataca
      541 caatggcgtg atttttccag caggtgaaaa aaacgaagct tacgctcaag tatttaccgg
      601 gcaaagctat ctgaatatgt tagtgaatga tcccgaagtt tctgttggtg taggtaatgt
      661 aacttttgaa ccaggttgtc gcaatcactg gcatgtccat aaagacggct atcaaatctt
      721 acttgtgacc ggaggagaag gctggtatca ggaagaagga aagccagcaa gacgattaat
      781 ggctggagat gtcgtcgtaa cgcacaaagg gattaaacat tggcatggtg cgacaaaaga
      841 cagttggttt gaacatttgg cgatcactgc tggtaattca gagtttttag aacctgtaac
      901 agatgaagtg tatgaaggat tggaaaatca ataatgtcaa gcttgaacag ctcctgaaag
      961 tagattttaa tcgaactttc aggagctgct tttttgattc agtcttttat aaatagaaaa
     1021 tagggttaat aataggaatg agaagcacgt cgaaagatct taatattgat aatcggctgg
     1081 gcatacatga tttccacgtc atcaatcaaa ctacctaaca gatgataatc ttcccgatca
     1141 tgtcggggat tacctaatag ttcttgataa tagatttcca tactagggtc ccgaggattg
     1201 ttcagcaaat cagcaagtac atctaaatct tctggctgag tttctgccgt accctgtatc
     1261 tctatgaaaa gcccttcctc agcaaattct actccaatac gtatttcagt tatttcatgg
     1321 gaatataaat aagtaagaag ctcatcgatg attctttttg ataattcttt ttccaagtgt
     1381 ctttttttcc tttctgacag cggtaaagtt atctagtatc ggtacaagga tacctgcaac
     1441 gaaaccagtt gaaaaaccac tgttatatag atttagtcct ccgtgcaaag tgctgacgtt
     1501 atggacaaga gcaatatgta gtacaccagc tattacaccg taaaaactgc cgtaaaaacc
     1561 gcttatgggc gcgagacctg tcccaaacac agctgcaaca agcatgccag tactggtgat
     1621 ctcatgaaaa gttccaaaca aagaagcaat aaatattccg actagtatcg gaaaactgtt
     1681 ttttaaatga caaccaaaag cactgaaacc caccgcagat aaaatagcac cgatgacagg
     1741 tccatttaac tgaccgccaa ccagcaaaac ataagaagac agcatcagac caactaagcc
     1801 tatgttgatc aaagtagcac caataccaga attagcaatg aaatcagttg tcaattttcc
     1861 agatgaatcg aaaatttctc gaatccccaa aagccgaaag gaattataat agaaaccaat
     1921 tccgactgtc agcgaaaaaa agccaaaaat aatccaaatg agaggaaagt gatattcatt
     1981 gaaaaccaat gtattttcga cgattgaata gctgaataac ctcaaaacag cagtaaaagt
     2041 catcgcaatc aacccagagg tgaatccaat attataaagt gtgaatccac gatggaaaac
     2101 taaagtttga gcagctaagg gcggaagaag tagaccaatt gctatgccag ctaaattccc
     2161 caataaaata cctatcagta aaggaaaacg cataccaaaa gtgatataac taacgaccgg
     2221 tgataatgcg ctaccaaaca gtccgatcat gatgtactga gaaaaaggtt tattgacaaa
     2281 tacagcgtat agatacacac ctaaaatgat tgagatagaa ttataaaggt ttttgccaaa
     2341 aaaagagaaa ccggtaactg ttagaatcga agcaaataag ggaccgttta aaatcacctt
     2401 gtgccgataa gctaaaagca cagaaagtaa ggtcaaaatc cctacattta tgaatgcgct
     2461 tccgaaaccg cctaaagcaa aataatctgt caaaagattg cttggcgagg taagaatagt
     2521 tagcaatcca ctccaattat cagtaggagt attagaaagc aagccaataa aaagaaaaaa
     2581 aacacctgta aaaccaagat aaaaatactt tcttttctgt gaaacgattc gtttttgagg
     2641 atgaatataa ataacgtcag tttcgttttc cattacacac cttctttttc attttcttta
     2701 actataactt gaaatgaaag atttggacaa ctatcattta ggtcaaaact aaaaatgtaa
     2761 gggaacccat tgaatttaac caaatttttt taattttgca aaaaaaagtt aaagaagact
     2821 gaaaaaaaat ttaaaatata acgttttgag taaaatgttt gcaaaaaatg ctaaaaatag
     2881 ggtacaataa aacaacatat actgcagtaa agaagtgggg gagttttaat gagaagaaaa
     2941 gtctatacca aggatcaaat tctaaaagct gcttatgatg ttgttgcaaa ggaagggttt
     3001 aaaggattta ctgcgagaaa tattgctaaa aagatgggga tttcgacaca gccgatttat
     3061 ctggaattca aaaacatgga agatttaaaa aatacgttgc tggattctat ctttgaacaa
     3121 ctaaaagaag atgttttccc tattgaacgt actggtgata aaatcattga tctcggtttg
     3181 aactatatct actttgcaaa aaagcatcat agtctttaca tggcattgta cattgatgaa
     3241 catggggacg gaaagaaaat gcatgatttt tcatacaatt atttccgtga aatagttaaa
     3301 gaagatgaaa gatacaatca attatcggat gaatatgtcg atgctctgca tactggtaca
     3361 tgggtagcag tgactggctt agcgtcattg atgtcatcaa acatcatgca tccaacagaa
     3421 gaacaaatca ttgcactgat caaacggaca attgactcga ttttagcatt agataatcct
     3481 gaaataatca gatagtgttc tatcagtatg aatttgatcg gtaagtccgt tggctgcaaa
     3541 ctgtcaatgg acttttttgc taaaaaaagc gatttctgtt atttatctat tagaaatagg
     3601 cttgacattt cgtgaaaaaa gtttaaaaat agtacttacg gaaggttggc agagtggtaa
     3661 tgcaccggac tcgaaatccg gcgaaccggc taataccggc gcgcaggttc aaatcctgta
     3721 ccttccttat atcaacgatt agaggttgtg aagaagctgt cctgcatcaa gtaataagaa
     3781 cggccaaaca aaaatagctt ttcctatttt ttcgtttgat tgggcttatt accgcagatg
     3841 caagcttttg aacaccgttt gttcggaata aggggctgtg acaagacttt tgtcacagcc
     3901 ccttattgtt gtacttttaa cctacaatat tttttgattc cccctgagta ctatttttta
     3961 taaatgctag taaatcaatc ttctttggcc tttttttgat aaaaaaaata ctagatgtaa
     4021 gataaaagtg taaaaacgat ttgttttagg acgggaaacg atgtgtaccg caatcactta
     4081 tgcaacgaaa gaccattatt ttggaaggaa ttttgattat gagatgtctt ataatgaagt
     4141 ggtcactatt actccaagaa attatcgttt cgattttaga aaggtaaaaa atttagataa
     4201 acattatgca atgataggaa tagcagctgg ggtatccaac tatccactct attatgaggc
     4261 tacaaatgaa aaagggttga gtatggcagg actgaatttt cctggcaatg ctgattataa
     4321 agaattacaa gaaggaaaag ataacgttgc accatttgag tttattccat ggatactagg
     4381 gcaatgctca accatagacg aagctaaaga attattggct actatcaatc tagtcaatat
     4441 cgattttagt gaaaaactac ccttgtcacc tttacactgg ttattagctg ataaagaaaa
     4501 atcgatagta attgaaagta tgaaagatgg acttcattta tatgataatc ctgtcggtgt
     4561 gttgacaaat aatccgccat ttgattatca attatttaat ctgaacaatt atcgctcgct
     4621 atcaaatgga acaccagaaa atcatttttc aaaccagatc agcttgaatg tttatagtcg
     4681 tgggatggga ggattgggct tacctggaga tctctcttct gtttctcgat ttgtaaaagc
     4741 cacatttact aaaatgaatg ctgcatcagg tgattcagaa tctgaaagta tcagtcaatt
     4801 ttttcatata ctaggctcag tcgagcaaca aaaaggagta tgcgatactg gagaaagaaa
     4861 atacgaatat acgatttatt cttcttgctg taacgttgat aaagggattt attattatcg
     4921 tacgtatgaa gacagtcaaa ttactgctgt aaatatgaat aaagaagact tggatcgtca
     4981 tgaattggtc agttatccag tcataaaaga gcaacagata aactatgtca attaattaat
     5041 gaatagatcc gtttggtatc atattttctg atacagtcat aattagacac gatctttagg
     5101 tcgtgtcttt ttttgctggt ttttttggaa atagcatcca ctaatatttt tttaacacga
     5161 agagaataag gtagcctcaa aaaagaaaac atggtaaatt agatattggg actttatttt
     5221 ctgtaaaaac agaaactttc atcttttgtt ttcacgtgac gaaattgtaa gagagaaaat
     5281 attcgtccgc tatttattct atgataagat gcatatgaat gaaataaaca gtaattttat
     5341 attaggatgt gaaagttaaa tgtgcggtat tgtaggtttt gttaacgaca aggacaacaa
     5401 aaaaacaatt attaatacaa tgatggatcg aatcgttcac cgtggaccta atagttcagg
     5461 agaatatatc gataaacatg ttgctttagg attcagaaga ttaagtatta ttgacttaga
     5521 gggcggtact cagcctattt acaatgaaga tcggacaaag atcattattt ttaatggcga
     5581 aatctataat taccagcctt tgagagaaga attgattgca gctggccacg tttttcaaac
     5641 acatgcagat acagaagtcc ttttgcatgg ttacgaagaa tggggaacag aattattgca
     5701 aaagatccgc ggaatgtttg cttttgctat ttgggataat gaaaagaatg agttattcgg
     5761 tgcaagagac cactttggga tcaaaccata ttattatgca gaaatgaacg gaacatttat
     5821 gtttggttca gaaatcaaaa gttttctgcc tcatcctgat tttaacaaag aattgaacaa
     5881 agaagcgttg aaaccttata tgactttcca atattctcca ttaaacggcg aaacattctt
     5941 caaagatgtc tatcgtttgc ctgaaggcca ttattatacg tataaagatg gaaaaataga
     6001 cattcagcaa tattgggatg cagattttga aacaaaagaa acgcattccc gacaagaatg
     6061 gatcgaaaaa atcgacgaaa ccgttcaagc ttctattgaa gcacatacgg tcagtgatgt
     6121 agaagttggt tctttcttat ccagcggggt tgattccagt tatgtaacct ctgtattgaa
     6181 accagatcat tctttctcaa tcgggtttga tgataaaacc tataatgaac cgattgaagc
     6241 cagaaaacta accgaactat tggacttaga taatacagca gcagtgatcg acggagacat
     6301 gtcgttcaaa gcattccctt tgatccagta tcacttagat gaacctgatt caaatccttc
     6361 ttgcgtacct ttgtactttt tagcaaatct tgcttcacaa agtgtgcggg tagtccaatc
     6421 tggagaaggg gcggatgaac tgtttgcggg ataccaaacg tatggattcc atacgaattc
     6481 aaaattcatc cgtgtgattg ctcaaggatt gaaaaaatta ccaaaaggaa cacgctataa
     6541 tttaggacgt aaaatcggaa aaatgaaaaa tttccatgga cggatccatt tatatgaatc
     6601 acttgctcct gcaaaagaat attttatcgg tcatgctcga gtgttcgaag aatcagaagc
     6661 atcagaagtg ttaacaccaa aatatcaaac agcaccatct gtggatgaaa tcatgactgt
     6721 tcattacgag aaaacagaag gaatcaaaga tgaagtcaat aagatgcaat atgtcgatct
     6781 tcatcaatgg atgccaaaag atatcttgtt aaaggcagat aaactttcta tggcaagttc
     6841 tttggaagtc cgagtacctt tattggatat cgaagtcatg aaattggctc aacaaatacc
     6901 aagtaagttt ttattgaatc aaaacaacac aaaagatatt ttcagacaag ctgcgaacaa
     6961 acatcttccg gaagaatggt cgaatcgtgt gaaattagga ttccctgtgc caatcaaagc
     7021 ttggttgaaa gaagaacacg gctacgaaca agtcaaagca ttattcgaag cagattttgc
     7081 aaaagaattt tttgatcaag aaaaaatcat gaagttgcta gatgaccatc acgaaggacg
     7141 taaagaagag caaagaaaaa tctggacaat cttcagtttc ttgacatggt ataaagtcta
     7201 ctttgtagat gaaacgattc cgcaagcaga agcaatcgac tatgtgactg tttgatgaaa
     7261 atcaaatatc aataaaaaag aggttttctc ctaccgttta acactgagtg ataagaaaca
     7321 atttcaaaaa acagctcttc ccgttttata gatattatga cttatggttc ggagttggca
     7381 gaggaacacc attgtttcga acataagggg gttgtgacac gattttcgtc acaacccctt
     7441 tacatatgtt caataatcga tttctttttc ttttgtccca tcttttttat aaatgaacaa
     7501 agttgattct ttgttttgtg ctacttgctt tcctttttcg attgcttctt tttttgtatc
     7561 aaaatggttg cttgcctttt ttgccccttt agatttcacg atccattgtt cttcctcata
     7621 ttctacgatc acatctgaat caagcagttt tccagcacga ggattcgtat cgtgttcatc
     7681 cgtcttgctt ggattttttt ctttttcgaa agttcgtttt tctgattcac tagcatcttc
     7741 ataccatttt tttgcttgat caatggcaat tgggatcgca cggttatcag ggtacccttc
     7801 atctaataat gcatttgcga tgtcgattgc tttttttctt gtcaatttat ctaagttttt
     7861 cattgatgca ggaaagtcct tcatgttcca tggcatatta gtatccccct ttattaaaaa
     7921 acataatcta tataaacaag tgtaagggta tttatacaaa aagaaaaaga ttttgtttga
     7981 gaagtaaaac cgttgtattt atcgtcaagt agttttactt aacaaaatcg tgaaaaacac
     8041 tagcaatcta gaaaaaatca tggtaagata atcaagtctg agagtcatct tagaggattc
     8101 ttgtaaaaat gggagggaaa aacatgcgcg taaacatcac tttagagtgt acttcttgta
     8161 aagaacgtaa ctatctaaca agcaaaaaca aacgtaacaa tcctgatcgt ttggaaaaac
     8221 aaaaatattg cccacgcgaa cgcaaagtta ctttgcaccg tgaaacaaaa taatgttttt
     8281 aaagttctca attccttgct ggtcaaagaa ttgagacttt ttttatctca aaacatctaa
     8341 aatcataagg agcggataat atgtttattt ccatgtgggc acaagataaa aatggtctga
     8401 tcggcaaaaa tggtttgtta ccatggcgat tgccaaatga tatgcgtttc tttcgcgaac
     8461 acacaatgga tcgaatttta gtgatgggaa gaaagaccta tgaaggaatg ggggatcttt
     8521 cacttcctta tcgccatatc atcgtgttga caacgcaacc aaattttcgc acgaaagaaa
     8581 atgcggaagt aatgcattcg atcgatgaac ttttgtcgta tagtcaaacg atctcagaag
     8641 atatctatgt ttcgggtggt agtcggattt ttcaagaact tcttccttac actggtcgga
     8701 tctggcgaac attgatcgat agcacgtttg aaggcgatac gtatattggg aatattgatt
     8761 tttcagactt cgcccttata gaggagtatg aggggataac ggatcaagaa aatctatatg
     8821 cccatcgttt tcaaaaatgg gaaagagtaa aaaatagggt tgcttcaaaa gaagaataaa
     8881 atctatgcgt ctagtttgtt aataagaaaa aaagcatctc acgactaatc gtgaagatgc
     8941 tttttttagt taaaatcaat ttagtttttc tttgatatca ttgaccattt cctgactggc
     9001 aataggtccg ttatatagcc aactagattc aggtccgaat tgcagtacat gattgtctgt
     9061 cactgctttt agattcttcc agcttggttc attgaacatg gcagcatctt tgtcactgtt
     9121 cacaaggatg atgtagtcag catctaattc agctagcttt tcagaggaaa cagcagacca
     9181 gtcagaagtg gcttttttgg agacctcttc cactagatca ggaacaccaa attccagatc
     9241 gtcgtatacg attcttccac ttgaccgatt tttgctgacc ataaaggctg agttgttggt
     9301 cacccacaac actgcagctg tcttgttctc gattttatct gctagttctt ctcgagtttt
     9361 cttgatggat ttttgataat cggaaatgat tttttctgcc tctttttctt ttgccaaagc
     9421 tttgccgaca tcttttagtt gttcttccca tgtgacatct gttccgtttt ttacaacata
     9481 agttggcgcg attttgctat attgttcgta tttcccgcct tcgacagtag cggaagaaga
     9541 aatcaacagc aaatcaggct caaagcttaa cactttctca taaggaaggt cataagaaat
     9601 cgttgggaca tcctttaaat catcttgcag ataatcttga atggaaccac tgccaacagt
     9661 ccactgggcg ataggttttt cccctagtgc aatcagatag tcttctaagt aactgccgat
     9721 gatccgtttg ggcgcgtttg gaatctctac tttatgtcca agtgtatctg tgactgtatg
     9781 agtactattg gatgaagtcg tcgaattggc agttgtagct gtttctttat ctgaagaggg
     9841 ggagcaagcg ctcagtactc cagccagcag caatatgcta gataatgtga atacttttgt
     9901 ttttaatttc atttctttcc tcctaacata attgagaatc tttctcattg gttattattc
     9961 ctgatatttc gaacgctgtc aaccgcttca tttggctata gatatgaaag tagcgcttga
    10021 cgaaatgttg aaacagcgtc ataattgaga atgattttca gcgaaaggat gggtgaaatg
    10081 acgagattaa gagcagctga cattgcagcc ggctacgaaa acaaaaaaat cattgaaaac
    10141 atgtctgtta cgattcctga agggaaaatc actagtttga ttgggccaaa tggaagtgga
    10201 aaatctactt tattgaaaag ttttacccgt atactacctg tgaaatcagg aaagatcatt
    10261 gtcaataact ctgatatctc tacatacagt tcaaaagaac ttgcaaaaaa aattgcatta
    10321 ttagcacaaa ctagtgaaca ccctacaggt atgacagtag aagaagtagt atcttacgga
    10381 cgataccctt atcaaaaact gttcagtggc atggacacgc aagatcatga agcaattgag
    10441 tgggcgttgg atgctacagg tctagctgga tttcgtatgc gggatatcgc aacgctgtct
    10501 ggtggacaaa cccagcgtgt ctggatcgct atggcgcttg ctcaggaaac agatatcttg
    10561 atcttggatg aaccgacaac atttttagat ccggcacacc aattagagat cctacagtta
    10621 ttgactgaaa tcaatcaaat gaaacaaaca acgatcttga tgtcgattca tgacattaat
    10681 catgcttcac gtttttcaga ttatttatta gcaatgaaaa acggtaagtt gatcacagaa
    10741 ggtacacctg aagaagtgat cacaaaagag tgtatgaaaa aaatatttga gattgatgca
    10801 acgattgtca cgcttccaag aacaaataaa ccagtttttc taacctatga tttgttgcca
    10861 aaagagcagg aggatcaatt atgaggacta tgaagaaaaa tagactcctg cttttggtct
    10921 gttttgttct tttgattatt ttttttgttg ctctttcaaa tggcgcagcg actgtgggaa
    10981 aagcagattt ttggcaggcg ttgtttgatt ttgatagatc aaaacaaagt caccaaatca
    11041 tacgaaatat tcgtttgcct cgtgtggcgg gagcatttct tgtagggagt tgttttgcat
    11101 tgagcggtgc attgatgcaa ggagtgacaa gaaatccttt agcagattca ggcttactag
    11161 gaatcaacgc gggtgcttcg ttggcaatgg cccttagttt tgctttttgg cctaaagctt
    11221 ctgatttttc tgtgtttcta atgtcattgg caggttcttt tgctgtaact ttgctcgtat
    11281 ttggtttttt gcagttttcg aaaatgggca tgaatccttt acagctgatt ttagctggcg
    11341 tagctattag ttcttttttt actgctatca gccaagcact tacccaattc tttaatctgc
    11401 agcaggatct tgctttttgg tttgtaggcg gagctgccaa tatcacatgg aaacaattag
    11461 cagtattgtt tcctttatat atacttgctg ttttggtttc tttttcatta ggaaaagcaa
    11521 tatctttgat tcatttgagt gaaacacatg cattagctct tggaagtcat cctaaacgaa
    11581 cgcggttgat tgttttctgt ctagttgcag tgttagcagc cattgccgtt tcgttagtcg
    11641 gacctgtttc atttattggg ctgttggttc cgccagttgt tcgcggaatg gttggaacgg
    11701 actatcgtta tgttctacct gtctcttttt tagttggcgg tggattggtt atgttggcgg
    11761 attttacagc gaggatgatc aatccccctt ttgaaacacc ttttggatta gtcattgcat
    11821 tgattggcgt tccgttttta ctgatacaag taaggaggga acaacggtga aaaaaatata
    11881 ttggacgttc tatttcttgc tttttgtact tgtcatcctg tctataggta gtttaatgat
    11941 tggtacgcct tttatttacc tggatcagct ttttgccacg ttcataggga aagaatcatc
    12001 ttctttggcg ctgattgtta gagaatttcg agttcctcga ttactgatca gtttattagc
    12061 aggagcttgt cttggcgttt ctggttatat cttgcaagga gtaacaagaa atgaattggc
    12121 agattctagt atattgggca ttaatgccgg agcaggattt ttagtgatgc tttatttagg
    12181 tttcttttcg cactattcgc tgccgtttct attgctagtt gtagcctttc taggtggtgt
    12241 tttagctgct tgctgtgtct atcttgcggc ctatgatcga aatggctttt tgggtatgaa
    12301 tcgaatctta ttgtccggta ttgccgttaa tgccggcctt tctgcattaa cattgctatg
    12361 cacgatccaa ttatctaagg aaaattatgg atttgtgaat gcatggttag cgggatcaat
    12421 ctggggtgcg agctggcctt atgtttgggc attgcttcca tgggcggtta tcttaattcc
    12481 gctaggagga ctttatgcac agaggattgt attgctatca tttggtcaag aacgcgcgca
    12541 aggattaggc cttaacgtgg ctaaaagtca aaaaatcctt ttgttgttag ctgttgcttt
    12601 ggcaagcggt agtgtagcgg tgacaggtag tttatctttt gtaggactac tagctcctca
    12661 tgctgcgaag ctagtcgtac gtaaagaaaa taactggacc tttgttctga gcggtttgtt
    12721 cggttccatt tttctgctta gtgcagatat ggttgctaga gtgattctac taagtgggga
    12781 agtacctaca ggaattttga ttgccctttt tggtgcgcca tattttctgt atgcactttt
    12841 tatcagacaa aaacacgcgc tgaacagttg aaattcatta aaaaaggaag gccaatcatt
    12901 tctaggtctt ctttttattc tatttatgca tttatttgaa agttcgattg ctatttttct
    12961 ttttttccta taccatttta tgagagaagg aagggtgagt ttttggacca attgaaagta
    13021 agattacgcc ggaaaggaat cgaaaagctg aaaaatctgg ccaaaagtgg caagaaaaag
    13081 gaaaaagaac agcgaatcta taaacagcta ttcgccagcg agtactggaa gtcagcaaaa
    13141 gtgataggga cgactttatc gaatgaattt gaactggata cacagccggt tatccaacga
    13201 gcagaaaaag aacaaaagaa aatagttgta cctaaaactt tgccgaaacg tcagatggcg
    13261 ttttatgaaa tggatgaaca gtccgttctt gtacgttcga gttttggtgt tcttgagcca
    13321 caatcagaga agctctattt gccggagatg atcgatctcc tcattgtacc aggtattatc
    13381 tacaatagag ccggttatcg aatcggtttt ggcggtggtt attacgaccg gtatttaaat
    13441 aattttcata aaaaaacatg tagccttgtt tttcgtgaac aattgaatga acaatggcaa
    13501 ccggagagct ttgatcagaa gatccaacaa ttgtttattg actaaaatga aatgggaggt
    13561 tttcatgaat tatcaacagc aaataaaatt gcgtgcatgg ttaagacgac cgttatggac
    13621 ctatgctttt ttagggatac agacagcggt cttcctcatg atggaattat ttccgcgact
    13681 tgagatccct tattatgccg gaatgtatgg tccttatctt gttcacttca atgaatggtg
    13741 gcgtttagtg acaccaatct tcatccattt tggactgatg catttcgtaa tgaactcact
    13801 gatactgtat tttatggggc agcaaatcga ggcaatctat ggccattggc gttttttctt
    13861 gatttatatg ttcagcggga taatgggaaa tacagcaagc tttgctttta acgaagcgaa
    13921 tgttctgtct ggtggtgcaa gtacttcgat attcggtttg ttcggtgcgt tgtttatctt
    13981 agggtttcat tttaaacata acccagcgat ccagcagcta gtcagacatt ttcttttatt
    14041 tattgtgttg acatttgttt ttggtcttct ggatacctct gtagatgttt ggggacatgt
    14101 cggtgggcta attggcggat tagtgttagg gaatgttttg ggtctaccga aacaacagac
    14161 aagctattcg atacaccaaa ggattctttc tacactagtt tttgtatttc tttttgtaat
    14221 atgtatttta ctgggtttaa aaaaatatgg tttacttgta taatatgaag gaagtgctta
    14281 aagatggaga gcttatatga tgtacagcag ctgttcaaaa aatttggcat ctttatctat
    14341 gtgggtgcac gcatctatga cattgaatta atgactatcg aattgcgtaa tctgtatgat
    14401 agccgtttga ttgatcatga tacatacatg acagcgtggc gcatattaaa aagagagcac
    14461 cgaattgaag aaagccgaaa gaaaggaaat ttgtaatgga caagaagatt attgggattg
    14521 atttaggtgg aacaacagca aaatttgcaa ttttaacacc agaaggagag attcagcaaa
    14581 aatggagcat cgataccaat attttggatg atggtaagca tatcgttcct gaaatcattg
    14641 aatcaatcaa tcatcgtttg aatttatata atatgaaagc cgaagacttt atcggaatcg
    14701 gcatgggtac cccaggaagc gtggacagcg aagctggaac ggttattggt gcgtataact
    14761 taaactggac agaagttcaa tttgtgaaaa aactgatcga ggcaggaaca ggaatcaaat
    14821 tcgctatcga taatgacgcg aatgttgctg ctttaggtga acgatggaaa ggtgctggag
    14881 aaaacgatcc agacgttgta ttcgttactc taggaacagg tgttggcgga ggtatcgttg
    14941 ctggcggaaa tttgattcac ggcgtgagcg gagctggagg agaaatcgga catatcactg
    15001 tggatcctga agggttcgaa tgtacttgcg gtaaacgcgg atgtttagaa actgtatcta
    15061 gcgctacagg tgtggtacgc ctaggtcgtt tcttggcaga agaatacgca ggggattcaa
    15121 aactgaaagc aatgctagac aacggagaag aagtaacaag taaagatatc tttgaaatgg
    15181 ctcaagaaga cgatccgttt gcattgatgg ttgttgatcg cgtatgcttc tacttaggtt
    15241 tagcttgcgg aaacttgggg aatacattaa atccgtcaag tatcgtgtta ggtggaggag
    15301 tatcggcagc aggagaattt ttacgtagcc gtgtagagaa atattttaaa gaatttactt
    15361 tcccgcaagt aagagaatca acaaaaatca aattagcaga gcttggtaat gaagctggtg
    15421 tcattggtgc tgcctcatta gccttgaaat ttgcttaaaa aaacggaggc attacatggt
    15481 tttatgggta attaatatca ttttgctatt gattgtgctt gcaatcatcc tttgggaagt
    15541 ctatctacga gtaatggcca aacgttcagc aacgactttg acagaagaag aatttcgaga
    15601 aggaatgaga aaagcacaag taatcgatgt tcgcgaaaaa gatgtgtttg atgctgggca
    15661 tattttaggt gcaagaaaca tcccttacac tgttttgaaa gattcattag gatctattcg
    15721 aaaagaccag cctgtctata tttacgatca aaagaaaagt ttgagcatcc gtacagcaaa
    15781 taaattacgt aaagcaggtt atcaagagat ttatttactt aagggtggat atgacggatg
    15841 gtctggaaaa atcaaaagta aaaaagtata aaacagatga ttgtatgaca gctttaaaaa
    15901 ccgaatcaat cattagattt tgaccaagat cacatgaacg gcgcaatgga tagttgtgcc
    15961 gttcattttt tttattttgt gctattatct acatatataa gctaagcgaa aaggtagaaa
    16021 gaaggaaaca actatgatca aattaaaacg tgtttacctt gatgctgaaa aagaggatgg
    16081 ctatagaata ctcgtggata gattctggcc acgaggaatt tcaaaagaaa aagcgcagtt
    16141 agatctgtgg ttgaaagaaa ttgcacctag taatgaagca cgcaaaactt ttgaccatgt
    16201 accagaacga tttgcttcat ttgcaaagaa ataccagcaa gaattgattt tcgaagaaga
    16261 aaagaagcaa gctattttta aactcagtca acttatgaaa gaacatccag tcattacttt
    16321 tgtttatggt gcaaaaaatg aacatcagaa ccatgctgtt ttattgaaaa aatttatcga
    16381 aatggaggca tgcaaatgaa aatagggatc attggtgccg gacctagagg attaagtgta
    16441 gcagaaagga ttctccataa taatcaggac agacattctc ttgaactttt tcttttcgat
    16501 cccaatggtc ctggaggtcg agtttggcgg ttgaatcagc caccggagtt attaatgaat
    16561 tctgtttctc aacaagtgac gctttttaca gatgaaacat tgtccagtgg aggattggta
    16621 acacagggac ccaacttgtt tcaatggagt aaaggacctg ctacagatta tatcgaacag
    16681 acggtaacca aaaataagca actaattata gaagaaatca atcgtctgga aaaagatcag
    16741 caatctactc gatgtttata cggactctat caacgctggt ttttctcaaa gttgcaagaa
    16801 gaatttccag cttctattca gtttgtttat tctttagtaa aaaaagtaag caaaagaaaa
    16861 gatggttatc ttttacagac agatcagcaa agctattcta tcgatcagct tgttttagca
    16921 agcggtcact gggaaaatga tgagacagaa gaagaaagag tgtgggtaga ctacgcaaag
    16981 aaaaatgttt tactgtatca gcttccttct aatcctgcag atgtagatct ttcgaagata
    17041 ccgcctaaag aaaatgtctt tttaagaggt ctaggcttaa gtttttttga ttatgtcgga
    17101 ttgtttacag tatcaagagg agggcgcttc gagaacgaaa agggaaaact ggtctatcat
    17161 ccatcaggca aggaaccgat tgtatacagt ggatcaagaa gggggctccc ttattaccct
    17221 cgaggaagaa atcaaaagca aggcggagca atggcatggc cacggttact aacaaaagaa
    17281 aacctggagc aatggcatca gtcgggattt ttgactggcg aaacattttt tgactatctg
    17341 aaaaaagacg ttgagttgtt ttattacaag aaaatcatcg aagaatatca cttgccgatt
    17401 ccaaagaaaa attttgagca tgattttctc tccctctctt cagaggagtg tctcggaaag
    17461 catccagcac taatccctta tagatgggat tggtcgtatt tagaaactcc tcttgcctat
    17521 cagaatgagc ccttttcaga agctagccga atatttattg aagggcagat aaaagaagcg
    17581 gaaaaaggca attgcacagg agcatttgct tcaacatttg atgcattaaa agactggcgt
    17641 gatccaatcc ggcaggctat tgaatggcac gttttttctg ctaaggaata caaagaaata
    17701 ctatggggat ggtttactca tctaaatagc tttttaacga tcggtccgcc aatcattcgg
    17761 acgaaagaat tagcagcact gatagatgcg ggtatttttc atttgatcga accgccattt
    17821 gatattcagc aaaagcacaa ccgctatgtt atttctctac aagataaaga aatcagcagt
    17881 cgctatttga ttgaagctcg attgccacaa acaagctttt ctcgttcaag taatcctgtc
    17941 ataaaaaatt tgaaggaaaa caaacttgtt cgcccttttt cattttcaga taatacagga
    18001 agttatcatt cagaagctat agatgttgag cgaggtacga atcgcgtgat tgatgcttca
    18061 ggggaaatcc agtctgactt ttattgctat ggaatacctg ttgaaggtgt ggattggcta
    18121 actgcagcag tagctagacc ttatacagat ccatggaatc tgagacaggc agataaaata
    18181 gcacaactta tattgactga caaataaaaa tacgcatgat gacttatctg ttttttagaa
    18241 agtcttcatg cgtattttta aataattatt aacgagaaac gcgtttacgc attgcttttt
    18301 tacggttttc ttcgatcatt gcttcttttt cgtcaattgg atcgatcact gtttttttca
    18361 cagaagcaac caaaccagca acggcagcgg ctgttgccag acttcctact aatattccag
    18421 atacaaattt tttcattgaa tccacctcat ttattatttg ttatatctat tatggaacaa
    18481 aagagaaaaa aaggctagtt ttacgtttgt ttgtaaacct ttgcaaaaaa tccttgaaaa
    18541 tgtttactaa atgttttatt ataaatttac catatgaatt tacgaaattt tactttgttt
    18601 gttttcacca taaaagtaaa cttcgttttt taaattatat agaatcaaca aacacaattt
    18661 tactaatcaa aggagtgttt tttatgacaa ttttagtact aggcggagcc ggttatatcg
    18721 gttcgcacgc tgtagaccag ctagttcaaa aggggtatca agtagctgtg gtagataatt
    18781 tgctcacagg tcacaaacaa gcagtacatc cagatgctca tttttatgaa ggagacatcc
    18841 gggataaaga atttctacgt tccgtatttg aaaaggaacc gattgaagga gtaatccatt
    18901 ttgctgcaag ctctttagta ggagaatctg tcgaaaaacc attgatgtat ttcaacaaca
    18961 acgtatacgg tatgcaaatc ttacttgaag tcatgcacga gttcaacgtg aataaaatcg
    19021 tattctcttc tactgcagca acttatggag agccgaaaga gtctccaatc acagaagata
    19081 caccagctaa tcctaaaaat ccgtatggtg aaagtaaatt gatgatggaa aaaatgatga
    19141 agtggtgtga ccaagcatat ggcatgcgct atgtcgcttt gcgctacttc aatgtagccg
    19201 gagcaaaagc agatgcatct atcggagaag atcatacacc agaaacgcat cttgtcccaa
    19261 tcattttaca agtagctctt ggtcaaagaa aagccttggc tgtttatgga gacgattatg
    19321 atacaccaga tggaacatgt atcagagatt atgttcaagt agaagatttg attgctgcac
    19381 atattttagc tttggaatac ttgaaagaag gaaatgaaag caacttcttc aatttaggca
    19441 gcagcaaagg atactcagtc aaggaaatgc tagaagcagc tagagaagtg acaggaaaag
    19501 aaattccagc agaaatagca ccaagaagag ctggagatcc tagccgatta gtcgcttcaa
    19561 gtgaaaaagc gagagagatt ctgggctgga aacctgaata cacagatatc aaagcgatta
    19621 tcaaaacagc atgggattgg catgtcagcc atccaaatgg ttatgaagaa taggggaatg
    19681 agatatgtca atcagtcaaa cgatttgtga ttttgccaca cttgcgatcg aagctggcgg
    19741 atggatggaa atggaccgct tatatttaca gaatcgtctt ttagggatgg taggagaaga
    19801 tgctctagaa agcattgaag taagaccagt taaggaagaa tctgtagact tattggatca
    19861 gcttgtcgct aaggcaagag aaaatggtgt tctttcagaa ttgccagcag aacaagagat
    19921 ttttgaagcg caattaatgg attttctcac accgccacct tcagtcgtca atgcattctt
    19981 tgcacagcac tattcgaaaa gcccagagga agcaactgag tatttcttta atttaagtaa
    20041 gaaaaatgac tacatcaaaa caagagccat cgcaaaaaac atcaaatttc cggcagagtc
    20101 ggagtatggt gaactggaaa tcacaatcaa tttatctaag ccagaaaaag atccaaaaca
    20161 aatcgcagca gaacgcgagg cggtaaaagt agactatcct acctgcatgc tttgcatgga
    20221 aaacgaagga tatagaggtc gcttgaacta tccagcccgc accaatcacc ggattattcg
    20281 aatgaatcta gacggtgaaa gctggggatt ccaatattcc ccttatgctt attacaatga
    20341 acattcgatc attttatcag aacaacaccg cccgatgaaa atcacaaagg aaacatttgc
    20401 tagactgctt cggattactg aagtcttgcc tcattacttt gttggttcga atgctgactt
    20461 accaattgtt ggaggatcga ttctttctca tgatcattat caggcaggac gtcatgaatt
    20521 tcctatggca aaagcaccga tagaaaaact tatcaaacta gaagattatc cgcaagtcat
    20581 cgcaggagtc gtgaaatggc cgatgtctgt gatccgtttg caatcaacag agaaaaactt
    20641 gcttattgaa gcagctaact cagtattgga aaaatggaaa gcatattcgg atgaaacagt
    20701 atctatcaga gctttttcag cagatggtac accacaccat acgattacgc caattgcacg
    20761 aagaagggac aattactttg agttggacct tgtccttcga gacaacaatg tttcagaaga
    20821 acatcctgat gggatttttc atccacataa agatgtccaa catatcaaaa aggagaacat
    20881 tgggttgatc gaagtaatgg ggcttgctgt tttaccacct cgtttgaaac cagaactagt
    20941 agaagtagag catttcttat taggagaaga aaaccagatg gctgaatatc atcgtccgtg
    21001 ggcagaacaa ttaaaaaaag aacatcctga tttgacgaaa gatacggtca ccgatgtcgt
    21061 acaaaaatct gtaggtcagg tttttgcacg tgtactggaa gacgctggag tattcaagcg
    21121 agatgaaaaa ggacaggcag cgtttgaacg atttactgcg attttataaa cgtaaaaaag
    21181 gaggagagac aaatggcaac aattaaagac attgcgcagt tggcaggagt ttctcctgct
    21241 actgtttcaa gagttctcaa ctatgatcct gatctttccg ttggcttagc cacaaagcaa
    21301 aaaatttttg aagcagcaga acgcttaaat tatacgaaac atcgtaaaaa tgcagcaaat
    21361 actaagcaaa cgcttcgcct gatccagtgg tacgacgata cggaagagct tgaagatctt
    21421 tattatttat ctattcgcct aggaatcgaa aaaaaggcag aagaagcaaa cgttacttta
    21481 ttgaaagaaa cgtgggattc cattagtgat cttccggcag atggaacgat cgcactagga
    21541 aaattcgacg aagctcagtt attagaaatg aaaggatccc aaaatgcctt gcttttggta
    21601 gattctgacg gcacttctca aggaatcgac tctctagttg tcgactttaa gtcaagtgtt
    21661 caaaaagtca ttgaacactt tgtctcccat aaagcgcaac atatcgctat gcttgctgga
    21721 acagaatata cgaagaaaaa ccatcagcgg ctaaaagatc ccagaattat ggcatttcaa
    21781 caagttttgg cagaaaaagg ctacgacgaa ggtccaatta tcgaagcaga tttttctgtc
    21841 gagtcaggat atcaagcagt gaagcagtat ttaaaaacga atccgcatgt tcctgatgcg
    21901 ctttttgcag ctaatgatgc attagcgatt ggcgcactga aagcgattca agaaaaaggt
    21961 ttagtggtac cagaagatat ttctgtgatg ggcttcaatg atgtcagtgt agcgaaatat
    22021 gtaacgccct cgctttctac tgtaaaagtt tataccgaat ggatgggaga gctagcagtg
    22081 gagacgattc ttcaattaag taattccaaa gcaccggttt caagaaagat tattgtggaa
    22141 acagagttga ttattcgaga atcaacgaaa taaaagatgg aaaagctgtg ggaaaatgga
    22201 gtgataccgc agc
//
LOCUS       DAFEOM010000037        22132 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.038, whole genome shotgun sequence.
ACCESSION   DAFEOM010000037
VERSION     DAFEOM010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.038"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            <1..523
                     /locus_tag="LP524_001647"
     CDS             <1..523
                     /locus_tag="LP524_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBL2234704.1"
                     /translation="GGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGIVA
                     NCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIFGS
                     LIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILTVL
                     LLPLFFQPFAGLF"
     gene            523..2109
                     /locus_tag="LP524_001648"
     CDS             523..2109
                     /locus_tag="LP524_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2234705.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            2128..2349
                     /locus_tag="LP524_001649"
     CDS             2128..2349
                     /locus_tag="LP524_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HBL2234706.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            2346..3107
                     /locus_tag="LP524_001650"
     CDS             2346..3107
                     /locus_tag="LP524_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HBL2234707.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            3487..3996
                     /locus_tag="LP524_001651"
     CDS             3487..3996
                     /locus_tag="LP524_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBL2234708.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(4071..4610)
                     /locus_tag="LP524_001652"
     CDS             complement(4071..4610)
                     /locus_tag="LP524_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBL2234709.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(4750..5361)
                     /gene="rpsD"
                     /locus_tag="LP524_001653"
     CDS             complement(4750..5361)
                     /gene="rpsD"
                     /locus_tag="LP524_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBL2234710.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            5623..6474
                     /locus_tag="LP524_001654"
     CDS             5623..6474
                     /locus_tag="LP524_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBL2234711.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            6502..7137
                     /locus_tag="LP524_001655"
     CDS             6502..7137
                     /locus_tag="LP524_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBL2234712.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            7157..7930
                     /locus_tag="LP524_001656"
     CDS             7157..7930
                     /locus_tag="LP524_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL2234713.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            8338..9135
                     /locus_tag="LP524_001657"
     CDS             8338..9135
                     /locus_tag="LP524_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HBL2234714.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            9113..9526
                     /locus_tag="LP524_001658"
     CDS             9113..9526
                     /locus_tag="LP524_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBL2234715.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            9510..12155
                     /gene="mutS"
                     /locus_tag="LP524_001659"
     CDS             9510..12155
                     /gene="mutS"
                     /locus_tag="LP524_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBL2234716.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            12173..14281
                     /gene="mutL"
                     /locus_tag="LP524_001660"
     CDS             12173..14281
                     /gene="mutL"
                     /locus_tag="LP524_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBL2234717.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSKLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            14303..14863
                     /gene="maf"
                     /locus_tag="LP524_001661"
     CDS             14303..14863
                     /gene="maf"
                     /locus_tag="LP524_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBL2234718.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(14948..16936)
                     /gene="treP"
                     /locus_tag="LP524_001662"
     CDS             complement(14948..16936)
                     /gene="treP"
                     /locus_tag="LP524_001662"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HBL2234719.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            17159..17872
                     /gene="treR"
                     /locus_tag="LP524_001663"
     CDS             17159..17872
                     /gene="treR"
                     /locus_tag="LP524_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HBL2234720.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            17949..18395
                     /gene="msrB"
                     /locus_tag="LP524_001664"
     CDS             17949..18395
                     /gene="msrB"
                     /locus_tag="LP524_001664"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBL2234721.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            18565..19167
                     /gene="ruvA"
                     /locus_tag="LP524_001665"
     CDS             18565..19167
                     /gene="ruvA"
                     /locus_tag="LP524_001665"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBL2234722.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            19180..20181
                     /gene="ruvB"
                     /locus_tag="LP524_001666"
     CDS             19180..20181
                     /gene="ruvB"
                     /locus_tag="LP524_001666"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBL2234723.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            20210..20788
                     /locus_tag="LP524_001667"
     CDS             20210..20788
                     /locus_tag="LP524_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL2234724.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            20840..21322
                     /locus_tag="LP524_001668"
     CDS             20840..21322
                     /locus_tag="LP524_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HBL2234725.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            21439..21813
                     /locus_tag="LP524_001669"
     CDS             21439..21813
                     /locus_tag="LP524_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234726.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7237 a         3991 c         4791 g         6113 t
ORIGIN      
        1 cgggggcaat tttttgacta tgctgattac tcctgcgaca gtggcacttg ccatccctct
       61 ttataaaaac ttccgtttac tgaaagagaa tttttatcct gtaatggctg cgattttagc
      121 aggaatagtc gcaaattgct tggttagtgt agggttcggt tacttcttct ctcttcataa
      181 agagatggtg atctcattac tacctaaatc tgtaacgacg gctatttctg tcgatttatc
      241 tcatacgatg ggcggggtga atgccgtgac acttgctatt gttgtctcta caggtatttt
      301 tggttcgctg atcgccactc atattttccg cctattcaag atcgaaagtc cagttgctcg
      361 cggtgtggcg ttaggaagta cttctcatgc tattggtaca gcaaaagcaa tcgagattgg
      421 tgaaatagaa ggaatcattt ccggacttgc gatatgcgtc aacggaatat tgaccgtttt
      481 attgctaccg ttattctttc agccttttgc tggcttgttt tgatgcgaaa accactattt
      541 tatgctacaa taaccaaaac gttcgagcat aaaaaaggag ggaaagacgt gtatttgtct
      601 tccgagtatc ttaaacaatt tgataaagat ctatattata agattctcct cctagaaagt
      661 tttgaggatc aagcttggca taccgctgca caattagcgc aagttgttca gctggatgcc
      721 cgcagcgtgt cgaaatacct gaacgagctg tctaaaaact atcagcagtt tagcggtaag
      781 acacaccctt tgtttacaaa aaatcaccgt tctggttata acttttatga tacgctcgat
      841 tcaattgaac atgagcgttt tttgatctat ctcgtgcaaa gtacgctgaa attccagttg
      901 ctgcacgaca ttttttttga agaattccat acaatgtacc aatttgcgca aaagcattac
      961 attagtgaat ctactgctca tcgaaaaatc aatgaatgga aacagcaact gcaaacttac
     1021 ggaattcggc tgcaacgagg tacatatatc gctcaaggcg aagaagaaat cattcgactt
     1081 tacttgcata tgacattctg gcagttgttt cgagggaaaa tctggccatt tgaaacgatt
     1141 tctcaaatgg acgtaaagaa tatggcagaa catatcatgg ctttttttaa tgttcgtctg
     1201 aacgagatca aaaaaagacg attggaatat atgctaggtg cctttttttt aagaaaatca
     1261 cagaagcatt atgttgtatt gaatgagaaa aaaagacggc tgatttctga caatctgttg
     1321 ttccagcgat tttgccaggt catggagccg gtatttccta attattttca agtagaagac
     1381 gagttaggcg cattatttct tgtactgatg actcgtgaag aatattatag tgatcctaag
     1441 atacggaaga aaatttttga ttttcatcaa caagcaaaaa caccgccctt tacagccctt
     1501 tcagaagcaa aagccgctct ttctctttat caggaagagc aggggctgcc agcagaaaat
     1561 ctaaccttcg aggcagaaaa ctatttattc agcagtcatt ttttcgccta tctttttcct
     1621 aatgcgaaag aaacgattga tggcaatagt agtgatttta ttaatcattt agttattgaa
     1681 aacaaggaat tgaaacaatg gctcgtacat ttttttgaaa gtagacacaa acatcccaat
     1741 catctggcat ttaaaaatca tgcgtttttg atggggcggt atctgactgt gtttaaaaca
     1801 ttaggagcct ttactccaca gcttccaaag atcacgatcc ttttaatgac agatttccca
     1861 ttatttgaag agcaactgtt agaggaggga ttacggacat ttttccgtaa tgagtatcag
     1921 ctgatttttc tgccaacaga ttatcggggg agagaagttg atcttctgat ttcgacaagt
     1981 aaagtccacc gcaaaccatg ggcggatctt gactatttta ttgtgactga agaactaaaa
     2041 ttaattgatt acatccaact atctcaaaaa tttgaaatga ttcaaaaaca aaaactatta
     2101 aaacaataac aaagaaggag aaaaaagatg acatttgaag aaattttacc agaattgaaa
     2161 aaaggtgcaa agattattcg taaaggatgg agtggatttg aactttatgt tgttctcgta
     2221 tccgatgatg aatttgatgg ctgtccggtg acgccgtact tattgatcaa aacaagtgac
     2281 gaaggttttt ccagcttcgc tcctacagtt tgtgatattt tagcagatga ttgggcgatc
     2341 gtagaatgaa aaaccagtat ccagatctag ctaaacaggt tgtatttatc acaggagctg
     2401 cttctggaat tggtgctgcg caagcccgag cgtttcttga acaagatgct tttgtatttg
     2461 gattagatca aaagttcggt aaaatggaag aaatttcttt gcgatttcct gaaacttttg
     2521 cctacgcact aggtgatgtt cgacaaatga aagaactcca acaagcggta tcgaaatgcc
     2581 aaaatcattt tggtgaagtt acgattttac tgaatacagc ggggatattg gatgcctaca
     2641 aacccttgat ggaaactgac gaatcgctgt gggatctgat ttatgagacg aatgtgaaaa
     2701 gcatgtatca gctgacaaaa atcatccttc ctgatatgat ccgccaaaaa agcggaacga
     2761 tcatcaatat ggcttcgatt gctggcttga ttgctggtgg aggtgggatc gcttatacaa
     2821 gcgcgaaaca tgcgattgtc ggatttacca aacaactcgc tctggattac gcaggacagg
     2881 gtatccgagt aaaaggaatt gctccaggag ccatccaaac accaatgaat gcggctgatt
     2941 ttgaagggaa aggtgagatg gcagagtggg tggcaaatga aacaccagtc aagcgatggg
     3001 cacagccgga agaagtagct gagctgacgt tatttcttgc ctctcctcaa gcaagctaca
     3061 tacaaggaac gatcatccca atcgacggcg gatggttgtt aaaataacct tgtgtttttt
     3121 tcaagcttct gtatgataaa actaaagaga gaaacaacgg ttgatgataa aaaaagtcga
     3181 tgactgttcc cgacattcga tgccgaacag ccagttcaaa aaattctttt taataatcgt
     3241 taatattcga aactaagcgg cttttcacga cctcttgata taaacgatat tcaaaagctt
     3301 gcaactgcgg taacaagtcc agccaaatga aaaatataag gagctatttt tatttgacca
     3361 ttcttattac ttgttgcagg gcagcttttt cactaccttc tgcgacactt cagttggaat
     3421 tttcttccca tcctttggtc gcggtcatta ccaagataaa attatcttgc aaaggagaaa
     3481 tagaacatgg aaaaaaataa tacccgttca gccaaacgct ggacaacgac agatgtgact
     3541 cggatggcgc tagtcactgg tttatatgtg gcagtgacgc tggttttgtc tgtctttagc
     3601 tttggagtga ttcagattcg tctctcggag atgttcaact atctgtcctt gtataacaaa
     3661 agatacatcg tcgcggtaac agcgggagta gctttggcta atattgcttc tcctcttgga
     3721 ctgatagatg ttgcagtagg aagcgtcagc acgctgcttg tattgcttat caactataaa
     3781 atcactagtc gcatcaaaaa catgaaaatc aaaatggccg tgacagcatg cgtatttgct
     3841 ttttccatgt ttacagtagc tggtcagcta acgattttat atcaactacc ttttttcttg
     3901 aattggtggg tgattgcttt aggagaattg gcatcaatga ttgtcggtgg gataattatt
     3961 tattggatcg gtaaaaaagt ggatttgtca aaataaagat ctgtttaatt taaaataggg
     4021 cagcatggaa gagactgaat gttgagtctt cttccatgct gcttttttga ttaatttgta
     4081 tgaagaaatc gatttagccg ttttttcatc aagataccga tataagctgc tccagttgcc
     4141 ttgattgctt ccggcagtaa aaacggcagt agtccactgg caaatgcagt tgtaaaagta
     4201 agatcaccac ttattttcag ccagatcgtt ccgcagatca atgcagcaag agagcccatt
     4261 agattggcaa agattgcata taagtaatga gcatccgttc tttcgatcat ccaaccagtt
     4321 atccacgttt gtaagagaaa tccaatcaaa tatccgccag ttggcccaaa taaaacagct
     4381 atacctccag tcatccctga aaatacagga agaccaatca agccaagtaa aagataaact
     4441 ccaacagata tcaatcctag ttttcttccg agaaaagttg ctgttaatcc aatagcaaat
     4501 gtttgccctg tcaaaggaac caaaccaaaa ggaatggtca tctgagaaag gacagaaata
     4561 atcgcggcaa attccgcagc aagtatcaat tctttcaatg ttcggttcat aacgccctct
     4621 tttcgttaac ttaatatttt tatttggtta acgaaattgt aaaacaaagt gattactttg
     4681 taaatagaaa atataaaaaa agaccagcta tccaatagat agcggtcttt ttcaagaaag
     4741 cttttgaaat tataagcttt ggttgtagta ttcaacgaca agagcttcgt cgatttctgg
     4801 atatagttcg tcacgttctg gtaaacgagt caatgaacct tccaattttt cagcgtcaaa
     4861 gcttacgaat gctgggcgtc caacagttgc ttctacagct tcttttactg ttacgatgtt
     4921 ttgtgatttt tcacgaactg agatcacttg acccacttct acgtggtatg aagggatatc
     4981 aacgcgtttg ccatcaacta aaatgtgacc atggttaact aattgacgtg cttgacgacg
     5041 agtcgttgca agtcctaaac ggtaaacaac attgtctaaa cgttgttcta gtaagatcat
     5101 gaagttaaca ccatgtttac cttctttgat tttgctagct tttacgaaca agttacggaa
     5161 ttgacgttcg ttcattccgt acatgtgacg aagtttttgt ttttcagtta attgcatgcc
     5221 gtattctgat tgtttgccac ggctattagg tccatgttga cctggtgcgt aagggcgacg
     5281 tgctaattct tttcctgtgc ctgacaatga gatgcctaag cgacgagaaa ttttccatga
     5341 tggtcctgta taacgagaca ttaaaatttc ctccaatgaa tataagtttt tggagtaaaa
     5401 taatccgttg aaaaattcat aatcatatag tttgttcttc aatcttcacc tttgcagccg
     5461 cggttacaca atcgaacccg agacggggca acaaactagg gatgagttat tatttttctg
     5521 ctgcattatt ttacacaaat tacagtatat gatagatcat ccacgaatgt caagataatt
     5581 agtacataaa aacgctttac aaacctaggc tgtctttata aaatgagagg gtatgtgcaa
     5641 atggaaaaaa gcgaaggaag tttaaggaga aaatcaatga atccggtatc accattattt
     5701 gagaaaatcg atgcttcaat tgaaaagaag gcgaatcttt ttgaaaacag cttttcacgt
     5761 tacgctgttc gagcggttct cgcctgtttg tttttgactt taggaacggc tgtggcattt
     5821 gcgatcgcga tgaaggggga agatatcgcg catggtctag gaaagatttt atacgcattt
     5881 atgttcagct ggggtctggt catgattcta tacatgaacg ccgaattagg aacatcgaat
     5941 atgctttata tgaccgtggg tgtttttcgg cagagacttg ctttaagtaa ggcagccaag
     6001 attctatttg cctgtatttt tttcaatatg attggcggta tattatgtgg atttctaatt
     6061 tctatgacta cgccatttca agatttaacg ttggacaact ttatgttaga atcaatagca
     6121 gtaaaactag caaaaccttc agcaacgatt ttggtggaag ggatgtttgc gaatatcgtt
     6181 gtcaataccg ctgtactgat cagcatgcgg atgaaagagg atgtcggaaa agtgatcaca
     6241 gtcgtattca tcattttcat ttttgctttt ttaggatatg aacacgtgat agcgaatttt
     6301 cctgccttta cgcttactta ctttgcttct cacggacaga tggatggaat gacagtcagc
     6361 aatgtgttac ataatttgtt ttttgctttg gcaggaaatt atattggtgg cggtttagta
     6421 atgggactgg gatatgcttg gctgaatcag tcaaaatcat cctatgtgga ctaaaaagtc
     6481 gttcattaaa ggggaagtgc catggaagtt ttagtttttc caaaaaatga ggaaaaagcg
     6541 ttacaaatgg aaaagtacat gaaaaatctt ttttcctttg ctggagtacc taagccggaa
     6601 cgcgctcttt tacaacaacc gttactggat gagagcaaaa atttatctat ttctgaagtt
     6661 ttagcttttg ttcattatta ttatgaacaa atcgaaagag agtatcagta tgtagcgatc
     6721 gatttagtcc agaaaaacat caaacggctt acatttgaag acttagtcgt attgttgcca
     6781 ctagttcaga aaaaagaatg gtgggacagc atcgactctt ggagaaaagt atacagcaca
     6841 tggtgtaaag cctacccaga gcagcgtgaa gaagtctttc aattgtttga aaaaaaggaa
     6901 gatttttggc tacgtcgaat agcaatcacg ttgcaattag gttataaaca gcaaacagac
     6961 acaggactat tgatccgagc aatagacgaa gatcgtcaaa caaaggaatt ctttatccaa
     7021 aaagcaattg gttgggcatt acgcgattat agtaagaccg atgccgattg ggtaaaaaga
     7081 cagctgacaa cagatttgag taagttagct gttcgagagg caagtaagta tttataagaa
     7141 agcgagacga caagagatga gacgcaaatt atttgctttt gatattgatg gaacgttgtt
     7201 agattcaaac aaacaagctt tagatagcac aagagaagca ttggaaaaat tacgcaaagc
     7261 cggtcatctg gtcacgattg ctacagggcg gagccgattt catgcgcaag agatcattcg
     7321 cgatcttgat tttacgaact acattttatg taatggagcg gccggttttt tagaccatga
     7381 acaagtatat aagaatttac tagatcgcga ccagttggat gcgttcgttc aagaagcgca
     7441 tcatgagcag atcgatacag cttttgtcag cttagacaat atcaagcgtt cttcttccaa
     7501 tgatgtaacg gtgatggaag aagcgatgca ttctttcggt gctgtcttgc ctgaattaga
     7561 tcagtttttt gcagaggaac aagaaatata ccaagcgtta gcatttttcg atcaaacata
     7621 tgatgggaag ttctcttcat atgataaaat tcgatttgtc cgctggcatg agaacagtgt
     7681 agatgtcgta cctaaaaatg gttctaaagc agcgacaatt ctgcacttag cagaacaagt
     7741 aggcatccct catgaagaca tcatcagctt tggcgatgga caaaacgacc gtgaaatgtt
     7801 gcgcatgtca ggaattggga tagctatggg gaatgctgtg ccagaagtac aagcagaagc
     7861 taagatggtc actgatacaa acgatcagga tggaatttgg aaggcattga aagagttaag
     7921 cgtgatttaa aagaggttgt gaaaaagctg ttctgcatca agtaataagc ccagccaaac
     7981 aaaaatagct cctcctattt ttcgtttttc tgggcttatt accgcagatg caagctttcg
     8041 aacaccgttt aaggaagagg tcgtgacaga agtggtcagt ttcaagaaat aagaagggat
     8101 ttacgaaaat tgcttctcaa atttttgtaa aattacggct tatttctgaa gaagtagatg
     8161 aaacaacgtt tattcgggaa atgggactgt gacaagatgt ttgtcatagt cccatttttt
     8221 tattttattt ttcaaaacta agaaatacca ggaaagagtt ccttttgttt gaccttttct
     8281 ttcttattga aaacaactat aatatagagt gatgttaaaa gagaagagga atttagtgtg
     8341 cgggtattgt ttattggtga tgtagtaggt tcaatgggac gagagatgat tactgaatat
     8401 cttcctagat tgaagaagaa gtaccgtccg caagtaacga ttgtaaatgg tgagaatgcc
     8461 gcttctggac gtggaatcac agaaaaaatc tataaaaaat ttttgcaaga cggcgtagat
     8521 gtcgtgacga tggggaatca tacgtgggac aaccgcggaa tctttgagtt tgtaaatgaa
     8581 gctaaaaaga tggttcgtcc ggcaaatttt ccggaaggga cgcctggaca agggatggtc
     8641 tttgtcaaag tgaaccaagt cgaactggca gtgatcaaca tgcaggctcg ttcttttatg
     8701 gtggacttgg atgatccttt tcgtaaaatg aatgagctgg tagaagaagc cagaaaacgt
     8761 acgccgatca tttttgtaga ttttcatgga gaaacaacaa gtgaaaaaca agcaatgggc
     8821 tggtttttag atgggaaagt ttcagcagtg gtcggtacac atacacacgt tcagacaaat
     8881 gatgcgcgaa ttttgccaag aggaacagcg tatctgacag acgttgggat gacaggtccc
     8941 tatgatggta ttttgggtat gcgcagagaa cctgtaatcg aaaagtttct gactgcatta
     9001 ccaaaacgtt ttgaagttgt ggaacaaggc cgatcgattt taagcggctg tatcttggaa
     9061 ttggatgata cgacaggtca tgcaaaagaa atccagctga ttcagatcaa cgatgatcga
     9121 ccgttcatgg agtagaaaga gaggcagttt tttgcagcag gagccggaaa ttcaaaaaga
     9181 agtaaggaaa ctgacaaaac ttttaagaga gaatgaaacg atcattcgct acaaagaact
     9241 tgaagaaaag atccagcaaa accagtattt agctgagttg agagaaaaaa tcaaacaagc
     9301 gcaaaaagat gctgttcatt ttgcccacta tgacaaacca gcagcagaaa aagaagcaat
     9361 caaacaagcg gatcaattca tgcaagaatt tgatcagcat ccgcttgttg tagcttatag
     9421 aaaacagctg ttggaagcag atgatctgct tcatcattta acaactatga tacaagaaga
     9481 aatcaatgga caaatagagg aggaaaaaca tgcctcaaaa aactaaaaat actcctatga
     9541 tggaacaata tctaagcatc aaggcccaat accaagatgc ttttttgttc tatcgtttag
     9601 gagactttta tgaactattt tacgaagatg cgatcaaagc gtcacagctt ttggaattaa
     9661 cgttgacgag cagaaatcga aatgcagaag aacctattcc tatgtgcggt gtacctcacc
     9721 atgcagcaca aggatatatc gatgccttga ttgaaaaagg ctataaagta gcgatttgcg
     9781 agcaggtaga agatccaaag acgactaaag ggatggtcaa gcgcgaagtg atccagctag
     9841 tcactcctgg tacagtcatg gacagcaaag gattgtcggc aaaagacaac aattttttaa
     9901 cagctgtagt tagtcaagga aatgaatttg gctttgctta tgccgattta agtacgggag
     9961 aattgaagac ggctcggttg catgatgaag aagctgtttt aaatgaagcc tctgcattgc
    10021 agacaaaaga actcgtatta ggcagtgaga tcactgatac ccttcgtgaa cagctaagcg
    10081 ggcgtttagg gctagtcttt tctcaacaaa atgagatgga agaaaacgcg gaatttagct
    10141 ttttgacaag cgaactagtc gatccgctgg aaaaagaagc aaccggcaaa ctattgactt
    10201 atttatcggt gacacaaaag cggagtttag ctcatatcca aaaagcagta gaataccaac
    10261 cagatcattt tttgaaaatg gattattatt caaaattcaa cctagaatta agccaatcta
    10321 ttcggactgg gaaaaagcac ggcactcttc tatggttgtt agatgaaaca aaaacagcaa
    10381 tgggtggccg tttactaaaa caatggctag accgtccgct tatccaggca aaacaaatta
    10441 aagcaagaca agaaatggtc gcttctttgc tagacagcta ttttgaacgt atcgatttgc
    10501 agtcagcatt aacgaaagtc tatgatctgg aacgcctagc aggtcgagta gctttcggta
    10561 atgtcaatgg gcgagacttg atccagctga aaacttcttt agaacaagtg ccaatgatca
    10621 gagggttgat tgaaggaatc gatcaaggtc aatggcaaag tctgctttca gaaatgcagc
    10681 cgatggacca tctcgtccaa ttgatcgacc aagctatcca agatgatccg ccgttacaaa
    10741 taacggaagg aaacatcatc aaagatggat tcaatgagca gctcgatatg taccgttctg
    10801 ctatgagaaa cggaaagaaa tggttggcag aacttgaagc aaaagagcgt caagaaacag
    10861 gaatcaagaa cttaaaaatc ggatttaacc gcgttttcgg ttattatata gaaattacaa
    10921 aagcaaatct tggaaatgca gaactggaga agtatgaacg aaaacaaact ttagcaaatg
    10981 cagagcgttt catcacacca gaactaaaag aattggagac acaaatcctc gaagcagaag
    11041 aaaaatctgt tgatttagaa tatcagttgt ttttagctgt gagagaagaa gtcaaaaagg
    11101 ctatccagcc gctacaagta cttgcaaaag caatcagtgc agtagatgtg ctacaaagtt
    11161 ttgcaacgat cagcgaacgc tatcagtatg ttcggccaga acttgtcagt gacaagcatc
    11221 agcttctgat caaagacgga cgacatcctg tagtagaaaa agtattagga catcaggaat
    11281 atatccctaa cagcgtagaa atggcagaag acgaaatgat cttgctgatt acaggaccga
    11341 atatgtctgg taaaagtacg tatatgcgac agttggcatt aactgttctg atggcgcaaa
    11401 tgggctgttt cgttccggca aaacaagcag ttttacctat ttttgatcgg atttttaccc
    11461 gaattggtgc aagtgacgac ttaattgctg gacaaagtac ttttatggta gagatgatgg
    11521 aagccaatca agccctgcgt tatgctacgc caaacagttt gattttgttt gacgaactag
    11581 ggcgaggaac agcaacctat gacggaatgg cgttggccca agcaatcatc gaatatatcc
    11641 atcgtcatgt acaagcaaaa acactctttt ctactcatta tcatgaactg acggtacttg
    11701 agaaagaact tccgcagctt aaaaatgtcc atgttggagc cgttgaaaaa gacggagaag
    11761 ttgtcttctt gcataaaatg atggatggac ctgcggataa aagctacggc atacatgtgg
    11821 caaagatcgc aggattgcct tccaatctac tagaacgagc agcagatatc ctgcatacgc
    11881 tagaaaatgg cgataacgga caccatactg ttccagctga agtagaagag aaaactaaaa
    11941 cagccaaaca ggagcaaata aaaccacctg cagaaaaagt aaatcaagac catatacaag
    12001 accgtgtaaa agaagaaaac caacagcttt ctttattcag tgaattatcc gaaaatgaaa
    12061 cagaagtcat tgaatcactg aagcaaataa atttactaga aatgacgcca atggatgcac
    12121 taaacacatt atatgaatta caaaaacaac tgtaggaaga aggcggatat cgatggccaa
    12181 aatccaagaa ttatctgaac gtttagctaa tcagatcgct gcgggagaag tagtggagcg
    12241 accagcttcc gttgtgaaag aattagtgga aaatgcaatt gacgccggca gtactcagat
    12301 tgatatctta gttgaagaag cgggactgcg gacgatccaa gtgattgata acggagaagg
    12361 cattttggca gacgacgtag agaatgcctt caagcggcat gcgacaagta agatccataa
    12421 tcgcgacgat ctttttcgca tacgaacgct gggctttcga ggggaagctt tgccaagtat
    12481 tgcctctgtt tctgaaatga ctctggaaac ggcgaatcag gaagaaaaac aaggcaccta
    12541 tctgtcgctg aaaggcggag aagttattga gcatcgacca gcaccactgc gtcaaggaac
    12601 gaagattacc gtcagcaagc tgtttttcaa tacgccagcc cgcttgaaat acgtgaaaac
    12661 actgcacaca gaattagcga acatcgggga catcgtcaat cgactggcat tgagtcatcc
    12721 atcgattgct tttcgtttag tacatgatgg aaacaaaatg ctggcaactg ctggaaatgg
    12781 cgatctgaaa caaacaattg cgggaatcta cggattatct accgcaaaaa aaatgctgaa
    12841 aatcgaagga aaagatctag attttgagtt atttggctat gtttcattgc ctgaggtgac
    12901 tcgtgccagt aggaattact tatcgacgat catcaatgga cgttttatca agaacttttc
    12961 cttgaataaa gcaatcgttg caggttatgg gtcaaaatta atggtcggtc gtttccctat
    13021 tgctgtattg gaaatcaaaa tggacccgct tttagtagat gtaaatgttc atccgacgaa
    13081 gcaggaagtc cggctgtcga aggaaaaaga actaacagag ctaattagta acgccattca
    13141 aaaagcattg tctcaagaaa atctgattcc aagtgcagcg gataatctac ggtttaagaa
    13201 aaaagtacct gatcagccta aaacagaaca aatcgaaatc gatttgtcct acgcttttga
    13261 agaagaaaaa cctaaaaagc caagtagttt gaattttgat cgagagacag gtaccttttt
    13321 cgtagaagaa ctaaagaaca catttgttga cagaattgtt gataagtctg tggaaaatgt
    13381 tgaaaactat tctggttttt ctgaagaaaa caccacttat ccagaaaaaa cagcgaaaac
    13441 ggtggataag tttggtgaaa atgttgataa ctttgtagaa aaaacagtgg aaaaatctga
    13501 aaagaaagaa tttcccattg tggataacat ggagaaaaca aatgaagaac aagcagataa
    13561 atccgtagaa gaggatgaaa agatttacga ggaagaattg acactccatc ctgaatttga
    13621 tttcactcaa ggaaacgaca aaacattaga acaagccatc aagaaattag aaacggaaaa
    13681 accagtacaa cgttttcctc agttggaata ttttggacag atgcacggaa cgtatctttt
    13741 tgcccaaagt agcgatgggt tgtacatcat tgatcagcat gctgcccaag agcgcattaa
    13801 gtatgaatat ttccgggaaa aaattgggga agtatctgat gatctgcaag aattgcttgt
    13861 tccatttgtg ttagactatc caaacagtga tgctttgaaa ttaaaagagc aaaaacactt
    13921 gctagaagat gcagggattt atttagaaga atttggtcaa aacagcttta ttgtacgggc
    13981 ccatcctact tggtatccca atggagaaga agaatcgatt attcgggaga tgatcgatat
    14041 gctgctgaca actggaagtg tgagtgtaaa aaaattccgt gaagcaacag caatcatgat
    14101 gagttgcaaa cgatcgatta aagccaacca ttatttgaat gaagcacaag cacgtgtgct
    14161 gctgaaggat ctggcacgat gcgaaaatcc tttcaattgt ccccatggcc gtccagtatt
    14221 gattcacttt accaattcgg atatggaaag gatgttcaaa cgaatccaag acccgcatta
    14281 agtcaaagag aagagggaaa acatgaagat tgtattagca tcccaatcac caagaagaaa
    14341 agagttattg gctcatatcg tttcagaatt cgagattgaa cctgctgata tcgatgaaac
    14401 gccatttcca gacgaacagc ccgtcgatta tgttcgtaga atggctgaag aaaaagcacg
    14461 aactgtctgg gaacaagatg aaaaagaaga ccatttagtg attgccagtg acacgactgt
    14521 tgttctcaat caagagatca tgggtaaacc agaagatctg gctgaggctg aattcatgct
    14581 gaaaaaacta agcggggaaa cacatactgt atatacagct gttgttttaa aaacaaagga
    14641 acgagaagaa cggatactag cagaagcaca tgtcactttt tatcctttga cagacgaaga
    14701 gatcaaacgc tacttggaga cgggggatta tgcggataag gcaggggcat acggcatcca
    14761 aaatgctgcg agtgtattcg tcaaagaaat ttctggagat tattacagta tcgtaggatt
    14821 tccgattggt gctgtcaatc aggcactgaa aacattcatt taaacaaaaa agcggactat
    14881 ctgattttcg attgaaaatc catgatagcc cgtttttttt attgaaaaga ttacttatct
    14941 actctgacta agattttact tcagctttac cgactgcttc gcccgggcga acaagtcctg
    15001 attggtcaag agaaagtcct gcaacttttt cattattggt caaagcaaca ataatgtcag
    15061 ttttcttgcc agcttgttct atttctggca aatccatttc agcaatcagg gtctctggtg
    15121 tgacaaggta tccttctttc acgaatacat tgaaaggaac accctttagt tcaacagtat
    15181 ctaatcccat gtgaacaagt acttccagcc cctcattaga aagaatacca atggcatgtt
    15241 ttgtttcaaa gacactcgtc actttaccgt ttactggtgc atagactttg ccgtttgacg
    15301 gttcgacagc ataaccgtca cccatcattt tttgtgagaa aactgggtca tttacttcag
    15361 ttatttcctt gatggtccct gcagcgactg cgaataatgt ttcttttgtt gcaactgccg
    15421 taccagttgc tgaaacaggt tcgaaagact gtggcgttgc tgttgcgcca tttgctgttt
    15481 gaagttgtac atcagcagca ttttcaggaa cagcaggatc aatcttattc aaaattccct
    15541 gacgtctaaa gatgatcgtc aagccaaatg gaatgatgac agcaatgatc attgcgatga
    15601 tgaaaggtac ccatgtttct gcttggatag ctagtattcc tggcaaaccg cctactccga
    15661 tcgcgttagc tcgaacaccc atcaagttag caaacatgcc agctaatcca gagccgacca
    15721 ttgcagcgac gaaaggatag acgtatttca aattgattcc gaacatagca ggttcagtta
    15781 cgcctaagta acaagaaatc attgctggaa tcgagatttg ttcttctttt ttattcccac
    15841 gatgtaagaa gatgatggct aagactgcag agccttgggc gatatttgat aaagcaatca
    15901 tcggccataa attcgttgat ccgaaatctg caatcagctg catatcgatg gcattggtca
    15961 tatggtggag accagtaatc actaaaggag catacaggaa tccgaatacg gcactaaaca
    16021 accagctgat agaagacgtc aatccagtat ttacgatatt tgagatccag cttccgactg
    16081 tccagccgat aggacctaaa ataacgtgtg cagctaaaac agttggtaac aatgaaaaca
    16141 atggaacaaa aatcatcgag atcgactgag ggatatattt gcggaagaag atttctagat
    16201 acgctaacat aaaccctgcc agcatggctg gaataacttg cgcttgataa ccaatcatct
    16261 ggacttgagc aaaaccaaaa tcccaaaatg gaatatctgc tgcggctgtg gaagctacac
    16321 tataagcatt taataattgc ggagaaacta acgtaattcc taaaacgatt cctaatattt
    16381 gtgtagtacc catctttttt gcaatactcc aagtgatacc tactggaaga aaatggaaaa
    16441 ttgcttcacc cggtaaccac aaaaaggcat tgacaccatt ccaaaacttc gagtgttcaa
    16501 cgattgttcc accaagactt tcaaactgga taccttcaag tacatttctg aatccaagga
    16561 tcaatccacc gacaatgatt gcaggaatca gtggtgtaaa gatttctgct aaaacggcaa
    16621 tagcacgttg aacgggatga aggttttgtt ttgcagctgc ttttccttgt tctttggaaa
    16681 ccccttcaac tccagatact gcaacgaagt cgttataaaa agtagaaaca tcgtttccta
    16741 tgatgacttg gaactgtcct gcattcgtga acatcccttt tacgctaggt atatcttcga
    16801 tcgctttttc gtccgctttt cctggatcat tcaggacaaa acgcatacga gtcgcacaat
    16861 gactaactgc agcaatattt tcttttccgc cgatatcatg taacagcttc tcggcatctg
    16921 cttgatattt tgccatatca ataaacccct ttctcttaac ttgtctatgc aagttgtttc
    16981 ttcatttact atactttttt tcggagagaa ttgcaagcgt tttattaaaa agtacgagag
    17041 aaaatgatat cgttttaaaa agcttaaaat aagcttttct taaaaatagg aacattgaaa
    17101 tacaaaaaga gctgtagtat agtgaagaaa tgatggaaga acgagtgagg gaaaaaacat
    17161 gaataagttc aatgaaattt ttttagattt ggaacagaaa attttagcga aagaatatcc
    17221 tccgcattcg cttttaccca gtgagaacca attgatcaaa atttatggtg tttcacgaga
    17281 aacaattaga aaagcgctga atcttttgac tacggaaggt tatattcaaa aaaaacaagg
    17341 aaaaggttcg atcgtactgg atcggaatcg cttcgacttt cctatttctg gattgactag
    17401 ctataaagag ctacaggaga cccaacgtat tccaagtgaa acgatcatcc atacgttaga
    17461 agaaacagaa gtgacaaaag cattgaacga gatcactggc tgggagatcg gtgcgcctgt
    17521 ttggcatttg atacgcgaaa gaaaaatcaa tggcgaagta gttatattag acaccgatta
    17581 tctattaaag gaaatcgttc ctattttaac tccagcacaa gcacaaggat cgatctatga
    17641 atattttgaa aatgagcttt ctttgacgat tgcttacgcg caaaaggaga tcacagtaga
    17701 agaagtaacg gaaatcatga aaacagcgat ggatcttcac gatgacactt atgcagtagt
    17761 agtcagaagc ctcgttcatc tggaagatac ccgctgtttc gagtattctg agtcggttca
    17821 ccgattagat aaattccgtt tcgttgagtt tgcccgacgc cgaaaagtgt aaatagttca
    17881 aaagaaagca aattgttggc atctgtccac tttggcgcta tgataggatc acaaaggggg
    17941 catttttaat ggataaacca ataagaccag aaaaagaaga actgaaagaa aagttatcac
    18001 ccatggctta tgcagtcaca caagaaaatg caacagaacg accatttagc gggaaatatg
    18061 atgattttta cgaaaagggg atctatgtag atatcgtcag cggtgaaccg ttattcagct
    18121 ctgcagaaaa atatgatgca ggttgtggct ggccatcatt cagcaaacca ataacgaaaa
    18181 gacaagtgcg cgaaaaagcc gatttttctc atggcatgca tcgaatagaa gttcgaagca
    18241 aagaagcaga ttctcatctc ggacatgtgt ttaccgatgg accagtagat caaggtggtc
    18301 tacgttactg catcaattct gcctcactga aattcattcc atatgaaaag atggatgaat
    18361 taggttatgg ggaattcaaa tcacttgtag aataatggaa atacacaaag agcggtacaa
    18421 tacggggatt tactcgatcg tccgctcttt ttttattcaa gcgattgcta tttgaaagaa
    18481 acgctcccta gactcctttt ttcacaaggt gaaatgtgct acaataaagg gaacgtatgt
    18541 gtgcaaaaga aagaggacta gaatatgtat gaatatctga caggtgtggt tacctttatc
    18601 aacccctatt acatagtagt agaaaccaat ggtatcggct accagatcgc actaggaaat
    18661 ccttaccgct acagtagcaa actaaatcaa aaaataaaaa tttatgtcca tcaggtaata
    18721 cgtgaagatg cgcatcttct gtatggattt gattcgctag aagaaaagca actgtttttg
    18781 cgattagtaa gtgtttcagg aatcggtccg aaaagtgccc ttgcaatcat ggcaagcgaa
    18841 gaccactccg gtttgattca agcagtagaa tccagtgatg tcacttattt gacgaaattt
    18901 ccaggagtag ggaaaaagac agcacaacaa atgatcttgg atctaaaagg aaaattcggt
    18961 gagctgagta tcgatacacc ttttagtcta tttgatgaag caaacacaaa agacgctaca
    19021 gctctatctg aagcaatgga agctctctcg gctcttggtt acagtgatcg agaaatcaaa
    19081 cgtgtagaaa aacaattaaa agaagtagat aatcagacaa cggatgagta tctacgccaa
    19141 gcactcaagc taatgatgaa aaaataagga ggagaccaaa tgacagatga atacttgtta
    19201 tcaccagaat ctggcgaaaa tgaactgagt ttggaaaaat ctcttcgacc acagctgctt
    19261 tctcaatata tcggacagag aaaagtcaaa caagaacttc agatttacat cgaagctgcc
    19321 agaaatcggg aagaaccttt agatcatgtt ctgttgtacg ggcctcctgg attaggaaaa
    19381 acgacgatgg ccatggtcat tgctaatgag atgaccgtaa atattcgtac gacgagtggc
    19441 cctgctatcg aacgtccggg tgacttagta gcaatcttga atgaactgga agcaggggat
    19501 gtcctgttta tcgatgagat ccatcgatta cctagagtgg tagaagagat gctttattca
    19561 gctatggaag atttctatgt agatatcatg gtaggacaag ggccaaccgc acatccggtt
    19621 catttccctc ttcctccctt tactttgatt ggcgcgacga cacgggcagg tatgctttct
    19681 gcacctttac gagatcgatt tggcatcatt tctcatatgg aatattatga cgaacaggat
    19741 ctgcaggaga tcgttttacg ttcttcggat atttttcaaa cagagatcat tgaagaaggg
    19801 gcattggaaa ttgctcgccg atcacgagga acgcctagaa tcgctaatcg cttgctcaaa
    19861 cgggttcgtg attttgcgca agtccaagga gacgggaaaa tcgatcgtcc tatagcggat
    19921 caggcactta ctttgctaca ggtcgatcag gctggattgg attatgttga ccagaaattg
    19981 ttgcgaacaa tgatcgagtt atatggcggc ggaccagtag ggttaagtac gctttcagta
    20041 aacataggag aagagcgtga gacggtagaa gatatgtatg aaccgtatct gatccaaaaa
    20101 ggatttttaa agcgtacacc tagagggcga attgctactg cttatgccta tgaacatttt
    20161 ggttacgact atcatatatg atttttgctt ttagattgga ggaaaatgaa tgagtgatac
    20221 aacacgtacg aaacaattga tcaaaaacgt attgattgat ttgtgccaaa cgcaatcttt
    20281 tcgaaaaatc agcgtccaaa aaatcgcaaa cgaagcaaaa atcaatcgac aaacatttta
    20341 ttatcatttt acagataaat acgatttgct tcgttgggtc tattgtgagg atgcattgca
    20401 ttatttagat ccacaatgtc tttcacttga taattgggaa gagcaagcat tgaaaatgct
    20461 gaaagcaatt gctgagaaga aagaatttta tgcaaatact gtgctttctg acccagaaat
    20521 tttacaaaaa gagttttctc aactgatcca aaaatcattt atcaagattt ttgaacaaat
    20581 ggatgaagaa agacaacttt ctgcaagaga caaactattt tatgctcgtt ttttttctta
    20641 cggttgcagt ggtgtattag tcaattggat cacgaatgaa tatgaagaaa caccgatgga
    20701 aatcgctatg cagcttttcc gtttagccaa ggatacggaa ttatattctt atcgtcttta
    20761 tgcaaaagaa gagttggaag aacagtaata gaaaaatttt ttgttggaaa agcaataatc
    20821 gctaaaagga gatggggaaa tgacaaaaat tttatttgtt tgtttgggaa atatttgccg
    20881 ttcaccgatg gcagaaggct tgctgagaaa acgaatagca ttagaaggaa gggaagcaga
    20941 attttttgtg gattctgctg ctacaagcac gtatgaagtt ggaaagacac cgcaccctgg
    21001 aacgaaaaaa atattaaacc aagaaaaagt agatatgacg aatatggttg ctcggcaaat
    21061 cacaccccac gattttgaaa cttttgattg gatcattggc atggatcaag aaaatgtaga
    21121 agaactgaaa cgaagagcac cacgctcagc gcaggggaaa atccatttgt ttttaagtag
    21181 cattcctgga aaagaaaaag aaaatgttcc cgacccatac tataccaata actttgatga
    21241 gacatatcac ttgatcacag aaggcctgaa tcattggtgg aatatttgga ttgatgaaaa
    21301 taaaataaca aacaatttgt aaagttaacg ctttcttttt aaggcgatgt tagagcattt
    21361 ccttgttttt ttgtaaaatg ttaggtaaaa taaaattaat taagtaacac tttcttagct
    21421 aaaggaggag tgcattcgat gaacgaagag acagtatcgc caatgaagaa gcttacgaag
    21481 ttagttactc atacttcgta tcaacttttt gattcttttg taaatgacac tgaagaacat
    21541 gctaagaatg tacgattgca aggaaagttt attcttgagc tgcaaaaagc tgctactgaa
    21601 catagtctag ttatcttaca agtaagagca aatcgtttga gtagcaagta tgaaacgatc
    21661 tcaggctggg tggcgacaaa aacggtcgaa aaagaccgag tgatgatccg cctacaaaac
    21721 gaagagcagc agatccggat ggttcctgta gaacaaattg aaaaaataac aatcctttca
    21781 ccagcagggg aacaggaacg gttatcaaaa taatttatac cagatagaaa agcacgtatg
    21841 agcttgtacc atcaatcatt actaatcaaa aataaccgaa tgctttaagc tgatatcagt
    21901 aaaacggaac gttttaggat gagtatcata gcatgacagc attcggttga ttagttgaat
    21961 cgattggatg gtacaagttt ttttgataaa aaaaggtcct gctagccggg gagctagcag
    22021 gataaggagt taaaaatgaa aaagtgttgt tagggttgtt tgttggtatg cttatataat
    22081 agtcattagt tgtgaatatt ttgtgacgaa agttttcgaa agttgttaag tt
//
LOCUS       DAFEOM010000038        21501 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.039, whole genome shotgun sequence.
ACCESSION   DAFEOM010000038
VERSION     DAFEOM010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.039"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            2..400
                     /locus_tag="LP524_001670"
     CDS             2..400
                     /locus_tag="LP524_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBL2234727.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            384..1079
                     /locus_tag="LP524_001671"
     CDS             384..1079
                     /locus_tag="LP524_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBL2234728.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1663..2550)
                     /locus_tag="LP524_001672"
     CDS             complement(1663..2550)
                     /locus_tag="LP524_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBL2234729.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2735..3574
                     /locus_tag="LP524_001673"
     CDS             2735..3574
                     /locus_tag="LP524_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBL2234730.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3770..4744
                     /gene="sgrA"
                     /locus_tag="LP524_001674"
     CDS             3770..4744
                     /gene="sgrA"
                     /locus_tag="LP524_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HBL2234731.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5042..5398
                     /locus_tag="LP524_001675"
     CDS             5042..5398
                     /locus_tag="LP524_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234732.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5401..6558
                     /locus_tag="LP524_001676"
     CDS             5401..6558
                     /locus_tag="LP524_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2234733.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6584..7024)
                     /locus_tag="LP524_001677"
     CDS             complement(6584..7024)
                     /locus_tag="LP524_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBL2234734.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7184..7690
                     /locus_tag="LP524_001678"
     CDS             7184..7690
                     /locus_tag="LP524_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL2234735.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7858..9771
                     /locus_tag="LP524_001679"
     CDS             7858..9771
                     /locus_tag="LP524_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBL2234736.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVEKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9776..10777
                     /locus_tag="LP524_001680"
     CDS             9776..10777
                     /locus_tag="LP524_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL2234737.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10979..11185)
                     /locus_tag="LP524_001681"
     CDS             complement(10979..11185)
                     /locus_tag="LP524_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HBL2234738.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11831..12679)
                     /locus_tag="LP524_001682"
     CDS             complement(11831..12679)
                     /locus_tag="LP524_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL2234739.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12678..13745
                     /gene="gnd"
                     /locus_tag="LP524_001683"
     CDS             12678..13745
                     /gene="gnd"
                     /locus_tag="LP524_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HBL2234740.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            13747..14748
                     /locus_tag="LP524_001684"
     CDS             13747..14748
                     /locus_tag="LP524_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBL2234741.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14745..15029
                     /locus_tag="LP524_001685"
     CDS             14745..15029
                     /locus_tag="LP524_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBL2234742.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15062..15859
                     /locus_tag="LP524_001686"
     CDS             15062..15859
                     /locus_tag="LP524_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBL2234743.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15873..16706
                     /locus_tag="LP524_001687"
     CDS             15873..16706
                     /locus_tag="LP524_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL2234744.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16724..17581
                     /locus_tag="LP524_001688"
     CDS             16724..17581
                     /locus_tag="LP524_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL2234745.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17603..18112
                     /locus_tag="LP524_001689"
     CDS             17603..18112
                     /locus_tag="LP524_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2234746.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18149..18550
                     /locus_tag="LP524_001690"
     CDS             18149..18550
                     /locus_tag="LP524_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234747.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18662..19015
                     /locus_tag="LP524_001691"
     CDS             18662..19015
                     /locus_tag="LP524_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234748.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19049..19492
                     /locus_tag="LP524_001692"
     CDS             19049..19492
                     /locus_tag="LP524_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBL2234749.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19509..20594
                     /gene="uxuA"
                     /locus_tag="LP524_001693"
     CDS             19509..20594
                     /gene="uxuA"
                     /locus_tag="LP524_001693"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBL2234750.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20587..21300
                     /locus_tag="LP524_001694"
     CDS             20587..21300
                     /locus_tag="LP524_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL2234751.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         7399 a         3302 c         4490 g         6310 t
ORIGIN      
        1 gatgacgatc tttgaaacgg ttgtaacaga tgttggtata gaagcagaag tattcaggaa
       61 agaaaaaata atgattttat ttggagatga agcaccggaa acattaacag attattgtta
      121 taagattgaa ttgaatcagg taagaaaaga aataacttgt gatatgaagc tctgttttga
      181 taaacaaatg tatgtaatca cagcagtagg agatgttgtc caaactaatt tgaatgaatt
      241 aggacatatc acgataaaat ttgatggatc tcatgagccg gaacttccag gaacaatgta
      301 tgtagaagat aaggaaatgc cgcatttaag aaaggggaca atcattaaaa tcgatcaaga
      361 ggatcagaat gggggaaagc tatatggaaa tcatgcttga tacagcaaat acaaaaagta
      421 tttattcttg tcaacagttt ttgaatttaa caggcgtaac aacaaatcct acaattctga
      481 aaaaagaagg agcgtcaaac ttcttctcgc atatgaaaga aatcaaaaaa ataatcggac
      541 agattcctat ccatattcag acaataggaa gaacggaaga agaaatgata gaggatgctc
      601 gagttatcat agaagaactg ggaaaagata cgtttatcaa ggtgccggtg aatcaagcag
      661 gccttcaagc gatcaaaaag ttgaaaaaag aaggttattt gattaccggt acagctattt
      721 acacagaatt acaagggtat ttagctatta ataatggagc agattatatt gcaccttatt
      781 ataacaggat gcaaaatcaa ggcattgatg ctatgaaagt gattgattcg ttgcggactg
      841 agatcgaacg tacgcaaaga ccaacaaaaa tattagcagc tagtttcaaa aattgtgaac
      901 aagtcactac agccattcga tctggagctc atgcgataac gttaggtaaa gagctactag
      961 atcaagtatt agagatgccc tcgattgata aagcagtgag gggcttccaa gaagattggg
     1021 tgcaaacttt ccaagcagaa acgattggtg aactaaaaaa ggtcgctgat tacttctgaa
     1081 tcaatttctc cataaaataa acgatagcct tctcgaaaac tcgaggaggc tatctgacga
     1141 ttcaatattc attaatttaa agtttgggtg actattactt atttctcatt taaacgatgt
     1201 attccctaac ctctaataca tccttgtaac tctgaagtta cgccagtttt gaataaattt
     1261 cataaaaaca aatgaaaagt gtcgcataaa aatatctttc gtgtttctct agtataacag
     1321 aataagagga acaagtccag aagggatatt tttagtcttt tttaaaataa cttttcagtc
     1381 gagtttgagt ttatgatagt tgtttttgaa gcgatacggc aagtgaaaaa atagggaagc
     1441 atgaaagaat ctacttaatg gcgcattgat tcatgtgata gaaataaaaa acttttttaa
     1501 attatatcct catgattgta acaaatccag tgatttgttt tggtacaaat aaaaaaaggc
     1561 cccgatgaag ttgtggcaaa agtggtttgc ctcaaaaata agaaggaatt tccaaaaatc
     1621 acttctcaaa ttttgccaca tccccaattg ggtcaaattt gtttatttga atatattttt
     1681 tacataagga tctttaatag ttacattgtc ttttttcatc actttacgga ttgttttatc
     1741 catgaattcc gtatcaagag tttttgcttc ggtagcaatt ttttctagct cagatttgta
     1801 tttgtcttta ttagaacctt tatcctgttt ttttaccatt tttactagat aatagctttg
     1861 ctgataagtc gttggatctg ttacaggaat gatatcagaa acttccccgt tttttagttt
     1921 aaatgctgct ttttttactt cagtaggaat ctctggattg gttgaatcaa aattcatttt
     1981 tccatcagtt tcctttaatg ttttattttt tccataagct tgaacaagct tggagaaatt
     2041 ttccccttca tttgcttctt tttttgcttt atctgcttct tctttagtcg aaaatagaat
     2101 gatctgtgta ctaacttctg gatagaattc tttccaagca gtgtctagat ctttatctgt
     2161 taattttaca ttggctttca aaccgtattg gaaagccagc atctgtttaa agagtttctt
     2221 atatgtctct tctgtttggc caactgcttt taattgttcc ttgtagtttg gaccgagttt
     2281 tttgatttgt tcattatatt cttttgttac ttcctttgaa gaaatctctt ttccgtatac
     2341 atcatcaaat gctttgaaga caatcatttt ttttaatagt gtttgattat ttgatttaac
     2401 ttttgggtta tcataaaaat cctgaactgt aagtgttcca cctttaaacg ttgcaatcgt
     2461 ttcattggac ttgctacaac ctgagaatag taaaattgca catgctgaaa ggcacataat
     2521 tagtagttgt tttttcaaaa aatttcccat cctttcctta ttaaaaacag tctaacagaa
     2581 gaattaggga gacagtcttt cattttcgta aggttgctgt agatattctt agttcgtaaa
     2641 aatctattct ctctatgatg gagaacattt acatagtttt cacgagacaa gaaagaaaag
     2701 ggttgttaaa cttaatataa aggagggatg aaagatgatc aggattattc ggttgattgg
     2761 atctgtttta atagtcttag ttggttttgt tttaggaatt cttgtcaata atgcacttgc
     2821 ggatatgttt attggaaata gtgaatggat tggagcaatt gccggtacgg tcggacattt
     2881 agctgttttt ttactagctt tatttcttgc tagtaaggtc gaaggaaaaa gaatagaaga
     2941 ttatggtgtt tctgttcgag gaaaagactg ggggtattta gcgggaggtc tcgttgttgg
     3001 aattggagtg tttctgctta ttactagtcc attatatttg acgggggcgt atcggttaga
     3061 ttcaggaaac gcaaacattg tgccgctgat aacgagtttt attttattta ttgcagtggg
     3121 ggcatcagaa gagcttttat tccgtggctt ttttcagcat cagatgctta gatttggtcc
     3181 gttaatagca atgattggat ctgctgcatt atttgctctt ttgcacggat tgaatcctaa
     3241 catgacattt ctggcagttt tcaatatctt tttagcaggt tgctttttca gcgcacttat
     3301 ctacagtaca ggaagtttat ttacggctat tggtgctcat attacttgga attggacaca
     3361 aggagctatt ttgggtatac cagtcagcgg aacgcaagaa caaggttttt tctccactct
     3421 gatccaatcg caaaatacaa taattactgg gggaaacttt ggggctgaag catctatatc
     3481 aactacatta ttcttgttag tcttgattgt tggtttgtta ctctatgcat ataaaacaaa
     3541 gcggatgaag aaatacactg ctgtttatca ataaaagatg aagaaagcaa ggctgatatc
     3601 ggtcttgctt ttttaagtct gtcattgatt aaacgaaaaa gtaaacaaat tcaagcgaat
     3661 acacgacatc ttttgttaga ttttcacaac tgactgtcgt gttctcatca ttttcatttt
     3721 atataattcc tttatcaaat agaaggtaat tatatggaga gtgatagaaa tgaagaaaac
     3781 agcgaccgta ttatttgtta ttcttgggct gagtataatt gcagcaatga ttggaatcag
     3841 tacttctgca aatgaacggg caaatgagaa tctttcattt actgtgaaaa ctgatcggat
     3901 tgtttatgac atgacacaac aagtcattac tattccagtc aaaccaaaca aaagcgtgaa
     3961 tgctagtgat gtacatgctg ttctaacata tggttgggat ggtaatggaa gttcagaaaa
     4021 agttattggg gaagtttatt taaaagacgt acaatggacc gctggtattg agtatacaat
     4081 aatgatttct gcagaactat cgattgatga gatcaagagt aaagataaag ttgatttgat
     4141 tgttttttat gatggacaga tgacgatcac ggagaatttg aaaccaagca gttggacagt
     4201 tgttggaccg ccaagcagta cggatagcac aagtagtgaa agcagtacag aaaacacgag
     4261 cggtgaaagc agtacagaga gtacgagcag tgaaagtagc acagaaaaca cgagcagcga
     4321 aagcagtacg gagagtacga gtagtgaaag tagcacagga agcacgagca gcgaaagcag
     4381 tacggagagt acgagtagtg aaagtagcac aggaagcatg agtagtgaga gtagcacagg
     4441 aagcacgagc agcgaaagca gcacaggaaa agtaaataat gaacttgatt tggtacctat
     4501 cataccaact aaggaacaaa agagaaatgc agggagcaac ctaaaaagtg tgctaaaaca
     4561 gcctgatttt gtttctcgtg gaattgatca tcaagaaaaa gcgaaagaaa cagataatag
     4621 aaaaagcgat ttaccagaaa ctggtagtag aaccttgaac aagctaatta catggatggg
     4681 tgtgttactc attttgattg taggagcaag ctattttcgt tctctcttcc atcgagtgaa
     4741 ataaagaata aaagcaacag atctaaaatt gaataaaacg atagattttt gagtgaatat
     4801 ttcattcgag agtgctgtat tgcctggcga agttgtagct ttgccagatc tatttttgtt
     4861 tactaacata tttgagaaac aggaaataac aggaacacaa atggaaaata agagcaagaa
     4921 taattgacaa tgcacactgc gcatggtagt atctctgaaa aatcagtaac gaatctattt
     4981 gtacgttatt catgtagctg tcggaataag tctaaataat agataaaaag gagtatgtac
     5041 tttgtttgaa gaatttattg atatcaatga aaggcaagta tatcaatttt tgaactattg
     5101 ttatgaaaga gacgagaaat tatatgtggt gaaggatatc gctttagatc taaactacac
     5161 cctggctaag atgaattcgg tgattcaaca agcagaatcg ttttgcgaaa ggtatccaga
     5221 atataaatta tcttttcttt cagaaaacaa gatgataaaa gtcgagtttt cttctcagtt
     5281 tttactgagt aaagtttatt ctatactttt agagggaacg attggttata ttttactgga
     5341 ttcactttac aaaggtactt atcaatcctt agaaaatctt agtcaaaaaa ttatataagt
     5401 ttgcgtacga ttcaaagaaa attaagagaa cttaatacta ttttgaaaaa ctatcattta
     5461 tcttgcagct tgaaaaaagg taatccgata gaaggagaag aataccgtat ccgatacttt
     5521 tttcacttga tgtattggca gtcattcgac gaaaatcatt ctgatatatt atcaagtata
     5581 aaaaataaac gtcaactatt agaaaaattt gcgaaacatg cgccatatac gcgtagtata
     5641 gatagaaaga aatggatcag cctactaaga atttcgttgc aacgaatgaa acaaggccac
     5701 tcgattacgt atgtcccaga agagatgaac aaatttgtgc atccattgat aacaaaagaa
     5761 gagtttacgc attacatact agagcctttt tttgaagcaa acttctggtc gatgaatcac
     5821 atcactccaa cagagcaagc ttacctttat tttatgtttg gtgtaatgaa cacctatttg
     5881 gaagaagatt tgttggatac tccagataaa gtacaagaaa gttggggctg gattgaatcg
     5941 tctattgtag aaaattttga aaaaaacttc aagatttgtt ttacaacttc agaaaaaaat
     6001 tatctatatg tcaatttggc aatgatccat ttgtattcaa gggtttttgg gacaaaaaag
     6061 aaggtggatg cttttggaaa atcagcagaa gacagagatt ttcagcatat atttccagtg
     6121 atgtatccaa tcatgcaaca gttttttagt ggggtagctc gcagaattcc agagcttaat
     6181 acttattatg aaaagaacag acggttgatt tttcaatact gtatgttagt cagggatatt
     6241 tttataaaac acgaacaacc tgtcatattc tatattcaat caaagtatgg taaaacacag
     6301 gaattatggg caaagaaaag gatattatct atgactgaac gcccaataaa atatagtgca
     6361 tccgctgatc agttacctga tattgtcatt agcgattatc ctatcgacaa aaaggcatat
     6421 gatcaaacga atacacagat attttactgg aatggacaac ctacaaaaaa tgattgggcg
     6481 cgtctgaaag ctgaaatcat aaaaatcagg aatgagaaaa atagatccat attggaaaaa
     6541 ttggatttcg ttaattaaga aaagcagatt gtttatgcca aaattaagac attgcaaaaa
     6601 gattattttc tttaaaatcg acaagatgac cagaaagcaa gagttcgttg agctcttgct
     6661 ttcttttagt tttagaaagg aacatcctga ttttatcttc gctaaatcca gtcaaaagtt
     6721 ttgcagattg gtcgtagata atcggttttt tcaataaaga aggggatgta cgaatctcaa
     6781 tatatagttc actcaaactg agttcatcta ttctgtgaac aagattttta taactaatag
     6841 aatgagtaga gagcatttcg gtcacgccgt tgtttgtcaa ttttagtaag tggataaatt
     6901 catttttggt tagtggttga cgaattaagt tctgttcgat aaacggtata tcatggacga
     6961 taagccaacg ttttgcgcgt cttgaagacg agcagccaga acctgtatac aatttaatca
     7021 tcatacattc ctccaatagc taagtattta tgaaataata acatgaagaa atagtcgaaa
     7081 agtgccattt agaggtgtaa aataagaaaa tgttgtcacg tataatggaa ctagctagat
     7141 aagaaatact attaaatcaa gaaaaaagaa agaaggcaca agtatgtcaa acacagccga
     7201 cgcaatagta gtgatcgatt tacaaaaagg ggtatgtttt gattcttcgc cgatacacga
     7261 attagaaaca gtgacagcat tagtgaataa aagaatcaaa acatacgaaa cgattgggaa
     7321 accagttatt ttcgttcaac acgaagatgc tcaattagtg ccacaaacaa aaccatggga
     7381 gctattgtcg gaattatcta ttcctgagca cagttatttt gtgagaaaaa cccatgcgaa
     7441 ttcattttat aagactaatt tgagtgtttt attaactgaa ttggactgtc attcgatcga
     7501 attatgtggg gcacaagcag agtattgtat agatacgact gtgaagtttg ctcatggatt
     7561 aggataccag cttatgatgt gtagaggagc aacgacaact tatgataatc cttttatgaa
     7621 agcaaaggaa acaatcgctt tttacgagaa aatttgggat aaacgatttc tgacattttt
     7681 ggagcaataa gttgtttctt cgtttaaaaa aagcagaatg gatactggtt tatccattct
     7741 gctttattac gtaaaatgag aaaataatcc tgttctattt ttatgggata aaccatttat
     7801 tttttgtata aaaaatgttt gcgtttacat ataattctcc tagaaacata aaaaggagtg
     7861 aatagcttga gaacaaaaca agtattaaag ggagcgttgg tcatcttatt tgctgtaagt
     7921 acaagcgtcc cgcaatggac agtatatgga gagagcattt cttccgaatc ggaatctcgg
     7981 gaggtagaaa ccacagaaac tactgatgct ggaaatgaaa cgaaatcgag tacttcacaa
     8041 acagaaacaa atactgaaac aacggttgag acatctgtta ctagcacaga tcaagaaacg
     8101 accgctacca cagataacca acaaacagaa gaagcaacag ctacaaaaat gtcaaacgac
     8161 gaatcctctt ctgctaaagt aagtgacaaa gagaatggga cagaaaatga tgatatcggt
     8221 aaaggtgaaa atttcaatgg taaagaatat ttgaattact tcggtaaaca gatgacagaa
     8281 atacttaatg gaccaacaga agacatcaag tcaaaattaa tagagttatt agtcactgag
     8341 cttgctgtta atgattatgg tgtagaaaaa gagattatca aacatctgat tgctacttat
     8401 ctattgtacg atatcgcttt tgcaaaaaac agtgtgttga tgtcgatgct tggaggtaca
     8461 gatgatggga caacgcctac agaaaacaca ggcttctatc caaatacaaa acaacttcat
     8521 caaactgtac taaacaaaca agatggcaaa atgatggatg tatatgcata ttatgtcgat
     8581 caaggttcag ataaaacagt catgatccat ggcggatttc gagggaattg gaataacggg
     8641 atcgttacag aagaatacaa tgatttttat aaggccgggt ataatttatt atttgttgat
     8701 tccagagcga ctggtaatag cggaggcgat tatgtgacct atggacaata tgagtcagat
     8761 gacgtccttt actggataaa tcaagaagtt cgagagcggc catctcaaaa gattttactt
     8821 tatggtggtt cgatgggagc agctacgatg atgtctgtat tagcgaaaga tattcctgtc
     8881 aatgtcaaag gaattattga aaattgcgga tttgcatcaa ttgatgaaca actacgtttt
     8941 acgtacagcc agacagtagc ccccgcacta ccggatgcca taaaaaatca gttagacatc
     9001 attggggatc aagagcatga agatttattt atgggccttt tgaaacaata ctattttgat
     9061 caggagatgc atttggatac aaaagctgct ctacctacga ttggaatgtc agattcattg
     9121 cctaaattga ttatccatgg aacagcagat gatgtagtac ctgtgtctaa cgcgcaaaaa
     9181 ttatatgagt tatcaggagg gtataaagat cttttattag tagaaggagc agggcatgga
     9241 aaggcacagg aagttgacca tgctgcctac acgaaacatg taacggactt tttgaaagta
     9301 gtgttccatg atcagataaa cgtaaagtat gtggatgaaa acaataaaag tctactcaaa
     9361 gaccaggacg aaatccagct ttacggagct tatggagaaa attatatgac agagcaaaag
     9421 acattcgaag gatacgaatt agcaaatgtt gaaggaccga ctgaagggat ttttaatgag
     9481 acgataccaa ctattatttt ctattacaaa aaaatcccag tagctccacc aaaaaagcaa
     9541 gatccgacag agaatgcaga caataaggga aagaatgaac ctcgagagga tcaatcaagt
     9601 gatgatacgc aaccgattca gatgacagtg aaagtacaaa aaaagacaga tgcaaaaaaa
     9661 agaatgttgc cgaaaactgg agaaaagaaa cattcgcttc tcatttattc aggtggattt
     9721 ttatctgtta gcacgattat cacatggatt tggcgaagaa agagagggta aatgaatgaa
     9781 atggttcaag cgaatagtga ttgcagtacc agttatcatc gtagttattt tagtcactgt
     9841 atttttgatt aatcaaatca caccaaagcc gattagcttg cttgttagaa aacaatttga
     9901 cacaagtgaa aaagaacaag tatatgcccg accagatgat ttccagcaaa aaatcgaaca
     9961 aatcgatata aaaaaagaaa tatcctatcc ttcaaagtac aaaaataata atatggatat
    10021 ctacacaccg aaagtggaaa agaaaaaatt accgattctt ttctggatgc atggaggcgc
    10081 atatgtagga ggggataaaa atgattgtcg tgattatttg gaatatctat gttcagacac
    10141 gcagcaaatc atcgtaaata tagattatga acgatcaccg gaagcaaaac atcctacacc
    10201 agttatccaa ttgaacgaag ccattcaagc agctaaaaaa gagataaaag atcaagcaga
    10261 ttggtcgaat atttcgattg gtggaaattc ggcaggtgca caaattagtg gagaatattt
    10321 gttagcactt caaaatgaag aaattaaaaa agctgatcat cttgatccga ctttggagaa
    10381 caagcagatt acgaagttta tttctttgtc tggtctgttg gatccttctg cacttactca
    10441 agtaagtgat aaaatctcta gctttcttta tgaaaaatgt ggatgggctt actttgataa
    10501 taaggacttt gaacaatcag agaaggtcaa aagtttagcc ttggtcagac atgcggatcg
    10561 ttggacgcaa aacacattct taacggatgg aaatacaaat acgtttacga aacaaatgga
    10621 agaagtaact tctgtatttg agaaagcggg ggtgaaagtc acaaaagtag attatgcgga
    10681 gaaaaatgct gttttgaacc atgaatatca gtttgatttc agcaataaac aagcgcaaga
    10741 aacatatcaa aagttagtaa tgtttttcaa ggagtaaagt cttatgatgc aaaagaggtt
    10801 gtaatctgtc acttagctcc aagtcacaag gagcaatttc aataaacaag cttttcatgg
    10861 tttatgaata ttgtaacttg tgactgagga gttagagatg gcaaggggca ccttttattc
    10921 gaaatttggg aatatcacac gattattgtc acgatctctt ttacgtaaat tttataagtc
    10981 agatttgttg ttttgatcgt ttaaaaaaag caacaaatcc gaagcctgcg gaggcaagaa
    11041 gggacaaaaa gccgagtggt aagcaaaaga atgctggttc cactaatatt ccgttgttat
    11101 tgatataagg ctgggaacaa agatgaatag caaataaaat caatgcacta gcgaaaaaaa
    11161 gcaaagaaat atggatagct ttcataagca gcacctcctt ttttataatt gtatgataaa
    11221 tagattaaga atctataaat gtgacaagtt tgttaggatg tgaataaaat gatcatctag
    11281 cgaacttgaa acaaagatgg ctaatgataa gcaattaatg attcagcaat cagataggta
    11341 caaagtttac catcatacgt ctagaccaat aatagaaaat atgggaatat aatagaaaag
    11401 atttaggata cccccaaaag aatttcccca aaaattcgat gtatcctaaa cttttttatt
    11461 ttacataaca atcatatcaa ctgtgaaata aaattacaaa tgataaccta aaatgaggcc
    11521 aaaagccagt gttatcaagg aaatacttaa ttcaccaaaa ttttttttaa ttttttagaa
    11581 aataagaaac aaaaataata aaaaaaagaa aagataaatt ttttactact gtatactaac
    11641 tttgttatcg catctaacaa gtaactcgaa taataccaat acaaccttct ccaaagatgc
    11701 gatgacatac gttctaaccc accgactatt gatgttctgt accacccttg cagaaccaaa
    11761 aaagccacta ccaaatattt ggtagtggct ttttttgttt tatggatttt tcgctttttc
    11821 tgttatggtg ctattctttg atttcagaaa tagtatgtcg aactttagcg atagaacgac
    11881 gtgattcttt ttcgttgtaa aacatcaaaa tcacatatag agagtccaaa atgcttagtt
    11941 gagaaatacg ggaagctaaa gcttcagatc ggaattctgt ttcttcagaa atagaaataa
    12001 aaacgacatc gccaagttta gcaagggaag aattagcttg gctagtaata acgattgttt
    12061 ttgctccagt ttttcttacg gtttcagcaa tatggattgt ttcttttgat tgccctgtgt
    12121 gagaaatcaa aacagcacag tctttttccg tcaataaaga agcttccatc aattgaatat
    12181 ggtaatccgt gctgtgagaa gttgcgatag gggaacgcaa aaatttatga taagcgtcag
    12241 ttgctacaat ctctgaaccg ccaacaccga aaaaatagac acgtttagat tcattgataa
    12301 tagtagccgc tttttttaga tccttttgtt gtaacaactt ttttgtgtca tttaaagttg
    12361 aaatactaga ctcgaacacc ttttgcgcca tagtgagctc agtatctgtt ttctgaatat
    12421 tttcgtggat agaaatagca gatagatcat tttcttggat caacatggcc attttaaaat
    12481 ctttaaatcc attataacca agtttttttg taaattgaaa aaaagtagag tcagcaatgc
    12541 ccagttcact tgctaaattg ctaataggac tatgacttac tttgcttgga ttttctaaaa
    12601 tataatcggc gatagcctgt tcttttgggc tgaagtcgtt gtacattgtt ttgatttgta
    12661 gtcttatgga gacgctcatg agaattcctc attttcataa taagattatc attttttccg
    12721 ttctcattaa tgttaaagat acaaggagag taggaaaaaa tcaaacaaaa aatatttttg
    12781 taaaatctat ttacaaaaaa atttttttgt ataatgaggg tataagaaat ggaggcgcta
    12841 tcatggaaat tggtgtaatt ggtttgggaa aaatgggatt gaatttagct ttaaacttaa
    12901 aagagcacca tcacaaagta caaggactcg acatttcaag tacagctgtt agtcaggcaa
    12961 gagaaaatga cattcctgct tatcaagaga tctctgaatt tctagctact tttgagcaga
    13021 aaaaaatcat ctggttaatg cttcctgctg gagagataac cgaaaacatg ttaagaaaac
    13081 tttttccaaa actcggacca ggagacatta ttattgaagg cggaaactct tattataaag
    13141 attcaatccg tcgagcagaa gagtttcaga aaaatgaaat cggttacttg gattgtggga
    13201 cttctggagg aatcgaagga gcacgacatc aagcctgctt aatgattgga ggagataaag
    13261 aaacttttct atccgtggaa caattattca aagatgtagc aattgaaaga ggatatctgt
    13321 atgctggtgc atccggaagc ggacacttct tgaaaatgat ccataatgga atcgaatatg
    13381 gaatgatgca agcaatcggt gaaggattcc aacttgtaca aaaaagtgag tatgcttttg
    13441 acttggaaca agtcgcacgt gtttggaatc atggttcggt gattcgcggc tggctgatgg
    13501 aaatcatgga agatcagtta aacgaacatc ccaatttaag taattatcgg ggaatcgttt
    13561 ctgcatcagg tgaagcaaaa tggacggttg aaacagcgct agaaatggct gttccagttc
    13621 cagtaattgc aactagtttg ttcatgagga atctttcaca ggaagaagat agtttttcag
    13681 caaaagtagt ttcagcacta agaaatgggt ttggcggaca tgaaatagtt gaaaagggtg
    13741 agtaaaatgc cagattatca tatagcaatc gttaattcca gcagtttcgg aagagtcttt
    13801 ccacaccact tggaaagatt aaagtcagtt ggagacgttc atttctttac tgttgatcaa
    13861 gagattccag gaaaagaact agcggagtta cttaaaggat tcaatatcat tattgcaagt
    13921 gttacgccat tttttcgacg agattttttt gaaaacaaag atgaactgct attgatttcc
    13981 cgacatggta ttggttacaa caatattgat ttaaaagcag caaaagaaca ccagacaatc
    14041 gtatctatca tacctgcttt agttgaacga aatgctgtag cagaaaataa tataactaat
    14101 ttgttgtcca tgcttcgacg cacgacacaa gcacaagcaa gagtcaagga cgatcgatgg
    14161 gaagatcggg cggagttcgt cggtaggacg ctgtttaaca aaacagttgg tgtgattggt
    14221 gcaggaaata ccggaagcgg agtaatcgaa attttgcgta acggttttcg ctgtgatgtt
    14281 ctagcttatg atccttacaa atcagattta tacatcgaaa gctttggtgc aaaaaaagta
    14341 gaacttcaag aattactgga acaatcagat atcatttgtt tatgtgcgaa tttgacagaa
    14401 gagaactatc acatgatttc tgataaagaa gtcgatcaga tgaaagaaaa tgtttatcta
    14461 tctaatagtg caagaggcgc tctagttgat gaagaagcga tgattaatgg tctgaaaagc
    14521 ggaaagatcg caggatatgc cacagatgtt ttagaagaag aaccgggacg tcaagatcat
    14581 ccgtatttag cgtttgaaca agtagtgatt acgccacata tatcggccta cacaagggaa
    14641 tgtttagaag caatgggtga aaaatgtgtc agagatgtcg aacagatcag tcagcacctt
    14701 cttcctgaac gttctgtcca gacaaagagc aactatataa aaggatgatg aagaaaatgg
    14761 tgaacggttt aaaagtgaaa acaggccctc aattttattt atatgaagaa ggtggtattt
    14821 caaaagtttc tgatctcttg aaatcatacg gcgcgaaaag agtactggtc acccacggaa
    14881 cggtatcatg ggaaaaggcg ctacctaaat tagtattttt gaatgacgaa acgattcaat
    14941 ttttttatca tcgttattct ggagaatgta gttatgctga agccagacga atagctacaa
    15001 tcatcaaaaa aatgaaatcg atttcgtagt tggcgttggc ggaggaaaac tgaccgatct
    15061 agtgggttat agtgccaatc ttgcaagaat cgatttcgga gtcatcccta ctttagctag
    15121 taattgtgct ccatggacac ctcttgctgt catgtataag gagaatggac aagcagaagg
    15181 gaaaacagaa cacttttaca gacaagctgc tttttttata acagatcccc aattagtgat
    15241 tgattcccct gttccatatt tcattgccgg gttagcagat acactagcaa aatggtatga
    15301 atctgagctc atcttagaac aagatttttt gcaagatgaa tcatttttga agctagcaca
    15361 agatactgcg aagatctgca aagaagagat tttgaatcat tctgctaaag caatccaaga
    15421 tatggcacaa agaaaactga catcggagtt caaacagctt tcagagatta tctttgctgt
    15481 tgctggttta gttggaggat taggagataa atacgcaaga aatgtggcag cacatgctat
    15541 gcatgacgct atcagtaaat atattccgga aagccatcgt tttctacatg gagaaaaagt
    15601 agcgtatggg attttctatc aactggcact agaaggtcgc tgggaaaaaa tagacgagtt
    15661 atatcctttg tatcgggaat tagaactgcc ggtttctcta gaacaaatgg gattatttcc
    15721 gaaagataaa cagataatag ataaaatggc tgaatttatt gattcgaaag agaaagtgca
    15781 tttgattcct atagaggtaa cacagaaatc actgatacag tcaatgatgg aactggaagg
    15841 ttatatcaga aagatctgaa ggaggaggac acatggaaac attaagtgta gtacaaagtt
    15901 tgttgattgc tttatgggta gccgcggtta tgtcccgctg gttaggtgga ggagccacgt
    15961 tgaccttacg tttttctcct ttgatgactg gattagtcgt cgggttgatc atgggggaag
    16021 ttgcacaagc gatgattgtt acagccgctt tgcaaatgat ttatatgggg gttttttctc
    16081 caggaggatc aatgccagct gaaccatcta ttgcagcagc tatcgcagta ccagtcgcat
    16141 tattagggga tctgtcgcca gaagcagcga ttgccgttgc ggtgccagtc ggattgctcg
    16201 gaagttactt gtatcagttt cgttttttca tcaatacttt tttaggtaaa tatacagaca
    16261 aagctgtaga agatttgaat acaaaagcta tggcccgatc aattattctt tatccaacgg
    16321 ttgcctcatt tttattattc gttccgcttg ttttcattgc tttatattta ggggcgccat
    16381 tgatcgctga tgtaatcaaa gctttagagg gaacagtcgt tatccacatt cttgaagtag
    16441 taggtggagg gttagcggca atcggtatcg cgacaactgt atacgttatc ggtcgaaaag
    16501 attatctggt ctttttcttc cttgcttatt ttatgagtgt tgttttgaaa tctttagata
    16561 ttacgatggt tacttatgcc atttttggga ttattatcgc tttgatcttt gtccagtcgc
    16621 aaaaagggaa acaagtacag cctgccacaa gcaataacaa ttcagcatct tttgatgacg
    16681 aagatgacta tgatgacgga ttttaattta ggaggaatgg gaaatggctg aaataatgga
    16741 taatcaaaca ggtgctcctg aagaaatcac gaaaaaagat gtaacaaaag cctatctgag
    16801 atggcatttt gcaaacgaaa ttcctcactc ttttgaacgt tacttggcgc catcactact
    16861 atacggaatg atgccaatct tgaaaaaact ttataaagat gaagactctt taaaagcagc
    16921 atataaacgg cagttactgt tcttcaacac tcaattaagc tggggtggcg gtgtgattac
    16981 tggcctaatg tcttctatgg aacagcaaag agcagaagaa gagtatgaag aaaaagagat
    17041 cttgatgcag gatgacttga tgtataacac gaaagcggga ttgatggggg cactagcagg
    17101 aatcggagat gcaatcgatt caggaaccgt ccaatatatt tttatcgcaa tcgcggtacc
    17161 ttgggcacaa cagggaagtc ctttaggcgc actttttcct tttatctgct ttgggttgta
    17221 tcaggtttta ctaggggtat ttttcgcaag acaagctttc actatgggcc gtaatgctac
    17281 aggcctaaag caaaacactg gtgttcagac agcgattgaa atgctatcag ttttagggtt
    17341 gtttatgatg ggggtgcttg cgggaaacta tgtaaaagta gaatcttcat tgaaatttgc
    17401 gatatccgga cgtgagtttg ttatccagga aattcttgat caaatcgtgc cagggatatt
    17461 acctttagct gtcgtaatgg gtgtgtactt gttctatacg aaaaaaggat tgaaagtcac
    17521 acaagcatta ttatggctga ctggaatatt aattgtgcta gcaggaatcg gtattttata
    17581 aaaagaaggg aacgtgaata agatgggtgt agtcaattta gcgcgtgtgg atgagcgttt
    17641 gatccatggg caagtaatgg taacgttatc tcaaaaaagt ggagtgaatt ccatttttgt
    17701 tgtagacgaa gttgtagcaa aagataaatt tatgagagat ctttacaaaa atgcaggaag
    17761 ccgtacagga caaaaaacaa ttgtcattac accagagaaa gctaaattct attgggatga
    17821 atatcaattt aaagaataca attgtatttt gatcgcaaaa acagtccaaa cgatttacga
    17881 cttagtaaaa cacggaatcc ccatggaaga attgaatatt ggagggattg ctcaaaaaaa
    17941 tcctgataca gatcttttag tgacgaaatc tgtttattta aataaatcag atgctgaaaa
    18001 attgaaagaa atgaatgaac aatatggcgt aaagaatatt tattttcaag ccactccatc
    18061 tgcacctaaa acaggattaa aagatgtatt gaacaagttt gatttgtcat aaacaaatgc
    18121 ttagaaaaaa gataaagagg gcgaaaaaat gaagaagaac gagctgtttc gtgattggga
    18181 atttcgttat cgctatattt accgtaagcg ccgtacaaaa aagagtaaac agcgttttct
    18241 ttcagcatta gtatcagata tttacagtat gagaacagat gtgacagtca ttgcttacga
    18301 tacgctagcc taccgatcaa aaaatattta tgttggagat atcgaaaaag cagagaaagt
    18361 tatctgtact tattacgata cacccgttca tgcattagga agttatttca tgtttgattg
    18421 gaaagaccaa aggaaaaaaa caatctactc tatattattg tccttcattt tgttgttcag
    18481 tttaggttgg tggggaatga tgatatataa caaaaatcct catcacgtat tcgatctgct
    18541 atccgtataa acaggtatca ctgtactagc atttgggagc tactttttct tattgggaaa
    18601 agcagctcgc ggatggccta gcagacaaac ttttattcga aatacttcat ccatattaac
    18661 gatgctagaa atgatccgta cgattgatga tccgagtgta gcgtatgcgt ttgttgatga
    18721 aggctgttat ggagaaaagg gcttagacag cgtgagatcg ggtatgaaaa aagaggctat
    18781 tcttttttat ctagactctg ttggggcaga tacaccgcta cagttttcgg gaaattattt
    18841 ttcaaataaa gaacagtggc tgaaacaagt tgataagttg aaagaaaaaa acgtcaatta
    18901 tatttttagt gcaagaaaaa agcaagcgca gttcttctat ttgaccaaaa cagatttacg
    18961 agggaaaaca tttaattggc aaaatgccaa tcagattatt gctctttttc gataagtaaa
    19021 aagtgcaaaa caaggagtga gaagactaat gttggaaatc atgatcgtta cacatggtaa
    19081 gttgagcgaa ggactgaaag atgcggcgga agtaattgtc gggacatcga gccatattga
    19141 aacaatgtgc ttagagtctg gggaggacat ccaaacattc ggagagaagg tcaaagaaaa
    19201 attaactatg atcaacacag cagaacccat gctagtattt gttgatctaa ttagcgcaag
    19261 tccgtacaat caagcgctct tggcagtcaa tgaactgact gaagaaaaga aagcagccac
    19321 ttatgtcata gggggcgtga atctgccaat gttgttagaa gcaatcaatc atcagatgct
    19381 tcacacaccg atagaagaag cggtggaaca aattgtcaaa caaggaaaag ataatttaac
    19441 tgtttggcat gcttcagcgg aagaaaaaaa attagatgag gacgattttt agaaggaagg
    19501 ctgaaaagat gaagtgggga tttagatggt atggtgaaaa aggggattcg atcccattaa
    19561 aatatgttcg ccaaataccg ggaataaatg ggataattgg aactttgctg actaaaatgc
    19621 ctggagatgt ttgggagatt tcagaaattg aggagttgaa agcatctgtt gagagccaag
    19681 gactggaact gcttggaatt gaaagtgtag cgatccacga tgcaatcaag gcagggacag
    19741 atgaacgaga ttactatatt gataactata tacgaacaat caaaaacttg tctcgatgtg
    19801 gggttcgatt gatttgttat agcttcaaac caatttttgg atgggcgaaa actgatttgt
    19861 ttaatgaaaa caacgatgga agctattctc ttttatacga tcagaaaatc gtcgatcaga
    19921 tggagccagg agaaatgtat caattgatac acagccagtc aaagggattc aaattgccgg
    19981 gttgggaaga agaacggttg aaaaaattcg ctcagctgga aaaactgtat gaaggaatga
    20041 ccgaagaaga attatttgaa aatctgactt attttttaga aaaagtgatt ccagtgtgcg
    20101 aaaaagcaga tgtaaaaatg gctatccatc cagatgatcc gccatgggaa ctttttggtt
    20161 ttcctagagt gacgaaaaat cttggcgact tgaagaaaat tctaaatgca gtagattctc
    20221 catataatgg actaacgctg tgcacaggat ctttaggggc aaatccggaa aacgatttag
    20281 ttgaaatcat ccatgaggta ggttcgcgca tccattttgt ccacttcaga aacgtggagt
    20341 ttttaggaga aagaaagttc aaagaaacgg cacatccctc tacagaaggc tcccttgata
    20401 tgtatcatat tatgaaagcg ttggtgcaag aagggttcga cggtcctata cgccctgatc
    20461 atggtagaac cgtgtggggt gaagtggcta tgcctggata tggcctgtat gatcgtgcaa
    20521 tgggactcac ttacatgcaa ggtctatatg aggcgataag taaggaaaaa ccactgggag
    20581 agcattatga ataaaatcaa gctcttcatc tttgatatgg atggcctttt attagaaact
    20641 ggacgtttag cttatcgtgc ttatgtaaaa tcagcacaaa agtatgatta tgaaatgcgt
    20701 aaggaagtct attatttact aactggccaa acagaaatgg cgataagaga acaaatgggc
    20761 attttgtacg gagaagatgt tccatatatc aaatggcgag aagcaatcaa tcaatataaa
    20821 gaaaaaatcg tgaaagaaga taagagagta tatacgaaaa aaggagcaga agaaatttta
    20881 tcttttgcaa aagaaagagg aatccataca atcgtggctt cgtctaatac acgggtaaaa
    20941 gtagaaatgt atttaaaaat ggaaaatcta tatgccttgt ttgatcaaat catttcaggg
    21001 gatgatgtaa aaaaaggaaa gccggaacca gagatctttt tgaaagcttg ttcaaaaatg
    21061 aatattcctc caagtgaagc acttgtattt gaagactcga ttgcagggat cgaagcagca
    21121 aggcgagcgg gcattttgtc ttttctcatc aaagatcatt tgatagggtt gcccgatcat
    21181 aatgggaagc ataagttgaa agtagatgta agtttatatg aatccaataa agcagactat
    21241 gaattttatg acctgcatca agcaaaacga tttttagaaa gtaaagggtt gtatctctaa
    21301 taaagaggac tattttctag tcctctttat ttattgtcct gcacgactga gggaacagat
    21361 gaaaaagaag taatttttta ttttgaagta tctaccttat ttgtagtgaa caatttagaa
    21421 caaacacaaa acgcatctac tgttatctac cagtagatgc gtttaaagag aagaacgaga
    21481 atcgtccata tgagggtttc c
//
LOCUS       DAFEOM010000039        21371 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.040, whole genome shotgun sequence.
ACCESSION   DAFEOM010000039
VERSION     DAFEOM010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.040"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            131..505
                     /locus_tag="LP524_001695"
     CDS             131..505
                     /locus_tag="LP524_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBL2234752.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="LP524_001696"
     CDS             complement(619..1248)
                     /locus_tag="LP524_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBL2234753.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="LP524_001697"
     tRNA            complement(1382..1454)
                     /locus_tag="LP524_001697"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="LP524_001698"
     tRNA            1668..1741
                     /locus_tag="LP524_001698"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="LP524_001699"
     CDS             complement(1879..2043)
                     /locus_tag="LP524_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234754.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="LP524_001700"
     CDS             2338..3666
                     /locus_tag="LP524_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HBL2234755.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="LP524_001701"
     CDS             3868..4701
                     /locus_tag="LP524_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBL2234756.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="LP524_001702"
     CDS             4717..5454
                     /locus_tag="LP524_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HBL2234757.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="LP524_001703"
     CDS             complement(5609..6577)
                     /locus_tag="LP524_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBL2234758.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="LP524_001704"
     CDS             6809..7642
                     /locus_tag="LP524_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBL2234759.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="LP524_001705"
     CDS             7676..8515
                     /locus_tag="LP524_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL2234760.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="LP524_001706"
     CDS             8541..9101
                     /locus_tag="LP524_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBL2234761.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="LP524_001707"
     CDS             9300..11021
                     /locus_tag="LP524_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HBL2234762.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="LP524_001708"
     CDS             11186..12328
                     /locus_tag="LP524_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBL2234763.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="LP524_001709"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="LP524_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBL2234764.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14128..14775
                     /locus_tag="LP524_001710"
     CDS             14128..14775
                     /locus_tag="LP524_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBL2234765.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    14904..15120
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15168..17813
                     /locus_tag="LP524_001711"
     CDS             15168..17813
                     /locus_tag="LP524_001711"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBL2234766.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            18123..19436
                     /locus_tag="LP524_001712"
     CDS             18123..19436
                     /locus_tag="LP524_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBL2234767.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            19426..20079
                     /locus_tag="LP524_001713"
     CDS             19426..20079
                     /locus_tag="LP524_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL2234768.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            20134..20805
                     /gene="radC"
                     /locus_tag="LP524_001714"
     CDS             20134..20805
                     /gene="radC"
                     /locus_tag="LP524_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBL2234769.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     SEDSL"
     gene            complement(20700..>21371)
                     /locus_tag="LP524_001715"
     CDS             complement(20700..>21371)
                     /locus_tag="LP524_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBL2234770.1"
                     /translation="NKNYCPPIIDKKLFYAVQKALPKNLKTNAKRDYIFKGLLKCSDC
                     QGSVAGQTIKARYKKKDGTESIYERTCYRCVKRRNNKLRCTNKRAFYEKNLERYIFEA
                     TKQKFEQIQINYSKKQPKIIKKKNSKKNIENKLDRLKKAYLNEVIDLEEYKKDREALM
                     KELNEIEVEPAKIDIKNVEFILSKEFDEIYKESSEEEKNALWRSIIDNIIVFPDGNIT
                     VNFLI"
BASE COUNT         7173 a         3559 c         4386 g         6253 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttgcga
    13921 aaaagctgcc ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatatttta
    13981 cgtttggttg agcttattac cgcagatgca agcttttgaa caccgtttta tttgtctcac
    14041 acccttttcc ttcctacttg taaccttgct cccgtgttag aaacatgtta cactaaatat
    14101 gtgaaattat taacaaggag cgtcgttatg cgagaaaaaa cagatacact gccaaaagca
    14161 actgcgaaaa gattaccttt atatttacgc tacttgaaga tgctggatga ctcaggtata
    14221 agtagaatca aatcaaatga gttcagcgaa atcacccaaa ttccttctgc aacgatccgc
    14281 agagacttct cgcaattagg cgagctaggc agaagcggat atggctatga tgtccctttt
    14341 cttatcgatg tattcaataa tatccttaat actaaagaag aaaaacgaat cgcattagta
    14401 ggatatggaa atcttggcaa agcattgaaa cacaacaatt ttagaagaaa tgaaaatcta
    14461 aatatcgtgt gcgtctttga taatgatcca gcactgatca atcgagtaat tgatggagaa
    14521 atgatctatc cgattgatcg tttcgcggag atagccaaag caaaaaatgt gacagtagca
    14581 atctctacgg taccaagtaa atattcacaa tctgctattg atgaaattgt caaaggaaat
    14641 gtaaccgcta tacttaattt tgcgcctgac agagtaactg tcccagctta tgtcaatgtt
    14701 caatacattg atttgacaac cgagttgcag acgcttattt actttgatga gaattactca
    14761 gaagtttttt cttaggaaaa tttgagtagg cttattgatc ttgcgagaac tcgtcgtaat
    14821 tctttcaaaa attttttagt actaaatgaa gtggtttttt cacgaccact tgttgactaa
    14881 aaaaaaaaac cattgtagaa tgaagcataa cgctgaacaa gaggagtaac tggcacattc
    14941 attcttagag agagaactat ctgctgaaag gttctcaatg acaaccagtg aaggtagctt
    15001 gggagtttct gtcttgaatg gagtagagat agacgagtcg tgatcgttaa ctcacttgag
    15061 aggcaataat gattgattat tgcaatttag gtggtaccgc gtaaatcata cgtcctagaa
    15121 tacgaaagta ttttaggacg ttttttagtt taaaggagga aaacagcatg acggaagaaa
    15181 ataacctatc aacaaaatat aatccgcaag aagttgaagc tggacgttat cagcattggt
    15241 tagaacaaga tttgtttaaa ccgagcggag ataaaaaagc aaaaccttat tcaatcgtaa
    15301 tcccgccccc aaatgttact ggaaaacttc atttagggca tgcttgggat acgaccttgc
    15361 aagatatgct gattcgtcaa aagagaatgc aaggatatga tactttatgg ctgcctggta
    15421 tggaccatgc aggaatcgcg acacaagcaa aagtagaagc gaagcttaga gagcaaggga
    15481 tttctcgtta tgatctaggt cgtgaaaagt tcatcgatca agtgtgggaa tggaaagaag
    15541 agtatgcgag ccacatccgc gaacagtggg ctaaaatggg cttatcttta gattacagta
    15601 gggaacgttt tacattagat gatggattat ctgaagcagt acgtaaagtt tttgttacct
    15661 tatacgaaaa aggtttgatt tatcgtggtg aatatatcat caactgggat cctcaagcac
    15721 gaacagctct atctgatatc gaagttattc ataaagatat cgaaggcgcc ttttatcata
    15781 tgagctatga actagcagat ggatcaggcg tagtagaaat tgcgacaacc cgtccagaaa
    15841 cgatgttggg agatacggcg attgctgttc atccagaaga cgagcgttat caagcgctca
    15901 tcggcaaaaa agtgattcta cctttagtca ataaacaaat cccaatcatt gcagatgaat
    15961 atgtagatat ggaattcggg acaggtgtag taaaaatcac tccagcacat gatccaaacg
    16021 actttgaggt aggtaatcgt catgatcttc cacgcgtaaa tgttatggat gatgatggta
    16081 cgatgaatga actagcagga aaatatgctg gaatggaacg ttttgctgct cgtaaagcaa
    16141 tcgtgcaaga tctgaaagaa atcggtcgat tgattaaaat cgatacgatg atccacagtg
    16201 tgggtcattc cgagcggaca ggtgtcgttg ttgaacctcg gctgtctact caatggtttg
    16261 taaaaatggc gccgttagcg aaaaaagcca ttgataacca agaaacagat caagcagtgg
    16321 aattcttccc tccaagattc aatcaaacgt ttttacgttg gatggaaaat gtccatgatt
    16381 gggtaatctc acgacaatta tggtggggac atcaaattcc tgcatggtat cacaaagaaa
    16441 caggtgaaat gtatgtaggg atggaagcac cagcggatag tgaaaactgg gtacaagatc
    16501 ctgatgtttt agacacttgg tttagttcag ctttgtggcc tttctcaaca atgggctggc
    16561 cagatacgga aagcgaagat tataagcgat acttccctac tagcacttta gtaaccggtt
    16621 atgacatcat tttcttctgg gtaagcagaa tgattttcca aagcttggaa tttactggcg
    16681 aacgtccatt tgagaatgtc ttgattcatg gattgattcg tgacgaacaa gggcggaaga
    16741 tgagtaaatc attaggtaat ggaatcgatc cgatggaagt aatcgaaaag tatggagcag
    16801 atgctctccg ctggttctta tcgaatggtt ctgcaccagg acaagatgtc cgcttcagct
    16861 atgaaaaaat ggatgcttct tggaacttta tcaacaaaat ctggaatgct tcacgtttcg
    16921 ttatcatgaa tatcgaagga atgaccgtag aagagatcga tttatctgga gaaaaatcag
    16981 tggctgaccg gtggatcctt actcgtttga atgaaacgat cgaacgagta actgagctat
    17041 ttgatcgttt tgaatttggt gaagcaggtc gacaactgta taactttatc tgggatgatt
    17101 tttgtgactg gtacatcgaa atgagtaaag aagtacttta tggagatgat gctgtttcaa
    17161 aacaaaccac ccgtagcatc ttagtccata cacttgatca gatcctacgt ttattgcatc
    17221 caatcatgcc attcgttaca gaagaaatat gggaacatat tcctcatcag ggaaattctc
    17281 tagtagtggc agaatatcct gttgtacgtc cagagtttga tgatgaaaca gcttctaaag
    17341 ggatggaagt attgaaagaa ttgattcgag ccgttcgaaa tattcgttca gaagtgaata
    17401 caccattgtc taaaccaatt acgttgctaa ttaaaacaaa cgatgctaaa atcgatcaat
    17461 ttttagttga aaatacaagc tatatcgaac gcttctgtaa tcccgaagag ttgacgatat
    17521 caagtgagat cactgcgcca gatctagcga tgtcagctgt tctgacaggt gcagaaatct
    17581 tcttgccgtt agcaggattg atcaatattg aagaagaaat caaacgatta gaaaaagaac
    17641 tggaaaagtg gacaagcgaa gtaaaacgtg tccaaggaaa actttctaat gaacgttttg
    17701 tttctaacgc gccggaagaa gtagtagagg cagagcgtgc aaaagaaaaa gattatttag
    17761 agaagcaaac ggctgtcaaa gaacgaatcg aaagtttgcg tactattcaa taagaataga
    17821 aaaagagagt gtgaaatcag ctagctatta ctaaaagcta gctgattgaa catcgttaca
    17881 ggaaagagtt tgagcctcac tcttagcttc gattcataag gagatgtgac ataaattttg
    17941 tcgcatctct tttttagtct actcttatta tgtaggagaa ggagggaggt ggatcatgta
    18001 tcaagagtca ataaagtagt tgataaaatc gttcctctct ctgtttcttt tctctttgtc
    18061 atacttttat ttttcttatg tgttctattt gttataataa aagagaaatg ggggaattgt
    18121 tgatgacgat tgaagaagca atagaatgga tccatagtcg attaccattt ggttcacgtc
    18181 ctggccttga ccgagtcgaa gctttattag aagtggtagg gcatccagag agaaaagtaa
    18241 aaacgatcca tatcgctgga acaaacggaa aaggttctac tgtagcttat ttgagaagcc
    18301 tattagaaga ggctgggctg acagtaggta catttacttc accttatatc gaaacgttca
    18361 atgaacggat cgcaatcaat ggcaaaagca ttccagacaa tgatttgatc cagcttgtaa
    18421 aaaaatatca gccactgatc aaacaacttg atcaatttga agcagtagct ggtattactg
    18481 aattcgagac attaacagca atggcatttg attatttttt gatgaagaaa gtggacgtag
    18541 cgattatcga agttggctta ggcggcttat tggattctac caatgtagct gtaccaatgc
    18601 tgacgggaat aacaacgatt ggacttgatc ataccgaaat acttggagat acaattgaag
    18661 aaatcgctgc acaaaaagct ggaatcatca aacaaggcgt tccagtagta acaggaaata
    18721 ttgccccaga agcttttact gtcatccaac aaacagctaa agcaaaaaat gcaccgcttt
    18781 atgtatacgg ggaaaattat aaaataaact atctgcatcc tgatgagatg tggggagaag
    18841 tatttgaatt ttttaatgaa ataggaaaga tccctcaatt aaccactccg cttttagggc
    18901 aacatcaagc tgaaaatgca ggagtagcca tcgaattgta ttttgtttac tgtcaattgg
    18961 aaaagctgcc tttccgagaa aaagatgttt tgaagggatt gaagaaatct cgatggccag
    19021 gaagaatgga gcgtttgagt aacgagccat tagttgtttt agatggtgcc cataatacgc
    19081 atgcaatgaa ccggcttgtt gagaatttga aaaaagaatt caaaggatat caaatcaaca
    19141 ttttgttttc cgccttacga actaaaaatg ttagagaaat gctagaacga ttgcagcaag
    19201 taccaaatgt ccatatttat ttgacgacct ttgaacatcc aaaagccatc gaattaagtg
    19261 aagtatcaga tttagcaagt gagagagtat caatcgtttc attgtggcaa ttcggattag
    19321 cagatatatt ggaaaagatg acaaatgaag acgtattagt tgtgaccggt tcactttatt
    19381 tcatttcaga agttcgccat ttattaatac aattaggagg agatcatgaa gtataatgca
    19441 gtgatttttg acatggatgg tttgctgttc gacacagagt tagtgtatta cgaagccaca
    19501 caagtagtag cagacaagat gggttttcct tatgataaag acctgtattt aaaattctta
    19561 ggtgtttcag atgaagaagt atgggcgaat tatcatatga ttttctcgga atttggcaaa
    19621 acaagtgtac aaagattcat agatgattca taccaggaaa cgcttgaacg tttttcgcaa
    19681 ggagcggtac aaatgaagcc cggtgtgttg gaactcctct cttttttaga agaacatcag
    19741 attcccaaag ttgtcgcttc cagtaaccaa cgagcagtca ttgaactttt attgaagaag
    19801 aacaatctgt ttgaacgatt tgagacgatt gtttctgcag aaaatgttca acgagcaaaa
    19861 ccagacccag aaattttctt gttagctaat cagaagctag gtacagcgaa gaaagaaaca
    19921 ttgattttag aagattcaca aaatggtatt ttagcagctt ctgcggctga gatcccggta
    19981 gtcatggtac cagatctgct tccaccatcc caagaattga tcaaaaaaac agctggggtt
    20041 gtttcttctt tacatgaaat ccctgcattg ttaaaataaa aatccatctc ttttcgtatc
    20101 ttattttaga taagatagaa aggagatgtt tttttgggaa aacgattgat caaagaagta
    20161 cctactagct ctttaccaag ggaacgtatg gaaatttatg gcgcggaagc tttatcggat
    20221 caggagcttt tggcaatctt actgcgtacc ggtcaacacc cttatagtgt gatgtcgatt
    20281 gctggaaatc tgttaaaaac gtttggagga ctggcctctt tacgacaggc tactttgcat
    20341 gagttagaag agattcgagg gataggacgc gtgaaagcaa tcgaaatcaa agccttgatc
    20401 gaattaggtc gacgaattca gacggatcat gaaagagcgg ctccaccggt tcgtaccagc
    20461 tacgaattgg cccagcaatt gattctggaa atgaaagatc acaaacaaga acatttggtt
    20521 tgtatttatt tggacacgaa gaatcaagtg atcttaaaaa aaacagtgtt catcggttcg
    20581 ctgaatcagt cagtcgctca tcctagagaa attttccatt atgctgtacg ttattgtgca
    20641 gcaaggatcg ttttagcgca taatcatcct tccggaatat ataagttagt acacataaat
    20701 tatataagga aatttactgt tatattgcca tctggaaata ctattatgtt atcgataatt
    20761 gaacgccata atgcattttt ttcttcttcc gaagattctt tataaatttc atcgaattct
    20821 tttgatagaa taaattcgac attttttata tctattttag ctggctctac ttcaatttca
    20881 tttaattctt tcattaaagc ttctcgatct tttttgtatt cctctaagtc tataacttca
    20941 ttcaagtagg ctttcttcaa tctatctaat ttattctcaa tatttttctt actatttttc
    21001 tttttaataa tctttggttg tttttttgaa tagtttattt gtatttgttc aaatttttgt
    21061 ttagtagctt caaaaatata acgttctaaa tttttttcgt aaaaagcgcg cttatttgtg
    21121 caacgtaatt tattatttct tcgttttaca catctataac aagtcctttc gtatatagat
    21181 tctgtaccgt cttttttctt atatcttgct ttaatagttt gaccagctac tgatccctga
    21241 cagtcagaac attttaataa acctttaaaa atgtaatcac gttttgcatt tgtttttaaa
    21301 tttttaggta aggctttttg aacagcataa aaaagttttt tatcaataat aggaggacag
    21361 taatttttat t
//
LOCUS       DAFEOM010000040        20942 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.041, whole genome shotgun sequence.
ACCESSION   DAFEOM010000040
VERSION     DAFEOM010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.041"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            169..363
                     /locus_tag="LP524_001716"
     CDS             169..363
                     /locus_tag="LP524_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234771.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            632..930
                     /locus_tag="LP524_001717"
                     /pseudo
     CDS             632..930
                     /locus_tag="LP524_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1027..1461)
                     /locus_tag="LP524_001718"
     CDS             complement(1027..1461)
                     /locus_tag="LP524_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2234772.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="LP524_001719"
     CDS             complement(1693..2403)
                     /locus_tag="LP524_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBL2234773.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="LP524_001720"
     CDS             complement(2393..3253)
                     /locus_tag="LP524_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HBL2234774.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="LP524_001721"
     CDS             complement(3258..3896)
                     /locus_tag="LP524_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBL2234775.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="LP524_001722"
     CDS             complement(3911..4210)
                     /locus_tag="LP524_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2234776.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="LP524_001723"
     CDS             complement(4253..5737)
                     /locus_tag="LP524_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBL2234777.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="LP524_001724"
     CDS             complement(5757..6218)
                     /locus_tag="LP524_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL2234778.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="LP524_001725"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="LP524_001725"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBL2234779.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="LP524_001726"
     CDS             7521..8291
                     /locus_tag="LP524_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL2234780.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="LP524_001727"
     CDS             complement(8564..9391)
                     /locus_tag="LP524_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2234781.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="LP524_001728"
     CDS             9959..11194
                     /locus_tag="LP524_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBL2234782.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="LP524_001729"
     CDS             complement(12193..12453)
                     /locus_tag="LP524_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBL2234783.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="LP524_001730"
     CDS             complement(12480..13781)
                     /locus_tag="LP524_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL2234784.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14073..14531)
                     /locus_tag="LP524_001731"
     CDS             complement(14073..14531)
                     /locus_tag="LP524_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL2234785.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14544..15875)
                     /locus_tag="LP524_001732"
     CDS             complement(14544..15875)
                     /locus_tag="LP524_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HBL2234786.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGRDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15886..17328)
                     /locus_tag="LP524_001733"
     CDS             complement(15886..17328)
                     /locus_tag="LP524_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL2234787.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(17315..18079)
                     /locus_tag="LP524_001734"
     CDS             complement(17315..18079)
                     /locus_tag="LP524_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HBL2234788.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18183..19058)
                     /locus_tag="LP524_001735"
     CDS             complement(18183..19058)
                     /locus_tag="LP524_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL2234789.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19313..19397)
                     /locus_tag="LP524_001736"
     tRNA            complement(19313..19397)
                     /locus_tag="LP524_001736"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19361..19363),aa:Leu,seq:caa)
     gene            complement(19415..19486)
                     /locus_tag="LP524_001737"
     tRNA            complement(19415..19486)
                     /locus_tag="LP524_001737"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19452..19454),aa:Gln,seq:ttg)
     gene            complement(19496..19568)
                     /locus_tag="LP524_001738"
     tRNA            complement(19496..19568)
                     /locus_tag="LP524_001738"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19533..19535),aa:His,seq:gtg)
     gene            complement(19630..19714)
                     /locus_tag="LP524_001739"
     tRNA            complement(19630..19714)
                     /locus_tag="LP524_001739"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19678..19680),aa:Leu,seq:caa)
     gene            complement(19735..19805)
                     /locus_tag="LP524_001740"
     tRNA            complement(19735..19805)
                     /locus_tag="LP524_001740"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19771..19773),aa:Cys,seq:gca)
     gene            complement(19851..19922)
                     /locus_tag="LP524_001741"
     tRNA            complement(19851..19922)
                     /locus_tag="LP524_001741"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19888..19890),aa:Gln,seq:ttg)
     gene            complement(19934..20006)
                     /locus_tag="LP524_001742"
     tRNA            complement(19934..20006)
                     /locus_tag="LP524_001742"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19971..19973),aa:His,seq:gtg)
     gene            complement(20009..20082)
                     /locus_tag="LP524_001743"
     tRNA            complement(20009..20082)
                     /locus_tag="LP524_001743"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20048..20050),aa:Trp,seq:cca)
     gene            complement(20089..20169)
                     /locus_tag="LP524_001744"
     tRNA            complement(20089..20169)
                     /locus_tag="LP524_001744"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20133..20135),aa:Tyr,seq:gta)
     gene            complement(20171..20246)
                     /locus_tag="LP524_001745"
     tRNA            complement(20171..20246)
                     /locus_tag="LP524_001745"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20211..20213),aa:Phe,seq:gaa)
     gene            complement(20282..20354)
                     /locus_tag="LP524_001746"
     tRNA            complement(20282..20354)
                     /locus_tag="LP524_001746"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20319..20321),aa:Asp,seq:gtc)
     gene            complement(20364..20436)
                     /locus_tag="LP524_001747"
     tRNA            complement(20364..20436)
                     /locus_tag="LP524_001747"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20401..20403),aa:Val,seq:tac)
     gene            complement(20452..20523)
                     /locus_tag="LP524_001748"
     tRNA            complement(20452..20523)
                     /locus_tag="LP524_001748"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20488..20490),aa:Glu,seq:ttc)
     gene            complement(20538..20627)
                     /locus_tag="LP524_001749"
     tRNA            complement(20538..20627)
                     /locus_tag="LP524_001749"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20591..20593),aa:Ser,seq:gga)
     gene            complement(20667..20740)
                     /locus_tag="LP524_001750"
     tRNA            complement(20667..20740)
                     /locus_tag="LP524_001750"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20704..20706),aa:Asn,seq:gtt)
     gene            complement(20750..20865)
                     /gene="rrf"
                     /locus_tag="LP524_001751"
     rRNA            complement(20750..20865)
                     /gene="rrf"
                     /locus_tag="LP524_001751"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         6601 a         4390 c         3332 g         6619 t
ORIGIN      
        1 ttaagaacaa tagcctttta ttatatatat tattcaaatt agttatatcg agtgaaaaaa
       61 actttcaaat tgattctttt tatttatcat aaagatatag atacataatc attttcactt
      121 acatatattt gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta
      181 gaagtaaaaa caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat
      241 cctttacttg gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata
      301 aagaacgagc tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag
      361 taaatccaat gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat
      421 tatagaatta gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata
      481 ccatatgatt atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat
      541 gaatcaaata tacttgtcat tcatcatgaa atatagagac ttataaaatt gtttattttt
      601 ataatgtatc agtatagaaa ggaaataaaa catggattat actgttgaat ttgacagtga
      661 actacttaag caatttccac aagaagaaca tttcatgtat aaagaagcaa tttcccacaa
      721 agtgacagag tatttggagc ataatttttt tcgtataaag ccagtgcgta ctcgttcggc
      781 aaaaggaata tacgagatga aagtcaaaat taataaaaaa aattatcgca tagcatttgc
      841 tttaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaagaatg ttcataacta attatataat
    13861 taagaaattt tatttcaata tattctctta aataatcaaa tttattttta agaatcctga
    13921 aaataagttt tctaaatcat aggagcaaca aaaaaattcg ataattaaca aaaatcaaaa
    13981 tgattaaatc tagtgattga aacatatcat ttcaaaaaaa ttttgtaaat tcacccataa
    14041 atatcaccct atttaacttt tttcatcgta cactaaaatt caaaacaact atctttaatc
    14101 tttacttctt ggttttgttt taataaattt ccttcaaaaa tgcctggaga aactacaatg
    14161 atcaccgtat ctttttgtgc ttgctccaac ttcttcaaat ctataatagc taacagtgtc
    14221 ccttctttta cctcctggtt actttcaacc cgtatctgaa acggacttcc ttcgagttca
    14281 actgtatcta aacccatatg gatcagtatt tttctgccag attcactttc gatcgttatg
    14341 gcatgtcggg taggaaatat attatcgatt attcctttaa ctggcgcatg gatatatcca
    14401 tcatgattcg ttatagcgta tccaactcct atcatttttg aagaaaagac ctcgtctttt
    14461 actgctgtta atggaataat ttgaccacta gctacagctt ttagaatgtt tcttgtttta
    14521 aagaaatgca ctttttatca cctctacttt aatgctggcc aatatttttt gtttactttg
    14581 atcatttcat ctagtatttt ttttgcgact ctggcatttg gtacggtttg attcaatgta
    14641 aatgcttgta aagctttttg ataagaacct tcaaagtaag catctactag taacttctct
    14701 actgcaactt gtgcctccat caatccttta tgaaaatcac tgattggttt tcttagtgta
    14761 attggctcca caccagttgc accaacatat gccggtattt caacaactgc atctgaccgt
    14821 agattcggaa tcgccccttg attaggaacg attagcataa agcgacgacg atcatcattc
    14881 aacaaggaaa tagctatatc gacgatatat tgtccatgct ccccaaaatt aaagttcaat
    14941 acttcttcca caataccgcg gcgtatttta tcagccattt ctttggtatt tttctctcgt
    15001 ccatccatga ccatattagc acgtgtatac tctttgtcag tatgctcaac gaccatatca
    15061 ggatataagt aatactctaa gtaattattc gggatattac ttggaaaatt tttcgtgatg
    15121 actgacatca tttgaaaggt tttttgccaa gttttatctc ctatattaaa atcggcgact
    15181 tgcatttctt ttgttattag tttttcaata atctctggca taatttctcg ttgcaattct
    15241 ttatcataaa tcgatgtata ccagccaaag tggtttaaac cataatacgt aacgatccaa
    15301 ttcttacgat cataaccgta attcactgca atcgtttctt caattgaaat agtcatatca
    15361 caggcattaa tcatttttac attcggatat ttacgacgaa cagcttcaga aacgatcgac
    15421 tctggatttg tataattcaa aatccatgct ttgggggcat agtcttgtat atgatcgaca
    15481 atttccaata aacctttgat tgagcgcatc ccataagcaa atcctccgag tccacacgtt
    15541 tcttgtccta ctaatccatg tttaagtgga atcttttcgt ccttttctcg catctccaat
    15601 cctccaacac gaatttgtga gaaaacaaaa tcgcgtcctg taaaagcaag ctttggatca
    15661 tccgttgcac gaatgcgaat atttgaaaaa ccttttcttt tcagcataaa tttaacaatc
    15721 aagtacatat ccatatttct ttcttcatca atatcatata aacgtatttc agataatggc
    15781 aaccgttttt cattgttcaa aatggcttga acgattcctg gtgtatacgt actcccgcct
    15841 ccagctatag tgataatctg ttttttcatc atcatctttt cctttctatt cttctaataa
    15901 tttattgaat tctgccataa cataagtcac ttctaagccg atgataattt gataattttt
    15961 ttcagttggt ttaatgatcc cgcttatccc taattgcttg attcgctgtt cctcgaccat
    16021 ggtgggatcc actacctcta cccgtaggcg tgtattacaa ctatagacat ccaggatatt
    16081 ggcttttcct ccaaacgctt gtagcatttt tgttgccata tattcataat ttttacttgc
    16141 taaccctaac tctgtttctt ctaaggttac ttcattgcca aactctgcct tagcttcacg
    16201 tcctaatgtc tgaatgtttc tcttattgat caaataataa aaagtaacgt aatacaaggc
    16261 aaaaaagaaa attccgattg gaagaatcaa aatagcattg gtagcgattc gaaaattgat
    16321 gagataatca acaatacatg ctccgaatgt aaaaccaatt cgtatattga ataagtaaca
    16381 gacaatccct gctaaaccgg caaaaagtgc atggatcacg tacaacatag gagaaagaaa
    16441 catgaaacta aattcaacag gctcagtgat atttgcaata aatgaagttc ctgcaccgct
    16501 tcccatgatt cctttgacca tctctttatt ttctttataa gctgctttat agatagctag
    16561 acatatagct ggtataccaa acatcattac tacaaaaaaa actgataaaa acagtccagc
    16621 ctgaggatct ccgttgataa agcgtgtcat ctctcctctg aaaacttcct tgctaggaga
    16681 tgtatagctt cctaaatcgt aataaatata cgtattcaac acatggtgca gtccaaaggg
    16741 gatcagcaaa cgattcaaaa acataaatat ccccacacct aatgctccca tatcaacgag
    16801 cagttttgca aaattatcaa tcccaatttg gatgagcggc cagataaagc ccaaaaaaac
    16861 agctgcgatt gtttgtagaa tcatcaccat agtcaatggg aatttttcgc ccgcaaaata
    16921 agaaaaaact actggtaatt tttgttcttt aaagcgatta tacgtccatg ccgctactaa
    16981 accggaaata attccaccaa aaatccccat attcacttct gggtccataa tttctaagcc
    17041 ttttttcaag gtagcaatcg ctaaaaaacc agtaagtgca ggtattcctt tgtccttatt
    17101 tttgacaaaa ccgattgcaa ctcctatagc aaataagata tccaagtttc caaaaacggc
    17161 atttccagct gcaccaatta ctgggatgtt cagcatatcg tcagctgata aacgcatgat
    17221 caatcccgct aatggcatcg ctgcaactgg aagtagcatc gctcttccaa gcttctgcac
    17281 atttgattta acattcattt ttattcctcc tctattaaat atcctcatat gttactaact
    17341 caatcaaagg atcatttttc actttttgag caaatgctga atgaattaaa aattcgactt
    17401 ggtgggtacg aggatacaca aaacttgaac catttaacaa ttcctgatcc aaatacgctg
    17461 cgtgacacat gacttcgatc gttttatctt ctctcaatat catgagtaaa cttgccaaat
    17521 attcttccac actcgtattg atttgtcgtt ttttttcatc agtttctcct acgacatcga
    17581 aacaaggctc aaaataatcg acatgacgaa cttcatcttt tcgttcaaag ttgctccgaa
    17641 ccggcaaatc atattttttt gctaatgtaa caactacctc ctgattttca tccaaagtat
    17701 gagtatgatg atgactgtct aaatgagttg gtatgatccc cgcatgataa attttttgaa
    17761 tctgagcatt ccattcctga tacagttgat ctttgtcaat gttaaatgga tgctgataat
    17821 aagataagga tctgaactct ccgctttcga taagagtatc gacagttttg aggagtggtt
    17881 tgccacacgt caatgttaaa tggactccta cacctagcct aggcatttcc tttcttagtt
    17941 tcacagcatg ctcgaaaccc ggcatatttg ccatcaatgt agttgatgtc aaaattcctc
    18001 gctgataagc atccataatc ccataattca caccatggct atagccaaaa tcatccgaat
    18061 taaaaatcac ttttcccatt tcattctcct tcttttttct actaaaatag gcgcaaccaa
    18121 ataatcatag tccatttact atgtgtcatt tggttgcgcc tatagatagt tacgccctta
    18181 ttttaataga ttcttgctag atgaattgcc aaatagatga tttcattttc ttgaatggca
    18241 agttgaaatt gctgttcgat gatatcttta atctttagtg ctgtacaata cgctttgcta
    18301 tgtttaaatg tttcagtaaa aaaaccaata ttttctactg gcgtttcttc ctgataaatc
    18361 aagcgtttaa ccaggaattt gatatgaacc attaagcgat gataagtcag tgattcttta
    18421 tcataaacgt ttgaatagga tttttctaga tgggacaaaa tcgtggtcgt catttctact
    18481 atttttaagc tctttaaact attaggctct cctagctcag cattaatgat atgcatagtg
    18541 atgaatccta cttcatttga ccctaactcg gtttgatatc gatgattgat tcgctcgacc
    18601 acttctactg ctatatcata ttcatttgga taaaggaatc tcatttctgg caacataatt
    18661 gctgtgacat ctctatcttc tctttttcgt tgaatagcaa aataaatatg atctgtcaat
    18721 gtaatataga tatttgaatc taatggagag gagatcctag ctgttatcag ttcaatcact
    18781 tcttccgcaa aatccataaa ttcgattgga atctgcgaga ctatctcact gaatcgtttg
    18841 atcttttctt tagaatctaa tacaaataat ttgtcagcat cctcaacatc aaatggctta
    18901 ctcactttag cagaaaagcc aatccctttc cccatcgcaa ttttttctat tccatcgtca
    18961 tcgatcaata cagcattatg attcagcttt ttcatcacat acaccgttaa ttcacctcct
    19021 ctatatgata aaacaaaaca acttgcagac tccttcatac atatttttgt ctccaaacaa
    19081 tttttaaaga caaaaaacga cctaagaaag aaccctttct taggtcgtgc ctaattgaat
    19141 agtagcaccc tacataattt acaaaaatag tttaacacat aataaaagcg ttatcaatat
    19201 cttttttact cgttttatga taatacttgt atcgaattga tttggaggta attacataaa
    19261 aacaaaaaaa atatagcaac tctctataag aaagttgcta tattgataat tataccggcg
    19321 gccggggtcg aaccggcacg tcctcaacgg acactggatt ttgagtccag cgcgtctgcc
    19381 aattccgcca cgccggcaaa tttaaaaata ataactgggg tagctggatt cgaaccaacg
    19441 catgagggag tcaaagtccc ttgccttacc gcttggctat accccaagaa taaagggcga
    19501 atgatgggaa tcgaacccac gaatgccgga gccacaatcc ggtgcgttaa ccacttcgcc
    19561 acattcgcca tcataaaaga tgttatttaa tttctcaata aaaaactaag atgaagacat
    19621 cttagtcaat accggcggcc ggggtcgaac cggcacgccc tcaacgggca ctggattttg
    19681 agtccagcgc gtctgccaat tccgccacgc cggcaagttg atttattagt actaaggcgg
    19741 taaccggatt tgaaccggtg atgaaggttt tgcagacctc tgccttacca cttggctata
    19801 ccgccattat actattatca atctataaaa taaagaataa attatagaaa ctggggtagc
    19861 tggattcgaa ccaacgcatg agggagtcaa agtcccttgc cttaccgctt ggctataccc
    19921 caagaataaa aagggcgaat gatgggaatc gaacccacga atgccggagc cacaatccgg
    19981 tgcgttaacc acttcgccac attcgccatg gcaggggcag caggaattga acccacacta
    20041 acggttttgg agaccgctgt tctaccttta aactatgccc ctataaaatg gaggagagtg
    20101 gattcgaacc actgaaccct aaggaacgga tttacagtcc gtcgcgttta gccacttcgc
    20161 tactcctcca tggtggcgcg ggacagaatc gaactgccga cacacggagc ttcaatccgt
    20221 tgctctacca actgagctac cgcgccatat tttaaaatta ttaattgaat aacaagaacg
    20281 acggttccga cgggaatcga acccgcgatc tcctgcgtga caggcaggca tgttaacccc
    20341 tacaccacgg aaccagttca ttatggaggt taacgggatc gaaccgctga ccctctgctt
    20401 gtaaggcaga tgctctccca gctgagctaa acctccaaca ttcaattaaa atgacccgta
    20461 cgggattcga acccgtgtta ccgccgtgaa agggcggtgt cttaaccgct tgaccaacgg
    20521 gccaggtaaa tttgctacgg agagtaaggg attcgaaccc ttgagacagt ttggcaccat
    20581 ctacatgatt tccaatcatg ctccttcggc ctctcggaca actctccaga gtgaaagaag
    20641 ctcaatactc tttctcaaaa tgagaactcc ggcagtagga ctcgaaccta cgacatcatg
    20701 attaacagtc atgcgctact accaactgag ctatgccgga ataacatcag cgtggcgacg
    20761 tcctactctc acaaggggca acccctcact acaatcggcg ctaagaagct taacttctgt
    20821 gttcggcatg gttacaggtg tatccttctc gctatcgcca ccacactgtg gtgttatctt
    20881 ttattgagta aattttgttc actcaaaact ggatttgaag tcacataagt ttttttccga
    20941 gt
//
LOCUS       DAFEOM010000041        20820 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.042, whole genome shotgun sequence.
ACCESSION   DAFEOM010000041
VERSION     DAFEOM010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.042"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            <1..189
                     /locus_tag="LP524_001752"
     CDS             <1..189
                     /locus_tag="LP524_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL2234790.1"
                     /translation="NSNGILRRNGLPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(1055..1585)
                     /locus_tag="LP524_001753"
     CDS             complement(1055..1585)
                     /locus_tag="LP524_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HBL2234791.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1582..2199)
                     /locus_tag="LP524_001754"
     CDS             complement(1582..2199)
                     /locus_tag="LP524_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBL2234792.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
     gene            complement(2805..3002)
                     /locus_tag="LP524_001755"
     CDS             complement(2805..3002)
                     /locus_tag="LP524_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HBL2234793.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYK"
     gene            complement(3180..4712)
                     /locus_tag="LP524_001756"
     CDS             complement(3180..4712)
                     /locus_tag="LP524_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL2234794.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            complement(4848..5933)
                     /locus_tag="LP524_001757"
     CDS             complement(4848..5933)
                     /locus_tag="LP524_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBL2234795.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            complement(5948..6667)
                     /locus_tag="LP524_001758"
     CDS             complement(5948..6667)
                     /locus_tag="LP524_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBL2234796.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            complement(6664..7974)
                     /locus_tag="LP524_001759"
     CDS             complement(6664..7974)
                     /locus_tag="LP524_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBL2234797.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            complement(8001..8591)
                     /locus_tag="LP524_001760"
     CDS             complement(8001..8591)
                     /locus_tag="LP524_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HBL2234798.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            complement(8909..9970)
                     /locus_tag="LP524_001761"
     CDS             complement(8909..9970)
                     /locus_tag="LP524_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL2234799.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     TCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQS
                     IIGVKTLAYLSGMGRNRRF"
     gene            complement(10538..11605)
                     /locus_tag="LP524_001762"
     CDS             complement(10538..11605)
                     /locus_tag="LP524_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBL2234800.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(11625..12104)
                     /locus_tag="LP524_001763"
     CDS             complement(11625..12104)
                     /locus_tag="LP524_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HBL2234801.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(12121..12582)
                     /locus_tag="LP524_001764"
     CDS             complement(12121..12582)
                     /locus_tag="LP524_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL2234802.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(12596..13846)
                     /locus_tag="LP524_001765"
     CDS             complement(12596..13846)
                     /locus_tag="LP524_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HBL2234803.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(14224..15675)
                     /locus_tag="LP524_001766"
     CDS             complement(14224..15675)
                     /locus_tag="LP524_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBL2234804.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(15996..16760)
                     /locus_tag="LP524_001767"
     CDS             complement(15996..16760)
                     /locus_tag="LP524_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HBL2234805.1"
                     /translation="MIDLHCHILPGIDDGAESLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(16788..17486)
                     /locus_tag="LP524_001768"
     CDS             complement(16788..17486)
                     /locus_tag="LP524_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HBL2234806.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(17498..18277)
                     /locus_tag="LP524_001769"
     CDS             complement(17498..18277)
                     /locus_tag="LP524_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HBL2234807.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(18293..19237)
                     /locus_tag="LP524_001770"
     CDS             complement(18293..19237)
                     /locus_tag="LP524_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBL2234808.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(19283..20062)
                     /locus_tag="LP524_001771"
     CDS             complement(19283..20062)
                     /locus_tag="LP524_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234809.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
     gene            20489..>20820
                     /locus_tag="LP524_001772"
     CDS             20489..>20820
                     /locus_tag="LP524_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBL2234810.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIK"
BASE COUNT         6640 a         4128 c         2696 g         7356 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatagattt tagagaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgtaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcatata atttaaaatt aacattgatt ctcctatgaa aatatctttt
      241 acctttctcc tgtgaaaagg aaatttcctt ttttatagtg ttattgatat aatcagaata
      301 ttcatcaaac tctgtatctg gagttaaaaa tctacagtaa catctataag ctttgctagt
      361 aatcttcttg ggttctctct ccattgtcaa gcaacatcaa aacacctact tactattcga
      421 ttcttttctg ccaaaaacga gtcagcatca ctcacaactt atgtgttgct gactccgact
      481 agtcctttgt tcaatatcaa cttattaaac agatataata tttttcttta tttcgtatac
      541 tccaagactt ccatctttct ttcatcttat ctctgaaaaa aacacagcaa agtgccctta
      601 ataaatatac attagaattt gcaaattttt tcatcttaag atatatccta ttcatattct
      661 ttacaatcct aatgggtgat tacaaatcta tttagttcaa gttctacatt ataaacacct
      721 ttcttgatag tatcatctgc tgcacaagcg atgtcgatcc acttattagg ggatttacgt
      781 gctcaaaagt ggctattcct tttgggaagt ccatccataa taatggtcca tggatttttt
      841 tagggtcttg tcgttcaaat ttctcaaaaa ctttattata cttccatagt tttataatta
      901 tttggtttca ctttggctcg ttcaaacacc aacttgtatt tcttttcaac gttcgaactg
      961 gagccccccc tataatctca ttattatttt catgactttt tactaccgtg gaattacaag
     1021 caacaacaat attatctgct aaatgaacac ctttctatat cacgcttttt gctccaatcc
     1081 aacaatgttt acctataact attttattat ttttattcaa aatcctatga gtattcaatt
     1141 caaaaatcgt atgtgaatcg gtatccataa ataatgtatc ccacgatact gtacagccat
     1201 ctttgaattg aatttcctta aagcaaatta tactcatttt tcctgtcgaa ttaaactttg
     1261 acccaatatc taaaactgca tttttcctta tgtcaatatg agccccttgt gcgataaacg
     1321 ctggcccttg tattcttagt attccttcaa tacttataat cgtttcttca tttcccgcag
     1381 tatttaatac tggaaagccc aaataaacta tttttccaga ttgtaatact atctcaattt
     1441 tccctctcga tttgcgtgta atccgtactt tacgatgcac caaaataggt aatctccacg
     1501 catctgaaat atgaagaaat ttataattga agtatatcgt tttaggtaaa tttaatatat
     1561 tctttattat caatccgagt ctcatctttc accatctctt tttgtaatca aaattagttt
     1621 ataaaggatt acacccaata taccgcccaa cgtattatat acaaaatccg aaatttgaaa
     1681 cgttcctagc ctcaaaaaaa gttgtaaaaa ttcaattatt gctgaaaaat aaaatgcaat
     1741 ttttcctcca tatactattg aggaccatat tttgggtttc tttatatttt catatttaat
     1801 agacattacc atataaacat atggaataaa aagaataata ttttctaaac acgctgtaga
     1861 taactctttt gttccagttg ttgtattata cttataaatc caccagtctc ccataacatc
     1921 agataaagga tttaaccaaa ttgttcgatt gattaatgtt gtaaataaaa tcatcgcagt
     1981 atagaatatc aaagcacaaa cttttctata ttttctatcc ttctttaaat gaagataaag
     2041 ttccattagt atacgtttat agccctcacc tgcagcaatg ccatctgttg catacataaa
     2101 atagaccagt cctaaaattg tcaataaaac agcaaaccca aagggttcat ataatttata
     2161 taatatatct gtaataattt ctgcaatcac tattatcata attatttctc caatatcaag
     2221 tatacactta tcgcttccaa ttacacatga tcatattaga gtgtattatc acattctcta
     2281 atattcaatt tatttttcct aaaatattcg aaataatcgt cttgataacg agtaagtatt
     2341 tttatacgat tgataacata gtaacaatat ttttaattat agtaaaaaat catctgcctt
     2401 tttcatccac ctaatcttat ttttcatttt attcaacatc cttaaataaa cttccgttcc
     2461 taagctctat aatatcttct taaaaaataa tattatggtg tcgttttcaa actcgtaatc
     2521 ttttacattg gaaaaataca tacttttctc aatgtagcaa ctcctatctc acagtattca
     2581 aaaattcttc aatattatga aatacttgtg catgttcttt aaataccttg tcagtctgtt
     2641 tataatcccc tactaaattt tgcattgctc ccaactcttc gacaaattgt catctccatc
     2701 tgtaaacagc cctaaaaact catataatgc tttttcatca atatttttcc catattattc
     2761 tcggtatagt ttcacaagtt ttacggattc ttcatattgt ttatctattt ataatattca
     2821 tctagggaca taattacttt tgctagattt cctgcaacaa cacaatcgtc tgatacgtcc
     2881 ttgtttacta aactatttgc acctataact acattatttc ctatatgtac accctttagt
     2941 attctagact tcattcctat aaaaacatta ttacctattt tgatcctctc cgatgatccc
     3001 aaagcttctc catactactt ttaaaaccga tcaatcataa acatgagtca aaattgtaac
     3061 tccttcatat catctccaat ttcaacaaga gtcttcgttg gaacaaaaac tatacatcgt
     3121 tcgcctattc ggcagcctaa aaattgtagt tactttatat atcattctga tgtctcttct
     3181 catcttttta ccaataccga cgtaagaact ttcaaaatat acttaaactt cttattacgg
     3241 atattaaaca gaagaataac taaaatcgat atgacaaccg atataactaa atttatcact
     3301 aaacttatca ctttgaaatt tccgatatta acatacgaac aagcaaaata acttaaataa
     3361 gaagcaacaa aagctattac acacaaactt atgagcttcc aaacatattt acctgccgat
     3421 tcaaatttaa atatattttg gaaaacatta tataataacc agggtaaatc aaccaacaat
     3481 actgaaacaa tggttgccgc caaaactcca tataatccga accaattaac gaggatgata
     3541 tttaatacaa tgtttatcat tgctgtaatt aatggtctaa acctgtctgc gtaccatatt
     3601 ccagcagcat ctttgtataa cccaattaac tgatcaattt catataagaa aaaatgaact
     3661 atcataaata taacaaatga aatactcata agattatcac ttcccaccca gataatcatg
     3721 aacggctgaa ataatgtaat aaagcatgca caactaattg ccagaaacca acctataatt
     3781 gttgtaatat cacaaaaatc tatataattt ttttcttgac tttctacaat aagactgtta
     3841 cctatacctg ctatacatga ttcataaatt attgcaaata tactcattat ggcagaaata
     3901 ataaagtaat aattctgata aatcgcaagt actgaaagac ctaagaaaga tgaaataata
     3961 attgaatctg aagatcgtaa tattgtaccg cctaccttgt ttgtaactaa tccttttact
     4021 ttttttataa tatcgtcaat agtttctttc tctaactcac caacaggagt ataatcagga
     4081 tacatctttt gagaaacaac agcacacatt aagttaagaa ctatttgtcc caaaatagaa
     4141 gccaataaat acaaatagta attttttaag aaaatcagaa catatgcctg cattacaaat
     4201 tggaatgtaa aagtaattag catagacacg ttaattacat catttctttg atgtgcatat
     4261 attagactct ttctatatga aaataaccaa tacgacaaaa cagtatttat caaatacatt
     4321 aaatacaaaa ctgtcatatt taaatttcca ggaatcgaac ctttaactaa aaatctcaaa
     4381 aaaggaatgc ataacaatcc taatgtaaac actatccatc caataattga aaagtacttt
     4441 ttatatgcgt tcagtaaagc acaaatctct tttctattgt cttctgctat aggtttatac
     4501 atacttacag ttagtgcagc acctattccc aattccgcta aatttaacat ttgaaatata
     4561 gatgtgaaca ggttgttcaa tccagcatac tccattccca tctgataaat cataagtgta
     4621 cgcataaaaa agggaatgat aatctgatat aacttaaaaa ttgttccagt aattatattc
     4681 cttttagcat tttgtttacg attaattatc atatcatatg gctccaaact ttctattttt
     4741 tattgttacc taatactata taatttctaa aatcttgaaa tgtaataggt ttcttctagc
     4801 tctttgatta attacgctaa ttttcatgtt tggacaacca caaaaaacta tacattttca
     4861 atcaaagcaa cccattgttt tgtaatttct ttcaaattaa atttttgtac attattatat
     4921 gcgttcagtg acattctcat tgctaaatca gtattcttag taaacattat catgctctta
     4981 gctaattggt ctatattctt atcttcaacc aacatcccat taaaattatg atgtaccaaa
     5041 tctctcggac cagttttaca atcataagaa ataactggta atccataaga ctgtgcttct
     5101 aatataacca taggaaatcc ttcataaaca gaactaagca cataacaaga tgcagattta
     5161 taatattcct ccacatggtc tgaatgccct gctatagtta ctctttgcag atttaaatct
     5221 tttatctgct ggcgaagatc tttttctaat tcaccttttc ctacaatgat aagatcccaa
     5281 tcatctactt ttttttctac aattttccaa gcacttatca tgtcagcaaa atttttctct
     5341 tccactaatc gccctactgc aagaaatctc tttttatcat aatgatattc gtactcgata
     5401 ttagatatac acggatttgg catagttata acaaaatctt ctgataaact atattcatca
     5461 atatgttttt gacgatcttc ttctgtgaga accacaagcc tctttgcata tttttttatc
     5521 aattttttag ccttgaatct cttcttttct ttcagcattt ccattgtcca gtaattaaaa
     5581 tgttcccaag aaatcaattt gcactttgtt cctttaattg cccttgtagt atacaaagac
     5641 aacaaagtat caatatcaat tactatatca aattcatttt tgcatatata cttgtgcaat
     5701 ttccaaaccg gatataaata agttctatat aattttccct ccgttttagg atttaacata
     5761 taatcaacct taatcgtctt atctaaccca aaataaggct ctccactatt ccaaaagctt
     5821 aaaatagtga catcataacc taaatcagac aagctattgg caataatact ccctactctt
     5881 tcagttcccc cagaacgaga aattacacca ctaatgaaac aaattttctt caaagtactt
     5941 ttctccttta taagtgtaaa aattttttca gtgcaagtaa cctgttaaat tttttttgtc
     6001 caattgttga ttttaatcta taaacaaatt tttgtttagg tgttttccaa gatgcattat
     6061 attgatgaat gcttattgta ttactagttt tataaatttt tccagtatca taatccttgg
     6121 cagcaaaata atccgtagga tagatatgca ggccatttat tatttgttcg ctgttatcgc
     6181 gaaccaatcc aatagattca agataatctg taatataata cggactcggt ttcaagttca
     6241 atgtaccatc attttgataa aaacttaatg aatcgtaaat atcccttaaa cctttaataa
     6301 cagaatgtcc ttcttctgca ccaaaaccaa taccgccatt aacccagatt tccccttcaa
     6361 atcccatata ggcatgattt tcacgaagta tatctaacga cttaattagc tcaacatctg
     6421 tatctaaata aaatccacca tgattataaa taatatcaaa tcttgcataa tctggaacaa
     6481 aaccccattt acgttcttta tatgcatcgg ccatataacg gttcttattt aaatcataat
     6541 ttgattcatt ccattcaata atctcataat cagggcaata ctttttccag ctttctatgc
     6601 actttctatc ttttttcgga agtggatttc ccccgaacca acagtaatgt atcttcttag
     6661 gaatcataat atctctcctt ttgtattttt ttcactataa taatgctctt tccttttcac
     6721 aacttcaggc atggctaaaa tcatataaat cagtggcccc gttgtttcac ccctaagtgt
     6781 tgtgttatac acaaaaaaga ataccgacat aattgcaata actttaacaa gacttgcatc
     6841 tttatccttc ataaaagatc tttttgcatt gaaaaatgtc tttattataa aaaataggaa
     6901 tatcgaaact cctaatattc caacttctat aaataaccaa atatgatgaa aatactgata
     6961 atgaagatta ccaaacgatt tataaaactc actgacaaaa aaattggtaa actctgcatt
     7021 gccaatgcct actcccatta ggactaatct aaaattatta ttaaatattt catctactac
     7081 tctagagata ccagttattc gatttaaatc accaacacca tcatatccag aatctcttga
     7141 cgcatattct aaaagacctt gaattgtgaa attatcagca tagctttgtc cattatcgtt
     7201 caatgtaata aatattgtta taaaaaagga taatactatc gacacaagta ttacgcatga
     7261 taatatcttg atcactactt taaaatcaac ttttaaagtg aatactattg caacaaatat
     7321 tataaataat tcaggaataa aaactttcaa ttcagctaat gcagacataa aaagtgctgc
     7381 acaaacacat aaaataaaat ggatgaaatt tttttctccc ttgaaatatc cgattgtaac
     7441 aacagtaact gtcacacaca aaattaaatt caatggcata ttacaacctg aataattcca
     7501 aaaaactcca cctatcatat ctcctacaat tgttccagtt ggataggtga ccatgaatct
     7561 ttcaattaaa caaagtggca gtgaaatcca gaataatttt tcaattatag cgaatacttt
     7621 atagtaatct ttaattgtta aatatgttgc acaatttata tatactatga acatacgagt
     7681 gttatttcgt atcccccaga taatcaatcc tatattttct aaattaataa ctgagctaca
     7741 aatagaaact agtaaaaaca aaactatcag catctcattt ttgctagcat atattccttt
     7801 tctgcgtcta aagctaagtg catttaataa cacaaagata ttaattatat ctcccaaata
     7861 taaaattgct cttggggcgt taaaagtata tacaagaact atggagattg caaaaaatgc
     7921 caacgtgaac aatgtccaat ctgctcctga ccatcttttt attgaaatac gcatcgttat
     7981 tcaccttctt tcgctaaaaa ttattctaaa aatacttttg ttaaattaac ataccctatt
     8041 atttttgccg gtacacctgc aagaattgca ccattctcta gacaatcttt gataacaata
     8101 gctcctgccg caacaactac attgttagct atttttacat taccaataat ctttgcaccc
     8161 acgcatacac gtacattatt tccaattaca ggacacttag gatcgattcc atcatttcca
     8221 atacagttat tcccatataa gtaacaattt tttcctatta tggcgttacc attaataaca
     8281 attccttgtg tatggtaaaa tcttacacct tcatcaaata cattttcacc acactctata
     8341 ccacattttc ttcccaaact attcattctt ctgcaagaca caatatacat aatcgcataa
     8401 atgatatttt tctttctctt tgaataataa taagatgtaa ttctcattcg acgagtatac
     8461 cgccaagcgt tgtagtccgg atgtgatttc attattttta gtaattttgt gtgttgtcct
     8521 ttagattcta tatatagatt cttttccata cgaagaaccg cttttaaata ttctttactg
     8581 ctaatttcca ttcatatctc ctattaattc aatttatact taacaatttt attcggattt
     8641 cttgcaacaa gagcatcatt caatgctact gaatctacaa cgatggatat gttatcttca
     8701 tcgttgattc caccggatac aatattactt ataaaaatcg taacattttt tccaaaagaa
     8761 gggatgtccg tgatgtataa tgcccatttt ccgatatttc cagttattta aacctaacga
     8821 atttttacaa tagtacaagt atcacaatca ttaatatata actggaatat aaatctccaa
     8881 aaattcacta tagtacagtt ttttatgatt aaaatctcct atttctaccc attccgctta
     8941 aatatgctaa tgttttaacc ccaataatgg attggtttag taaaaaatag ttaaatattc
     9001 gttgtttaat tggattttgt ttatatgggt atgagtgaat acaggcaatc aactcatcgt
     9061 tcataagtat cttagacaaa taggagttta aatcaaattc tttattaaaa ctagcatatc
     9121 taactgcctt ttgaatacaa gtacgacagc gtcccaacaa aagtctcctt gttttgttta
     9181 caaatatctc attttctaat atatagctta aatcgccttc aattttttta actaattcaa
     9241 tattacaatc gaactcatta tctctaaaat tatgtgtcaa tgaattatta ttattacatc
     9301 tataataata gatacaatct ttaataaaac ttgttatttt tgaatgttgc aaatatgaaa
     9361 tatggaatat gatatcttca gatatataat ccctttctga cggaaacttg atattgtgct
     9421 gttgaattat ctcagatgaa tacagcgcat gccaaactga cattggcaat ataaaatcga
     9481 ctttataact cggctcagtg ccaatcattc caagcaacac tttttcaatt atagcgttat
     9541 ttgtaaatat cgtactatca tatcttgctg gctttgaaat aatattatta tcatcataat
     9601 attcataata tccagcataa catgtgtctg cattactcct ttttatacca tttaacattg
     9661 cttcaatata attagctgca acaaaatcat cagaatcaat aaaagcaaca tattttcctt
     9721 ttacatgttc aagtcctgta tttcttgcca atcccaagcc agcattattt ttatgaataa
     9781 tacgaatcct tgaatccttt aatgcatagt cttcacatat tttattagat gaatccttag
     9841 atccatcatc cacaattata atatctaggt tcttatatgt ttgattacac actgattcca
     9901 aacaacttcc tacatacttt tcaacattat atacaggtat gattacacta attagatcac
     9961 tcgaattcat ccttctatcc tttcactcac gttttctcaa ttagtcaatg acggcatcaa
    10021 cgattttctt aaattctctg aaaaaaatag actctgatct tgatgatgtg aatgacatac
    10081 tatcttcttc tagaaaaatt atttccttta aattatttat taaatcaata atatgatcct
    10141 cgtaagtatg ctttcccttg gaataatgaa aatatacttt tgtattacac aatattgcaa
    10201 ttttatttct taatatgatt atttcactca ttcattactg ccacatatac acaacttata
    10261 accaccccct gcaaagttta gggtaagtta tcagataata tcttcctctt catcatccag
    10321 aatacaaaat ctatttattt atagaaatat cattaatgtt tttatataat gtaccgtgct
    10381 tgtactcttg ctctattaca agcactaaaa cactactatt catttgaatt cttacacgga
    10441 taaaaattta catcgtctat ttcaatattt tttctctcta ttttattcac ttaataagtt
    10501 cattacataa tcgaaacatt ttttattttc cttgtcatca ctccttgctc cctttttgat
    10561 acagatttaa aattctatca aagaaatttc catcgttaaa tttcgttatt gccaaatcct
    10621 tcgaacaacg tgaaaatttt acgtatttat ctttatcatt gatttttagt attgcagatg
    10681 tcaacgctga tacgtcacca ggttgaaaca aatatccatt ttctccatca cgtactgctg
    10741 aagaaatatc tcctacatct gtagcaatta caggcattcc ataagaaatc gcctctagaa
    10801 tagatatagg caatccctcg ttataggacg gtaaaatcat cacatttgat cttctatagc
    10861 actcctcctt tttttctccc acaacccaac ctgtaaattc aaaatacgat tgcaaattat
    10921 attcttctat caattctttg agcgatattt cttcatcacc tgctccagca atctcaaaaa
    10981 taaacttttt ttctggatat tgttctgcaa tgtttttaac tgcttctacc aaatcgtgta
    11041 caccttttcg ctttatcaaa actcccataa acagaaatcg gcacattttt gaattccact
    11101 ttatggtata ttctggaact acaatagaat tttctacaac aacagtttta caatttggct
    11161 caatttcttt aattatcttc ttccattttt ctccaagtac aatcgtggaa tcagactctc
    11221 ttaacagcaa gcgaatctga ttttgttttt ttacagaaac actatcatac cattttttaa
    11281 attccgatcc atgaagatgg atgattacag gaatatttaa taatctaaac cacttattca
    11341 gcacatattt cctactaaaa ctccctctgt acgacatgtg catatgaata atattaggtt
    11401 tactcaataa agagatacct ataattttag caaatgcata cataaaatat aaagatttaa
    11461 atattgcatt tccaccctta taactcggta taaaagtcat ctcgatattc tttttttccc
    11521 aattaaaatt gagcatttgt gtaattatac ttgtaattcc accttttacc gagctgtcat
    11581 taccaatcat aagtactctt tgcataacgc tttactcctt atatttatat tttttctatt
    11641 aatacctcta tcgaattaat aatcgtttcc gtgttagata catacttgtt aaacatccga
    11701 actcttgttt ctttaacggc atcttctaaa ttttctaatt cataacaagg atagataata
    11761 ttcatctcat caaattgtct aattaactgc agttggtgat catcgacatg ctcaccatac
    11821 ttagccaatc gaggaacagc aatgaccctt ttaccttttt ttacggcccc aattatagca
    11881 cctgtacctc catgagtaat caccatttct gctttttgca tagtttcagt aaactcatcc
    11941 ctatttagaa atgctttata ggaataattt agtggtttat aatcactata accactctgt
    12001 gcaaatgtac tttcgattat tactcctttt tcaattaaat catcaatagc ttttaacaat
    12061 ctatcaaatt gaaatttttg tgaacctaat gttataaata tcattttttt ctcctattat
    12121 ttaatatatc ccacccaaat aaatagcatt aggataaaat tgtagcatag tttcccactg
    12181 aacataaaat tgatctgcaa aatgatacat caattttcct gtctgagtag ggctggttac
    12241 ttttgcaaac gattcaatat aaatcagttt ttttcccatc atttttgtta ttaagcagat
    12301 agggatcata gctaaaacac ctgtacaaat cacgagatcc ggtttttcct ttatatatat
    12361 aataattgat ttgaataaat taataagtat agtacacaaa aaaaactttt cttttctatt
    12421 tacttgtctc atgtagtacg ttttttcgcc ttttgactca gctgaatatt ctgtctgttc
    12481 tgttataaca aaactctcat acttctccat cagtggtttt aacatcatta actgttcata
    12541 atgcccccca gaggaagccg caaaacatat ctttttgttc atattaacct ctctatcagt
    12601 cacgctgaaa aatatctcta gtgtaaactt tttctttaat atcatccaag caggtatcat
    12661 atcgattggc aataatagaa tcacacatcg ccttaaactg ctctaaatcg ttgataacct
    12721 tacttccgaa aaaagtctct ccatctttca gtgcaggctc ataaatcacc acagtcgcac
    12781 ctttagcttt tacgcgcttc ataactccct ggattgaact ctgacgaaaa ttatcactgt
    12841 tgctcttcat ggtcagccgg tatataccga ttaaaactgg attttccctg ttgacatcat
    12901 actgattata ttcagcgtag ttgtaatagc ccgccatctg aagaacacga tctgcgatga
    12961 aatccttacg ggtacgatta gattcaacga tagcctcaat caaatttcca ggaacatctg
    13021 catagtttgc caacaactgc ttggtatctt ttggcaagca ataacctcca tatccaaagg
    13081 atggattgtt gtagtgagtt ccaatacgag gatcgagaca aacaccattg attatctgct
    13141 gagtattcag ccccttcatt tcagcataag tgtctaattc attgaaatag ctgactcgca
    13201 gtgctaaata ggtatttgca aacagcttta ctgcttcggc ttcagtgaat cccataaaaa
    13261 gagtatccac gttctcctta attgcacttt tctgcagaag tgttgcaaaa acatgagcag
    13321 catttaacaa gcgctcatca gtcatatcag taccaacaat aatacgagat ggatacagat
    13381 tatcatataa agccttgctc tcgcgcagaa actctggact aaaaataatg ttcttactcc
    13441 ccgttgtctc acggactgat tcagtgtagc caacaggaat tgtagacttg atgaccatga
    13501 tggtatcagg attcacgtcc atcacaacct caataactga ctcaacagca gaagtatcga
    13561 aatgctgagt acgactgtca tagttagtcg gtgtagcaat aacaacgaaa tctgcggatt
    13621 tataagcgct ccttgcatcc aaagtagcag ttaagtcaag ttctttttct gtaagatact
    13681 tttcaatata ttcatcctga attggagact tccgtttatt aatcagttca atcttttcag
    13741 ggataatgtc taccgcggtt acctggtgat gctgcgccaa tagtgtggcg atactcaaac
    13801 caacataacc ggtgccggct actgcgatgt tgtacttttt cttcacaata tctcctcttt
    13861 cctctcttaa gtatggagaa tgttactcct acggctattc atcatgtcaa aataacacag
    13921 cttagaatgt tgataaaata attttccaga atgtgataaa atctctttca tttataaacc
    13981 acacatctaa ttttatctat tctccacaag acatgtagag tattctttaa taacaagata
    14041 cgaccttact aaatacttca aaaaattatc atatttaatt cataaatata aatcttcatt
    14101 ttaaatgtat gcccaaaaat ttatctgtaa tgcgtcatca aaatctagat tccaaatctt
    14161 tatcaatttt tatccactca ttctaccact ttagtaatat tttctattta taacactttc
    14221 gtcctacatt gatccccccc tctgaagtac agtttttact gtcttaaata aaattttaaa
    14281 atctaacccc atactccaat tttttatata ttccgtgtca agttttgcta cttcttcaaa
    14341 atcagtaata tcacttctac cacttacctg ccacattcca gtaattcctg gcttgatagc
    14401 aattcttgca cgatgatgat aatcatattc ttcatactct gatttaaggc atggtcgaaa
    14461 tcctataagg ctcatctttc ctgttagaac gttgaaaaat tgagggaatt catctaaaga
    14521 cgtggatcta ataaattgac cgattcctgt cttttttgtt ccatctggaa gtattttatt
    14581 tccgataatt cttggatcaa actccatctt aaacataaga ttattgctca tcttattttc
    14641 tttcattaat tctacttttc tttcttctgc atccatatac atacttctaa acttatacat
    14701 cttgaacggt ttaccattct gacctatacg ttcttgtgca aagaaaattg ggcctggtga
    14761 attaatataa ataagtggag caataaagat aaaaataatt cccgttgcaa tacatccaac
    14821 aatacctgca agaatatcaa ttgttcgttt tataaatgcc tgttgagttg tcataacatt
    14881 gatacttgtt gtaagtactg tatattctcc tatacgacca atagattgtt ttcccaaagc
    14941 actatctgat acttttgcaa gattcaaatg tacagtaagg cccatttcag agcatcgttc
    15001 aataaattct ttcatacacg aagtactttt attcaaaata ataaagactt catctaccca
    15061 tccctgacat aagtaatccg ctgcattttt ggaatcagaa acaactgtca ctccctcaac
    15121 atcttcacca atcatgcttt tatctatgat aatgacacca tttattttat acattttata
    15181 gttatgctct ttcacattac gaataactac ttcagcaata ctactagatg taactataat
    15241 aagtgatttg ttcccctcat ttttcatttt atgtttcaag taatgtttcc ataagaggcg
    15301 agttatatat gtaattacaa cgtataaacc tcccatcaag aaaagaacga ctctagaata
    15361 atcatttcca ccctgaacac taatgagata caaacttcct aacagcacca acataatcgc
    15421 ttgctttaca gaagaagcaa attctatata gtaccctctt tttaatacac ctctatatgc
    15481 ttcaaacatg aaaataacta aaagatctgc cagttcaatg aagactgcca tgttttgata
    15541 cattggaatc acataaggat tccaatctac ctggcgtaag acatacgata gataaaaagc
    15601 aagctggata cacaataaat ctaaaataat aaaatcaata tgtttaaccc atccccttga
    15661 acttttcttg tacattcctt tttactcccc tctttttaat aaaataaatt gtattacttg
    15721 ttttctctcc taaatatctt tctgaaggat tattgactcg acaaattggc ttgtggatat
    15781 tcttatatat agaacatccg taagccaact taaaactcct taacaaaatt tttttactca
    15841 tattgtagat caaaacaagt atccaagaaa tcatatttta tttctttttt ctgcttactc
    15901 aacaacagct aaaagaaaaa tcatcataaa caaattcaac taagccaacg atccccttgt
    15961 gaaatcaagt tttttcttga tttacgaata aagagctaaa ataaaccaaa cttcttcttt
    16021 tttacctctt catagactgg ataactgatt tcatccccgt tgatcagatc ctttgcgact
    16081 tgtttcatct gtactacttt ttccttacca aagtcctttg caatctgctc atagcactct
    16141 tttaagtaaa aggtgcgctt agagacccca tgggcatcag aagcagccat ctgcacgaga
    16201 ttatgtttga ccatcgtttt ggctgttttc tggatgtctt tgccaaatac cccaacataa
    16261 cttggcgcag tgagttgggc caaacatccc atatctagga acggaagcaa atgatttggg
    16321 tcttcgcgaa aatgcacgtt gcgttctgga tggacgatta ctggtgtctt tcctagttct
    16381 aacaactcaa aaaaaagccg ttctgcatat aacggcactt ccagtgtcgg aaattcgatc
    16441 agaagatatg tatcatctaa atctgtaaat aatatttcat ctcggtggat gtcttcgatg
    16501 agcgttccgg taatacgtac ttcttgtcct tccaataacg tcaaaggcaa ctgtcttttc
    16561 tctaattcgg cttgtaacga agcgactaga gaaatgactt gtgatttttc attgctgtat
    16621 tttccattat tgtggtgtgg tgtgcataaa atatgcgtga tgccttgctg aatcgctttt
    16681 tcagccattg caatacttgc ttctaagctt tctgccccgt catcgattcc tggtaggata
    16741 tggcaatgta gatcgatcat ttctcttgtc tcctctcttc ttattcctta atttccgtag
    16801 taataaccag catctttgct atgttctgct ccgttgtaca caacacctaa gactcgtgct
    16861 tgtaccatct ccagcagttc ttttgcttta gtcaaggatt ctttacgtga catattttca
    16921 cgtacgacca ataaagtgcc atccgttttt aaagacatga tctgtgcatc tgttacggct
    16981 actactggtg gcatatcgaa tatcaccacg tcaaataact gtcttgcttc ggataggatc
    17041 tgatccattc ttggcgagct taacaattct gatgggttgg gtggcttagg tccactaggc
    17101 aaaatcgaca agttctcgac tggcgttcgt tggatcacat ctgctacact tcctgatgaa
    17161 ctaagtgctg tacttaatcc actggcattg ttcagctgga atgtcttata gacgacgggt
    17221 ttacgtaaat ctgcatcgac tagtagaact cgttgacctg atttagcaaa aacgactgca
    17281 atgtttgctg cagtggtgga tttcccttct ccaggacctg atgaagtcac aacaatcgtt
    17341 ttgatttgct gaccagctgc ggaggcaaac tggatattcg tccgaatcgt acgatattgt
    17401 tcagcgattg gtgaagaggg attggtgagt gtgaccaaac tgactgcatg agtttgggct
    17461 gctttttgtt tctgtgttcg tgccattttc tttcctccta gactcttgag cggcttctgc
    17521 gtgatggcgc tgttttatcg tttgtagctt ttggttgtgt ctgagctggt ccttttttac
    17581 ggataccagc attcaattct ttcgctgaca tttctggaac tgttcctaag attgggaatc
    17641 ctaatgtatc tgtcacatat ttttcatctt tcactgtccg atcgagtaat tccaataaga
    17701 atgccagacc tacacctacc atcattccta acattaagcc agtcattaaa ttcaatttat
    17761 tatttggcga aacaggtgtc gtattggcaa ctgcacctga aatgatcgag attttatcaa
    17821 cattcatcac atcttttgcg ttctcttgga aaacttgtga agttgtgtta gcgatctgtt
    17881 gagcagccac tgcattcgta cttattgcct gaatcgagaa catttgtgaa ttttctgcct
    17941 gattaactga gatggcatct ttgatttgtt cagcagtcat tttcacattg tcttctgttt
    18001 ctaaacgatc tttgacttca ttgagcacta aatcactgac gatcatatct ttatacgtat
    18061 tgatcatttg caggttcgta ttgacatcat tgacatttgc agtgtctgat tgtggcagtg
    18121 tcacgatcaa ttgtgcttgt gaactgtatt ttggtgtcaa gatgaaaaat gttacgattc
    18181 ctgagatggc caagccagca aacattgaga tgatgattat taccaagtgt ttgcgtaatg
    18241 tttcaaaaat ttctttcaag ctgattgtct cttccatttt ttctccactt tcctatttat
    18301 tctctagatt catttgatct tttaattctt ttttcacacg atcaagttct tgatcactga
    18361 ctttttgata ggagatatta tcttgcatga agccttctcc ttgcagctga tcttgcttaa
    18421 tcgtaccaaa tgcctcccga taatctaaag caattttttt catttcatca aagttcatgt
    18481 ctgttttcat attcgactct agagcattca aaacggattg atagttgctg acaccgttta
    18541 gactcaatac ttttttcgct acgccttcta cgatctttct ttggcgccct tgtctaccgt
    18601 aatcaccatt tgggtcttca taacgcatac gagaataaga taatgcctga tccccgttca
    18661 atgtaatttt accgattgga aagtcatagc catcttgtga gaaagtgagg tcattattga
    18721 cttcgatacc accaactgca tcaacgagtt cctttagccc tttcatattg atagagacat
    18781 agtgatcgat tgggatgtct acatattttt gtacggtatc catcgccatg gcagctccgc
    18841 cgaatgcgta agcgtggttg atcttgtctt ttgtgtcacg acctacgatg tcgacatacg
    18901 tatcacgagg aatgctgacg atcgttgtct gtttgtcttc aggactgact gtggcaacca
    18961 tcatcgtatc tgatcttcct tgatcgaccc gacctaaatc tccagtatca atccccatca
    19021 atagaacaga gaaagattct tgtttgtcca gatcaactgg tttcgttcgt tctccttggg
    19081 ttcgttccac tgattcgtaa gttttctcaa tagaatgtga gagatccaga tataattttg
    19141 ctcctactcc tacaattgct aatactaata cgataagaac tgctaaaata atcagaaata
    19201 tttttttccc tcttgatttt cttcttcgtc ttgccatagt ttcctccgtt tgctaacctt
    19261 gcttgaatca acgttttaac tgtcaatagc ttatattgct atagcccatt tgctcagcaa
    19321 cagtttgtgc ttttgttgcg tatgctgcgc ttattttttc attccggatc tgctctattt
    19381 gactgagcac cgtcaaatat tgatcgtaag tagcggtggc agtcacttga ccatccttta
    19441 acatcgtatc gaataactga tctagttgcg tgacttgcgc gatctgaata tcagcgaatc
    19501 ctagatattg cataacaacc gttttccaag gactatcttt aaaaaagctc atattttcac
    19561 gtacacgagc gacatctgca cttgtcacct tttctttgat gatgacatcg tctacagctt
    19621 gctgccagtt tgatacattt ttagtaaata aatcgttgac agctgtctgg atcttcagtt
    19681 ttgattctgc actatctaac tgtgtcacga ttgctttctt ctcatcttgg attgcaacag
    19741 cttctttagg caattctgat tctttgatgt cgtaatcatc tgctgaaagt ttgacagagg
    19801 atagcttatt tgacaaactt gtgattgttt cttctgttat ttcagggttt agaaaaattt
    19861 tttcttttgt actatacagc ttgtctactt cttttgataa agaagtcaag gtgtcttttt
    19921 gattggtgat ggcagattct gcataattga tccgttgctg atgttgccgc tgattattga
    19981 tatggtatcc aaacaaacag atcgcaatag cacctgcacc taccgccaca gcagtcaatg
    20041 acttgacgaa tggcttttcc atagattcac tcctaagtat tgatgataga taaattaaga
    20101 tataattcac ttacataaaa atgacagctt tttatttaaa aaaacataat taaactaata
    20161 tattcacaga aatatttgta aacaagtttt ccctatataa tcataccaca aactgttttc
    20221 ttgactaggg caatttacga tttaaaagta aataaaacat taataagata aaaattaatc
    20281 gatttcattt ttatttccat aattttcata ggtgttttta aaagttacac aaaaaataga
    20341 ataacaaaaa aattaacatt gaattaataa tagttatacc cgtggtgcta gttaattttt
    20401 tcctagagga aaatgtctaa acatgttggt aaatcaacaa aaaaacgcat atagtagtct
    20461 caaaactccc aagaaatgag gactacatat gcgttcatca aaatatactg aacattatac
    20521 agatactttt ataacattca aggaaattat gagaactgtt tctaacatat accacaattg
    20581 tgtaccagat aagattaaaa atcgaagaaa tactgatcaa ctgaagcaac gcgatacagt
    20641 gattattgct tgtgttatat ggggcataat taatggctat actagccaaa gagccacgta
    20701 tagagcggtt tgttccgtct tatttcctaa tggtgacttt cctagtagga gtcgattcac
    20761 tcgcttaagt tcaaacttag cttacactat caagattatt cgatactttt ttatcaagaa
//
LOCUS       DAFEOM010000042        20782 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.043, whole genome shotgun sequence.
ACCESSION   DAFEOM010000042
VERSION     DAFEOM010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.043"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(18..1574)
                     /gene="rny"
                     /locus_tag="LP524_001773"
     CDS             complement(18..1574)
                     /gene="rny"
                     /locus_tag="LP524_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBL2234811.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(1985..3034)
                     /gene="recA"
                     /locus_tag="LP524_001774"
     CDS             complement(1985..3034)
                     /gene="recA"
                     /locus_tag="LP524_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBL2234812.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(3143..4384)
                     /locus_tag="LP524_001775"
     CDS             complement(3143..4384)
                     /locus_tag="LP524_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBL2234813.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(4624..5202)
                     /gene="pgsA"
                     /locus_tag="LP524_001776"
     CDS             complement(4624..5202)
                     /gene="pgsA"
                     /locus_tag="LP524_001776"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBL2234814.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(5411..6334)
                     /locus_tag="LP524_001777"
     CDS             complement(5411..6334)
                     /locus_tag="LP524_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2234815.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(7073..8359)
                     /locus_tag="LP524_001778"
     CDS             complement(7073..8359)
                     /locus_tag="LP524_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBL2234816.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(8352..9620)
                     /locus_tag="LP524_001779"
     CDS             complement(8352..9620)
                     /locus_tag="LP524_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBL2234817.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            9872..10333
                     /gene="mscL"
                     /locus_tag="LP524_001780"
     CDS             9872..10333
                     /gene="mscL"
                     /locus_tag="LP524_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HBL2234818.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(10392..10838)
                     /locus_tag="LP524_001781"
     CDS             complement(10392..10838)
                     /locus_tag="LP524_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2234819.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(10931..11176)
                     /locus_tag="LP524_001782"
     CDS             complement(10931..11176)
                     /locus_tag="LP524_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBL2234820.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(11290..11868)
                     /locus_tag="LP524_001783"
     CDS             complement(11290..11868)
                     /locus_tag="LP524_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL2234821.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(11945..12475)
                     /locus_tag="LP524_001784"
     CDS             complement(11945..12475)
                     /locus_tag="LP524_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBL2234822.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(12479..13339)
                     /gene="rlmB"
                     /locus_tag="LP524_001785"
     CDS             complement(12479..13339)
                     /gene="rlmB"
                     /locus_tag="LP524_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBL2234823.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(13332..13727)
                     /locus_tag="LP524_001786"
     CDS             complement(13332..13727)
                     /locus_tag="LP524_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBL2234824.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(13724..15136)
                     /locus_tag="LP524_001787"
     CDS             complement(13724..15136)
                     /locus_tag="LP524_001787"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBL2234825.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(15133..15669)
                     /gene="cysE"
                     /locus_tag="LP524_001788"
     CDS             complement(15133..15669)
                     /gene="cysE"
                     /locus_tag="LP524_001788"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBL2234826.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(15755..15994)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(15991..17451)
                     /locus_tag="LP524_001789"
     CDS             complement(15991..17451)
                     /locus_tag="LP524_001789"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBL2234827.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(17621..18778)
                     /locus_tag="LP524_001790"
     CDS             complement(17621..18778)
                     /locus_tag="LP524_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBL2234828.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(18812..20182)
                     /gene="radA"
                     /locus_tag="LP524_001791"
     CDS             complement(18812..20182)
                     /gene="radA"
                     /locus_tag="LP524_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBL2234829.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(20245..20772)
                     /locus_tag="LP524_001792"
     CDS             complement(20245..20772)
                     /locus_tag="LP524_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBL2234830.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         5473 a         4592 c         3706 g         7011 t
ORIGIN      
        1 ttcttttgta ttttttatta tttagcataa tcagttgcac gagtttcccg aataacgatt
       61 actttgatgt gtccaggata atccagttct tcttcgattc gtttacgaat atcacgtact
      121 aaacgaacag cgtctaaatc cgagatttct tctggcttga ccattacccg aacttcgcgt
      181 ccagcttgaa ctgcgtaact tgattctaca ccggcgaagc tgttcgaaat attttccaga
      241 cttcttaaac gacggatgta gttttctaat gattcacttc gcgcaccagg tcttgctgct
      301 gagagcgcat ctgctgcagc tacaaggaca gagataacag aagtagcttc cacatctcca
      361 tgatgagaag cgatcgcgtt gattactact gggttttctt tgtacttggc agctaattcc
      421 gcaccgatct caacgtgaga tccttcaatc tcatggtcaa gtgctttccc aatatcatgg
      481 agcaacccgg cacgttttgc taattggata tcttcgccaa gctctgctgc taagatacca
      541 gctaattttg caacctcgac cgaatgcttc aagacgtttt gtccataact agttctgaat
      601 cgcagacgtc cgatgatctt gattagatct ggatgaagtg tatgagcacc gacttcaaac
      661 gctgcctctt ccccgtattc gcgaatacgt tcatccattt ctttacgaga tttctcgacc
      721 atttcttcga tacgcgctgg atggattcgt ccatcttgta tcagtttttc caaggtcatc
      781 cgtgcgattt ctcttcggat tggatcgaat ccggaaagaa caactgcttc cggtgtatca
      841 tcgatgatta aatcgatacc agtcagcgtc tcaagcgttc gaatattacg accttctcgt
      901 ccaatgatac gacctttcat ttcatcattc ggaagggtaa cgactgaaac agtcgtttct
      961 gaaacttgat ctgctgcgca gcgttgaatc gctaatgata ataagttttt cgctttgcga
     1021 tctgcttctt ctttggcacg ctgttcagat tcttttacca ttagagttaa ctcatgattc
     1081 agctcttctt cagtcgattt catgatgaca tctcgtgcgt cctcttttga taaaccagca
     1141 attttttcta gttctttttg ttgatcttca atcatttttt caacgtcttt ttctcgctct
     1201 tcaattaatt gctgttttga actaagtgct tcttctttac tttcaagtga gttctcgcgt
     1261 ttttctaacg agtcatcttt acgattcagc gtttgttcgc gttgaatcaa acgattctct
     1321 tgagatttta attctaactt actttctctc agctcacttt caacttcaga acggtatttc
     1381 tgattttctt ccttagcttc taacaatgct tcttttttta gagtttctgc ttcttttttt
     1441 gcactatcga tgattccagc agctgatgat tgtgcaccag ctatttcttt ctcgtgacgt
     1501 gactttgcga ccataaaccc taaaccaaga ccgacaatta aaccgatgat agcgaggaga
     1561 atgttgaata ccataaaatc cacctccata ctatcttttt catttttatc atctatacag
     1621 cttctatgat aaattattct aaactcgtca atatgcatct tcgcatatat gtgtacaaaa
     1681 aacatgcaca actttattct aatgtttgta ccccgtagtg tcaactttaa ataaacaaag
     1741 aaacggcttt ctacttattc attaaacgat ggtttttaaa ataaatgaca ctagtttcat
     1801 ataggtaaac ctttaaattg agaaactttt ctattaaatt attctttgta gatgaacaga
     1861 tatttttatt ttatgtaccc actcgtataa ttaaataact cacgatttct ttattcaata
     1921 aaaaaacggc aggtcaagga gagtacctac tcttcagacc agccgttttt gtttatcata
     1981 atttttattc ttctactaaa ggcaattctt cttgtccttt ttcatcttct gggacagcga
     2041 cttcttcacc aataccataa gctgctctaa ctagagcaga aacttctgcc atcatttcag
     2101 gatgattagc catatagatt ttagcgtttt cccgtccttg accaatccga tcttctttat
     2161 aagaatacca agcaccgctt ttatcaacaa tgtctttttc tactgccatg tctagaagtt
     2221 ctccttcttg tgagatccct aaaccataca tgacatctac ttcagcgatt ttgaaaggag
     2281 gtgctacttt gttttttacg actttgatct ttgtacggtt accaacaata tctgtacctt
     2341 gtttcaattg ttctgcacga cgtacttcca aacggatcgt tgcgtagaat ttcaatgcgc
     2401 gtcctccagg agtgatttct ggattcccaa acatcacgcc tactttttca cggatttggt
     2461 tgatgaaaat agcgattgtt tttgttttgt tgatcgaacc agagagttta cgcaatgctt
     2521 gagacatcaa acgtgcttgt aacccgacat gtgagtcacc catttcgccg tcgatttctg
     2581 ctcgtggaac taaagcagca actgaatcga ctactacgat atctacagcc ccacttgata
     2641 ctaaagcatc agcgatctct agaccttgtt ctcctgtgtc aggctgtgaa agaagtagtt
     2701 catcgatatt cacacctaat ttttgtgcat attgcggatc taacgcatgc tcagcatcaa
     2761 tgaaagcggc cgttccgcca tttttttgta cttctgcaat agcgtgtagt gcaactgttg
     2821 ttttacctga actttcaggt ccatatactt cgatgatacg tccacggggg taaccgccga
     2881 cacctaaagc gacatctaac gcaagagagc cacttggaat tgtagagatt tgttggtcga
     2941 ttttttcgcc caatttcata atagagcctt taccatagct cttttcaatt tttttcagtg
     3001 cagcatctaa ggctgcttta cgatcatctg ccaatacgat aatcctcctt atatttacaa
     3061 atcagagcaa catttcatta tccttatcat atctttttta gtgaaaaaaa gcaagaaaaa
     3121 accgaacaaa tattcgcatt tatcattttc ttaaaagctg ttttctcaaa agatcaaatc
     3181 cttgcataac cgcattatat ctgatagcag cacgatctct ggcaaagtga tatttttccg
     3241 aaaccacgcc gtctttagat gaaatagcga tccatactgt tcctgcagat tgaccttcta
     3301 acgtgtccgg tccagccact ccggtgaaag ctacactgta atctgttctt gctatctcac
     3361 gtgcttgaat ggccatctgc tccacacatt ctttgctgac tgttccatat tctgctaata
     3421 gctccggatc aatctccaga aaaccagctt ttgtttggag cgagtaggta acgaatccgc
     3481 ctggaaatac ttcagataca ccagcaatgt cgcctaatgc ggcttggaag gcgccggctg
     3541 tcaaactttc cgctgctgtt acagtctgct tgttttcttt cagtagttcc actacgactt
     3601 ttgctagcga attatcatcg ccgtatccat aaaaatattc ccctactcgt tcttggattt
     3661 tttcttctaa tgctaaaaga gcttgattcc ctgcctgaac atcatttgtt ttcactgtca
     3721 gtcgtaatgt cacttcattt ggtttggcat atggtgcgat cgttggattg atctgtgtct
     3781 caatcagatc ttttagctca gtgaccagtc gcgattcacc aatcccataa aagcgcaaaa
     3841 ctttagaaat tagtttttct tcagaaggaa aacgttcctc tagtaaagga cgtacttgtt
     3901 ccacaaacat tggtttcaac tcattaggcg gaccgggaag aaggatatac gcatgtgtat
     3961 ctgtttgata aaaagtccct aaagcaagcc ctgtacgatt tggtagagga accccgcctt
     4021 cgataatctg ggactgttgc agattgtttt tggtcatttt tcgatgggtt gtttcaaaat
     4081 atgccaataa ttttttgtat ccttccgtat tttgtatgag agacttaccg aggtgagccg
     4141 cagttgcttc ttttgtcagg tcatcttctg tcggtcctaa tccgccacat aagagtatga
     4201 gttcacttct tgtttcagct agagcaatca actcttctaa acgctgctga ttatctccta
     4261 caactgtttg ataataaact tcgataccta gatcagctag ctgttctgac aagaaagtgg
     4321 cattcgtatt cacgacttgt cctagtaaaa gttctgttcc gacagctatt atttctgctt
     4381 tcattacttc ccgctccctt atatagtata cgctgtattt ttatattttt ttatttaaaa
     4441 agagggcaag gagctgtgac aaaaatcttg tcacagctcc ttgttccaaa taaacagtgt
     4501 tctcctgcca aaacctcggt cataagtcac aatatacata aaacatgaaa agctgttttt
     4561 tgaaattatc ccttgtgact cagagttaga cggcaggctc acaacctctc cactaaacga
     4621 tacttacatt gatcctttga acacaccgct gtttttgatg aagtaatcca cacctgaata
     4681 gatagtaaag atcagacaag cataaagcat gatttgatct actggaatac cgatcaggct
     4741 gaatgggata ttattgataa acagcaagat aatagcaacc atttgtgtag ccgtcttgac
     4801 ttttcccggc caagctgcag ccatgacttc tccatgttcg acaagtagta aacgcaagcc
     4861 tgtgacggct aattcacgac agacaatgat cgctactacc caaccagggg cctttccttg
     4921 gtcaaccaac atgataaaag cagtcataac tagcatttta tctgccaacg ggtccgcaaa
     4981 cttgccaaaa ttagtgacaa ggcctcgcgc acgggcaatt tttccatcta accagtctgt
     5041 gatactagct aaagcaaaaa taactgcacc tactagctgt gtgacagcca gtgtggtgtc
     5101 tcctacgtta aattgtcccc aatctaaagg taaacctaca accaaaatga aaatcgggat
     5161 catacaaatt ctaagtacag ttaatttatt tggtaaattc acactatcga ctcctttctt
     5221 ccctattcta cgtgactatc agcgaagttt ccaccgatat tatttacttt tccaaatgaa
     5281 tttattcaaa ggagtatttt tattgaaatt gatctttttg aattaacgaa gaaggaccgc
     5341 gacacagatt tgtcacgatc cttctttttt atacctacaa gtaaacagat gcgtaaacta
     5401 atttatacga tcattctgcg tattgaagag taaggttcac attttttgga ctctgattgt
     5461 tttcccctgg attaactggt acggattctc cattcgcttt gatcgttgca ttgcttgctg
     5521 caccaatcgt gatggtcgca tttgttgctg tttccggtaa cacggtactc tgtgtttcat
     5581 ttgctgccag tgtcccttga taaacatagg cattgtctac tagcacacct aaccatacac
     5641 gattgacagc agtaaattca agggttacag gcttggtagc gtctgtgata tttactgcga
     5701 tggcactgcc agaatcttct cctgtagcta ttgccatttt cttttcttca cttgattttg
     5761 ttgttgattc tactgtagaa gactctttgg tctgcgtgct ttgctctgag gaagaggact
     5821 cctcgactga tccttctact ttaagcgaag aagctgtgtc tccgattatt ggatcagctt
     5881 gacgatcttg ccaagtcata tagcccacga caacgacaat cgccaaagca accaatccta
     5941 ataaaatgac aggaagactt gtccaaaagc gacttggatg cgattcttcc tgatgcattg
     6001 cttttctaga agtacttact gtttcaggct gcggacgttc agggaccgat cctgtcaatt
     6061 tgcttttccc atcaaaaaca tcgactaaat ggtctccatc ttctcctaca gcttcagcgt
     6121 attgccgaat aaatgcccgc acgtaaaatt taccaggcaa ttggtcaaaa tcatcattgt
     6181 cgattgcttc caaataacgt ttttgaattt ttgtaatctg ctgtaattca tctaaagata
     6241 tttttttatt caggcgtgct tgccgtaatt tttctccgat ggtttcaaaa gccactcgtt
     6301 catcttctcc agtctgtttg attctccgaa gcatctgctg ttgcaacgaa agacgttatg
     6361 tttgggtgct gtgacaaaag tcatgtcaca gctccttttt tccgaataaa cattgttctc
     6421 ctgccaactc ctcggtcaca agtcacaata tccaataaat ataagaagct gttttttgaa
     6481 cttgttcctt gtgactcgga gttaaacggc aggctcacga cctctttctt aaacggtgtt
     6541 caatcagctg ttcctcggta ttagctcaaa aaccggcaaa atataaggag atatttttcc
     6601 gtatgtttct ctaatactca ggtcaaaacg ctgatttcac aacctcttcc ttaaacggtg
     6661 ttcaaaagct tgcatctgcg gtaataagtc caaccaagtt aaaaacatta agaagctgtt
     6721 tttacttggc tgttcttatt acttgatgca gggcagcttt ttcacaacct cttggtacac
     6781 ggtgttccag aagtagcttc ttcggcaata agccgaaata tcacaaaaat ttgagaagca
     6841 attttcgtaa attccttctt atttcttgaa gctgaccact tctgtcacaa cctctttgat
     6901 tacttcgttt gattttattt taatataagt tgattaaaag agagcatgat ttgttttatt
     6961 ttctcactaa tttttccaac acaactaaga ataccataaa agctgatttt ggaggaggtt
     7021 cctactacaa gtctaaaacg aaatttaaga cttttcctca taaaatactg gtttattttg
     7081 gtctcatgta aaactcgctg aaggcctctt cacttataaa tgcttcccca gctgccagta
     7141 cgtctttcat ttggatcgaa tcgatgattt caggaagatc aaataaagtt cgatcgccaa
     7201 ataaactttg cgtaaattga ttggcaatat attcgatcga atttaatgat tggaaatatt
     7261 gtccaagcat tttcttcttc agcaaatcga gatttgtttc tgacacttcc ggatcatcag
     7321 cgaatccaag aatgatttct ttgacttttt ctgctgcttt ctctggttcg tccgtatctc
     7381 cgctgaagtc cgcaaagtga aattcacgat ccaagctgaa ttcaaaacca aagctgtcat
     7441 cgataattcc ttgattgtac atcgctagat agtttcgaga agtattgcct aaaatcattt
     7501 ggaacaacag attgattgct gttttatagc gtaacagttc tgttccttct tgcggcaatg
     7561 tgtctaatcc tttaatacca agtacaaatt tatctcgtgt aatggctgct tccaatgcac
     7621 tttgtttgat gatttctttt gtgttttcag ggaaataacg tacaatttct tgctttggag
     7681 ggaaattttt tgcttcttgg ttttcccgaa taagtttcat caatttctca ggctccatct
     7741 taccaactac aaataaaacc atattgcttg gctgataaaa cgtccgatag cacgtataca
     7801 agtcttgagc tgtgattttg tcgatacttt caaccgtgcc cgcaatatcg atatgcaatg
     7861 ggtgtgtagg atacaagtta tttagtatgc caaaaaacat ccgccagtta gggtcgtctt
     7921 catacatttg gatttcctga ccgatgattc ctttttcctt gtttacagtt tcttctgtga
     7981 agtaaggtgc ttgtacaaaa tcgatcagag tcgtcagatt cttttctacc tgatccgtcg
     8041 tagaaaacag atagctggtt ttcgtaaaac ttgtgaaagc attggcggaa gctccttgtt
     8101 tcccaaataa ttgaaagaca tcaccatctt ctttttcaaa caacttatgt tctaaaaaat
     8161 gggcaattcc atctggaact ttcacctttt ctttttcacc atagggaata aattcgttat
     8221 cgatcgaacc ataattcgta ctgaacaaac catatgtctt gtgatagtca tttttaggca
     8281 acaaatacac ggtcaaaccg tttggcagta cttcatgata aagtgtttca ttgatttgct
     8341 catattctgt tttattcatt tgcatttcct cctgctaaaa agaagattgc ctgcaattcg
     8401 atttgctcag ctacttgttg tatctctttg atcgttacgc tctccatacg tttcaaccat
     8461 tcttcatcag ataattttgt ttccggcagc cagctgtcca aaaaagaagc ttcgattgcc
     8521 gcttgcggag agtctagaga aagcagatat tggtttctca gcattgcttt tgtctgagac
     8581 agttccaact ctgttatttc accatttcgc aaactttcta attgctcgtg aatcagatga
     8641 agtacttgat ttcggttctt ttcatcgata ccggtttgga cactcatgaa tcctcggaac
     8701 gtatccacgc tactagaggc ataataagct agactttctt tctctctgac attcatgaaa
     8761 agtttagagt gaggaaaacc gccaaataaa ccgttaaaga ccattagcgc aaaacgttct
     8821 ggttcatcgt aatacacatt tgtctgataa gctaggttca gctttgcttg aacgattggc
     8881 tcacgtactt gtcgttcctt cacgatattc acttgaggtt gggtataaaa cagatctgga
     8941 taaactgttt cccgcgactc aaatggtaat tgcccaactg cttcagctgc tttgtccgga
     9001 tcaacatcgc cgacaacaaa aatgtcgatt tgatcatttg ccatcatttc ttgatagtag
     9061 gacgctagcg aactagcagt gatcttgtcc aaatcttcga ctacgcccaa ccctgggatc
     9121 ttttgttccg ggctgttttc aaaataaagt tcttgcaagg cataggaagc aaaagtttgt
     9181 ttatcttcct tcaaactttc caaatataga cgcagattgt tcttttccaa gtcaaacgtc
     9241 aactgatcaa actgatgatt tttaatattt ggataaaata aaacctcttt taaaaaatct
     9301 accgcttgag aaaaaaggtt tggatcattg acatattcac cattcacaaa actgatccct
     9361 gcgtttagcc aatgtaaatt tccttttttt ctaactgata atccaaagct tgcgccataa
     9421 agctctgcaa gttgcgcact taactttgtc tgatccggat aattcaaact atttgtttcc
     9481 aacatgctgc tgagaagtga acgtttcgtg atgacttctt gttgcagacg cgcagtaaaa
     9541 cggataaaaa tccggatcgt tttgtattgt tttgttggta agacatgaag attcacgcct
     9601 tctgccaatt gatacaccat atttatatgc tccttttcac agttgattgc ccgcttactc
     9661 ttttaatggg tcaacctgcc ttttttactt attcctctgc ttgaaattta taagttcagg
     9721 caattcgccg ttttttctaa atgatgttac actttatagt atagcataga tgttttttat
     9781 caaaagaaac agacggctaa aagcgtatat tgttttttta ttttttattt ttcgatatac
     9841 taaagacaaa taaaagaagg gagggtcatt catgatcaaa gaattcaaag agttcatcat
     9901 gcgtggagat gtcttggatc ttgctgtcgg cgttgttatt ggtagtgcgt ttacaagcat
     9961 cgtaaaccaa gtggtgcaag gcttgatcac accactaata ggttggtttg tcgcaatgat
    10021 cacaggtact tctgatttag aaggagcact ttcaattctt gactggtcgc cgacaaaagg
    10081 cgtcacattt gcgtttggta acgttattag tgctatcatt acatttctga tcactggttt
    10141 tgtcttattc ttagttgtca aagcggcaaa ccgtgcgaag aatttacgct ccaaagaaga
    10201 gcaagtggaa gtggaagaaa ctcctactgc agaagattat cttagtgaca tacgcgatct
    10261 tcttgccaaa caagtggcag aagacaatac acatccagta gagcaaacaa atctttccga
    10321 tacttctaaa taataaagaa tctaaaaaaa agaaagccga ttttcagtgt cggctttctt
    10381 tttttagatt cttaacttgc tacttgttcc tttttcatgt cgatatgtgg aatatcatct
    10441 tccaaataca catctgagat tgccttgaat ccaaacgact cataaaaagc ttgtaggtat
    10501 tcttgtgcgc cgatcacgat tggtttttct ggaaaatttt tctggatcat gttaatcgtt
    10561 tcttgaacaa tctttcttcc taaaccatct ttacggttgt cctgatgaac aaggacacgt
    10621 ccaaaatgga tactctccgc ttcttcatat actcgcgtgt aggcaaggat cttcccatca
    10681 gtatcttgaa aaaacgtatg ccaagcttgt ccatcaatgt catcaacttc ttgataagga
    10741 cactgttgtt ccacaacaaa aactgcgatt cgttctttga cgatcgcgta aaactctgct
    10801 gttgacaatt cttcaaattt ctttacctga tattccacta agcaaacgcc ccctgctttt
    10861 ttattcttag cataccgatt cgcacacaaa aaagcaaacg aaccttacga atctcaggct
    10921 cgtttgcttt ttaaactgct tcagttaaaa attcgatctc aactgtccga gtaagtacat
    10981 ctgaatagct gtaagatact cgttcaaaag aattttcttc aggatccaaa tcaacaacga
    11041 atactgaagg ataggtttct gttaaaacgc cgcggcgttc tgtttgacgc tttcttcctg
    11101 tctgagcaac caacatgatt gggctgccga tacgaccttc taaatctttc ttgattgaag
    11161 ctaaagttgt tggcatacag ttcacctcta ctatcaacta taccacatat ttttaaaatt
    11221 ttcaagttta ccataaaaaa aaagaaatgc aataaaaata aataagaagc tcattaagat
    11281 ttttctaaat tattcctcat tataaaattt caagtgtttt tttattttga gttttactct
    11341 cccgtaagcg ctcattactt gcttgtgatc cattgctaaa cattcagcca tgtctacaaa
    11401 atcaacgcct tgcatataat aatgaaaaat tttagcttct atttttgaaa actggatttc
    11461 gcaattttcg attgcttctt ttaataaaag atgctgaatc gctaaaggtt cttgcaatgt
    11521 gatctctgct aaaaagtctg gtccttctgt ctctattttt tggtcgattg agacttcatc
    11581 tttttgatca cgacgcttca gcgcgttctg cttcctcaac agagaataga tttgattgct
    11641 tataatgctt ttataataac ttccaaagct aacacctttg cttttttcgt aagttctcaa
    11701 tgcctgatat aaagcgatcc ttgcttcttg ttcccaatcc tcagaaacat aatttcttaa
    11761 ataataggtg tttttcaaac gtagtaaaag tggaaaatat ttttgataca gggcagcaaa
    11821 tgcttcttcg tcaccattac gtgcagcgcg caataactcc tcagacataa aataattcct
    11881 ccataaattc gtttatggag gaatcttatc atcaatgatt catcgaactg attagaacga
    11941 tatgttattt attttttctc attaaatcgt ccaatttttg ggacaatttt tttaactgct
    12001 cttcattcca tggagaatta cgtcggaaat caataaaatg catatcttta ttttcttggg
    12061 ctattgtttt ttccgttttt tttatcgtct tatataattc ataagcagaa gttcgtaatg
    12121 ctccttggga aaagatcatc cactgttctg ctaaatcgct tgtcgccact gtcacttgtg
    12181 tcaaacggtc attcagctga ccagcgattc tctcgatata actgtcagcc gtttcgtctt
    12241 ctttcgtaaa aacaacatcc aactggtatt tcttataatt ttgttggatg cccggaacta
    12301 gttgggcatc gaagacaacg atgacttcca ttccttcata tttcgcataa tttgataaac
    12361 gatgtaaaag agcttctctt gcatccgcca ttttgtcctg ctttttaagc tggacaagtt
    12421 ccggccacga accaatcatg ttgtagccgt ctacaatcaa cagctgtttt ttcatggtct
    12481 actccccctg acgttgggaa aaagcctgat acataagcaa accagcagca acactggcat
    12541 ttaagctctg gacgtgcccg gtcatcggga tcgtcagcaa ttcatccatt tcttttttca
    12601 atcctggact cattcctttg ccttcattac caatgactaa agcaattgat cctttggcat
    12661 tccatttttg gaagtttgtg ccggtcatat ctgtgccaaa tacccaaaat cctgtttctt
    12721 tgagctgttg cacagcttgt accaaattcg tcacgcgtgc aacagggaca tgttcgactg
    12781 ccccagtgga agtctttgtt acgataggtg tgattccaac tgctcggtgt tttggtatga
    12841 tcacaccatc gacgccactt gcgtctgctg tccgtaaaat cgacccaaaa ttgtgtggat
    12901 cttcgatgct atccaaaatc aaaaagaagg ggtcttcttt tttctctttt gttcgttcaa
    12961 tcagttggtc aagggataaa tattcataag gtgtaatcgc aagaacgact ccttgatgca
    13021 cgccgtgatc gctcaaagtt tctaattttt gtttaggaac ccatttgact ggcacagctt
    13081 gttcacgtgc gatttctttc agttcttcta ctttttctcc tcgtgaatct tcttgaagaa
    13141 agagtttatt ccctcgccct tgttgtagcg cttctactgc ggcatggtgc ccaaagacga
    13201 aattatcttc taatgcttct tcattttgat cagaaactga ctgatttcgt ccagtttgct
    13261 ttttatttga atatctattt tgttttttgt cttcaaatcc acgtttcgat tgatgtttgt
    13321 ttgttctttc tttacccatt tttttcacca actttctgaa tacaccagcg gatcaattct
    13381 tccatccgct cggtttgttt tgtcaaatgc agatagccca tgagcgcttc gaaccctgtt
    13441 gcgacccgat acgtcgtaac atcagcattt tttgcgctcg tatggctttt agcatttctt
    13501 cctctgcgat aataaagttc ttcttcttct gtcaaaagat gttcttccag catttgctga
    13561 atcaaaaagg cttgtgcatt cgcagaaaca taattcgttg ccgcacgatg cagcttattg
    13621 ggttttgttt ctccttgatc aatcaggtgg tcacgaacat agatttcgta aatcgcatct
    13681 ccaacataag ccaaggctaa accgttcaat tgtgtgtaat ctctcatgct tctcttctcc
    13741 atcgggttcc ttgcggtgta tcttctaata agatcccttt ttctttcaac aaatcacgaa
    13801 tttcgtcgct tcggacaaaa tcacgattgc ttcttgcttg atttcgttct tcgatcaatt
    13861 gttcaatctc ttcatctaat aactcttctg ctgtaaataa aataccgaag atcgaaagcc
    13921 attgcgagaa taaagcttct ccttgtgcca agacagcggc agaaactttc ggctggtcta
    13981 gataagtgtt cagccactta gcaagctcat agaccactgt gatcccattt gctgcattaa
    14041 aatcatcgtc catttcttcg atgaatcgct gttctaaatt ttctaacaaa gcgattttat
    14101 ccgcatcatc tgccaaattc tctgaggcag tttcttggcg gaatttcaaa ttgtcaaacg
    14161 tattcttcag tcgttgtaaa ttggtggctg cttctttcat cgtcgtttca ctgtagcgga
    14221 tcggacgacg gtattgtgtg gtcgccatga agaaacgcaa gacttgtgga tcaacgtttt
    14281 tgatcatttc atgtacagtg atgaaattcc ctagagattt actcattttt tcatcgtctt
    14341 cgccaatcgt tacatagcca ttgtgcatcc agtaattagc gaattttttc cctgttttcg
    14401 cttcactttg tgcgatttca ttttcatgat gaggaaattc taaatcttgc ccgccaccat
    14461 ggatatcgat cgtttctcct aaatgttttg ttgccataac ggaacattca atatgccaac
    14521 cgggacgtcc tttgccccat ggtgaatccc aagaaatctc gccaggtttt gcttgtttcc
    14581 ataaagcgaa atctaatggg tcttcttttt gctgctgttc ttcccctgta cgctgacttg
    14641 ctccgacttc cagttcatcg atggattggt cacttaattt cccataattt tcaaatttac
    14701 gggtacggta atacacatca cctgaagatt cataagcata gcctttttca accaacactt
    14761 gaataaatgc taaaatatct ggcatatgat ccatgactct tggatgcagt gtagcaggct
    14821 cgacattcaa cgctttcgta tcttcttcaa atgctcggat gaaacgatca gcaacttctg
    14881 gtgctgtaat acctaattcg tttgctgtac ggatgatttt atcgtctaca tccgtgaaat
    14941 tcgatacgaa atcgacttta tagcctcgat attcaaaata gcgtcgaatt gtatcgaaag
    15001 caatggtgct acgagcattt ccgatatgga tataattata cacagtcggt ccgcagacat
    15061 acataccgac atgtcccttt tgtataggga tgaattcttc tttttttctg gtcaatgtat
    15121 tgtagatttt tatcattctg atctcctcac tttttctcct ttgattcgaa cgacttttgc
    15181 tggtactcct accgctgtag cgtcgtctgg aacatctttc aacacaacgg cacctgcacc
    15241 gattttggcg ttttttccga tcgttaccgg tcctaatatc tgcgcattcg cagagagcat
    15301 ggctccttgt ttaacagttg ggtgtctttt acccgtttct tttcctgtgc cgcctaatgt
    15361 tacgccatgg aataaaatca catcgtcctc gatttctgcg gtttcgccaa tgacgattcc
    15421 cattccatgg tcgatgaaca cgccagttcc aatcgttgct cctggatgga tctcaacacc
    15481 agttaggaaa cgccagaatt gagcattgat cttcgccaat aaaaagagcc ggtgctgata
    15541 taaaaaatga gagaaacgat gccaaaacaa ggcatgaaaa ccagggtatg tcaaaacgat
    15601 ctcagctgtc gaacgtgcag ccggatcgtt tctctttgct gcatcaatcg ctcgggtaaa
    15661 ccaactcatg actttccact cctttcgtaa gaattttatg taggaaatca tcttcttata
    15721 aagaaaagaa gcgcctttta aataagtttc cttatcaaaa gacgctttcc gcgtggttcc
    15781 acttttcttc ataatcaatg attatctctg acagctaacg cctgttatac gttcttggct
    15841 actttgtttc ctataaaaca gtttttcctg ttttctgtgt ttcgccaaga ccactcccag
    15901 aggcatttcg gcaaccattc attgatacct tgcaccaccc ggtatctctc ttgtaatgat
    15961 cagctgctta cttttttctg ttcatcgtgt ttagttcagt acttgattta aatgtgctaa
    16021 tgatttttca cgaccaagca attcgatcgt ttcaggcaat tctggaccat gcatttgtcc
    16081 tgatacagct acacggattg gcatgaaaag attttttcct tttacgccag tttctttttg
    16141 aacagctttg atggcagctt tgacagttgg agcatctact acttccattt gttctagctg
    16201 tgctttgaat gcgtggagaa cagtaggaac tgtttcacca gcaagtactt cttttgctgc
    16261 ttcattcaat acaggatgtt cattgaaaaa tagcgtagac agatcaacga tttctgcagc
    16321 atagctcatt tgaggttgat acaggctaac gatcttcttc acccattcga ttgtcttttc
    16381 atctgggttt tctgctacac gtccatcagc gattagataa ggcagacaca tgtcagtaaa
    16441 tgcatctaga tccattgctt tgatgtaatg gttgtttacc cattgcagtt ttttcgcatc
    16501 gaaagctgcg ggtgatttac ttaggcgttt tggatcgaac attttgatca attcttggtg
    16561 ttcgaagatt tcgtcttcgc ccacaggtga ccagcccaat aatgcaataa agttgaacat
    16621 tgcatctggt agatagccta attcgcgata ttgttcgata aattgcaaga ttgattcgtc
    16681 acgtttgctt agttttttac ctgtttcact gttgatgatc aatgtcatat gtccgaaggt
    16741 tggtggtgtc cagccaaatg cttcatagat catcagttgt tttggtgtat tggcaatatg
    16801 gtcatctcca cgtaaaacgt gagtgatttt catcaaatga tcatcgaccg caacagcaaa
    16861 attataagtt ggcataccat cgcgtttttg tatgacaaag tcgccaccga tattatcgga
    16921 ttcgaacgaa atagcgcctt tcaccatatc tgtaaatgcg tattctgtat tgcgaggcac
    16981 acggaaacgt acaacagatt ctagaccttg tgcttctttt tctgcttgtt cttcaggtgt
    17041 caagttcgca catgttcctg cgtaatgagg catttctcct cttgcgcgtt gtgcttcccg
    17101 ttctgcttct aattcttctt ctgtgcaata gcatttgtat gctcgattgc tagcaagcag
    17161 ctgatcaatc agcggttgat agatttcttt acgttcagat tgacggtaag ggccgtattc
    17221 tccaggattt tcaggagatt catcccaatt cattcccaac caagcaaggt tttccaactg
    17281 gcttttttcg ccatcttcga tattacgttt ttggtcagta tcctcaatac gaataataaa
    17341 gtctccatca ttgtggcggg caaacaaata attgaataat gcagttcttg catttccaat
    17401 atgcaggtgc ccagttggac ttggtgcata gcgtacgcgt actttcgtca ttttcttcct
    17461 cttctttcaa actagacttt gactaacagc aaaaattccg tcagttacgg cttctcaaat
    17521 atcatttatt taaagccact cagcacaaat tttacctttt ctaaggtaaa tagtaaagcc
    17581 aactggttga atttccttac cagttggctg aatttctttt ttattttgat tgtttagtct
    17641 ttttctctgt ctctttttta ttgtcgattc ctcgtcctgc atgtgcaggt ttagcaaaga
    17701 tcatgcgtcc agcagctgtt tgaagggcac ttgttacaac gacttcgata cgttcattca
    17761 tgtaatgctg tccatcttct acgacaacca tcgtgccgtc atcaaggtag gctactcctt
    17821 gttggcgttc tgtcccggct tttactacta gaacattcat cgtttcacca gggatgacga
    17881 ctggttttac ggcatttgcc aacgcattga tattaagaac aggtacattt tggaattcag
    17941 agactttatt caagttatag tcattggtta caatgacacc atctaaaagc ttagccaatt
    18001 tgatcaattt gctgtctact tcactgatat cttcaaagtc tccatcgtac atttcgacag
    18061 atataccctc ttctttttgc aaggcattta ggatatctaa tccgcgacga ccacgcacac
    18121 gtttcaaact atcccctgag tccgcgatgt attgtagttc gtaaagaaca aaattaggga
    18181 tcatcaaggt tccttctaag aatccagtct ttgtaatatc ataaatccga ccgtcaatga
    18241 tcacgcttgt atccaagatt ttatatttat ggaaattttc tcctgatttt cgttctaaca
    18301 gttgactctc aacttgacta gctgtttctt gctcgatttt tttgcttcgt gaaccaaaaa
    18361 ctttcttcca ttcttcgatt cgtgtcgttc ccattctcaa tcccagatag cctaagatga
    18421 tcatcagtaa aatcggcaat acactattga cgaatggaat ttccatattg taaagagggg
    18481 tcgagatcac aacacctaaa gtcaatccga tgatcgcacc gatcgcaccg aagagaagat
    18541 aagtcaagct gacctcactt agtttttgtt ctacttgttt gataatagct gagatatgtt
    18601 tggccaacag caaagataat aaaaagaaaa taagtgcgcc taacaggctg ttggtaaatg
    18661 tattattcag ccatccgttt gactgttgac cgattgcctg ccaagctgag ggtaaaaatg
    18721 aaatacccaa gcttgcgccg acaatgatca tcagcaaagt aatgacacgt ttttgcatcg
    18781 tttttcctcc gtttcgacaa tcatttatta gttagcggaa cacacgcttc aacgtttcag
    18841 ccaaagttgc tactccaact atctcgattc cttttggcgc ttcccagtta cctaagttgt
    18901 ttttaggtag gtatactttt gtgaagccga gtttttggac ttctttgact cgttgttcaa
    18961 tatgactgac acgtctgatt tctccagtca gtccaatttc accgatgaaa cattccgttg
    19021 gctgtgttcc tttttcttta tagcttgaag caatactgac agcgatagct aaatcaatcg
    19081 ctggttcatt taattttacc ccacctgcag cttttaagta agcatcttga ttctgaagaa
    19141 gcaacccagc tcttttttcc agcactgcca tgatcagaga tacccgattg aaatcaagac
    19201 ctgttgttgt tcgtttggca tttccgaaca tagtaggtgt gactaatgct tggatctcta
    19261 ccaaaattgg acgtgtccct tccattgcca caacgatagc agagccagtt gcaccgtcta
    19321 ggcgttcttc taagaaaatc tgtgaaggat tagcgacttc ttccaatcca tgctcatgca
    19381 tttcaaaaat cccaatctca ttcgttgagc caaatcggtt ttttactgct cgcaaaatcc
    19441 gaaaactatg atgttgttca ccttcaaaat ataacaccgt atccaccata tgttctaaca
    19501 ttcttgggcc ggcgatcgag ccttctttgg taacatgacc gacgataaaa atggctatcc
    19561 catttgtttt agcgattttg agtagttctg cagtggtttc tcttacttgg ctgacacttc
    19621 ctgccacact tgtgatgtct ggctgcgtca tcgtttggat ggaatcaatg ataacataat
    19681 caggggagat atgttcgatg gcacgactga tttcgttcat atctgtttca gcgtatagat
    19741 aaaattctgt gttgatactt ccgaggcgtt ccgcgcgtaa ttttatctgc tctgcgcttt
    19801 cttcgccgga tacatataag acttttccgc ctatggctgc taattgctga gaaacttgta
    19861 acagcaaagt ggatttcccg atacctggat cgccaccaat caagaccata gaccctggca
    19921 ccacacctcc gcctaacaca cggttcagtt cttctaattt tgttttgatc cgaggttctt
    19981 ttttagggac gacgtctgcg atttttgttg gttttgcttt ttctcctgtc aaacttgtcc
    20041 gagttctgcg gtccgttgta tcttgttcga tttcttcgac catcgtattc cattttccgc
    20101 aatttgggca gcgtcccaag aacttaggcg acacataccc gcaattttgg caaacaaatt
    20161 gtacttttga tttttttgcc attgtaccgc ctccttttat cctaatcatg agacattaaa
    20221 tcaagcttaa gtatctttaa cgttttattt actggaagaa ccaaatccgc cttgacgttg
    20281 agcagtcgtt tgatcttgat ctgcttttaa aaacggcaaa aagatccctt ggccgatacg
    20341 ctcgcctttt ttgatcacta catccttgaa accaaagttg gtgaattgga acatgatatg
    20401 gccttcattt gcctcattat tgtaataatc actgtcaatc acacctactc cgtttgtcaa
    20461 cactaaaaac cgcttcaacg gattgcttga acggttagca agctgcaaaa attcatcttc
    20521 tcccatgtaa gctttgattc cagtaggcac gagggttggt ttcaattgtt tttcattttc
    20581 tgatacatct tttttcaatc catctgcgac tgtttccaac ccctttttcc agcctgcttt
    20641 ccagatactt ggaatcacga cctcttctgc tgcttcaaaa tcatagccag cagcatgatg
    20701 tgtagcgcgc tgcggtagat tgatattttg atctttatat ttagaaataa cttcaaatcc
    20761 tctttttctc accggttttc ct
//
LOCUS       DAFEOM010000043        20184 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.044, whole genome shotgun sequence.
ACCESSION   DAFEOM010000043
VERSION     DAFEOM010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.044"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            210..1016
                     /gene="proB"
                     /locus_tag="LP524_001793"
     CDS             210..1016
                     /gene="proB"
                     /locus_tag="LP524_001793"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBL2234831.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1013..2260
                     /locus_tag="LP524_001794"
     CDS             1013..2260
                     /locus_tag="LP524_001794"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBL2234832.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2336..2680
                     /locus_tag="LP524_001795"
     CDS             2336..2680
                     /locus_tag="LP524_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234833.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2797..3123)
                     /locus_tag="LP524_001796"
     CDS             complement(2797..3123)
                     /locus_tag="LP524_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234834.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3496..4698)
                     /locus_tag="LP524_001797"
     CDS             complement(3496..4698)
                     /locus_tag="LP524_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL2234835.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            4815..5330
                     /locus_tag="LP524_001798"
     CDS             4815..5330
                     /locus_tag="LP524_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL2234836.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5529..5975)
                     /locus_tag="LP524_001799"
     CDS             complement(5529..5975)
                     /locus_tag="LP524_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBL2234837.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6022..7500)
                     /gene="msr(C)"
                     /locus_tag="LP524_001800"
     CDS             complement(6022..7500)
                     /gene="msr(C)"
                     /locus_tag="LP524_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBL2234838.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7854..8483)
                     /locus_tag="LP524_001801"
     CDS             complement(7854..8483)
                     /locus_tag="LP524_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBL2234839.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8467..8931)
                     /locus_tag="LP524_001802"
     CDS             complement(8467..8931)
                     /locus_tag="LP524_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL2234840.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9418..10686)
                     /locus_tag="LP524_001803"
     CDS             complement(9418..10686)
                     /locus_tag="LP524_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBL2234841.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10917..11654)
                     /locus_tag="LP524_001804"
     CDS             complement(10917..11654)
                     /locus_tag="LP524_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL2234842.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11721..12290)
                     /locus_tag="LP524_001805"
     CDS             complement(11721..12290)
                     /locus_tag="LP524_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBL2234843.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12418..13290
                     /locus_tag="LP524_001806"
     CDS             12418..13290
                     /locus_tag="LP524_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL2234844.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13632..14504
                     /locus_tag="LP524_001807"
     CDS             13632..14504
                     /locus_tag="LP524_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL2234845.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14501..16459
                     /locus_tag="LP524_001808"
     CDS             14501..16459
                     /locus_tag="LP524_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL2234846.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16710..17561)
                     /locus_tag="LP524_001809"
     CDS             complement(16710..17561)
                     /locus_tag="LP524_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL2234847.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17684..18151
                     /locus_tag="LP524_001810"
     CDS             17684..18151
                     /locus_tag="LP524_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBL2234848.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18414..18896)
                     /locus_tag="LP524_001811"
     CDS             complement(18414..18896)
                     /locus_tag="LP524_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBL2234849.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(18996..19859)
                     /locus_tag="LP524_001812"
     CDS             complement(18996..19859)
                     /locus_tag="LP524_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBL2234850.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         6067 a         4156 c         3665 g         6296 t
ORIGIN      
        1 actactccta ttctaacata tccgaggctg taaaagactt tctttacaaa ttcttcaaga
       61 aattctgcta tcttaaagta aaccattaat tcgttctttt tccaaacgac tgcaagttgt
      121 acaaaaaaac tattttttac atgactttac tcgtcaaaac aagtagaata gaacgaaatt
      181 aatgagtgga tatttgattg aggtgtcaca tgcgtaaaga aattaaaaat gcaaaacgaa
      241 ttattgtcaa agtaggaaca agtacattga tttatcccaa tggaaatatc aatcttgcgg
      301 caatcgatca gttagctttt gtcctttctg atcttagaaa tcggggaaag gaagtgatct
      361 tagtctcatc aggagctgta ggcgttggta tgcataaatt acatttgtca gaacgtccga
      421 ctttgattcc tgaacagcaa gctgcggcgg ctgttggtca agccgaactg atgaatatct
      481 ataatcaaag attcagtgta tataatcaac agacggcaca gatcctcgtg acaagagacg
      541 tggtagatta tcctgaaagt cgtaaaaatg tgaggaatac attggaacag cttttgatta
      601 tggacacgat ccctatcgtg aacgaaaacg atactgtagc agtggatgaa ttagatcatc
      661 tgacaaaatt tggcgataat gatcagctgt cagccatcat ggcaaaactt gtcgcggcag
      721 accttttgat cgtcctttct gatatcgacg ggttttattc agataatccg actacaaatc
      781 ccgaggctca gatgtatcgc tatatcgata aaatcgatga aaagattctg gcacaagcag
      841 gtggtgctgg tagcacattt ggaacaggcg gtatgcagag taaattaaaa gccgcttcta
      901 ggatatttga agcaaacagc gcaatgattt tagcaaatgg aaaaaatccg gcaattattt
      961 ttgatattct tgcaggaaaa gaagtaggta ctttatttaa ggaggacttg aaatgactga
     1021 cttgatgcaa ttaggacaac aggcaaaaga tagcgcgcaa caactagcac ttatgcctac
     1081 aaaccgcaaa aatgagctac ttgttcaaat ggcacaaacc atcaaaaaga atcaacaagc
     1141 gatcattgaa gcaaatcata aagacttaga aaaagcagtc gaaaacaata tcagtgaaac
     1201 gatgcaagac cgcttgcagc tgacacctga gcgaatcaat gccatggcta cagagatcga
     1261 aaaaattgct tcgttagacg atcctattgg aaaagtagat gagatgtgga cgaacaccga
     1321 cggattgaga atcggcaaaa agcgcgtgcc actaggcgtg accggcatca tttacgaatc
     1381 tcgtccaaat gtcacgacag atgcagctag tctagcattc aaatcaggga atgccgttat
     1441 ccttcgcggt ggcaaagaag cattcttctc gaatcaatta ttagtccaat tattgcagca
     1501 agtactggtc agtgctggcg aatctcctta tgccatccag tttgtcgatg atacttctca
     1561 tgaaactgca ggacagttga tgcgattgac agaatactta gatgtgctga ttcctcgagg
     1621 cggagcaaaa ttaatccaac gagtcaaaga acaggcaacg gtacctatca ttgaaacagg
     1681 tacaggaaat aatcacgtat atatcgataa agaagcacag ttatctatgg cagtgaacat
     1741 catcgtcaat gcaaaagctt cccgtccatc tgtctgtaac gcggcagaaa cattgctgat
     1801 ccattctgag atcgcacctt ttttcttgcc agctattgaa aaagaactgg tagaacacgg
     1861 ggtctcttta cgtgcggatg ctcgtgcgtt agaatatcta gagacggctg ctttagcaga
     1921 agacacagat tgggatactg aatatctaga ttatatcctt gctgtcaaag tggtcgattc
     1981 tttggatgaa gcaattgcac atatcaatcg gcataatact aaacattcag aaacgatcgt
     2041 gacagatagt tatgcggcta gtcaacgttt cttaaatgaa gtggacgcgg cggctgtcta
     2101 tgtcaatgcc tccactcgtt ttacagacgg atcggtattt ggttttggtg cagagattgg
     2161 tatctctact caaaagttgc atgcccgtgg tccaatgggg ttaaatgaat taacttccac
     2221 aaaatatatc atttatggcg atggacaaat ccgcgaataa tgtttcgtgt gaaacattat
     2281 tcccgccttt tgcctttttt ctgtaaaggt tctatccaac tagaaaggtg tcaatatgtt
     2341 tgaaatttta atgatttttt ttatttacct tatttctctg aatatcgctg cttttctggg
     2401 agtcggtatt ttatcccttt tctttcaatt caaaaaacga agcataggga gtcaacggga
     2461 aaaatggtcg cagtattttg acaaaatcgg gcaaaaaggg ctggttacac gcttgcacat
     2521 tagttacatg gttgcattat gcttattagc gacaatcaat tactattctt ttttcgatca
     2581 ttcaatcgct tatacgatta cgctactgat tgctgggatt tttcacttat cttataaata
     2641 tcagctgaat aaaaaccact tgaatcggac tttccgctaa aaaatatccg tacgataagt
     2701 agagtgcctt gaagatcggc aaaaaactgc cggacttttt agaggctgtg acaaagattt
     2761 tgtcacagcc tctttttcca tataaacggc gttcgcctac cagctgcctg gtaaatctgt
     2821 ttcctcttcg ttaagaagcg aaaatccgtc aaatgtttcg ccatcataat aacgaagcag
     2881 ctcatccatg gacaagttca tcgttttttc tttttcttct tccgctgatt cagccagtag
     2941 ctgttcccga tacacctgat acataccagc tattttttgc tgcatcgctt ttctagtttc
     3001 accttccgca ttgattattg gcaattcttt gatatagcca cgataaatca accgatcatc
     3061 tgaaaattca atgggtaatt caacgagtgt caacccgact tcctgaaaat attcttccag
     3121 catctattat ccccccattc tttttcttaa atgaatttta gcttacctac tgaaaatttt
     3181 ccagtttcat tattttgaga aaaaaaggga atgtgacaaa aatcgtgtca catccccttt
     3241 ttccgaataa atggtgttct tctgccaact cctcaatcat aagtcacaat attcaacaaa
     3301 catgagaaac tgttttttga aattgttcct tgtgattcag agttaaacga cagtctcaca
     3361 acctcttggt aaacggcgtt caaaagcttg catctgcggt aataagccca accaaacgaa
     3421 aaatatgaga agctattttt gtttggctgt tcttatgact tgatgcagga cagctttttc
     3481 acaacctctg atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat
     3541 acaacataca ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat
     3601 atgttcacca atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa
     3661 actatagctg gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt
     3721 caacagtgta ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag
     3781 gactggcata gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc
     3841 tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga
     3901 agcaatagcc agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata
     3961 gccaatcaat aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa
     4021 tgcgattagt ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag
     4081 gcctactttt ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc
     4141 gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac
     4201 cccaccgact agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat
     4261 tgctttttct gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa
     4321 taaagcattc ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga
     4381 taatccgcca agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttt
     4441 caaaccgatc cgactagata agaaacctgt aaatagcatc accacaccag tgactaacat
     4501 gtaactagta aataacaggg tcgtttcagc tggcgtagcg tttaactgtg cagcaatcgt
     4561 ttttaagatt gggtctacta gacccactcc cataaaagcg atcatacagg tagctgctac
     4621 aactagcgct gaacttttcc cctttgtttc ttcatttgtt tgttcttgta tatctatttg
     4681 attctccaat atttccatcc ctttctctct attagcatca tacatgattt attttacatg
     4741 taaatatgca catgtaaaac gtggaaactt tactgtgata taataaagaa aataagacga
     4801 gaggtaggat ttttatgaat actttagcct atatattatt atgcgtgctt ttatccaaac
     4861 cacgttctgg gtatgaattg aagcagctga tcacaatttt ttgggaagcc catcacagtc
     4921 agatctatac aacgcttgcc aagttagacc agcagggtta tgtgaccgtt ttggacaaag
     4981 acgagcactc tcaaaaaaag atctaccact tgactgatga aggacgtgct cttgtagagg
     5041 aatggatcaa agaggaaact cccgctccta cgcaaaaaga tgaatttcta gcaaaaatct
     5101 atgctttttc gacgctcgat aaaacaacag ctactggact gctattcact agagaacaac
     5161 agctgaacaa aatcatgcaa aaaaatcaaa aaaaattgaa tgacctctcg ctagacaaga
     5221 aacaagaatt tggacgttat gttgtgatcc agcgcagaat tatgctttgt cagcaggaac
     5281 tccaatggtg tcagcaagta agagaacaaa tgcagcagtt ttttgagtaa gggataaaca
     5341 gaacctttct agaaatatct attgaagagc aaattatctt caataaaaga gcaatacagt
     5401 tggaaaagaa aaagcgatcc gaacgacagg ccaaaaccta tcgttcggat cactattttt
     5461 ctagaactgc attcaagaga ctatgacaaa aagcatgcca aggccttttt tcaaataaca
     5521 gtgttccatc attcattact ttgaattgct tttagcacac ttcttgaaag cgggccaacg
     5581 atgatcaact gcaacggtaa agcggcaatc acattcattt tccaagcatt catataagca
     5641 gagaataatt gatcaggtat tccttgttcc atgatcacac cgaataaaga catacatgtc
     5701 accattccta aaaccatcaa gcatgaaata gttaagatca tttggacctt gctttctgga
     5761 tttactggta gtttaaaggc aatttttttc gccagcacac cgatgatgaa gacatccaaa
     5821 atgaaagcca ccacgaatcc aggcactaga ccaatcaaca aacttgataa atgaagatct
     5881 tgatgcagcc ataaattata cgcgctcatc cccaagacca tcaaaaaaca catcattgtt
     5941 gtaaaaatta ttccttcttt tttatttgta ggcaatctgt accactcctt taaatttgtc
     6001 acgaaaatca tcgtagcaca tttaaaaatt tctcgtaagt acttttttta ttttctgccc
     6061 ttttatttca taaacttcat cagccgtacg ctcgacaaag taagaatcat gagaagtaaa
     6121 caaaatcgtt cccggatata tttgcattag cttctctaaa gcttcgatcg ttgccatatc
     6181 aataaaatta gtcggttcat ctaacagcaa cacattactt ggcttagtaa aaagcaaagc
     6241 aatcgtcaga cgggtcgctt ctcccccact taatgtacat aacggtttcg tcaaagtttc
     6301 ctgagcaaac cctaagtgat tcagcaaact acggacaagc gattctgaaa actctgtttg
     6361 tctcatgaca aaacgaatga tggattcttc agatgtcatt tgataaccct tttgctgata
     6421 tacttgaaag cttactttcg gagagagaag gattccttct ccttgtttgc ggatatgttc
     6481 taataaggtt gtcttacctg aaccattttc accgacaatc gcgatttttt tacctaaaga
     6541 aaactgaaaa tcaccattta ccagtaatgt tctcccactt ctttccaatt ggacattttg
     6601 tcccatgatg ggatagcggc tgtggagttc aagagatttt ggtactggaa aacggatttg
     6661 tttcatctgc tgcggcttgg ttgtttctcc tattttttgg agtcttctct ctaatgtttt
     6721 cgcattcttt tggatggctt tttgtacact gtctttttgt ttagaggaag acaaccgatc
     6781 cggtctgatt tgttgctttt tctttttact ggacactttt tccaactttt ccgcttgttt
     6841 tttcttttct tggatcgatt tttccaatcg ttttttctct ttcatgatcg actctgcttc
     6901 tcgtagctga gtctttttct ccaattcctt ttgagtgaga taggcactat aattccccga
     6961 aaactctcgg atttctccgt cttttacttc ccagattttc gatgctagct catctagaaa
     7021 atagcgatca tgactaacaa aaaccagcgt accataataa taacgaagct cttcaatcag
     7081 tcgatcaata ctttttctat ctagatgggt tgttggctcg tctagaagca tcccttgttc
     7141 ataagaagaa agaagacgta tcaactcgat ctttcgttct tcccctccgc tccatttccg
     7201 ctctgaagtt tcttccagtc ttaactggaa atgactggct aattccaaat ttaggtcctt
     7261 tgcttcagcg agataggtta attgaggcag gtagttgaag gtgatctctg tttggatttt
     7321 cccatgatcg ggaaaaagtt caccggctat tagtttgagc aaagtcgatt taccgacacc
     7381 attttctcct atgatcccta tacgatcttg ctgatagaca cgaagagaat caatagatag
     7441 ttctagctgg ttgccaaagc tgacttgtag atttgttata tttactgcta aattttccat
     7501 atgttttaca ctcctatttc atgtaaaaca acaattatcg atactaaaaa agagcagaaa
     7561 aatctgtttg caaaaaaact gcatcacatc ttttcctact ctgaacgaaa taactatgac
     7621 taaatcacca aatctgtcat agctttcacc tattctactg tcgttcgaaa aaatggacat
     7681 actccatcca tgaaattgtt gtttatacat gtcttttgaa tttatttagt tgttattcaa
     7741 aagttcgaaa cgtaatttca tcgatgcagt catttttccg acctcctttt ttattaggtt
     7801 accacacacg attcaaaaaa agcaatagct actgaatagt aactgaaaac cttttattcg
     7861 aacgtagtga tcttttctgg tgctaaacga acagactcgt aaccttcttc taccgctttt
     7921 cctattgata aaataataac tggaacataa cggtctttat ccaaaccaaa tgcctctgcc
     7981 aactgatctg cttcgaatcc accaattgga tttgtttcat agccatgatc acgcgcaact
     8041 aacatgaatt gcatagccgc taagctagaa tcgactttga ctacatcgtt catttcttct
     8101 cttgaaaaac tcttgtaata aggaatgatc gcagctaatt gttgatctct gacttctttt
     8161 ggcatcttcc ctgattcgta agcttggtta tagatttctt cgccatattc gtaacattgc
     8221 atatcgccaa aaatcatgac cattgcagaa gaagtatcat tttgtctcgt attgaaacgg
     8281 atcaaaggac gcaagatttc ttttgcttcc tcgctttcag cgaccacaaa gcgccatggt
     8341 tgcatattga cagaagaagg tgcagtcgtc gcttctttga tcatttccag catttcttcg
     8401 tgtgggattt tgtatgtttc atcatatacg cgaacggatc ttctaccagt cataattgtt
     8461 gaaaaatcat ttttttgttc cattattatt aacctctttc gtttctgttg ttatattgca
     8521 aagttgggta ttcaatttat ctaaaagtgt tagaaattgt ttcatttcta catcgctgaa
     8581 agccggggat agcacggaac ctgtatcttt atggtccatt tcacaatgct cgattttctt
     8641 ctgggctttt tctgttagcg ctacatacac ttcccggttg ttctgaggat ttctctgacg
     8701 cgaaacatag ccatttgctt ccaatacctt cagatgacga gtaactgctc cttgatcaat
     8761 ctgcaacttt tcttgcaaac tgctttgtag caatggattt tcttctttca gtctcatcat
     8821 gatctcgtat ctagtaagac ttataccgat attttcttca aagatctgga cgattttttg
     8881 atcggctaat ttgatttgat ataaaaggtg actaatctct tctaaagaca taatttcctc
     8941 ctttctaata attgactcgt caacgattga cttgtcaata atagaataaa aaaagaaaaa
     9001 agtcaaagaa tcagctgtgt aataaaaaaa acgctctcaa tagaaaagta tagagaaaag
     9061 aaattttttg tagaatgaac ggaaaaaatg ataaagaaag cacaacgcta aaaccaagat
     9121 tattgattct tatcaaacta tagaagatag ctcttctgca gtagaaaatt ttacaccgcc
     9181 aaaaatttcc gaaatcgcct tttgcaagaa tcccgtcgaa tattagaaat tggatcagta
     9241 tgcttaaaca gtaataaaaa agttatgaca agatgttgtc acaacctttt ttgagtaaac
     9301 ggtgttcaaa aacttgcatc tgcggtaata agttcaacca aacaaaaaac atgaaaatct
     9361 gtttttgttt ggctgttctt actacttgat gcagggcagc tttttcacta cctcttttta
     9421 ataaccataa aatcctattc cttcatatct ccagcccatc ggtgttagcc aatctctttc
     9481 cttagcactc aaggtatagt gatgggtacc agttttttca tttggattaa atagtcgata
     9541 aattggcttg gaaccaccag aataaaaact tagaccttca taacgccaac cgatttttgt
     9601 taaagagttg atctcagatt gtgccattgt gtaatgatgg tctccactgt aaggattgta
     9661 taaacggtag acaggttgtc cgctgtttgg tgcttgccaa gcaatccctt catattccca
     9721 gcctacagtt ggcagataat ttttttctcc ggcattcaat gtatataaat gttctcctgt
     9781 atgacggtta tacagacgat acatcggaga agttgaagtt ggtgctgtat catactgtgt
     9841 cagattatag gtttcgatta cattgttcaa tgtagaacca taattaggcg cagtggcata
     9901 acgacctgtt agccaagccg tagcatcttt gtaagagttc gtattgcttt tccatgcacc
     9961 ggaatagtag taaacgcctg gctgaaaaga agtcgtcttc aataccgtta cgttatcttg
    10021 caatgattct ttgtacgaag gatattttct aaaagcggca ttcaccgtaa tccattgccc
    10081 attcacatat tccgaagtag gcatcgtaac agattggcca ttatagcttc ctttaatccc
    10141 aaatagattg taattagggg cagaagccaa agtactgttc ccccagccac tttcaacgat
    10201 ggcttgagca atcatgactg atgcatataa gtcattggca cttgccaaac tttgtgcagt
    10261 tgaggcaata ctatcaatga acgcttgttg agaattagca gatgagcgag cataaaaagc
    10321 atagccagta tagttttcga attcagacaa atctgcttga tgggtatgtg tcaatgtttg
    10381 cccatctgtc aattggttcg tgatttcttc tgcattctct ggattgttct gagcttctgc
    10441 tgatgtactg tcttcggttt gtgtttgttc tgttgtttca gttgttggtt cttcggttga
    10501 aattgtattt tctgagacag tagctgactg caaagtactc tctgtatcgt tttcaattcc
    10561 tgtttctgaa gaactcgatg tctgagttgc gacagtttct gtcgattctt cagctagcac
    10621 tgttgtgttg gcaactgcta gatttcctaa aagaataacg ctaaataata aattctttgc
    10681 tttcatcttt tttctccttt gaattatgaa acttttccta aataacagtg taactaaaaa
    10741 agtaaaagca agtcaatgtc catatttaaa taacatttgt ttctttttga tgaatttgct
    10801 ttttctacat aaaaagacgt aaatgaagaa tattcttctc cattcacgcc ttaaattgat
    10861 ggtcgtttga ataactattt aacaagtaat tctctttgga tattatccgt tatggattat
    10921 acttcctgtg tcgtcggacg gataatcatg ttactgacag acatccggtc tggcgtttct
    10981 atcgcaaatg ctacagcaga agctatatct tcggaggtca atccccattc tttttgcgct
    11041 tcatgaagtt caagagcagc ctttttgtcg ctgatggttt gatataattc agtttgaaca
    11101 gctcctggag agatgatcgt agatttgatg ttattttgcc gttgctcttg acgtaatcct
    11161 tccatgattg cacggacagc aaattttgtt ccgcaataaa cggcggaatc aggataaaca
    11221 acatgtcccg ccacagaatc agttgaaatg atatgcccag atttttgttt ttccatgatt
    11281 ggaagtacgg cggaaattcc atttaacaca cccatgatgt tgatatcaag catattttgc
    11341 cattcatcac ggtgaccttc aactaatgga gcggttggca tgattcctgc attattgtac
    11401 aatacatcga ttcgaccgaa cttttccatc gctaaatcga taactgcttg gacttgtgca
    11461 aaatctctta catctgctgt ttgaatatat agctcagctt ctggtaaaga ttcttttatt
    11521 gcttttaggc gatcttctcg gcgtgcagct ataactagtt tagccccttt ttctgctaat
    11581 agcttcgttg ttgcttcacc gattccgctt gaagcgccca tgatgacaat cactttttct
    11641 gataaatcat tcattctatt ctcctcctgt tttgaaaaat ctcttgctta tactcactat
    11701 ctcttatttc atcttactgt tcaaataatc cgttcgacag gtttgacgat tttggcaggg
    11761 attcctgcaa cgatggtatt gtctgggaca tctttcgtaa cagttgcatt cgctgcaaca
    11821 atggcattct cgccaatcgt tacgcctggc agaatagttg ctcctgctcc gatccaagca
    11881 tttttcttga tgtggattgg ctttactgac aagcctcttc gttcttttgg atcggttaga
    11941 tggttcaccg tgatgaggtt gacacgagga ccaatcaata ctgaatcttc taaacagatg
    12001 ccacctaagt caacgaacat cacttgacga ttgataaaga catttttccc tatactaata
    12061 tgtttgccga aatctgtata aaaaggcagc agaacagtca ccgtatcatc tactttgtca
    12121 ctaatgatat tttctagccg tttgcggata tctgctggtt catggtaccc agtattcagc
    12181 gctgctaaag gctcttcgtt ttcagcttgg atctgatgga tcacttcaaa gagcggatcc
    12241 gttactcgaa tctctttatt gacgatttgt ttttcgatga ggcttgtcat tttgctccct
    12301 ccttttatcc ttactttata acggacggct tatcatgtct aatacttata aaacatactt
    12361 aactattcct tttaactata cttagtggta tgctttttta aaaacaggag ggaaaaaatg
    12421 gatattcgta tactgacgta ttttttagca gtagctagag aaaaaaatat cagccgagca
    12481 gcaaaatctc tgcatatcac gcaacctact ctctcaaaac aactgaaaga tttagaagaa
    12541 gaacttggtg tgacactttt cactcggggc tcccgagaga tacagttgac ggattctggt
    12601 cgatatcttt attcaaaagg aaaagaaatc ttgtcgttgg tagaaaaaac ggcgaacaat
    12661 ttaacaaatg acgcaatcat ctctggagaa atctacattg gcggaggcga aactaaagca
    12721 atctctttca tttctgatag ccttcattcg ttgatcaagg cgcatcctga tatccagttt
    12781 catttatata gcgggaatgc agatgatatc atagacaaac tggatagcgg attacttgat
    12841 tttggactcg taatcgaacc gacaaacaaa caaaaatacc agtcatttcg actgccagcc
    12901 caagatcgtt ggggactttt gatacatcga aatcatcctt tagcaaagca aaccgtcatc
    12961 catccgcagg aactgaaaac tgttcctttg atcatttccc ggcaaacgtc agtggacagt
    13021 caactagctg aatggctagg tttcagcata gatcagttat cgatcgtcgg aacatataac
    13081 ttattgtaca atgcttcttt gatggtcgat aacgggactt ttggtgcatt agctctagat
    13141 gggatcatca atacttatgg gacggaaaaa atattcatcc cactcgatcc gccattacaa
    13201 gcttcactga atctcatttg gaaaaaagac cagcctcttt ctcctgctgc tcgtaaattt
    13261 ttagatgtcc taacggagaa atacagttaa gtagagtttt tttccttcgc tttcagcttt
    13321 ttaacaacga ataagataag caaaagatcc aacgattaaa taacataagt ttcttaattt
    13381 atttcttcct tttatttctg ataattccag taaattacta taattataaa aaaagaattg
    13441 acaaaacata actgtctata taaaattgat tacgttaaca ataattcgtt tcatgtgact
    13501 agatcatact aggcatgaaa aatctctgag gaacatggtt tccctaggag gtttttcgtg
    13561 tctatttttg tttggccgca atcaactggt ctagcaaacg tgaaaaccaa atcttcaaaa
    13621 agggtgcgcg catgaaaatc aaaaagatct ttaatcaaaa tgcagtattg gtagatgacg
    13681 gtggacagga aaaagtagct attggaaaag ggattggatt tgacagaaaa agaaatgatt
    13741 tgatttttga tcgtgatgtc gaacggatat ttgtcatgga accagaaggt caaatcaagt
    13801 tgcaaaattt acttaatcag attgatgaac gctttttatt tgcagcagaa acgatcattg
    13861 atcatgcgga aactgttttg atggaaaagt tgaatgaaca tgtattgatc gctttgaccg
    13921 accacattgc tttttctgcg gagaacatta ataacggtat cgtcatccgc aacaaacttt
    13981 taagagaaat cgaagtgctt tacagtgatg aattcagtat tgctcaatgg gcagtagagt
    14041 atctgacaaa agagcttggg attccatata cttatgacga agcaggatat atcgcaatcc
    14101 atattcacag cggacgaagc ggtcggaaca ataaccatcg cagtatccgt gaagtgacga
    14161 ttgtatctga catcatccat ttagtggaat cagaattgga aacagatatc cattcagaac
    14221 cattttcact gaattattca agactggtga atcatttacg actacttcta caacgaaccc
    14281 atgcgcaaca atatgctgtt ttagacacgg aaatcgtaga aatggtcaaa cgaaaatacc
    14341 cagaaagcta taagatttct aaaaaaatcc gtgtgttgct gaccaaagaa taccagctcg
    14401 cgatcacgaa agaagaactg ggttacttgg ccatccatat cgagcgttta agaagcgcaa
    14461 tcgtacaaac aaatgttaac aatcatgagg aggaaaaaaa ttgaaagcat atctacaacg
    14521 aatgggacgt tcccttcaac ttccagttgc ggtcttacct gctgctgctt tactagtagg
    14581 gatcggaaac tggtgggcat cttatagtaa cgacattgtc gctcattttt tacaagctgg
    14641 cggtaacgca gttttaggtc aattaccttt gctgtttgcc gttgggttag cactagggat
    14701 gtcagtggac aaatcaggtg ctgctgcctt ggcaggtttg gtcgcatttg aagttccagt
    14761 aaatgtttta aaaactgatt cagtagctac tttattgaat atcaaaccag aatcagttga
    14821 tccatctttt gctcagatca gtaacgtatt tattggtatt attgccggat tgattgcggc
    14881 agctctttat aatcgtttcc acgaagcaaa attaccgatg gctttatctt tctttagcgg
    14941 caagcgtcta gtgccaatct tggctgcctt tgtcatgttg ctgatcagtg cagtgctact
    15001 gctcgtatgg cctccggttt actctgcttt agtttctttt gctaaattca ttttgagttt
    15061 aggctggggt ggtgcaggtc tttatggctt cttcaaccgt ttactgatcc ctacaggatt
    15121 gcatcaagca atgaactccg tgttctggtt tgacgtagca ggaatcaatg atattggtaa
    15181 atttttggct tctgaaggaa caaaaggcgt aacaggaatg tatcaagctg gatttttccc
    15241 aatcatgatg ttcgggcttc cagcaggagc atatgctatt tacagaaatg cacgtccaga
    15301 aaacaaagca aaaacagctt ccttgatgct agcagcagct tttgcttcat tctttactgg
    15361 tgtgacagaa ccacttgaat tttcatttat gttcgttgct tggccattat atgtcttgca
    15421 tgcaattttc actgggattt ctttagcagt gagtgcattc ttccattgga cagcaggatt
    15481 tgcctttagt gccggatttg tcgattactt cctatctttg aaaaatccga ttgcgaatca
    15541 gccattgatg ttgatcgtcc aaggattagt aacagcagtg atttactact ttggctttaa
    15601 ctttgctatc aaaaaattcc atctgatgac tcctggccgc gaggaagcag atctttcaga
    15661 tgacgatact gcaacgacta attcagacaa taaatatgcg gcacaagcaa gcaaaatcta
    15721 cgctgcatta ggcggagccg acaatgtaac ttcgatcgat aactgtacta cacgccttcg
    15781 tttgcaagtc aaagatacag gcctgatcga tcaaaataaa atcaaagcca ctggcgtacc
    15841 tggtatgaag atcatcgacg gaaaaaatgc gcaagtcatc gtaggtacag aagtacaatt
    15901 cgttgcagat gagatggcaa aactacatgg tggtgctgca gcacgacctg cacaaacaaa
    15961 caccgtggtt aaaacagaaa catttgccgg tgaaacgatc gaacaagata tttacgctat
    16021 cgcaaatggg aaactgattc caatcactga agtttcagat gatgtattcg cggaaaaaat
    16081 gatgggtgac ggctatgctg tattaccaga aaatggagaa atcttctctc caatcgctgg
    16141 tacgatcaca aacattttcc caacgaaaca tgctgtcgga atccaaacag atgctggtat
    16201 cgaagtcttg cttcatatgg gcatcaacac tgttgactta aaaggcgaac cattcacttt
    16261 atatgtagaa gaaggtcaaa aagtcgcaag aggtcaattg attgctttag ttgatcttgc
    16321 cgctattcaa tctgcaggca aaaacacgga tatggtcgtt gtattcacaa atggcgacaa
    16381 agtccaaagc ttagaaatcg aacccgcacg tgatgtaaaa gcaaatgata aaatcggctc
    16441 tgtttccaac aaagcctgag ccatcgtctt ccccagactt ttttatcgat aaacaaacag
    16501 gagctgagac aaaaatcttg tctcagctcc tttttccgaa taaaaacggc gttctcctgc
    16561 caactccgcg gtaataagtc cagctaaacg aaacatatga agagctattt tttatttgac
    16621 tcttcttatt acttgatgca gaacagcttt ttcacaaaat ttttcttcat actcaaaact
    16681 aatcggcttt ttcacaacct ctttatacat tattcctgat ccaatatttc ttttaatgct
    16741 gcttcaagag aagttaatgg ttttcccaat actttttcta aatcatcaga ggaaatagct
    16801 aactgatcct ttttaatatc atgttggatc gctaaaaaca tgtcagctgc ttcttgaggg
    16861 aatccggctt cttttagatt ttcgacaaac gctgcatcac ttgcttcaat aatttctaac
    16921 tctttacctg tcgcacgctc taatgccttg gctaattctt catagtcagt tagattgccg
    16981 gataattcta agatttctgg agagtcactt tctactagta cttttgctgc agcttctgca
    17041 tattcacgtt tcaatgccca acctgttttt ccatttcctg cagcataaac gaattgacca
    17101 gtttttaaag cctgcccaat gataggtaat tcattctcta cataccaatt attccgtaaa
    17161 aatgtatggg aaatacctga atccttaatc attttttcag tgaattggtg atctggcgct
    17221 aatacactga tcgagtgatc ggcatctgca aaacttgtat aagcgatata agatacccca
    17281 gctgcttgtg ctgccttcac tacattctta tgttcggctt gacgatttcc tggaacgcca
    17341 gagacaaaaa gcaatcgatc tattcctttc aacgcttctt tcattgagtc taaatcatca
    17401 taatcaccaa tacgtatatt aaatccagct tctttcagtg cttctccttt ttcttcacta
    17461 cgtactagta catagatttc attttgtgga accatctttt ttaagaattc cagcgcataa
    17521 ccaccaaaac ctccagttgc tcctgttact aaataatgca tatttgccac tccctattct
    17581 ttttatttgt taagatttac agctgtacat ttataaatta cacctttatc ataaagagtg
    17641 tggtaagata agtcaagcaa aaaaaatgaa aggatgacaa ctgatgaagt attctctgca
    17701 atttagtgat gcaatccatg tacttgccta tattgaaatg tttcaagata cccgatcact
    17761 ttctagtgag atgattgcca gtagtatcga aacaaatgca gcaaatgtac gtaaaatcat
    17821 gagtcagctc aaaaaatccg gtttgatctt tacaaaaacc ggaaaaccgc agccttccct
    17881 tgccaaaagt ccagatcaga tttctttact agatgtctat cgcagcatcg aaggtaatac
    17941 acagcttatc caagtcgatc caaaaacaaa tccaaattgc gtcgttggtg cgaatatcca
    18001 agaagcttta gaagaaagct acgctcggct gcagaaaaag gcagaagaag aaatggctaa
    18061 gatcacactt gatagtctga tacataaaat agccgttttg gaaaaagaaa aaagaccaga
    18121 aaacacaggc ctcgtagaga gatttttata attctctcta ctgtgcctgc atttttctga
    18181 tcggaatcgt ctacataaat tcttttattc tccgggggac aaaagccttg tcatgcccct
    18241 ttttcctctc taatgctcag gtcaaagcgc tgaagtcatg acgtcttgat aaacggtgtt
    18301 caagtagctg accctcggca ttagttctga aaaatgcaaa atatggaaag ctatttccca
    18361 tttttcctct ctaatgctca ggtcaaagcg ctactttcac gacctctata ttatcatggt
    18421 ttattgacta ccggtatcca gatttctccc atatatttcc cgttggtaac tcccatttcg
    18481 actacagcat ttggaccccc aacataggcg taatcttcta cttctcttaa aacatgccca
    18541 aaggccgtat tcgtcaaatc atttcttaat tcttcaggcg attctgctaa agcgcttaca
    18601 acaagatacg ttccttttgg gaatgggatg attcgcgtag tgtttggaac atcctgactc
    18661 gtttgaacac cgacatagta catcattttc ccgttatagg gttcgttgac tgtaaagaaa
    18721 taatgatttt cagcagcttg tttcaatgca tctattgttc cattaccgat aactttttgc
    18781 tcaaatgctt gtttctctgc agccaaaccg acaaaatccg tattgctact cttcaattcc
    18841 acaccaatac ccgtcaccgt aaaatctggt ttttcttcga tcctatactt tgccatttct
    18901 cccacccttt ctattatatt aagttcatct tacataaaat taaagggaaa tgacacgatt
    18961 acgctctttt ttctagggat atttttattt tttaactatt tttttgcttt cactgcaatc
    19021 caaatctcac tgtaaacatt actgaaatcc tctccccatt ttgtaaaaga gatctcggga
    19081 gcttcgacta acccataatc tgaagaagga agccattctg catagatttt cccccaagta
    19141 tcttgtaaag tttggggaaa tggcccttta ttaggaaaaa tggcccaagt gtgttcttct
    19201 atagcaagca tttctaaatc agcgtacgga ttttcttttg tcgtagcaaa gccaatcatg
    19261 tgcatcaaac tgcccttttc ttccaagcgg tctccatcaa atccataaga aacgttgatt
    19321 acttgatttg gatataaatc tcctaactta tgcatctctt ccctttgagt ctccgtgatc
    19381 gtctgcgcta acttttgtat ttcttgattg actccttcaa atttgacagg aacttttttc
    19441 gccacacctg ccacataaaa ctgttctttc ttttcgatac gaaattccat cgattttcct
    19501 cctctgacat caataaaaaa tgtcaagcgt ggaaaagatt tttgtatgcc gtatctgctg
    19561 atttctgaag gcagatatcc tgaccaatca cgaaaagctc ttgaaaaacc ttcgactgtt
    19621 tcatatccat atctaaacgc cgtttctgtc actcttgatc catctagaag atcgcgatta
    19681 gccaaagcca atctacggtt tttgatatag tccattagcg acatccctgc aataaacgta
    19741 aagatgcgtt ttaattgata ttcagagatt cctgtatttt cggctattat tttataggaa
    19801 agtccttctg tcaaatgttc ttcgatgtaa tcgatcaatt ggttcagatc tttaagcata
    19861 tttatcttcc tccttgattg cctttttatc atattatcaa agaaagtcaa ttacttgatg
    19921 aatcaggaac caatttagtc agatgtagaa aatgttgtat ggatctaaag aaaattctca
    19981 agcagcgttt cgtaacggat aattggtatt atctgcctat ctatgtttta cctttagaaa
    20041 aataaatttt ggggaagaca gattctaagt aaacaaagcg gtaaaaatat cttttattcc
    20101 cttagttaat ccgcttacta cctactgtac aaagcaaatt gttgttcaaa ctgctgatca
    20161 gaacgctgct tggatttttc tttg
//
LOCUS       DAFEOM010000044        19995 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.045, whole genome shotgun sequence.
ACCESSION   DAFEOM010000044
VERSION     DAFEOM010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.045"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            4..816
                     /locus_tag="LP524_001813"
     CDS             4..816
                     /locus_tag="LP524_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBL2234851.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="LP524_001814"
     CDS             813..1613
                     /locus_tag="LP524_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBL2234852.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="LP524_001815"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="LP524_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBL2234853.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="LP524_001816"
     CDS             3110..4819
                     /locus_tag="LP524_001816"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBL2234854.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9383
                     /locus_tag="LP524_001817"
     CDS             5031..9383
                     /locus_tag="LP524_001817"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBL2234855.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9527..10000
                     /gene="rimP"
                     /locus_tag="LP524_001818"
     CDS             9527..10000
                     /gene="rimP"
                     /locus_tag="LP524_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBL2234856.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10024..11199
                     /gene="nusA"
                     /locus_tag="LP524_001819"
     CDS             10024..11199
                     /gene="nusA"
                     /locus_tag="LP524_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBL2234857.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNSKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11221..11514
                     /locus_tag="LP524_001820"
     CDS             11221..11514
                     /locus_tag="LP524_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBL2234858.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11511..11822
                     /locus_tag="LP524_001821"
     CDS             11511..11822
                     /locus_tag="LP524_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HBL2234859.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11835..14141
                     /gene="infB"
                     /locus_tag="LP524_001822"
     CDS             11835..14141
                     /gene="infB"
                     /locus_tag="LP524_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBL2234860.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14168..14515
                     /gene="rbfA"
                     /locus_tag="LP524_001823"
     CDS             14168..14515
                     /gene="rbfA"
                     /locus_tag="LP524_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBL2234861.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14623..14844)
                     /locus_tag="LP524_001824"
     CDS             complement(14623..14844)
                     /locus_tag="LP524_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL2234862.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15292..17655
                     /locus_tag="LP524_001825"
     CDS             15292..17655
                     /locus_tag="LP524_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HBL2234863.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKLGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRNDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFHSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAATLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            18023..18946
                     /gene="truB"
                     /locus_tag="LP524_001826"
     CDS             18023..18946
                     /gene="truB"
                     /locus_tag="LP524_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBL2234864.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18950..19891
                     /gene="ribF"
                     /locus_tag="LP524_001827"
     CDS             18950..19891
                     /gene="ribF"
                     /locus_tag="LP524_001827"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HBL2234865.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKKDEQATAEYFSQQ"
BASE COUNT         6862 a         3512 c         4389 g         5232 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc
     5701 cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac
     5761 ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga
     5821 ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa
     5881 aagatgaaca agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta
     5941 ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca
     6001 aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc
     6061 atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag
     6121 gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg
     6181 aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg
     6241 tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca
     6301 catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg
     6361 agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg
     6421 atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg
     6481 ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata
     6541 caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa
     6601 ggtatggtat tcctgaagcg gccaatccag tcattgatac gttggaatta gctagatatc
     6661 tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct
     6721 tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct
     6781 ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata
     6841 tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa
     6901 atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg
     6961 aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg
     7021 gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg
     7081 caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc
     7141 ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt
     7201 tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc
     7261 tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc
     7321 cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg
     7381 catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa
     7441 tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc
     7501 caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag
     7561 atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta
     7621 atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg
     7681 gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa
     7741 tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt
     7801 tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat
     7861 gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc
     7921 atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc
     7981 ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta
     8041 cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc
     8101 agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa
     8161 ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta
     8221 cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt
     8281 tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg
     8341 ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc
     8401 cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt
     8461 attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt
     8521 tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg
     8581 gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg
     8641 cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata
     8701 gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg
     8761 atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata
     8821 tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat
     8881 tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat
     8941 acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg
     9001 tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga
     9061 atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa
     9121 tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg
     9181 caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa
     9241 gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa
     9301 cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat
     9361 tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga
     9421 gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac
     9481 tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt
     9541 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9601 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9661 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9721 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9781 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9841 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
     9901 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
     9961 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10021 aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca
    10081 aaagaaatcg tgatcgatgc attagaagca gcattggttt ctgcatacaa acgtcactat
    10141 ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat
    10201 gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat
    10261 gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca
    10321 aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt
    10381 gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa
    10441 ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca
    10501 gttctttcta aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa
    10561 gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc
    10621 catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc
    10681 gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca
    10741 aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa
    10801 gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca
    10861 gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag
    10921 acaaattcaa aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt
    10981 aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa
    11041 tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa
    11101 ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca
    11161 ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag
    11221 atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa
    11281 aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga
    11341 aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg
    11401 gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa
    11461 cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag
    11521 ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca
    11581 cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg
    11641 caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag
    11701 ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca
    11761 aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt
    11821 aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat
    11881 ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg
    11941 gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga
    12001 aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac
    12061 gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc
    12121 gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata
    12181 cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta
    12241 ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta
    12301 atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac
    12361 caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag
    12421 cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc
    12481 ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga
    12541 cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat
    12601 tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga
    12661 ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg
    12721 taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg
    12781 atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg
    12841 cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca
    12901 tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg
    12961 ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg
    13021 agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga
    13081 aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag
    13141 atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata
    13201 aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc
    13261 caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc
    13321 gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc
    13381 aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag
    13441 aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca
    13501 acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag
    13561 atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg
    13621 tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag
    13681 cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag
    13741 tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg
    13801 aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg
    13861 gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct
    13921 atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg
    13981 taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca
    14041 aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg
    14101 taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc
    14161 aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa
    14221 tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt
    14281 gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc
    14341 atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga
    14401 gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc
    14461 agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa
    14521 aaatcccgca aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt
    14581 attacctaag agttccatga ctttagtctg gtagcgtgtc agctaaccaa aaaagagaaa
    14641 aagaaaagta aaaaattcga ctttcttttt tctatagtat ataaattttt gcatatagat
    14701 aaagatcaag ataccgataa cggaagctag attgtaatct attttagtga cagtcaggta
    14761 tagccaagta actagaagat cagtggaacc tgcaccataa aagtttgagt ttgtcggtcc
    14821 gttgacagtt aaaagaaaaa tcacgttgaa gttattgata tttccgatta cgccgcttcg
    14881 atttggtcac gatccaaatt ttggataatc cctgtagaaa ttagcatagt agctgggata
    14941 ccaattcaca tattgacaaa gataacagag acttttacat aaaacggatc aatcaggaaa
    15001 ggaattgctc gatccagccc cagttttcca acaatgtgtt aactggccta gcgtcattca
    15061 gcaaattatg catgatgagg agcgaaatga actgcggaac agccattgtg ataacgacaa
    15121 gttttttgga tcaatgtaaa aaacgtcagc gaaaggaaaa atttcgtggt tttttatacc
    15181 tgttcatttg gctagatata aaatccaaac aaatgaacag aagtgcttat taggtttcaa
    15241 ttgggagtat ctgcgctaga ataagagtaa taatgaaaag gagtgttatg tatggattat
    15301 tctagtaaag aatactttga taaaatgacg gcttggtggc gtgctgctaa ttatctttct
    15361 gttgggcaac tttatcttaa agataacccc ttactgagac gtacactgaa accagaagat
    15421 gtcaaaaaac atccaatcgg tcattgggga acgattcctg gccaaaattt tatttatgtc
    15481 catttgaacc gagtcatcaa taaatatgat ttgaacatgt tttatatcga agggccagga
    15541 cacggcggac aagtcatggt atcgaatgct tatttagatg gaagttacac agagatctat
    15601 ccagaagtaa cggaagatga gacaggaatg caaaaattat tcaagcgatt ttcatttcct
    15661 ggaggcattg cttctcatgc agcaccagaa acaccaggtt ctatccatga aggcggagag
    15721 cttggatact ctctttcaca tgcagtcgga gctgtattag acaatcctga agtaatctct
    15781 gctgtcgtga ttggtgatgg tgaagcagaa accggtcctt tagctggaag ctggttctca
    15841 aatgtcttta tcaatccagt gatagatgga gctgttttac cgatccttca tttaaatggt
    15901 gcaaaaatag ccaacccaac gatcttggca cgtaaatcag atggagaatt agcaaattac
    15961 tttaacggac ttggctggga gcctttcttt attgaaggaa atgatccaga aaaattaaat
    16021 cctgttatgg ctgaaaaaat ggatcaagca atcgagaaaa tcaaatccat ccaaaaagaa
    16081 gctcgcctga agacagcaac agatgtcgtg atgccaaaat ggccagtatt gatcgtacgc
    16141 acaccaaaag gatggaccgg tccaaaagaa tgggacgggg aaccaattga aggaaccttc
    16201 cgagcacacc aagtaccgat tcctgtagat caagaacata tggatcatgc agatgcttta
    16261 ctccgttggc tgaaatccta tgaaccagaa aaattatttg atgcacaagg acgtatttta
    16321 gaagaaatca gagaaattgc gcctactggt gaccaaagaa tggcaaaaaa tcctatcacc
    16381 aatggtggaa tcgatccaag accgcttatt atgccagatt ggaaaaaata tactttgcaa
    16441 tttgaaaaac taggctccgt taaagcagaa gatatgaccg aacttggtaa attcgttcgc
    16501 gagatcattg agaaaaatcc agaaaatttc cgtatatttg gaccagatga aacaaaatca
    16561 aaccgattga atcaagtttt caaaacgaca aatcgccaat ggatggaaaa aatcgaacca
    16621 gaaaatgatg aatggctttc gccatctggt cgcgtcatcg acagtcaatt atcggaacac
    16681 caagatgaag ggttcttaga agggtacgtt ctgactggac ggcatggttt ttttgcaagt
    16741 tacgaatcat tcttgcgagt ggttgactct atgttgactc agcacttcaa atggatacgg
    16801 aaatcacatg acctttcttg gcgaaatgat tatccttcgc taaatctcat tgcaagctca
    16861 acagtcttcc agcaagatca caatggatat agccaccaag atccaggaat attgacacat
    16921 ttggcagaaa agaaggcgga gttcatccgt gaatatctgc ctgcggatgc aaatacattg
    16981 ctcgcagtca tggataaagc attccactcg tcagaaaaaa tcaatttgat catctcttcc
    17041 aaacatccgc gtgctcaatt ttactcagca gaagaagcag ccgtcttagt gaatgaaggc
    17101 ttgaaaatca ttgattgggc ttcgacagca aaagaggagg aacctgaatt ggtcattgct
    17161 gctgcaggaa cggaatctaa cttagaagca ttagcagctg caactttgct gctggaagaa
    17221 ttcccaaaac taaaaattcg gttcattaat gtcgtagatt tattgaaatt acgtcatcca
    17281 agtcaagatc cgcgtgggtt gagcgatgaa gaatttgatc aatattttac aaaagacaaa
    17341 ccgatcttat ttgctttcca cggttatgaa acattggtcc gcacgatttt ctttgatcgt
    17401 cataaccatc atttaatgat tcatggctac aaagaaaatg gagatatcac gacaccattt
    17461 gacatgcgtg tagtaaatga acttgatcgt tatcatttgg caaaagacgc agctttaaaa
    17521 atcaaaggaa gtcaagcaga agattttgcg gaaaaaatgg atcagaaatt acaagaacat
    17581 caaaactata ttcgtgaaaa tggtattgat ctgccagaag tattggattg gaaatggaaa
    17641 aatcttgatc aataaaatga taaaatcgat cccgcaaaac agaatttttt acattgatta
    17701 agaaactgaa aaaataaata aaaaaaacgg aaaaaagact ggaagaatgg acagtctttt
    17761 ttttcgtttt tgggaagtga aaaccaaact atttacctgt aagccaccca taaatccaat
    17821 caaatggtgc aaagaaaaaa tcttccattc tattccctcc tttaaaaaaa cataacatga
    17881 tttctattta cgtgagcatg aattttgtta cgtttttatt gccgaaaaat aaaaaccgtt
    17941 cattatgatg tcaaattcgt cagtaattga tgcaatgtgt tataataatt cagttaaatc
    18001 aaaaatgaaa tggagaaaag acatggacgg gttattacct ttatggaaag aacgcggaat
    18061 gacaagtcat gattgcgtat tcaaattaag aaaaatctta catacgaaaa aaatcggaca
    18121 tgggggtacg cttgacccag acgtagacgg tgtgttgcct atttgtattg gaaaagccac
    18181 aaaagttatc gagtatttga cagattctgg gaaaatctat gtaggcgaga taactctagg
    18241 tttcagtaca acgacggaag atcgatccgg agaacgggta gaaacaaaac cagtagatat
    18301 gtctttatct gaagaaaaaa tagatgaagt gatggcttca tttattggtg agatcaccca
    18361 gataccacca atgtactcag cggtaaaagt caacggtaag cggttatatg aatatgcccg
    18421 caataatgaa acagtggagc gtcctgttag aaaggctcag atttaccgtt ttatccggac
    18481 aagtgaattg acaagagacg aagtgacagg aaccatttct tggcgcttcg aagtagaatg
    18541 cgggaaagga acctatgtcc gcacattagc tgtagataca ggcaagaaac ttggttaccc
    18601 tgcgcacatg tctgatctga cccgaacagc aagtgccggt atgtcccaga aagatgctct
    18661 gacattagaa gaagtagctc aagctgtgaa aaatgggaca atcgatgatt gtttgctgcc
    18721 aatagaaaaa ggagtgaaac gattcaaacg agtagatatt actgatgaag tatggcagaa
    18781 agtaaaaaac ggtatgagac ttgattatac tgtatttgcg ctcccagaaa tgccgtcaga
    18841 agaaattgct ttgttttata aaggcaaagt tgtcagtatc tatcagccaa acccgaaaga
    18901 aaaaaacaaa ttaaaaccta gtaaagtatt gagaaatgag gaataacgaa tgaatatcat
    18961 caagatcaga catccttacc aatcagaaca aattccagaa gacgatgtcg ttttagtact
    19021 gggatttttt gacggcgtgc atattggaca ccagaaagtc ataaagacag gtatggaaat
    19081 tgcacaaaaa gaaggattga aattagcatt gatgactttc aatcagcatc catcgattgt
    19141 ctttaaaaag atcgatccta gcagcgtgaa atatttgact agccttaagc aaaaagaaga
    19201 aaaaatggcc gctttgggta tagattattt atatgaagtt gattttactt cttcttttgc
    19261 tcatttagca ccgcaggaat ttgttgatca atacatcgta gggttacatg caaaatatgc
    19321 tgtttccggt tttgattata cgtatggacc aaaagacatt gcagatgtcg ctcattttcc
    19381 tgaatatgcc aaaggaagat tcaaaatcgt cacagtttct aaagaagaag aacaaggtga
    19441 aaaaatcagc tctacccgta ttcgaggatt actagacagc ggacaagtaa aagaagcagc
    19501 aaaactatta ggttgcacat atgaggtaga aggggtagtt gtccatggcg atgcacgagg
    19561 cagactacta ggattcccta cagcaaatgt caaagtaaag agcacagttc atttgcctaa
    19621 agaaggtgtt tatgtatcgg aaataaaaat tggggaaact tggtatccag ccatgggttc
    19681 aattggacat aatgatacat tcggtgaagg acgggagctg actgtagaat tgtatatttt
    19741 ggactttaat caagatatct atggtgaaca tgtcgcagta cgctggaatc aatttttaag
    19801 agatcaagtg aaattttcta gtgtagagga attgattgaa caactcaaaa aggatgaaca
    19861 agcaacagct gaatattttt ctcagcaata aaacattatg cataaaatct cttgtttatg
    19921 tataactagc aatttcaata aaatgggtga taaataagcc caggaaaggg gctgtgacaa
    19981 aagtcttgtc acagc
//
LOCUS       DAFEOM010000045        19785 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.046, whole genome shotgun sequence.
ACCESSION   DAFEOM010000045
VERSION     DAFEOM010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.046"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            <1..1868
                     /locus_tag="LP524_001828"
     CDS             <1..1868
                     /locus_tag="LP524_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBL2234866.1"
                     /translation="TPPEKVNITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTK
                     YNKADYGDVEFTLGNITDQVLPTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKIT
                     SAVDENGEITFADQPAYVNSKGNVYVIYESKSAAGLVTQKAKPMVVIAPMTDNTGSGF
                     LKDIHLYPKNIVSKLSFELTKFGDDGTEQSKQTPLKGAKFELYKGEPGKGSKLGDLVS
                     DDQGKLTATDLTLGKYYFVEVPSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITN
                     DGVTSDLKASYVNYKAPVIDKTVTNGTGQEHSFQVGDAVNYQGTIHIPTDIAGGADGI
                     TVNGVKSETSPYSVFKWGDTAGQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSEN
                     GFVIDFIVNNGQVSDTVASLHGQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQ
                     EEHHEKTKADVVTYGAKFLKVDSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDK
                     DGVKEAVWVDDVANAAILKSDKEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKV
                     DKDSYKEENRITIKNNQKASVPMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            1881..2633
                     /locus_tag="LP524_001829"
     CDS             1881..2633
                     /locus_tag="LP524_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL2234867.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVSKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            2649..3404
                     /locus_tag="LP524_001830"
     CDS             2649..3404
                     /locus_tag="LP524_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234868.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADSQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGHEKQEGVNG
                     AKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLVSNGETKTIDQQTGV
                     FEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSEKNGEFLKDVWIYPKSEAS
                     QPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            3417..3677
                     /locus_tag="LP524_001831"
     CDS             3417..3677
                     /locus_tag="LP524_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234869.1"
                     /translation="MKLIKLLLFNLSCLTAVFLYCGLLFSQLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            3674..5764
                     /locus_tag="LP524_001832"
     CDS             3674..5764
                     /locus_tag="LP524_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBL2234870.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRQDAVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVDYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            5791..6255
                     /locus_tag="LP524_001833"
     CDS             5791..6255
                     /locus_tag="LP524_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234871.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            6252..6494
                     /locus_tag="LP524_001834"
     CDS             6252..6494
                     /locus_tag="LP524_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HBL2234872.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            6512..6814
                     /locus_tag="LP524_001835"
     CDS             6512..6814
                     /locus_tag="LP524_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234873.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            6884..8947
                     /locus_tag="LP524_001836"
     CDS             6884..8947
                     /locus_tag="LP524_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234874.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKYEKIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEE
                     AQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            8947..11556
                     /locus_tag="LP524_001837"
     CDS             8947..11556
                     /locus_tag="LP524_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBL2234875.1"
                     /translation="MRVRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGGFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYQDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNIL
                     STAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHQGKK
                     FHEERVKVGYRGFCEINFDCHLEQGDHVCLKYDHRGKEYVAWYEFSQRVLKDEEGNIV
                     YRKKRGETHLPELEKEAVLTLDEDKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHII
                     VSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNMLW
                     DLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENST
                     INSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNHG
                     ETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDIYARRLYHNMH
                     DQIEEEAKENIFIKIVQSLDNSTAQKLLIQFYEEKQIDRLQEYLEKLRQNEEITEVIR
                     KRLLLEAEKAVKE"
     gene            11553..13925
                     /locus_tag="LP524_001838"
     CDS             11553..13925
                     /locus_tag="LP524_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234876.1"
                     /translation="MIIGGIFSGIADWWSGLFGDGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKIKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLENDPNKVEHIPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNVAKNVGSGAKNLASAGKSGLSNLNDIRKNGFGN
                     RKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTAE
                     DLTEKAKDTLEEKLDGTRAKELAEAVKNPSETLEKQKEKASEIKEGLKDAYGEGQVNA
                     AVKANTTTEKENQEGAAIPSAKDHQDELHSNENSEEGQNGQLPKTDPEQYRYKSNINS
                     ENEKRISDLPKRNQEVQTDDDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMKDSRLNQ
                     FKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLSESGETENASVQNIVTREAEP
                     KPIQNVHTGYKPANVAFTAPTKINVTRDTKNRNNALKEQLLRDIKQDIKP"
     gene            13975..14307
                     /locus_tag="LP524_001839"
     CDS             13975..14307
                     /locus_tag="LP524_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234877.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            14308..14901
                     /locus_tag="LP524_001840"
     CDS             14308..14901
                     /locus_tag="LP524_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234878.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLVTKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            15064..16758
                     /locus_tag="LP524_001841"
     CDS             15064..16758
                     /locus_tag="LP524_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBL2234879.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLLKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            17446..19323
                     /gene="ltrA"
                     /locus_tag="LP524_001842"
     CDS             17446..19323
                     /gene="ltrA"
                     /locus_tag="LP524_001842"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBL2234880.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            19454..19785
                     /locus_tag="LP524_001843"
                     /pseudo
     CDS             19454..19785
                     /locus_tag="LP524_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
BASE COUNT         7297 a         3171 c         4065 g         5252 t
ORIGIN      
        1 aaacgccacc agagaaggtc aatatcacgg ttcataaatt aatgtacgat caaagtacgc
       61 aattgaatgt tgatattgac gggatcaaaa atgatggtta tacgcacgat cagtatccta
      121 caggtgtaac gaaatacaac aaggcagatt atggtgatgt tgagtttacg ttaggaaata
      181 ttacggatca agtattaccg actgaagatt ctgatctaac caacgcgaaa gtggacgaaa
      241 tcgtgaaaga cgtagaagac aagggatcta acagtgagta cgtcaagaat gcaaccaata
      301 aaattacttc agctgtagat gaaaatggtg agattacttt tgcggatcaa ccagcgtacg
      361 tgaactcaaa aggaaatgtg tatgttattt acgagagtaa gtccgcagca ggattagtaa
      421 ctcaaaaagc aaaaccaatg gtggtcattg ctccaatgac tgacaacaca ggttcaggtt
      481 ttttaaaaga cattcactta tatcctaaaa atattgtttc taagttgagt tttgaattaa
      541 ccaaattcgg tgatgatggt acagagcaaa gcaaacaaac ccctcttaaa ggcgctaaat
      601 tcgaattgta taaaggagaa cctggaaaag gaagcaaact aggcgatctt gtttcggatg
      661 atcaaggaaa gctgactgct actgatttga cgttaggaaa atactatttt gtggaagttc
      721 cttcagaagt cgtagtggga tcagataaag aaccaacagc cgatcaatat ttattaggcg
      781 cagacgctcg caacgatgcg cacaataaat taacctttga aattacgaat gatggcgtga
      841 caagtgactt aaaagcttca tacgtaaact ataaagcacc agtgatcgat aaaacagtaa
      901 caaatggaac aggacaagaa cattctttcc aagttggtga tgccgttaat tatcaaggaa
      961 cgattcatat cccaacagac attgcaggcg gagcagacgg tatcacagta aacggcgtga
     1021 aaagcgaaac ttctccttac agtgtattta aatgggggga tacagcagga caaggacttt
     1081 cttatgtcgc tgctaaagca aatatcaaag taacaaataa agatggatca gtggtattaa
     1141 aagaaaatac agattataag atccaaaatt cagaaaatgg ctttgtgatc gattttatcg
     1201 taaataatgg ccaagtaagt gatactgtag caagtttaca cggacaagat cttcaaatga
     1261 cttataacat gtatgtaaat gacagcgcag ctgttgccaa tccattaaca aatagcgtgg
     1321 atttcgttta taacaacaat ccatttaatc aggaagaaca ccacgaaaaa acgaaagctg
     1381 acgttgtgac ttatggcgcg aaattcttaa aagtcgatag cggactattt ggaacaggaa
     1441 ttaaggcaac tcctcttgaa ggcgcagaat ttgcagctaa gaatgcagaa ggtaagtact
     1501 acggtggact tactgataca gataaagatg gtgtcaaaga agcagtatgg gtagacgatg
     1561 tagcaaatgc agcaatccta aaatcagata aagagggtca ttttgaaatc actggtttaa
     1621 ctgaaggaga ttactcactg gaagaaacaa aagctcctga aaactatcaa aaattaacaa
     1681 aagagatctc tttcaaagta gacaaagatt catacaaaga agaaaaccga atcacgatca
     1741 aaaataatca aaaagcttcc gtaccaatga ctggtagcaa cggtttccaa tcttacgtat
     1801 tgatctcttg cttgttgctt ggggcaggcg caatttctgc agttgtttac tttaagaaga
     1861 aagcctagaa ggatcagcta atgattatca gacaccctaa gaaaaagcga atcatgggta
     1921 aatggatcat tgctttctgg ttactttcag ctgttggtgt cttacttttg atgcctgctg
     1981 aagcgagcgt ggcgaaatac caacaaaatc agcagattgc cgcaatcgat cgaacaggaa
     2041 cggcagcaga aacggattct tctttggatg tttctaagat tgagttaggc gatcctgttg
     2101 gtattcttac aattccaagt atttctttaa aactaccaat ttatgacgga acttccgaca
     2161 aaattttaga gaatggtgta gggattacgg aaggaactgg cgatattaca ggaggaaacg
     2221 gtaaaaatcc tttaatcgca gggcacagtg gactttataa agataatctt ttcgatgatt
     2281 tgccttcagt taaaaaggga gaaaagtttt atatcaaagt tgatggtgag cagcacgctt
     2341 atcaaatcga tcggattgag gaagtgcaaa aagacgaact acaaagaaac tttgtcactt
     2401 acttagaacc aaatcccaat gaagatcgag taacgctaat gacatgtacg cctaagggga
     2461 tcaacacaca ccgtttccta gtgtatggga agcgagtaac ctttacaaaa tcagaattaa
     2521 aagatgaaga aaataagaaa caaaaattat cttggaaatg gcttttagga agtactgttt
     2581 ttctttcagt catgatcatt ggttcgctat ttgtttataa aaagaaaaaa taaaggaggg
     2641 taaccaacat gagaaataga aaatggctat ttgtttgtgc agccgctctt ttttctttca
     2701 ctgcctacac acagttgacg tttgcagaca gtcaagaaag cacggatagc acagaacaaa
     2761 caatccaaac gtcagatagc agcacgattc aaagcagcga tgaaaccgtc gattcgtcaa
     2821 cagtggattc ttcagaagaa ccgaaaattg ctgaagacga tcaaatagtc aaacagacca
     2881 ttcacgttca aaagattatt tcttcagaac aattagacaa ccaggggcat gaaaaacaag
     2941 aaggagtaaa tggcgcgaag ttttctgttt atgatgtatc ggacatttta caaaagatgg
     3001 atgttaagga tctcactact gaccaaattg aatcccaatt aaaagatcga gtgaaaaaac
     3061 tatccagtga tcaattgaag ctagtttcta atggagaaac aaagacaatt gatcaacaaa
     3121 caggtgtctt tgaattctcg gtagaagtac aggcaaatca gaaacaagct tattatattg
     3181 tcaatgaatc aagtcctgaa aacatttcta atagcgagga tattttgcta ttaacacctg
     3241 tttctgaaaa aaacggtgaa ttcttaaagg atgtgtggat ttatcctaaa tcagaagctt
     3301 cgcaacctaa agaagaagta aaaaaaatcg tttctactgg tgtaaagaaa aacttctttg
     3361 agaattgttg ggattttgtt actcacttgt tttctagaga ttaaggaaga aataagatga
     3421 aactgatcaa attattatta ttcaatcttt cttgtttaac cgcagtcttt ttgtactgcg
     3481 gtttattatt ttctcaatta tctattttgc caagctacca actataccta atgaggattt
     3541 taaacatacc agctgcttcc atgccgctag atattatgga aaagctcatt aggaatccat
     3601 tatttactac gatcaatttg atcgttattg tcggaatttc actagtcatt tataaaaaag
     3661 aggggatgaa gggatgaatt ttaaacactc gtggaccaga cggttaaaat atgtttttcc
     3721 cataatgatg atgttaggta cattgtttgg gttaaaaaca gccaatgtct ttgctgaaaa
     3781 agttattgtc gatccgtcaa atccaatcca gaacgtaaag aataatttta ttattccaaa
     3841 agatgttcct aatgcaaaaa caaatattac tgtaaacgga gcgtccaaag tttacaaata
     3901 tcatgttgat gccaacaaag gtggttttac agacgactac gtaggattag tggagggaaa
     3961 caaaaatata cgctatcctt ctttccaaaa ggaatacggt gaaacaaacc ttgttttaga
     4021 aggcgtaaca accaacacca aagtcaatat tgactatgga aaaatcggta cgtataacgg
     4081 gaaaaaagtc aatatcaagc tagttctatc taatattcac ttatactcgg atacgttgcc
     4141 gtggaatcta ctagataaca actatacaaa aactcatttt cgagacgatg gctataaaaa
     4201 tacgaatggt gcaatgagta aatctaaaaa aagaacagta ctttggatct cagacaatct
     4261 tttctcagga attgtttacc attcaacaca aatgaatgtg cagctagtag ccacatatga
     4321 ggacggtagc cccgttcaat tttcaggaga tacatttatc agttttaact ctttgaaccc
     4381 tgcaggagga aaaagcacgg atcttaaagg agaatatgcc cactatgaca aaatgaatac
     4441 gacagattgg tatgtcagac aagatgcggt tctttcagaa tttaaatctt tctacaacaa
     4501 tctgaacgtg gtaggtggcc acccaggagg aagctctaag ctgacacaag cggataatga
     4561 tttcaataat ttgcatgata aattaggaga tccgaagttt ggtcaaggta ctgtatcctt
     4621 taaaatatct gaagcaaatc cgacgtttgt gattggttct tctaatgttc aaacctggtt
     4681 tactttatct tctgcgacaa tctttagtgt tgttcctgat caacctgaaa aaacaggggt
     4741 tgataaaaat ggaaataacg tgaacgataa aatgttgcaa gtaggagaca cgattcaata
     4801 tcgaatcaaa cagaaggtga atcgattagg agtggattta ctagcggttt acgatcgatt
     4861 tgagttaatc gataatttac caaaagaagt agattacgtc gatgcacacg ttgaaagtgg
     4921 tacaaataaa aaattcgatg tatctggtga agtgacctat gacaaaacaa aacaccaggt
     4981 aaaatatgca gcgaaagctg atacattaaa aaatcgaatg aagtataacg gcgaaactta
     5041 cgaattagtg atcaatgtaa aagtcaatga attggccaat caaaatagtg tagccaaaaa
     5101 tcagggaacg tcgatcatta ataaagtaga aaaggataca aatatcatta ccgtttattt
     5161 ccctaaaatt ccagtgaaag aagtacaaca aaatggaaaa gatgtaaacg gacgaaatga
     5221 cggtaagaag ggtacgccga cagctccttt aaacgcagga tctgaagtac aatatctagt
     5281 cacacaaaaa tggcacacaa aaggcgtaga cgctgcgtca gatcactaca aacaatttag
     5341 tattcaagat ccaattgaag ctcgtttgac atataaagaa ggttctgcgc aagtaataga
     5401 taagagtaaa ggaaaagata ttacttctga aggcacacta acctatgata gcaattcaag
     5461 aacgctgaag tgggaagctt ccgctgactt cttaagtaac aatcttttag atggacgaga
     5521 aattcagctg atatttacag ctaaaactcc attacaatca gaaaaaaata ttgataacca
     5581 agccgtagta gcagtagata atgtttctaa taaaacaaat gttgtgacaa ttggggttga
     5641 tcctaacttg ccacaggtca ttgttcctaa aacaggttct acacatttag taacgatttc
     5701 agcggtcagc ttactattac ttgtgttagc aacttttagt tatgcggttc tgagatacat
     5761 ttaaaataaa aaaacgaaag aatgaataaa atgaaaaagt taataatagt agttacttcc
     5821 ttgattgcca tgattgttat tttagtcgga tgtagtaatg aaaaaaataa acaaactaat
     5881 caagataatg gcgtattgaa atcaggaact atgtggaaag aagaagtagg tggtcttgtt
     5941 tataatttga aaattataga cgagactacc tgggaatact ctgagagtgt atggcatcca
     6001 gatcctgtac aaataacagt caagaggcaa aaagactata aaggtttaga aagatacaaa
     6061 atagtagatt cagctggtgt tagggaattt ataaacaaat ctgattcttt atttattgtt
     6121 gttccctatg agaagaatgg cgtaaaaaaa ataatttttc ttggatctag caaggatgaa
     6181 aagcgaacga aagagaaatt gattcacgat ggatcacaat ccaataaata taagttacaa
     6241 aagactagcg agtgattgct agtcttttta tttgtcctga aggaggaatc acagtggcaa
     6301 aagctaaaac gccatttgaa aaagccgcgt ctaaaatctt agaacgccgc ggtgtatctg
     6361 aaacacagtg gaaacaagaa gtccttgaaa aagcacaatt tgacctatta cggggaaaag
     6421 acaaggactt agaaggatat atctatcaac gtgaacgtga aaagcttgtt ttcgatgaaa
     6481 ttattaaaca ctaaaaggag cgaacaattt tatgtttgaa tcaattacaa tgaatttttt
     6541 aacacaaccg aaagtagtag ctgcggttga tatttttaga gcattacaaa ctgccgcaac
     6601 tagtttaaaa aacaatctga tgatttttgc tgctgctgcg gccgttgctt ccattgttat
     6661 tgggggtctt ttcttcctat ttggacgaag aggagctgaa aatgggaaga tgcacgtagg
     6721 ctatgtattg atgggggtag gagttatttt agcagcagct tctattgtgg tttcgttatt
     6781 tggattgttt ggacaaacac ctcaaggatt ataagaaagt aaaaaagtag gagtaagaag
     6841 tagtcgatac tttttactcc ttttttatga aaggagaata ctcatgtact ttacgattgc
     6901 aggcaatacc aaaacagata aagagttaat aaaaaaagca acgaaggaag taggaaagcg
     6961 tacttctttt ttaccgaaaa caattaaata ttctgtcatt gaaacggaag aacagctaca
     7021 tgtgttaggg aaagtactgg aacacgaaag tcgatcttct gttaagttga ctctcacgtt
     7081 acatgacaca gaaggaaaaa tagagaaagc tggtacagaa tacgtttttg gcccactgga
     7141 ttatcaaatt tcagaaggtt tctatcctac attgatggat ttaatccgcc ttgatgtgtt
     7201 taaagaaacg gatgaaacta tgagtgtgga agagtcttat cgaaacgaaa aacttttaca
     7261 agttttagaa gaagagttag agagcgacca aagaaaagtg aatcaaggga gtgaagtcga
     7321 agtaaatgaa gaagtaccat gggaacgacc aatatttcca aagaaggagt caaaagaaaa
     7381 agagacagtc tatgttgacg aacaagctga tattccgact cagaaatcga tatttgagga
     7441 agagaacgat gtgatgggtc aattggaggt agatcccttt gaagaaacaa tggaggatga
     7501 agagacggcc gaagtagaag ttgatccagt tgaagcgaat gaagagtcaa catggactga
     7561 aggggattct caggaatata ttgaaccact tatcagtgaa gaagtagaaa aaaacgaaat
     7621 agagatacaa aaggcaactg tatttgatta tattttggat caatatccgc cagagcatga
     7681 atggatgaaa gaaaaaaaca aattattttt aaatgagctt tattcgaaat atcaattacc
     7741 ccaaacctat caagcatttt tagagagtaa agaagaatta atctcacagg gtaaaggcac
     7801 acttttagaa cagttagagt ctgtccaatc aacaaattgg catgagcttg cacaagaaga
     7861 tttattagcc acctttgaag aacgtcaaca agaaacagat caagaaatta atgagtatgc
     7921 gacggaacag tcgtctaatt gggagaaagc aaaagaggaa ctaaataaag aagaacagcg
     7981 agtgattgaa gaagaagtac aaaaaattaa agagcgatac gaaaagaaac gcgacaatgc
     8041 gtattccttg tgtgtagcaa agatcaataa ttttactcag acgaatcaag aagcgttatc
     8101 gaaagaaaaa gaaaacttgt taaaagaacg tattaaagaa cgaaaagaag aattttaccg
     8161 aggtttgcgt acagataagc taaagattag caaagaatta aatgaacaac tgaataagct
     8221 atatcaaaca acgactgaag ttcttatgga aaaatacgaa aagatccagc aagaattaca
     8281 agtgcaaatt ccaaaatgga aaaaagagta tgaaattcaa caacaagaat tgaaagaaca
     8341 agaagaaaaa caacggcaaa aagaaaaaga gcgtgaacaa ttagaaataa gaaaacggga
     8401 acaggatctt gcggaacaaa aaatggcgat agaaaaacaa cggttagagc aagaaaagga
     8461 aaaagaagca gctgctcgta aggaacgtca agaacaaatg gatacattgc gacaagctca
     8521 gttaaattat ctagcacaac ctcaaccaac gccttcagta gctgctgttc aaaccccaat
     8581 gaacaaccaa acaaatctaa ctccttcacc acagcgttcc tttaacggat ggatggtggg
     8641 atgtattgca agtttaagct tgcttctagg tggaggaaca gtatttgctt ttaatcatat
     8701 caatcaatct tcacaggctg attctgtggc cacaacgacg tcaatcaagg aagaagccca
     8761 agaacaagca agaaaagagt atgaacaaca attagaagaa ttattgaatc gtaccaatca
     8821 gtcaacaaca gaatcaagta tggaaaatag caaagagagt gatcctagta aggctaaaga
     8881 aggatcaagc gattcaactg tacaatcaaa cacttccgaa agttcagaat caaatgaggt
     8941 gaaataatgc gtgttagaga aacaacgaga cgaaaagaca aacgattcaa tttagaaaag
     9001 aataggtatc tttctcattt ctatcgctat actccttcag gagaagagct accagaaaaa
     9061 catgacaaac gtatttctca aaagattttt tatagtggct tagcttatct tcctttaaac
     9121 tatgttgtta caagaatacc agaagcattt catcgatttg agattattcc aggaagttta
     9181 caaggagtga atatttttca gttacaagaa atgccgcagt tattccattt tgattcaact
     9241 ggtttttgga atcctttgac cccaacaaca agtttaggca cacgtttagt tgtttctacg
     9301 atttgtgttg ttccagctgt acttatggga ttgaaaatgg acatgtggtt tcgtcctcta
     9361 gcagatgggc aaaaaggaga taaccgatta aaaacgttaa aggaaatcca acaaatttat
     9421 ccgcaaatac cagaatcaaa gggaggtttt cctggttatg gcggaatacc aattactcat
     9481 tataaaaagt actggtatat tcagacggat acagtaaata cttgtattgt tggtacttcc
     9541 cgttcaggga aaggacagat agaagtttta gcaaccattg acaatttgtc tcgtgcagaa
     9601 agacaaagtt caatggttgt taatgatcca aaaacagaat tatatgttgc tagtaaagat
     9661 gaactagaag ctagagggta tgatgtgtat gcatttaata tcaccgatcc actgcaatca
     9721 atgagtgata atccattggc actcatcgtg aaatactgga agcgaggaga catagacaca
     9781 gcgacacagt taaccaatac tttcacgaat acgatttatt atgatgccaa cgcttcagac
     9841 aataaatact ttaacgaaaa tgcgcaaaaa gctgtcaatg cgcttatttt tgcgttattg
     9901 gaacaagcgg atcagacagg ggattactcc aaattaacac cgaataatct cgtcgaactt
     9961 ctttctgaga ttggtggttt taattaccaa gatcctgata atgaattcaa gcagctcaat
    10021 gctttagatg aatttatgaa tcagttacca cctggaaaca tagcaaaaaa acaatatggg
    10081 gcaacaaaaa ttggttctga taaagcgaaa ggcaacattc tatcgacagc gatcacagga
    10141 ctaaatccat ttacactgac gaaaattgcg aaaatgactt ctcaatcaac cattgattat
    10201 aagtccattg gttttccgaa gtatttggat ctcaaactac acgagagtct gttaaaccag
    10261 cgaattgttg tagagtttta tcatcaagga aaaaaatttc atgaggaacg tgtcaaagta
    10321 ggctatagag gtttttgtga aattaacttt gattgtcatt tagaacaggg agatcatgtt
    10381 tgcttaaaat acgatcatcg aggaaaagaa tatgttgctt ggtatgagtt ttctcaaaga
    10441 gtattgaaag acgaagaggg aaatatcgtt tatcgaaaaa aacgaggaga aacccatttg
    10501 ccagaattag aaaaagaagc cgttctaaca ttagatgagg ataaaagtaa cttattagta
    10561 gagtctattc ggatgcacta taccgataaa ccaatggcta tttttatgct cacacctgat
    10621 tatgatccat ctaaccatat cattgttagt atttttctta gtcagttgta taaagaatta
    10681 tctacgcaat gtgttaagac taaaggagat aaatgtcacc gtaggattca ttttatttta
    10741 gatgaatttg ggaatatgcc accaatggat aatatggaag gaatcatgac agtcacagct
    10801 ggtagaaaca tgttgtggga tttgtttatc caatcctatc agcaacttta tgcgaagtat
    10861 ggggaacaaa acgcagcgat tatcaaagaa aattgtcaga cacatattta tattatgtcg
    10921 acagacgata ataccataga agaattttcc aaaaaagtgg ggaataagac ggttgaacaa
    10981 gagaacagta ccatcaattc attaggtatg aatactcata tcaatcgcaa tgttgattca
    11041 gatcgcatct taactcctga aagaatttct acttttttag aaggtgaaac gattgtactc
    11101 gctcctttaa aacgacaaga tttgaaaggt cataaaatac ggccgtatcc tatttttaat
    11161 catggggaaa cgaatcttcc ttatgcttat caatatattg aagatttcaa tccatcgtgg
    11221 gatatcaatg atatagacat acattgtaca catgcggact tggatcttaa ggctttacaa
    11281 attgattacg attattttct acctgatatc tatgcacgtc gactttatca caatatgcac
    11341 gatcaaatag aagaggaagc taaagaaaac atttttatta aaatcgttca atctttagac
    11401 aactcaacgg cacaaaagtt acttattcaa ttttatgaag agaagcaaat agatcgtttg
    11461 caagaatacc ttgaaaaatt gcgtcaaaat gaagaaatta cagaggttat aaggaaacga
    11521 cttttgctag aagctgaaaa ggcggtgaaa gaatgatcat tggaggaata ttttccggca
    11581 ttgccgactg gtggtcaggc ttatttgggg atggggatgc agatcccaaa accgtaacga
    11641 aagtcttaca agattatggt aattatctgc agtatcaaga tgtattcagt tggatttgta
    11701 atactttttt atggggtttg attaaagggt tatataatct caatacttta ctagaaaaaa
    11761 caatttatca aagctttgat ttaaaagact tattgaacgc cgctggtgtt aatgaactgt
    11821 ttcaaagttt gattagtaag gtattggctc ttttttgtgt cgtcacatta atttatttag
    11881 gcttaaaatt ttttatgagt aaacatccac caaaaatcaa aaatatcttg gttaatctat
    11941 ttatggccat gattttgatt tttggtggta gttctttaat cgatgaaggg ctaaatatta
    12001 gtaaaaactt ttacggagac gtgactgcag caaataaagc agataaaact ggttcacctg
    12061 cttttcaaat tattcaaaat aatgtgattg atgtgagtac gttactagaa aatgatccga
    12121 acaaagtaga acacatacca acagacaaaa gaaatgctct cacggcgaaa aattttaaaa
    12181 cagcgaatat caatgggatt attacacctg atcaagctaa gaaaatgtct gaatcaagtg
    12241 atattagtac agtacaaaaa gatcggttaa aggcattaca gtatcgacta gagttagacg
    12301 ataatgggaa agaaatccct gttcaaatta gtgatgaagg tttagcaaaa tatgtgtata
    12361 cttcaggcta tcgccgatac attagtagtc caggtattat tttagctggt gaatcttctt
    12421 tgtcggtagc ctatttattt atcttgttta cgattgttac ttgcattatt gagctggtat
    12481 ttaaaaaatt ctatttagtg attgccgcgt caacggactt tgagacagga caacggatga
    12541 aaagtgctat agaagatatt tctcaatcct tcttgttatt ggcatttacg attttagaat
    12601 tacgaatcta tacattaatg ctttcaggta tcggtgattt gcatgcggca ggaaagatca
    12661 atggattctt gtatgttgtc gctttaattg tattaaccat tgcgctattc aaaggctctc
    12721 aatcagttac gaaaattttc ggcgttgata cgagtctgaa aaacggcagc aattctcttc
    12781 ttagcatgtt tgccttagga aatgttgcga aaaatgtggg tagcggggct aaaaaccttg
    12841 caagtgctgg taagagtggt ttaagcaatt taaatgatat tcgtaaaaat ggatttggta
    12901 atcgaaaaga tgttacccct gataacgggg aagaacaatc agacgataaa gctacaactg
    12961 tacctaatag taagaaaaca gtttccgaaa tgttcgggaa agcgaaaaat ggtgtttcta
    13021 aagccgcaga aggtcttggt tacgtcaatg aacgtgggtt aaaaggaaca gctgaagatc
    13081 ttacagaaaa agccaaagac accttagaag agaaattaga cggcacgaga gctaaagaat
    13141 tggctgaagc tgtgaagaat ccatccgaaa cactagaaaa acaaaaagaa aaagcttcag
    13201 aaatcaaaga aggactaaaa gatgcgtatg gtgaaggaca agtcaatgca gcagtaaaag
    13261 ctaatacaac aaccgaaaaa gaaaatcaag aaggtgcagc gattccttca gccaaagatc
    13321 accaagatga gttgcattct aacgaaaatt cagaagaagg acaaaatggt caattaccaa
    13381 aaacagatcc agaacaatat cgttataaat ctaacattaa ttctgaaaat gaaaagcgca
    13441 tttcagattt accaaaacga aatcaagaag tacaaacgga tgatgattcc atcgatctgc
    13501 caaatgataa tggcatcgaa gttggaactg aaaaatcaac gaaaattggt ttgcctaaga
    13561 cacaaaattt accaatgaaa gatagtcgtt tgaatcaatt caaagagaat gatcaaagct
    13621 ttgttcatca atcacctgca ggtactgttc aaatgaaatc aagtcgtttg cctgaaaatg
    13681 ggaatttgac aactgtttct tacaatttat ctgaaagtgg tgagacagag aatgcgtccg
    13741 ttcaaaatat tgtgacaaga gaagctgaac caaaacctat tcaaaacgtt catacaggtt
    13801 acaagccagc aaatgtggca tttactgcac cgacaaaaat aaatgtgaca agagatacca
    13861 aaaacaggaa caatgcattg aaagaacaac tattgaggga tatcaaacag gatatcaaac
    13921 cataaaaggc ctacaagtta ggccttttct ttatatgaaa aggagaaatc aattatggat
    13981 aaatatataa acatgaaaaa ccctccaaga tttagtggag aatggaagtt tttcgctttt
    14041 ttctttttag atctattaac gcttggtggc tgcttttttt gggcgaaaat ggtcaataat
    14101 cttgtccact taccactctt ttttgcactc ttgaattatg gcttatggat tgcgatagcg
    14161 ttagtgattg tttggcgacc ctataataac ccaggtcaac gacaaatttt tgcgattatc
    14221 gagtctattt tttttgcgga caagaaccat tatcatgcgt tagatcctaa ccgaaattat
    14281 catggtacgt ataaggagga tgaataaatg cgagtcaaag gacaaaattc agttgataag
    14341 aaatctattt tgcagtttgg aaaaccagaa gaacgatcta tcttggattt atttccttat
    14401 aaatcctgta cagaagaggg agtcttagtt actaaagaag atcattttca acgttattat
    14461 cgtgtcgttt ccactgacgt ggagggatta aatgaacaag agaaactgga acgaatgaat
    14521 cagttgacca ccgtcatgcg tacgtatgtg ccaacaatta agttagttag tttgactaca
    14581 gagacggatc taacggaaca aatcgtgcaa aagcggcagt tattagataa gaatcgtatg
    14641 gaacaatcct taggaagaaa tctacaacga ttaaaaaaat atgaaaagaa attagtcgaa
    14701 gaaatccagg aattgaaacg agcagaacaa gaacgtcctg acttaagctt ctttttcgtc
    14761 attgaaggaa agacaatcaa ggatttacaa accagaaacc gtcagttaat gcgatctagt
    14821 ggcattttag ggttaaaacc actctacaaa aaagaattga tcaagattct gtatcgaatg
    14881 aacaatatga atgatgagta ggaatggaga agagagaatg aaatatccat tgatatggtt
    14941 agaaaaagat gttccgaagg aagagaccac tagaaaattt cctcacactt taagcctggg
    15001 taagttggga aaagggcaat cttttaacca agtaaagaat caaacaagaa gaggtgactg
    15061 tacatgttag aagcaattaa aacagctttt tcaccacaaa atgatcaaaa agtacttgat
    15121 ccattaaagc aactaggaaa tcgggaaata gaaattttga aatccaaagg gtatgattta
    15181 gattttttaa aacaagtgat gccacaaggt gggttaaatt ttgatgaaga atatgtgacg
    15241 tatggtaatg gttattcacg atgtgtgaca gtttatcact acacaaaaaa tcctacgctt
    15301 ttttggttgg ccaatctcat gaacaatcct catacagtgg ccacactcga tgttagcaca
    15361 gatgaaaagc aaaaagtgat tgatcaaatc aatcgtgcct tagatgaatt aaatgatcga
    15421 tcagataatg aacgaaaagc gactgaccga aataaaagcg cagaagaata cctggaacta
    15481 gcggaatatg ctcgtaaatt gacttctaat ggggaaattt ctaagcaagt caaagtgaga
    15541 gtacatgttt acggaaacac gattcaagaa gtagatgaac gagcgcagga actgattgaa
    15601 aatttaaaag ggatggatta tcgtgcagtg acctatctgc acaaaacgaa aagagattgg
    15661 caatcgatgt ttaccatgtt cgatgaacaa gctgaatggt ttggaaatat caaagggcaa
    15721 tcactaccat cgttaaatat tggcggagga ttcccgttcc accaccaaac gctaaaagat
    15781 ccaggaggga ctcaatatgg ggaaaccatg acaggtggcc catttgtctg gaatgcgaca
    15841 tataaaaata gtgtgcggtt gtctactaat accttgcttc taggaatgat gggagctggt
    15901 aaatcaaccg ttcttaaaat gatcgcggaa gcccatttag ctgcaggaga ctttatttgg
    15961 ggctttgaaa aggggaaaga ctttatcccc ttcttaaagg aatataacgg cattatggtg
    16021 cgcttagacg ggtctgacgg gatgatcaat cctttagaaa tatttgccac acggacgtat
    16081 gatgaagcat ccagtttata tgacgatgga tcagtcaaag atttgaagat caatgaagca
    16141 gcgtcctatc aaacacactt agacaaagta gtctatcagg tacagttagt gagtcctcag
    16201 ctaaaaggaa cgatgaaagc agaatttaag acctacttaa atcgttttta tgaagagtat
    16261 ggaacagtac cacgcggctt tacttcctca aattcgcgtt caaatactga gacgcaagtc
    16321 acagggaaag atccagaagc ttatccgacg ttcaaagaat ttttagactt tttaggacaa
    16381 ctggaattac caggtgcttc ccaagagaaa aagaatcgaa aagaagaaat ggaatccatc
    16441 gtagaatcct tatgtgagac gtatggcatg atttttgatg gtcacagtac gattcgacac
    16501 cttgaccaac aacagttggt cttttttgac attgactcta ttacaggctt agatcgaggg
    16561 gtacaacaat gtcaaatgta catggctatg acgttgattt ggaatcaggc attgattcaa
    16621 ggaagaagac aacgacgttt acttaaagaa aaacaaatgg aagaacaaga cttcaaacgc
    16681 ttcttggcga tgattgatga gtgccataat ggtgcgacct gttctgcagt gaaaagctgt
    16741 gggttgcaag tagtctaaaa actattgtaa taactcttat aacgagaagt caaatgagtg
    16801 ggtaacgcca caaagggctt ccactgatac tccgacatgc aacaagtagt ttatgcagac
    16861 caacgttgtg tgaagctcgg tgaagtcggc tgaaggatac cgtaaggtaa aagaaatagt
    16921 gaataccacg aaagttcttt agaggtaaag ggtgtatccg ataagtcggg tgtccaaaaa
    16981 aacttaatat ggtgagtatg tacaaacgta ctgacgaact agcgaatgta ctgtccgaaa
    17041 ggcatagctc ctagaaatgg gagagacagt gagcaaaaaa tcactcattg tgctaacttg
    17101 tggttgagta aaagtcgttg ttatgaaaga ccatgctgcg ttacaggcgt aggagaagtt
    17161 agggggcaaa agctagcatc taagttaagg catgtggata gatataaggg aacaggggaa
    17221 gccatcaaca tggagagtgc atactcgatg aagtggtagt aagaaatatg tgaatgacaa
    17281 ataacttact ttagtgatgg tgaaagtaga gtcacgactg atgaaggttc tgtaatggaa
    17341 ctggaggaac aggctcgatt cgtctgtaaa agtcatgaaa cccgttcaac catgggattc
    17401 tcgtatgact aaaaaggtca cgagccaatt ggagcgtgat aaaccatgac aaaagaaaca
    17461 agtccaaaag ataggaagtt gcggcatgca gaatattatg gcatgacgga aatcttcgat
    17521 aagatatact ccaaaagttt gaagggagat aatttcaaga atctaatgca gattatcatt
    17581 tctgataaca acattgtcct agcatatcgt aatatcaaga gaaatcacgg tagtgtgaca
    17641 agaggagaag atggagtaac gattaaagat attgaaaatc tcaaccgtaa tgaattcgta
    17701 gaaattgtta ggaagcgatt cgcacattat aaccctcgga aagtaaggcg tgtagaaatt
    17761 cctaaatata atggtaaatt aagaccgttg ggtattccat caatatggga tagaatcgcg
    17821 cagcaatgca tcttacaagt attagaacca atatgtgaag caaaattcaa taatcacagc
    17881 tatggtttcc gcccaaatag atcagcagaa cacgcaatag cagattgtat ggcccgaatg
    17941 aatcaaagtc acatggaata tgtggtagat gtagatatca aaggtttctt cgacgaagtc
    18001 aatcatacta aactcatgag acaaatctgg tctatgggta tacgagacaa acagcttctc
    18061 ttaatcatta ggaaaatgtt aagagcaccc gtagtacttc caaatgggaa gttgatatac
    18121 ccaacaaaag gaacaccaca aggcggaata cttagcccat tacttgcgaa catcaatctc
    18181 aacgaatttg actggtggat agcaaatcag tgggaggaaa aagatgccaa agagctaaaa
    18241 catcatttta ggaaaaatgg agcttgggat aaaggtaata tctatcacaa gctcagaaag
    18301 agtacaactc taaaagaaat atacctagtg agatacgcag atgattttaa aattttctgt
    18361 agaaattata atgaagcgaa aaaagttttc tatgcaacta aaatgtggtt acaggagcgt
    18421 ctaaagttac caatatctga agaaaagtca aaaatcacaa atctttcaaa agaagaaagt
    18481 gaatttttag gctttacgat gaaattagtg accaaaggta acaaaaaggt tgttaatacg
    18541 catataggta caaaagcaca aagcatgatt aaagtgaaac taaaaaaaca aatcaaacga
    18601 attcaaaaaa gtcctaatag caaagatacc tttatctata ttggagtcta taacagtatg
    18661 gttattggga ttcataatta cttcaaaatt gcgactcatt gtagtgtagc aatgcgaaaa
    18721 attgcaagag aactcagacc aagttttgaa atacgactac gagaacaagg aatctcaaaa
    18781 gaaggacaat acggaaagta cgaaggatac aagccttacc tacggtcagg acaaatacga
    18841 tatataagag ggtttccaat attaccagta gggtatattc aacacagaca tccgatgtgt
    18901 aaaaaaagaa atattaataa atacaccgaa gaaggtaggg aagccattca taagaagcaa
    18961 caaaccgtag aagaatggaa gatatcgtgg ttgtgtagca atccagtgct tagtaagaga
    19021 gcaacgattg aatttgcgga caatcgaatt tcaaaattca tcgctcaaaa gggaagatgt
    19081 gcagtaacag gtgaggagct tatcttatct gaaatgcatt gtcatcatat tatcccttat
    19141 catgaaagca aaagtgatag ctatgaaaac ctagtgattg ttactgagga ggttcatcgg
    19201 gtaatacacg ctacacaaag tgaaacgatt gaggaacttc taaaatatct aaaattaaat
    19261 ccaaaacaaa aagaaaaact caatgagttg agattaaaag ttggtaacga agaaatatct
    19321 taataaatat ttgtaggacg ggaaaacaga atgtctttta tagacgaggg aacgccgtat
    19381 gcggtgaaag tcgcacgtac ggtgtggact gggggaaaag acggagataa tatcaaactc
    19441 ttacctatca gtatcgatta atccagattt tattgaaacg gtaaattatg tcacaaactt
    19501 tcaacgagaa atgcgtaaag tagaagcaat gactgtttta gctacacaat ccccacaaga
    19561 aatggtgcca gaaaatatgt cttctgataa cttctcgaaa ctgaagaaag tctttgaatt
    19621 ttcttctacg aagatcttta tgcgtatgga tgaatctatt ttacgtcata tagaaggatt
    19681 agttggtaaa tcaatgacga actcagaact agaatccatt cctcaacaaa atcaaggaga
    19741 cgctgtgatc aattttggct caaaagaaac cattcgtgtt cattt
//
LOCUS       DAFEOM010000046        19646 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.047, whole genome shotgun sequence.
ACCESSION   DAFEOM010000046
VERSION     DAFEOM010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.047"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(28..726)
                     /locus_tag="LP524_001844"
     CDS             complement(28..726)
                     /locus_tag="LP524_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HBL2234881.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(719..2071)
                     /locus_tag="LP524_001845"
     CDS             complement(719..2071)
                     /locus_tag="LP524_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HBL2234882.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2294..3694)
                     /locus_tag="LP524_001846"
     CDS             complement(2294..3694)
                     /locus_tag="LP524_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HBL2234883.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3855..4592
                     /locus_tag="LP524_001847"
     CDS             3855..4592
                     /locus_tag="LP524_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBL2234884.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4717..5196
                     /locus_tag="LP524_001848"
     CDS             4717..5196
                     /locus_tag="LP524_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HBL2234885.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5193..5960
                     /locus_tag="LP524_001849"
     CDS             5193..5960
                     /locus_tag="LP524_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234886.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6190..6759
                     /gene="lepB"
                     /locus_tag="LP524_001850"
     CDS             6190..6759
                     /gene="lepB"
                     /locus_tag="LP524_001850"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBL2234887.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6863..7465)
                     /locus_tag="LP524_001851"
     CDS             complement(6863..7465)
                     /locus_tag="LP524_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBL2234888.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7571..8040
                     /locus_tag="LP524_001852"
                     /pseudo
     CDS             7571..8040
                     /locus_tag="LP524_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8146..8730
                     /locus_tag="LP524_001853"
     CDS             8146..8730
                     /locus_tag="LP524_001853"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBL2234889.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8733..9806
                     /gene="prfA"
                     /locus_tag="LP524_001854"
     CDS             8733..9806
                     /gene="prfA"
                     /locus_tag="LP524_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBL2234890.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9799..10638
                     /gene="prmC"
                     /locus_tag="LP524_001855"
     CDS             9799..10638
                     /gene="prmC"
                     /locus_tag="LP524_001855"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBL2234891.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10631..10810
                     /locus_tag="LP524_001856"
     CDS             10631..10810
                     /locus_tag="LP524_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234892.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10832..11857
                     /locus_tag="LP524_001857"
     CDS             10832..11857
                     /locus_tag="LP524_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBL2234893.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12032..13276
                     /locus_tag="LP524_001858"
     CDS             12032..13276
                     /locus_tag="LP524_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBL2234894.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13429..14376
                     /locus_tag="LP524_001859"
     CDS             13429..14376
                     /locus_tag="LP524_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL2234895.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14510..15139
                     /gene="upp"
                     /locus_tag="LP524_001860"
     CDS             14510..15139
                     /gene="upp"
                     /locus_tag="LP524_001860"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBL2234896.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPGGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15278..15520)
                     /locus_tag="LP524_001861"
     CDS             complement(15278..15520)
                     /locus_tag="LP524_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234897.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15641..15713
                     /locus_tag="LP524_001862"
     tRNA            15641..15713
                     /locus_tag="LP524_001862"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15674..15676,aa:Thr,seq:cgt)
     gene            complement(15832..16470)
                     /locus_tag="LP524_001863"
     CDS             complement(15832..16470)
                     /locus_tag="LP524_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBL2234898.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16593..17225)
                     /locus_tag="LP524_001864"
     CDS             complement(16593..17225)
                     /locus_tag="LP524_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBL2234899.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17576..17875
                     /locus_tag="LP524_001865"
     CDS             17576..17875
                     /locus_tag="LP524_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBL2234900.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            18370..>19646
                     /gene="acm"
                     /locus_tag="LP524_001866"
     CDS             18370..>19646
                     /gene="acm"
                     /locus_tag="LP524_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HBL2234901.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLP"
BASE COUNT         6799 a         3369 c         3870 g         5608 t
ORIGIN      
        1 tttgtcacat tccccttttt cgaataacta ggctgtttgt ccttcaagat agggcagatc
       61 caattcttta ccgtaacgtt gttctaaagc taggcggatc aaacgagctg ccaggttctc
      121 atttcttgca aggataggtc catggaaata agaaccgaag acatttcggt aaatgacacc
      181 ttcagattgg tcttcaccat tatttccttg accttgtacc actttcccca aaggtcgttc
      241 accttcacct aagaaagtcc gaccattgtg gttttcaaat ccataataag tttcattgaa
      301 ttcttcgtta tggataacga tatcaccgat aaaccggtta ttttcctgac ttaacgtgta
      361 gtgatccagt gcactgattc cttggatctt ttctccatta gctcccatgt agtagtgacc
      421 tagcaattgg tagccaccac aaatggctaa catgacgcca tcattttcga tatagttgat
      481 caaagaatct ttctttttct ggatgtcttt tgaaatgatt aattgctcaa agtcttgacc
      541 accgccaaag aaaaccaagt catatttatc agcatcaaaa ggttcatgta tactaacgat
      601 ctccgtatgg caagaaaccc ccattttttc tgctatatat ttcaacatca aaaggtttcc
      661 gttatcacca tatgtgttca tcaaatttcc ataaaggtgg gcgatattga gttcataatt
      721 agccacggtt cattcctcct ttgataaatc cttgagcagc tagttcttta cgaagctgca
      781 atacagcagt gtaagtagct aaaatataga catgctcagt tggcaattct ttgatttttt
      841 cgattacttc tggtaattct tttgtttctg tcaatttatt ttctggaatc ccggcaactt
      901 ttagacgtaa agtcatatct gtatgacggt ctcctccaga aataacagct gggatatcca
      961 tatcggcaaa gttttcaaaa ttaccatccc aaatccagct gacatcgatt ccgtctgcgt
     1021 aattggcatt taataaagcc actaaagaaa atggataagg agctagacca atcatatcga
     1081 ttacttgatt gatacctaca ggatttttga caagtactaa tgtacatttt ttcccattga
     1141 tattgatgac ttcttgtcgg ccgaatactt tctcgtcata tcctaaacct tgtttgattt
     1201 tatcaggatc gacattgaag tactctgcca ctgcagtagc tgctaaagca ttgtagacat
     1261 tatacatacc gccaacttcg attccgtatt catgaccatc gatcacgaaa tctgctgaca
     1321 cattgtccat ccgtaccata tcagttaatc gataatccaa ttcaggacgt ttgaagtcac
     1381 aatttggaca gtaatatttc cctaagtttg cataagtgat cattttataa tgcaagatgt
     1441 gatgacaatt tggacataag acaccgtctg tattgtaatg agccatttgt tcacgatctt
     1501 cttcatggtc aaagccataa tattttctag gattgactgt ttcgatagaa ttgaagattg
     1561 gtgaatcacc gttactgata atcgttgcat ttggtgactt tgcggcaccg tctacaatca
     1621 ttttataggt cgtatagatt tctccatacc gatccatttg atcacggaaa atatttgtaa
     1681 ataagaacag ttctggtttc atatattctg tgactttgct caaacttgct tcatctattt
     1741 ctaggacagc aaactttttc tgtccttttt ttggttttgc ctgcaagaaa gtcgatacaa
     1801 ttccctgtac catattcgca ccagtagggt tggttagaac ttcatcgaat tgctggcgca
     1861 aaatattgac tgttaaagcg gtagtcaatg ttttaccgtt tgttccggtg acaacgacga
     1921 cttggtagtc ttttgccaaa ctatctagta tatgtggatc gactttcagc gctagtttac
     1981 ctggtaaact tgatccgcct ttaaaaaaag tttttaatgc ccattgcgac gtttttccaa
     2041 ctgcaatcgc aaaatgactg cgtattccca ttatgtaacc tccaaaaacg atttgctgac
     2101 gaataaattc aactaatcat accatatgtt gaaaaaaagg ttaaattgct tttcttattc
     2161 ttaagaaaag gttacggctg aaagtgcatg aaatcggaca aaatcgcatg atttaacaaa
     2221 aaaacagctt gaaacaaaat gattgtttca agctgctgtg attttattat tccctcttac
     2281 ctatattccc attttaaccg ataataattt tctcgcttgg atattcatag cctagatctc
     2341 gtgtttcttt tggcacgaat aacatcaatg tatatagcat tccgatccgc ccaataaaca
     2401 tcagtgtagc aatcgtgatt ttgcccacaa cagacaagtc tccggtaatc cctaaagata
     2461 aaccagttgt tccaaacgca gaagttactt cgatgatgat cgagatcagc gtttgttctt
     2521 ctgtagcaga taaaaaaacg atacaaaaga aacacatacc aagagaaagc ataaagacaa
     2581 cgactgattt tcgcacatca tcttgatcga ttctccgtcc aaaaatattg atgttatctt
     2641 cacttttgag gaaggaatat agatacaaac cgatgattgc tacagtagtt gttcgaatcc
     2701 cgccgcctac agaactagga ctgcatccga taaacatcag taaagaaaaa atgatcagtg
     2761 ttgtgatttg aaaatcaccc aaatcgtgaa tctgcagacc agcattacga gtcgtaatcg
     2821 aataaaacat cgaattgatc catttgacac tcatattgga atcttggaat aaatggtcct
     2881 tttccaatag ataaatcaag acagttcccg aaacaaacaa gataacaaaa gctaacacag
     2941 ctaatttggt aaacaaggaa aaacgaaacg gaagcttcgc attggaacgt ttgtataata
     3001 gccattcccg gcactccatc aatacaggaa aaccaattcc tccgacaaaa atcaaaaaca
     3061 tgatcaggat caagaaaaaa tagtcatgtg caaagggctt gatggaatcc ccagtgacgt
     3121 caaacccaga gttcgtgaca gccgatatcg cctgataaaa accgtaaaaa acggcatcac
     3181 gccaacgatc aaaatagcct ctgtaataaa aataaataga aaagaatgtc ccaaaaagta
     3241 attgaaacca gattaaaata gcaaaagtga tccgaatcag tcggacgata ccagacaaac
     3301 gcggttgatt catatccgtc atgatcagct gtcgctgttt cagtgtgatc cgtcgtttag
     3361 aaaaaatgac aaaagcagta gaaatcatca tgatccccaa accaccaact tgaaataata
     3421 tttccagcag tataataccg ttatcattga acacagaatt gatatcaaaa gtcgatagtc
     3481 cagtcacact cactgtactg attgccatga acagcatgtc cacaaaagaa acatggctat
     3541 ctggttcgcg aaaaatcggc atataaaata gaacgagtga taagaccgtc atcaatatat
     3601 aataaaaaac gatgatttgg atcgatgaga aatgattggc agcaaacttc tgtcctcgcc
     3661 gctgcagcct tcgaaagaat aaatcaacat gcacagtact atcctcctga caatagtcgt
     3721 ttctaccagt atacaagggg aattaggaat actcaaagaa attatcaaac agataacaat
     3781 acagctttcg attaaaatag aaatggctta taataaggaa aatcctagaa aaaagaagga
     3841 ggaggaataa caaattgatt aatgctcaac aaatcgtaga aaaaatgaaa aatcaaaaaa
     3901 taaattatga tacagttctg aaaaaaatga tcatgcaatg ggagaaagaa gaagtaaagc
     3961 ctaagatatt aatacataca tgctgtgctc cttgcagtac gcacacattg gaattcatgt
     4021 ctcaatatgc agagatcacg ttattttttt caaattcgaa tattcatcct aaaagtgagt
     4081 atttgcgaag attacatgaa caaaaacgat ttgtagacca atttaatgag aaaactgacc
     4141 atcatgttgc gctaatcgtt gatgaatacc gtccaaatga attcaccaaa atggttttaa
     4201 caaaccatct tgaaaacgaa aaagaaggtg gtgctcgctg cagtgcatgc ttcaatatgc
     4261 gattagatct tgttgcgcaa aaagcgcaag aactaggata cgattacttt ggaagtgccc
     4321 tgaccatctc gccaaagaaa aatgcagctt tgatcaatca gatcggaatg gatatccaaa
     4381 agatttacgg gacacaatac ttgccaagtg atttcaagaa aaacaaaggc tatgaacgct
     4441 ctttgcaaat gtgcaaagag tatgatattt atcgccaatg ttattgtggt tgcgtgttcg
     4501 ctgccaagca gcaaggatgc gatctgaaag aaatcaatcg ggaagcaaaa gcttacttaa
     4561 aaacatcaac cgtagttttt gaggaaaatt aaagaaatct ttcggaaaaa cgtaagaaaa
     4621 aaataaagaa tttatggtag aaataaggta gactgtataa tttgtccgta gtaaagtaag
     4681 gaaacaagaa taaatttcaa tgaaggaaga gataagatgc gaaaagtagc acctattttg
     4741 aagccaatcg ctgtattaac aatctcgacg attgtcgcga ctactacagg tattgtgaat
     4801 agttccagct accgtgcacc aagttcaagt tctagtactg tagaagaaac aacaccaact
     4861 acttcagtct cgacatctga accaacaaca acggaaagtt tgaatgaaac cactacgaca
     4921 agtacatggg acgatacaac aactagttcg tcgtatgatg agacatatac aactgacagc
     4981 tcaagcgaaa caagtaatac ggtaccagat ggcactccat cggattcaac caatccaagt
     5041 acaagtccgg atacaagtgc tagctccgaa acgataccaa gcagtacgac tgtagatgat
     5101 aacacgcaaa atgatgatgc agcacgtaca gcacagcaaa accaagattc aggaaatacc
     5161 caaacgtata accagcaagc aggtgattct caatgacatt tttactagac cttatcaacg
     5221 gggtgcataa attccttggc tatttagaca tcagtcctaa atatcttaac cgtggatata
     5281 cgattttaag tgtgattccc acgctttatt tattgagaat tgtttatgga ctttggcaaa
     5341 accaaaacta tcttcaattt ttcttatatg gacttgcatt tctagtgctt gtttatttta
     5401 ctgttttaaa tgtattttat tactttttag ataaaaacac gaaagccgat gtcactcagc
     5461 tttttgtgaa gtatttacct gatgaagcgt tcaacatcca gacagaagaa tcgatagata
     5521 atggaaattc gatcgataaa gtgaatacac aagaggtcat ggtttcttat gacgaagatt
     5581 atcaattgaa attagccgag aatatgcggt atttgattgg aaatggtgag atcaaaacaa
     5641 atgaactcgg tcgcatggat ggatttctgg tcgatagaaa tacattgtat ccttattact
     5701 atgtcaagaa aacaggagag aaagagtatg ttctaaatat tggacgaagc tactcggaca
     5761 tggaaaaagt agggaccatt cgacttgact caacagaaga cacattggaa cctgtcggat
     5821 tgttcatcgt cggcggtgat tttgtcaaag acgggttacg ctatcatgag ccttatcgtc
     5881 tgaaattgat tgcaaaaaag gcacagtcgc aggaagaacc agtggtttat agtcgtagcc
     5941 aaagaagaag acattcataa tgtgatgaaa cgccgtgtat cagtggttgc gataaaaacg
     6001 tttattcgga ataaggggga tgtgacacgt cttttgtcac gttccttttt tatataacga
     6061 gaaagataag acgaacacat cacttattct ttcctttcat ttataaacat tgtagaataa
     6121 agaaagtaca acgaagtatt gtgaaaaaac gtaataaata acgaggaaaa tacaagtagg
     6181 aggtaagctg tgaaagaatt tttgaaaaat tgggggattt tgatccttgt attagcggct
     6241 attttattag ctagagtgta cgtgtttaca ccagttactg taaacgggca ttcgatggac
     6301 cctacattaa gtgatggaca acgattgatt tcatctaaga tctcaaacta tgaacgaatg
     6361 gatattatta ctacaaagga accgggggat gaagaacgga tgattgttaa acggatcatc
     6421 ggtatgcctg gagatacagt caagatggaa aatgaccagt tgaccattaa tggcaaaaag
     6481 tatgatgaac cttatctgga tgagttcaaa aaagaatttt cagaagataa gttacaagga
     6541 gaatatgctt atagctcagg attccaagca caagcagaaa gttcatccac ctttacgagt
     6601 gactttgaat atactgtgcc aaaaggaaag tatcttgttc taggcgataa ccgcttgatt
     6661 tcaaaagaca gccgaatgtt tgggttagtc gacaaagata tgatccaagg aaaagttgtt
     6721 ttccgttatt ggcctctttc agaaattaaa atcatgtgat gaaaaaataa aaagaagctg
     6781 tgacatgttt tttgtcacag cttctttttc cgaaaattcc ttcttatttc ttggagttgc
     6841 tcactttttt tgcaatctca ttttaagctt tttggctgat ggataagaaa agcgaagcgt
     6901 ttttgttcat aaatagcttt tccccacgat ggaaggtatg actagtagca gagacaaagc
     6961 ccgttttttc caatcgatta ttcaattctt cttggctaaa gccattatgg actttcggat
     7021 gactaatttg ttcattcttg tcaaagtcta cgatgatcaa ttgaccgcca ggagctaaaa
     7081 tttcataaag ttttgtaaga atattctccg tatcaggaat gtgtaaaagt acaagtgata
     7141 gaaggatgag attagcttgg acagcaggaa attcgacaga agcatcagcg tggatcgttc
     7201 ggacattttt cagatctgct gcttggatct tttcttctgc cattttcagc atagtttctg
     7261 aagcatcggc aatgatcagt tctttgaatc tttccgcaag cggtaaacta acaagcccag
     7321 ttccgcctcc gtaatccagc aggacttttt ggtggctgtc gcgcggtaga taagtagtta
     7381 gttcttcgcg aataatcttg gctaatgctt gacggtcagg ggaatcataa tggttagcga
     7441 tggaatcaaa aatatttttt gtcatcaaat agctccttac gtaaaacttt gaaattttaa
     7501 cttattatag cacgtgaaaa gaaagaaagc atgctatact caattcaaaa agggagaatc
     7561 tagaaagaat atgccgatat cgtttgtaaa agatagagag gaaaaaggaa agtgtgtccg
     7621 agaaatatta ctagacttgc ctgagtggtt tgggcttcct gaatcaacgg aaaaatatat
     7681 cgaggaatca agtaaactac ctttatggtg tgaaaaaaga aaagaagaat atttaggctt
     7741 tatcactctt tcacagacaa gtgaagatac tgccgaaatc tattctattg tatgggaata
     7801 aaaaaagact atcatcacca aggattagga acaaaacttt ttaaagaagc agaaggttac
     7861 gcagcaaaac attacaaata tttgcaagtg aagactgtag acgaggggca ctactctata
     7921 tatgatcaaa ctatttgttt ctacgaatca ttaggatttt caaggttgga agtttttcca
     7981 aatttatggg atgaatggaa cccatgttta gttcttgtca aaaaattgga acaaaagtga
     8041 gcaatagatt taatactaat ctgtgcttga tgaaaattct cactgtaaaa aatgctgaaa
     8101 atcaagtata ataccatagg aacaaactgg aaggtgaaaa agagcatggc acaactattt
     8161 tttaagtatg gcgcaatgaa tagcggcaag acgatcgaga tcctaaaagt cgcacataat
     8221 tatgaagaac aaaataagcc agttgtctta atgacaagcg gtattgatac aagagatggt
     8281 ttaggcgttg tttcaagccg aatcggactg aaacgtaatg cgatgccgat atttgaagaa
     8341 acagacgtat ttgacgtaat tcagaaaatg gaaagcaaac cctattgtgt gctgatcgat
     8401 gaagcgcaat tcttaaaaaa gaaacatgtc attgctttta cgagaattgt ggacgaattg
     8461 gatattccgg ttatggcatt tggtttgaaa aatgattttc gaaacgagtt atttgaagga
     8521 tcaaaatatt tattgcttta tgcagacaaa atcgaagaaa tgaaaacaat ctgttggttc
     8581 tgccacaaaa aagcaatcat gaatttacat tatattgatg gaaaccctgt ttatgaaggc
     8641 gatcaagtgc aaattggcgg aaatgaagct tattaccccg tttgtcgaaa acattacttt
     8701 cacccatcaa tgattacaga aggagaataa gcatgtacga tcaattacaa gcaattgaag
     8761 accgttatga agagcttgga gaacttttaa gtgacccaga agtgatttct gatacaaagc
     8821 gttttatgca gctttcaaaa gaagaagcaa acacgcgcga aacagtcgaa gtttatcgtc
     8881 gttataaaga agtagtacaa ggaatcaccg atacagaaga actgcttggt gaaaaactgg
     8941 atgatgaaat ggctgaacta gcaaaagaag aattatctga attaaaaaaa gaaaaagaag
     9001 tcttagaaga aaaaatcaag atcttgcttt taccaaaaga tccaaatgac gataaaaata
     9061 tcatcatgga aatccgtgga gcagcaggcg gagacgaagc agcattattc gctggcgatc
     9121 ttttcaatat gtaccaaaag tatgctgaag cacaaggctg gaaaacagaa gtgatggaag
     9181 ctaacgttac cggaatcggt ggatataaag aagtgatcat gatgatcacc ggagacaacg
     9241 tatattcgaa attaaaatat gaaagtggtg cgcaccgcgt ccagcgtgtt ccttcaactg
     9301 aatcgcaagg gcgtatccat acttccaccg caacagttgt cgtgatgcca gaagctgaag
     9361 aagtagagat cgatattgaa gacaaagata tccgtacaga tatctatcat gcttcaggtg
     9421 ctggcggaca gcacgtcaat aaaacagcat ctgctgtacg tttgactcac ttaccaacag
     9481 ggatcgttgt tgctatgcaa gatgaacgtt ctcaaatcaa gaaccgtgaa aaagcaatga
     9541 aaatcttgcg tgctcgtgtg tacgataaga tccagcaaga agcacaaagc gagtatgatg
     9601 ccaatcgaaa atcagcagtc ggaactgggg atcgttcaga acggatccgg acgtataatt
     9661 tcccgcaaaa tcgtgtgact gatcatcgta tcggattaac tatccaaaaa ttagatcaga
     9721 ttttagcagg gaaattagat gagatcgttg atgcattgat catgtatgat caaacatcaa
     9781 aactggaagc tatgcaaaat gggtaaaatg acctatcgag aagtcctgtc acgggcttct
     9841 tcttttttag aggatcatgg gaaagaagca tatagtatcc aatttttatt tttggaacga
     9901 aaacaatgga aaaaacttga ttggctcctt catatgaacg aagagatttc agaagaagaa
     9961 cagcggttga tcgaaaccga tctgcgactt ctgcttgcag atcatccacc tcagtatttg
    10021 ctaggatatg cagattttta tgatcatcgg ttaaaggtaa ctgaagcaac actgatccct
    10081 cgaccagaaa cagaggagtt agtcgaatgg tgtttagacg agacgcctgg tgtaccgtta
    10141 gaagtgatag atattgggac agggactgga gcaattgcta ttagtttgaa agcagcaaga
    10201 aaaaattggc atatatctgc tgtggatctt tcagaagaag cattagaagt agcgaaagaa
    10261 aatgctcaga aagaaggaac gaaaatcagt ttttatcatg gagatacatt aggaccagta
    10321 atggatcagc aatttgatgt gattatttct aatccgccgt acatcagtcg gaatgaatgg
    10381 gaactgatgg atgaaagtgt ccggtcattc gaacctaaaa tggccctttt tgctgagaac
    10441 gatggattag ctatttatga aaaaatagca gttgaagcac caagcgtact aaaatcaaat
    10501 ggaaagattt ttttagaaat cggatttcgc caaggtgaag cagtaaaaaa aatatttcag
    10561 caagcatttc cagataaaaa agtggcgata aaaaaggatc tttttggcaa tgaacgaatg
    10621 atccaagttt atggttgaag ttgtggatga aaaatttttt atttgttttt ttattataaa
    10681 aaaacactta tttgacaagg atgtaaacga ctttttggtt tatcaacaag tttatccact
    10741 attgacaaat agattatcca caaacagtgg aaaacttgag gataaactca gacaaaataa
    10801 gaatgtatga aaaaggggtt gtggataaac tgtggaaacg aaacgatatg atgaaacaga
    10861 attgcaagag gcagccgaag cactgaaaga aggagaactt gttgcttttc ctaccgaaac
    10921 agtatacgga ttgggcgcca acgctctttt accaaacgct gtcaagaaag tgttttctgt
    10981 aaaagggaga ccgcaagata atccattaat cgtccatgtc gcctcttttg aacaagtaaa
    11041 agagtatgtg gacaactttc atccggcagc agagaagatt gtgaagaact tctggcctgg
    11101 accattaact ttgatattta agatcaaaaa agacactttg ccctcagttg tgacgggagg
    11161 attatccaca gctgcatttc gtataccgga taacaaaaaa acactagaag taatcgaact
    11221 ttccggtgtt ccgcttgtcg gtccaagtgc aaatacgtcg ggaaaaccaa gtccaacgac
    11281 tgcagatcat gtatatcatg atctacaagg aaaaattacc gggattattg atgatggagc
    11341 tacgcgaatt ggtgtagaat ccactgttct tgatttaagt gatccaacag ccatgcctat
    11401 gattttacga ccaggtgcag taacgaaaga acaaatcgaa gcagtcatcg aatctcctgt
    11461 tgctattgat cagcatcttg taaaagaaaa tgaaacacca aaagcccctg ggatgaaata
    11521 caaacactac tcacccgata cacgtgttct gatggtcaga gatggcgatt ggtcaactgc
    11581 agttcaatgg gcaaaaaata agaagatccg tgcaggtgtc atagcaagcc ccgaaattgc
    11641 ggatcaagtg cggacagata ctgcagctgt ttatatgtat aacgacaact cagtagaagc
    11701 ggcggctaaa ggattgtttg caggtctaag aggattagat gaaccaacac ttggattaga
    11761 tttgattttt gtacaagtgt atccagaaac tggattgggg aatgcttaca tgaatcgctt
    11821 gaaaaaagca gcaggacaaa attattttga aaaatgatca attggttatt gtcgcattct
    11881 tttagtatga tacaatgcta tcaagtaaat cttctatcag tgaacgaaaa actaagcgta
    11941 actaatcgta atagaagaga tacgaatatt tggcaatttg tttttgatgg gaaaaataaa
    12001 attgtaggaa aataaaaagg agctgttgtt tgtggtagat tacaaaacgt ttgaccctga
    12061 tctttgggct gcgatcgcca aagaagaaga aagacaagaa cataatttgg agctgattgc
    12121 ctctgaaaac tttgtctccg aagcagtgat ggctgcacaa ggaagtatct tgacaaataa
    12181 atacgcagaa ggttatccag ggcatcgcta ttatggtggg tgtgaatttg tcgatatcgt
    12241 tgaaaattta gcgattgacc gtgcaaaaga attattcgga gcgaaatttg cgaacgtcca
    12301 gcctcactcc ggctctcaag ccaacacggc tgcttatctc gctttggtag aaccagggga
    12361 cacgatctta ggaatggatc tttccgcagg cggacatcta acacatggat caccagtcaa
    12421 cttcagtggc aaaacctatc actttgttgc ttacggtgtt gatccgacaa cagaagttat
    12481 tgattataat gtggtacgta ttttagctag aaaacatcaa ccaaaattga tcgtagcagg
    12541 tgccagtgct tacggacgaa cgatcgactt tgcgaaattc agagaaatcg cagatgaagt
    12601 aggcgcaaaa ctgatggtag atatggcaca tatcgcagga ttagtagcag caggacttca
    12661 tcctaatcca gttccttatg cagatatcac aacgactacc actcataaaa cattgcgtgg
    12721 ccctcgaggc ggcatgattt tgacaaacga tgaagcatta gcaaaaaaaa taaatagtgc
    12781 ggtatttcct ggtattcaag gcggccctct agagcatgtg attgcgggta aagcagtagc
    12841 attcaaagaa gcattagatc ctgcatttaa agaatatagt gaacaaatca tcgccaatgc
    12901 aaaagcaatg gtcaaagttt tcaatcaagc aattggaacc agagtcattt caggggcgac
    12961 agataatcat ctgatgttga tcgatgttcg cgagttaggt ataaatggaa aagaagcaga
    13021 gagcattttg gacagtgtga atatcactgt aaataaaaac tcgattcctt ttgaaacact
    13081 aagtccattc aaaacaagcg gtattcgaat cggcacgcca gcaatcacta ctcgcgggtt
    13141 caaagaagaa gatgctgtaa aagtggctga actagttgtc aaagcattac aagcaaaaga
    13201 tgataacgct caactagacg aagtcaaaac aggtgtacgc gaattaactg aaaaatttcc
    13261 gttgcataaa aaataaaaat ttaaattcca ttagtaaaca gaaaagttca atgttgggct
    13321 tttctgttta ttttttggga aaacggattc tttgacaata tcggtgatgc tctctagtat
    13381 atgaggtgta attttagtct tcacactaaa tctcagggag gaaaagtcat gactgatcgt
    13441 atctcgttag aaaaggaagc agtagcattc agtgatgcaa atgcgcctca tccgcgtatc
    13501 tatgaactag caccggaaga aggaagagtt ttactagaag aagtacaaga atcacctgtg
    13561 aacaaatatt ccgtcgatat tgaagacttg atggtggata ctggcgaatg gggaaaactc
    13621 aatattcgtc tcgtacgacc agaagggaat atggataaat taccagttat tttttatatt
    13681 catggagcag gctgggtttt tggcagtgct caaacccatg ataaattgat cagagaactt
    13741 gcggttcgta cgaattccgt cgttgtcttt ccagaatata gtcgatcacc agaagcaaaa
    13801 tatccaacag caattgaaca aaattatgct gtgttgcaac agctgaataa attagccaag
    13861 tcaaaaaatc tggatctcga gaatctaatc gttgcaggag attcagtagg tggtaacatg
    13921 gctacagtca tgacgatctt aacaaaagaa cgaaagggcc tcccaatatc caagcaattg
    13981 ctgtattatc cagtcacaaa cgctgatttt gatacagatt cctatcatga attcggagaa
    14041 aactattttt tgacaagaga aggaatgatg tggttttggg accaatatac gacgaatgaa
    14101 gaagagcgtg ctgaaatcac agcttcgcct ttgagagcaa atataaatga actaaatgat
    14161 cttccttcag caatgatttt aacgggagaa gcggatgtat taagagatga aggtgaggct
    14221 tatgcgcgca aacttcgtga tgcaggggtt cctgttacac aagtacgctt ccaaggaatc
    14281 atccatgatt ttgtcatggt aaatgctatg gatcagacaa aggcaacgcg tgcagcaatg
    14341 gcattatcta cttcttggat caagaaaaaa gaataagctt tgcttgtccg caggaaaact
    14401 tcttttcaaa aacggatttt cttaggaaaa atggcgagtt tgactagtca atcacttgat
    14461 tttgttttac aattaaaaag aaacgacaga acaaaggaga gcttaaaaaa tgggcaaatt
    14521 tcaagtgatc gatcatccat tgattcaaca caaactaact attattcggg acaaaaactg
    14581 cggaacaaag gtttttcgtg aagtagtcga tgaaatcgct atgttgatgg catatgaagt
    14641 atcacgggat atgccattgg aagacatcgt gatcgaaaca ccgatcactg aaacaacaca
    14701 aaaaacgttg tctggtaaaa aagtagcgat tattccaatc ttgcgcgcag gtattggaat
    14761 ggttgacggt atcttggaat tgattccagc tgctaaagtg ggacatatcg gtctttatcg
    14821 agatgaagaa acattggaac cacatgaata tttcgtcaaa cttccagaag acattgatgc
    14881 acgtcagttg tttgtagtag atccaatgtt ggcaacaggc ggatcagcaa tcatggcaat
    14941 cgatgcattg aaagaacgtg gcgcgtcgaa cattaaattt gtttgtttgg tagcagcacc
    15001 tgggggagta aaagctttgc aagaagcgca tcctgatatt gacatctata cagcttctct
    15061 agatgaaaaa ttgaatgaac atggttatat cgttcctgga ctaggggatg cgggtgaccg
    15121 tttgtttggt acaaaataag taaatacgaa aaagggtgtg acacaacttt tgtcacatcc
    15181 ttttttggtc atttttaacg tttgatgcag aacagctttt tcacagcttt cgtttgcata
    15241 taaaaaaacc atcccaaaaa tgcagagatg gtcaaattca aatctttttt agttctttgt
    15301 agttaacgat tactacaccc ttactttgcg tcatcaagtt cgcattttct gcagtttcat
    15361 caatctcaac aacagcggaa tttttcaatt gctttgaaat cgttccagtt tgcgacttgc
    15421 cgtgaattgt gaacattacg tgagtattga tcttgaattt ttcaccaatt tccgatggta
    15481 cgatagttgt atcaaatttt ccaaaagttg ccattttcat aaaacctgcc tttcacatca
    15541 attataccac gttttatgaa aaatatcatg aaattaaact aattttccat cggactcttt
    15601 acaatcagcc aaatagagta tataatttct tttggataag gccagcttag ctcagttggt
    15661 agagcaacgc actcgtaacg cgtaggtcgt aggttcgatc cctatagctg gcattaaaag
    15721 agaagacaga tatatcaagg atttttaatc cattgataca tctgtctttt tgtttaaagg
    15781 aaataaagta tgctttctta gcaatagagc atgtattatt tctataaaaa tttaggagaa
    15841 taaaacgact agcaatatag ctgtgaaatc aaatatcaca tgaactgccc aacttgtaat
    15901 gatcgagttg cttttaatgg cagcttggtt gaataacaat cttgccaccc cttgtatcaa
    15961 aaggatgtgt gctacagtgt gtaaaatatt tccattatca taagtactgt aatggataaa
    16021 tccaaagatg attgctgaca agaaactagc gacaggaatg ctgaacttac tagaaaaaat
    16081 agcgagaaaa tacactgaga acaattcttc tccaagtatc atgataggca aaataataaa
    16141 taataacggg ctgaaagcct catttattgg attgcctgtt aaaccccatt tcaaaatttg
    16201 ttgcaaaaat accgagactg cggcactgat tactacattg ataaaaaaat atttggctat
    16261 atttttgaca ggagcgacag gtttcgagaa catttttttc gtatcttttt taccaaaaac
    16321 aagtgtaaat gctataaatc cagtgatagt taaagaaaaa atgctaataa tggatggcgc
    16381 aaatagcaac cctaacaaac cgagtacaac gacaatagtg cccaatatcc cttttttaag
    16441 ttctattttt tttcctaaaa tgttattcat gataataact cctactattc agtttttgaa
    16501 gatagagtca gtatacatga agagttaaaa taggcaatgt ttttttatgg atatttgtat
    16561 tgctaaaaat ttatcttata tattcatttt gattatacta cttttatttg taaaaatttt
    16621 ctagcaaaac caagtgcttg caactgatta agcgtacaat tttttagatg ttccgagcgt
    16681 acttcaatca tttcaaagga gttgctactt aaatcgatat tttttaaatc agtatcaaaa
    16741 aagctagcat tttctaaatc attagtagaa aacattattt tagtgaggga acagtctaca
    16801 aaatctgcgc ttgataagac agagtgctca aaggtacttt cttttatgtt tgaaccagat
    16861 aaatttacat agtccatttt acaattctcg aataacgtgt tcccaatata actttccatc
    16921 aatttagtac ctgttaattt acaattgaca aatttcacgc gatgcaacat gctttttgtc
    16981 aatatacagc ctgaaaaatc acagttttcg aatatacagt cggtaatctc cattctttga
    17041 aaatcattgg aagcaaaaaa tgcatacttg aaaatagatt tgtaaataat tggatggcag
    17101 attatttggt tttcgatgac ctctttttgt gaaaggatat tatacagttc aaaatcttcg
    17161 ttttcataga tatgaaaaaa ggatccttta ttcaagcttg cttcattaac tttaggaaaa
    17221 atcatattta attacctcca ttttcataaa gtcatattaa caaaagctaa accaacttac
    17281 attagcagaa aatgagatag gttttcgcct ttttcgataa cgatagtgtc gctatctctt
    17341 gaaacgttct tagaattgcc ctgagagaca caaattaggc gtaattctaa taaaaatctt
    17401 agaataaaaa tttttactga tttaatttag aatgttttta aaataaaaat cgttgccata
    17461 acagtgtatt gaaaggtttt aacaatccag taatgattta aaaaaatcta gatgtagcgt
    17521 atactttgtt tatagaatgt agtgggaaga aatatatttt tgggaggaaa tcgtgatgtc
    17581 taaaagtgaa gaaatagaaa acatggtact aaaattatat gatctcgttt taaatccaga
    17641 gatcaaagaa aaggaacgta tacttttgat agacgcaaag acaggcttag aaaaaggtca
    17701 gtattatcct aaagtcataa ataatttgga acgtagttta cgtccgttag caatcagggg
    17761 agaattgtct aaaccagtat caccgtttta tatggagata tcaacaatag gaaaatttga
    17821 aaaagaatta ggtcgaggaa tggcttcggc accgattact tttgggcatt tataaaaacg
    17881 agttattcat ctcaatgaat taaaaagtat agtcaatctc ataatgcagg ctaggttcat
    17941 ttatttgaac agagtctgta tttttttatt ctaaataaag aaacagatgt ttctttatat
    18001 tgtattttat cctttaatta acacaaaaac agctagtata aactaataat gttaattaaa
    18061 agtgtatttt atgaaactat cgttatttat taatgttgca tttttgtaag ttaaaaattg
    18121 gaaatataga ttgaaaaaat gaaagcaata agtaaaataa aatgaatata tgcgaaaaaa
    18181 taatttacat taggaaaaac cttgtaaaaa cagttttttg gcatatttat tttttataaa
    18241 tatttgtaaa ataactatca attttttagt taaaagattg gaaatgagtg ggggaggaat
    18301 tgaagaaagg atttttgaga gatgatatag taggttttat ttttagttaa aataggggag
    18361 atagaaaaaa tgaaaaagtg tagaaatatt attttatcaa tgttatttat tattactaat
    18421 ataacatcac tgattcctgt tcatgtctat gcggatgcag gcagagatat cagcagtaat
    18481 gtcacttcgt tgaccgtgga cccaacgaat attacagatg gaggaaacat aaaagtaaaa
    18541 ttctcgtttg acgaaaaaaa acaaaatatc caaccaggtg attatctttg gatcaattgg
    18601 ccaagtgaag gaaatattcg cggtgaagga ttccaaaaag aaatcccatt aatgattgaa
    18661 aataaaaatg ttggaacttt aacggtcaga aaagattctg cgcaggttgt gtttaatgaa
    18721 aatattaaaa acctagactc agtagaaggt tgggggcaat ttgagataca ggccagaaac
    18781 gtaaccgata ccggtgaaga aaatacggga ccattcaccg tgacgagcgg tgataaaaca
    18841 gctactgtaa atgtgacaaa gccagcttct ggttcttctt ctagcgtttt ttattataaa
    18901 acaggagaca tgctacctga agatactaag cacattcgat ggtttttgaa tattaacaat
    18961 aacggcactt atgttgaaca accagttaaa atcagtgatg aaatccaaag tggacaaaga
    19021 ttagatccaa gtacgtttga aattaatcaa atacacttag gtgaacagaa ggtttataga
    19081 ggagaagagg gaatccaaca atttttacaa gatttcccca gcgctacatt taatttttca
    19141 gtaactgata attacattga aataactata ccaaaaaact ttgtcaatct taggaaaata
    19201 atggtgagtt ataagacgat catagaaaac ccagaacaaa taaattttga gaatcattcg
    19261 gaagcttggt ttaaagaatt taataaacct gctgttgatg gagaaagttt caatcataca
    19321 gttaaaaata tttcagcttc tggtggagtc aacggtacag ttagaggcga attgaagatt
    19381 ttcaaatata tcaatgacac agagattggt atcccaaatg tgactttcga attaagacga
    19441 gcagatgaac agcctattca aggacagtct agtatcctgt tgacttcgaa tgaacaggga
    19501 gaagctagta tcaaaggcct gcaagtagga gactatgttg taaaagaaaa agaagcaccc
    19561 aattggattg attttgatcc actaagttca aatgaactca aattctccat aaacgaaaat
    19621 gatacagagg gagtcagttt acctat
//
LOCUS       DAFEOM010000047        19383 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.048, whole genome shotgun sequence.
ACCESSION   DAFEOM010000047
VERSION     DAFEOM010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.048"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            68..994
                     /locus_tag="LP524_001867"
     CDS             68..994
                     /locus_tag="LP524_001867"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HBL2234902.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            1176..2069
                     /locus_tag="LP524_001868"
     CDS             1176..2069
                     /locus_tag="LP524_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HBL2234903.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            2203..2328
                     /locus_tag="LP524_001869"
     CDS             2203..2328
                     /locus_tag="LP524_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2234904.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(2462..3094)
                     /locus_tag="LP524_001870"
     CDS             complement(2462..3094)
                     /locus_tag="LP524_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234905.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(3242..3784)
                     /locus_tag="LP524_001871"
     CDS             complement(3242..3784)
                     /locus_tag="LP524_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HBL2234906.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            3919..4308
                     /locus_tag="LP524_001872"
     CDS             3919..4308
                     /locus_tag="LP524_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234907.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            4312..5076
                     /locus_tag="LP524_001873"
     CDS             4312..5076
                     /locus_tag="LP524_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBL2234908.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPNIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            5205..6152
                     /locus_tag="LP524_001874"
     CDS             5205..6152
                     /locus_tag="LP524_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL2234909.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            6282..7022
                     /locus_tag="LP524_001875"
     CDS             6282..7022
                     /locus_tag="LP524_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234910.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(7313..7981)
                     /locus_tag="LP524_001876"
     CDS             complement(7313..7981)
                     /locus_tag="LP524_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL2234911.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            8115..8402
                     /locus_tag="LP524_001877"
     CDS             8115..8402
                     /locus_tag="LP524_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234912.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            8818..10356
                     /locus_tag="LP524_001878"
     CDS             8818..10356
                     /locus_tag="LP524_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HBL2234913.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            10353..11318
                     /locus_tag="LP524_001879"
     CDS             10353..11318
                     /locus_tag="LP524_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2234914.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            11305..12627
                     /locus_tag="LP524_001880"
     CDS             11305..12627
                     /locus_tag="LP524_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL2234915.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      12690..12794
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            12868..13611
                     /locus_tag="LP524_001881"
     CDS             12868..13611
                     /locus_tag="LP524_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2234916.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            13626..14618
                     /locus_tag="LP524_001882"
     CDS             13626..14618
                     /locus_tag="LP524_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL2234917.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            14630..15367
                     /locus_tag="LP524_001883"
     CDS             14630..15367
                     /locus_tag="LP524_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2234918.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            15357..16079
                     /locus_tag="LP524_001884"
     CDS             15357..16079
                     /locus_tag="LP524_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBL2234919.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(16163..16786)
                     /locus_tag="LP524_001885"
     CDS             complement(16163..16786)
                     /locus_tag="LP524_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL2234920.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQP
                     VVQETVQTEKQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYI
                     GRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            17181..17872
                     /locus_tag="LP524_001886"
                     /pseudo
     CDS             17181..17872
                     /locus_tag="LP524_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
     gene            18080..18370
                     /locus_tag="LP524_001887"
     CDS             18080..18370
                     /locus_tag="LP524_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234921.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT         6671 a         3191 c         3903 g         5618 t
ORIGIN      
        1 ataagtattg cttctttccg tttttttgct attattaaat aggagaaatc acaaaggagc
       61 gaatgacatg tcaaaaattt tagtttttgg tcaccaaaat ccagatacag atgcaatcgg
      121 ggcagccatt gcgttttcac acttgcaaaa tgaattaggt aaagaaacag aagcagttgc
      181 tctaggtaca cctaatgaag aaacacaata cgcattagac cactttcagc tatctgcacc
      241 acgagtagta gagtcagcta aagcagaaaa tgcagatgtc atgcttgtgg atcataatga
      301 attccaacaa agtatttcag atatcagcga agtaaatatt ttagctgtag tagaccacca
      361 tcgtattgca aactttgaaa cagctaatcc attgtactat cgtgcagaac cagttggatg
      421 tacaagtacg attattttaa aactatataa agaaaaccaa gtaacaattc ctaaagaaat
      481 cgcaggaatc atgctctcag ctatcatttc agatacattg ttgttcaaat caccaacatg
      541 tacgcctgaa gatgaagcag ctgcaaaaga acttgcagca atcgctgaag tcgatctaaa
      601 cacttatgga cttgaaatgt tgaaagcagg aacaaatcta agtgataaat cagctgcggt
      661 tcttcttgat ttggatgcaa aaagtttccc aatgggcagt tccaacgttc gtatcgctca
      721 agtcaataca gtggacctta atgaagtgat ggatcgacaa gcagagctag aagcagcaat
      781 ggaagatgaa atgaaagcaa atggctacga tctgttcgta ttagttgtca caaacatctt
      841 agacagtgat tcagaattac ttgttgctgg taaaggaaaa gaaaaagtag aagaagcttt
      901 ccaaacaact ttgaccaaca accgtgcttt gcttaaaggt gtcgtttcac gtaaaaaaca
      961 agtcgtacct caattgacgg ctgtatttag ctaatcaaat ggtagtatga agcattcaaa
     1021 atgaatggat aagcgattga tataaaaaga aacatagaac atactaaagg ccgaagaaat
     1081 ggaggagaat aacagtcaat tatgactctt gttcttcctc ctttttacgt aaaagccttt
     1141 taagtaaaat aaaacggata gaaaagagaa catatatgaa ctattattca aaaaataagg
     1201 aaaactttta tcaaaaactg accgatgatc cgcttttttc ttcattgacc gattatctat
     1261 atgaacatcg agaacaagaa acgattctaa gagaattgaa aaaagaattt tcgcaaaata
     1321 aattttccca ttttcttgat ttattgatcg atgcaggatt aatcaagagg gaagaacgaa
     1381 gatatcattt gaactttcca atatttgatc ctaaagatta cttgcaacaa gccacttcgg
     1441 ctgctgaaac catagctgaa caattgaaga gactctctgt tgatgaacaa aaattagcga
     1501 tgggagaagt tatttgggcg tattgtttcg aagatgaacg gaaagaagcg tatttttatg
     1561 gagtaagaaa cagccgagag acagaattgc tgaggacaac tgcaggaaat gagaagtacc
     1621 gttttatcac actttcctct atagaacact tcccgcttac tttggctaat tacttttttg
     1681 tacagaagaa tcagcttcct gtaacgaagg cattcaaaga attagccgaa cttattggag
     1741 acgtaaatga atcgtacttt tttgatcaaa tagaagttat cgttgacagg atacgtaaga
     1801 ataaatataa aaatcgccgg cctagtattt tccatcagtc tctacttgtg acgaatacta
     1861 taaaggaaga agaatcattc acattagaat tacctattgt cgaaaaaaac aattttgaga
     1921 ttgaattacc aacattggat ccttcattga caatggagga aatggccttt cttaaacgac
     1981 aaatcttttc tgagctgagc aagaaattta taccgcatgc attttcttat atcaaagaat
     2041 atggaacagt tttagtgagt aaaacctaag agagcagtaa gcaaaaatta tactgactct
     2101 tgcaggagga gcctttctct tcctttatgg ttaaaagaat tatttgagta ttttgcctat
     2161 ctaccaccta ttagattcac tacatggtct ggaaacaaag gaatgcaaaa aaatggtgag
     2221 aaatgtggaa tgacaaagga agtagtgata agaaaagtta gatttttaaa taatcaatat
     2281 tatgattcag tcaagtatgg cattttatgg gaagaactag ctgattagat taggaagtcc
     2341 atttgataat ccagaatcac taagaaacga atggcgagtc attaataatt tatccaatct
     2401 tgggttattc ttttagattg aagtgtttaa aactaaaaaa gcatttatga agtcgtaata
     2461 gtcaggcttc aaaatgcttt ttattttgtt tgttttttct ttgccacata aatacaccta
     2521 gactgacgat gaagagcata tccaacagat ttcctgcgac ttggatcgtc gaagggttgg
     2581 tcagtggcat taaagcaagg acaaaaaagg aaaacaagct gtaaataaaa caggtgacag
     2641 gaactaatcg aaagaagagc ggacatttca attggctaaa aatgagtgaa aaaataaaat
     2701 aagtaaaaat ggataaaatc acgactaaac cattcatgat aaagaagaaa agcgtattgt
     2761 gattaaacga ataaaaaaga attgctaaaa aagaaaacgt tttaaatgca atcaatacgc
     2821 ttgaagcttc gtttaacgta ccaggatgtc gatgaaaaaa ataggcaccg aaataaacag
     2881 aaaacagtaa gccgcaaaga gaagacaaaa tcaaaaaaaa cggattttct tcggcttctg
     2941 tgatatcaga atgatccaaa agcaaccagc ctaatgcact agaaggcgca tgtgaggtct
     3001 taaggtatga ataacataga aaaaataaga tcgtcaaagt tgcaaaaaaa gagcctaaat
     3061 gtaaaaaatt agtggttcta cgttgaattt ccataatatc tccaaattaa attgataagt
     3121 atataaagta taataacaaa ttgatgagta tatgtcgatg ttttttaatc aaaataataa
     3181 ataaaaactg tctaacattt aaaatttaat ttagatatta gacagttttt aagtttgtga
     3241 gttattttcc ttgtcgtttt ctcacttttt tagtccagaa accacctgaa atatattttg
     3301 gctcataaga aatgataaaa gcacgctctt cgatttcttt gatcaggcga tacagttcac
     3361 gttcgctttt tctaggagaa agtatttcga gaactagccg ctcaccttcc agccccattg
     3421 cagaagtctg tgtcacacca taaccgtgtt ctctcagtat acccggcaaa cttttatctt
     3481 ctgcgctatt tgctggcaaa atcgttgtga ccatgatgta tcctaatgcc aatttgtctt
     3541 caatcaaaat tccgacactg atacctactg cgtaacctaa cgcatagaca aataaattca
     3601 gtggattatc taaacgatct aaaacaaggg ataagccgac gatataaatc gtgatctcga
     3661 ccatactgac aagtggagca atcagtcgat agcctttcat tgtcaacatg aaacgtaacg
     3721 tgttcagagt gatataagca aaattaatga ggaagatcat ccccaacatt tttggatcaa
     3781 tcatttgtaa aactcctttc atttatataa caacaatatc aaaaaaaatc gtaagtgaag
     3841 aaaaattgaa actttataca tacaaaaaaa tgataaaatg attataatag aattataaag
     3901 ataggttagg tgattaaaat gaaaacggtc aatcgtgtgt tttgtgccag ccacgtgcta
     3961 agtctccttc tcagtatata cttaggtgtc tattcgattc aacagccaat tgaggtagag
     4021 aacattgttt ttcagatgtc gctgacagat ggtttaattt taagcgtttt tttctttttg
     4081 atactgttta ttggtaatat tttcggtagc gttgccagtt ttttgaattt ttctatttat
     4141 ccactccttt cgctagcatt aggagtggtg ggactttgtt ctctgtgctt atttcccgag
     4201 ccattcagtc ctgcatttat attatttggt acactgaacc tgttccagac aactgttgga
     4261 gcatggttgc tatggcgatc aggaaattta atgaaaatag gtgagtaaca attgaatcca
     4321 attatattga aacgttctgt ccatcatatt ccagtgttag aagttgttct gaaagaatta
     4381 agagaggaac gtattcccgt tgtcatctat tatcacggat ggcagacaag taaggagtta
     4441 gttttgacgc aaggaagaaa aattgcgcag cagggcattc gtgtgatcct gccagatgcg
     4501 atgaatcatg gggagcgcaa acagccggtt tcttctattc catcctatac attttgggat
     4561 agtatctacg gaaatctgtt tgaatttgac acacttatca agcatttaga aaaaagaggt
     4621 cttctacatg atcagcttgc agttggcggg gtttcgatgg ggggaatgac aacatgcgcc
     4681 ttgttggcta aacatcctaa tatagtagcg ggcgtgtgtt tgatgggttc ccctgcacct
     4741 ttggaatatg gaaaagaaat cgtaaggcgc gcatcggaat ataattatgt cgtaccaaaa
     4801 gactattttg atttgatttc atgggttaag agctatgatt tgtcattgca gccggaaaag
     4861 ttagctggac gtcctctgtt cttttggcat ggacaagctg acgaaaaaat cccttatata
     4921 caaacggaac gatttgtaga aaaaaatcgg cataagtctt acggaaaaga aattgttttt
     4981 cgttcctcaa aaacacagcg acatatggta caaataccac taatggaaga agcttccact
     5041 tttttagcaa aaaatttgat aaaaaagtat aaataattga aacggaaaat cactcatgac
     5101 gatagaggat tttccgtttt ttattttggg ctataataaa ctctgttagg aaaatttggt
     5161 ataattgata gtaagaatga caacatgtta gaaaggaaga gagcatgccc actttagcag
     5221 atgttgcaaa acgtgccaat gtttcaaaaa tgacggtctc tcgagtcatc aatcatccgg
     5281 aacaggtgac tgacgaattg aaagagttag ttttctcagc aatggctgaa ctggattacc
     5341 gtccaaatat tgctgcaaaa gcacttgtca cgaatcggtc gcagatcatc aaattattta
     5401 tactggaaga aatcgacaca actgagccct attacatgaa tttgctgatg ggaatcgcta
     5461 aacggatcgg taaagcgcat tattctctcc aattagttac aaatgatgcc ttcgacgtcg
     5521 gttcttgtga tggatacatc atcacaggtg tcagaaaaaa agacttcgat tggatcgacc
     5581 gtttggaaaa accagttgtc ttgtttgggg aaaattctga aggatatgat tatgtagaca
     5641 gcgataatga atatggtaca gcaaaagcaa cgcaatatgc cttagaaaga gattatcaaa
     5701 cgattattta tatcggtatc gaagtggacg agccttttga aatttcaagg gaaactggtt
     5761 atttatctgt gatgaaggaa gttgatcttc ctagtaaaat cttgcggttt ccaaatcatt
     5821 cgacagaagt agaaagatat atgacagaaa actgggcaga ttttacagat aaaacttgtt
     5881 ttgtatgtag ttcagatcgg ctagcactag gagcagtaag agggattata aatgctggag
     5941 gacacttgcc cgaagaattt ggtgtgattg gatttgatgg ggtattttta gaccaaatca
     6001 gttcacctaa attgacaacg atcaagcaag atattatacg tatgggtgaa gtatgtggag
     6061 aaatgttgtt aaaaaaaata gaagaaaaag gggcgagtca aggttatcgt cgtttttttc
     6121 ctgaactagt tatcagagag acaacgaatt aagtcttatt actatatata tatttatgaa
     6181 attcatcgct attttcttaa aataatcagc gcattgctac tgaaaacaat gaatctgtgc
     6241 tattattgag aaaagttcca tttttgagaa ggatggtttg aatgaaagaa atcagacgaa
     6301 aacatttccg ttttcctgcg tacgatgatg agttaggcgt aaaattaacc aacgacaaga
     6361 agcgagtatt atttgacgga caagaagatc ttttgactga acagaatcag tccccgtttg
     6421 aacagatgaa agaatttcat ttcacgaaca cgaacaccga gaaaatccgt cgcaagcgtc
     6481 gtgcaacaaa tggattgaag tcagctccgc gtaaggaaaa tttggaaaag cataaaggaa
     6541 aattaccgga ttataccggc cattaccaat cagaaatcac atcgacagga cgaaaaaaac
     6601 tttttggtga ttcctctcgt tctgtcagac aaagtgaaac aaggacagag tccaagcata
     6661 ctacacgaaa cgcatctgtt gacacgaagg tgcgggagcg ttcttatttt gttcctaaat
     6721 atgtacccgc atcgattatc ccagacccta gagaaagcaa aatttctgag gatcatttaa
     6781 tcgaatcgat gaaaaaaacg gatgactctt atctgttgtt cgatacagaa ccgacgccgt
     6841 atcaagaaaa aagaaatggt gagccgtctg tacgaacatt caatcgaaca aaaacgttag
     6901 aggaaacaaa gcagtcagaa gaaaaaaaat cgaaaaaaag ccatggtatc ttagaacgct
     6961 cattacaagg attgatcgat gatcaaggaa actctttggg agaaaatagc tattttaaat
     7021 aagtgatata caaatttata ttcggctaca gttggaaagg aaacgctgcg gcgtttcctt
     7081 attttatact ctatccgaaa gtttatatcg tatcctcaaa tttttttgag tttaagcgaa
     7141 ttttttctcc caataagtaa gaaaataatg gcaaaataaa aaaacaaact attttcgcag
     7201 atcgtttgca aaaatagttt atttttttat aaagaagatt ttactgaata atatcatttt
     7261 agtttacata atgatatttc tgtaaattaa ataaagtagt gaaaaataag aatcagtgct
     7321 ctaactgttc aagaggatca agtggttcaa atgtttctgt agtctgtttt gcttccaatt
     7381 ggggcaaaac agatccaatt tccagatact tcactttatt caatgtataa gatagaatag
     7441 aacagctaag taatgtatac agaaaatgtg tgagatccaa ataaagcaag gagacaacta
     7501 aaataccaaa agtaatcaag aaaagacttt tgccaatctt aatatgaaaa tatttttcaa
     7561 tggcttgata aatgatatct gttccgcctg tgcttccgcc gaatcgaaag acgagaccgc
     7621 aaccaatgcc agaaagaata cctgtcatca aactattgat cagtagacta tctacagcaa
     7681 aatgtatatg aaggctctcc cataaatgta aaaagaaaac tagcgcaaat gttccgacga
     7741 tcgtccggag aaaagcgtgt tttccaaaaa gaaacaatga gataagcaat aaaggtaaat
     7801 taaaaagcag tgaagtgaca gaaggagcga atccaaaaag attatgcaaa agcaaagcaa
     7861 taccagtaac cccaccatct gccagcttag atggaatatt gattcctgtc acagctaatc
     7921 cgtagattcc tgcgcctaaa atgattccgc acaattctgt aaataaattt aattttttca
     7981 tttctagcct cttttctttt gaaaattttc agcaacttct ttattatact gacttatact
     8041 cttacttcaa gaggaaaatg aaaaaaaatt atttttggct gtttcttttt atttatcaaa
     8101 taaggagtgg aaaaatgcga gcagcagact tattgacaat tcttacaaaa tcacccaaaa
     8161 aaacagagta tcaaggagtt tttttggctc aagaacaaaa actggtgcct gtgacaatgg
     8221 tccagataaa taaagcaaat caatttatct ttttatttga acacggcaaa gcaccacttg
     8281 ctatgaaaga catctttctt tttttgatga agaaccgtga gaaagctgtt tattatcagt
     8341 caaaagaaga actcattcct ctttatggag tgaaagagat agaaaataaa gtggtgatat
     8401 aagtaaaaat aagaaaacaa gacgtttatg atgtcaaaac aaccattttt tacatttttg
     8461 gcaaaaaagt gtcatgatac agtataatat tcgttgaaca ggccaataaa ggtgttctcc
     8521 cccacgagat ttctacttta ttggctgttt ctttttatta aaataaaagt tgtgtgtgta
     8581 aaaaagatat agctgattcc aagtgacaat ttcgtataga tttttatggt attcgtagtg
     8641 aaaaaacgag aaccaaaaag ttgatccgac tttgttgctt ggattctttt tttgtcagca
     8701 tatttagagg tatttttttc ttttttttgt tcgtgctgta aggttaaggc aagacaagtt
     8761 agttgggaaa aaggaaagaa atgtttatac ttaaagaata acagaaagga aggtcgcatg
     8821 aataatttta tacaaacatt aagtgaaaga aaaggcgagt tgctggaagc tgcatttcaa
     8881 catctgtcca tttctctcat ttctttgctg attgcagcac tgattgctat tcctttagcg
     8941 atttgggcag ccaatcataa aaaaatggca gaagtcttat tgcagatcac gggtgtgctc
     9001 caaacgattc cttcacttgc cttattaggt ttacttatcc catttgtagg gatcggaact
     9061 gttccagcat tgattgcact tgttatttac gctttgcttc cgattttcca aaacacgtat
     9121 attggtctag ctgaaattga cccgtctatt gaagaagcag cggtagcatt tggtatgtcc
     9181 agaatgagac gcttgctgaa agtagagctt cccatcgcat tacctgtcat tatctcaggt
     9241 atccgaactg ccttagtttt aatcatcggt acggctactt tggcagcatt gatcggagca
     9301 ggtggattag ggacatttat tttgttaggt attgaccgaa atacacctgt tttgacattg
     9361 attggtgcga tcagttctgc cttgcttgca attatattca gcggactgat tcgctggttg
     9421 cagcataaga aacctcgata ttccatcatt actttagcag cagtggtgtg tttatttggc
     9481 ggcgtttctt tatatcagtc tgccttgttt aaaaatgaaa ccatcacgat tgctggaaaa
     9541 ttaggagcgg aacctgaaat cctgatccaa atgtataaag aattgattga agacaacaca
     9601 aaaacagaag ttgaattaaa accgaatttt ggcaagacga gttttttgtt caatgcactg
     9661 gatcaaggtc aaatcgatat ctatcctgag ttcacaggta ctgtcctaga aagcttggtg
     9721 aaggtacctg aagcaatgaa aagtgctgat ttgacaaaag aaaaaacata tgacgaagca
     9781 gaagaattat tgaataagca attcgatatg acattattaa aaccaatggc ttaccaaaat
     9841 acgtatgcct tggcagtcaa gagatctttt gcgaacgaac atcaacttaa aaagatttcc
     9901 gatttggcag ctatctctga tcagatcaaa gcgggattca cactggaatt tattgatcgt
     9961 tcagatggat ataaaggaat ccaagaagca tatggcttga atttttcaag tgtacaaagc
    10021 atggagccaa gtttgcgtta ccaagcaatc aataatggag atgtcaatat tgtggatgcc
    10081 tattccacag atagccagct caaggagtac gatctagtga ctctagaaga tgatcgaggg
    10141 ttattcccag cttatcaagg cgcaccgctg atgaaaaaag attttgctaa agaacatcct
    10201 gaaatcgtta aagcactgaa taaattaagc ggaaaaataa cagaggatca aatgattgat
    10261 atgaattacc aagtcaatgt cgaaaagaaa aagccgacag aagtagcacg aatcttcctg
    10321 aaaaatgaag gattagtaaa ggaggaagat cagtgactaa attaatcgag ttcaaacatg
    10381 tgcaaaaaaa gtatgatgga aagtatgtca tcgatgacct aaatctggca atcaaaaaag
    10441 gagaaatctt tgttttggtt ggtccttccg gtagtggaaa aacaactact ttgaaaatga
    10501 tcaatggatt atctaaacca tcagcaggag acatctactt caaaggaaaa tcgttaaatg
    10561 aatataatct gcagaaaatg cggtggaata tgggatatgt gctgcagcaa atcgctttgt
    10621 tcccgaccat gacagtcaag caaaatattg aagtcatccc tgaaatgtta ggttgggaaa
    10681 agcagaaaag agcagaccgt gtagatgaac ttcttcaaaa agttggctta tctcctgata
    10741 tttatcgaga tcgtatgcct agagagttgt ctggaggaga acagcaacgg atcgggatca
    10801 tccgagctat tgctgcaagc ccagatgtga ttttgatgga cgagccattt agcgcattag
    10861 atccgatttc aagaaactca ttacaagaat tggttttgtc cttacacgaa gaattgggca
    10921 cgaccatcgt tttcgttacg cacaatatgg aagaagcaat caagctaggt gatcgtattg
    10981 catttatgaa ggatggagaa attatccaat gtgacacacc agagcagtta ttgatgaatc
    11041 ctaaaaacga ctacgttcgg catttctttg atgaaccgaa acagacgaaa gagtggcgag
    11101 tagaagattt agtcgtcaat ggatatttct taaatgaaat accagaaaat gctcgtcaac
    11161 aagtttgctt tgatacgcct atgaaagaag tatattcgct tctttcggtg tatccaagca
    11221 ttgtgatagt agaaaagcaa agaagtattg ggtcgctgac aagtaaagaa atctttgctt
    11281 ttttaagtcg agaggaggaa ggaaatgaaa acatatgatg caatcatcat cggttcaggt
    11341 gtgagcggct taagtgctgc ttatggattg aaagaagcag gtaaaacagt actagtggtc
    11401 gaagaagatc tatggggcgg gacttgtcct aaccgtggat gtgatcctaa aaaagtcttg
    11461 ttaagtgcag tagaggcacg taatcgggta aaacaattga gtggaaaagg ttttaacgag
    11521 atcccaactg ccaactggga agaacttcaa aaattcaaac gaacatttac ggatccagtc
    11581 ccagaaagcc gaaaaaaaca attagcagaa gctgaaatcg atcatctttc aggaaccgct
    11641 cgttttttgg atgattcatc tattgaagtc aacgaagaag tcttccatgc ggattactta
    11701 gttttggcaa ccgggcaaag acctacgatc ttaccagtgg agggaaagga atacttgaaa
    11761 actagtgcag actttttatc attgcctgta ctgcctaaag aaatcatttt tatcggtggt
    11821 ggttatattg cttttgagct agctacgatt gccaatgctg cagggagcaa agtaaccatt
    11881 gtccatcata atcaacggcc attaaaagaa tttgaggcgt ctttggtaga ggaagccgtc
    11941 catcaaatgg aagcatctgg cattcagttt gcttttggtg ttgaaaccca aaagatcatt
    12001 tcagaaggta ctcgctatcg tttagtcgga aaagagacgg aattagtagc agatatgatt
    12061 ttttgtgcga ctgggagaca gccgaataca gaatctttag cacttgagca agcgaatatc
    12121 gtctttgata aacacggaat tgcagtcaat gattatctgc aaacgagcaa tccaaaaatt
    12181 tttgcgtgtg gagatattgt ttcaaggaaa accccaaaat tgacaccagt cgccactttt
    12241 gaaggaaatt atgtagctaa gcggataacg gatgcaacaa gcgaacctat caaatatccg
    12301 attataccta cgatcgtcta cgccagtcct aaacttgctg aagtaggagt tacaaaaagt
    12361 cacgcctctt cgtcagatca agtggtagag atggatctta ccagttggtt cacctatcat
    12421 cgtgtcaatg aaccagttgc taaggctgaa ttgacctttg accagcaaaa ttatttgatt
    12481 ggtgctgcag tgattagcga gcaagcagac gaattgatcg atgatttgac actcgtcatc
    12541 aatcaaaaat tgactaaaaa agagttggat agctatatca tggggtatcc gacattggca
    12601 agtgatctct cctatttatt gaaataatca gcatgataac taaagcgctt gttgcttttt
    12661 ccttcttgtg ctacacttta aaaaatgaat acctgtttct ggggatgtcg tagacgacat
    12721 gagagtaagt tgtgaactta gacccattga acctgtcagt taatactggt gaagggagaa
    12781 acatagcaga cgcttgaagc gggttactat gtcgtgccca tttcaagcgt tttttgttat
    12841 tcatattaga agaaaggagg taacaaaatg aagaaaaatc tttatccagc agtcagtatg
    12901 gcattcattt taattatttg gcagattgtt gtcatagttt ggaaaatacc tgcctttatt
    12961 ttacccggac ctgctgctgt attttctgca ttagcggcag atgcacaagt attactttcc
    13021 cattctgtca cgacattaaa tgaagcagtg cttggtttgg taatagctag cgttctttct
    13081 tttattactg ccttggcaat ggattattgg aagtttttag aaatgtcttt ttatccgtta
    13141 ctagtgattt cacaaatgtt gccaatcatg gtgttagggc cattacttac cctatggttt
    13201 ggtttcggta tgcttccaaa agtgatcctt gttgtcttga taagctattt tccgatcgta
    13261 gtttcttttt cggatacatt gaggaaagta tcaaaagagc aaatcatctt tttgaaaacg
    13321 atgggggcag atacagggaa gatttatcga atatataaaa taccagcggg tttatccggc
    13381 ttcttttccg gtttaaaagt ggctgctact tattgtgtca gcggagcagt cgttggagaa
    13441 tggctaagcg ctcaagcagg cttagggtat tacatgatac gcgtaaaaaa cagctaccag
    13501 atcgataaag tttttgctgc gatcatttgt gtcattctat tgagtttatt gctgaatggc
    13561 gcttgttcat tattgaaaaa agggtattat cgtatgcttt atatttttta agagaagggg
    13621 atcaaatgaa aaaattatgg cttttattac cgctactgtt gcttttatcc gcttgtggca
    13681 ctgcaaaaga aacaagtagc aaacaagaaa taaaggattt gaaaaaagtg acacttgttt
    13741 tggattatgt accgaatact aatcatactg gcatctacct agcaaaagaa aaaggctatt
    13801 ataaagaggc tggtttaaat gtccaaatca tcgaaccagg agataattcg acaagtattg
    13861 gtctagtagg tgcggacaaa gcacaatttg gtgtaagtta tcaagaagat gtcacttatg
    13921 ctcacgcaga tggacagaat ataccagtca aagcaattgc tacagtgatc aaacataata
    13981 cttctggatt cgccacccta tcagacagta acatccattc accaaaagat tttgaaggaa
    14041 aaacatatgc aggttggcaa agtccaagtg aagaagctgt cttaaaagct gtgatggaaa
    14101 aagacggcgg ggacttttca aaactgacaa tggtaggaag caatggagaa gggccagaaa
    14161 gcttaggaaa atcctcagat atccaatggt actttgaagg ttgggatatg atcaaagcaa
    14221 aagaagcggg tattgaacta aactatatcc cattgaaaga attagatgaa cggttagatt
    14281 actatactcc ggtaattatt acgaatgatc aactgatcaa aagtgatcca gaattggtac
    14341 agtcttttat ggatgccacg aaaaaaggct atcaggaagc tattaaggat ccaaatgaca
    14401 gtgctaaact gttgcaaaaa tatgcaaaag aaaatgatcg gacgttttta gaagaatcac
    14461 aagcattctt gtcaaaaaat tatacggatg atcctgagaa ttggggattg atggaagaaa
    14521 aagtttggcg caattataca gcttttatgc aggaaaacgg attgatcaag caagatgtac
    14581 cttcgcaaga actattcact aatcagttta taaaatagga gtgatagata tgtcaatcca
    14641 aatcagccac ttgaatgtag tcattgaaaa cctgaaaata ttagaagaca tccatttcac
    14701 tatccctaaa gataccttta catcgattgt cgcgccaagc ggagccggta agtcaacatt
    14761 gctgaaaact ttaacaggag tccagcctat gacttctgga acgatcaaag tagacgatca
    14821 aaaagtaact ggtctccata cagcatttag ttatatgccg caagaagata tgttacttcc
    14881 ttggctgagc gtctatcaaa acgttacgtt atatcaaaaa atcaatcatc taacaatcaa
    14941 tgaagaacaa gtaagggatt atctggatat ctttgggtta aacggctatg aacatttttt
    15001 gccggaacaa ttatctggcg ggatgaagca gcgaacggcc ttgttacgaa caatcatgaa
    15061 tccttcttct tacttgcttc ttgatgaacc atttggagcg ttagatgcta tgaccagagg
    15121 gcagatgcaa gactggatgt tgaagctgcc taaaaaagca aagcggacta ctttacttgt
    15181 gacgcatgat atcgaagaag caatttattt atcagacagg attcttgtgc tttccgcccg
    15241 tccggcacat gtgattgctg agattcaagt gcctgaaaaa cgacggtcac gtgaatggct
    15301 actgcagcaa tcagagttga agcaaacgat ttatcagtta ttggcaggtg aattagatgt
    15361 taagtgatgc tcattgtcat ttagatggga gtcagagttt agcactcctt cagcaggaac
    15421 acacgatttt gacgatcatc aattgtgatt cgccagaaga atggaaggaa aacagacagc
    15481 ttgcagcaag taagactcaa gccttaagtt acggtatcca tccttggaaa gcagatagct
    15541 atacgtttga acaagttgag ccttttttga aaaaagcaag gatcatcggt gaaatagggt
    15601 tagataatat ttggacgaat gtaccgatga ctacacaaaa aaaagtattt gagcgacagc
    15661 ttgcttttgc agccataaat gagaaacctg ttgtactgca cacaaaagga tgcgaaaaag
    15721 agatcttaaa ttatatccaa gcttacccta atcactattt gatccattgg tattcttctt
    15781 tggactatca acaagactat attgatttag gctgttattt cagcattggt cttgatttga
    15841 aaaagaatcc agcagtctgg caactagcta aagcagtccc aatcgaccac ttacttattg
    15901 aaacggatgg actacatgca gcagagtggg ctttaggggg gattttttca gaaaaagatt
    15961 atgtctcact gcttgaagaa cagttaaaga caatcgctga gatcaaagag ctatcagttg
    16021 aagaagttaa aaaaaatact tatcaaaact tggaacgttt cattagatta ggtaaataaa
    16081 aaagagatgt gacacaacta gtgtcgcacc tctttttggc tttctttgta taaaagtaaa
    16141 ctgtttgatt aataaatgaa tcttagtacc aaccgtttgc ttcccagaat gctttcgccg
    16201 cttcccaaga accataacgt gaagtaacat attgttctgc tactctttct tggtttgcag
    16261 cagagtagtc accatttaag taagaagaat ctaattggta acgtccgatg taacgtccgt
    16321 tcgttgctgt gtaagaaccg cttgattctt tttgtgcgat ccattctttt gcagagcttg
    16381 tgcttgctgg agtagcagca gtttctgtta cagcaggagc aggttgtgtt tcagcaacag
    16441 gcgctgcttg tttttccgtt tgaaccgttt cttgtacaac aggttgttca gcttggactg
    16501 gtgcttcttg aacggctgct tgaggttcag cagctgcttg tgcagagcct tctgttggga
    16561 ttgttaattg ttgtcctgag taaatcaggt taatatcaga aattgaattt gattctgcaa
    16621 tggcattgat caaggagtta tcaccaacgt atttttgaga aatcgttgat agagtatcac
    16681 ctgattgaac ggtataagct tcgtccgcat gagcagtcgt tcccatgaag aatgcagcgc
    16741 cggcagccaa agttgttcca aaaagtaaag ttttaagtga tgtcatgaaa aatctccttt
    16801 attcgttaaa aagttttatt tgttgtgttc aacgaggaat actttaacac gtgaaaatat
    16861 ttcataggta aaagaagcat gacaatttat gacaaatccg cgttttttgt aataaataca
    16921 gtaatctatg aaatgaaaag ggttacgttt aataaaactt gcttttttgt ttttgttcac
    16981 cataaatatt tatttaagaa agtattaacc tgcatttttc tctataaaaa agatttttta
    17041 aatagataaa aaaggaggat taaatgagta tttgatagta tttatattaa aattacatat
    17101 gtaatttaaa aacgcttgac attgtgtttg agatcactta cttttagcat gtaaaagtat
    17161 tggattaagg agctatgcaa atgaaaaaga aatttattac tggtgggatt gtcgttgcat
    17221 ttctatgtct aattggctac acaggcacgc ggtacgcaaa tgaaattatt ttgtggggtg
    17281 gagagacaga tattgataca atcaatgaga atctgacaca gcttgatcat acattgacag
    17341 ataaagagca agcgattaca gatcttaccg ctcgactagc acaaaaagaa cagacattgc
    17401 aagattcaaa ggcagatgtc caagaatacc aaacaaaagt aaatgaatta gaaaaccaaa
    17461 aaaatcaatc ggcagcagaa aaacaaaact tagaaagtca attagtcagc aaggatgcac
    17521 aactacaaag caaacaaaat gaaatcaatg tcaaactaga tgagatcaac caaaaaaatg
    17581 atgaaatcaa tcatctgaat gcagattgga cagcaagact acaagaagca caagcaaaac
    17641 tttcagaatt acaaaatact gtaaatggtt tgaatcaaca aatcgcaaca ctgacacaag
    17701 aaaaaacaaa tgtagaaatc agttaacaga tacacaaaag aaattagacg aagcaactgc
    17761 tgaaaactca agcttgaaac aatatattca aaagttagaa aaagcaaaag atgaagtgga
    17821 agatacagct gataaatctc agcaaattgt tgaagaacat ttgcgtaaat aatctttctt
    17881 aacaagtaaa cctaaattag taaagaagca acagtaatct atactgctgc ctcttttttt
    17941 tagtacatgt ccttcatctt cgcaggaagt gccaatatga ttttaagaaa ggtaatagac
    18001 aaaaagtcaa aaagataaaa cagagtttct tctttacaaa gggaatgtat gttcgtataa
    18061 tggtattgag gtgaaggtta tggagaaaaa atatggaaca gtagataaga tccgtacttt
    18121 gcaagttagt gccagaccgt tgatccgttt cagtttaaat ggggtcaatt gtttgattgc
    18181 ttcccatagt ttgaacttcc tagctgaagt agacgaaggg atgaagttag tcgttacagg
    18241 ttattataat aatagaaagc aatttgttgt gagatcctat catgtgttag ggaaacccaa
    18301 aattgtggta gaatatgaga aaagtctata cccaagaaaa aaggtccgac aagatgaaag
    18361 aataaactag aaaacaagcg gctgagacaa aaatgtccta gccgcttgtt ttggaaagat
    18421 ggatgcagac gatgggattt gaacccacac gagcatacgc tcacaagaac ctcaatctat
    18481 atataactac atgtatttaa gtatttgtga tgaatcacta aactttccaa actcatttga
    18541 gggatattgc ttaaagattg aacagatcaa ttcgaagccc tattagtatt cgctcaatgg
    18601 ggggatcaga ggccataaaa agagactagt cattactagt ctcaggtagt ggaattgtag
    18661 gattttaaac gttcctggag agtaccggta tgtaactcga agaggtgatt atcatagtcg
    18721 taaaagtaaa ttgaagaagc ctcgcctttc acacgagaac gtcctggttc gacagttaaa
    18781 cctaacattt ggatttttga gacaaacgaa tctatatctt gttcgtcaat tttaaaagca
    18841 atatgattgt atgtctttgg cagtttattc gacgaattct gcattactgc aatccatagg
    18901 tccttaacta agaaaaattt ttctggatat aatgaaaatt tttttgttga ctatcgtaga
    18961 tttcttttgc atcaaataag gtatttaaca attaagtatt taaaagataa cttttaagtt
    19021 atctttaagt aatgcttaaa gctatgttat actctattag gaaatataag aaggagtata
    19081 aaaatgaaca aacataatag aaaaataacg aataacaaag aactgatctt gttactaata
    19141 gggatttggg gtatcttttt actctttaat atgtatatgc cgactatgag agcggatgac
    19201 gtagtttatg caagtagatt agatgaactt ggctatttgg gtgcttcgat agaacattat
    19261 aagacatgga gttcaagaat tattatagaa ctattcttaa tgttcttttc taaacacttt
    19321 atgctatgga aattattaaa ttctacgatt atgttaggaa ttgtagtatt actttgcaag
    19381 tat
//
LOCUS       DAFEOM010000048        19336 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.049, whole genome shotgun sequence.
ACCESSION   DAFEOM010000048
VERSION     DAFEOM010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.049"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            148..585
                     /locus_tag="LP524_001888"
     CDS             148..585
                     /locus_tag="LP524_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234922.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            680..1564
                     /locus_tag="LP524_001889"
     CDS             680..1564
                     /locus_tag="LP524_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBL2234923.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1788..4955
                     /locus_tag="LP524_001890"
     CDS             1788..4955
                     /locus_tag="LP524_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234924.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4975..5520
                     /locus_tag="LP524_001891"
     CDS             4975..5520
                     /locus_tag="LP524_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HBL2234925.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            5548..6003
                     /locus_tag="LP524_001892"
     CDS             5548..6003
                     /locus_tag="LP524_001892"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HBL2234926.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            6000..6758
                     /locus_tag="LP524_001893"
     CDS             6000..6758
                     /locus_tag="LP524_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBL2234927.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6844..7584)
                     /locus_tag="LP524_001894"
     CDS             complement(6844..7584)
                     /locus_tag="LP524_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2234928.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7645..8898
                     /locus_tag="LP524_001895"
     CDS             7645..8898
                     /locus_tag="LP524_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBL2234929.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9220..9447
                     /locus_tag="LP524_001896"
     CDS             9220..9447
                     /locus_tag="LP524_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBL2234930.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9570..9896
                     /locus_tag="LP524_001897"
     CDS             9570..9896
                     /locus_tag="LP524_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBL2234931.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9964..10677
                     /locus_tag="LP524_001898"
     CDS             9964..10677
                     /locus_tag="LP524_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBL2234932.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            10987..11838
                     /locus_tag="LP524_001899"
     CDS             10987..11838
                     /locus_tag="LP524_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL2234933.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            11959..13824
                     /locus_tag="LP524_001900"
     CDS             11959..13824
                     /locus_tag="LP524_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL2234934.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            13872..15281
                     /locus_tag="LP524_001901"
     CDS             13872..15281
                     /locus_tag="LP524_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBL2234935.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            15389..16090
                     /locus_tag="LP524_001902"
     CDS             15389..16090
                     /locus_tag="LP524_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL2234936.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            16249..16728
                     /locus_tag="LP524_001903"
                     /pseudo
     CDS             16249..16728
                     /locus_tag="LP524_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            17576..18151
                     /locus_tag="LP524_001904"
     CDS             17576..18151
                     /locus_tag="LP524_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HBL2234937.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            18151..18591
                     /locus_tag="LP524_001905"
     CDS             18151..18591
                     /locus_tag="LP524_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBL2234938.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            18607..19308
                     /locus_tag="LP524_001906"
     CDS             18607..19308
                     /locus_tag="LP524_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBL2234939.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         6483 a         3217 c         4139 g         5497 t
ORIGIN      
        1 taaatcaaca gtagtttaat tactacttga tttagttctt tttattttaa aaattttttt
       61 tacttagaat ccagcgaccc tagatatttt tctgtataat cttaaataaa gaaaacaata
      121 tcaaaaaatg cacgtagaag tagataaatg gagtggtttt gcaaatttct tttatttcta
      181 gaagttattc acgtatacgg atttgttttt ctcacaaata aaaaggcagg aagtacaatg
      241 gatcatttgt atataaaata tattcatcaa ttttccgttt tggtgttagg cacactaatt
      301 atagcgggga ttgtcgcatt tattattcaa ggccgtgaca tattaacagt acatcaagag
      361 tttttcagtt atgcatttgg tattcctctg atcgctataa ggtctgataa caaaaagtgg
      421 aagaaacgct attacattct cttgctattt ttgtgttttt atctgccacc taagattttt
      481 actttattag gtatacaatg cctatcattt tcttttattt cctatatcat tgttttaatt
      541 ttcctcagtt tcatagtatg gatgttttat cttgtcaaaa aataaatgaa tgtttcatga
      601 acagtacttc atcacagcct aatttttttc tttatgctat cgttatatta aagaaatcaa
      661 tcagaaggga aagaaagtga tgaaaaaagt tttattagtg gtaaacccca gttcaggcgg
      721 tgaacaagca aaggaatttg agcaattagc gatagcaaaa ttggaatcgg tgtttgatga
      781 agtggtcgtc ttgcatacaa aaaaagcagg ggatgcaaaa aactttactc gcgaagcggc
      841 tacggaaggg tatcatagtg tgtttgtcat gggcggagat ggaacagtca acgaaggaat
      901 cagtggaatc gctgaacaag aacatcggcc aaatttcgga tttttcccat tgggtacggt
      961 gaatgacttg gctcgagcat tagggatacc gctagaacct gaggaagcga tcaatcattt
     1021 ttcgattgaa tcagtcaagc cattggatat tggaaaaata aatgacgact attttatgaa
     1081 tgtcgtcgca attggctcta tccctgaatc aatcaatgat gtagatgcgg agaagaagac
     1141 gaaatttgga aaactagctt atttcatgtc aggaatcaaa cagttagctt ccacacaatc
     1201 ctactcgttt cacgtagaag ttgatggaaa gaaggaagaa attgagagca gtaccttgct
     1261 gataggacta actaactcag tcggcggttt tgaaacatta cttccaaatg caaaagtgga
     1321 tgatggtaag ctccatttgg tttatttaaa agattcttct ttgatcgata cgcttaaaac
     1381 attgccagac ctattaaaag gagtagatga atctacgaaa aatctagcct accttacttg
     1441 tgagcaatta gttgtgtcat tagctgatga ggaagaattg acgacgaatg ttgatggaga
     1501 tgaaggggat aaattacctg tgacaatcaa agtattgccc tcacatttac aggtttattg
     1561 ttaaaatgga ggttgtgacc gaggagttgg cgggagaaca ccgcttgttc gggataaggg
     1621 actgtgacaa gacttttgtc acagcctttt ttttgtacaa aaaagaaatt tacacctaat
     1681 agaaaaattt ttccataaaa atacatattt taaatatgaa agtacaattg gaaatagatt
     1741 aaaacgataa aataaaattg tcaaaacgtc agagaggatg gtggagaatg aatcaatgtg
     1801 taaaaaagct gattgcgtcg attttgtttg tcttccctct cttgatggtg acatccgttt
     1861 ttgccgatgt agagaaaaca aacgaatcaa cgcaatcaga gaatcaaagt tttgacaaga
     1921 ctactttttc agaagaatcg attacttctg aaactacaga atctcaaagt gaggcttcta
     1981 ctaataaaag gcaagaagaa aataagacaa gtgagacctc agaagtagct gaacaaacgt
     2041 ctgatagcag tgaagcggca ctggcaagag ctgtttcagc ggatgggtac agcgaagctg
     2101 ccacaacaga agaactggcg cagcttctcg cagacgaatc agtaactaag atccgactga
     2161 ttcagccttt gactttagac agggaattag agatcaaacg agatatcgtt attgattttg
     2221 gtggattcgc tcacaatttc ggcacacacc atatttatat caatgagata cctcagtcga
     2281 tcgagttcca gaattttaaa ggaacagctg cacatccagg tggattgaca ccaaatatcg
     2341 atggaaatgc cattatcatt gcttatatga ccgatttttg gggaagcaga tatcatttta
     2401 cgggagaaat aaaatttacc gggacacttg atttgtatga tgggtccaaa ttaggactga
     2461 tttacgctcc tcgtgcaact gtcacattag atggtgtttc cggtgtgttg gatgtccaac
     2521 cagtgaagga aggactgaat gccgctccgg gtaaagccta tttttgtaga agctatcaat
     2581 tgaatgtgat caatggtagt caactttatg gaccgtatct agggaaattc tacggttttc
     2641 tagattcagg agatgagact ggtagtggaa aaacagcgcc aggaatccat atcttaagcg
     2701 gttcaaaggt ctctcttgat tatgatcggg cagatggcgt tagcgatgaa ggagaggcag
     2761 tcgatacgct acctggtaat gtcatgtttg aagtcagtgg ttcgggaagc gaattttcgg
     2821 taaatacaaa gatcaatatt accaatgaca acaatcgcgg aatcatccaa atgagaggtt
     2881 caggatcgaa agtcactgta tcgaatggtg ggaaaatcac catcaataca gcaactacgg
     2941 gcggatttcg cttgcaagga gattctagtc aaatccatgt atcaagtggt ggacaaatct
     3001 cggtcaaaat ggctggagat gacgatcaac ccgggaacaa tgggatgcgt tttgtaggaa
     3061 aaggcctttc tttgatcgta gaaggagccg gatcagttat cgacatcgat aagcagagtg
     3121 ggcgttccag cgcagttcgt tttgagaatg gtacgcaaaa gctgacagta accaatggag
     3181 gctcattgag ggtaagaaat acaggtgatg gcaaatctta cgtgaacaga ctcaatcaag
     3241 gaaatcaagc gatccaattt tatgacgctt ctggttttct agaaagtcca ggttcagctg
     3301 attttacagt cactggagaa aaatcaaata tcgatatacg cgcagattcc ggagcaactg
     3361 tagatacaac tggagatatt gatttaaatt ttacggcagg tgaaaaaact tatttagtca
     3421 tggtcgggaa aacaggggaa gataagaccg gcatcatcag tggggatgtg ttgaatgtgg
     3481 acttagaatc tcctacctat tttgatttcc aaaacaaacg gattggaagc gagaccaatg
     3541 gcataggcgg ctgggtattc caaggaaatg gagattcttc tttaaagatc gcccattcag
     3601 aaatcgcttt atggcgaaaa acaggcgcaa cattcgataa ctttgaaaaa gacccggatt
     3661 atttttctga tatgacagac ttatcgatca atgggaccga cttaggcaat ttcgtatcaa
     3721 gccctagtgc aggtttaaca aatgaatttg ctcagacagg tgccggaatg aaaaattata
     3781 atcgaatcag tgccaataat cagctcccga tgattgaatc cttaagagtg ccaacgaatg
     3841 cagataaaaa aatctatggg catgtcgttg ttcccgaagg tgtcgaggca aagccaagag
     3901 atgcttggac aggcgaagta gaagtgactt tacgtattac ttacgcagat acgacgaaat
     3961 catctgtaga aattactgct ctcacgcaag gacaaacaga tgaaaatgaa ggggcaggat
     4021 ataacccatg gggagaaggc gaacaaggcg gcttgttcga agttcaagta cccaatgatg
     4081 agagattaaa aacggaggat caagtgcaag ttgttgctgc taaaaaagta agaggtggcc
     4141 aagaagtgac agaactggac tctgccaaaa cgacagtcga tgtgattcct cctgaacctg
     4201 ctaatttaag taccacattg atcaatgcag cttctcgcac gatcacaggt acaggtgaag
     4261 caggagcctt ggtttcattg aaaaaaaatc aagctgtttt gtcagaaaca actattgatg
     4321 atacagggga atttgttctt tctatcccag aaaatcaact ttttgcagga gatgtattag
     4381 aaattgtatt aaacgaccaa gcaggagaag ctactgctaa gggtgtgcag aacaagccaa
     4441 gtaccaacga tgaaattggg aatcacaatc caagtgatac gccagttgtt tatcatgatg
     4501 ccccgccttt tgcacctgct acgaagatcg tagtagaagg tggtctctca tttcttgccc
     4561 cttccaaact cgattttggt cagatcaaag caactggttt gacacaagaa gcatttggcc
     4621 aaatggatat acaggaaaaa ttatggattt atgatcagcg agaaacgaaa aatccgtgga
     4681 tcttaacgat aaaattatct cagcctttca caaatgatgc aggattttct atgcaggatg
     4741 tcctatattt caagaaagga gaggagtatc aattagtgaa tgaagaagaa ctggaaattg
     4801 tcagaggaca aaattcaacg aatgtgcaag cagattattc agaatattta aataacggtc
     4861 gggcgtttaa acttgtctta accaaagaaa atcagatagc tggtaattac tcagcagaga
     4921 taacatggac attagcagat gcgccgacag aataaagatc aaggaggatg attgatgaat
     4981 cgaatatgga ataaaagtca tcgaaacaac tgctctttac ttatgatcct tctccttgga
     5041 ttcatccttt ggcagacacc tgtccatgcc tccgaattca attttgcggt agattttgaa
     5101 ttaccggcta atcagattga taagaacaaa acatatataa atgtaaggat ggagcctggg
     5161 aaaacacagg aaatccccta cacggtcttt aaccgaacag ataaatcagt aacagttgag
     5221 acattgatcc actccgctac gacgaattca aacggtgtga tcgaatatgg agaatcaaaa
     5281 agtaaatttg atgaaacatt aagttatgat atgaaaaact tagcaaaaac agtgggagaa
     5341 ataactgttc ctcctcaaag tgcggtcact gagacacttt cactgaaagc gccagaaaaa
     5401 gagctggctg gtgtggtcgc aggagggatc acgtttcaag aaaaaaaaga gaacgtttct
     5461 aacaaaagta cagttggtgt cgtcaatcag ttttctcaag tgaaagcaat cttgatttga
     5521 aatagcgaaa aagagtcttc cccagatttg aaattaaaaa aagcttatgc ggatcaaagc
     5581 gatggaagaa atgtgattaa ggcgaatctt caaaatccaa atgcttctta tatgttcggc
     5641 ttgactacat cggttgaaat ctacaaaaaa gaaacaaaag agccttacct ttcaataaaa
     5701 aaagaaaaaa tagatgtagc acccaataca aattttgatt tacttgtccc attagaagga
     5761 aaaaaattag ctccgggaca ttatcaagcg gttgtgaagg gtgcttggaa agaaaaacgc
     5821 tggaatctcc ataccgattt tgaaataact gatgatgcag cggaccaatt gaatgaaaag
     5881 gatattgatt taaaaaatca gccagaaaca tttgactggc gatggctgat acttgttgga
     5941 gtcattctta ttggcttatt tgctggatta tttattttaa aagaaagaag gaaaaagaaa
     6001 tgaaaaaaac aattgcatta agtaccgttc tagctggaag tctattatta tgtacacaaa
     6061 gtgtttttgc tgagaataag agctcagcca ctacagaagg aacgatttct gtgaccaaac
     6121 cagatgctgc aaatcctgaa gacgttgttc cagttcctcc tgttgatcca aatgatcctg
     6181 atactccttt tacacctgac gaaccaaatc agggaacatc cggattactg acgattgacc
     6241 aagctccaga tcttgatttt ggaacaatca agttaggtac agcaaaaaca gtatatgctg
     6301 cattacaaag cggaacggac agtactggag cacaaacaca agtacagaat ttcgtacaag
     6361 taacggataa aagcggaaat tatgccggtt ggaaattatc agtcaaacga acagaattta
     6421 ttggtacaac atctgatacg aataaactaa ctggttcaaa ggtgaaattc aaaaacgccg
     6481 tgattcgaaa tagcgcaact aatagcagtg gaatgccatc taccgtttct ggaactggcg
     6541 ctgaaggaat cgaaatccca gcagatcagg cagtagatct agtaactgca ggagcaaatg
     6601 aagggattgg cacatggatt tattcattag gagagaatgt ggaacaaggg agacaatcag
     6661 tggaattatt tgttcctgta tccaattatg ctgtagacaa ctacgtttca acattcacat
     6721 ggtcgttaac agatgctcct acagaagagg aagcgtaaaa tcatatcaac gatttcaggg
     6781 agtggagccg attcaaggcc cactccctga aatctgctat gcttacgtct cagctcttat
     6841 tcatcaactc gaagcagttt tttggaagtc atggaaacgt tcctttgtct tttcaaagat
     6901 ctttcgacca acgagtaagg agtcaccgtt gaaaaattct aacacataat gagcagaatc
     6961 aattttctgc aattgcgata agttgataca gtaagatttt aaaagtaaaa agtaagcata
     7021 cttttctaac tctgtcttca attgttttaa agaagtattc actaatttat cttctgtagt
     7081 tgttttgacc ataacttggt ttttcaaacc tttgatactt tcaatatata aaatttcccg
     7141 aggatacagg acgagacttc cacctttttg atcaataaag attttatcga catttacgat
     7201 ctgctgttct ttccaattga tctgttctag cacttgacga atttgttcag ataaattcct
     7261 gtcttttctg atatatccaa tcggaaggat tttccgattg atgatctcta ctgcaaaatt
     7321 cgtataagcg gtgaaaaaaa caatattact atgtgcattt tttttacgta tttgttcggc
     7381 gacatcaata cctgtgagtg attgcttcaa gtcgatatcg ataaagaaaa catcgtgatt
     7441 atcaatagtg cccaactgga tcaactgatt gatttgagag atagaagtga gctgcacgat
     7501 attctcgata tgatgattga ccaattctct gatgatccat tcgctgtgaa actggttatc
     7561 ttctatgata aaatactttt tcatatgcag gctcctatta aaaaaatatg tgtacatttt
     7621 gaaacaaaac aatggaggaa taagatggtc gcaacagtga cactttattt gacctttact
     7681 ttgattttag atttaggtat ctatttattc ttttcgaaac aactgctagt aaaaaacagc
     7741 tttggttgta tagctttgat ggtggtgaca cttttacttc ctatccctaa agtgagcttt
     7801 ccttacctag ttggcttatt attgatgttt cttttttatt ttactacgaa aaacctgttt
     7861 atcagctgtt tgatcagtaa tctttttttt gctatcactg gactcagctt ttttctggtt
     7921 tttgatctga tggattattt aggaaagaga catcttggaa cagcaagttt ttttgcacta
     7981 cttatcgcat ttattttcta tctattagta aaaagtatag acaaacagac gagatttctc
     8041 aacttgcttt ttacttatcc aactgaacac tattttggag gtatttgtat tttattaagc
     8101 tggggcgtga tttacttggt attgggaagt gttcacttcc gctcatctaa ttatctgctt
     8161 ctatctcttt tgtcgatagt catcagtttt ttttctttgt ttttttccat tatgttgatc
     8221 agtttccaag taaaagaaag acaacatcag gaatcgcttc aactgtatca aataaatgaa
     8281 gaatattacc atcatctaga ggaattcaaa catgattata aaagtctact gttttcatta
     8341 aaaattactc ttcaggaaaa taaaaaagaa acgttggaat ttttagaaat attagaagat
     8401 cattctgcag ctatttttga aaaaccgcaa atacaacagc taacaaatat tgtatcccca
     8461 gctgtcaggg gtgtcttttt aaaatttcta gaaaaagcag aatcggagaa aatcccgatc
     8521 caagtagtta ttcctgtcga agtgaaaaca atcccgattg atctggttgt ttttattcgc
     8581 tgtttatcta tttttatttc taacgcattc gatcatcgtg tagccgatca gagtccgatt
     8641 acgatcaatc tgctggaaga aaaagaggga tcacgtttta gtatagcgaa caaggcaaat
     8701 ccaacgaatt tgacgctatc ggagatggtc aaaagaggaa aaacctctaa aaaaaatggc
     8761 gggttaggtc tatacagtgc gaaaaaaatg ttggatgttt acgaaaatgc agaactaaaa
     8821 atcgaatttt caaaacagaa tcactatttt tttgtagaaa tgtatctggc atctagaaag
     8881 agcaaatcag cctactgact tactgtcaaa ggataagttt gaggaaagtc tacacggaga
     8941 tagagacaac aacacagcat attcaaaaga aacagaagcg tgatgaccgt tgtcaccctt
     9001 ctgtttcttt tgctatgtag ttaaacaaag tagttgacac ttccgctact tagtattagt
     9061 acgaaagtat acttcatgat actaagtaga taagaagtga aaagcggaac ccacgatcgt
     9121 caaaatgcta actgctttgc ttacaccaga taaagggaag agcaatatcg cttattttag
     9181 gcgtggagaa gcaagcaaat ccctacgatc acaaaaaaaa tgctgaccca gcagttaaga
     9241 gagttagaac aagatgaaag tatccatcga aaagcctata accaagtccc gcctaaagta
     9301 gagtactcgc tcaccgaaga aggaaaatca ttaagagaga ttctagtagc gttgtctgtt
     9361 tgggaagaag aacgtatcga aaaacaaaaa gcctcaaggg aagaagacac gctactgcat
     9421 acacatggtg gctacctaaa ttattgattt tctatgacat ttattttaaa taaaagaaaa
     9481 caggagtttt tttaaaagca tcatctattt tttatgtaaa aaaatggtat gattaatcaa
     9541 gtaagaagtg attggaagaa ggcaagcaaa tgaatagtat ggaaaaagtc aaccagttta
     9601 gagatgaacg taattggcga caatttcata acgaaaaaga tttggctatc tctatttctt
     9661 tggaagcatc tgaattgtta gaactttttc aatggaaaac gccggaagaa gtaaaagaaa
     9721 cacaattaga acgaatcaaa gaagaactgg cagatgtatt gatctattca tacatgatgg
     9781 cagacaacct tcacctggat ctagatgaga tcattgaaga aaaattgatc aaaaataatt
     9841 tgaaatatcc tgtagaaaaa agcgcaggtg tacgaaaaaa atacacagag ttgtagaaat
     9901 gtgtcattcg gctgttggaa agagcaaaag aaaaacagta aaatgagtct ggagggagag
     9961 aaaatgaaaa aaaaaggaaa agcaaaaaga tggctagtca atatcctctt gttcctcttg
    10021 ttattagtag gactagcttt gattttcaat gaacagatca aggattattt tgtcagagag
    10081 acaggagata aatatgctat agcgaatgta acaaaagaag atctgaaaaa gaataacgat
    10141 aaagatgtca gttttgactt tgatgcggta gaaccaatga cgacagaagg ggtcatgagg
    10201 tcgcaaatga gaggaacaga tctaccagtg attgctagta tcgcagtccc ttcagtttca
    10261 atcaatttac cggttttcaa aggcttggat aatacgtcac tactttacgg agcaggaaca
    10321 ttatctccag atcaagaaat gggaaaagga aattatgcct tagcaagcca tcgtgcaacg
    10381 aaccctgaat tgctgtttac accgctagaa aatttagaga tgggtgccaa gatctatttg
    10441 acagatttag aaaatgtgta tacatacaaa acatttttta aagaaaaagt tgctcctaca
    10501 gatacccaat tactgaatga agtcgaggga aaagaaattg tcacactaat tacttgtggc
    10561 gacatggacg ccgtcacgcg actagttgta caaggagaac tggaaagtgt tacatcgata
    10621 aaagatgcga cagatgatat gagatctgct ttcaatctag agacaaaaac attttaggat
    10681 actgggacat aagtcgttac agattaaaaa cagccaaagg atatgaatcg ataaggaaaa
    10741 accaatttgc tgaaaaaaag agaaacgcaa agaggtgcat ctctgatttg gttgttttct
    10801 agatagactt gtgttccagt ttttttattt tttatatgaa aaggcttgca taaataaaaa
    10861 ttcgatggta tgatgatttt gaataagaaa tggattgtta ctgtacggca ggcaaaacca
    10921 gaattcagct aagaaacagt aggcgaattc taggtttttt tatttttctt gagagggcga
    10981 agcaaaatga aaattgataa aattttgaat aacaatgtcg tcatttcgaa aaatggattc
    11041 ggggaagaag tcgtttgtat gggaaaagga ctagcttttc aaaaaaagat tggggacgag
    11101 atttcgccag aagcggtcca aaaagaattt gttttaagag attcttttgc taaaaaccaa
    11161 ttccagcagc tgatggctga tattccggca gaggagatcg aatgggtcaa acaagtagtc
    11221 gaactggctg aagataaact aaaagttgaa ttttcgccta atatttatct gacattgacc
    11281 gatcatcttc attatgcgat cacacgagca aaagaaaata tccaactgcc taatccatta
    11341 ttatttgaaa caaaaaaatt ttatccgaaa gaatatcaag cagcgaaaga ggctcttttg
    11401 ttcgtgaaac aaaagacggg tgtggaactt tctgaggatg aagccggttt tattgccttt
    11461 cattttgtga atagccaaca aggaaacgaa gacatgcaag tgacgatgac cgctaccaca
    11521 atggtaagag atatactgag tatcatcagt aaattcttcg gcatcgtgtt cgatgaagaa
    11581 tctttgaatt atcagcgtat cattactcat ctgcagtttt ttgcccagcg ttatctcaaa
    11641 ggggaaggat ctgacgagca agatgaattt ctctacgcat tagttcaagg aaagtatcca
    11701 aaagcgtttc attgtgtcga acggatcaat gagtatcttc gacaaacgca aaaacaggaa
    11761 atggggatcg cagagcagat ttatttaacg attcatattc aaagagtagt aagtgagaaa
    11821 aaaagcatcc aatcataagg attgttactg tatggcaggc aaaacctgga ttgaaaaaat
    11881 aggttcacga atgtttgtga ctgttttttt caatctgggt ttttattttt taaataaaaa
    11941 agaaagaagg aaaaaacaat ggataatcaa gcagttggtc gtcgtgtatg ggaagcggta
    12001 ggaggacaaa agaatgtcaa aagtttagta cattgtgcta cgcggttgcg attcagatta
    12061 aaagatgaat ctttagcaga tacgcaaaag ttaaaagaag acccggacgt gatccaagtc
    12121 gtacaaagtg gcggtcagta tcaagtagtg attggcagta atgtagcaga tgtttatcaa
    12181 tcgatcgtgg atgaggaagg tcttgcagtt tcggaggatg gaaaagaaga gtccaaaaat
    12241 attttgaatc gtctgatcga tattatctct agtatattta caccttttct aggggcgatg
    12301 gcagccgccg gggtattgaa aggattctta tcattggcaa cggtgatggg ctggctgaca
    12361 gctgatagcg gtacttatca aatattgttt gctgcagcag atggtgtctt taccttctta
    12421 cctgtcatgc ttgcttttac agcagctaaa aagttcaaag ccaaccagtt tttagcagta
    12481 gcaattgcta tggcacttgt gtatccagct atcacagcag ttgctggtgc gggagaagct
    12541 atcagtttct ttgggatccc agtgattctt tcaccatctg gttatacttc ttctgtcata
    12601 ccaatcattc tagccgtatg ggttcaaagt aaattagagc cattcgtaaa aaaagtgatt
    12661 ccgcaatttc tgcaaatgat tttagttccg cttgtcgttt tagtagtgat ggttccatta
    12721 acatttcttg ctttaggacc aatcgggacg gttgccggta atgctttagg cggtctcttc
    12781 aactcgattt atggatttag tccaatcgta gcaggtctaa tcatgggaag tttatggcaa
    12841 gtattcgtga tgttcggcat gcattgggga ttcgtaccga tcatgttttt gaatatcgaa
    12901 caatatgggt ttgatgtatt gatgccaatg cttcttccag ccatcttggc acaaggtggg
    12961 gctgctttag ctgtagcatt gcgaacaaaa gatacgaaac ttcgagcatt agggatttct
    13021 tctacagtga cttcgttatt tggaatcact gaaccaactg tgtatggtgt gacattacct
    13081 ttgaagaaac cattcattgc cgcttgtatt tctggaggta ttggtggagc tattatcgga
    13141 ttttccggag taaaagcttt ctccagcagt ttggtcagct tattgacgat tccgactttc
    13201 attaatacgg tggatggtgt agagtcaaat gtgacagtag cagtaatagc tacaggaatt
    13261 gcttttgttc ttgcattcgt aggtacattg attttaggat ttgacgaaca aacacaagac
    13321 aatcaattag aaaacaaaca tgcgaatgca ggagaaccga ttacttcagc acggcatacc
    13381 ttaaaaagtc ctttaacggg taaagtactg ccattgagtg aagtaccaga ccaagttttt
    13441 tcttctggcg tgatgggaaa aggtatcgct attgatccag aagtaggaga actagttgca
    13501 cctgcagacg gagagatcac gactattttt ccaactggac atgctgtagg gatcacaacg
    13561 actgacggag cggagatttt gattcatatc gggatggata cagtagaatt aaatgggaat
    13621 ggctttgaga tcttagtcaa acaaggggat ctcgtcaaag caggagattt attgattcgt
    13681 tttgacatcg aagcaattag agcagctggc tatagcgtaa tcactccagt tgtcattacg
    13741 aatactgatg catttgccga tatacttgaa ttagaccaaa aagagattat tgcaaacgaa
    13801 gatgttctgg caattgtaaa ataaggagga aatcaggatg caatcacgtt tttcagaaaa
    13861 atttttatgg ggtggagcaa ctgctgccaa atcaattaga aggggcttat gatcgagacg
    13921 gaaaaggact atccgttgca gatgctatgc ctggaggtaa gcagcgcttt gcgattatcg
    13981 gcagtgaaga atttgactgg acgattgatc aagaaaaata tatttacccg aatcatcgag
    14041 gaatcgatca ttacgatcgt ttcaaagaag atcttgcctt gtttgcggaa atgggattca
    14101 aatgttatcg tttttcgatt gcctggacac gaatttttcc aaatggagac gaaggtactc
    14161 ctaacgaagc gggattagaa ttttatgatc agctgattga tgagtgctta aaatatgata
    14221 ttgaacctgt gatcacgatt tctcattacg aaatgcctct tcatttggca aaagaatacg
    14281 gtggctggaa aaatcgaaaa ctgatcgact tctatgaacg atttgctcag acagtattag
    14341 agagatacag cagtaaagtc aaatattgga tgactttcaa tgaaatcaat tcggcctttc
    14401 actttccagc tttaagtcaa ggattagtaa aaagcaatgg agcaggagag taccaaaata
    14461 ttttccaagc atggcataat caattcgttg caagtagcaa agccgtaaaa atcggccacg
    14521 aattgcgttc ggatatccaa atcggctgca tgattatcta tgcaacaact tacagcatcg
    14581 atgccaatcc agtcaatcag gcagcaacaa tgatccaaaa ccaagaattc aattttttct
    14641 gcacggatgt ccaagtacgt ggagaatacc ctgcatacac agcgcgtacc cataaaaaat
    14701 atggagtcga tccggaaaaa ttagagcaaa cggaaaaaga cttccagttg ctaaaagaat
    14761 atccagtgga ctacatcgga tttagttatt atatgtctac ggcaatcaat gagactgatc
    14821 cacaagcagc aacttcagaa gggaacttgc tgggaggagt aaaaaatccg tttcttgagg
    14881 caagtgaatg gggctggcag atcgatccag aagggctgag aatcgcattg aatgaattat
    14941 acaatcgtta ccagaaacca ttattcattg ttgaaaacgg tcttggagcc attgatcata
    15001 tggaagagga tggcacgatt attgatgatt atcggatcga ttatttacgt cgtcatatcg
    15061 aagcgatggc taatgcagta gcagacggtg tagatttgat gggctatacg ccttggggat
    15121 gtatcgatct tgtcagtgca tcaacaggag aaatgagtaa acgttacggc tttatctatg
    15181 tggatctaga cgatgaagga aatgggacac tgaatcgatc aaagaaaaaa tcttttgatt
    15241 ggtataaaga agtaattgcc tcaaatggcg taaacttgta attaaagcag ctgaatgaaa
    15301 aagaacacac ttctcacatg tgatttgtgt tcttttttta ttttttatgt gctattcttt
    15361 tagtaatagt aaagcaaagg gtgacgttat gaaaaggaca cgtgtactat acttggaagt
    15421 cgcagataaa attaaagaag atattttctc aggaaaatat ccagtcggtt cgatgttgcc
    15481 gacagaaagt gaattagaag aactgtttca agtcagcaaa atcactgtcc gaaaggcaat
    15541 cgaactgctg gcatcagatg aatatgtcgc aaagaaaagt ggtcgtggga cgacggttct
    15601 aagcaatcgt ccttacaatc gattatccaa agcggcaagc tttacgcaaa tcttggagaa
    15661 ctcccgatat gaagtccgga aagaaacgct ggatttgcaa ttggtccata taaagaagga
    15721 agatcctttg ttttcctttt tcggtgagca agctgtttgt tttaagcgct tatattattt
    15781 gaacaatcag ccttatattt attttgttca ttatcttcca caagaaatga tgcaattcag
    15841 cagagaagaa tttggcaaag aatctttgta tcgtttgctg acgaaaaaag gatatactat
    15901 tgactcattt tctgatgatt tctcggcagt atttttaaca gaagaagaac aaggaattct
    15961 gaaaacagac gcacagatcg gcatcaaacg gatacggaaa tcgtctacgc aaattgggga
    16021 aatgattgaa tattcggagg cggtctacca tacggagctg catccttacc atattgatta
    16081 tgaaacataa ataaaaaaag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca
    16141 gccaaacaaa aatagcttct catatttttc gtttggttag gcttattacc gcagatacac
    16201 ggctgtttct ggattaccat ttattctgga taagggggtg tggcacgatt ttcagaagga
    16261 atctatgaac caatcactgt attgatttta gcttcgatgg taaccccatt cggcttgacg
    16321 atcgcttatt tcttagggaa aatcattcgt aaaaatatac tcaatcgtca agaaatcgat
    16381 acattgaaga ccgcttttcc tatggggatc tgtcagatca cagagggatg tttccctatc
    16441 gtattgaacg acttggcacg caatgtcata gcaacgggag tcggtgggac agtaggtggc
    16501 ggattaagca tgttttgggg cgcagatagc cgtattccgg catcgggaat gtttgcagtc
    16561 gctacgatga ccagaccatg ggcattcatt ggtgcattgt tagctggttc ttttgtcaca
    16621 ggaataacca ttttattatt gaaaaaacca gtagatccga atgcagaaat catccaagaa
    16681 gaaaaagagg aagaagacat atcttgggat gatttgacaa ttagttagga gaggcagaat
    16741 ggattacaag aaaaaagtac aatagatgta ccttgagaca ggaatggtat gaaagaatca
    16801 gagttagaaa gaatcgaata tacagatttt ggtttaggag cgatcgaaac tgtccagagc
    16861 atcggcaccc tccttgaaat gacgatgaag gaaaaatgag aacctttcgt tgtcgaaagg
    16921 gtctggtata ccttatgtcg aaggaaagga aacaaaaaaa ctttcctgta ctattccaaa
    16981 aaggaaagaa agttattata cagtaaaaaa agaaattatt cttcatgcta gagatcagta
    17041 cacgttcgag cccaatatca aacactagtt ccaggcaggc gcaacaggtg cggttatcac
    17101 tgaattttct tccagcagtg acgattcaag tgatattttt acaaatccag ctatttatcg
    17161 ttaacaaaaa gatttttagg agaagctgat gaacaaaaat gatcaaattt tgatcgttta
    17221 gttcgtcggc ttttttattt agagaaacat catttatcag tgaaacataa cacgagaaca
    17281 aaatgatttt ttcgttaata aaagaaacaa gtatttgttg tttaaaataa tacacgttat
    17341 tggcatatag gacgaaatcc cagtatcttc tataagtaag tcatttcata tcgatgaaaa
    17401 cacgttattt atttttgttt tatctataaa atggctgaaa aatgattttt ttctaaatac
    17461 tatatatagt ggcgtctatc ctatatacca ctaaaaaact acaaaatcta gtcttttatt
    17521 ttaaaaaaac acgtgtagaa tgaagataga cttttgagtt agggagtgtt tttttatgaa
    17581 acttactgtt ttttgcggtt cccgttttgg taataaagaa agttacaaat ttattgcgca
    17641 aacgttagga aaatacatgg cacaagaaga aatcgaactg gtttacggtg gttcatactc
    17701 cgggattatg ggagtgatta gtaaaacagt actggaaaat aacggaaaag tcacaggtat
    17761 ctatccgaat gggctttttg aaactgaatt gcctggaaaa gatgttacta catttattcc
    17821 tacagaaacg atcgatgaaa gaaaggtatt attatttgaa aagggagatg ctgtcctcgt
    17881 tttccctgga ggtctaggta cattggagga attctcacaa cttctttctt ggatagcaat
    17941 cggtctgacg cccgacaagc caatcggaat attgaatatt ggcggttatt acagcggctt
    18001 gcaacaatta ttagaaactt ttgcaaaaga ggattttatg gataaaaaat ggttggatca
    18061 agtaatcttt tccaacaatc cattggaact ggtcaatgaa ttacgagcag tatctattaa
    18121 aaatcagtta ttattgaagg aggcaaaata atgagtcaaa tatatttagc aggaccattt
    18181 ttttcagagg agcaaatcga tcgagtaagt cgtatcgaaa aagcgttgga ggaaaacaaa
    18241 acagtcacaa gtttctactc tccacgtcat caccaagaaa gcaattatga actattcagc
    18301 gctggatggg cgcaggaagt atacgaaaaa gatatggaag aactgacaaa tgcagaattt
    18361 gttgttgcta tccttgactt tgagcatcaa acaatcgatc caggaacggc ttacgaatta
    18421 ggtgtggcaa ctatgttgaa aaaaccgatg atcattgtac aagaagagac ggtacctacg
    18481 aacttgatga ttacacaaag tctccatacg tatttaaaat cagaccaagc tgtacgtgaa
    18541 tatgattttg aaacattgcc tgttgaaact tacgtaggcg agtacttata gaagaggaag
    18601 ggttgtatgt cagtgatttt gatttcagga acgattggtg caggtaagag cagtttgacg
    18661 gatatgcttg caaaagagat tgattctaaa ccattttatg aaaatgtcga agataacgaa
    18721 gtattgccgc tcttttattc aaatcctgag cagtacgctt ttttattgca aatcttcttt
    18781 ttgaacaagc gatttttagc aatgaaaaat gctttagtga acgatgacaa tgtgctagac
    18841 cgatccatct atgaagatag cctgttgttc catctaaacg ctgatttagg tcgggtaacg
    18901 gatatcgaag tacagcagta cgatagctta ttagatacga tgctaaacga gttagatgat
    18961 gttgctccaa aaaaacggcc tgatttgatg gtccatatca aagtttcttt ggacacgatg
    19021 ctagaacgta tcaaaaaacg tgggcgtgac tatgaacagc tagaaagcga tgagacgtta
    19081 tatacctatt atgaaacgtt gaacacccgc tataatcaat ggtatgaaga ttttgatgta
    19141 tgtcctaaaa tacaagtgga tggggataaa ttcgattttg tagaaaatcc agaagatgca
    19201 aaaaaggtga tccgtcagat ccaagaaaaa ttagctgaat tggaaggaaa gcctgctgga
    19261 aaatatttta cggataacca gcatgcaaaa ggtcaagaat taggctaaaa ataaaaaacc
    19321 aaacgacggg aaagaa
//
LOCUS       DAFEOM010000049        19192 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.050, whole genome shotgun sequence.
ACCESSION   DAFEOM010000049
VERSION     DAFEOM010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.050"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(<1..1416)
                     /gene="nhaC"
                     /locus_tag="LP524_001907"
     CDS             complement(<1..1416)
                     /gene="nhaC"
                     /locus_tag="LP524_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBL2234940.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(1819..3039)
                     /locus_tag="LP524_001908"
     CDS             complement(1819..3039)
                     /locus_tag="LP524_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBL2234941.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAPEKGVNALEALIQVFNGINALRLQLPKDV
                     NIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFEF
                     GLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTIQ
                     PTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQEH
                     QEIVARKKQEGATF"
     gene            complement(3070..3909)
                     /locus_tag="LP524_001909"
     CDS             complement(3070..3909)
                     /locus_tag="LP524_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBL2234942.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(3926..4606)
                     /locus_tag="LP524_001910"
     CDS             complement(3926..4606)
                     /locus_tag="LP524_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2234943.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(4608..5681)
                     /locus_tag="LP524_001911"
     CDS             complement(4608..5681)
                     /locus_tag="LP524_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL2234944.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(5737..5975)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(6018..6251)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(6328..6999)
                     /locus_tag="LP524_001912"
     CDS             complement(6328..6999)
                     /locus_tag="LP524_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL2234945.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(7086..9764)
                     /gene="mgtA"
                     /locus_tag="LP524_001913"
     CDS             complement(7086..9764)
                     /gene="mgtA"
                     /locus_tag="LP524_001913"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBL2234946.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            10423..10860
                     /gene="ndk"
                     /locus_tag="LP524_001914"
     CDS             10423..10860
                     /gene="ndk"
                     /locus_tag="LP524_001914"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBL2234947.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(10882..11748)
                     /locus_tag="LP524_001915"
     CDS             complement(10882..11748)
                     /locus_tag="LP524_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL2234948.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(11908..14001)
                     /locus_tag="LP524_001916"
     CDS             complement(11908..14001)
                     /locus_tag="LP524_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBL2234949.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(14750..15373)
                     /locus_tag="LP524_001917"
     CDS             complement(14750..15373)
                     /locus_tag="LP524_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234950.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(15532..16929)
                     /locus_tag="LP524_001918"
     CDS             complement(15532..16929)
                     /locus_tag="LP524_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL2234951.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(16961..17932)
                     /locus_tag="LP524_001919"
     CDS             complement(16961..17932)
                     /locus_tag="LP524_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL2234952.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            18306..18731
                     /locus_tag="LP524_001920"
     CDS             18306..18731
                     /locus_tag="LP524_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234953.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT         5550 a         4067 c         3231 g         6344 t
ORIGIN      
        1 ttgttctaat tgcgctgtct tgcttactgc ttcttgcggt ttcacaaatt tttttgagat
       61 tccccaacct gtgaaggcac tgaccatcga aaacacagga gaaagcaaac tgaaaaatac
      121 gaatggcaag taagtgagtg taggaatacc aaatgtactt gctacgaaac ttccggcaat
      181 tccccaaggc actaagtagt tgattactgt tccaccatct tctagcgttc tacctaatac
      241 agttggatca aatcctgctt tttcataagt ttctttaaat gcattaccag gcagaataac
      301 agataggaat tgttcaccaa taaaaatatt tactccgata cttgtcatca atgttgtaaa
      361 aatcaaattt ggtgtgttcg tcatctttct tgatactcgt tccatcacgg ttgagatcaa
      421 gccaaattcg atcaacaatc cacctaatga caaagttaga ataatcaatg acacagtagg
      481 catcatactt tcgattcctc cgcggctaag cagtgaatcg atttgttggt ttccagtatt
      541 tgaaacaaac cctgattcga tgaccccagc caacttcgtt aaggaagttt gtgggttttg
      601 aataaaaatc attcctactg ctacagcgat attcaacaaa atcgttaaaa ttgccggcac
      661 ctttttccat gcacacagta acattaaaaa taaaggaatc agtgaccaga ttgaaatagt
      721 aaagtttgct tctagaacat cagtcacttc acgaatagat gaaagacttg cggctttact
      781 tgtgtgtcct aaaatcatga ataaaaagaa agaaacaaca aatgccggga cagttgacca
      841 catcatatgc ttgatgtgtt tgaacaaatc ggcatcaaca atagctgctg ctagatttgt
      901 tgattcggat aatggagaca tcttatctcc aaaaacggca cctgaaacaa tagctccaac
      961 aaccagcgct ggatttaagc ctagtgttgt cccaataccg aaaaatgcaa tcccaatcgt
     1021 tgacataaca gtaaaggcac tccctacagc gctacctacg attgcacaca caacaaatac
     1081 agaagggaca aaccactgga cactgatcaa tttgaaccca atgaccatta gagttggaat
     1141 gatccctgct tgtatccata cagcaatcaa tgaaccgact aaaataaaaa taaagattgg
     1201 gatgattcct ggtttgatgc cattgatgat tccttcgtgc agccaatctg tcgggtatct
     1261 ttttatcact gcatacaaca tgaccattcc gatcgctgct aagatcgtga tattaggtga
     1321 gacctttaat ccgacaacgc caatcgcaat gcctaaaaca ataaccatta atacaagtgt
     1381 tgcttctaaa aaacttggtg ttcctttttt tcccatgata atctctcctt ttttagttgt
     1441 tcttatgttc taaaaaagtt aagatatcgg attactccgc cgcaattcat ccgcgattcc
     1501 tcctttataa aaataaaaag acctgtttta aactaaggac gaatgactcc gcggtaccac
     1561 cttaattaaa aacagatctg tttttcactt tcaaactttt attcaaatgt atggactttc
     1621 catgtaattc gtcaattagg cttgatcggt tcacagcgac caccgacttt ctgaacaaaa
     1681 cacctaaccg ctactgcgtg aaaagttgac ttgtacgaag tttaccttcc ataagatcat
     1741 ttgtcaatga aaaagaaaat attttttcat ttttctgaaa attattttta taactttcac
     1801 acacaagttt attttttact agaaagtagc gccctcttgt ttttttcttg ctactatttc
     1861 ttgatgttct tgcttgattc tttctaatag ctttggctct ttcaacagat caagagctgt
     1921 attggcgagt agttctgctg caatcgcaat agaagcaagg cctttgtcac ttttagcagc
     1981 agctttgaat tcttctgtgt gtccggcaat atatgtctca gagattgcta ccgtaggctg
     2041 gattgttggt atgacttggc tgacatttcc tacatcagaa gaaccgagac ttgttctttg
     2101 ttccgtttct atttcttcat ctggaacacc tgcttctttt gcatgctgga agaataagtc
     2161 atcaaaagaa ggagtaacga tgacatcatc aactgcattt tggaacaatc caaactcaaa
     2221 agtcgtgccc gtacttatcg cagcacctcg tacgatattt tccacttttt catatacttt
     2281 atctaatgtt tgccgatttc ctgcacgtaa atagaaacgt ccagcagcgt aatctggaat
     2341 gacgttggca gctactcctc cgtctgtaat gatcccatgg atattgacat cttttggcaa
     2401 ttgcaagcgt agagcgttga ttccattaaa cacttggatc aatgcttcca aagcatttac
     2461 tcctttttca ggtgctgctg catgggaagc gacaccgtaa aatttgatgt cgactggatc
     2521 attcgctaaa gattctgtcg ttttcccata acgaaaagct ggatggacgc ataacgctac
     2581 atcgacatcc tcaaaaaaac cttctcgaac aaagctgcct ttagctgatc cgttctcgcc
     2641 cccttcttcc cctggggtgc catatacacg gatttctcca ccagtttcat caatgaattg
     2701 cttcactatg ctggcggcta atacggagta agtaccaaac aaattatgcc cgcatgcatg
     2761 accaatacct ggtaaagcat cgaattctgc caaaaaggca attgtgggac cgggtttatt
     2821 ggaaatataa cgtgcatcaa aggctgttcg gtgtccagct acttcttttt ctacccaaaa
     2881 cctttctttt tctaattgtt taatcaacgc tgctgaggaa taaaactcat agttacttac
     2941 ttcaggatgt tcgtggatat ctagagcaag tgcttgatat tctggtaaac gatattggat
     3001 tagatccttc aattggtttg ttgagattgt tgttgtcata aaaaattcct cctatgctag
     3061 tcatttttct tattttctcc cgaatgtttc ccatgcaggc acgcttgatc cttttgatgt
     3121 tttctcgatt actttgactg tgtcttctgt ttgataagcg tctactattt tttgatacgt
     3181 tttattgttt tcatcttctt ttcttgcaac gatgatattc acataaggac gtgcatggtc
     3241 atccactggt tctaagaaga tggcatcaga agcaggatta aatccagcat ctacagctac
     3301 accgctattg atcactgaaa tgtctacatc tgacaaagca cgtgctgttt gggaagcatc
     3361 taattcagaa atgtctagat tttttctatt ctcggtaata tcagaaacag tgggcgcttg
     3421 tccttttgcc ttatccactt tgatcaatcc agcagtttgc aataacagaa gagctctgcc
     3481 gccatttgta acatcattag ggattgctac ttttgctccg tctttgatat cagctacatc
     3541 cttcaaagtg ttagaataaa ttcccagcgg tgcattgaca gtatttccga tcgatactaa
     3601 atcagttccg tgttctttat tgtaattgtc taaaaagaat tgatgttgga aagaattcaa
     3661 atcaatcgat ccatctgcta aagccacatt tggttgattg taatctgtaa atttcacgta
     3721 ttccaaatta atgttctcct ttttcaaacg gtctttcacg ttgtcccata catctgtatc
     3781 ttctccaata atccctagtt ttacgatagt ttcttttttg tcagttgtat ctgcttttcc
     3841 ttggctacac cctgccaacc aaataattgc tacaaatgta atgactacaa aacgaataat
     3901 ttttttcatt ttttctcctc cactttcaat gtgtaatttt tttaatcagt tgattactga
     3961 taaattgact taaaaaaact aagattagaa tcaataatgt agccacaatc gttacatctg
     4021 tttggaatcg attataccct ctcgatatag caagattccc taatccgcct gcacctactg
     4081 ctccagccat ggtagtcagt ccgatcacgt tgatgatagt caacgccgag acccgaatga
     4141 taccaggcaa accttctaac aaatacacac gtaaaatgat accgattgga ctggttccca
     4201 tcgcttctgc cgcttcgatc accccaggat caacttctag cagtgcgttc tcgatttgac
     4261 gagcaaaaaa cggaacaact ccaaaaatca atggcactaa agctgctttc gcaccaatcg
     4321 tcgttccaac taagaaacga gtagtcaatg ctaaaagaga aagcaagata atgaaaggga
     4381 tggaacgaat gatattgatc agcttatcaa gtatttcaaa tagcagacgg ttagctagta
     4441 atccattagg gcgtgtcact actaacgtca cacctaagat gattccaatc atcccgccaa
     4501 tgaatgctgt ccaaaaagac atatatagtg tttgcttcgt cgcttcgaca aattcatccg
     4561 gaatcaaata aacattgggc aaatatgttt taaagaaatc agtcatttta tcttcctcct
     4621 tcgatcactt tgatacgact atctaaatcg tttttttgta aaatatttat ttttacacca
     4681 gatttgatca aataatccaa tgcttttttc cgctgtttgg tttctccagc taaagtaacg
     4741 atcaaactcc ctaacggcgt ttcttgtaat aactctacat tgccatataa aatattcgct
     4801 gttacttgat atttgctgta cagatgagcg attagaggtt cgttcgtctg atttccaata
     4861 taacttaatt ccaccagcca tttattggcg atctgatcgg caaaagcaga atgggcaatg
     4921 atcgtttcta atgcttggtc aagatgagta gctgttcgaa taaaatcttt ggtcaattct
     4981 tcttcaggat tgctgaagat ctggatgctg ctaccttttt caacgattct tccatcttcc
     5041 ataaccgcta ccttgttgca gatttctttg acaacttgca tctcatgagt gatcaggacg
     5101 attgttaaac ctaactggcg attcaatttt tttaacaatg caaggatctg aagggtcgtt
     5161 ttcggatcaa gtgcactggt tgcttcatca cataataata ccttaggttc attcgccaaa
     5221 gctcgtgcga ttgctactcg ttgtttttgt ccccccgaca gttgtttagg ataagcagat
     5281 gctttttctt ccaaaccgac aagttccagt agttcattca ctttttgacg acgttcctgt
     5341 ttcgattttc cagaatactt taaagaaaaa tcaacgttgt caaaaatagt tcggctttcc
     5401 attagattga aatgctgaaa gatcataccg atggcctttc tttcttttcg caattctttg
     5461 ggagagagct gactcagatt cgtctgattg acaatgactt ctcctgctgt tggtttctgc
     5521 agcaaattca tcacacgaac taacgtactt ttccctgcac ctgagtaacc gacgattcca
     5581 taaacatcgc ctttttcaat cgttaaatct acagaatcta ctgcctgtat ctctttttct
     5641 ttttgtttga aacatacaga aacatctcgc aattcaatca ttaaaattcc tccttatcta
     5701 aaaaaagcgt cctcaagaaa atgaaaatgt cattttcttg aggacgcttt tttgcgtgtt
     5761 accacctcat gttcaagctg tgttcacaca caactcctct tcaagtacga tcatactcta
     5821 gcaagatatc ggtcgcagcc gtcatcgcct tacatctatg ctcgggatga attgctcgat
     5881 ggccattttc caatagtcgt tccttgcttc ttctcagcta ccgaagctct ctgtcaaagg
     5941 ttcttctatc gtacttacca tttcaatgca aataatattc aaaaaaaaag tccttttact
     6001 aacataaact atgtcagtaa aaggacgacg tatcgcggta ccaccttttt tcggaagttt
     6061 ccttccctca agcccaacat ctcaaaaaga gagattggcg ttcgttttaa cggtcgaaca
     6121 gttccgaatt tgcctactta tcgacaaatt attctccaag gccattttca gcttttctta
     6181 cttgactctt ctcaccagcc agagcctctc tgcaaagttt ccaaacttac tttccttttc
     6241 aacgaaatcg atattttgtt gttgggatta atctacatga ggaaaactga aaagtcaagt
     6301 acgaaattca aattattttt tctatattta tttttctgtt tttttattta acatttcaat
     6361 aacagttcga agatcttttc cttccgcaag aagtttcaat ttttcctttt cttcgacggt
     6421 ataatttcgg acaaaactgg aatcaggtat caaaacaacg cctaatcctg ctcgtgaagc
     6481 agctgttgct cctactaaag aatcttccac tgcaaaggct gaactagctt ctaatcctgt
     6541 tcgttccaaa gctgtcagat aaggaatcgg tgaaggcttg ttctccgaca cttcgtctcc
     6601 gaatgtaaac gtgtccaagt aatcagctaa tttaaagtga tccaagatat ctgttgctct
     6661 tttcgtaaca gtggtcgtag caatgcctag tttcatatga cgttctttta agtctgccaa
     6721 tatttctttt gcatatggtt ttaagtggat ggctccgctt gtcagctgtt gataaaaata
     6781 ttcttcacgc ttcgctcgga cttcctgtac tttttcaggg ctacctgctg ctttcactaa
     6841 aatatcaaaa ctttgtttcc agttttttcc gccccaagcg tcaacgattt cttttgggat
     6901 atggatatca aattgctgaa atccccaaag ccagccatca cgatacgttt tttctgtgtc
     6961 aactaacaaa ccatctaaat caaatattgc acctttcatc tatacattaa tcctttccaa
     7021 aacaatacac atagtttagc acgactggtc caaaaagaac agaagcaaag aaccagcctt
     7081 ctcgtttata agagttcttg gaatcgacgg atatagattt tcttcacagc tgtcacaagc
     7141 gccagataag cgatgactgt agcaaataac cataaccagt aattgccagg aagtggcatc
     7201 aagcctaatt cagcaccaaa acttgtaaaa ggaaggacag aaccgacaat aattcctaat
     7261 gtagtgataa caaacatggc aaatgttgcg ttgctttgga taaatggtac tttcggtgta
     7321 cgcagtgcat ggagcactaa ggtttgtgtc catagtgatt cgacaaacca tcctgcatgg
     7381 aataatgcga tgaaaatcac tcgctgtgaa ctatccaatg tatggaaact tccgcccact
     7441 acagcaggac aaataacaaa atacatcaac aagtaagtag taatatcaaa tatcgaactt
     7501 gttggaccaa agtaaaccat gaattcccca atactagatg cattccattt ttttggctcg
     7561 tccaaatatt ctttatccat ccgatcccat ggcaaggaaa tgcaggaaac atcatagatc
     7621 agattcaaaa acaaaagttg taaaggcagc attggtaaaa atggcaggaa cgtacttgca
     7681 atcagcacgg aaaacatatt gccaaaatta gaacttgctg tagccttgat atatttcatg
     7741 atattcccaa aagtttctcg tccagaaaga aggcctcttt ccaaaatcgt caaatctttt
     7801 tctaaaagga tcacgtctgc agattctttc gcaatatcca cagctgtatc tacagaaata
     7861 ccgatatcag ccactttcat tgcaggagcg tcattgatac catctcctaa aaatccgaca
     7921 gtatgaccat ttttacgtaa agctgtcgtc aaacgtgctt tttgctgagg attcaatttt
     7981 acgaatactt catggtcttc agctacttgt gctaattcat gatcactcat ttccgagatt
     8041 ttctcaccgg taaccaattc attgatctct agacctactt gacggcacac tgaacgtgta
     8101 actaattcgt tgtccccagt cagaactttt acagctacac catgttcttt cagagctttc
     8161 aacgcttgtt tcgttgtttc ttttggtgga tcaaggaaag caagataacc aatcaaaacc
     8221 atgtctgact catctttgac tgaaaattct ccaacgacgc ttggattggt cttttgtgcg
     8281 acaccaatca ctcgtaagcc atcttcatta agtgactgta cttttgcaag tacggcttct
     8341 tttcgttctt ttgttaatgg tgtaacagta ccgccaagat caataaattt agaaatgttc
     8401 agcatttctt caatcgcgcc ttttgtgatc atttgtgttt taccagctgg atcagcaacc
     8461 acaacactca tacgacgacg ttcaaaatca aaaggaattt catccacttt ttgataattg
     8521 atcttatctg tatctaaagt ttctgttgct tcatcaatga tagccacatc taacaaattt
     8581 ttcagccctg tttgatagta gctatttaga tacgcatggc gcaaaacacg atcatcttca
     8641 tttccttcac aatccatatg atattcaaga atgattttat cttgtgtcaa tgtccccgtt
     8701 ttatccgtac ataacacatc gattgcacca aagttctgga tggcattcaa atttttgatc
     8761 actgttcctt ttttggccat cacacttgct cctttgacca aattcgtcgt aacgatcatt
     8821 ggaagcattt caggtgttag accaactgct acagatagtc caaaaagaaa agcttctagc
     8881 caatcccctt ttgtaagccc attgatgacg ataactgctg gagccatcaa tgacataaat
     8941 ttgatcagta gcatggatgt tttatggatg cctaattcaa agcttgattg aattggtttt
     9001 tcagatagcg tttctgctac atgaccaaat agtgtttcat ttccaacaga aacaatgatt
     9061 ccttccgcac ttccactgat gacattactt cccataaaga caagattttc gtaacttgtt
     9121 tcgctttgct gatcgtcttc acatactttc gcatgctttt ctactggata gctttcccct
     9181 gtcaatgctg cttgggaaat gaataaatcc tttgcggaca ataaacgcat atccgctggt
     9241 atcatatccc ctgctgaaag ccgaacaaga tctccacaga cgatttcttc cataggaatc
     9301 tctgtgtact ttccattacg ttttacagct gccgttactt tcacaagtga tttcagtttt
     9361 tcagccgctt gattggaacg tacggactga atcaacgtca ttgtcccgct gacgataacc
     9421 atagtgaaga taatggctac accgaaaaga tctttatctc caggagctgc taatacatat
     9481 tccgtgatga atgaaataat tcctaatgcg atcaacacta atgtaaatgg cgtgatgtat
     9541 gctttcaata tttctacaat gaatggtgtt tttttgccat atgaaatcgc attttcaccg
     9601 taattttctc ttgaacgttc gatttgttct tctgtcaatc catttgctga tgcatggaat
     9661 agttcaaata attcttgttt cgtttttcca gcaaaagctg catagatttt ttgtgtatct
     9721 ttcttttttt ccatctcaat ctctcctttg tgtccaaaag acagctgttc tcgctgcttt
     9781 tggtctttag ctattcatcg gatcgataga gaaaaaactg aaaggggctg agaacagata
     9841 gataatagcc ctggcctttc ctctagatcc ttgtttttcg aatgactgtc atccatttgt
     9901 tctcatctcc tttactaagt aattttaatt catgacttcc aatcattttt cgaatagtta
     9961 tttttccaat tagaccacct cctttttatt tttcaccgat ggggatttgc ggcaacaaaa
    10021 aaacctctcg ccaaatactc agcgaaaggt ttgaaaaagc atattaaaaa aagcttctca
    10081 gatctcatcg ttgagttttg acactgtaca acgtaaaaag cagatacctg cttttagcta
    10141 ccttatgaaa agccttatct cgacaattcc tgttctaccc attggcgtct ctcgacgttt
    10201 ttgggcagta gcctatgttt gtataggagc ctcacctaac aaatcttatt cggttttcct
    10261 tcgttaatat aacaactaaa aaagaggatg tcaaatcaat tttccatgtt tttcatactt
    10321 tttttaaaat acacacgtta tattcctgtc tcacgcggtt ttatttgtct cattttccac
    10381 ctcccgctat aatagaaata tagtaggaaa cgaggtgctg ttatggaaag aacacttgtt
    10441 attatcaagc cagacggcgt gagacgccat ttagtcggaa gcatcatcca acgttttgaa
    10501 gcaaagggac tggccattgc tgaaatgaag tttgaaacta tgactcctga attagcaaaa
    10561 gaacattatc agcacttgac tgagcgcagt ttttttgatg aactgatcga ttacatgact
    10621 tccggtcctg tcgtttatct tgtgctggtc ggcgaggaag tgatcgatat cgtccgtaag
    10681 atggttggag caacaaaagc tgccgatgct gttccaggta cgatccgagg agactacgcg
    10741 ctgccgggga ctgaaaacat cattcacgct tctgattccc gtgatgctgc ggtaaaagag
    10801 atcgcccgct ttttcccgcc attcgatata aacaaaccgt ctgaaaaagc ggcgaagtga
    10861 tacaaattct aaactgctgg tctagttgct gggttcagca atggccagca gttttttctc
    10921 taaggacctc cgttgattct ttcctaacat atattcgagc actgtgaaga attcttggta
    10981 gtctttcgtc ttttcttccc ctaaagcagc agaaatcaat ctttgataat aatctgccat
    11041 caatgtttca tagatcactg tacttctttc tttatgtgct aagctccgtt tcatatcttc
    11101 attcacacta cgtgcctttt tcaaccgacc attttctata taaaacgaag tcaggttcat
    11161 taaaaaagta aagagatact gctcatgatt gttcaactgt gctaatcgtc gcattctttt
    11221 tttgattttc aaataattgt tctcaatgaa ccgatcactg aatatccaag gaaaatcagc
    11281 tagaatagat agttcaaatt gaccatagtc tggtatctgc atcaagtatt cttcaatgat
    11341 tcggcattca taacgtaaaa attcaaattc tgtctcagat acttctgttt tttcgaccat
    11401 gtgaagattc aaaaccaaat aatacagatc ttctgttttc ttaaaatgcc tacggttatt
    11461 gctgatctct ttataaggat caaagctgtc ttcttgtacc cgatctagca ttcgtctttt
    11521 tttctttaac tttattggtt catccagctg gtttcgcatc cattcatatt ctgcaagtgt
    11581 catgttcatt cgctctaaaa atgatctcaa tgttacaaat gacatttttt tcactgaatt
    11641 tgtctcaatc ccgactagag aagtcttaaa acagattcct tccgctaatt gggactgtgt
    11701 caacttcttg ttgactcgaa gtttcttgat caattcacca tcatacataa tagttctcct
    11761 ttttaattta aatttttcca cattcaataa atagatacgt taaaaaggaa aaatacacga
    11821 aaaaaataaa aaaatgtttt tttcataaaa aataccaacc ttcaagcatt agaatgttgt
    11881 ctaactactg aaggttggtt tgattgttta ttgattattt ttttgctgtt caacgtctgt
    11941 tcttgttact ggatataagt tggttgttgt tttattacca ttttttgaaa agacacttgt
    12001 cgaatgttcg cctttttgct tttcgattgc ttcattacgt tccgcttcat ttttatagtc
    12061 atattttttc ggatcaatcg tctcaaatcc gtttggcaca taaaaccgca agagattttc
    12121 ctgattcagc ttatctgaaa gacttaacgc agtcttgact ttttcctgat ctttggagat
    12181 ttcctcatcg attcctgctg ctttcgtatc gatgatttct cctgtctgat tattgtaagc
    12241 ttttcctcct aggacggtgt atttgggtgt cacaaagttc ccatttcgaa aagcaactac
    12301 ttgatcatgc tgagaagata ataagtctgt accaaaatga agataatctt tatcatcgat
    12361 tcccaataga tgtagtaatg tcggcaaaac atcgatttct ccaccgtatt catgattgac
    12421 tgtcccttta gtataaccag gcatatggat catcaatggc actcgttgca tctgtgcgtt
    12481 gtcaaattca tcccatgtat ctggatcttt accaagagcg cttgctaaat ctttattatc
    12541 tgtattcgag atcccaaaat gatccccgta aatcacaaag attgaatttt ggtaaatacc
    12601 agattttttc aaatacgtaa aaaattgttc caatgattga tccagataat gtgccgttcg
    12661 aacataatta tctactgtgc tattgccagt attcaaagat ggaaagtcga aattcttgtc
    12721 atccgtataa taaggcgtgt ggttggttac ggaaagaaat ttcgtgtaaa acggctgttg
    12781 tagatgctca agatattggg cagattctct gaataaatct ttatctaata tcccataacc
    12841 taacgtttcg tcggattcat caaaataaga tcgatcaaag aaattgtcat aacctaagtt
    12901 tttatataca tgatctctat tccaaaaact agctacattc ccatggaaaa cagcgcttgt
    12961 atagccttgc ttttgtccaa ggatcgctgg tgcggcttga aagacattgt ctgacccaag
    13021 ttctgtgaat aatgagcctt gaggtaaacc aaatgtgcca gtttccaaca tattttcagc
    13081 atcacttgtc tttccctgtc ctacttcatg aaagaaattg tcaaagctga tcgttgcttg
    13141 attctggaaa atactattta aaaacggtgt gacttcttgt ccgtctacct tcatattaat
    13201 caaaaactgc tggaaacttt ctaaatggag aacgatcaca tttttccctt tagcgatccc
    13261 aaatgtttcg ggatttggct ctgcataatg tttctttgta taatctaaaa cttcatcaat
    13321 cccattactg cttgcatgcg cgcgaacact gctagtcatt cctgttttga ttccgtcata
    13381 aatagtatac gcatcgattc ctagatattt gacaatatat gaacggtcaa atgttcgctg
    13441 cagcaattgt ggacggttgg cttcgctcag cgctagattt cctgaaaaaa taagccctga
    13501 aagacaggta acagcgatag ccatcggttt gccgacaggg ttgcttttga taggtatttt
    13561 cttgaccttc aaccaaacca gtagcccaat gaacacagcg atatccagcc agtagatgac
    13621 atcatggggc ttcatcaaag aaaaggagct tcctgacaat ccttgcgaca ctttcgaaaa
    13681 gcctaataca gatttgactg taatgaaatc tgtgaattcc cgataaaaaa taatattcaa
    13741 gtacaacaaa agcgtattta aaatatcaat gatcaaaaga atagctaacg ctggctttgg
    13801 ctttttaata tacaaagaaa ggccaaaaaa caataaggta gtcgcaattg ggttgatcca
    13861 caagataaag tactggatcg taccttccac acctaatgaa aaatctgcat agtaggcaat
    13921 gatcgtcttc agccagaaaa agaaaacagc tgtcaaaacg attgctaaac gattttggca
    13981 caatttttta agttgtgtca cgtatatcac ttctctctat tcacattttt tcttattttg
    14041 ctcctgaaaa atgaaaattt cctaaaaaag ttgttatctt aagatttttt tattctttct
    14101 aaagattatt ccgtccactt ttcacagcat aaaaccgctg cgatgtaagc gattaaacta
    14161 atgaagagat aaaagtcttg tggtgaccgc ttcactccac ggtattcaaa agccagtttc
    14221 tcggtattcg tacaaaaaaa gaggctgtga caaaattctt gtcgcagctc cttatttctt
    14281 gatgcaggac agctttttca caacttcttc cttaaacggt gttctcctgc caactcctcg
    14341 gccacaagtc acaatattta taaaacatga gaagctgttt tttgaaattg ttccttgtgg
    14401 ctcagagtta aacggcaggc tcaagacctc ttgatacacg gtgttcaatc agctgaccct
    14461 cggtattaga ttatgtcagg acgctaattt tacaacctct tggtaaacgg tgttcaaaag
    14521 cttgcatctg cggtaataag cccaaccaaa cgaaaaatat aaggagctat tttttgaaat
    14581 tgctccttgt ggctcagagt taaacggcag gctcaagacc tcttgataca cggtgttcaa
    14641 tcagctgacc ttcggtatta gctcaaaaaa cggcaaaata tgaggaacta ttttgccgtt
    14701 tgtttctcta atactcaggt caaaacgctg atttcacaac ctctgttttt cattgtccat
    14761 aggattgata ttgtccttgt gatgactcat tcgtaataat tgaggcttga ttttcttcac
    14821 ttcccgttat cgtttgtcca ctgccagaat ctgaaggact tgttcctgtc gactcgtttt
    14881 tttgagaaga gacagttgtt ttcttgactt gttcatttgt ctttttccat tccgctaaac
    14941 gatcggcgac cacttttttg tctacttttt cagaataagc ggtgatcact tttccatttt
    15001 tcaaacgggc tactttgtcg ccatcatgag tcaaaagcaa aacggtcaaa tgcttgtctt
    15061 taaattgata ttgtctgaga gaaatcgcac taagtacgtc atcgatatta ataaattgat
    15121 tttcttcttt tccttttggc aaataaatgt ccaattcgcc tttttcgtaa ttggcagtta
    15181 ttttatactc cgcccaccaa taagttgaga attctttttt gaaattatct actgattctt
    15241 tgcttgctac gattttttct gtttctttct gagcaagatc tgctgtcatt tttcctgaaa
    15301 atgctgcgga taaaataagc agtaagaaaa aaccgaataa ccaaaagaga agaaacggtc
    15361 tttttttcct catgctttcc tccactccta tttaattttg actattctat tatatacaaa
    15421 aaatgaaata gaaagacaaa aaagttgaaa aagatcgtaa ataattgaac ttctttttca
    15481 caaaatggta agaatggaga atttcacgca tttgctctca actattcttt cttaagcctc
    15541 aaatccgttt ttatctgata cgtctttgaa ccaatgaccc gattttttga ttgttctttt
    15601 tccatcttgt gctaagtcta ctgaaataaa gccatatcga ttcttatatg cattggtcca
    15661 cgaccagtta tccatacatg tccacatatg atagcctaaa caattggcac cttcttgaat
    15721 ggcttgatgg acatatttca gatgagattg gacaaattcg atccgataat catcgtggat
    15781 catgccatct tctttgataa atcgtgcttc gttttctaca cccataccgt tttcagaaat
    15841 aaagcagtcg atatttccat aatgatctcg tacattgatc aaaatatcat agattccttg
    15901 ttcatagatc tcccatccgc gataaggatt cattttacga ccaggcattt cataattgtc
    15961 gaaataatcc tcaggcattg gaccgttttt agtatcgatt ggtgtttctt ttgcttttac
    16021 ccggcgtggc tggtaataat tgatacctaa aagatctact gtattttctt tgataaccgt
    16081 caaatcctct gcatggatca caggcatgag atcaagttct tttactatct caatcaattc
    16141 acttggaaac tcgcctttta cagctggatc aaggaatgaa cgattgaaga acgcatctgc
    16201 tatttgcgct gctttaacat cttcagggtt tgattcgtct cttggatagc ttggtgtcag
    16261 attcaagatg ataccaataa caccaccttg ctccatttca tggtaagatt tgatggctaa
    16321 cgctgaagcc aacgcttcat gatagccaac ttggatcgct tttttcatat ctgactcttc
    16381 tggataatgc catcgatata aataacctgc ttcgactgga acgattggtt cattatgagt
    16441 aaaccatttt tttactttcg aaccaaacaa ttcaaaacaa cttttggcat atttttcata
    16501 ggcttcaacc gtttctcggt tcaaccatcc gcctttttct tgcagataca tcggcatatc
    16561 gaaatgatac agattcacaa agggttcgat accgttttca atcaactcat caaaataacg
    16621 atgatagaaa cgaacggctt cttcgttgat tgttccagat tcatcaggta tcaaacggct
    16681 ccattggata gatgttcgaa atgaattatg cccagtctgt ttcatcaact gtacatcctc
    16741 ttgatacttc gtataaactt gcgaggtttt ttcaggtcct acttgatcaa agaacttttg
    16801 cggttctttt ttgtaccaat aatcaaaaat gttctctcct tttccatcgc ctgaaatcct
    16861 tccttctgtt tgtgggccac ttgctgcaga accccaccaa aaatttgctg gaaattgata
    16921 ttttttcatc aaagcacctc tttttctttt ttagttcgtt ttagttgatt ccatgtatga
    16981 tattttccat cacaatcact ttttcatttt cttcctcttc ttttttgatt cgttttaaca
    17041 gctgttctgc tgctcgttcc ccgattttat ctgttggtgg cgtgatcgtg attttcggaa
    17101 ggatactcag atcgttttta tctaaataat catagtgact gaacgcaata tccttcccga
    17161 ttctgacacc ttcttgattg aaacattcga tggcaccaat cgacatattg taattgctga
    17221 caaaaatagc agttgcccct ttttctaata atgcttttgc cccgtaatag ccgctttttc
    17281 tagaatagtc tcctgtaaag acctctaatt ggatcgatgg atcattttgg accgcttctt
    17341 tggctcctgc taaacgttct cttgctacgt agtctgtttg agtggcagca ataaatccga
    17401 tttttttatg tccttgttca ccatgatgag cgatcacttt tgctacactt tctcgatcat
    17461 tcactaatat tgtatcaata cctgctggtc cttctggaca attcaatgcg ataactggaa
    17521 tactcatttt tgtgagcatt tcaaatccag accactcttc ttcggataag aagacaatca
    17581 gcccatcgat cacatgcttc atcaggtatt caattttttg ttcaattttt tctggctgat
    17641 cggcaaaccc gcttaataat aagctatacc cattttcttc agctactctt tcgatccctg
    17701 agacaacttc cgacccaaaa cggctggaaa tcccgtttat caaaagtcca atagagaagc
    17761 ttcgattatt cttcaaccct ttagcaatga tgttttcttt atagtccaac gctgaaatcg
    17821 cttctgctat ttttttcatg ttcttttcct tcagttgctg accattcaag taacgggaaa
    17881 ctgttcccac tgaaacacca gccatcttag ccacatcacg gatcgatgcc atacattctc
    17941 actcctgcca gttacatttg atttctcttt cttgttgttc gattatgatg accgcattct
    18001 ttgcatagtg ttgcttgtca tcaatatcca tataactaaa gtcttcagta gagcaacttc
    18061 ctctcactat taattatact taaacaagta tttgaaccgg ttcattttta atatagcatt
    18121 tttttaaaag attgcaacac ttttttaaaa cgctttctga aagtggcgga ttttttgcct
    18181 ttttaaaatt ttattctaaa atttactcta tttgtgggtg ttttgtgcgt ttttctcttc
    18241 catccgtttg aagttttaca ttccaatggt ataatttctt tatcctatca aatgggggaa
    18301 gtattttgaa gaaattaatc gtactattta ttttgtctgc gggacttttg acattgactg
    18361 gctgtgattt gtttggcggc tctgaccaat cctcggacga aacaaccgat tcttctgtct
    18421 taaaagcgga aaaagcagat accaccaaca gtgtatatac gctaaaagta cgtaaagata
    18481 tggatttatc cgcgggacag ttgacattta cagataaaaa aatggaatgg acacgcacgt
    18541 atcagacaca aggaactgat tcttctgaat catctgaaga aaaaacagtg ctgacagata
    18601 tcaagatcac taccaaagat gacgaatata ttatttcagg aaaagaaaat aaaaaagaaa
    18661 caacagtcat atttacaaaa atcggtaatt accgtttgaa ggatgaggat ggcaacatct
    18721 acagtctata gtgccgtctg ttggatcttc ttttgtttct ccccttcttt tctgctatag
    18781 taaagaagca agatgattgt aaaggagacg aaatggatga tcgatagtcc tgattattgt
    18841 acatgtaata acaacaaaaa tatccataca gaatttaatg accaaggaag cttgagcata
    18901 tgcgacaact gtcacaaact agtcaaagat tcatttaaaa catttgaatc ttatcctgtt
    18961 caaaattatg taaaaagtag aagaagataa acaaatccaa gcaaaactca ctatggctga
    19021 tttttatgaa cttcaaactc agcttcttcc tttgatcaag cttcatcagt aaaacaaaca
    19081 gtggatcatt tcagtttaca aatcaaagag taaagattta tttatttcaa atatttgaat
    19141 ataaaataac caaacgatag aaaggcttat tctacatcac gtagtaattt tt
//
LOCUS       DAFEOM010000050        19180 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.051, whole genome shotgun sequence.
ACCESSION   DAFEOM010000050
VERSION     DAFEOM010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.051"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(32..1183)
                     /locus_tag="LP524_001921"
     CDS             complement(32..1183)
                     /locus_tag="LP524_001921"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBL2234954.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1196..1633)
                     /locus_tag="LP524_001922"
     CDS             complement(1196..1633)
                     /locus_tag="LP524_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL2234955.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1648..3414)
                     /locus_tag="LP524_001923"
     CDS             complement(1648..3414)
                     /locus_tag="LP524_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBL2234956.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3678..4355
                     /locus_tag="LP524_001924"
     CDS             3678..4355
                     /locus_tag="LP524_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2234957.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4358..5485
                     /locus_tag="LP524_001925"
     CDS             4358..5485
                     /locus_tag="LP524_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2234958.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5685..6125)
                     /locus_tag="LP524_001926"
     CDS             complement(5685..6125)
                     /locus_tag="LP524_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL2234959.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6136..8169)
                     /locus_tag="LP524_001927"
     CDS             complement(6136..8169)
                     /locus_tag="LP524_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBL2234960.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGLFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8171..9874)
                     /locus_tag="LP524_001928"
     CDS             complement(8171..9874)
                     /locus_tag="LP524_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL2234961.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9968..10918)
                     /gene="manA"
                     /locus_tag="LP524_001929"
     CDS             complement(9968..10918)
                     /gene="manA"
                     /locus_tag="LP524_001929"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBL2234962.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10957..11982)
                     /locus_tag="LP524_001930"
     CDS             complement(10957..11982)
                     /locus_tag="LP524_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBL2234963.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12191..13060)
                     /locus_tag="LP524_001931"
     CDS             complement(12191..13060)
                     /locus_tag="LP524_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HBL2234964.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13519..15411)
                     /locus_tag="LP524_001932"
     CDS             complement(13519..15411)
                     /locus_tag="LP524_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2234965.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15408..17135)
                     /locus_tag="LP524_001933"
     CDS             complement(15408..17135)
                     /locus_tag="LP524_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2234966.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17151..17801)
                     /locus_tag="LP524_001934"
     CDS             complement(17151..17801)
                     /locus_tag="LP524_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBL2234967.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18005..18301
                     /locus_tag="LP524_001935"
     CDS             18005..18301
                     /locus_tag="LP524_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBL2234968.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18493..19113
                     /locus_tag="LP524_001936"
     CDS             18493..19113
                     /locus_tag="LP524_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBL2234969.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
BASE COUNT         5451 a         3954 c         3381 g         6394 t
ORIGIN      
        1 ttcgtcacat tccccttttt cgtatcctat tttaagcgac ttgcttggca taacggttca
       61 cgttttgctt gatattcccg attgttttcg gatcattgat tcctgtgact tcttgaatca
      121 attgttccaa gttttcatgg atcttcattt catgcaattg acggctttgt tcatcttcag
      181 gatcatagta attaaaaata atccccattg ttgccgtcaa atgtgggcaa gtcaagttat
      241 gttcttgtag ttctctcact ggacgtgtaa atctttcttg ataacctaac ttacgtaatg
      301 gtgtccgagc gacacgtgtg atcgcatcag agatattttt gttttggaag cgatgaatga
      361 ttttttcaat atacgcatga tgctgctctg catcaaaccc ccatttggca atcagcaaac
      421 ttcctgtttc ctgcaaaact gattttaatt gtgccacaac taacgcatct tgcattgctt
      481 catcaatggt ctggtaacct aacaaggcac ctgtataagc gacagtcgca tgaccagtat
      541 tgacactaaa tagtttgcgt tcgatatatg gttctagatc atctacatat aaaacccctt
      601 ttaattggat ttttttattt tttctttggc tatcgtcaat gacccattct ttgaaaggtt
      661 ccacttggac gaataaagga tcttcatggt gttgtaatgg gacgattcga tcgactgctg
      721 catttggaaa gccgatatat ttgtcagtaa atgatgtttc cggaagatat ttatacactt
      781 ctttttctag aaatgctgaa ccgccaatca tattttcaca ggcaatgata tctaaagatg
      841 ttgtgcttcc ttccttttct ctttcttgga taccttttgc aatcaactca gcaataaaag
      901 gtaagatatt cgggccaatg gcggtagtaa ctaaatctgc ttcagcgatt tcttggatta
      961 ctttttctgg atttgtttga ttgttgattc ctttgacatt ttttacagtg attttttctt
     1021 ttgattcatc tgctaattcg atttcgtatt cttttctttc atttaaagca tcaatgatcg
     1081 tgtgattcac atctacgaaa gtaatctcaa atccattagc tgccaaaacc tctccaataa
     1141 atcctcgacc gatatttcct gcaccaaaat gtactgctct catttttctc tctccttaag
     1201 caattgctag attttccgtt acttcttctt tgctgagcgc gtctgctagc tggacaacat
     1261 tgtccatatc actgcaataa agtgcgattt gctgtacaag ttgcaaatgc tcatctccga
     1321 ctcctgcaat accgaacaaa acagtcgcaa tcttttcttc ttcttcggta ccaaaatcta
     1381 caccatctgg tacttgaacg acacagatgc cggatttttt cacatatttt tttgcttcat
     1441 ctgttccatg aggaatcgcg ataaaattcc ccatataaac agataacatt ttgtctctat
     1501 ctaccatggc atcgatatat tcctcatcaa cgcaccctgc ttctacaagc atctgaccgc
     1561 aatagcggat cgcttcttct ttggttttaa aagatcgatt gagctcgatc atgtcgaatg
     1621 gcaaactttg catgctgttt tcctccctta agctgtctgt ttcaaatatt gtgcaatcga
     1681 ttcgtatttt tgcggattga ccatgctgtt gactgataaa tgaatggatt gaggtgcagc
     1741 catctgtact ttttccgcta aactttcttt ggctacgatc agtgaatcag gttcatcttt
     1801 taaatcttgg atattgactt ttcttaacgg gatagttaaa tggttcttgt ctaatatatc
     1861 tttgagcacg gtcatcccca ttgttgctga tccggaacga tcatcgtgga caagtagaat
     1921 ctctttgatt tgtttgatgt ttgctgaaag aaactcttct gctggctcat tctcaatttc
     1981 agaaactgat gttttctgct tgctgcttaa ttcttgaatg atttcatcat atcgaggtga
     2041 attcaaaaag ttttctactg agacaaaacg cgcgctcggt gctttttgtt ttgctctttc
     2101 ttgtagttcg acctgagtaa cgatcaatgt attcgagtca tctgtcagtt ggctgattgc
     2161 ttgattggtc accgtttgag gcaagtctgc ttcctgtact ttttttctca agatcgaagc
     2221 gcccatcgca cttgatccca ttcctgcatc acaagcaaaa atgatttttt gtacgccaga
     2281 gaatgtagct ccttgaggaa taccttgggc acctttggaa gcttgcttag ctgcctttgt
     2341 ctcttgaaca gatttttcga agtcttcatt tgtatctttc gcatccgctt tcaaaattac
     2401 tgcagaaata ccaaacgaaa cagctgcggc aacaatcacg cccaaaataa caggaaggta
     2461 agaattgaac ggtgtcatcg ctagaatcgc aatgatcgac cctggagaag ctggcgctct
     2521 tagtccagca tccatcaatt ggaaaatgaa acttcctgac attcctccgc ctataactga
     2581 taagaataat aacggtttca tcataacgta aggaaaatag atttcatgga tcccgcctaa
     2641 aaagtgaatg atcatggctc ctggtgctga agattttgct gctcctttgc caaacaaagt
     2701 aaaagcaagt aatacgccaa gtcctggacc aggatttgct tcaagcaaga atagaataga
     2761 ttttccagcc tctgctactt gttcagttcc caatggtgtc aaaatcccat gattgattgc
     2821 attattcaaa aataagattt ttgctggttc aataaaaata cttgtcaatg gaatcaaatg
     2881 ggcagataaa atgatctcta caccttttgc cattgcttga gtcaatgcat cgacaactgg
     2941 accaatcgca gaaaaaccta ataacgctaa tccaaaaccg ataagacctg ctgaaaaatt
     3001 attgactagc atttcaaaac cggatttgat ccgtttttgg aatagctgat caaatttctt
     3061 gatggcatat ccaccaagag gtcccatgat catcgcgcct agcatcattg gaatatctgt
     3121 tccgacaatc acacccatcg ttgcaattgc cccaacaact gatccgcggt caccagcaat
     3181 tacttttccg ccagtataac cgatcaataa aggaatcaga taagttagca ttgggcttac
     3241 catggtagcc aaagcctcat ttggtaaata accatctggg ataaatagag ccgttaatac
     3301 accccaggca atcagtgctc caatatttgg catgaccatg cttgataaag tactgcctaa
     3361 tttttgtacc ctcgctttta gcgaaatgtt gtttttggga atcgtccgtt ccattttgtc
     3421 tctccctctc tccttttgat actctaattg tatccgctta ctaaaaagag aaatagtcgt
     3481 actttggcac gaaactttgt gccaaaggtt tgagtatgtc agcaaaataa aaagatttcg
     3541 gtatcagaaa acttcaattt taaataaaac tgttatttta atttttttat aatatgcaaa
     3601 caataacttt tgttagcatt taatttagat gttttccatt ctattatgtt attaaatttt
     3661 atattggagg cctatctatg acattactga tcaaaaacat gaacatgaaa cgtggtaaaa
     3721 aacgtgtgat tcgacaattg aatcttttag tagaatcagg agaagtagct gctttgattg
     3781 ccccgaacgg atttggcaaa acaactttgc tgaatggcat tgcccaactg ctgccaacaa
     3841 cttatgatca attgtccctt cacaacatct ctcctactgc ttatacagat tatcgccggt
     3901 ctttcttttt tctagaatca acgaatcagt tatccgacta tctatcggct gttgattatt
     3961 tgactttgat caaatactat tggcagagca aagtagaatg ccaaactgtt ttccaacgat
     4021 tgaagatgac tgagtacctt actttgccga ttggaaaaat gtctctagga atgaaacaac
     4081 atgtcttgct tgctgcttac ttgatcagtg attcatctgt catgttattc gatgaaccgt
     4141 tgaacggatt agacccagga agtattgata tcttgaatag cctcatcaga caatggcaga
     4201 aagaggggaa gacgattctt ctttcttccc acgctatcgc gaatatccaa tcgatttgtt
     4261 ctaaagcatg ttttctaaaa aatggagaaa tcgtcacaga atcctcaaat atggaagaga
     4321 ttttagctat ttatcgtaaa ttatatgagg tgtaactatg tctttactta catttgaacg
     4381 caatctctta ctaaaaaaca aagtaaactt tttatttcct ctattgttgg tcgtgctctt
     4441 tgcattccca cttttttttg atcataaact tgcttataca gaattcgatg aactgaatca
     4501 taactatgaa gagatgcaac gtttgattga aacgttgaaa gaagatgaga atgagaaaga
     4561 atttgtggaa agtttagaga aaagcaacaa actgatcgaa gcaatcttac atgcaaaaaa
     4621 cacagggaat gtccagcaaa tggtcgaagc aacttatcat tatgaaaaag atatactgga
     4681 tcgactgatt tctggtcaaa gacaaggaat tccaatcatt gaacaacaaa aacgtgtcga
     4741 gttgctccgt tatatgaaag aacatcagat ccaaagatat tctatttttg acttgccagc
     4801 gcatctccct ttagcaaatt actacgaaaa tattttttca ggaatgattt ctagtttttt
     4861 gattttatgt ataaccgcct tgtttctttc ttccatcatc tcttatgaaa aaagaaagca
     4921 ggtaatctct ctagtcaatc tattaccgga tagtatggta aagaaacata gcatccgttt
     4981 taccatttat tatggtgcag ctatgctaag tctcgtgatg ccatttttga tcgtctctat
     5041 actagtaata atcaaaaatg gtttgggaga cttccgttat cctgtaggaa cgatcattgg
     5101 tcaggagata cgaatccttc cgatgtatga gtatcttttc cagagctttc tgttcctgtt
     5161 gttgtgggtg ctctttttaa gtacaatcag ttttcttcta tctgctttat ttgaacattc
     5221 tctagtcaat cttttaggta cacttctttg tcttttctta gcagagtatc gcttgttttc
     5281 ttcgattgga tggatagaat caatcagtca ttacttgcca acttcttatg ttgattttca
     5341 aaatgtgatc ataggaggag atattttctc gccactagct tcagaacagg ttactttcat
     5401 gaatggtatc ttgacacttg gtatatggag tatcgtttta ttattcatag gtatgggcac
     5461 tatctatata aaaaagagct attaaaacac aaaaataata ggggcggtga caaaaacctt
     5521 gtcaccgtcc cctatttcaa ataaacggtt ttctagaata aatagttttc aaaagcttgc
     5581 atccgcagta ataagaacct cccaacaaaa atagctcctc ttatttttat cttggttgaa
     5641 gttattactt gatgcaaggc agcttttttt actgtgaaca ttggttatat atgtccatgt
     5701 tcaaaaatca tttggacttc ttcaatggtt ttcgcatcac tgagcgccat tacttggtcg
     5761 atgtctgaac agaaaaaggc taaatcttgg agcaccgcta actgatgttc tttttttaaa
     5821 gccactacaa ataagagtgt cgcgattttt ggttcctctt ttgttccaaa gtccacacca
     5881 tcaggcactt ggactaaata gattccttct tctactactt cttcatcttt gtcagtccca
     5941 tgtggcaagg ctacgaaatt cccgatatag acactcgctt tttcttgtct ttcgatcatt
     6001 gattggatat atgccggttt gattccccct tgttgggtca tccactggcc gatctcggtc
     6061 aacgcttctt tccaattttg ataggattga tcgagcaata ttttttctgc tggtagctgt
     6121 atcatctttt caccttcatt tctttatttc ttcaatcagg attttcgcta acaactgata
     6181 aacgatttct ttatttcctg agcggaagat ctcggtgttc aagtcattca tgatgattgc
     6241 tccactgatc tttcctaaaa gaatcccgtc tttttcttgg attggcgaag gcgccagcat
     6301 aactaacata cgggacaaat cgactggctg tctatccatc ccttcaatgg tcaacggatg
     6361 tttcaaattg aaaatacaaa agatcggttt agaaatcaat gagctggatg tatggaataa
     6421 ccccatattt gtatgaggaa taccgatagg tgcttgttca tatcgcttaa gcagccggcg
     6481 atgaaccacc tcttcatccg ttactagtcc ttcaggaaaa taaggaaata agctgcgaat
     6541 cgtttcggaa atcgtctcag gattatccag ttcttggata aaaaaaagac cgagtaattg
     6601 attgatttga tccatgaaat gcattgtttc ctcataagta tcttctatca tccactcgtt
     6661 attgctgtta tacgtaattt tttgcctttt tttcttctca atatgcgaaa aagcttcttt
     6721 tagctgcttg acttcatctt ctagcagtag cggggagact aaaatatatt ttccaccgta
     6781 tcctggtaaa atagatgtag aaataacaac atcatattct tcttgtaaat cgattttttt
     6841 taaatcagca atccgataga aatgaatttg ttggatgaag ggaaaatacc ttttcaatcg
     6901 tagttccaac atgctagtag aagctaatcc acttggcgaa aatcctgcaa tactgatatt
     6961 gctttctttc ggacttcgtt ccaatgaatt cgcaaaatga aggaccatat aagcgatctc
     7021 ttcttcggat aatttttttt cttgaaaaat ccgctctact gctttacgga ttgcattcgc
     7081 aacttcctcg tattgaacca tgattttttc taaaatcgga ttggtcagtg tctcttcttg
     7141 taaaacggct cttgaaaaca caccagataa atgagtcaac agcattttgt acaaagtttc
     7201 atcttcataa aaatggacat tcgtctcttc acttacttct tgaatcagtt gttttactaa
     7261 ataagcaagc tgtgtatcga acgtctcttc aaagaaattc tgatcaaatg agttcgtgaa
     7321 gtcatttaat aatcctgcaa aaaagacgat ctcatttact ggataaagtt gttttgtctc
     7381 agaagcgatc cgtgaaaaca atcgtttagc tgtctgtagt aattctttgt ttgccagact
     7441 attatatgtc tcatttgtca gtaaatgatt tttagtcaca cgctccatcg tcaatgtcag
     7501 catcaaaacc aaatgttcaa gctttctatc acttagattg gtcttttttt ggatcaattc
     7561 ttccccgatt actttgcgtg caagagatag atgttgcgga tcaacgaatc gaaaaaaata
     7621 attcagtgat gcatcgaaag aaatactatg aaaaagttca tattcattga tttctaattc
     7681 taatacattc gctgtcagaa gccggcggta tttttccggt ccttcgatct cgtatccctg
     7741 gttccgaatg atttccaaag gcagttggcg aatactttct tccaactgtt tgatatctgc
     7801 ataaaaagtg gtattactga tgcgatatga ttccatgaaa tgagcaagac tcaacggctc
     7861 tgtcgtcagt aacaactcgg ctaaaatcgc gtgttgtcgt ttagcagtgg ataagtcatt
     7921 acttttccca tctgtatttt cttttaattg ctctaatact tcttctgatg cctcaatgac
     7981 aaatgagcct ctagctgctt ttttcaaaga agcatttacc gatttcaatg agacttcaag
     8041 attatctaat tcgcggtaaa tagtccgttt actgaccttc aataaattca tcattttttg
     8101 gatagataca ggtgacgggc tactgagaaa ttctgctaat aattgttttt ctctgggcga
     8161 taaatacatc ttatatttct cccttcacga gtttttcgta ttcattcgta gctacccaat
     8221 gttcaactag aactagccga gggatctttt cttctagctg ttcagttaca gctagttctc
     8281 tttgtgcgat ataaatcgtt tcttcttgga ttttgatttt ttctattgat tcttttgaga
     8341 taacaaactg ttttttcgct ttttttgcta attgacttaa aatcgtcatg cccatagttt
     8401 gtgagccgcg tctgttgttt ttatacaaaa atacaatatt agcaggcgac tttttttgtt
     8461 ctgtgatatc tgctatctct tttttttctt cccgctttgg aaattggatc gcttctttta
     8521 ttcgtgacag caagatatct gtttccaaaa agtgatccag tacatagatt tgactttgag
     8581 gtactttttc tcttgcaaga cccgctagtt catgttgcac gataaccagc tgatttactt
     8641 ggttattcaa ttcatagata gagctgtatc cgacagaaag atcgattttt tctgctgcta
     8701 atttcttttt cagcagggcg gctcccatag cacttgatcc catacctgag tcgcaagcaa
     8761 aaatgacgga atgaatgata gatggttcca agatttctcc cctttctttg accgttttgt
     8821 tctctttttc tttatttggt aatttcaata gtatcgctgc acaaatgaag gtcacaaatg
     8881 tactgattgc tatccctata cttaccccag ctagttgtgg ataaggcgca ttggcaaaaa
     8941 tcacgagtag tgacccaggc gatacaggtg cagctaatcc cgtatctaac cattgaaata
     9001 tcaacgtgcc gcttgcccca ccagccatta cagctaaaaa tagatacggt cgcagcaaga
     9061 caaatggaaa atagatttcg tgaatcccgc caatcgcatg gatcatcaat gctccactcg
     9121 catttacttt ttcctgtttt tctgcaaaac aaaggataga aagtaagatc cctaaaccag
     9181 gtccaggatt tgcctccatc aaaaataagg aagagtaccc gactgtttgt gagaattcga
     9241 ttcctaaagg tgtcaataca ccatgattga ttgcattatt gaaaaatagt acttttagag
     9301 gttctaagat cagactgaca agtggtaaca gattcattgt gactagccaa gacacaacgg
     9361 aggcgagcaa caagtgaaca ctatttaaaa ctggttcaat aaaaacgatt ccaattatac
     9421 aaataacgct accagtcagt cctactagta ggttgctgac aagcatttca tatccagcct
     9481 tgaatttcac gtggaatcta gaaacaaatt gttcatatac gatgccggca agcggcccga
     9541 taatcatcgc tcctacgatt tgtggagcat tggaacgagc gatcaaccct aaagaagcaa
     9601 ttgcaccggc gactgcccct ttttgtcccc ctaccgattt cccgcctgaa taactgatca
     9661 agatgggtaa aagcagctgg atcatccaat ggcatacctc ttctaatgtg gtgtgcaaat
     9721 cgccagacac accttttgtt gctaatgaca agattcccca agcgataaac agacttagat
     9781 ttggcatgat catttcattt aagtgatgtc cagcttttct cagttgtttc atagacacgc
     9841 gactgctgtt gtttgctttc catttgtatt ccatcttttt caccatactt ctatttattg
     9901 atggacggat tttatgtaga aaaagcagca aacgaaaaac cgtctgctgc tttcagctat
     9961 ctgggaacta tttgcctggt tcagtcgcaa tgattgctag ttcaccttgg accgcccact
    10021 cagaaacgcc atttggaata atacagcttg ttcctttttc taatggatat gtttttccgt
    10081 caacgacaag gtctcctgct ccttcgatta ccgtcatcaa tgtataaggg gcttgttttt
    10141 tgaaagatgt gattccttta atatcccact catatacatt gaaaaactca gtttctaaat
    10201 aagtgacgat cgatgaattc ccttttcgga cttctttgat ttgtaattct ggtgttttcg
    10261 cagggacagt tgttacatcg atcgattgtt ggatatgcag ctctcttgtt tgtccttgcg
    10321 catcttttct gtcgtagtca tacacgcggt aagtcgtgtc agaactttgc tgtgtttcta
    10381 aaatcatgat tcctttacca atagcatgga tcgttccgct tggtacataa aagaaatcac
    10441 cttttttaac tggtactttc ttcaaaaggt catcccagcg cccatctttg atcatctcag
    10501 ctagttcttc tcttgtcttt gcatgatgac catagatgat ttctgcacca ggttcagcat
    10561 cgatgatata ccagcattca gtttttccta attcaccttc atgtttcatc ccataggcat
    10621 catctggatg tacttgtaca gatagatcat cttctgcgtc taagatcttg atcaataatg
    10681 gaaacactgg ttctgaagga tggccaaaca attccttgtg ttctgaccat agttcatcta
    10741 atcttttccc tttgaattcg ccattttcta ctacactgac accatgagga tgcgcactga
    10801 tcgcccaatc ttctccgatt ttatcgttag ggatatcaaa accaaagact gaacgtaaac
    10861 gacttcctcc ccaaattttt tcttgaaaaa ctggttttaa aaacataggt tcctgcataa
    10921 ttcaaactcc tctcattttt catagctatt atagcattat ttcggtaata ccgctttctc
    10981 tttcaaaaag ttttcaacaa tttcatctcg acatatgaga tactccgcac gatgatcgac
    11041 aggatgtacg cgatttgaca aaaatatcaa cgcttcttgt gtttttatat caatcaagaa
    11101 aaacgtacct gtataaccag tatgaaaaag aatcgcatga ccgtcttcta catccttttt
    11161 taagtcccat cctaaagaac gatagccatt ttttttcggt gtttgatctt gcagtaaaga
    11221 atcaatcgtc tcttcttcta aaaaacgacc attacggatt tttcccttgt tcaaatacat
    11281 ttggacaaaa gtaatgctgt ctttcaaatc aataaagaga cccgcgttac ctgcatgttc
    11341 agccaaaact cttgctttcg gatcatgagt ttgtccacgc aaaacctcac ccgaaggcag
    11401 ttgttctgtg ggaacgattt ttttggaagg aaaaggaaga aataagctgt tcatcattcc
    11461 taatggttcc aatacttgtt cttgaaagat ttccactgca gtttcttggt aaatttcctc
    11521 tagcatcaac ccaagaagaa tcgtaccggc atctgtatat tttacttctt ttcctagttg
    11581 ctcacctggt tctagcttca aataagcggc gatcaattct tcctgagata gcttatctct
    11641 gtttggtatc catgttttga tatcagaagt atgtgtcagt aaatgacgca aagtgattgt
    11701 ctgattttca aattcaggaa gatatttatg aagcggttga tcgagttcta tctttccatc
    11761 ttctaataga cgtaaaacta atgaagtggt tgcaacgact ttcgtcagtg aggctgcatc
    11821 aaacaacaga tcttctgtca tcagctgctt ttcaggaacg acttgggcaa agccggctgt
    11881 aaatgtttct acttgatttt cttttatgaa ggcgactgct acacctggat aaaatccttc
    11941 ttccatcttt ttcaaaataa gctgtttcgt gtgtggatac atctttttct cctgcttttc
    12001 tatcagattc ttacagtcca agcttaaagc ttaatctatg aggaatcaac cttttttaca
    12061 caaaaaaact gcgcgaaaaa ccgcgcagtt tcccccggta tactattttc tctttctatc
    12121 tctagaataa gcgatagtat gaccgttcat aaaatgacga gacatgcgac aatcgttaag
    12181 aaagaaaaag ttaagcgtcg catcgaatgt cagttttatt cttttctttt ggctcactga
    12241 cgaaccacca ttcgattcct atagcatcat agattgtgag tactgtgtgc tttttgtcta
    12301 agaagaaatc aactgatagt tgctgcaagt gagcaatcag tccttccatg ctttcagtat
    12361 cgatcatgaa tttcagaaaa tcaagtccaa tcacttcatc tgtaggcaaa tcaattgcgc
    12421 ctccgctagc ttctgtcaaa ccaacatgga aatctccttc attcaataca tggatgccct
    12481 cttcttcatt atgtaccttc aaaccaagta catgattaag aaattggcgt tcttttgtta
    12541 agttagtaac atttaaatgg attttttcaa aataagaacc tgttgatagt ttactgaatt
    12601 tttcttcaga ttttcttaat aatgcttctt gatccattgt ttctggaatt tctctttggt
    12661 cgttcttatt ctcttcatgg taaatttcca attcattctc ttctgggtct acgagcaaaa
    12721 tccccatacg tccatgatct tctaaagctt ctttgattgg ataattcatt ttacggatac
    12781 gacaaaggat atctgccatt tcttctgtgc tagggataat caatgaaaaa cggttcagct
    12841 ttttgatcac ccccatgtga tcattcgctc taggactttc ttccagcagc aacatttcac
    12901 ttccagcttc ttctgttcct aaaatggcta attcgttttc ttctcttttc aaatcaaagc
    12961 caacaacgtt cttgtaaaaa tcaatcattt tatctcggtc tttcacccga atggctacac
    13021 tttttaaata ggtggcagcc cccaattgaa aatctttcat atttcttgct cccctctacg
    13081 tcaagcttta ttttaacgat ggattcttct tctttcaagc aataactttg attttgttaa
    13141 aaataatgtg agaaaattcg cattttgctt gttttttgta aattaatgag taaaaactct
    13201 tataaaaaga ggttgtgaaa aagctgttct gcatcaagta ataagaacag ccaaacaaaa
    13261 atagctcctc ctatttttcg tttggttggg cttattaccg cagatgcaag cttttgaaca
    13321 ccgtttaagg aagaggttgt aaaatcagcg gtttgacctg agtattagag atacaaaacg
    13381 gaaaaatagc ttctcatatt ttgccgtttt ttgagctaat accgaaggac agctgattga
    13441 acaccgttta ttcggaaaaa gggactgtga cacgactttt gtcacagccc ctaaaaaaaa
    13501 taataaattt gtactattct atgaagcttc ttcttcagaa aactgactgt tataaagatc
    13561 tgcatagaat ccgtcttttt ccatcaatgt atcgtggttt cctgtttcta cgatcgaacc
    13621 atgattcatc acaataatgt tgtctgcatc gcgtattgta gacaagcggt gagcaacaac
    13681 gaaacttgtc cggttttcca ataggcggtt catcgctttt tgaatcagta tttctgttcg
    13741 tgtatctaca ctagatgttg cttcatcaag gatcaggaca tctggatttg ctaagaatgc
    13801 ccgcgcaatt gtgattagct gacgctgccc ttgcgaaata ttgcttgcat cctcgtttaa
    13861 tacagtgtta tatccttctg gcaatttacg aacaaaatcg tctacgtgag cagctttagc
    13921 agcctcaatg atttgttcgt ctgttgcatc gtcgttccca tatttgatat tgtcatagat
    13981 acttcctgta aacagccaag tatcttgcag taccattgaa aactgtgcgc gcaattcatc
    14041 acgtgaaaga tcacgggtat ctacaccatc gtaacgaata ctacctccag aaacatcgta
    14101 gaaccgttca agaaggttga tcagtgtcgt cttccctgca ccagtcggac ctacaatagc
    14161 gaccatctca ccaggtttga catccaagtt aaaatcagtc attagcaagt tatctggtgt
    14221 gtagccgaat tgaacatttt caaaacgaac tttgtatggt gtatcttttt cttctggaag
    14281 gccagatttt tcattggaca tctcttcttc atccaacact tcaaataccc gttcggctga
    14341 agcaactgtt gattggatag tgttcatcaa acttgcaagt tgagtgattg gttgagagaa
    14401 ctgtgttgta tattgcaaga acgcttgaac atcccctaat gtcatgttcc catttgcaac
    14461 ttttactccg cctagtacag cgacgaacac atagcctaga tttttaacga aattcatcaa
    14521 cggcatgatg attgccgaaa taaattgagc tttccaacca gcggcataca gattctgatt
    14581 ttctttttca aatacttcct ggtctttttc agtatgattg aatgttttaa caatggtatg
    14641 accaccatat gtttcttcta cttggttatt caataaacct aaactttttt gctgatctgc
    14701 gaaaaacact tgtgatttcg gcgctacgat catcacgatg atcaagctta aagggatcat
    14761 caataacgcg actaacgtca attgccagct gattgaaagc atcatcacga tgaccccaat
    14821 caatgtgact gcactagtta caaattgagt caaattttgc tgcagcgtgc ttgcgatgtt
    14881 atccatatca ttgattgctc gagacatgat atccccattt gtatgtgtat cataatagga
    14941 aatcggtaca cgattcattt tggcttccaa atcatgacgt aattcataaa ctgttcgttg
    15001 ggaaacacgt gtcataataa attgctgtaa aaagttgaat acagcagaaa tcaaatacat
    15061 cacgattact gtcgcaatga tctgcgcgat tttatcaaag tctatcggaa attttgttac
    15121 ttgttggcct tgttgcatct gttgagcgcc agccattaca cctttgaata tttctgtggt
    15181 tgctttcccc aagacctttg gtgtttggat ttggaagatc acggctgcta ttgctagtac
    15241 aaatacagcg atgatcgcaa ccatacgttt agacatatag ccaaacaatc ggcgtaccgt
    15301 tccccagaaa tttttaggct tttcaccttt tacaccgaga tttctgccag gtccaccatg
    15361 tcctggaccg cctgctggtc catgagaggg acgtgtgttt gttttactca tgctaagtcc
    15421 tcctctctta gttgtgaatg aacgatttct tgataagttt cgtttgttgc taatagttct
    15481 tcatgtgttc ctttgccaac cagttttcct tcatctagca caaggatcat atctgcatcc
    15541 attactgtac ttactcgttg agcaactaaa acggtgatac tttctttcat attcgttttc
    15601 aatgcttggc gaagattggc atccgttttg aaatccaacg ctgagaagga atcatcaaac
    15661 acatagatat ctgctgtttt gactaatgcc cgtgcaattg ccagacgctg tctttgtcca
    15721 ccagagaagt ttcctcctcc ttgttccaca tggctatcta agccatcttc catctctgaa
    15781 acaaaatctt tagcttgtgc gatttctaat gctttccaaa tcatttcatc tgttgcatcc
    15841 ggcttaccat actgcatatt gtctcggatc gttccagaga acagaactgc tttttgagga
    15901 acaaagccca ttaattctcg caaattatat tgagtcatgt cactaatgtc cgttccattt
    15961 atttggatcg aaccagattc gatgtcatat aatcgaggga tcaaattgac taaggtcgtt
    16021 ttacctgaac cagtcccccc tatgattgcg acgatctcgc ccgacttggc ttggaaatca
    16081 atatcttcca atgcaagttt ttctgctcca tgatatcgat aattgacatg attgaaggcc
    16141 aatgttgcgt tttttccagc aaaagaaact gttttaggat tttcagggtc atggataccg
    16201 attttttcat ccaacacttc attaatacgg tcagctgaag cttgtgcacg aggcaccatg
    16261 ataaaaatag cggaaagcat cataaagctg atcaagatct gcatcgcata agtcataaat
    16321 gcaatcagat taccgacttc caaagtcata tctgctatgt agtgtccacc aaaccaagtg
    16381 attgctacgt ttgtaccgct catgatcaat gtcatcattg gcatcatcaa tgcgacgatc
    16441 gtattaactt tgattgccgt atttgtataa tctttattcg ctaaatcaaa acgattttct
    16501 tcaaagcgtg tcttatcgaa tgctcgaatc actctgaccc ctgtcaatcc ttctcgaaat
    16561 accaggttta aacggtcagt tttcttttgc atacttttga ataatggaac agcaaagtac
    16621 atgattccgc cgatcaaaat aatcattact gggatgacaa ctaagaagat ttttgtcaac
    16681 tgatgatctt tttgataagc aagtatgctc gcgccaatca acgtgattgg tgcattgatc
    16741 atcatacgta aaaacatttg tgtaaccatt tggatttgag tcacatcatt ggtcgttctt
    16801 gtgatcaaag aggccgttcc atatttatca aacgcatctt ttgtcagact ctctgatttt
    16861 ttatagacat ccgtacgcaa ttgcttaccc agtttctgtg attcacgagt agcaaaaaat
    16921 gtatttgcga tagcagctaa gatactgatc aaagaaaagc cgatcatcac aaaacctgta
    16981 tgccaaatat aatcgacatc tccttgcgct acccctttat caataatatt ggaggtaagt
    17041 gtcggcaaat ataaatctgc cagtacttgg atcaccatga aaactgcagc tgcaattgct
    17101 gaagtcaagg agatccgttt tacgattttt agcatctagt tcccttcttt ctatttatat
    17161 actccgtttt tcagccaaga aagtttcaaa ttgaattcac tgattgcttg ctctactgag
    17221 taatcttcag atgttgaaaa taagcgatac gaatgcgcaa ttgttgtaaa tacagtattc
    17281 atcactaatt ggatcagtaa ttccaattca tgatcatttt ttactttcag cttcgtccga
    17341 tcgatcaatt caagcaactc ttttgcatct tgtttgtgtt ctttatgtgg atcgtgaggc
    17401 gttttttggc gactcgtatg aggagcgact tttcttgagg cacggtaatc catgttcata
    17461 aacaagttac gataaaaaga agcattctcg ccttccaaaa tctcataaat cattttagaa
    17521 aaataggatt cgaatccttt gaatagatca ccatcggatt ctttcattag ttgtaacata
    17581 tctttagagc tgtcacgtct cattgtctca aagtaataaa aataaagatc ctctttatct
    17641 tcaaaatact gatagaaact gcctctaggg atatccgcta atttgacgat attagcaatc
    17701 gaagcttctt ttaagggtac tctagaaaac tcgattctcg cagcctcgat tagtcttttt
    17761 tgtttttctt taggcaaatg gaaaaatgtc tgtgtcggca tatggattct cctttctttc
    17821 aagatgacac attgtcatat gacgctatgt cattataaca cctcctacct gttttgcaag
    17881 acatttgcat agtttttttc attatcatcc gatacattaa aaaagtgata agaggaatcc
    17941 ttccatttga attaatgaga gttgcccatt aaaatagcca catactaaac aaaggatgag
    18001 ataaatggcc caatcaatta gtgtccaaga cttcaataaa ttgcctctcg atgctgacag
    18061 catcgtttta gatacaagaa ataccttaga ttacacaaaa aaccacttaa gccactcgct
    18121 ttctatcccc gctgctatct tgccaagaca attaaaaaat ctagatccaa agaaacttta
    18181 ttatattcta agctatacag gcaggcgatc agaagcatta gccgatcaac ttactgctaa
    18241 tggttttcgt gctgtttata tcattggtgg gatggaagct cttcagtcga gtgttgcttg
    18301 aatattgctt gttagattca aaagaggctg tgacaaaaaa tcgtgtcaca gcctctttta
    18361 taccctgtct ttttttagtt atttattctt caaccttatc aatatgactt gatagccttt
    18421 tctttttcga gtaaactgat tttgttagtc atagttataa caatcatagc aataaaagga
    18481 gaaggtaagc atatggcatc tgttttagta gtcaaaggtc atcccctaac tgcagaagaa
    18541 tcgcgcacag ttaaggcatt gacgtctttt ttaacaagtt ataaagaaaa tcatccagat
    18601 gatgaagtga ctgttttgga actttatcgc gatgatattc cggaaatcga tgaagaatta
    18661 ttatcaggtt gggaagcatt acgtgctggt gcagaattca cttccttatc agaaagtcaa
    18721 caacaaaaaa ttgctcgttt caatgaattg actgatcagt tccttgctgc tgataaaatc
    18781 gttatcgcaa acgccttatg gaatctaaat atcccaacaa aattaaaagc ttggttcgat
    18841 accgtcaatg tagctggaaa aacgttccgc tatacagaaa acgggccaga aggattagtg
    18901 acaggtaaaa aagcactgca catccaatct aatggcggtg tatacaacgg acaagacttt
    18961 gcctctcaat atgtaaaagg tattttaaac tttgtaggca tcgatcaagt agatcagcta
    19021 ttcatcgaag gaatcgatta tgaccctgac cgtgcagatg agttgatgca aaatgcatta
    19081 gataaagctg ctgctttagg taaatcattt taaaaattga gataaatgca tacaaaagag
    19141 acagaggaaa aaatcttcaa agcagcattt gtgccttgga
//
LOCUS       DAFEOM010000051        18895 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.052, whole genome shotgun sequence.
ACCESSION   DAFEOM010000051
VERSION     DAFEOM010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.052"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(21..251)
                     /locus_tag="LP524_001937"
     CDS             complement(21..251)
                     /locus_tag="LP524_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234970.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(687..1253)
                     /locus_tag="LP524_001938"
     CDS             complement(687..1253)
                     /locus_tag="LP524_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBL2234971.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1253..2209)
                     /locus_tag="LP524_001939"
     CDS             complement(1253..2209)
                     /locus_tag="LP524_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBL2234972.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2209..2865)
                     /locus_tag="LP524_001940"
     CDS             complement(2209..2865)
                     /locus_tag="LP524_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBL2234973.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3235..5253
                     /locus_tag="LP524_001941"
     CDS             3235..5253
                     /locus_tag="LP524_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL2234974.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5323..5529
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5680..8094
                     /locus_tag="LP524_001942"
     CDS             5680..8094
                     /locus_tag="LP524_001942"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBL2234975.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8442..9995
                     /locus_tag="LP524_001943"
     CDS             8442..9995
                     /locus_tag="LP524_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBL2234976.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10376..12160)
                     /locus_tag="LP524_001944"
     CDS             complement(10376..12160)
                     /locus_tag="LP524_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2234977.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12175..13896)
                     /locus_tag="LP524_001945"
     CDS             complement(12175..13896)
                     /locus_tag="LP524_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2234978.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13996..14757
                     /locus_tag="LP524_001946"
     CDS             13996..14757
                     /locus_tag="LP524_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL2234979.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15724..15992
                     /locus_tag="LP524_001947"
                     /pseudo
     CDS             15724..15992
                     /locus_tag="LP524_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16664..>18895
                     /locus_tag="LP524_001948"
     CDS             16664..>18895
                     /locus_tag="LP524_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234980.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         6205 a         3353 c         3786 g         5551 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      541 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      601 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      661 tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata taaaataggc
      721 ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc ctgtggcagt
      781 ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg accatagtag
      841 acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc catagctgtg
      901 cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata ccctagattg
      961 aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt ttcgtggcct
     1021 tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc acgagtagca
     1081 tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc gatgtgttct
     1141 gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg atcccctggt
     1201 tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa catcagacgc
     1261 tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga agttcttcat
     1321 caattgcatt caagacttcc cattttttca agctccacat tggtccaatc aaggaatccc
     1381 acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt tccagctggt
     1441 cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct tcatgatagt
     1501 cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt ccttggatat
     1561 ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt tcgcctggta
     1621 atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta gccacgccat
     1681 ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg
     1741 tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca aggtaatcaa
     1801 ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg acacctggta
     1861 aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa
     1921 aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc atcatgtcga
     1981 tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg tctcccgaac
     2041 cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga ttcggacagt
     2101 caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt
     2161 aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt
     2221 ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat aagtcaggca
     2281 cagccaagaa agactgagta agtagccgcc gatgatatca gtaggaaaat gaacgcccag
     2341 ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt gaagcaatgt
     2401 gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac cgtaaagaat
     2461 catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta ctaagtgcgc
     2521 caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga ttccagcaat
     2581 tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat accacatcca
     2641 taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt tagtcataaa
     2701 gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa accactgatc
     2761 gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga aaaacaggac
     2821 aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat tcctcctttg
     2881 aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag agataaaacc
     2941 aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact gtaatcggaa
     3001 agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat
     3061 ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag ttgttcatta
     3121 cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt ttaataaata
     3181 ttaagagaag ttataattat taataataga taaattgaag ttggaggata gaagatggaa
     3241 aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg aaaagcaaaa
     3301 cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc cccattagta
     3361 cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag aatcaataca
     3421 gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc caacgatcta
     3481 tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag tagtgcgtta
     3541 tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg acaaaccgtc
     3601 tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga gcctttccga
     3661 caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag aaatacttca
     3721 tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc ctatacagat
     3781 gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg caaattaaat
     3841 aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg aaacgcaggc
     3901 ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg cggttcaagc
     3961 aattctggta atacaaactc aggttcagca acttcgacca cttatactgt ccaatctggt
     4021 gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt gatccaatgg
     4081 aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa aggtagccag
     4141 gaaaatggct cttcgacgaa taattccggt aacaatacca actcttcagg aaatgctggt
     4201 acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg tgattctgtt
     4261 tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg gaacaatatc
     4321 aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg ttcttctaat
     4381 gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc gtccagctca
     4441 agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga ctctgtttgg
     4501 agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa caatatccaa
     4561 aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc aacaaacagc
     4621 acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt taatactggc
     4681 agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg gagcgtggca
     4741 aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa aaacaacttc
     4801 atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac aaatacaggt
     4861 tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac aagtacatca
     4921 tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac
     4981 aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa taatttcatc
     5041 tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca
     5101 gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag tgttgctgac
     5161 agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa caactttatt
     5221 tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa ataacgattg
     5281 aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac ttgaaaagga
     5341 agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg cacggatgct
     5401 tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg gcggtgacgg
     5461 ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg cgtagattac
     5521 gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg aagtttgaga
     5581 aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt tatatttata
     5641 atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa tcataaagaa
     5701 attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac ccatgatgat
     5761 cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg tcaagggcta
     5821 catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt gaaaagaagt
     5881 caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa
     5941 tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat cgaaacattc
     6001 cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt gaatacaact
     6061 gatccggagt attataaatg gacacaatgg atctttacga agctttatga aaaaggtcta
     6121 gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt tatttcaaat
     6181 gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg
     6241 cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga tctagaatta
     6301 gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg ttcagtagga
     6361 gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt tacgacacga
     6421 ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga tcttgttaga
     6481 gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga gacagctaaa
     6541 aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt tactggtgct
     6601 tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga ttatgtatta
     6661 tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg tgactttgaa
     6721 ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga tgttgaaaaa
     6781 gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg catgaacaaa
     6841 caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa
     6901 gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg tgaaccaatc
     6961 ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga gctaccatta
     7021 cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc attagcaaat
     7081 atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg
     7141 aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat cgaccctcat
     7201 aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac
     7261 attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg gcataaattc
     7321 ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata caatcaagga
     7381 atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct
     7441 gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat gttcatgggg
     7501 ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg taaattcttg
     7561 gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg tatcactaca
     7621 ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt gacagaagac
     7681 tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat caatgaagcc
     7741 tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact actcgcaccg
     7801 attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg tggtatttct
     7861 tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt agaagttgtt
     7921 ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat
     7981 gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga aggcaaaaca
     8041 gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa ttaattggct
     8101 ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa tgaacttcat
     8161 tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa aggttttgat
     8221 tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt aagagatctc
     8281 tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa actacctgaa
     8341 attgagagta cataccaaat tcataaattt tttattagtg ggactagtga ttgtatggat
     8401 tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa gaactattta
     8461 ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa
     8521 gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt ttcggagctg
     8581 tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg atgcaaaaat
     8641 taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt gtgcttattg
     8701 tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc gatcaatggc
     8761 ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat gatgactatc
     8821 tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt gattgccagt
     8881 tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa tcaaaattcg
     8941 gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa aaaagccagt
     9001 cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt tttttcctca
     9061 ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg tcttctcata
     9121 taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata caaaagacaa
     9181 atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa aagataaatg
     9241 atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt ggtgctaatt
     9301 gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg
     9361 ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc gatttctcat
     9421 ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat aatggtaaaa
     9481 catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga aaagcggtat
     9541 tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct gcagaaactt
     9601 tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga agtacaatga
     9661 tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt gtttcgacac
     9721 gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca gttctgtcga
     9781 ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat gaagttactc
     9841 aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg
     9901 cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca tggcaattcg
     9961 ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg agcctgacgt
    10021 tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg ttgaatagtg
    10081 taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta tagcttctcg
    10141 tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg tttattcgta
    10201 aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa ggagctatcc
    10261 aaaaagtatc attagtatta ttctttgtgt ttctagtggc accactagta catttaaaaa
    10321 agctatcgat catggactaa tcatacatga tcgatagctt tttagataat gtttgttacg
    10381 ctatctgaaa ttgactatta taaagactgg cataaagcgt tggtgattgg agtagttcag
    10441 aatgtgttcc tttttcgacg atatcgccat tcttcattac aaggatcatg tctgcatttt
    10501 caatcgttga caaacggtgt gcaatcacaa aacttgtcct atcttttgtc acagcttcca
    10561 ttgcttgctg aatcaatgct tctgttcgtg tatctacgct agaagttgct tcatctaaaa
    10621 taacgacagg aggattcgct agaatgactc gagcaattgt caataattgt tgctgtcctt
    10681 gagataacgt gccgttttcg ctagaaatga tcgtatcata tccgtctggc agtgttcgaa
    10741 taaagtgatc acattgtgcg atttcagccg cttcgataat ttgttctctt gtggcatcca
    10801 ttttcccgta ggcgatatta tcagctaccg ttccttcaaa cagccaggta ttttgtaaaa
    10861 ccatcccaaa agaacgacgt agttctccac gtgaaagctt ggtaatatca agaccgtcga
    10921 attcgatctt tcctttattc agttcataaa aacgcatcag cagattgact aaagtcgttt
    10981 ttcctgcacc agttggacca acgatcgcta ccgtttgttt tggctttact gaaaaatcga
    11041 cttgattcat caatatttta tctgatgtgt aaccaaactg tacatttttg aaagctattt
    11101 ttcctttcgg ttgttctaaa agtcttgcat ctggaaggtc tggaatttca tctgtttcat
    11161 ctaatatctc aaatatccta tcgattgctg ccattgcgga ttggatcgag ttgatcacat
    11221 aagaagcggt agaaattggt tcagaaatct gattgatgta ctgaagataa gcttgtaaga
    11281 agccgatcgt cacaccacct gatagaacaa gcatagcacc gaataccgca cttatgatga
    11341 aggcgagttg attaatgaaa cgaatggccg gatagatagc acagttcata aattgagcac
    11401 gtttaaaagc gttgtattgc tttttattta cttcgtgtat agcagcttct gcatgagctt
    11461 gctgattgaa tgttttgacc actagattac cagctaagat ttcttccatt cgattattta
    11521 aaacacccag ttcatactga ttctgttcag aaatctgttt gtttttgttc gcaatcttag
    11581 ttgtaacaat tgaactgcca acgatcagca ataagacgag caaagtaagc ttgctttcga
    11641 tataaaacag catcagacca gcagcagcta ttgttacgac agaagtaaaa aattgattga
    11701 tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga ctaatgatgt
    11761 cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt tttgtgattt
    11821 cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt tcctgaataa
    11881 atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg aacaacaatg
    11941 cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt tctgataaga
    12001 gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg ttcccgaata
    12061 aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt ttccaaaaac
    12121 gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt ctttctatat
    12181 tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt cttcatgagt
    12241 accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt gttggattgt
    12301 actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat gaagggctgc
    12361 tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga atacatagat
    12421 ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc cccctgaata
    12481 attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt ccactagttc
    12541 ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat attcatttcc
    12601 catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag ggacataact
    12661 gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac caagggttac
    12721 ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac tttttccaga
    12781 tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat caatatcttc
    12841 tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag cagctgcatt
    12901 tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa tttcgatctc
    12961 tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga taatgaccaa
    13021 taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga ctacagcagt
    13081 cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc acaaaataga
    13141 agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa atattcggtt
    13201 cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct tttcttcata
    13261 tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc tcaatgtgat
    13321 ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc gtttcatcag
    13381 tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg ttagtaatag
    13441 agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg gtaagatcat
    13501 ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca ttcttgtcaa
    13561 aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga atttacggaa
    13621 atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa agtaactaga
    13681 ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc caattttttt
    13741 tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta ctaattttgg
    13801 tacacctaat gtaccggcga tttctaaaca gatcatcaca aaggtaagga gagcgagtcc
    13861 tttgtttttc tgtaagaatg tttttaagat agacatatta ttcctccttt tttcctgata
    13921 cttttcgatt ctataccttc aagtaacttg aaggtcaata gaaaaaagaa taaactgaaa
    13981 aagaggtgag aaaaaatgaa aaaaatgtat acgattggcc aagtatcgga actatttgat
    14041 ttgccaaaat caacgatccg ctattgggat gaacaaggat taatccgttc aagtagacaa
    14101 gaagagaatg attatcgatt gtttgatatc gatgatattt ttatgcttta tgatatcgtt
    14161 ttttatagaa aattagatat accgatcaaa cagatgaaga atttatatgg aaaaacattg
    14221 acggagttat atgagacact tgatgaaaca gaaagaagaa tccataaaga actggtggtg
    14281 atgaaacaaa aacaaaaaga gataagggag caaaaaaaac agttgaaatt gatgatcgat
    14341 acaaacgagg aagaatttcc tgtagaagag atcccttttg actgtatgat ctcaacggag
    14401 tttgaagata ttgtggaaat caaaaaattt ctacctaact attcaagctt tgggatgatg
    14461 agtatgccag cttccagttc acagacgatg tatggatttt ttatcgaccc tagtgaagta
    14521 catttattta ctcaagacgt gatttgggag aaaaaagata cggctgttta tcgtcgattc
    14581 ttattgaaat ccgagatgaa ccatgcggaa cgtaataata ttttagaaat cagagaaaga
    14641 atgtatgaaa aaggatggaa aacaggagaa gtgatcggcc agtatctatt gacgaatacg
    14701 gatgaaaaca atatcagaac tgaatattat catgcatgga tagaaatgaa aaaataggaa
    14761 atatgtgttt aagataactc gaaaatgatg gttgttttag ttataaagaa aaattttctg
    14821 gttcgattac gggtttaatg tgggggatta ttagtcgtgg atagatagaa ttttagtttt
    14881 ttagaggaaa tatgtcttta aaaatggaat ggttgcaatt cttagcgata tatacgatgt
    14941 aattgtcctc ttaaaaaagt atggaaaaat aattgtattg ggtgttatgt ataattcatg
    15001 gtgaactgag actaatgaga agaaaaattc atattgttat gagtgagatt gtatcagatg
    15061 agaatgataa attaaatagg tgcataaaag cattacactt aaaatacatt gttgcttaca
    15121 ttattacaat atgttaataa ggcaaaaata aatagatgga ttgaataaac atatttttta
    15181 atcaaatagt ccctactgtg actttcctca gctatcggtg atactaccga tagcattttt
    15241 ttgcttataa aataattata agtatttaga gatttttctt ctaacattct tcaaacaaga
    15301 aaaatcacaa aggttattgt tatttttata tattcttttt aagaatgatt gtttttttat
    15361 agaaatttta aaaaataaaa cacgttattg atggatagtt agtttgttat ttggtaatat
    15421 tatagaagtt gactggtagt gatttatttt tcgtcgtttt ataaaaatgt tattttttgg
    15481 aaaaattcac aaatatgtaa cgaatgtttt tctggaattt gctgtgttaa aaagcagtat
    15541 gactaatata aatactattg tcatttatgc aattttttat ggatagtaat tttagagggg
    15601 aatcaatgaa ggaagaatag aagggaaaaa tagttgcaaa tacgccagtt agcaaaaata
    15661 gcaatacatc ttcaagcttt aaaattcgag agaatcatct tggtagtgga atcaactaca
    15721 acaaaaggat taaaccaatt aatagataga attaccaatg gaaaaataga aaaggtagtt
    15781 attctataca aaagttgttt aattcgcttc ggttatgaac ttagtgaaac cctttgcaat
    15841 atatggtaca gaatatagaa attattgaca ttacagaaaa tgatgaagat ttaatacaga
    15901 ttgtcactat ttgtagatac cttttacaag gaaaccgagc caataaagct aagaagatga
    15961 ttaaggagtt gatggaagac gatacaagtt aagaaagtga aactacgccc aaataaagag
    16021 caaaaagagt aattggacgt ggaaaatgca agaaatgaac catcgattcg gtggacagtt
    16081 tattagtaat gacggtttga gaacatccat gacgagaatg aaggaacatc ctaaatgcgt
    16141 ttagttgaac caagtgccaa ataatatggc caaataagcg atcagttatc gatgaatccc
    16201 aagtattcta aactatatat tcattttgat gctaagcatt gatatatttt tttgggattg
    16261 atgaaggtta accaatacag ggactcactg atgaaattct agagtctgac cttggtatca
    16321 acgatttagt gattgtttct aatggaaaag tataaaagta tataagtaca ttaacaaaat
    16381 aactattagg gttacgctcc agcttgtgca aggcactggc aggaggaatc gtgaaatcat
    16441 aattatggtt aagccacacg gacaactaaa agtcatacaa agctatcgaa tcaatcaaca
    16501 gaagacaatc ggcaatcatt agctttgttt acagcttaca ttgcaacgct tgagttgaac
    16561 cgaaaatacg agggaggtaa cgcagccatt ttgagttggg gaatcttttt agaaaattat
    16621 cgggtacctt gttgtttagt caaaattata agggggagta atagtgagga acaaatttca
    16681 taaactgtta agtatgtttc tagttatgct tattgtgatc aatcaatttg ggaacataac
    16741 ggttctagct gagtctttag cagaacagcc agagcgttcg ctgacgatcg aagttgatca
    16801 agaggaggct gctttgggtg agcagttcca gttgcgagtc agaggaacac cagacctgct
    16861 aaatgcattg gtcattgata agagtgagga tttgattgaa ggggaagtga agcaactcaa
    16921 tgattacgaa cgtctgatta cttattcttc ccagcattca ggtaaatacg aattgaccgc
    16981 aagaagcggt gatattcaat caaatacggt ctcaatagaa atcagtgtac cagaagaaag
    17041 cacaagtgag tcttttaatg taccagaaga aagcacaagt gaatctttta atataccaga
    17101 agaaagctta agtgaatctt ttaatgaagc cagatcatct gagccatcta taacgcagga
    17161 atctcagatt gaaagacaag aggagctctt gaattatgac atcggcatct ctgctgagcg
    17221 ccccactttg ccgagcaggc atacacaaac gttaaaggga tacattcaag gaggcgctgg
    17281 cgatattctt gaagaatatg gggagatggt cgtacgagtt cctagagcat cgattggctt
    17341 aattgagttc attagagtgg aggtcgctga tgattcacct ttaaagccac tggcatttta
    17401 ccaagaaggc acagacgttg tttttccatt tacctttgat tatgaaggat taaatgaagc
    17461 cgctcagtcg tatacattca gtatcacgtt tgaagttgct ttctttcaat caggcggtac
    17521 tgagccaaat aatgactttc aagtgagtct tacttatgct gagcgagcag caaacacgga
    17581 attttcaatt acgcctattc cttcatcaag acccattttt tcgaaattat actatggctc
    17641 tagagaaatc gacagccaag gaaatcttgg gaatctgctg attgacgggc aagacgtgac
    17701 caacagtaat aaatttattt tgcaggtaaa ttatgaagga tcacgaaaag acggtgtagt
    17761 ggttaccgat gtactaccgc aaggaacaac tttggcaccg aatccgacta catggagttc
    17821 cactggaaat agagaaccta tttcaggtgg ggttcggatc gcagaagtaa cgaatccagg
    17881 tgaagcaaac gttagaactc attatgtttc cgaggaattt gtggacaaag tatcttttaa
    17941 taaggaaacg aatacacttg ttgtggactt tggtaatcta gcagagggtc aatcgtattt
    18001 tattgaatat gctcttgaag tgaccgatgt cgacttgttc aatgaaggaa gacaagagga
    18061 tgggttttct cggaatcgag cgagtatgac ctacaataat caggtaatga gccgagacta
    18121 ccgcttgaga atccctgaaa gtaatcttcc ggcaatctct ttaagaaaaa gtgtggacaa
    18181 aaatgttatc aatctcaatg aaaatgaact aacgtatact ctgcatgtct cgatgttttc
    18241 tggaacaaga aatacgcttg ttattgaaga tgttttggaa gaaggcatgg aatttgtgtc
    18301 atttctatca gatattccct ctttcattga tgccgaagtc aataatggaa tagttcgttt
    18361 cagtgcgaat aatacgattg gtggtccgac aagttttacc ctctctttta aggtcaatgt
    18421 agaggcgtat gcggtcggcg atcaaatttt aaactttgca acgcttgtga atagtgacaa
    18481 tcgattgaat acgagtactg taagaacaag gaaaatcgat gggcgtatta aagtaattaa
    18541 aactgatgag aatggccaaa ggctggcagg agcaaaattt gaaattttat caagcgattc
    18601 acaagtgata caagaaggta taacggcaga aaacggagaa tttttaagtg aaccgcttcc
    18661 tgtaggtagc tatcaagtcc gagaaacaga agcaccggaa gggtatcttt tagatgcgac
    18721 ggtacatgat gtttttataa atgaatactc catagaagaa gtgacgttaa ccattgaaaa
    18781 catccttgat acgggtggtg ttgaactaat taaacatgct gaaggcagtg gtgaagtcct
    18841 tcaaggagct gtttttgagt tacagaaccg agaaggagaa acgctacaaa ccggt
//
LOCUS       DAFEOM010000052        18370 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.053, whole genome shotgun sequence.
ACCESSION   DAFEOM010000052
VERSION     DAFEOM010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.053"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(25..972)
                     /locus_tag="LP524_001949"
     CDS             complement(25..972)
                     /locus_tag="LP524_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBL2234981.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            1366..2658
                     /locus_tag="LP524_001950"
     CDS             1366..2658
                     /locus_tag="LP524_001950"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBL2234982.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            2673..4442
                     /gene="aspS"
                     /locus_tag="LP524_001951"
     CDS             2673..4442
                     /gene="aspS"
                     /locus_tag="LP524_001951"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBL2234983.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            4551..5426
                     /locus_tag="LP524_001952"
     CDS             4551..5426
                     /locus_tag="LP524_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL2234984.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            5692..7575
                     /locus_tag="LP524_001953"
     CDS             5692..7575
                     /locus_tag="LP524_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBL2234985.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(7650..7934)
                     /locus_tag="LP524_001954"
     CDS             complement(7650..7934)
                     /locus_tag="LP524_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBL2234986.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(7924..8658)
                     /locus_tag="LP524_001955"
     CDS             complement(7924..8658)
                     /locus_tag="LP524_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBL2234987.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            8787..9410
                     /locus_tag="LP524_001956"
     CDS             8787..9410
                     /locus_tag="LP524_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBL2234988.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            9602..10723
                     /locus_tag="LP524_001957"
     CDS             9602..10723
                     /locus_tag="LP524_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBL2234989.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            10730..13858
                     /locus_tag="LP524_001958"
     CDS             10730..13858
                     /locus_tag="LP524_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HBL2234990.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            13877..17071
                     /locus_tag="LP524_001959"
     CDS             13877..17071
                     /locus_tag="LP524_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HBL2234991.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(17217..17924)
                     /locus_tag="LP524_001960"
     CDS             complement(17217..17924)
                     /locus_tag="LP524_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBL2234992.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         6142 a         3246 c         3869 g         5113 t
ORIGIN      
        1 ctataaattg ataaggtcaa ttgatcaaaa tccacgaaat gcttcatcaa tggtttgata
       61 atcttcattg gataaatgca aatctaaagc ttggacattg tcagatactt gctctggacg
      121 cttagctcca ggtataacta cagacactcg tggattcttc atgtaccaag ctaaaacaag
      181 ttgcgcagga gtggcttggt atcttttagc aatggggcga agtacatcga cagcggttaa
      241 aatttctctg aaacgttctc cttggaaatc tggatttctt tttcgaggat ctccttcacc
      301 aaattgtttt tcctcaccaa gttcatattt tcctgtcagc aaaccggaag caagaggaaa
      361 atagggaaca aaactgattt tgtttttttc aagataaggg aataattctt tttcagcctg
      421 ccgatggatc aaactgtatt tatcttctac tacatcgaca tatccatctg cattcgcttc
      481 tttcaattgt tctaacgtga agtttgacac accgactgct cggatttttc ctgcttcttt
      541 taattcgtgc aatgtagcaa ctgattcatt ttttggtgtg ctttcatccg gaaagtggat
      601 ataaaaaatg tcgatgtaat cagtttgcag acgttttagt gcatcttcca ccgattgttt
      661 gagaaactca ggagaattat caaatgttcg ccctttattt ggtacatgag cagcttttgt
      721 agcgatgacg acacgtgagc gatcatattc tttttccttc agtacctcgc caatcagttc
      781 ttcggaacgg ccgtttccgt aagcataagc ggtatcgatc aaattgatcc cgctattcaa
      841 agctgtacga actacttgtt ttcctgtctc atcttccaaa ccgtcaaaaa gattatgtcc
      901 tccaacggca tttgctccta atccaagctg ttctgcgtag acttgtgaat gtccgattct
      961 tatttctgcc atagctgatt cctccttttt gtctagtcta aaacaaaata ccaaaaagct
     1021 gaactaaaac gtttttgtga ggggaaatag tcagatatct cttgactttg agactggttt
     1081 ttagtaggtt ataagtaata tcgaatgtca atgaaagagc gagtaagttt ttgaaacttt
     1141 acaagagagg atcatcgagg ctgaaaatga tctaaagttt cattagccta aagacactct
     1201 ggagatggac gagtgaaaag ccgtcccgcg tacgagcgtt attcgtaaga ggaaagagaa
     1261 atctttcaaa attaggtggt accgtgtatg aatttcattc acccttgttc atgatgaaca
     1321 agggtgattt ttttatttac atagctaaga aagggagaga aaactatgag tttccaaaga
     1381 ccaaaaggaa caaatgatat tttgccaggc gaatcagaaa aatggcagtt tgtcgaagaa
     1441 acagcacgtt tattattcag agattatcag tataatgaaa tgcgtacacc gatttttgaa
     1501 cactatgaag tgatatcccg aagtgtaggg gatacaacgg atatcgtttc aaaagagatg
     1561 tatgattttt atgacaaagg ggaacgccat gtgacgcttc gaccagaagg tacagcacca
     1621 atcgttcgtt cttttgttga gcataaattg ttcggcccag agtatgcgaa accgttcaaa
     1681 actttttata tgggaccaat gtttcgctac gaacgccctc aaaaaggacg gttacgtcaa
     1741 ttccatcaaa tcggagtaga agcatttgga tcagaaaacc ctgcaactga tgtggagagc
     1801 atggtgatgg cacttgattt cttcaaacag ttggggatca cgcaaatccg gctagtcatc
     1861 aattcattag gtgataaaga aacgcgtcaa gcctatcgtc aagcattgat cgactatctt
     1921 ttaccttttg aatcagagct gagtgaagac tctaaacgcc gccttcatga aaatccatta
     1981 cgcgtattgg acagtaaaga caaacgagat caaaaatttg tggcagaagc tccatctatc
     2041 ttagattatc taagcgagcc tgctcaaaaa cattttgata ccgttgtttc catgctggat
     2101 gcattggctg ttccttatga aatcgacagt aatatggttc gcggactaga ttattacaca
     2161 catacgattt ttgaaatcat gagtgacgca ccaaaaatgg gggcacaagc aacgatttgc
     2221 gcaggcggac gttatgacaa tctagtggaa gaactaggtg gtccttcaac acctggcttc
     2281 ggatttgcaa tgggtatcga gcgattactc ttgactatgg aggcagagga agtagttatc
     2341 cctgcattta atgagttgga tgcttacgtg gtcgcactcg gagacgaaac aaatatcgaa
     2401 gcattgaaag tggtccaagc gattcgaaac tttggttttt cagctgaccg cgacttcatg
     2461 aaccgtaaag caaaagcgca atttaagaca gcagataaag cgaatgccaa attagtattg
     2521 gtgatcggtg aagatgagct tgctagtcaa acgattaatg tcaaagcaat ggcaagccga
     2581 aaagaaaaaa gttttccatt agaagagata tatgaacatt ttgatgaagt ctatgatgaa
     2641 atgactttat tcgagtagga ggaaaaaaat aaatggcaca aagaacgatc tattgcggat
     2701 tagtttccga acaacaatta gaacaaacag ttacattaaa aggatgggta caaaaaagac
     2761 gagaccttgg gggagtaatc tttatcgacc tacgtgatcg tgaaggaatc gttcaaatcg
     2821 tctttaatcc tgaaatcaac aaagaagcat gggaaatcgc ggataaatgt cgcagtgaat
     2881 acgtgatcga agtgactggg gaagttagaa gacgcggaga aaatgcagta aatcctaaaa
     2941 tggccactgg tgaattcgaa gtgattgcaa gtgaaatcgt gattttgaat caagcaaaaa
     3001 ccccgccatt tcaaattgaa gatgatcaag caatcagtga cgaaatacgt ttgaaatatc
     3061 gttatttgga tctgcgtcgt ccaaaaatga ctaaaaacat caaattaaga aatgcgacaa
     3121 caaaagcgat tcgtcattat ttagatgata atgactttct cgatatcgaa acaccttacc
     3181 tagggaaatc tacaccagaa ggcgcaagag actacctagt tccttcacgt gtccatgctg
     3241 gacatttcta tgcgttgccg caatctcctc aattgtttaa acaattgctg atgaatgctg
     3301 gattcgaccg ttattatcaa atcgttcgtt gtttccgcga tgaagattta cgtggagacc
     3361 gtcagcctga atttacgcaa gtagatattg aaacaacgtt cttaacagca gaagaaatcc
     3421 aaacttacac agagggattg attgcgaagg taatgaaaga agttcgcggt atcgaagtca
     3481 cattgccatt tcctcgtatg acctatgatg aagcaatggc tcgctacggc agcgacaaac
     3541 cggatacacg ttttgatatg gaattgatcg atctaagcga tacagtcaaa gaagtagaat
     3601 tcaaagtctt ccaaatggct cttgaaaacg gcggtgtcgt gaaagcattg aatgctaaag
     3661 gcgctgcaga tcgctactca cgaaaagaca tggatcagtt aggtcaatat gtaggacaat
     3721 tcggagctaa ggggcttgca tggctgaaag tagaagaaga cggattgaaa ggtccaattg
     3781 ctaaattcat gggagaagca acagaagcaa tcattaaagc aacagatgca aaaccaggtg
     3841 acttgctgat gtttggcgca gataaaagtg aaatcgtcgc tgctgcatta ggtgctatcc
     3901 ggacacgttt aggaaaagaa ttaggactaa ttgatgaatc gaaattcaat ttcttatggg
     3961 taactgattg gccacaattt gaattttcag aagaagaagg tcgatatgtc tctgctcacc
     4021 atccattcac gatgccaaaa gaagaagata tcccgttatt ggctgaagac cctgcaaaag
     4081 tctatgcaga agcttatgat atcgtcttga acggttatga actaggcgga ggttctatcc
     4141 gtattcatac tcgtgagctt caagaaaaag tactagaaac tctaggcttt acaaaagaac
     4201 aaatggaaga acaatttggt ttcttgttaa cagctttaga ttacggattc cctccacatg
     4261 gcggtattgc attaggattg gaccgttttg caatgttatt agccggagaa gataatattc
     4321 gtgaagtcat tgcctttcct aaaaacggaa aagcagcaga tgtgatgagt gatgcaccaa
     4381 gtatggtttc gcctttacaa ttgttcgaat tgaatattga tgtaacgaat atagaagaat
     4441 agccgataaa aaggtaaaaa ggatttgtcg aatacgcttc ttttgacaac cttgatttaa
     4501 ttgaaaaaca ctttataatg aagccataac atataaagcg gggcttttcc atgaaaaaaa
     4561 cattcgaaaa ttgggcttat catgattaca cgaccaagct ttcggtatct atcgtttatg
     4621 cgttgatcgc atcagtcgca ttgaatttct tttatcagcc aggacatatt tattcaagtg
     4681 gtattactgg tctagctcag attttgacga ctttgtcaaa atcactgata ggattcgaag
     4741 taccggtatc aacaactttg tatttactga atataccttt gtttgtattg gcgtggttaa
     4801 aaatcgggaa gaagtttaca attttcactt ttatcacagt cactttgacc tccctttgta
     4861 tccatatttt accggaaaca gtcctatcta ccgatccaat catctgtgcg ttgtttgggg
     4921 gggctgtgat gggactaggt attgggctag cgttgagaaa cggcctatca tcgggcggtc
     4981 tagatatcgt gagtatcacg attcgtaaaa aaacaggaaa gtcgattggt tctatttcga
     5041 tctacttcaa tgcattgatc gtttttgttg ccggaattct atttggctgg caatatatgt
     5101 tttacagtac gttgtctatt ttcgtcagtg gaaaagtaac tgatgtcgtt tatactaaac
     5161 aaaagaagat gcaggtaatg atcgtaacga aacatccaga aaaagtagta aatgaaatcc
     5221 aaaactgtat gcgtagaggg atcacgatca tcaatgaagc agaaggtgct tataagcatg
     5281 ataagcagac agtattgatg acgatcgtta cacgatacga actgcctcta cttagagaag
     5341 caatgaaagc ttctgatcca caagcttttg taagtattgc agaaaacgtc cagatacttg
     5401 gtcgtttcta tgaggatggc atttagatcg agtttgacaa aaattcatga aatactgctg
     5461 tagacagacg ttcgtttgtc tgcagttttt ttctaccata gtagaatcag caaagaaaaa
     5521 ctgctttttt atttgaaatt ttcttaaagt tgaaaaaaag catgtttatt tttttaaatt
     5581 tatgggatga tagaaaaaaa tagtagagaa aggacacgat tctacttgct attttcactt
     5641 aatacccgta tattacgaat gaaaaaagtt tatgaaaagt tggaatattg aatggaaaaa
     5701 caaaaaaggt taaaaaacag ccgctacatc actgggtttg atgggctccg ttcattagct
     5761 gtggtgggtg ttatcttcta tcaccttctg ccaactagta tgaaaggcgg ctatttgggc
     5821 gttccgattt ttttcgtcgt atctggttat ttgatcacgg atctgctgcg gcaggaatgg
     5881 gaacaaaatg gaaaaatcaa tatttggcaa ttttatgtca gaagaatgaa gagactctat
     5941 ccggcacttg tttttatgct tgtgacagct tcggcttata tcactttgtt tcagcgaggg
     6001 ctgttaaaca atcttcgagg agttgtggtc agcagcttac tgtatgtcaa caactggtgg
     6061 caaatcaata acggtttatc ttatttcgat cgatttgcta atgagtcacc gtttacgcat
     6121 atctggtctt tagcagtaga aggtcaaaac tatttggttt ggccgatctt gtttgtattg
     6181 ttgatggtat ttgttcgtaa aagaaaatgg atcttttata ctgtgttagg tacatccttc
     6241 ctctccgcca tcttgatgat gatcttgtat acaccaggag gcgatccaac ccgtgtttat
     6301 tacggtaccg acactcgtct gttttctata tggttgggaa gtgcgcttgc gtttgtttgg
     6361 ccaagtacgc gtttaaagaa aaacatcccg aaacaagcga aaaatattct gaatatcgct
     6421 ggaggaatct cgcttttggc actgattctt tgcttcttct ttttagatga tcattacaat
     6481 ttcatctatt acggaggaat gctcctagtg agtctgttat gcgtagtgct agttgcagtg
     6541 acagcacacc ccggagcaag tttgaatcga tggctgacaa atccagtatt tacttggatt
     6601 ggcaaacgaa gctatggcat ttatctgtat cagtttccag ttatgatttt ttatgaagca
     6661 aaggtgactg atattgccga tcatgttttg ttgcatactt tagtggaaat cacattgatt
     6721 ctaatcatca gtgaactttc ttatcgatat gtggagcgtc cgctagcacg ttttgattat
     6781 ggtcgtacat tccaagtgat gaaaggatgg ttcagcaaac cgatcatcag taagagcaaa
     6841 ccatgggttg ttccaggcac attacttgta gcaattgcac tcgttggttt cgttacggca
     6901 ccgaaaaacg ctgtaacagc cgatcaaaaa caactgcagg caaaaatcgc tgaaagtaaa
     6961 aaattagcag aagaaaccag aaacaattca gaaaacacca ctagttcggt tgaccaagcg
     7021 atccttgata agtacgagtt gtccaaggca caagggaata aggcacaaaa attagaacta
     7081 acggcatttg gtgattcagt tatgttggat gctgcaacgg atctacaaga actttatcca
     7141 aaagtagtgg tagatggaga tgtcggacga caattatatg ccagtgtacc gtacattgaa
     7201 aaattaaagg aacaagattt gttgaaagac actgttctga tcggactagg aacgaatggc
     7261 tcattctcgg aagctcagtt tgatagtgtg atggatgcaa tcggtgatcg aaaagtatac
     7321 tgggtcaatg tccgagtacc gacgaagcgc tggcaaaatg acgtgaatgc gatgcttgaa
     7381 cagatgacga aaaaatacga caatatgacg ctgattgatt ggtacagcta cagtaatgac
     7441 catagtgact ggttttatga tgatcaagtc catccaaatc ctgaagggat gacacattac
     7501 attcacttag tcagcgaagc cttgctagga ggaagttcaa gtacgaacag cagtgagaca
     7561 aacgtgacag aatgatagct acaaaaaact tcaggcaaaa acatttgttt ttgcctgaag
     7621 tttttttata tttaccgtgt ttttaacatt catatagatt caggaagcaa aacggttgag
     7681 tgtgtctgta aataacgttc tttttgtttt cgggtgagtt ttttgaatgc agcttctgct
     7741 tttgttgctt cgcttcttga agaaaatact tctgcatgga tcatcttaac aggtcttctt
     7801 ttttgaagac gagtatattt agcaccagta ccattgttat gttcctctaa tcgtctagaa
     7861 agatcggttg tatatccacc ataaaatgtc tgatctttac agaataatac gtaaaaataa
     7921 tgttcatttg ccatagagca tctcacgaat ctctgcagtg tattctccat ccatattgta
     7981 agtgatcaat ggtggagcga ctttgaatcc gtctaatttg ccttctttga ttccttcaac
     8041 taacaaaata tttgcctctt ttccaggttt tgggtaaaca aattggatcc ttttaggtgc
     8101 aattcgatag tcttccatga cctgaatgat ttctaagaac cgatcaggac gatggaccat
     8161 agtaaagcgt ccatttgttt ttagtagctt acttgaaata tcgatcactt cttctaacgt
     8221 cgtatgaatc tcatgacggg cgattgccag gtgtttatta gggttcgttt tatttgttgg
     8281 cagccctttg aaataaggtg gattacagac gactaagtca caagaattat gtcgaacgac
     8341 agataagctg tctttcagat cgatggtgtg gacagtcatt tgttcattta aatggttaag
     8401 cgcgatacta cgtcttgcca tgtctgctag acgaggttga agttcgatag cgtcgatagc
     8461 cgcctgggtt cgtttactta aaaaaagtcc aactgcgcca tttcctgcac aaaggtcaac
     8521 aatcttccct tttttaggta cacgggaaaa gtgagcaagc agcacagcat ctaaagaaaa
     8581 agaaaacact ttggaacttt ggataatctt gatatcttct gcgtataatt ggtcgattcg
     8641 ttcgtcttcg tgaagcatgg attccctcca aaatgaaaaa aatagtcgtc tgtattatac
     8701 tatttttatt aaaaagaacc aaggtggctt gtcaaacctc tattttttat gcatactatg
     8761 accataagaa gggaaaggaa gagactatgt tttatcgatt tatgcgcggg gtagttcgag
     8821 tactattagc cttagttaat ggaaatgccc gctatgagaa taaagaagtt ttaccgcaag
     8881 atgagaatta catactagtt gctccccatc gtacttggtg ggacccatta tatctggcgg
     8941 tagctgcagc acccaaggaa ttcagcttta tggcaaaaga agaactgttc aaaaatccag
     9001 ttctgcgatt tatattaact cgatccaatg cttttccagt taaaagagaa aatccaggac
     9061 caagcgtcat taaaacacct gtaaagattt tgaaatcgac aaatttgagt ttgatcatgt
     9121 ttcccagtgg gacaagacat gctacagaac ttaaaggcgg aatggcattg atttccagaa
     9181 tgtctaaagc gaaaatcgtg ccagctgtat atcaaggtcc gttgacactt ggggatctgt
     9241 tcaagcgtca gagagtgaca gtgcgtttcg gcgaaccaat cgacttatca gatatcaaga
     9301 aaacggataa agaaggtatg gcggagatcg aacgacgaac acaagcagct tttgacaaat
     9361 tggacaaaga agtgaaccct gatttcaaat atgaagtcaa aaaaaaataa aatgataagt
     9421 ttatgcgtgg gcggtctttt gaatctactg tcctgttcag ttcagacttt ttcaatgaaa
     9481 acgagggaga gacgtcaagt ctgccctctt tttgcgttcc aatatgtaaa atagaagaca
     9541 ggtgtcatgt aatggatcaa agtgttataa taattagaag gacatttttt gaggtgattg
     9601 aatgcgtttt ttacatacag ccgattggca tatcggaaaa aaactacaag gttatgactt
     9661 gattgaagac cagcgtcatg tactatcttc tatactagat atcgcgaaag aagaaaaagt
     9721 agaggcaatc gtgattgccg gagacttata tgataggagt gtcccagctg ttgatgccgt
     9781 agaattatac aatgaattga tggtcgattg gaatttaaag gaaaagatcc cgatttttgc
     9841 tatatcagga aatcacgata gcagtacgag actttcagcg ggtacgccat ggttcagtta
     9901 ttcgaactat tttttgcgaa cacagctagc agaagcattt gaaccagtcg agtttaaaaa
     9961 cgtacaattt ttctttcttc cttactttga accaattgca gcgagattat attttgaaga
    10021 tgacaatatc cgaacgatca agcaagcggt agccaaggtg atagataaaa tgcaggagct
    10081 atttgattca gaaaaaaaac aagtattgat tagccatttt tttgtaatgg gaagtgtaaa
    10141 atctgattcg gaaacgaaaa tcgaagtagg cgggttggat tcgatccctg gtgaactact
    10201 tacaccgttt gattatgtag cactaggaca tttgcataat caagctgctt tgagacagga
    10261 taatgctcgc tatagcggtt ccccattgaa attctcttta tctgagatca accaaaaaaa
    10321 aggcgtatgg atcgttgacc tgacaacagg cctggagttg aattttaggg agatcgaacc
    10381 cctgcatgat attgaagaaa taacagcgag tttcaaagaa ttgctggatc ctgcctttta
    10441 tcagtccgtc aatcgggaga attacatcta tatctatctc actgatagaa cgatcatccc
    10501 gaatatgatg aaccagcttc ggcaaatata tcctcgtatt ttggggattg aacgggcaaa
    10561 cggtcgagca gaacaaacaa agcgcaaacg aaaagtacta gaaatgaaag atcctcaaaa
    10621 attagcagaa gcatttttta gtgaaatgac ggaagagcag ctaactgcaa accaagaaaa
    10681 atggctcgga gaaacgttgc aagaaatcat ggaaatggaa tgatcaagaa tgaaaccgaa
    10741 aaaattacaa cttaaaaatt ttggtccttt tatcaatgag accgtagatt tttcacgttt
    10801 gacagaagca ccgctttttt tgatcagcgg gaagacagga gctggcaaaa cgacgatttt
    10861 tgatgggatc accttcgcac tttttggtga aacttccgga agacttcgct ctggaaaaga
    10921 gatgcgttct ttgtttgcta caccagacga agaaacaagc gtgacttttt cttttgaaca
    10981 tcagcagatg agctatgaaa tcgaacgaaa accagaacaa gttttagcaa aacgaaaagg
    11041 aaacggaaca aaaaaacagg cagccaaagt aacgttgacc atttttgatg ggaaaggaaa
    11101 agaaataaaa caaatcacaa aacgtacaga agcagatcag ttgatcagag aactgatgaa
    11161 tctcgatgcg aaacagtttt ctcaaatcgt tttactaccc caaggagaat tccgtaattt
    11221 tttggtttca tcaagcagtg ataaagagac tgttttacgt catttattcg gtactcaatt
    11281 tttccaacag ttcaatgagc gattaaaaga aaaagcaaaa caacagcaac aaaaattgga
    11341 tcatttagag caagagctgc atcttctgca aaaaaggttt gttttagtca aagaagagga
    11401 accagcagca gggtttgaag ccgttttagc ccaatggaca aaccatcagg caatactcga
    11461 aaaacaaatc acggaagaaa aagaagctct aacggaactt caggaaaaac aaaagcaact
    11521 agaaaattcc tactatcgtt ttgaaagctt gaaaaaaagt tatgaagaaa aaacgatatt
    11581 aataaacaag caaaaggaat tagctgctca aaaagacgaa atagaggaaa agaaacgatg
    11641 gattgggtat tatgaattta cgcaacgatt agtcgaacca atccagcgcc tacaagaaat
    11701 aaaagttgaa aaaatagaac tgtcacaaaa aagtagtgag caaacaaaga aactagcgat
    11761 gactacgcag aattatgaag agtggcaaaa aaatgaagca aaccgcgtac gccgacaaac
    11821 aaaaatcaaa gaagctgctc aaaaactgca aaacaaacag atgatggtgc caattgtcga
    11881 atccttacgt agtaaaaaac aagacgcaga acaattaaca atttcattgg ataaagaaga
    11941 attgaatgcc gagaagctgt atcaaaatct cgctatctat caggaaagaa ctcgaattct
    12001 tcaagaaaat ttactggatc aggaaaaact gcaagaagat cgtcttgctt ttggacagtt
    12061 aaaatatcta gatgaaaagc agcaagaaat agaaaaacaa aaagcaaaag acactgggtc
    12121 gctcgcacag ataagagaag aactcaaaaa agcaactgaa gaaataacaa aaaatagtca
    12181 gctattgaaa gaacagacag aacaagcaga acagctgaaa agccaatggg caaagcagca
    12241 aattgctcgt cttcagcttt ttttgcgtcc aggggagcca tgtcttgttt gtggatcaac
    12301 ggaacacccc atacaacaat taaaacacga acagccaaat aaggaagata tcttaaaagt
    12361 agaacaacaa ctggcagaag ctgagtctgc tgtagaaaga actcagcaaa tcattgccaa
    12421 aagtgaagca catgaaaata atcttcaaca acaattatca gatttagaac aaaaaatcat
    12481 ggcaacgaat atacaattgg ctgaacaaga agaacacttg caacaggctt tgaaaaaggc
    12541 atttcctgag ataacagaac aaaaaacaaa aacaactctt gtagtaatcg aacaatcttt
    12601 agatagacga aaaagcaaga tggccaatgc aagaatagag ctggaagaaa taagacaaca
    12661 agcagaaaag ctcgaacaac aattagctgt ctacaaacag cagcagacag aaaaaaataa
    12721 tcagctccag caactgcaag gagaaatcaa aagtttagaa caacaattgc aagatacgga
    12781 tagtaactgt ttagaagagg agatacgacg acttgaaaaa cagttagaag aagactcgaa
    12841 tcaactagct gttgagcaga aagcaggagt gcaattgttg caagatcttc atacgcttcg
    12901 gacacagctt gatcttcaac atgaacaaat agaaaaaata gatgaaaaaa tagaaaagga
    12961 acaagaaaga atagctaaaa agttagaaag ccagtcgtat ttttcaacaa ttgaagaaat
    13021 ctgtgcattt gcacaacagc aagcacaata taaagaaaac caacgtacag ttacttctta
    13081 tcatgaagag caattagtga cagctgaccg gctgcgtcaa ttagaaaata tccagctaac
    13141 tgagacattc ccagatacag aagagatcag ccaacagtta gaagaaataa gacgtatgat
    13201 cgaaataaag caagaaaaga tatacggttt acaagaacaa aaaattagta atcaaaaaat
    13261 ctacgatgaa tgtctttcga tttatcacac aagccagacg aaattagatg agcttacgca
    13321 attacagcag ctttcgcaaa cattcaacgg agaaaatcct aagaagacta gtttggaacg
    13381 gtatgtgctt caggtttatc ttcaagaagt gttacaagta gcaaatgatc atctccaacg
    13441 tttaacgaaa aatcgttatc aatttgagct agcggatacc attggcagtt atcgagggaa
    13501 aaccggatta gaaatcaata tctatgatga tcaggcaggt atgtctcgaa gtgcgcatac
    13561 cctgtctggc ggtgagagtt ttattgcagc actttcttta gcattagctc ttgccgatgt
    13621 gatccagaca caagctggtg gtgtagcaat cgatgcattg tttattgatg aaggatttgg
    13681 gtcattagat gaagaagctt tggaaatggc aatggaagct ttagaaacga tcgaaagtga
    13741 aggaagaatg atcggcatca ttagtcatgt aagagaattg aaagaacgag tattacaaca
    13801 aataagaatt gatacagaag gtagcggaca aagcaaaata cgctatcaac ttggataaaa
    13861 agggagattg gtaactatga aatggagtat tccagaaaaa gtcattgcac gtggacgtca
    13921 atacttgcaa gaaggccgag tgttatcggt tgtccctgac caagaagaaa acgtgtggca
    13981 tgcagaagtc ttgggcagtg aattgtatat ggttgatttg gatggtacag caaaagagat
    14041 agattactgc caatgtccct attgggagga acataaatat tgtaagcata cagttgctgt
    14101 ggaactttat ttacggtcga aaaatctatc tcgtatcatg aaaaaagacc agccaatgct
    14161 agaaccggta aaagcaacaa gtgagggaga aatcattgca aaaggttttt ctcgtctaaa
    14221 aacacaaaac aatcctcaag cggtgcaacc tttgattatt gaataccaag tagaaacgat
    14281 tgagactaat cagtatcacc cagagctttc tattttggca ctttacttgc gtatcggttc
    14341 attggcatca ccgaaaaaaa catatatcgt aaaaaacatc tatgattttt tacaagctta
    14401 cacagaaaaa gaaacttaca ctgttaataa acagtaccag tttcgtctag ataaacgggc
    14461 gtttcaaaaa gaagatcaaa aaatactcac gcatcttgca ggaagtgctc agacgcatca
    14521 attattaggt acgaacggct tacaagtcaa aggaaaacta gataaaagat atctgcttct
    14581 cccagtagaa caagcccgct tcctgttaga gcagatgaat cagactacac gtttgcatct
    14641 gcagataaat gaacaaaacg atcaaggaat acaattttcc gatcaaaaca aacctttatc
    14701 attcaaagtg gaaaaatttg gagaagatta tttgctgcaa gcattgaatg attttgattt
    14761 tttctttatg cattatcagt ggggagttat ccaagggaca ttttattctc tgacgaaata
    14821 tcaacaaacg gtttatcaaa cttggaaaca attgattcgc cgattggaga aaccagaagt
    14881 agtgttcaag aaaaaagaat taccgtcttt gtttaaagag attatccctc tgctgtcaga
    14941 gatcggcgac gtagctattg cccctgcagt agaagaggaa gtagcagata tccccgtcga
    15001 atttattttc tttttccgaa aagcaaaagg aaaaatccaa gctcgagtcg atttcgtcta
    15061 tggcgaagtc atctattcga ctgacccgaa gcatgaggtc tcgacagaac aggcttttca
    15121 ggtgttacgt gacacagtag aagagaaacg agtacttgat ctattcagaa tgtatcgcta
    15181 ccagaaaaat gaaactggtt atgaacgtcc attgccaagc ggggaagaac tatatgcctt
    15241 tttccgcaca gaactatctg tcttccgtca gtttggtgaa gtcagacttg gaagaaaatt
    15301 gcgagagttg tatttagatg cgcataaaca tcaaccgaaa atcgaagtaa ccgagaccgg
    15361 ttcatggctc gatattcgat tcgatgtgac agggatagag gagcaagaaa tcgatcatgt
    15421 cctgcaaagc ttgctgagaa atgatgcttt ttatacatta gaaaatggtc aggttctttc
    15481 ttttgattca gaagaatttc aacaaacaag ccaagtgttg caacaattac gggaatcgat
    15541 acgtacagaa gaaggaacga tccatgtacc taaaaatcaa ggattgatca ttcaaaatca
    15601 attggaaaaa agcaatgcta cttttagtga atcgttccaa acgatggtac aagatctcat
    15661 tcatccagaa agataccagg cacagcttcc caaaggattg aatgcaacga tgagagatta
    15721 tcaaaagcaa ggttttcgat ggttgaagat gttaggtcat tatcagtttg gcggtatttt
    15781 agccgatgaa atgggacttg ggaaaacact acagacaatt gcctttttac tttcggaaaa
    15841 agaagaacgg aaaagcttct ctgctttgat cgtagcgcct gcgagcttaa tttacaactg
    15901 gcaggcagag gtgagaaaat tcgccccttc tttaagtatc caagtaatca atgggaataa
    15961 aaaagaacgt gaagaattac tggctaaaga cacagatatc cgagtgactt cttatgccag
    16021 cttaagacag gatctagcta attatcagtc gcaaaaaatt gattatttga ttcttgatga
    16081 ggcacaaatg gtcaaaaata gcagtacgaa aactgcacaa gcattgagag aattagctgt
    16141 acctcagcgg tttgcactga gtgggacacc aatcgagaat aatttagaag aattgtggtc
    16201 attgtttgca acgattatgc caggattttt cccgactaaa acaaaattca gggaacttgc
    16261 cgcagaagaa attgcgcaaa tgattcgacc gtttatttta cggcgagata aacaaactgt
    16321 gttgaaagac ttgccggaaa aaacagagat gaatctatat tctgcactta cagaagaaca
    16381 aaaaactgtt tatttagctt acttgcgtca aatgcgtgaa gaaatcacat caatggattc
    16441 tgaagcattc aagaaaaacc ggatcgggat acttgctggt ctgacaagac tgcgacaaat
    16501 ttgctgcgat cctcgtctgt ttattgaaga ttaccaaggc ggttccggca agctcgaaca
    16561 agtaaaagat ctactggtag ctgccaaaga gaacaaacga agagttttgc tgttttctca
    16621 atttacaggg atgctgacta tcttgcaaga agagttagcc gagctaggga tctcgacatt
    16681 ttatttaaga ggcagtacga aaccacaaga tcggttaagt atggtagacg catttaatgc
    16741 tggcgaaaaa gatgtttttc tgatttcctt aaaggctggg ggaactggat tgaatctcac
    16801 gggagcagat acggtcatct tgtatgacct ttggtggaat cctgccattg aagaacaagc
    16861 tgctggacgt gcacatcgga tcggacaaaa aaatgtggta gaagtgtggc gtatgatctc
    16921 tgaaggaacg attgaggaac gcatggattc actccagcaa gaaaaacgcg aactgttcca
    16981 aaaagtgatc caaggtaatg aagaacagtt gacgaaactg actgaagaag acatccgcat
    17041 gattttaagt gtaggagaaa tcgaggagta agagactcga aaaagcgttc agtaatgggt
    17101 aagaaagcct ttctaaagaa gcgattgttt atttgattta aaaaatagca aggaggctgg
    17161 gattccattt tatcccagct tccttgctat cattttttta tttctatttt taaaggttat
    17221 acaaagattg tcttcattgt ctctaacagc tgatcaaaag aagaaatctc gatatcgcaa
    17281 attggagtct gttcaatttt gcgttctcga tggttgaacc aaagcgcttg ccagttagca
    17341 ctttttgcac ctagaacatc attgtcgtaa ttatcgccaa cgtataaggt ctcttctgga
    17401 agcatatgaa attctttctc agctagctga aaaatctctt tttctggttt ttggaaacct
    17461 gttgcttgag aaatgagcat attgcgggaa ggaatccaat tgttcaattg aagttgcttc
    17521 agtttttttg tctgatggtc agtcggccca tttgtgataa ttcctaatgg gacattcttt
    17581 tccttcaaga aatcgagggt ttttttgacc tcctcatgca agcagatatt atcaagttct
    17641 tcctcgtaaa ttttttgaaa taagaggcca tcttcttccg taatatgagg gtagtctaaa
    17701 tcttttagtg attggctgat ccgatgtgct cgcatatatt ctagtgtcca atctcctgcc
    17761 attacttttg ggaagttttc atcactatga tggcggaacc ggatatatag tggatgcata
    17821 tctgcatctg aaacaagcgg aacgactctt ttgaccgcgt ttcgaaatgg ctgctgctga
    17881 tcatacatcg tatcatcaac atcaaacaca actgatttaa tcaaaatttt acacgccttt
    17941 cggttttcat tgattttttc acaaatcctt gactattgta acagaaaaaa ttgtctaaaa
    18001 aagctgataa ataaaggttt ttcttactat atttctattt ccttgacaga ataaggtttt
    18061 tatatttcag tttttttcac taaaaatcgt gaaacacgtc aataagtttg atgaacaaat
    18121 taatcattat ggtagaagtc gattgattta ttttaatgca atctctgtgg aattttcgaa
    18181 agatttattt ttttaataca gtctcaatat aactattcaa acataaaata acgttatttg
    18241 atttatttgt ttatagtaaa ttttatttag taatcattgc tttaaaatac tacgcttata
    18301 taacagagaa aataagcgtt ataataaaga acagtattat ttaaaatgac agaaaacatt
    18361 actattaaag
//
LOCUS       DAFEOM010000053        18272 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.054, whole genome shotgun sequence.
ACCESSION   DAFEOM010000053
VERSION     DAFEOM010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.054"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            <1..174
                     /locus_tag="LP524_001961"
     CDS             <1..174
                     /locus_tag="LP524_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234993.1"
                     /translation="SGTAVDDIEDVDQLKQLNALLTKQLAISVQEQEKMQQMLAQLTM
                     EVASIKNGGKSNE"
     gene            167..307
                     /locus_tag="LP524_001962"
     CDS             167..307
                     /locus_tag="LP524_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBL2234994.1"
                     /translation="MNSFPGFENIKQFYDWGCYTDQDLLDYVNMNCLTKDQYKQITGN
                     EI"
     gene            377..796
                     /locus_tag="LP524_001963"
     CDS             377..796
                     /locus_tag="LP524_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBL2234995.1"
                     /translation="MEKYLNTLSVMTGVIGGTIVGLLGGMDNILHVLIFLVGVDFLTG
                     LAKAWKLKEISSEIGFEGLLKKVLIFVVIAVAVDVQKIVGNSIPLREIVIMFYVANEG
                     ISFLENISVFLPLPDKLKEVFQQIRNDTENKDRGGTK"
     gene            793..1803
                     /locus_tag="LP524_001964"
     CDS             793..1803
                     /locus_tag="LP524_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBL2234996.1"
                     /translation="MKKKIFVGAIIALFLLPINAFAYSINNEFNLGANEGSSQVANNQ
                     YILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSYAN
                     ANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLWVT
                     QNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTL
                     SDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPD
                     VNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIP
                     KR"
     gene            1918..3126
                     /locus_tag="LP524_001965"
     CDS             1918..3126
                     /locus_tag="LP524_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234997.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEILKDILNSISTMEGVSEAISEEIQSYVYEIIIRDDVYSSPTGIVI
                     AGYGRMDIFPKLYSYNMFGFVMNILKYSEYESAQIGNDNGSLRSTILPFAQSDVVNTV
                     VQGVDPQITNYLSSQVDSFDDNGKNTYINIIKNISEFQQNQFIFPLLNMIALLPVEET
                     AIIAETLLNLTSFKRKYTTSVETVGGPIDVLAITPNDGPIWIKRKHYFDIDNNIGYRL
                     RKERANDYNN"
     gene            3110..3280
                     /locus_tag="LP524_001966"
     CDS             3110..3280
                     /locus_tag="LP524_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234998.1"
                     /translation="MITIIKPQEKSPVLKFNESNLAHNKMSITDTVFDVMVEQQKKIN
                     NNNRQRKEINQY"
     gene            3535..3608
                     /locus_tag="LP524_001967"
     tRNA            3535..3608
                     /locus_tag="LP524_001967"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3569..3571,aa:Ile,seq:cat)
     gene            complement(4121..4423)
                     /locus_tag="LP524_001968"
     CDS             complement(4121..4423)
                     /locus_tag="LP524_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2234999.1"
                     /translation="MLIEGKTQLWFKFDPSNRFIKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            4402..4809
                     /locus_tag="LP524_001969"
     CDS             4402..4809
                     /locus_tag="LP524_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235000.1"
                     /translation="MSFLLLKYLEIYSVKKNKLIGENDSPIKTPIELSLRNRMGILQR
                     SEIVKELFYHKFSYEIRLDDTYEHYRIVFFVYNSIEDATATTALTFGFTKNGTINSDK
                     TRQAATESDDICKKVCNGEENYWIGEEKLNEIY"
     gene            4796..5248
                     /locus_tag="LP524_001970"
     CDS             4796..5248
                     /locus_tag="LP524_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235001.1"
                     /translation="MKSINDLLKKHPDLYKKLNSEQRNLSKQLLKIRLKKQKNVTEFS
                     KMLGISEKKYLEFEFGDMHIPVAEYNKLLKQVENLSYKDSMNFTIKVAGGPIKYDRIE
                     NKPYIRNKSYSVFKSRKRKEVSSNMVYASVNYQDTSRYIVEKEVYVHG"
     gene            5241..5639
                     /locus_tag="LP524_001971"
     CDS             5241..5639
                     /locus_tag="LP524_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235002.1"
                     /translation="MDKGNSVTFALENISLNKIHFELTDNFSYGDLEFNIELGYKEDE
                     AEYLEKKLVSLRAHIVGGMEGAPVDILLFSVIRVSTNEQPYKKLVEESMGELAKPILN
                     KASNLISNLYSEVGVMPTVIDLYQLYSQQK"
     gene            5951..6024
                     /locus_tag="LP524_001972"
     tRNA            5951..6024
                     /locus_tag="LP524_001972"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5985..5987,aa:Ile,seq:cat)
     gene            6404..6565
                     /locus_tag="LP524_001973"
                     /pseudo
     CDS             6404..6565
                     /locus_tag="LP524_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            6715..6915
                     /locus_tag="LP524_001974"
     CDS             6715..6915
                     /locus_tag="LP524_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL2235003.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7169..8848)
                     /locus_tag="LP524_001975"
     CDS             complement(7169..8848)
                     /locus_tag="LP524_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBL2235004.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(8850..9062)
                     /locus_tag="LP524_001976"
     CDS             complement(8850..9062)
                     /locus_tag="LP524_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HBL2235005.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9455..11185
                     /gene="efrA"
                     /locus_tag="LP524_001977"
     CDS             9455..11185
                     /gene="efrA"
                     /locus_tag="LP524_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HBL2235006.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11182..12942
                     /gene="efrB"
                     /locus_tag="LP524_001978"
     CDS             11182..12942
                     /gene="efrB"
                     /locus_tag="LP524_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HBL2235007.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13037..13234
                     /locus_tag="LP524_001979"
     CDS             13037..13234
                     /locus_tag="LP524_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235008.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13387..13872
                     /locus_tag="LP524_001980"
     CDS             13387..13872
                     /locus_tag="LP524_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBL2235009.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(13976..14569)
                     /gene="clpP"
                     /locus_tag="LP524_001981"
     CDS             complement(13976..14569)
                     /gene="clpP"
                     /locus_tag="LP524_001981"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBL2235010.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            14749..15276
                     /locus_tag="LP524_001982"
     CDS             14749..15276
                     /locus_tag="LP524_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235011.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15367..16104
                     /locus_tag="LP524_001983"
     CDS             15367..16104
                     /locus_tag="LP524_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBL2235012.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16108..17025
                     /gene="miaA"
                     /locus_tag="LP524_001984"
     CDS             16108..17025
                     /gene="miaA"
                     /locus_tag="LP524_001984"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBL2235013.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17027..18256
                     /gene="hflX"
                     /locus_tag="LP524_001985"
     CDS             17027..18256
                     /gene="hflX"
                     /locus_tag="LP524_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBL2235014.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         6104 a         2985 c         3730 g         5453 t
ORIGIN      
        1 agtggcacag cggttgacga tattgaagat gtcgatcagt tgaagcaatt aaatgctcta
       61 ctgacaaaac aattggcgat atcggtacag gaacaagaaa aaatgcaaca aatgttagct
      121 caattgacga tggaagtcgc aagtattaag aatggaggga aaagtaatga atagttttcc
      181 gggctttgaa aatataaaac aattttatga ttggggatgt tatacggacc aagatttact
      241 tgactatgta aacatgaact gtttaacaaa agaccaatat aagcagatta ctggaaatga
      301 aatttaatta agcacaaagt taaataaaaa gcgtactcaa acgagtgcgc ttctttgcat
      361 aaaggagaaa tagatattgg aaaaatattt aaatacactc tcagtaatga caggggtgat
      421 aggcgggaca attgttggtt tattgggagg gatggacaat atactacatg ttttaatttt
      481 tcttgtgggt gtggacttct taactggact tgctaaggca tggaaattga aagaaataag
      541 tagtgagata ggtttcgaag gattgttgaa gaaagtctta atcttcgttg tgattgccgt
      601 tgcggttgac gtacagaaaa tcgttgggaa ttctatacct ctaagagaaa tagttattat
      661 gttctatgtt gcaaatgaag ggattagttt tttagaaaac atttcggtat tccttccttt
      721 gccagataag ttaaaagagg tattccaaca aatacgaaat gatactgaaa ataaagaccg
      781 aggaggaacc aaatgaaaaa gaaaatcttt gtaggagcta ttatagctct ttttttattg
      841 ccaataaacg cctttgctta ctcgatcaac aatgaattca atttgggcgc aaacgaaggt
      901 agttctcaag tagcaaataa ccaatacatc ctattgcatg aaacagcaaa cgaaactgcg
      961 acaggacgaa atgaagcgca gtacatgaaa cgttcttggt acaatgccta tacagcgtat
     1021 attgttggcg atggtgggat cgtttaccaa gttggacaac ctggttatgt acaatatggc
     1081 gctggttcat atgctaatgc aaatagtcct gtgcaaatcg aactgcaaca tacgcacgat
     1141 aaagtaacgt ttgaaaagaa ctacaaggca tatgttgaat tagcgagaga ttccgctaat
     1201 aaatacggta ttccacttac attagacaca ccgtataatc aaccaggaat caaatcacat
     1261 ttatgggtaa ctcaaaacat ttggggcgat cacactgatc cgtatggata cttatcagaa
     1321 atgggtgtaa gtaaggaaaa actggcttac gatttagctc atggatttac agacgaaaac
     1381 ccaacgactt ctgagaacaa gcctgtcatt gatccaaccc gagcaggtgc agcaaatcca
     1441 actttatcag atggaacgaa ccattctcac attgatcagt ttggagaaat cgaaaatgcg
     1501 aacttacacg tcgctggatg gcacatcgct aactataaat acgagtatat tttcattatg
     1561 gactacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagaccggat
     1621 gtaaatcaag cttataatac ttctggaaac gttggctatc atgtatcttt taacatgcgc
     1681 aatttcccta ataagaaagt atacgtcatg atgcgggcaa cgaatgatcc agaaggaaac
     1741 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
     1801 taaaaatagc tcctcgttga ggagcagtac atagaattga aaattaacgt taaatcaaaa
     1861 aaatatttac aagatgttta tttaagtaga taataaaaga aaaaagcagg tgaaattgtg
     1921 acagcagaaa ttggtattat gaataaaagt ggaatcgtgt tagcttcaga ttcagcatct
     1981 acgattggag atagcaaagt atataatact gcaaaaaaat tatttacttt ggattccatg
     2041 cattctgtag gtatcatgat atatggtaat gctgaattta atggtattcc ttgggaaata
     2101 atcattactc aatataagaa aagcattggt agttctgttt ttaatacttt ggaagaatac
     2161 gcggataatt ttatagaatt tgtcaaaaca gcatctttta ttagaagtga acagaccgag
     2221 caagaacaaa tgattggggt ttttcaaaaa ataatttcag gtttatttga gagtatagaa
     2281 gtagatatta actttttaat aagccaaggt acacaaatag acaaagatgt cctagtgaag
     2341 ttattacaaa caaaaatgaa taccaatttg tctcaacagt cccagacatt cattttagat
     2401 attgaaaagg cgttatttct tagtaactat ggagaaatat taaaagatat tcttaatagt
     2461 atttctacaa tggaaggtgt ttcagaagct atatcagagg aaattcaaag ttacgtttat
     2521 gaaattataa ttagagacga tgtttattct tcccctacag gaatagttat agcaggatat
     2581 ggaagaatgg atattttccc taaactatat tcttataata tgtttggatt tgttatgaat
     2641 attctgaaat actcagaata tgaaagtgct caaataggaa atgacaatgg ttcattaaga
     2701 tctactatat taccatttgc tcagtctgat gtggtaaata ctgtagttca aggggtagat
     2761 ccacaaatta ctaattatct gtcgagtcaa gtcgatagtt ttgacgataa cggaaaaaat
     2821 acctatatta atattataaa gaatatttca gagtttcaac aaaatcaatt catatttccg
     2881 ttactaaaca tgattgcttt actcccagtt gaagaaacag caattatcgc agagacttta
     2941 ttaaatttaa ccagttttaa acgaaaatat actacttctg tagaaacagt aggtggccca
     3001 attgatgttt tagctattac acctaatgat ggccctattt ggattaaaag aaaacattat
     3061 tttgatattg ataataatat tggatacaga ttgcgaaagg agagagcgaa tgattacaat
     3121 aattaaacct caggaaaaat cccctgtttt gaaatttaat gagagcaatc tagcccataa
     3181 taaaatgagt ataactgata cagtatttga tgtaatggtt gagcaacaaa aaaaaattaa
     3241 taataacaat agacaaagaa aagaaattaa ccaatattaa tgttttttct gttgcgtttt
     3301 ttgcagtcat aatttaaaac ctcgctcaat cttgggcgag gtcttttttt atttttctga
     3361 aaagttaaag taaataatag cataaaataa tatattttac aaagaataag tacaatctag
     3421 ttttttgcta ttaaatgtgt aataataaat atgccatcac aacatgaaga atgaaatcca
     3481 ttattatcta gtctagtcca ttctttttgt ttgcagtatt tgtgatggct ttccgtaccc
     3541 ttagctcagt tggtcagagc agacggctca taaccgtccg gtcgtaggtt cgagtcctac
     3601 agggtacatt aacgtagcca tttgaatcgt tctgtgttag aattttttga agagtattat
     3661 acaagctaaa gcttttcttc attgccactc aaatgagtgg cttttttatg tatcctttta
     3721 tggattaatg aaaggatgtt tcacatagtt atatttctgt atatttgaaa agttttactt
     3781 tgatttttaa atagaaagac atttgggtta aattgtgaga taataataaa gaagagttta
     3841 aagcgcacct caaaccactt ccccataagt gtgttacgct ttaaactctt ttatatttga
     3901 agctattaaa aggcatacca tatttttgaa aaaaagtgag aaaaaaggct tacaattgga
     3961 gtggtagtta attagtgact tatttttgat tttatagcac tgatactata aaatatagat
     4021 atcatcatat tacacaatct taatactaac ttaaaaaata tctcctttca caagtatggt
     4081 gataaaattc gttccgggct acctttttag gtagcctact ttaatcttta tacctttctg
     4141 gatcaacgaa agtatacttt atatagtcat aacgccgatg atcgctacgt gcgtccggca
     4201 cgtcagtcac gatatcaaac aaaaagtata catctttctt cattctagtt tttgcagcag
     4261 ggattttaaa gtagttctta ttagaatagt agagattgat taataagcta tcttcgattg
     4321 ctagaaagaa aacttctgaa tcccacacct tataaaaatc tttgataaat ctattcgaag
     4381 gatcaaattt aaaccataat tgtgtctttc cttctattaa aatatcttga aatttactct
     4441 gttaaaaaaa ataaattaat tggtgaaaat gattctccaa taaaaacacc tattgagcta
     4501 agcttaagaa atcggatggg aatactccaa agatcggaaa tagtaaaaga acttttttat
     4561 cataaattta gttatgaaat aagactagat gatacttatg agcattatag aattgttttt
     4621 ttcgtataca attctataga agatgctact gcgactactg cattaacatt cggttttacg
     4681 aaaaatggaa caatcaattc ggataaaacc cgtcaagctg ctacagaaag tgatgatata
     4741 tgtaagaaag tatgcaatgg ggaagagaat tactggatag gggaggaaaa gttgaatgaa
     4801 atctattaat gatttattaa aaaaacatcc tgatttatat aaaaaattaa attctgaaca
     4861 aagaaacctg tccaaacaat tacttaaaat aagattaaaa aaacaaaaaa acgtgacaga
     4921 attctcaaaa atgttaggaa taagtgaaaa aaaatatctt gaatttgagt tcggagatat
     4981 gcatattcct gttgcagagt acaataaact tttgaaacaa gttgaaaatt tgtcctataa
     5041 agattcaatg aatttcacta ttaaggttgc tggcggccct attaaatatg accgtattga
     5101 aaataaacct tatattcgga ataaaagtta ctcagtattt aaaagtagaa agagaaaaga
     5161 agtatcgtca aatatggtct atgctagtgt taattaccaa gatacgagcc gctatattgt
     5221 agaaaaagag gtatatgtac atggataaag gaaatagtgt tacttttgca cttgaaaata
     5281 ttagtttgaa taaaattcat tttgaattaa ctgataattt tagttatgga gatttagagt
     5341 ttaatattga acttggatat aaagaagacg aagcagagta tctagaaaaa aaacttgttt
     5401 ctttacgagc tcacattgta ggggggatgg aaggagcacc ggtagatatt ttactctttt
     5461 cagttattcg tgtttctaca aatgaacagc catataaaaa attagtagaa gaatctatgg
     5521 gagagctagc taaacctatc ctgaataaag cttctaattt gatttctaat ttatatagcg
     5581 aagtaggagt tatgcctaca gttattgatt tatatcagct atatagtcaa caaaaataaa
     5641 atacaagtaa tagagttatc tatttaaatt tgcaagacaa agacctcaac cagtcatcct
     5701 tggttgaggc ttttttgctc tgtaattaac tcgtatagat cctgcaagat agtttgtttc
     5761 tgaataaatg attgatatga agtatttaga tgactgtatt gacaactata aaaatcattc
     5821 gataaaatag tgatgttatc gcatatcttc actatcaccc ataatagtca cactccaagc
     5881 tatgcgataa caggtttgtt gccacacatt ctactggttg attgtttatg gctttatgtg
     5941 gcaacaacca gtacccttag cttagttggt cagagcagac ggctcataac cgtccggtcg
     6001 taggttcgag tcctacaggg tacataattt aatctatttg ataaaaaata aaattcaata
     6061 atggtataat aaaatatcgg ctcgtcgctt ccacagagat tttcgtggga ggaggtgtat
     6121 gatttgtttg aattcacttt ggagcacgtg ctcttgccat tgttaattgg tttaactttg
     6181 gcattattta gtcattggct agataaacat tagagccgac aatggcgctt actgtttcgc
     6241 agcggtaagc gtcttttttt gaaaaagaaa aaaacacaac gtattcgcag gtacgttgtg
     6301 tttatggtgt atatttgttt aaattcactt tcaattatta ttatacttgt taaataaatt
     6361 gtgtcaatat aaaagatatt tttatacgtg tactaaccta tattatcctt ctggtaatgt
     6421 tattccatcg cagcaggata tgaattttac caaaaggata caaaagtgtg gagaaatgat
     6481 ggggatcact gtactggacc acttgatcat cggacgcaaa cgatatttta gtttgagaga
     6541 agaaggaatg atggaagaga agtaagttgc tttcttgcaa aaaaaatgaa gcaagtgtac
     6601 aattatattg taattttgtt tgggaataga agttgaaaca tctgttgcac ctctatcgta
     6661 cacgagcaat gttacaattt aaattagtgc ttttggcacg aggaggatat tattatggaa
     6721 aacggaacag taaaatggtt caacgctgaa aaaggatttg gatttatctc acgtgaagat
     6781 ggaagcgacg tattcgttca cttctcagct atccaaggtg acggattcaa aacattagaa
     6841 gaaggtcaag cagtgacttt cgacgttgaa gaatcagacc gcggcccaca agctgcaaac
     6901 gttgtaaaag cttaatttta tctgtaaatc aaattgatgg tagacatcaa tttttacgag
     6961 agaacaaagc gagtaacaaa aatacctgcc acaagagaca atttctcttg tggcaggtat
     7021 ttttttgtat gaaaaagtaa ataaatttaa gaggctatga aagagccacc ttgtatcaag
     7081 gaataagaac aaacaaaaat ggcttctcat gtttttcatt tgttttctct tatattcagg
     7141 acaaggtgct gatttcataa cctcttattt attcttcatc tggtgtcatt accattggca
     7201 aaatcattgg atgtctttct gtacgttcga ataagaaagg ctgaagcgcg ccagtcatcg
     7261 cttctttcaa tttatattct gaacagtcag gtccattcaa acattctctt aaagcgtgga
     7321 ataagattcg ctgagcttca ttgatcattt ctcctgattc tcgcatgtag acaaatccac
     7381 gagagagaat atctggacca gccaagattt cttttttctt gatatccact gtcgcgacag
     7441 ctaatacaag accttcttct gaaagcaggt gacggtcacg taaaacgaca ttaccaatat
     7501 caccgatccc gctaccatcg atatacacat ctccagccac aaaatgccca gcaggacgcg
     7561 cagaatcgct agttaacgct aaaacatcgc cattttccat gatgaagcag ttttctgcag
     7621 gaacacctac atcttgtgct agagaagcat gtactttcaa catgcggaat tctccgtgga
     7681 caggaataaa atattttggc ttcatcaaac gaagcattaa tttttgttct tcttgtccac
     7741 cgtgaccgga agtatggatg ttattgattt ttccgtgaat cacttctgca ccagcttcag
     7801 acagcaaatt gatcaaatga ttgacacttg ttgtattacc aggaataggt gaacttgaaa
     7861 agatcactgt atcgtctggc tggatttgga tttgacggtg agtaccattc gcgatgcggc
     7921 tcaacgcggc catcgcttct ccttgagaac ctgtacatag gatcatcgtt tcattggcag
     7981 gcaagcgatt gatttcgtgt gcatctacaa atgtgccgtc tggaactttg atatatccta
     8041 aacgttgacc gttgacgatg gcattttcca tgcttcgtcc gaaaacagcg attttacgac
     8101 cagttgccac agcggcatct gctgcttgct gcaaacggaa gatgttagaa gcaaagctgg
     8161 caaaaatgat acgtccttcg atttttgaaa agatcttcaa aatagaatgc ccaatcgttt
     8221 tttctgattt agtaaatgtt ggaatctcag catttgtact atcagataaa aggcacaaga
     8281 ctccttcttc cccgatacga gccattttat gcaagtttgc tggttcgcca acaggagtga
     8341 aatcgaattt gaagtctcca gtcgctacga tatttccaga aggtgttttc accacgatcc
     8401 ctaatgcatc tgggatacta tgggtcgttc tgaaaaagct gacagaagtc tttctaaaac
     8461 ggatgatcgt atcttcattg atctcatgga gctctgcatc tcgaagcaag ccatgctcat
     8521 ctaatttgtt tgtgatcaac gctaatgcga gaggaccagc atagatagga atatttgctt
     8581 gtttcagcaa gtagggtatt ccgccgatgt ggtcttcgtg cccgtgagta atcaccaagg
     8641 cttttacttt gtgtaagttt tgtatgatgt agctgtagtc gggaatgaca tagtcaattc
     8701 caagtaaatc gtcttccggg aatttgatcc cggcatcaat caaaatgatt tcatcttgga
     8761 attgtacccc atacgtattt ttcccgattt cacctaatcc accaatagca aagacgcctg
     8821 tctcgttatt tttgatggaa agtttcatat taaaactcca ctaatgagaa atcagcattt
     8881 tctttttcgt actctagatg acttccttct agaagttgaa cgaattcaat attgtatggg
     8941 gtattttctt ccacacgttg gcgagcgtct acatcgcttt ttgcctcaat gtaaagagat
     9001 tttgtttctt cccgtttagg gttgcgtact tttgtttctt gataataaac tttatagatc
     9061 attgttttgt ctcctttatc tgcgtaaagc agtgatttag ctagttgatt tttttagaca
     9121 acacaaaacg aggggacaag ccacctggtt tttcatatgc tttgactaga atactggatt
     9181 cgagtcaaag agacaagcct cgtttttgct tatctgttcg gatatttttt caaccgtacc
     9241 ttctgtataa aaaatataca acaattaatt gttattctag cataaaacag caaataagta
     9301 tagaaatcaa ctagattgaa agaaaaaatc aatgtttatc atcccgctat aatcataatg
     9361 acttttttat gagagaagtg ttctattaat aggctcgttg tgctatcatt tttagtaatg
     9421 aaaatgtaac aaaagattaa aaggagatga gaaagtgaaa ctaatgtggc attataccat
     9481 gcgttataag aaattcttgt ttttcaattt catatgcgta tttggtttta ttttgattga
     9541 attaggactt cctacgattt tagcacgtat gatcgatgta ggaatccgca atgatgattt
     9601 tgattatgtg aagcaacaag ggatatttat gatcgttatt actgtaatcg gtattatttt
     9661 gaatatcttg ttaggttatt ttggttcgag gattacgact aatatcgtag cagatattcg
     9721 agatgatttg ttcgaaaaga tccaaagtta ttcgcatcag gaatacgaaa atctaggcgt
     9781 atcttcattg atcactcgta caacaaacga tgcttatcag atcatgttgt tcctgcaaaa
     9841 tattttacgt atcggtttta tggcaccaat gatgttcatt gtcagtttgt acatggtcat
     9901 gcgtacaagt gtgacactca gtttgtatgt agtaggtgca ttacctttac tattattggc
     9961 agtcgtagcg attgctaagg tgtctgaacc gttatctaaa aagcaacaaa aaaacttaga
    10021 taaaatcaac agtatcttgc gggaaaatct ttcaggattg cgtgtgattc gtgcattcgt
    10081 caatgaaaag tttgaagaaa aacgttttgc tgtagtcaat gagaattatg cttctagttc
    10141 aaaaagctta ttccgtttga tggctgttgc acaaccgggt ttttatttct tatttaatat
    10201 cgtaatggta ttgatcatct ggagcggggc agtgcaaatc gatgcaggcg agcttcgtgt
    10261 aggagatttg attgcgttta tcgagtatat attccatgcc ttgttctcat tcatgttatt
    10321 tgctagtgtg ttcatgatgt atccaagagc agctgtttca gctggacgta tccaagaagc
    10381 cctagatgcc aaaccttcga ttgtagaaga tccaaatggt atatctgata caaaaactaa
    10441 agggtatatc gaatttcaaa atgtaacatt tgcttatcca ggccattcgc aagagccagt
    10501 gatacgaaat gtcagtttta cagcctctcc aggagagacc gttgctttta ttggaagtac
    10561 cggtagcgga aaatctacgt tgatccaatt gatccctcgc ttttatgatg tgagtgaagg
    10621 aaagatcttg atcgatggag tagacgtgag agattatcgg ctaagcaagt tgcgagataa
    10681 gattgggttt attccgcaaa aagcgctgct atttactgga acgattgcag ataatttgcg
    10741 atacgggaaa gaagatgcta cgcaagaaga aatggaacgt gcagctgaga ttgctcaagc
    10801 ttcagaattc atttctcgaa aaccagatgg atttaatgaa catttatcag aaggtggttc
    10861 caatttttca ggtggacaaa aacaacgatt ggcaatagcc agagccatca tacgccgtcc
    10921 ggaaatctat atttttgatg acagcttttc tgcattagat tatcaaacgg atgcgaagtt
    10981 acgtgctcgt ctgaaaaaag aaacgacaga atctactgta ttgatcgtag cacaacgtgt
    11041 gggaacgatc atgcatgcag ataagatcat cgtattaaat gaaggagaag tcgtcggtat
    11101 ggggacgcat cgggagttgt tggaaaaatg cccgatttat tatgatattg ccgcttctca
    11161 attatcaaag gaggaattag catgaaaaaa accttttctt ccttaaaacg tttagctcga
    11221 tatatcagac cgtatcgtga gacttttctt ttagtgatcc tatttacagt tctgacagtg
    11281 gctttcaacg tggctttgcc atatgttaca ggacttccta ctacagaaat cagccgtaat
    11341 attgccaatg gcgaatcaat caatttttct tatatattca aatgtctcat ttggatcaca
    11401 gttgttggaa tagggtattg tatttcgcaa ttactttctg gtgttttgat gacaaatgtc
    11461 gttcaaagtg ccatgcatga tttgagaaga gacatagaag aaaaaatcaa tcgtcttccg
    11521 gtttcatatt tcgataaaaa ccagcaagga aatattttgt ctcgtgtgac gaatgatgta
    11581 gatgcagtca gcggtgcttt acaacaagct tttattggta tcgtaaatgc gatattggga
    11641 attacgttgg ctgcagcaat gatgttttat atccagccga tgatggcgct gatctctatg
    11701 atcatgattc caacatcgat ctggatctca aaacgagtga tcaatgcttc gcagaaatat
    11761 tttcaaagca tgcagaattc tcttggtgag ctaaacggct acgtccaaga aaatatgaca
    11821 ggttttagtg tattgaaagt atatggacgc gaaaaagaaa cattggaagg attcaaaaaa
    11881 gttaatcaca gcttgaaata ctttgggttt cgtgcagcat tcatttctgg cttgatgatg
    11941 cctctagttc aattaactgc ctatgctact tatattggta tggctgttct aggaagcttt
    12001 tatgttatta ctggggcgat cgttgtcggt caactacaag cattcatcca atatatttgg
    12061 caaatcagtc agccaatggg gaacgtgaca caattgtctt cagttttgca aagtgcatca
    12121 gcggcaacga aacgtgtatt cgagatttta gatgagccag aagaaaaaga aaacaaagta
    12181 gatgcttcac tgccagaaac gattttagga gatgtgacat tcgatcatgt cgattttgca
    12241 tatgatccga agaaaccatt aatccaagat ttgaattttg aagtgaaagc tggtcaaacc
    12301 gtagctgtgg tcggtccgac cggtgccggc aagacgacat tgatcaatct attgatgcgt
    12361 ttttatgacg tagacaaagg agcaataagg attgatggaa tcgacaccaa atcaatgagt
    12421 cgcagtgatg tgcgttcgct atttggtatg gtcttgcaag atgcatggct ttatcaagga
    12481 acgattgcag ataatatccg cttcggaaag cttgatgcga cagattatga agtagttgat
    12541 gctgcgaaaa cagcaaacgt agatcatttt atccgaacga tgccagatgg atatgatatg
    12601 gccatcaatt ctgagggaga caatgtgtct ttaggacaaa aacagttgct gacgattgcc
    12661 cgagcagtgg tctccgatcc taaaatcttg attttagatg aagcaacaag ttcagtagac
    12721 acccgtttag aagcgttgat tcaaaaagca atggatcgcg tgatggaggg acggacaagt
    12781 ttcgtcatcg ctcaccgttt atcgacaatc cgtgatgcag atctgattct agtgatggat
    12841 caaggtcaga tcattgaaaa aggaacgcat gagagtttat tggcacaagg tggattttat
    12901 gaaaaactgt ataacagcca atttgctgag gaagcagaat aagtcatata agaataaaac
    12961 agtttaaaac ttattttttg ttctctattc ttgataatgg taaagtaaag ttatcaagaa
    13021 aagtgaggac acggagatgg aacagttttt aaatgaaaaa attactgaag ccaaaagaga
    13081 agttgatcgt ttaacgcacg tctgtcagac gaagcctttt gattcagctg atttttttga
    13141 aaatgagttt caaatcgaac gcttggcagc acaaatagaa gtgtacgaat taattttaaa
    13201 aaaagtaata gaagaaaata tagatgtaca ataaaaacag gtaaaacgtc agttaccaga
    13261 tactgtaaat agacatctgg cctgagcagg ttttacctgt tttttttctt atgaaataaa
    13321 tatatgctat gataccttta ttcaatagga ttagtttcta caaaaaatga aaggagaaaa
    13381 ataaatatgg aacggtttat agcatcaaat gccacagtga ttggtgatgt gacattgtca
    13441 gaagatgtga cgatctggta tcaagctgtt ttacgcgggg acagtaattg gataaaaata
    13501 ggacaaagaa cgaatatcca agatggtaca atcatccatg tcgatcatga tgcacctgta
    13561 gatattgcgg agaatgtgac ggtaggacat cagtgcatgc tacatggctg tacaatcgaa
    13621 aaaggggcgc tgatcgggat ggggaccacg atattgaatc atgcggttat tggtgagaat
    13681 agtttgattg gtgctggatc attagtaaca gaaggaaaag ttattcctcc taatgtcttg
    13741 gcttttggtc gcccagctaa agtgatccgt cctttgacaa aagaagaaat acaaaaaaac
    13801 aaagaaaaca tccagcatta tgtcgagata ggtcggatgc atgcgcaagg aaaattcaag
    13861 gagcaaagat aggcagtagc atccataaaa taaaaaagga tggtcagttt gagttgacca
    13921 tcctttttta tttggtttag ataagagagc ttaaaaaagc ttcctttgaa acagattaat
    13981 ttaatgaaga actgttttcc atgacttcat cgatcaatcc atatgctttt gcttgttcag
    14041 ctgtcatata gttgtcacga tctgtatctt tttcaatcac ttcaagcggt tgtccagtgc
    14101 gttcagctaa aattttgttt aagcgttcgc gtgtttgcaa aatatggcgt gccgcaattt
    14161 cgatttcagt tgcttgccct tgagcgccgc caagaggttg atggatcatg atctcagcat
    14221 ttggtaaagc aaatcgttta ccttttgtac cagctgtcag caagaagctt cccattgaag
    14281 cagccatacc cataacaatt gtttggacat ctgctttgac aaagttcatt gtgtcgtaaa
    14341 tagccatacc tgctgtaact gaaccaccag gagaattgat ataaagatag atatcttttt
    14401 cagaatcttg agcgtctaag aaaagcaatt gggcgatgat cgaattagca agatcatctg
    14461 ttacttggcc gcttaacatg atgatacggt ctttcaataa acgtgagtag atatcgtaag
    14521 ctctttcacc tcgtgaagat tgttcaatga ctgtaggaat taaattcata atagagttcc
    14581 tccttgattt cgtttagaaa tagtgtacca taatttagtt tctaatacct aaaatgtagt
    14641 atacattttt ggtcaaaaaa ggtcaaataa aaaacttagg caaattagtc gcataaaccc
    14701 gctttatggt atgataagca gagaaacaaa acaaggagga acaacagatt gatagttggt
    14761 attactggcc ttatcggagt gctgacaggt gctgtagttg tctatatcgg catgatactg
    14821 caaatgcgtc acttggaaaa taaagaacag cgtagacgtg aattggaatt gaaagtcaaa
    14881 gaaatcgaga cgctgaatca attaaataaa aagatcaacg agatcttgca aaaacggaca
    14941 acgctgatgg aagaatatgt cagctttgat gcatttgatg attgttatat cacgattgac
    15001 gattttgcgt atctccaatc attttcagcg caaaatagtt tttatctgcc aaattatatt
    15061 ttagacgagc tatttaagaa tatctcccat cgaaaagtga ttttatctcc agatgaaaca
    15121 atgaagattg gtggatatgc ttataaaggc ggacgcgtga ttttagagaa ttttacggat
    15181 agtctgactg aaatgatcaa tgaaaaaaaa gctcagttga agactatgac gcgccagccg
    15241 attggtttct tctcgaaaga tgatgtttgg agctgatatt ttgaagcatt gccgttgcac
    15301 atgagagcgt ggtaatgctt tctttttttc ttaatgatac aatagaactg aacggagtga
    15361 agaaagatgt tacaaatcta tgcccatcgc ggaagcagcg gcacccaccc agaaaatacg
    15421 cttcctgcat ttgcagaagc tgtacgagta ggtgcagatg gtatcgaact agatgtccat
    15481 ctaagcaaag atggttattt gatcgttatg cacgacgaag aagtggaccg gacgactaac
    15541 ggtaaagggc tgattcgaga aaaaacattg gaagaactca aaaaattgaa tgcagggggc
    15601 aaattcagca aagaatttcc agcagctaaa gttcctgaat tgagagaagt gttaggactg
    15661 ctcctccaaa aaaattaccg agggatgcta atgattgaaa ttaaaactga tcagtatgaa
    15721 tatcccggta tcgaagaaaa attagccgaa acattaaaaa gtagagattg gccttttcaa
    15781 catgcatatt gcagctttca tttggattct ttggaaaaaa tcgcattact ggaaccggag
    15841 atacagctgg acttattgat gagtacttct gataaaaaga tccaactagc agcagaaacc
    15901 ccttatatag aaggagtaca tccaaaaaat acttggttgt tcgaacatcc agtccaggca
    15961 gctgattttc caaaagcaat tcgtgcatgg acgattaacg atgaagaaga aatggccaaa
    16021 tgtcaagaac tcaaaataga tggaataata actgattttc cggagaaagc tcttcggtgg
    16081 aaacaatcaa cgaaaagtag gtaaaatatg gaaaaagtat tagtgatcgt cggtccgaca
    16141 gcagtcggta aaacagcgct aagtgtggaa ctcgcacaac tatttggagg agagatcatc
    16201 agtggtgatt cattgcaaat ctataaaaat ctggatatcg gtacagcaaa aatcaaagaa
    16261 gaagagatga ataatgttcc gcatcatttg attgatgtga tcgaacctac tcaacgttac
    16321 tcagccgctg attttcagca ttcaggtcga caattgatta ccgagattgc gaaccgaggg
    16381 cacctaccta tcatagctgg tggtacgggt ctctatatcc aatcccttct atatgattat
    16441 cagttaggag cgacagaaga agcgaatcct gagattagaa aaaagtatga aacgttagct
    16501 gaaacaattg gttcccaagc attatggaat cagttgaaag aaaaagaccc ggcagctgct
    16561 gagaaaatcc actggaacaa tcaacgcaaa gtgataagag ctctggaagt tttagagacg
    16621 acaggacata gtatcacggc acctaaagaa gagccaaagc aattgtatga ttacctcatg
    16681 attggattag atacgaagcg tagtattctt tatgaacgaa tcaatcaaag agtcgatttg
    16741 atgctagcag aaggtttagt agcagaagcg cgtaaggtgt atgagcttgg agaagtccag
    16801 gccagtcaag gtattggata taaagaattt ttcccttatt ttgaagggaa agaaacattg
    16861 gaagaagcaa cagaacaagt caaactacat tcgagaagat acgcgaaacg tcagttgacg
    16921 tggtttcgta accgtttgga tgcacagtgg tttgatctta taagtgagcc aaatcaaaaa
    16981 agtcagattg agcaaacaat caaaaaatgg ctggaggcgg aataaaatgg aagaaaaagt
    17041 gatcatcgta ggggtagaaa ccgaagcaaa ccagcgttac ttttcagagt caatggaaga
    17101 gctagttcag ctgacgaata ccgcatcagg agaagtcgta ttcacaatta cacaaaagag
    17161 accacaagtg gatcggcaaa cgattatcgg taaagggaaa ttacaagaat tagttcaaca
    17221 agcggatgcg catgaagcgg atttgatcat ctttaatcat gagctaactc cgagacagag
    17281 ccaactgatt accgatgctg ttggtcttcc ggtaattgat cgcgtgcagc ttatcttaga
    17341 tatttttgcg atgcgtgctc gttcgaaaga agggaagctg caggttgaac ttgctcagtt
    17401 agaatacctg ctgcctcgtt tagtagggca agggaaaaca ttatctcgtc ttggcggtgg
    17461 gatcggaaca agagggccag gtgaaacaaa gttagaaaca gaccgccgac atatacggaa
    17521 taaaattttt gctgttaaga aagaattgaa agaagtagaa gctcatcgag agcgcaatcg
    17581 ccaaaaaaga aaaaatagcg agatttttca gattggactt attggctaca cgaatgccgg
    17641 gaagtcaact atcttgaatc tattgacaca agcggatact tattcgaaag atcaattgtt
    17701 tgcaacgtta gatccattga ctaagagatg gcgttttgct gaaggatttg agattactgt
    17761 aacggatacc gtcgggttca tccaagacct gcctacgcaa ctgatcgatg ctttccattc
    17821 tacattagaa gaaagtcaaa atatggatct tttgctacat gtagtcgatg ctagttctcc
    17881 agatcgtatt ttacaggagc agactgtctt aaaattgatg gatgaactga atatgaaaga
    17941 aatgccgata ctgactgttt acaataaggc agaccagatc gacccagcga tgttcacgcc
    18001 tacgctattc ccaaatgtat tgatttctac tcagacgaaa gaaggtaaga tggcgttgat
    18061 cgaagcaatc aaacgtcagc tgatggaact aatgacccct tatacacttt ctgtgccaag
    18121 caacgaagga cagaaattaa gcgagttgag aagacaaaca atggttttgg acgaagcgta
    18181 catggaagaa acaaacgaat atgaagttaa agggtttgca aaaaccaatt caaaatggtt
    18241 aagaaaaaac caataaaatt ttattttttt aa
//
LOCUS       DAFEOM010000054        17107 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.056, whole genome shotgun sequence.
ACCESSION   DAFEOM010000054
VERSION     DAFEOM010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.056"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            7..1398
                     /locus_tag="LP524_001986"
     CDS             7..1398
                     /locus_tag="LP524_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBL2235015.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIETSGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            1414..2454
                     /locus_tag="LP524_001987"
     CDS             1414..2454
                     /locus_tag="LP524_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL2235016.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            2473..3558
                     /locus_tag="LP524_001988"
     CDS             2473..3558
                     /locus_tag="LP524_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HBL2235017.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            3591..4553
                     /locus_tag="LP524_001989"
     CDS             3591..4553
                     /locus_tag="LP524_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBL2235018.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            4546..5979
                     /locus_tag="LP524_001990"
     CDS             4546..5979
                     /locus_tag="LP524_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBL2235019.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKGELI"
     gene            5976..7046
                     /locus_tag="LP524_001991"
     CDS             5976..7046
                     /locus_tag="LP524_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235020.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            7033..8454
                     /locus_tag="LP524_001992"
     CDS             7033..8454
                     /locus_tag="LP524_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HBL2235021.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            8467..9474
                     /locus_tag="LP524_001993"
     CDS             8467..9474
                     /locus_tag="LP524_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL2235022.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            9486..10490
                     /gene="neuB"
                     /locus_tag="LP524_001994"
     CDS             9486..10490
                     /gene="neuB"
                     /locus_tag="LP524_001994"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HBL2235023.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            10487..11635
                     /gene="neuC"
                     /locus_tag="LP524_001995"
     CDS             10487..11635
                     /gene="neuC"
                     /locus_tag="LP524_001995"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HBL2235024.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            11608..12285
                     /locus_tag="LP524_001996"
     CDS             11608..12285
                     /locus_tag="LP524_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBL2235025.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            12275..13417
                     /locus_tag="LP524_001997"
     CDS             12275..13417
                     /locus_tag="LP524_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBL2235026.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            13430..14407
                     /locus_tag="LP524_001998"
     CDS             13430..14407
                     /locus_tag="LP524_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL2235027.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            14407..15315
                     /locus_tag="LP524_001999"
     CDS             14407..15315
                     /locus_tag="LP524_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HBL2235028.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            15316..16068
                     /locus_tag="LP524_002000"
     CDS             15316..16068
                     /locus_tag="LP524_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HBL2235029.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            16073..17020
                     /locus_tag="LP524_002001"
     CDS             16073..17020
                     /locus_tag="LP524_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBL2235030.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT         6164 a         2190 c         3090 g         5663 t
ORIGIN      
        1 gtatatatga ataaaaatgg agaatggagt gctgcacgac ggatattcat tatattaatg
       61 gatgttattg tatacaatgc agcaatatat agtagtttct tactgaagtt tcgtggagag
      121 atcccattac gaaatttaga aactttccaa cactcagcaa ttattatttc agtgattttt
      181 attgcgttga acattttgtt gggtgcatat gttttttata atcgaatgat cagtgacatc
      241 gtgtttgtca cagtcatcgg tcaggtattg atgacactag ggatcatggt agtgactttt
      301 gcgggccgct ggtttgcttt tccacgtctt gttatcttac tttcatttgt tctcggtacg
      361 cttttcttaa gtatctatcg tattctcgta tataaacttt atttacgcgt aagtcgagat
      421 aaaaaggtcg tcattattgg gttagaaaag gatgtcgcac cagcaatcca aaattttggt
      481 tcaaagaaaa acaacaaaca taaagtcgaa gcagtcattc ttgataacta ttatgcaaac
      541 atcaaaaata tgattaaaga catcgatatt gtctatcttg cttctcatat cgaagaaact
      601 gaaaaaatca aaatctatca attagttact aaaaatgaaa aaaaattgtt tttgaataca
      661 acatttgaaa acttgacatt gatcaatcca aatattatga atttcgaaga tgaaagtatt
      721 attgaaacat ctggatttag aatccaacct gaatatgaac tattcaaacg tttgtttgat
      781 atcatcgttt cacttgtttt attagttatt gcttcaccat ttatgttatt cacagcaatt
      841 ttagtcaaag tgacatcacc gggaccaatc atctataaac aagtgcggat cacaaaaaat
      901 caaaaggaat tttcaatcta taaattccgc tcgatgaccg ccacagccga agcaaaatct
      961 ggaccagttt tagctaagag caatgatgca cgagtaacgc ctgtaggtaa atttattcga
     1021 gctgtccgct ttgatgaatt accacaaatc ttcaatgtgc tgaaaggcga tatgtcgatc
     1081 gtaggaccac gtcctgaacg tcctttcttc gtcgatcagt tcaatgcaga aaacccttat
     1141 tactacttac ggcataatgt tcgtgctggt atcactgggt atgcacaagt ctatggaaaa
     1201 tatgtctctg attacaacag taaattacgt tttgatttac tttatatcaa aaaatattca
     1261 ttgatgcttg atttaaagat cttgctgcaa accatcaaga tcctatttga taaaatgtcc
     1321 tctcaaggac ttgaagaaga cgaaaatgtg atctcattgg cagaaattgc ttttccgaat
     1381 gaaaaaatct ttcgctagtt gggaggcgta aaaatgaaca atccgaaatt cagcataatc
     1441 gtaccagtat acaatgcaga agcaactatt ggtcgaacga ttgatattct aaaagaattg
     1501 gattatgaag agtacgaagt cgttttagta aatgatggat caacagatca aacacaggaa
     1561 atcattcaag aactgattga aaatgatgaa cgttttgaat tgattactac gaccaatcaa
     1621 gggcccggat tagcaagaaa cgaaggaata agacagtcaa aaggagaata tctcttattc
     1681 ttcgatgcag atgacacgcc agtaaaaacg atcttgtcag attatgatca tttgctagaa
     1741 aagagtccaa aagcggatct tgttgttagt tcgtttgttt ttcgtacaat ggacgaggga
     1801 agaatcgttt ccgaaaaaga aaacttagtt acagaatgtc actatctaac tcacgaagag
     1861 tttataaaag acatgtatca gttaatgaat gaacagttga tgtatgttgt atggaacaaa
     1921 tgctatcgta gcgatattct gaaaaataac caaattctat ttaaaactta taatagttgt
     1981 gaggatcgaa tatttaactt acattattat gaatactgtc atcaagtgat catgaaccca
     2041 aaagtagaat atatttatga atttgaaggt gggaagggga tcactaatca ataccgacct
     2101 aacaagttcg aaacatttaa agaattctat tcattagcaa atactgtaac agacgatatt
     2161 aataaagacg gtatggcggc gcttctttta aaagggacaa cttcagttat tttttctatt
     2221 tatagtacct ctgcaagaac ttcaaaagag aagaaagacg aagcaaagca gatattgagt
     2281 gatccagtga tccgagaagc caagcaaatt gctaagacgg attcttcagc aaaaaaaata
     2341 acaaagttgc tttacaatat gccagctcca ctatttttat ctgctgtaaa agttggaagc
     2401 ttcgttgaag tgaaaatgcc agggttaatg gctgctttga agcgagtata ttagttaatg
     2461 gaggaaaata taatgaaaag aattttgcat tttcagggac gaatgggttt aggtggagca
     2521 gagtctttca tgatgaattt atatcgtaaa attgatcgta caaactatca atttgatttt
     2581 ttgatttatg aagattatgc agatgttcaa gactatcatt cagagattga aagattagga
     2641 ggccgtattt ttgttgtacc taatcctaag aaaaatattt ttaaatattt aatagaagtg
     2701 aataagttac taaaaaaaga atctttttct atagttcaca atcaggttta ttttggcggg
     2761 ggaattaatt tatggttagc caaaaagaat ggaatcagac agagaattgc tcatagccat
     2821 gcaactgaag atggaaaatc tcaaaatatt gtaatgaatg tattgcgtaa atttttaacc
     2881 aaattgttgt tacaaaatgc aacagactac cttgccgtta gtcaagaagc tggtgaaagt
     2941 ctgtttcaaa accatccctt tgagatagtt cataatggta ttgatttaga gctttatagt
     3001 aaaaattcag aagctaaggt taataagaga aaagaattgg atatctcaat gagtactttt
     3061 gttgtgggaa acattggacg attagagaaa caaaaaaatc aatcttattt gttagagatt
     3121 tttttagaaa tactaaaaat gaattctgat tcttacttga ttttagtagg tgaagggtca
     3181 cttagaaata aactgaaagc caaagcagta gaattggata tagagaaaag agtaaaattt
     3241 ttaggagaaa gaaacgatat tcccgagtta ttatctgtga tggatgtttt tgtaatgcct
     3301 tctctttatg aaggtctgcc aatggttgga ttagaagcac aagcagcaaa attaaaatta
     3361 gtcttagcag atactatttc caaagataca aagttaacaa ataatgtgga ttttgtatca
     3421 ttagatgaaa caccacaaga atgggcaaaa aaagtaataa aagagccgta tgctaataaa
     3481 ataacacaag aactaaagag ttatgatgtt tcgtacacta ctgaacaaat ggagagtata
     3541 tatagtagag atcattaaaa atgttcaaga tattttagga gtgattattt ttgaaaatat
     3601 gttatttgat attggcatac aatgatattg gcaacttaaa gagattggtc aatcgattga
     3661 acgaaaatgc agtttttatt attcatatag ataaaaaaac tgatatagaa ccatttattg
     3721 aagcatttaa atcatatgat aatgttgaat ttttatgtaa taaaaaccgt gttaaaattt
     3781 attggggtgg tttttccatt gtacaagcag aaatcaattt agtaaagaga gcattacaaa
     3841 atgagaaata tttaaaatat gtattattat ctggtgctga ttatccaatc aaagataatg
     3901 agtatattta taattatttt aaaaagaata gttcagtgga gtttattcgt ggaatagatc
     3961 tagatcaaat taaacataaa gaattttatt ataaacatat agatgtatat caaaaacatg
     4021 attatcctag aattaatagg aataacacaa ctgcatttaa aatttttaga gctataatta
     4081 atcgttgtct gaggatgatt aaacttcccc caaaaattag acaccataaa tttgatttgt
     4141 atcatggttc tcaatggtgg gctttgtcga aagaatgcct aacagaatta atacaaatgt
     4201 atgaacaaaa tcaacaagac tacctgaatt ttaaaatagg tatgtttgcc ccagatgaaa
     4261 aattttttca tacacttttt tttaattcct cgttcaaaaa taaaaatgtt attggaggac
     4321 cagatttacc acttgaattg aaaaatattg aagaaactac tttacaaact tcgaaattag
     4381 caaatattca tattattgat ccttccatga acaaatggtt tgatgaaagg gatatagatc
     4441 aaataaaaga aagtgaaaaa ttatttgtaa gaaaagttaa atcggattac tctgataaac
     4501 tattaaatag aattgataaa gaaattttga aagttggaaa tatagatgaa tagaatagta
     4561 cctgttttag agaagagttt attaagtcga gatgcgtttc gagtaataaa tttaatcatt
     4621 tgtctgcttt attttatgcc tagtactttt aatgtaacta atatcccgat gaaaatagct
     4681 tttgcttggg gtggaattat agttttgtat gaggttttta taaagaaaaa tttttttaaa
     4741 atgcgttttt cttggctact gattggagca atgattagtt ttatcttaac tataatcatt
     4801 aacagagaaa attttttaat tccatctatt tataattttg gatatttgct gattactttg
     4861 atagtaattt ttcctgcaga cttttcacaa agtgaagagg aaaaaaagaa aaaaatggtg
     4921 tattttaata atgttttcat tatcataatt tttttagctg cgctaatatc tatctatcaa
     4981 tttatttttt tgatttctta tcatgtccca acaggaacgc caggattact tgcaagacaa
     5041 ggttttattg aacatcggtt atttggtgtc tatacaagtc caaatgttgg agctatgttt
     5101 ggatatactt ctattatttt aagtttaatt agtgtattaa tttcaaaaat aacaaaaacg
     5161 attcgtgttt tttatatgat gaattttatc gtacaaatat tttatattac actttcttcg
     5221 tctagaggtg cccaggtagt aatttgttct tttttagtat tatttctttt gttgtttgcc
     5281 aattcaaaat ttagaaagag cattagagaa gtactacctg taaaaatcaa atggatggta
     5341 agcatattat tagttctatt ggtatatttt tcttttggta ctaattatgc taaggatttt
     5401 ttagcaacaa ttcctgtatc gattgataga aaagtagaag ttatagagtc aaatgaatca
     5461 gataaaactc cacttccaga aaaagaagta atattacagc attctagtga taactccgaa
     5521 atttcagctg gtcgttttac gatttggaaa gctgcgctta aagttataag accaactttg
     5581 ttatttggtt atggagagac cgatttctat gggaatagcg atgcaaaatt tttgaaaaaa
     5641 gtagatcttg atactacaga tgtgaatgaa ctaaaaagag cacatggaaa catgcataat
     5701 ggatttcttc aagtacttgt atcaagtggt ataattgctt ttatatgtat ctatgctttc
     5761 tatgtgttaa atatctttag tttactcaaa acatttttta gaagtaaagt gaattatgcg
     5821 tttcttggta ttgttttaat ttttatacta tctattttta tagatgaaat ggtggaggct
     5881 catgtacttt tcaataagcg cgatgtcatt tcaattgttt tctggtatta tttgggaagc
     5941 atttcttact tgtatttaaa aaaaggagaa ttaatatgaa gacagctttc attgcttgga
     6001 ctcctttaca tttgattaat atattaaatg tttatcaaaa tttttaccga gaatcaaaag
     6061 gagatttatt tatttattct gaatttgaaa acgctagaag atatagtgag cgtataaaag
     6121 aattagggct atttaatgat gttgtttttg tgaatcaccg gaaaatcggt tcttccttta
     6181 aacgtaaaat taacttgtta actaaccata atcaattttt ctcggatgat ttatccgttt
     6241 atgatgaaat ttttatacag ggggaaaatt atttcacaaa aatacttttc tccaatgttt
     6301 acaaaaaaaa tccagatgta aaactaaatt atatagaaga tggtttgggt acttatttga
     6361 agaagagtat attttcaaca agtaaaaacg ttcaaagagt ttttaaaata ttaaataaaa
     6421 atagcatctt caaaaaaaaa attgacgatt attttgtcta ttgtccggaa ttgttacttt
     6481 atgatgtaaa aaatataaaa aaaattccca aaatagatga aaacagtaaa ttgatgaaaa
     6541 acatacaatc aatctttggg atagaagaag tttttgtcgg agataaatgt atcttttttg
     6601 atcaaccttt attatctgat ggttatggca tagatgaaac agatattttc gctaaagtaa
     6661 atagtatatt ttctggaaaa aaagaaactt taattaagct tcatcctaga gcaaggacaa
     6721 atcgttatgg tgaaaaaaac atattgaatt ccagtttacc gtttgaagtc ttgctacttg
     6781 aaaatgactt ctcttcaaca cttttgatta gtcctatatc tacaatttct ttttcaccat
     6841 ctatgatgtt tgaaaagacg atcaactcta ttatgttggc taaattaatt ttaaatgaat
     6901 atacagcatt atctgaagat aaaaaagaaa cattgacaaa tattgttctt ttctgtgaac
     6961 attataaccg tctatcagaa gaaaaaattt tactcccaga aaactgggca gaattaagaa
     7021 agttggtaga gtatgggatt atttaacaca ctttccaagt caaaattagt taagtccagt
     7081 ttctggtata ctatagggaa tttttttatt aagggaattt cattcataac tattcctatt
     7141 tttgtaaata ttactactca agcagaatat ggtttgatta ataatttcac ttcatttgta
     7201 tcaatttttt cgattcttgt tgggctaagc ctaaattcgg caattaataa tgcaagtttt
     7261 gactataaag atgacataaa tgaatttatg tcatcggtac tttctctagc tacactttcg
     7321 ttaattgtgt tcttattaat agggaacata tattttttgt tttcaaatgc tttttttgat
     7381 atgaaccaac ttgtatttaa tttgatgtta ttacaaagtt ttgctagttt tgtaattagt
     7441 tatatccagt cttattttac gattaacttt aaccatttta aatatttgat tttatctttt
     7501 atatctactt ttttgaatat tgcaatctct gtagctttga tgcagacatt atattttgat
     7561 gataaatatg ttggcagagt agtcggcgga acactgtcat ttgtgttgat aggtttagta
     7621 atttatttaa gaataatgat aaagggtgct catttttttg ataaaaaaca gtggagatac
     7681 gctttaaaaa tctctttgcc gctaatccca cattccttag caaatatagt tttagcacaa
     7741 ttcgatagaa ttatgattaa ttcttattta ggtcctgctg cggggggaat atattcctac
     7801 attagtaaca taggtattat tctaagcgta atatggatgt ctactaataa cgcctgggtt
     7861 ccatggttct atggtgagat gagtaaaaat aatgaaaaaa aagtgaagaa agttagtaac
     7921 tattatttag tagtctttac tttgattacg atgattgtaa tggtagtttc gattgattta
     7981 gcaaaattta tggctccgaa agactattat tctgggttat ctttagtggt tccgataacc
     8041 ttaggctatt tctttcagtt tttatatagt ttaccagtta atgctgaatt ttatgagaaa
     8101 aaaacatcgt ttattgcgat tggtacagta gcaagtgcta tagttaatat actattaaac
     8161 atgattttca tacctaggtt aggaataatt gctgctggat acacaaccgt atttacgtat
     8221 tttttacttt ttatttttca ttataatatc gctaaaaaaa ttacgaataa acaattattt
     8281 gacacaaaaa aaatatgtat cataataatt tttgtatcaa ttttttcttt gatacttcat
     8341 attttaatca atcaaatgtt gattcgttac gttttgacat cattattgat aattagttta
     8401 gttatatatt ttttaaagat taagaatcga attatagatg actataattc ataggaggag
     8461 atataagtgt ttaaaggaaa aacaatatta gtaactggcg gaactggttc cattggaagt
     8521 gaaattgttc gtcagctttt aaaatatgaa ccaaaagcta tcagaattta tagtagaggt
     8581 gaagaaaaac aattttatat gcaacaagag ctctcaaatc acactaattt gagattttta
     8641 attggtgata tcagagactc tcaaaggatt gattatgcat gtcgtgatgt ggatattatt
     8701 tttcatgctg ccgcaatgaa acacgttcca gcaagtgaat ataatcctat ggaagctgta
     8761 aaaacaaatg tgatgggaac tcaaaatgta atagatgcag cgatcaaaaa taatgtaaaa
     8821 cattttgtag gtatcagtac agataaagta gtgagtccta ctaacaccat gggtgcgaca
     8881 aagttattga gtgaaaaatt aataatagct gccaatcagt ataaaggaaa aacagaaaca
     8941 atattttctt gtgtaaggtt tggaaatgtc atggggtcaa gtggatctgt tattcctctt
     9001 tttagtaaac aaataaaaaa caataaagat attactttga cagatgttga tatgacacga
     9061 tttatgatgt caataccgga tgctgcaaga ttagttctgt ctgctgctat gaattcaaaa
     9121 tttggagaaa catatgtctt aaaaatgccg gcaatcaata ttttaacttt agctcaatca
     9181 ttaataaaaa tatataatga tacacatgat gaaaaatata gaggtgaaat aaaagttgta
     9241 ggtatcaggc caggagaaaa attatatgaa gagctaatga ctacagacga aattgaacgc
     9301 tcatatgaaa atgataaaat gtatatcatt ccaccacact atcatgctac tgacgaactg
     9361 tacaaagatt atgaaaaaat taaaatcact gcatactctt cagatgcaac agaattaatg
     9421 agtggacagg aatttattag aatcgcaaaa gagaataatg tcgtgacttg gtaaggagtt
     9481 ttatgatgaa atcagaagta tatgtaattg cagaaattgg tgtaaaccat aatggaagtc
     9541 tagatttagc tttagaaagt attgataaag ctaaagaatg tggagctaac gcggttaaat
     9601 ttcaaacatt taaaacgaat aagctagttt ccaaaaaggc tccaatggca aaatatcaga
     9661 aggataatct taaagaggac agttctcaat ttgaaatgct aaaaaaatta gaattatctc
     9721 aatctgattt tattagaata aaagattatt gtatacaaaa taaaattgat tttttgtcta
     9781 ctccttttga tgaagaaagt gcagactttc taaaacaaat tggtgtacaa ggatttaaaa
     9841 ttggctcagg agatttaaca aatttacctt ttcttagaaa aatagatatg tatgggttgc
     9901 cagttttatt atctacaggt atggctgata ttcatgaggt cagtgaggct gtagattgtt
     9961 tcatagatag tccagttact attttacatt gtacttcaaa ttatccggct agttctgaag
    10021 atattaattt attggcgatt aacacattaa aagaaatgtt cgggataccc gttgggtact
    10081 ccgatcattc attgggatat gatgttggta tttgtgctgt tgcgatgggt gctaaggtaa
    10141 ttgagaaaca ttttaccttg gataaggaac tacctggtcc agatcataaa gcttctttag
    10201 atcctcagga atttgcagat tttgtaaagc atattagaaa cactgaaata tttttaggtg
    10261 atggtgttaa gcgagcaatg ccttctgaac ttagtacaaa agaagtatca agaaaaagcc
    10321 tagtggtcac tgagaattta aatgtgggag atattcttac agagaaatct cttacaataa
    10381 aacgtcctgg aaatggaata gctcctaagt ttttatatca atatattggc aaaaaagtta
    10441 aaagaccaat aagtgcagat agtgttctgc ttgaggaaga tgttgaatga aaaaaatact
    10501 tatagtaagt ggaacacgag ctgattatgg aatatataaa cctgttgctg agaatttact
    10561 acaagataat tgggatgtat catttgttgt ttctggaatg catttatccc ataaatatgg
    10621 atttactaaa gaattgatct tagctgacgg atttagaatt agtggagaag ttcaaagttt
    10681 gtttttagag aacaataagg gaaacatggc tcggtctgtg gcattagaga taaatggttt
    10741 cacaaatata tttgaacaaa ctagccctga ttttgtatta ttactagggg atagaggaga
    10801 aatgttggca gctgcgatag cttgtttgta tcttggaatc aaaacagcac atattcacgg
    10861 aggcgaagtg tctggtactg tagatgaatc aataagacat gccataacga aaatagcatc
    10921 tgttcatttt gctgctacgg aaaaaagcaa agagcgttta ataaaaatgg gggaagattc
    10981 ttggagagtt tttcccgtcg gagctcctag aatagatact attttaaata atagattacc
    11041 tagttttaga gatataaaag aaaaatatca tctaaaattt gaaaagaaaa aatacgtttt
    11101 gcaagtattt catccagttg taaccgaact agaaaatata gaaaaacaaa ttttagcttt
    11161 gatcaattca atggaacaac aatctcaacc aattatttgc attttaccta atagtgatgc
    11221 tggtagcgaa attatcagaa aaatgtacca gaaatattta aaaacggata ttatttatat
    11281 tacgaattta gagccggagg aatacctgac tatattaaaa gagtgcaaat ttatgatcgg
    11341 aaattcttct tctgggataa ttgaagctgc atcattcaaa ataccagtaa taaatttagg
    11401 aaccagacaa aatggacgag aacaatctaa aaatattata aatgcaaatc taaatgaaaa
    11461 tgaaattgaa actgctttga ataaaataca agatcctcag tttataaaaa gcttgaaaaa
    11521 tgttaagaac atctacggtg acggcaacag ttctagaaga ataacaaaaa cattgaatca
    11581 aatattgaat gatggggaag atattaagtg gattcaaaaa caaatagctt attaaaatat
    11641 actagtgatc catacattat tattggggct ggcggacacg caaaagtcat tattgaaacc
    11701 ttaagattag aaaataagtt aatatatgga ttttttgatg ataagcaaaa taaaatgcaa
    11761 gatctgaaat atcttgggaa aataaacgaa ttgaatagct gggcggatag caaaaaaaaa
    11821 atctataaat ttattgtagc tattggtgac tgtgtagtaa gaaaaaagat tataaaaaaa
    11881 ttgaatatgc caagtgagca atatggaata gctgttcatc caagtgctat agtggcaaaa
    11941 gaaatttcaa ttggttatgg aactgttatt tttgcgaatg ctgtaattaa cccagatgct
    12001 gtaattggag agcatgctat aataaacaca ggtagcattg ttgagcatga ttgtagaatt
    12061 aataactatg ttcatttatc tccaggtgta tgcttagcgg gtggagtaca tgtaggtgta
    12121 ggaactcaaa taggaattgg ttctcaatgt atccagatga agaaaattgg atcttggtgt
    12181 atgataggcg cagggagcac tattgttaag gatattccct cacattcctt agcttatggc
    12241 aatccagcta aaattaagaa agaaggaatt aattttgaat tctagaattt tattatcaag
    12301 tcctcatatg ggaggaacag aagaaaaata tgttgctaag gcttttaaag ataattggat
    12361 agcgccatta ggtgaaaatg tggataaatt tgaagaaagt attacaaaat atactggttc
    12421 taagggcgga gcttctgcgt taagttcagg tacagctgcc atacatcttg ccttaagact
    12481 gcttaatgtt ggaaaaaacg atattgtctt tgctccttca tttactttta tagctacagt
    12541 aaatcctatt ttatatcaag gggctgtacc agtgttcatt gactcagaac ctaatacatg
    12601 gaatatgagc cctgttgcac tagagagcgc attagaagaa gctgaaagaa acaaccaatt
    12661 acctaaggca atcatcgtag tgaatttata tggtcaaatg gctgattatg aacgaatttt
    12721 agctattgca aaccaattta atgttccagt gatagaagat gcagcggaat cgttaggttc
    12781 tacttataag ggaatttcgt caggcttatt tgggacaatt gggatctatt catttaacgg
    12841 taataagatc atcacaacct caggtggagg agctctagtg tctagtgata aaaaatatgt
    12901 tgagcattca agatttttag caacacaagc aagagatcaa gcaccgtatt atcaacatag
    12961 cgaaataggt tataattacc gtctaagcaa tatttctgcc ggtatcggac gtggacaaat
    13021 ggaagtattg aatgatagag tagctaaacg aagagaaatt ttcaatcgat ataaggaagc
    13081 tttttctgga attgatagaa ttgaaatgat gccagaagtg gatggcatgg tgccaaatcg
    13141 ttggttgtct actttaacga tcaatgatta taattctatt gatcaagtag ttaacgtaat
    13201 aaataaacta aatgaaaaaa acattgaaac aagagctttg tggaaaccac tacatttgca
    13261 accagtattt caaggggtaa aaatgtatta ccatgataat aatcttagtg tgtcagaaaa
    13321 gttatttaat agcggcttat gcttaccttc tggaagtaat atgacagata gtgaacaaga
    13381 atttgttatt gatgagttta aaaaaatatt caagtaggtg tgatatttta tgaacatagg
    13441 aattattggt ttaggttcca tggggaaaag aagacttaga ctcattcaag aacactttaa
    13501 tgatatttta ctatgtggtg ttgatactaa tgaggcgaga cgaaaggaag ttgaaaagga
    13561 atttggaata ccaacttata atagtctaac agaggcaaaa aatttgtttg aaataaattc
    13621 agtttttgtt tgtacatccc ctataaccca tgaatttatt ataaaagagg ctttaaattt
    13681 agattgtaat atctttacgg aaatcaattt attaaatgaa tattatgatg aaataatcga
    13741 agtagctaaa aagaaagata gattacttta tctatcttct acatttttga agagaaaaga
    13801 aattcaatat attaagtcta aagtgacgca taataaaaaa atttcttatc aataccatgt
    13861 aggacagtat ttacccgact ggcatccttg ggaagattat cgcaattttt ttgtttcaaa
    13921 caaaaaaact aatgggtgta gagaaatatt cgctattgaa ttaccttgga ttatagatac
    13981 atttgggaaa gttaaatcgt ttactgtaat gacccagaaa ttatccaatc ttgatattga
    14041 ttattcggac agtttcactc ttattattga gcatgaaact ggtatagttg gaactttaag
    14101 tgtagatgtt gtttcaagag tagctaaaag aaatttaact ataattggtg aaaagatttt
    14161 aatagaatgg gaaggtaacc cggattctct attttcatgg aatgaagaaa cttctagtat
    14221 gacagatatt caattgtacg atgaggttat gcatgataat aattattcac atactattat
    14281 tgaagatgca tatttagagg agattaaaga atttttcacg cttttggaaa agaaaatgga
    14341 accccaatat acttttgaaa aagataagta tattattaac ttaattaacg acattgaggt
    14401 aaattaatga tgaaagtaat tttttttggg ttagggtcaa ttggaaagag acatctaaaa
    14461 aatctaataa gctatggaaa aaagaattct attaattttg atataacagc ttatagtagc
    14521 agtggaaaaa aagagtttga agaaataacg tatattcaaa atagagacga tttggataca
    14581 cattatgata tagcttttat tacaaatccg acttttaagc atctggaaac actaaatgta
    14641 attgttgaaa aagttgatta catattttta gaaaaacctg ctttttctga gagtattgat
    14701 cttactcaat ataattatga ttacaaaaat atttatgttg ctgctccact gaggtataaa
    14761 aatatcatgg aagttgttcg taatagtatt caaagtaaaa aagtatattc tgtaagagct
    14821 atttgttcta cttatcttcc tgattggaga aaaggtactg attatagaaa taattattct
    14881 gcctttaaat ctatgggggg aggagtagaa ttagactgta tacatgaatt ggattatatt
    14941 ataaacctat ttggtttccc taaatcagta aaaaaacagt ttggtaaata ttcgaatctt
    15001 gaaattaaca gtaatgattt agcaacatat atattgagat acgatgataa aattatagaa
    15061 attcatttag attattttgg aagagttcct acaagaaaat ttcaattgtt tactttagat
    15121 gaatgtatca atgttgactt attaaataat aaaataagtt ttgaagaaaa cattgttaaa
    15181 gaaatgcctg aatctgctaa tgatatgtat gagaaggagt tgaaatattt ctttgaaaac
    15241 gtagtaacag gcaaagaaaa ctggaatgat ttatatcatg caaacgaagt attgaaaata
    15301 gctgaggaga aataaatgga aattttattt actatttgtg gtagagctgg gtctaagggg
    15361 tttaaaaata aaaatttgaa atatttggca ggaaagcccc tagtccattt cgctttagca
    15421 atgatagata tctatcaaca aaaacatcct gaacataatt gcacgacttg tttgaatacg
    15481 gacagtaatg agttagtaga attgacgact aagttaaatc cgtctgtgaa aattgtagaa
    15541 cgtaaacctg aattagctac agatactgta gggaaaattg aagtgatacg tgattcattc
    15601 ttacaactag aaagagaaga taatatattt gatttaatta tagatttaga tttgacttca
    15661 cctttacgtg aagtttctaa tcttgaagaa ttaatagatg aacatctaaa aaatttagat
    15721 aaagacgtct acttttcagt tgtttctgca agacgtaatc cctattttaa tatggtaaaa
    15781 agaattgaaa atgaagttac tatagttaat aaatcaagat acacatctag gcaacagaca
    15841 cctgaagttt ttgaaatgaa tgcctcaatg tattctttta aacctgaatt tctaaaaaaa
    15901 agtaattata tttttgatgg gcaatgtggg ataataaaaa tgagagatta tctaatttta
    15961 gatattgata gtgaagaaga ttttaaatgg ttagaatttt tgtttcctaa atttttagaa
    16021 gattcttcgg agcttcaaga tatctataat tatttatatc ggaggtaaaa ggatgagctg
    16081 ttatcttatc accggtgggg ctggcttcat tggttctacc ttagccaatc atttaggaga
    16141 agaacatgtg gtaatcgtcg tcgatgactt atcaatggga aaagtagaaa atcttgatat
    16201 gacgaaaaat atcactttta ttgagggaga tgtcgctgag aaatccttga tggaagagat
    16261 catgaaagct aataaatttg attatatctt tcatttagca gcagttgcta gtgtggcaga
    16321 ttcagttgcc cgaccagtag aaacacatcg tgtgaatttt gaaagtgtat taatgatttt
    16381 agaattgatc cgcaagtatc aacctgattt aaaacggatc gtgtttagtt cttcagcggc
    16441 tgtttatggg gatgaaccga ctttaccaaa aaaagaagag tccgtgattc gcccattgac
    16501 gccttatgcg gttgataaat tcgctgcaga acaatatgtg ctagattatt gtcatttata
    16561 tgatgtaccc ggtagcgcag ttcgtttttt caatgtctat ggtccaaatc aaaatccgaa
    16621 ttctccatac agtggtgtga tttctattct cgtagatcgc tataagaaac aaatcgtagg
    16681 agaagcaact tcatttacat tatttggtga tggaagccag tctagagact ttgtttatat
    16741 agatgatgta atccaagcat tacttttagt tgcgaatgag gaagctgcac tggggaaaca
    16801 attcaatgtt ggaacgggta aagcaaccac acttttggaa ttgattcatg caatcaatga
    16861 aattctcggt gtagaattga cacttgaata caaagaagag cgttcgggtg atatccatga
    16921 ctctctagca gatattacta agatccagtc aattggctat caaccgaaat atggtatttt
    16981 aagtggaatg actaattatt tgaaaactga aatcaattaa aacatactaa gataagatta
    17041 gagggtaaat tcaggctctt tgtcaataag gactgatgag ttttgtaaaa tcaaatctgg
    17101 gatagaa
//
LOCUS       DAFEOM010000055        17004 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.057, whole genome shotgun sequence.
ACCESSION   DAFEOM010000055
VERSION     DAFEOM010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.057"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(<1..124)
                     /locus_tag="LP524_002002"
     CDS             complement(<1..124)
                     /locus_tag="LP524_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HBL2235031.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVF"
     gene            complement(228..539)
                     /locus_tag="LP524_002003"
     CDS             complement(228..539)
                     /locus_tag="LP524_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBL2235032.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(541..738)
                     /locus_tag="LP524_002004"
     CDS             complement(541..738)
                     /locus_tag="LP524_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HBL2235033.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1132..2859)
                     /locus_tag="LP524_002005"
     CDS             complement(1132..2859)
                     /locus_tag="LP524_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HBL2235034.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2852..4045)
                     /locus_tag="LP524_002006"
     CDS             complement(2852..4045)
                     /locus_tag="LP524_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL2235035.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4232..4876)
                     /locus_tag="LP524_002007"
     CDS             complement(4232..4876)
                     /locus_tag="LP524_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL2235036.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4970..5104)
                     /locus_tag="LP524_002008"
     CDS             complement(4970..5104)
                     /locus_tag="LP524_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBL2235037.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5106..7238)
                     /gene="feoB"
                     /locus_tag="LP524_002009"
     CDS             complement(5106..7238)
                     /gene="feoB"
                     /locus_tag="LP524_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBL2235038.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFFMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7235..7708)
                     /locus_tag="LP524_002010"
     CDS             complement(7235..7708)
                     /locus_tag="LP524_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBL2235039.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8109..8333
                     /gene="nrdH"
                     /locus_tag="LP524_002011"
     CDS             8109..8333
                     /gene="nrdH"
                     /locus_tag="LP524_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBL2235040.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8492..10651
                     /gene="nrdE"
                     /locus_tag="LP524_002012"
     CDS             8492..10651
                     /gene="nrdE"
                     /locus_tag="LP524_002012"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBL2235041.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10701..11666
                     /gene="nrdF"
                     /locus_tag="LP524_002013"
     CDS             10701..11666
                     /gene="nrdF"
                     /locus_tag="LP524_002013"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBL2235042.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11755..12894)
                     /locus_tag="LP524_002014"
     CDS             complement(11755..12894)
                     /locus_tag="LP524_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL2235043.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13203..13916)
                     /locus_tag="LP524_002015"
     CDS             complement(13203..13916)
                     /locus_tag="LP524_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBL2235044.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14170..14871)
                     /gene="nagB"
                     /locus_tag="LP524_002016"
     CDS             complement(14170..14871)
                     /gene="nagB"
                     /locus_tag="LP524_002016"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBL2235045.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15104..16636
                     /locus_tag="LP524_002017"
     CDS             15104..16636
                     /locus_tag="LP524_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL2235046.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16827..>17004)
                     /locus_tag="LP524_002018"
     CDS             complement(16827..>17004)
                     /locus_tag="LP524_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL2235047.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5279 a         3494 c         2909 g         5322 t
ORIGIN      
        1 aaaaaacaca ttccactaaa tttgatgccg gttttccttg aggttttgtt ccagcacctc
       61 ctttaatttt tttaagctct tgatctgaac attgtttagc atttagtcta tttttttctt
      121 ccattacgat tccccctaca aactataaat acttttatgt cactgattca atatacctat
      181 ttttctaatc aaaaaatttt gagacatatt taatattata agtatcatta aaattgagat
      241 ttatctccat aatctgctcg atataaacca tttattccag cagttcttcc aatttcactc
      301 aaatttctta ttaattcctc ttgatattca gtgaatctta ttttattagt ataagcagta
      361 aaatatagat aattcaccaa tctatttatt aaaggactgg gatctaattc atatttttgt
      421 ttttctaatt ttaaatatac cttttgtaaa acctcaagaa tatcagaata tttaggatct
      481 ttacttatag atatatcatt atataattca tgaacaattt gcttagcatt ttttttcatt
      541 ttagcacttc cctggaattg ctccacctaa aaaaccacct atagacattc ctgcaataca
      601 agtagttgcc ttggcccaat cgaccgtaca tttattttta gtgcaataca ctccatttcc
      661 ataatatttt ccactatgag tggtaccccc atagataagt tgtgtctctt taatagacaa
      721 aatttttaaa tgtttcataa tctaatcacc cctaaattaa tgtttttaga ctattattct
      781 aacattcatt aaatttcaaa aaacaattcc ttcttgaaac gtagcaaaat aattcctgaa
      841 taagaattta atcactttta agtatataaa ctatgtatat tctttaatat tttttgagaa
      901 tgcacttcgt acataataaa ctttaatcac attagtcttt catacctacg atcgatctct
      961 ttgactcgca cccataaatt cataatagtt tgtcggcgtc tttgaacgtt tcgaagatga
     1021 gctacgcatt ctcttttttt atagacaaaa aagatagaga cgaacatgga gcttccaaat
     1081 ttggatattt gatccaattt tggaagctcc atttatttcg tgtctgtctt tttatttttt
     1141 ccagctttcg actaattctt ggttctcttt gatccagtct tttgctgctt gctgtggatc
     1201 ttttccattg ttgatttcca acataacttt ttccatatct ttttctgacc aatggaaatt
     1261 gtctaatact ttgtaagctt ctggctggtc ttctttcaat ccttttcgaa ccatcgtatg
     1321 gatctgctct tcgcttccca ttccgttttc aggatcttct aagtatttca gatcatattt
     1381 ggcaaacatc caatgaggtg tccatcctgt aactacaatt ggttcgtgtt ttttgatcgc
     1441 ttgacctagt gcaacggtca ttgctcctga agaagatgtc tctactttcc agtctttcaa
     1501 gttgtcatat ttttggatcg tgttttcagc agccgtaact acaccagctc ctggctcgat
     1561 tcctatgatt ttcttacctg cctgatcggt taactcatcg atcgaattta catccatgta
     1621 ggctggcact gctaaaccaa cttttgctcc tgtcaaattg gcacctaggt cttcgacttg
     1681 atctttatat tgtgcatatt gtgaaccatg agttttaggt agccaagctg aaaccatggc
     1741 atctgactcg ccatttgaaa cagatttcca catgattgaa ttatctaatg gcgtcatcgt
     1801 tacatcatag cccatttgtt tcaatacttc accgactaca tttgttgaag caacttctgt
     1861 atcccattca acataagata agttgatacg ttttgtttct ttggcagatg aaaaactccc
     1921 tataccaatc gcacctaaga tcactactac tacagcacca atgatcagtc cttgacgttt
     1981 tttacttttc tgtgttgttg ctcctgcagc ttttttattg tttggctggt tcaaatgctg
     2041 tgtaaaacgg tcaatgatga ttgctaagat aactaatgca accccgttga caaatccatt
     2101 cccgacttgt gctctttgca atgctgataa tacgccgcgt cctaagccgg gtgcaccgat
     2161 catagaggca ataacgacca ttgaaagtga cagcattgtt gtttgattga tccctgccat
     2221 gatcgtactt ttagctaaag gcaattctag tttgaatagt ttttgacgac ttgtgctccc
     2281 gaatgaatca gaagcttcta ctaattcttt aggaacttgt cgaatcccta aattcgtaaa
     2341 acgaacggtt ggtggcagag caaagatgac tgaggcaaat acccctggaa ccatcccgat
     2401 accgaaaaat gcaactgctg gaatcaaata aacgaatcct ggcattgtct gcataaagtc
     2461 aaggatcggt ttgataatac tttgggcttt actgcttttt gccatcaaga tccctaatgg
     2521 taccccaatg atgatcgaga tcacactaga taaaagaaca agtgtgacag tattcatcaa
     2581 atcattccat agaccttggt tataaataaa ccatagaccg attaatgtga acaacgtaag
     2641 tcctggacgt ttcttagaaa taaagaaagc tgctaccgtc aataacaaga taaacaaagg
     2701 agctggaatc actaacagta aatttgtaat ccccgacatc actgcctgtc caatcgtttg
     2761 taaaaatgaa aataggccag aaaaagttgt cgtaaaccat tctgtgatat tttctaccca
     2821 gtttgccact ggtaattgat agttcaatag attattcatg ttcactcacc tcgcttgttt
     2881 ctgctaatgc ttcaatcaca ctacctcgga tgacgacacc ttttaatttt ccatcctcta
     2941 caactgcaag tggagtcggt gagtcataaa taatattgaa aatatcactt accaccatgt
     3001 ctttcccaat caccgtcaca ttttcatcta ggaagtcgat caatggacgt ttttgcttcc
     3061 tagcttctaa cgcttgatct gcagtgatga tccctttcag gtgtctcttt ctgtcaacag
     3121 ccatcagcat gctgacttct tcattacgca tccttgtcaa tgcaacgttt ggtccatcag
     3181 attcgatatt cgtcgtcaat gcaggtacca tgatgttttg agccgtaagc actttggaac
     3241 gatcgacttc ttccacaaat tcacgtacat aatcatttgc tggattggtc aatatttctt
     3301 cacctgttcc gatctgcatg atttcgccgt ctttcatcaa ggcgattctg tccccaatcc
     3361 gtaaagcttc attcaaatca tgggtaatga aaataatcgt tttttgaaca ttggcttgca
     3421 ggtccagtaa ttcgtcttgc atttctcgcc ggatcagcgg atcaagagcc gagaacgctt
     3481 cgtccatcag caagatctca ggatcatttg ctaaagctct ggctaggcca acccgttgtt
     3541 gcatcccacc agataactga ctaggatatt ggtctttaaa tgaaagcaaa ctagaatttt
     3601 ctaatgcttt ttctgctttt tcttgtcgtt cttctttagg tacaccacgg acttctaacc
     3661 cgtattctgt attttccaag atcgttcgat gtggaaacag accgaagttt tggaaaacca
     3721 tattgatttt atgacgacgg acttctcgca attcttcttt gcttaacttc gatacatctt
     3781 ctccatcaat atagatactg cccgaagttg gctcaatcag acgattcaat agacggatca
     3841 atgtggattt cccactcccg gaaagcccca tgatcacgaa gatttctcct tcttttacgt
     3901 caaaattcac gtcatacaca ccaaccgttg caccggtttt ctttaaaatc tccgttttcg
     3961 gctggtgatc attcatcata tctaaagcgg cttgtgtctt tttaccaaat atctttgtta
     4021 aatgttttac ttctactttt accaaacaaa ttctcctttt ttatgtaaat tcatttttct
     4081 ttctgaaaaa agtgaccaca ctatgttaac atcatgtagt cactttgtca aaaatgaaag
     4141 aaatttgctt gtccaaactg aaaaaaactt ggctaaatct ttgtaaataa aaggtttagc
     4201 caagttaata tttttttaat atttcacaca atcattctgc aagctgatgc tccgggtaaa
     4261 aaaagtttgt catacgctgt agtgtatatt catttccaat aaaatgaacg atgtcccctg
     4321 ccgtaaattt agcatagggg ccaggtgaaa caagcagttc ttcattatga gaaattgcga
     4381 taactgtggc gccagtcgcc tgccagatat tcaactcgct aattgaacgg tccagatgca
     4441 ttgctttttc tgataaagat aattcaaacg gaagtaaagg attcatctta tgtattcgct
     4501 tcgtttgatt gaccagctga tccaataatt gagaaaaatg attcagctca tcttgttgct
     4561 tctccacact tgccatgatt tctgcttttg tatcttggat ggattgtaca tcttgatatt
     4621 gcaataggaa atcttttgct ttttccttcg aagcaacaaa tgccccactt ccatgccgta
     4681 cttccatgat tccaagatct accagcacat tgatagcctt gcgggtagtc tcaggagaag
     4741 cattgtacgt actggcaagt gttgagcggg catggatctt ctcacccact ttatatcggt
     4801 tttcaacgat acgttcggct aaatcaacag caatctgctg gtagcgcggg cggacgattt
     4861 ttttctttcc agacattttc tttcctcttt ctttttttac tttccctcat cttactatgc
     4921 tctagcaaaa taagcaaaaa aggggagaat agcctcttgc aacgactgat catcgcttga
     4981 tctgttcctt ttttactggg caagctgttt gacagtcatc acaattctta ccggatttta
     5041 aacgagtata gacaattgcg cctgcacttc caaagattaa cacacttaat aagaatgtcg
     5101 ccatcctatt cctctccttc taatgacaat atagtataaa actggttctt ggattttggc
     5161 tgacggaaca aaacataaac gagtcctatc atcagtaaaa tagcaagata cgtccctaac
     5221 gctacctgtc caccctccaa taagtgtccc aactgataaa tcacaaaact aactaaataa
     5281 gctaaaccgc attgatagcc tatggcgatc caagtccatt tcttggcatt catttcccga
     5341 tggatagccc caattgccgc aaaacaaggc gcacaaagta aattgaaggc taacaatgaa
     5401 taagctgctg cgggagtata tgtgtgttgt agcacttgcc agatttctct gccattttcg
     5461 gatacttccg catgaccaaa caaaatacca aatgtgccaa taatattttc tttagcgacc
     5521 aatcctgtaa tcgctgcgac tgttccttgc cagtttcccc aaccaagcgg ctgaaaaaca
     5581 actgccagga ttcgcccgaa aaaggctaag atactttggt cttcagaaac aggttgagca
     5641 taaaaattat aagaagagct gaaccagatc acgatactag aaacaaagat aatcgttcct
     5701 gcttttttaa caaaagactt gctgcggtca taggtttgtc ttaggaccgc tgaccattga
     5761 ggaaaatgat atgcgggcaa ttccatgata aaaggagctg tatctccagc aaacaaacgt
     5821 gtttttttca agctgattcc agagaaaata attgcagtca taccaagaaa ataggcagaa
     5881 ggagctaccc aagattgatg aggaaaaaag gcccctgcta ctaaggcaat aatcggcaac
     5941 tttgcagaac aaggcatgaa ggttgtgacc atgatcgtca aacgacgatc ttgctcgttt
     6001 tcaatcgtgc gactggccat gacgcctggc actccacatc ctgtagcaat cagcatagga
     6061 atgaaggatt ttcctgaaag accgaatttc ctgaaaagtc gatccatgaa aaaagcgatc
     6121 ctcgccatat atccgcagtc ttccagaaag ctcaagcata aaaacaatat aatcaattgc
     6181 ggtagaaatc ctaacactgc tcccacgcct gcgatgatgc cgttcaagat cagctcctgc
     6241 atccatcccg ctacttgcca ttcagctaac aatctgccaa catgttctgg taaccatgta
     6301 ccaaacaatg tgtcattgat ccaatctgtc cccattgttc ctactgtttg aatagccaga
     6361 taatagatca gccacatcac aaatgcgaag atcggcaagg ctaaccaacg attcgttgca
     6421 atctgatcga ttttgtcact catcgataat tggaaagaat catttgacac aacacagagt
     6481 gtacatagtt gggtaataaa ttcataacgt tcattgacca gaatcgtctc actatcgtcg
     6541 tccaatagtt tctctgttaa ctggatcagt tgctcgatct ctttttgctg agaaactgct
     6601 atctcgtaat cctctcttac cttctgatct cgttcaaaat atttcattgc ataccagcgt
     6661 ttcttgccgc tttgttctcc tagtacatct tcaatttcat gtaaagctgc ttctaaacgt
     6721 ggatcatatg tacagggaac aggaatagaa ggccgctttt ttgcagactc ctgaatcttt
     6781 tcgattagtt tttcgattcc ttgctttttc aatacgctga ttggtaaaac tggtacacca
     6841 agaccatagg aaagcttttc tagattgatt ttttttcctt cttttttgat gacatccatc
     6901 atattcagac caatgatcat aggaatgtct aaatccaata actgcgtagt taaataaaga
     6961 tttcgttcta aattggtagc atcaataatg ttgatgatca gttcaggctg ttcctgaacc
     7021 aaataatctc ttgcaatcac ttcttctggt gtataaggag acaaagaata gatgccgggt
     7081 aggtcctgaa tatgaatgtt cggatttttt ttcaacgatc cttctttttt ttctactgtt
     7141 acgcccggcc aattccctac atattgattc gaaccagtca agagattgaa caagctagtt
     7201 ttcccgctgt ttggatttcc gactaacgcg atattcattc tttttcctcc ctttgtatca
     7261 agattagttc ggcctctgac tttcgtaacg tcagttcata ccctcgcact gtcagttcga
     7321 ttggatctcc cagaggggct agttttcgga taactatttt tacatttcgc gtcaatccca
     7381 tatccattaa ccgtcgcttc actgcgcctt gtccgtggat tgctacgacg atcccagtct
     7441 ctcctgcttt taattgatca agagaagtcc agctttcaga ggaagcagtc tttttattga
     7501 caaagatttg acctagtatt tcccgactta ctgccactcg atgacttttt accagtaaaa
     7561 tggcattgtt gcctgataaa ttgacgacga cgattttact tccggcaacg atcccgatat
     7621 tttgcaaatg gcgttttgtt ttttcttctg tttcgattcg ttcgacttga taaacgaaat
     7681 tctgctttgc ctgcgctaag gtctgcatat tgattcctct cttgtgaaat aaatcatgta
     7741 ttaaaatcat tcattatatg ttctaatttt cttacgtgtt acgcaaataa atgagaatca
     7801 ttctcacata gaatactctc ggtaattgag aatgtcaatt atacttgtga tattatgagc
     7861 gaatctttag tattgataga taaaaatgag aaaaaaatat ttcttcatag ttttccacaa
     7921 ttttttctct attaaatttg ctattttccc acacgatact tgttttcagc tcttttttca
     7981 aaaacacaaa acttagtatt ccacagtttc cacaacacaa tatgtagtgt ttgttgtttg
     8041 acttttttac taagagtttt atactatcta ttatagataa taagaatgtg ttagaggagg
     8101 cactcaccat gaacgtaaaa atcttttcta aaaataattg tatgcaatgt aagatggtca
     8161 aacggttttt aacagaaaat caaattgctt ttgaagaagt caatatcgat gaacagcctg
     8221 aagcagtaga ctggttgaaa gcacaaggat tccaaagtgt tcctgtcatt acttctgatg
     8281 cagaaacagt tatcggattc cgtcctgatc agttgcgtaa acttgccagc taacatgact
     8341 aaaagaatca aaacgtgata catggatttg aagggcttga gacagcacca gtttttgtcg
     8401 caaacttcca aatccttttt taaagcttaa aaacaatgca atgatttgca atcaagcatc
     8461 atcaaatacg aaatgaaggt tgggcgtacg tatgagtctt aaaaatttac aagatgtcag
     8521 ttatttcaag ctgaataatg aaatcaatcg gccagtcaat ggtcaaatcc ctttgaataa
     8581 ggataaagaa gctttagttg ccttttttga ggaaaatgtc aaaccaaata cgatgacttt
     8641 tccatctatt atggaaaaaa tcaattactt gatcgatcaa gaatatatag aagcagaatt
     8701 cattcaattg tattcaccat catttattga aaagctatat caatttttgg acgagcaaca
     8761 tttttctttc aaatctttta tggctgctta caaattttat tctcaatatg cgttgaaaac
     8821 caacgacggt tccgcttatc tggaaactta tgaagatcga gtagccttca atgcgcttta
     8881 ctttgcgaac ggggatgaag aattagcact tgctcttgca gacgaaatga tccatcaacg
     8941 atatcagcca gctacacctt ctttcttgaa tgctggacgt aaacgtcggg gcgaattggt
     9001 gtcttgtttc ttgactcaag tcactgacga tatgaactct atcgggcgct caatcaactc
     9061 tgctttgcaa ttgtcccgta ttggtggcgg cgtagggatt actttatcaa atcttcgtga
     9121 agccggcgct cctatcaaag gatatgaggg tgctgcaagt ggtgtcgttc cagtcatgaa
     9181 attattcgaa gatagtttca gctattctaa tcaattaggt cagcgtcaag gagctggcgt
     9241 ggtttatttg aacgttttcc atccagacat tattgctttc ctttcagcaa aaaaagaaaa
     9301 tgcagatgaa aagattcgcg taaaaactct ttctcttggc gtgatcgtac ctgataaatt
     9361 ttatgaattg acacgtaaaa acgaagatat gtatttattt agtccatata gtgtcgaaaa
     9421 agaatatggt gtcccattct cttatgtaaa tattacagct gagtatgatc atctagttgc
     9481 aaatccaaac attcgcaaac aaaaaatcaa agcgcgtgat cttgaaaatg aaatctcaaa
     9541 attacaacaa gaatctggct atccttacgt aatcaatatt gatacagcga accgcgagaa
     9601 tccaatcgat ggcaagatca tcatgagtaa tttatgttca gagattttac aagttcaaac
     9661 gccttctgtg attaacggca aacaggaata tgaagtatta ggaacagata tctcatgtaa
     9721 cttaggctcg acaaatatcg tgaatttgat gcaaagccct aactttggtc aatctgtgcg
     9781 tacgatgact cgtgcattga catttattac agatgcatct gatatcgatg tcgtaccacc
     9841 gatccaaaac ggaaataagc tgaatcatac gattggatta ggtgccatgg gcttacacac
     9901 ttatcttgca aaagaacaaa tggaatatgg ctcagaggat tcattagact ttacaaatat
     9961 ttacttcatg ctgttgaatt attggacact tgttgaaagc aacaatatcg ctcgtgaaag
    10021 aaaacaagtt ttccataatt ttgaaaaatc agcttatgct gacggcactt atttcgaaaa
    10081 atacgtaact ggagaattcc agccaaaatc agaaaaagtc aaagcattgt ttgaaggcat
    10141 ttttatccca actgctgaag attggaatgc tttgaaacaa gcagtaatga aagatggact
    10201 gtatcatcaa aatcgcttgg cagtagcacc aaatggctca atttcatata tcaacgatac
    10261 gagtgcaagt attcatccta tcactcgcat gatcgaagaa cgccaagaga agaaaatcgg
    10321 taaaatttat tacccagcgc cttatctatc caatgagacg attccgtatt acacttctgc
    10381 ttatgatatg gacatgcgta aagtaatcga tgtttatgca acggcacaac aacacgtcga
    10441 tcaaggcatg agtttgactt tattcatgcg ttctgaaatc ccagaaggca tttatgaatg
    10501 gaagacaaca acgaagcaaa cgacacgtga tttgaatatt ttaagacatt acgctttcca
    10561 caaaggaatc aaatccattt attacgtacg tacctttact gatgatgcag aagaaatcgg
    10621 aagcaatcaa tgtgaaagct gcgtgatcta atatcataga tagattcgct tattcatatt
    10681 ttaattaaag gagcattctc atgtcagaaa catattatga agcaataaac tggaatgaaa
    10741 ttgaagatat cattgataaa tccacttggg agaagctgac agagcaattt tggctagata
    10801 cacgtatccc actttcaaat gacctagatg attggcgtac attgtctcaa ttggaaaaag
    10861 atacagtagg acatgtattt ggcggtttga cactgctaga tactgtccaa tctgaaagtg
    10921 gcatggatca gttgcgtaaa gatgtccgca ctccccatga agaagccgtt ttgaacaata
    10981 ttcaattcat ggaatccgtt catgccaaaa gttattcttc tatttttagt acactgaata
    11041 cgaaaaaaga aatcgaagaa atttttgact ggacaaatac gaatccttat ctgcaaaaaa
    11101 aagctgagcg tatcaatgaa atctacaaac acggtacacc gctagaaaag aaaattgcca
    11161 gcgtcttttt agaaacattc ttgttctatt caggttttta tactcctctt tattatttag
    11221 gaaacaacaa attggctaac gtagcagaga tcatcaaact aatcatccga gatgaatctg
    11281 tacatggaac ctacatcggt tacaaattcc aattaggatt caacgagtta cctgaagcag
    11341 aacaagaaac gttaaaagac tggatgtaca atcttcttta tgagctttac gaaaacgaag
    11401 aacgttatac agaagaatta tacgatccaa tcggctggac agaagaagtc aagacattcc
    11461 tacgctataa tgccaacaaa gcattgatga acctagggat ggatccactc tttccagata
    11521 ctgccaatga tgtcaaccca atcgtaatga acgggatttc aacaggcaca agcaaccatg
    11581 acttcttctc acaagtcgga aacggctatt tgttaggtca tgtagaagcg atgaaagacg
    11641 acgattattt gattggattg gaataaggat ttattttaat tcaaaaattt ttcgaaaaat
    11701 aaaaagagtt tagcgtacag atattttaaa tgtacactga actctttttt atgcttattc
    11761 acttgtttct tccagcactt ttggactgtc taacttttcc gaaacatgtt ccattcttct
    11821 caataaatag attttccgga tatttaatat tatcgtctta atcactgcat agacaggtat
    11881 gcaaatcagc atgcctaata ttcctgcgac acttcctgct ccaatcaata ccaagatgat
    11941 cgtcaaagga tggatattca ttgatttccc aaataacaac ggcttgatca aatttccatc
    12001 taattgttgg atcactaaaa tcgacaacgc catatacaaa gcttgaaatg gagagataaa
    12061 caaaccgaca atgattgctg gtaccgctcc aataaaaggg ccgacatacg ggataatatt
    12121 cgtgattccg caaatgaccg caagcaacaa gctataaggc tgatcgaata ttctcattgc
    12181 caaaaagctc attacaccaa taatcaaagc atctaagatc gtactgctga tataggcaga
    12241 taatgtttcg tttaattctt ttaccgtttg gcgtaactct ttacgaatcg ctttcggaaa
    12301 gaagtgagac aacgcatcaa taaatttgtg cccatccttg aacataaata gcaaaataaa
    12361 tggaacggta aataacaaaa taaaaaattg catcataaat gaaatgattt ttgagacact
    12421 tgcagataaa ctgactaata tgatattgaa caaattacta agagataaat ttgctgatgc
    12481 taattgttct tccaaattga aatttttaaa ctcttcccgc tgcgccaatt catttaacca
    12541 tttgcttgtt tcatctgcat acattggcaa gctctgggtc aattgaattg tctgttccat
    12601 taactgaggc actacattca tgacagttaa aacaactaaa atgattacga ccaaaaacgt
    12661 caataaaaaa ccgaacactc tcgggatccg atgcttttcg agaaaaacaa cgattggatc
    12721 gaacatataa tagataaaac cagcaacaag caatggtaaa aaaattgccg aaagcatttt
    12781 caatattgga ctaaaaatat tcggcatttg taaaataaag tacacgccga tgatcgtgag
    12841 tactagctcc actgtccaga aaaataattt tgattgttta aaacgtgatt gcatacatct
    12901 cctccaaggt cctttgatac ttctattcta tcatttaaca aagagttacg cgctgagatt
    12961 ttcatttgct taaaaaaaac aaaaaaaata aacatatttt aatgagtaga cgagtactta
    13021 aacaaaaaca cggtttggag tagtgacaaa aattatactg cagtcccttt ttccaaataa
    13081 acggtgttca aaagcttgca tcttcgtaat aagcccagcc aaacgaaaaa tataaggaac
    13141 tatttttatt tggctgggct tattacttga tgtaggacat ctattttaca acctcagatc
    13201 atttattgcg caatactgac taaatgcaac tttgtgatat ttttgtgtac ggacaactcc
    13261 tcgatcacat cggcataagt gagccctttt ggaagatcaa tcgtatagat atttttgtac
    13321 actcggtagt catcaacgaa ttttacgtca aaattcacat cttcgattac gatatttttc
    13381 tcttcaaaat aatcggtaag aaaatctttg gtttcttttc tatggataaa ttggatttct
    13441 aactttttag tcgtaggtac atggatcacg cgtttcacta ctgtgagtgc aatagcaatc
    13501 ccgataaaac tgatgatcgc aatggaatag taacccatgc cgatggcgat ccctaatcca
    13561 gcaattgccc ataaagaagc ggcagtcgta agtcccgtca ccgattgttt cgtgacgatg
    13621 atcgttcccg cgcctaagaa accgacacca ctcaccactt gagcaatcag ccttgcttca
    13681 tctgaacgaa tcgttccagc aagctgtgga tactctaaag catctcttaa tgcaccacta
    13741 gcgatctcaa cttggatcaa tgcaatgata catgctccca tacaaactaa aatatgcgta
    13801 cgcatacctg ctggacgatt tttatattgt cgttcaaaac cgattgctcc gccaaatagc
    13861 atagcgatcc ctaaacgtaa aataatctct ggaattgtca ataccatatc attcatctaa
    13921 acaccctcca gtccaatata tcatgtctga aaggagcaga caaaaaaagt gcaattttta
    13981 cctcttacga aaaaaggact gtgacaaaat ttttgtcata gtccttttta tgaataaacg
    14041 gtgttcaatc agctgaccct cggtattact caacaaacgg caaaatatgg ggagctattt
    14101 ttcctttgtc ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tttagcctac
    14161 tattgaaaat tacaacttac ttgctgcttc ttcatcgatg atcacaacaa cattgtcgtg
    14221 attttgcaat acacttgctg gcaaatgatt cgttacaggt ccttcaatca tccctttgat
    14281 tgcatcagct ttgctttcgc caaatgccat caagacgatt tctttacttt tcataattga
    14341 accgattccc attgaaacag cttttgttgg tacatcttct gctttatcga agaatcgttt
    14401 gtttgcattg attgttgatt ctgttaagtc aactactgaa gtcgttccgt caaatgaagc
    14461 accaggttca ttgaaaccga tatgtccatt ttgcccgatt cctaagattt ggatatcgat
    14521 tggatgtgca tcgatgattt gatcatactt tttgcatgct gcttccagat cttctgcttt
    14581 tccatctggt acaaacgttt ctttgaatgg ttttttatca aataattgga catccatgaa
    14641 atgacggtag ctttgttcat ctgaaccgcc tagtccaacg tactcatcta agtttacaga
    14701 agtcatttca ctgaaatcca aatcgcttga agtcatttct ttatataaag taactggtgt
    14761 gcttcctgtt gccaatccta atacttttgc acctttgttc ataccttctt tgatgatttc
    14821 aaatgctttt tttccgcctt cttcagcgtt ttttacacga atgatttcca tagtgattgc
    14881 tcctttatat tttggtatag cccaattata gcattctttt tttcttttta caataattag
    14941 tatagaccaa attgcatttt ttcattttct tgacaataaa taggaatccg tgttaggttt
    15001 cataagatgg ttcaatcaaa caatccatgt tgcatgaaaa ccaagcaagt gattcactgt
    15061 ggacgcttta tgtctgcagt ttttttatga aaaaggagtt attatgagta tactgacatt
    15121 aaacaacatt acccagcaat ttggcgacaa aatcctctat gaaggtgtcg atctgcaatt
    15181 aaatgctggc gaacatcttg ggctgattgg acaaaacgga gccggtaaaa gtacattgat
    15241 taaaatcatc actggtgaga tcttaccaga tgacggacag atccattggc aaaaaaatat
    15301 tcacaaaggt tacctcgatc aatatgtgga agtcgatgaa acattaacaa tcgaagaatt
    15361 tctaaaaact gcgtacgcaa aagaatacga aaaggaagaa aaaattgctt ttctttacca
    15421 aacatatagc gagaccttcg atgattcctt gctggaaaaa gctgggaaac tccaaacgga
    15481 attagaccaa ggagtgtttt atcaaatcga caccttagtt gctgagatga gcagcgggtt
    15541 ggggatcgat gtcttaggtt tagattcact gttgaaaaat ctaagtggtg gacaacgttc
    15601 aaaggtgatt ttagcaaaac tacttttaga aaacccagat gttctgatct tagatgaacc
    15661 aaccaatcac ttagatgatc agcatatcca atggctgacg cagtttcttc aggattttga
    15721 cggtgcgtat atcgtgattt cccatgacca agaatttctt gatcagatca ccacccatat
    15781 cgcagacatc gagtttggaa agattactaa gtatactggc catctaaaac aagcattgaa
    15841 acaaaaggaa caaaatcgcg aaagctattt gcgccaatat catgctcaac aaaaacaaat
    15901 cgaaaagacc gaagcttata ttcgaaaata caaagccggt tcccgatcaa cgatggcaaa
    15961 aagccgacaa aaacaattag ataaaatcga acggctgacg cctccagctt cagcaagtaa
    16021 agctgtcttt gattttccat tctcgcctat tgtcacgaca ttagcagtgg aaacaactga
    16081 tttagtcatc ggatatgatc gaccgctact tgcacccatc aatttgacca tccgctttgg
    16141 tgaaaatatt gccattcgag gcttcaacgg gattggaaaa tctactttat taaaaacact
    16201 gatcggtgag attgaaaaaa taagtggcga tttccacttc ccagataaca caaaaatcaa
    16261 ttacttttca caagatctgt actgggaaaa tccattagaa acaccactac aatatttaag
    16321 cagtcaattt ccaaaagcga ctatcaaaga attacgtcgc caactagcta aagcagggtt
    16381 agttaatcaa ttagcaagtg aacctctcac tacattaagt ggtggggaac aaactaaggt
    16441 gaaacttgca cagttgacaa tgaaccaagg taatttactg attctcgatg aaccgacgaa
    16501 ccatatcgat caagaaacga aagaaagctt acaagacggg atacagaaat ttccaggaac
    16561 agtaatcatc gtctctcatg aacaagaatt ttatcaagat cttgtgcaac gagtcattga
    16621 gattgaacca aaataaacgt ttgattgaat agttgaaaaa aaggagctgt gacaaaagtt
    16681 ttgtcacaac tccttcttct gaataaatgg tggccaatta gctgaccttc ggtattagct
    16741 caaaaaacgg caaaatatga ggagctattt ttatttggct attcttatta cttgatgcag
    16801 aacagctttt cacaacctct tttcgtctat tttttatttt cgagatttct ctttccttta
    16861 aaaacaactg tttgaaagaa gaacgcacat ccttgaaaaa caagcccgct aaccgttaca
    16921 cctaaccagc catactgctg ccacattgtc gtccctagca atgaaccagc cgcacctcca
    16981 ataaagtaca taaacataaa taca
//
LOCUS       DAFEOM010000056        16903 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.058, whole genome shotgun sequence.
ACCESSION   DAFEOM010000056
VERSION     DAFEOM010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.058"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(<1..189)
                     /locus_tag="LP524_002019"
     CDS             complement(<1..189)
                     /locus_tag="LP524_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235048.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGC"
     gene            complement(199..1014)
                     /locus_tag="LP524_002020"
     CDS             complement(199..1014)
                     /locus_tag="LP524_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL2235049.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1276..1719)
                     /locus_tag="LP524_002021"
     CDS             complement(1276..1719)
                     /locus_tag="LP524_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HBL2235050.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(1853..2191)
                     /locus_tag="LP524_002022"
     CDS             complement(1853..2191)
                     /locus_tag="LP524_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL2235051.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2179..2556)
                     /locus_tag="LP524_002023"
     CDS             complement(2179..2556)
                     /locus_tag="LP524_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL2235052.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2780..3973
                     /locus_tag="LP524_002024"
     CDS             2780..3973
                     /locus_tag="LP524_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL2235053.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4139..4561)
                     /locus_tag="LP524_002025"
     CDS             complement(4139..4561)
                     /locus_tag="LP524_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2235054.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            4956..5150
                     /locus_tag="LP524_002026"
     CDS             4956..5150
                     /locus_tag="LP524_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235055.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            5147..5641
                     /locus_tag="LP524_002027"
     CDS             5147..5641
                     /locus_tag="LP524_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235056.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(5803..6975)
                     /locus_tag="LP524_002028"
     CDS             complement(5803..6975)
                     /locus_tag="LP524_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL2235057.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(7181..7996)
                     /locus_tag="LP524_002029"
     CDS             complement(7181..7996)
                     /locus_tag="LP524_002029"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBL2235058.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNMI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(8146..8592)
                     /locus_tag="LP524_002030"
     CDS             complement(8146..8592)
                     /locus_tag="LP524_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HBL2235059.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(8589..9593)
                     /locus_tag="LP524_002031"
     CDS             complement(8589..9593)
                     /locus_tag="LP524_002031"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBL2235060.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(9633..9962)
                     /locus_tag="LP524_002032"
     CDS             complement(9633..9962)
                     /locus_tag="LP524_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HBL2235061.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(10088..12415)
                     /locus_tag="LP524_002033"
     CDS             complement(10088..12415)
                     /locus_tag="LP524_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL2235062.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            complement(13258..13551)
                     /locus_tag="LP524_002034"
     CDS             complement(13258..13551)
                     /locus_tag="LP524_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBL2235063.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(13954..15132)
                     /locus_tag="LP524_002035"
     CDS             complement(13954..15132)
                     /locus_tag="LP524_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL2235064.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(15236..15550)
                     /locus_tag="LP524_002036"
     CDS             complement(15236..15550)
                     /locus_tag="LP524_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2235065.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            15657..15872
                     /locus_tag="LP524_002037"
     CDS             15657..15872
                     /locus_tag="LP524_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235066.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(16172..16414)
                     /locus_tag="LP524_002038"
     CDS             complement(16172..16414)
                     /locus_tag="LP524_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2235067.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(16401..16829)
                     /locus_tag="LP524_002039"
     CDS             complement(16401..16829)
                     /locus_tag="LP524_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL2235068.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFLI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         4927 a         3271 c         2936 g         5769 t
ORIGIN      
        1 gcaacctgga ccaataaaaa gtaaagaaaa aataaaggaa aagacattcc ttccatctat
       61 cgacaaaagg aacgcagctg ctatatacaa gatattgaca caccaaaaac aaaggctact
      121 tgccaatgat tgatacaacc aataaaagaa ttccatataa acatttttgt caaaacgctg
      181 ctctttcata atcacctcct attccttcat tcctgatgaa gcgatccctt ctacaaaata
      241 tttttgcatc gctaaaaata taaaagcaac aggaagaaca gaaacaactg aggcagccat
      301 gattaatgcg tactctgcat caaatagccc aataaacatt ttcaacccta actggattgt
      361 tttattttcc gtagaagaca aataaatgaa tggccccata taatcgttcc atgtattaac
      421 gaatgtgatg atcgtcaaac tggcaatcgc cggttttgta agaggtaaaa tgatcttgcg
      481 atagattccc cactctgata agccatcgat ccgcgcactc tcacataaag aatctgggat
      541 actactgtag tattgcttca acaagaacac accaaaagca ttgaacgctt gtaatagcac
      601 caacgaccac aacgtatatg ttaaacccac ttttctcatc ataatgaact gcgggatcat
      661 ataagactgc caagggacag caatcgtgcc gatatatgct aaaaataaaa tatcgcgccc
      721 tttaaaattt agtttagaaa acccgtaagc agcaaaacta gaagtaaaca actgaatcaa
      781 cgtgatcaca acgcttaaaa aagctgtatt cttaaaaaat gtcactaaag gaatacgaga
      841 ccaaataacc agataattct ctaaatgcca agttttaggg atccattgaa tcggaatcgt
      901 gaacacttca ttatttgctt tgaatgaagc agaaaccatc cataaaaaag gaatgatcgt
      961 caataaagct aatacaatca gcagaatcat acccaaccat tgaataaagg gcatgttttt
     1021 tgaacgaatt tttgcttttt ttctttcatt tcgaacacct ccaaagttat aatcttcatt
     1081 tctttgtaat gattacaatc cagaacaacg gttattatag ctgattcatt tccgttcttc
     1141 ttatgtttgt aagcgcttat ttaacaagtt atgtaaatct ctttattttt tttgatttaa
     1201 aaatgaaaaa atacaaaagc aagactgtca tacgtctcgc ttttgtattg aaatttcttc
     1261 gattagagat acttattact gttcaaaagc ttctttgaaa cggtgaatca attcattgac
     1321 tacaggtgat aaaatccgat tctttttcca gactagcatg cagttcgttt caatcattgg
     1381 gtcaagcgga acgaatgtca ctcgctttga aagatgactt acagcacctt ctatgactaa
     1441 agcagcacct acctgattct caattagcgc aaatacattg aaaatcaaat tgtacgttcc
     1501 tgtgatcagc aactgctcct tttttatgcc taaccagtca gcaaacattc gctgtatttc
     1561 tggacgcttt gagatcaaaa gaggtacatt cgcaaagtct tcttttgtta tcacgtcttt
     1621 ttttgctaaa acagaatttt ctaaaatgag caatccccat ttttcctttc ttggtaacaa
     1681 aatcgtttcg tatttctctg tatctactgg atctagcaaa atcgctaaat ctaacaggct
     1741 tttttctagt cactccgtga tatcatcact tgttccgctg tggatatgaa attgaacgct
     1801 gggataatca cggatcaact tttctagtaa tagtgccaat gtattcgaat tatcagcttc
     1861 cacacagccg atcgtaaatc ggccgctgaa taattgtttt tttctgttct cgaattcttg
     1921 agtggtttga ttcgttaaat ctaaaatttc ttctgctcgg cttttttaga aaataacctg
     1981 cttctgtcaa gatcattttg cggttttcgc gtacaaacag ttctgttcct agttcctctt
     2041 ctaactcctt cagttgtcgg cttaaggttg gttgtgtaag atgtagaact tctgcggctt
     2101 gagagattgt tccttcttct gcaattgtcc aaaaatattt tagtaagcgt aactccatag
     2161 cgatgctcct tgattttttt acttgataca ttccatttta tttctagata acacacgatc
     2221 atatttcttg atttttcctt gtaaacgttc caaacactct tgcatttcag caatttcttt
     2281 cgtcaaaagt tcactttctt caatcaaaag cttacgccgc tcaaatacag ttgcttctcc
     2341 ttcgctagat aggcgaatat attcaatcaa agactcgatc gataatccgg catttcgcat
     2401 acagatcgta aagtcgaccc attttaggtc ttcttctgta taatcacgga ttccgccttt
     2461 tgtccgatta actggtggaa tcaacccgat tcgttcataa tagcgcaatg tatcggccga
     2521 aatgcccttt tcttcactga cttgtttgat attcatctga tcactctttc tcttaatttc
     2581 ttgccacttt acttatgtat tttatttatt ctacaacttt aagtgtactc caagtcaata
     2641 ctcatttcta ttaaaactaa gctggattat ctatatcggt tgtttgtgta ttatttcaaa
     2701 taaaaaagtc ttgttttttt attctttttt ggatatactt ttaacaattt agggaaattt
     2761 taggaaaagg ggtataaaaa tgacaacgta tcagcgaaag gtattattat cgacatcggc
     2821 gggaattgct ttagagaata tggatattat gtttttagct ttttcattat cttcaatgat
     2881 tacttctttt catattagtg gtacgcaagc gggtttcatt tcaacgatca ctaatttggg
     2941 gatgctcatt ggaggtattt tttttggcat catagctgat aaatatggac gtgtgaaagt
     3001 attttctcaa acggtccttc tgttttcgat tgcttcttta ttgatgtatt ttgcttcaaa
     3061 catctacttg gtttatctct ttcgctttat cgcaggtgta ggagcgggag gagaatacgg
     3121 ggcgtgtatg tccttgattt ccgaaagttt ttcaaaaaaa caaattggac gagcttcttc
     3181 ggttgctggt attggtgcgc aggtcggtgc ggctttggct gcgattttag ctgctgtgat
     3241 cattccaacg tttggttgga agatgttgta tgtcgtaggt gtactgccgg tgttgatggt
     3301 cttgttcatt cgtcgcggct tgactgaacc aaaggaattc aaatctgtac aatctgccga
     3361 aaagaaaacc aaacttagca gactgtttga atcaaaacat cttgcttggc aaacatgtgg
     3421 ccttagtatc atggtaactg tccaaattgc cggctatttt gggttaatga attggctgcc
     3481 ttcaattatg caaaatcgat tagggctttc ggtttctggg tcttcattgt ggatggtcag
     3541 tactatttta ggcatgtctt tggggatgat gacttttggc ataatcatgg ataaattggg
     3601 gcctaaacta gccttttctt tgtttttaat ttgttcatcc ttatctgtct atctacttgt
     3661 gttagcccat agccagtgga gtttggtagc agccgctgtg atagtcggct actttatcaa
     3721 tggcatgtat ggtggatatg gcgcagtgat tagtagtttg tatccaacag aaatccgcgc
     3781 gacggcgaat aatttcatta tgaaccttgg acgagctgtt ggtggttttt cttctatcgt
     3841 catcggtttc ttgatggatc attatagttt aactgccgta atcttgtttt tgagcggtat
     3901 ttacctagtc agtctgctgg tcttgacaac tcttactggt gttacagcat taagggaaag
     3961 cttgcagcat tgatagttga gtctgtaaat gacaagaaag aggtgtggca aaagcttgca
     4021 tctgcggtaa taagaccagc caaatcaaaa acatgagaca ctgtttccgt ttggctggtc
     4081 gtattacttg atgcagggca gctttttcaa ctgctaattt ccttctatct ggcggcgact
     4141 attctttgac gtccgtcatt tcaaagtact ttactaataa cttagcataa cgaaaaactg
     4201 tggatacact cgtatattga gcgtctgcat aatcttccaa atttttctca ggatgttgat
     4261 acatgaattc aatcaaatga taattatatg attttctgta gaaatgaaga taaataatgt
     4321 agattgggaa gttctcctgc ttttccatag aaaagcgccg attattctca ttcttcaaaa
     4381 tgaccttatc aggatatttt tggaacaatt cttctaaatc tgttagataa ttcaagatag
     4441 acttacttga taccttcaat tcgttttgta gttcttctat tgaacaccaa cgttctgttt
     4501 caagcagtaa atgaatcatt ttaattttct tttgagaaac ttttgttaaa aaagtctgca
     4561 tattttctcc tcctgtatct actttactat actacataca agaaaaaaaa cggatacaaa
     4621 tatcatattg tcacaccgtc aatagtgcat aaacctgtta ttatttctct ttttttccta
     4681 agtattctat tgatatgaat catttaatcg tttattatat aaatgtcaat aatctaataa
     4741 taaaataacg tacatatttt attattgcat acttaataac agaactatag caaaaaatcc
     4801 cgtgtatcaa cacgggatta gattaagttt cctcaatcac tattcataac acaattatta
     4861 taacaagtaa gtaaacaatt gaaaaggaca tttattgaaa acggttgttt tttacttaag
     4921 gaggaaattt agcgaaaaaa ttagttgcat tgtttttggt cgcaggaagt ttgttgagtg
     4981 ggtgtggtgc tactacgtct actactcaaa gcacaagttc agatcaggct tccagcagca
     5041 gtaccacgat ctccgaaagt acggcagatt catcgaaaga acaagaacaa attgaaaaga
     5101 aaaaagaaga gaaacaaaaa gcggaaaaag ctgcactaga aaaaaagtga cagaagcgga
     5161 atctgctttg tcacaagctg aagcaaatcc tacaaaagaa aactatgcaa ctgcttcagc
     5221 agctatccaa gcgctacctg aaaacaaaca agagttgaca agccgtcttg ctgcaattga
     5281 ttccacaatc aaagcaaaag aagcagaaga agaacggaaa aagcaagaag aaattgcacg
     5341 acaacaagca gaacaagcgc aacaagaagc aatcgcacag caacaagcag aacaggcgag
     5401 gcaagaagca atcgcacagc aacaagcaga acaggcgcga cagcaatcag cagctgaaca
     5461 ggcctctcag caggcacaag ccgcagctcc tgagcaaaat aatgaacaaa ctgtctatgt
     5521 cacaccgacc ggttcaaaat accatacaca taaatgtgga aatggtacgt atacccctgc
     5581 cactttaagc gaagctcaag ctcgtggttt aaccccttgc tccaaatgtt atggaaacta
     5641 attcaaaagt tatactataa taataaaatt cttctgaaac ataaaaaaat agaagatggc
     5701 gttccagata gtaaaccaaa aataacagtt tacacgtcca tttcgtcagc ttctctcttt
     5761 tttcatttag tctgttccct ctgctttttt ggacatcttc attcattctt tctcaatcaa
     5821 atagtacacc tctttttctt ttcgcagttt atatcttcct tttttcaatt tcattgaaaa
     5881 ctgtttcgaa tgtttatgtt ttacatggat tttaagcagg agctcctcat gttgcaagcg
     5941 atagttcctg ccttttaaat cagaaaatct cactagtccg tattcatcag aagctttcac
     6001 aaatggaact ttttctctag taatatagtg tttgccgttg agcgttttta cagaaaagac
     6061 cattccttcg attggtcgtc cgtttgtgtt ctttacataa aaagataaag gatagtttcg
     6121 cttagcgatg gccaaagaat ctaaataagc gatgagtagc caaactaagc cgattgatcc
     6181 aagtagtata ccgataaacc aaataaaaaa tttccatttt ttccattgat caattccttg
     6241 gatcgctttt tttgcctctt ttgcttgata aggaatacga tgccccgtca caagcagtcg
     6301 atgtgtattg accatataag gagtacaggt aagcaaagtc acgagatctc tgcctttttg
     6361 gatacccaaa gaatctactt catcaggtaa gaccacttgg attttttcta cttgatacgc
     6421 caatgtcttt ccattgatct ggatgaaaaa ctggtctcct tttttcaact taggcaaatc
     6481 tgtaaataat tttgcttctg ggagtccacg atgcccagaa agaacggcat gggtgttttt
     6541 cccgccagtc ggataagaac tttcttccaa taatgacgtt cctttttgca aaaatatttc
     6601 cgttgtctga tcgaatatag gtaagctgac attgattttt gggatttcga tgactccaag
     6661 catatgttcc ataaaaaagg cattactagg taaatctttt aatgcttttg catcaactgc
     6721 ttggttgaaa ctgcctatcc ctggtgatac gttttgctct gccaactgtt ggtttttctt
     6781 tgtcattttt tcctgctgtt tttttatttc ctctgaattc ttattcgatg cttgcttttg
     6841 ataatggatg atcatttgct gggcaagaaa atcattcagt gaatcttcta caaaaggata
     6901 agaaaagatc ccgatcccaa cgactaaaat gattgccatg aagatgtcca gtattcttat
     6961 tctgctttta ttcatcatgt ccacctattt tctaatatgt atatgaagag gttgtgaaat
     7021 cagcgccttg acctgagtat tagcgaagca aacggcaaaa tagttcctcc tattttgccg
     7081 tttgttgagc gaataccaag agaccgacca cttgtcacaa cctttctccc catttgaatc
     7141 actatcgttt atttatcgta tgatttttgt ttccaatagt ttaagcttct gtttcttgat
     7201 ggcgtttttt gaaatagatc actgctcctc caactaaagc agtacctacc aagatgaata
     7261 cgatgatccc cataccacca gttgaaggca agattccttt aggttggtta tcgatcgttg
     7321 tttcatcaga agttgttgga tcatcatcta ctgtaaattc agtgtacgca gcttcattct
     7381 taggtaatgc gtagccttct ggtgcttttg tttcttgaag gcagtatgcc ccttgtttca
     7441 gcccttcgat tgaaaatttc ccttctgaat cagaaatcaa tttcacttct tcccagtcag
     7501 aacctgtgac ttcatctgac caagcatatg ggtattctcc tgaggcaagg tttgctattt
     7561 ttttcattaa tttaacagtt ggatcttttg ctcttagaat agaaaattct gcatcggcta
     7621 atttttcttg cgtgttcaca tcttgtttga tgaagtggaa atcaccgtag ttttggacat
     7681 tttctacagt aatcatgtta tccgtagttg tattttcccg attgactaca ccgtttggat
     7741 tcccaagatt ggcttcatct gcgccatgga tgataccggt tgtttgttgc aattttccgt
     7801 tttttactat aaaagtagtt tctttttgtg gattggttaa ttgataacca tctggtgctt
     7861 ttgtttcaac taaagtataa ttgccgtcta ttaatccatt gacactgatg gatccttcag
     7921 tatcagttgt gaatgtcaac gctgactcag caggatcata gagagcgaca ccagtgtttt
     7981 catcaaaacc ggtcacgaat tgattctctg aatttttgat ctggaattgc gcccctgcta
     8041 acttatctac ccctgaaaag ttcccagctt tctggatttt gatattgctt tctttgatga
     8101 catttttagc atataggtga atcgtgtcta aaggtttgtc agggtctagg gtaagcttaa
     8161 cacgatattt ggcgacgttt cgacaccggg ctttggtgtt tctacaaaga cgtatacagc
     8221 atctttccca tcagatttac ctggaagagt caggttagct aatccttcac cagaagtcgt
     8281 tacacttgcg aagggtgtag cgtctgctgg aactgctgtg tctttacttg gatagtacgt
     8341 agcagtgatg tcatatacgt tgaattcaac gccgttcaac ggttcgctat tcggaaatgt
     8401 caattccgtt ccgttgttta tttgattttc tggtgctgaa gtgaatttca atttatgcaa
     8461 attaaccgta acattttctg gtgtaggtga tacggctgca gaaacctctg cagttcctag
     8521 gccgagaacg aaaggaagca gcatgatagc agttcctaaa aacgtccata tttttgtttt
     8581 gatgtttttc attttatttc cctcctattg tttccttttt gttgtttttt ataaaaccaa
     8641 gctgtccctg ttgaaaggac tagcaataac cctaaaattg agaaacctcc aagcatctct
     8701 tcgcttgttt ttggaaagct tcctccattt ttttcatttg atggaccatt tgtctgcatc
     8761 tgaactggcg tttttccatt attcgtcgga tttgcttttg gttcgttgat gatttccaat
     8821 ggtataggat gaagcggatc ataactgcct ttttcaactt caaagagaat ggttgattga
     8881 ctcaatatat atcctttagg cggtttaatc tccactagtt cgtaacttcc aaaagacaat
     8941 ccatggacag aaaatgttcc atctttactg gaagtaaaaa tcgttgctct tgaatcatct
     9001 ttggctaccc aatgatttat cccgttttct tgcgctaagt attggtcatt ttttttgact
     9061 agaaattgag catctgctag tttttgtttc gtttgccgat ccactttgac aaattgtttg
     9121 cctcctgttt ggatggcaag atgtgtttct actgtttgat tgtcagtcgt caaacgtaca
     9181 tcgttatcaa atgtcgtttt ttcactgttt tctttcaatg tcatttggta acggatcgtt
     9241 aatttttttc cagcaaacga actaagatct tttccagaat ctgataagac aaaaccatgt
     9301 tttatttttt gtgtgtgaaa tgtatgaatc ttgtctccac caatcaaaaa atcgatcgtt
     9361 tctggttcta gccataactg aggatcagct gtatctttga tcgtataggt tcctatctcg
     9421 ttgatgttct caggaattgt tacggtgatg agataaggga ctttatcccc atctgcaaaa
     9481 tctgtctgct tattctctac tgtttttgtt aatgtgagtg cagcaggtat tttttgttcg
     9541 ctctttggat acaagtggat ggtcgtcaat ttatgattag atgaatctgt cacaggaagc
     9601 acaacgacaa atggtgctga tttttctttt acttaatctg aagtctttat ttccgtaaat
     9661 cggtaaacag cgtcacgacc ttgctcatct ttgtccgatg cactaaaaga agcaatgccc
     9721 tcttcctcgt ctactgtttt tgtcacattt tctgccagta ttttttcact atcacttttt
     9781 tgtgctagtt ttctttgtgc gtcttccaca ctacttcctt ctgaccgcag cttgtaaaat
     9841 tcatccgaga catcatatat agcgaaagta acgccgttca acccttttac tccttgaaag
     9901 ttatccggga tatttattcc gtcgttttga ctgatacttg gcatcttatc gctttcgaac
     9961 attcgattat gcaaagtaaa tgtaataggt gattctctag ctgacacagt cggagaacct
    10021 ataaaaaaga ccagtaggat aaaaaagaga acagcaaata tatccacacg tacatttttt
    10081 gtttttttca tcgctgttca cctgcttttt tcttacgatg gataaaataa aagaagccag
    10141 aaaaaataaa gatcagtcct aaaaagacaa agggcagtag acctatccca cctgtcgaag
    10201 gaagcaagag agtcggattg atcgtcaact tagcggtatc tgttgcgata atcgtataat
    10261 tattgtttac acttccttcg cctaaactaa tcgttactat ttgtgatcca atatctgtcc
    10321 ggctgttaac tgttagggta taatccaatg attcgcctaa gacaacaccg gtaacgtgga
    10381 gatttccaac cgaaatcgag tgactttggg catcataatt aaaggcagcg tcatcgacag
    10441 taatcactgc gttagcttta tagatggtat aagttcctga tgtgttggct atatcagtaa
    10501 atgacaactt gggatttgct gctttgactt tgtctattcc gctattcgtt agtttaattg
    10561 tatagctgcc agcattaacc atattttttg ctgattgtgc atctgctgtg taatccatac
    10621 tgctaagtgc cacttgtact ggagtaagcg aactagtgac accctctgct agcttgacat
    10681 ggattgacgg gacacctgct tttccatcat atgtcttact gccactgagc tctaccgcac
    10741 cgctgatttg cttaatcgta tagacatcag atgtctgcac gatagtaaat aaatagtttg
    10801 ttctggcagc tagtttagct agtccgcttt ttgtcaattg ccaagacgta taggttccaa
    10861 tttcagtagg cgcagttact tcaactccct tttcattcat cgctttgaaa tcagttggat
    10921 tccatgtgat ttgatcaata ctgagatcat tgtttaaaag ggatgttgat tctgttttat
    10981 cattaaaggc atatttaacg atcggtacaa cagaaagacc atcatagtac ttactgccat
    11041 ttgcattgat tgatactgtt gcatttgtct tataatatag gatgacttca gaaatctgtt
    11101 ttccatttgc gtcctttacg attacatttc catttgtatc taaactagct aaaacactca
    11161 ttgctgattg gttctttgtt tctatcgctg aaacagctgt aaaggtatat ccgttgatag
    11221 taggcgtatt agtcgtcagg tcttgtgcag cacttccgta agtaagattc attattttag
    11281 aaggagcaat catctgtcca aaatagtctt gatacttaat agtgattggt gtttgtttgc
    11341 ctgtataggt caccgtatcg acgttacttt ctagggctgt gaaagaaccg tttgtttgtg
    11401 caaaagaata gcccgttact gctgttggag tgataaccac tttgtcaccc acatggattc
    11461 cagataatgt agtcccgtac gttccgattt ttgctgctcc accattgact gtataagaaa
    11521 aagttcctgt ccgattcgca ttgacctgaa ccgttgctgc ttgataggtt ggaaaatttg
    11581 aaacatttcc cttaccatca acgatctgcc catttttgat cgagtacccc gtgaatggaa
    11641 tatcatttgc taactgtata ccgattgaaa aagtactcat acctcctccg gcaggaagcc
    11701 ctccccatgt tgagggtgta aaagaataac ttttatccgc attgggtgta actgaatcga
    11761 tcgtatttcc agcggtagcc acccccatat agatactttt tgggaatgtg atagtagtac
    11821 catttactgt ggagccaata aacagattat ggatatctgg acggttatca ataaaagcga
    11881 ttgctttggt tacgccttgt aacgcagttt tatccgcact catcgatccg ttaaccgcaa
    11941 caatgactcc atctttggca tatatcactg tatatccatc tggtatggta tgtccgttga
    12001 aattcgcagt ggccgcaaca gtactaaatg tctggcttct acctgtttct gaataagctt
    12061 tctcccctgc tgcttttgct gctgtgattt ctttttctgt cggatcaatc agttgagtcg
    12121 aggatgtggt tgaggttgtc tcgttgttgc tagactgatt ggtgctttct atggactgta
    12181 tttcagaata gggtggacta tttgtctcct ctgtcgtttg gttagcaaaa tttgacgttt
    12241 ctgtataaac gtttacataa atacttgata agctccctaa gaaagtaaaa atcaagatga
    12301 tcgaaatcat aatgggatgt aagtattttc ttttagatac agctttgagt ctagaaacaa
    12361 aatgtttttg acatatacgt tggattttat tttgagtttt cttaatcatt ttcatccctc
    12421 ctctcaataa aaaatcatca aataatcctt tacacgttat tctaaattta atttttatta
    12481 ttttattgat gtatagaaag tttggggctt tttttctctt tttattctaa ggttatttta
    12541 gtcttgtcct ttaatacaaa gattttattt ttaaataaaa aatactcttt ttttacgatt
    12601 attgaaaaaa aaagataaaa cttgattaat tgaagtattg cttaaatttg gaagctatga
    12661 gggaagaata aaagagttag gacgggatta tctactattt ttctttgttt taaatatttg
    12721 ttatctttga acataaaaga gaaatgtgtc gactgcagtg ttgtgtccct catcacgaat
    12781 aaacggtgtt caattagctg atcttgtatt cacttaaaaa atggtaaagt acgagttgct
    12841 attccccatt ttcaattttc taacgctcag gacaaaatac tgttttcaca acctcattgg
    12901 aataaaagca tatatattgt ttcaagaacg aaatcttgtt atctttttaa agggcatctg
    12961 tccaaaatct aattttagga ggtacgtttt ggtatttatt cgtacaaata aaagagaatt
    13021 atctttattc atgtaacgca tccaattgat tttttcatgt acgctcctgg aacgatttta
    13081 ctgacgattg cttatttaac tgcgaatcgc tcattagctt ttatcatagc tatccatatt
    13141 ttgaataacg ttcttggctt tgtattatag atacagatga caaaaaaagt tcaagtatgc
    13201 ggtgtagaac caatatcgca tacttgaact tttttcaatg agtataaatt aaaacttcta
    13261 attttcaatt cccatcatct gatatgcccg accgtctgaa cttcttttga aaaagccaaa
    13321 tgtgattaga taacgtcttt gttcgacata atcgccacta gaaaatacgt tcagcagtat
    13381 cgaattaacc tctgtttctg tataagtcac tccatggtca aagcgtttca agacttcttt
    13441 caataggatg tatttctttt ttgttgaact tggtacttgt tttacttttc catcattcat
    13501 gaatgtgtct aaaactttca tcttttcttg ctccgtataa gtataaacca tcttctttct
    13561 ccttttgatt ctaagaatac tccatttatg ttagaatatt ctggattttt tattaaagcg
    13621 gttactctta agaaagatat tatcatagtg ctcttttttt tactactact tataggtaga
    13681 tttattctaa tccattattc tcccctacca ataattaaag aaaaaactgg atcagggtgt
    13741 tcaaattaca tgctaagaaa aaagaaccct tgaataccaa gggttctttc tcagtctgat
    13801 aacaatgatt aacgacggat ttctttgata cttgtgaaaa gtcgttaaag tattgatata
    13861 aaaggatttt tattacctct gactacatat tgactaattt gttaaaaaat cgtttttcaa
    13921 gaatcctgta ataccagtgc tttcaccgtt tttttaactc tctaaagaac taaaagcaat
    13981 cagaggtttt accttttcat ttgacgttaa gtggcgataa attttcgaag ttgtttggtc
    14041 agtatggcct aagcgatctt tcaagacttc ccagctacag tgttccgcta ctaagcttgc
    14101 cattgtatga cgagctaaat gagtatgaaa tggcttatta attcctgagc gattcacgca
    14161 ccgtttgacg tgatcattta catactctcg acgataagga acaccacgtt tactagaatc
    14221 aagatacaca aacaaatatg tttcttctat aataaacctg taatgttgcc tcatctcacc
    14281 caaagcctta atcaactgtt gtagcttttc cataactaga ggagttacta gaactcgtct
    14341 agcagaacta actgtcttag tagtttcaat tcgaaaatcc ttactcttac ctttaactgc
    14401 cgtttgctta tagaacagaa tttcatttct tttgaaatct atatctccag gctgtagagc
    14461 aagcgcctcg ctgactcggc agccagtgta aaatagaaca tggaaaagta cagacatcgg
    14521 taattcctca tgaacaatgc cataattcaa aaaagttctc aattcatcga cagtaagaaa
    14581 cttgtcctca atattattta tggcttcttt taaatcagtt accgttaact gatattttgg
    14641 cattctcaca gacttcattg gattttcact tatcaaattc tttctttgac aatatttaaa
    14701 aatcatattt agaacttgaa taagtgattg taaactattt ttggcatagc caacatttgt
    14761 tccattaacg ccacattcct ttaaggtaaa aagcaattct tgaaggcact ctggtgtcaa
    14821 atcagacaac tttttatcac caatccatcg aacaataatt tttaattgtt cctctcgctt
    14881 atcataggtt atttcctttt gttcatttaa cgaacggtaa tattggaacc attgctcacc
    14941 tacaacacta aacttgtcat tgcttgcaac aatttttttc ttaagccttt gttgtttctc
    15001 catttccaga taagcgagtt gcgcctcacg ttttgaatta aagccacgtc ttgttgttcg
    15061 atatgcttga aagtaccatg tgatcctacc agtagaactg atttgcttct tgaatcgatc
    15121 tgttttttcc atttattgat tcctcctatt ttgaaggaac ccaacaagca gaaacccttt
    15181 ggaaatcttt ctatctatgt catcattgta ccgcatctta gaaagaaaat gtaacttata
    15241 aagatcgaag tagtcgcaat ttttctcgat agtcattcga atcactcttt ctcatccatt
    15301 ctaaaacatc atcacgaaaa tagtgtcctc gaatcccgta ccaacaggga ggaaactcac
    15361 caagttttac taaacggttc agtgtactat cacttatctg aaatagtcgc tttaaatcag
    15421 tatttgttaa taccagaggt aaatgactga ctgcaagcaa tttttccgtg taatcttcca
    15481 gttttttttg taaaattgaa tagaactcta tttccagaga attaacattt gttttaactt
    15541 ccaactccat cacccttctc tctcaaaatc aaaacgacca ccacggtacc ccttaaaatt
    15601 gtaaaaaaac actccagtac tccatcaaaa cggactagaa aagaggaatt tttaatatga
    15661 aacaaggaat tgcagacata aaaataatta aagaaattct tgaaaaatct acggctaatg
    15721 caatagcatt cggaacagga atcaacttga gtacagtaaa aaaacttaaa agtggagaga
    15781 gagctgaaga aaaattaaat cttgccgatg ctattaaaat cacagaattt ggcatgaaaa
    15841 atatgccaac caaaatcgaa atatggaaat agtagttttt ctgatctatc agctgttttg
    15901 ccaaagcaca taggaatctc acctcccccc agtaatggcg ggccgatttg gttacggaag
    15961 tatcattatc gtagtcgcga ttctcatatt aaaatttgtc gcttaaaagc taaccttgtc
    16021 atggcgtaac tagagaatca aaatctacat gtactgatag atttgctatt catttgtcaa
    16081 agagcgctag gctattttca gcctatggaa atcctttatt ttgacccgat aaaaaacttc
    16141 catagaatga aaaggaaagg gaaaaaatac tttaacgagt tgtatcaaat tcaagtatct
    16201 tctcaatcaa ttttgtttcc agttgtctac gcatatattc atctacatac ataaccattc
    16261 ctttttcttc ttcataaact ggtttcatgg ctaaccgtga aatataattt tgatattttg
    16321 acagaacgat ggacatggca attgaatcac cagaagctgc ggctttgatt gtcgttagtg
    16381 gtatgaatgg tatatatcgt ttattttgct tcatatttga cacccaaata ctttcctagc
    16441 tcttccaaag ccatttgtcg ttggtaccaa acactgccga gacttttccc aataatatcg
    16501 ccaatttcac gatcattaag accgatgagg aaggacataa aaatgatttc ttgtctttga
    16561 ctaggtaatg ctgcaatcgc atttgccaac tcctcattgg aaataaagat tttcatacca
    16621 aggacaataa aagtaccact actattcaaa tttggttcca aataatagaa ctctgtacct
    16681 aacccttttt gttccaatat ttccattgat atttgccgtt ggcgaaatct agcgtacttc
    16741 ttttgaatat ttaacgcctc attgcagact actttcttac aaaaactgtc aaacgtatgt
    16801 tgaatgtggg tttcctctga gtcgcccata atattctccc cctcaaaaaa ttcattcaac
    16861 tcgttttact tctatccctt tccattatta ctacaatcta gga
//
LOCUS       DAFEOM010000057        16700 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.060, whole genome shotgun sequence.
ACCESSION   DAFEOM010000057
VERSION     DAFEOM010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.060"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            176..403
                     /locus_tag="LP524_002040"
     CDS             176..403
                     /locus_tag="LP524_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBL2235069.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            419..1585
                     /locus_tag="LP524_002041"
     CDS             419..1585
                     /locus_tag="LP524_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBL2235070.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            1820..2863
                     /gene="hrcA"
                     /locus_tag="LP524_002042"
     CDS             1820..2863
                     /gene="hrcA"
                     /locus_tag="LP524_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HBL2235071.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            2957..3520
                     /gene="grpE"
                     /locus_tag="LP524_002043"
     CDS             2957..3520
                     /gene="grpE"
                     /locus_tag="LP524_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBL2235072.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDRYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            3571..5400
                     /gene="dnaK"
                     /locus_tag="LP524_002044"
     CDS             3571..5400
                     /gene="dnaK"
                     /locus_tag="LP524_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBL2235073.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGKRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            5551..6717
                     /gene="dnaJ"
                     /locus_tag="LP524_002045"
     CDS             5551..6717
                     /gene="dnaJ"
                     /locus_tag="LP524_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBL2235074.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(7085..7822)
                     /locus_tag="LP524_002046"
     CDS             complement(7085..7822)
                     /locus_tag="LP524_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBL2235075.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(7824..9326)
                     /locus_tag="LP524_002047"
     CDS             complement(7824..9326)
                     /locus_tag="LP524_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HBL2235076.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIEKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            9585..11579
                     /gene="uvrB"
                     /locus_tag="LP524_002048"
     CDS             9585..11579
                     /gene="uvrB"
                     /locus_tag="LP524_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBL2235077.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            11589..14405
                     /gene="uvrA"
                     /locus_tag="LP524_002049"
     CDS             11589..14405
                     /gene="uvrA"
                     /locus_tag="LP524_002049"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBL2235078.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            14668..15933
                     /locus_tag="LP524_002050"
     CDS             14668..15933
                     /locus_tag="LP524_002050"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HBL2235079.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYAGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            15937..16668
                     /locus_tag="LP524_002051"
     CDS             15937..16668
                     /locus_tag="LP524_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HBL2235080.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
BASE COUNT         5504 a         2901 c         3661 g         4634 t
ORIGIN      
        1 tttttatgaa taaaataagg acaaaattgt ttaggaagaa tcatgagtgt aaaaaaatca
       61 tccaagaagt cgattcttgg attttttagt ggatttgttc gccaaatacg tttagtatgc
      121 gtatatgaga ggttgtaagc aaagcgttgt ggtctgagca cttaaggagg aatctgtgaa
      181 aatagctttt catatttttg cagattttga cttaatgccg aaggtcagct ttgcgattac
      241 cgtttatcga gaggctgtaa aagcaccgtg tagctccgat gacaataagc aagcattttt
      301 agagctaggt ttccttgatt ctaaatatgc ggcttatttc aatgagttgg tgcttgaacg
      361 cgattttaag aaggtcgaaa acaggcacga cttttcgacg taaggagaaa gaatttcaat
      421 gatagaaaca ataaatcccg cagaaaacaa ggtttctgcg tacattcata tcccattttg
      481 cgagcatatc tgctattact gtgactttaa taaagttttt ttagaagggc agccagtaga
      541 tgagtatgtg gaaatgctgc tgaatgaaat gagattaatg ctggaaagtc atcctacagc
      601 gaatttggaa acactttatg taggaggcgg aacaccaact tctttatctg ctaaacaact
      661 tgatcgacta ttatcaggtg ctagagagat cctgcctttt caaaatggta atgagtttac
      721 tgtagaagcg aatcctggtg atttgacccg tgaaaaactt caagtgatga aaaactatgg
      781 cgtcaatcgt ttatccatgg gagtgcagac atttgacaat cgcttattga aaaaaattgg
      841 acgtaaacat actgcagaag atgtgtatca aacaatgcaa tttttagaag cagaaaattt
      901 taccaatgta agcatcgatt tgatttatgc attaccggga caaacactag aaggatatcg
      961 ggatactttg gaaaaagctt tggctttgga tctgcctcac tattctttgt actcattgat
     1021 cctcgaaaac aaaacaatgt tcatgaactg ggtacgacaa ggaagactcg aactgcctga
     1081 tcaggaaacg gaaaccagaa tgtttgaaga gacgattgaa gcaatggaaa agcatggacg
     1141 tcaccaatat gaaataagta actttgggct ctccggacat gaatctcaac ataacctcat
     1201 gtactggaac aatgaccact attttgggtt tggagctggt gctagcggtt atcttggaga
     1261 tattcgttac cgcaacaaag gaccgatcca acactattta cgcccattac gagcaaatga
     1321 acgcccggta ttggaagaag aaaaattgac acgaaaaaat caaatagaag aagaaatgtt
     1381 cttaggacta agaaaaaaaa cagggattac taaaaaacat ttctatgatc gctatcaaaa
     1441 aacaattgaa tcggtgtacg gtgatgtgct aagggaatta gagcaagaag ggttacttgt
     1501 taatgaagaa gaccgtgttt atttgacacc aagagggact tttttgggga acaatgtgtt
     1561 tgagcgcttt ttatttgaaa aatagtttga agaatatttg ttttttatcg aaacttgaaa
     1621 agaaaaagtt tagaaagttt gacaatgagg tattgaagta ttgatataac tggatttaca
     1681 tcaattattt agaattagca ctcttgtgtt tagagtgcta aaaaatggag tctttctctt
     1741 gacaggagaa gggcttcttg ctatatttat aagtgaatta gcacttagaa tatatgagtg
     1801 ctaatgagag gtgaggaaga tgttaacaaa gcgacaaaac gacattttac gtctgatcat
     1861 ccaacactat acgagtagtg gcgtgcccgt tggttccaaa acattaatgt ctgagggagt
     1921 caaggccagc tcagcaacga ttcgcaatga tatgaaagca ttagaagatg aaggattgct
     1981 gttgaagact cactcttctt ctgggcggat tccatctgct ttaggctatc gttattacgt
     2041 ggatcatttg ttaaaacctg ctcgcgtagc agaagatgat ttgctgctga ttcgtcagtc
     2101 tctaggcaga gaatttcatg aaatcaatga gattattaag caatctgctg agatattatc
     2161 tgagctgaca agctatacga ctttttcatt gggacctgaa ataaaagacc gccgactgac
     2221 tggctttcgt atcgtaccct tgaataatcg acagattatt gctatcgtcg taacagatat
     2281 ggggaatgta gaaagccaag tgttcactct tcccgaatat atgggaagtc aggaactaga
     2341 aaaaatggta cagattgtaa acgaccgtct agtcggagat cctctgatga cggtgtacca
     2401 taaacttcgt acggagattc cgatgattct tcataaatat ttccagacga cagaagggat
     2461 gtctcagtta tttgacaccg ttcttggtca tgtttttgaa gaaaaggttt atgtcagtgg
     2521 acaaatgaat ctcttggatt atgagccccg tcaagatctt gatcagttca aatcgatgtt
     2581 ttcttttatg aaagactcag acggacttac acaaatgatc actccgatgg atagcaatat
     2641 tcatatcaag attggtgctg aattaggcaa tgatttattg cagaatatga gtatgattca
     2701 agcaagttac gagatagatg gtcatggaag aggtacaata gccttgttag ggcctactag
     2761 tatgccttat tctcggatgt tcggtctagt cgatgtttat cgaagagaac tagcagctaa
     2821 acttgctgat tattaccgct cgcttgactt gtcaaactcg taatgtgaat aacgaagcta
     2881 tttgcagctg atcgttctct ctttcttagt aagagcaatc aaacagctga ctgaataatg
     2941 aaaggaagac tgtatcgtga aagaaaatca agaagaacta gataaagaaa tgacagatgc
     3001 tcagccagaa ccggaaatcg atgtcgaagc tgcagaagat tcgggtattt cagaagctga
     3061 agcggaagaa ttcgaaacag cgaaattaaa agctgagtta gaagaaatgg aagatagata
     3121 cttgcgggct cgtgctgaaa tcgcaaatat ggcaaatcga ggaaaaaatg agcgtgagca
     3181 attacaaaaa taccgttcac aagatttagc caaaaaatta cttccatcaa ttgacaacct
     3241 ggaacgtgca ttagccacag aagtgtcaga tgatcaaggg gcaggtttga aaaaaggcgt
     3301 ggaaatggtc ttggaaagtt tgcgtaacgc actggaagaa gaaggaattg aaaaaatccc
     3361 tgctaaagga gaagcctttg atcctaatct tcatcaagca gtacaaactg ttccagcgac
     3421 tgaagataca ccagccgata cgattgtaga agtactacaa gaaggctaca aattacatga
     3481 tcgcgtgtta agaccaacta tggtcattgt tgcacaataa ttgtgcggat ataaaactta
     3541 cactttcaat taaagaagga gacgttaatt atgagtaaaa ttatcggtat tgacttaggt
     3601 acgactaact ctgcagtagc agtattggaa ggaaacgaag caaaaatcat cgctaaccca
     3661 gaaggaaaac gtacaacacc ttctgttgta tcattcaaaa atggagaaat ccaagtaggg
     3721 gaagttgcaa aacgtcaagc tgtgacaaac ccaaatacga tttcatcaat caaacgccat
     3781 atcggtgaag caggttacaa agtagatgta gaaggtaaat catatacacc acaagaaatc
     3841 tcagcaatga tccttcaata tatcaaagga ttcgcagaag actatttagg tgaaaaagtc
     3901 gaaaaagcag ttatcactgt gccagcttac tttaatgatg cacaacgtca agcaacaaaa
     3961 gatgctggta agatcgctgg tctagaagta gaacgtatcg tcaatgaacc aactgcagct
     4021 gctcttgcct atggtttaga taaaacagat cgcgacgaaa aaatcttagt attcgacctt
     4081 ggtggcggta cttttgacgt atctatcctt gaactaggag acggtgtctt tgatgtattg
     4141 tcaacagctg gcgacaacca tctaggtggg gacgactttg ataacaaaat catcgactac
     4201 atggtagcag aattcaaaaa agaaaacagc attgacttgt ctcaagacaa aatggcttta
     4261 caacgcttga aagacgctgc tgaaaaagcg aaaaaagatt tatctggcgt atcaagcaca
     4321 caaatcagct tgccatttat cacagcagga gaatctggac cattgcattt ggaaatgaca
     4381 ttgacacgtg caaaatttga tgaattaaca gctgacttag tagaacgtac aaaagtacca
     4441 gtacgtcaag cgttaaaaga tgcaggtctg tcacaatcag aaatcgacga agttatttta
     4501 gtcggtggat ctactcgtat ccctgcagtt gtagaagcag tacgtaaaga aacaggaaaa
     4561 gaaccaaaca aatcagttaa ccctgatgaa gtagttgcta tgggtgctgc tatccaaggt
     4621 ggcgtgatca ctggtgatgt gaaagatgtt gttttacttg acgttacacc attgtcatta
     4681 ggtatcgaaa caatgggtgg agtgttcact aaattgattg accgcaatac aacgattcca
     4741 acaagtaaat cacaagtatt ctcaactgcg gcagataatc aaccagcagt agacatccat
     4801 gtattacaag gtgaacgccc aatggctgct gacaacaaaa ctctaggaag attccaattg
     4861 acagatattc cagcagctcc tcgtggtatt ccacaaatcg aagtaacatt cgatattgat
     4921 aaaaacggga ttgtaaatgt atctgcgaaa gatctaggta cacaaaaaga acaaaaaatc
     4981 acgatcaaat cttcttcagg tctaacagat gaagaaatcg aacgtatggt gaaagatgca
     5041 gaagcaaacg ctgaagcaga taaagctcgt aaagaggaag ttgacctacg taatgatgta
     5101 gacgcattgc tattctctgt ggacaaaaca ttgaaagaat tagaaggcaa agtggacgaa
     5161 gaagaagtga aaaaagcaga aacagctcgt gacgaattaa aagccgctgt tgaagcaaac
     5221 aacatcgagg aaatgaaaac taaacgcgat gcattgaacg aaatcgttca aaacttgact
     5281 gttaaactat acgaacaagc tgcacaacaa caagctcaag aaaatccaga agcagctcaa
     5341 ggcggagcag atgatgtcgt tgatgctgat tttgaagaag tcgatggcga cgataaataa
     5401 tcagttcact gaatttcgag agaaggccaa agttgctact ttggcttttt cttttgaaaa
     5461 atagtttgaa gtatcggttt ttgtttcaaa caaaatatga tatgataaca agcgacgtga
     5521 aaatgatgag aaccaatagg aggaaagcct atggcaacaa aacgtgatta ttatgaagtc
     5581 ttagggctct caaaaggagc atcagaagat gaaataaaga aagcttatcg taagctttcg
     5641 aaaaaatacc atcctgacat caataaagaa cctgatgcgg aagaaaaatt caaagaagta
     5701 tcagaagcat atgagatttt aagtgacccg caaaaacgcg ctgcatacga ccaatacggg
     5761 catgcaggca cagatccaaa ttacggtgct ggtggagctg gtggcggatt tggtggattc
     5821 ggcggcttct ctggtggcgg cttcggtggt tttgaagata tcttcgactc tttctttgga
     5881 ggaggcggtc gatcagtcga tccaaatgcg cctcgtcaag gtgctgattt gcaatatacg
     5941 gtcgacttgt cttttgaaga agcgattttt ggtgtagaaa aagaaatcaa gtataatcgg
     6001 gaagaaatct gtcatacttg tggtggaaac ggtgcaaaac caggcacaca accaactact
     6061 tgtcataaat gtcatggtgc aggtacgatc aatgtagaac gccaaacgcc acttggacga
     6121 gtgatgagcc gtcaaacttg tgatgtttgt catggcacag ggaaagaaat caaagaacct
     6181 tgtccaacct gtcatggctc aggacatgag aagaaagcac attcagttaa agtgaatgta
     6241 ccagctggtg tagaagaagg acaacaaatg cgtttagctg gtcaaggaga agctggagaa
     6301 aacggcggac catttggtga tttgtatgtg gtctttcgtg tagaagaaag tgatatcttt
     6361 gatcgtgaag gttctgaaat ttactatgaa ttgcctttaa gttttgtaca agcagctttg
     6421 ggggatgaag tccaagtacc aactgtccat ggagatgtga aattgaagat ccctgctgga
     6481 acacagacag gtacgaactt ccgtttacgt ggaaaaggcg ctccgaagtt acgtggtggt
     6541 gcaacaggcg accaacatgt caaagtaaaa ctgatcacac cgaaaaatct aaatgaacaa
     6601 caaagagaag cattacgtgc attcgctgaa gctggcggca tcaaagttga agaacagcaa
     6661 gaagacggtt tcttcgataa gtttaaagat gcttttggcg ggaaaaagcg gaagtagttt
     6721 ttctagtaaa aatataaagt agacttgccc aaattttcta tttctagtaa aaacgctaag
     6781 tcattaataa ttaaaaacca gacgaacaat catacgttcg cctggttttt ttgtagttgt
     6841 catgtttgta ccaacaaaaa agccagcttt agaataagat aatcatcata gtttctgaag
     6901 gtggtatcga ctatgtactt gatatcgtca atgaaccgat ggtactttct tatgcgatgg
     6961 aacaggtaga gcgattgctt gtttgtgcag gagaacaagc gatgcggctg gtacagttaa
     7021 gtgaaaaaac aagaaactaa aaatgcccgg tcgtcatgaa gcaaccgggc atttttggtt
     7081 tttattaaat attcaatact ttattcaaga aatcttttgt ccgttcattt tgcggatttt
     7141 caaagatttg ttgaggcgtt ccgtcttcta aaaagtttcc gtcgtcaatg aacatgacac
     7201 gattggccac ttctttagcg aagcccattt cgtgcgtaac gataaccatc gtcatacctt
     7261 gttctgctaa ctttttcatg acgttcaaga catcaccgac catttcgggg tcgagtgcag
     7321 atgtaggctc atcaaaaagc atgatgtcag ggttcatcgc tagagcacga gcaatagcca
     7381 cacgctgttt ttgtccacca gaaagcattt caggatacat atctttttta tcttctagac
     7441 caactgtctg cagtaatttg attgcttttt tctctgcttc ttctctagat tctttcttca
     7501 ggtccatcgg tgctaaaatg atgttttcca aaatagaaag atgagggaat aggttaaagt
     7561 gctggaatac cattccgata tgttgtctca ccaagttgat atctgtgctt ttatccatca
     7621 agtgggcgcc gtcaataata atttcaccgt cagaaggttc ttctaaacga ttcaagcaac
     7681 gcaaaaatgt actttttcct gaacctgaag ggccaatgat acagacgaca tctccttcgt
     7741 tgatggatac attgatgtcg tttaatactg tgttatcgcc gtattttttg actaaatgct
     7801 caactaagat tttttctgcc atcttacttc accttctttt ctaaaacttt tgctaatttt
     7861 gtcaatgctg tgatcaaaat caagtacatg atcgcaatga tcaagtaaac atacgtactt
     7921 tgtgttgtac gtgcaacaat gatcttacca gtttgcagca attcgactac accgattgcg
     7981 gaaatgatcg ttgtgtcttt taatgagata acgaactgat tgacgaatga tgggatcatg
     8041 atgcgtattg cttgtggcaa aatgattttt tgcattgtcc gattataaga aagacctaaa
     8101 cttctggaag cttccatttg tccgacagga acggcgttga tcccgcctcg aacaatctct
     8161 gcgatataag cacttgcgtt aagagtcaag gtgataacac cagcaaggaa gtcaggaatc
     8221 gtaaatccaa cgataccagg caacccaaag aaaataaaga acgcaagaac catcattgga
     8281 attcctcgga tgatatcgac ataaatacta gcaattgtgc gaagagcgcg tattggtgct
     8341 acactgaata atccaaaaat cacaccgatt actatagcca aagcaaagga gatcaaagtt
     8401 aggagaattg ttctccaaag tcctttcaac aatgcttgat agttgttttg gataagtcca
     8461 agaaatgttg attcatcagc agtggaagac tcactagaag tttctgaacc atccgctaca
     8521 tattctttga taatcttatc gtattcgcct gttcttttta attctttcaa tccttcattg
     8581 aacatattaa gaagctcagg attttgtcct tttttaacgg caaatgcata ttttccgcca
     8641 gattcttttt cgatcggtgt ttccaattct tgtccttgtt tgatcgcata accgattacc
     8701 ggataatcat ccatcattgc atcgattgcg ccaactttta acccgtcgta caattgatca
     8761 gcagcatcaa aatatttgat agaatagcca tattcagatt catggtcgct taaaaattca
     8821 gcactttcgg taccaacttt tgctccgact gtttttcctt tcaaatcttc ataagacttg
     8881 atcgaagtat ctcctttttt gacagctaat tggatcccgc tatcaaagta agaatcagaa
     8941 aaatcatagc ttttctttcg ttcatctgtt acagtcattc cggcaatcat tccgtctgct
     9001 tgtcctgatt ccaaagcttg caaagcagca gagaaaccac ggtagtccat tcggatgtta
     9061 aatccttgca gtttggcgat gttttccatc aattttacat cgatgccttc gtactcgccc
     9121 tttgcgtttt gaaattcaaa tggagcaaaa gcagagtcac tggcaattac gtatacgtct
     9181 tttttgggct ctattttttg cattgtctcg cttgtgcttg actctgccgt tgtagtgttg
     9241 tcagcttgtg caacatgacc aaacgcaagt gtcacaagtc ctgtcattaa gaaaacaaat
     9301 gagtgaaata aatgctttct tttcataatc aacctcctaa atttccaata ttttattatt
     9361 ataggcgaaa agtgctgaat cagacaagac tgtaagctcg aaaaaatgaa aaaaatgcga
     9421 gtttctaaca ttttatgtta taaaacgtaa catgtataat aagataaaaa agtggcgaaa
     9481 gtatgttcgg tgtggtataa ttttttagta aggaatgcct ttgctttcct tttcttttta
     9541 ttggtcgtaa aatgaaaagg aatgaatgaa aaggaggacg aagaatgatt gagagagata
     9601 cctcccgaca ctttgagctt gtctcgaaat accaaccggc tggtgatcag cctgaagcga
     9661 ttaaccaatt agtggatggc gtagtcggcg ggaaaaaagc acaaatctta ttaggtgcga
     9721 ctggaacagg gaaaacatat acgatttcca atcttattga gaaagtgaat aaaccaacac
     9781 tgattattgc tcataataaa acattggctg gtcagctgta cggggaattc aaggaatttt
     9841 tccctaacaa tgctgttgaa tattttgttt cttattatga ttattatcag cctgaagctt
     9901 atgtgccttc aagcgatacg tatatcgaaa aagattcaag tgtaaatgat gagatcgaca
     9961 aattacgaca ttcggctact agttcattat tagaaagaaa tgatgtgatc gtcattgcat
    10021 ccgtttcatg tatttttgga ttaggttcgc cttttgagta ccaaaaacaa gtagtttcga
    10081 ttcgacaggg agcagaacta gatcgcaatc agttgattcg tgacttagtc agtatccaat
    10141 tcgaaagaaa tgatatagat tttcagcgcg gtcgttttcg agtgcgcggt gatgtcgtgg
    10201 agatttttcc ggcttcaaga gatgaacgtg ccttacgtgt ggagtttttt ggagatgagg
    10261 tcgaacgtat ccgagaagtc aatgcactga caggtgaagt tttaggagaa acggaacacg
    10321 tggcaatctt cccggcgact cactttgtga cgaatgatga acatatggag catgccgtag
    10381 cgaatatcaa agcggaatta gaacagcgtt taactgtctt gcgaaatgaa aacaaacttc
    10441 tggaagctca acgtttagaa cagcgaacca attacgatat tgaaatgatg ttagaaatgg
    10501 gctacacgtc gggaattgaa aattattctc ggcatatgga cggacgtaaa gaaggtgaac
    10561 cgccttatac gctattggat ttctttcctg aagatttttt gatcgtggcg gacgaatcgc
    10621 acgtaaccat gcctcaaatc agaggaatgt ataatggtga ccgagcgaga aaacagatgc
    10681 tagtagatta cggtttccgt ttgccatctg ctttagacaa tcggccactt cggttagaag
    10741 agtttgaaaa acatgtcaat cagatcattt atgtctctgc gacgccagga ccatatgaac
    10801 atgaacaaac agataccgtt atccaacaaa tcatccgtcc gacagggctg cttgacccag
    10861 tgatcgaagt tcgtccaatc atgggacaaa tcgatgatct agtaggcgaa ataaatgaac
    10921 gagtcgaaaa agaccagcgg gtgtttgtaa cgacattgac gaaaaaaatg gcagaagacc
    10981 tcacagatta tttcaaagaa ttagggatca aagtcaaata tttgcatagt gatatcaaaa
    11041 cgctggaacg aacagaaatc attcgagatc ttcgattagg tgaatttgat gtgttagttg
    11101 gaatcaattt gcttcgtgaa ggattagacg tacctgaagt ttcccttgtt gctatcttgg
    11161 atgcggataa agaaggcttt ttacgtagtg aacgttcctt agtccaaacg attggacgag
    11221 ctgctcggaa tgaagaaggt aaagtaatca tgtacgcaga caaagtaaca gattctatgc
    11281 gtctagcgat ggatgaaacg tcaagacgtc gtacgattca gcagaagtac aatgaagaac
    11341 atgggatcgt tccaaaaacg atcattaaag aaatccgaga tttgatttct attacgaaag
    11401 aatcggaaga tgatacaaaa gaagcggtac aagtctcgta cgaagaaatg acgaaggaag
    11461 aaaaagacac gttattgatg aaattagaaa aagaaatgaa agatgcggca aaagctttgg
    11521 attttgaaac agccgcgaat gtgcgagata tgattttaga attaaaagct tcaaagtagg
    11581 aggacaacat ggctaacgat aaaattacga ttcatggtgc acgtgcccat aacttgaaaa
    11641 atatagatgt aacgattcca cgagataaat tcgtggtcgt tacaggtttg tccggttctg
    11701 gaaaaagttc cctagcattt gatacgctgt acgctgaagg gcagcggaga tacgtagaga
    11761 gcctttctgc ttatgctcgt cagtttttag gacaaatgga taagccggac gtagatagca
    11821 tcgacggatt gagtccggcg atttctattg atcagaaaac aacaagtaaa aatcctcggt
    11881 cgacagtagg tactgtgacg gaaatcaatg actatttacg cttgttgttt gcacgtgtag
    11941 gacatccgat ttgtcccaac gatcatatcg agattaccag tcaatcagta gatcaaatgg
    12001 cagatcaagt aatggctttg cctgagcgca caaagatcca agtgttggca ccgatcgtgg
    12061 taaagaaaaa aggacaacat aaaaaaatat tcgaacgaat ccaaaaagag ggatacgtcc
    12121 gcgttcgagt cgatggtgag atctatgatt taagtgaagt gcctgagctg gagaaaaaca
    12181 aaaaacatga tatcgcgatc gtcatcgacc ggatcattgt gaaagaaggg attcgttctc
    12241 gcctatttga ttctttagag gcggccctgc gattagcaga cggctatgct ctggttgacg
    12301 tgatcggtga agaagaaatg ttgtttagcg aacactatgc ctgtccttat tgcggattta
    12361 ctgttggcga actagaacca agactgttct cattcaatgc gccttttgga gcttgtccag
    12421 actgtgatgg tttaggtgtc aaattagaag tcgatcaaga tttagtgatt ccagatagaa
    12481 cgaagacact tagtcaaggt gctatcgttc catggaatcc aatcagctct cagtattatc
    12541 ctcaaatgtt agcgcaggct tgtgaaagtt tcggtatcga tttagataca ccattcgaag
    12601 aattaccgga agagcatcag gagatcatct tgaatgggac agatgatctt ttccatttcc
    12661 attatgaaaa tgattttggt ggcgttcgcg atgtagaaac gactttcgaa ggggtattga
    12721 agaatatcga gcgtcgttat catgatacga acagtgattt cacgcgtgag caaatgcgtc
    12781 tttatatgac agaattaact tgtcagacat gtaaaggtta tcgattgaat ccccaagctc
    12841 tatctgttca aatcaatgga acgaatatcg ggcaaacaaa tgagttgtct attcaaaaag
    12901 cagtagattt cttctcaaac ttaacgttat ctgaacaaga gaaagtaatt gccaaaccga
    12961 ttttaaaaga agtaaatgac agactttcat tcttggaaaa tgtgggacta gactatctaa
    13021 ctttgagtcg cgcttccgga acattatctg ggggagaagc gcagcgaatc agattggcta
    13081 cacaaattgg atcgaatctt tctggagttt tgtatatttt agatgaacca tcgatcggat
    13141 tgcatcaacg cgacaacgat cgactgattg gctcattaaa gaaaatgcgt gatcttggca
    13201 acaccctcgt tgtagtcgaa catgatgaag atacgatgtg ggcagcagat tatttgattg
    13261 acgtcggacc tggggccgga gaacaaggcg gacaaatcgt ggctgcaggc actcctgaag
    13321 aagtagctaa tgatgaaaat tctttgacag ggaaatatct ttcagggaaa aaatcaatcc
    13381 ctgtaccaaa agaacgccgt aaaggtaatg ggaaagcaat caagatcact ggtgcttcag
    13441 aaaacaatct gaaaaatatt agtgttgaat ttccattagg agaatttgta gcagtcacag
    13501 gtgtgtctgg ttctggaaag agtacattgg tcaatagtat cttaaagaaa tcgttagcgc
    13561 aaaaattaaa taagaattct gctaagccag gtaaattcaa gacaatttcc ggctacgaaa
    13621 gtatcgaaaa gatcatcgat atcgatcaaa gcccaatcgg ccggacgccg agaagtaatc
    13681 cagcgactta tacaagtgta tttgatgata tccgtgggtt atttgctcaa acgaacgagg
    13741 caaaaatgcg gggttataag aaagggcgtt ttagtttcaa cgtaaaaggc ggtcgttgtg
    13801 aagcttgtcg cggggatgga attattaaga tcgaaatgca ctttttgcct gatgtctatg
    13861 ttccttgtga agtatgtcat ggtaaacgat ataattctga aacattagaa gtgcattaca
    13921 aaggaaaaag cattgctgat attttggaaa tgacagtaga ggatgctgta gaattcttca
    13981 agcacattcc aaagattcat cgcaaactgc aaacgattgt tgatgttggc ttaggttatg
    14041 tgacgatggg gcaaccagca acgacattgt ccggtggtga ggcacaacgg atgaaacttg
    14101 ccagtgaatt gcacaaaatc tctaatggaa agaatttcta tatactagat gaaccaacga
    14161 caggacttca tagcgatgac atcgcccgct tgttgcatgt attacaaaga ttagtagatg
    14221 ctggtaacac agttttagtg attgaacaca atctagatgt aatcaaaaca gcagattata
    14281 tcattgattt aggaccagaa ggtggagaag gtggaggaac gatccttgcg actggaacac
    14341 cagaagaaat cattaacgta aaagaaagtt atacaggtca ctatttgaaa aaaataatgg
    14401 tataaagagg atagatagct gagatagtta aaaaaacata aaaaaatgaa tttttgcttg
    14461 ttttattaat atcagtgttc tagaatcatt acatggtagt tctttctaca ctgattggta
    14521 tagataaaaa cttcctataa gttcaatccg ttcaaaaaag aattgagcaa tcggaggact
    14581 ttcctttgat tgcttttttc tttatggtat gatattcctt gtgtagttgg aaaagaaaaa
    14641 caagagatta tgagtaagtg agagataatg atgaacagta ttgaaaatga agaatttatc
    14701 cctatattat taggaagcga catgaacgtt tatggtatgg cacgttcttt taatgaagca
    14761 tacggcaaaa tctgccaagc atatgcttcg gatcagttag cccctacccg ctacagtaaa
    14821 attgtcaatg tagaagttat tccaggattt gataaagacc ctgtatttat cgagacgatg
    14881 cttcgtttgg caaaagaaag atattctgat aagagtaaaa aatatctttt gattgcttgt
    14941 ggtgatggat atgcagaatt gatctcccaa cataaacaag agctgtcaga atattttatc
    15001 tgtccatata ttgattattc tttatttgaa aggctgatca ataaagtaag cttttatgaa
    15061 gtttgtgaag aatatgatct tccgtatcct aaaacattga tcgtcagaga agaaatgtta
    15121 gtaaatggac atttggaaca ggaattgcct tttgaattcc cagttgcttt gaaacctgca
    15181 aacagtgtgg agtatctttc tgtccaattt gaaggaagaa aaaaagcttt tattttagag
    15241 actagagaag agttcgattt aattttaggc cggatatacg aagccggtta taaaagtgaa
    15301 atgattgtac aagattttat cccgggtgat gatagtaata tgcgtgtgct aaatgcgtat
    15361 gtagatgaag atcatcaagt tcggatgatg tgccttggtc atccactatt agaagatcct
    15421 acaccagcat cgattggtaa ttacgttgtg atcatgccag attataacga aaaaatctat
    15481 caaaccatta aggcattttt ggaaaaaatc gaatatgcag gctttgccaa tttcgatatg
    15541 aagtatgatc ctagagatgg agaatataaa ttatttgaaa tcaatttgag acaaggtcgt
    15601 agcagtttct ttgtgacatt gaacggcttg aatctggctc gttttgtcac agaagatcga
    15661 gtgttcaaca aaccctttgt tgaaacgacc tatggaacga atcaatcaga taaagcaaga
    15721 ttatggatgg gtgtgcccaa aaaaatcttt ttagaatatg ctcgggaaaa tgaagacaaa
    15781 aaactagctg agcaaatgat caaagaaaat cgttacggta ctactgtatt ttatgaaaaa
    15841 gaccgatcaa tcaagcgctg gttgttgatg aaatatatgt tccataacta tattccaaga
    15901 ttcaaaaagt attttcatgt gaaagaaggc tagttcatgg agaatttttt cagtatttta
    15961 ggcggaatgg gcacgatggc gacagaaagt tttgttcgct tgatcaacca ccgaacgaaa
    16021 gctacaaaag atcaagaata tttgaattat gtcttattca atcatgcaac agttcctgat
    16081 cgtacagctt atattttaga tcgatcagaa gaaaatccaa tgccgtttct gctggatgat
    16141 attgagaaac aaaatttatt gcgaccaaac ttcattgtgt tgacttgtaa caccgcacac
    16201 tacttttttg aagagttaca agcggcaacg gatattccta tcttgcatat gccaagagaa
    16261 gcagcaaatg aacttgtccg tcagcatacg acagggagag tagcgattct aggaacagaa
    16321 ggaagtatga aagctggaat ctatgaacgc gaagtcaaaa atcttggttt cgaaacagtg
    16381 attcctgata ctgcgcttca agaaaaaatc aattacctga tctatcatga gatcaaggaa
    16441 tcggattatt tgaatcaaga gctctattac gaaatcttag aggaagcggt agagcgccta
    16501 aactgtgaaa aagtcatcct aggttgcaca gaattatctt tgatgcatga atttgctgaa
    16561 gataatcatt acccagtgat cgatgctcaa tctattttag cggatcgaac gatcgaacgt
    16621 gcattagctg aaagaagcga ggcattggat acagcatcgg aaaagtaaaa aaattggatt
    16681 tggggcaaat atcctaaaag
//
LOCUS       DAFEOM010000058        16669 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.061, whole genome shotgun sequence.
ACCESSION   DAFEOM010000058
VERSION     DAFEOM010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.061"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            <1..1350
                     /locus_tag="LP524_002052"
     CDS             <1..1350
                     /locus_tag="LP524_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL2235081.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1484..2827
                     /locus_tag="LP524_002053"
     CDS             1484..2827
                     /locus_tag="LP524_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL2235082.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2939..4390
                     /locus_tag="LP524_002054"
     CDS             2939..4390
                     /locus_tag="LP524_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBL2235083.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            4739..5371
                     /locus_tag="LP524_002055"
     CDS             4739..5371
                     /locus_tag="LP524_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HBL2235084.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            5959..7398
                     /locus_tag="LP524_002056"
     CDS             5959..7398
                     /locus_tag="LP524_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL2235085.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            7693..7911
                     /locus_tag="LP524_002057"
                     /pseudo
     CDS             7693..7911
                     /locus_tag="LP524_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8160..8375
                     /locus_tag="LP524_002058"
     CDS             8160..8375
                     /locus_tag="LP524_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235086.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(8592..8848)
                     /locus_tag="LP524_002059"
                     /pseudo
     CDS             complement(8592..8848)
                     /locus_tag="LP524_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9035..9946)
                     /locus_tag="LP524_002060"
     CDS             complement(9035..9946)
                     /locus_tag="LP524_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL2235087.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(9965..10969)
                     /locus_tag="LP524_002061"
     CDS             complement(9965..10969)
                     /locus_tag="LP524_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBL2235088.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(10966..13089)
                     /locus_tag="LP524_002062"
     CDS             complement(10966..13089)
                     /locus_tag="LP524_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL2235089.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(13094..15541)
                     /locus_tag="LP524_002063"
     CDS             complement(13094..15541)
                     /locus_tag="LP524_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL2235090.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(15528..15917)
                     /locus_tag="LP524_002064"
     CDS             complement(15528..15917)
                     /locus_tag="LP524_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL2235091.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(15977..16480)
                     /locus_tag="LP524_002065"
     CDS             complement(15977..16480)
                     /locus_tag="LP524_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBL2235092.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(16493..16654)
                     /locus_tag="LP524_002066"
     CDS             complement(16493..16654)
                     /locus_tag="LP524_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235093.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
BASE COUNT         5384 a         3107 c         3072 g         5106 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1441 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1501 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     1561 ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat gtgggacatc
     1621 gttatttata aaaggagaag taagtaattt agaagatctt ttttatcaaa atagaatgac
     1681 actaaattct gagataaaaa agttatttct agagacagaa gttgacaaaa ttgatattca
     1741 aaataaaaag attagttgtg taaaaaaaga tggtacaaga ttctctcaag aatatgacaa
     1801 agttatatta gctacaggtt caaagcaaat tatgctagat ataccgggga ataatttagc
     1861 taatattttt tcgataaaga attttccaaa tgcaaaagag atttttaaag ctttggatga
     1921 tagtactatt aaaaaagtag gaattattgg agcaggatat attggagttg aaattgctga
     1981 agccataaga aaaagaggga aagaagtata tcttttcgat gttgcagata gagtgttgtc
     2041 gacttattac gaccgtagtt tttcagataa agttgaagaa atactatcaa aaaatggaat
     2101 acatctttgt ctatcagaaa gtcctaaaaa atatttagga agaaaaaaga ttgaaaaaat
     2161 agtcactaca agtggtaccc aatactctat ggatatggtc gtacaagcta taggatttct
     2221 atctaatagt ccaattgggg aaaaggaact cttgagagat gttagtggag catatctaac
     2281 taatgaatac cagcaaacaa atataaaaga tatatatgct attggggatt gtgctacaac
     2341 tttttttacg tccattaata gaagtagtgt tgatttttct atttctaatg ctctaagaac
     2401 agcttatatt gcaactcaac atattaacaa tcaagatttt acaccttctg gttcccaatt
     2461 tacaaatggc ttttctatat ttaattacaa tttttacgca gcaggtttga atttaaaaac
     2521 agcacgatta ttaagtgagt ctatagatta tatagaaata gaagatttca ttagacctgc
     2581 ttttttgaga aaaaatagtc gtgtaaaatt aagaattagc tttgaaaaaa atacaaagag
     2641 aatagtaggt gtacaacttt gtagtcaaga agatttatct ggactattat caatgttttc
     2701 tcttgctata gaagaacaaa ttacattaga caaattgaaa atgttagatt atttatttca
     2761 tcctagtttt agccaaccct ataattttat tttaaaggca gtaacaggat taaacattga
     2821 aacataatcg ttactaaatg aaatcaagaa atatactgta ttactcaatt cattgaaaat
     2881 atttatcata atttttaata gaaaacacta ttaaagagaa aaaagagagg taaatcaaat
     2941 gaaaaaatct ttaaaacttg catcagcagt tatattaatg tcccaactcc ttatagcaac
     3001 acctttccat gtgttggcaa gcgaaaattc aacatacaaa attgcaaaag aaagaaccga
     3061 gacgactgaa atgagtaatt ccgatgtaac agcagagagt agtgaacttt tgacaacttc
     3121 atcaagtgaa agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag
     3181 tagtacgact tcaaatgccg agtcttcaac aacttcatca agtgaaagta gtacgacttc
     3241 aaatgccgag tcttcaacaa cttcgtcaag tgaaagtagt acgatttcaa gtaccgaatc
     3301 ttcaacaact tcaagtagta caagtgaaat aacttcttcg aaagtgacaa aaccaaacca
     3361 tatagaatct tcaaaaaagg aagcgccaaa aacggaagga caaacaaaaa ctttttcgac
     3421 accacagcaa atagaatcaa cagaatcttt ccaatcacaa ccaatactat ctggtaatga
     3481 agaaattcat tttgacaaaa atgaatctgt agaaaatttt attatgaaaa ttggtgagtc
     3541 tgcgagaaaa attggtcaga aatatgattt atatgcttct gttatgattg cacaagcgat
     3601 cttagaatct gccagtggtc aaagccagtt agctcaggca ccgaattaca atttatttgg
     3661 gataaaagga acttataatg gaaactttgt cattatggta acaaacgaag atttgggtaa
     3721 tggaacgctt tatacgaccc aatcaaaatt tagagtgtat gaaaactatg aggaaagctt
     3781 tgaagattat gccaaattat taactaaggg aattagtgga aataaagatt tttatgcagg
     3841 agcattaaaa gctaatagta aaacatatcg agaagcaact aaattcctta caggacggta
     3901 tgcaacagat acacaatact atttgaagct aaatgaatta attaaaacct acgatttaac
     3961 caactatgat aaagaagtta aaggtccaca aatgagtaag actggatacc aagtgcctat
     4021 tcataattat tcaataacca gtttgtttgg aaatcgtgga aatgaatttc accgaggact
     4081 tgatatggca gctacacagg gtgagccgat tcatgcagtt aaagctggta aggttgttaa
     4141 agcagagttt cactactctt ggggaaatta cgtggtgata caacacgaag atggatcaac
     4201 cgcgttatat gctcaccagc aacaatacct agtaaaaact ggagataatg ttttgcaagg
     4261 acaaataatt ggctatgtag gaagtactgg gaatagtact ggcccacacc ttcatttgga
     4321 aatttgcaaa gatagtagtc tttctcagag catgttagtt gatccgaaaa ccttattatt
     4381 cggagactaa aaaaggtaat caatctgaca aagtaacaag aaaatggttg gattcttaga
     4441 cctttgatca ggatattgag actggaattg atatctcgat tacgatgggt ttgacaaata
     4501 aaacaagtcc attatcgaat atcgagtgta ttattgccgt ctttatgctc atattttgag
     4561 caggtttcac tagatgaaaa caatttatct atcttgagga tttcgtgtac gtatcgttca
     4621 gtcttgtatt gtaaattagt cataaaatca gactaagaaa aattggaaat atttttttct
     4681 gatttattaa aagttaattg aaattatatg gtacttcttt cttgggaggg taggtgcagt
     4741 gaaggaaaga tatggtcacc ttactgttgt tgaggagatt actcataatg gatataaaaa
     4801 atatcgttgc ctatgtgact gtgggaatga aacgattgta tatcgatcaa acttactatc
     4861 aggtagaacc aaaagttgtg gatgtggaaa ttataaaaat aggcataaat ataaaaattt
     4921 gacaaataaa acttttggta agttagttgc aatcaagcca actaaactgc gaagtgaagg
     4981 aactgttgtt tgggaatgtc aatgtctgtg tgggagaaaa aagttagtat cggcaaagcg
     5041 acttctaaaa ggtgatgtaa aaagctgtgg atgtttgaaa tcaaaaaagc atcacattga
     5101 aaataaaaaa ttcggattac ttactgcaaa gtatcctttg gacaataact gttcatggaa
     5161 aacaaaatgg gtttgtcatt gtgagtgtgg cagtatgtgt gagataagtt atagtaactt
     5221 attttttgga catactcaaa gttgtggatg tttaaaaagg caagaaacaa ggacatgtgt
     5281 tgaagggaca gtaatagagt gtctaacgtc aaaattaccg aaaaataata caagtggtgt
     5341 taagggagtt tatcgttcta aagagaaata gattgcgtat ataactttaa aaaaaaagag
     5401 gtactattta ggatcgtatg attctattaa gagtgcagct caagctagaa aagatgctga
     5461 aaataaattg tttcaacctt tgattgacaa atatcgaggt aatcattaat tattgtggat
     5521 aataattaac agtgatatat aaacatctat aatatattat tcatactatt gatatttagt
     5581 ataatcctat ggaattgaat atcatagcaa tgtatttgaa aagcaaattg gaaaacaaat
     5641 aggtgcaaca ccataaatat gaaaaaattg ggaaaaagta gaatggaaat ttgcttacgt
     5701 ctcacgagat agctcacact attaatctga agaacaatga agtcgacttt tagaaattgc
     5761 tagaagaaag taaagcgaat tatcactagt tactatagag taagttcaaa taaagcatct
     5821 ccaacttaca tggttacatg aggatatcaa atcgaaatta ctcaagcttc ctatggctag
     5881 agtcgcgaga agttgttggg cagtagacat aggttatatt gttcttagct acacgatcat
     5941 tagaaaggaa atcctattat gtatagttat caagaagatg caaaagtcca aaaaaatcgc
     6001 tggtggattt tgattgctat atctatgttt accttcatgt ccacattgga tggcagtatt
     6061 gtcaatattg cactacctac catctcagat gatatgggca taccaatgaa tcaagcggaa
     6121 tgggtagtgt ccgtttatct gatgattgtc tgttcttgtt tgttgctgtt tggtaaaatt
     6181 ggcgatagcc gtgggaaaat caaagtattt cggattggta cgctgatctt tgtcttggga
     6241 tcgctgttgt gtgggttcaa tcattcgcta ggtattttat tgttcgcacg ggtggtacaa
     6301 gccattggtg ctagtatgac tatggctaca aatgccggaa tcatcacgga agtctttccc
     6361 atacatgaac gtggtcgggc attgggagca ataggtgcgt ttgtttcatt aggctcgatt
     6421 gctggaccag gtcttggcgg cttgattttg tcccaatttt cttgggcgta cattttttgg
     6481 atcaatgtac cggtgggaat cgtaacgatg ttgattgctg aaaaattctt accaaaagat
     6541 atcaccatga gcggccatac aatcgatgga gggggctttg ctgccattgc ggctacgatt
     6601 atcagctttt ttggagcaat ctttttgggg caagaaagtg gctttttcca tccagtcccc
     6661 cttgtactgt tcgtaatttc agtgattgcc tttgtcagct ttttaaaagt tgaaaagaaa
     6721 gcagcctctc cattgatttc tttcggtatt tttaagaaca agatttttac catgagcttg
     6781 attacagcct tgttgatttt ttcttccaac ttctttgtca atgtagtgat tcctttttat
     6841 ctgcaaaatg ctcgcgggtt gagtcctagc tatgctgggc tgttgatgat ggtctttcct
     6901 ttgatgatgg tcgtcggggc gcctttaagc ggctacctga ccgaccgggt tggcccggag
     6961 attttggtat tagtaggctt gggcttattg tcggtaaccc aggtcatgta tatgtttttg
     7021 tcagtggact ctcctttgtg gctatacatt gtcgctactg ccatcatggg tgccggcaat
     7081 gccttgttcc agtcccccaa taatacgatg gttatgagct cggtcactat cgaaaacttg
     7141 ggactggcag gctcgatgaa ttcctttgcc cgaaatttgg ggatggtcag cgggatttcg
     7201 ctttctacga cggtcttata tcaagcgatg tctgctaaga tgggccagca agtaactaac
     7261 tatatcgcaa atcggccgga tgtcttttta tatggaatga agattacctt tttaggcagc
     7321 tttgttttgt gttttgttgc ctttttcctg actatctggc gtatcaaaaa acaaggaggt
     7381 attcagaaaa atgtctgaaa aaaccactgt tgaagaattt ttggaggcct gcgtatgggt
     7441 gagtgggatt tccatggatg atggaagatg aagtgaactt ctaaaagccg aaaaactcaa
     7501 gtattatctt caaaatacta gtgctcaatg agatgcttga tttgatcaca agaagactgc
     7561 gaaagccgct ggcaaccgca agctaggtat cccaccgatg gactacgttt tagaggctac
     7621 tggttatgaa tacggagcca acgctctcat ctgcataaga atacatcacc cagattatgt
     7681 aaagattttc gatacttctg tcaaaaaatc attaagatag ataaatgatt tccgtctagt
     7741 caaattggtt cagaatgtag acacacagat ggtaagaagt cactcacaat tcgagaatgg
     7801 acttgtccta tttgccgtat acatcataat cgaaacgtca acgatagtat caatattttg
     7861 accaagggct atgaatacaa gcattgcctt agaaaacatc aagaacctta ggaacaacgg
     7921 ggatagcttg gttaataaga gataccgctg tttaggcttg caagtatact cttttatcaa
     7981 gaattccgtg gctttagtca tgagaggctt aatactatga aaattttctt tagcattttt
     8041 gatttaggtt ccttttctaa ttataataag atgaccacta cagtaaccta ctgaaatata
     8101 taaacagcca ctgtagtacc attttcaaag tagtctatga gaggagaatc ttcattctta
     8161 tgaacgctgg aattgcggat atgaatttaa taaaaaaaac ccttaatgat tttacttcca
     8221 attcaatatc aaaaggtact ggtatcaatt taagcacaat aaaaaagctg aaaagtggtg
     8281 aaagatcagt ggaaaagctg aatcttttag atgctataaa aataacggaa tttgctatga
     8341 aaaatggaaa agctgagatt gaaatatgga gataattatc gtctagtgaa ttacttgcca
     8401 cttagctgaa tttaacgatc cttggtgctt gaagggggag attcttggga atagagcata
     8461 cttgcaagcc tatgtcttta ctaacaacga tatctcttat taaccaagct atccccgtag
     8521 ttctaaggct tttactttta tctaagcaat aatcgtattc aaaggaaatt tttgaccctt
     8581 agaaagtctc attttatcag acgtgagctt aggcattgct gaattttgtt aattcggttt
     8641 taggttgtgc tttgagcata atatgaaagt attctttatc acgatttcat tcatctaagg
     8701 taatgtgatg tgattctgaa attctttcaa aggcagtttt tgcataagct gacattacat
     8761 catcaatgac atgtttacaa tgtttcacta ctaaaataag ggggtaacca agaagaaaaa
     8821 ctgaatgatt atttgtatct aattccatgg tatatcaacc atttcctata tagactgatt
     8881 atgacatgga ttaaaataaa aaggcactat gtcttaattg tcggttttcg agttaccgaa
     8941 tggcattcat cacccaccta tagaggatgg gcgacttctg cctgaaataa gttacactac
     9001 ttataacttc taataaaatt agattgagtc attattattt aacatcaacg attttccagt
     9061 ttccattttt ttgtaatact aatttgtact gaaagtagct agtggttttt gatacattat
     9121 caaggtattt tatctttgta ctcactagaa aattttttcc gttttgtaga taaactggat
     9181 tctctaactc cgaaaacttt aagtcaatat tgattggtcg catcgcacca ttttcaacat
     9241 aatactccaa ttctttttca gaagctgatg gataaagttt aaaaaatgtt tctaaaaaat
     9301 cggtgatttc ttccgttgtt ttggcatcaa tttctgaatc gttttctact tgttttgctt
     9361 catattttgc cttgtctggc tttccaatca ttgttggcaa actagttact aaatgattcc
     9421 tgttttcatc ttcaaagatt gtcactcgat atgcagaaga aattgattgc gtcttttttc
     9481 cctctgtaat atgttgctcc actaaaaatg aaacgacaaa ttcttccgag tgtttttcaa
     9541 catctagaat ttttacagat tgaacttcgg aactcgtggg aatatctgct cgaaccatat
     9601 cttggattaa agcgagaccg ttttctgtaa ggtactgttc gagattactc attctctttt
     9661 caattgcttc tttgttattt ttccatgaaa aatattcttt cgcaaaatcc tctgtaaaac
     9721 tttcaacgcc agaagtattc actaccttct cttttataac cactttttca tgagttgtat
     9781 gttgatcaat ggcggtgaaa tttttataaa cgccaaaagc aagactacaa gacaataaaa
     9841 gccaaagggc aagcaccatt tttcgatgct tacccacgga tacaactcgt tcttttttct
     9901 tttttgggct cttttccttt tgattccgtt caatttttat cttcattctt tttcctcttt
     9961 tctcttattt gtatcggcca gctccgataa gatgctgccg ccaatagttt tccgttagat
    10021 tcgtccaccc cactggatct cctgcatgaa acatgcgatt ctcccctgcg taaatgccca
    10081 catgggtcac ataagtaccg gcatcatagg ttgaatgaaa gaaaactaaa tcacctggtt
    10141 tggcatcttt tagatcaata tgagtcatgg catcatattg atcctgagcc acccgaggaa
    10201 gtttcaggtc aactttggca taacaccatt gggtcaagcc actacaatca aaccccgttt
    10261 ctggacttga gccaccaaac acataggccg ttccttcata cttaaatgcc tcatcaaaaa
    10321 tttcttggac tattttgtca tcaaactgag ttgtagttag gtattgcttc actaataaac
    10381 agtaaaacat attgccataa ttataccgcc acccaccatt ttccttaatc gcgattgggt
    10441 tggggtaatc gactttttga ccacctgatt tttcctttga aaaagattca gctaaagcaa
    10501 aactatgctt cttcccgttt ttcccgacat gatctagata cccgccacca tagttatagg
    10561 cctgaataat actatcttga tcacacccta gttctttgga acgcttaacc aaagacgcaa
    10621 aatagagaca gccttgtttg atggatgcct cggtacttaa tgaattaggt ggtaatccgg
    10681 ctgactcaga actttgcata acgtcttcca ttgtgccacc agattccact tgaataatcg
    10741 ccaaaagaat tggaatatac tctgttatat ccgattcttt ggcatacttt tccaccattg
    10801 cacggtgttt caatacttct tctgagacac tagttccgcc aggagaagtg ccagaactac
    10861 catttccctc actgtcttca ccaaacaaca aacccacaca catcaaaata cccataaaag
    10921 agatggtcag aaaagttaat gaaataacac ccactttctt caagttcatt ttttctcacc
    10981 tgaccttgat tttggacgat ggttcattct tttttgtact ttttgattgg tctgctttgg
    11041 cagagtggac ctctgtttca cttgttggaa agaatttttc ttacttgctg attggctttt
    11101 cccttttgaa atgatttctt gactagttag ttttcgtttg atttcgggat tcagatttgc
    11161 tctcacaatc cgctttggtg cttctttttt cagcatcata tctttctgag gttgagctgc
    11221 tttatctatt ggaatattcg cccttctttt tccctgacga tttgttgctt cactcagtat
    11281 cttgcgtttt tcgtccattt tcgcatttct tcgttgtcgt tgtttttctc gcaaatccac
    11341 tcgatccgct ttttcttgga acagtccgcg cttgaattct tctggtgctt ttttcgactt
    11401 ttcgattcct ttatggagat tgtatttcaa attcgttggc gtattcctta cctgatcctt
    11461 tacttgttta gtcttatctt tcaccaaagc ttgggtatcc aaaactttgc cgatttttct
    11521 cccagccaaa ttcattcggg attgtttctt attatttgaa actggattct ccttagttaa
    11581 gtcagatagt atttcgtgat cgttcggtaa tcgtgaattc tttcgtagag aagaaccgga
    11641 tggcttaaat ttggattttg attgttcctt tgccaactta tttccgactg ctgcaccacc
    11701 accaaaagcc aaagctcgtc ccacatttcg attgagttgc cgcaattttc gattcattaa
    11761 catttgcggt tttcgcatga cgcgtcttcc cattgactgg ctatcattac tctggaggtt
    11821 gaacatactc ataatgtcgc ccaatttgaa atagataccg gcaaaggtca caatttgcag
    11881 aaaagcaacc atgaaaaatg gataggttgc cgaaatattg aaaaacatcg tggagatgct
    11941 aaatgcagtc gtaatcacca aagtaacacc cgctcgcatc ataatcgtat tgaataaacg
    12001 aataatcgcc ttctttccta aactttcata agtcggcaac atggacaata agaaactaat
    12061 tggcagaaac atcgcaaaga taatgaagag aatttgcgag aacaacataa tccctgttag
    12121 aagaaaaaca aagaaggaaa tgccaatatt gaacaatacc aaaaagacca cggtccctaa
    12181 acggttcatg gtcttggtaa tactgagatt cgcattgtca ttgtcttcaa tctctgcctt
    12241 gaccgcttct tcccgatctt tgcctttgtt ttcatctgga ctcacagata gaatcttgtt
    12301 gacccggtct tcaccaattt cttctatgtt ggaatcatca aattgcagta gtaaccaagg
    12361 ttgttggact tgaatcgaaa acaaactatc ccgaatcaaa tccacactat cttttccttg
    12421 gctttctgaa ttgggtacaa cgatctcggt tccaagggta agggccgctt cactaacatc
    12481 tgagctaaag tcattgattt ttgtgatata ggtaggcgca taagcaatga acgaacccga
    12541 tagtagaaaa atcatcacaa aattaatcac ggctcgaact gctttcgtgg tttctcgttt
    12601 taataatccg gtatacgcca catacacccc cagagctaaa atcatcaata atagaaaccc
    12661 aaaataaaat ccggaggtct ggagtccatt ttcagtaata ccagccaatg tttggatatt
    12721 ctttccaata ctttcagccg tatccgaaat gaaatctaat ttgtaagcct cttgaaccac
    12781 ataacccgtg gcgttcgaca aatacaaact caccgtccaa atgaaattag taatggcata
    12841 aagaccatac atcacgcttt tccctaagcc atcaccccaa ttccaaggca accaatccca
    12901 agagctatcc acaaagaaat ctagttgata gttgttcagt gaatatttgg aatatagatt
    12961 tcctgcttca actgtatcat caactagccc tgtcgcctcg gctaccgtac caactaaact
    13021 caatagaact atcagagtga gtatgctaat aaaaacgatt cccacaatcc gaaaaagttt
    13081 ctttttcatc ccctcactcc attctctctt gaacaggtgg acgggtatca aaggcatgga
    13141 agagttcttc aaagactgga tgtacctgaa ccacgcctac ccgaccatac aaatcctgca
    13201 tcaagcattg gccattttcc aaatctcgta ggcgtttttg attgctttca tcttctcgat
    13261 ccaagccaaa gaattccaag gttttcttga tttcatgaat atcggtactg cgaaacgcaa
    13321 acttcaaccc gatattattc ttcaagcgtt catccagtaa atcatcacta ttttgcgtga
    13381 caaagtaaac cccggcattc atagcccgcc cagcacgaac gagcttattc gataatgttt
    13441 tcccttgcgc tacttgaaga aaactccacg cctcatctaa gtctacaatt ttgaagattt
    13501 ctcgattact atggataaag tccaaggcaa aagtggaaat gacgattagc atggaaacac
    13561 taagcaattc catggtggta tattcttcaa aagtggtttc tgcatctggt aaaaccaaat
    13621 ccgctacttg aatgatattt aactgcctat ctaaactaat cgactgggta atcgaaccat
    13681 ctgagaaaag caaatgggca aaatcataat cggtaaagga ctcgatgtga tccgcgatat
    13741 tttccgcaac tggtgagcct tcttttcgaa gctcattaat tacttgtaat aatccccgtt
    13801 gattgctttg ggtaaccgtc cgaatggcct tacgcaacac tggaaacttc tccccatctc
    13861 gactagaaat tcctgttaaa aaggtcagaa tatcaattgc tagactttca gaatccttct
    13921 ttttctccat aatcacataa ggatcaagta atccttgatt ttttggctta ctagttaagt
    13981 tgagaatatt gatttcatcc gcaatttctg gcaaggtttc tttccagcca ccccgttccc
    14041 cttttggatc gacaatcaca gcttgtccac caaaaaggac ggcgtaataa actaagagat
    14101 tgttactaaa ggactttccg ccaccgagtg aaccgaggaa tgccgctgct agcgcattgg
    14161 taactgaacc cttgacccct tgtgaggcca atgccggttt caggtaaaca ttgcgccccg
    14221 tatctacgtt ataaccaaag taaatgcctt ccaactcccc taacatctga gtggcgccaa
    14281 aaccaagtcc ggcgagaaaa tcagacgtaa catactggat ataatcattc atgtatcgct
    14341 tactagaagg taagaattct tcatgtagcc ccatcatatc gccaaaaggc cggactagtt
    14401 ttacattggt atcatcataa aaatctagca cttcatcaca ccggcgcttt agttcatcta
    14461 ctgattcggc acttactcga accacatagc tcagtttgta catggattct ttggactgat
    14521 ccaaggtagt ttccaattca tctacggaat ccaatgcatc taagacattg gaatttgttt
    14581 cattgtcaga ttgataggca tggttgtcca agtcttttaa ttcctttttc ttattgcgaa
    14641 cggtagcaag tgcctttttg ttcgtcacaa tctccacatt catactagtg tcaattggga
    14701 atgtaaattg ttgttgctgg taatagaaaa gttctgagga gggaaactcc atttccccca
    14761 caattgtatt aattgttaag tacgctacat atgattcatg attctcatgt tccatgcata
    14821 agtaccgggg cttttcttca atcaaacaac gactgggacg taataagtcg taacgtttca
    14881 ccaaagtttc cgacttcagc tttttctttg gtagctgaaa ttcatattct tcaaatggtg
    14941 tacccttttc gccatagata tgttcaatca aatacatcag atcactcggt gtgactcgtc
    15001 tgactttaaa tcttcgagcc aatttacttt ccattagttt ttcaagcttg gtatatcggc
    15061 gaatttcttc attggataag gaaacaaaat cgcccatcaa atgatgattc actccataga
    15121 caaattcttg aaagccggag aagaaagatt tcttgagatt ctttagattg acctcttcat
    15181 ctgtcgcaat cagtttgaag ccaataaaaa agcgataatc aatctgacta tcgccaatca
    15241 tggatactaa tgcctctgtc tgtaaatcaa ttcgttgttt cgctacttct gttaatcgcc
    15301 cggtaatttc cttttttgat cgttcttgcg tggcacgaat actactttct gtggcaattt
    15361 gtaaggcatg aatcttgcct tcacgatttt gcgcaatcaa ttgacgaaag ttatcgtgga
    15421 cttgatattt ctgttcaggt gacaaaaagg agtaattgta aggtattaac tcatagtagg
    15481 caaaacattc tccctcttga ttaaagacaa gattattttc aatatactta atcgggtaca
    15541 tagttcacac tccttaccag agtaatgggt tcgtcccatc gtttcttctg ttctttgact
    15601 ttctttcctg cgaaagtgac cttaaatcgt aagtgatagc taataaaaga tcgtaaaaat
    15661 cccaatggtt tcttaccgtc aaacgttttt tgcgacatga accaagtaac tgccaccggt
    15721 atacctaagt attttaaaaa ggccccctct atcatagata gtggaggcac atgggcaaat
    15781 acgatgacaa acagcaagga taaaacaaac caagccatct ggttaaaagt cacaggaaaa
    15841 ggcagttgaa agtcattaat tgcataaatg accttttcaa ctgcccaaat actggtgtag
    15901 ctttttattt tcttcatcaa atcattcctt taaaaagaag gatagtttag aaatcaacta
    15961 cccttcaata attttgttaa tatggatact caaaaacacc tcgactcgtt accaaataat
    16021 tgcctccaat ctccaagtct ctaccacacg catcaaaatc aatatacgtc ttcaagtgtt
    16081 ctggtacttc tccaaaatca ccagcttcta caatcaagaa atatgctaca tcactcatat
    16141 catcgcaatc tggataatag ataatatcat ccacatgctc taccatctcc tcaaaagaat
    16201 tgaagaaagc atgttgaatc tcagaagcaa cttcaccaac tggcgttcct tcaagttcct
    16261 ccgctaagcc acacaaccga ttaatttcct caattggcgt atactcatca atctcaaagg
    16321 gaagttcata atcatgaatt gcatattcct cgtattcgtc atttaatcca atccgttctt
    16381 tcacttcatc aaaatcgacg ggtggggtaa accatgcccc caccaactca ccttcattgt
    16441 attttccaag gttcgcaata taaacacgca tctgttccat cgtgcttccc cctcaggcac
    16501 cgacaattcg gttaaacaaa ctgagtaaga catccttcac cccagaagca ttaaagacca
    16561 atccgaccgc aatcaaagca atcaccaaga agccaatcag tttggaaaac tcgcgtttaa
    16621 atcctaaaaa caaaccaatg accacaatcg ccagtaagac taaggattg
//
LOCUS       DAFEOM010000059        16527 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.062, whole genome shotgun sequence.
ACCESSION   DAFEOM010000059
VERSION     DAFEOM010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.062"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(141..416)
                     /locus_tag="LP524_002067"
     CDS             complement(141..416)
                     /locus_tag="LP524_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBL2235094.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(659..1969)
                     /gene="der"
                     /locus_tag="LP524_002068"
     CDS             complement(659..1969)
                     /gene="der"
                     /locus_tag="LP524_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBL2235095.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(2151..3380)
                     /gene="rpsA"
                     /locus_tag="LP524_002069"
     CDS             complement(2151..3380)
                     /gene="rpsA"
                     /locus_tag="LP524_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBL2235096.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(3515..4195)
                     /locus_tag="LP524_002070"
     CDS             complement(3515..4195)
                     /locus_tag="LP524_002070"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBL2235097.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(4244..4879)
                     /locus_tag="LP524_002071"
     CDS             complement(4244..4879)
                     /locus_tag="LP524_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL2235098.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(4945..6348)
                     /locus_tag="LP524_002072"
     CDS             complement(4945..6348)
                     /locus_tag="LP524_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBL2235099.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(6335..7366)
                     /locus_tag="LP524_002073"
     CDS             complement(6335..7366)
                     /locus_tag="LP524_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2235100.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            7397..7618
                     /locus_tag="LP524_002074"
     CDS             7397..7618
                     /locus_tag="LP524_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBL2235101.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(7783..8520)
                     /locus_tag="LP524_002075"
     CDS             complement(7783..8520)
                     /locus_tag="LP524_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HBL2235102.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(8673..10013)
                     /locus_tag="LP524_002076"
     CDS             complement(8673..10013)
                     /locus_tag="LP524_002076"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBL2235103.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(10250..11773)
                     /locus_tag="LP524_002077"
     CDS             complement(10250..11773)
                     /locus_tag="LP524_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBL2235104.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(12151..12762)
                     /locus_tag="LP524_002078"
     CDS             complement(12151..12762)
                     /locus_tag="LP524_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL2235105.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(12885..13010)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(13092..13808)
                     /locus_tag="LP524_002079"
     CDS             complement(13092..13808)
                     /locus_tag="LP524_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBL2235106.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(13814..14383)
                     /gene="scpB"
                     /locus_tag="LP524_002080"
     CDS             complement(13814..14383)
                     /gene="scpB"
                     /locus_tag="LP524_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBL2235107.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(14367..15164)
                     /locus_tag="LP524_002081"
     CDS             complement(14367..15164)
                     /locus_tag="LP524_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBL2235108.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(15134..15505)
                     /locus_tag="LP524_002082"
     CDS             complement(15134..15505)
                     /locus_tag="LP524_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBL2235109.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(15524..16411)
                     /gene="xerD"
                     /locus_tag="LP524_002083"
     CDS             complement(15524..16411)
                     /gene="xerD"
                     /locus_tag="LP524_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBL2235110.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         4645 a         3576 c         2798 g         5508 t
ORIGIN      
        1 aaaaattaaa taaaatacat gtgttttcta tgtatgacat acggattcgg aatctctgtc
       61 tcttatttat tccttttctg ctaggaaata aaaagaaaag cacttgtagc cctagagcca
      121 caagcgcttt ttcaactgtc ttatttaaca gcgtctttta atgctttacc tggtttgaat
      181 gcaggtactt tgctagctgg aatttcgatt tcttcaccag tttgtgggtt acgacctttg
      241 cgagctgcgc gttcgcgaac ttcaaagtta ccaaaaccga ttaattgaac tttttcgccg
      301 ttagctaaag catcttgaat agttgaaaat acagcatcta ctgctgcagt tgcatctttt
      361 ttagttaaat cagtagctga agcaactttt tcgattaatt ctgctttgtt tgccatggat
      421 agattcacct cctccacaag agatatgata gaaatatcat ttagttaaaa ctttctgagt
      481 ggtccagaag tttaaaaaga aatattgatt aacttcaata ttctgttatc acgatatcat
      541 atgaaccgct taatagcaag gtttttacca attttcacat gcttttttta gtcatagaaa
      601 cgtaacaaac gaaactgtct ttttcttttg aaaaaaaaac gtcgatatgg taaaatgcct
      661 attttctcct acgagggata attcggatgg acgtcccttc aaaagtaaat gcttttctga
      721 tttgattttc taagaaacgt tcataagaaa aatgcatcaa ttcctcttca ttgacaaaaa
      781 cgacaaatgt aggtggtttg acagcgactt gtgtagcata aaaaatcttc aggcgttttc
      841 ctttatctgt tggtgttgga ttgattgcta cagcatccat aatgatatca ttcaacacag
      901 ccgaagagat acgaagattc tggttcatac ttacttgttc gatcatttca ggcagacgtt
      961 ccaaccgttg tttagtcact gcagaaacaa aaacgattgg agcgtaatca agataccgga
     1021 attcatcacg aatctcttgt tcgaaatcac gcattgtatt ggtttctttc tttacaagat
     1081 cccatttatt cacgacgata ataattccac gaccagcttc atgggcgtaa cccgctactc
     1141 gcttgtcttg ttcccgaatc ccttcttcag cattcagtac catcaaaacg atatcagaac
     1201 ggtcaatggc acgcattgca cgcatcacgc tgtatttttc agtattttcg tatacttttc
     1261 cgcgtttacg cataccagct gtatctatca ttaagaattt ctgaccactt tcagaaacga
     1321 agtgggtgtc gattgcgtct cgggtcgttc cttcaatatc cgaaacaatt acacgttctt
     1381 ctcctaaaat cgcattgatc aaagaggatt taccaacatt tgggcgacca atcaaactaa
     1441 acttaatcgt gtcgtcgtcc tcttcttcag tctcttcaga gaaatacttc actgcttcat
     1501 ctaaaatatc accaatccct agtccatggc ttcctgaaac aggataagga tcgcctagcc
     1561 ctaaggaata aaattcatag atatcgtttc tcatttctgg attatctact ttattgactg
     1621 ctaaaatcac tggtttatta cttctgtata aaattttcgc aaccatctca tctgcgtctg
     1681 tcacgccttc gcgtccgctg actacacaaa tgattacatc tgcttcttca atcgcgattt
     1741 ctgcttgatg cttgatctga tccataaaag gttcatcgcc aagatcaatt ccgcctgtgt
     1801 cgatcacgct gaattctcga cctaaccatt caccttttgc ataaatacga tcacgagtta
     1861 ctccaggagt atcttcgaca atcgagatac gctcaccagc gatgcggtta aaaatcgtag
     1921 acttaccgac gttcgggcgg ccaacgattg caattgttgg atttgccata ttatttcctc
     1981 ctcacaagtt tccaatctca cttagtttaa cgaatgcatt gccaactttc aagcatagat
     2041 tgtcaattaa acgaagtatt tttattcctt attgaaatac taatcgcttt tcaaaagaaa
     2101 aactgagaca aaagcagctg ctttcatctc agtcacagat acaaaatcaa ttatttttca
     2161 gaatcatcgg ttgctttttc ttctacttct tctttcaacg catcacctaa aatgtctccc
     2221 atagtaaatc cagtattttc ttctggtaat tcataagact caacattttt tggttcttct
     2281 tctgaagctg gtttttcttc taaagctttg atactcaatg caatacgatg ttcttctgga
     2341 tggatctcta atactttcac tttcacttcg tcaccttcat gaagcacttc atgtggtgtc
     2401 gcaatatgtt tatgtgaaat ttgagagata tggactagcc cttctactcc agggaacact
     2461 tcaacaaagg caccgaagct tgtcaaacgt ttaactgttc cgtctaatac tgtgccaact
     2521 ggagcttttg tatcgatttc tgaccaaggt ccaggctgtg tgtctttgat tgacaaggaa
     2581 acacgttctt gttcaggatt gatagacaac actttgacat ttacatcatc attgacagcc
     2641 aatacatcac ttggttttgc tacatgacta tgtgaaatct ctgagacatg gactagaccg
     2701 tcaattccgc ctaggtcgac aaatgcgcca aaatcagtaa gacgtgcaac acgtccactg
     2761 agaacatctc cctcatggat agttgaaaga atttcttttt tcttttcttc tctttcgttt
     2821 tccactaccg ctttatgcga taagatcaaa cgattttctg atggttcgat ttcgatgatt
     2881 ttaaagctca atgttttgcc tttatagtct gaaaagtctg caacaaaatg atcttccacc
     2941 attgatgcag ggacaaaacc acgtacaccg acgtcaacga ctaaaccgcc tttgacaaca
     3001 tttgtaacag gtgcttcgat gaccttgccc tcttggaaat ctctttcgat atcttcccaa
     3061 acttttttag catctaaacg gcgttttgaa agtaggtagc tgccgttttc tttatctttg
     3121 ccgatcgtac tgataacgac taaatctaat acatcaccga cttttactac ttcgctgata
     3181 tcttctaccg gcaaagttga taattctttt gccggaacga ctccttcaac tccagcccct
     3241 tcgattccga caatcacttg tttgtcttca atgactaaaa cttcgccctt aacaacatcg
     3301 ccaactttta cttcttgtac gcttttcatt gcgtcttcca ttgactggtt gttctcttga
     3361 ttttgatcaa attctgtcat aacacttgtc ctcctaacca acattctgcc gtaagtacgg
     3421 tactgttttt agtttaattg attattcaaa aaaaataaag cgatttcgtt cgttttcctt
     3481 gaaaaaacaa ttgtttttca ttttttttct taatttaaaa aagaagataa ccttttttca
     3541 aaacaacagc ttttattgct gcaacgactt ctggaatcga tttgccagtc gtatcgatac
     3601 gaacagcatc ctctgcttgc ttcagcggag agacgtccct tgttgaatca taatggtctc
     3661 tttcctgtat ctctttcgtc agtgtttcaa gatcagtcgg aattcctttt tcttggttct
     3721 ctttgaaacg tctttctgca cgctcactta cgcttgctac taaaaagatt ttcacttctg
     3781 cattcggtaa tacagctgtc ccgatgtctc ttccgtccat gacaacgcca ccgcgttccc
     3841 ctatttttcg ttggacagca actaattctt ctctgacttt tccatgtgca gagacttcag
     3901 aaacagcttt tgtgacatct ggttgacgga tttctaaagt cacatctttt gatgctatga
     3961 atacatgctg tccatcttct tgttgcgaaa aagagatagg atactttttg atcagcgaaa
     4021 ctagcccttc ttcatctgaa aaagatacat tgttcgtaat agctaaataa gttactgcgc
     4081 gatacatggc accggtatct gtataaatat aattcaattc ttttgctaat atcttggcaa
     4141 cggtactttt cccagatgaa gcaggcccgt cgattgcaat actgatttgt gtcatgatat
     4201 aactcctttt tctcagaaaa aaagccgcaa agcgcggcta ttattattta attcttaatt
     4261 cttgaccagg gtaaagcata aagttgtttg gatctaagcc attcaattca aacaattgat
     4321 cagtggtgat ccctgcacgc tcagccactt gttttggacc ttcgcctgct tgaacagtcg
     4381 ttgttgaacc ggctgcagct gatgaagaag gtgttgtttc tgctgcattg ctgcttgaat
     4441 cccctgcttg ataagttgcc tcacttgagg aagtcggtgt tgtctctgct gcaatactgc
     4501 tttctgacgt tacacctgta tcttgagaca cagtagattc ttccgcgatt gtactagatt
     4561 ctactgttga agtctcagtt gactcctctt ttgttgtact tgtagaactt tctactactg
     4621 atgtcgaagt ttcagaagaa gttgctgctg ttgcattgtt gcttgatcca tttctgatcc
     4681 agaaataagc acctgctgga atagcgatac ataagaccag taaaatcaaa aatagcgaaa
     4741 gaaataatgt atttcctttc ttttgttgtc tctgagaact tcttgatgaa acttcatcat
     4801 ctgtatcgta gattggttgt tcccatggtt cttgtgtttc gttattttct gtagattgat
     4861 gtctatcttt tctgctcact gttttacctc ctaaactcta tcgtgtgtat tataccatag
     4921 gaaaaagtgt cttgtcctaa aaaatcaatg ttccatgttt tttttaaata aatttagtaa
     4981 aatcgtttcc caatgaagtt gttcagtctt agaaaaaagc ttcctctttg gcagaagttc
     5041 tgcttttgct ccatcaatat cacaacaatt ctgcggcttt tcggagagtt tttcgccaaa
     5101 ataagctaag atgaattttc tgcggcagtt ttcttcatta atatagcgaa gcatctgctg
     5161 caggcggaat tctttttctt tttcctgacg tttgagccgt tccacccaat tttctggttt
     5221 ttcagactgc tggatcagct ccatccattt tttttgcagc tcatcaaagg gagcttgctt
     5281 tgtggcatat tccaaataaa gctcaaaact ttgtcgctgt tctctagaaa gttgattaaa
     5341 gaaataatgg atacgttcat ccccactttg ataaaaaaga attgctgcac tttcttcctg
     5401 atctctgcct gctcgaccga tttcttgtac atagttttct agactgtctg gcaaatcata
     5461 atggaccaca taacggatat cagatttatc gatccccata ccaaatgcat tcgtagcaat
     5521 gagaaactgc agctgatttt ttacaaactg ctgttgaagt tgtcttcttt gagctgcatc
     5581 aagaccaccg tgatagtatc caactgtaaa acgttccctg aataaatgat acaatcgttc
     5641 gacttctttt tttgttgcac aataaatgat tgccgctcct tgtgctcttt ccatgaagtg
     5701 ttcaagttct tgttcttttt gctgcgtttt ccgaacaaaa agcgagatat tttgccgatt
     5761 gactgattgt ctgatctctt ttggtggtcg atcaaatagt acatgacgta tatctcttgc
     5821 tactaaatct gttgctgtcg ccgttagtgc cagtgtgacc gggtttccta aaagctttcg
     5881 gatttttcct aactgttgat attctggacg gaaatcaact ccccattgag acacgcaatg
     5941 agcctcatct actacatata acgcaatatt ttgttttttc agatgctcta aaagagaagg
     6001 attagtcaac atttcgggac tcaaaaacaa aaatttatat tgcgatagat gacggaggac
     6061 gtatcttctt tcagattgcg aaagtaaact attgaaagca gctactcgtt tctcccctcg
     6121 tttttgtaat tgcgtcactt gatcttccat tagagaaagc agcggtgaca cgatgatgac
     6181 cagaccttcc attaaatacc ccgtcaattg atagcaaagg ctttttcccg ttcctgtagg
     6241 caaaatagcg agagtatctt ctccatcaag caacgcctga ataatttctt cttggccggg
     6301 acgaaatgaa tcaaatccaa aatatttttt taattcattt attaacattc tgtatcgctt
     6361 cctttctcaa tagatagatt tgatacaaac gaaattctcc ataatctaat tgtccatcta
     6421 cattccattc gctaaagcgc cactctcgca cggaatcctt atttggttcc aaacgttcga
     6481 ttgtttctgc agagagaatc cactcgtatg gaaaatcatc aaaaaacaag gcccactcaa
     6541 taagatgatc tgttactgtt cctcttttta aatgacggat tgccatgatc tggtcaattg
     6601 tctgaccatt cataaacatc tgcttggtga tcatcatgct ttgattgaaa ttttgctgta
     6661 ataatgaatc gatcaaagcg tgccaaagcg aatctggccg tttttcgatt tgtgctaaca
     6721 aaaggtggat acatctgctt tgttgcagat aaacctctga ctcatcattg ttttttgcca
     6781 gctgataagg aagcagccct gttcgctcat ttccagataa ttggttagct aaaaaatctg
     6841 ctgcttcttc tggtatctcc gaaaacagct gcaccaaact ctcatacgca gtatcaatca
     6901 aaatttcacg tggcaattgg ctttcactaa gccatttttt tagttggaaa gtataaaaag
     6961 gacttgtctc tgctggaaga tagtcttgaa tacccgttgc cagattagaa attacttgaa
     7021 cagcaaattt aatgagccgc caactagttt cccctgttct gccatagcgg tcaaaacgca
     7081 aaccagtatg ttctatttga tgttcggaca acatatcagc ccctgcaggc gttagctttg
     7141 cttcattttc atttatttcg atccaacctt gctgcgcaat tttttgcaaa atttgataaa
     7201 acttatcctg cttcaattct ggaaaactgt tatggataaa caaaagttcg tgggtaaatc
     7261 cataaataag tactgaagtc gttcgttttc ccacaaggag ttggtaaaga gaactaatcc
     7321 gcagcttgtg tcctgatgaa aatagcgata aaataaaaac aatcatatta ttctccttaa
     7381 gttaggatgg ttagaaatgt cactactatg taaacttgta cctgaacgct gtattgcatg
     7441 cggtctttgt caaatcgaag cgccagatat tttcgattat gatgaagaag gaatcgtttt
     7501 atttgcccaa gaaccagagg ctcatcaaca gttcgtgatg ccggaagaag aaacatatgt
     7561 aaaaaaagcg tatcaaaaat gtccagttcg agcgatttta ttagaaaata aaatataaat
     7621 ataaacgaaa ctttaaaaac gtacactaag ttctctaaac ttgaaaataa gccgggatct
     7681 gtttctcttg ttccgtgaaa tcccgactta ttttgaaaga ttcatttgct atctatagtt
     7741 aattgactag ttttttcacg gaccctatag gacccattta tattatttca actttttcag
     7801 ccagttttcc aatgtattga cgatcacagt ttgctggtct gcgttagaaa tttctgcctc
     7861 tccatctcct ttttgctcac catagctgcc aaaacctcca tgatttccgc cattgattga
     7921 tacatactct gttctttccg gtaaatactg cttagcttct tgataagctt cctgattcaa
     7981 tactttatct tttgtcgccg tgatcgataa aacaggaaca gctgattctg tcaatgttcc
     8041 ttttttgtcg ggataacttg ccatgaagaa tacccctttt aaatgtttgt ctgaatgatt
     8101 cttattgaca taacgacttg ccattacgcc acctaatgaa tggccaccga tcacataatc
     8161 ggaatttgga aaatcttctt tgaccttatc cgctttgttt ccgcccatca ctgctagatc
     8221 aagtggcatt ttgacaatag cgactgaaaa gccgtttttt gctaaatctt gcgcccaaat
     8281 actataactt tctggctcta cgagagcacc aggataaaaa ataagtaatg gagactttcc
     8341 atgattcttt ttctctcctt caaaaaataa gtaatcttct ttgttttttc cttcttttac
     8401 caacacttgc gcttctgacg atggttcgta cgtattttgc tgtatatagt aataaccggc
     8461 tccaccgagt aatcctaaaa tgatgactaa gccaattagt attcgtctaa atattttcac
     8521 tttctcccac cttttctatc tgagtgaccc ctttttctta atcaagtatg attatacctt
     8581 aaaaaaataa gagagaaatg caaactttcc agcatcaaat aaaaaaagac ggaatatcca
     8641 tttgaatatc cgcccttttt gtatgtttat ttttatttag cacataaacg atacacagct
     8701 tctgcataaa tttccattgc tttatacata ctacttaatt cccatcgttc attcgcctgg
     8761 tgcataaagt caggagtatc agggaacatc gcaccgaatg ccacacactg gttcatcgta
     8821 cgagcaaaag tagcgccgcc tgatacaaac ggttcagtca tgtctccagt aatatcgcga
     8881 tatgtcccca atagattttt cactaactca ctgtctagtg gcacgtataa tgaatcaagg
     8941 aagtcaaatt cttcataagt caacccatat ttttctgctg ttttagttaa tttagctact
     9001 aaatcatctt tcttgaatgt aacagggatt ctcatatcta caccgatttt tgtttgttct
     9061 ggtgtgattt ccaaacttgc aaagttcatc gttagctcgc cggattgttc atcttctgtt
     9121 ttgcccacga cagcttcacc agttgcattt tcttgaacta attttcctaa gaaattgatt
     9181 gggccaaaat caaatacttc tgaaagagca attgccagac gagaaatagc attcactcct
     9241 tgtgctgcat ctttcgcgtg gatactttta ccaagaacga cgatgccttc accttgttct
     9301 tcgaaatcga attcgtgttt tttcaatgct tcttttactt cagaaagctt ttctccagag
     9361 taaggagctg catcaggaac aacattcaac gctcctccag cttttacaga aaactcgttc
     9421 gttcctggtc ctgtcaagta agcttgcaat aagccttttt ctgcataaat taaaggaaat
     9481 tcagcatctg gcgcaaatcc ttgcgtaatt ccttcttctt tttcgttata tttttctaaa
     9541 caacgccata aattttcttc atctgtacca aagataaaac gaatacgtgt cttgaattcc
     9601 acgccagcat caatcaaagc tttgacggca tacattgccg caatagaagg acctttgtca
     9661 tcttgggatc cgcgaccaac taaccagccg tcttttatag ttggatcgaa tggttttgtt
     9721 tcccaattat tttcgtcccc tgctggaaca acgtccatat gacacaagat accgaataat
     9781 tcatctccag agccaatctc tgaataaccg taatagcctt ctggatcttt aaatgttcta
     9841 aatcctagct tttcactgat ttccaatact ttatccaaag cttcaatcac ttttttaccg
     9901 aagggatgtc cttgtcctga atctgcttca tctagtacgg aaggaattcg gatcaattcg
     9961 ctcaatgatt ctaatgcttg ttcatgatgt tgttctgtca caaatttttt catgatatta
    10021 tcctcctaat atgttaaaaa gggtcatgtc aaattcctcc gacacaacct cttcttctca
    10081 aataatgacg ttttaagcct gctccatcag caagcagaat gttcttaaca aatgatgaaa
    10141 gaaaaaacac tcactgaatg gagctattga cttgtccacg tgcttatcga tagccgttta
    10201 tcggtttctt cattcttatg atagaccttg tacttgtatt tgtctaggtt tagaacattg
    10261 ctgctacacc taggatcaca cagatggcta caaataatat cgcaattaat ttaccggtaa
    10321 atttccacca cactgaaaca tcgaaacgag caattgctac agcacccatt acgatagcag
    10381 atgttggtgt aattaagttt actaaaccag atgcagattg ataagcagtg attactaaat
    10441 ccttaccgac gccggcaaat tcacccatag gtcccatgat tcccattgtt gcagcagcta
    10501 agccggaagt tgaaggaatc aagaatgaca tagggatata gaaaataaat gtaataatga
    10561 tgaatacact tgaagataat gaacttaatc cttgttcgcc ccaatgtaag atggtgtctg
    10621 tgattaatcc attgttcata accacttgga taccacgagc aacagctact acgattgcta
    10681 caccgatcaa atcagaagca ccagctaaaa attcagagac aaattcagac tctttcatgc
    10741 cataagtgat tgctactacg atggacatta ctaagaataa catagtaatt tctgggaagt
    10801 accagtcacc taatggaagc atgcttttac ctagaaccgt acctagtacc ggaaggtttg
    10861 ttaaccagtt tgtcaatgat tcaaaaatcg tgaaattcgc attcaaggtt gtccaaggaa
    10921 tcaaactgat gatcatgata ccaaaagtaa taaagaacaa tacattaaca tgtttttgtt
    10981 ttttcgtgat tacttcttga cgaccgattg cttcaatatc gaaatgcttc atatcttctt
    11041 tacgttggtt gtatactaat gattttgttg gatctttttc gatctttgat gcataacgat
    11101 acacatatac aatagaaata gctaacaaaa taacgaacat caagaatcga agaccaatac
    11161 cttcacccat cgaaatccca acagcttgtg aagctacgcc tgttgcaaaa ggatttaccg
    11221 ttgaagctag acagcctact tgagacccta ctaatacgat tgctacagct actactgtat
    11281 caaagcctac agccatcatg actgggatca gtaaagcata gaatggaatc gtttcttctg
    11341 ccattccgta agtagagccg cctaatgcaa ataacagcat caatactgga ataagcattt
    11401 tttcttttcc tttgaatttc ttcacaactg aagcaatccc ggcatctagt gcccctgttt
    11461 tgttgataat acctaaaaat ccaccaacaa ccaaaatgaa gaaagagatt gggattgcag
    11521 caggtgtttc tttagttagc tcccccgctg gtacacctag catcccattg attggcgcca
    11581 tgaaaatatc ccacagccct tgaggatttt gttgtactcg ttcatatgat ccagcgacga
    11641 tgttgcctgc atcgtctaca ctatagtgcc cagctggtat aaaccaagta agtacagcga
    11701 ttactgcaat aataataaat aagaccgtat acgctgaagg catttgaaat tttttctttt
    11761 ttgtttgttc catcttcttc ttcctttctt ttccgttaga gacaacaatt ttggctctaa
    11821 tgttatttta tcaaaaagag tatcagaaca aaaataataa cacgcatttt gtaaaacttt
    11881 tgttcaaaaa gttcgttttt ccctattccc tcctcttttt tcaggttttt aaagctttct
    11941 tctttttcat tgtagcgttt cctttttttt ataaacaaac attttcgtct tttaaaagga
    12001 gaagaatttc acatttgcaa agaattgatt gatttttatg cataaatcaa aaaaatactc
    12061 aaaacaaaca aataaaacgt ttacattatt gcatgtcttg tatttttttc caaaaaaaaa
    12121 gaccaagaaa tttcttgatc tttttgatat ttatttgact gctgtatgac gttgttcgaa
    12181 ctgatgttgt tttcgactta accatggaag aagttgcgca tgcagcacta aaaagacagc
    12241 actcacgatg aatcctttga tcaagttgaa aggaacgatc ccaatcagta catagtttgc
    12301 taaagacatg cccagcatct gtgttgcgtt cagccctaaa aacattaagt aaagaggcgt
    12361 aataacgaaa taatttgcga tactcataaa aatagttaga gcgatggttc ctgtaatgac
    12421 acctaaaaat ttgtttcttc ctttatgctg tttctttaca ctaaagaaat aataaatcgg
    12481 gaatgtaaag atcgttgttg ataagaaact agcagtgtct cctaccaaat tatccggaga
    12541 aaaacctgta gtcagcagat gtaatactga acgcaagaac gctgttgcaa ctccggccgc
    12601 tggaccgaat aagaacatgc tgatcaatac cgagatatca ctaaaatcga tcttcaaaaa
    12661 actaaaagcc ggcatgatcg gaaaagcaaa atactgtaaa actaatgcga tcgcagcaaa
    12721 catcgctact gcaaccattt tctgtacacg ggtgttcttc atttgaaatc ctcctgttag
    12781 gactcctctt cagcgaactt tttgacgttt ctctccaaat gaaaaaactc tattttccag
    12841 ggaaaataga gttagagtta tttgatcaag cgtcgtcaaa taagctctat cttctttatc
    12901 cagactatac tgtcggtatc ggaatttcac cgattcagct atttttttgc aaaataggtc
    12961 gtggactata accaccggtc gggaatttca ccctgcccct gaagacgaac cttatataat
    13021 ttttgtttac atattcatta tacatatcta ttcaaaaaac gcaacatcta acacttataa
    13081 aaagtaagtg ttcattttga gtggttcagc aaactgctga cttctttttt gttcagttca
    13141 cggtaatccc caggacgtag gccttgtaaa gtcagatctc cgtatttttc acgttttagt
    13201 ttttgcacag gaagaccaac tgcttgaagc atatttttca cttgatgatt acgtccttcg
    13261 tggatcgtca attccacgat actgtggttt gtcttaggat ccactgataa aatttggaat
    13321 ttagctggag atgtttttcg tccttcgatc tttattcctt ttgtcaaagg tgcaagcatc
    13381 gattttgtag cgatcccttt tacctttgca acgtaaactt tatccacttc atgttttgga
    13441 tgtgtcaggc gctgagcaaa atctccatca tttgtcagta gcaagatccc cgaagtatcg
    13501 taatccagtc gacctacagg atagatacgt tcttttacca tcggcaaaag atccgtaaca
    13561 acttttcttc ctttgtcatc tgcaacagca gaaataactc ctttaggttt gtacaataaa
    13621 tagtaaccgt attcttcttg ataaattggg acttcatcca cttccacagt gtcatgtttc
    13681 ccaactttta tccctaattc ccttacaact tgtccgttta cttttacacg tcctgcaagg
    13741 atgaattctt ctgcttttcg acgagaagtg atgccagcgt gggcaattac tttctgcaat
    13801 ctctccatta caattattct cctttattca ttccatcaaa gaatagatct ggtgacacgt
    13861 cttcttcaag tgaaatttcc atttcttgtg tatcaggcag ttcttctaat gatttcaaac
    13921 caaaataatc taagaagtag ccagttgttc catataatat cggccgacct ggtccttcta
    13981 ctctaccttt ttcttcaatc agtttatggg cagctagacg ctgtaccgct cctgaaacct
    14041 gaactcctcg aatctgctcg atttcagctc ttgtgattgg ctgtttataa gcgatgattg
    14101 ctaaggtttc gacggcggct tgagataatg tgtttgatgt cccttgcgca tatcgcttaa
    14161 gtaagggagc cagctctttt ttagtcgtta atacataatg gctttccgtt tcaaaaatat
    14221 gcaaagctga gtgcgagttt tgagtgtaag attcttttaa ttctttcact aaacttgtga
    14281 cttctgcttc gctgagattt aacagtccgc ttaattcttc attactgatc ccttcctcgc
    14341 caacaataaa aagaagggct tcaagttcac ttaatttcgt catgttcggt atctcctact
    14401 gctggataaa gcataattga ttcatagttt tctttttgtt gcgcaatgat ctgccctttt
    14461 ttcatcaatt ctagcaaagc cataaaagta gtgacaactt cacttttaga aggtgactca
    14521 aaaaaagaat cgaaagtaca gccatgcgca tttcttttta tacgctcttc gataaatacc
    14581 attcgttctt cgatcgttgt gtcatcgggc gtgatcgttt tttccacttt ttgagaaaat
    14641 ttcttacgtt ctaagatttt ctgcatggca cggatcaaat cgattttttt gacttgtcct
    14701 cgcttcaacg gcttttcagc atccgctaac ccatcgatgt tcataggttc ttttgtgtaa
    14761 taaagacttc gttcctctgc tttttctgtc agtctttcag ctgcatattt atatttacgg
    14821 tattctaaaa gctgagtgac caattcttct cgaggatctt cttcccataa ttcttcatcg
    14881 tctgtgccga attcttggac aggaagcaat gtcttgcttt tgattgccat caatgtagca
    14941 gccatcacta agtattctcc tgcaacagct agctcgagtg ttttcattgt atggatataa
    15001 ttcatatatt gctccgtgat cgtagcgatc ggtatatcat atatatcgat ttccatttgc
    15061 tgaatcagat gaagcagcag atcgagagga ccctcgaata catctaattt aatattgatc
    15121 tccgtcaact cattcactct ttcttctttt tctttgctag ccattttcca atgtaaggct
    15181 ctgtgatttc agttgcacgg atggagacat ccgggtactt ttcctgtaat tcatctaaca
    15241 tttgaaagcc catattctca ccgcgataag acggattcaa agaaatgtca tggatgatca
    15301 gctgatcgtt cgttgcccag taaataccaa taatcccttg aatgttggtt gaaccttctg
    15361 gcgtaaagaa aaaaagttca taatcgtctt cagattcgta cgtatcaatc tccttcaaca
    15421 aagaagtttt acctgtcagt ttcttatgga aactcaatag tcccatagca attttacggt
    15481 tttctttccg gtaatgtgtt aacatcttct caccttccta tgactaagcc cttggaaaat
    15541 gctgcttata aacatccgtc attcttttct tcgtgatatg tgtataaatc tgagtcgtag
    15601 agatatccgc gtgacctaac aattcctgta ctgtccgcaa atcagctcca ttttctagca
    15661 gatgtgtggc aaagctatgc cgcagcgtat gtggtgttac ttctttgtaa atcccagcct
    15721 ctcgtactaa ctgcttcaga tttttccaga ttccttgccg agaaagacct ttcccatgat
    15781 tattgacaaa tacatgcatt tcagatgcgt cctttacaag taatggacgt gcttcgtcta
    15841 agtagcgttc cagccattgg atcgcataat cacctaaagg aatgatccgc tctttatctc
    15901 cttttccaac tgtctgtacc agtccaagag aaagatgaag atctcccaat ttcaatccaa
    15961 tcaattcact gacgcgcatc ccagtagcat acatgacttc caagattgca cgatctctta
    16021 tccctaacgt ttttgtcgta tcaggtgctt caatcagctt ttctacttct gtcagagtca
    16081 atgtacttgg taatttctgt gctttcttcg gcgtatcgat atgttgcatc ggatcatgat
    16141 cagtgatacg ctcttgccgt aaaaactgat ggaatcttct gagacttgag atcatccgag
    16201 taacagttgc tgtcgctttt ttttcttcat gcaacttctc cagaaaacgg atgactaaaa
    16261 agcgatcgac atcctgccat gaagcaattt tctgctcctg tagaaaatgc aagtactgtt
    16321 ccaaatcacg ttcatagctt tgtcgtgtat tggcggacaa tccccgttca atattcaagt
    16381 agtggagata atcgttgatt tgctctttca taataatccc cctcatgcct ctctatcata
    16441 tcagataccg cttcattctg atagtctttt tccataatct gagcaaagaa aaagccgtct
    16501 agacactcag acggactggt ttctttt
//
LOCUS       DAFEOM010000060        16483 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.063, whole genome shotgun sequence.
ACCESSION   DAFEOM010000060
VERSION     DAFEOM010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.063"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(<1..1835)
                     /locus_tag="LP524_002084"
     CDS             complement(<1..1835)
                     /locus_tag="LP524_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL2235111.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="LP524_002085"
     CDS             complement(1832..2536)
                     /locus_tag="LP524_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL2235112.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="LP524_002086"
     CDS             complement(2548..4857)
                     /locus_tag="LP524_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBL2235113.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="LP524_002087"
     CDS             complement(5052..5516)
                     /locus_tag="LP524_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235114.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="LP524_002088"
     CDS             complement(5516..6142)
                     /locus_tag="LP524_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL2235115.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="LP524_002089"
     CDS             complement(6149..6514)
                     /locus_tag="LP524_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBL2235116.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="LP524_002090"
     CDS             complement(6504..6842)
                     /locus_tag="LP524_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235117.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="LP524_002091"
     CDS             complement(6832..7158)
                     /locus_tag="LP524_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBL2235118.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7136..7417)
                     /locus_tag="LP524_002092"
     CDS             complement(7136..7417)
                     /locus_tag="LP524_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBL2235119.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            complement(7419..8783)
                     /locus_tag="LP524_002093"
     CDS             complement(7419..8783)
                     /locus_tag="LP524_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBL2235120.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="LP524_002094"
     CDS             complement(8796..9365)
                     /locus_tag="LP524_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HBL2235121.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="LP524_002095"
     CDS             complement(9337..10524)
                     /locus_tag="LP524_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBL2235122.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="LP524_002096"
     CDS             complement(10588..12315)
                     /locus_tag="LP524_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBL2235123.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="LP524_002097"
     CDS             complement(12312..12764)
                     /locus_tag="LP524_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HBL2235124.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="LP524_002098"
     CDS             complement(12876..13256)
                     /locus_tag="LP524_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBL2235125.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="LP524_002099"
     CDS             complement(13253..13639)
                     /locus_tag="LP524_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235126.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="LP524_002100"
     CDS             complement(13641..13919)
                     /locus_tag="LP524_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235127.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13967..14695
                     /locus_tag="LP524_002101"
     CDS             13967..14695
                     /locus_tag="LP524_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HBL2235128.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(14841..15317)
                     /locus_tag="LP524_002102"
     CDS             complement(14841..15317)
                     /locus_tag="LP524_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HBL2235129.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15277..15624)
                     /locus_tag="LP524_002103"
     CDS             complement(15277..15624)
                     /locus_tag="LP524_002103"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235130.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            complement(15611..15796)
                     /locus_tag="LP524_002104"
     CDS             complement(15611..15796)
                     /locus_tag="LP524_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235131.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(15797..16069)
                     /locus_tag="LP524_002105"
     CDS             complement(15797..16069)
                     /locus_tag="LP524_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HBL2235132.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16066..16269)
                     /locus_tag="LP524_002106"
     CDS             complement(16066..16269)
                     /locus_tag="LP524_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235133.1"
                     /translation="MVKVREQFKIVSDEDNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
BASE COUNT         4640 a         3471 c         2557 g         5815 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 ggtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 ttctgaaaat attttaatta aagattatta ccgtccgccg attaaaatag atttaccaga
    14281 aaatattgaa aaagtttctc ctacatttgt tgatatttat actcaagcca cacaagctaa
    14341 atctgagcaa ttggaacaaa ttgctggagt tggttataga aaagctctag aatttctaat
    14401 taaagattat gcaatatcta aaaattcaga ggatgaagat aaaataaaaa aaatgttatt
    14461 aggtaaaaca attgaaaatt atttgaatga ttttcctaaa ctccaaagat tagccaaagc
    14521 tgctacttgg attggtaatg atgaaacgca ctatgttcgt agacatacta ataaagatgt
    14581 tcatgatatg gatgcattta ttaaatcagc agcgcaattt atcgcggctg attatgatgc
    14641 tgatttagct gatcaattta taaatggaaa taactttatg ccaactacta attaaaagaa
    14701 taaaagagcc acagatacca attaataaaa cggttgggat atcttggctc tttcatattt
    14761 ttttgacact aatagaatat catgctgcta acaatgttta catagctaca atgaccgaac
    14821 atttagtgaa cagtgtaatt ttagaataaa gagagttgtt ctttgtactt ctcgctattg
    14881 taaaaatcaa tttcgttttc ttttttcaat cggtcagctt gtctgtgttc atactcatct
    14941 aaaaagttca atgtctttct aatttctgca tgtcgctgtc tgacatacga tgagctatac
    15001 ccaacagatt cagcaatatc ttctaagctc atttgatcaa catatttcat cttaacaatt
    15061 tgattatcga taccagaaaa gctatctatc agcaataaca tttcttcttt ttgctcgatc
    15121 aacaattcca attcattttc tatcttttgg atattctctt ctaaagaaga agttctcgaa
    15181 ttcttttcga tgcggacgtt tgctaaatca ccacagaccc atcgattcaa ttcaagctta
    15241 cttttattaa gattccactt taagtaaaga atctgctcat ctagttcttg gtaatctttt
    15301 aaccaatgaa atctcacaaa cgccacccct tatggtagaa tagtattgcg ctgctatctc
    15361 gaaaggggta gcttttttat tttttcttag ccgttccatt ctggattgcc ttttgttcca
    15421 aacgtcgttt tttcttctta atctttgatt ttgtttttcc catacaattc tcctttccga
    15481 aaacacattt tgttcgattt ttaatactaa ataaaagttg gccagaattt ttcgttcgtc
    15541 tttactcatc tttttttgaa taagattact agcataaaaa tagttagtac cacgaaaatg
    15601 aacaggtaaa tcaattacgt tcaccttctt catgccagtt atcagccact agaatctttg
    15661 cgttagtgcc gccaccgttt tcttttttgt atttttcaaa tgctttataa gcagctgttt
    15721 ttgatttata gatttttccg gaaccgactg ggaacatcca tgtgccctct gtgatagcta
    15781 agatgtatac tttcatttaa ttcacccctt taatccccaa cactacatat ccggacttct
    15841 gcatgtaatc agtaatataa gtgatctctg ctttgtatga gtcaccagtg taactaaatg
    15901 ttggtctcgt tagtccaggt aattcatgtt taatgtattt tttatattca cgtagtatta
    15961 actgatcacc aactttgaag tcacgatcat tcttgcggat ctcgaattgt ttgcgtccgc
    16021 tcgtcactgc ttcaaaatat tctggtagta tttttagttc gtggatcatt ctgtttcctc
    16081 cttcaaaatt cgctcatcat caaaaagtga atctccacaa aatccttctc ccaaatattt
    16141 atcgttcatg tataagtcaa ccaaatattt gtgatctgct tctcgatatt cattaatatt
    16201 aacgatcttt attttgtaaa cattatcttc gtcgctcact atcttgaatt gttctctgac
    16261 tttcaccacc gttcctcctc tttttgaaaa aattttagtg tttttgtaag accccctaaa
    16321 tcagcttttt tatttgatat aatacttgta tcgattaatc aaatcgaaat taaatttaaa
    16381 aaggtgattt atcatgaata tcaacaattt agtaaaactc gttgaacaac gttttaaaga
    16441 atcaggatta gaacaattaa aaaaagataa ttctccagaa tta
//
LOCUS       DAFEOM010000061        15532 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.064, whole genome shotgun sequence.
ACCESSION   DAFEOM010000061
VERSION     DAFEOM010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.064"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(<1..295)
                     /locus_tag="LP524_002107"
     CDS             complement(<1..295)
                     /locus_tag="LP524_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL2235134.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTND"
     gene            325..735
                     /locus_tag="LP524_002108"
     CDS             325..735
                     /locus_tag="LP524_002108"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235135.1"
                     /translation="MVTLFYRRDFLFSIYTKYFTLSSNIKTSTKLICKLFKLDKISNI
                     DDVKSKILTRKVSEPVKLTAQDKKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSG
                     EKVTFKINFITAISKLEYGKQKKRDNNDQYLPKK"
     gene            816..1436
                     /locus_tag="LP524_002109"
     CDS             816..1436
                     /locus_tag="LP524_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235136.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            2137..2421
                     /locus_tag="LP524_002110"
     CDS             2137..2421
                     /locus_tag="LP524_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235137.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            2424..3341
                     /locus_tag="LP524_002111"
     CDS             2424..3341
                     /locus_tag="LP524_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL2235138.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            3334..4116
                     /locus_tag="LP524_002112"
     CDS             3334..4116
                     /locus_tag="LP524_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2235139.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            4117..4797
                     /locus_tag="LP524_002113"
     CDS             4117..4797
                     /locus_tag="LP524_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2235140.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            4790..5686
                     /locus_tag="LP524_002114"
     CDS             4790..5686
                     /locus_tag="LP524_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL2235141.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            5881..7530
                     /locus_tag="LP524_002115"
     CDS             5881..7530
                     /locus_tag="LP524_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBL2235142.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            8124..9059
                     /locus_tag="LP524_002116"
     CDS             8124..9059
                     /locus_tag="LP524_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2235143.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            9052..9822
                     /locus_tag="LP524_002117"
     CDS             9052..9822
                     /locus_tag="LP524_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2235144.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            10238..11221
                     /locus_tag="LP524_002118"
     CDS             10238..11221
                     /locus_tag="LP524_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HBL2235145.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            11668..12702
                     /locus_tag="LP524_002119"
     CDS             11668..12702
                     /locus_tag="LP524_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HBL2235146.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTRAGS
                     NQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKILV
                     INGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELGI
                     DQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMFA
                     KTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHMLG
                     KVVVSGPFNG"
     gene            complement(12791..13114)
                     /locus_tag="LP524_002120"
     CDS             complement(12791..13114)
                     /locus_tag="LP524_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBL2235147.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(13101..13808)
                     /locus_tag="LP524_002121"
     CDS             complement(13101..13808)
                     /locus_tag="LP524_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBL2235148.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            13894..14235
                     /locus_tag="LP524_002122"
     CDS             13894..14235
                     /locus_tag="LP524_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HBL2235149.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            complement(14406..15254)
                     /locus_tag="LP524_002123"
     CDS             complement(14406..15254)
                     /locus_tag="LP524_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235150.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
BASE COUNT         4950 a         2663 c         3282 g         4637 t
ORIGIN      
        1 tatcattcgt ccgtctataa gcaggaacag tctgttgctt gaactcgccg ttgcggtcgc
       61 gcggaatctg gagatgaagt tccccgtact cggtcttgac cgtacggtca taggagccgt
      121 tacgagaatt ccccgtgtta aaaccaatgc gatcgtactt ttcgtaatcg aggaaagccg
      181 ttaactccgt tttgagaagc gtattgacag ctttttccaa atggacacgg aataattcat
      241 tcaaatcgcc tttgttcgct agagtcttta gaatttctgt agtaaaatcg ttcatatgga
      301 agtcctcttt tctgtgaatg tgttgtggta actctattct acagaaggga cttccttttt
      361 tctatttaca caaaatattt tacactctcg tcaaatataa aaactagtac gaaattaatt
      421 tgtaaattat ttaagcttga taaaataagc aatatcgatg atgtaaagtc caagatatta
      481 acaagaaaag taagcgaacc agtaaagcta acggcacaag ataaaaaaaa tggaattgtt
      541 ctttatggtg atgatgcaaa gtataactat aattgggata cctcctcaga agctgaggta
      601 ttaagtattc ctactaccga tacttcaggg gaaaaagtca cctttaaaat aaattttata
      661 acggcaattt ctaagttgga gtatgggaag caaaaaaaga gagataacaa tgatcaatat
      721 ctacctaaaa aataacgaat taatgatgca tatgttaaag ttgttttttt gaattaaaca
      781 actcaattta ttcagttata tgttcatcgc agcaagtgaa cattaatcga gttataaaat
      841 taataggagc taaaaatata aataaagttc gatcagaatt tatttttcca gaagattttt
      901 ttaactggtt tttttatcaa ttcagtgaat gcaagcgact cttaggtgag aattattact
      961 taaaaggaat cggcggattt atggggaatg tggctgatga acaaaatatt ataaaaggaa
     1021 tatctgaaca aacatcagag cttacagtaa ctaaagcatt tataagtaat ggtgaaagtt
     1081 tgaaaaatgt aactgcgcga attagaaaag aaaatgaagt agacattgtt tttggaattg
     1141 accatgaatg taattcagtg atatatgtaa cacaatcagc aaatttaatt ctttttgaga
     1201 atggttgcaa ggacgatttt atggtggtct atttgtacga tatactaatt ccaaaacttc
     1261 gttccttata tatgtcttct gaaagtattt ttgtgagtga acaaaaggga tctttctcga
     1321 aaaaaatagg tattgaagta attgaatcga ttatgtgtca taatcatatt acgctagaag
     1381 aattagaaat tttgataggg caaaaaaata ataacacgaa aatacaagtt tcttagaaat
     1441 cattttttat tgagatatct agataaatga tttattcttc aatcaaccaa ctcgccaagc
     1501 tgaggtaatg ttttgaggta atgataacat acaacttttt ataccctagt aaaactctat
     1561 aaacaaagac ttattaattt cgcatgaaat acattcctgt aaaatgcaat aaaatagagt
     1621 tttgtttaac caggtcttaa gaaagcccgt aaagcttcac agttctcaaa acgttaattg
     1681 cgattatcgt tcgagttcac gagttacgag aatcgtactg ggcgaagcaa tgctgagcgc
     1741 agttacctta atcctaactt ttctggcact ttccacaatt tggaaagtgc cttttttgtg
     1801 tttttagcta tgttaatagg taggattctt ggtctataat atcattcgat ctataaacaa
     1861 acgggaaaaa gataaagtat catcaaacaa tattatttat ccgcttctga ggttgtgaca
     1921 gaagtggtca gcttcaagaa ataagaagga atttacaaaa attgcttctc atgtttgata
     1981 aatattgtga cttgtgaccg aggagttggc aggagaacac cgtttattcg gaagaaggga
     2041 ttgtgaaaag tcttttgtca taacctctta tttggtagct aattgtaaat cccaaacggc
     2101 atgtattatt tttcataagt ccgtttttaa ggattaatgt tttcttttgc tttttatatg
     2161 gtctggcttc agcgagaagt tccaacgcat atacaaggac gtctgatgtc aacgataaca
     2221 acgattacca tggtggccag tccacttgga ttagcactgt ttggtgcgct ctttgattgg
     2281 cagacaactc aaccacgtct gcgagacagt ctgatttttc ttgaaattca attggaaaat
     2341 ttacactttc tttatacatt tctggctgct ttctttatac ccgtcaacta tacttcactt
     2401 atcaataaga aagaaggata agtatggatt ttggaaatga aacggtcttg aaagcaactg
     2461 gtataaccaa aaaatacggt gctgccaaag cattagataa agtatcgatc gaaatcaaac
     2521 gtggaatgat ctatggtttg attggggaga atggagcggg taagtctacc tttatgagaa
     2581 ctattatggg tttgatctcg attgatgaag gttcgatcga attatttgga acgacggatt
     2641 tgcaggcagc aagacgacgt atgggtcaat cgattgaaac ccctgctctt tatccagaat
     2701 taactgcaag agataaccta cgtatccaag cagcgaacgg cggtgtgtcg gatagagaaa
     2761 tcgaagacct gctgaaaatg atgcgattgg aaaatacagg gaaaaagaaa gcaaagaatt
     2821 tctctttagg tatgcgccag cgtttagcta ttgcaaatgc actgatcacg aatccagaat
     2881 ttttgatttt ggatgaaccg acgaatggga tggatccagc agggatggca gaaatgcgag
     2941 aaatcatcca acgtttggta aaagaacggg gcatcaccgt cttgctttct agccacttgc
     3001 tagatgaatt gtcacagatc gccactcact acggtatttt gcatgaagga cacttgatca
     3061 aagaattatc gaaagaagaa ttggcacaag aatctcgtca atttataaaa attgacactt
     3121 cggcgacgga acaagcagta acggttctcg actctcttgg ttatagggat tattttgtcc
     3181 aatctagccg tgttatccag ctttttgaag gaattgacca agtagcagcc atcaatcaag
     3241 cattggtaga ggcaaaagtt cctgtggatg gtatccacct agttgggcaa aaactggaag
     3301 attatttctt acaattaact ggaggcaatc cacatgttta atctattaac tgctgaaaaa
     3361 ataaaagtat ggaagagccg taaaatgtgg gtctcactag gactgatggt cgtacttccg
     3421 cttttttatt cttggaacga atggtatatg catacaaaat acggcaatga attaaaccaa
     3481 gcaacggata cagtcatcaa cggtgcaaca gggattttaa tggtggaaaa aatggctgca
     3541 tggattcttc tggctttcgc agcatttgcg tgcttttata ttggggaaga atttcaaaac
     3601 ggtacgattc gcaatacgtt atctttagga cggaatcgaa tgacttatta tgtgtcgaaa
     3661 cttctcgtta cgcttttgat cacaacactt ggcgtggttt tgattacggg gctagccatg
     3721 attgcttaca cacttgcttt cggatttggt gaagttgagg gaatcaaaaa ctatggaaat
     3781 tatgttctaa aagtatttcc tgtattgctt ctgctgatat tagcgacatt atctgttcca
     3841 gtggctttga cattcatcac tagaagtacc agtgtcagtt tgcttctttc ttttctttat
     3901 atcatgggaa cggccttcgt tcccggtgtt ttcgcaaaaa tcaaaggctt ggaatttcta
     3961 acggagtggt ttacggaaac gtggctcatg tatacggact ttgcgcaaca agcaacttat
     4021 tcgcaagcac cgaaaatgat tctggtcagt ctagtaacaa tcgtggtatc atcagctcta
     4081 ggaatgttta ttttccaaaa atctgacatt aagtaggtgt tcctgctgga aactattttg
     4141 attatcgaag acgatgaagc tgttcattct ttactggagg aggttcttga acagagatac
     4201 aaacttcttg atgcttattc aggtacggaa ggaaaattat tgttaagcac atatcctgtc
     4261 gatttgatcc ttctagatct catgcttcca gggctttcag gtgaagcatt gttagcagaa
     4321 atccgacaga catcgaatgt tccgatcatc gttctttcag caaaaagcga tcaaagagat
     4381 aaagtttcct tgttggcagc aggagcggat gactatgtga cgaaaccatt tgatatcgaa
     4441 gaactgttgt tacgaatcgg catccaactt cgtcatcaga cttctgtcca agtaaaagat
     4501 ctgtcgcaac gcatctatta caaggaaatc ttactggaca aagagtcgag ggatgtaaat
     4561 gtgaatggtg tggctgtcca tttgacagga agagaattcg atctgctgaa gcttttttta
     4621 gaacatccga aaaaagtatt ttcgcgagca aatctttatg aaacagtttg gcaagaacca
     4681 tattttgata gtgagaaaac agtgaatatg catatttcca atcttcgagg gaaactagca
     4741 aaggcacatg ctgtgtatat tcatacggtg tggggagtgg ggtttagatt tgattaagtg
     4801 gctgttgctt ctcgttgtta tctttatggg aagttttatt ttcttcctgc aaagggaaat
     4861 caagagatta actaagaaaa tcgccactct ttctgaacga caagaatatg gtggtcgtct
     4921 ttcagtcgaa tttcgagaaa agaatctaat ggacctagta gatgcactga atacatgggt
     4981 ggatgaaaat gaacaaaaag gacaagcatt tcaagaaatg gaagaaaaca tcagactatc
     5041 gatcgtcggc ctttctcatg atctcagaac tcctttgact gctattaacg gttatgtaca
     5101 attactggaa caggtagagg atacggagaa acgggagaag tatttaacga tcatcaaaca
     5161 atctgttgca agattactca gtatgacgga tcagttttat gatcttgctc gaatagagac
     5221 gaagcaaaaa gaaatggatc tccatacgct ttctttttct aaagaggtgg aagaagtatt
     5281 tttctctttt tatgaacaat tttctgagaa aaatatcgag gtagcgttcg ctgaacactc
     5341 gataaataca actgtattag cagatccgct catgttgctg cgagtgctgc aaaatatcat
     5401 acagaattta ttgcgttatg cagatggaca agcaaaggtg acattttcaa aagaggattc
     5461 attcattcga ttaacagtgg ccaatcctat caaaccagga gcgcaaacct ctgttgaaaa
     5521 agtcttccag cgtttttata ccgaaaatac cagccgcaca aatacggaat ctagcggaat
     5581 cgggctttac ctgtcaaaaa aattggtgga aggaatggga ggagagatga ccgctagatt
     5641 agacgggggc atcttctcca tctccgtcaa gttgcggagg gtttaattgg ctctaattta
     5701 cctgaagtga atgacaaaaa attgtatctc gttttaaaga catcttcttt tttggaaggt
     5761 gtttttattt tggtgtgatt gatacgaatt aggtaacgaa gacagttatt tttgtacccc
     5821 cttttcaaat agcgcaacag tgctatacta aaactaacaa atcaaggaac ggagagggat
     5881 atgggccaaa gagaatttat cgttttaatt attattgcag ttgttatttt attagttgtt
     5941 ttagacattt tttcacgttt gaaagtaaag gaaacagttc gtagtaattg gggaaaaatt
     6001 ccttatcaac cgagatttga taaagaagaa agtttgaagg atgcgtggct cacggagaaa
     6061 aaattccgtt catgggatag tgaaatcgac gatcttactt ggtatgactt ggatatgttc
     6121 gaagtatttg aagggatcaa cagtacctat tctagtgtag gttcagaagc gttgtatcag
     6181 cgtttacgta gctttgattt tggagaagat tatcagttag aaaaactaat tgctttttat
     6241 caggaaaatc ctcaactgag agaaagaatc caatatcaat tcgctcgttt aggaaaaaaa
     6301 gatcataatt ttgcaaaaca atatctagcg gatggaaaaa gtaaaaagat tggacgcgta
     6361 ggtttattta tttttcttgg cttgcttccg ctggtgggtg taggtttgct tcttttaggg
     6421 caagtactag gaatctatct agcaatcggt ggcttagtat tcaatacggt ctattatcta
     6481 gtgaagaaac aaacactaga aacagagctg aatagcatga gttatttggt ttcaacgatt
     6541 tcagccgcca atcagttagc aaaaatgacg acaccacttc aaaaagagct gaaacagcaa
     6601 actaaaccat taagagacat ccctaaatta ggtttttcat ttcgaatgaa gagcgggagt
     6661 gaagcagaat tgatttttga atatgtcaac atcatgttca tgctgccatt tatctcctat
     6721 catttcgttc tcactaaatt agaaaagcat tcaactgaag ccatcaagct gtgggaattg
     6781 ctgggagaat tggaagttgc agcagccgtt ttgaattatc gcacctatat gccgatcact
     6841 tgccgaccag agtttactga gggcggtgtg acagcgaaag atatctatca tccattgtta
     6901 aaagaagcgg tagttaatcc agtcaactgg cagaagaata cattagtcac gggttccaat
     6961 gcatcaggaa aatctacgta tgtcaaaagt atagccatca gctgtattct agcacagacg
     7021 atccaaacag cgattgcaga acagtttacc ctgcaacctg gacatgtgtt gacttccatg
     7081 gcaatcgaag atgatttatt tgaaggcgac agttattttg tttcagaaat caaatcgatc
     7141 aaacgtttgc tggaccaagt ggcttccaaa gaacgctgct actgcttcgt agatgagatt
     7201 ttaaaaggaa caaatacgat cgaacggatt gcttcttccg cttcagtcgt tcggtggctt
     7261 gcggagtatc catctttagc atttgtcgcc acacatgata tcgaattgac agagatttta
     7321 aagaattatt gtgagaatgt ccactttgaa gaacaagtaa cagaaggaaa aggaatcagt
     7381 tttgatttca aattaaaaga aggaccttca aggacccgaa atgcgattgc cttgctgaaa
     7441 gttttgaatt atcctaaatc catagttgaa caggcccaaa aagaaagttt gttgttcgat
     7501 gaacagcgtc agtggtatcc gtttgattaa ctttatcaaa cgaagcatga tgcgtgggtc
     7561 caaaaagacg attatcaaaa gaagtacatt gatgccaaaa aagtggcaga attcaatggg
     7621 aacgagtctg ctgtccagat gatcttggaa gatttagcaa aagacgatac aaaataaacc
     7681 acgctgtctt ccttctagca gcttttatcg aaaaaactta gcatcagcga cgagacgcac
     7741 gttccatggc aaagttctcc gtaaaacaaa cagcgaaaac agaaattgaa aataactatt
     7801 tccattttat ttatttcggt taaaataaaa gaaaaaagct ttcgataaaa ggacggagga
     7861 aagcttaaca tgaataaaaa agacaagaac ctcagttata aggatcttct atatgtcatc
     7921 gtagtagttg ctgtcggtac ttttatgatt ttaaaagttc cagagttgtt tagatagtta
     7981 ttctatcgaa gatgatcatg aactagaaaa cacatctcct ctttgttagc aggtgtgttt
     8041 ttgtgtggaa gccaagttgt tttttcatct gctgtattcc atgatacaat taagaatcat
     8101 gtctataagg agataggaaa taaatggcaa atgcgcttga aataaataat ttgaaaaaga
     8161 tctatggaag tggggtcgaa gcattgcggg gaatcgatct ttcggtgaaa gaaggagatt
     8221 tctatgcgct attagggcca aatggagctg ggaagtctac aacgatcggg attattactt
     8281 ctttagtcaa taaaacatct ggaaaagtga atgtcttcgg ttatgatctg gacaaggatc
     8341 tagttcgagc aaagcaacag attggattgg ttccacaaga gttcaatttt aacccttttg
     8401 aaacggtcca gcaaatcgtc gtgaatcaag caggctacta cggtgtctct cgcaaagaag
     8461 cgatgaaacg cagcgaaaaa tatttgaagc agtccaatct ttgggaaaag agaaatgtcc
     8521 gtgcacgtat gctttccggg ggaatgaagc gaagactgat gattgctagg gcattgatgc
     8581 atgaaccacg tttgctgatc ttggatgaac caacagcagg agttgacatc gagttgagac
     8641 gcgagatgtg ggagtttcta cgagaattga acgaaaatgg tacaacgatc attttgacga
     8701 cgcattatct agaagaagct gaaatgcttt gtcgcaacat cggaatcatc caatcgggtg
     8761 aactgatcga gaatacaagt atgaaagagc ttctgtcaaa actgcaattt gaaacattta
     8821 tctttgattt agcgccatat gataccaaac ctgtgattga aggatatgct tgtacatttg
     8881 aagatgagca gacaatcgct gtcgaagttg aacgaaatca aggggtgaac ggaatcttcg
     8941 atcagcttac aagacaagga atcaaagttc tttctatgag gaataagtcg aatcgtttag
     9001 aagaactgtt cttgaaaatc acagaagaaa ataatcatac ggaggagaaa aatgtttaat
     9061 ctttatttta ctgcattaaa aagtttagct atcaaggaaa cgaaccgcta tttgcgtata
     9121 tgggtccaaa cactggttcc gccagtgatc acgacatcct tgtattttgt tattttcgga
     9181 aaaatgatcg gtgggcggat cggtgacatg ggtggttttt catatatgga attcatcgtt
     9241 cctggtttga ttatgatgtc tgctatcact agttcgtatg ccaatgtttc ttcgtccttc
     9301 ttctctcaaa aatttcagaa aaatatcgaa gaaatcttgg tagcacctgt tcctacgcat
     9361 gtgattattt ggggctttgt gattggtggt gtgggtcgca gtattctcgt cggctctctc
     9421 gtaacgatca tttccttgtt ctttgtacca cttcatgtgt attcatggtt catggtcatc
     9481 attacattat tgatgacggc aatcttgttt tcattagcag gactgctcaa cgggattttt
     9541 gctcagtctt atgatgatgt atcgatcgtg cctacatttg tcttgcagcc gttaacttat
     9601 ctaggtggcg ttttctatgc catctctatg ttaccgccct tttggcaagc tgtttcaaaa
     9661 gtcaatccga tcgtttatat gatctccggt ttccgttacg gattccttgg aacgatcgat
     9721 gtgccagtag caacatcgat gatcgtactt gtgttattca tcgttgtttt gtacgccgtt
     9781 acttggtatt tgatcaataa aggaagagga ttgagaagtt agaaaagagg ttgtgaaatc
     9841 agcgttttga cctgagtatt agagaaacaa acgggaaaat agttcctcat attttgttgt
     9901 tttttgagcg aataccgagg gtcagctgat tgaacaccgt ttagtggaga ggttgtgaaa
     9961 aagctgttct gcatcaagta ataagaacag ccaaacaaaa atagctcctc ctatttttcg
    10021 tttggttgga cttattaccg cagatgcaag cttttgaaca ccatttattc cggaaaatgg
    10081 actgtgataa gatttttgtc acagtctttt ttttgctcaa tcagaaacat cttttaagct
    10141 gttcactttg cttctttttt tgtttcctag tataatgaca atgtttttga gaaagattgc
    10201 gacttcactg cttgatagaa tggagagtaa caaataaatg actactaatt tttggaaaga
    10261 gctgccaaag cctttttttg ttttagcacc aatggaagat gtaacagatg ttgtatttcg
    10321 ccatgtcgta aagcatgcag cagcaccgga tgtcttcttt actgagttta caaactcaga
    10381 cagtttctgt catccagatg gaaaagacag tgtacgagga agattaacat ttacagaaga
    10441 tgaacagccg atggttgccc atatttgggg agacaagcca gaatttttta gagaaatgag
    10501 cattgctatg gcagaaatgg gcttccaagg aatcgatatc aacatgggct gcccagtccc
    10561 taatgtagca gaacgtggaa aaggcagtgg cttgatcctt cgtccagaag tagcggcaca
    10621 attgattgaa gcagctaaag ctggcggttt gcctgtcagc gtcaagacgc gtatcggcta
    10681 tacggaaatg tctgagatgg aagaatggat cagtcaccta cttcatcaag atatagccaa
    10741 tctttctgtc catttgcgta cgcgtaaaga gatgagtaaa gtagatgcac attgggaagt
    10801 cattcctaaa atcattgctt tacgtgacca aatcgctcca caaacactaa tcacgatcaa
    10861 tggagatatc cccgatcgcc agacaggaga agaattggcc gagaaatatg gcgttgatgg
    10921 catcatgatt ggccgaggga ttttcaagaa tccttatgca tttgaaaaag acccgaggga
    10981 acatacagaa aaagaactga tcggactact tcgtctgcaa ctagatctgc aagatcatta
    11041 ttcagaaatc atcccgcgtt ccatcacagg actacatcgt ttctttaaaa tctatatcaa
    11101 aggatttcca ggagcaaatg acttgcgagt tcagttgatg aatacgaaat ctactgatga
    11161 agtacgagag atcttagaga cctttgaaaa agaacatcca acgcttttca gtgaacaatg
    11221 atttcttgct tgaacaccgt gaattagagg tcgtgaaaaa gctgtcctgt atcaagtaat
    11281 aagaacagcc aaacaaaaat agctcttcat attttgtcgt tttttgagct aataccgagg
    11341 gtcagttgat tgaacaccgt ttatatcaga ggttgtgagc ctgccgttta actctgagtc
    11401 acaaggagca atttcaaaaa acagcttctc atgtttgatg aatattttga cttgtgaccg
    11461 aagagttggt aggagaacac cgtttataag aggttgtgac aaaagtcttg tcgcaacctc
    11521 ttttttaacc aaaaaagtgc aaatagttta ctttaagtaa gagaataatt atgatgaata
    11581 aaaaaggtgg aacaaatagc atactatata tattgaaatg aacactgtat ggatgctcca
    11641 acttgatgaa gctaaggagg aattcgtatg tcagaaaagg aattaatgaa ggtagtcggt
    11701 ttttatgaag ggttgccgat cgatgatcca aaaagcttta ttgatgaaaa agaacagata
    11761 cctgtaccca gttcacggga tcttctggta aaagtcaacg cagtatctgt taatcctgtc
    11821 gatacgaaac ttcgccaaga taacgggata agaaatgctt tacgtatctt aggttttgat
    11881 ggagtcggga aagttgttgc tgtaggtgac gaagtgcaaa aattttctgt cggcgatcgc
    11941 gttttttacg caggtacaac aagagcagga agcaatcaag aataccaact agttgacgaa
    12001 cgtattgtcg ctcttgcacc aaaaaatctt tcagatgaag aagctgcagc cttgccgttg
    12061 acctcattga ccgcttatga attgttattt gaaaaatttg gtctaactcc agaagaaaat
    12121 gccaatcgcg ggaaaaaaat cttagtgatc aacggatccg ggggcgtcgg ttcgattttg
    12181 aatcaactag cgcattgggc tggcctagaa gtctacgcga ctgcaagtcc gaagaatttt
    12241 gaatggctga aaaaaacagg tgtagactat ccaatcgatt atcatcacga cttaagtaaa
    12301 agttttcgtg aattaggaat cgatcaagta gagtatgtcg ctgtattatt cgatattacc
    12361 cgctactttg atcaaatcaa gcaccttgtc cgaccatttg gacatgtggg aacgatcgta
    12421 gggatcaaag agccgttaga tataggtgtt tggaaaaacc aatctatcag cttcgactgg
    12481 gagtatatgt tcgctaaaac agactataac tatgaaatcg aaacgcaagg agaagcttta
    12541 gcgcacattg ctgctttagc aaatgaaggg attatccgca gtacattgag caaggtctat
    12601 tcagatggga tgaatgcgaa gaacttgaaa caagcaacca aagacgtaga aacaggacat
    12661 atgttaggaa aagtcgttgt tagtggtccg ttcaacggat aagcaagaat acgccgattt
    12721 ttttcttatt tgactttgat aaaaacaaga gcgcatgaca gtaatttgcc ataagctctt
    12781 gtttttttat ttataataag cgaatcaacg aaagggaaag aattcctgcc aagacgatca
    12841 ccaataagct ttttgaaaga atagcagcta aaagtgtagg aactgaagcc aaagcatttt
    12901 cgagattgat ctgcggcaga tgtccgatat tttgggtaaa tagactgctg aaccataaag
    12961 cagacataat gacgatcggg acaaaactta aatactcaag caacggttgc ggtaaatcga
    13021 attttttcag taagacaaat ggtaggaccc tagaaagcca agttgctaaa gtacaaccta
    13081 aaatagtcag caatgtaaac tcaaaagaag gcatgtttga tccacacccc caatccgcac
    13141 ccaatcagtg ttacaactac aatcactaag ttgcttggga taaaaatcag tccgacatat
    13201 accagaatca aagtgagacc aatcaggatc aactgtaatc gtttagaggt gtttctatca
    13261 gaaatgactt gaagataaag taacccgata aacatagcga cgatggcgaa atccaaacca
    13321 aactttttcg gatcagtaat aaaattccct aaaagtgcgc caacaagaga agaacccacc
    13381 cacgtcaaat aagaaatcca attagctgta ttcatccaac gaaagtttaa cttttgtcca
    13441 gtatagttca atttattcat tcccagtgcg aaactttcat ctgtcagcaa tgtgccaatc
    13501 agcagatttt gaagcagccg atttttcttg aaataaggag caatcgtcat actcatcaga
    13561 atcattcggg aattgactag aaaggtcgag aaaacgatgg aaagcaatgg gctgtgacta
    13621 gcaagcatgc tgactgtgat aaattgggca gaaccagcat agaccagcag ggacatcaac
    13681 gtgacgacta gtggatgaaa tccggcagct ttcccaacga taccaaatgc aagtccaata
    13741 ccgatataac cgaaaacggt aggtaacgtg tctttgatcg cagtttttat atctaacttt
    13801 tcatccaata acaaaactcc tttagcacga cataaatttt cataattgtc gttaaacttg
    13861 tgaatattct aaatttttaa tgagcatttg tcaatggtgg gattgaaacg taaagaacta
    13921 gcgggtcgca gatttacacg tattgtttcg gtgggaggag gcgcgaaaaa tcttgcatgg
    13981 ctacaaatcc aagctgatat tttcgatgca acggtctatt gtttaagtgt ggaggaagga
    14041 cctgcattag gcgcaacaat gattgctgca ctagggttag ggtggttcga cacacctgaa
    14101 agttgttgtc agaattttat ccaatataca gatgcagtgg aacctctctt acaaaatgta
    14161 gaaaagtatc ataaaatcta tcaacaatgc cggaaaatct atccagcaac gaaggaaatt
    14221 tgtcatgact tataaaacaa aacgcgtacc taatagtcaa atgactaata ttgggtacgc
    14281 gttaagtctt ctttacttca tcctttgcat gattcgtgtt tttaaagcaa ggtctgctgt
    14341 atttcttgtg gatttaggat ggttgttatc gtttacttgc tatttttcat tgaaatcgtc
    14401 tttctttacc gtttctttag ttttttatct atttttttct tttggttaga aaattctttt
    14461 tcccgaaagt cttctttcag gatttttata gagtagcgtt ttgaataaga ggcgtcagta
    14521 tgatcgactt ggcagacgaa ccaagagaac atttgccttt tttctgcatg aggtgcttct
    14581 gctaataatt ctttccataa ataataacag tcctctatca gaagaagggt tcgttcatcc
    14641 tcaggctccc aatagaatgt ttcgagagta gcaatcactt cattgatcaa ttcgaatgcg
    14701 ggaagatagg cattttgctg aataagcgat tcaactgctt cttggatgaa ctttcgcatc
    14761 tccatgatga acttcgctgt tttcctgttt ttgtttttct tgataatatg atcgtattta
    14821 tggatgatag ttgttaattt atctgaataa tttattgcat gaaatggcat cttaactttg
    14881 aagcgttcga taaagtgcgg atgttctata agaatctctg ttaaaagctc ccgtacaaga
    14941 gaggaactag cggaggaaac aagggacgag agttcggaaa gtgagtagtt ggattttttt
    15001 attggacgtt cttcgtaagc gaataagaca gcagccatgt gtttgcattt ttttccttct
    15061 atggcatata gacaatcaca agtcatgttg gtcttttcat cccaagtgat cactacctta
    15121 taatcttccg tgccagaaac tgttgcggac aagtacttgc ttgtatagtt caaatgacgg
    15181 acaaaaccgt gcagatgata gctgtaaccg cgttccaaaa cgttttcagg aaaatactca
    15241 agccaattat tcatctagtt ctcctttcat gaaacactta tttatattta gtataaagaa
    15301 acgtttaaga aaaaagtagc tgattttaca tatttgataa tcacttttgc tcgaattgtc
    15361 aaaaattcat tctccttttc ttttcttaga gaatgtgaga aaaagtaaaa gaatagcgtg
    15421 ttttaaagat ggaacttttt ttgtttttta tttatctgac aaaaatgaag tgattttgtt
    15481 agaaataaat aattgttcca attgataaaa gaggtatgat gtatcgcaaa ga
//
LOCUS       DAFEOM010000062        15463 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.065, whole genome shotgun sequence.
ACCESSION   DAFEOM010000062
VERSION     DAFEOM010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.065"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(<1..1118)
                     /locus_tag="LP524_002124"
     CDS             complement(<1..1118)
                     /locus_tag="LP524_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBL2235151.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDG"
     gene            complement(1201..1536)
                     /locus_tag="LP524_002125"
     CDS             complement(1201..1536)
                     /locus_tag="LP524_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HBL2235152.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(1546..1920)
                     /locus_tag="LP524_002126"
     CDS             complement(1546..1920)
                     /locus_tag="LP524_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL2235153.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(1933..2247)
                     /locus_tag="LP524_002127"
     CDS             complement(1933..2247)
                     /locus_tag="LP524_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL2235154.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(2360..5587)
                     /gene="fss3"
                     /locus_tag="LP524_002128"
     CDS             complement(2360..5587)
                     /gene="fss3"
                     /locus_tag="LP524_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HBL2235155.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            complement(6001..7167)
                     /locus_tag="LP524_002129"
     CDS             complement(6001..7167)
                     /locus_tag="LP524_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL2235156.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            7192..7518
                     /locus_tag="LP524_002130"
     CDS             7192..7518
                     /locus_tag="LP524_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235157.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            7671..8723
                     /locus_tag="LP524_002131"
     CDS             7671..8723
                     /locus_tag="LP524_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBL2235158.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKSKMQMFPNFYYLLKPVLCKDDVYKINKITHQENRQKNLIVQNLNYGT
                     NQYSVDSSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLLESLS
                     TKNNKLDEELIETFIEENLLKQIWGESIVNCLKLASNSDYRQFDNWYKKFKYAIRSAE
                     KEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNK
                     ALELIELQISEGIDKQ"
     gene            8799..9017
                     /locus_tag="LP524_002132"
     CDS             8799..9017
                     /locus_tag="LP524_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235159.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            9004..11577
                     /locus_tag="LP524_002133"
     CDS             9004..11577
                     /locus_tag="LP524_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235160.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKF
                     DSNHETVIIDSEIRENDKGSKSRRRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSC
                     N"
     gene            11568..11765
                     /locus_tag="LP524_002134"
     CDS             11568..11765
                     /locus_tag="LP524_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235161.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            11769..12380
                     /gene="mobC"
                     /locus_tag="LP524_002135"
     CDS             11769..12380
                     /gene="mobC"
                     /locus_tag="LP524_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBL2235162.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            12365..13756
                     /locus_tag="LP524_002136"
     CDS             12365..13756
                     /locus_tag="LP524_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBL2235163.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            13972..14286
                     /locus_tag="LP524_002137"
     CDS             13972..14286
                     /locus_tag="LP524_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235164.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            14279..14773
                     /locus_tag="LP524_002138"
     CDS             14279..14773
                     /locus_tag="LP524_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235165.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            14841..15155
                     /locus_tag="LP524_002139"
     CDS             14841..15155
                     /locus_tag="LP524_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235166.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            15254..>15463
                     /locus_tag="LP524_002140"
     CDS             15254..>15463
                     /locus_tag="LP524_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBL2235167.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDL"
BASE COUNT         5203 a         2600 c         2778 g         4882 t
ORIGIN      
        1 attccgtctc ctaaatattt cgcatccggc ctttggcaag ccaaaatcag aaagaaacca
       61 gattgacgac ccaacatcac gatttgtttg agcttattca aaatcaccgc actttctttc
      121 gtcgttaaca tctccatata agccacgtat tcatcaaaga ttaaaaagtt gggtggaagt
      181 ccaagatagg cataattctc tcctgttttg tagttagaca tttctttcat tgccttgcta
      241 cgagccatca tacgttcata gaaatcttcc acacaagcag aaatttcttc cttttgtgaa
      301 taaacatgag gcatcaccgt acctaaatca gctaaatctg catttttggg atcgaggata
      361 aacaattctg catctgactt caatagtgct tcgataatgg tgaggaggaa ataggtcttc
      421 cctccacccg tgccaccagc gattaacata tggggtaaag aatcataagc ccatacttga
      481 tttttcatca atcgcagagt cccattttct gccactactt cgtctatccc aattcgattg
      541 gcgatcatat catacagtaa agtatactcc acataggagt ctttgagttc cttttctact
      601 aactcacaat acagtccgct ttctaacttc ttttccaatt ttaaaagctg atcttggtat
      661 ttcccaagtg aaatttgaac gcgaatagac agtaatccat ctttcattcg ataatagatt
      721 ttggggaagt aactgatagt ctctctggtt cggctactat tcaaatcttt gaaaaagcct
      781 tcactttgag tttgtttcac ttcataccaa tggttctcta gaatcatccg agctagcttt
      841 tgtcgatgcc acaagcttcg ccaatgatca agaaaaaagt gaatataaag caatatcata
      901 gctcctgaaa aagcgagact tccaagcaga catactaaat cttttataga ccaaacgatt
      961 tcaatagtag aaagtgatag cttagaaata tcaaattgtc taaactcttg aaaataaaag
     1021 acgctcatcc aaccgatgaa cagagcgagt agccagccaa gaacaaagtg ataaaccaag
     1081 tgctgatctc ctgcgcgtat gcgatgtcct ttgtacattt tgatggtttc cacctccaat
     1141 cgaaaaagcc caataggatt tcattaggca atttcaaaaa gttatgctag tcatttactg
     1201 ctacgaggag gggagttgtt tcgtttcaac caattgattt aaaaagcctt caatagcatc
     1261 cggcatctcc acaccaattt cttccaaaat ggattctgcc tgccgacata agttttcatt
     1321 tacttcgaca actataaaga aagactcttc ttctcctaac cagtctagta catcagagtc
     1381 ttcttcactg tcgctatttt ctccaagtaa accttgatgt ataatcagaa acattttttc
     1441 atcagaggtt aatgctttaa cgagttgttc ctcagtcagc tcgccttttc catattgatc
     1501 gagacaatct aaaatatgtt caacattaat ttccataatc ttcacctatt tctttctttc
     1561 attttggttc gtaggatttc cttgattcgg ctgccgcttc aagactaaat catccgcctt
     1621 aatgaaccaa ttaacggtag ttccttctcg aaaaacataa ttggcaacgg tatccacgat
     1681 tggattgacc aactctacct ctgcatcata agcaaactcg cgtaagggta cattagctgg
     1741 gatactaact tgaatcattc gcccttgtgc agaggatttt aaattatagg ttcgttcttt
     1801 tacctcatta ccaattgttc catcctcatt ttgcttccgt acttctcggc gtaatgcgga
     1861 aaaacgcaac ttcccaaatg ttaaacctga atctacgaca ataccttctg ttaatctcat
     1921 cttcttttcc tcctatgctt tcactaaatc atcaacatac aacacgtaat cagtaaaccc
     1981 acgattctca atctcatagc ctttggcaac caaatgggga ttcacggctt ttagcggttg
     2041 gtcttgatca aaatcctttt caccggcttc cgcagctact accacctcaa tatcatcggc
     2101 ccgttgctta ctggaataca aatgataact gcgaccaatc accgtagtat tccgtccata
     2161 tccttctgtt ataatttctc cttcaccggc ataacttaac tttccaaagg tttcagccat
     2221 atctggcacg acaaatttca attccatctt cttttcctct tttcattgtt agttttggat
     2281 acaaaaatag cgcgactact tttttaggag gcggtcgcgc acgttggttc ttaggattta
     2341 cttgtatcat ctcatcacct tagttccggc gattccagaa ataatagccc gccgtcgcaa
     2401 aaatcaaagt aaaaccaatg aacagtaaaa cattggaatt tttctccccg gtttgaggga
     2461 aggtcttcac tgtgggacta gattcttgag agccacttgg aacagttggt gtttccggtt
     2521 gtttcggcgt acttagtatg gttggaactt cttttggtgt taaggtttga gatttttctt
     2581 tcaaatcttc attgtgtttt ccattgatat tcccatcttt atcgtaattg atttccgcaa
     2641 aagtaaactt ggttccttct ggatattttc cggtatctac ttttttcgca agcacagttt
     2701 tggtgaattc tttatcattt acttcataat caatcttgcc agatttccaa atttctttgt
     2761 ttgtaccatc cggtaataaa gcatacagaa ttgtttcgaa agcttctttt gagccatcca
     2821 gtacatcatg agtaatcgac acatcatcaa acatatccac cacatcacca tgagtaaagg
     2881 tctgagaacc gttttctagg tgggcctttg tttggataga aacatggtgt tccactgtac
     2941 aactgacagt ttgggcttgg ttattcaagc taacatcctt cgctactggc ttatcgcctg
     3001 cttcaaaagc ttccttattt tcatacacat agttgaataa gacaatagtt ttatttaaaa
     3061 cttgttcggc ggttaattta tgctgaattt tccaagttcc tgttttctct ttgttcgcca
     3121 acgttgcgct tgtttctgca atcaccactg gtttggcttt ttcatctttt tcttgggcag
     3181 cttttgtcgc atccacatca atggcttgcg ctaccacata ccagtcttct ttgatttcat
     3241 gtagattgta actcaattta tcaactagct cggtagaatc cagagaagtt aattctttgt
     3301 ttccgtcttt ccaagtcgca agagaagtta aactatcttc ttcctcaggc aaatcataga
     3361 gcattaaacg gttcaaacta acagaaaatg ctttgttcgt cagcttctcg taaggaacgg
     3421 tcaccgtttt aattggttgt ttttggtctt gttcggtaat ggtaaagaca tactcactct
     3481 tcacaaagtc ttctttgttt tccttaaaag tagaacggat ttctaatggc gtaatctttt
     3541 gaaaaccttc tggggcttct acttcttcaa gtaaataatc cccataagga agattttcaa
     3601 acttgccata accatcaaac cctaattgct cgttataagc tgtggtcgct tcatcttcgg
     3661 cacctgtgat ttcattggtc ccttccaatg gcgacacttt aaaggtcaga tcattgaagc
     3721 cagtttcggt agtgcccgca gctgatccag caaatttaaa gaaatcaaag ccaaagcgaa
     3781 tcacttgttc ttttgccgta acatttcggg taatcacggc atttttttcg ctatcatcca
     3841 cctttttgat ggatacagga tactttgttt catcaaggct atatccttcg ggcgcttttg
     3901 tttcttgcca ataatactcg ttgatagcta aatgtttaac ggcaacttgg ttcttttcgt
     3961 ctaaagtcaa agtgaccgtt tcatcagaac ctttcgttcc cttcgctaac tctggtttaa
     4021 aagcctcact ccatttaaca gcttttccat ccttagcagt aaacaaggtg tattcagcac
     4081 cctcaaagac tgccttacct tgtgctttat ccccagtatc tttgtcttct ttcgtcaaag
     4141 tggtttcgcc agtgacttct tgattttgcc cttttacgtt actggtaaca agggctacgg
     4201 tttgattggc atacttcaat tcgactttta caggtttgaa cgtattcacg aacccatgac
     4261 tagctttagt ttcggtcaca taataggttc ccaattccaa cgcattagca atctcttttg
     4321 gtgtttccgc atgaccgttt tcatccgtgg tcatttcttg aacgatttca ccggttggac
     4381 tgtctttgcg aatggcaaac gtattaccag ctaaggaata attgtcattc caaagatcac
     4441 tccctgtttc aactccgctt ttatccagaa tgatttgccc tttttcccgt gcgttttttg
     4501 aagtcacgta aatggtttcg cctgctttaa tggtggtagt catgggggtg gtatcaatcg
     4561 tataaggcgc cggtactgat ttttcggtaa tggttacttt tgtaccatga ggaatctcat
     4621 ccaatgtagc aatgccttct ttgtcagtag tcacgtcttt tgcaggtaaa gtttttccaa
     4681 agtctaaatg gaaaaccgtt cctggtacaa catcacccga ttctttatcg acttttttga
     4741 tctttaaaga accctccgtt tccacatcaa ttttgattgt gtaggtatta ggtttatcaa
     4801 tcgccccggc cattaaagtc tgttggccag cctttttata ggcgacaggt gttccggtac
     4861 cagcagattt cttaagcgtc agcacaccac tttcttttga attggcattt ggggtgatta
     4921 tcaactgatt accattcaca cgataatcaa tgtttgcggc attttccaca acctcatcga
     4981 actctgacaa attcagatta ttcgtatccg tcacagtagt cgattgccct aatactgttt
     5041 tagccgtact attatggaag gttggtttct tctgataatc gtcaatggct tgatttattt
     5101 ttgcctcaat ggctgcgata tttacggcac taccatctga tcgttttatt gagtgaaggg
     5161 tatacccatt aacttcttgc cagatcatct tctgtgccac aatatgagta tctgcatctg
     5221 tacctacttt tttccataaa acggaaacta atttggcttt atcggacata tctggtaacg
     5281 ggtttttctc atagccagga gtgaactcat tgtaaatcgg gactcctggt tcaatacaga
     5341 aaatatcttg tttaaagccg ttgtacactg cataatgcgg aacagtgact tctgacattg
     5401 gacgaccatc actaaaagtt ccttcaacag catattgatg tgctaaatct agttctacgg
     5461 ttaccgtttg tggatgggta atttcatcag caaaagtaat cgtaggagca attgctccaa
     5521 caattgcttg aacagccaaa ctcaaaatag ttaatacaca taatttcttt ttttgttttt
     5581 tatacatgaa atgttctcct ttcaaattaa gcacaaaaaa ataagatgct cgcttgaaca
     5641 tctttgttta taaagtcttt ttaaaatttt tattgactga atagaatgga tttgagaatg
     5701 aatgagccta aaaatttaat ctaaaatacg aaatatactg cctttaaatt agaacacatt
     5761 ttagaacaca aacatttcaa atttaatcaa atataataaa atatatttac gggaaaacct
     5821 ttataaatca aggtttttca aacttattca atgatacgaa gaaaccaggt cttaagaaag
     5881 cacgtaaagc ttcacagttt tcaaaacgtt aatcgttgtt ataccaacga tttcagacac
     5941 tttctcattg cgagaaggtg tctttttttc gttctggtaa tgagaaaggt aatgacaata
     6001 ttataaacca gtgtaagcaa tgaatttttc taacgcattt cgttgagctt tttcagtaac
     6061 atgagaataa gtgttaaggg tagtttcagc atttttatga cccaatcgat cttgtgtttc
     6121 tttgatactt gctccacctt ctagtagaag agtagcgtga gtgtgccgga atccatgaat
     6181 agtaacgtgc ttaaatttat atttcttctc taaacgtttc aatatattat ttgaatagtc
     6241 cgcatgcaaa ggttgattaa tgtcaccgtc tgcatttaca taggtaaaga taagttgatt
     6301 ttttgtattt gtatttatgc caaactgcaa aagctctcga gcttgttctt ttttccagat
     6361 ttttaacatt tcaatagttt gtgcatcaat tggtaatatt cgttcggaag atatattttt
     6421 tgtatttttt tgaatcctga cagtttttct tcttgcattc ttctcttcat ttcgaatagt
     6481 tgcatagctc tttctaacat acattagctg ttcttcaaaa taaatgtcag accaatgaag
     6541 agcagctagc tctcctttac gtataccagt agtaatcaaa agctggaaga ttaaaatatg
     6601 ttttaaaggc tcttctttat tgagaatatc ccaaaaagct tttagttctt ctaatgaaaa
     6661 gtaattttca ttttctttct tttcccgctt tggtagattt gtatttgcca taggatttcg
     6721 gtgtaatagc tctgcaaaaa tggcatactc aaatatttga gagctgtaag acttaagctg
     6781 tttgatattc ttgaatttta aagcccattc attgactgca ttctgacagt gaacaggagt
     6841 aatcttagaa atggacttat tcccgaattt tggtagtata tgcaagcgga aaaggtcttt
     6901 tgtacgttga tatgtcgttt cttgtacacg attcctataa gaaggcaacc attcttcata
     6961 cactgttata aacaagtcat tagaaggctt atgatagccg gtatcttcaa tctcgacctt
     7021 ttttcttgct tcgtgtaact tggcttcttt ttcagtttta aacccttttt ttacatagta
     7081 ttcttgcttt ccattttcat ctttacgaac atacaagcgg acactatatc gtgcaccgct
     7141 ttttgttgtg tacttttgta ttgacataac taatctctcc aatctgataa cttggcgagt
     7201 tggctgattg aagaaataga aaatgagagg agaaaaataa tggatgcagg aattacagta
     7261 atgttagata aacaacaaac gaatcaattg aaaaattatg tttttgaaat gacaaaagag
     7321 gctattgatc aagcaagaat tgataccgga ttagaaagac catttctaaa aggaaaagag
     7381 atggctaaat atttgaatgt atcatataca acttttttga aatttaaaag aatgggattg
     7441 ccagttattt tattagaaaa aatggagttg ttttcaaaag aagaatgcaa aaaatggata
     7501 ttgagtcatc aaatttaaaa aacacctcgt tttttaaatt tgatgaagta gaaaaaatta
     7561 aaaagagtgt tcccgtcagt ttgcagatcg ggggaacact caataataaa gttctgagaa
     7621 aactttattt gaatctatta tagcaaataa aatcagaagc aggtgtgaca atgaatgaaa
     7681 aagaagttga aagcttagtt gaaaagcaag gctattctaa ttctgatata tatgcagaat
     7741 tatactatca atatcctatt tttttaaacg taaatgaaaa atataagaaa atgaaactaa
     7801 gttctaagtt agtttatatg ttactaaaag atagaaatag actttcattg aaaaataact
     7861 tgtatgatga gaatcaaaaa atttttttga tatttgaaat caaaaatctc gtagaaattt
     7921 cgggaatgtc aaaaaataca atcttatcag cactagatga acttcaaaaa gttaaacttc
     7981 ttgtgaaaag aaaaaatggc ttcaataaaa gtaaaatgca aatgttccca aatttttatt
     8041 atttgttaaa gcctgttctg tgcaaagatg atgtttataa aattaataag ataacccatc
     8101 aagaaaatag acaaaaaaac ttgatagttc aaaatttgaa ctatggaaca aatcaatatt
     8161 ctgttgatag ttcaaaattt gaactatgcg acacatcaat gaaaacaaga gacaaaaaca
     8221 attttgctga tagttcaaaa aatgaactag aattatataa gtcttataaa gatagtaaag
     8281 atattaaaga atcacataat gatgatcttc ttgaatcact ttcaactaaa aataataaat
     8341 tagatgaaga attgatcgaa acatttattg aagaaaatct tcttaaacaa atatggggag
     8401 aaagtattgt gaattgctta aaactggcta gcaatagtga ttatcgtcaa ttcgacaact
     8461 ggtacaaaaa attcaaatat gcgattagat ctgctgaaaa agaacaaaaa attcaactga
     8521 aaattatcta tgaaatttgt aataacaagt attttgtaga tcatgttaga gaacagctat
     8581 caatgacttt acgtgacttg atacgaagag caaaaacaga tcatcgtatc aaacaaaaag
     8641 acgattatat ctttgcttct ctaaaaaata aagcgttaga actaattgaa ttgcaaatca
     8701 gcgaaggaat agataagcaa taagaataga attgagtcaa atgaaatgga ggggtatctc
     8761 cacttcattt gagaactttg aatataggag gggttatttt gattaaaaat cgtttaatag
     8821 atcaagtttg gtgtctagat caaaatacag atagtctcga attaatcgag actatttgct
     8881 tcaacactat agttctcgat ttaagagctg aaaatgataa ttgtgtaact catgtcataa
     8941 ttaatcaaaa aatggctcag aagctctctg agagcctcag aaaatgggct aagggaggta
     9001 acgatgaaaa taattgaaaa aaagattgcc aataaaaaaa taaattggtt ggttgttaat
     9061 tttcttgttc tgatagctat gattattttt acaagtaata ttgctcatgc gagtattttc
     9121 aattggtttg atgacacaaa aagtcaaaca gagtttttga ataattctct ttatagacct
     9181 tacctaaaaa agtacaatgg agatttcctt agtcagttcg ggatttggct cggatgggct
     9241 gtggtaaaag gcatgtttac tgtgacagat tcgattcaaa atatgattcc agatgtgctg
     9301 gatttgttca atttcattga aagtacaggg ctaaataatg tttatcaatc agccgtaaat
     9361 acaattgtca ttggattaat gatcctttca ttgatgtttg ttggttacaa aatgattact
     9421 ggaaaaggaa cagtggattt aaaatcggtt ggaatgaacg tagtgatgtc tatagcatta
     9481 atacttttaa tgccaaccat gatttctagt ggtattcaat ttgctaaaac attttataag
     9541 gactctacta cgattacaaa ttcagatgac ggagtcgctt ggtcattgat aaaacaagga
     9601 gtaacagatt tagcatatat taataaaaca gatcagtact caagcattga taaaacggag
     9661 gatcgaaata atttaacacg aaaaaatttt caacagacgg atctcacaca agtgttaacg
     9721 gataaagtga ttgataaatt ggaaaaagaa aatccaattg cggacaatct tcgttatgaa
     9781 ttagttgaaa attctaataa tgaatttgtt gctacaaagt tttctgacaa cttcttatca
     9841 acattttctg atagcttgaa atcaggctat taccgatacc aagcaaatct atgggggatt
     9901 tcaattgggt tgacagcgtt agcaatcgcc tatgtgttca gcgcgtttgt gatcattaca
     9961 gcaatactag aacttgcatt taaacgtgtg ttaggtgttc ttgtttttgc aacagatatt
    10021 gagacaggac agcgttcaaa agttgttcta tcagatattc tacaatgtta cttaactgtt
    10081 gggtttcaag gatttgggtt atcaatgttt gctatgttta tcaattttct gaactcagga
    10141 caaggtatct caacaaatat ttttataaaa acaattgctt atatctgtgc cgtttttgtt
    10201 ttaatcaaag gcagtggaac agtcatgaga tactttggtg ttgatattgg actcaaagaa
    10261 ggctatgggc aacttgcttc tgcttttgga atgggcgcaa tgctgtttag aaaaggatca
    10321 aatggattta atcgtgctaa aggaagcgga gaaggagaag atcgaaaacc agaaaagaat
    10381 ttcggcgaaa ctctatcaaa aaaagctggc aaaactggaa gagctttggg atatgcccat
    10441 gaacgtggat tatccggttt agcttcagat ggagctacaa tggctagtga aagagcaaca
    10501 aaaccattta agagtatgcg tgatatagct aatgatacca agaataagtt taaagaaggt
    10561 ttagatgatg gtacagtgtc cgctattaat aaaaatagca aacctatgtt agctaaaaat
    10621 aaagaagatg aaactggaaa atatgctgat tccgtgccaa cacgtctttc agatagaaaa
    10681 gatggtgcta aagttgaaaa tgccgataga attatgtcta gttctgaacg tatgcgtgaa
    10741 gcaatgaaaa ataatgctga atcgaataat aatcctgcaa gcgcaattca gcaaaaagtc
    10801 caacaagata ttgaagaacg taaaaatgct atgcacggtc aagctaagtc tgctgaagaa
    10861 ttaattaatc agaagcgtca tgaagcgaaa tacacgcctg aagcaatgaa tcgcgaggaa
    10921 ataatgttaa ggaaacgtgt agaagggcgg acaggcgcta atattgacgg aaaagaagcg
    10981 ttgaccaaag aaagaattca agaagctaaa aattcaaata caggattaga aaaattagtc
    11041 aaagaaaatc cacaaaattc tacaagtagt catggtggcc gatcagtaga tgtccgagaa
    11101 aatattcaag gttcactgaa tggtcatgtt ggacggaatg ttgatgtaag agagaattta
    11161 caaagttctt caaatgctca aggtactaag acaattgatg ttcgagaaaa tctgcaaagt
    11221 agcagtacaa gtcaaccaaa aacagtagat gttcgagaaa atgtacaaaa ttctacaagt
    11281 agtcaaggat tcaaaacagt agatgttcgg gagaacattc aaaaagatag tggagaaatg
    11341 aaagaaaaaa cacaaaaaat caatatcgtt gaagacagaa agtcagcgaa aaaatttgat
    11401 tcaaatcatg aaacagtgat tattgatagt gaaatccgtg aaaatgataa gggatcaaaa
    11461 tcaagacgaa gattcactta tgaagacaat gagttattca gagatacact taatgatcct
    11521 aatccacttt tcgataattt attaagaaat aaaagggggg gattgtcatg caattaattc
    11581 taaagattag tacgcatgat ttcttagaac ttgacgaatt atgtgaatca gtaaagaacg
    11641 caacaaatga aaaaatcacg gtcactaatt atattgaact gatgattcaa gaagaattaa
    11701 agatgaaaaa tgaaatagag agcaatttca aatgttgctc tctagaaaaa aatgtgaggg
    11761 gataatcaat ggatgataaa caaaaaatgt acgaaaaaaa tcgcaaacat agtgaggcct
    11821 taaattttcg tgtcgataaa aagacttatc ttgaactgac attgattgca aaacaaaggg
    11881 gaatttctag agcagacgtt tgccgtgagc ggttaaaggg actcaaatat gaaccgccgt
    11941 tgttagctga agatgatagg gtagttttaa ccaaacagtt aaatcaaatt ggtaacaatt
    12001 tgaatcaagc ggtaaaggca ttaaatatca ttgctaaata ttttagagaa gaatctgaag
    12061 ataaaaaaga aaagagccta cattttagtt ttgatgctag ccgttacaac atttttaaag
    12121 atgaacttgc gaatagcata gcaaaaaagt tgatgattag ttatccagat gacatttttt
    12181 tggagtccaa tcggagtgcc ccctcttttt ttaaattatc agaagtagaa aaagaatatt
    12241 tcgaatattg gatagaaaag caaactagac cgggtacgaa agaagagtat tcccaaaaag
    12301 ttgatcggaa tttaattcac tcaattcaga ttattgcaag tttaaaagat gaggtgaaac
    12361 aactttggga gctactataa catcgaaagg acgcaagaaa aatttatcct cgctattaca
    12421 atatgcagct aatccttcaa aaaaagaaaa atgtgttgca gtctcaggaa taaattgtag
    12481 tgatgatagt gaggaagcac tgcaagaaat gaatttagta cgtaccaaga tgaataaatc
    12541 taaagatata gcacaagcgt ttcatgttat tcattcgttt gataagagta cttctaaaga
    12601 gttaagtatt gataaaatgc acgaaatagc tgtcgaattt gcagagaaag cttttccaaa
    12661 ttcgcaaatc attgttgcaa gtcacaatga taaagatcat tttcatagtc atcttgtcat
    12721 taataatatt aacatggaaa catgtaaaag gttgcgtatt gaccctaaag atttagaact
    12781 aatgcgaaaa attaacgatc aagtcctcaa tgaacatggt cttgaatcag tgaataatga
    12841 gtactatgaa aacttagaaa agagaaccaa tctttattca aacgatcctt ctaagatgaa
    12901 atatggaaaa attaattatc aagagtttgt gcaaaatgcg atcgaagcaa cgttggaaaa
    12961 atgcgaagtg aattcttttg aagatttttc ggatcatttg gcaaaagaat ataacattga
    13021 agtttatcaa ttttcaaaaa caaataacaa acttggctat gttctttaca aacaaggtgt
    13081 aaatttctat gagcgtagag gtgagttaat tactttagga agagataaac aatctagaaa
    13141 agaaaaaagt cagtatgtcg aacgtacttt tagtgcaaga aagttaggga aaagatattc
    13201 atttgaatct gtttctgaac aacttgaaaa gaatttagaa gaatcacata taaaaaattt
    13261 ttttaatatg gaaagagagc aagttatgtc gctaattagt ggagtaaatt acaaaactgg
    13321 tgagttaaat gtaccgtttg tggaattaga ttccatcgca caaaagcaat ttagcgaccg
    13381 tgaaggcgat gaaaaattca ttaaatatga tgacttcttg cgagaggtag acaagcattt
    13441 tgtaaataca atgtctttag aagatgatca agaatacacg attaaacttc atacggatgt
    13501 gaatcctcaa actttacgta gtgcgaaaag taaaacaatt tctattaaaa agactgatca
    13561 gaaagagtta tttctaccgg caaagggttt tgaaaattgt tctcatacac cttctgctaa
    13621 acagattcac tatcaaatca gtcgcataat gaatgacgat gtaaaaatgt atcgtgctaa
    13681 aaaggattat gaattcacac aacaaatgat tgaaaatcag caaaaaaggc aacaaaattt
    13741 tgaaatggaa ttctgaatgg gcttgttagt ggactttcca gttttttgcc tatgaagagt
    13801 tgcttggaaa gaagcagttt ttaggacata cgccagctac ccatctactt gccagtcaat
    13861 ccgccccgct cacgctaggc tactttctgg caagtcagat ggcgggtaaa tctctgaggg
    13921 agcaagctcc ctacagactc cctcccaaaa aaagaaaggt ggaagaaaaa aatgaactat
    13981 ccatatttta aagtctcggc ttcagaagaa actaaagaaa tattcaacaa tttttacaac
    14041 caaaacaaag gaatttttgg ttcaaaggca aatatgtttc gtgttatggt ttcaaattta
    14101 ccagtgttag ctagtccatc aaataataaa tttaatgatc ctgaaagcat taaatttgaa
    14161 caaaaaattt cagaactaga atcaatgata agtaatgaag taattgaaaa gctggatgac
    14221 attgatcaaa agttatcgta ttctctaaaa aacaaataca aaacggagga gaaaaaagat
    14281 gtttaaagtt tacttcccgt ttcagtggct cgttaaagga attcttttag tgctactagg
    14341 attgattctt tttggaagtt atctaacaat taaagccaga cattttccag aagaaaagcc
    14401 aataaaaatt gtaacaaaag atttaaaaat aggtgaaact tatcaggcag atggggttga
    14461 actaacattt aaaaatattg aagtgaccga aaaaagaaat gaatttgcac aacatgatcg
    14521 tgtattacaa gtgacctata cacttataaa tgatacagaa aatccgatac gtccacaaca
    14581 atggtcgatt gaaaataagg atggtgaaga actttatgag tatgatatga tattggataa
    14641 tccagagtat attgaaagtg gcaaagaaga aacagtaaat tactattatg aattaccgat
    14701 tgaaaggcag gactaccaaa tcgtagtacg tagagagaat ggttacatag ttgggttgcc
    14761 atttacttta taagttttat tttgttagta gtttttaagg taatatcagt tttgtgattt
    14821 ttgaaaatgg aactgggaga atggctgagg aaaataaagt tgttccgttt gaaacattag
    14881 tgataaataa ggaagagttt gagcaacttt ttcttgattt ttttattcaa tatgacatga
    14941 tttatgattt tagttcgtca ctagatttat ttgaattaga agcagaaata atagaaaaca
    15001 gtcgagagaa gaatggtaaa atcattattt cagtagaaaa attcaatgaa ttattctcac
    15061 aaacatttga aatagatcca gactattatc aaccacaaga agtttttcga gagtttagaa
    15121 ataagattat tggttttagt caaactacaa aatgaaagct taataaagca agtgttgata
    15181 gaattattcc tcaacgcttg ctttatttta tgggcacttc ataatagagg tatagagaag
    15241 agaggtattc attttggata ctattactcc aacaaacttc aaagaaaata taggtaaagt
    15301 gttgcaagaa gtagttcatt caaattctga aatagaaatt actagggatt ctaaaaatgg
    15361 attgaacgat ggcgtcatta tgatttctaa gagaaaatgg gagttgttac aagaagaact
    15421 ttatttacaa cgaacgggta catgggctta tgtatctgat tta
//
LOCUS       DAFEOM010000063        15230 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.066, whole genome shotgun sequence.
ACCESSION   DAFEOM010000063
VERSION     DAFEOM010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.066"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            278..787
                     /locus_tag="LP524_002141"
     CDS             278..787
                     /locus_tag="LP524_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL2235168.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="LP524_002142"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="LP524_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL2235169.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="LP524_002143"
     CDS             2322..3302
                     /locus_tag="LP524_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL2235170.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="LP524_002144"
     CDS             3424..4842
                     /locus_tag="LP524_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HBL2235171.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="LP524_002145"
     CDS             4913..6382
                     /locus_tag="LP524_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL2235172.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="LP524_002146"
     CDS             6392..6859
                     /locus_tag="LP524_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL2235173.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="LP524_002147"
     CDS             6895..7767
                     /locus_tag="LP524_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL2235174.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..10382
                     /locus_tag="LP524_002148"
                     /pseudo
     CDS             7848..10382
                     /locus_tag="LP524_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            10538..11311
                     /locus_tag="LP524_002149"
     CDS             10538..11311
                     /locus_tag="LP524_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL2235175.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11304..12095
                     /locus_tag="LP524_002150"
     CDS             11304..12095
                     /locus_tag="LP524_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL2235176.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12105..12596
                     /locus_tag="LP524_002151"
     CDS             12105..12596
                     /locus_tag="LP524_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2235177.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12614..13024
                     /locus_tag="LP524_002152"
     CDS             12614..13024
                     /locus_tag="LP524_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL2235178.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13028..14428
                     /locus_tag="LP524_002153"
     CDS             13028..14428
                     /locus_tag="LP524_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HBL2235179.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14451..14825
                     /locus_tag="LP524_002154"
     CDS             14451..14825
                     /locus_tag="LP524_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL2235180.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5286 a         2135 c         3047 g         4762 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaat
     8881 gtttcttaaa tcactttctg aaattgattt gattgaactt tttcaaaatc acatttcaat
     8941 tgaagaaagc atgagtcaat taagagatag aatacttaaa gagtgtgcag gttcttctga
     9001 gtataattat ttagaaagtg acgaatttat agcattaaga aactatgttg ttaataaaat
     9061 atcaccaatt attgattcga caggtctatg tttattagat gaattcttgg cggatatcag
     9121 tctatttata atgtttattg atgtaattga agaaacatca agaaattttt tgctgtctaa
     9181 gttcagaaaa tttcgtatga aaaatgataa acaaaaatta ttggcaaatg agatttggag
     9241 tagtttagaa gtcgaagaaa gtaaacaaga acttttgttg aaatggatac tttttcttgt
     9301 aaagaagttc tatgtgaaaa taccagaaac ccattgtctg atagttatgc acggtaaaat
     9361 aacagcttct gcggatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
    10141 atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg
    10201 aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat
    10261 ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta
    10321 ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct
    10381 aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat
    10441 ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt
    10501 gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt
    10561 agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc
    10621 ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact
    10681 ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc
    10741 agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca
    10801 ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga
    10861 tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa
    10921 agaagattat caaaaattag agtggatgca atatttggca tcaattaata caagctttgt
    10981 atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat
    11041 caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc
    11101 tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt
    11161 agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg
    11221 cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc
    11281 tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa
    11341 ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg
    11401 gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa
    11461 tgaaacaatc cgttttagct cacttacaat tttttaacac tacgccgtat acagcgcctt
    11521 acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag
    11581 taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg
    11641 tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt
    11701 ttgttggaat tattttgtgg atactggcat caattttatt aaggattact gcttatcctt
    11761 tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa
    11821 agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca
    11881 cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa
    11941 atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt
    12001 attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt
    12061 ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga
    12121 aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat
    12181 ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg
    12241 acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta
    12301 caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta
    12361 ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa
    12421 gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa
    12481 atattactga agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga
    12541 cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac
    12601 agaggatgag tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt
    12661 agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga
    12721 tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca
    12781 agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag
    12841 aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat
    12901 tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag
    12961 aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga
    13021 gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc
    13081 tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc
    13141 tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga
    13201 atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg
    13261 aagtattgtc caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc
    13321 taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca
    13381 ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga
    13441 ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg
    13501 agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga
    13561 agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat
    13621 tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg
    13681 tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc
    13741 agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga
    13801 aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa
    13861 ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct
    13921 tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag
    13981 ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga
    14041 ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa
    14101 attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa
    14161 tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct
    14221 gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga
    14281 tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt
    14341 cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta
    14401 ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa
    14461 atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg
    14521 aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt
    14581 tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag
    14641 ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaag
    14701 atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg gaaaatggaa
    14761 tccgttactt taatttctac ggaccaaatc atgagattat agagataagc caaatgaaga
    14821 aataaataga taacgaagtg ctctaagttg gtatgaacga aaagtacttc attttttggt
    14881 atcagcattt caatagttta atgagaattt actatttcaa attaattcaa agtgacagta
    14941 ttttttactt atttggtttc aaattttgta atccttccta tttattggaa tacactccac
    15001 attttgtata caagtcatat catgttttag caaagctctt accaagcaaa gttataatga
    15061 tgttttccaa agtttgatct ttcgatggag gctaacacat attttttgca ggacagccaa
    15121 aattagatgt atagaataga gtctgtgagg cgtatagaca gtttattgtc tgtatgcctt
    15181 tttttattta gaaaaatttt tagtattttt cgaaaaatgc tcactcctag
//
LOCUS       DAFEOM010000064        14839 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.067, whole genome shotgun sequence.
ACCESSION   DAFEOM010000064
VERSION     DAFEOM010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.067"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            417..1031
                     /locus_tag="LP524_002155"
     CDS             417..1031
                     /locus_tag="LP524_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL2235181.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            1804..3543
                     /gene="yfcC"
                     /locus_tag="LP524_002156"
     CDS             1804..3543
                     /gene="yfcC"
                     /locus_tag="LP524_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBL2235182.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            3700..4884
                     /gene="iadA"
                     /locus_tag="LP524_002157"
     CDS             3700..4884
                     /gene="iadA"
                     /locus_tag="LP524_002157"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBL2235183.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            4995..5198
                     /locus_tag="LP524_002158"
     CDS             4995..5198
                     /locus_tag="LP524_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBL2235184.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(5299..5961)
                     /locus_tag="LP524_002159"
     CDS             complement(5299..5961)
                     /locus_tag="LP524_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2235185.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(5974..7476)
                     /locus_tag="LP524_002160"
     CDS             complement(5974..7476)
                     /locus_tag="LP524_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2235186.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            8024..9703
                     /locus_tag="LP524_002161"
     CDS             8024..9703
                     /locus_tag="LP524_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBL2235187.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            9830..10309
                     /locus_tag="LP524_002162"
     CDS             9830..10309
                     /locus_tag="LP524_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HBL2235188.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            10528..11208
                     /locus_tag="LP524_002163"
     CDS             10528..11208
                     /locus_tag="LP524_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBL2235189.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            11216..12547
                     /locus_tag="LP524_002164"
     CDS             11216..12547
                     /locus_tag="LP524_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBL2235190.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            12562..13524
                     /locus_tag="LP524_002165"
     CDS             12562..13524
                     /locus_tag="LP524_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBL2235191.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            13635..13973
                     /locus_tag="LP524_002166"
     CDS             13635..13973
                     /locus_tag="LP524_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBL2235192.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            14052..14759
                     /locus_tag="LP524_002167"
     CDS             14052..14759
                     /locus_tag="LP524_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBL2235193.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4762 a         2578 c         3192 g         4307 t
ORIGIN      
        1 ttttttgtat tttcttgctt gcacttggat aagagattac ttacaataaa ttcagataga
       61 aacagctgaa taaaaaaagg ctccctgacc cttagttggc gctaagaatc agggaatgac
      121 cagtcacaca catacaccct gaccgatcat tttgccatag tcaatgcagt gcatcgacct
      181 aatttattgt acctaatcta gggtgaaaat tctaccccta tgtagatatt ttccatggtc
      241 tgcacgttgt tttatttgct atgtcaaggt attgggcgta ttcgttcaat gccttttttg
      301 tgtttgaaac agcaaagacc acatgtgtta ctgtctaact actgtttttt tatgtacttc
      361 cttagattag gcagctgttt ctattcatgt caagtatagc taggcgggag agataaatga
      421 gtttatgtta ttttgactat tatcaccaat tggaagagaa attaaggata gaagagccgc
      481 tgatgcttca agctggcact tggatcgcga aacaaattaa tcaaggtggt agattgcaga
      541 tatatgctag ccgcacacta agtggtgtag catttgaatt ttgggatcaa accccggatc
      601 ttgtaccaag tatattgatt gaaaatccag ccggaggtgt ctatgaaacg ctagaaggtg
      661 caggacaggc aattattgat cagctttctg tcaaatcaga agacattttt cttcttcttt
      721 cgaatgaagg aagaaacccg gcaatcatcg aactagctca gtggatcaaa gaaaatggtc
      781 atccattaat cattgttact ggatttgatt tgtcacgaag tatcaaatcg caacatagca
      841 gtggtttgcg attatttgaa tttgctgatt tggttttgga caaccacgcc aatttacatg
      901 atgcagtctt gactttgcca aatctagatc cagcgatctg cggaacagcc tctcttgcaa
      961 cgatcttttt gctgcagcaa gttttgtatt ttacagcaaa acagctagtc caggacacaa
     1021 gtggaaaata gttatccaca tgttgacaaa gttccttaaa aaaataatgt tccacataaa
     1081 aatggagcgt ttgaaaaaaa gaaaacgatt attaatgagg attaacaacg actacttttt
     1141 ttgttttagt tattttaaga tggatcatct ttgattttaa gcttgttttg tttaaagttt
     1201 gttattttta taagttgttt cacagttatg agaaatgagt tataagaata tgtagtgtca
     1261 gaaaaataaa aagtagtgga aaaagagaca agaaagaatc catatggatt ctttcttgtc
     1321 tcttaataca cgttgatgaa agagtcttcc ccaaaactct ttacaaagta attatagtac
     1381 atttgttatc attattgcaa tgaagtttac ctgatataac aaaattgtcc aacaggatac
     1441 ctttttctaa tagaaataaa atacacaaaa attgaaggat agataatgga tatttataag
     1501 tgtttattat tttttttgca atgaaattca atttttcaat gattgtaaaa tgaaaaaaac
     1561 aggacgagat tttctctaat ctaaaaataa aaaaagaatt ttaaggaaaa acagagaaat
     1621 aaaatactta atataaatgg aaagagaaaa aaataatcat acataggaga aaaaaaacaa
     1681 aatagaaaaa agtgattttc taataaatta ttcatgtgga aatgtcattt ttactgtata
     1741 attcatttga ttatccattt tttattcatc aaaattactg aaaaaagaaa ggaagaaata
     1801 aatttgaaga aaacaaattc ttcaggaaaa ccgaaacgcg acttcactaa attatcgaca
     1861 ccccatacgt atgtcattat ttttggtgta gtcatctttg catggatact gacttttgtc
     1921 gttccagcag gaaagttcag cacgcaagat attgaataca aagatgcaaa tggcgaaaca
     1981 agtactcgta ctgttttacg tcaagattcc ttccgttatg cttacgagct ggataagtcg
     2041 tatgtgtttg atcaattaga agaactgcaa gatcatccag cagaacgtga aaaactggat
     2101 gttccggaaa aaggattgga aaaagtcatt gctgacggag aaaaaaatct tacgcaggaa
     2161 aaactagatg aaatctcctt gacagatgat gtgctttacg atcagtacgg cgaaaatatc
     2221 tatgatactt ctaaaaaatt gcataaaaca gccaaaatat ggggaacaga tgattttggc
     2281 ggctttggtt tcttaaattt tgtttttgaa ggactagtat ctggagataa gtatggatct
     2341 gctgtaggga tcgctgcatt gatccttgtt gttggaggtg cttttggtat tattatgcgt
     2401 accggagcga tcgacgcagg tatctatgcg tttatcagta aaactaaggg actggagcgt
     2461 ttagctttac cgctattatt ctttgctttt tcattcggtg gcgctacatt cgggatggca
     2521 gaagaggtaa ttccattctc aatggtgatg gttccatttg tcattgcgtt aggatatgac
     2581 tcgatcgtag cagtaaccgt gacttatgtc gcatctcaag tcggaaatgc gacctcttgg
     2641 atgagcccgt ttagcgtagc ggtggctcaa gggattgctg gaatccccgt gttgtctggt
     2701 gcgacattcc gattgattat gtgggtagtt gttactgcat tagccgctgg ttatatgatg
     2761 atatatgcag aaaaaatccg taaaaaacca gaaagttcgt tgacttacaa atcagatgat
     2821 tatttccgtt ctcatatcaa aaagacatct gatgaaaata aaaaattcat gcttgggcat
     2881 aaattgatct tattagagat gttagttgtc cttgtctgga tcgtttgggg cgtgactcaa
     2941 aaaggctatt acattccaga aatcgcttct caattctttg tgatgggatt agttgctgga
     3001 atcattgctg ttcttttcaa attagatgga atgaaagtca atgacatcgc ttcttctttc
     3061 caatctggtg cagctgattt agctggtaca gcaatcgtag taggtatggc caaagggatt
     3121 cttcttgtct tgggaggatc tgatgcatct gttccttcag ctttgaatac gattttgcat
     3181 gggatcggga cggcgttgac cggtgttcca gctgtcattg gtgcttgggc gatgtacatc
     3241 ttccaaagtt tgttcaactt ggttgttaca tcaaattcag gacaagcagc tttgactatg
     3301 ccaatcatgg ctccactggc agatttagta ggtgtatcca gacaagtagc agttcttgct
     3361 tatcagcttg gttcaggttt tatggacgct tttacacccg tatcagccag cttgattggt
     3421 gtgctaggtg ttgcacggat cgaatgggca aaatgggcaa gattccaaat caagatgcaa
     3481 ggattcctgt ttgttttagg aactattttc atcatgattg ccattgcaat aggtctgcag
     3541 tagtactgga gacctgtaaa taaaaaagta atccaaacag tagataagga ctccaagaac
     3601 cgtcatttta cggtaaaatc aaaggagtaa agatcctatc gtttggatta ttttttaaga
     3661 tcctatcatc agttatactt aaagaaagag gaaaaatgta tgaaaatcat tcgacaaatt
     3721 gaagtgtttg caccagatta cttaggaaaa atggatgtac tgattgccgg tgggaagatt
     3781 ttggctgtag aagaacagct agaaggcggc tacgaaggag tggaagtaga agaactttca
     3841 ggtgagggaa aagtactaac cccgggtttc attgactgtc atttccatat tttaggtgga
     3901 ggaggagaag gcggttatca aaatcgaacg ccagaagtca cattgagcca attgactaca
     3961 gctggtgtaa cgacagttgt cggttgtttg ggaacagacg gagaaggtag agatatgact
     4021 gcgttgatca gcaaagcgaa aggtctggaa gcagaaggaa tctccactta tgtatacgaa
     4081 ggttcgtacc gattgccggt aaaaacagta acagattcta tcatcaaaga ctttttgaca
     4141 attgataaaa ttatcggaat cggagaaatc gcagtatccg atcaccgttc ttctcagcca
     4201 agttttgaag agttcgctcg tgcagtagca gatgctcgtg taggaggcat gttatccgga
     4261 aaagctggga tcatcaatgt ccacttaggc ggaggaaaaa gaaagctaga gctattgaca
     4321 cgaatcgtag aagaaacaga aattccgatc acacaatttt tgcctactca tgcaaatcgc
     4381 acaccagaat tatttgaagc ttgcgtggca tttgccaaac gtggtggcac gattgacttc
     4441 actgctagcg aagatcctga tttttgggag aagacagatg gtgaagttcg cttcagcaaa
     4501 gcactgaaac gattgatcga ggaagatgtt tctttagata atttcacgat gacctcagat
     4561 ggacaaggaa gcttgcctta ttttgatgaa aacaatcatt tcctagggtt aggagtagga
     4621 agtgccaagg cattgctagt agggatcaaa gaagctgtgc aaaaagaaag tatccctttg
     4681 gaaatcgcct tacgagctat aaccagtaat ccagcacgta tattgaaatt ggataaaaaa
     4741 ggaaaaatcg aaattggtgc tgatgcagat ctgtgtatct tggataaaga aacgcttgat
     4801 attgatacag ttatcgctaa aggagaaata atggtccaag aaaaagaagt caaagtatgg
     4861 gggactttcg aaaaaagttt ttaatcaaca gctctaacta tcttttttag aaaacgagcg
     4921 ttctgtttgt ctgtatctgt tgttgatgtt accctgaagg tagatcaata aacaaactaa
     4981 gaggaggaag cacaatggca gatttaaaag gacgttctaa agatatgaaa gataaagtag
     5041 tcggtgaagg aaaagaagca tacggcaaag caacagacga caagagcaaa gaaacagaag
     5101 ggaaagcaca atcactaggt gctgacatca aagaaaaagc tcgaaattta ggcgacgaag
     5161 tcaaagacaa gttttctcga aaagacgaaa aaaaataaaa accacttatt actaattgaa
     5221 tcaagcaaat aaaaaatccg aaccatatct gcgagacaga tatggttcgg attttttatt
     5281 tgatcattat cctgtttttt aaacttcgat tttttgaaat tttttatttt ttagttcaaa
     5341 gacttcatca cagctttttg ctacttcaat ctcatgagtc acgatgataa tacagcgatt
     5401 ttgttcatgg gcgatttctt ggaaaagttt gacgatatct tgtgaagttg tttcatctag
     5461 atttccagta ggctcatccg ctacgattag ttgatgttca cagcagatag cacgaacgat
     5521 agcgactcgc tgctgctgac ctcccgaaag tttggtcacc tgtttttttg ctaatatttc
     5581 atcaattcct acacgagcca gtcgatctaa agcaacttgt cgataattct cttgcttggt
     5641 cttagtgatt tccattgctg ctatgacgtt gtctagagcc gataaataag gaagcaagtt
     5701 gtaagcttgg aaaataatcg agacatcatt tttacgataa cttcttaacc cgattttgtt
     5761 cagtgaacga tcttgatagt atacctttcc ttcttttggt gcatctagtc cagaaataag
     5821 cgaaagaaaa gtcgtttttc cagaaccact tgctcctaaa atagcgtaca tttttccagc
     5881 ttcgaaattc agttctacat ctttataaag cgcatcttgc ggatcttgat accaaaatcc
     5941 caattgttct gtccgtaaca tcaaattcct cctttagttt aataggattt ttcttgggtt
     6001 taatcttaat atccctatag aagctaaaaa gacagaaaac agactgattc caaatgctac
     6061 tccggctaat agtcccaatt gtgcaggttt gacattgatt tccaattcgc tgacttgatc
     6121 agaaacagca aatggattat tcgtggttcc ttgttgacca tcgcgttgac cgccttgttc
     6181 ttgtctgtta cctggcattt gtccaccgcc aggagcttgc tggttttgtg tcgtggtttc
     6241 tgtctgttgg tcaagtagct gctgtcccac ggcattcgcg acgacgtttc cgctaagcga
     6301 agcaactcct aatgcaagaa tcatacaaat agccacttcg gtaaacaatt ggagaatgat
     6361 tttacttctg gattctccta aagatagcaa gacgcctaac tcatgccgac gctcccgaat
     6421 ggtaatcata atgatcagag taagtatgat aataccggct acagccacta gtaaaacaac
     6481 atttttagaa aaacttgcca cgttgtttaa aggttccaac attgactgat acatggaatc
     6541 atttgattgt aagctaaatg tatctgtgtc gatcagcttc tcagcttctt tgagaaactc
     6601 atccatttta tctggatcag atagagtgta aatagcagaa tctatcgtgt tatcgtcaga
     6661 tgtcccattt agttcatttg caaaagtgta agaagtgtaa agtgtattcg acggattcat
     6721 gaagttgaat ttcataccca tactgccgct tgtttcgctg gattcataaa ttcctttgat
     6781 cgtcatttca taagtggtat cttcatcttc agtactcgtg attgtgaaag tatctccgac
     6841 tgccaggtca tttgtttctg ctagagtaga atcaatgact acattgtttg tgcctttgtc
     6901 gctgtcatct attccttcac catccgtgat ttttgctgtt ccatcagaaa aattactatt
     6961 ttgtgcagtt gctgacacac cactgatttg aaagtctgct tgagtcattt gcggattccc
     7021 gcccattcct ccagcaggtt caccgcctgc atcagtagtt tctgaactgt cttcacttga
     7081 atcagaacta gtgatggcag ttattccatc ttttgccata gctgaggcgg aactttcaaa
     7141 ggaataactc tttacattat ctaattcagc tattttttcc gcgtccgtta aggaaactgg
     7201 tgtaagagaa aaactgcctg gatctggacg ttcttcagtg gtagaaccgc ttgaagaagt
     7261 ttgttcctgt tttttaaatg aagcttctcg attggcactt aaagtcactg ttgctccaac
     7321 ttctttttgt gcttgattcg ctgcttcttc tgcagcgctt cgaatcgtga ggccagctag
     7381 cacaaaaata agaatggctg tgaacacggc aaacaataac aatgaacgtc cccattttac
     7441 ttttgtactt ttccatgcac gtttcataaa attcatattt tgcatccctc ctagtagatg
     7501 catttgataa tagacaacct atcttaaagc tagcttaaat cttatgctgt ttgcacacca
     7561 tcaacatgag gttctgtata tataaaaagt cactatagtt attgcaaaaa atgtggtaaa
     7621 atgaaaaaag tatgtttcaa accgacgaag aagaaatgaa agaggaatag caaaagaaat
     7681 cctagatgct acagagggat ttttacgtgc atagttgttc taacaattgc tgatactagc
     7741 gatttagttt atttgttgta taataatggg gaacagctga atacttatat aagtattctg
     7801 gctatagcgt ttcgcaaaag atccattttg ttaatgaagt atatccaaaa taaaatgccc
     7861 atccaactgt cagaacgcgg atgtgctggt aattttcctg ctgattctcc ataagttgag
     7921 aaatagtgat tttgcatatg cttcattgga atggctcata tatttagata aagaagaaaa
     7981 tcgacatctt taaacttgga tgtcataaga agaggtgaag tttgtgagta ccattaaaat
     8041 tatcccatta ggcggcgttc gtgaaaatgg aaaaaacatg tatgtcgcag aagtgggcga
     8101 ggatattttt attttagatt gtgggctaaa atatcctgaa aatgagttgt tggggatcga
     8161 cgtcgtaatc cctgatttta cgtatttaga agaaaacatt gatcgtgtag ctggtgtctt
     8221 tttaacacat ggacatgcag atgcgatcgg ggcacttcct tacttgttgt caaaaatttc
     8281 tgtaccagta ttcgggacag agttgacgat cgagttagca aaattgaatg tcagtcgtaa
     8341 tgaagcagcc aaaggattta aggatttcca tgtggtagat gagcatacag aaatagactt
     8401 tggtgaaaca gtagtgagct ttttccgtac cactcatacg ataccagatt cagtggggat
     8461 caatctgaaa acaaaagaag gtagtatcgt ttatacggga gattttaaat ttgaccaaac
     8521 ggctatccca atgtatcaaa cagattatgc acgattggcg gaaatcggca aagaaggagt
     8581 cttagcctta ctaagtgaat cggcaaatgc agaaaatcct cagcctgtag cttctgaagc
     8641 ccaaatagca gatgaagtat ttgatacgat caaatattgg gaagggcgta tcatcgtcgc
     8701 ttgtgtagca agtaatttgc aacgagtgca acaagtattg gatgcagcag aaaaagcaca
     8761 acgaaaagtt gtcttgactg gtcaagattt cgaacgaatt attcgcacag cgatgcgttt
     8821 agaaaaactt caattaccaa gtgaagatct gcttgttacg ttgaaagaca tgaaaaacta
     8881 tgatccagaa gaactgatca ttttagaaac tggccgaatg ggtgaaccca tcaagtctct
     8941 gcaaaaaatg gcaaacggta cacaccgtaa tgtcaagatt gaagaaggag atttggttta
     9001 tatcactact acgccaagca tcgcgatgga aaccatcgtg gcgaaaacgg aagatatcat
     9061 ttatcgagct ggtggaacag tgaagttgat ctctgagaat atgcgcgttt ctggccatgc
     9121 caatcctaat gacttgcaat taatgatcaa tttgatgaaa ccaacatatt ttgtgccagt
     9181 tcaaggagag tatcgtgaat tagctgctca tgcagatttg gctcatgcag tagggatgcc
     9241 atacaaaaat atctatatta ctggacgcgg agacgtgctg gaatataaga acaagcgtat
     9301 gagtgttgca ggaactacaa gtgcagaaaa tgtaatgatt gatggattag gcataggtga
     9361 tatcggaaac attgttttac gtgatcgaaa aatcttgtca gaagacggga ttttcgtagc
     9421 ggttgtcact atcagtcggc gagaaaagaa aatcatttca aaacctcaaa ttacttcacg
     9481 gggattcgtt tacgtaaaag ctagccgtga tctgatgaaa gaaagcagca atatgattga
     9541 aacaatcgtt gagaaacatt tagaaagcaa tgattttgag tggagcaaat taaagcaaga
     9601 catccgagat caattaggcc gttacttatt tgaacaaaca aaacgccgcc cagtgatctt
     9661 gccagtcatt atggaagcta ctcaacgtcg aaataaaaaa taaacaaaaa gagtaagatg
     9721 aacctctcta tagagtgctc gtcttgctct ttttttgcga aaaacaaata atttttgaat
     9781 acagcatccc tcgctttata ataaacgata gaataaaggg agtgaatcaa tgatgagtca
     9841 aacaaaagtc gaacgttatt tagaagaaca aaagatcagc tatcaaccac ttgattttag
     9901 tcatgtggta gaagaatctt tcacagaaga actacaaaaa agagggattg atccagcttt
     9961 gatatgtaaa actctcgtag tgaaaggaaa caaaactggg gtgatcatcg cattgattcc
    10021 tttgaacgat cacttagatt acaaaaaaac gagacaagtg acgaaagatc gtaaagttgg
    10081 ttttcctgga atggattttg tattaatgca tacaggatat ccccacggag caaacacccc
    10141 tattggaata aaactcgcac atcctgatta ttgtttttta gcagacagtt ctttaaaaga
    10201 aaaagaagaa gtaattgtgt caagcggtga aatcgggaga agccttcgga taaaagtagc
    10261 cgatttagaa aagatcgtcc atcctattta cgctgattta attagttaag ggacgagaag
    10321 agaagctaag cagagcagag acctcaaact tgagattata tctgcttggt acttggtgca
    10381 tcctgtatta tctgttacaa ttgctttgta catactgtac tatcaatcca atgaaaatat
    10441 atttagcagc tatatttttt cattgatgtg caagtgatga aatcgttttt aataaaaaag
    10501 atacagagaa aacgcgaggt gtaaaatatg aaaattacgt atcatggaca ttcctgttta
    10561 acaattgaaa tggaaaatgg ccaacgcgtg atgattgacc cgtttattac aggaaatcca
    10621 ttgacagact taactgcaga agaagtaaag acagattggt tgctgattac tcatggtcat
    10681 agtgaccaca taggagatat gattcctatt gcaaaagaaa atcaagccac aattatcagt
    10741 attgttgaga ttgctgatta tgcgaacagc agaggagtag attcattcgg tatgaatatc
    10801 ggtggaaaac atgcgtttcc attcggcgtg gtaaaattcg tacatgcaca gcatagttca
    10861 agctatgaag tagacggtat cgttcagtat atgggcgagc ctagcggaat tattattcaa
    10921 gcagaaggaa aaacgattta tcatgcaggg gatactgctt atttcagtga tctaggattg
    10981 cttgcagaag aattcgatat tgatgtcgct ttcttgccga ttggcgataa ctatacgatg
    11041 gggccagaag atgcagcaaa ggctgcgcaa acaattcgtg ccaagaaagt agttccaatc
    11101 cattacaata ctttccctgt cattgaacaa gatcccgaat cttttgtaga gttactggaa
    11161 gatagtgaag gaaaaataat gagcgtctcg gaaacgctgg aagtgtaggc agaaaatggc
    11221 gacaaaacat gatttgattt tagaatatat tgaaagttta cctgttggca atcgaatttc
    11281 tgtaagaagc atcgcaaaag aattgaatgt cagtgaagga acagcgtacc gtgcaatcaa
    11341 agatgcagaa aatgtcggac tagtttcaac aattcaacga gtagggacca tccgtatcga
    11401 acgcaaatta aaaaaacata ttgaacgttt gacatttggc gaggtcgtca ggatcattga
    11461 aggggacgtt ttaggcgggg cggcaggatt agacaaggtc ttgaataaat tcgtcatcgg
    11521 cgcgatgact aaacatgcca tggaacgcta tattacgcca ggatcactaa tgatcgtcgg
    11581 caaccgggcg gaagtccaaa aattagcatt ggaagatggc gccgcagtat tgatcacagg
    11641 aggattcaat acgactgctg agattgcaaa gctagctgat gatcttgaaa tgccgatctt
    11701 acgcacctct tatgatacat ttacagtcgc aaccatgatc aaccgagcgt taagcgacca
    11761 attgatcaaa aaagatatca tgcttgtcag tgatatctat atgccagctg aaaagactca
    11821 ttatttgcag cagacagata cgatttctga ttatcagcgt ttgtcagaag aaacgcagca
    11881 ttcgcgtttt ccagtcgtca accgccatca tcgtttgatg gggattgtta cggcaaaaga
    11941 tgttttaggg aaatcgccaa accagatcat cgatcgggtg atgacgaaag aaccaatcag
    12001 tgtaaagaaa acaatgagta ttgcctctgt tagccatcaa atgatctggg atgggctaga
    12061 agtcatgccg gtcgtttccg atgacttgac attggaaggc ttgattaccc ggcaagatgt
    12121 aatgaaagcg atgcagcttg tccagcgaca accgcagatt gcagatacga tttcggatca
    12181 gatatctgga aatatccata cgattgatac tgaccgtgaa ggaaatcgtt tggaaaaccc
    12241 taagttcaaa ttcgtagtag caccacaaat ggtcaatggc gttggaacca tttcttttgg
    12301 cgtacttagc gaaatcattt ctgatgtcgc acaaaaaaca atggtgatga accaaaaacg
    12361 aaatatcttg attgaacaag tgaacctaca ttatttgcgg ctgatccaat tggaaagcga
    12421 attagacatt cgtccacgtg tattggaaat cggcagacgt tccgctaaac tggatatcga
    12481 agtgtttatt gaaaacacca tcgtttccaa agcaatcgtt gtttgtcaag taatggaacg
    12541 ttcttagaaa gcaggagtga aatgacagta cagcaagaaa tagtagatac aataaaaaaa
    12601 tacgataaaa tcattatcca caggcatttg cgccctgatc ctgatgcatt aggatcacaa
    12661 gtaggattag ccgaactatt acgggaaagt ttcccagaaa aagaaatacg tcaagtaggt
    12721 gaaacgattg aagggcttcg ctttttagca gaaatgcaag aagttgaaga tgtcttctac
    12781 caaggagcgc tagtcatcgt gactgataca gctaattcac ccagaatcag tgatcaaaga
    12841 tatcagttgg cagaaaaact gatcaaaatc gaccatcatc ccaatgatga cccttatgga
    12901 gatcttgtgt gggtaaatac aaaagcaagt agctgtagtg agatgattgc agaatttgct
    12961 ttgatgtttc ctgaatcttt aaagatgaac gttaaggctg cacggttact ttatgcagga
    13021 atcgtcggag atacagggcg ctttttgtat ccctccacga cgtcccgtac attagagatt
    13081 gcttcgatat tgagaaatta tccatttgat gcaagtgctt tgaacagaga gattgaacaa
    13141 atgccgatga aagtcgctaa actttccggt tatctttatc aaaatatcca ggtggatgaa
    13201 aatggtgcag gaagaataat ccttccgcaa gaaattcttg ataaatatgg aatcgatgat
    13261 tcagagacag cttcgatcgt ttcacttcct ggagtgattg acgaagtgct ggcttggggg
    13321 atttttgttc agcagccttc agggtattac cgcgtacgtt tacgttctaa ggggccgatc
    13381 atcaatgaat tggctaaacg ccatcatgga ggaggccatc cattagcaag tggtgcgaat
    13441 gcaaaagatt tagcagaagt agaggaaatc tataaagaga tccaagatat atgccaatcg
    13501 tataagagca gaaaaacaga atgatacttc gttcgaaaac gtgccttttg tagtaaaaat
    13561 cacaaatgta tcttttcaag atcttctttt tccgttacaa tagtgctcac taaagatttt
    13621 aaaggagaaa gaagatgaca ttaccaaatt gcccagaatg tggctcagag tacacatatg
    13681 aagatcgagg cttgttgatt tgcccagaat gcgggaacga atggaacaaa gaagaacaag
    13741 aaactgtcat agaagacgga ttagtggtat tagatgcaaa tggaaaccgt ctgttagagg
    13801 gcgatagcgt gacagtgatt aaagacttga aagtaaaagg tgcgtcaggt gccattaaac
    13861 aaggaacaaa agtcaagaac atccgtttag tagaaggcga tcataatatc gattgcaagg
    13921 tagaaggatt tggaccgatg aaactgaaat cggaatttgt gaaaaagaat taaagaatat
    13981 tcaaggtctt ttcacattct tttcctacta ctaatgataa aataaagtaa acgaaaagga
    14041 gtgaaacgaa aatgtatggt tatggaatgt atggtttttt agatccgaca tttcttttgg
    14101 tcattcttgg attagttatt tccggaattg cgtcagcgta cgtcaatagt actttccgaa
    14161 aatacgatca agtgagaagc aaaaacaatg tgactggtac acaagcagca caatatatat
    14221 tacaaagtca acagataaat aatgtcggtg ttcaacaaat tgccggagat ctcacagata
    14281 actacaattc aggtaataag atgttgagtt tgtctcaagc aactgcacag tctacttcag
    14341 ttgctgcgat aggtgtagca gctcatgaat gcggacatgc ggtacaagat gcttcgggtt
    14401 atgtcccatt gaagattcgt gcagcacttg ttccagcagc taactttggt tcgatgattt
    14461 cgttcccctt gattatggta ggtgtattgc ttagctggaa ctctacgtta atcaatatcg
    14521 ggattttcgc tttttcttta gcattacttt tccaattggt gacattacca gtcgaattta
    14581 acgcgtctcg gcgagcaatc caaattttga gtgaaggcgg actactgacg gaagaagaag
    14641 taccgatggc aagacacgta ttatttgcag cagcactgac ttatgttgca gcagcgcttt
    14701 cgacattcct gcagctgttg cgcttgatct tattgttcgg cggcaaccga agagattaaa
    14761 ataaagaagt tgtgaaatca gcgctttgat ctgagctaat accgagagtt agctgattga
    14821 ataccattta tttggaata
//
LOCUS       DAFEOM010000065        14496 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.068, whole genome shotgun sequence.
ACCESSION   DAFEOM010000065
VERSION     DAFEOM010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.068"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            136..456
                     /locus_tag="LP524_002168"
     CDS             136..456
                     /locus_tag="LP524_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235194.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            908..1108
                     /locus_tag="LP524_002169"
     CDS             908..1108
                     /locus_tag="LP524_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL2235195.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1255..4044
                     /locus_tag="LP524_002170"
     CDS             1255..4044
                     /locus_tag="LP524_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBL2235196.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4091..4618
                     /locus_tag="LP524_002171"
     CDS             4091..4618
                     /locus_tag="LP524_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HBL2235197.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4639..5829
                     /locus_tag="LP524_002172"
     CDS             4639..5829
                     /locus_tag="LP524_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBL2235198.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5869..7167
                     /gene="asnS"
                     /locus_tag="LP524_002173"
     CDS             5869..7167
                     /gene="asnS"
                     /locus_tag="LP524_002173"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBL2235199.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8343..9017
                     /locus_tag="LP524_002174"
     CDS             8343..9017
                     /locus_tag="LP524_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBL2235200.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDNIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            9014..9355
                     /locus_tag="LP524_002175"
     CDS             9014..9355
                     /locus_tag="LP524_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235201.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9385..9744
                     /locus_tag="LP524_002176"
     CDS             9385..9744
                     /locus_tag="LP524_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBL2235202.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            10269..12353
                     /gene="cadA"
                     /locus_tag="LP524_002177"
     CDS             10269..12353
                     /gene="cadA"
                     /locus_tag="LP524_002177"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBL2235203.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
     gene            12644..14110
                     /locus_tag="LP524_002178"
     CDS             12644..14110
                     /locus_tag="LP524_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL2235204.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4800 a         2510 c         3102 g         4084 t
ORIGIN      
        1 agaggttgtg acgtggtcac gacctctttt tgacgattat gatacaaaaa acatcattta
       61 tgaacaaaat gtgaataacg ggcgttcttg agatatgatt ttaattggga aaaacttatc
      121 gataaggagg tctttatgaa aaaaagactg ttatggtgtt tgctgtttct atccctaagt
      181 ttaacaagtc agccagtttt tgctgaagaa acacagcggt ctcctattac aggcacacgt
      241 ctgtacaaaa atgtttatgt acagacaaca gatcttgatt ctgcgattcc tgaaacagaa
      301 gaacggtctt tgcagccaag atttggttac ttttggtact gtccgacctg tgattatgaa
      361 agttgctggc atttcttaca ttctacagct gcagcttttg cttttactca tactcagaaa
      421 tatgatggac atcatcctgt tatttatgag gtatagatat gagcaaaaaa gaaaaactaa
      481 tctcgttgat cgtattggtt tgccttgttc ttctggtaac tgtcataggc tatcagaaat
      541 accatgagaa catcagacct actaaagaat cttccccagc ctcgaccgtt gaaacagaac
      601 cgataagtag tcagaaagaa taattttttt agtttaaaga agaacatcag agcaaaaaaa
      661 caccaactga acaatatgtc gtttatttta aaaaaagttc ggtttttcac tttatgtaat
      721 cgctgagaaa acttttttta ataaaaaaag tcgaaaaact ctagcttttt tgtaaacggt
      781 atgatatact ataggagtag tttttgttcg gtaatctgtg gtagcttgtt tcaaataaac
      841 atcttccaga tatacaccat tcaagtaatt gcaaaatatg tgttgataca caaggaggat
      901 ttttattatg gaaacaggta cagtaaaatg gtttaactca gaaaaaggat ttggattcat
      961 cactgcagaa aacggaaacg atgtattcgt tcatttctca gctatccaag gcgacggatt
     1021 caaaacttta gaagaaggcc aagcagtaac tttcgatgtt gaagaaggcc aacgcggacc
     1081 tcaagcaaca aacgttacta aagcttaatt aacctatact aaaaaataaa cgcccagaca
     1141 tttgcgtgtc tgggcgtttt tgtctatttt ctttaaaaag cgatgagagt atttttctgc
     1201 actttgtagt ataatttcaa aagaacatta gaggcaggag aagaggaaga tattttgaaa
     1261 ccaagaactt atgcagtcgt tgatatcgaa acgactggga cgaatccaaa agaagatcgg
     1321 atcatccaat ttggctgcgt catgattgaa tcaggtcgga tcacttcacg tttttcgatc
     1381 gatattcatc cagggcggaa gatttcaaaa caaatccagc atttaacagg gatcacgaat
     1441 caacgcgtcc aaaaagcgcc ttatttcgaa gatgtcgccc aaacgattta caatcttttg
     1501 gcagacacca tatttgtcgc gcataatatt tattttgatt ataatttttt gaaccatgaa
     1561 ctcatgcgat gcggacttcc tagtttgaag ataccaggca tcgatacagt agaacttgca
     1621 caaattttct tgccgacgga accaagcttt cgcttagctg atttgtcaga aagccttgga
     1681 tttattcatg agaatcctca tcaggcagat agtgatgccg aagtgacggg tcaacttctt
     1741 ttgctgatcg aagaacggat gagaaaattg ccaatcatca cgttagagca gattgcgcga
     1801 ttaagtagac acacaggtat ggatacgagt cgtttcattt atcatgtgat tgaagaaatg
     1861 aaagaaaaac cagagccact tgatccctca ttagaaatcg tcgatgggct agctttgcag
     1921 aaaaaagaag tggaactatt cacatccgtc cattatggcg agcggacata tccaagaaaa
     1981 aaacaagcaa aagaaaaaat gtttggtaaa acactgatgt atcgaaaaga acagaacaga
     2041 ctgatgaatg cggtctacga ccatttcaca aaagatgaat caaaagattt gatgatcgaa
     2101 gcagctacag gaatgggtaa aacaatcggt tatctgctgc cattgagtta tttggctaca
     2161 cctgaaaaac ccgctgtcat cagtaccgtt tcgctagttt tgcagcagca gatcatagaa
     2221 aaagacattc cattgctgaa tcaattgcta gatcagccca tacaacctgt aattatcaaa
     2281 agctatcgcc actatattga tttgcagcgt tttaaaggca cacttgtgga accgccagaa
     2341 caaaaacaat atgctttgta tcaaatggcg gtcttagttt ggctgacgca aacgaagact
     2401 ggtgatttgg atgaattaca tttaacaaat ttgaaccatt cattctttgc agatatcgcg
     2461 cacagaggta ttggattttt agcaagaaat cagtcatttt acgaacaaga ctttgttcgt
     2521 ttcttgcaaa agaaaatcag gcaaagtaat tttttgattg tcaatcatgc gtttttagta
     2581 caagaaacac agcgaaaaga accattgctt ccagctagtc catttttgtt gatcgatgaa
     2641 gtacatcatt tgccggaaat cgctgaaaaa gtaaatgaca aacgattaag tattaactat
     2701 ttccataaac aaatccagca attagaagaa ccaaatcagc tgtttgaccg aatacaaagc
     2761 tatttaccga aagaacatga agccttacgc tggttatcga tttatcgaga agaactttct
     2821 gcgctatgcg aagcacagat ttgtatggca gaaggattaa tgagtttagt gagcggcaag
     2881 caacctaacg gacagtatcc agaagaagtc atgatgacaa aagagatgtt ggatcagctg
     2941 ccactggaat ctgggacagc cattgatcac ctgcagcttt tttatcagga gatccagcag
     3001 ctacagtcac aaatgcgtct gacttttgag attatccaag aaagctggac aaaccagatg
     3061 cggatcgatt tagcaagttt atatagccta tttgatcaat tagacaagca atacttgctg
     3121 atggatcaat ggctggaaga ctggcgagat catttgatcc attggttcgt tcctgggaac
     3181 acagcgcagc agatgatttt tcatctccat gattttcaag cggctatgtt gccgtcgact
     3241 gtttggtatc ctcgatatga tcgtattctt tatattggag gaacgctgaa gatcggccct
     3301 aatcgacact atctctctaa gaggcttggc attgaagcag cacctcttaa agtgattgcc
     3361 tctccttatg attatgagaa acaggcaagg atctatgtgc caaaagaggc acctgccgtc
     3421 catcaagtca gcaatcaagc atatatcgat tatttagaga cagcacttac aggtctgatc
     3481 gaacaagaag atcggccaat acttgtgtta ttcacttctc atgatgtttt gcagaaggtc
     3541 tatcagaaaa tgcatctgtc tatgctagaa aaaggacgag aaatactagc tcaagggttt
     3601 ggtggaagcc gggagaagct tttgaaacgc ttcattttgt cagaaaaaag cgtattgttt
     3661 ggcgctgaca gcttttggga aggtgtagat ttgcctggag atgctttgca gatcgttgta
     3721 gtcacacgtt tacccttcga taatccgctt cgcccgatgg tcaaagctag gaacgcttat
     3781 ttagaatcag aggggatcaa tcctttttac caggaatccg tacctaaagc agctttgaag
     3841 ttacgccaag cattaggtcg cttgataagg acggagacag atcgtggcgt attgcttatt
     3901 ttggatcgaa gactagtcac tgcaaaatat ggcgcacgga tcatccatgc attaccgaaa
     3961 aaagtaccga tcaaagagct agctatggag gaaatctatc aggatatcca tgaattctta
     4021 aagaaaaatg aagaaggaag ttagaggagc tgtctttttt cataattaaa atctgctata
     4081 atgaaaagga atgaagataa aggagcgacg acccttgaag aaaaaacaga caaaggaaaa
     4141 aacgattaat cgcgtgttac ttggcttgat cgtattttta ttagcggtca tcgtgatcag
     4201 ctcgattttt tacttgcgtt ccagtcgtcc ggcggtgcag gcgaagaaag aagcagtaga
     4261 aatcgcaaag aaatatgcaa atcttgagga aaccgatcat ttttattggt ttactcgaaa
     4321 gaagacatac ttttcactga caggtacaaa tgataaagga aaagaaatcg ccgttatcat
     4381 tcctaaatct ggtgaaaaag taaaagtatt ggatcaaaca aagggactga ctgaaaacga
     4441 agcaaaacag acaattgctg cggcccatcc tgaaatccaa atcgaaaaag ctgctttagg
     4501 gatgtatgat gatcagcctg tgtgggaagt agtaggaaaa gaaacaaatg ggaaactgaa
     4561 ttattacctg attggctttg aatctggaga tgaagtcaag acaatcaagg gaatttaaaa
     4621 ggtaaaggaa tgaatgggat ggagttatca aaaagaacaa aacgattaga accgtctgtc
     4681 actttggcag ctgcggcaaa ggccaaggca ttaaaagcaa aaggcagaga tgtgctgagt
     4741 ttgactgtgg gagaaccgga ttttgcgaca ccagaaaaca tccaagaagc agcaatcgaa
     4801 gcaatccgca atgggaaagc tagctattat acaccaacag caggaatccc agagcttcga
     4861 caggcaatcg ttgattatct gaagaaaaac tatggacttg tctatgaacc ttcacaaaca
     4921 gtcgtaacag acggagcgaa atttgctttg tatacccttt tccaaacgat ccttgacccg
     4981 caagatgaag tcatcattcc tgtgccttac tgggtcagtt acggtgagca agtaaaactg
     5041 gcagaaggac ttcctgtctt tgtgaaaggg gaagaatcca atagctggaa agttactgtt
     5101 gaacagctag aacaagcacg ttcagaaaaa acaaaagccg taatcatcaa ttctccatcg
     5161 aatccgactg gaatgattta tagcaagaat gaactgcagg caatcggtga atgggcagta
     5221 aaaaatgata ttttgatagt tgctgatgat atttatggcc gtttagttta taatggcaat
     5281 gagtttactc caattgcaac gatttcagaa gcaatcaaaa atcagaccat catcatcaac
     5341 ggcgtgtcta aaacatatgc gatgacaggc tggcgcattg gttatgcggt gggaaatcct
     5401 gtgatcatca atggaatgat cgctgttgct tcccaatcaa ctagcaatcc gacagcagtc
     5461 agtcaatatg cagctgttga agcattgaaa ggtgagcaag atacagtaga agaaatgcgg
     5521 aacgcctttg aagaacgcct gaatacgtta tatccgcttg tggcagaact gccgggtgtt
     5581 tctcttgaaa agccgcaagg agctttctat ctttttccta atgtaaaaga aacgctacgc
     5641 atgtgtaagt atgaaaatgt cacacaatgg gtagaagatc ttttagaaga aacaggggta
     5701 gcgcttgtga ctggcgaagg ctttggggca ccagaaaatg tccgcatgag ttatgccact
     5761 gatcgcgcta cgttagaaga agcggtacgc agaatcaagc aatttattga gagtaaaagt
     5821 caaaattaat cctgaagaaa ttcaaggaat ataaaaggag agtcatacgt ggaacaaatt
     5881 caaatcatcg attcgaaaaa ccatgtggga gaaacagtaa caatcggcgc ttgggtcgca
     5941 aataagcgat caagcggaaa aatcgcattt ttacaattaa gagacggaac agcctttttc
     6001 caaggtgtag ttgtaaaaag cgaagtgtcg gaagaagttt tccaaacagc taaaaatcta
     6061 caccaagaaa catccgtgtt agtaactgga gaaatccgtg aagacagtcg ttcaaaattt
     6121 ggttatgaaa tcggaattac aaatgtggaa gtggtaagcg agagccacga ttatccgatc
     6181 acaccaaaag aacacggaac agatttcttg atggatcatc gtcatttatg gctgcgttct
     6241 tcaagacaac atgcgattat gcagatcaga aatgaaatca ttcgtgcaac gtatgaattc
     6301 tttaatcaac atcattttgt caaaatcgat ccgccgatct tgactggttc tgcaccagaa
     6361 ggcactacgg atctattcga aacaaattac tttgatcaaa aagcttattt atctcaaagt
     6421 ggccaattat acatggaagc tgctgctatg gctttcggaa aagtcttttc attcggtccg
     6481 actttccgtg ctgaaaaatc aaaaacacga cgtcatttga tcgaattctg gatgatcgaa
     6541 ccagaaatgg ctttcactca tcaagaagaa agtttagaag tacaggaaca atatgtagca
     6601 ttcttagtac aaaatgtttt agatcattgc gaatatgcat tagatgtact aggacgagac
     6661 aaagaagtct taaaaactta tacaaaattg ccattccctc gtattactta cgatgatgca
     6721 atcgaattac tgaagaaaaa tggatttgat gacatcgaat ggggcgaaga ctttggttca
     6781 cctcatgaaa cgttcattgc gaattcattc gaacagcctg tctttatcct aaactatcca
     6841 aaagccatca agccattcta catgaaacca catccaacaa gagaagacgt ggtaatttgt
     6901 gcagatatga tcgcaccaga aggttacggt gaaattattg gcggaagcga acgggcaaca
     6961 gactatgatt tcttgctaga acaaatccgt aatcacggtt tggatgaaaa agaatattca
     7021 tggtatcttg atctacgtaa atacggttct gttccgcact ctggttttgg tctaggcctt
     7081 gaacgaacag tcacttggat tgctggtatc gaccatgtac gtgaagctat tccattccca
     7141 cgtttgttga accgaattta tccataagag gtgatgaaaa agctgttctg catcaagtaa
     7201 ttagaacagc caagcaaaaa cagctgttca tgtttttgaa ttggcgggac ttattaccgc
     7261 agatgtaagc ttttgaatac cgtttagtgg agaggttgtg aaaaagctgt ttagtttcga
     7321 gtattaagaa aaattttgtg gaaatggctt tccacatttt tgcataaatt tggcttaata
     7381 ccgagaaact ggcttttgaa caccgtttaa ggaagaggtt gtgaaatcag tgccttgacc
     7441 tgagtattag agaaacaaaa cggaaaaata gcttctcata ttttaccgtt tttttagcta
     7501 atatcgaggg tcagctgatt gaacaccgtt tatatcaaga ggttgtgcgc ctgccgttca
     7561 gctccgagtc acaaggagta atttcaaaaa acagtttttc atgtttgatg actattgtga
     7621 cttgtgaccg aggagttggc cggagagcaa cgtttattta aaaaaaagaa ctgcgaactc
     7681 tcgcagttct ttttttaaaa tacgggataa aggaagtttc cagcagacaa gagaagaaag
     7741 caaaagaaat tgctatttga ttcacaaggt tctataacga aatcgcaatc aatcaaacat
     7801 atacaaagaa ggttattcaa atggaaatct gatttgatga tgcttctgct gttgaaataa
     7861 agcagcatct agtacctgaa aacaaactaa tagtaacatt cgaagatgga gtaggtccgt
     7921 attttcagca tgcaatgatc catatgtaag tttaattcat ccttgaaatc atcggaccag
     7981 ataaagttat gaccaatcta tcccaagcaa tctagaagag atattagtaa aagaacattt
     8041 actggaagtc ttagatgagc ctataaatat atgttctgat ggcgtcatca gtggtctgat
     8101 tgacggaagc ttaggtgtga tcgatttctc tggaccaaac aacgaaggaa tcaaggggaa
     8161 tccggcacgt taagaaaaaa agtataaaaa actagacact tgatgagcgt tcattcaatt
     8221 gattgaaggg actaggctgg tgtcattttt tatacatata ttttctcttt cgtcttgata
     8281 gggcttatgg gataatataa ggaagaaaat catcgtttta ttcaaaaagg agtttttgac
     8341 aaatgaaaaa acgtatatta tttatcggtg acagcattac agacgcagga agagaccgag
     8401 cagaagcaac tgatctaggt aaaggttatc cacttttgat tgccggaaaa ctaatctccc
     8461 aatatccaac gatgaaattt gaatgcatga accgtgggat aggaggagac aatattgcag
     8521 atctgcaagt acgctggcaa gaagattgtt tagctttaca acctgactat gtatcgattt
     8581 tgatcggaat caatgatacc tggcataata ctagcgatca ggctatattc ggaactgaga
     8641 aatctgcgca gcaatttgaa tccgtttatc gaaaattact aaatgaactt caaacagcgg
     8701 aaatctgcaa catcatcttg atggagccgt ttgtacttcc tataaccgac gaccgtcatg
     8761 gctggcgtaa agacttagac cctaaaatcc aaattgttcg taaacttgct aaggaatatg
     8821 gcgcattgct ggttccttta gatggcatat tcaatgcttt agggattgct tatggttttt
     8881 cgaaatatac aagagaagat ggggtccacc caacgattgg cggacatgag atcattgcac
     8941 aaagctggat agatgcaatt gccgcagtcg atttttttag tggagataaa aatgagcgaa
     9001 agaaaagtga gaaatgatgg aaatacggtg ggcagtaaaa gaagatttac ctcaaatcaa
     9061 tcagctgatt actgatcatc cagaatcatt cgtaaaagca aaaacggcag ctgacctaaa
     9121 tgataagaat aaactattgc tcgttgctgt aacaaaagaa aaacggatta tcggatttgc
     9181 gcaagcggac gtgcaacagt cagaagaacg ggtggatcag cgaccatcag gaaaactaat
     9241 ccaacacaca ttagtaacaa aagaaaatac ggaagaaatc caaaaaaaat ttgcagaata
     9301 cttgatgctg gaagctaaaa aaagaagaat aaaatcaatt tttgcccagt tttaatcaaa
     9361 aacagtaata gaaggagaaa tacaatgagt tgtttaggaa atgtcatctg gtttatattc
     9421 ggcggactaa tcggtggtct tagctggtta gcagcgggga tactttggtg tatcacgatc
     9481 ataggtatcc caatcggtat gcaatgtttt aagatcgcct ccctttcttt tgcgcctttt
     9541 ggcaaagaag taatttatca ccagtctggc atgtcattga tcgttaatat catctggctg
     9601 atcgtttcag gcctaccaat tgctctcgca catgtcgtca gcggcatcct tctatgtatc
     9661 acgatcatag ggataccttt tgccaagcaa tctttcaaac ttgctcgatt ggcgttgatg
     9721 ccattcggtg cagaaattgt ataaaaagag aataataaag gttagaaaac gcatagagtc
     9781 aggtgggata catgtatttc acctgactct atgtgttttc ctgtataagt tctcccatgt
     9841 gacttttacg tcttttattt atatgggatt actgtttatg gaaagttgaa ataattttca
     9901 ttaaagaact ctttctatat ttttgaaagc gtagtggagc ttcgaatatc tgggaaggac
     9961 attttgcagg gattgaaaaa agatattgtc ctatcgaaaa attaatgaat ttagacaaat
    10021 tgccagcaac aggcttcaaa cttagttgct atcctgtcaa gatcaagaag gcaagtgcgg
    10081 gttggataag agcaggggca atgatcgaag agaaaaagaa agaataagcc aaaacatctg
    10141 tatggaagaa attttccgat gcagatgttt ttcatttttt ctttcggata ccttgacata
    10201 tgaataacca ttcatttata attagttatg aagagatgtt catatgttga ggcgaggagg
    10261 tcattaaaat gaaaagttac agattagaag gtttagattg tgcgaactgt gcaatgaaaa
    10321 tcgaaaaagg agttcagaag atcaatggag tcaaggaagc aacggtaaac ttcacttctg
    10381 gaaaactaac gattgacgca gaggaagatc atttggctac gattgaacaa gaaacaaaaa
    10441 aagtagtgaa ggaattagaa ccagatgtca aagtaacaga aatagataag gaaaaagtat
    10501 ctgaacatgg aaatgaaaaa gagagaaata cgctgttccg gattttattt tcccttgcag
    10561 gtatcgcttt attgctatta tttgatttta atgaaccaat tcgcttgata gggtatttat
    10621 tgatttatct gctgatagga tatgatgtag tgaagaaggc agtgatgaat atcgtcaaag
    10681 gaaaaatctt tgatgagaat tttcttatgt ctgttgctac gattggcgct atgttgatcg
    10741 gtgaatatcc agaagccgta gcagtgatgc tattttatca aatcggcgag tatttccaag
    10801 gattggcagt cagtcattca agaaaatcga ttcgcgaatt gatggcgatc cgtccagaaa
    10861 cagctcacgt gcagacgacg gaaggattga tgaccgtgaa ccctgaagac gtgcagatcg
    10921 gtcagtttgt acttgtgaag cctggtgaac gtgtaccgtt agatggaacg atcatcgaag
    10981 gagaatcatt agtcgatacg tcagcactta caggagaatc agtccctaaa tctgtatatg
    11041 ttggtgaaac agtattaagc ggatttatca ataaaaataa accattttta gttcaagtag
    11101 aaaaatctta tgaaaattct actatcagca aattattaga attagtagaa aatgccagca
    11161 gtaaaaaagc accagctgaa aatttcatta ctaaattcgc gcgctattac acaccagtcg
    11221 ttgtaggatt ggccgtttta ttagctgttt tacctccact tgttgtttct ggagcggcgt
    11281 tttctgaatg gatctatcga gcattgacat ttttagtgat ctcatgtcca tgtgcattag
    11341 tgatttccgt gccacttagt ttctttggcg gtatcggagg cgcaagtaaa atcggtgtat
    11401 tagtaaaagg ttcaaattac ttggagttgc ttgcgcaaac cgagacagtg gtattcgata
    11461 aaaccggcac attaacgaaa ggtgactttt cgatccagac gatcgataca aaaatcgatc
    11521 caaagaaatt catgcaatat attgcgagtg cagaacagtt ttctacacat ccgattgctc
    11581 aatctgtttt agaaggctac gcaggtccgc ttttaccaac aactaatatc caggaatttg
    11641 caggtgaagg gatcttagct gaagttgatg gcaaacaagt attagcaggt aatcacaaat
    11701 tgatggaaag atttgaaatc tcttttcctt taagtcaaga aatcggtaca ttgctttatc
    11761 tagcgatcga tcaaagttac agtggttatc ttgttattgc agataccttg aaagaagatg
    11821 ccgtagacgc attggttcaa ttaaaacagg ctggtgtgaa gaacacagtc atgttgacgg
    11881 gcgactcgaa aaaaattgct gatcatatcg ggaagcaagt aggcgtggac aagatatata
    11941 gcgaattgct tcctgaagat aaagtgcagc ggttagagga aatcttacaa agaaacaata
    12001 aaaagaccgc atttgtcgga gatggaatca acgatgcacc ggtgctagca agagcagatg
    12061 taggaatcgc aatgggtgga ttgggaagcg atgctgcaat tgaagcagca gatgtagtca
    12121 tcatgaatga tcaaccatcg aagattgcag aagcaatcca tttagcaaag aaaacattga
    12181 aaatcgtcaa acaaaacatt gtctttgcta ttgggataaa aattttggta cttgccttag
    12241 gtgcttttgg ttttgcatca atgggagatg cagtatttgc agatgttggg gtgacagtat
    12301 tagctgtttt gaatgccatg cgaagcttgc atgtaaaaaa agaggacgaa tgaacgtcct
    12361 ctttttttta tatccttttc ttaatgaaaa atgtaaagaa aatagatagg attataaaat
    12421 aactaatgtt tttaattttg cagaaaattc aaaatagctt gaaattcaat aaggtcaatg
    12481 aaataaaaga aaataactta tttttttgat ggaaaaaata tgaaaaaacg atgtgtatta
    12541 aataaaagaa tacacacata aaataacact gtcttgtaag tttgttaaaa attgaatatg
    12601 attacatctg gataacaaat ttttatcata aaggagagaa cgaatgaaca acaaacaatt
    12661 acgctggttt actgttgcgt taattgcatt taacatggtt tggggcatgg ggaatgtagt
    12721 taataactat gctcagcaag gagtatcagt tgtcacctct tggctattga ttttggccat
    12781 ttattttatt ccatacgcat tgatcgttgg acaattagga tcagccttca aggatagtaa
    12841 aggaggcgtc agttcatggg tcgaaaatac gacatctaat aaacgactgg catactatgc
    12901 cgcttggact tactgggtcg tccatattcc ttatttggca caaaagcctc aagcaattct
    12961 gattgctggt ggttgggcag tccaaggaaa tggaaatatc gtaaatacga tgtcagtaca
    13021 aatggtagca gttatttcac ttattatatt tcttgccttt ttatatttat caacaaaagg
    13081 actttctact ttgaaagtga ttggcggact ggccggaaca gcgatgttcg tcatgtcgat
    13141 cttatttatt cttttagctg taacggcacc gttcatcaat ccaacgatgg aatttgccac
    13201 accacatatg aatcaagtga aaacatatat tccaacattt gatttcagtt atttcacaac
    13261 cgtttcgatg ctagtatttg cagttggagg ggcggaaaaa atatcccctt atgtcaactc
    13321 tacaagaaat cctgcaaagg aatttcctaa agggatgatc ttcttagcag caatggtagg
    13381 gatttgtgct gtattaggct cagtcgcaat gggtatgctg tttgctagtg ataatattcc
    13441 taaagacttg atggcaaatg gggcatacag tgcttttcaa attttaggta attactatgg
    13501 tatcggtaac tcattgatga tcatttacgc attgacaaac atgattggac aaatctccgc
    13561 attagccttt tcaattgatg caccactaaa aatcttgtta gcagatgcgg atccagaatt
    13621 tgtaccaaac tggctacgta aacgtaatcg taaaggtaca ttggtgaatg gttatcttct
    13681 gacaggtata ctagtaggaa tcattatttt gttgccgatt tttggtatca aagatatgaa
    13741 tgaattagta aaatggttga cgaacttgaa ctcagttgtc atgccaatgc gttacctatg
    13801 ggtgttcttg gcatatatga tgttgaataa agcttataag aaattcaatt ctgaatataa
    13861 atttgttaaa aatccaaaaa tcggttttat ctttggtgct tggtgctttg ccttcactgc
    13921 gtttgcatgt atcctaggaa tggtgccaaa agtagattat actgctgatc ctaaagcatg
    13981 gtggttccaa ttatttacga atatcatcac accaatcgtc ttgatcttat taggtatgat
    14041 cttgccagca attgctcgtc gagacaaaaa caaagaaatc actgaaccag aaacagtggt
    14101 tcagccttaa aatagaaata cttgttacaa gaggttgtga cagaagtggt cagcttcaag
    14161 aaataagaag gaatttacga aaattgcttc tcaaattttt gtgaagtttc ggcttgtttt
    14221 tcgaagaagc tacttctgga acaccgtgaa tcagaggttg tgaaaaagct gtcctgcatc
    14281 aagtaataag aacagccaaa caaaaatagc ccctcatatt tttcgtttgg ctgggcttat
    14341 taccgcagat gcaagctttg gaacaccgtt taaggaagag gttgtaaaat cagcgatttg
    14401 acctgagtat tagagaaaca aacggaaaaa tagctcctca tattttgccg ctttttgagc
    14461 taatgctgaa ggtcagctga ttgaacacca tttatt
//
LOCUS       DAFEOM010000066        13892 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.069, whole genome shotgun sequence.
ACCESSION   DAFEOM010000066
VERSION     DAFEOM010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.069"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(<1..692)
                     /locus_tag="LP524_002179"
     CDS             complement(<1..692)
                     /locus_tag="LP524_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL2235205.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKT"
     gene            965..3436
                     /locus_tag="LP524_002180"
     CDS             965..3436
                     /locus_tag="LP524_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBL2235206.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3539..5275)
                     /locus_tag="LP524_002181"
     CDS             complement(3539..5275)
                     /locus_tag="LP524_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBL2235207.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5272..5976)
                     /locus_tag="LP524_002182"
     CDS             complement(5272..5976)
                     /locus_tag="LP524_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2235208.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDLHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            6107..6610
                     /locus_tag="LP524_002183"
     CDS             6107..6610
                     /locus_tag="LP524_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBL2235209.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6570..6908
                     /locus_tag="LP524_002184"
     CDS             6570..6908
                     /locus_tag="LP524_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBL2235210.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6929..8347
                     /gene="ffh"
                     /locus_tag="LP524_002185"
     CDS             6929..8347
                     /gene="ffh"
                     /locus_tag="LP524_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBL2235211.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8459..9037
                     /locus_tag="LP524_002186"
     CDS             8459..9037
                     /locus_tag="LP524_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBL2235212.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            9041..9562
                     /locus_tag="LP524_002187"
     CDS             9041..9562
                     /locus_tag="LP524_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL2235213.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9641..10195)
                     /locus_tag="LP524_002188"
     CDS             complement(9641..10195)
                     /locus_tag="LP524_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBL2235214.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10448..10723
                     /gene="rpsP"
                     /locus_tag="LP524_002189"
     CDS             10448..10723
                     /gene="rpsP"
                     /locus_tag="LP524_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBL2235215.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10735..10986
                     /locus_tag="LP524_002190"
     CDS             10735..10986
                     /locus_tag="LP524_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBL2235216.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            11111..11902
                     /locus_tag="LP524_002191"
     CDS             11111..11902
                     /locus_tag="LP524_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235217.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            12072..12593
                     /gene="rimM"
                     /locus_tag="LP524_002192"
     CDS             12072..12593
                     /gene="rimM"
                     /locus_tag="LP524_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBL2235218.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12583..13344
                     /gene="trmD"
                     /locus_tag="LP524_002193"
     CDS             12583..13344
                     /gene="trmD"
                     /locus_tag="LP524_002193"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBL2235219.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13480..13827
                     /gene="rplS"
                     /locus_tag="LP524_002194"
     CDS             13480..13827
                     /gene="rplS"
                     /locus_tag="LP524_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBL2235220.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
BASE COUNT         4573 a         2554 c         2864 g         3901 t
ORIGIN      
        1 gttttaagca actgacaata gctggcattc atgtccatca ctaggtactt gacctttaaa
       61 cgggcctttc taggaaagcg caggaagtat tgagttagtt tgtaaagtct gcgatcttca
      121 aggatcgtaa atagctgatt ggttgtccca tcaacacaaa taaaactcat ggaaccagaa
      181 acagaattca ttgatttgaa ctcatccata cacaacaccg tcggaaggaa gttcatacgt
      241 ggctggtaat gcttcgttgc ttcacgcaag atccgtgtca ccgtcccatc cgagacgaag
      301 tacttcttag cgattagttt acgtgattga ttttcgtaca gttccatgag aatcacctgc
      361 ttcagttcct ttgttagttg atgatgttca tcaactaagt ccgttttcgc agaaaaggtc
      421 cttccacaat catggcagag aaaacgggta cgttttaatt ctaaaatagt taacttttct
      481 ttgactttca acatctgagt gcgtgtcgta taggaaccgt tgcggataat acgaaccgat
      541 cgacagtgtt cacaatagga agaagagtca tccaattttc ctttaataaa ctgcgcttga
      601 acgcctttat attctttttc aatcagccat tcttcatcaa aaaataaatg tttatctgtt
      661 aatccgagta attttctagt atgattatcc attgagagac tcctttggta ggatgacgtt
      721 ttggtcgact aaattttacc aaaagtgagt ctctttttga gttgaaacga agaaaaagtg
      781 ttagtagaaa atcgccttcg attttccacc aacacaaaat attatagaac cctttttttt
      841 tgctatatga caggaatgaa aggtgtttgc aagcttaatg tgaggaacac tttccatatc
      901 tgttctatac tggataggag aagtttggaa atatccagaa aacgaaatca atcgaggtag
      961 ccacatgaag aataaatgga taaatcgaag taaaaaaaag aaagtaacac aaagatcatc
     1021 agataaagtc atccgtcttt ttccatcgta taaagaagga ttaacggcag atcaagtcaa
     1081 ggaacgaata gaaaaaggtg cagcaaataa ttcagtcgat cccacattca aaacaaatca
     1141 gcaaatcgta ttggaaaaca tatttacgta tttcaatttg atctttctta tcttagccat
     1201 tttgctttgt ttagtagaat cctataagaa cttgactttt ttaccagtta tcatagccaa
     1261 tacaggaatc gccatttttc aggaaattcg atccaaaaag atcttggacg aactaaatgt
     1321 tttaaataca gcaaaagtca atgtcatacg agacgggata gaacaaacaa tagatataga
     1381 agaccttgta atagatgaca ttgttatttt agagactgga tatcaaattc cagcagatgc
     1441 agaggtcgtg gaagggaaac tgcaagttaa tgaggcttta ttgacaggcg aagcagatga
     1501 aataaccaaa gaaataggtg atccattaat gtccggcagt ttcattgttt ccggaaaagc
     1561 ttacgcccga ttagacaaag ttggagcaga ttcgtatatt tcccagctga cagtcaaagc
     1621 caagacaatg ggagaaggag aacagtcgga gatgattgct tctcttaatc aattgatcaa
     1681 atggattggg atcatcatta ttcctatcgg tatcctgctt ttttctcaaa gttacttttt
     1741 taatggaaac acgatcaaag aaagtgtcgt tgcaatggaa gcagcattga tcggtatgat
     1801 acctgaagga ctgtacttgc tgacgacgat tgcattagca ttaagtgcga ctaaactagc
     1861 aaagcaaaga gttcttttac acaatatgaa aagcattgag acattggcaa gggtaaatgt
     1921 cctttgtgta gataaaacag gaacgatcac agaaaataaa atgtctgttc aaaaaatgat
     1981 cgtctctaaa aaacaagaaa taatcagtca gtcagatcaa ttggaagcat gcattgctga
     2041 ctatgcaaaa gcgatggcag gagacaatgc aacaatggaa gctgttaaag catattttac
     2101 aaaaaccact gatcaatcct atcaccatgt tattcctttt tcttctgtcc aaaaattcag
     2161 cagtgtagcg ttggcagaca aggtgtatgt tttaggagcg cccgaaatgg ttttgcgcga
     2221 tcaattgccg gaatatgcgg aagagtttgt ttctttcgct gaaaaaggct atcgcgtgtt
     2281 ggtctttggt gtgtacgatg ggattcttga agaaccagta ttgacagaag cagtcacacc
     2341 attaggctat attctgattg ctaatcctat cagaaaagaa gcaagagcca catttgatta
     2401 cttcaaaaaa cagcaagtag ccatcaaagt gatttctgga gataatccgc tcaccgtatc
     2461 caatgtagct gtccaagcag ggatttcaca tgctgatcag tacgttgatg catctaaatt
     2521 atcagaagaa gaatatgaag cagctgtcga aaaatataca gtttttggac gcgtcaagcc
     2581 agaacaaaag aaaatcttcg ttcaattgat aaagcgtaac aatacagtag ctatgacagg
     2641 tgatggcgtg aatgatattc tagccatgaa agaagcagac tgcagtatag cgatggcttc
     2701 cggaaatgaa gccgccatgc aggcctcaca agtggtctta cttgattctg acttttcacg
     2761 catgccagaa gttgttgcag aaggacggcg cgttgtgaac aatattgagc gatcagccag
     2821 cttgttttta gtgaagaata ttttctcctt tttgctctca ctgttttcag tgatctttgc
     2881 tctgacctat cctttagaac catcacaaat cacattgatt agtttgttta ctatcggact
     2941 tccatctttc ttgcttgctc tagaagaaaa caaaaagcga atcagaggga aattcatcat
     3001 gaatgtgatg gaaaaagctg tgccaggtgg actaacagat atgatcgtag tcggtgcgtt
     3061 ggtgatctgc ggggtgactt tgaatctaaa taaaacagat gtttctacag catctaccat
     3121 gcttttgatt gcagtagggt tcctcgttct ctataaaatc tgttcaccgc tgaataaatt
     3181 cagaagtcag atcattcttt tctgtgctag cggtaacttt ttttcggtca tctttttgca
     3241 taagctgttt tcaatcaccg gtatctcagc tgtatcactt ttgctgttga gcattctatt
     3301 tttctcggca gattcgattt ttaggcattt gaccacattg gtagaatatt tgtttgaacg
     3361 taaaaatgaa aaagagcaaa agccgtttag tcgcgtagct aaaaaatttc tatcgatatt
     3421 taaacgagaa aattgacgaa aagtatctca aaaaagagta aaaaagaaca accactcgtg
     3481 tatctttcga agcaggatga gtcatccaac tttaaaacgc acgagcagtt gctctttttt
     3541 acattttcgg aatggtaacg gtgaaagtcg aacctgttcc aagatgactg tctacagtaa
     3601 tcgtaccatt aaggagttgc acgtagtctt ttacaatcgc taaccctaat ccggtcccgc
     3661 ctgaatgtcg gcttcgcgcc ttgtctacgc ggtaaaaacg ctcaaagatc cgttcctgat
     3721 cctcaatgtc gatacctata ccaaagtctt ggacagaaaa cgatagatca tctgttgcct
     3781 gatagcggat aataatctcg ctgtcgcttt ttgaatattg gacagcattt tcaattagat
     3841 ttttgataat tggataaagg atatctgttt tagtagtaaa tggttcgttc tttccaatca
     3901 atcgaatcgt caaccgtttc ttttcgatga ttggctgata attttgaacg atttgctgga
     3961 aataaggttc tgtataaatt gtctggatct cgtaagaatt ctcgccatct tttgacagct
     4021 ggatgatttc ccgaatcagt ttatctaacc ggatcgcatc tttttgcatg atctcaagaa
     4081 aagaagtcag agtttgcggg tcatcttttg caccatccag taaagtctcg gtaaaaccaa
     4141 tcagtgaagt cacaggagtt tttaattcgt gagagacgtt gccaacgaaa tccttttgta
     4201 gcttttctaa acgtcgcact ttcgttaagt catacgccac ccctagaatc tgtccggtac
     4261 cttctgaatt agtaaagtag cgcaaactca agtctaatgt ctgccgacct tctgtaaggg
     4321 tgatctcctg atggatcagc ggatgctcag gagtcacttg atgaatcagc tgaatgagtt
     4381 ttggctcttg aatgacttct gtgtaccgct gttcaggttg atagcttacc acgtttaaat
     4441 gtagctgcat ctttggatta agcagtagta aacgagaatc agcatcatca atcaggaata
     4501 cgccaatcat cagttcgttc agtaacgtat ataattgctc ttctgtggta gtacacgccc
     4561 gatacatctt actcatttgt tcgctcaacg cattaatcgt ctgatacaat tcttcccatt
     4621 gatcgggtgt ttgcatgatt acttccgcct gattaggatt ttgcaccatt ttttttagta
     4681 cgggcagcac ggttttcaat ggctggtttt tttgatagat cagataatag atcattcctg
     4741 caagaacaag gaagaaagac agaaagaaga aaaagaccca acggcgaaaa ctctcagttc
     4801 tcggtaaaaa accgcttgtc ggttctgcga tacgtaaaat ggcttctaac tggccacttt
     4861 ttttgactgg taatgcgaca tataaaagat cctcattcaa agtagtactt ttgcgcaaag
     4921 cgctacctag gttcccacca ttcaatacgg ctttgatttc gggacgggag tttcgctgtt
     4981 cattcaatgt ttcattgttt gtgtcaaata agatctttcc agtagcatcc attaaagtaa
     5041 tccgttcgtt cgactgatgg acgaattctt ccaaagcagt tttgttttgc gctgcttgga
     5101 ggttttcgac atctaattgc cggatcagca gttgtgcttt ttttgtcagg tagctttctt
     5161 gctgttccac gatctgctgt tgaaaataat gggaaatcaa ttgccagcca ccaaaaaaca
     5221 aactaactaa aagaataaga aagaagagac gttcataaat ttttttccat ttcatcgttt
     5281 gggttcctga aacttatagc caaatcctct aatagtcacc aagtactttg ggtgttttgg
     5341 atctcgctcg attttttctc gcaaatgact aacgtgtaaa tctacgattc ggctttgtcc
     5401 tgcgaagtca aaattccaaa tgcggtctag caatgtgtct cggtcgatca cacggtcttt
     5461 tcgtttcatg aaataaacaa gtaattcaaa ttctttcggt gttagttcaa tcagatggtc
     5521 gtctacggtc acttgataat tggtcaaatc agcagtgatt tgtccaatcg atagatattc
     5581 aggctcagct tcatcttttg attgcttacg aggttcaatt cgtctgaata ttgctttcat
     5641 acgggctaat acttcgcgag gactaaatgg tttggtcaaa tagtcgtcag cacctatttc
     5701 taggccaatg atccgatcca cttggtcatc tttagccgtc agcatcaaaa tcggtgtttc
     5761 atttttttct tgacgcagcc gctgagtgat ctccatgcca tccatcgttg gaagcatcac
     5821 atctaaaatg atgaaatcaa atgattgttc taatgctaat tctaatcctt cgcctccatc
     5881 tgtggcactg gttaccttgt agccttcttt ttccaaattg aacgtcaaga gcgttacaat
     5941 agaaggttca tcatcaacaa ctagtatttt tttcattcaa atctccttta aataggtttc
     6001 attctgttta tattttagca tagaatcagt acaaagggct attttacaaa agaaataaat
     6061 ggtataatag gaaggcactt tgctgaatag aaaggaagag cagtcaatga ttggaatcag
     6121 tgcatgttta ggcggtctgg cctgtcgcta tgatggacag aaaaaagaag taccagaatt
     6181 gatggaacta gttgaaactg gagaagctgt catggtctgt ccagaagtga tcggcggatt
     6241 accaattcct agagatcccg ctgaaatcat tggtggaaat ggattcgatg tgtggaaaga
     6301 tcaagcaaaa gtatggacga caagcggcga agatgtaacg gaagcgtaca aaaatggcgc
     6361 gatcaaagcc tatcaaaaat tgcaggaaaa acacattgat ctagtcatca tgaaggaaaa
     6421 aagcccttca tgcggaaccc attcgattta tgacggaagt ttttccggaa taaaaaaaaa
     6481 tggtgccgga gtggctactg cttattttat tcagcaaaaa atgactgtgc tctctgatga
     6541 agaatggctg aaaactagag gtgagcagaa tggaaatcga aaagaccaat cgaatgaacg
     6601 cgctctttga attttattcg accttattga cagaaaaaca aatgaactac atggaattat
     6661 attatgcaga tgatttttca ttaggtgaga tcgcggaaga atacgaagta agccgacaag
     6721 cagtatatga caatatcaag cggacaagca agatcttgga ggattatgaa aaaaaactcc
     6781 atcttttttc taattatatt gttcgagaac aggtattgga aaatatgact gcatatatta
     6841 cagataagta cccggaagat aaaaaactac ttgaatatgt acagcaaatt caagcaatcg
     6901 aagaataaat aaaaggaatg aagaaaatat ggcatttgaa agcttaactg aacgtctcca
     6961 gcaggcgatg ggaaaaataa gaaaaaaagg aaaagtatca gaagctgacg taaaagaaat
     7021 gatgcgggaa atccgccttg ccttactaga agccgatgtt aacttgcaag tagtcaaaga
     7081 ttttactaag cgcgtacgcg aacgtgcggt aggggcagaa gtgctggaaa gtttatcacc
     7141 tgcacaacaa atcgtaaaaa tcgttgatga agaattgacg atcacacttg gttctgaaac
     7201 agcagagtta aataaatcac caaaaatacc aactgttatc atgatggctg gtcttcaagg
     7261 ggcaggtaaa acgaccttta ctggtaaatt agccaattat ttaaagaaaa atgaaaatgc
     7321 tcgtccgctc ctaatcgcag gggacgtata tcgaccagct gcgatcgacc aattgaaagt
     7381 attaggtcaa cagctagacg ttcctgtatt tgatatggga actgaagtca gcccggtaga
     7441 gatcgtccgt caagggatgg agttagcaaa agaaaagaaa aacgactatg tattgatcga
     7501 tacggcagga cgtctgcata tcgatgaaac attgatggat gagttgaaac aaatcaaaga
     7561 attgacgcaa ccaaatgaga ttttgcttgt tgtcgatgcc atgactggtc aagatgccgt
     7621 caacgtagct gatagcttca accagcaatt aggtattaca ggggtcgtca tcactaaact
     7681 tgatggggat actcgaggcg gggcagcact atcgatccgc tcagtgacag gcgcaccaat
     7741 caaattcatc ggttctggtg aaaaattgac tgacttagaa gtattccacc cagatcgtat
     7801 ggctagccgt atccttggta tgggggatat gctgacattg attgaaaaag cgcagcaaga
     7861 ctacgacgag aaaaaagcgg aagaacttgc acagaaaatg cgggaaaatt cattcgattt
     7921 caatgacttt attgaacagc ttgatcaagt gatgggtatg ggcccactgg aagatctaat
     7981 caaaatgatc ccaggaatga atcaagtccc tggaatcgaa aacgtcaagg tagatccaaa
     8041 agatgttgaa cgcaaaaaag cgatggttta ttctatgacg ccagcagaaa gagcaaatcc
     8101 tgatctgttg aacccaagcc gtcgtcgccg aattgctgcc ggttctggaa acagtgttgt
     8161 tgaagtgaat cgaatgatca aacaattcaa ggaatcaaga aaaatgatgc agcaaatgtc
     8221 caaaggggat atgaacatcc caggtatgga ccaaatgttt ggttcaggcg tcaaaggaaa
     8281 actaggcaaa atggcgatga atcgaatgat caagaaaaat aagaagaaaa agaaaaaacg
     8341 gaaataagat gaaaaaaaca gcttcggtac agactgaaaa tgtctactgc cggagctttt
     8401 ctatcagcaa tttttattct ttactaatac aattactcta aacgaagagg tggcacagat
     8461 gagatgtccg tggagtgaac aaacagaaag tatgcggcaa tatcatgata ctgtctgggg
     8521 tgttcctgaa tatgatgacc aaaagctgtt ccgaaaattg atgcttgata ttaatcaagc
     8581 cggattgagc tggcagacga ttttaaataa aagtgaagca ttcgatgaag catatgatcg
     8641 attcgatatc aaaaaagtcg caaattatgg agaaacaaaa atcagtgaac taatggaaaa
     8701 caaaggaatt attcgtaacc gaagaaaaat cgaagcagcc gtccaaaacg ccaaagcagt
     8761 cttgaaaatt caagaagaaa tcggcagttt ttcagagtat ctttggtcgt tttctaatgg
     8821 aaaaatcgtt gattcttctt atcattcaca agaagaagtc ccaacaacaa gtcctctttc
     8881 agatgaatta gcaaaagatc taaagaaaag aggatttaaa tttatcggaa gcacaacaat
     8941 ctatgctttt ttagaagctg tcgggatcat caatgatcac cttgacagtt gtttccgcaa
     9001 agaagaggtc gtacgtatcg gcagaaaggg cgaataaagc atgcgaaaaa tcatatttta
     9061 tggtgctata agtttagatg gctatttggc aacgaaaacg gatgatcttc aatggctttt
     9121 tgacactcca acaggtaagc aaactactta tgaagctttt tatgaaacaa tcgatacgac
     9181 catcatgggc agaaaaactt atcaggaagc aaaaaaatat ttggagaccg aagcaattta
     9241 tccagagaaa aagaatttcg ttttttccac ccacgcacaa ttacaattgc cagatgctga
     9301 ggtcgtaaac gaagatcctg ttgcttttct aaaaaatcta caaatgacag aaggaaaaga
     9361 tatctgggtt gtgggtggcg gtcgattgat caaaccgcta ttggaacatc aaatgattga
     9421 tgaatggtat atccagatta cccctgtatt attaggggac gggattcctt tattccaaaa
     9481 aggaacatat gaagcgcgtt ttgaaatgct agacacgaca cgattcggtg aatttatcga
     9541 attgcattat gtacgaaagt agaagtaaat aaaataaaaa aggcaactat atgcagaaat
     9601 gtcctgtaca tagttgcctt ttttattacc gttatgcttt ttactctgtt aatttgttag
     9661 aaaaatctag atacgcatct aaaatcgttt ggaagaaact taatgttcct tcaacgatat
     9721 taccattttc atcaattaga ttcgcaacgt ttccgatata tgcttccggt tgctgcaagg
     9781 ttggcacatt caaaaagaca agtgattggc gtagatggtg gtttgcgcca aagccgccga
     9841 tcccacctgg tgatgcagta acgactaaac ctggtttatg atcccaaaca ctgtgtccat
     9901 aaggtcttga gcctacatct aaagcatttt tcaatactgc tggcacagaa cggttgtatt
     9961 caggagtaac gaaaacgaca ccgtctagtc ccctcacttc ctcacgaaaa cgagtccatt
    10021 cagcaggagc ctgttctgga gtttcgagat cctcattgta aaatggcaga tcatcgattt
    10081 ttacaaatac actttcgtac ccttcaggaa gcaatttcgc aaaagcttct gctacttttt
    10141 tgttgtaaga atcttttctt aaactaccaa taaaaaatcc aatttttttt gtcatacgat
    10201 ttttcctcct tcaaactatc tataataatc ttacaaaaga taagtaactg gagcaaataa
    10261 aaacacttac tgacaaaagc tacatagaag atagattact tatttttaac aaaaaagaag
    10321 tctaaaatct tctattttct aaaaaaaatc gaatttaatc aagctgtaaa gaaaaaaagc
    10381 tttacagggt aaaaaaaaac tgttacacta catcttgtga ataaaacaat ggaggtggaa
    10441 ataaataatg gcagttaaaa tccgtttaaa acgtatgggt tctaaaaaga gtccgtttta
    10501 ccgtatcgtc gtagctgatt cacgttctcc tcgtgatgga cgtttcatcg aaactgtagg
    10561 tacttacaat cctttgaaag accctgcaga agtagtttta aaagaagact tagttcttga
    10621 ctggttatca aaaggtgcac aaccttctga cactgtacgt aacatccttt caaaagaagg
    10681 cgttatgcaa aaacaccacg aagctaaatt ctcaaagaaa taaggtgacc ggatatgaaa
    10741 gatttaagcg acttagtctt aacaatcgtt cgtccgctag tcacttatcc tgatcaagtc
    10801 aagttagacg tcgtagagtc gaaagacttt tacgagtaca atctaacagt ggctcctgaa
    10861 gatattggcc gtatcattgg aaaacaaggg cgtgttgcta aagcaatccg cacaatcgtt
    10921 tacggtgttc ggatcaatgc accgaaaaaa gtgcgtttga atatcatcga tgataaggaa
    10981 aagtaaataa acgtgctgta ctcgaatttt cgagtgcagc ttttttgttt tttttattaa
    11041 ttgaagaaat gattatttgg caaaacaagc aagaaagcgt atccttatag gaggagggag
    11101 tgaacagcca atgagaaaga aaatgttatt tattccattt gctctattgt taagcgcttg
    11161 tagttcggtt actcaagaat cagacaatac acatctatca agcagtatat ctgaaactgt
    11221 taccagtcca gaaaaaagta gtaccaaaac ggcttccacc actgaacaaa caatggctac
    11281 ctcaaataat gagaaaaaga ctgctttaga ccagttgaaa gaacaacagc caaatgttcc
    11341 catgcctcta gatgtaccag tctcttcagg ctatctcaat atcgcagcga cacatacaaa
    11401 gcaaggttat tctattttat attacagaac agatcgtccc ctcgggttga atgctgacga
    11461 attgaatcaa gaaacgccta ttgcaaccta tctatatcaa tatgggtttg cttctagtca
    11521 agaaacaatc caagttttgc agccatttga gatcgacaca aatggtcagc aagtggactt
    11581 aggttctcga atcacagggt atcagcaagg tgcagcaggt tcaagttttc ttgaatggca
    11641 agaaggaaac tggcgtattc gaatccgtgg gaataatata gaaggccaag atcccttact
    11701 attagctaaa gaaatcgtag cttatttgga agaaaacagt ctaccagcac ctgaacaatt
    11761 tggtaaaatt actgttgata tgggtgatac gacgaatcga gccgtagaag taagctggca
    11821 agaaccgaaa aatgcctata cgatcactca tcaagatcct atgtctgcgt tgaagatggc
    11881 agtttctatg aagaggttgt gaaatcagcg cgctgatctg agcccatcat ttaactccaa
    11941 gtcacaggaa gtaatcaaaa atagcacgcc gcatttttgc ataattttgg cttaatacct
    12001 taaaagaaaa ctagaattgc ctcttcacac atgctaaaat aaaacagaca aaaaagaaat
    12061 ggggatataa gttgactgaa tacttgaatg taggaaaaat cgtaaacaca caaggtatca
    12121 aaggcgaagt ccgtgttatc tcaacaacgg atttcccaga agaacgttac aaaaaaggaa
    12181 cggtgctaac actattccaa gacggaaaag cacctgtgga attaacagtc aaaagtcatc
    12241 gcaaacataa aaattttgac ttattgagtt ttgaaggaca tccttctatc aatgatgtgg
    12301 aaaaatatcg ggatggcata ttaaaagtgt caaaagacaa ctctgtagat ctagctgaaa
    12361 atgaattcta ttaccatgag atcatcggtc tgcatgtatt agaagatgga actgagttag
    12421 gtaaagtaaa agaaatcttg tcaccaggtg ccaatgatgt ttgggtcgtt caacgtccga
    12481 aaaaagctga tatattgctt ccatatatcg cttctgtcgt caaagaagtc gatttagaag
    12541 ctggagtggt ccgtgtagaa attccagaag gactgattga cgatgaagat tgatgtattg
    12601 actttatttc caagaatgtt tgaaggtcct atgggggaat ccatcatcgg taaggctgta
    12661 gataaaggat tattagatat caatatctct aacttccgcg attattcaga taacaaacat
    12721 cagacagtag atgattatcc atacggcggt ggagcaggta tgctgctcaa agtccagccc
    12781 atctatgata acatccaagc cattgaaaaa gctgcaccgg atgtcaaaaa aagagtaatt
    12841 cttcttgacc cagctggtaa acgattcgat cagaaaatgg cagaagaatt ttcacaagaa
    12901 gaacacttag tattcatttg tggtcattat gagggatatg atgaacgtat ccgcacatta
    12961 gtgacagatg aagtttcctt aggagattat gtccttactg ggggagaatt aggtgcgatg
    13021 gtcatgatcg atgcaacagt tcggcttttg ccagatgtgt tagggaatca gacttcagct
    13081 caaacggatt cttattcaac cggtttattg gaacatcccc aatatactcg cccggcagaa
    13141 tataaaggga tgaaagtacc agaagtattg acaaatggaa atcataaatt gattgaagaa
    13201 tggcaattaa aagaatcact aagaaggacc tatcaacgtc ggcctgatct tttggaaaca
    13261 cttgaatgga cacctcaaat gacgaaattt ttagaagaaa tcaagaaaga agaacaagaa
    13321 aaaacagcag atgaagccaa gtgacttttt ctaaaatctt ctttacagta ggttgataat
    13381 atgttagtat tttatggtga gtgcaaaagc acttaactat tacgatattc cgctgtgaga
    13441 aaagctcata agaatatttg gaataaggag aagaaaacca tgaatccatt aatcgaagaa
    13501 ttaacaaaag aacaattacg ttctgacatc ccagcgttcc gccctggtga cactgtgcgt
    13561 gttcacgcaa aagttgttga aggtactcgt gaacgtatcc agttatttga aggtgttgtt
    13621 atcaaacgcc gcggtgctgg aatcagcgaa acttatacag tacgtaaagt ttctaacgga
    13681 gtgggcgttg aacgtacatt cccattgcat acaccacgtg ttgcaaaaat cgaagtagtt
    13741 cgctacggta aagtacgtcg tgcaaaattg tactacctac gtgcattaca cggaaaagct
    13801 gctcgtatca aagaaatcag aagataaaca cggaaaactt ccttggaaat ctgaaatttg
    13861 tgaaaagtcc ttgtatatca agggcttttt ct
//
LOCUS       DAFEOM010000067        13868 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.070, whole genome shotgun sequence.
ACCESSION   DAFEOM010000067
VERSION     DAFEOM010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.070"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="LP524_002195"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="LP524_002195"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBL2235221.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="LP524_002196"
     CDS             complement(2260..2511)
                     /locus_tag="LP524_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HBL2235222.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="LP524_002197"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="LP524_002197"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBL2235223.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="LP524_002198"
     CDS             3461..3679
                     /locus_tag="LP524_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235224.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="LP524_002199"
     CDS             complement(3968..4480)
                     /locus_tag="LP524_002199"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBL2235225.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="LP524_002200"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="LP524_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBL2235226.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7376)
                     /locus_tag="LP524_002201"
     CDS             complement(6933..7376)
                     /locus_tag="LP524_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HBL2235227.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="LP524_002202"
     CDS             complement(7377..8165)
                     /locus_tag="LP524_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL2235228.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /locus_tag="LP524_002203"
     CDS             complement(8162..9103)
                     /locus_tag="LP524_002203"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBL2235229.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="LP524_002204"
     CDS             complement(9280..9867)
                     /locus_tag="LP524_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HBL2235230.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11395)
                     /gene="obgE"
                     /locus_tag="LP524_002205"
     CDS             complement(10088..11395)
                     /gene="obgE"
                     /locus_tag="LP524_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBL2235231.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11689..12699
                     /gene="gap"
                     /locus_tag="LP524_002206"
     CDS             11689..12699
                     /gene="gap"
                     /locus_tag="LP524_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBL2235232.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12784..13719)
                     /locus_tag="LP524_002207"
     CDS             complement(12784..13719)
                     /locus_tag="LP524_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL2235233.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3835 a         3102 c         2445 g         4486 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa
    11701 gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc
    11761 tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa
    11821 ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac
    11881 tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc
    11941 ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct
    12001 gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca
    12061 gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac
    12121 aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta
    12181 aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca
    12241 caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct
    12301 gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag
    12361 ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact
    12421 gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa
    12481 aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat
    12541 gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct
    12601 ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc
    12661 caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa
    12721 agaagccttt gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact
    12781 cgttcaatga acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc
    12841 tgtcttgtat gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt
    12901 cgttgcacga tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg
    12961 tataaagagc attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa
    13021 aaagtagcca gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg
    13081 aagactaaac atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca
    13141 atcttcaatg attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt
    13201 tgctgctccc atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag
    13261 cactttgttt atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc
    13321 cccttcgctt tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg
    13381 aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag
    13441 agctgttttt tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc
    13501 ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc
    13561 cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga
    13621 aggcacaaca gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc
    13681 taaagcgacg cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc
    13741 ttttttcata taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt
    13801 ttataacccg cgaattgtaa aattaagtta gaaaaataaa aaggcattta tggtacactc
    13861 aaattgta
//
LOCUS       DAFEOM010000068        13427 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.071, whole genome shotgun sequence.
ACCESSION   DAFEOM010000068
VERSION     DAFEOM010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.071"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(<1..912)
                     /locus_tag="LP524_002208"
     CDS             complement(<1..912)
                     /locus_tag="LP524_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBL2235234.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1340..1903
                     /gene="efp"
                     /locus_tag="LP524_002209"
     CDS             1340..1903
                     /gene="efp"
                     /locus_tag="LP524_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBL2235235.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2004..2174
                     /locus_tag="LP524_002210"
     CDS             2004..2174
                     /locus_tag="LP524_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235236.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            2389..3648
                     /gene="lysA"
                     /locus_tag="LP524_002211"
     CDS             2389..3648
                     /gene="lysA"
                     /locus_tag="LP524_002211"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBL2235237.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3635..4435
                     /gene="dapF"
                     /locus_tag="LP524_002212"
     CDS             3635..4435
                     /gene="dapF"
                     /locus_tag="LP524_002212"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBL2235238.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4438..5319
                     /locus_tag="LP524_002213"
     CDS             4438..5319
                     /locus_tag="LP524_002213"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBL2235239.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5316..6032
                     /locus_tag="LP524_002214"
     CDS             5316..6032
                     /locus_tag="LP524_002214"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBL2235240.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6038..6733
                     /gene="dapD"
                     /locus_tag="LP524_002215"
     CDS             6038..6733
                     /gene="dapD"
                     /locus_tag="LP524_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBL2235241.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6749..7750
                     /locus_tag="LP524_002216"
     CDS             6749..7750
                     /locus_tag="LP524_002216"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBL2235242.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEKPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7747..9108
                     /locus_tag="LP524_002217"
     CDS             7747..9108
                     /locus_tag="LP524_002217"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBL2235243.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9068..10219
                     /locus_tag="LP524_002218"
     CDS             9068..10219
                     /locus_tag="LP524_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBL2235244.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10194..11363
                     /locus_tag="LP524_002219"
     CDS             10194..11363
                     /locus_tag="LP524_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBL2235245.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11538..12917
                     /locus_tag="LP524_002220"
     CDS             11538..12917
                     /locus_tag="LP524_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL2235246.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13198..13427
                     /locus_tag="LP524_002221"
                     /pseudo
     CDS             13198..13427
                     /locus_tag="LP524_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4483 a         2339 c         2923 g         3682 t
ORIGIN      
        1 gtaaatgttt tttgataaat agcggtctgc tgcgtctgga aaaatcgtaa cgacacgatg
       61 accaggtggt aaacgacgaa tttctcttaa tgctgctgca aaagccgcac cactcgaact
      121 tccaactaat agtccttgtt ctcgtgctaa ctgcctagta tagttaaacc cttctacatc
      181 agagattgtc tcgatctgat tgattgaaag gggagaaaga aacggcggaa tgaattctac
      241 accgataccc tcaatttcgt gaggggctgg atcaccacca tttaacacag agccctctgg
      301 ttctacaccg attattcgaa tgtcgggata tttttctttc aaaaaggtag aagttccagc
      361 aaaggttcca ccactcccaa ttcctgctac aaaactatgg atattttctt tgatttcatg
      421 aaagatttct ggccctaacg tatgatagta agcagcagga ttatctttat tttcaaattg
      481 aagaggcaga tagctgtttg atatctcctc tgcaagtttt ttacttttgc tgatggcacc
      541 gatgattcct tgctcactag gagaatggat gatctttgct cccaatgctt tcatcaattg
      601 ttgtttttct aagctaaact tctctggaac aacaaaaacc gttcttaatt tgtatgtgag
      661 tgcggccaga gcgatcccaa ttcctgtatt tccggcagtt ggttcgatga tcgtcgtttt
      721 ttcattgatt tttccttggg caaatccttc tttgatcaaa taactgccca atctatcttt
      781 gatacttcct ccaggattta aatattctag ttttgcataa atagcagaac ctttaggcat
      841 agaatagtgc tgattgtcaa agcgataaag cggtgtggaa ccaatagctt ctgtaattgt
      901 cgagataatc atgtgggcct cctaaaaaaa gataataaaa aaaggagctg actattaaat
      961 cagctccttg aaagacatag agaaaaattg agtagataaa aagataagtt acctggtact
     1021 cattctatga agggcaatta atagacatga caaatagtat gctgctttta tgcaccatac
     1081 aacagcaacg attcaattgt ttttggttgc tgactgtcat gtgtatccct ccaagtaaaa
     1141 tttttattaa cgatagcatg aaaaaaaata aaagtcaaag gcttgaaaga aaaaaatatt
     1201 tcgatagcat cgtttacccg tgtttatttt tctgacaaat aggcatgaga ccttaaaaga
     1261 tagagaatac tctagttttc tggccggttg tctgatacaa taaagttgat tatgataaag
     1321 aaaagaagga gcaaaaataa tgatctcagc aagtgattta agagccggta tgactttcgt
     1381 acaagacggc aaactgatta aagttctgga tgcaagccac cataaacctg gtaaaggaaa
     1441 tacagttatg cgtatgaaac tgaaagatgt ccgttcaggt gcaacatacg acacaacgtt
     1501 cagaccagaa gaaaaatttg aaaaagcaca tatcgacaca aaacctgtac aatatttgta
     1561 cacgatggat gatacagctt tcttcatgga tttggaaaca tatgagcaat atgaaattcc
     1621 tgtagaaaca gtagaagaag aaatgaaata tatcctagaa aacatggaag taaaaatcca
     1681 atttttcggt agcgaagtca tcggcgttca attaccaaca actgtcgtat tacgtgttgt
     1741 ggaaacacaa ccttccatca aaggtgcaac agtcactggt tcaggtaagc cagcgacaat
     1801 ggaaactggc ttagtagtca atgttcctga cttcatcgaa acagatgaat tgttggaaat
     1861 caatacagca gaaggttctt atgtaaaacg tgcgtcaaaa taattcgcta acggattgat
     1921 aagaatttga aaacagagct tgacctcttg aaaggcagat catcggatct gcctttcaag
     1981 aggttttttg tggaatcagc aaattgaaaa ataaaaaagt atttgatttt atttatattt
     2041 tgaaagatga atggcaaaag ctagagaaag aaaaaactgc acttatttat gaagaaattg
     2101 aaatgattca taaaattgaa aatatccgaa ctattccgaa ctatattgaa gcgaaaatat
     2161 tttttcatca ctaaatagtg ttgaatttat gtttttatgt ataaatagct tctaatagtt
     2221 cgggtatttt gtttttataa ccatttttat tcttattttt aaaaaaatat ttcgaaatat
     2281 actagacaat tcagaaaatt ttgtttaata ttattccaat aaaattcaaa tctttatcaa
     2341 atgaaattcc aataaaaacg gaaaaaaagt gaaaagagga atgaaaaaat gacagaattg
     2401 ataattggcg gagtcacagc aagcacattg gcagaaaagt ataagacacc tctgtatgtg
     2461 tatgatgaag aaaagatgag acaaacgatg ctgtcattca agcgaggctt tgtatcgaaa
     2521 cgttttgata caaaagtgct ttacgcctct aaagcattcc aaacgattga gatgctgcat
     2581 ttagttcgtg aatatggatt cgggctagat gttgtcagtg gtggggaaat ctataccgct
     2641 ttgcaagctg attttcctag agatactatc tacttccatg gtaacaacaa gacgccagag
     2701 gaactgatct atgcacttga aaatgatttg ttgcatattg ttgccgataa tttgatggaa
     2761 gttgaattat tagcagaatt aagccaacgc tatttaaaaa aaatctgtat catgcttcgg
     2821 ttgaatgtcg gagtagaagc acatactcat gaatatatcg tcactgcgca tattgattca
     2881 aaattcggca tgagctatga aagtaaagag tgccaacaag cccttcagtt gataaaagaa
     2941 agcagctatc tagagttaga aggatttcat gctcatatcg ggtcacaaat ttttgatgtt
     3001 actgcttggt tagctgaaat cgacaaattg acgaattact taaccgattt tgaccaacct
     3061 ctttccttga atcttggcgg agggtttggg attcgttata cacaaaatga tcagccgatc
     3121 ccagtcgaag aggcactaaa aaaactgatt gaatatacgg aagaagcttt ggaaaaacga
     3181 aaactgacca ttcgtcaatt actgatcgaa ccgggtagaa gtattgttgg agaagctggt
     3241 acaacactat acacgattgg tttcatcaaa aagacaccac ataaaaaata ttactttgta
     3301 gatggcggga tgacagataa tatccgtcca gctctgtatc aggcagaata cgattgtgat
     3361 ttagcagcaa aactgtcgga agaaaaaaca gagaaagtga cgattgcagg aaaaatgtgt
     3421 gaatcaggcg atgtggtaat taaagaagct tatttgccga aagcagaatc gggtgatctt
     3481 ttagctgttt attccacggg agcttatgga tactcgatga gcagcaacta caatcgagcg
     3541 acacgaccag cagttgtttt tgtcaataat ggccaatcaa gactgattgt tcgcagacaa
     3601 gcattttctg atctactaag gggggaagtt tcctatgatc cttcataaat ataatggctg
     3661 cggaaatgat tttatcttat tagattacgc agaagacaca gattatagtc aattagccgc
     3721 ttctttatgc acaaaagaaa aatatgatat agacggattg atcgccgtaa aaatcgatcc
     3781 gttggaaatg atctattaca acaaagatgg cagccgcgcg ccgatgtgcg gaaatggcat
     3841 tcgctgtttt gctcgatacg taaatgatca gaacatgatg aatgaacagg aattcaacgt
     3901 agagacatta gcaggcacaa gaaaaattac ggtgttggat ccctcacctt tttattgcag
     3961 cgtagatatg ggagtgccag atgattctcc ggaaaaaatc aaagctaatc aaacttatcc
     4021 aataaaaaac caggaaatag agatcaatgg tgaaaaagta acgatcacta gtttattttt
     4081 aggaacgatt cataccgttg tttttgttga aaatgcgcgt gaggaagtct ggagaaaaag
     4141 aggtgaactg atctgtcatc atcctttgtt caaagaaaaa accaatgtca actttgtaga
     4201 agtagtcaat gaacaagaat tgatcgtccg tacctatgaa agaggagtag gttggacact
     4261 tgcttgcggt acaggttgct gtgcctctta tgtggttgct aaggaaactg ggaaaatttc
     4321 agcagatcag gtagttgttc acttggaaca aggagacttg atgatctctg gtgaggatgc
     4381 gatacaaatg gctggcccag cagttcatga atggacaaga atattggagg aatgaagatg
     4441 ataaaaggtt cgatcgtggc gctgatcacg ccgatgaatg aagagggatc agtagattat
     4501 gctggtttgg aaaaattgat ccaattccat ttagatgaac aaacagatgg attattagtt
     4561 ttaggtacaa caggagaatc ttctacgctc acacaatctg aggaggagca gatccttcaa
     4621 ctgacagtaa agaaagtggc tggccgtgtg ccggtgattg caggagctgg aacgaataat
     4681 acgaaagaaa cgattgaaaa agcgaagcat tttgcttctt tgggggcaga cgcacttttg
     4741 gtcatcaccc cttactacaa caaaacaagt gatgcaggac tagctgctca ttttacagca
     4801 attgcagaag cctcaccgat tccgcttatt ttgtataatg ttccttctag aacaggcatg
     4861 tcgattccaa tccatgtatt agtaaattta gcagaacatc ctaatatcat tggtttgaaa
     4921 gaagcctcag gagatatggc ttatgtgatg gatgccgctc ggttgattgg agaagaattc
     4981 ttcctttaca gtggaaatga tgatttgatc ctaccagtaa tgagtgtagg aggaagcggt
     5041 gtaataagtg tatgggcgaa tatccaacct aaaatagtcc atgaattagt aaaagatacg
     5101 caggacggaa gatggcagca agcaaaagaa aagcaattaa atgcacttga actgatccat
     5161 gccttgttca gtgagaccaa tcctattcct gtcaaagcag ccatgtcatt attagactta
     5221 ccttccggac ctctacgcct gcctttagtt tctctttcaa aagagaaaaa gaaacaatta
     5281 gctcagcttt tgttaaaaga aaggacggtt gaaaaatgaa aatcggactg attggcagtg
     5341 gacagatggg aaatagagtc gaagaagttg cacaagaaag aggagatacg gtccttcttt
     5401 tacggacggc acctaaagaa gcaactacgc ttgcttttac ggaactgcca gaactgctta
     5461 ttgatttttc tcatccagat aatttagaga aaatcctttc gtatagctta tcatatcaac
     5521 ttccgttagt cattggcacg acaggttata cacaatcaca gttccaagaa ataaaagaac
     5581 atgccaaaca tgtacctgtg atgtatagtg ctaatttttc gttcggcatc atggtgatga
     5641 atcaactaat caagcaagca gctgcaatgt tgcaaggctg gcagatcgaa ctgattgaaa
     5701 aacatcatag tcgtaaaaaa gatgcaccat ctggtactgc aaatatgctg cttcaaacaa
     5761 tccaagaagt gcaaaagctg cagcccgtat acaactggca agggaaaaaa agagaagaaa
     5821 accaaattgg tgtacacagt attcgtgcag gttctttacc tggtgaacat gatgtattgt
     5881 ttgctgctac tgatgaaata tttacgatca aacatgaatc tttttctaat cgaatctttg
     5941 ctgcaggcgc agtagaagca gcggactggt tgaaagatca gtcaccaggt tattacaaat
     6001 tagaagattt attaatggac aaaggggtgt aaaaaacgtg gaagctcaag aaatcatccg
     6061 ctacattcaa acggcatcta aaaagacgcc tgtgaaagtc tatctgaatg taaccgagcc
     6121 aatcgaattt aaagaaagta aagtttttgg tgaaggaagc agcttcgttg tttttggtga
     6181 ctatgagacg attgctccgg ttttagaagc tgaatcagaa aaaatcattg aaatcgaaat
     6241 cgaaactgct gcaagataca gcgcagttcc tttgctagat attaaaaaaa ttaatgctcg
     6301 tatcgaacca ggtgctatta tcagagatca agtgtcaatc ggcaacaatg ccgtgatcat
     6361 gatgggagcg attatcaata ttggtgcagt aatcggtgaa aacacgatga ttgatatggg
     6421 tgctgtctta ggtggacgag cgacagtggg caaaaactgc catattggtg caggagctgt
     6481 cttagcaggt gtgatcgaac cagcatcggc caaaccggtt atcgtagaag acggcgtact
     6541 ggtaggtgcg aatgcagtga ttgttgaagg tgtccatatc ggaaaagatg cagtcgtggc
     6601 agcaggagca gtggttttag aagatgtggc agcagaaact gttgtcggtg gaatacctgc
     6661 acgtgtatta aaaataagtg atgatcagac aaaagaaaat acagcattga ttgctgcgtt
     6721 acgcgaatta tagaggagat ggagaaaaat gcgcgaaata gatacctgta ttgtgggggc
     6781 aaccggatta gtcggccaaa cgatgctcaa agtgttagaa gaatatgatt ttccagtagg
     6841 acgattgaaa ttattagctt caaaacgttc agccggtaaa gtttgtgtat tcaagaaaaa
     6901 gaaacacact gtacaggaat tgacagaaac ttcatttgaa ggatatgatt tagcgctatt
     6961 ttctgcaggt gcttctgtat ccaaagagtt tgcgccaatc gcacgaagtc agggactatt
     7021 tgtcatagat aattcctcag catggcggga agattcggat attccattaa tcgttccaga
     7081 ggtcaatctg gaagactata cattgaatcg gctgattgca aatccaaatt gttcaaccat
     7141 tcaagcggta cttccgctta aagctttaca agaagccttt ggtatccagc gagtcaatta
     7201 ttcgacctat caagcagttt ctggttccgg gcaaaaaggc attgaagatc ttcatcgtac
     7261 gcaaaatgac gagaaaccag ttttgtatcc ttataatatt agccaaacgg tgattccgga
     7321 aatcgatcag cctttggaaa atgggtatac aaaagaagaa cagaaaatga taaatgagac
     7381 ccgtaagatt ctgcatcagc ctgctttgcc agtatcagca acctgcgtac gtgtgccaat
     7441 cgaaaatgga catggagtct ctgtggcagt tcaactgcaa aaagagttta cgacggaaga
     7501 aattagagag tgtctcagcc agtttccagg gattcaatta gaagatgatc tagcagcaca
     7561 taaatatccg acgagcattc ttgctagagg aacagatatg gtttatgttg ggaggatcag
     7621 aaaagacact tctttagaga acggattgtt gttctatacg actgccgata acatacgcaa
     7681 aggagccgct gcgaatgccg tacagattgc tgcagcttta atgcagaaaa tagcggaggt
     7741 ggcaagatga gtaatacgct gaaagtagcg aaattcggcg gaacatcgat gtctcatgaa
     7801 gagcaattca aaaaagtccg tacaattgtt ctttcagatc ccaatcgcca agtgatcgtt
     7861 gtttctgctt taggcaaacg tacgtcagat gatgacaaag taacagactt actttatttg
     7921 atttatgctt accttcagca tcatgttgat tggacacccc tttggaaaag gatttgtgat
     7981 cgtttcattc aagtaagaga tgttcttgcc ctgcagttac ctattgaaga tgaactagca
     8041 cgcatacaga aacaactgga agacgaaaag ataactgaag attatttagt aagtagaggt
     8101 gaatatctga ctgctcaact gatggctgag tatcttggtt accagtttat tgacgcaaaa
     8161 gacctgattt tctttgacta tgatgggaaa atcgatcagg aaaaaacagc acaggaaatc
     8221 acaaaagcat ttaaaagata tccgaaaatg gttgttccag ggttttacgg ggcgaaccct
     8281 gcaggaaaag aaaaattatt gggccgtggg ggaagcgaca ttaccggcgc gatactggcg
     8341 aagatgttgc aagcggaaag ttatgagaat tggacggatg tttcaggaat catgatggct
     8401 gatccgcgaa tcatcgatca tcctaaaatc atcgaggaaa tctcttttag agaactgaga
     8461 gagatggctt atatgggggc taacgttctt catgaagcag ctgttttccc agttcaagag
     8521 gctgatatcc ccatccaaat aaaaaatacc aatgatcctt tcgctcaagg gacaaaaata
     8581 tcgaatcacg aaatagaaaa ggaaaatgga ctgacaggaa tagctggacg caaggatttt
     8641 ctttccatca ctttattcaa acgtcatatg tccgatgaaa tcgggtttat ctggaaagca
     8701 atgagcattt ttgctaaaca tgagatatca atcgaacata ttccttcagg gatcgacaat
     8761 atcggagtga tcgtctcagc agaagcgatt gccgataagt tatttctcat cacgaaagaa
     8821 ttaaaagaaa cacttggcgt ggaagaaatc gtcgtgatcg aagacttagc tttgatctct
     8881 gtcgttgggg gtccgcataa agaattgatt ggattatctg ggaaagtgtt atcgatattg
     8941 aacaaattag aaattcgcac atcgatctta tcacaaggag cacaggagtt gaatctgatc
     9001 atcggtgtac cgaataatca atatgaaaca gtagtgaagg gtatctatga agggatggtg
     9061 aatacggatg ctgaaagaat atcgaatggc actccatcaa attcctgaaa tcgggtttca
     9121 tgaattcaag acaaaaaaat atttatatga acaagtaaaa aacagcgagg ccgttatcca
     9181 tgaaatcggc gaaacaggac tactccttta ttttgataat cagcaagaag aaacgattgc
     9241 ttttcgaaca gatatcgatg cactgccagt caaagaagaa acgaatctct cttttgcttc
     9301 taagcatgaa ggttatatgc atgcatgtgg tcatgacgga catatggcga tgcttttagg
     9361 gttgactgac tttataacta aacacattga ggatcttatt tataatgtag tactgatcta
     9421 tcagccatca gaagaaattt ccggtggcgc acaaagcgtt attcagtcag gattgttgga
     9481 taaatatgaa gtacaagcaa tatttggctt tcatctatgg cctggactag ctaagggaga
     9541 agttttctct cgaccaggac ctttgatggc acaaagcagt gaaacagaca ttatcattaa
     9601 aggccagtct gcacatattg cttctagtga aaaaggaatc gatagtctgg aggcagctgt
     9661 acgatttttg aagaatgttt atgattttga ccggcaatta ccggcagagt ggaatcatct
     9721 aatgaaattt ggccaaatcc aaggtggaac gatccgaaat gttttagctg gagaagttat
     9781 tgtatccgga agtatccgtt cttacagttc agatatgcaa aattatttga agaggggatt
     9841 agaccattta gcagaacagt ttcactctac agctactatt tcttttcgct ataatgacgg
     9901 ctatccagct gtaatcaacc atagaggctt atatgcagca ctgttagatt caaacaaact
     9961 acatgaatta gatgctccag ttcttcaagc cgaagatttt ggcatatata cgcaacaata
    10021 tccatgtgta tttttctttt taggtgtcgg tgatacccct gctttacatg atccgacgtt
    10081 cgattttgat atggatgttt tagaaaatgg tgtagaatgg tacaaaacga ttctttttac
    10141 agaaaatctt cttgtaaaaa tcgatagcag ttcaaaagag gagcgagtaa ataatgatga
    10201 atttatccaa tcgatttagt cctaggcttt ctagaataga agtatcaaaa atacgacaat
    10261 tcgatcagca aatatcgtcg ataccagatg tgatcaaact caccctggga gaaccggatt
    10321 ttccaactcc tgaacatgtc aagcaagcag gaatagcagc gattgaagaa gacttttctc
    10381 actataccgg aatgagagga ttagaggaac tgcgggaagc ggcttgtata tttcagcaac
    10441 agcgttatgg attaacttat gatccgcaaa cggaagtttt gacgacagtt ggtgcaacgg
    10501 aagcgattgc ctctgcgttg ttatctgttt tggaagaagg agacaaggtt cttattccag
    10561 cgcctgctta ttcaggatat cagccattag tggaactagc tggtgctgaa ttgatcccga
    10621 tagatacttc tgacacaggg tttgtctgtc agccggaaca atttgaacga gcatttgaac
    10681 aatatggatc agctgtcaaa gcagtcattt taaactatcc taacaatcct actgggacga
    10741 cattgtctgc aaaacaattg aaagcaatcg ctgaggtttt aaaaaaatat ccggtatttg
    10801 ttataagtga tgaagtatat gctgaattga cttatagtgg aacgcatatg tccatagcta
    10861 cttatcttcc agaacaaaca atcgtgataa gcgggttatc caagtcacat gcgatgactg
    10921 gttggcgagt gggctttatt tttgctcaaa aagcccttat tgacgaacta attaaagtcc
    10981 atcaatatct ggtgactgcc gctacaacga tgtcgcaaaa agctgcggct gaagcgctaa
    11041 taaatggggt gaaagatagc gagaatatga aagagcaata tcaaacacga agagactatc
    11101 tggtccagca attggcgcct ttaggatttg aagtgaccca gccaaacggt gcattttact
    11161 tgttttgcaa actcccagct gccattcaaa ctgactcttg gcagttttgt gtagaattag
    11221 cggaacaggc aaaagttgcg tgtatcccag gaattgcatt tggcccagaa ggagaaggtt
    11281 atatccggat ttcttatgct agcagcatgg aaaaattaca tgaggcctgc aaacggatca
    11341 aagacttttt aacagaacag tgattcatag attttatcaa aaaaacaatc gttaattttg
    11401 cggtatattc tgcaaggaga cgattgtttt ttaaatttag ttcaaatgaa cgcgtttctt
    11461 ttgattcata actaatgtaa tgttaagttt aaattgttta gaatgatttt aaaagaatat
    11521 tcaaggaggt ttcatcaatg gaaaaaatca aagttgtcat tgtaggtgca tctcatggag
    11581 ggcatcaatc gatcttggaa ctattgacac gatacggtga aaatgtagat atcacgctat
    11641 ttgaagcagg agactatatt tccttcatgt catgtggtat ggagctgtat ttagaagaca
    11701 aggtaacaga tgtaaatgat gtacgtaatt tcagaccaga aaactttccg caatccaatg
    11761 ttcatatttt aaataatcac gaagtaaaaa cgatcgatgc cgataaaaaa acagtaacgg
    11821 ttactagcgc atcagataac gaaacaaaag aatatcctta tgacaaattg attttgagtt
    11881 ctggtgtcaa accaaacaat ctgccagtac caggagcaga tctagaaaat gtctacttga
    11941 tgcgtggata caattgggca accaaaatca aagaaaaatt aaatgatccg gctattaaaa
    12001 aaatcgctgt cgtaggtgcg ggttacattg ggatcgaagc agcagaagca agccgaaaag
    12061 ctgggaaaga agtcgtattg cttgatgtga tcgatagacc tttaggcact tatttagatg
    12121 ctgagatgac agatatttta gaacaacacc taaatgaaaa cggtattgaa gttttgacaa
    12181 atgcaaaaat cgaagctttt actggtaacg gaaaagttga ggcaatcaaa acaagtgaaa
    12241 aagaaatccc agcagatcta gtcattcaag ctgccggtgt gaaggcaaat acagaatggt
    12301 taaaagggat cgtcgactta gatgaacgtg gctggatcca aacaaatgaa tacttgcaaa
    12361 cgaatcttcc agatgtgtat gcagtaggag atgctacact tgcttattct atcccagcac
    12421 gcaaaaaaat gccgatcgct ttagcgactg ttgcacgtag agaagcacgt tatgtagtaa
    12481 aacatctttt tgaagaagta ccaaacaaac cgttcggcgg agtcgtaggt tcctctgcat
    12541 taagtgtctt tgactatcat tttgcagcaa gcggattgaa tagctttaca gcagcaaaag
    12601 caggagtaga tgtacgagtg tcttattatg aagatacgtt acgaccaaaa tatgtaccag
    12661 aagaacaagg aaatccaaaa gtgtccatcc aattattctc ggatccaatg acccatcaat
    12721 tgctaggagg agcagtatta tctacctatg atgttacagg tcaaggaaat gtattggcac
    12781 tagcgatcca aaacaaactg actttagaag aactagcaga agctgacttc ttcttccagc
    12841 caggctttga ccgacaatgg agcatgttga atctagccgc tcaacaagcg ttaggagaag
    12901 aaccatttgt agaataagaa aacgaaatat ttgaaaagag gttgtgaaaa agctgccttg
    12961 catcaagtaa taagaacagc caagtaaaaa cagcttctta tgtttttaac ttggctggac
    13021 ttattaccgc agatgcaagc ttttgaacac cgtttatttt caaaaaaggg actgtgacac
    13081 gatttgtgta ccgaccccca aaagttagac caaaaaatct aacttttgga ggtcggtatt
    13141 ttttatggtt aaatatactt ttgaacttaa attaaagatc gttcatgatt atttggatgg
    13201 caaaggcgat tggaccgtaa aaatccagat caagagattg aagcggaaat gcttaaaatt
    13261 cgggagaagc acaaggatta tggctgttta cggatgacca atgaactaag aaaccgcggt
    13321 ttctctatca ataaaaagaa agttcagcgc ttgatcaaga agctaggaat aaaagtaacg
    13381 gcatacacgc gtaaatcacg tcgctacaat tcttatcgag gaaaagt
//
LOCUS       DAFEOM010000069        13395 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.072, whole genome shotgun sequence.
ACCESSION   DAFEOM010000069
VERSION     DAFEOM010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.072"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(14..706)
                     /locus_tag="LP524_002222"
     CDS             complement(14..706)
                     /locus_tag="LP524_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBL2235247.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1143..1712)
                     /locus_tag="LP524_002223"
     CDS             complement(1143..1712)
                     /locus_tag="LP524_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBL2235248.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="LP524_002224"
     CDS             complement(1786..2109)
                     /locus_tag="LP524_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235249.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="LP524_002225"
     CDS             complement(2263..3075)
                     /locus_tag="LP524_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235250.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="LP524_002226"
     CDS             3429..4277
                     /locus_tag="LP524_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBL2235251.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="LP524_002227"
     CDS             4422..5363
                     /locus_tag="LP524_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HBL2235252.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREIPFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="LP524_002228"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="LP524_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7636..9807)
                     /gene="copB"
                     /locus_tag="LP524_002229"
     CDS             complement(7636..9807)
                     /gene="copB"
                     /locus_tag="LP524_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HBL2235253.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9827..12013)
                     /locus_tag="LP524_002230"
     CDS             complement(9827..12013)
                     /locus_tag="LP524_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBL2235254.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="LP524_002231"
     CDS             complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="LP524_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HBL2235255.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12235..12675)
                     /locus_tag="LP524_002232"
     CDS             complement(12235..12675)
                     /locus_tag="LP524_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HBL2235256.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12749..13288)
                     /locus_tag="LP524_002233"
     CDS             complement(12749..13288)
                     /locus_tag="LP524_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL2235257.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3761 a         2810 c         2382 g         4442 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatccc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggttcg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaag
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag tcacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgggaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgga tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagttttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaatgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tggaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacctttttt cattattatc tgattttcaa tgtcaaagca tttagagcta caactaccgt
     7681 acttaacgac atcaagacag caccaactgc tggacttaat atgataccaa ttggcgcaag
     7741 aattccggct gctagcggaa ttgcaagaat attgtaacct gccccccacc agaggttctg
     7801 gatcattttc tttcgcgttt gcttggctag atcaagaaaa tgcaaaatat cttttggatc
     7861 gctgtttgtc aatacgacat ccgcagaatc gattgcgata tctgtccctg caccgattgc
     7921 catcccgatt gaagcacggg ctaaacttgg cgcatcattg atcccatcac ccaccatgat
     7981 tacttttttc ccttggtcaa ggtatttctg gacaacagct tctttatcat ctggtaataa
     8041 accgccataa tattcttcga tccctaaata atctgctact gcttgggcag cgtttttatt
     8101 gtcaccagta agcatgacag gagtgatttt tctttctttc aatgttcgga taaattcttt
     8161 tgcttctgtt tttactacgt cacctaaagc aacgattgca gctagatgat tttctaaaat
     8221 caggaagctg atggtatttc cttgttcttg gtaaggtttc aataatgctt gttctacgtt
     8281 taatccgaga cgctcaactt ctttttcatt aactattttc acttgttgtt ttttgactgt
     8341 tgcctctagc ccgactcctg aaagattttt tagattttca gcttgataag gcttgatttc
     8401 atgttctttc aaataattca taatgccaac agccaatgga tgattggcat tcgcttccaa
     8461 ggctcctaga tattgaagga tttcattttt attgaacgca tcatcaagta cttcgacacc
     8521 cgttacagta aattttcctt ctgttagtgt tcctgttttg tccagcatga tcacatctag
     8581 atcattggct tgctccagtg cattgcggtt tttcaaaagt aatccgtttt tagctgctat
     8641 tgatgtggaa cgagcaacaa ctaatgggat agctaaacct agagcatgag gacaggcgat
     8701 gataaagact gttaccatac gttctaatgc attaggaaga tctgtcaaga acaaccaggc
     8761 gatgaatgcc aggataccca caattaatgc gacataaaac agccattttg ccactttgtc
     8821 agataatgac tctagcttgg atttttctcc ttgtgctttt cgcaccattt ccataacctt
     8881 cgctagataa ccattttcac cagttccagt tacttcgatt tcgatcgttc catccccatt
     8941 gatagaaccg ccaatcactt tgtcattggc ttgttttttg attcctcttg attcgccagt
     9001 cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt acaccatctg ttggcatttt
     9061 atcaccagac cggacgatca aacgatcacc ttcgtttact tctttcaatg aaactgtctc
     9121 ttcttttcca tctttcgtca agcgtttcac tgattctggc agtaattctg ctagtttttg
     9181 taaagcattg ctggcattcg acacagcatt catttcaatc cagtgaccta gtagcataat
     9241 aacgatcaaa gtagccaatt cccagaagaa atccatgacg tgtgtatgtg gattgagcag
     9301 atttgcgata aacgaataga cactataaat atatgcaact gtaatcccca tcgcgatcag
     9361 tgtcatcata gcaggacttt tttgtttcaa ttccatctta gctccactta aaaatggctg
     9421 accgccatag ataaatagta tagttgctaa gatcaatacg agccaatcag aaccaggaaa
     9481 cgttacttga aatggaaatt ccattcccat cattggtgaa aaaagaatga ttgggatagc
     9541 taggataagc gatagccaaa atttttgttt gaaattcccc atgtgcatgg aatgatccat
     9601 gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt tgtttgctgt ttgctatatc
     9661 tccatggtta tgtcccatgt gcatggaatg atccatctga ctatggtcat gtttcatctc
     9721 catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt tatgctcatg
     9781 ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac tttttcatgt
     9841 gtcgatttaa gcacagggag ttcaacaata cactgacgga actaaacgcc atcgctcctc
     9901 ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc gtattataga
     9961 caaaagccca gaaaagattt tgtttgattt ttctcaatgt caacctagac aactcgattg
    10021 tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac atcggctgtt tccatcgcaa
    10081 tgtccgttcc acttcccatt gccataccga catcagctaa tgctaaggcc ggtgcatcat
    10141 tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt tctacgtagg
    10201 ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata ccaacttgtt
    10261 taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca tctaatcctt
    10321 tattctggag ctgcttgatt gcttgtttga cttctagttt gatttgatca gccacggcaa
    10381 tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc tgctgttcta
    10441 attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt aatcgtttac
    10501 gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca cctgggtggg
    10561 ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat tccacgattg
    10621 ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat aggacttctt
    10681 tagaaccgac tagatctgta actttgggct ttccttctgt aatcgttcca gttttatcca
    10741 aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta attaaaattc
    10801 catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct aaacctaatg
    10861 cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt tgccaatcac
    10921 cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg atcggaacaa
    10981 aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct tgagcatctt
    11041 ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgta acttccatgt
    11101 gaagtagacc attcgtattg attgtcccgc caaagagcgt gtcatccgga ttcttttcta
    11161 caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct gaaataattc
    11221 gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca acgactactt
    11281 cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt tttgtttgaa
    11341 gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt tccaaatatt
    11401 tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga tcttgggggt
    11461 gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt ccaattgcta
    11521 ccaatacatc catatttggt gctttcgtct tgattgcatg gtacgcacct ttataaaaac
    11581 gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt ggaaaatgaa
    11641 aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac aacatcaacg
    11701 gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa tacgcttgct
    11761 tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca tagccgatgt
    11821 tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca cttgcttttt
    11881 cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc tctttttcga
    11941 tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc attttggttt
    12001 ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg cttgacaaat
    12061 cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt ttttcaaatt
    12121 tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc ttgctgcaca
    12181 atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt cctcctattg
    12241 atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat ctactggttg
    12301 cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag tggcttcttc
    12361 tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa aaagattttc
    12421 cgtcgcactg cgaactgttt ccatctccga gacagctgga taataaatga atttttttcc
    12481 ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag ttttgatcgt
    12541 tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg ctgtcgtctg
    12601 tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactgatct ttactggcgt
    12661 tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt atcttatgaa
    12721 tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt ttcaatttag
    12781 ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt cttgcattag
    12841 ctacttggat cagctgctgt attttcggtt cattgttttc ttcaaaatgc cagataaaag
    12901 ataaaaattc taagtattcc ctgtttagtt tctccgaaaa tgattcaggc atatctggcc
    12961 gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag atggcgagat
    13021 aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc tctaatgtag
    13081 aagtataatt tccttcaacg atccatgacg gattagatac cataaacaat tgctgttctt
    13141 gcaaaaacca cttttttgcc tgaatactgt agtctgttgt atgccataaa aggtctagcg
    13201 gcagtagcgg aaagtcggtg atttctttga tcttccttgc gagggtcgat tttcccgacc
    13261 cactttgtcc aatcacgatg atcttcattc ttctccctca cttctctttg gaatttggtt
    13321 cgatcatacc acttataacg ccgcaaaaaa acaagggact gtgacaaaag tcgtgtcaaa
    13381 gtcccttgtt tcgaa
//
LOCUS       DAFEOM010000070        13345 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.073, whole genome shotgun sequence.
ACCESSION   DAFEOM010000070
VERSION     DAFEOM010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.073"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(44..1363)
                     /locus_tag="LP524_002234"
     CDS             complement(44..1363)
                     /locus_tag="LP524_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL2235258.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1360..2013)
                     /locus_tag="LP524_002235"
     CDS             complement(1360..2013)
                     /locus_tag="LP524_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2235259.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2301..2821
                     /locus_tag="LP524_002236"
                     /pseudo
     CDS             2301..2821
                     /locus_tag="LP524_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2723..3439)
                     /locus_tag="LP524_002237"
     CDS             complement(2723..3439)
                     /locus_tag="LP524_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBL2235260.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(3489..3698)
                     /locus_tag="LP524_002238"
     CDS             complement(3489..3698)
                     /locus_tag="LP524_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235261.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            3975..5248
                     /locus_tag="LP524_002239"
                     /pseudo
     CDS             3975..5248
                     /locus_tag="LP524_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5355..6386)
                     /locus_tag="LP524_002240"
     CDS             complement(5355..6386)
                     /locus_tag="LP524_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2235262.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(6393..7223)
                     /locus_tag="LP524_002241"
     CDS             complement(6393..7223)
                     /locus_tag="LP524_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2235263.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(7220..8035)
                     /locus_tag="LP524_002242"
     CDS             complement(7220..8035)
                     /locus_tag="LP524_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBL2235264.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(8032..8856)
                     /locus_tag="LP524_002243"
     CDS             complement(8032..8856)
                     /locus_tag="LP524_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBL2235265.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8846..9946)
                     /locus_tag="LP524_002244"
     CDS             complement(8846..9946)
                     /locus_tag="LP524_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBL2235266.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            10124..10909
                     /locus_tag="LP524_002245"
     CDS             10124..10909
                     /locus_tag="LP524_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HBL2235267.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10942..11325
                     /locus_tag="LP524_002246"
     CDS             10942..11325
                     /locus_tag="LP524_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBL2235268.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            11401..11532
                     /locus_tag="LP524_002247"
     CDS             11401..11532
                     /locus_tag="LP524_002247"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL2235269.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            11501..11737
                     /locus_tag="LP524_002248"
     CDS             11501..11737
                     /locus_tag="LP524_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL2235270.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(11815..12786)
                     /locus_tag="LP524_002249"
     CDS             complement(11815..12786)
                     /locus_tag="LP524_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBL2235271.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         4054 a         2912 c         2306 g         4073 t
ORIGIN      
        1 taagtctcta tttttcaata actattgcaa aattatatct taattaagaa aatattttta
       61 ttttaaagat aaaaaattct tcatcctgca atactttata ttctatattg taattattca
      121 gaatattact tacaatatat aaacctaatc cattactatt ttccttattt aaatcaaatt
      181 taacatcaaa tattttatcc atattcgaaa ttttattgtt accgtatgaa ttctctatat
      241 ataaccaatc attaactatc ccaatattaa ttaccccatt tacatcagta tattttaccg
      301 cattactaat caaattagat agaataatct ttaaagctgt ttttcctata taaacattct
      361 cgtctaatat ataattattg acagttattt tcttttgatt tgctaatatt tcatactttt
      421 ttaatatgtc ttctaatgta tcatttattt tcaaatattc ttcatcatta ttcaaatttt
      481 ctatagaatg aactgataat atttgagaaa tattttttgt taaactatca acgatttcaa
      541 tacattcatt aatataaata tctctttctt tatattttcc aatattgtat ttcatgtttt
      601 ccagtattat tttaagacta gcaagagggg ttttaagttc atgggatgca cctttaaaaa
      661 agtcatattt taacttttct aattttaaaa tttctttatt tttaaattcc aaatcgtcaa
      721 ttgttcttaa taaagtagaa tacaaatcat taatttgttg ttttaattct ccaacttcat
      781 cattagagct aacttttaaa ctcatttttt tatcaagttt catcatttta tcagtaacaa
      841 tttttatttc ttgtatatta ttttttattg attttgcata aattaaagaa ataatagcag
      901 aaaataaaat tgatattaat aaagaatatg gtaaaaattt aaggcttaaa tctttcgcat
      961 ctttttgcat atcagctgta gaaacaaatt gtagatttgt ttttttacca tcattaagct
     1021 ttatttctct ttcttcaatt atcaaagaat tactatcact ttctaaatta acatttatat
     1081 tatctttgat ttgtatttca ttcttattat ttttttcttt tatgaatact tttatttcac
     1141 tactcttgga ataaaactcc aaagtttgtt ctatatattt tatttctttt ccattcatat
     1201 tactagaaat ttcatttgcc atattatgaa tcttttcttt tctcgtctcc aaatatgttt
     1261 taggaaagat aaaaaaaatt aatgaatgaa ctaatataat tattattcca agaacagaaa
     1321 atatttgtat gaacattttt ggaaatatct ttaatttttt cattttctct ccaatttata
     1381 tcctacattt cttatagtag ttatacagtc taattgtaat ttttttctaa gttcttttat
     1441 atatacatct attactcgat cataaggagt ttcttcgcta tctttccata cataatcaat
     1501 aatttgcatt cttgttaaca cttgtccatt attatccaat aaacatttta atacttctag
     1561 ttcttttgca tttacatcta tttcttcacc atttatttta gctgtatagc tattaaaatt
     1621 aaccgataga tccttatatt caaacttctc taaatgtcca taattcttct taattaaaga
     1681 atctattcta gcttttaaga ccggcaatga aaatggcttt tctacatatc cgtctactaa
     1741 attagtaaat gcatcaattt tatattcttc atcactaaat gcagtcaaga ttagaattgg
     1801 taaattgctt ttctttctaa tttctttcaa cacttctaaa ccatttataa aaggtatctg
     1861 aatgtctaag ataaccaaat ttatatcgct attaaatttt gacaaggctt ctcttccatc
     1921 tttagcttga ataacagtat atccaaattc tgaaagatat tcacttattc cctctcttat
     1981 catattatca tcttcaacag ttaatatttt catatatacc tccacttaag cctatacact
     2041 agatattata cagcatatta tggctctata atatctagtg ttggtgacgc cttctgttgc
     2101 caatactttt tctttttttt aataaatgca aacagacacc ttgaaatcct ttagaaaaaa
     2161 ggcttctata atattttgtg ttgttggaaa atcgaaggcg attttctact aacacttttt
     2221 cttcgtttca actcaaaaag agactcactt ttggtaaaat ttagtcgacc aaaacatcac
     2281 cctaccaaag gagtcgctct atggataatc atactagaaa attactcgga ttaacagata
     2341 aacatttatt ttttgatgaa gaatggctga ttgaaaaaga atataaaggc gttcaagcgc
     2401 agtttattaa agggagattg gatgactcac cttcccattg tgaacactgt ggatcgattc
     2461 gtattatccg caacggttcc tatacgacac gtactcagat gttgaaagtc aaagaaaagt
     2521 tgactatttt agaattaaaa cgtacccgtt ttctatgcta tgattgtggg cagacctttt
     2581 ctgcgaaaac ggacttagtt gatgaacatc atcaactaac aaaggaactg aagcaggcga
     2641 ttctcataga actgtacgaa aatcaatcac gtaatctgaa aatcagaaga agtaccgtcg
     2701 gctcaagcga tattggaaac tcttactcaa agattccacg acattggagc cgctcaaacg
     2761 gcattatcat cgactgttta aacgaccaat tagtcaaaca gaaatcgtcg atgaaccgtt
     2821 gtgcacaatg gtgtcctgaa cgtacagcga caccttccat atccagagca gtcgccacat
     2881 cacggggatg gatgccatcc aaattaaaag caaccacgcc agtatgatgt tcaggatctt
     2941 gcggtccgta aatggtgata ccgccgatcg cttgtaattt aggtaaaacg taggcaacca
     3001 gttcttgctc ataacgatga atattctcca taccaatctc attcaagtaa tcgattgctc
     3061 ctgcaatgtt cggagtacca gcttcaaatt tccatggcag ctctttccat gaactatcgt
     3121 aaagattgac aaaatctatc atttcaccgc cgaattcgac tggctccatt tgttctaaca
     3181 attgacgttt gccatataat acaccgattc ctgtcggccc gcacatttta tgcccgctaa
     3241 aggcgaaaaa atctgcatcc aacgcttgta catcaactgt catatgcggt gctgcttggg
     3301 caccgactgg actatcctca actacaacag cctgttcaac cctcagccgt ttcaagcaca
     3361 acaaataacc ttctggatct ggtttgccga cctgaagatc atcgatcgtc acaaaggaat
     3421 caattttttc cttaatcatc ggatgtgctt caaaaaaata gtctagctcc cgcagttcag
     3481 aattgagact actccgattt tccagtgctg ctgatccgct gctcaaaatg ttcagcatcc
     3541 actggtctcc caaccgcttg acttatccct tcataaaagg acagttctgc tgccgtccca
     3601 acaccttgtt gatagtctgc aaaaggaatt ttatagccga actgctccag taaataggct
     3661 tcagtaaata gtgatgaata ctctcgtaaa acatccattc actgtcaatc atcgtcccat
     3721 caaaatcaaa aataagtgct ttggtcatct tctttccccc ttacccaaaa caagcaaaag
     3781 ctgtgtcctg ctctctctac agtatcagca taaaaactgc cgtctgaaaa gtcaaaggtg
     3841 aaagaaatgt ggcgctataa tttctaggac tgatagtaaa tcattctatc agtccttttg
     3901 agcataaaaa aacaaaacat acagaagctc cgttagaatt gaatcaccac aacacaatca
     3961 aaggagcgtt ctgtatgcct gatcttcatc ttatcaaact attactagga atcaaagatg
     4021 gaaacattca aatcactcat gtcgagaaga aaaaaataca ttcaatcaac agtatcgttg
     4081 tttctgccac gctcattaaa acgattcagc cctgtcctca gtgtaaacag gtgaatcaac
     4141 atgggctgat cgttaagaac ggaaagaaaa aatcgctggt ccttctcaat aagtgtgcga
     4201 atcaacggac ttatcttgct ttggcaaagc aacggtacct ctgtcgtggc tgtcacacct
     4261 attttacggc gaatacctac attgttgaaa aaaactgttt tatcgccaaa caagtacatt
     4321 ataaaatatt ggaggaattg actgaaaaac aagcaatgac gacgatttct aaacattgcg
     4381 gcgtttcttg gtcaaccgtt tcgcgaacac tcgcttctct gctccccatg acaaacgtga
     4441 aaataaattg gttgcctcgc tgttcattgg tagatgaatt tcgttcattg aaaaatcaag
     4501 tcggtcccta ttctttttct tgtatggatg gcgatactgg gaaattactg gatattcttc
     4561 cttcacgtaa gaagaaagat ctcatttctt attttatgca atttgaacga cgtgcacgtt
     4621 taaacgtaaa aatactcgta acagatatga acgcctctta tgctttgctg ataaaagagt
     4681 gctttcctca tgccaaactc gttgtggatc gctttcacat cgtcaagcac ctcattcgca
     4741 gttttaagga tatttgacta tgagtcatga aaagcttcga ccgaaacgat ccaatacaag
     4801 ccaaacacta tcgacaagtg aaggctttat ctcgacttct gatcaaacga caggacacgc
     4861 ttgtttatga taaatggaca aaatggcgta attttggttg ggcctattta accgaaagtg
     4921 aagtcgtaga acgtctactt tctacttctg atgaactacg aatagcttat gcttactatc
     4981 aagaaattct tcaagctttt tatgacaaag aagcggacat cttctttcag cttgtaaaaa
     5041 caatgcccaa gtcggttcca agagaactgc atcatataaa aaaagcgttt ataaactatg
     5101 aatccggcat tcgtttagcc ttagagttgc cttactcaaa tggcaaaata gaaaatctgc
     5161 atacccacat caaagcattg aaacgaatag cttacgggtt aggatgagaa ccagaatttt
     5221 cttaatcaat catctgatca cttattaaac ataaaaaaac atctaaagca gaaaatcaat
     5281 tctaccttag atgttcttat atcactctat cagtcctatt tgacaaagag ccagaaatgt
     5341 ttgccgacta tacactagct ttgttgtgca aaatgctgag tatcaatttc cacagggatt
     5401 gctagaatat ctttcagctg aatgtataag gcatcgctaa ttttctcttc ccgatcgttc
     5461 aaccgatcca ctaaaatttc tttgccctcc agcgtttgaa aaacataccg ttgaatgttg
     5521 cctaaaataa atgattcctt ccatagaacc ggaatcttaa ccccctgagg aaccggttca
     5581 aattgaatca gctccggtct taaataataa atgcagcgat tgcccttagc ggaaaactcg
     5641 tgggtatact gattcagctc ataccattcg aaacgattat agttgccgat aaattccgca
     5701 acaaatcggc tctgaggatt ctgatacaat tccttcgagg tggaaacctg agccacccgc
     5761 ccagcttcca tcacaaaaat ccgatcactg attcgcatcg cctcttcttg gtcatgagtc
     5821 acaaaaatca tcgtcatccc tagcgattgc tggatagcgc gcaaatcccg ctgcagctgt
     5881 tttctaattc gggcatccaa agcacttaat ggctcatcca gcagcaggac tttcggttca
     5941 gtgaccaatg agcgagccag tgccacccgt tgtcgttgac cgccagaaag aaactggaca
     6001 tttttatctg cctgctccgt catatccacc aatgatagca tctcaaaaac cttttcctga
     6061 atcacctcag gactcagctt ctttaccttc aaaccaaagg ctacattttc aaaaacagtc
     6121 atagttggaa acaatgcata ctgttgaaaa acaaagccga tattgcgatc cttcgtaggc
     6181 ttatcggcaa tattctcatt gtccagcaca atctcaccgc tctttggttg ttccaatccc
     6241 gtgatacagc gcagcaacgt agactttccg cacccgctgg gacctagtaa agtaaccagc
     6301 tcaccctttt caatctgaat ttcatcaata gctaaaatct gtttttcatc aaaaaacaga
     6361 ttgagatcat gtatttctaa aaacattttc cgtcacaact cctttaaggg cttctttttc
     6421 gactgcttct cttccctcgt ctgcttaagg gacagctgtt gcttttgtcg ataagtcagc
     6481 ataaaggtag ttaaagcaat tcccaccata aacagcaaat acaaaaccat cacagcgcta
     6541 gccaaatggc cgttttcgtt catccgccgc agcatataga tgcgtaccgt ctgaaatcga
     6601 ccgccaatca acaggttcgt cagcatgtac tccccaaaaa ccgaagaaaa aatcatcaga
     6661 gaaactaggg tcacaccgat acggatgttt ggaaacagca cctggataat aatcgtagac
     6721 atcggggcgc ccaaggtttc tgctgcatcc actaattcct tcagattaat taagcgcatc
     6781 tggttgttaa gactaagata gacaatcggc aacgccgtaa taaacaatgc acctaccaac
     6841 actacaaaca ttgggatacc agtttcagca tacgttcgaa gcaaagccgt taccaaaatc
     6901 actcctggta aagcatacgg cagcaggagc agcttttcca gccaacgatt caaacgaggg
     6961 aaatacaaat gaatccaaat aatggtggga atcatcaaca acagaatcgt caccaagacc
     7021 acacctgcta ataaaagtga gcgacccacc gcagcaataa attccctgtc cgtcagcagc
     7081 tgttggaacc attgcaaggt aaaatcacta ggtaaaagcg attttgacca tttagtggag
     7141 ctggcataca aaatcgttcc aataatcggc agcaagtaat acaggacaaa gcagaacaaa
     7201 atcggtttta ttaacttttt cacgtctgca ccaccttgcc tttcttttga ataatcaaca
     7261 ttgccgttac cagcaggaca cctaaagcga ctgccaacgc actgcctaaa ttaggcttgg
     7321 cgaacacatc tcccgacact aaagccgcaa ttcgaatcgt taaaagattt acattactgc
     7381 tggtcagggc atacgctgtt tcataggtcc ccatgccatt agcaaataag atgacaaagg
     7441 tgctggcaat cgatggtcgc aaaaaaggca aagccacctt gaaccaagca tacgctgatg
     7501 aagcgcccag caattgtgcg gactccagcc attccttttt cacttccttc atgctgggat
     7561 acaaaaacaa gacccctaac ggaatttgga aataggtata cgtgatggtc aatccccacc
     7621 aagaatacaa attgaagtcc gctagaaatg gcaaaatcat tttcagcaca cctacattcc
     7681 ccaacaaaat aataaaagag aatgccagcg gaatcccagc aaaattagct gccaaattgg
     7741 aaacaaaggt gattttctcc tgcaatttag tcgatagatt cattaagcat aaacataaaa
     7801 taaacgtccc gattaaccca atcacactgg agctgacagc aatcagtacg ctgttaacaa
     7861 aggcctgtgt gtaatatttg ttgctgaaaa tatcactgta attggccaat gtccactggt
     7921 tagttaaatc actgtgcaag ctgcacagaa ccataaaaag cagcggcagc agtaaaaatg
     7981 cacagaagat aaaggaggca ggaaccagaa ccaagcgttt tttcatctct ttcatcccct
     8041 catcaattct tgaatgtcct gcattttctc tgacggtgca attcctaata accagcaaaa
     8101 caatggagcc gccagcagct gtggtacagt ttgttccttc agcttttggc gtttatttga
     8161 aatcaaataa aaaggaactt cacgatgtgc agccaagctg ccgccatgca gtccgtgcgc
     8221 atccatcccg tgatccgcag tcaccacgac ttgataacct ttttgcagcc attcaggaag
     8281 gtaaatagcc aataatctgt ccgcctgatt aaccgctgct gcatattcat gagaatcggc
     8341 ggtaaatttg tgaccgacat catcaatatt catcgaatgt acgaccaaaa agtctggctg
     8401 atactgattc actaaataat gggcatccgc aaataaatgc gaatcagggt aagtgtcctc
     8461 actataaaag atgccatgct gaatcagtcg ctcctcctga tgctggatgc gatcaaccaa
     8521 acgattatag ggggcttgat tgtacaactc gctgtaccag tggtaagcgg cggctcctgt
     8581 ttttccgcca gcttccttaa ctaattcaaa cagcgaccac tctttggatc gctgagcatg
     8641 gtaattacta tagatgccat tttcataggc tggaacgccg gtcatcagta cttcgtataa
     8701 cggacgtgaa ttgctgggca gctcactttt taccttcaat aagcttgctt gctggtgctc
     8761 aactaaatga ttcatgaacc caagctgggc gacggctgtg tcgtagcgac agccgtctag
     8821 tataactaca atcagtttgt caagtctact ttgcataggt cagtacctcc gcctgccaaa
     8881 gctctggaag ctctacagag gtcttgttcc aagcttcatt gtcttcaact ggcaccgccg
     8941 cttcataagc atcctccggc agtaattttt cagctacttc tttaggcagc tctaccttgc
     9001 gaataggtcg cgcatagccc tttgccaaat tgatttgccc ttcatcagat aaaatatatt
     9061 cccgtgctag ctttgccgca ttgggatgag gggcattttt attgattaag gttgtatacc
     9121 cgctaatgat ggagccgtct gttggcacag ttacctcaaa acgctcggag tcaatttggt
     9181 cacgataatt tagtccattg aagtcccaaa caactgccac atcgatttca cctttttcaa
     9241 gatttgctac gctggcatca acggtggaca aacggccttt ttctgccatt tttttgtagt
     9301 aattcagacc tggctgaata tccttttcgt cacctccgtt ggcaatcgcc gcagccaata
     9361 cagcaaattg tgcttggttg gcaatcgtca catcccccac gctaacttga tagtcaccct
     9421 cttgcagctc cttccagcta gtcggtgctt gcttcacatt ctctttatcg gtgataaagg
     9481 aaatggttcc cgtatagctt aacaaccaat ccccatcatc atcctttgcc caatccggaa
     9541 tttcctccca atagttggtc ttataaggta aggttaactc tttgctttca gccaacggac
     9601 cgaagctgat accgacatca ccaatatcgg cggttccatt ttttccttct gattcaaact
     9661 tagccaattc ttctgcactg gacatatccg tatccacgtg acttaagccg tattcttttt
     9721 caaggtcgtt ccaagtgcct acccaatttg cccaagtatc cggcattccc aagctggcaa
     9781 tctctccttc ttccttggct ttttctataa tcgtatctaa agagtcgtct gccgcagccg
     9841 attgcttcgt tgcttcacct gagccgcagc ctgtcagcag gatacttact gttgctgcac
     9901 aaaataaacc gatagtcatg ttacgaattt tttcattcat tttcattcat tcttctcccc
     9961 tttcgataat tccattctaa caaagctttg taatgagaga atcagtttct tgaaaaggtt
    10021 tcatgaacaa tgtaaaggtt gtatatggtt tgtaaatttc gacgaattcc cgttaacctt
    10081 ctaaagcttt tgcggtatga tagtagaaaa aaggaggctc cctatgcagt attatgatca
    10141 gcatctccac acgtattttt cctttgattc agaggaaaaa tttgaaaact atttagccta
    10201 tcagccggag tttttcgttt ctacggatca ctttgattta aacaatccat ctaccggaaa
    10261 agatgacatt ccggattatc cagcctatat aaacaaactg gactctttaa aagaaaccta
    10321 ttccaccaaa tttctgcgag gtatcgagat tggcgttgtt ccaggacaag aagagcggat
    10381 cgtggattac ctcgctcagc atccttatga tttaaagctg gtaagtattc atcaaaatgg
    10441 ccgctttgat tatatggacg atgtggtttt aaccaaagat cccattcaaa ctaccaagga
    10501 atattttgat cagatgttgc aggtcttgac ggattttcca gaaggagata ttttgactca
    10561 ctttgattat ggattacgcc gcttcacctt cacagcggaa gaattcgcgg agcattttga
    10621 ggctcagctg ctaacgattt tcaaaaaggt tgttgaacga gaattggcga tggagttaaa
    10681 tgccaagagc ttcttgaaat acggcaacga agacctttac cgttatgccg ttcctttgta
    10741 tcaaagtgtc ggcggaaagc tctttacctt aggctcggat gctcatgttg ccgaagatta
    10801 tcagctgggc tttaaaaaaa tgagtcagct gttggcagac tgtcaggtag aaaagttgct
    10861 agtcattcaa ggaaaagaac gccgcctgac gccgctgccg attttgtgat tcctatataa
    10921 tagaagaaag tggtgaaaaa aatgagcaac ctgtgggact acaatcaaga ggcacccatc
    10981 cattatttaa ttgcgcgaca ttgggatgcg ttgaaaattg aggcggtttg ccgatccttg
    11041 ttagcagccg ttccaaaaca acaactggaa aattttttgg tggctgacag cttgcagcgg
    11101 gaaaaagttc aggcttattt tgcagccttt aaagatcagc ctttggaata tttgcatgcg
    11161 cagtttcatc ttttttatca agtagccgcc ccagatgact acaacgattt acgaggacag
    11221 ctgcaattga cctttcaagc ggatgaaacc gcctataccg ttttattggg aatggctcgc
    11281 cttggtgatc aggcgaaggt cgagtggcgg attttcgata tttgatggca ggatcaaggc
    11341 gtttcagttg caaaccttcc tctctttcaa ttagaaaaac cgcagcagga agaagtttgt
    11401 gtgctggtca ccgatgctgg tgaaattcgt ctccgcttat ttcctgacca agctcccctg
    11461 gctgtagcca actggcgctc cctagctgag caaggcgttt atgacaacac acctttcggc
    11521 tcgtgtcatt aaggaatttg ttattcaagg tggcgctttg gatggttctg gtgatgaagc
    11581 tgtttccagc tatgagggct ttttcgctga tgaagtgcat cgcggacttt atcattttaa
    11641 tggcgccttg gccttagggg accacggccc ccacaccaac ggcaaccagt tttttattgt
    11701 gcaaaacaca aaggcgcagg ctgacctgct aatgtaaagc aagcatactt ggagcttgga
    11761 ggactaccgg agctggatgg tcgttatact gtttttggac aggtatacca agggttagaa
    11821 atcgtagcag cgatcgcagc tcagccgaca aacagtgaag atgtaccgat tgagccagtg
    11881 ttcattcgac aaattaactt cgaaaaaaca tcgtaaaaag acactcactg acttcagcaa
    11941 aagctctatt cttgagctgg ctgcagctag tgagtgtctt tcatttatcc ataagataaa
    12001 gcttggattc aataaataaa taaatgtttt ggttaaagct gtgaccagca aatgaggaac
    12061 cgtgcgagcc agttgatctg ccaatgcaaa catttcttta ggtgaaggcg ctggcgtgtc
    12121 tgccaacgga tactccagtc ccaatgcttg gtaaccgtgc ataccatagc agtggtacgg
    12181 tagtaattct aacggtacga accgatcaag atctgccaca aaacaagcaa tcgtcagcag
    12241 ctcggtcagg ttcatattga tacctggaac cacaggaact cgaattcgca cttcgcagtt
    12301 aggatactca attaaaaagc gacgaatatt cttcaaaatt tgttggttat ctaagccggt
    12361 ccattgtttg tggagcttgc tggtaaacat ttttacatcc acaaacaatg tatccacata
    12421 gggagctacc ttttggattt cattataagc accacaaaag ctggtttcca gtacggtatt
    12481 gattccaatg tatttcgatt tttgcaaaat ttctttagca aaatccgcct gaaccagtgc
    12541 ctcgcctccg ctaatggtca ctccgccatc cgagtgaaag taaaaaacag catccttctc
    12601 aatctcatcc attacctctt cagcggtcat ggtctggcca aatccttttt caatctgctt
    12661 gctttgtgat tcgggaatcg aacaccaggc acagcgtaat gggcagccct ttaaataaac
    12721 aacggtccgt aaaccagcac catcatgtag agaaccctgt tctattctta atactgttcc
    12781 ttccatatag atttttgaat tacacactag tttaaactaa aacacacaag gaaaaactta
    12841 ccatttaatc ataaaattag ctcatataaa tatactagga tgtaaaatag actggtcaaa
    12901 atctggtcaa aaaagaggac taatacaggt agtcctcttt tttgttattt taatattcta
    12961 aatcattttt tattggttta ttcccttatt cgatgtagac tcaaatcact atatcactag
    13021 atatcaaacg gcgtcatacg gaatcaaaaa catcaaattt gacctgttaa atggtcattg
    13081 aataacattc aaaatcaata aaacggagtc agacacggag tcaaaaacgc tgagagtaaa
    13141 aaatatatac agccttgtag gtaaagtacc acagggctgt atatttttta tttcagatta
    13201 ccttaaatga ttccatttac tttttatctc attttttcct actagttagt atcaaatact
    13261 ctcctatttt ctcaataaac tatttttgga tacatggtat ctgaaacagc taaaaccttg
    13321 tcattacagc aaaaaatcac gttat
//
LOCUS       DAFEOM010000071        13335 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.074, whole genome shotgun sequence.
ACCESSION   DAFEOM010000071
VERSION     DAFEOM010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.074"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(178..735)
                     /gene="frr"
                     /locus_tag="LP524_002250"
     CDS             complement(178..735)
                     /gene="frr"
                     /locus_tag="LP524_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBL2235272.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(738..1460)
                     /locus_tag="LP524_002251"
     CDS             complement(738..1460)
                     /locus_tag="LP524_002251"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBL2235273.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1596..2477)
                     /locus_tag="LP524_002252"
     CDS             complement(1596..2477)
                     /locus_tag="LP524_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBL2235274.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2576..3358)
                     /gene="rpsB"
                     /locus_tag="LP524_002253"
     CDS             complement(2576..3358)
                     /gene="rpsB"
                     /locus_tag="LP524_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBL2235275.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3717..4196
                     /locus_tag="LP524_002254"
     CDS             3717..4196
                     /locus_tag="LP524_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HBL2235276.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4413..5504
                     /gene="phnW"
                     /locus_tag="LP524_002255"
     CDS             4413..5504
                     /gene="phnW"
                     /locus_tag="LP524_002255"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBL2235277.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5497..5625
                     /locus_tag="LP524_002256"
     CDS             5497..5625
                     /locus_tag="LP524_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBL2235278.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5629..6174
                     /locus_tag="LP524_002257"
     CDS             5629..6174
                     /locus_tag="LP524_002257"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBL2235279.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6628..8319)
                     /locus_tag="LP524_002258"
     CDS             complement(6628..8319)
                     /locus_tag="LP524_002258"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBL2235280.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8739..9686)
                     /gene="arcC"
                     /locus_tag="LP524_002259"
     CDS             complement(8739..9686)
                     /gene="arcC"
                     /locus_tag="LP524_002259"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBL2235281.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9801..10820)
                     /gene="argF"
                     /locus_tag="LP524_002260"
     CDS             complement(9801..10820)
                     /gene="argF"
                     /locus_tag="LP524_002260"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBL2235282.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10911..12140)
                     /gene="arcA"
                     /locus_tag="LP524_002261"
     CDS             complement(10911..12140)
                     /gene="arcA"
                     /locus_tag="LP524_002261"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HBL2235283.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            12601..13302
                     /locus_tag="LP524_002262"
     CDS             12601..13302
                     /locus_tag="LP524_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBL2235284.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT         3752 a         2710 c         2368 g         4505 t
ORIGIN      
        1 ttaaaagatt cccactatct taagtattat agcaatatct aactgttttt cctatgaaat
       61 cattgttttt ttatttaaaa actgaataat tttttctttt tttaattttt aaataaaaga
      121 aatataaata aaaacagagg aagcggaatg atccgcccct ctgtttttat gattttatta
      181 aacttccaat aattcttttt ctttatcagc agcgatctta tctacttctt tgatgctgtt
      241 atctgtcaat gtttgtacgt ctttttctaa tccgcgcaaa tcatcttcgg taatatcacc
      301 atttttttgt tgttttttat attcatcgat cgcatcacga cggatgttac gaatcgcgat
      361 ttttgcattt tctgcttctt ttttcacatc tttagctaat tcttttctgc gttcttctgt
      421 cagttgcggg ataactaagc gaatgacatt tccatcattg gttggactga ttccgatatc
      481 acttgccata atagcttttt caacatcttg aagaatgctc ttgtcaaaag gtgtgatcat
      541 taaaacacgt gcttctggaa tttggattga tgccatttgg ttcaatggtg ttggcgcacc
      601 gtagtagttc acagtaatac ggtccaaaag gctagcgttc gcacggcctg cgcggatttg
      661 cccaagttca cgttgaagat tttgtgctgc ttttttcatt ttttcttttg cttctgtcat
      721 aattgcatct gccattttta tttccccctt acagttgttc cgatattttc gcctaagatt
      781 gcacggcgaa tatttccgtg ttcatttaaa ttgaagacaa gcagtggaat atcattatcc
      841 atgcttagtg aacttgcagt tgaatccatg acttgtaatc ctttggcaat aacttccaga
      901 tgagtcagtt cttcaaattt tactgcattt gcatctactt tcggatcagc agaataaaca
      961 ccgtctacat tgtttttagc catcaggata acatctgcgc cgatttctgc cgcgcgtaat
     1021 gctgctgttg tgtctgtcga gaagtacggg ttgccggtac cgcctgcaaa gataacgatc
     1081 cgtccttttt ctaaatgacg ttctgcttta cggcgaatat atggctctgc gatttgacgc
     1141 atttcaatcg aagtttgcac gcgtgtcggt acacccacgt tttccaacgt atcctgtaaa
     1201 gctaaggcat tcataactgt agctaacata cccatgtaat cagcttgagc acgttccatg
     1261 cccatttgtg ctccgatttg tccgcgccag atatttccgc cgcccacaac gattgccatt
     1321 tcaacgccca gctcgtgaac ttctttgatt tcttggatga tttctttgat tactggtggt
     1381 ttgattccaa atcctgcgtc cccagctaaa gcttctccac tcaattttaa gactacacgc
     1441 tgatatttcg gtgtgaccat gttcattcct ccaatatttt ctattgccat tttaccatac
     1501 ttagatgatt tcgacactct aagactggtt tttcttgttt tgcttaaaaa aagggagcgc
     1561 acaaaccgtg cgttcccgtc tgttattcaa cagaattatt ttttcacttg gctcataact
     1621 tcttctacaa agttatcttc gcgtttttcg ataccttcgc caacttcgaa acgtacgaat
     1681 gttttaacag ttgcgccttt tgatgcaacg tatttttcaa cagtcatatc aggatcttta
     1741 acgaatggtt ggtccaccaa agcgatttct gctttgaatt tcttcaagcg tccttctacc
     1801 attttttcaa cgatattcgc tggtttgcct tcgttcaatg cttgttctgt aagaacagtt
     1861 ttttcatgtt ctaattcagc ttctggaatt tgtgattcgt ttacataacg aggattgata
     1921 gctgctacgt gcatagctac gtctcttgca actgtttcgt cagttgttcc atctagaaca
     1981 gtcaatacgg caatacgtcc gcccatatgc aagtatccac caaatgcagc attgtcgtct
     2041 ttttctacaa cttcaaagcg acggaagctg attttttctc cgataacttg agttgcttcg
     2101 atcaagtcag attcaattgt acctttatct gttttgattt tcattgcagc ttccatgtct
     2161 gctggtttgt tttcagcaac aagttctgca atttctttta ctaaatcttg gaacatttcg
     2221 tttttagaaa caaagtctgt ttctgagttt acttcaacga ttgctgcagt gttacctttc
     2281 acagcaactg aagccaatcc ttcagctgca atgcggtcat ttttcttcgc agcctttgcc
     2341 attccttttt cacgtaaaag atcaactgct ttttccatgt cgccttctac ttcaactaat
     2401 gcttttttcg catccatcat tcctacgcca gtcatttcgc gtagttcttt taccatttta
     2461 gctgtaacgt ctgccatgtt taactcctcc tgtgtgtttt tctttttaaa aaaagctgct
     2521 tcaaaggaga gtgaggcttg tcgcccagcc tttgaaacag ctccatcatt caaaattatt
     2581 ctgctgaaga attgtcgcct tcaacaacat ctacgatttc ttcgattgat gttgctgtgt
     2641 tttcttctgt gaaatcttct tcaactactt gatcttcacc ttggtttcct tcgatgaaag
     2701 catcagccat ttttgaagta atcaatttaa cggcacgaat tgcatcgtcg tttgaaggga
     2761 ttactacgtc gatttcatct ggatcgcagt ttgtatcaac catagctacg attgggatat
     2821 ttaatttgtg tgcttcttga acagcaatac gttctttacg tgggtcaacg atgtacatta
     2881 catctgggat tcttggcata tcagcgatac cgcctaagaa tttttcaaga cgttcacgtt
     2941 ctttgttcaa accagcaact tcttttttag gtagaacttc aaaagttcca tcttcttcca
     3001 ttgcattgat ttgtttcaaa cggctgatac gtttttggat agtatcccag tttgtcaatg
     3061 ttccgcctaa ccaacgatgg ttaacgaagt attgtcctgc acgagttgct tcttctttga
     3121 ttgcttcttg tgcttgtttt tttgtaccta cgaataatgc tacgccgcct tcttcagcaa
     3181 cgctcttcat gtaatcataa gctgcatcta ctaatttaac tgttttttgc aagtcaatga
     3241 tgtagattcc gtttctttct gtgaagatat atttcttcat ttttggattc cagcgacgag
     3301 tttggtgacc aaagtgtacg ccggcttcta gtaattgttt catagaaatt actgccatgt
     3361 ttgtttcctc cgatttggtt ttatttttcc tcttcttatc ttctaccttg ccggcaaact
     3421 ttttcaagca cctaccgaca atccaataag aatgtggatt tgctgatgca taaagcaccc
     3481 tcagctatta tacagacaaa agatccgtat cgcaagagca aatatacttt tctgctcaac
     3541 attcaatagt ttacctgaaa gataaaaaga attcaagaaa aacagtagat ttctctttgt
     3601 ttctttatta tcctttttcc ataaaataat tttaggtata aaagacgaat attattgggc
     3661 aatccttctt ttttttgtta tgatacatgt gaaatctttc gtaaaggaga aagaaaatgc
     3721 gcaaagctca aagacatttg ctgattaaac aagtcattga agaattcccg atccgtacgc
     3781 aagaagaact attaaacaaa cttgggcatt atggcgttac cgccacgcag gcaacgattt
     3841 ctcgagatat ccgtgatcta aaaatcgtga aggcacctga tgaaaatggc gtttcccgct
     3901 tcgtattgtt tcgcgaaaaa ggaactcctg agactaggac tgaggaagag gatcggctgg
     3961 ttcaaatgat tgaagacatt gtattgaaag tcgaacgagt ccactttttg acgatcgtca
     4021 ataccttgcc agacaatgct catttatttg catctgtttt agatgagatc aaacctccct
     4081 ctattgtcag tacaattgct ggattcgata cgatcatggt catctcggaa aatgaggagg
     4141 acgcaaaacg agtagaagaa tttcttcacc atccaactga agtatcactt ttttaatcag
     4201 agcacgtatt cctaatatgg aatgcgtgtt tttttatttt caatattttc cttattgaat
     4261 aaacctttac actctttaca aaaatacatc attttttatt caaatttttc aacaccttga
     4321 ttgaatcaca ataatcatca tttatattgg atgttaacaa gagcagcttc ttgctctttg
     4381 tgtatttcaa aaaagaaatg aaaggaaatc atatgtataa attattaact tctggaccat
     4441 tgacgacttc tgaaacagtg aaaaatgcta tgctgatcga tcattgtact tgggatcaag
     4501 aatacaaaga aattacacaa tggataagaa aaaaattatt ggatcttgct caagtatcgg
     4561 aaggaatata cacaacggtc ttaatgcaag gtagcagctc cttcggggta gaatcggtat
     4621 taagttctgt gatcaggcca gaagatactg tactgatttg ttcaaacggc gcatatagac
     4681 aacgaatgat caacatgtgc gaatgtctga aactgaacta taccgtttat gaggtatcgg
     4741 aacatcaaat cctccaagct catgtgatta cacagctgat tgaagagaat ccgtctatct
     4801 ccgcactagc aatggtccat agcgagacta cttccggtat tttgaataat ttggaagaga
     4861 ttgctgctgt cacacaagaa agagatcttt tgtttatagt ggacgcgatg tcaagctttg
     4921 gtgggatacc gatttgtgta gccgaactag ggattgattt tttgatttcc agctcaaaca
     4981 aatgtattca aggtgttcca ggcttttctt ttgtacttgc taataaagcg gaactggaaa
     5041 aacgaaaaaa tgttagccga agtttgtcac ttgatctata tgaccagtac cgcgtgatgg
     5101 aaaacgagaa tggcaaatgg cgtttcactt cccctaccca tacggttttg gcttttcaac
     5161 aagcgctgaa ggaactagag gcagaaggag gaatcgaaac tcgatattca cgttatctcg
     5221 ccaataatca tgtgctacga caaaaaatga cagagatggg gttccagcct ttcattcaag
     5281 aatatcaggg gccattcatc acctcttttc tttaccctaa ggactggcga tttgatttta
     5341 atcattttta tcagtattta aagcaaaatg gatttgtgat ctatcccggg aaaatcagcg
     5401 atcatgaatg ttttcgtatc ggaaatattg gcgagatcta tccggaagat attttggaac
     5461 tgacagcaat tatcaaagac tataaggaga acgttaatga ttaaaactgt aatttttgat
     5521 tgggcaggaa caactgtgga ctttggctgt atggctcctg tccacgcttt tagaaatgct
     5581 tttctagaaa aaggaatcca actgactgac aaagaaatcc gctaaccgat gggaaaatta
     5641 aaatgggatc atatccaaca gcttctttcc ctcccttctg tcaaaaaaca atggatagct
     5701 atttatggac aactacctca ggaaaaagag gtggaggaat tgtatcaaca atttgaacat
     5761 tatctgttta aagatttggt caagcattct accttgaaac ccgacacatt ggaaacgatc
     5821 gaacggttga gaaacgctgg gatcaatatt ggttccacga atggatttaa tgctcctatg
     5881 atgacaatcg tagcagaaac cgcaaaaaaa gaaaggtatt caccagactt catggcaact
     5941 tttgaagacg tcgaagggta tggtcgaccg tttccttata tgatccataa aaacatgcag
     6001 tacttcaaaa acaagtcggt tgatgaagta atcaaagtcg gagatacggt ctcagatatc
     6061 caggaaggga aaaatgccgg agtgttgaca gtaggtgtaa ttgaaggtag ttcgctaatg
     6121 gggatttctt atgatgagta ccaaaaatta gagatgggga aaaagatcgt ttaaaacagt
     6181 ctgttgctct tagattccaa caagccggcg ctgatatttg tgtaaacaat ttaaaagagt
     6241 taacagaata cattttgtac gctgacaaga atccagaatg acttttatac aaaataagag
     6301 gttgtgaaaa agctgtcctg tatcaagtaa taaaaacagc caagcaaaaa tagctcctca
     6361 tatttttcgt ttggttaggc ttattaccgc agatgcaagc ttttgaacac cgtttattcg
     6421 aaaaaaaaaa gggagctgtg acacgacttt tgtcacagtc cccttatttg caaacaaaca
     6481 gtgtttaaga gacaacctct ccactaaacg gtgttcattc agctgactct cggtatttgc
     6541 tcaaaaaacg ataaaatttg aaaagctatt ttctcttttg tttctctaat actcggatca
     6601 gaacgctgat ttcacattct ctttttctta catcttatct ggtgcatgaa gtcctagcaa
     6661 acgaaggtct tctttcaata atacagtcac tgcataaact agagctaagc gtgcttcttt
     6721 ttgttcatca tcagctaaaa tcttcgtatg agcatagtat ttgttgaatg cttgtgctaa
     6781 tttgattgca tgtttcgcaa tcactgacgg ttcgtatttt tccccagcag acaagaccgt
     6841 ttcagggtat ttttgaacta atttaaccac ttcccagctg gtatcgtcat tcaatgcata
     6901 gtctgctgtt tctgaaggga caaatcctgc tttttccaac aggctgactg cacgagcatg
     6961 tgtatattgc acataaggtc cagtttctcc ttcaaatcga acaacttctt ccaggttaaa
     7021 gtcaaatgta ttcagtcgat cgtttttcaa atcgtggaaa atgactgccc caaccccaac
     7081 ttgtttagct acagcatctt tgttttccaa atcagggttt ttctcgctga tttgttcttt
     7141 agcagattgg attgcttcat ttaatacttc ttctaataag acgatctttc ctttacgagt
     7201 tgataatttt ttaccacctt gcgtgatcaa accaaatgga atatggtgca tgtcgtcaga
     7261 ccagtcaaag cccatttcct tcaagacagc tttcaactgt ttaaaatgat agctttgttc
     7321 attccctact acatatagag attgtttgaa gtcgtaagta cgtttacggt acaatgcagc
     7381 agctaagtca cgtgtaatgt atagtgtcgc accgtctgac ttcttaatca acgctggatt
     7441 caggtcataa gcagataggt caacgatctc tgcaccttta tcctcgttca gcaaatgttt
     7501 ttcttctaaa agtttgacga tttcatccat cttgtcattg tagaaagctt caccgttcaa
     7561 cgaatcgaaa cgtacttcca gcagatcata gattttattg aattctttca tcgattcatc
     7621 acggaaccat tgccataatt gaatagcctc tttatcgcct tcttccagac gtttgaacca
     7681 agcacgagct tcttcattta attctggttc tgtttcagct acttcatgga attggacgta
     7741 taaacgaagc aattcattga ttggttctgc tttaacagct tcttctgtac cccatttttt
     7801 gtaagcgacg atcagttttc caaactgtgt tccccagtca cctaaatggt tgatgcggat
     7861 tggctgataa ccgatttttt ccataatgaa accgatcgaa ttaccgataa ctgtcgaacg
     7921 caaatgtccc attgagattg gtttggcaat attaggagaa gacatgtcga ttggtactgt
     7981 tccttgattc ccgatgttgc tatccccata atgttctttt tctttcacta cggtttgcaa
     8041 aacttttttg ctgataagtt ctttattcat gaaaaagttc aagtaaggtc cgacaacttc
     8101 gattttttca aagttggcag aatcgatttt ttctgctaaa tctgcagcga tttgctgtgg
     8161 cgcttttcgg taaacttttg ctaatgaaaa ggctggaaat gcaacatctc cgtgttcagc
     8221 tgattttgga ttttctagta attgctcgac ttgttccagt gttaaatcat ctttgacagc
     8281 gtcatagaca gcttttgcga caagatcttt attattcatc tttattcctc ctaaagtgtt
     8341 cccttgattg ttaaacaaaa aaaatctcta gcgacggcat acttagtatg ccatcactag
     8401 agacgagatt tcccgcggta ccactccaat tgtcctaaaa aaggaccagc tccatctgat
     8461 atcggtagac gaccgtctgt ttaaaacaga attctccaag tgcgcttcgt tgtatcttct
     8521 tcattcggct tccaccactt ccgaattcgc tagccaagaa tgatacaagt actctcttgt
     8581 tcaacaaatc ttagtcttat taaactttct tgaattatat cagaaaaaaa aacgaaaggc
     8641 tagtcgtttc ataaaaaaaa aatccagatg gcctaaagga gaagaaatac cacctggata
     8701 gctataaatc atttcgtaaa tacgaaagac ttaacaaatt aatcagctac gataatcgtt
     8761 ccttcttcag aagctaaaag attctcgata ttttctaaag aagtgatgat tgccttagcg
     8821 tttggttttg cttcaacaaa tgcaatagct gcttctactt tcggaagcat gcttcctgga
     8881 gcgaattgct tttcatcaat gtattgtttc atttctgaaa cagtaacagt ttctaatttc
     8941 ttttgatctg gtttttggta attgacatac acattgtcta ctcctgtcaa tacgatcagc
     9001 aaatccgcat cgatgatttc ggctaatttt tctgaagcaa aatctttatc gattacggct
     9061 tctcgtcctt ctaatccagt agctgtttct acaacaggaa tcccaccgcc accaacagag
     9121 actgtgataa ctccttgatc gaccagcgtt tcgatgacac gtgcttcttt aatcgagatg
     9181 ggtttagggg aagcgactac ttttctccat ccacgtccag catcttcttt gaatgtcaca
     9241 gtggagtcag cattcatttg ttctttggct tcttcttccg tgtagaatgg accgactggt
     9301 ttacttggat ttttgaatga aggatcattt tcatccacaa taacttgagt gactaaagaa
     9361 accacatctt tatcgatccc tttttccttc agtacttcgc ccatggcgtt ttgtaaccag
     9421 taaccgatag atccttctgt cattgcaaca catgtatcta aaggcattgc cggtgttttc
     9481 tcagaatcag cagcttgctg ctgaatcaat aggttaccga cttgcggacc atttccgtgg
     9541 gagatgatca attcatctcc ttgttcgata aatttcacta aatactttgc tgtttccatt
     9601 aacgcttctt gttgggcttt tgcacttgca tccgtagaca agatggcatt gccccctaaa
     9661 gcgacaacta cttttcgttt ggccatttca ttcgtccttt cttcagttct accgaatttt
     9721 ctagataaca aaacgagcca gcgttttgtg attttccctc aatgctgact cgttttttcg
     9781 tgagatgaat gtggttatgc ttaaacgcga gggataaaca agttacctaa tgttgctgcc
     9841 atgatcgctt tgattgagtg catacgattt tctgcttggt caaattgacg agcatatttg
     9901 ctgcggaaga cttcatctgt tacttccatt tcagtgatgc caaagcgttc tttcatttgt
     9961 tcaccgtaaa cagtgtttgt atcatggaaa gctggcaagc agtgcaagaa gatcaaacga
    10021 tctgtgttgt gtgttttttc taccatttcc atattgattt ggtaaggttt caacaatttg
    10081 atacgttctt cgaatttatc ttcttcaccc attgataccc atacatcaga ataaaggacg
    10141 tctgctccgt ctacaccttt atcaacatcg tcggtaatca tcaattgtgc accggatttt
    10201 tcagcaaagc cttctgccat tttgacgatt tcttcttcag gttgtaattc ttttggtgca
    10261 acgatacgca tattcgctcc taaaattgca cctgtgacta ataaagagtt agccatattg
    10321 tttcgaccat ctccgcagta agcaacagtg ataccttcta ctgttccaaa gttttcttgg
    10381 atagttaaaa agtcagcgat catttgtgtt ggatgccatt catcagtcaa gccattccaa
    10441 actggaacac cagagaattc tgctagttcc tctaccattt tttgactgaa tccacggaat
    10501 tcgattccgt caaacatacg tcctagtact ttagctgtat cttctgtgga ttctttttta
    10561 cctaattgga tatcatttgc acctagatat tcaggatgtg cacctagatc gatcgctgca
    10621 gtagtaaatg cagaacgagt acgtgtagat gttttttcaa ataaaagcgc aatatttttt
    10681 ccttctagat agtgatgtgg gataccacgt tttttcaagt ctttcaagtg ttctgaaaaa
    10741 tcgatcaaat attgtaattc ttcttttgta aagtcttttt ctgctaataa gcttcttcct
    10801 tggaatactg attctttcat attaaacatt ctcctttaga ttgtttttat ttaagattaa
    10861 agaggaaatc ccgacggcag ctgacagtaa gactgccgtc aggaaacaaa ttacttcaag
    10921 tcttcacgga caagtggttg gctcatgcag cgtggaccac cacgacctcg tgacaattca
    10981 cttgaattga tttctaacac tttgatgcca tagctacgaa gcaattcgtt cgatacataa
    11041 tttcggttat aagtgactac aacacctgga gcgattgcca atgtgtttga tccatcattc
    11101 cattgttctc gaggtgcaac gatctcatct ccattaccag ttgggatcag tacaagatca
    11161 tctaatccaa gagcagcttt catcgtttcg tgtaagttat ctgaatgagt aatcttgata
    11221 tcatcaccat ctggttcgat tgtaaagaca tccatcttac cgtctttact ttgaattgct
    11281 ggatggatag tgaatttatc gtgatctacc attgtaaata cggtatccaa atgcatcatt
    11341 gcacggttgt tcggaatttt gattgccaac actttttcaa atcctgaatt ttttgcgaat
    11401 aggtggcgtg ctaatttttc taaagctttc gcattagttc gttgagaaat cccaacagct
    11461 actactttat cgcttaagat caattcgtct ccgccttcga tatgatctgg attttcacga
    11521 tccaaccata cttcgattcc tttatttgca aatcttggat gatgtttcaa aatatattct
    11581 gtaaacatcg attcacgacg acgtgcttca aaagtcatct tattgactgt catcccattt
    11641 cccattgatg cggaagggtc gcgtgtaaag tacaagtttg gcattgggtc catgtaaaat
    11701 ggatagtcat cgtctgctga cagatcatat agactgctag aacgtacttc gatatctttt
    11761 gtacgaaccc cagccatgat tttatctacc atttcttgcg tttccattga tagaaggaac
    11821 tcatgaagac cctctcttac agcattcgat gcaatatgag attcatccaa cattttattc
    11881 aagaattgtt ctttgacatt gcctgcgtca atcgcttctg ccgctaattt ttccaagtaa
    11941 agagtctcga cgccttcatt ttgcaatgtc ttcgcaaagt tgtcatgttc ttcttgagca
    12001 atcggaagat atggaatatc atcgaacaac agacgatcca taatatctgg tgtcaaattt
    12061 tccacttctt gtccaggccg tttcagtaaa actgtcttta atttccctat ttcagagaaa
    12121 acgtgaatag gtttatccat gttgtgttgc ctccttcttt ttgattacat gtataggata
    12181 tcctcctttt gtaatcactt tcataatttt gtctcattca aaatgagaca ttcctcacat
    12241 taatattcga taattaaata attaaacgtt tttctatttt taagcgtaat ttattcattt
    12301 tttattctgt atacactttc aaaaaaatat ttattcattg aaaaaatttt tttgaataaa
    12361 ttaaaaaaat aaattatatt atcgttgtta gcgtttacta tatgtattat tgttgttttt
    12421 tatacacaaa tcattcgctt caaaacaaat tattcaatac cattgtatat tttttacata
    12481 ataacaaatt atatttgtaa agtattaaaa cttgtttcta atgttggtat gttaaaactt
    12541 tacaaattct ttaaaaaaag tgacaataaa tatagtgaac aataaggaga ggacttttta
    12601 atgcgtgtta ataaaatcga ttttcacttt tcaaaactca gatatcaacc tgattttgaa
    12661 catttctcgg atgaagagtt ccaacagatc aaggaacata ctgttcttcg ttcttataaa
    12721 aaaggacaga tccttttcga cgatggtgat cccagacaac ggttgtatat cttgaccaaa
    12781 ggactcgttc gtttagaacg ttatgatgaa agtgcaactt attactacta tgactatgtg
    12841 aatacaaata acctttttcc tatgtcagga atgtttggag aagagaccta cacttataca
    12901 gctcaagcga tgactgatat tgaaacattc taccttcctg tttcctacta tgaaaaatta
    12961 gcacgaaaaa atcccgaaca gttgatctat atgatacaaa aaatttctaa agtagttgaa
    13021 atacatgaat tgcgtatcca aatcggattg acctctagcg cctttgaccg ggtaaaacaa
    13081 aatttattca ttttgaaaga agaattgggc gtaccaacaa aagatggtca tatcaccatt
    13141 ccttatccga tcaccttaaa agaattagca gtgaatagcg gcacgacacg tgaaactgct
    13201 ggacaagtaa tcaaacaact acgtgattcc gggctgttag attatgacaa aaagcgtttt
    13261 actttctgta agacagctac tactccagct atgagagaat aacgcgaaca aaaataagca
    13321 gtgaccctga taaat
//
LOCUS       DAFEOM010000072        13052 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.075, whole genome shotgun sequence.
ACCESSION   DAFEOM010000072
VERSION     DAFEOM010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.075"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(77..149)
                     /locus_tag="LP524_002263"
     tRNA            complement(77..149)
                     /locus_tag="LP524_002263"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="LP524_002264"
     CDS             530..1099
                     /locus_tag="LP524_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBL2235285.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="LP524_002265"
     CDS             1086..1946
                     /locus_tag="LP524_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBL2235286.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /locus_tag="LP524_002266"
     CDS             2136..2840
                     /locus_tag="LP524_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2235287.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="LP524_002267"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="LP524_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HBL2235288.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="LP524_002268"
     CDS             4677..5993
                     /locus_tag="LP524_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235289.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="LP524_002269"
     CDS             5994..6863
                     /locus_tag="LP524_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HBL2235290.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="LP524_002270"
     CDS             6918..7727
                     /locus_tag="LP524_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBL2235291.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="LP524_002271"
     CDS             complement(7830..8459)
                     /locus_tag="LP524_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBL2235292.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="LP524_002272"
     CDS             8629..9921
                     /locus_tag="LP524_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL2235293.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="LP524_002273"
     CDS             complement(9997..10659)
                     /locus_tag="LP524_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBL2235294.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /gene="groES"
                     /locus_tag="LP524_002274"
     CDS             10884..11168
                     /gene="groES"
                     /locus_tag="LP524_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBL2235295.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="LP524_002275"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="LP524_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBL2235296.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4339 a         2255 c         2824 g         3634 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12961 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    13021 tgtttagagc gtgagacaaa agtgttcttt ac
//
LOCUS       DAFEOM010000073        12598 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.076, whole genome shotgun sequence.
ACCESSION   DAFEOM010000073
VERSION     DAFEOM010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.076"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(11..160)
                     /locus_tag="LP524_002276"
     CDS             complement(11..160)
                     /locus_tag="LP524_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235297.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            556..756
                     /locus_tag="LP524_002277"
     CDS             556..756
                     /locus_tag="LP524_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL2235298.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            1324..1887
                     /locus_tag="LP524_002278"
     CDS             1324..1887
                     /locus_tag="LP524_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235299.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(1948..2292)
                     /locus_tag="LP524_002279"
     CDS             complement(1948..2292)
                     /locus_tag="LP524_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235300.1"
                     /translation="MISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFHYSPYRLF
                     QYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHDEVYLAEC
                     LANLEQKGIYLT"
     gene            complement(2389..2634)
                     /locus_tag="LP524_002280"
     CDS             complement(2389..2634)
                     /locus_tag="LP524_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2235301.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(2704..2958)
                     /locus_tag="LP524_002281"
     CDS             complement(2704..2958)
                     /locus_tag="LP524_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235302.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(3122..3445)
                     /locus_tag="LP524_002282"
     CDS             complement(3122..3445)
                     /locus_tag="LP524_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235303.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            3632..3705
                     /locus_tag="LP524_002283"
     tRNA            3632..3705
                     /locus_tag="LP524_002283"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3666..3668,aa:Ile,seq:cat)
     gene            complement(4090..4587)
                     /locus_tag="LP524_002284"
     CDS             complement(4090..4587)
                     /locus_tag="LP524_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL2235304.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(4627..5436)
                     /locus_tag="LP524_002285"
     CDS             complement(4627..5436)
                     /locus_tag="LP524_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL2235305.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIDMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            5770..6627
                     /locus_tag="LP524_002286"
     CDS             5770..6627
                     /locus_tag="LP524_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBL2235306.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            6620..7522
                     /locus_tag="LP524_002287"
     CDS             6620..7522
                     /locus_tag="LP524_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBL2235307.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            7555..7905
                     /locus_tag="LP524_002288"
     CDS             7555..7905
                     /locus_tag="LP524_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBL2235308.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            7954..8877
                     /locus_tag="LP524_002289"
     CDS             7954..8877
                     /locus_tag="LP524_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBL2235309.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            8955..9848
                     /locus_tag="LP524_002290"
     CDS             8955..9848
                     /locus_tag="LP524_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL2235310.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            9886..10449
                     /gene="amaP"
                     /locus_tag="LP524_002291"
     CDS             9886..10449
                     /gene="amaP"
                     /locus_tag="LP524_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBL2235311.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            10463..10651
                     /locus_tag="LP524_002292"
     CDS             10463..10651
                     /locus_tag="LP524_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBL2235312.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            10664..11566
                     /locus_tag="LP524_002293"
     CDS             10664..11566
                     /locus_tag="LP524_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBL2235313.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            11598..11840
                     /locus_tag="LP524_002294"
     CDS             11598..11840
                     /locus_tag="LP524_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBL2235314.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(12096..12467)
                     /locus_tag="LP524_002295"
     CDS             complement(12096..12467)
                     /locus_tag="LP524_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235315.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         4462 a         2113 c         2365 g         3658 t
ORIGIN      
        1 tttccactcc ttatgcagat ttttttaatt cttgcactgc ttgttcgatc gtaacacctg
       61 tcgctacatg attggtattt tctttgtcaa aaacgacaaa tagattcaaa tctgtatcca
      121 aggttactcg atattgtata tctttgttat agaatgtcat agccattctc ctttctttaa
      181 atacttgctg aaaggcaacg aacgcgttga taacgaaatt ccatttataa gtataccacc
      241 aattgaaagg ttttcatagt taagaaattt taaagtcaaa agttgcagat aaaagcaaat
      301 aaacaagaaa acaaaaagtg aatcaatcgc ggttattcaa ctttttcttc gattcgaaga
      361 caatagaaga caatgaattt tatatttcat tgcattatcc ttgtgttatt agaaaaagca
      421 tgatatatta aatgagtaat ctattttgaa acgtaatttg agcgatatat ctacatagat
      481 aaaactcctt ttgcctatca aatatttatt gcaacgaaaa aacgtacatt gatacgtatc
      541 aggaggaatt tatttatgaa taacggtaca gtaaaatggt tcaacgcaga caaaggtttt
      601 ggctttatca ctggtgaaga tggaaatgat gtatttgcac atttttcagc tatccaaggt
      661 gacggcttca aaactttaga agaaggccaa gcagtgtctt acgatgttga agatggtcaa
      721 cgtggccctc aagcaacaaa cattgttaaa gcttaataaa aattgacttt taaccgtctt
      781 ttcgaaagaa aagacggttt ttttgtacga aaaaattcat tggtcgttga atgggctgaa
      841 tagtagtgta taaaacaatt ttgtggagcg tgaagctttt atggtagagg aaaatacgaa
      901 taaagtgatg ttgataaaaa gattcctatc ttgaatatga atcgagatgt cttgaaaatg
      961 aatgagtttg accaatcatt atcctatcga aatttacagt ttcgttcgat tggtctgtca
     1021 attaatgaaa aagtaaaaaa gaatatattc taggaagagg gcggcacatc taaattttta
     1081 agtaacggac agttcaaata tcaagcattg aaagaggcta tcctactgtt tgaaggaata
     1141 cgaatatata aaaacgacaa agaggaaaaa gtagttcatt aaatcccttc aagtgatgaa
     1201 agaatgagat ctaataaaac ctgatctttc atctgttttt ttaataacgt aataattttt
     1261 ttattttttg tgctttcgcc gttattgtag agtgatttag cattcttatt tttaaaaata
     1321 actgtgttat atattaaaaa aggcataata gttataagct tttttaaaga atatttagaa
     1381 attattaatt ttcgtctgaa aacaaaaaaa gaaagcgtta ttattttagt ctattttcag
     1441 ggggggaacc ttatgataaa aaaactattt ttactatcaa tattaacgct agcaatggca
     1501 ggttgttctg actctggctt agcaactgaa ctgatcacaa aatcaactga agccacgaca
     1561 gaggagttaa aatgtggaaa cgatcacagt gactacagag gcttaaatcg acgatgcatg
     1621 ggaatattag gcagaagcaa ttgtaagcat cgatgtagta atcgaagaag gtgtagaaga
     1681 agcaacagaa aaatcaaaag aaagatcaaa agaagcaatc tgtgctgtgc atcgtataca
     1741 gacgtcgaag atgcggtact tttaaaccac gtcaggagat gttcatttat gtctagggaa
     1801 gttggcgatg aaccaaaact agagtacgct tttagttttg cacgtgagga tgaagataac
     1861 cctaaaactt acgacatttt agcttgatct aagatggtta aagcttttta atgaagtaag
     1921 aggagataga aggcttttct agttatatta ggtaagataa attccttttt gctccaaatt
     1981 tgctaaacat tctgctagat aaacttcatc gtgttccgga taaatcggca cgttgcgctc
     2041 tacagtaggt aaatcaaaaa atgcttcgca tgatttgcca cgatacatag gatttttcca
     2101 ctctggtgct gggctgtaac cacgtttttc catttcatcc atgacaagct gatgatattg
     2161 gaataatcta taaggagaat agtgaaaaac ataatccaca gtagcatgtt tctttcccca
     2221 gccatttcct cttaatgcac agcattcccg atgttgtcct aaaagctgtt gacgaggaag
     2281 ctttgaaatc aatttttcat gccataatca catagaaatg tctcctttat atatagattg
     2341 ttaaccttag acgttaaatg aaaaaaccag caccaaggct ggaaaaaatc acttcagttt
     2401 gcttgctgtt tctgagcgac caattaggta atcaacggaa atatcgtaaa agtcagcgat
     2461 atagattaat tcaatgatgc ttggaatttc ttctccttct tcgtacttac gatatttgaa
     2521 taaaggaata cttaacgcgc tagaaacagc tacttgagat aaagatcggc cagttcttaa
     2581 gaaattcaga cgacaacgta aagtatccag taaaatagct tcttcgatac tcatttaatc
     2641 acctttcatt atagtagaat agttataatg actatagaaa gctacacatg caatcaatag
     2701 tactcaatgt tttttattta aacgtatcaa ataaagaatg acgatcaata aagacaaaac
     2761 cagtcctgat aattcttcta taaaaacact atcgggttca aagtagttgt aattaaaaac
     2821 tttccacata aagtagatag ccggaagagt caaaatagct aaataaaacc gttctttgtt
     2881 ccaattcata agataaacaa tacttgcgac cagtgcgacc gcaggcaccc aaaacaaaac
     2941 tctcaccaac gtaatcatgt aacggactcc tcaacgattc atataataaa agataacatg
     3001 ttattgcata catcacaaat gaaatgctag gaaaataaaa gaagaataga tcaatgaaaa
     3061 atgaaaaagc agaacagttt tctgctgata agcaggaact gttccgctga atgtgtctta
     3121 tctatcgaga aaaaatcaat ggattgatat ctaagtaatt caatacatat aaaaagagga
     3181 cgatcgcatt cactaaaaag gaaagaatga tgatcccata gcgaatggaa acatatttaa
     3241 caaatacttt tgtttgaata cgggcatatc tttttccttt atagctgagc atcatgacaa
     3301 gaagcaaaag ggtaatcaca ccttgaaaaa tcattccaat aaacagaatt cctgaggttt
     3361 gataatttaa ttggagaaaa taagctgctg ccgcaactaa atcgaataaa atcgcaataa
     3421 atggcaaaat aatcactcct aacattgaga ataaataaaa gaataacatt ttttctaaaa
     3481 taagcaaata aaaattaaag taacaaaata gttatgacac aagtaaaaga aataagaata
     3541 acaatattat tttacttata ttttgaaact tgtgagagtt tgtcattcgt gctatagttg
     3601 aaaagtaaca aaaataaatt ttatttatga tgtaccttta gctcagttgg ttagagcaga
     3661 cggctcataa ccgtccggtc gtaggttcga gtcctacaag gtacataaga ggttgtgaaa
     3721 aagctgccct gcatcaagta ataagaacag ccaagtaaaa acagcttctt atgtttttac
     3781 ttggctgttc ttattactgc agatgcaagc ttttgaatac cgtttaagga agaggttgtg
     3841 aaatcagcgt tttgacctga gtattagctt aacaaacgga aaaatagttc ctcatatttt
     3901 gccgtttgtt aagctaatac cgagggtcag ctgattgaac accatttaag aaagaggtcg
     3961 tgagcctgcc gtttcactct gtgtcataaa aaagccaatc tactttgata gaaaaagcac
     4021 gtaggactaa aacagttctg cgtgcttttt ttattgtgtg ttaaatatcg taaatgaaga
     4081 tagatatttt tatgattcga cgatagagac gttgaaatct ttcaacagct ctaaagcgga
     4141 ttctccgtgt tttctactgg agactaacat tggatcaaca aaaaagtctg cttcaggaaa
     4201 agcactctgc aggcctatca gattagagag tacacacaag tttgtttcaa tcccgactac
     4261 ttcgatctct ttttgttctt ctttgctttc aaataaagtt tcctggattt cagctaaagt
     4321 ttcaggtgga atcgtaaagt aatacttttt aatcactcgt tcattttctt gtggagaaag
     4381 aagagggatc aattgcaaat cttctcgttc ttcttctttg tccttcagtt caactggtat
     4441 atcccgtgtg aagagaatat attcattatt ttggcgtgct ttagctaatc tttgagcaat
     4501 acgttctgcc aattgatctg actctggaat ataaaattcg ctgtttggat caagaatatg
     4561 attttgcata tcaataataa ctaacatttt ctttcctcct acattcatat cgtcatttta
     4621 ttctttttaa tagaagtttt ttaaaatata agcaacacca gcttcttcat tcgtcagtgt
     4681 tacttggttt gcaatttctt ttacttgtcg ctgtgcattc cccatcgcaa tactcaaccc
     4741 gctgtaattc aacatatcaa tgtcgttttc ttgatcgcca aaagccatga tgtcactcga
     4801 ttcgattcca aggtaatccg ccagaaaaac aagtgaatga gctttagtga tacctgtagg
     4861 cgtgatttct aagaaatgcg gatcagagaa tactgattgg ataccatctg cttgcagatc
     4921 tctttgtagc ttctgtagtt tttcttcact cttatctgtt aatgctaatt taagatagct
     4981 aagagaagga tcttccaaca tatttaaaat ttgatcaaaa gaatggttct gtaaattgaa
     5041 atgttcataa taagggtcat ctttgactag atttgattca tcgaatgtca gctgatcatc
     5101 ttgggtttct cggctagcaa tgatggatat tttcgcttct tgagcataga agaaggcttt
     5161 tctcagtgta tcgctatcta tttttgaacg tctgagaact ttcgccattt ttccatcctt
     5221 catttcagca atcagtgaac cattggaaga aatcaaatag ccaccgattc ctaattcgga
     5281 aaaaatatct ttagcagaaa tgctgttcct tccggtagcg acggttacga ttccgccatt
     5341 ttcatcaaat ttccgaagtg cttggatatt ttcttctggt atctttccat ttcgatcaag
     5401 caaggtaccg tctaaatcaa tcgctgctag tttcatttta tcttcctact ttctttttac
     5461 atatctattt tcctgttacc ttaagattaa atgagaattt aaaaaaatga aactaacttg
     5521 ctttgtcatt atgaattttt tcaaaaaaca ttcttaagaa aaaggtggct atatatagta
     5581 gtttttggtc aatacctttg aataaaaagc catgaaaaac gaatagttag cgcttaccga
     5641 tgcgttttgt gataaaaaaa ttttaaaata ttcaatgaaa acaaagcata tattttgctt
     5701 taaaaccaaa tgaggaatat tctagatgta gaacaggtta gaaaaagatt agaaaaagga
     5761 gtgattttga tggagaagag atacggcgta gttcctgtgg taactgcttc agacgaaaat
     5821 tatgctcctt acttgagcgt gatgatcgca actgcattag aaaattgcaa taaaacaaga
     5881 cgcataaaat tttatgtgat tgacgatggt ctatcagaat atagtaaaga aggattggaa
     5941 gaaacggtaa ataaatattc aagtaatgca agtatccaat tcctgacagt ggaaaaagat
     6001 atctacgaag acttcctagt cagtgaccat atcacaacta ccgcttactt aagaatttct
     6061 ttaccaaact tattagctaa agaggattat aagaaagtcc tttacttaga ttcagatgtg
     6121 ttagtactag acgatatcgt aaaactatat gatgagccgt taaatggaaa aacaatcggc
     6181 gctattatcg atccaggcca agtgaaagct ttggaaagat taggaatcga ttccgatgac
     6241 ttgtacttca attcaggagt gatggtgatt gacattgatc aatggaataa aaaagaaatt
     6301 acagaaaaaa ctatccatta cctcagtgaa aatggcgatc gaatcatcta tcacgaccaa
     6361 gatgctttga atgctgtact ttatgaagat tgggaacaac ttcatccaaa atggaacatg
     6421 cagacttcgc tgatttttga acgtcatcca gcaccaaatg aaaaatatga acgtcagtat
     6481 aaagaaggaa atgaaaaacc ttcgatcgtt cactttacag gacatgataa accttggaat
     6541 acgttgaaag atcatccgta taccaattta tacttaaaaa aattagcaca cagtactcta
     6601 acgaaagtag gcgagatcaa tgaataaaaa agaaatagca gtagtcgcaa gttgtaacac
     6661 aaaattcgta ccacatttag ctgcattatt tgtctcagta ttagataatt gcaacccgtc
     6721 taaatttgtt cggttctatg tgattgacga tgatatcgat tttgaaagta aacagctttt
     6781 acgcttctca gtgaaaaacg cacgaatgaa ctcagatgtg gaattcttga aaatcaataa
     6841 agaattcttt acaaatgtgg taatcagcga tcgtattcca gaaacagctt attatcgtat
     6901 tgcgattccg gaattgttcc gcggcacaga agttgaacgt atcttatata tggattgcga
     6961 catgatcgct ttacaagata tctctaaatt atggcgtctt gacttcggtg attcaatcgt
     7021 agcagctgtt gaagacgctg ggttccatca acgactggaa aaaatgaaaa tttcagctaa
     7081 atcaatgcgt tatttcaact caggtttgat gctgatcaat gtgaaaaaat ggttagatga
     7141 aaatattacg caaaaagtat tagatttcat cgaacataat ccagaaaaat tacgattcca
     7201 tgaccaagat gcattaaatg ctatcttaca tgaccgttgg ttaccacttc atccgcgttg
     7261 gaatgcacaa ggctatatca tggcaaaagc aaaaaaacat ccaacagcag caggtgaacg
     7321 tgaatacgaa gaaactcgca acaatcctta cattatccat ttttcaggac atgtgaaacc
     7381 gtggagcaag gattttgaag ggccaacgaa gaaatattac gaaaaatatg cagggatgac
     7441 ggctttccgt tgtgtagcta aattccctaa atatccaaaa tatgcaaaag ttcaaccaaa
     7501 aacagatgta gttgttaaat aatagaagac aacaactgaa gagggggagt aggaatggaa
     7561 attacaatgt ctaaaaatgc agtagagacg ttaattgaaa aggttgggga aaatacaaaa
     7621 atcgcactcg cgcttatcaa tgattccgat ccatttctaa gggacaaagg tgcttttgca
     7681 aaaggcagct tttttcaaat cattcctttt gtttcggaat ttggagaata tgcgactaaa
     7741 atcgagcatc cattattgga catctataca tctaaattag agcaaaatta tttcggtaaa
     7801 cgattgaaca tggacttcaa caaacagctt gacagctttt cattagaaaa tgaaatcgca
     7861 gtactcgatt ataatatcaa attaaagaac tgcttcttca gctaatagtt tcgagaaagt
     7921 ccaaaaagga ggtatacatt gagatactac tttgtggatg aagaaaagaa tgtaatagaa
     7981 acagatgaaa aaaaatataa ttggctcgta cttgatagtg caaagcccga agagattgag
     8041 caagcaatcg aagaattcga gcttcctgat gatattttcg taggaaccga atatccggaa
     8101 gaagtttctc ggatggagcg cctttatgag acgaaactag agcatccatt ttctttggta
     8161 gtgattaatc tagatcagtc agataatcgt atcgaaaaaa aattaacgcc aatatctttt
     8221 gtactttccg ataatctctt gattacttgt atcgatagaa aaaccgaatt tgtggatcgg
     8281 ctgctgacaa agcatggaaa tcatttaaac tcgtttgaaa agatcatcac gtatacatta
     8341 ttagatattt acacccatta cgtgaaagaa ctaagagaga tgaaaaagcg aattgacgct
     8401 ttggataaag cagctcgtaa aacaacggaa aatgaagaac tgtataaaca ggcagatctt
     8461 gaaagagaca tcgtatatat cgatcatacc ttaagagatc aacgggaaac aatggatcaa
     8521 ttatggaatt ctgaaacttt catcaaacgt ttagacgatg aacagctact ctatgatgtc
     8581 catttgagac agcgacaaac agaaaaaatg atcgagatct atcgagattt attagagagt
     8641 attggtgatc tattcaacgg gatgatggac aataatctaa atcatatcat gaaatatcta
     8701 gattcagctg cattaatcat ttccgttccg gcgttgttcg cagggatttg gggaatgaat
     8761 acaggaggat taccgggtaa aagttctgct ctaggcttta tactcgtagt ctttggctct
     8821 ttgatagcag cagtagtttt aggttaccat ttataccgga aagattatac aaattaaaga
     8881 atctatttaa aacaggtatc aaacaaagaa atttgttttg gcaatacaat ttaaaaacta
     8941 ggaggaatac agcaatgaca gaaccaaaat taatcgaccc gcgaaacttg taccatacgg
     9001 agaaatttcc taaacaagat caggatacac cagctttaca gcagaatatg attcctgttc
     9061 ctgattgtgg agaagaatcc tatgaaggca ataatcaact agaaaaccgc cgtgtactaa
     9121 tcactggtgg agattcaggg atcggtcgtg cagcagcgat tgcatttgct agagaagggg
     9181 cagatatcgc tcttcaattc ttcccaggag aagaaaaaga tgcagaggaa gtagcaaaat
     9241 acgtgaaagt tgcaggacgt aagattgtct tactaccttt tgatcttcgt gacgaacaag
     9301 cacctaaaga aatcatagat aaagccgtga gtgaacttgg cgggctagat acgatggtac
     9361 taaacgcagg acagcaaatt tcttgtcagt ctattgctga gttaccactt gagcaagtca
     9421 aagacacatt tatgataaac atcatcgcta tgtttgcttt agtgaaagaa gctgttccgc
     9481 atatcccagc aggaggagca atcgtaacaa caacttctgt tcaagcattc aatccaagcg
     9541 aacatttatt agattacgca gcaacaaaag cttcaatcgc taactttacg atcggtttag
     9601 caaaacagct tgcacctaaa ggaatccgtg taaatggtgt agcacctggt ccaatctgga
     9661 caccattaca actagacgaa ggacaacctt ctgattcgat ccctgaattt ggtcagcaaa
     9721 gtttactaga acgtgcaggt cagccaggag aattagcacc agtttatgtt ttcttagcat
     9781 cgaataaagc aagttatgtt acagcccaaa tttacggagt aactggtgga gaagcgatca
     9841 acttataaga agttgatcgt ttcaccccta tgaggaggtg cgaaaatgaa taaaggtgta
     9901 aaaacgatcg gagtgatcgg aacattactt ttgctatctg tgcttttgtt tgcgatacta
     9961 ttaagtgcaa cctatccatt gccttttcag ctagagaagt ttcgattctt ttccatcact
    10021 aattcatacg tgcaacaata tgtattttgg cttgccgttg catttgctat tttgacaatt
    10081 atcacgttac ttgttttcct gttttatcca aaaacaatcg gaaactttct actgaaacat
    10141 ggtgatgggg agttgacgtt ggataaaaaa gcaattgaag gaatggttcg ttctcacttg
    10201 catgaagatg aattcatcca ttcaccaaaa gtaaatgtca aagcaacgaa aaacaagatc
    10261 gccgtcaatg taaaagggga gttgaaacga acttcagcac tcattggtaa aacaggaaca
    10321 ttgatgaaag aaatcgaaaa tgaagtacaa cacatattgg gaacaaaaga gccagtgaaa
    10381 gtatcggtga aatacacagg ctttgaagaa ccagctaaac gaagcaatga ccgcaaacga
    10441 gtagaatagg gggggaccaa aaatgaaaga actattttcg acatacaagc tccctatcat
    10501 ctttggggta cttggattga tattagccgt tttatggatc accgtgggct ttttcaagac
    10561 cttattgatc ttgatcatgg cagcgatcgg cgtcgctatc ggtttttatc tgaaacaaac
    10621 gaaaatctta gatcactatt ttaaatctta ggaggaaaca tccatgaata acaataacaa
    10681 aaataataac aataaaatga acaaccaaaa accagtgtct gcacatccag acccaacgac
    10741 accaaaccaa tcaaatggtg tgaacgatga aaagaacaaa acagcaccag tatctgcaca
    10801 tccagatcca gtagtcccta acgttcaaca aggagtagaa gagaagaaac atgatgcaga
    10861 aaagcatgca cctcatcatc caggaactcc agaatctaaa gttccagaac aacatgatgc
    10921 aatcgatcct tctaaacctg tggaagagca aaaacatcct tctgaccatg cgaaagcaca
    10981 agaaaaactt agttctgacc acacaaacgg acaaggaaaa cttggttctg atcatacaaa
    11041 taaccaagga aagcatgatt ctgaacagga agattcaatg cgcggtgagc tgaattatga
    11101 agataaagtt gtgcaaaaaa tcatcggaat cgcaattgaa caagtagacg gtttattatc
    11161 tgcaaacggt ggtttcttct ctaatgtggc aggaaaactt gtgaatacag ataatgtaac
    11221 ggctgggatc gaaacagaag tcggtaaaaa acaagtagca gttgatatgg atgtcattgt
    11281 ggaatatggc aaagatatcg aaaaaatctt tgaagaaatg caagaagtaa tcggcaaaga
    11341 agtaaaaaat atgacacatt tagaagtgat cgaagtcaac gctaacgtag tggacatcaa
    11401 aacaaaagaa gaatttgaaa aagatcaaga aacggtgcaa gacaaagtaa ctgacgcagc
    11461 gaaaaaaacc ggtgaatttg cttcgaatca aacagataaa gtaaaatcag cagctggaag
    11521 cggcgcacaa aaagtaaaag aaaatacaga accacgggtt cagtaataag gaagtaaaac
    11581 attaggagga acgaaacatg ggatttattt ggtcacttat cgtcggagga attatcggag
    11641 caattgcagg agcaatcacg aataaaggtt cttcgatggg gattattgca aatgtcgtag
    11701 caggtttagt tggatcagca cttggtcaag cattattagg ttcttgggga ccaagtattg
    11761 caggaatggc aatcgtgcca tcattgattg gtgccatcat tttagtacta atcgtatcat
    11821 tctttgctcg aaaactgtaa aaagactttt aaaaagaggt cgtgaaaaag ctgttcttca
    11881 tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcattt ggttgggcat
    11941 attatcgaag atgcaagctt ttgaacactg tttatacgga ataagaggct gtgacaagac
    12001 ttttgtcaca gcctcttatt tatgcgggca aattcccaat tagattttta aaagtttttc
    12061 cttaaatgtg tctttattga tacagagaag cttctttaat aaaacaatgc ctccctaaat
    12121 tttcttgaga taggaaaggt gaacatacaa aataatacga ccactttgaa aacccagatg
    12181 atccacaaat ggatcgtatg atactgatga agtaaaaagc aatggatcag aaactcaaag
    12241 agattgaaaa agacaaggaa gaaataatat ctctttttta ctcttgtctg ttttagccaa
    12301 taatagctcg taaagtaatc tagacaaaca aatagaagta aacaaagttt cgtaagagca
    12361 gcaatcgtat catgttgctc tgctagacga ttgacgagca ggaaaaatgt tggtaatgga
    12421 atatagatca cgagtaatga aagaaaaaag taaacaataa atggcatact attcctccta
    12481 attgtgtgag ttcgccctca ttttaaaata acgatatttg tttgtgaagc tttttcgtgt
    12541 cgtaacaaat gttatctaat gtatttttaa ataaataatc attattatca aaataatg
//
LOCUS       DAFEOM010000074        12583 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.077, whole genome shotgun sequence.
ACCESSION   DAFEOM010000074
VERSION     DAFEOM010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.077"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            46..897
                     /locus_tag="LP524_002296"
     CDS             46..897
                     /locus_tag="LP524_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL2235316.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            951..2702
                     /locus_tag="LP524_002297"
     CDS             951..2702
                     /locus_tag="LP524_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL2235317.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            2706..2876
                     /locus_tag="LP524_002298"
     CDS             2706..2876
                     /locus_tag="LP524_002298"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL2235318.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(2916..3818)
                     /locus_tag="LP524_002299"
     CDS             complement(2916..3818)
                     /locus_tag="LP524_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL2235319.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            4049..5452
                     /locus_tag="LP524_002300"
     CDS             4049..5452
                     /locus_tag="LP524_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBL2235320.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            5452..6885
                     /locus_tag="LP524_002301"
     CDS             5452..6885
                     /locus_tag="LP524_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBL2235321.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            7030..7956
                     /gene="trxB"
                     /locus_tag="LP524_002302"
     CDS             7030..7956
                     /gene="trxB"
                     /locus_tag="LP524_002302"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBL2235322.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            8170..9897
                     /locus_tag="LP524_002303"
     CDS             8170..9897
                     /locus_tag="LP524_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBL2235323.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            9912..10262
                     /locus_tag="LP524_002304"
     CDS             9912..10262
                     /locus_tag="LP524_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBL2235324.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(10354..11214)
                     /locus_tag="LP524_002305"
     CDS             complement(10354..11214)
                     /locus_tag="LP524_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBL2235325.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            11306..11734
                     /locus_tag="LP524_002306"
     CDS             11306..11734
                     /locus_tag="LP524_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBL2235326.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            11896..12072
                     /locus_tag="LP524_002307"
     CDS             11896..12072
                     /locus_tag="LP524_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBL2235327.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            12099..12545
                     /locus_tag="LP524_002308"
     CDS             12099..12545
                     /locus_tag="LP524_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBL2235328.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         4132 a         2147 c         2774 g         3530 t
ORIGIN      
        1 ttttgttttg gatacagccc tatacatcat ggaaaggagc aaaacatgct gattgagaaa
       61 gtactgaata acaacgtcgt aataaccagg gatgaaagaa atcaggaaat gatcgtgatg
      121 ggaaaaggtt tggcattcaa aaagaaagtg ggtgacccga tttttgactc atgcatcgac
      181 aaaaaatttc agttgacaaa tgacgggctt tcacaacagt tccaagaatt ggcacaagcg
      241 attcctcttg agtacttaga aatttcttgt gaaatcattg aatatacaaa agaggctcta
      301 caagtccagt tgaatgattc tgtgtatatt gcattgacgg atcatttgta tacggctatc
      361 gaacgtcaca aaatgggaat atttgtacca aatgtattgc tgtatgatat caaacaacta
      421 tttcctaaag agtatgccat cgggaaaaaa acagtgaaga aaatctcgga taaataccac
      481 atcaagttat cagaagatga agccggattt atcgcattgc atttagtcaa tgcacaaggc
      541 attcaaagcc agcaagacaa tacctacctg tttactataa caatacagga tattctaaat
      601 atcgtccgct attactataa gattacattt gatgaagatt cagtccattt ttttcgattc
      661 gtcacacact tacgtttttt tctgacacgc gtcctagatg gagaaaaatc agaagtggga
      721 gaagtttctg aggaattgtt tcaggttatt caaaaaaaat ataaggatac gtttggttgc
      781 gttgagaaaa tagcagactt tttagccgag acgttcaact accagatgtc cacggacgaa
      841 aaaatgtatt taacgatcca tactgcgcgt ttagtgcatg agaatcgttg taaataaaaa
      901 aaggaggata aattaacaag aacgtatgaa ataagggtag tgacattcag ttggctaaac
      961 cttccactaa taaattggag ggaatagaaa tgggaaaata ccaagaatta gcgagaaaaa
     1021 tcgtagaaaa tgtcggcggg aaggaaaata tcaatggttt gacacattgt atcacaaggc
     1081 ttcgtttcaa attaaaaaac gaagaaaaag caaatgatga gatcttgaaa aatatggacg
     1141 gtattgtaac ggtaatgcga gcgggagggc agtatcaagt tgtcattggg aatcatgtgc
     1201 cagtcgtgtt tgaagaggtt ataaaagcag gaaaccttac ttttgatgag gctgtttcaa
     1261 cagaaaaaat gagaccgttt gatatgctta ttgatggaat atcgggctgt ttccagccat
     1321 tccttgctat cctagctgct ggcgggatga ttcgtggact gacagcattc cttgtcttct
     1381 taggcgcgtt tgaccgcggt tctggcacat ttgttatgtt tgataatatc ggtgattctg
     1441 tattccaatt tatgcctgtt attatcggtt taacagctgc ccgtaaattt aaagtaaatg
     1501 aattcgtagg gatgttgctt ggcgcagcat taatgaatcc tagcttgtcg ctggaagctt
     1561 tgagtggtgc agcagaagca ccactaacga ccatattttc tggcactatt tttgaagcac
     1621 ctatttatca aacagtattc ggcattccct ggattgcgcg taactacgct tcttctgtca
     1681 ttcccattat ctttattgtg ttgttagcta gccaaattca gaaaccaatt aaaaagttag
     1741 tacctgagat gatcgcgaat ttcttcgttc cattcttcac cgttcttatt acaatgcctt
     1801 taggtttttt actcgttgga ccagtattca cattcgcaac tgatatttta atggctggat
     1861 ttgaaacact tttagcactt tcgccagtta tttatggagc aatcgtaggc ttcttctggc
     1921 aaattctggt gatgtttggt ctccattggg caatcgtacc aatgggattg atgcaatttt
     1981 ctgtgaatgg ttggcaaaat attatgacac ctgttgcagt agtgtctttt ggacaaactg
     2041 cagcacttac cgcactctac tttaagctta gaaatccaaa agacaaagca attgcaatac
     2101 cagccattgt ttctggtatt gtggggatta cagagccagc tatttatggg tttacattac
     2161 cacgtaaaaa aatatttatt tttacctgtg tcggtggtgc gttagggggc gcgtatagtg
     2221 ggttaatgaa tttaacttct tggaatcaag gtggactagg catctttaca attcccaact
     2281 atattagacc agatggtgat ttaacagatg tgattaatgt attgattggg atcgccattg
     2341 cgatggtggt atcctttacg ctgacttttt tcttttggaa ggatgaagca ggtgaaacag
     2401 atgatataca gaaaaaatca ggtaaagaaa ttgtaaaaac accgatacaa ggacaaatcg
     2461 ctccactaaa tgcagcaaag gatgcagctt ttgcacaagg aacactaggt cgaggaatct
     2521 tgatctaccc tgaaaaagga gaagtacgtg ccccttttga cggaacagtc atgaccctgt
     2581 tcccgacgaa acaggcaatt ggaatggttt ctgaaacagg gctggaattg ctgattcatg
     2641 tcggattgga tactgtgcag ttggaaggga aatattttga aagcttagtg cagcaaggat
     2701 aaagagtgaa acaaggcgat ctgttgctac gcttcgacaa aaagaaaatc gaaaaggaag
     2761 gatacagttt agaaacacct gtgatcgtca ccaattacac ggattatttg gatattttgg
     2821 aaaaccaagg ggagacggtc gggccagacg atgcacttat caccgcactg acctgaacgg
     2881 ttaggtgaag aatggactag gcttaactat tctttttatt ccacttcttt attttcaaaa
     2941 ttgaaaggct ctttcatgat ggctgagctg atagggcgtt tatcgaattt ttgaccgaca
     3001 gaaattagat ggactaaatt ctgttgataa ttttctgcaa aaacacaact atataacacg
     3061 tctagaagat aacaaattga tgtattgatc gtaaaattac caatttttga atacagtcgc
     3121 tcgcgtgtcg taagtcgcaa agcggctgta ctcattgcag atagcgttga atcaccgata
     3181 ctggtcaaag ctaaaattgg aacatgattt tgtttcaata tttctgcggt acgaaggatc
     3241 acattgtttt cacccgtata agaaatcaga atcccgcaca tatcactcgt gcaattgtac
     3301 gcttcataga atgattcacc aaaagtggtg acaatcgtga catccatttt gatgcgcttc
     3361 attttcagca tgaaatcttg agaaatcaaa gtattcgagt tactggcgaa aatcacgatt
     3421 ttttttgctt gaagcattag ttgtttggct ttttgcagtt ggtcatgatg aatgagagaa
     3481 agtgtatcat gaatcgtcat ctgttctaaa gaagccattt tatttgcgat cgtcataata
     3541 ccgtctgtgg tggaaaaagg aagattcgca tctacgtctt gaaaattccc ttcaagatag
     3601 gctgtttcag ctaaaaaagc attacgaaaa ctgacccagc catcaaaacc catttttttt
     3661 gctacacgga tgagtgtaga ggggtggaca taacatcgct cagcaatcgc ttggatggtc
     3721 atttcgccaa gtgctggaga atcgcttaag agaaactgga tcacttcatt ttctgaagga
     3781 gaaaaggaga tttctttgat tttttcacgt aataacatgt agataccttc ttcttatttt
     3841 tttacgtaaa ttataacttt tttgaaaatg atttgtaaac ggatttatac tatataactt
     3901 aaaatagaca ttttttgaca agatatttac aaaaatccac tagaaaacag taaattttgt
     3961 aaatgttact ggaaagaaac agatttctat tgtttgtttt gtttacaaaa aagataatga
     4021 aagtgtaaaa aagtaaagga gtgagaacat ggcaaaagga atcaaaatcg ctacgattgg
     4081 tggaggcagc agttacaccc ccgagttgat ggaaggattt atcaaaagat atgaggaatt
     4141 accgatacga gaaatctggc tcgtagatgt agaagaagga aaagaaaagc ttgagatcgt
     4201 tagtcagatg tctcagcgta tgtgggatgc ttctccgtat gaagtcaaga tctatgcaac
     4261 tctggatcgg gaagaagcat tgaaagatgc ggattttgtt acgactcagt ttcgtgtcgg
     4321 tcaattagat gcacgaatca aagacgagcg gatccctagt tattatggga tgattgggca
     4381 agaaacaaat ggtgctggcg ggatattcaa agcttttcgt accgttccag tcgttttggc
     4441 aatcgttgag gacatgaagc gtttatgtcc aaatgcttgg ctgatcaatt tcgcgaaccc
     4501 aagtgggatg gtgacagaag cagttatcag atatggcaaa tgggataaag tgattggatt
     4561 atgcaatgtg ccagtgatgg cgatgatgac agaaccagca atgcttggca agaaaaaaga
     4621 agagctgatc tacaagtttg ccggtctaaa tcatttccat tggcacaaag tagcagataa
     4681 taaaggaaat gaccgcacgg cagaattgat tgatcgttta tacgatgaag agaatggatt
     4741 accgaaaaat atctttgata tcccgttttt caaagaacaa ttaaaacaaa tgaacatgat
     4801 cccttgtggc taccaccgct attactatcg tgaagaagaa atgctggaac atgctttaga
     4861 agaatatcaa acgattggta caagagcgca gcaagtgaaa gaaacagaag cagagctatt
     4921 tgaattatac aaagatccag aattagacca taaacctgaa caattgcaaa aacgaggtgg
     4981 cgcttattat tcagacgctg cctgtgaaac gattgcttca atctatggaa ataaagaaac
     5041 acagatcgtg gtatctacaa agaatgatgg agctgttcct gacctgccgg cagattgtgt
     5101 agtagaagtc actgcctatg ttggcgcaca aggagcgaga aatgtagcgt ttggttctct
     5161 tcctgctgct gaaagaggct ggttgcaagt aatgaagaat atggaattgc tgacgatcga
     5221 agcagctgtg actggtgatt atgggacagc tttacaggcg tttacgatca atccacttat
     5281 acgatcagga aagacagcaa aacggatcat ggatgagttg tttattgcac accagcagta
     5341 tttaccgcaa ttcaaggaga caatcagccg attaaaagaa gaaggaatcg aagttcaaga
     5401 tgaactggca agagaattat caggtaatac acatataatg gaggaagtat aatgggattc
     5461 agaaaagatt ttttatgggg cggtgctaca gcagccaatc aatgtgaggg cggttatgat
     5521 gaaggtggac gcggactagc aaacgtagat gttgtgccaa tcggaaaaga ccgttttcct
     5581 gttatcaccg gaaagaaaaa aatgtttgaa tttgatgagg atcattttta tccagcaaaa
     5641 attgcaatcg atatgtatca tcgttataaa gaagatattg ctttgtttgc agaaatgggg
     5701 ttcaagactt atcgaatgtc catcgcctgg agtcgtattt ttccaatggg ggatgagaaa
     5761 gagccgaatg aagctggctt gaaattttat gaggatgcct ttaaagaatg ccaaaagcac
     5821 ggaattgagc cacttgtgac gattacgcat tttgattgtc caatgcattt gatcaaagaa
     5881 tatggtgcgt ggcgttccag agaactcgtt ggtttttatg aaaatctgtg tcgagtcatc
     5941 ttcaatcgct ataaaggact tgtcaaatat tggctgacat tcaatgagat caatatgatc
     6001 cttcatgcac cattcatggg cgcagggctg tattttgaaa aaggagaaaa tgaagaacaa
     6061 atcaaatatc aagcggcaca tcatgagttg gttgcaagtg ccatcgcaac gaagattgct
     6121 catgaagtag atccggagaa caaagtaggc tgtatgctgg cagcaggagc atattatcca
     6181 tacacttgca atcctgaaga tgtttgggct tctagaaaat cagatcgaga aaattatttc
     6241 ttcattgatg tccagtcacg aggagaatat cccgcttatg cattgaaaga aatggagcga
     6301 aatggcattc agatcgaaat ggaaccaggg gatgccgcat tgctgaaaga acatactgtt
     6361 gattttatct ctttttctta ttatgcttcc cgagtttcga ctaccgacaa cgaactcttg
     6421 gaacagactg caggaaatat ctttgcttct gtcaaaaatc catatctgca agcaagtgaa
     6481 tgggggtggc aaattgatcc attaggcttg agaataacga tgaatgatat ttatgatcgt
     6541 tatcaaaaac cattgtttat tgtagaaaac ggcttaggtg cagtcgatac gccagatgaa
     6601 aatggttatg tggaagatga ttaccgaatc gattacctgg cagcgcatat ccaggcgatg
     6661 aaggatgcag tagaactaga cggagtcgat ctattaggct atacgacttg gggatgtatt
     6721 gatttagtat cagctggtac cggtgagatg aagaaaaggt acggattcat ttatgtcgat
     6781 cgtgataacc aaggaaatgg cacattagcc cgttcaaaga aaaaatcatt caactggtat
     6841 aaaaaagtaa ttgattcaaa tggagaaaat ctttcgaacg attgatcaat gatttataaa
     6901 agtctattgt ttttcagcgg atttttcgag tataataaag ctattcgaga aacgcaagca
     6961 gaacaataga ctttttttcg cattggctgt aaagtatgct tacttgatta agtaaaggag
     7021 aatctacata tgtatgatgt aatcgttatt ggcgcaggtc ctgctgggat gactgctgct
     7081 ttatatgctt cacgttcaaa cttatccgta ttgatgatcg aacaaggtgc ccctggcggc
     7141 caaatgaaca atacggcaga aatcgaaaat tatcctggat tcgattcgat catgggcccc
     7201 gatctagctt ataagatgta tgaaagtgtc tcaagatttg ggacagaaaa tgcttatgga
     7261 atcgttcaag atatcaagga tcacggcagc cataaagaag tcatctgtga agataaaagt
     7321 tacgaaggaa aaacagtcat tatcgcaaca ggatgtatcc accgtaaatt aggcgtacct
     7381 ggcgaggaag aatttgctgg acgaggcgtt tcttactgtg cagtctgtga tggtgcgttt
     7441 ttccgtaaca aacgtctagt agtcatcggg ggtggggact cagcagtaga agaagccatc
     7501 tatttgacac aatttgcttc tgaagtagtg atcgtccacc gtagagatga attacgtgca
     7561 caaaagatca tccaagaccg tgcatttgca aatgaaaaaa tttcctttat gtgggataca
     7621 gtcgttgacg aaatcgttgg aaacgaaatg gtagtgacag gtgtgaaagc acgtaatgtc
     7681 aaaaccggca atgaatttga tttagcagca gacggagtat tcatctatgt tggactggat
     7741 ccattaactg caccttttag agaagcaggt ttgacgaacg aagctggctg gattccaaca
     7801 gacaacgaaa tgaaaacgaa tatgccaggc gtttttgcca tcggagatgt tcgtgaaaaa
     7861 gatttgcgac aaatcactac agctgttggt gacggcggta ttgctggaca acaagtgtat
     7921 aaatacttgg agtctttaga agaaacaaca gcataagttt aaaaagagta gaagaacggg
     7981 ttcaattgtt cttttactct tttttttcgc gtgaaattta ctgaatctga tttatgcgaa
     8041 cacgtccaac accttttgat aaaagaatcc tttcttgatt taagcgtttt ttggtcctca
     8101 tactttcata tctaataagt gaatgctata cttattaaga accatatttc agaaagaggt
     8161 gttattttca tgtcttggga acaagtgtat gaacaatggg tacatgaaga aaatttagaa
     8221 gaaaacttaa aaaaacaatt aaatgatctg ggtgaagatc ctgaaaaact tgaagatgct
     8281 ttttatgcac cgttggaatt cggtactgca ggtatgcggg gaatcttagg acctgggatc
     8341 aatcgaatga acatctatac ggtaagacaa gcaacagaag gactagcacg tttcatggat
     8401 acacaaggtg aagaaacaaa acgccgaggt gtcgctatcg cttatgactc acgccatcag
     8461 tcaccagaat ttgcaatgga agcagccaaa acactagcac atcacaatat cccgtcttat
     8521 gtattcgaaa gtctgcgtcc aacaccagag ctttcttttg cagttcgtca tttgaagacg
     8581 tttgcgggaa tcatggtaac cgcatctcac aacccagcgg cttacaatgg atataaagtc
     8641 tacggagaag atggcggaca aatgcctcct gcagacgcag atgcattgac gaaatatgtc
     8701 cgtgaagtca gcaatccgct aaaagtcgaa gtattatctg acgaagaagc taaacatagc
     8761 gggttgatca cgatcatcgg agaagaagtg gacgctgctt atctagaaga aatcaaagca
     8821 gtgacgattg atcgtgaatt ggttgaaaca atggggaaag atttgaaact tgtgtacacg
     8881 ccacttcatg gaacagggaa aatgttgggc gaacgtgcgt tgaaacaagc tggattcgaa
     8941 caattcgtac tagttcccga acaagctgtg gcagatccag atttcactac ggtcaaatcg
     9001 ccaaatccag aagaacattc agcttttgaa tatgctatac gacttggaga aaaagaagga
     9061 gctgacttgc tgattgcaac agatcctgat gcagaccgtt taggcgcagc ggttcgtttg
     9121 ccagatggtt cttatgaagt attgactggg aaccaaatcg gagcattgtt tgctcaatat
     9181 attttggaag cacatcaatc aagaggcact ttaccagaaa acgcagcggt attgaaatca
     9241 atcgtttcca gcgaattgcc tacagcaatt gcggaacact acggtgctac catgttcaat
     9301 gtgttgacag ggtttaaatt cattgctgaa aaaatccaac aatttgaaga agaccattca
     9361 catactttta tgtttggatt cgaagaaagt tatggttatt tagttaaacc ttttgttcgc
     9421 gataaagacg cgatccaagc attgctttta ttagcagaag tagcagctta ctataaaaaa
     9481 caaggcaaaa cgttgtatga cggcttgcaa acgatttttg aaacctatgg ctatttcgca
     9541 gaaaaaacga tttctgtgac aatgagcgga caagaaggtt ctgcgaaaat ctctgctttg
     9601 atggaaaaat tccgtaaaga agcaccgaca gaatttgccg gagcaaaagt tattcaaaca
     9661 gaagacttta aagaattgac gagaacagac cgtgaaggaa acgtagaaaa actaacaaca
     9721 ccgccatccg acgtattgaa gtatacattg gaagaaaatg gctggatcgc tatccgccct
     9781 tcaggaactg agccgaaaat caagttttat attggtgtaa aaggaagctc tacagaagac
     9841 gcagccgaaa aagtagcctc ttatgaagcg attatcaaaa agatgacaga agaataaaaa
     9901 acttgagact gatggaaaaa gtggtaaact ttagaagtca gtcttttttc aagttggagg
     9961 tgaatggatt gccagacacg caaaaatcaa aagtagagaa agtcagtcaa ttattcaaaa
    10021 tgttgagtga tcccactcgt cttaaaattc tgctatatct gaaagacggg gaacaaaatg
    10081 tgacagcaat cacccaagca gtggaaatgg agcagtctgc tgtatcgcat caactacgtt
    10141 tattaagaga aaatcatgtc gtcaagtctc atcgcgaggg aaaagcgatt ttgtatagtc
    10201 ttgatgattc tcatgtactg gatattttga accagacact caaacatgtg gaacatcaat
    10261 aacgataggt gaaaatagat aaacactgga gtgtgacaac gaagaaattt cgttgtcaca
    10321 ctccagtgtt ttttaagaaa aagacaaaaa tgattaatcg cttttttctg ttgtttcagt
    10381 tccataataa ctatgatctt ctaggtctaa agcattcaaa ataagcgaag ccgtttcttc
    10441 aatagataaa gatgccacat tgatcactac acatcctagt ttttcataaa gatcattcgc
    10501 aaaatcgagt tcagctctaa ttttttcgat atcggaataa gcagtatcag ggtttaatcc
    10561 gtaagagcgc atccgttctt tacggatatt atttaaaata tctgggttat tcgttagtcc
    10621 cacgattttt ttaggatcga tttcccacag ctgtttggga atatgtgctt ggggaatcaa
    10681 aggtaaatta gcaactttta aatttttatt tgctaaaaac aagctcaaag gcgttttgga
    10741 agttcgggat acgcctagca aaacgacatc tgcttctaaa aaaccacgag gatctttccc
    10801 atcatcgtat tttacagcaa attccattgc tttgatccgt ttgaaatagt tctcattcaa
    10861 taaatggagg gcaccaggtt gccgaattgg cttgatacca gttaaacgtt caatttctga
    10921 tatcggtggc gtcatcacat cgaaactaaa taattgatgt tcttcacaaa aagaacgtgt
    10981 tatctcgatc agttcttgat tgattaatgt atgcaggaca agtgcttttt cttctaatgc
    11041 atcttgcaat gcctgcctta atgttttttc atctgtcacg aacgttctgc ggaataggtt
    11101 gaactcaacg gtaggatact gagccatagt agcttgcgct agttttgtcg cagtttctcc
    11161 agcagagtcg gagatgacaa aaaaagtaac gatttctgta gaatcggaag acatggataa
    11221 aacctcactt gcattttttt tatgtttatt ataccatact ctagaatgcg tacttcgtaa
    11281 tcattttgat ttggaggaat aagtcatgaa aaaacagaca ttgatcgaac aatcattaga
    11341 aacattgaaa aataacggtt ttaaatatac taaaaaaaga gaagcattgt taacttattt
    11401 agtgaagcgt aaccgctacg tatctgctaa agaagttttt gactatatga atcaggaatt
    11461 caaaggggta agttatgata cgatttaccg gaatctccat gattttgcag agatcgattt
    11521 actggaagaa accgatttaa atggtgaaat gaagtttcgt tttcattgct gtcaggggga
    11581 aatcggccat caccatcatt ttatctgtac agtgtgcgga acaacgaaag aaatccatat
    11641 gtgtccaatg aattatttcg aagaacagct aggtggatgc acgatcgaag ggcatagatt
    11701 cgagattttc ggaagatgtg aaaattgtca ataaggattt aaataaaata acatttgata
    11761 atttaaaaaa aaagcaagat ttccttgacg gtcaaaacag cattagatat aatatttaca
    11821 ggacagtctt gttttttgag atatatttca ataacaatta cggttacttt ttaagctcgg
    11881 agggagggaa ttaacatgtc aaaaacagtc gttcgcaaaa acgaatcact tgacgatgct
    11941 cttcgtcgct tcaaacgttc cgtgtcaaaa gcgggtactt tacaagaatc tcgtaaacgt
    12001 gaattctacg aaaaaccaag tgtaagacgc aagaaaaaat ctgaagcagc tagaaaacgc
    12061 aaaaaattct agttctgtga tgaattggag ttggtagtgt gtcactacta agtagattaa
    12121 acgatgacat gaaagcagcg atgaaagcga aagataagga atctttacaa gtcatccgaa
    12181 tgatcaagtc atctatacaa aacgagcaga ttaaagaagg tcacgacttg acagaagaag
    12241 aagaactaac ggtgttgtct cgcgaaatga aacagcgacg tgattctttg catgagtttg
    12301 aagaagcagg acgtgacgat ttagctgaaa aagtaaaaag tgaaattgta atagtcgaaa
    12361 aatatatgcc ggaacaactt tccgacgaag aaattcgaca acttgttcaa gaagcaatca
    12421 ctcaaactgg tgcttcatca atgaaagaat ttggcaaagt tatgggtgca attatgccta
    12481 aagtcaaagg aaaagcagat ggaaaccaag tgaatgcgat tgtgaaagaa ctattacaat
    12541 cttaatttta tcatttaaac tgcggacagt gattgtccgc agt
//
LOCUS       DAFEOM010000075        12300 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.078, whole genome shotgun sequence.
ACCESSION   DAFEOM010000075
VERSION     DAFEOM010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.078"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            31..1062
                     /locus_tag="LP524_002309"
     CDS             31..1062
                     /locus_tag="LP524_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBL2235329.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            1059..1826
                     /locus_tag="LP524_002310"
     CDS             1059..1826
                     /locus_tag="LP524_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBL2235330.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            1926..3494
                     /gene="liaX"
                     /locus_tag="LP524_002311"
     CDS             1926..3494
                     /gene="liaX"
                     /locus_tag="LP524_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HBL2235331.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            3791..4111
                     /locus_tag="LP524_002312"
     CDS             3791..4111
                     /locus_tag="LP524_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HBL2235332.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            4111..4461
                     /locus_tag="LP524_002313"
     CDS             4111..4461
                     /locus_tag="LP524_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBL2235333.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            4628..5566
                     /locus_tag="LP524_002314"
     CDS             4628..5566
                     /locus_tag="LP524_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBL2235334.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            5581..6411
                     /locus_tag="LP524_002315"
     CDS             5581..6411
                     /locus_tag="LP524_002315"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBL2235335.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            6455..7480
                     /locus_tag="LP524_002316"
     CDS             6455..7480
                     /locus_tag="LP524_002316"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBL2235336.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            7495..8433
                     /gene="galU"
                     /locus_tag="LP524_002317"
     CDS             7495..8433
                     /gene="galU"
                     /locus_tag="LP524_002317"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBL2235337.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            8558..8998
                     /locus_tag="LP524_002318"
     CDS             8558..8998
                     /locus_tag="LP524_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBL2235338.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            9001..9603
                     /locus_tag="LP524_002319"
     CDS             9001..9603
                     /locus_tag="LP524_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBL2235339.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(9662..10768)
                     /locus_tag="LP524_002320"
     CDS             complement(9662..10768)
                     /locus_tag="LP524_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBL2235340.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            10978..11979
                     /gene="ccpA"
                     /locus_tag="LP524_002321"
     CDS             10978..11979
                     /gene="ccpA"
                     /locus_tag="LP524_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HBL2235341.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
BASE COUNT         4088 a         2118 c         2594 g         3500 t
ORIGIN      
        1 cttttcatca tttcatagaa aggataaaga atgcaaaaaa aaattagcat catcactata
       61 ttttttattt gtgtgcttac ttcatgggtt attcagcatc aagccgcttt atatgaagta
      121 cctgtcatcg agatagaaaa agtacgaata aaagaggatc atcaattgat tacaggaaaa
      181 ctgatgaaca cggatcaaaa aggagaatcg atttatttat cggttcctta tcaaaaaaat
      241 caaatagaaa cagcaaatat aaaagcgaga caaatgtatc tagtccaaat gcatggagat
      301 gaatggcagc tagtagcaaa aaaaaatgat ggctggcttt tcttcttcac aagtatcttc
      361 attggcgcca tgttgcttat tggtggaaaa cagggtctat ttgctttagg aagtctcatc
      421 atcaatatcg ttttactttt acttttgttg ctcatcttta ctaaaacaga agggatttct
      481 ctattaaata tatctatcct gttcgtcgtt atagggatga ttattagcat attggcttta
      541 gatggatgga atagaagcag tataatcaaa atttcagctg cagttacgag tgttttttta
      601 gcatttttta tttgtctttt gacaatgaat cattggaaag accaaggact gcgctatgag
      661 gaactgaatt atctgacacg accgtatcgt tctgtttttc tttctagcgt actgattgga
      721 acagctggag gaacactcga taccgttatc acagtcattg cgacattaga agaattgact
      781 aagcgacaac cagaaataac agcgaaacag ttatggcttt caggaaggca agtgggtcga
      841 gatgtgattg gctcgatgac caatatcctt ttgttcgtct atatcagtgg gtcagttcca
      901 tcgcttcttt tatatttagg aaatcaatgg tcgttcaaag aaacgataga acagcattta
      961 tcattggaat ttttacgcgt cattgctggc agcttaggaa tagtattatc gatccctatc
     1021 tcggtcctct tttttctgac tgtaaggagg ctgaaacgat gaatgtactt attatacttg
     1081 ttgggatttt agcaatcagc gtgctactta ttggcggtac gcaaggtatg tatattcttt
     1141 taggcttatt catcaattta gggatctttt tcctgttgtt attcggttat catcagaagt
     1201 ggccgatact ggcattgtcg gtcataggat ttttattgat tgctgtggtt atcttatttt
     1261 ttatcaacgg ttataatcta aaaatgcgtg cagcttttgt gagtatactg atctttttat
     1321 tttgtttcct actattgctt cctatcactg attttttagc aatccaaggt tttacatcgg
     1381 tagaactaga agaattatct ggcttagata agactttagc gattgatttt agattactta
     1441 cgagatcgtt gcttctgatt agtcttagtg gtgctgtttt agatgcgagt gtagcaattt
     1501 ctagtgggac attcgaagta tatcaggcaa atccgcaatt atctttttac caattgcgcc
     1561 attctagttt cgctattgct aaaaaaatat tggcgtcaac agtaatgaca cttctttttg
     1621 cattcatggg aagttcttta gcgttgatcc tctggtttat tgatttagac attccttttc
     1681 agcaagtgat caatgaaaaa tcatttgttc tagaatatac gatggctatt cttaccacat
     1741 tgtccgcact acttgttctt cctttaacct gcgtgactac agcatatctc tttacaaaaa
     1801 agaaagaaca actcaagatg gagtaaaagt tacaacttaa gtcctatgac atttttactg
     1861 aatcagtgca taatatggat gtaaggaaaa gcttgcaaac aattttgctt aaggaggcaa
     1921 tttccatgaa agaaagagaa cgtattttag atttagtgaa aaaaggaatc ctttcgactg
     1981 aagaaggctt agatttatta gaaagcatgg caactgaaaa agacgaaaag caaatcaaaa
     2041 aagaagcaga caaggtaact gctagtcata aagaaaaaga tcaggcaagt caattgatcg
     2101 ataaacttga aaacggcgaa gaagaaattt ctgagccagt tgatccaaag gaaagagaaa
     2161 gacaagatca agaaaactta gaaaagatct tagatgaact agcaactgaa gcgaataaaa
     2221 cttcggcgcg tctagatgaa gtaaatgctc aattcgctga caataaggca gcacgcaatg
     2281 agaaacagga agcattgatg cagctgaata caaaagaaga attaggcgag ttgacagaag
     2341 aagaattagc acagcgtcag acgttagaag cagaaatcaa agaattagaa gctagtggcg
     2401 atacactgtt agaagaacaa ttgaaattag aagcagaatt aaaagatatc cgtaagaatc
     2461 aatggtctga gaaaaaagaa acgtttactg ataagtttga attgccagat gattggaaag
     2521 atcaagcaac agatacactg aatcaagtag gcgaaaaaat gtcagaagct ggatcacagt
     2581 taggcaaatt cttgaagaaa acattccaaa ctgtttcaga aacagtgaat gacaacatgg
     2641 aatggaaaga tgtcagctta cgtgtccctg ggattgcgac aacgaaattt gagcatgaat
     2701 tttactatga agctcctgca gcaagtatcc ttgatatcaa ggcagcaaac ggaaacgtga
     2761 cgttaaaaac gtgggacagc aacgatgtga aagttgaagc taagatcaaa ctttacggaa
     2821 aaatgggtgc tgaaccattt gaagcattct ctgaacggag ccagattgag gttaatgaag
     2881 atcatatctc cttccaaatt ccaaataaac gtgtgcgtgc agaccttgtt ttctacttac
     2941 ctaaacgagt atacgaccac gcagctatta aacttctaaa tggcaacatc atgattgaaa
     3001 cattggaagc taaagatatt tatacaaaat ctacgaatgg taacatcatc gtggaccaat
     3061 taactgcaac gatgctggaa gtagaaggtg taaacggcaa tatcgatatt cgaaacggga
     3121 acatcttaga ttctattatc gaaacagtaa atggtacagt gacatttggt gcgacagcag
     3181 aaaacctttc tgtgtctttg gtaaatggtg atgtacgtct tacgattaaa gaagataatt
     3241 tgaaaaaagt agaagcaagt tcagtgaacg gaaatgtcaa agtcgcttta ccagatggca
     3301 tcggtttgga aggacatgct aaaacaagtc ttggaagtat caacagccgt ctgtccaatt
     3361 acgaagtagt tcgtgagaaa aaagaacgga caaaccaaat gttgcaattc cgtcgtgttt
     3421 cagatggtga aatcgcgcaa gtgcaacttt caacgacaac aggaagtatc tatttaaaag
     3481 atacagataa ataaagaggt tgtgaaagag atgttcagcc ttgagcaata agaagcgatt
     3541 ttgtaaaata gcttccccta ttttacaaaa ttgtgcctta ttgccgatag tctatctctt
     3601 gaacaccgtt taccaagaga ttatgaacct gccaattatc gccgagtcac aaggaataat
     3661 ttcagaaaaa gctcgtcacg ttttatgaat atggtaattt gtgaccgaga attttgcaga
     3721 aagacaccga ttaagaagaa ggttgtcaca acgattttct tcatttaaaa tagtagaaca
     3781 gaggtgagac atgatgaaaa aaaagttgac aaaatctaat aaaaatgttg ttttgacagg
     3841 gactttagca ggaataggag aatacatcgg gattgatccg actgttatcc gtgtgcttta
     3901 tgtattcctg agcttagttg cttttggaag tccaattatt ttatacattt tattagcttt
     3961 gattatccca tctgcgcgtt ctgcacgaac aaactatgga cataacaatg catattatga
     4021 aaaaaacaat tatcgtgaac aaactcgtga acaaacaaaa aaacgcaaag aagcggaaaa
     4081 aatcgatgat gacgaatgga gtgactttta atgtcgtact ttcaacgtat tttagttaat
     4141 acgttaacat tcatatcatt ggctgttctt tttcctgcga tggtccatgt gagcagtatt
     4201 tggacggccg ttcttgccag cttcgtattg tctgtgttga atatgtttgt caaaccgatt
     4261 ttgacgatac tgtccctgcc ctttacatta gtcacattgg gattgtttac gtttgttatc
     4321 aatgcagcga ttcttaaaat gacctccttt tttgtgggag cagaacattt tagtttttct
     4381 tcattcggtg cagcgttgtt agtagctatc ttgatgtcgc ttgtcaatgt aattgtcagc
     4441 gaacataaca gtagaaaata aaagaaagct gtgccgaact atcggcgcag tttttttatt
     4501 tagaatcagg aatgcgtttt ctattacatt ggtaattaaa taaaaacgtt ttttttaatt
     4561 ggtagtactt ttggctagac aatgataaaa tgatagagaa cactctaata gaaatgaggt
     4621 agcatgaatg tcagaagttg taaaagttag ccaattagtg aaaaaattat ctttggaaat
     4681 cgtgacaggc gacgagaaaa gcttagaccg tgtcatcgta actggggaca tctcacgccc
     4741 aggactagag ttgactggtt attttaatta ttattcccat aatcgtttac agctatttgg
     4801 cagtaaagaa atcagttttg cggaacggat gatcccagaa gagcgtttga tcatcatgag
     4861 aagattatgt agtgaagata tgcccgcatt tattatttca agaggattac cagctccaga
     4921 agaaatgatc caggcagcaa atgaaaatgg gatagctgta ctgcgttctc ccatctcaac
     4981 ttctcgttta ttaggagaat tatccagtta tttagatggt cgccttgccc ctcgaacaag
     5041 tgtccacggt gttttagtgg atgtttacgg attaggtgta ctgattcaag gtgacagtgg
     5101 gataggaaaa agtgaaacag cgttggagtt gatcaaacgc ggccatcgat tgatcgcaga
     5161 cgatcgagta gatgtttatc agcaagatga attaacggtg atcggagaac cgcctaaaat
     5221 cctggaacat ctgattgaaa tccgaggaat cgggatcatt gatgtaatga atctttttgg
     5281 tgcgagtgcg gtacgtggct ctatgcaagt ccagttggct gtttatctgg aagcatgggc
     5341 aaaagataag aaatacgacc gtctgggctc tgaagacacg accgttgaaa tcgcggaagt
     5401 gggcattcca caaataaaaa tccctgtcaa aacaggacga aacgttgcaa tcatcattga
     5461 agtagccgcg atgaatttcc gtgcacgtac aatgggcttt gacgcaacga aaacatttga
     5521 agaaagatta agtcgcttga ttgaagaaaa ctcaggcaac gactagcaag gagtgacaat
     5581 atgttagcac aaatcaatcg agttgctttt gagcttgtag ggattcctat ctactggtat
     5641 gcattgatca tcgtatcagg tatcattatc gctatgtggc ttagcagcag agaggctgtt
     5701 cgggtaggat taaaagcaga tgatgtgaca gattttatgt tgtggggatt gcctatctcc
     5761 attattggcg ctcgcttata ttatgtttta tttgatcttc cccagtatat agctgatcca
     5821 atccaaatat tcaatatccg ttcaggaggc ttagctattt atggcggact gattgcaggc
     5881 gcgatcacgc tgtatttttt tactaaatat cattttattt ctttgtggac ttttttagat
     5941 atcgctgcac caagtgtatt attagcacag gcaatcggtc gttggggaaa tttcatgaac
     6001 cacgaagcat atggaccaga cacgacgcga agcttcttag aaagtctgca tttaccgcaa
     6061 ttcatcattg ataatatgta tatcgaaggg atctatcgtc aaccaacatt tctttatgaa
     6121 tcactttgga gtttgattgg atttattttg cttttgtttt tacgaaagaa accacacctt
     6181 ttgaaaaaag gcgaagtcct tttatgttat ctaatgtggt atagtttcgg gcgtttcttt
     6241 attgaaggat tacgtacaga cagtctttac tttttaggtg tgatccgtat ttctcaatta
     6301 ttatctgctg tgttatttgt cggtacattg attctattta tttggcgcag aaaaaaagga
     6361 aatttaccgg attatgaccg ctcattagga aaaaatcaac aaattatctg atttttagag
     6421 cgttaaaaat gaaaaaaatt tcaggagtgg taaaatgaat tatagagtag cggttttagg
     6481 accaggctct tggggtactg ctttggcaca agtcctcgcc gaaaacggac atgacgttcg
     6541 gatttgggga catcgtgctt cacaagtaga agaaatcaac acccaacaca cgaatcaacg
     6601 ttatttacca gatcattatc tacccgattc catccaagca ttcaccaata tggaagatgc
     6661 agtaaaagac gcagatgcgg tgctattcgt cattccaacg aaagcaatac gttctgttgc
     6721 aaaagaacta gtgcctaaat tgaatacaaa gccagtgatc atccatgcaa gtaaaggatt
     6781 agaacaggga acacataaaa ggatctctga catcttgtta gaggaaatcc cagcagaaaa
     6841 aagaaaagct gttgttgttc tttctggacc aagccatgca gaagaagtag caaaacatga
     6901 catcactacg atcacagctg caagtacaga tgcaaaagca gcaaaatatg tacaagagct
     6961 gttcatgaat aattattttc ggatctatac caatcctgat gtgatcggtg tagagatggg
     7021 ggcagctttg aaaaatatta ttgctattgg tgctggcgct attcacggct tagggtttgg
     7081 cgataatgca aaggcagcaa tcatgacgcg cggattagct gagatcagcc gtttaggtgt
     7141 agcaatgggt gccaacccgc tgacctttat cggattaagc ggaataggcg atctagtcgt
     7201 tacttgcaca agtgttcatt ctaggaactg gcgcgcaggc aacttgttag gacaagggca
     7261 gcccctagaa gaggtgctag ccaatatggg aatggtcgtt gaaggtgtga atactaccca
     7321 agcagcgatg gaactagccc aaatgatgga aatcgaaatg cccatcactc aaacaatcta
     7381 tgaagtgctg tatgaacaaa aagaaatcaa aacagccgca aaagaaatca tgttacgtga
     7441 tggaaaaaaa gaaaatgaat tctcattaac aattttttga aggagcgaat gtatatgaaa
     7501 gttaaaaaag cagttattcc agcagcagga ttaggaacaa gatttttacc agcaacaaaa
     7561 gctatggcaa aggaaatgtt gccaattgtt gataaaccaa caatccagtt catcgttgaa
     7621 gaagcgttga aatcaggtat cgaagacatc ttgatcgtta caggaaaagc taaacgacca
     7681 atcgaagatc actttgattc aaatattgaa ttagaaacaa acctaagtga aaaaggaaag
     7741 accgaattat tgaaattagt agaagaaacg acagatgtca acttgcattt catccgtcaa
     7801 tctcatccaa agggtttagg acatgccgtt cttcaagcaa gagcctttat tggaaatgaa
     7861 ccatttgtcg ttatgctagg cgatgatata atggaagacg aagtaccatt gacacagcaa
     7921 ttaatcgatg attacgaacg tactcatgct tctacgattg ccgttatgca agttcctcat
     7981 gatgaaactt caaaatacgg aattatcaat ccaggagaag tattggaaga tgggttatac
     8041 aatgtcaaaa actttgttga aaaaccagat ccaagcaaag caccaagtga tttagctatt
     8101 attggacgtt acttattgac tccagaaatt tttgatgtat tgtccgagca acaaccagga
     8161 gccggaaatg aaatccaact aactgacgca atcgatacat tgaataaaac acaacgcgta
     8221 tttgcccgta aattcacagg taaacgttac gatgtcggtg acaaatttgg ttttatgaaa
     8281 acaagtattg aatatggtct aacacatcca gaagtaggtg cacctttacg tgagtacatc
     8341 ctgtctttag gtaaaacatt agaagcagaa ggaagcaaga tcgatcataa tccaaaagga
     8401 ataacagaaa tcaaagaaga aaagaaaaaa taaccctttt ttctaacgag cttcagctgc
     8461 taattgaata aaaagtagtt gaagctcgtt tttatttgtt taaaaaacac gaaaatgcta
     8521 tccttataga aaagagagac aagaagggag tttgatcatg tcaggaggag aaattgcagg
     8581 attgatcgca gcaatcgcct ttgcgatact tgtcgttttt atcgttcgtg tgttgttgca
     8641 agtttctaaa actttagaaa aagtagatca gactgtagca gaagcaaata cgacgatcga
     8701 aatcgtcaca aaagacgtag atcttttgtc tagacaagta gaaggcttac tggtcaaaag
     8761 taatgaactg ctcttggata tcaatggaaa agtcgcaacg atcgatccat tgttcacagc
     8821 agttgctgat ttaagtgaaa gtgtatcaga acttaatcat tctagtaaaa acatcgcgac
     8881 gaaagtcggc ggaatcggta aaacgacagc taaagcgacg gttgccggaa aagtcggtgg
     8941 gacagcgatg aaatttttta aaaacaaaaa acatactgaa acgacaggag gaaaataaaa
     9001 atggctaaaa aaggtggatt tttattaggt gcagtagtcg gaggcacagc cgcagcaatc
     9061 gccgcgttat tgttagcacc aaaatctggt aaagaacttc gggatgaatt agctgcccaa
     9121 gcggatgatt tgatggatat ggcttcagat tacacagata tggcgaaaga aaaatcgact
     9181 gagttaacag gtattgcaaa agaaaaagcg caagatcttt ctcaacaagc aggtgatcta
     9241 gcaggtagtg tcaaacaaaa agtaagtgaa tcagcagatg aagcaggaag tgaatctgac
     9301 gatatcttgt taaatcttaa agaacaatca gctgacttat ctaatcgttt caaaaaaacg
     9361 gcagaagata tgaaaaaacc tgcagatgaa ttaggaagta ttgctaaaga tacttcagaa
     9421 gatatcatga ttgatgtaaa agacgctgct ggtaaagcag gagatactgt caatgaagga
     9481 atgtcagaag caaaaccagt tgcagatgac tcaaaagaaa tgatgaaaat ggcaaaagaa
     9541 gatgcgaaag aagctaaaaa agaaataaaa gatacaatga cagattcatc tacaaatcac
     9601 taaaatcaca aatttataga ataaaaaggt gggagcggac tagctctcac cttttttact
     9661 cttatttttc taaaacaagt aattcttttg gtgtagtcgt gaatggcaca cagccatcgc
     9721 ttgtcacata tacacagtct tcgatacgta cccctacttt ttcaggaata tagattcctg
     9781 gttcaagaga gaagcacatc ccttcttcga ttactaagtc atttccttct acaagggaag
     9841 ggaattcgtg gacggttgtt ccgatcccgt gacctaaacg gtgattgaaa tattcgccgt
     9901 agccatagct gttgatcacg tttcgtgcga tttgatcaag ttcacctgct gtcacacctg
     9961 gacggacggc ttcttgtgcg gcaagctgtg cttcaagagt gatgttgtag attttttctt
    10021 ggaagtcggt tggtttttga taagcaattg tacgagtagc atcactgcaa tagccattcc
    10081 aaacaacgcc aagatcgaat aacacaagtt cattcggtga aatcttcgta tttcctggtg
    10141 taccatgagg gcttgcagca tttgctcctg caaggactaa tgtgtcgaaa gacatttgtg
    10201 aaacaccttg ttttttcaat tggtattcaa tttctgcaat gatcgcttgt tcagtcacgc
    10261 cttctttcac tgctttaaag ccaatctcaa atgcaacatc ggcccattta ccagcttcga
    10321 tcagtttgtc tttttcctct agagttttga tcagttgtag acgttggatg atggaagtta
    10381 gatcagtgta agaatccgtt cccgtcaaga tatcagagat agcatcgaag cgctcgacag
    10441 tcaaggcgtt cttttcaatc cctacattgc caggctgacc atagcgagca cgaagttctt
    10501 ttccgatgat ctcccaaggg ttttcgctgt ctaaatagcc acggacatca tacgcccaac
    10561 tactgttttg agcatcttca acttccaaag caggagtgaa aaggaaagga tcttgttcaa
    10621 gagggataaa aagagctagt acacgctcat gaggatcact ttcgtatccg gaaaagtaag
    10681 cgatgtggcc agggtcgctg atataagcca attcgacttg gtgttgtgcc atccattgac
    10741 gtagttcatt gatttttacg ttattcattt ggcaacctac tttctaaatt aattttgtat
    10801 tcaataattg taccactgat ttattaaaag accaacgttc taaaaactga aaaaagtaaa
    10861 aattttcatg aaaacagaga taaaacggtt gcaattcttg agattatttg atattctatt
    10921 ttagaaatgt aagacgtatt ttcagaaaac atataaaata tgaataggag aacacaaatg
    10981 gaaaagcaaa caatcactat ctatgatgta gcgagagagg caaatgtttc gatggctact
    11041 gtttcacgtg tcgtaaatgg caatccaaac gttaaaccag caacaagaaa aaaagtcttg
    11101 gaagttatcg atcgcttgga ttatcgtcca aacgcagttg cgcgtggctt ggcaagtaag
    11161 aaaacaacaa cagttggtgt aatcatccct gatgtcagca atatgttctt tgcttcttta
    11221 gcacgtggta tcgatgatgt ggcaacaatg tacaaataca atattatctt agcaaattca
    11281 gatggcaacg accaaaaaga agtcaatgta ttgaataact tactggctaa gcaagtggac
    11341 ggcgtgatct tcatgggtca ccatatcaca gacgaaatcc gtggagaatt ttcacgttca
    11401 aaaactcctg ttgtattagc tggctctatt gatccagatg aacaagtagg tagcgtaaac
    11461 attgattatg tatccgcaac aaaagatgca gtaaacaaat tggcaaaaag cggcaataaa
    11521 aaaatcgctt ttgtcagtgg tgcgttgatc gatcccatca atggacaaaa tcgtctgaaa
    11581 ggctataaag aagcattaag cgaaaataac ctaccatata gcgaaggatt gatctttgaa
    11641 gcacaataca acttcaaaga tggtctatct ctagtcgatc ggatccataa tagcggtgca
    11701 acagctgtct atgtttcaga tgatgaatta gccatcggta ttttggatgg tttattagac
    11761 cgtggcgtga aagtaccgga agaattcgaa atcatcacaa gtaacaattc actactgaca
    11821 gaagtagctc gtcctcgttt aacaagtatc acacaaccat tatacgattt aggggcggta
    11881 tctatgcgcc tattgacgaa aatgatgaat aaagaagaag tcgaagaaaa aacgattatc
    11941 ttgccttatg gaatcgaaga aaaaggatct acaaaataat aaaaggacaa ccattcagag
    12001 gttgtgtgcc tgccgtttaa ctctgcgtta caaggagcaa tttcaaaaaa cagcatctca
    12061 tgtttgatga atattgtgac tcgtgattcg gagttggcag gagaacaccg tttatttggg
    12121 aaaaggggtt atgacaaaat tttgttataa tccttttttt gtgtcttaaa aaagagagaa
    12181 agtggttgaa attacattta tcgtatataa aacaatccgt gtatagatgc tgcattttgt
    12241 tcaccaccat caaaaaaata agggactgtg acaatactat tgtcacaatc ccttattact
//
LOCUS       DAFEOM010000076        12245 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.079, whole genome shotgun sequence.
ACCESSION   DAFEOM010000076
VERSION     DAFEOM010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.079"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(93..968)
                     /locus_tag="LP524_002322"
     CDS             complement(93..968)
                     /locus_tag="LP524_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBL2235342.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(1050..1841)
                     /gene="codY"
                     /locus_tag="LP524_002323"
     CDS             complement(1050..1841)
                     /gene="codY"
                     /locus_tag="LP524_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBL2235343.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(1859..3259)
                     /gene="hslU"
                     /locus_tag="LP524_002324"
     CDS             complement(1859..3259)
                     /gene="hslU"
                     /locus_tag="LP524_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HBL2235344.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(3273..3821)
                     /gene="hslV"
                     /locus_tag="LP524_002325"
     CDS             complement(3273..3821)
                     /gene="hslV"
                     /locus_tag="LP524_002325"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HBL2235345.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(3858..4763)
                     /gene="xerC"
                     /locus_tag="LP524_002326"
     CDS             complement(3858..4763)
                     /gene="xerC"
                     /locus_tag="LP524_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBL2235346.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(4961..6337)
                     /gene="trmFO"
                     /locus_tag="LP524_002327"
     CDS             complement(4961..6337)
                     /gene="trmFO"
                     /locus_tag="LP524_002327"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HBL2235347.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(6306..8384)
                     /gene="topA"
                     /locus_tag="LP524_002328"
     CDS             complement(6306..8384)
                     /gene="topA"
                     /locus_tag="LP524_002328"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBL2235348.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(8506..9363)
                     /gene="dprA"
                     /locus_tag="LP524_002329"
     CDS             complement(8506..9363)
                     /gene="dprA"
                     /locus_tag="LP524_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBL2235349.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(9419..10186)
                     /locus_tag="LP524_002330"
     CDS             complement(9419..10186)
                     /locus_tag="LP524_002330"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBL2235350.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(10179..11039)
                     /gene="ylqF"
                     /locus_tag="LP524_002331"
     CDS             complement(10179..11039)
                     /gene="ylqF"
                     /locus_tag="LP524_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBL2235351.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            11299..11697
                     /locus_tag="LP524_002332"
     CDS             11299..11697
                     /locus_tag="LP524_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL2235352.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            11934..>12245
                     /locus_tag="LP524_002333"
     CDS             11934..>12245
                     /locus_tag="LP524_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL2235353.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LY"
BASE COUNT         3225 a         2639 c         2150 g         4231 t
ORIGIN      
        1 aatcgatgtc acactccctt attacttaca ggatagcttt tttacacacc ttggtaaacg
       61 gtgttccctg ctaacttctc ggtcacaacc tcttacttca ctgtgatgga atattttgtc
      121 ttgaagattt catgggcacc tagttgattg atccctactt tttccatcaa gtttccacta
      181 ctgttcaccg tgtcagcgat tccgcaccac ggttcgatac aaacgaaagg cgcttctttt
      241 ggataaggag accagatccc tacataaggc atatctttat agcttaaagt tacgctatgg
      301 gaagattttt cacttcgaat ggcgtacgca tttagacccc ttgtttcaaa aaccaaggca
      361 tcttgctcaa aaagttcacg attcaaagcc agattcgtat ttgtctgacc taacgtcttt
      421 tgatccatat cgatgaatgg gcctttcaat ggtaggcgta atcttgattt caatggagaa
      481 aatgccagat agaaatcttc aaatgacaat ccttcttcaa gaggtacatt gaaagcagga
      541 tgtccaccga tagaaaagaa catttctttt tcaccggtat ttgccacttg ataatgaacc
      601 gtgatgccgt ctcctcctaa ttcatagctg atcactaatt caaaatcaaa tggatacttt
      661 tctaatgtct ccggcgtact ccttaagatg aaactagcca tttcttgtcc atgttcaatg
      721 acttcaaaat ccatatcccg agcaaatccg tgttgcccca tttgatatgt tttcccttga
      781 taaacatact gatcatcttt taaacgtcca acaaaaggga ataaaaccgg tgcatgtctg
      841 ccccaatacg ctggatcacc ttgccagata tactctaaac catgttcttt tgatttcaga
      901 ctgatcaatt ccgcaccgtg ttcagcgatt tcggcgatca gctggtcatt ttgaataata
      961 acagtcattg ccattcctcc aacttcgact tactttttaa aatgggaagg ctggaacaaa
     1021 catttctatc tgccccagtc cccttacttc taataactgt gttttgctaa ctcgtcttta
     1081 aaacgggaat tcaatacttt taaataagtt cccttcatgc ctaacgaacg agattcaatg
     1141 attccagctg attccaattt tcgtaaagca ttgacgatca cagaacgagt gatgccgata
     1201 ctatcggcaa tattagaagc agtcagacga ccttcatctc catccaatgc ttcaaagata
     1261 gcttgtacag ccttcaattc gctataagat aatgtgttga ttgccatttg gacagccgtt
     1321 gcactccgta catctgcttc gatgcttcga gacttctgat aaaggatctg cataccgaca
     1381 accgtagctc catattctgc taagactaga tcgtcatctg caaacgattg ttccatacga
     1441 gataagataa tcgtacccaa acgttcgcca gctccaaaga tcggaacgac agtcgtcaga
     1501 ccaaatggat atttctctct tgactctacc ggaaatgcag taatatcact agtaattggg
     1561 atattagctt cagttttcat caactgtgcc actgtatcag tataagattc aggaaattgt
     1621 ttctcaacaa acattgtttt gattcgttca ttgttcacat cgtgacgttc attataacct
     1681 agtaatactc cgtcacttcc tatgatatag gtgttgctgt caagaatacc acctagtgtg
     1741 acagccattt tatcgtaagg caaatctgct tgcatgtcaa aagtattctt ttgctgcaaa
     1801 agtgtgttta tcttgcgggt tttcgttagc aagtctgtca ttgtcgtcct ccgatttttt
     1861 ataaaatata gcgacttaaa tcttcatttg ccgcaattga gccaagtttg tcatccacat
     1921 atgcttctgt gatcgttatt tctcccattt gcatatctgg tgcttcaaat aatagatctt
     1981 ctagtagttt ttccaaaatg gtatgcagtc tgcgtgcgcc gatattatct gtgtcacggt
     2041 tgacatcgaa agcgatttga gcgatccgtt caattgcttc ttttgtaaac gtaactgaca
     2101 cattttctgt accgatcaac gcgacgtatt gttttactaa agcattattt ggttcggtca
     2161 gaatcttcac aaaatcttca gctgtcaaat catctaattc tacccgtatc gggaagcgtc
     2221 cttgaagttc tggaattaga tcacttggtt tagacaaatg gaaagctcct gaagcaatga
     2281 ataggacgtg atctgtttgg atgatcccgt attttgtgtt tacttgtgaa ccttcgacaa
     2341 ttggcaatat gtctctttgg acgccttctc tggatacttc tccagattgc tgtgatttag
     2401 aagtgatctt atcgatttca tcgataaaaa taattccact ttcttccgct aatttgattg
     2461 cctcactatg gatatcagca tccttgacta attttgctga ttcttccttg atcaataact
     2521 ctaaagcttc tttgactgtt accgtccgtt ccactttttt cttcggtgtc aaagcaccta
     2581 gagtttcatt taggtcgatc cccatttgct ccaatccatt gttcatcatt ggtgcaggtt
     2641 tcttttggtc ttcaacttca atggttactt cacgactatc cagtaaaccc ttttgtagtt
     2701 gttccaagac tgtacggcga ttgattcgaa tatcttctgt cacttcttct tgcggttcag
     2761 cggtattttg ctgactagca ttaaaaagat tcatcatttg ttcaaattga ttagtggatt
     2821 gtttctgttc ttttttgata ccaggtacca agatttttac caaacgtttt tctgcatttt
     2881 ttttagcact tgtgtataca cggctgtact gctctttttc tacgatttgg atcgcatttt
     2941 ctactaaatc ccgaaccatt gattctacat ctcttccgac ataaccgact tcagtaaatt
     3001 ttgttgcttc gactttgaca aatggtgcat tgacgatttt tgccaaacgt cgtgcgattt
     3061 ctgttttacc gacccctgta ggtccgatca tcagcatatt ttttggtgta atatcttgtt
     3121 gcattttttc atctaactgc aaacggcgat agcggttacg caaagcaacg gcaacagatt
     3181 tttttgctgc gtgctgtcct acgatatatt catctaattc acttacgatt tgtcttggtg
     3241 tttgttttaa ttcatacata tctactcacc tcttacagtt cttctacgat aatattatga
     3301 ttagtaaaga tacagatatc tgcggcaata tttaacgcat tttctgcaat ttctttcgca
     3361 ctcatgtcgt tttgagcata gtttttcatt gctcgggctg cagccaacgc aaaattccct
     3421 cctgatccga tagctaagat accatcatct ggtgtgataa cttctcctgt acctgaaaca
     3481 agaagcattt ctttatcatt cattacgatc agcatcgctt caagtttttg catagattgc
     3541 tgtgtccgcc attcttgagc taattcgaca gctgcgcgtt gtagattccc gttatattcg
     3601 tttagtttgc cttcaaattt ttcttctaaa gtaaacgcat ctgcaacact tccggcgaaa
     3661 cctacaacaa cttctccatt gtagatacgt cgtactttct ttgcagtgcc tttcatgaca
     3721 acagattcac ccattgttac ttggccatct ccagccatag caaatttccc gtctttttcg
     3781 acggcacaga tagtggttga atgaaatggt gattcgacca tgtttgtttc ctcctaaatt
     3841 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
     3901 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
     3961 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
     4021 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
     4081 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
     4141 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
     4201 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
     4261 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
     4321 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
     4381 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
     4441 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
     4501 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
     4561 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
     4621 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
     4681 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
     4741 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
     4801 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     4861 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     4921 agagcgagac tatccgtctc accctcttca tggagtttcc ttactcattt gggattgcag
     4981 ttttttctaa ttctgttttt attgcctcat tagcttttaa agctcgttgt gcaattgctt
     5041 catatcttgc ttttttgtct cgaatgcgct ctggcagttc aggaaacaga ccatagttgg
     5101 cattcatcgg ttggaaatgt tttccttccg catgagtaat gtagtaagcc atacttccaa
     5161 tagctgtttc ttgtggaaac tcgactggtt ccatttcttt tgctaagcgt gcggcgttga
     5221 ttccagcaag tagtccgctc gctgcacttt caacatagcc ttctacacca gtcatctgac
     5281 ctgcaaagaa cagatcttcg cgtttcgttg attggtaagt cggtttcagc aattctggcg
     5341 aattcataaa gctgtttcgg tgcatcacac catagcgaac gaattcagca ttttctaacc
     5401 caggaatcat ccggaaaaca cgtttttgct cgccccattt caagtgagtc tgaaagccca
     5461 cgatgttgta taaagaagct gcagcattgt cttgacgcag ctggataaca gcataaggac
     5521 gtttgccagt tttaggatct tccaatccaa ctggtttcat aggtccgaaa agcatcgttt
     5581 tgaatcctcg ctgtgccatt acttcaattg gcatacatcc ttcaaaatat ttttctttct
     5641 caaaagattt aagttctgcc acttcggcat tgattagtgc ctcatggaaa gctttgaatt
     5701 cctcttctgt catcggacag ttcaagtaag ctgcttctcc tttgtcatag cgggacttca
     5761 aatatacttt atccatatcg atcgtagctt tgtctacgat tggtgcagct gcatcataga
     5821 aataaaaacc atctgaacca ttgaatgctt tgatgctttc tgctaatgga tgagaagtca
     5881 aaggtcctgt tgctactacg gtgattcctt ccggtatttc agtaatttct tcgtttatca
     5941 ctgttactaa tggatgctct ttgacttttc gggtaattgt ttcagaaaaa gaatcgcggt
     6001 caacagctaa ggcaccacca gctggcactg ctgtttcatc tgccgaagtg attactaccg
     6061 aattcaatcg gcgcatttct tctttcaata accctactgc attagccaaa ctgtttcctc
     6121 ttaatgaatt ggaacatact agttcagcaa aattacttgt gtgatgcgcc tcagtcgatt
     6181 tgactggacg catttcatat aaacggacag ggactcctgc ttgtgcgatc tgccaagcag
     6241 cttcacttcc ggcaagccct gctccaacaa catttaccat tttcaatgaa agaacctctt
     6301 ttctattatt tttggatgtc ttcttcgtaa tctccattta cacagactac ttgtttaccg
     6361 cctttgactt tcttctcgac cagatatccg tcacattttg gacaatttct gcccactggt
     6421 ttatcccaag aagtgaattc acattctggg aaacgggaac aaccatagaa tagtctgttt
     6481 ttcttagatt ttcgttcaat gacttgtcct tcatgacata ctgggcaagt cacaccgatt
     6541 tcttttacga ttggtttcgt attccgacaa tcaggaaaat tactgcaagc ataaaacttc
     6601 ccatatttcc ctaattttat caccatcgga tgtccacaga catcacaatc gaatcctgct
     6661 ggttcatctt tgatttgaat tttttcgatt ttttcttccg cattcgtcag ttctttttca
     6721 aacggtttat agaaacgatc aaccactttg acccacgctt ccgtgccttc ttcaatttta
     6781 tccaaatctc cttccatttc tgctgtgaaa tggatatcga cgatttgagg gaagaattca
     6841 cagattaatg aattgacaat ttctcctagt tcagtcggct caaatctttt ttgtgtcaat
     6901 tttacatagt agcgtctttg gatcgtatca agtgttggtg cataagtgga aggtcgccct
     6961 accccgtttt cttctagtgc acgaatcaac gtagcctcac taaaacgtgc tggtggttga
     7021 gtaaaatgtt gttttggttc aatatcaaca gattggacta catcgccttc ttcaagttct
     7081 ggaagaatat tttctttctc ttctttgccg tcatctctac cttccacata cacttgcatg
     7141 aatcctttga atttgatttt tgaaccgttc gcaatgaaga tgacaccatt ttgttctaat
     7201 gtcactttca ttgtatctaa aatagctggt gtcatctgac tagccacaaa acgtgaccag
     7261 atcaatgtat aaagtttcaa ttgatcttta tctaaatatt gtttgagttc atctggtgta
     7321 cgaagcacac tcgttggacg cacagcttca tgggcatctt gtgcgccttg agcatttttg
     7381 atctttcggt gatcatgtct agaaaattct tttccatacg tatcttcaat aaatgctgcg
     7441 gcctctgctt ttgctgtatc ggcaatccgg gtagaatcag tacgcatgta tgtaatcaag
     7501 ccgactgttc cttgtttacc caaagtgatt ccttcatata attgctgtgc taccatcatg
     7561 gtttttcttg ttcgaaagtt taacttacgg gcagcttctt gttgcaggct acttgtagtg
     7621 aaaggattcg cagggtttct tttgcgctct ttcttttcta cttttttaac ttcgaagtct
     7681 gttccatcaa ttctagcagt gacttctttt acactttgtg catctggtaa tttctttttc
     7741 ttaccgtcga ttccccagaa atttgctttg aatttcttgc gagctttttt aaaattaccg
     7801 tcgatgctcc aatattcttc tggaacaaat tctcggatct ctttttcacg gtcaataata
     7861 atttttaaag cgacagattg gacacgtcct gcacttaacc ctttcttgac cttacgccag
     7921 agaataggac tgatcgaata accaacgatt ctgtctaagg tacgacgagc ttgctgtgca
     7981 tctactaaat ctacatcgat cgttcttggt tccttaaaag ctgctttgac tgcgtctttt
     8041 gtgatttcat tgaagaccac ccgatttttt tctttcagat ccaacccaag taggtacgct
     8101 aaatgccaag caatcgcttc tccttctcta tccgggtcac ttgccaagta aactttttga
     8161 gctttttttg ctgcggcttt caggcttttg atgacatcgc cttttccgcg tatagaaata
     8221 taatggggtt cataattgtt ttcaaaatcg atacccattt tacttttcgg taaatctctt
     8281 atatggccaa cactggccat tactttatag tttcgtccaa gatatttctc aattgtcttg
     8341 gcttttgcag gtgattctac aatcactaaa tatttatacg ccattactga atggactcct
     8401 ttccattgtc gtgttcctac tattaaaaga ggattcatac aaatcgtagc taatcatatc
     8461 cattcagaaa acgtttgtca agaaaagcaa atgaaaagta tggtgttatt ctttaaattc
     8521 tgccaagata tcttcacccg aaaccgtgca aactgcaccg tcttggatca aatgatgaca
     8581 tccggtagaa tgttctgtta aaatatttcc tggaacagaa aaaactgttc ttccaagatc
     8641 caatgcttgc tgtgctgtta tcagagagcc gctttttttc ttggcttcaa agacacatgt
     8701 tcctttagac aaagctgcga taatccgatt tctcattgga aaacaatgct ttttcgggcc
     8761 ggattgagga ggatattcac tcaaaagaag atgatcattt gcaatcacct cttgcaagtc
     8821 acgattttca gcaggataga cttgctctat ccctgttcca atgactgcga ttgtccgtcc
     8881 accatgagag attgctgctt ggtgaccgta tgtatctatc cctttcgcgc agccactgac
     8941 gatcacatat ccagcttcta tcactggttt tagtaaataa taaactgttt ttttggctaa
     9001 agcagaagtg tgtcttccac caacaacgct taacatgtta caagaaagta aagatagatc
     9061 accattataa aacagtacaa ttggcggata ggtcaagtga agcagctgag ggggatattt
     9121 gggatcaaag caagtgataa acgactgttt cgattggatt ttgccccagt tagcgtgaat
     9181 ttcctgccaa cttttgcgaa acagctcccg gtgtttggtt attttaccta tcttaatgat
     9241 gtcttcaaat ccgatttctt tataaggata ctgcatcatc cagttagcaa gtgcccattt
     9301 tccttgacta ctgattcctt ttgtataagc aagtttcaat aaattttctt ctatttgata
     9361 catcataaaa ccgcctttcg tctctctttc taataagata cgtaaaagcg gctgtttttt
     9421 attgtaaata tttttttaca ggtgcaaaac ttttacgatg gattggcgta attccttgtc
     9481 gttcaagtcc ttctaagtgt tcctttgttc cataacctgc attatgcgag aaaccatatc
     9541 caggataaag cacgtcatat tctttcatca attggtctcg atatacttta gcaatgatac
     9601 ttgcagcagc aatcgacaca gatcgtgcat cccctttgat tattttttcc tgctgaatag
     9661 gcaaatccaa ggtcatcgca tcgatcaaca aatagcttgg tacaatggac aatttttcta
     9721 ctgcttggat catcgctaat ttacttgctt cataaatatt cacatcatcg attgtctgct
     9781 gatcaacgat cccgatcccg attgccaggg ctttttcttg aacttgttga taaagaagtt
     9841 ctcgcttttt ctctgataat tgtttggagt cattcagccc taatatgatc tcatttttcg
     9901 gcaagataac tgcagctgcc acgacaggtc cagctaatgg tcctcttcct acttcatcaa
     9961 tgccggcaat ccattggtga ccttgtgcgt acccttgctt ttcaaagcaa aacatttctt
    10021 catatttttc cttaattaat gcttcctttt tcaatcgtcg ttcaaaagaa gccagtgctt
    10081 gctgcacgcc tttgcgtgga tcatttctta atgattctag tgtaggatca tctattctct
    10141 gtatgctttt caatcgttct ttgatacttt ggatggattc agtcatcact tttttgctcc
    10201 atttcttcgc aacgatccag tgtgtatcgt ccaagttttc cttgacgaat atcatgcacg
    10261 actaattcgc tgccacgatc atagtcttct ttatacccac gttttgctgt aatatccatc
    10321 agtaattccg gtgccaattt ttcttcttgc tcgattgata aaccataacg atcgctgatt
    10381 cttcctgaat aatatcgggc aaaaaagtct aatccgtaaa tcgctaaatc atctaaatgg
    10441 acaagctggt ctttgatcgc gcctgtcagc gctagttttt tgccgatttc ttcatcttca
    10501 aatttgggcc ataaaatccc tggtgtgtct aacaattcca actcattacc taaacgaagc
    10561 cactgctgac cttttgttac gccaggctta tttcctgtct gtgcaatttt cttgcctact
    10621 agtcgattca tcaacgtaga tttcccaaca tttggaatac ctaagaccat tgcccgaata
    10681 gcgcgaggct tgatcccccg agcagcatct cttgcccgtt tttctttcaa tgcttcttta
    10741 gccgcaggta ctagaccttt aattccttta ctttcttgtg ccgtgatagc taatgcaaga
    10801 tggccttgtt tacgaaaata ttcttgccac agctttgtct gttgtggatc tgccagatcc
    10861 gctttattca ataaaacaag gcgaggtttt tgctgaatga tctgatctag cattggatta
    10921 cgagaagata aaggtaatcg tgcgtctacc aattcaaata cgatgtccac atatttcatt
    10981 ttttcagaaa cttctcgtcg ggcttttgcc atatgtcccg gaaaccattg gatggtcatt
    11041 gttctctcca ttcctttcat accggaatat tccccggctt tacttgtttt tttctttttc
    11101 aatataaatt cactttttta ttataaagaa attccatcta ttagttaagt gaaagtgaac
    11161 cagtcctcat tctatcacat cttcactttt ttgatgatgc aaatttttat tttggtttct
    11221 atatatgaat ctaaattttt gacagcgttt cctaaataag agatgataga cgaacaaaac
    11281 agaaaggaga cgaaacaaat gaatagtatg gtttttgtta attttcctgt tgctgatgtc
    11341 aatcgttccg ttgcttttta tgaaaagctg ggttttaaaa aaaatgaaga attttctact
    11401 gaagaagctg gttcaatggt atgggatgac actttttgga taatgcttct ctctcatgat
    11461 ttttataaaa aatttataaa agataaagaa atagctgata ataaaaaaac aagtggtgct
    11521 ttgatttcgt ttagtgttga aaatgcggat gaagtaaaaa aatttgctca aattgcaaaa
    11581 gaaaatggtg gtactttcca cgaagtagat atgggaatgc ctgaagaaga aatgttttca
    11641 ttagaagtcc aagatctaga cggaaataca ttagaacccg tatggatgaa gatgtaagca
    11701 gataactaat ctgacgtttt ttataacaaa aaaacaaagg cggatgatac gataagtatt
    11761 ctccgcctct atttttttta ttcgtacaat ctctttttta cagtttataa aagcagtctt
    11821 ctcacccttt ttttaattgt tagatgccag tgtatgtaaa tcaccaatgt gtcatgacac
    11881 attggtgatt ttatggtatc cttttattat taactgacaa aggagagaat attttgaaca
    11941 ctttgattgc aaaagacgtt tttagattgc ccgaaatgaa tgcaagattg ttgtcgacct
    12001 ctgctctttt attagccctt gatgtcatct tatacaagat agcgattggt cctgcttatt
    12061 ttcaaatcac attcggattt ttaagtatgg cgcttatggg ttatctatat ggaccgatct
    12121 gggctggttt gcttgaaatg ttgtcgaaca ctttaaattt cacgatattt ggttcaggaa
    12181 cgtttcaagc tgcttttctt atcacggctt tccttggtgg atttattaat ggcttatttt
    12241 tatat
//
LOCUS       DAFEOM010000077        11623 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.080, whole genome shotgun sequence.
ACCESSION   DAFEOM010000077
VERSION     DAFEOM010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.080"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(286..2070)
                     /locus_tag="LP524_002334"
     CDS             complement(286..2070)
                     /locus_tag="LP524_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2235354.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            complement(2070..3821)
                     /locus_tag="LP524_002335"
     CDS             complement(2070..3821)
                     /locus_tag="LP524_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL2235355.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            complement(3963..4187)
                     /locus_tag="LP524_002336"
     CDS             complement(3963..4187)
                     /locus_tag="LP524_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HBL2235356.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            4438..6024
                     /locus_tag="LP524_002337"
     CDS             4438..6024
                     /locus_tag="LP524_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBL2235357.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            complement(6090..7865)
                     /locus_tag="LP524_002338"
     CDS             complement(6090..7865)
                     /locus_tag="LP524_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBL2235358.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(8078..8980)
                     /locus_tag="LP524_002339"
     CDS             complement(8078..8980)
                     /locus_tag="LP524_002339"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBL2235359.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            9259..11604
                     /locus_tag="LP524_002340"
     CDS             9259..11604
                     /locus_tag="LP524_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBL2235360.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
BASE COUNT         3545 a         2361 c         2141 g         3576 t
ORIGIN      
        1 caaaaagaaa aacccaaaaa gccgtccatg aaaccatggg agactcatca ggtttagctt
       61 gcatcaggca atcactatcc aagttgtatc gttttcttta tattatcgaa agcaaatgct
      121 tttgtcaacg ctttcttagt ggctattagg cacttctgac tagctgtcga atcttttttt
      181 aaatctggtg actacgacac taactctcac agatgaaaaa tatgagaagc tatgttttat
      241 tttcactcta gtgcttaggt caaaattcta ctgtcacaac atcttttata taccactgct
      301 ttgcagtttg tacatatctg catacaaacc attttgcgct agaagtgttt catgattccc
      361 gcgttctact atatgacctt tgtctaaaac taaaatcaat tctgcatctc gaatcgttga
      421 cagccgatgg gcaatcgcaa tcgttgtccg tccttgacgc atttttgcca atccttcttg
      481 gatcaatcct tctgtttcag tatcgatatt tgctgttgct tcatccaata ctagaatttt
      541 aggatcagtc acgattgttc gcgcaaaagt gatcagctgt cgttgtccac tagaatagct
      601 ggcaccatgt tcgatcactt ttgagtgata tccttttggt aattcatcga tgaatttgtt
      661 tgcttgaaca aactcagccg catggcgtat ctgttcgtct gtgatagctg gattcatcat
      721 ccgaatattt cctgcgatat ccccataaaa caagaaagca tcttgcaaga ccagccccat
      781 tttcttacgt aactcttcca ttggatattc tcgaatgtca tgatcatcaa tcaatatctg
      841 accttcataa aattcgtaaa accgcatcaa tacgttgatg atcgagcttt ttccgcttcc
      901 ggtgtgtccc actaatgcaa cggtctctcc cggattagca acaaaagaga tattgtttaa
      961 tacattgtgc tctccatcat aagaaaagct tacattgcga aactcaatct tacctgaagt
     1021 aatcatttga tctgctccag ggttttgttg tggtgcatat tcttccgtat ccataatctt
     1081 caagatacga ctaccagcaa ccatcccatc tgtgaagatg ctcaaaaagt ccatcatttg
     1141 agacattgga ttaaaaaatc cctggacata ggtcgtaaat gcatagatca gtccaacttc
     1201 tacgggagaa tgcaacgcat cgatcccaaa caaagtcaac accaacgcaa tcgccaatgc
     1261 atatagaaaa ttgatgatcg gacttaacag aatcgaattc gttttgatca tcgcataacg
     1321 cgtgtctaaa tactcattgt tcacttgttc aaattcttta gctaaacgag cttcttggcg
     1381 aaactgctgg ataatacgca tgccggaaat cgattcattc aatttcgtgt tcagctgact
     1441 taatttttcc cgcatatttc ggtaaatacg agaactgaat ttctggtaat accagatgac
     1501 gattcctaaa atcggcaaaa acaccaaagt ccacaaggca atttctccat tgatttgaaa
     1561 catggcaagg aatgaagaaa cgactgcaaa gatcccagtc aataccatca aaaagacgta
     1621 ccaaaattca aatagcgttt ctgtgtcatt cgtcactcga gaaacggttg atcctgccgg
     1681 tgtctgatca aaataacgca tacctagtgt atgcagcttt tcaaataatt ttaccctaat
     1741 atactgatag gtcttcaatg aggccatgga atacaaatac cattggaaaa accaaatgat
     1801 actttttact atgactccaa ataagtacat accagcaaaa aacaaaataa cttgtgtcgt
     1861 agccgactgt ttagccagat gatcatccat aaaagtttgg atgatccttg gcaataggac
     1921 attgatcaca gacaaagcaa acgcaaataa gatagcaacg atgaacgttc ttctaaaagg
     1981 tttggcgaac ttcatcagtc gtttgatgat ctttttctgt tctttaagtg gaatcgattt
     2041 tgtccattcc gattgataat tttcatccat caggcctcgc ctccttctat cttcgcttct
     2101 aattgctgtt tctcccacat ttgttcatac cagccgccta gtgcaagcag ctcatgatgg
     2161 gttcctcgtt caatgatttt cccttgatcg acaacaatga tttcttttgc atgcatcacg
     2221 ctacttaaac ggtgagctgc aatgatagtt gttttctctt ttcttatttc ttttaaatta
     2281 tgcaagatag cttcttctgt tttggcatct actgcagaga gtgcatcatc taatattaat
     2341 aattcaggat ttttgatcaa tgctcgagca atcgaaatcc tttgtttctg tccgccagat
     2401 aaagagactc cacgttctcc aactaaggta tcataacctt ttggtaattc cttgatttca
     2461 ttgttgataa atgctaaaga agccgcatgc tctacttctt cttctgaaag ttttggatta
     2521 gaaaaacgga tattatctcg aatggtcatc gaaaacaaga agtgatcctg aggcacatag
     2581 ccgattgatc ctaataaagc attcaatgaa tattctttga tcgaacgtcc gccaaattgg
     2641 attttccctt gataatcatc gtattcacgc atcagtaatt tgatgatcgt cgtttttccc
     2701 gcaccagttt tcccaacaat acctaatgtt tctccttctt ggatcgcaaa cttgatttgt
     2761 tctaatgctg gtgattcttc tttcggataa ttaaaagagg aaatagctac attcagctcg
     2821 ccttttgccg gggtctgcac tgcacccagt tgttccacga tatgcgtctt ttcatgcaaa
     2881 agttcattga cgcgatcata agaagcattt cctctttcca atacgttgaa caatcgacca
     2941 atcgcaaaca tcggccaaac aagcataccg atataactaa taaatgagac catctgcccg
     3001 atcgagatcg tgccttccaa gacatacttt ccacctaaaa taattgttgc aacataagac
     3061 agaccgataa tcaaggtaat gaatggatca aataaggagt ctaaaaaatt gactcgtttg
     3121 tttttaataa ttgcatcttc aatcttttct tcaaaatccg ctagatcttc tttttcttgt
     3181 ccaaatgttt taatgacttt gatcccagtg atactttctt gtgtcttatc attgatacta
     3241 gaaaaagctt cttgcgaatc acgaaaagca tcatggagtt ttgaccccag cactcgtgaa
     3301 gtgacggcca gcaaaggcag aggaatcagc gcaatcagtg ttaagcgcca atcaataaac
     3361 aaaatcatcg caatgatcgt tgttccgccc gtaatcactg aatctgcaaa ggtcaagata
     3421 ccagctcctg ctacattctg gatcgcattc aaatcattcg ttgcgtgagc catcaaatcc
     3481 ccagtccgat atttttggta aaagagattg tccatttctg taaaatgttt gaataactgc
     3541 tgccgaagtt ctttctccaa tcgagctgca cttccccaaa tattcgtccg ccagatataa
     3601 cgaaaaagat attgtaaaaa agcagcgata atcaatagga agatccaccc gaaaatgatc
     3661 tttattgata aatttttatc ggcgatctca tcaatgatga tcccaatcac ttttggagga
     3721 actaactgta ccaaggccac tgcgaatagc gagaaaaccc caattaaata atgtttcttt
     3781 tcctgacgaa aaaaccagcc taattttcta aaaatcgaca ttttactcct cctttgtctt
     3841 tatcaagagc ctaggattgt ctgtgcttgt cttttttgct gaataatctg aatagtctaa
     3901 caaattacga aataaaaaac ggactgaaaa aatcagcccg taaaaagtcg ttgggtcaga
     3961 acctattttt tcccttggtt cttcatttgc tgcatcatct ggcgaatttt cttctcagat
     4021 ggtttttgcc ccattgacat catcattgat cgcaacatgt cctcattaac gggaggattt
     4081 ttttctaaat agtctttcat atatttacgc gctaggaaaa agccacctac tgcgccgatt
     4141 agcaaagcaa taatagcaat aagtacgaca atacctgttg ataacatata aatctctcct
     4201 ttctatttcc gaattgctct caatgactta ttttactaaa gtttattgaa aaaagaaagg
     4261 cgtattcttt agtttcatgc aaaaaatagt ggttttttta gaggagaaca aaatttcttt
     4321 cgtcatctag caagacgcgc catgaaaaaa aattaggcgt accgaatctt tttctttaat
     4381 tttttctttt tgaggtgtag aatgtaatcg tagataaata ggaaaggtgg ttttttcttg
     4441 aataatcaat ccaataaaca atttcataag catcgactta ttgaacatac aaatggttta
     4501 tctttagctg aagtaaatgg aacagttgat gtaccaaagg ggaaaagttt ttttaaaaca
     4561 cttcttgctt actctggacc tggtgcgtta gtagcagttg ggtatatgga cccgggtaac
     4621 tggtctactt cgatcaccgg aggacaaaat ttccaatatt tattgatgtc agttattttg
     4681 atgtctagtc tgattgctat gcttttacag tatatggcag caaaattagg aatcgtgagc
     4741 caaatggatt tggcacaggc aatccgagca cgcacaagta agacattagg tgtcatttta
     4801 tggatcttga ctgaattagc cattatggca acagatattg cagaagtcat tggtgcagca
     4861 atcgccctct acttactttt tgatattcca ttggtgattg ccgtctttat tactgtatta
     4921 gatgttttcg ttctcttact actgacaaaa gtcgggtttc gtaagatcga agcactggtt
     4981 gtctgcttaa ttttcgtcat tttattcgtt tttgtctatc aagttggctt gtctaaccca
     5041 gattggcaca gtttatttgc tggctttatc cctaacagtc atacattctc agactcaccg
     5101 gtcatcggcg gcgaaactcc gctgactgga gctttaggga ttattggtgc aacagtcatg
     5161 ccgcataact tatatcttca ttcagcagtt tcacaaactc ggaagatcga tcaccaaaac
     5221 gaagaagatg tagcacaagc tgtacgtttt tctacttggg attctaatat ccagttgtca
     5281 atggcttttc tagtcaatgc actgctattg atcatgggtg tcgctgtctt caaaaccggt
     5341 tctgtccaag atccttcctt ttttggtctg tatgaagctt tatctgatcc atcagctatg
     5401 agcaatggta ttttgattgc tgtagcaaaa tctggtatct tgtccacttt atttgctgta
     5461 gcacttcttg cttctggcca gaactctact attactggta cgttgaccgg tcaagtcatc
     5521 atggaaggat tcatccatat gcgtatcccg atttggctaa gaagactggt cacacgattg
     5581 ctttctgtga ttcctgtatt gatttgtgtt ttgtttacaa gtacaaaagg aacgatcgaa
     5641 gaacatgccg ctttgaataa cttaatgaat gaatcccagg tgttcttggc ttttgcgttg
     5701 ccgttttcga tgattccttt actgatgatg accaacagcc aagcggagat gggtagccga
     5761 tttaaaaatt cattcatcgt aaaacttctt ggctggttct ctgtgattag tctaacttac
     5821 ttgaacttac gaggattacc aggacagatc gaaagtttct tcggcgagca agccacttcc
     5881 gctcaactag cattggctga tcacatcgct tatgtaatca ttgttcttgt ccttctcctg
     5941 ttggcatgga cgattggtga tttatataaa ggaaacaaaa aatatgaaca gcttttgcag
     6001 gaaacggtag aagaaatcga ataaaaactg atttttccac acaaagaccc ttgatagtta
     6061 ggttaaaaat ctagctacca agggtctttt tatttagaag ataaagcttt tttcagattt
     6121 aatttactga aaactagcat gcttgttact acgatgaatt gcgttaatgg cacgcctgca
     6181 ccagtgtaga cacttaaaaa atagtccttt ttttctggcg cataagaaca aaaccatgag
     6241 acaccgaaaa gaaaaaccaa ttgtgtcagt attaatccgg tgaccacaat caaagaggaa
     6301 ctataatagg cacgatcagc ttttgcaaat acggttccta gtatcataag taatatccct
     6361 gctattgaag caatcagcac gggaatagcc agaacttggt aaatttttat gatgacattc
     6421 gcgacttttg ctgattttgt taattggaaa ttaggtgttc tcggttcatt actatcgatc
     6481 caattctcca ttagcggaag gtttaataat tcttgagcct gtttgacttg ttcttgcgtt
     6541 ccgaaactat aatttcctcc ttgatggaaa tctttatatc gccatgcata agcgtatcct
     6601 gattgaagga aatcgaatac gattggcaga tcactcatcg tctttccatc tcctgatccc
     6661 atcagataga actctttttt ctttttgatt gttcccttgt gataggcttc ttctaattct
     6721 tggttgactt ttttccaaaa atcattggtt tcttttgcat tgttttggta ataaccagat
     6781 tcgcttacca catctcgcaa tgcccaaaag atgatatctc cgggtaattc ttttactttt
     6841 ccaccttgag tccacgcgtg agtcgtatat aattcgtcaa tacgggtaga aacagtatgg
     6901 aatgtaggag atgcggctaa tgctttattt agtgcagcat gtgagaccca tatctcactg
     6961 ttttctttat tcaagttcgt ttcatcatct attcggatca gttgtttcat caaacgtcca
     7021 aattctccac tggaacggtc attgatggtt gcaataccgt atgttgtctc atttttatat
     7081 tcgatccatt gatagacgat ggaaaagcca actataggaa gtaaaaacaa tgaaagtttc
     7141 agccattccc gtttcgagag aagaaagagt cgttttttta acttctttac ttctttgaaa
     7201 gaaaagtctt gccctagcaa gatctggacg aaactgatac ccaaagctac tacagtaaaa
     7261 ggaagaagcc agatagaatc ttcgcgaatg aaccaaaaga aaggaaaagc gacagaaagc
     7321 cctaaaaacc atggcagcaa ttttctgcta gaaccgtatt tgcggtaata aatagctaat
     7381 aaacaagcga taatgatctc aacagccgga atcacgaggg cattacgata tgttcgagtg
     7441 gaatattcat gtgtaaaact aatcggagaa aacaaaaaat aaaaatagat acttaaaaga
     7501 agatacttgt tcttcacaat cggtttaaac gcgagtgcaa ccaatatact ggcaaaaata
     7561 ctgaacatgc cgaacaaaac tgcataagaa agatgcagtt tatgtgcaag tactaaaaat
     7621 aagctgtagg agattccttt ggaaagtgtt gtagtcgtat actcccctag ccatttaccg
     7681 tccaaaatat tttgagcata gcgaatcagc agctggtcat cataggttgc cgacatgtct
     7741 gttccataga ttgctttact atataaatac aaacgaataa ttgtaaataa tagggctatg
     7801 ccaagaataa agtatgtatg tttctttttt agttggttga tcaagggttt taaagtgttc
     7861 agcatctttt cgtccttcca atcacgtttt gccacttgct tgtgaaactt ttcgtttttt
     7921 tacttttcta ccagtatacc ttgtttgtct aaatttaaaa agagggggga gaagatgcat
     7981 gatgaaaaag actgcaaaaa gtactccttt gtttcttaaa caaaaaagga caaggcaatg
     8041 tgcctggtcc ttttttgctg ctgtcagata gaaaatctta gagtatcact tcttctgcta
     8101 ataactcagg aaacacttct ggatcgaatg tttgcgcttt gaacatggcg atttcttttc
     8161 catatggtgc gtaagctttc tttttatctt tgcctacata gggagtttcc aagattttcg
     8221 aaatattgtc aaacattgga tcatggacga tcttattcag cgtgtcgaat ccgattgttc
     8281 caaaaccaat attggcgtgc cggtctttat gcgaaccttg cggattttta gaatcattga
     8341 tatggagtac tttaagccga tctaacccga tgatcttatc aaactcttcc attacacctg
     8401 aaaagtcttc tttaatatta taaccggcat cattcgtatg acaggtgtca aaagtaacgg
     8461 ataatttttc attcaatttt actccatcga tgatttgcgc taattcctca aatgttcgac
     8521 cgatttccgt acctttacct gccattgtct ccaaagcgat ttgtgccgtc tgttcttttg
     8581 tcaaaacttc attcaagcct tcaataattc tagcaattcc ggcttctgtt cctgccccta
     8641 catgagcacc tgggtgaagt gtaatttgag tagcgcctaa agcttccgca cgaacaatct
     8701 cttctctcag aaattggacg gcaaaaggaa acatttctgg tttgatcgta ttccccaaat
     8761 taatgatata aggggcatga acgacgatat ttgttagacc tttttctgcc atgtacgctt
     8821 ttccggcatc aatattcatc tcttcgatgg cttttcttct agtgttttga ggggcgcctg
     8881 tatagatcat aaaagtcgaa gatccataac tggctgcttc ttctgctgct cctaacagca
     8941 ttttctttcc gctcatgctg acgtgtgagc ctaataacat ggatggattc ctccttgaaa
     9001 ggcaattgtt actaacaatt cctatatatt ggtaagatga tatacaaaga aaatgattga
     9061 tttttctttg ccctgttctc catagtaact gaaattttct ctaaagaaaa gcttaagaac
     9121 tcttttataa taaagatcat tttctcacgt tttaactctt ttaaaaaaga aattcctgcc
     9181 aaaaacagcc ttgtctgttt ttttgttcta ttatttgcta taattagcga aataaaacca
     9241 ttagttgagg tgaccctttt gccacgcaaa aatactcgaa aaaaacagaa tagaagaaaa
     9301 aaagaaaaat ggtttgttcc taagattatt tttagagttt ttcaatcatt gactgttttt
     9361 attactgttc ttcttttgat gttcgcagct ctaggtattg gaataggcgc tggctatttt
     9421 gcctatctcg tcgaagacac acaattgccg actaaaaaag cactgcagac agaactagga
     9481 aacattacag aaacgtcaaa gattgtttat gcagataata cagagatatc aaaaatccag
     9541 acagatctga tgcgcacgac tatttcatct gataagattt cacctttatt gaaaactgca
     9601 attatcagca cagaggatga atacttcgat aaacaccaag gttacgttcc taaagctgtt
     9661 ttacgagcat tagtatctga agctactggt attggctctt ctggtggatc aactttgaca
     9721 cagcaattag tcaaacagca gatccttaca gatgaaacaa cattcaaacg taaagcaaac
     9781 gagatcattc tttctgcaca agtagaaaaa tacttttcaa aagatgagat cattgctacg
     9841 tatttgaatg tctcaccatt tggtcgtaat aataagggac aaaacattgc tggagtccag
     9901 gaagctgcaa aaggaatctt tggtgtagat gcaaaagatg tgacgctgcc gcaagctgct
     9961 tatatcgctg gattacccca aagcccgatc acgtatagtc catacacgaa cacaggcgcg
    10021 ctaaaagaag atctttctgc cggccttgcc agaaaagatt ttgttttatt cagtatgtat
    10081 cgtgaaggcc aaattacgaa agaacaatac gaagaagcaa aagcctatga ccttacaaaa
    10141 gactttctac cacaacaaat cgcagaacag actgacagag aattcttgta ttatactgtg
    10201 atgaatgaag caacaagaat cattgcacaa caattggctg aaaaagacaa cgctgatatg
    10261 tctgattcat cggtcagtga cgcttattat caaaaggcac agcaaacgat ccaaaataaa
    10321 ggatatacaa tccattcgac aatcgataaa gatgtctatg ctgcattgca aaatggcgtt
    10381 gaaaattatg gatacatgtt agatgatggt gctggcagtc aagtagaaac cggaaatgta
    10441 ttgatggata acagaactgg ccgcatctac ggattcgttg gcggacggaa ttacagtcag
    10501 aatcaaaata accatgcctt cgatacagaa cgtcaagctg gctcatcaat caaaccagtt
    10561 cttgtttatg gacctgcaat cgacatgggg cttatcggat cagaatcccg tgtatcagat
    10621 tatgcaacaa cttggcaaga aggagaaaat gctggcgaga aaatcgtcaa tgctactaac
    10681 gaaggatcga acaccttcca aaccgtccgt gagtcactag aatggtccaa caatatcccg
    10741 gcttatcact tatatcaaga tgtgttgaat tccggtggat cgaaacaata tgcttacgag
    10801 cactacttgg caaagatgaa ctacccggca aatgacaatt ggggcgttga atctgctcct
    10861 ttaggtacag tagatgtcac gacgcttcaa cagaccaacg gatttcaggc tttagcaaac
    10921 ggcggcgtct atgaagaagg ctatatcatc gattccatca cagataacgc aggaaatgta
    10981 atttataaac acgaaagcaa ctctgtccgg atctattcgg aagcaacggc atcgatcatg
    11041 aatgacatga tgcgtagtgt gatcaatgca aaaatcacta caccattcaa agatgccatc
    11101 tcaagcttaa acggaaatct aggaaaagca gattgggtcg gaaaaactgg ttctaccaat
    11161 gaataccgag attcatggct aattgtctct actccgtcta ttaccatcag tagctgggca
    11221 gggcacgatg acaatactgg aatggacagc aaagcaagga tacgttcggc taattatctt
    11281 gccaatctga tcaatcaagc ttaccaagca aaacctgaca tttttggcac tgatgagaaa
    11341 ttcgaattat catctgatgt gatgaagaaa aaagtatctg gatttactgg tcaaacacct
    11401 ggaaaagtta ctgtcaataa aaaatccatc caaacaccag gaaaaacagt tgaatcgcta
    11461 tgggcgaaaa atggaccgaa gaaaagcgcc tttaaatttg gcgttggcgg taccgatgaa
    11521 aactatacag attactggaa caccgcttca gcttatgcaa gagcgaatca agcaactaaa
    11581 gaatcagatg agaaagatga ttgatttaat agaatacaca gta
//
LOCUS       DAFEOM010000078        11444 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.081, whole genome shotgun sequence.
ACCESSION   DAFEOM010000078
VERSION     DAFEOM010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.081"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            184..1182
                     /locus_tag="LP524_002341"
     CDS             184..1182
                     /locus_tag="LP524_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL2235361.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1294..1803
                     /locus_tag="LP524_002342"
     CDS             1294..1803
                     /locus_tag="LP524_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBL2235362.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1833..2492)
                     /locus_tag="LP524_002343"
     CDS             complement(1833..2492)
                     /locus_tag="LP524_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBL2235363.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2494..3258)
                     /locus_tag="LP524_002344"
     CDS             complement(2494..3258)
                     /locus_tag="LP524_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HBL2235364.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3266..3943)
                     /locus_tag="LP524_002345"
     CDS             complement(3266..3943)
                     /locus_tag="LP524_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HBL2235365.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3980..5365)
                     /locus_tag="LP524_002346"
     CDS             complement(3980..5365)
                     /locus_tag="LP524_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBL2235366.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5575..5659)
                     /gene="ffs"
                     /locus_tag="LP524_002347"
     ncRNA           complement(5575..5659)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="LP524_002347"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5682..6197)
                     /gene="tadA"
                     /locus_tag="LP524_002348"
     CDS             complement(5682..6197)
                     /gene="tadA"
                     /locus_tag="LP524_002348"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBL2235367.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6304..7161
                     /locus_tag="LP524_002349"
     CDS             6304..7161
                     /locus_tag="LP524_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2235368.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7242..7577)
                     /locus_tag="LP524_002350"
     CDS             complement(7242..7577)
                     /locus_tag="LP524_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235369.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7644..9014)
                     /locus_tag="LP524_002351"
     CDS             complement(7644..9014)
                     /locus_tag="LP524_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBL2235370.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9092..9628)
                     /locus_tag="LP524_002352"
     CDS             complement(9092..9628)
                     /locus_tag="LP524_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL2235371.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            9937..11418
                     /locus_tag="LP524_002353"
     CDS             9937..11418
                     /locus_tag="LP524_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2235372.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         3516 a         2301 c         1854 g         3773 t
ORIGIN      
        1 tttgcatact tcataataat aataacataa tacctaacct ctcgtaaaaa tactagattt
       61 gttttctttt atttttcatc actagataca ttctataact taccgtttcg atttcgaatt
      121 caaacatttt tgagaagcat tctcaattaa attctgctat agtgtgaaaa gggaggaatt
      181 tttatggaaa tctatgacat tacaatcatc ggtgcaggtc ctgtagggat gtttgccgct
      241 ttttacgcag gaatgcgcca agctaaaaca aaaatcattg acagtctacc tcaacttggt
      301 ggtcaattag caactttgta tcccgaaaaa tatatttacg atattcctgg gtatccagca
      361 ataaaagcaa gtgaattaat cgatcaatta gaaaaacaat taactacttt taaccataca
      421 ttccatttaa aagaagaagt tctctcgctt actcgtgaag acgaagttat cgagattact
      481 acaaataaag gcatccatta ttccaaagca gtcattttag ctttaggaaa tggttcattc
      541 cagccaagaa aattgaattt agataatgct gaatcatttg aaaaccacgg attggattat
      601 ttcgtgaatg atttgatgaa gtatgctgga aaaaaagtcg ctattgctgg aggtggtgat
      661 tctgctatcg attggacgtt gatgttagag cctattgcaa gcgaagttta cttgattcat
      721 cgtcgcccgg aatttcgtgc gcacgaacat agtgtatcac gcttgaaatc ttcatctgtc
      781 aacttgctta ctccctatct catcgatgga ttatcaggca ataatgggga actgacagat
      841 atccggttaa aaaaagtgaa aagtgacgag acgattgatt tgatgattga ttctctgatc
      901 gtcaattatg gattctcttc taatttagaa catctttctt cttggggact tgatagtacc
      961 agaaatgcca ttactgtaaa atcagatatg tctacttcta ttccaggcgt ttatgcagct
     1021 ggtgacatct gtacctatga aggaaaagtg aaactgattg ctactgggct cggtgaagca
     1081 cctactgcag tgaacaacgc tttacactat attaatccaa aggaacgaac tcagcctggt
     1141 cacagcacaa gcttatacga taaaaatctt cgtcctagtt gattttttat atctctttgc
     1201 atttcaagtt aaacccgaaa gcagtttctt gcattttttt catgttccgt taacatgaaa
     1261 tagagaaaga aaaaataaag ggggagcaat aatatggtcg tcaaaactga gactttagaa
     1321 gaaaaggcaa gagaactatt gaaggaacgt ggcgtaactg tagaagatat cgcacaactt
     1381 gtcatgtttt tacaaaaaga ctatatttct gatttgactt tggcggattg tactgaaagt
     1441 gtcaacagcg tgctaacaaa gcgtgaagta cataacgcca ttatcactgg aatccaatta
     1501 gatatattag cagaaaagaa aaaattgatg agtccgttgc aacatattat cgaagatgac
     1561 gaaggccttt acggtatcga tgagatccta gccttgtcaa tcgtgaatgt ttacggtacg
     1621 atcggcttta cgaattatgg ttatatcgac aaggtgaaac ctggtatttt aaaagaacta
     1681 aacagccacg atggcgttca tgtccatacc ttcttagatg atatcgtcgg agcgatcgcc
     1741 gctgctgctg ccagtcgatt agcacacaaa gaaccagaaa aaagaagtcg ccttactcaa
     1801 taattcaaca aaaaaagaaa gaaaacgtct atttagacga gttctttctt ttttgtttgt
     1861 ttcaagctat tttttcctaa gaaaagaaaa acagcaaaaa ataattctaa taatacaaaa
     1921 aataagataa agctatgcat gaaaaattct tctggaagta cattgatgat tggatcagta
     1981 acgggatcga ataaccagtc atcgttgttg aaaaacgttt catgaaaaag gataaaaaag
     2041 cgatcaaatc ctatcatcat gaaaaaaccg aaaacaactg gcagtaacat gccgatttga
     2101 aacggacgaa tcaaccgcca aagccgatca ttcttttgca aatatttgat aaacaaaata
     2161 ctcgggatca aagtgatgaa aaacacagca taatccacta aaaacaaaat tttcacttca
     2221 taaaagtgat gggctccgct agcagaaact gggaaatctg gcaagctgag gatggttttg
     2281 aagggattat tgagataatt cattaggtga tcaaaatttt tcagtaacgt ctcttgatct
     2341 aaagaagtat aatccaaaat ttgcaaatgt ccgatatcaa atacgtatag tggtcgaaag
     2401 ttgatcgtca caccaactgc cagactgatc agtgtcagaa ataggctgaa caatccagca
     2461 tattccatcc actgccatct cgaatttttc atattagaag ctccattcat ccagtgattg
     2521 tttcacatag gtagctggga taggcaaccc aggtacatct tcttcttttg taaaacctga
     2581 taaaacgagc aaagtatcta tattattgcg tatcccagct tggatatctg tttcatagtt
     2641 atctccaacc atgattactt cttctttttg cagccctaat acttcaacgg ctttatccat
     2701 aatgatggct tctggttttc cgatgtagat cggcggcgtt tgggttgctg tctctacaaa
     2761 agagatgact gaccctgctc caggaagaag gccgcgttca gtaggtatat tcttatctgg
     2821 gttcgttcca atgaaagtag cacctttttg aatagcaagt gttgctttaa ctactttttc
     2881 ataggtcaga taattatcta agcctaccac tacatagtca ggagcctctt cttcccatgt
     2941 aaatccggca gctaaaatca agtcaatcaa tcctgcttct ccgataacat agactttttt
     3001 cccttttcca tctgctttca taaaatcgat tgttgccaaa gtagctgtgt agactgtctc
     3061 tggagctaca tgaatgtcaa actcatctgc tagacgatta gctactgttt ctggtgactt
     3121 agtcgtgtta ttggtaacaa acaagaaagg taattttctt ttttgcaatt cttccacaaa
     3181 tcgtttaccg gccggtatgg gttgtgtccc acgataaatc gttccgtcta aatcaattaa
     3241 atatccttta tagtccattc atccactact cttccctttt tctgatcgta aatgctgttt
     3301 ttgttttttc tacttgttta ggttgttctt gtttttttac tttttcagag gtttttgctg
     3361 actctgtttt tcgattttta ataaccggtt ttttgcgatt agatgcgact ggtccttttt
     3421 tctcatcgat atacgcacga ttatggtttt ttttcttttt tcggcgctga gttgtctttt
     3481 cttttttacc gccgattctc tcgatgacaa aatacgcaca accaaaattg caatattcgt
     3541 aaagatagtc ttccaaggaa tcgattcgct gatctggatg cgcccgtcga ttatccgctt
     3601 ggaaaaatcc ttttaggcgt agttgttcat aaccccaatc tcctacgata taatcatatc
     3661 gggctagtac atcactgtaa cgttctccaa gcttttctgc attaaatcct tcacgataat
     3721 cggacacaag ccgataatgt ctttcaccta tcaaaaaatt actgtcatct aacagtgtca
     3781 ctagctcgcc tttgactttt tctgctttag gctcctcaac gatttcttcc aaagcagctg
     3841 tcagttcttc ggtgactgtt gtttcctgtt cagctgatga ttttttctta gcaatttctg
     3901 ttttatgctc aacgttctgc tgttcatttt gttttgcaga catgcgttca ccacctatct
     3961 tttttattat accttgtttt tatttgattg ggtagtgagc tttcaagtaa tctcctacca
     4021 cacttcggat aaattcaggg aacaaaaatt cattttttcc agcaatctca atcgttggaa
     4081 aaaatggaac aaacatcaaa tgatctacgg tagtaaaagt ataattttta ttcagatcga
     4141 ttggcttgtt cttccaaaag acttgatggt taatagaatc atatgtgatt ccattataga
     4201 ccatctgccc aaaaatctta ccgcgaaatc ccattccctt cataggatag ttacgtaaaa
     4261 agttacgatt tttttctatt tctaaaacta agcggatcag atcatttcca cccaatgtga
     4321 catttaacaa gtgcatcgga tgcggcaacg cagtgtgaag ctgatcctga ttgacccgcc
     4381 cttttggaat ttcagttaaa aacagaccac tgtttaatat tgctgcttcc gtctttcctc
     4441 gttctttcac cgcctcaaag gctacttgga taaacgaatg ctcattgaag atatctagac
     4501 tcagagaaaa tggcaaatct gctacttctt tttcttttaa taattcatgc ccacgttcta
     4561 gataactatt tatttcttgt tcatcttctg taaaagctgt cattgtttcg gtagggatgg
     4621 ctttggctga atgcttaagg atttttttct cttcatcgat ggttaaatgg acttcaccga
     4681 catactgccc gtactttcca gcggctgcta tttggacatc attgattacg tgaccttctc
     4741 tgaataaatg atgggtgtgc gatcctaaaa taacatcaat tgctggtatt tcttttgcaa
     4801 tttggaaatc atcctgaatc ccgagatgac tcatcaaaac taaaacatcc acttgtgaac
     4861 gtaaatcttc gactaattct ggcaaaatat cataaggatt ccgtatgtcc cagccattcg
     4921 gattataagt aagcggaaat ggagcagtaa atgcaattag tccgattttc gtctggtgtt
     4981 tcgttgtaat gattttatat ggtttcgtcc atgccggtgg ctggagcgta tttttatcga
     5041 ataagttatc caggatgata tcaaactcag catgatcgta taaatgattc aactcttctt
     5101 tggaatttcc tacgccttca ttgtttccta tcgtcgctgc atcataacca acttcattca
     5161 tcagcaagat attttcctgt ccattcgttg cttctgacaa tggatgccaa cgatccacaa
     5221 aatcacctag atctactgca atcgttgtcg ttttttcttt cttttcgttt tctcgtttac
     5281 gttgatccaa aaaccttctg atccttggcc aattttctaa atgagaatgc agatcattcg
     5341 tatgtaaaat cactattttt tccatgtacc ctctcgcctc tctttatctt tatcatacca
     5401 taggcagctt tttcttccat tgaaacaagc tctatttcag agtttttcct gccttgaagc
     5461 aaattgtata agtacaatat tcgataaatc agtctggcta aaaaatcagg aacattcatt
     5521 tttacagtac tataaaaata aaaaaaacag acccgaaagt ctgttgtcgt ttcattataa
     5581 tcagacacat ggcggaacct gcttagtgct gcttccttcc agacctgaca cgcttcacaa
     5641 gcccaccatt gtcctagcct ttcggcaaag aatagtatac cttatttttc attcgttgac
     5701 cagtcttttt ttaatttctt ttttcgctgg cggagctttc ggaaaaagtc agtcaaaatc
     5761 atcccacatt cgtcttctaa aatgccacct tctacatatg cttggtggtt aaaccgctcg
     5821 tcctccaaaa gattcatcaa ggtaccggct gttccgcctt ttggatcata cgcgccgaaa
     5881 taaacttctg ctacacgaga caaaatcatc gcgccgctgc acataggaca aggctctaag
     5941 gtaacaaaaa gtgcagcatc ttccaagcgc cagcttccca ctttttcaca ggcttccttg
     6001 attgcaagca tttctgcatg tgtagtagca tcttgcgtgg tttctctaag gttatatcct
     6061 cttccaatca cttttccatc caatacaacc actgctccaa tcggaacttc atgaagcatt
     6121 tctgctttct ttgcttctgc caatgcaagg cgcatccatt tttctttttc tactgcatca
     6181 agatttgtat ttaccaatta cccttccctg cctttgtttt ttcttgtttt actttagcat
     6241 atgataaaaa ggctgtaact aacttttttt tctatggtaa aataaataca agaggtgaac
     6301 aagatgaaaa tggataaatt acttgctctt tacccacaag cacagaaaaa aaactctcca
     6361 gcatcagaag cggatatcct ttcttttgca attgatgatg cttttatctg gatcaaacaa
     6421 gattcattat ctcaacaaga aaccagcctt ttgaaagcgt tattccctgt aatcaacgat
     6481 caaaaaaaac acccatggta tcgctatctt tttaaagatg cttcttgtgt tcaagaaaaa
     6541 agctttcgtt tgatccagct tcacgtcgaa tcaaaaggag aattcttaaa aaaagaatgg
     6601 caagatacta ttttggaatt atttcgctat tctgctgatt tcttttttta tacagacaca
     6661 gatgcgctct tagtcgaaca aataaaccgt gattatcttg atgctactga aatcaacggt
     6721 atttttctat ctttagatgc tgattttgat acgacaacgt ctgtattcgt tggttctttc
     6781 cattcacctg gcaccgacct cgtccctttg tttgctgaag aaagaaaaat ttttttaaca
     6841 gagcaaaaca atctttatac tcacagccgt actttttcta tgcaagatgt ggctttacat
     6901 tactatacaa aagatactgt aaaagatagt aagttgatca aaagttacat agaactgatc
     6961 aaacaaaatg aaatggaagt gatcattcaa gaattatgga atgaattagg caatgtcagc
     7021 tctgctgcaa aaacactgtt tcttcatcgt aatactctaa aatatcgaat cgaaaaattt
     7081 caagaacagt ctggtttcaa tctaaaaaaa gccaacgatt tactgttttg ctatctttta
     7141 ttgcttcatg aacataatta aagatagatt aaaggggttg tgacaataat tttgtcacaa
     7201 ccccttattt cgaatgaata gtgttcaaaa gctagtttct cttacatcat tttttcaaca
     7261 aaatgcatta gccggtcttt tacatcttcg gttgaatctt tgattgaatc accataaaca
     7321 gagattttct tttttcctga tttgattttt gctaacattc ccatcaaaga atccaagtca
     7381 ctatctgaaa gaccatctac gatttctagt aatttttcat ttccatcaaa tttgtcatag
     7441 acgaattttt ttactttcgt ccgagtagtc atatgatgga tcttttccat tacttttccc
     7501 gatacaacga ctgctgtcgc tacactggca gcggctgcta cgcttaatcc gattgttact
     7561 tttgttgatg ttttcataag tatcagctcc tttttatcta tctccatcat agcatagaca
     7621 aaaaaataaa cgaactaatc cactcagtga aatctcaatc ctttagggaa aaagttcttt
     7681 actgttcctt ttacttgttt cccaaatccg acaggtatct tatctaatac tagcaaaacc
     7741 catccttgat tcccatcacg ttgaaatgtt tctcctgctg tgtaagattg ccattccttt
     7801 tgagtgatcg gcaagcaagg tatattctct atcttttttg tagcaagcgc taatgcatag
     7861 ctaggttcaa atcgattctt tttgaaatct cctaaatgca aacctgtacg tacgactttt
     7921 aaaccatcga gagaaggggc taactcagga acttcccaaa gatggtcgtt gaacactaaa
     7981 agtcttccag tagcttcata atgaaaatca tttgaaaact ccgtccagag tttttcctgc
     8041 tcttttgtca tttgcacttt actttttttt cgtgtcttct tttctttatg catctgattt
     8101 tgtccatgaa aagtcagctt tgcaacaaaa tgtccttcac cctgatcttt atgaggccat
     8161 atacgtattg ttttttccaa accagctacg cttccccatt cagaacgtcc tgaactaacg
     8221 gattgagtca aaggaatctc ctcaatcgtt actggataat tttctacaag ccagctaatg
     8281 atctcttcat tttcttctgg tgcaaacgta caagtcgaat aaatcagttg tcctttattt
     8341 tttagcattt ttattgctga agacaaaatc tcttgctgac gtttttgaca gtatagcgga
     8401 gattcttctg tccactcttt tattgcattt ggatcttttc ggaacattcc ttctcctgaa
     8461 caaggagcat caaccacgat ccgatcaaaa aagcccgaaa aatgagggac aagttctgcg
     8521 ggtgcatgat ttgtcactat tgcgttcgaa actccccatc gttctatatt ttctgataaa
     8581 atcttcgcac gtttcggaaa gatctcattc gtcactaata accctttccc cttcatttga
     8641 gcagctaatt gggttgattt accaccggga gccgcacaca aatccaaaac tttttcaccg
     8701 ggttttgctg cagcagctgt tccaacgatc attgcacttg gttcttggct atattcatac
     8761 ccagcttgat ggaggaaaga ctttccattg acagtgccaa gaaatccctc attgctgtag
     8821 ggagcgggtt gtaattcttc gctatggtaa gtttggacca tgtccaatcc tgccggtttt
     8881 agtggattcc accgaaatcc ttttttcacg cttccttgtt caagcgcaga aaaaaaatcg
     8941 gatgcttctt cgcctaaaag aagacgatat tttttgataa attgctgtgg taacgttgtc
     9001 gcttcctcct tcattccaat gtttcgttag acattttaca atctttttta cgaaaaataa
     9061 aggtggctga tctacttttt catgttgctg cctaaggtga ctgttccttt aagatatttt
     9121 ccaagtattc atccgtcacc tcgtttttat cccaaacagc gttcatcatt tcaagaaaat
     9181 cttgagaaga atcatctgag cgcaaacttc ccaatgcatt aagccatacc aattcaagcg
     9241 acttttcttc tttgatcttc gtgtgacctt tgacatatga gaaaaatggc cgcttaaata
     9301 gcgaatccat gtctaaatca gattttgcaa tgatttcacg aatactttca tggatagaaa
     9361 aaaaatcttt caataagtag acaatgctta aaacatcatc ggaactgcat tcagctaagt
     9421 agttttcaaa ataaacagtg atttcttcta atatcttctc ttttaacccg ctgattgatt
     9481 tgaaatgtgt ataaatcggg tatgtagaac aaccgcagaa ctctgctaac tctcttgcag
     9541 taattgactt cattccatat tcttccacgt ataccatact tttgaacagc aaatattctt
     9601 tactataatt tgttactctc cccatcattc ttacctccgt ctcttttcat ctacccataa
     9661 ataaataaca taaataattc taaaaatatt attgataatt atcaaaacat atttagttat
     9721 atccattaaa aatcaatttt tccgccttaa aaacaagaat atcttgacaa aataccctag
     9781 gaaataaaca atagaataaa caaggtaaca aaaaaattaa ataacgttat ataaaaaaga
     9841 aggcgacact ctgtgagaat aacacagact gaaatgactt tccactcaca gtttttgtct
     9901 aactatatac aaaaacagaa taaaaaggag gtatttatgc taattgaagc aagaaaaaaa
     9961 gagttacttg ttctacaaat ggtcattcaa aaaaataatt gttctttaca agaattatcc
    10021 aatgacttac atattccaaa acggacaatc aaagaactga tccgaaaaat aaatgtatca
    10081 ctcaagcaat ttttattgat tgatgaattt atttattcta atcataaagg agagatccaa
    10141 accaaagaaa ccgatcaaac taaaaagtta gctgtttttt ctaagttaaa attatgctac
    10201 ctaaaagaat ccaatcgttt taactatttg atgttactaa tcaattatcc acaaactgct
    10261 gtaccaaaaa aatatttact tgaacaactt tatatatcgc cctcctatct cgaaaaactg
    10321 acaaaacagc tcaatcattc cttaaaaaaa ttccatttac ggatcctatc tgtaaaaaat
    10381 tgctgtactc ttgaaggaaa tgagttatct atccgtctct atctttactt ttttctttca
    10441 gatacatttg gaggtaacga ttggccattt tcctcacttg aattaaatgc actaaaaatg
    10501 aacagtacag atgagcaaat agaaaaaatt tcaaaacaag aaaaaaatat ctacttgctg
    10561 ttaactttat tcatattacg aaacaaacaa caggcatatt ttccaaaaac ttcttctgaa
    10621 atcactgaat tgatggactt attgcagcat acacatgatt attcagaata ctttacttct
    10681 ttttcagtga gtcgcccttg tgaagagaca gaaacagaga gagcctactt taattatttt
    10741 ttctattgtt tttttccaaa tcaaatctct tttcgacaaa aacaggagat tggtttttta
    10801 ttttccacaa gcaaaaacct tatttgtcga caagtcggtc aattgatgac ttacttaact
    10861 caacagtttc ctctcaacca tttagaaaat aaaataccaa tttattttta ttttcttgtg
    10921 ttggcagttg tttttttcga actgttgggt gaatccagca gtagtttttc tacactttgc
    10981 tttccagaag ccacgtccaa tcattctagt tcttttatac aggaaaagaa attaagagaa
    11041 aaaatcgata caattttttc aaaaaatggg gaaagaaacc aaatcagtca ataccttagt
    11101 caattgatcc aacacttact acaaacagaa acgaaagagc agttactcat ccatttgcag
    11161 ataaccaaac agtttacagg aaattatcat cttcaaagcc atttgcagca gttatttcgc
    11221 aaagatgctg ttaaaattac cgcatattca gaacatgctg atttgatcat tacagatcag
    11281 catgagaaaa tttcagatga aacggagact ttctatctgg atacaacgca caatacagtg
    11341 ttatttggca agttaataaa agcggttcat gaactgtata tgaaaaaagg ttatgagaac
    11401 aaaacaaaga ggatgtgaca aaattcttgt cacatcctct tatt
//
LOCUS       DAFEOM010000079        11413 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.082, whole genome shotgun sequence.
ACCESSION   DAFEOM010000079
VERSION     DAFEOM010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.082"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            257..532
                     /locus_tag="LP524_002354"
     CDS             257..532
                     /locus_tag="LP524_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235373.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            545..2623
                     /locus_tag="LP524_002355"
     CDS             545..2623
                     /locus_tag="LP524_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBL2235374.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2783..4669
                     /locus_tag="LP524_002356"
     CDS             2783..4669
                     /locus_tag="LP524_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL2235375.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4681..5628
                     /locus_tag="LP524_002357"
     CDS             4681..5628
                     /locus_tag="LP524_002357"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBL2235376.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5648..6175
                     /locus_tag="LP524_002358"
     CDS             5648..6175
                     /locus_tag="LP524_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL2235377.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6238..6891)
                     /locus_tag="LP524_002359"
     CDS             complement(6238..6891)
                     /locus_tag="LP524_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBL2235378.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7023..7865
                     /locus_tag="LP524_002360"
     CDS             7023..7865
                     /locus_tag="LP524_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBL2235379.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8023..8919
                     /locus_tag="LP524_002361"
     CDS             8023..8919
                     /locus_tag="LP524_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBL2235380.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8922..9635
                     /locus_tag="LP524_002362"
     CDS             8922..9635
                     /locus_tag="LP524_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HBL2235381.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9655..10173
                     /gene="msrA"
                     /locus_tag="LP524_002363"
     CDS             9655..10173
                     /gene="msrA"
                     /locus_tag="LP524_002363"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBL2235382.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10170..10397
                     /locus_tag="LP524_002364"
     CDS             10170..10397
                     /locus_tag="LP524_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HBL2235383.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10549..11100
                     /gene="lepB"
                     /locus_tag="LP524_002365"
     CDS             10549..11100
                     /gene="lepB"
                     /locus_tag="LP524_002365"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBL2235384.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11183..11365)
                     /locus_tag="LP524_002366"
     CDS             complement(11183..11365)
                     /locus_tag="LP524_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235385.1"
                     /translation="MIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVY
                     SFIKTLEARKVFKRAV"
BASE COUNT         3960 a         1929 c         2345 g         3179 t
ORIGIN      
        1 tttttgtcgc agccccattt ttttgttgtg ataaaagaac aagaaaggca ggaatgggaa
       61 agtaaacatc cttttgtcag aaacttttat ctttcttaag ttttacttga tatctgatac
      121 tatttggaat agaatgaggc gttgaaaaat ttatggaaat ttctaactag caataaacga
      181 tgaaacaatg aaatattttt catccaaaaa gaactttgct ataatgatgg aagtttgtgt
      241 ataggagatc tggaaaatga gagagagttt tgaacaacaa aaaaaactgt tgcatgatcg
      301 ctatggcgcc ctttcgatgg atgaccgccg tcaaatactt tgtaagttgc gtaaaagaaa
      361 tatattgatg tatcgtcagt tggaacgtct gaaacatgat ctgcttcgtt tggagtcaaa
      421 acgtgtacag tgtgaattag aggggaacca aacacaggtt gaagtagtcg aaaccaaaat
      481 tttaaagaaa aaagaacaat ttttaaaaat gcttacacaa aataaaaaat aaaggggatg
      541 gacgatggaa tttgctgagt tagttattgt atttgcagta acgatcacct tatcgaacat
      601 agctagccgc attttgccaa tgattccagc tccactgatc caaattttcc ttggagtggt
      661 attaggtctg acagagtggg gacaatcaat cgattttgaa cctgaattgt tcttagttat
      721 gattattgca ccattgttat ttagagaagg agaaaaagca gatatttctt cgattctcaa
      781 aaatttcggg acaattttgt tcttggcatt tggaggagtc attttgaccc ttgtcggggt
      841 aggtgcgacg ttatcattcc tgttgccaag tgtcccactt gctgcctgct ttgcttttgg
      901 agcagcgtta ggtccgacag atgcggtagc agtcagttct ctttctggac gagtgaatat
      961 ccctaaaaag gcgatgcata ttttggaagg agaaggtctt ttgaatgatg catctggtgt
     1021 tactgccttc cagtttgctc ttggtgcgct gatcaccgga tctttttcag cagtaaacgc
     1081 ggggatgagt ctagtagttt ctagtattgg gggagctttg atcggctttt tgcttgtctg
     1141 gttcaaacaa aaaataattc atttgatcga aaaagcatca gcacaagatg tcacagggta
     1201 tctgctgatc gaacttttgc ttccttttct agcctatgta ttggcagagg catttgatgt
     1261 atctggaatc attgctgcag tggcagcagg tattctccaa gcttctggtt ttaggaaaat
     1321 ctctgtgttt gatgcagaat tgtcgagctt gtcccacagt acatggacaa ctattgcttt
     1381 tacactgaat gcattagtat ttatattttt aggaatcgaa ttgacgcaag tgttctcgcc
     1441 agtatgggga gacggtttat atccaaacgg tcttttatta gcgattatcg tattaatctc
     1501 tgtcatgctt tttgtgatca gattcatttc tatcagtctt ttttatgtat tcaaagatgg
     1561 cagtaaaaag ttcaaaaaac agttgaacga gatcttgatc ttaacatttg gcggtgtaaa
     1621 gggaacagtc agtttggcta cgatttttat tttgccattt tcaatcaata atatgatgtt
     1681 ttaccagcgg tctttgcttt tgtttctgac ggcgggtgtc atcttagtga ctttggtcat
     1741 agggatcatt gtcttgccta tgctgacgga aacagaggaa gcgaaaagca cggatttgaa
     1801 tgctttgatg attttagaag aggtcgttga agttttgcga aaagaaataa aggaaattga
     1861 tcgtaataca aaagaatttt tagccactga agcagtgata gaaaattatc aagaacggat
     1921 acgtgatctg tacttggaag atttaaccga tgatgaaaag caagaagtac aagaaataca
     1981 agcactgatt ttgtcgatcg aacgggatgg attggatgaa agttatcgtt cgggcaagct
     2041 ttcttctaat ggttatcgtt tttactcccg ttttctttct cgattcgaac attctatcac
     2101 aagccagatt ttgtctttta tcggcttttg gttcattgtt gtccgtaggt taatgcgaat
     2161 cgttctccat ccgaaaatgt tttggcaaag aagatatgct gatcgtcaga catttattag
     2221 tgatgaagat atccaagaaa tcagagagac atatcaaaaa aatactcagt tgattattga
     2281 aagtttagat aacttgactg atatttatga tgaaacgatg gttcaatttt ttatccagca
     2341 acgcaaactt gaaggaataa aaatgaactc ggggaatttg atttcttcgt ggatgatcca
     2401 gcaagatagt ctgtttacga aaaaaatgct aagaggttat tatcttgaaa gaaaaatcat
     2461 tgatgaatac gaagttgctg aaaaaatcac gacgttttct gcgaacagct atcgcaggaa
     2521 tatcaatttg ttagaatctt atacgatgaa caaaccatct gataactttt ctttccgatt
     2581 tgcgtttcga gcaaagtcaa aaaaaacgag tccaataaaa taaaaaggta aaaattttca
     2641 agttttataa aaaacaaaac atatcagaac aggatcgaat cggtcctgtt ctttttttga
     2701 gaacacaaaa agaacaaata ccaaaaaaat tttcctgaag ttcactattt ttatgataca
     2761 atatccaaga taaggaggga acatgttgaa agaattaaaa gtaactgacc tcaaaaaaac
     2821 ttatggtgaa aaagatttgt ttgaccagat ttcttttttg gtccacgaaa aagaccgtat
     2881 cgggttgatt gggacgaacg ggacgggtaa aacaagtctc ttaaatatta ttgccggtat
     2941 cgatagcagc gacggtgatc ggcaaacagt gttttatcca actgattacc gtattggtta
     3001 tctttctcaa gcagctgaat tttccgatga attaactgtt ctccaagcag tctttcaagg
     3061 aaacagcccc ttgatccaaa ctgttcgagc atatgaggaa gcattgatcg aactagggga
     3121 aaacggagaa gaccctgatg tgcaaaaacg ttatgctaaa gcagaagaac aaatgaataa
     3181 agaagatgct tggactacag ataccaacgc aaagattatt ttgcaaaaat taggaatcag
     3241 tgaattagat aaaaaaatca gtactttatc aggtggtcag aaaaaacgag ttagtctggc
     3301 gcaagtgttg attgatgaac ccgatttact attactagac gaaccaacaa accatttaga
     3361 ttatgaagcg atcgagtggt tggaaaacta tttgaaacaa taccgtggcg cactattgat
     3421 ggttacccac gatcgttatt ttctagatag agtagcaaac cgaatcttcg aactttcatt
     3481 tggaaaatta tacgagtata aaggaaatta tgaagcttac gtcttagaaa aagcagaacg
     3541 tgatcgtgta gaagtagaac aagaagaaaa gcgcaagcgg ctttataaac aggaattagc
     3601 ttggatgagg gctggcgtac aggcacgtgg caccaagcag caagcacgga tcaaccgttt
     3661 cgaagatcta aaggagaact tgtaccaagt aaaccaagaa gatgatttag agctgaacct
     3721 agcgacacaa cgtttaggga aaaaagtact ggaaatcaaa gatggcagtt accgtataaa
     3781 tgaccaaaca ctgctagaac atttagattt attgattcaa tctcgggaaa gactaggtat
     3841 tactggtaaa aatggtgcgg gaaaatcgac attgcttaat attctagctg gcagaattcc
     3901 cctggatagc ggtacaatgt ctatcggtga aacggttcat cttgcgtatt atacacaaga
     3961 aaatgaagaa atggcaccag ataaacggat gattgcttac ttacaagaag ctgctgaaga
     4021 agcaaaaaca gcagatggca gtcaaatcgg agtagcagaa ttacttgaac gatttttatt
     4081 tccaagattt atgcatggga cactgatagg caaactttca ggcggagaaa aacgccggtt
     4141 atttttgttg aaactgctga ttcagcaacc taatgtttta ttattggatg aaccgaccaa
     4201 tgatttggac atagcaacat tgacgatttt ggaagattat tttagaagtt ttccaggtgc
     4261 tgtgatcact gtttcccatg accgttattt cttggacaag gtagcagata agttattagt
     4321 attccaagga aacggaaaac aagaacttta ttacggaaac atgtccagtt atttactgaa
     4381 gcaaaaagag acccagcagc ctgctgaaaa agcgaaacca aaaatacaga gcaaggaacc
     4441 tgctgaaaag aagaaattat cttacatgga acaaaaagaa tgggaaacga ttgaagatga
     4501 gattgcagaa cttgaagaaa aaatctcatt gctccaagaa gagatgaatc atcaaggaga
     4561 taatttcact cgattacaag aattgcaaaa cgacgtatct gaaacagaag cacaacttga
     4621 agaaaaaatg gcacggtggg aatacttgag tgaatgggta gaagattagg aggaaaaagg
     4681 atggaacaag cgtatcttga cttaggaaga aaattgttgg aagaagggca tcgtaaagaa
     4741 gacagaacag gcacaggcac attaagctta ttcggatacc aaatgcggtt tgatctgcaa
     4801 aaagggtttc ctttgttaac aacgaaacga gtaccttttg gattgatcaa aagcgaattg
     4861 ctttggtttt taaaaggaga tacgaatatc cgctatctat tacaaaataa aaaccatatt
     4921 tgggatgaat gggcatttga acgctacatc aaaagtccag actataatgg accagatatg
     4981 actgattttg gccatagatg tttgacagat cctgaattca atgaagaata taaaaagcag
     5041 agcaagcttt tttgcgagaa gatcctaacc gatgattcct ttgctgaaac atacggcgat
     5101 cttggacaca tctacggcta tcaatggcgt cactgggaaa caaaagacgg tggctttatc
     5161 gatcaaatca aacaagtgat cgaagcaatc aaaaagactc ctgattctag aagattgatc
     5221 gtttctgcat ggaatccgga agatgttcct agcatggctt tgcctccttg tcatacgatg
     5281 tttcaatttt atgttcaaga gggacggcta agctgccaat tgtatcaacg cagtggggat
     5341 gtgtttctag gcgttccgtt caacattgca agttatgcgt tgctgactca cctaattgca
     5401 catgaaacag gtttagcggt tggcgaattt gttcacacat taggagatgc tcatctttat
     5461 atcaatcata tcgaacaaat gaaagaacaa ttgagccgag aaatacgcgc atttccgact
     5521 ttagttttga atcaggaaaa gcagtcagta tttgattttg agatggaaga tatccaatta
     5581 gaaggatatg acccacatcc ggcgatcaaa gctcctatcg ctgtttagaa tcagaaagga
     5641 tgagaaaatg ttagctgcaa tttgggcaca ggatgaaaac ggattgattg gaaaagaaga
     5701 ccgtcttcct tggcgtttgc caaatgatct gaaattcttc aaacaaatga cagaagcaaa
     5761 tacactagtg atgggacgaa aaacattcga agggatgggt agccgtccgt tgccgaatcg
     5821 tcagacgatt gttttgacga gagattcttc ctataaagca gaaggcgtgc atgtcatgca
     5881 tagtgtggaa gacgtgcttg ctctggaaaa agaaacagac ggtatctttt tcatcgctgg
     5941 agggtcagct gtttatcaag aatttctgcc attttgtacg atattatacc gtacagtcat
     6001 ccatcatgta ttcgatggag atgcttattt tccacctgtt gactggtctg attggtcact
     6061 gatcaatact agtcaaggag aaatggatga aaaaaatcgt tatccgcatc aatttgaaac
     6121 gtatcagcgt aatgagaatc ctgtaaaaag cgggacaggc gaattgacga tataaaaaag
     6181 ctatggatag actagctgtt atttagagct gtcccatcca tagctttatt ttttttatta
     6241 agtaaagaat aaaacagaga agtacataaa acctgcacct aacataacaa acaaatgcca
     6301 aacaacatgc atatagcgga tagaattcaa gctataaaag aatgcgccta tcgtgtaaga
     6361 aagcccgcct gctaccatca aaataacacc agtcattcca agagagtgat aaagaggaac
     6421 agcagccacg acacagagcc accccataaa aatatagatg atcgtagata cttttgatac
     6481 tctctcccgt ttatgcaacg tcaacgcttt atatataatg ccaagaatcg caaacaacca
     6541 gattaataca aataaagtcc aacctagcca tcctcggata ctcagcaaac aaaaaggagt
     6601 ataacttcct gcaatcaaaa gaaaaatcga actatgatcg aatacttgga acaccttttt
     6661 tgcttttgta aaaatcaatg aatgaaacaa agtagacgtt aaaaataaaa gaatcatcat
     6721 cgatccatag atagcataag agacaatatg gagggaggaa tgcaagtggg caccttttac
     6781 taaaagaacc actagtcccg cgatactcaa tccagtccca attccatggg tgactgcatt
     6841 aaacacttca tttaatattt catacgttcg attaaatttc gttttttcca tgaaaacggc
     6901 tcctttacta ggtgtaatta atgaaaaatc attatttgtc tgttatacta aaaatagaaa
     6961 ttgtcttaca tgtagtttaa catgaataag gcatcttgtt aaagaaagag agagagaaaa
     7021 taatgacaaa tgtaaaaatc gtaacagatt cttcatgtac gatggaaaaa aatgtacgcg
     7081 atgctttggg cattcgtgtc atgccgctat ccatcatgat cgacggaaca gtatatcccg
     7141 atgatgacca actagacggc gaaagattta tggaaatgat ggcgaaagca aaaaatctgc
     7201 ccaaaacaag ccaaccacca attggagaat tcatctcgat gtatgatgag ctaggagaag
     7261 acggcagtga agtgatttcg atccatatga caaaaggctt gagtggaaca gtagaagcag
     7321 caagacaagc cagtaatcta acaaaaacga aagtaacggt tatcgacagt gataccactg
     7381 accaaggatt atcttttcag gtaattcgag cagctgaatt agcaaaagaa ggtaaaacag
     7441 tagagaaaat ccttgaagaa gtcaaaaaag tacaagacaa tacaaaatta tacatcggaa
     7501 tatccacact agataacttg gtaaaaggtg gaagaatcag ccgaacaact ggtttgctgt
     7561 ctagtgtttt gaacatacgc gtggtgatga atttcgccca ttctgaatta atccctgtaa
     7621 ctaaggggcg aggaaagaaa acgttcgata aatggtttaa taatttgaaa aatgaactga
     7681 aagaattgcc aaatgtgcga caaatcggta tttcacatgc ggatgcaaaa gaattggcgc
     7741 aagaattcaa agagggtttg caagaattat tcccggacat gcatatccct gtactgcata
     7801 cgaatccggt tatcgctacg catactggta aagacgcgtt tgcaatcatg tattatgtag
     7861 attagtcgaa aagaggataa gccctcagcc tgaaaggctt gcagcttatt ctctttttct
     7921 ttataaaatt aggaaaataa ttgttttctt ttttcataaa gactaccttt ttgataaaat
     7981 ttttgataac attagaacca atcgaataca agaggtgaaa aaatgaaatt aacgaaacga
     8041 catttgatcc tcgttttgtt cccattatta attgcgatca tcatctatgc aggtttagct
     8101 gcttctatac cgaaagctgg cccactaatg aaaccagtaa ctactgtgag agaagatcca
     8161 aataaaaaag aaaaacttca ctacatagct atcggagatt cgctgactga aggagtagga
     8221 gatcaaacca agcaaggagg attcgttccc cttgtcgcaa atgatctaaa agaccgttat
     8281 aatttgaccg cggttgaaac agaaaactat ggtgtaaatg gtgaacgaag tgaccagatc
     8341 ttaaagcgag taaaaacaaa agaagaaatt cgaaacaata tcaaaacagc tgattttatc
     8401 acactgactg ttggcggtaa tgatttgatg aaagttatcc aaaataattt attcgggtta
     8461 acggtcaatt cctttaaaaa gccactgaaa aactatcagg aaaatgtcac agaactattg
     8521 gaagaatttc gatccctcaa taaaaccgca cctatttaca ttttaggcat ctacaatcca
     8581 ttttatttga actttcctga gatcactgac atgcagacaa ttgttaacaa ttggaacaaa
     8641 ggaacagaaa cggtgacaaa aaaaatgccg aactgctact ttatcccaat caatgatttg
     8701 ctttatcaag gattgaatca ggaagtaggt gtcacagaag aaggaacaga tcaaacaact
     8761 agcacgacag aaaatctaaa tggcatcaaa aataatgtgc tctatgaaga agatcatttc
     8821 catccaaata atttaggtta tcaattgatg gcaaatgcag tgagagatca gctgatcgag
     8881 acacaaaacc aatggttaga gaaggaggat gatcaataaa catgaaaaca agatctgacg
     8941 tatcttcaaa caaaacaaag aaaacaaatt actggaaatt cgccttcctt atccttcttg
     9001 gggtagtact tggttcgacc gtatttttag gcagccgtat ttttgctaat cgggaaccag
     9061 aactcccaga aataccagct cttacggaaa gacaaggaga tcctgtctta acgatcaatt
     9121 caaataaaga aaaagtcaat caaatcatca gctttttcct tagtgagtat caaaaagata
     9181 gtgatattga atataaattt tatttggaaa atgaagcact actcaatggt actttcgaag
     9241 tactaggttt tcctatcgat ttctatttat attttgatcc gtatgtgatg gaaaacggaa
     9301 atgtccaact aaaagctaga agtctttcta tcggaacatt gagtttgcca atcaaagatg
     9361 tgatgaatat gatcaagaga aactataaat taccagattg gatcgaagtc aatacagatg
     9421 atctgactat catgcttagg ttggatcaat tccgaatgca aaacgggatg tatattaaag
     9481 cagataaaat cgacttagta aatgatgaca ttcggtttag tctatatttg ccaaaagata
     9541 cctctgatgc ttcaaacaac tcaaatacca acaaaactag caccagcagt actgaaagca
     9601 agacaaaaga aaataaaaaa tcatcggatg agtagtatta gagggggaat cataatggaa
     9661 cgagcaatat ttgcaggagg ctgcttctgg tgtatgatcc agcctttcga cacgcttcct
     9721 ggtatccata cagtgatgtc aggatatact ggagggcatg taccagatcc gacttatgaa
     9781 caagtaaaaa gccaaacgac tggacataca gaagcagtgg agattatcta tgatccaaca
     9841 ttgatttcat atgaacaatt agtagaactt tactggcagc aaactgatcc aactgacgct
     9901 ttcggacaat tcgaggatcg cggtgacaat tatcgcccgg tgatctttta tttgacagaa
     9961 gaacagcgga tcattgctga aaaaagtaag aaacagttag ctgaaagtgg acgtttcgaa
    10021 cagccaatcg ttacaaaaat cgaaccagcc caaccatttt atctagcaga agaagaccat
    10081 caagagtatt ataaaaagca tcctgaaaat tttaaaagaa accatgcacg ccgggctgcc
    10141 tttatcgaag aaaattggag gaacgaaggg tgaatcgaag cttttttcat tatttaatga
    10201 cactgcgagg cggtggcaca catgatgctt tatctgtttt cgctacagaa gccggtaaag
    10261 acatccaatt tcctaagcac agctctagct acgaagaaat ttctaattat ctggaactga
    10321 atgtcgacta tttgccgtcc atggatatct ttgacgaagc atgggaaaaa tatttagaaa
    10381 ataatcatga catataagag atagttttgt actttatgat caaaaaagtt ttttgtccaa
    10441 tagcctaatt gaaagaatta tttttatttc agctatctag tcgcattttt ttgatatttt
    10501 tcttataata aagaaagaat gaaaaaaaga gggatggatt gatagagatt gacaaaaaaa
    10561 caacgctata ttaatcgctt ctggcttgta ttcaaatact tacttgtttc tgtattcctt
    10621 gcgttcatgt tgcggggatt tttattcatc ccggtaccag tggaaggcaa ttcaatggaa
    10681 aacgtgttga agcaaggtga tatggttgtg atggaaaaat tttcggagat cagacgattt
    10741 gatattgttg tttttcaatt agcagatggc acgatttata tcaaacgagt aatcggtctt
    10801 ccaggagaaa atgtcagcta ccaaaatgat caattgaaga tcaatgggaa agtagttaag
    10861 gaaccttatc taactaaaaa cctgaaatca gatcatgcaa atgcatccta tacgacggac
    10921 tttacactac aagagctaac gggacaatcc aagcttccgg aagataatta ttttgtactt
    10981 ggagataatc gaagagtttc aaaagatagt cgttcgtttg gcacgatcaa taaaactgat
    11041 attctaggta aagcaagatt tgtctattat ccacttgatg aaataaaatg gatccagtga
    11101 gaaacaataa agcgtgcagt ctgttgagac tgcacgcttt attgtttaat ccaaaatcag
    11161 tttactacct tagtacgtaa aatcaaacag ctcgtttaaa cacttttcta gcctctaaag
    11221 ttttgataaa tgaataaaca accattattt gaatagggat ttggatcata ttcctgatga
    11281 atctacctag aaaaattact ttgaaatctg caccaaacaa aacagacatc agccaagaat
    11341 tgataaaaag gctgactggg atcataatca ataattgggc agcgatgacg gaaggcaggt
    11401 ttatcttttt atg
//
LOCUS       DAFEOM010000080        11364 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.083, whole genome shotgun sequence.
ACCESSION   DAFEOM010000080
VERSION     DAFEOM010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.083"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(51..560)
                     /locus_tag="LP524_002367"
     CDS             complement(51..560)
                     /locus_tag="LP524_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235386.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDVSPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(550..1275)
                     /locus_tag="LP524_002368"
     CDS             complement(550..1275)
                     /locus_tag="LP524_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235387.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(1498..3618)
                     /locus_tag="LP524_002369"
     CDS             complement(1498..3618)
                     /locus_tag="LP524_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBL2235388.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            3848..5026
                     /locus_tag="LP524_002370"
     CDS             3848..5026
                     /locus_tag="LP524_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HBL2235389.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            5042..5800
                     /gene="aroD"
                     /locus_tag="LP524_002371"
     CDS             5042..5800
                     /gene="aroD"
                     /locus_tag="LP524_002371"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBL2235390.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            5823..6308
                     /gene="ybaK"
                     /locus_tag="LP524_002372"
     CDS             5823..6308
                     /gene="ybaK"
                     /locus_tag="LP524_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBL2235391.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(6379..7632)
                     /locus_tag="LP524_002373"
     CDS             complement(6379..7632)
                     /locus_tag="LP524_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL2235392.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            7749..9098
                     /gene="gdhA"
                     /locus_tag="LP524_002374"
     CDS             7749..9098
                     /gene="gdhA"
                     /locus_tag="LP524_002374"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBL2235393.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            9210..10556
                     /locus_tag="LP524_002375"
     CDS             9210..10556
                     /locus_tag="LP524_002375"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBL2235394.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(10864..11250)
                     /locus_tag="LP524_002376"
     CDS             complement(10864..11250)
                     /locus_tag="LP524_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBL2235395.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3694 a         2041 c         2305 g         3324 t
ORIGIN      
        1 atcatgaatc gatcttctat accgcttgtt tttactataa aatgattgat ttagcccgcg
       61 ggctctaaac gatcaatata aggactttga gcaatagtag catcatgagt aaagagtaag
      121 ataccttgat tttcttcatg agctaaataa gaaagcaagt ggagtagctc ttggatctcc
      181 aatacactaa gttcatcaaa tgtatcttca ataacaatat tgtttcgctt ggaaagcaag
      241 gcatgaagta attgcagctt tactttttca aaagacgtca actcagctgc cgcacaatta
      301 agcaaggaag gagagacatc aagtttttct tgataggcca aacgatcttg tttgatttct
      361 ttttccggta catccaacag gacatggtct ttaagattca gataaggaaa catcggccaa
      421 tttttttgga tgatcgcaaa attatccagt tttgcaacaa gaacctcttt tttcaagtgc
      481 tccaatattt tatgctgttt ttcgagagga cacaaaataa cggtcaagtg ttcagcatga
      541 atcaaatatt caggtttcat tacgtccagg cctcctactt tgcaaatgat ggataaaaaa
      601 agtgaaaggg aatagactaa gaatgagaca gcatccactg agcccaataa gagtttgggc
      661 aaatccctta agtatatctg tgaaaaaaag atcattttgg aaatcgatgg taaaaaaagt
      721 ctgttgatcc atcggaattg taagtccgct taccgaagtc aagatatcac ggatatctgg
      781 cagttcagta gtctttaaga caagaaaatt gatcctttga agaagcgctt cataaactga
      841 ctggaagctt acgatccaca ataaaaccaa gatacaacaa agaaagagag gaaagaacag
      901 ctgccagaaa ataaaaagga ataatttggt tttagaaaga ccagtcaggt gccaattgat
      961 caattcctgc ttgttttgcc tgactgaata gtaaaagaaa aagataaaaa ggaaggcaaa
     1021 tagaaaaaat aaactgaata tgccttttat ataaaatcgg ctagctaatt ctaaagccgg
     1081 aagaagctct gtagatgcag aagcgccaat aaatgaaagc cgatcttgga cttgattggc
     1141 taaagccgat tgcaaatggt aaagagtcag gcatgctatc aataaaaaag cagaaaagct
     1201 tgaaaataaa caatagagta ttgtctgctt tttgtaataa attaatcgag cagtagcata
     1261 gcggattatt ttcatacttt tcacctctat aaaaagtata gaaaatgaat atgaataaag
     1321 tatgaaattc ctttagtgaa atcacgaatt aaaaccagaa aatattttgt aaaaagcaag
     1381 aggttgtgaa gaagctgccc tattctatta ccgtagatgc aagcttttga acactgttta
     1441 ttcgaaaaga ggagcagcga caataatttt gtcgctgctc cttttaaatt tattttatca
     1501 ttcggaagaa cttgtggtcg ttccgtgaag ttcttggtac gtttgcgttt tataaagatc
     1561 aacagttgat tgtccgttgt gttcactaaa gacactagtt gacttgtcac caagagattt
     1621 ttcagcttct tttaatttct ctaagccatt cttgtagtca taatcactgg tatcaattgg
     1681 gtctaatcca ctattcgtat agaaccgaag aagatcacca ttattgattt ggtcagaaac
     1741 agctaactgt ttatcaactt tttctttcag tgaatcaatt tcattctgta cttcctcagt
     1801 aggttcagta atagcttcac cagtttcgtt actatagatc gtaccaccgt agtaagtata
     1861 ttttggtgta acaaaatcac catcacggaa agaaacaagc tgttcatgat ctttcgagaa
     1921 tagatcttgt cctaactgaa tataattttt tgtatctaca cctagtaagt gaagtaatgt
     1981 aggcaaggca tcaacttgtc cgccgtaagt gtgattgatt ccgccctgtg tttgtccagg
     2041 gatatggatc atataaggga cacgctgcat ttgtgcattg tcgtagtttg tccaagtaga
     2101 acttgttttt ccaactaatt cagctagatt tttatttctt gaattcgaga caccgtaatg
     2161 gtcaccgtaa aggacgatga ctgaattatc atacaagccg ctttctttca aataattgaa
     2221 gaattcttgt acggcggtat ctaaataatt ggctgtcgca aagtaaccgt tgatcgtttc
     2281 atcatttgta ttggctaacg ggaatcctgc ttcgtcattg gtgaattttg aataaggata
     2341 atggttagat acagcaatga attttgaata aaatggttgt tgcagacgtt ccaagtattg
     2401 tacggattgt tggaaaaatg gtttatcatg caacccgtat tggaacgaat tgtcatcatt
     2461 aacatcataa taagaagaat caaagaaata atcatatcct aaattcttgt atgtctcgtt
     2521 tcgattccag aatgtaccag cgttcccatg gaataccgca ctaatgtaac cttgtgtttg
     2581 acttaaaata tcaggtgcag cttggaaagt attttttccg cctaattgtg tgaacaagga
     2641 gccttgattc aagccaaaca atgaattttc catcagcgtt tccgcatcgc ttgtttttcc
     2701 tgcttttact tgatggaaaa agttgtcaaa actgaacgtg ctgttgctgt ggtaaagact
     2761 attgatgaaa ggtgtaactt catgctggag accattctca tctgtcaatt tataatcgat
     2821 caggaattgc tggaagcttt ctaaatggat ataaatcaca ttttttcctc tagcgagccc
     2881 gaacaaatcg tcatttggtg ctgcataatg ttcttttaca taattttcta cttctttcat
     2941 atcattggga cttgcttctg cccgcacttg gtttgtctgg taagtttgaa caccgtcata
     3001 aaccatgaaa gcattcaagc caagatattt gaccaaataa tcacgagaga atgttcgagt
     3061 cagcaattct ggacgatcag cttccgccat aaaaagattt cctgagaaaa tcataactgc
     3121 taatgtagag atcgcaaaag ccattcttgc tcggatcggg cgagggtcca acttgatttt
     3181 ttttgcaatc agcagaacag tcaagataat aaaatcgatc caatagatca catcatatgg
     3241 gcgaaacagt cgaatggcac tttctcccag accactcgct actttcccag cacctaaaat
     3301 cgtgttgatc gtaatgaagt ccgtgaattc tcgataataa gtgacattcg aaaaaacaag
     3361 caatgtcaaa agaaaataaa tgaccatcat cgtgatataa gcaactctag ggcgtctgat
     3421 ataaagagca attccaagta ataacaatgt agttgcaata gggttgataa acaagataaa
     3481 gtattgaatg ccactttcga ttccgagatg gaaatcaact aaataggcaa aaatcgtttt
     3541 caaccaaagt aaaacgacta gtaaagaaaa aaatccgagc ctcttattca aaaaggcagg
     3601 tgtttttata catttcaaga taatcttcct ttctgcactt cttaatcaca gaagtgtcct
     3661 gaaaccatgt tacacagctc acttattctc atttatttta ctagtatgac aatatagtgt
     3721 caattgaaac cagaagttta tgctgtttca ttaaggtttt ctttaagctt catgaaaaaa
     3781 tgagcagtaa gacaagtcct atgttttttg ttatacttgg gaagagaaag ctaaagcagg
     3841 tgaaggaatg aaagtaaaag taaaaaaaag agcaagcaat cgattgaaaa aaggatatcc
     3901 actgatccaa aaagaagacc ttgctcatcc ccaaaaagaa accacgcaat gggtggaatt
     3961 agttgattcg caaaaccaat ttatcgcaaa aggatatcta ggaaagcaga ataagggaat
     4021 cggttgggta ttgagccagc agaacatccc atttgatcaa tctttttttg aagaattttt
     4081 tgtgaagtca aaagaaaaaa gaactttctt tttttcagat gattctacta ccgcttttcg
     4141 tatattcaat ggagaaggag acgggatcgg cggtctgatt gttgattatt atgcaggtta
     4201 tgctgttttt tcttggtata acgaaacgtt atatgcacat cgtgaattat tgattcaagc
     4261 ttttcgtgaa atctacccgg aaatcatagg aggctatgag aaaattcgat ttgaatcagt
     4321 aaatctgcct gagagccagt tcatttacgg aaaagaggca ccggaaccct tgatcgtaaa
     4381 agaaaacgga gtaaaatttg ccgtttattt aaatgaagga ttaatgactg ggatcttttt
     4441 agaccaaaaa gaagtacggg gatcattagc agaaggtttc gcttctggga aaacagtttt
     4501 gaacatgttt agttataccg gcgccttttc tgttgctgcc gcaatgggag gggcaatcga
     4561 aacaacaagt gtcgatctag caaaacgcag tttaaaaaaa acacaggaac agtttgaagt
     4621 gaatggtttg gatgtcgatc agcaaaaaat cattgttatg gacgtattcg agtatttcaa
     4681 atacgccaaa agaaaacaat ttttatatga catgattgta ttggatccac caagttttgc
     4741 cagaaataag aaaaaagtct ttcgcgtagc aaaagattat ggggaactgg tcaaggatag
     4801 tctggatatt ttagcagaag acgggatact tattgcgtct acaaatgctg cgaatgtctc
     4861 gatagaacaa ttccaaacaa tgattgaaga ggaattccat gccaaaaatg tttcttttga
     4921 acaacttgct ttatatcgtc tgccaaaaga tttccagacg acggatacct ttttagaagg
     4981 caattatctg aaagtgtttg tttatcaaat taaaaataac cattagaagg agcgatgcag
     5041 aatggttcgg gtaaagggat tgaatatagg aagcggtaga ccaaagattt gtgcgcctct
     5101 tatcggggcc gtccgagagg aagtattacg tgaagcagtt cttgcacgtg aagcaggggc
     5161 cgatttagtc gaatggcgag tcgatcatta tcgtgaagtt ttttcttttg atgaagtagc
     5221 agaaacattg gttcttatcc atgagatgct tgatggactg ccgttattat ttacgtttcg
     5281 taccttagct gaaggcggag aacaagacat atcgatccgt cagtatcgcg acctttatga
     5341 acatgtgata cacacaggat tagtagatat ggtcgatatc gagttgttca aagtagaaag
     5401 tctaggaaaa agactgatag aggaaatcaa gtctttaaac attcctttaa ttatcagtag
     5461 tcatgatttt aaagaaacac cagcagaccc tgtcttgctt taccgattga atgttatgga
     5521 acattttgga gccgatatcg gaaaactggc agtaactccc aataatgaac gagatgtttt
     5581 gcggttgatg gagttgacta gacgggcaaa tgcttttgtc tccatgccta ttattacgat
     5641 gtccatggga aatcttggta aaatttctcg tctggttggc ggaacgacgg gttccgttat
     5701 gacatttggt tccttaagca gcgattctac ttctgctcca ggacaattac cggtcaaagc
     5761 attgaagcaa attattcaat tattaaatga accgttgtag aacaagtttg aggtgatatt
     5821 ttttggcaaa gaaaaaaata gtaaaaacga atgcaatccg tatggtcgaa caaaaaaaga
     5881 ttccctatac agagcacgaa tatgagtggg acgaaagcca cctaagtgcg tcatcggtcg
     5941 ctgaacagct tccagaaagc caatctcgta tatttaaaac actggttgct gtgggcaatg
     6001 tgacggggcc tcttgttgct gtgataccgg gagaagccga attgaatcta aaaaaattag
     6061 caaaagtttc agggaataaa aaagtagaaa tgctccattt aaaagatttg gaagcaacga
     6121 ccggatacat acgtggtggc tgctcgccta taggtatgaa aaaactattt cctacttatt
     6181 tagatcaaat tgcagaaagc tatgaacaaa tcattgtctc ggctggcaga agagggctac
     6241 agatggagtt agcacctcaa gatatctgtg cattaacgag cggacaattt gcagatataa
     6301 aacaataaat catacgtata aaagaaagaa ggaggccggg atgttgtttg tccagtctcc
     6361 ttctttcttt tttatacatt atgctctttg cccggcattc attcctgcta catgccctgt
     6421 agcgaaagcc gctgtgatat taaagccgcc agtataacca ttgacatcca gtaattctcc
     6481 cgcaaaatac agaccatcaa tgattttact ctccaatgtt ttaggataaa tttcctttag
     6541 agaaacccct ccgccagtta caaatgattt ctcaatagga aaagttttgt tgattgatag
     6601 cttaaactct ttgcatagtt gaacaaaatc ttgaatttgt ttttgagagg cttgttgtcc
     6661 tgtcagatga tccatttcaa gccgctcaag aaagaaaact aacaatcgct caggcaatat
     6721 accatgccaa gcattgacca agttcttttt ggttgcttta cttttttctg tcaatatatg
     6781 aataagctct tgtttggttt gtgtagggaa acaatccaga gaaagtgtca ctggttctcc
     6841 cgttttctct aattctttat tgacaaaact tgaacaacgc agtgcagccg gaccagaaat
     6901 accgaaatgg gtgaaaagta aatccatcgt atgttcagtc aatacacgac cttttttatt
     6961 taaaacgcgt aaagtgatgt cttgtaaaga aagcccttga agtgttttct cctgaatata
     7021 cggttcatcc gatatcaagg gggactctgt cgcataaaga ggcgtcaccg tgtgacccac
     7081 tcgcttcacg atcttgtacc catctccagt agaaccagta gatggataag tccgtccacc
     7141 agttgttaag ataatggcag gagcatggaa ctcttcgaat tccgtgcgta taccgtaaat
     7201 tttatgttct ttgtggatca atttctctac tcgagtagag aatagaagcg tcacatctag
     7261 ttctttcaga cgattgagca atgcttcgat aatcgttttt gatttatttg ttacaggaaa
     7321 catccgtcca tgatcctctt cttttaaatg tacgccttgt gattcaaaga agttcatgat
     7381 atcgaaatta ttccattgcg caaacgtact atataaaaac tttccatttc caggaatatg
     7441 tgcaatcaaa tcatcaacag gacgattgtt cgtaacattg caccgaccac cacctgtcat
     7501 cagtagtttt tttcctgctt ttttgttttt ttcgatcaag agtacgcgtg cgccttgttc
     7561 agctgcactg atcgctgcca tcatcccgct tgttccagcg ccgactacga tcacatcata
     7621 cagtttttcc attccctcac ctcaaggaaa gtgtagcata tctgaagaaa gaaaacagaa
     7681 aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga gggaagataa gcaaaggagc
     7741 gtggaacgat gacaaacgca acagaatatg ttcaagcaat tcaagaaaag ctacatcaaa
     7801 aggatcaagg gcaagaagaa tttttacaag caatcgatga attcatgccc acggttatgt
     7861 cttttttgga cactcatccc gaatatatcg aaaagaatat cttagaactt ttaacggaac
     7921 cagaaagagt tattcaattt cgagtgcctt ggcaagatga tgaaggaaac tggcgagtaa
     7981 atagagggta ccgtatccaa tacaactcag ctatcggccc ttataaaggt ggattgcgct
     8041 tccatcctag tgtcaatcta agtatcttga aatttttggc ttttgaacag attttcaaga
     8101 atagtttaac cggtctgcca atcggtggag gaaaaggcgg aagtgacttc gatcctaaag
     8161 gaaaatctga taatgagatc atgcgttttt gtcaaagttt catgctggaa ttgtcaaaac
     8221 atatcggccc atcgattgat gttcctgcgg gagatatcgg tgtaggagct agagaaatcg
     8281 gctatttgtt cggcgaatat aaacgattga aaaaatatga tactggtgtg ttgacaggta
     8341 aacctttgga tttttgggga agtaagatac gtacggaagc tactggttat ggattagtgt
     8401 attatgtcaa acatctattg aacgaagaaa aagattcatt taatcaaaaa acagtttttg
     8461 tttccggaag cgggaatgta gccatttatg caatcgaaaa agtgcaagag ctaggcggga
     8521 aagttgttac atgttcagat tctagcgggt acatctatga tccagaagga atagatgtga
     8581 aactattgaa agaagtcaaa gaaatcaata gacaacgttt gacggaatac gcaaaccaac
     8641 gtccttctgc tatttatcat gctggagaat ctgtttggac attgaaagaa aaagcggata
     8701 tcgctttgcc atgtgctact caaaatgaaa tagacgaaag cttggccgaa atcttagtag
     8761 aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc atgtacagta aaagctgttt
     8821 ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa agctgctaat gctggaggag
     8881 tagcagtatc tgctttagaa atgagtcaga acgtccagcg attgacttgg gaaagagagc
     8941 aagtggatga gcagctggat caaatcatgg aaacgattta ccatacgtgt cgccaaacag
     9001 cagccgagta cggcgacgaa aaaaatctgc ttcttggagc aaatgttgct ggatttgaaa
     9061 aagttgccaa agcaatggca gcagaaggtt tagtttgaaa aatgaaactt tcttggaaat
     9121 attgtcagca ttttgtttat ttgcttgaaa aataataaaa aaaaacgtaa agtattacct
     9181 gtagagtaaa cctataagga ggagaacaaa tggcacacat tcattttgac tattcaaaag
     9241 tagcaccgtt tgtcaacgaa caagagttag ggtacatgca aagccaagta acagcagccc
     9301 acaacgaatt acgggaaggt actggcgcag gaagcgactt ccgcggctgg atcgatttac
     9361 caacgaacta tgacaaagaa gaatttgcac gtatcaaaga agcagctaaa aaaatccaat
     9421 ctgattctga agtattagtt gttatcggta tcggtggttc ttatttaggc gctcgtgcag
     9481 cgatcgactt cttgaatcac acattcttca atttattaga tagcaacaca agaaaagcac
     9541 cacaaatctt ctttgccgga aattcaatca gctcaacata tattgctgac ttgatcgaag
     9601 taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa atctggaaca actactgaac
     9661 cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa gaaatatgga caagaagaag
     9721 caaacaaacg tatctacgct acaacagata aagcaaaagg tgcagttaaa gtagaagcag
     9781 atgcagaagg ttgggaaact ttcgtgattc ctgatgacat cggcggacgt ttctctgtat
     9841 taactgctgt tggtttgtta ccaatcgctg ttagtggtgc agatatcgat gcattgatgc
     9901 aaggtgccgc tgatgcaagc aaagcatact caagcgacaa actagaagaa aacgaagctt
     9961 accaatacgc agcaatgcgc aacattcttt accgtaaagg caaagtaact gagttgttga
    10021 tcaactatga accaggaatg caatacttct cagaatggtg gaaacaattg tatggtgaat
    10081 ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc aaacttctca actgatttgc
    10141 actctttagg acaattcatc caagaaggac gtcgcaatat ttttgaaaca gttgtgaagg
    10201 ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc agaagattta gacggtctag
    10261 gctacttgca aggaaaagaa gtggacttcg taaatacaaa agcatttgaa ggaacattgc
    10321 ttgctcatac agacggtgac gtgcctaact tgctagtcaa aattccagaa atggatgctt
    10381 atacattggg ttatacaatg tacttctttg aaattgctgt aggtatttca ggttacttga
    10441 atggaatcaa cccattcgac caaccaggtg tagaagctta caagaaaaac atgttcgcac
    10501 ttctaggcaa accaggcttt gaagatcttg ctaaagaatt gaacgaacgc ctgtaagata
    10561 agggtttttc gctagattta aataatcagt gatagaaaca tgttcgcttt aaaatgaaaa
    10621 ctggtaccca tttaggtact caaacatact gcatcgttta ttcagaataa ggggctgtga
    10681 caagacgttt gtcacagtcc cttgtttgta tagaaatcga ttgattgcct tgctctttag
    10741 tttgttttag gactttaaga acagtgaggc tgacttcgaa ggttaattct gtttttttgt
    10801 tttctagaat cagtaagcaa aaaagcactc acttcgagtg cttttttgcg atatattctt
    10861 agcttaagat aggcctaatt tttcttttac agatgagggt accttatcgc tggtaattac
    10921 ttttttctct atgatccgct tttcgcttgc tttcaactgc aagatcgttc cttcggggta
    10981 agcagaacca ttggcagagt caccagaagt aatgacatcg aaatctaact gtcttttttc
    11041 accgttttca gcataagcat ctacgttgta tttaaaacct cttccaatta cttccccatt
    11101 atctgctttg atatcagttt ctgcaggcat tggagctttg actaccgcat aataatcttt
    11161 acctacataa gtagactgat agtagtccca acctttccat cctataaaag caatcaatat
    11221 gataacgatc aaccctaaaa tttttttcat gaaagatctc cttttttatt tttattatac
    11281 ccgtggtgct agttaatttt aatatcctaa agaatatctc aaagttcctc caaaacgtcg
    11341 ttgtgctcga cttaatagaa cact
//
LOCUS       DAFEOM010000081        11075 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.084, whole genome shotgun sequence.
ACCESSION   DAFEOM010000081
VERSION     DAFEOM010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.084"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(<1..1539)
                     /locus_tag="LP524_002377"
     CDS             complement(<1..1539)
                     /locus_tag="LP524_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBL2235396.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQ"
     gene            complement(1532..2983)
                     /locus_tag="LP524_002378"
     CDS             complement(1532..2983)
                     /locus_tag="LP524_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL2235397.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(2997..4013)
                     /locus_tag="LP524_002379"
     CDS             complement(2997..4013)
                     /locus_tag="LP524_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL2235398.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            4233..5321
                     /locus_tag="LP524_002380"
     CDS             4233..5321
                     /locus_tag="LP524_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HBL2235399.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            5375..6901
                     /locus_tag="LP524_002381"
     CDS             5375..6901
                     /locus_tag="LP524_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBL2235400.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            6903..8108
                     /locus_tag="LP524_002382"
     CDS             6903..8108
                     /locus_tag="LP524_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL2235401.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            8365..9342
                     /gene="guaC"
                     /locus_tag="LP524_002383"
     CDS             8365..9342
                     /gene="guaC"
                     /locus_tag="LP524_002383"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBL2235402.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            9606..11030
                     /locus_tag="LP524_002384"
     CDS             9606..11030
                     /locus_tag="LP524_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBL2235403.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
BASE COUNT         3498 a         1868 c         2415 g         3294 t
ORIGIN      
        1 ctgtgtccga tattcttttg gagaaatccc ttgttttttc ttaaataaat aactgaaata
       61 gttagggtca ttataaccaa tttcaaaagc aatatctgaa atacgatagt ctgattgctg
      121 caataatttt tttgctaatt cgattctttg ttctgtcaga taatcgatga atgtttggtt
      181 aaacgattga gaaaaaatcg tactgaaatg agagggactt aatgcaactt gttgagcgac
      241 ccaagttaag gacatatctg gatcggtaaa attttctttg ataaaatcca atgcatggtt
      301 caaaattggc tgatatttgt tccttgaagg ctgtgagggc agagcagtta agaaagaaat
      361 gaacatataa actgtcgaac ggtaaagcga gtcgtcggcg ctgattgcta agcgttgact
      421 ggtggatgtg caatgaacca tttcttgcgg gattttcttt tctttcaggc agacatgctg
      481 gagtagctta gtcaagacaa ataaacgata taatcgtgtt cgagttggtg tgccttgtgg
      541 tttcgtcaag tgttcgatgt acttagatac ttcgtttttc gataatgaag acaaatcaag
      601 tgagaggtca aagtaaaaga cagaatcaac tggaagtttc gcttcttgaa tcaaatcctc
      661 atagctgata atttgttctt taagggaact ggcggtttca agtatcatct gcgtgatctc
      721 aaaagatgca ggtaattcgc tgattctttc tgcgacagga ccaatggcga cagaaaaatt
      781 atttttttgt cccttttcta attcatggac caacgtttgg gctacctgat aactggtctc
      841 gatcacattt tcttcaagag aatcaactat taaaaatttg atgaatcgag aggaaacagc
      901 agagaagatc acttgttgat tctccctaaa taaaaacttc aaataatcac tgagatgagc
      961 gtaatcttca aagtctgtac tgacttggtt tgctgctaat aatacggtca tttttttccc
     1021 tgctaaagag cgattgaaag attcagcaag tttcaatgtt tcactgatcg taagtgagcc
     1081 tttgaacaaa ccgttcaaga aatgattttt tttcaattcg aaaacaagat cagaagaagt
     1141 gtgcgtttct ttttgctgca aatctaaagc agttactgct ttttctattg cttgtttcaa
     1201 ttcgctactt ttaatcggtt taagcagata atcatctgcc tgtacctgga ttgctccttt
     1261 catataatcg aaatcgtcga atccactaat aaatatgaga cgagtccaag gaagtagttt
     1321 ccttgcttct tttgaaaaag tcaacccgtc catgaaaggc atttggatat cagtcagaat
     1381 gatgtcgggc tgtaagtcga taattgaaga cagtgccatc tcaccatctc cagcttcgcc
     1441 gacataagtg attggataat aagaaccaag gacagttatt tgttcccgca agttattgcg
     1501 aatgaggtat tcgtcttcga tgataaaaac tttatacatg tttgtttcct ccaattgttt
     1561 tgggagatag agggatcatc agtgtgacag ttgttccttc tcggtatttg ctgtcaatcg
     1621 tgataggttt ttgatcattt ccgtaataaa gaaggagacg cttgtttacg ttgtaaagtc
     1681 cgtagccgat actgaaatct gaatcgatcc ctttggctaa ctctttacgt atttctgcca
     1741 gtttttctgc agggatgcca atcccgttgt ctataacttt gaattggatc atctcatcaa
     1801 ttttgcatgt ggtaatacga ataaatcctt tgtgacggac gtacttcgtt ccatggtata
     1861 cagcattttc aatcaatggc tgcaagatca tttttaaaat agaaacagga aaaagtgttt
     1921 ctggtatatc gatcttatag tcaagcatat ggccgtatct tatttttaag atgactagat
     1981 aatcattgat atggctaatt tctttttcaa gaggaatcca gtcttgtcct ttgcttaaag
     2041 acgtacgaaa aaagccggaa agtgcatggg tcatagtcag agcttcttca aagcagtctt
     2101 gttcgatcaa agaaataatg gcatccaaac tgttataaat aaaatgggga gtaatctgag
     2161 cttgcaatac acgaacttca ctttgtgcta aatggtattg tttcaaagcg ttctcccgta
     2221 aagactggtt tagatcttcc gccattttat tcatgctagc ggtaagtgtt tgtaattcca
     2281 gtgtttccgg cagggttaaa cgaaagccaa ggttccctga agaaagttgt tctgacatct
     2341 cgatcagctg attgatcggt tcttggacct ttctctctaa aaagcgacga ttctttttcg
     2401 catagaaaaa aatggtacta agaactccaa gttccactat tgttaataga atgagcgagc
     2461 ggacaagtgt cgtatttttt ttgcttgcta agtcgatctc cacacgaaca aaatcttgta
     2521 agatatctga cagcagttga gtgacagaat ctacctgatt gatgatctct tcattggtat
     2581 ccataaaatc ttttgccaag atattattga tcagctcgtt ttgataatct tccagtgtat
     2641 cgatcgtacg tagcgataca tctaacgtac taatttcttt aagtgtattt gtattatttt
     2701 tgatctctcg aatatcattc ctaagttctt ctaggatatt ttcctcattg aactcatcta
     2761 cggttacttg gccagaaatc aagtcccaca tactttccaa aactttttga tcaaccttca
     2821 aagcaattgc atctgctttt tggatatttg cgatcgtctg ttgataaatc aaaagctgtt
     2881 gcgaatataa cagtatgctc agtagcaaag ggataacagc aaatagaagc atggctttgt
     2941 ttgcttgggc aaaaatctgt tttatgctta aattttttgt catgatactt ctccagtcaa
     3001 taatttctgg ttgggatgtt agctatattc tgctgggaaa agacagtctc ggaaataaaa
     3061 atatctttag gtaatgtttt tcctgtttga atatttccaa aataacgagt aacggtattg
     3121 gctacatacc agccgacatc aggtttacac tcgactacac aattgacgat tcctgagcgt
     3181 agcttctcga tcatatcttc ttgtccatca atagttacaa tgatcaaatc ttctccaggt
     3241 ttgattgatg tttcttttat cgcagctaaa gcaccgtgag tcatttcatc actatggctg
     3301 tataatacat cgatttcgga taaatcattt gtttctaaat atcgtttcat ctggtctttt
     3361 cctttcatcc gaataaaatc accgacaatt cggtgaacaa tagtcatcgg aggtttatgg
     3421 tcgatggttc catctttttt tagggttggt ttacgattga ttgattccaa aaatccttcg
     3481 gagcgtagct tggtcggtgt ggagttctct gaaccagcaa gttctaggat tctgatttcc
     3541 tgtttctttg aatttgtgaa gtgattgctg acaaataaac ccgcccgatt tccttctgct
     3601 ttgaaactgg ggccaatgtg ggtgacgaaa agctcgggat cattcgtttg aatatgtctg
     3661 tcgacaagta tgacagggat ccctgctcgc tttgcttctt ttaatacaga gtcccacccg
     3721 gattcttgca aaggagtaaa aatgatcata tccactttat aggcaatgaa cgtccgtata
     3781 tcttggattt gccgttcctg attcatgtag ccatttcggt agagaacttg gtaatcttct
     3841 ttttcaagcg cagtgataat tgattcagta tgctttttcc gccagctact ctccgttcct
     3901 gattgcgaaa aaccaacgac tagtgggtca ggtgcttcct ttgtattagg tgtacacccc
     3961 atcatttggt aagcgattac aacaagggct aaaaaaaata aaacacgttt cactttaaat
     4021 cactccatct ggatttgtta gtactatctt atcagataaa agggaaaatc aaagtcaaaa
     4081 ggaatcaatc gaaaaaaatc caagaaactt aaaaaaatcc aagacaaaaa aagtacacct
     4141 aaaaaattta aagtaaatcc cataaaaaaa tcaatgttaa cgctttcttt attagctata
     4201 ctcggtgtat caattgaaat gggggataaa aaatgaaaaa caaatttaga aagattgttg
     4261 ggactatcgc tattttgacc gcaagcattc tattaagtgc gtgtggcaat tcaggatctg
     4321 cagatgatag tacggggtat gtaggaatcg ctatgccgac caaatcagca gaaagatgga
     4381 tcgctgatgg gaacaacatg gtctcggaat tagaaaaact gggttataaa acagatcttc
     4441 aatatggaga agataaagta gaaaatcaag tagcacaaat agaaaatatg attacaaaag
     4501 gggtagatac acttgtgatt gcttctatcg atggttcggc tttaactgat gtgttagcta
     4561 aagcaaaaga agcagatatc aaggtgatcg cctatgatcg tttactgatg aattcagaaa
     4621 atgtcgatta ttacgcgact tttgataatt ttggagtagg tgtatcgcaa gcagcttata
     4681 ttgaagagca cttaggattg aaagaaggaa aaggaccatt cacgattgaa ttatttggtg
     4741 gctcgcctga cgacaacaac gcactaatca attacaacgg agtgatgtct gttttacaac
     4801 catatatgga caacggtcag ttggtcgtgc catctggaca gacgagtttc agtcaaatcg
     4861 ctacgcttcg ctgggatggt tctacggcgc aagcaaggat ggataatctg ttaagcgcaa
     4921 attatactga ccaaacattg gatgctgttt tgtcgccata tgatccaata agtttaggaa
     4981 tcatttcgtc attaaaaggc gtggggtatg ggagtgaaag taaaccttta cctgtgatta
     5041 ctggacaaga tgcgacagtt gctggcgtta aatcgatcat tgccggggaa caaacacaaa
     5101 cgatctttaa agacacacgt atcttagcaa aaaatacgat tgaaatgatc aaagcaatca
     5161 gtgatggaga agaggttccg gtcaatgata ctgaaactta tgataatggg gtgaaaactg
     5221 tccctacgta tttagcaaat acagtatctg tcgataaaga taattatcaa gcagagctga
     5281 tcgacaccga ttattataaa gaatccgatc tgaaaaatta agaggaacca aacaaggaac
     5341 taaagagatc taagtcagag agaggataag aattatggcg gattgtattt taaaaatggc
     5401 agatatcacc aaaaaatttt ctggaatccg agcgttagat tgcgtgaatc tgatgattga
     5461 acgtggggaa atccatgcat tgtgcggaga aaacggagcg ggaaaatcaa cattgatgaa
     5521 tgtcttgagc ggcttgtatc catatggctc ctatgacggg gaaatctttt atgatggaaa
     5581 gctgtgtcaa tttaaagatt tgaaagatag tgaagaaaaa ggcatcgtca tcattcatca
     5641 agaattagca ctaagtcctt atctttcaat aaaagagaat atttttctcg gaaatgaaca
     5701 agcagaaaga ggcgtgattg attgggaact gacagaaaag aaaacatatg aactgctgaa
     5761 taaagtcggt ttgtctatcg acccgaatct tctagtatcc cagattggcg tagggcaaca
     5821 gcaactagtt gaaatagcta aagcattatc aaaatccgtc cgtctgttga ttttggatga
     5881 gcctaccgct gcattgaatg aagaagaaag cgcgaatttg ctgagattga tccgagagtt
     5941 gaaagagcaa ggtgtaacct ccatcattat ttcacataag ctgaatgaaa tcgtggatat
     6001 cgcagatcga atcaccattt tacgggatgg acagacgatt gagacactag aaaatgaaat
     6061 gatcagcgaa gagcatatta ttcgcggaat ggtgggcaga gatttagttg atcgttatcc
     6121 tgaaagaaaa ccggatattg gagaagttta ttttaaagta gaagactgga cggtctatca
     6181 ccctcttgat cacgagcgaa tcataaacga ccacatcaac ttgcatttga aaaaaggaga
     6241 gattgtagga atagctggac tgatgggagc gggcagaact gaattcgcta tgagtctatt
     6301 tggacattct tatggaagca agatatccgg acgtatatta aaggaagggg cagagatatc
     6361 agttaagacc gttccttcag ccattgcaaa cggactcgct tatgtctcgg aggaccgaaa
     6421 atctttagga ctgaatctgc tgatggatat tcgggaaaac gtgacgctga gcagtttgga
     6481 taaaataagt caaaaaggag tattagataa agaaaaagaa gtaactgagg cagaaaattt
     6541 ccggaaaaag atgcgtataa aaacaaactc catccatcaa attgtcagta gtcttagtgg
     6601 tgggaatcaa caaaaagtcg ttttatccaa gtggctgatg acacaacccg atattctttt
     6661 tttggatgag ccaacccgtg ggatagatgt tggggcaaaa tatgagatct acacgattat
     6721 cgaagaaatg gcagcaaatg gcaaagctgt ctgtcttatc tcttctgaac ttcctgaaat
     6781 attagggatg tgtgatcgaa tctatacgat gaacgaagga cgtattacag gagaagtctc
     6841 gcgagaagat gcagatcagg aagtcttgat gaaattaatg acgaaagaag aggtgggtta
     6901 agttggaaca ggcaaaaaat cttacggata aaaaagagaa aaaactaaat ttgaaaagta
     6961 gagcagctga tctttttggt cgctacagca tggtgatcat tttagcggga ctacttattg
     7021 cgtttcaatt gatgacggat ggtattttct ggcgtccatt gaatataact aaccttgttc
     7081 tgcaaaatag ccatatatta gtactagctg cagggatgtt attagttgtg ctcttaggtc
     7141 atgtggattt gtcggtaggg tcagtgatgg cgtttgttgg tgcaattgcg ggcatgatga
     7201 tggtcaatcg ccaaatgaac ccgtggatga cagttatcgc atgtcttgca gtaggcgggc
     7261 tgatcggcgc atggcaaggt ttttgggtag cttatgcagg gataccggcg tttatcgtga
     7321 ctttagcagg attgctgatg tttcgtggac tgacacaagt tgttctagga ggccaatctc
     7381 tagcaccttt tccagctgtt ttccaaaaaa tgtctacggg ttatttacca gacttaagcg
     7441 aaggaagcag ccttcacttc ttgacattga ttttaggaat gatcatcacg atcgtgttag
     7501 ttgttggtca atggcgaaca agaaatcgtc ggaaaaatca tttatttgaa gtggaatctc
     7561 caacagcttt cttggtaaag gcggtattta cagcagtcgt tgtactagga ataacttata
     7621 tctttgcctc ttatcaaggg tatccgatta ttttgatcat tcttggtttg atcgttgcag
     7681 catatgggtt tttgacaaat aaaacagtag caggtcgaca gatttatgcg acgggcggaa
     7741 atcgaaaagc ggctgaacta tcagggatca aaacaaagcg aatcacgttc tgggtattcg
     7801 tcaacatggg agtcatggca gctttagctg gattagtttt agctgctcga ttgaatgcgg
     7861 caacacctca agcaggtaca tcgatggaac tggatgcaat ggcggcagta tattttggtg
     7921 gtgcttcgac ttccggcggc attggaacaa tcatgggtgc gattgtcggt ggcttggtca
     7981 tgggtgtatt gaacaatggc atgtcgattt taggcgtcgg ggtagactgg cagcaggcaa
     8041 tcaaaggatt gatcttgctt ttagccgttg tgctagactt gtataataag aaaaaacgaa
     8101 caacgtagga gaacgccggt tattcagaaa aaagactgtg acaagacatt tgtcacagtc
     8161 tttttttcgc taaaagcgaa cattcattgt ttcgtttgta tagactaaaa ataatatatg
     8221 tttttttacc attcgtataa attctctttt tgctcttgat ttttttgctt aattgagtta
     8281 aattacatag ggcgctaaaa aaagccttga ttaaattaat aatgttcgtg ttttgctgga
     8341 tgtgaacatc aggaggataa tgtaatgaaa gtatttgatt acgaagatat tcaattaatt
     8401 cctaataaat gtatcgtaaa tagccgttct gaatgtgata caacggtcac attaggtaaa
     8461 catacattca aaatgccagt agtgcctgcc aacatgcaga cgattattga tgaaacaatc
     8521 gcagaattct tagcggaaaa tggttatttc tatatcatgc atcggtttga tgaagcagct
     8581 cgaattccgt ttatcaaaaa aatgaaaaag cgtggattga ttacctcgat cagtgttggc
     8641 gtcaagaaag aggaatattc ctttatagag aagttggcag aagaatcgct aaatcctgat
     8701 tatatcacta tcgacatcgc gcatggacat gctaactcag ttattgatat gattcagcat
     8761 attaaaaaat atttgcctga aacttttgtg attgccggta atgttggtac accggaagcc
     8821 gtccgtgaat tggaaaatgc gggcgcagat gcaacaaaag tcgggattgg tccaggaaaa
     8881 gtatgtatta caaaaatcaa gacaggcttt gggactggag gctggcaact ggcagctctt
     8941 agatggtgtg cgaaagcagc tcgcaagccg atcatcgctg acggtgggat cagaacacat
     9001 ggcgatattg caaaatctgt ccgttttggt gcgacaatgg tcatgattgg ttcattgttt
     9061 gccggtcacg aggaatctcc tggcgaaaca aaagtcgaaa atggaatcgt gtataaagaa
     9121 tactttggtt ctgcttcaga attccaaaaa ggcgaaaaac gaaatgttga aggaaagaaa
     9181 atctggatcc aacacaaagg atctttgaaa gatacactag tggaaatgca acaagacctt
     9241 caatcatcta tttcttatgc tggaggaaga gatctggaag ccattcgaaa agttgactat
     9301 gtcatcgtga aaaattcgat ttttaatgga gacacaatct aaaaaataaa taaaatatta
     9361 ggatgttgtg aaaaagttgt cctgcattaa gtaataagaa cagccaaaca aaaatagttc
     9421 tttatatgtt ttgtttggct ggaattatta tcgcagatac aagcttttga acatcattat
     9481 tcagaataag ggactgtgat aagatttttg tcacagtccc ttatgttttg caaaaaaagt
     9541 ttttcttttt tctcgtaaat tcggtaaaat caaaagaaaa aatagacaat agaaaagaga
     9601 agcttatgtg ggaaaaagta gagaaaaaac aaggaccgat ttatcgtcaa attatggaac
     9661 agattatgag agaaatcgaa aacggaagga tcaatcctgg aagtcaactt gaatcagaac
     9721 gaaaactagg gcaacttttt ggtgtgaatc gttccaccgt tgtccatgca ttggaagagc
     9781 ttagggagct tggggtacta acgagcaaac gtggcagcgg atggtttgtt aatcagactg
     9841 aatgggggaa attcgctgtt ccaagaatcg attggcggca aatgatctct cctcgttatg
     9901 agcagacgga ctggtatgag caaaaaataa gagaagcaaa gcagatccaa aaaacatcgt
     9961 ttcttgattt atacgctagc gagatgccag atgagctttt gccgaatttt gagtttcctt
    10021 cttattctct tgaagaaatc ttagcggaag aaaaagaaat ggatctttta ggctatactc
    10081 cgttacgtaa caaaatcaaa cggtatttgg aaagtaaatt tgaattggca ttttccccca
    10141 accagcttct tgtgactggt ggcggtcagc aagcaatctt tttgatttta caaacgattc
    10201 tttcagctgg agaggggata gctgtagagt caccttcttt tttttaccga ttacctcttt
    10261 tcaaagcttc aggaacgcgt ttatttggga taccgatgga tgaagaaggg atcgacttag
    10321 aaaaactgaa agaatcgatc cataaaaata aattgaaagc agtgcttatc aacccgaatt
    10381 ttcaaaaccc tacaggaaaa gtgatgagtc aaaaacgacg agaaaagcta gtagaattgt
    10441 gtcgacagta tcggcttcca attattgaag acgatgtatt tacggatctt tcatttctaa
    10501 accaagaaga agtccttcct atacgaacga ttgatccgga taatgtgtta tacgtcggtt
    10561 cgttatctag ggttttagga aaaacaacga aaatcggttg ggtgatagga cctgagactt
    10621 ttatctcgca actagctcaa gcgcaagaaa tgatggagtt ttcgatgaat atccttacac
    10681 aaatcacagt ggctaatgta tttaatgaag caattgattt gaaaatgcaa gatgtaagaa
    10741 aaaagttgaa agaaaaaagc cgactgataa gcgcttgggg agattcacaa tcgtttttca
    10801 agcttcattc aattttaggt ggttactatg cctggattac ttgggatggg aaaaaactag
    10861 accccgaact agcagaaaaa gtagtagata tgggtcttgg aattgcacca agtctacttt
    10921 ttgggcaaga tataaatgga atcagaatca attacagtag gctggatgaa aaccaaatac
    10981 cgttgtttta ttcaaagatg aaaattctag aaaagtggtt aacagaataa tttcacaaag
    11041 taaatagatt aaagaatata acagcttgga tttaa
//
LOCUS       DAFEOM010000082        10960 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.085, whole genome shotgun sequence.
ACCESSION   DAFEOM010000082
VERSION     DAFEOM010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.085"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(40..1416)
                     /locus_tag="LP524_002385"
     CDS             complement(40..1416)
                     /locus_tag="LP524_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBL2235404.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(1544..2662)
                     /locus_tag="LP524_002386"
     CDS             complement(1544..2662)
                     /locus_tag="LP524_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL2235405.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            2804..3238
                     /locus_tag="LP524_002387"
     CDS             2804..3238
                     /locus_tag="LP524_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2235406.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(3316..3849)
                     /locus_tag="LP524_002388"
     CDS             complement(3316..3849)
                     /locus_tag="LP524_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HBL2235407.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(3943..5121)
                     /gene="mutY"
                     /locus_tag="LP524_002389"
     CDS             complement(3943..5121)
                     /gene="mutY"
                     /locus_tag="LP524_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBL2235408.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(5114..5911)
                     /gene="recX"
                     /locus_tag="LP524_002390"
     CDS             complement(5114..5911)
                     /gene="recX"
                     /locus_tag="LP524_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBL2235409.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            6018..7400
                     /gene="rlmD"
                     /locus_tag="LP524_002391"
     CDS             6018..7400
                     /gene="rlmD"
                     /locus_tag="LP524_002391"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBL2235410.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(7574..8152)
                     /locus_tag="LP524_002392"
     CDS             complement(7574..8152)
                     /locus_tag="LP524_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HBL2235411.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            8474..8944
                     /locus_tag="LP524_002393"
     CDS             8474..8944
                     /locus_tag="LP524_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBL2235412.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(9052..10278)
                     /locus_tag="LP524_002394"
     CDS             complement(9052..10278)
                     /locus_tag="LP524_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBL2235413.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         3271 a         2187 c         1838 g         3664 t
ORIGIN      
        1 tttattcaag caacacgtct ttcataacca agtttccaat tatcttcttg gctcgtcatc
       61 ttcgaattct tttctgaagt atcttcgttc ttcttcaggt tccgcttctg tttcttccgg
      121 atcatgaaaa atgacacgga ttttaaggat tcgagaacct tccatttctt cagaaaccag
      181 cgtaatatta tcgacatcaa acgataactt ttcgccttca tccggaatca tgcctaatgc
      241 tgtaatcaaa taaccagcca tcgtatctac atcactcata tgcagatcac tgtcaaaagc
      301 ttcattgaac tcatcaatca gcatacgtcc ttggatgata tattcgtgtt catcgatttt
      361 ttcataaaga ttctcaactt catctgattc atcatcgatt tctccgacga tttcttctaa
      421 aaggtcttct agtgttgcta acccaacgac gccgccatat tcatctaata aaatagccat
      481 ctgattctgc gtctttttca tttcatataa cagatcatca ataaagaccg tttcaggaac
      541 gaataaaggc tcctgcatga ttttcttgat atctaaatta tcaaagccga atttatgggc
      601 agcttttagt aaattctttg tatgaaggat tccgactact ttatctttgt cttcgttata
      661 aacagggatt cttgaatagt tttcggataa aacttcattc acattttctt ccacagtgtc
      721 gttgatatca atcataaatg catccgttcg tgggaccatg acttctcggg caactttcgt
      781 atcaagagaa aaaacccctt ggagcatttc taattcttca ttattcaata cgccttcgtt
      841 ttcaagcata taacgcattt catctctggt catttttgaa tcttcatcat caaaagtcat
      901 cggcgtgata cgcgccaata aacttgtgga agcagaaagc agccagacga agggcttagc
      961 tactacacca atcaagcgta tcagccctga agtgaacttt gcgacttctt ctgatttatt
     1021 taaagcaata cgtttagggt acaattctcc aaaaacaatc gatacatagg tcaaaatagc
     1081 cagcacaatg atatttgcaa tattttttgc cgctgtgccg ccacctaata ccggagctaa
     1141 ccgttccgat aaagtattag ctagtgaagc accagacaaa atgttgacca aagtgattcc
     1201 tacttggatt gtcgatagga aattatttgg attctgcaat atttttaaaa gcttttgaga
     1261 tttcacatct ccttcatcag ctttttgctc gattcgattc ttattgatcg aaacaacagc
     1321 aatttcggaa gctgctaaaa aagcattgat caatgtcaaa acgatcaata ataaaatctg
     1381 cgctaacagc gactgactct cagggtcagc attcataatc gcattctctc cttataaaat
     1441 taatttttag tttacgatag aaaaaaacgt ggcactatag ccacgttaaa atggtatcac
     1501 aaagcaattt tctttgcaat aaaaacaagt gtactaaaac tacctacgag gtcgtagcaa
     1561 ttttcttctt gttttcattt tgcttgtctt ctttgacgat tttgattata taggaaatga
     1621 tcgtcttgca gattgcgtaa aaaggaacac ctaagaagat accaagcaat ccggcaatat
     1681 ttccagctac tagaagaacg ataatgatcg ttaatggatg gattttcagt gatttcccaa
     1741 ttacattagg ataaatgata ttcccatcca actgttggac gattagcaca accacacaac
     1801 aaagtaatgc ttttaccgga tcattgaaga ctgttactag aaatgcaggt gctagtccta
     1861 gataaggtcc taaatatgga attaggtttg tcaatcccgc aatgacccca aataagaaag
     1921 cataatccac accaattacc atatatccta aaaatgtgaa ggtaccaacg aataaacatt
     1981 cgattgcttg tccgctaatg taattagcca atgttttatt cagttgcccc aataactcca
     2041 caatttggtt tcttcttttt tctgggaaaa actgttggat gtttggcact agtttttctc
     2101 cgtctttcaa catatagaac aagatgaatg gtgctgtaat gattactacg gttgccgaag
     2161 cgatcgttcc aacgatcgaa cctaaactat tggatactcc actaaggaat tgctgaatga
     2221 ttgtcccgta agaaatgtct aattgctcaa aatatttatt gatatctact tccttgaaca
     2281 aaggcaattc ggctacctct tttaaccagc tttctacctg tttgatgaaa tcaggcatat
     2341 tagatgccaa tgaggaaatt tgctgtacta gacttgggat gacactaagt aaaatccaaa
     2401 taattgctgc aactaaaagg agcagcacaa ttgcgacagc ccatattctt ttgatttttg
     2461 ctttcatcaa cagcttgaca ataggattca ataaatagta aagaaagcct gcaatcagta
     2521 caggtgcaaa taacgtggaa aaaaatgtcc cgatcggttc gaaaataaag tttatttctg
     2581 atgaaatcat gatcaatgca gctatgatca ataattcaag cgaccaaaac attagtttag
     2641 actgtctaag tttttcaaac aatagatttc ctcctttctt tcttactagt atatattata
     2701 ttcgttaaaa attcttttat tcttttttta tttttgaaaa cttgccatac tttttatttg
     2761 ttttattttc cagtatacta tacgaaagga aggtggctag accatgaaag ttgtacaaac
     2821 aaaagataca atgagcaata tttacttaga tgctgtacgg atcaggcatc aagtttttgt
     2881 agtcgaacag ggtgtgcctt tgtctcgaga aatagacaaa gatgaagccc attgtatcca
     2941 cttcgtcctg tactcagata aaaaagaacc gcaaggaact gtacgtcttt tgcctttaga
     3001 aaacggaaaa atgaaattac agcggatggc gattctttct gaatacagac accaagggtt
     3061 aggaaaaata ttgattgaag aagcagaaaa ctttgctaag aatcaaggct ataatacgat
     3121 tttattaggg gcacagtcca cagctgaaac tttttacgaa aaactaggat atacagctta
     3181 cggcgatcct tttgaagatg ctggcatgcc tcatatttat atgaaaaaga ctttataaaa
     3241 atttatttcg aaaaaagcaa aaaacagcaa gcttttgaga aaaatctctc actggcttac
     3301 tgttcttttt tgtgtttact tacgcgacaa ttgcttgtaa cggttatacc aaatatcaat
     3361 atagccttcc gagaacgggc catctccatt attgatccag tcaaccagaa tcttcacatt
     3421 ttcttttagg atgaagtcaa tatcgttcgg gtaattcatc attttgctgt gcatctcata
     3481 ttcatcgaca tccaatagtc gtttctcgcc gtcaggaaat accttgatat ccaagtcata
     3541 gtcgatatat ttcaaggcct catcgtccag caaaaaaggt gaagctagat tacaataata
     3601 ggaaaccccc ttttctctga tcattgctat tacattgaac cagtattttt tatgaaaata
     3661 aacaatcgct ggttcgcgcg ttacccatcg gcgtccatca gattcagtga ctaatgtgtg
     3721 atcattcacg ccgatcatag agtgttcgct tgtttttaat accatggtat ctcgccacgt
     3781 tcgatgcaat ttgccgtcgt gcttgtaact ttggatcgtc acaaactctc cttcttttgg
     3841 aactcccata gcaacccttc cttaagcagc attttgtgct ttgtgtttgg gtgttcatta
     3901 caccgacatc ataattatta tatcaataaa aatgagcttt gcctagaaag gattcctatt
     3961 gtttcgattt ttctctaact gatctaccag tttcatttgg accttaggaa aaacgaccga
     4021 atcaaatgca tcaggcttca accacttcga agttttgttc tcggtaaatt cttgttctgc
     4081 tccctcggtt gcccgtccgt aaaaaaggag aacatgccat ttcagatgac taaaaatatg
     4141 agtaacttct cctaaatgtc gtgtctgcca aacaaagagt tcctgctttt caaatgggag
     4201 attttgctct tcttctgcta ctaggtcatc aaacagatcg atttctggct ttgtttccca
     4261 ttcttttttc aatcgctcat actcctcttg tgtcacttcc atcattggaa atgtccacat
     4321 attcgccagc aatttttggc tatctctttc ttcaaaataa tacgctccag aatgattttg
     4381 aagagctgca ctgatataat aaacgtcttt tggctttgct ttttttgttt ttacaggaaa
     4441 actcgtttgg attcctcgtt tgttagctaa acaaaaggct tggatcggac aagtctcaca
     4501 ctttggagaa gttggtgtac agatagctga acctagatcc atcattgctt ggttgaattc
     4561 acctggatgc ttttcatcaa tgattttccg catcgcttca tcaaagattt tcctgctaga
     4621 agcttttgcg atgtctgctt caatgcaaaa caatctgctg actactcgca tcacgttgcc
     4681 gtcaactgca ggttctggaa gtccaaatgc gatacttgca atcgcgcctg tcgtatatgg
     4741 tcctattccc ttcaatgagc taatttcttc aggcgtttgc ggcatttccc catcaaattc
     4801 tgacatgatc tgtttagcag cagcttggat gttccgggct cttgagtaat aaccaaggcc
     4861 ctcccatgct tttaaaagct tttcttctgg agcatttgct agctcttcaa ttgtaggaaa
     4921 ccattccatg aaacgataaa aataatcgat cactgtgtct actctggttt gctgaagcat
     4981 gatctcagag atccagatac gataaggatc acgattatag cgccaaggca ggtttctttt
     5041 ttcttgttca taccattgaa tgaactgatc ttgaaattct tttgtttctt tatctgtcca
     5101 tttttcccat tctttattca ctttcctctt ccttttccgt aatgaatcgc tggatcatat
     5161 ccaaatcaaa tcctttttga tataagcttt gctttacttt tagatttctt tttttaggtt
     5221 caaaccttaa atttcttcgc catagtttat ctccttgttt gactaaataa tcatattcta
     5281 tttcttgatc tgcttcttgc ggaagatcag ctatcgcttg ttggatcact tcttgagaaa
     5341 agcctttcgt catcaatgtt tgttggattt ttctcaataa ttctttttgg ctttttccgt
     5401 gattttttcc aaacgctttt tcggctgtct gatgtgcatt ttgaacttgg tcttcaaatg
     5461 tatattggct gagcgcttgt tcaatgattt caggtttcaa acctttttgc tgcatctgct
     5521 gcgctaattt ctttggtcct ttgtcactta agcgcatatt cgtgcgcaca taactttccc
     5581 catagaccaa atcatcgaca aggtccaatt cttttaagcg ggctacgatt ttatttcgat
     5641 cttcttgaag gatttccttt tcttttaagt atgttcggat ttctttttcc gatcgcaact
     5701 gataacttaa gtaattcatt gctaggtgaa aaccgaaatc agctccgcta ctcttcttga
     5761 tctcttggat agtttcttcg tctaattccc gtccttttaa taatcgatag cgtactaata
     5821 catcttcaga tacttgtaaa gcttctcctt cagataaatc tacgcgatag aaagggcctt
     5881 tgcctttact gattctcgta acggttaaca taaacatcct cctcgaacat atattctcta
     5941 tcataccaca gaaaatcccc gtaatcaggt attatatgtt agaataaacc acagaaaaaa
     6001 gaaaaaggga gatacttatg gcagaaacaa ttgtcaaaac tggacaaacg gttcctttga
     6061 aaatcaagcg tttaggcata aatggtgaag gaatcggtta tttcaaacgg ttgatcatat
     6121 ttgttccata tgcactacca aaagaagaag tactagtgaa aatcacgaaa gcaactcctc
     6181 gctatgcaga aggagaatta ataaaagtca aaaaaacaag caaagatcgc gtagtggcac
     6241 catgtcctgt ttactatgaa tgcggtggct gtcaattaca acacttagct tatcaagctc
     6301 aattggattt taaaaaggat ctgcttttgc aagcattgga aaaattcaaa ccagcaggct
     6361 ttcgatctta tcaacttttg ccgacgatcg ggatggatga gccttggcat tatagaaaca
     6421 aagcacaatt tcagttacga aaaaataagc agactcaaaa aattgaagct ggtttatacg
     6481 aagcaaactc tcatcagtta gtccctataa cagactgctt ggttcaagaa cctcgaacac
     6541 aaaaagtaat gaatactgtt gttcaactat tgaataaatt tgatgcaccc atctatgatg
     6601 aacggacaaa cagtggaatc ttccgcacaa tcatggttag aatcggtgtt aaaactgggg
     6661 agttacaagt tgtttttatt actcgcagta aaaaattccc gcaaaaaaac gcaatgatcc
     6721 gtgagatcaa tgaacgactg cccgaagttg tttcaatcat gcaaaatgtc caggcacaaa
     6781 aaacgtctct cgtgatgggg gatgaaacga tccacttatg gggaaaagaa gccattgaag
     6841 aacaaatcaa tgaagtgact ttcgatcttt ctcctcgtgc cttctttcaa ctcaatcctc
     6901 agcagaccga attgctttat ggcgaaggaa tcaaagcatt agatgtccag cctaatgaaa
     6961 cgatcgtgga tgcttattgc ggagttggga cgatcggctt aagtgtagca aaacaagcca
     7021 aagaagtacg gggaatggat accgttcctc aagcaatcgc tgatgcaaaa aaaaatgcgg
     7081 aaagattagg atatcacaac actcgatatg aagtcgggac agcagagcaa gttctgccta
     7141 aatggttgaa tgaaggtttt cagcctgatg gtatcatcgt agatccacca cgtacgggtt
     7201 tggataacgc attgatccaa tcattattga aataccctcc aaaaaaactt gtttatatct
     7261 cctgtaatgt ttctacactt gccagagatc tggttaattt aagtaaaaag ttcaagattg
     7321 aatatttaca gtccgtcgac atgtttcctc agactgcacg ttgtgaagtt gtcgtgaaga
     7381 tgacgagact cgctgactaa atgaaagagg ttgtgaaaaa gtctgccctg tatcaagtaa
     7441 taagaacagc caaacaaaaa tagctcttca tgttttatga atactgtact tgtgaccgaa
     7501 gagttaacag gaggacacta tttatttaaa aaaagggttg tgacatagtt tttgtcacag
     7561 ccctttttga ttattattgg atattcacgt ctatcacttg ataaaatgca tttgtcgtat
     7621 cgctgatatt ccatactgct aaaatcacat tatatccttt tcgatcctgc ggaagtgtga
     7681 tcgtttggtt gactgttttt ggtggaacag ttgctttgtc gtctattttt gtgatcaatt
     7741 caaaatcact aaatttcaaa ggttgattag gattccaagt tggtttggta atgtagtaat
     7801 cccaagtact tgtccgatgt tgtgctgtta aattccaagt aatatttaat gttcctggat
     7861 taaccatgtt tttatgccat ctgttagatg tctgttcatc taaaggctca aagccgctga
     7921 ctcctgcact agcaattttt ccatcaatga atgtattttt ggtagcttcg atactttgcg
     7981 gttcccattg tgctcttccg acattttgat ttaagtttcc tgctgctgat gttcctaggt
     8041 atgcacggct tcctggagaa gtaatataac catgcgcaaa acctggaatg ctaaaaccga
     8101 acaaccctaa actaactaac ccaataaaca ataacgtact aatctttttc atgtagaaac
     8161 ctccttcatt tgataaccac attgtaaacg ctttattgta ttaacacaaa cattatgctt
     8221 ttataatttt atgttgaagt aaaaaaggtg aaaaaagtaa gatgcagatt ataacagaag
     8281 tagacgagaa aagtacaaat agaaaacatg agaatcaaat catatgatct ttttcaaaaa
     8341 catcattcat ataaacaaat gttcaacttt ttaataacaa caattatcaa tttttttctt
     8401 acttcacaaa ctaaatacag gtcttgctgt gagccttcat ggtaaactaa tattatagaa
     8461 aggagttagt aaaatgaaga tgataaaaga tattcgtttt tgggtttgtg ttataggaat
     8521 tgtcatatta ggctttttat cagggttgct tagcggaaat cccggaaaat attactattc
     8581 tttgcaactt ccaccatttg caccacctag ctggatattt gggccgatgt ggaccttact
     8641 gtatatattg atgggtattt ctctttattt gttacttaat cacaacaaca aaaaacagcg
     8701 aaacaatctt gtcggtcttt ttgtgattca gttcattttt aattttatat ggtctgctct
     8761 attttttaat ttaagaaata ttttcattgc agcaatcgat attacacttt tagttatttt
     8821 cttgtcagtg cttatgtatc agttgtggct gcatcatcgc ctggcaatgt ggctcatgat
     8881 tccttattat ttatgggtac tattcgcaac tctattgaat tattcaattt attttttaaa
     8941 ctaagcgcta aaaagagacg tcctgtaatt taacacatag aaaaagtgcc acctaagtta
     9001 gattttttgt ctaatttagg tggcactctt tattaaccaa actatttttt tctagtaccc
     9061 acttgttgtt ccatactcat tattctggtc ataaccataa ccattttgtt cctgtccagc
     9121 gtaaggatca taactttgag tatcttgact tggtgtatag tactgacttg tatcttctgt
     9181 ttgctgataa taggtattcg gatcttggta ctgatcatat gtgttttctg aataggaaga
     9241 ataatcggtg tacccttcac tattcgatcc atcaccgttt gtcgaatcgc catcttccga
     9301 atcatcaaag aacatcattg aagcattctc atttttcaaa ttaggtaatg tatcgcttgt
     9361 ttttatattt aattgtttct tcagttcatt ttggatcgaa agaagatcat tttttcctaa
     9421 aatttgatag tagatatcat tgatcatttg tccttcgcct tgcaactggt cttgtttgat
     9481 tgtttcaaac gccggggtgt agttcgtcgc tacatcaagc atgtcatccc aatctaagtc
     9541 cgtcgtcaca ttgttctcaa ctgcttttaa aatttttcta taattgctta cgccatctaa
     9601 acttaaaact ttattgacga ttttcgtcac tacttcccgc tgacgacgtt gtcgaccgat
     9661 atcgccttct ggatcttcgt gtcgcattcg agaataggct aaaccttttt ctccattcaa
     9721 tgtttgttca cctactggta catggacacc atccaaagta aagtcaattt tattattcac
     9781 tttgatgcca ccgaccgcat tgatcaaatc ttccatgcca tccaaattga tcgttacata
     9841 atgatcaata ggaatatcga gcagttgctc aattgaatcc atggccattt cgacaccacc
     9901 aaatgcgtag gcatgattta atttatctac cgttccataa cctacgatat tcgtataaat
     9961 atctcggtcc aaactgatga tcgttgattt tttttgtttc ggattaactg ttactaccat
    10021 catcgtatca gaacgtcctt gttccgttct tcctaatcca ccggtatcta aacctaatag
    10081 caaaaccgaa aaaggttctt tgtcatcaat gcttactctc tcagaacgtt tggaggatat
    10141 tcgattagag tttttagaaa ttttgtttac tgtttggctc gcttctccca taaatctgat
    10201 tccgtatgct gataatccgg caatcagcaa aacgaatacc cctaacaagg ttaatagaac
    10261 tttttgccat cgcttcataa tacacctctg tttcacattt tctaaaatat tgtctcatat
    10321 aactgttcaa tcagcaaatt cactttttaa tcataagaaa actttaaaaa acatttttgt
    10381 gtaacgatta ttatttttta aaatttctca aaattcttaa ctatcatacc ataccgtgaa
    10441 aaagatcgaa agaccgaata cacgcagttc gcaatcgttt tatctttttt tatgtatttt
    10501 ttccagaaaa agcgaacaac aaggtatttt ccaaataatc gtgcagtaaa atgatcgcac
    10561 atacctttcc catcttttta acagtctttg cctaccgttc agattatcat tcgatttcac
    10621 gacaatttct tcctatgact ggtccttttg ttaaacccat cttctattcg ttttatttcc
    10681 agctcactgc atggctctta aaatgactcg aactagtgat tgcattacca tcatctttta
    10741 atggatagat atgctcctct tcaaagtaaa acgattttct tttaaagtaa tagaattgac
    10801 atggtaatga gtatgtatac tagtggtttc acaaatggtc ttttattcaa catccaaaaa
    10861 ctgaaatttc tttaaaatcc attactaacg aagaaaatgt tccttgcttg ataattagtt
    10921 aggacatgtc aagtgaacaa atgcatttac ttagccaaaa
//
LOCUS       DAFEOM010000083        10661 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.086, whole genome shotgun sequence.
ACCESSION   DAFEOM010000083
VERSION     DAFEOM010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.086"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            333..2279
                     /locus_tag="LP524_002395"
     CDS             333..2279
                     /locus_tag="LP524_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL2235414.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
     gene            2282..2737
                     /locus_tag="LP524_002396"
     CDS             2282..2737
                     /locus_tag="LP524_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL2235415.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            2751..4079
                     /locus_tag="LP524_002397"
     CDS             2751..4079
                     /locus_tag="LP524_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBL2235416.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            4113..4397
                     /locus_tag="LP524_002398"
     CDS             4113..4397
                     /locus_tag="LP524_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2235417.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            4399..4914
                     /locus_tag="LP524_002399"
     CDS             4399..4914
                     /locus_tag="LP524_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HBL2235418.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            4930..5592
                     /gene="rpe"
                     /locus_tag="LP524_002400"
     CDS             4930..5592
                     /gene="rpe"
                     /locus_tag="LP524_002400"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBL2235419.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            5599..6171
                     /locus_tag="LP524_002401"
     CDS             5599..6171
                     /locus_tag="LP524_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL2235420.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(6358..7878)
                     /locus_tag="LP524_002402"
     CDS             complement(6358..7878)
                     /locus_tag="LP524_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBL2235421.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGIQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(8121..8306)
                     /locus_tag="LP524_002403"
     CDS             complement(8121..8306)
                     /locus_tag="LP524_002403"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HBL2235422.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(8290..8472)
                     /locus_tag="LP524_002404"
     CDS             complement(8290..8472)
                     /locus_tag="LP524_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235423.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            8713..8931
                     /locus_tag="LP524_002405"
                     /pseudo
     CDS             8713..8931
                     /locus_tag="LP524_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            9426..10025
                     /locus_tag="LP524_002406"
     CDS             9426..10025
                     /locus_tag="LP524_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL2235424.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            10089..>10661
                     /locus_tag="LP524_002407"
     CDS             10089..>10661
                     /locus_tag="LP524_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage abortive infection AbiH family
                     protein"
                     /protein_id="HBL2235425.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIGD"
BASE COUNT         3525 a         1675 c         1988 g         3473 t
ORIGIN      
        1 taggactcaa ttgttcatag atgagcattt gtaattaagt tatattataa taaaaaaata
       61 cgaattttag tgacgccatt atattgtttt tatttttcaa ttgtaatatt tcatattact
      121 tccataataa ggagtatttt ctattggaat atcttagctc ttttaaggat ttctcctttt
      181 ttgtttctca ttttttaaga ttatttggat ttcctttaca acaaacttcc acaagtatta
      241 ttgacataga tgtaattgag ggaagggggt tccagtgcta gaataaataa tgaaaagagg
      301 taacaaatgc tagcgttaag aggtgaacaa gtttgattga aagacatgta aatttattaa
      361 aattattaag tcgacaagaa gactttaaac ccgcttcttt ttttagtgat aaattggcag
      421 tgtcgaccaa gaccatttat caagatattg aattgttgga agattatgta gttaattacc
      481 aagtaacaat tgagcgatta ccttctttgg gtatcaaact cataggtgat aggaaaaacg
      541 ttgaacattt gatttcatcg gtagaaaatt atcttgaaaa agatatttat tctccagaaa
      601 aaagacgtgt cgagatcatt aaagaagttt tgtttggtga taagcctgtt tctttaactc
      661 atttgtctca aagttttatg gttagtaaaa catcgttata caatgacctg agaattatca
      721 ataaaattat tggcagtgga gaagttaagt tgcagtcatc tgcagaagga attagtttta
      781 ctggtaatga acaagcaatt caacgagcaa tcaagcaact tgtattctat tatgcgaatg
      841 acatggaatc aaaaacattt tttcaagttt taaatatgtt gtttgattca aaagttatta
      901 gtgtcattca tgatttgcta ttcaatgatt acagtgaact gactgaaaag gtttctgatt
      961 actatgttcg gtcattgatt tctactttag ttatccaatg taaccgttta ctgaaaggtc
     1021 atcatttaac tgttgaagaa gaattcctct tcaatagcat tcgctatatg gaaacgtaca
     1081 ttgtggcaaa tggtttaatt gaagaattga aggcaaaatt gaatcttcca ttttatgacg
     1141 cagataaaga atatctatgt cgtcaactat ttgcacatcg tgtgacaaat aatttaaaaa
     1201 ccagcaatga agagtacgct atgagcgttc ataaattgat tgaaagaatg agtgagattg
     1261 aaaaaattga tttgactcaa aatgatcgat tatatcaatc cttattgtat catgtgccag
     1321 caatggtcct tcgtttaaaa aaaggtatta aaattcaaaa tccgttatta gaaaatatca
     1381 aatctcaata ttcagaactc tttagcatag tatggtacgc catgtccatc cttgaagaag
     1441 aatttgatgt gactttaacg gacgatgaag tttccttaat tttgattcat tttcagattg
     1501 ctattgaaga tcagtccaaa gcaaataata tcatcattat ttgccaatac ggtatttctt
     1561 ctgcgcaatt aatttacaat aaagttcgac ggtttttacc agcaaaagat aatgtagaaa
     1621 tatcaacttt agataagttt catttagctg aaattgggga agttgatttg attatttcct
     1681 ctctttcctt tgatgcaggt ggtataccct tagtaagggt atctccattg gtaagtaaaa
     1741 cagattatat gaatattatg gaagcttata ctaaatattt aatagaagaa ccttcgcgat
     1801 ttattcaaaa tgaactgatt cctaaagcgg ttaaagttcc caccatatct aaatttgttc
     1861 aatcatcttt agtaaaaaca ggagtacagg tggaaagcaa agaagaatgt ttggatttga
     1921 tgatttccga actagaagcc aattcattag ttactgaaga gtttcgtgct tctatcttct
     1981 ctagagagaa aatggggaat actaatttag aaagtggtgt tgctttaccg catgcgaatc
     2041 catcaatggt cattgaatcc aacattttta ttgtagcatt aaaaaatgcg attaattggg
     2101 gcgatcgaaa agtcaaatta gtcattatgg ttagtttagc tgaagaagat gtatctgaga
     2161 ttagaggagt atatgaagaa ctttaccaat ttattgggca aaaagaaatg gtcgatcgct
     2221 tgttacaagt tcatgatagc gaaaaattac taagaatttt tcaacaaaaa ggagaataaa
     2281 aatgtatcta gataaagaat tatgtttatt tcaattagaa gtgaaagatc aaaaagaatt
     2341 gtttcaagta atgtctgaac aattaaaaaa cgctggttgc gtgaaagata gtttcttaga
     2401 aggtatcacc aaccgtgaac aagaatttcc aactggatta gaagtaaatc aaattgggtt
     2461 tgctatccca catacagatt ccgcacatgt aaatagctct caaatctgtt ttgcctcatt
     2521 aaaggaacca ctggtatttt cagatatgac agataaatct catgaaatac ctgtacgttt
     2581 agtctttatg ttggcaatgt cacaacctca tgaacaaatt gatacactac aaaacttagt
     2641 tagcctattt caaaatgaag aaaaagtaaa tgaattactt gcatgtacca cgaaagaagc
     2701 atttattgag ttagtacatt ctgcaggagt ttattaggag gaatagagag atggatattg
     2761 tattaaatgc ctttgaatgg tttattggat tagggtcaaa cgtcttttta ccactgatta
     2821 tttttgtaat cgggatatta tttggattaa aacctggtaa agcatttgtt tccggcgtta
     2881 ctgttggtat cggttctatt ggtttaggat tagttttaag tttattatca gatggtttag
     2941 gaacagcaat tcaacaaatg ggggaacaat acggtggtgg cctgaatatc ctagatattg
     3001 gcgtaggtgt tggtggacct ttggcttact ctacatcatt gggaatttta atgattccaa
     3061 tttcattaat tcttaacttc atatttgtgg ttttgggttg gacaaaaaca ttgaatgttg
     3121 atatttggaa cttttggttc gcaattttcc taggtatgat tactcaatct gttacgggaa
     3181 acttttggtt tggcttaatt ggcgcgattg tcgcagtaat tttacaatgg ttcttagcag
     3241 atcgtacaca aaaaactgtt agcgaatttt tcggctatcc aggaatctct atcacacata
     3301 tgatgggatt atcaggagta ctatttgcca aaccagtaaa ttgggttttt gatcgtattc
     3361 ctggatttaa taaaatagat gcagatgcag aaacattaac aaaacgtttc ggtgttttag
     3421 gtgacacagt tgttattggc ctaattatcg gtattgtagt gggtattatg gcaggctatg
     3481 acgtagcagg aattggaaac ttaggaatta tgactgcggc agtgatgaag attatgccta
     3541 aaatggtagc aatgtttatg gaaggtttaa tgccaattgc agaagcggcg aaagaattta
     3601 cgaataaaaa gttaaatggt cgccaagtaa acattggaat ggatgcagcg ttaactgtgg
     3661 gtcatccaac tattatgtca acttctctat tgcttgtacc aatctcatta ttgctagcac
     3721 taattcttcc aggaaataaa gtattacctt ttggagattt agcattcttt gctttcgcca
     3781 tctgtttaat gattccttat tttaaaggaa atattgtgag atcattagta ggatgttcgt
     3841 tgtatgttgt tacaacatta tatatggcaa catggttggc accaattatc actgatgtgt
     3901 ttacattagc aagtttcgat gtggggacag caggattagt aactatgttg ttatgtggat
     3961 tatggccagt agggttgttt gttttattag catcttactt tggtgtaatt ggaataggtg
     4021 gattaggagt tgttttagta ggtgcttcta tctacatgaa caaagtgaag aaagcttaat
     4081 cataaaatta aaataagaaa gaagagatca atatgaaaaa attattagtt atttgtggag
     4141 caggtcatgc tacttctaca attgcagtag caaaattatc aaaatggttg gaagaagaag
     4201 gattaacaga tcaagtgaaa atttatcaat ctaaaattgc tgatgagcta aataaaattg
     4261 atgaatatga tgctgtagta agtacaacaa ttgtccctga tgtaattaaa gctaaagtaa
     4321 cgaatggctt gggcttatta acaggtattg gtacagatgc catttatgca gatgtaaaag
     4381 aaagattagg tgtttaatat gcgttttcat catgacactt tgactattga aacagtttca
     4441 gggcgtcctt cgtatcatga tattcgaaaa gaacttttag atacagttga tacagcaggt
     4501 attgagaatg gaatgttagt aatttcgagc ccgcatacaa cttgctcact cttttttgaa
     4561 gaaacgatgc atgacacaaa cttttttggt gatgattatt tacatgtaga catcaacaat
     4621 gtaatggaaa cgattattcc aaaaatgacg agtgaaaatc aatacaacag tccggggcca
     4681 aaacacattg attttggaac aagattatct gatcccaatt accctgcaga aaaatgggtc
     4741 atgctaaata ccgatgcaca tttacgttca tctatttatg gaaccaactc aatgacttta
     4801 attgttcaag aaggtaaact tttattagga gaaatgggcc gtgtctattt tgtcgattgg
     4861 gatcaactaa gagagcgaac tagaacaatc aatctcatga ttatgggaga ataaacgggg
     4921 gaatagagaa tgacaattgc tccatcattg ttaaatgcac ctatcgcaga agttaaagag
     4981 ttatttgaaa tcttaaaaga aaagaatgtt gattatttgc atattgatgt aatggatggc
     5041 tgttttgtac cagatatggc ttttggacca agtgttatta aagatattcg tccactaaca
     5101 gacgttgtgt ttgatgttca tttgatgatt gatacaccag aaaaaattgt cacaagattt
     5161 gtagaagcag gagcagatat tgttaccttc cacattgaag ctaccaaaca cgcaatgaag
     5221 gtcattcagt tagtcaagga agcaggagca aaagtaggga ttacttttaa tcctgcaaca
     5281 ccagtcagtt ttattgaacc agtattatct ttagtcgatc aagtattagt aatgacaatc
     5341 aatccaggaa cagagaataa gcattttatt caagagacag tagttaaaat cgaacagtta
     5401 gatgtaatac gcaaacaaaa tgattatacg tatgatattg aagtagatgg caagattgat
     5461 aatcaaacga ttaaagtttg ttctaaagca ggggcggata tttttgtatc aggcggtttc
     5521 atcttcaatg ggaaaaatac cccgcaagaa caaattgata aactaaatca atcattagag
     5581 gaagtgaagt aaaagatcat gaccaataaa gaccaaaata aaaactattt agaagcgatt
     5641 atcttagaat tacaaaataa ctcaaaacgt attaattctg tagagttaga acaatttagt
     5701 gagaaaattg ttcaagctaa acacatcttt ctagctggtg caggacgctc tggaattgcc
     5761 atgcaagcat ttacgaatcg cttaatgcat ttaggttttt cagtcagcct tgtcggtgaa
     5821 attagttcac cacattcgca acctggagat ttattaatta tctgttctgg ctcaggtgaa
     5881 acaggtagtt taaaaaattt agcggccaaa gcaaatcaaa gtggtgtaag cattgcttta
     5941 gttaccatga aaaaagactc gtcgattggt caattagcag attctgttct tgtattacca
     6001 ggtattacca aagatgaaaa tcaacgagaa gaagatactt tttcacaacc tatgggttca
     6061 agttttgaac aattagcatt tctcaccttt gatagtttag tgttatcttt aatggataaa
     6121 acaggtgaaa gctcagaaac gatgtttgct cgacatgcag attttgagta gtagatttta
     6181 aagataaaaa accattctct aaatgctaat tattcagcaa atatgagaat ggttttttta
     6241 tttgattaga cataacttaa taattcagta gttttgagcg taagcgtgtc atagaatggt
     6301 acctaaacac aaatagcccg atttgataca ctcaaatcag gttatttgtg gcttatatta
     6361 tttacagatt tcttgtatct gctgattcca tggcaaataa gcgcgtagcg cttcaggtgt
     6421 catcgaagac agattgggaa tctctgtaaa aagatgattt aaatatttgc ggatgtttaa
     6481 cccattcgca acagctgtct ggataatact caatataatt ccatttgatc tagcgccttt
     6541 taagctttta gaaaatagcc agttttttcg accgatcact aactctttga tggcacgctc
     6601 tgctcggtta tttgaaagtt ccaagcgtcc atcaagcaac acaatctgaa ttccgtcttt
     6661 atgattgact gcgtattgaa ccgcctttcc aagctgcgat tttggcaaat aagcaaaact
     6721 ctcacaccaa tgaaaaaatt cagtcatcac aggctgcaac gtttccttcc tcgctacaaa
     6781 cctttcttct gcgctaagct tattaaaggt ccgttcgatt cgaaacatct tgtcacagaa
     6841 gtctaatcct attttggcat gactttttgg ggacggttgt ttacccaatg cttcaaaaaa
     6901 ctttctgcga acatgcgccc aacaataaac attgtttacc ggcagctttc cataggccgc
     6961 gtaaccatca gtatgcaaga aaccgctaaa tgattctaaa aaactttttg gctcatcaac
     7021 ggaacgcgaa tttgcgtgct cgtagagcac aacttgtttt tcttcgcttt tacctgaagc
     7081 atataaccag taaaaggtct tttctttatg agtattcaat acccgatagc ttgtctcatc
     7141 cgcgtgtaag acctcctgat tgcttaattc ttgatgaagg caattgtaga ttggctctaa
     7201 aaccatttca cttaccttga tatgccaatt agtgattttt ttacgatcaa gagggaaacc
     7261 aagtgcatgc cattcttttt cttgtcgata tgccggcacc tttttaacaa atttttccca
     7321 aatactctgt gtcacaattg aagaggaacc tagactatta ttgatcaacg gtttaggaac
     7381 aggcgctttt ttgatttttg tttctcctaa tgcttcacat tctctgcaat gataggcatg
     7441 ttggtagatg acttgttttt ctatatgggc aggaacatac aatggctctt ctctaagcct
     7501 tgtcgcaccg atatcatgta aagtatgacc acagtcttca cacaaacaat cggcaccttt
     7561 taattcataa tgaataggaa cttcaggcag accttctaat gaaagtgctt tgagtccctt
     7621 gcgttttctg cgtttaaaag aagataccgt aatgggttcc tggctttctt gtccagtgtg
     7681 ctctggttca ttaaaagttg agtcgtctaa taaatcgatt tgcccatcta cgggtggtgt
     7741 ttcttttttt cgtccaaaca agcgtcgatt catttcagca atttgttctc taagaagagt
     7801 attttcctgt tgctggattt caagcaactg tgttaattgc ttgatttgta caagaagttc
     7861 agattccatt tttgtcattt ttacaccacc attctagcga ttttcttaca tctattatac
     7921 cattttatga caacgaaaaa cagacaaaat ccttgattta acaaggtttt cgtctaatat
     7981 atcgcacctt ttttcgtttc ttttagcgta catttttctt cgattgaaag gccagtcagt
     8041 aagacttctt ttctggaacg cggccattga aggtacccac tttcaaatct tttgtacatt
     8101 aatacaaatc cattttgatc ctagtaaaga gccttgaagc gatctttctt cgtaccgcaa
     8161 aataagaaaa gcgcttgatc gtaaacatca aggtcatatt cttctataac gaaagctgct
     8221 agtccatcaa ttccttttcg aagatctgtt tttccacaaa cgataaaaat atgtttcaat
     8281 gcagttgcat caatgagcat tggcattcaa ctcttttaac aaggtataca aaagatattt
     8341 atccattcca ttataaataa atatttcctt atcttccgat ttgattctgc aagctagttt
     8401 tggcttggtg tcaatttgcg atttttgagc cgcacttgaa aattgaactg gtacaatatc
     8461 ttttttattc attcgttttt ctccttctat atttgatagt ttaattatag aagaaagata
     8521 atcggcgtac gagatactat cctatgacac gcttacattt aatttaatat tttgcctcaa
     8581 attagccaca ctttttatag aactaagagg aaaaatgaag ttttcttcct attaatatag
     8641 ttgtttgaaa tcttaaatat agcgttttac gatgtaatgg gaagtatggt gaataaatca
     8701 actatgttcg tacgaagcat ttactcaact aatttaatcg aatctttcaa taaacaaatc
     8761 aagaaataca gccaccgcaa ggaacagttc caaaatgaag agtcgatgga acgtttctta
     8821 gtctcgtctt ttgatactta caaccaaaaa ttcctaggtc gcagtcataa aggtttccaa
     8881 caggccgaag gcgagctcga acaaatgctg agtcaaccga tggagaatta gaaacaatct
     8941 acagtaggga agaaccattg acacaaaatt attgacgctc ccaactaaaa gtgtcaaaat
     9001 aatgatgaaa tcatttagtt cttataaatt agaaaagaga aaataaatat tccatatcta
     9061 ctttattgga tccgtaacat gactaaattc tgaatacttt ttaaagctta tcatttggta
     9121 aaactgaata taagttaatt atatagactg aaaattttga attttcagaa tagaataact
     9181 taaaagtact gacaaaattt tttaaagggg caatattaat gttctgagat aatcaaatcc
     9241 taccgaaaat atcaaaaatc caatgtgaca caatcagatt gtgtctcatt cgagggaatc
     9301 acacaatccc acgtggctct aggcagaatt gaatttttgc ttggggcttt ttccttatat
     9361 tgattttgaa tcagacttat tatatgttat aatgcaatta agtatcattg gagagggcga
     9421 agaaaatgaa gacggtagaa cctattcgag acaaaaaaaa gatcgatgcg atgaaggcaa
     9481 tcttggcttc gggaaaatat ggccaacgaa atttggttct tttttcgatt gggatcaaca
     9541 ccgcctatcg catttctgat ttgcgtcaat tgaagctggc cgacgtgctg gaaatttcac
     9601 gcggtcgcgt ggtggtcaaa gatcgattag cgatgaagga acaaaaaaca gctaaacata
     9661 attcggtcta tatttccaac aaattgcgaa aaacgatttt ggagtacgtt caacaggaat
     9721 ttcctgatca actccttcag caagattttg acggttattt gttccctagt cgaaaaggga
     9781 cgaatgaacc gctcaaccgc caaagtctgt ggcgaatcat tcatgatgct ggcacggcaa
     9841 tcggattgaa agacattggc ccacattcca tgcgaaaaac gttcggttac tttctctata
     9901 aacaggggac caaaacagaa atcattcagt ccttgttgaa ccattcttct cagcgcgaaa
     9961 cattgcggta tattggtatt acacaagaag acaaagatac agctgtaaaa agcttagatt
    10021 tataatataa aagttaagtt taaacatgga tagttttaaa ttagtagatt gagtgttggg
    10081 aggaaagaat gagtacatta tatattatag gaaacggttt tgatttaagt catggtttaa
    10141 aaactagata cactgatttt tcaaaatggg ttgaaaaaag tcatagtgaa atattcgata
    10201 aaatagacac aatgataaat gaaaataatg aggtgagaag tacagctgat gataagatag
    10261 gttggtttaa ttttgaaagt agtttgcatg agttaattca aagtccagtc tttgaagatt
    10321 atgtagaaga gtctaaggat tatctcatag gttatggaca tgaagattgg tcagattctg
    10381 cccatcatga ttttcaaaat atggtttatg attatgttgt ttctctagaa gaattacaac
    10441 aacttttcaa ggaatggata cgcaaagaaa tacagccgac aatagaacat aaaattccaa
    10501 aataccaatt ttcgtccgat gtactattct ttacattcaa ttatacagat gttttggaaa
    10561 ttctatacaa gcaaccgaaa gaaaacatat atcatattca tggcagttat gacgatataa
    10621 taatcggcca ctccgagaat tttgacaact ttataggaga t
//
LOCUS       DAFEOM010000084        10360 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.087, whole genome shotgun sequence.
ACCESSION   DAFEOM010000084
VERSION     DAFEOM010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.087"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            172..321
                     /locus_tag="LP524_002408"
     CDS             172..321
                     /locus_tag="LP524_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HBL2235426.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            332..1846
                     /gene="dltA"
                     /locus_tag="LP524_002409"
     CDS             332..1846
                     /gene="dltA"
                     /locus_tag="LP524_002409"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HBL2235427.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1843..3048
                     /gene="dltB"
                     /locus_tag="LP524_002410"
     CDS             1843..3048
                     /gene="dltB"
                     /locus_tag="LP524_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBL2235428.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3093..3326
                     /gene="dltC"
                     /locus_tag="LP524_002411"
     CDS             3093..3326
                     /gene="dltC"
                     /locus_tag="LP524_002411"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBL2235429.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3329..4597
                     /gene="dltD"
                     /locus_tag="LP524_002412"
     CDS             3329..4597
                     /gene="dltD"
                     /locus_tag="LP524_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBL2235430.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4738..5820
                     /locus_tag="LP524_002413"
     CDS             4738..5820
                     /locus_tag="LP524_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBL2235431.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5820..6623
                     /locus_tag="LP524_002414"
     CDS             5820..6623
                     /locus_tag="LP524_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBL2235432.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6693..7433
                     /locus_tag="LP524_002415"
     CDS             6693..7433
                     /locus_tag="LP524_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBL2235433.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7444..8448
                     /locus_tag="LP524_002416"
     CDS             7444..8448
                     /locus_tag="LP524_002416"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBL2235434.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8493..10337)
                     /locus_tag="LP524_002417"
     CDS             complement(8493..10337)
                     /locus_tag="LP524_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235435.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3338 a         1708 c         2090 g         3224 t
ORIGIN      
        1 tttaaaagta cgagaaatta taaagaaaaa agcagtgatt tgatgtgcta tttttataaa
       61 agctatgtga caaaactgtt agtaaaaaaa cggtttctgt aagctagaca catgtttctt
      121 tttgtcaaaa ttagttacac tttgtgtgag gttatatgag gagggaatcc aatgaacaaa
      181 tggcgttcta atccaaaatt gaattattgg ggaaaattca tcattcgaac aattttttat
      241 agtgcagtac tgctgttcct gatttatctg tatcactata aaaatattca aggaggaact
      301 tttatctata acgaatttta ggaggaagag tatggaaatc aaaacgatta ttgaagcaat
      361 cgatgattgg ggaataaatg aacctgatcg agttgcttat caggcggatg agacgcatac
      421 ttttggtgaa ttgaaacaag catcagacgc acttgcttat tatttagaaa aaaaggtaga
      481 aggcgatgga ccaattgtcg tttttggcaa tttggagttt gaaatgattg tctcatttct
      541 aggggttgtc aaatctggcc atgcctattt gccgattgaa gagcatacac ccaaggagcg
      601 tatcctatcg atctttcggg tggctaaacc atctatggtc atcagtatcg gggactggat
      661 agaagatatt ccttcggttc ctgtcataac aaaaaaagaa tttcaagaaa tcaagaagat
      721 accagtagga ttttatccca gaaatagcgt aaaaggtaca gaaaattttt atattatttt
      781 tacttctggt actactggag aaccaaaagg cgtacaaatc agtcatgata atctagtcag
      841 ctttgttcaa tggacgatgg aagactttgg gatcaagcct ggtatgcatt tcttagccca
      901 agcgccattt tcttttgatt tatcggtatt ttctatttat cctgcattag tttctggcgg
      961 aatgctgaag ccgttaaata aagcagttgt tcaagacttt cgtcaattat tcgcgacatt
     1021 gccagccttg aagttgaatg tgtgggtttc tactccatca tttatggata tttgcttgat
     1081 ggaacctaca ttcaatggag aaaatgtgcc ggaattggac atgtttttat tttgcggaga
     1141 agaattgacg aaaaaaactg cagaaagtct acttgatcga ttcccaaatg cacggatata
     1201 taatacttac ggtccaacag aagcaactgt agctatttca agtattcaga ttgaccagca
     1261 tgtactagat agttatgacc gactaccgat tggctacgta aaaaaagaca cgaaagtttc
     1321 tattgtccaa gaaggtaaac ctgtttcaaa aggagaaacg ggtgaaatca ttattgcagg
     1381 accaagcgta tcaaaaggtt acctgaacaa tcccgaaaag acagctgctg cgttcttttg
     1441 tcaaaatgac ttagcaagtt accatacagg ggatgcaggt agactagatg aatatgggct
     1501 tttattttat gaaggacgta tggatcagca ggtcaaatta catggatacc ggattgaact
     1561 cggggatatc gaacattatc tgctccaaga caaccgaatc aaacaagctg tcgtcgtacc
     1621 aaaataccaa ggaacaaaag tacagcagct ggttgctttt gtcgtattag aaaacaaaac
     1681 gaaacagccg gattttcagt tgactaaatc tatcaaagaa caattactag aagttgtaat
     1741 ggattatatg attcctcaga agttccatta tgtcgatctg ctgcctcaga cagttaatgg
     1801 aaaaatcgat cgaaaaaaat taacagctga ggtgaatcct gaatgatgtt acctcatatg
     1861 atcccttatg cagagccgtt ttattttttt cttttagcca ttgcgcttgt accgatcgtt
     1921 ctatctttat tgatatggga aaaaagatta ccagtttatc aatcagcagt gacacttttc
     1981 tttttatttg tcagctttgg aggtaattat tggcaacagg gagcggcgct gattgggtat
     2041 gttttgtggc agacgatact tgtatggggc tattttgttt atcggcaaag gaaaaatcag
     2101 acaggatggt tttttgccgc cgttatttta gcgataattc cactgttttt aacgaaagtg
     2161 actccttttt ttgaacatgg aaaagcatcg ttacttggtt ttttaggaat ctcttattta
     2221 acctttaaat ctgtacaaat gatcatggaa ataagagatg gcatgatcaa aacatataag
     2281 ccatttcgtt atatccagtt tctgctgttc tttccaacaa tctcttctgg accaatcgat
     2341 cgctatagga gatttgaaaa agatcagctc catccgcctg ttcgtgataa atatgttgat
     2401 cttttgggaa aaggcgtcca aaatattttt gtcggttttt tatataaatt cattatcggt
     2461 tattattttg gtcaagtact gcttcctgta gtgggacgca ttgccttaca gcatggtggt
     2521 atttcatggg cattagtagg ttatatgtac gtgtacagta tgtatttgtt ttttgatttt
     2581 gctggctata gcttatttgc agtcggtaca agctacatga tgggatatca gacaccaatc
     2641 aatttcaata aacctttttt atcatggaat atcaaagaat tctggaatcg ttggcatatg
     2701 acgctgtcct tttggttccg tgattatgta tatatgcgat tgatgttctt catgatgaag
     2761 aaaaaatttt ttaaaagcaa gatcgtaatg tcaaatatcg gttattttgc tttgtttctt
     2821 ctaatgggta tatggcatgg attgacgtgg tactacatcg tatatggact gtatcatgct
     2881 tgtctgatct gtctgacaga tgcatggatc cgatttaaga agaagcataa agagcagttg
     2941 ccttctaaca aatttacgca tggcttagcc gtatttttga catttcaagc agtatgtttt
     3001 agcttcctga ttttttcagg atttttagat aaactttttt ttagctaacg aatagtgaca
     3061 tattaattta tataacaaag gagaaaagga aaatggaaga acaaatttta tcaatcttag
     3121 aagaaataac aggaacagat gaagtaagaa aaaatcgtga tattgatcta tttgatgaag
     3181 ggttgcttga ttctttagca tctgttcaat tattagtcga gctagacgga gagctggata
     3241 tccaagtccc tgtatctgaa ttcgagcgag aggattggag cacaccgaat aaaatcattc
     3301 aacaagcaac tgcattgaag ggttgagtat gaaaaagaaa ctttttgcta ttttcggccc
     3361 ggttgtttta gctgttggct tactgtttgt gttttttacc tcctcctatc aagtagatat
     3421 ctctaatcca gaaacgatta gacgtgcctc ttcttccatg tcagtgaacg tgttgaaagg
     3481 agatgcgata aaaaattatg cattatctga aaaacaatat attccttttt ttggctcaag
     3541 tgaattaagc agaatcagtc catttcatcc gtctgttctg gcagaaaaat accagcgaaa
     3601 ctatcgtcca tttttgctgg gagcacccgg gacacaatcg ttaagccaat atatgatgat
     3661 gcgttctgca ggagatgcta tgaaaaacaa gaaagtcgtc tttattattt ctcctcagtg
     3721 gttcgttaaa aatggtgtaa aaactgatta ctttaatacg tattattctg aacttcaaac
     3781 atacgactgg ttgttttcaa tgaagaaagt aacgcctgct gatcgctatt tagctagaag
     3841 attattaact ttctcaaaag taaaagagaa tgatacgttg acggcgattc tgcaaacgat
     3901 caaaaaaggg aagctacctt tacctgaatc tttgaatcag ctgcgcagcc aatggaacat
     3961 gctgaagaga gaggatgaag tattcagcaa tattgggttg aaagatcgac agcaaaaaat
     4021 cgatcatgag tcaaaacgat taccaaaaca ataccaagaa acagaattat ccattttagc
     4081 aaatcaaatt ggagaacgag aaacgacgaa taatccattc gggttgaaaa acgattttta
     4141 tacgcatcgt atccgagccc acgagccaga gctcaaacag tcacaaaaaa attgggatta
     4201 tcgtttttcg ccagaatttt ctgattttca gctggtcttg gatcaattag caaaaaacca
     4261 taacgaagta ttgttcatca tcccaccggt taacgagaaa tggagcgatt ataccggact
     4321 atctcaagaa atgttacaag gatttgccaa aaagatcaaa tttcaattaa atagccaagg
     4381 attcaatcga atagctgatt ttgtcaatca agcgggtacc aactatttta tggaagacac
     4441 gatccatcta ggctggaaag gctggttggc tgcagatcaa cagatcagac cgtttttaga
     4501 agaaaatcat attactgcta gtaagtatca tcttgacgat gcttttttta gcaaatcttg
     4561 gcaacatcaa ataccagata aactacaatt aaaataaaga aaacaggcga gtgttgctcg
     4621 cttgttttct ttatttttga atattctgaa taaaagataa aaataccaag tttgcctgaa
     4681 caaaaatcat aagcaatggt ataatcacaa tatgaatata gaaatggggg aaaacatttg
     4741 gatacaaaag aagaacagct acttattgat ttaacttctg caaaaggagt acctggaaat
     4801 gaagaagagg taagagaagt attcagagaa tatgccaaac cattcgctga tgatattttt
     4861 tatgacggtt taggcagtgt gattgccaaa catggtgctg gcggaggccc caaggtcttt
     4921 atttctggac atatggacga agttggtttt atggtaacaa aaataaccga aaaaggattc
     4981 ttggaattcc aaactcttgg aggttggtgg gggcaagtca tgcttgctca gcaagttgaa
     5041 atcaaaacac gtgaagggaa aatcgtccac ggtgtgattg gttccaaacc accacatgtt
     5101 ttaacacctc aagtaagaaa taaaccttat gaaataaagg atatgttcat tgatattggc
     5161 gcatcaagcc aagaagaagc caaagagtgg ggcatacgtc ctggagatat ggtcactcca
     5221 tatattgaat acaaacgaat gaatggcagt aaatatttac tggcaaaagc atgggataat
     5281 cgtatcggga cagctgtttc tctgcgagta ttagagaatc tatctaaaga agcacaccca
     5341 aatgttttat tcgcaggaag cgatgtgcaa gaagaagtag gcttgcgagg tgctcgaaca
     5401 agtacacacc ttgtaaatcc cgatattgct tttgctttag atactggtac agcaggagac
     5461 actccaggaa tgacaccaaa agaagcagat tcgattttag gaaaaggccc acagattttg
     5521 atttttgatg catctatgat cccgcataaa aaattattga attttgttat tgatgtagca
     5581 gaggaaatgg atattccatt ccaatataca gtgatttctg gcggaggaac tgatgcagga
     5641 caaatgcatt tgacaagaga tggtgttccg tctgtagcca ttaccgttcc tgttcgttat
     5701 ctgcattccc atacatcagt gattcatgaa gatgattatt tgaatacagt gaaactagtc
     5761 acagaattag tgaaacggct tgatagtgaa acagttgaaa aaattcggag ttatgaataa
     5821 tgaataatag aatacctatt tctgaatatc ggaaaaaagc acgagtttct ttggaaaatc
     5881 aatggggaat caatacttgg cttgttttct tatctataat atttacgagc gtcatccaga
     5941 acttttttga cggtttcagt ccttttccgg aagaaagcat gcagcagcac ttattgagtt
     6001 ttctagtcga taatttactt ttattcgcat tcaactatgc attgttttac attgcgctag
     6061 tagttgtacg gggtggcaga ccaaaaagta accttttatt ttcagttttc caaagaaagt
     6121 attttggttc gatacttggc atcaatttga tcaattcagt catcaactgg ctactcaaca
     6181 gtttaatctt tctgcccatg tttttaatca gcggagtgag tacttatatg cagctgcttt
     6241 tcagttcaaa tgcggtacct acgggtgtca tgccagaaga ttttcttgat atcagctttg
     6301 ttttatcgat gatagggatg atttttgttt cagcactcat catgagtatt gttggcggtt
     6361 tattccaatt tgctgcatgg acaaagttcg actatcctga tttgtcggtt attcaatgtt
     6421 tgaaatatgg ctggttttta ttgaaagata gaattggtac gtatattttg ttgcagctgt
     6481 cttttatcgg ctggtatttg ctaggagcaa tcgctttgtt catcggtctt ttgtgggtca
     6541 ttgtttatgt gaatgtgtca gttgcagaat tttatgaaca agcacggatt gaaaaaggag
     6601 gtccagacgc ctattttact tagcatgtaa gcagtttatt catcaaaaaa ataacttttg
     6661 tggtaggata aatctttgag ggggaatgta cgatgaaatt aactgtttta ggatgcttag
     6721 gagcgtatcc ttataaaaat caagggacaa caggttattt gcttcaatct gacgggttca
     6781 atttgttgat cgatgcaggt agtgctacgc tgatcaaatt acaagaacac cttgatccac
     6841 ttgatctaga tgctgtgatc cttagccatt atcatcatga tcatattgct gaccttggtg
     6901 tattacagta ttactggcaa cttcatccta agagaaatcc ggagacattg ctttcaattt
     6961 atggacacac aaaagattcc attcactttg aagaattgac gttggaaaat gtctcaaaag
     7021 gacatcctta ttttgaagct gaagaattaa aaattggtcc ttttctgatt acttttatgg
     7081 aaacaatcca tccagtagta tgttacgcca tgcgtatcgt agaagaaaca acggggaaag
     7141 tattcgtatt tacaggtgat tcaggttatt tggaaagttt cgctgatttt gcgaaagatg
     7201 ctgatttgtt tctagcagat acctatcttt ttgaaggaaa tgaacatcat catgcccatt
     7261 tcacttcaaa agaagctgga gaacttgcga aatcagctaa cgtaaagaaa cttgtactta
     7321 cacacctacc tcagtttggt gatttagaac aattgaaaaa tgaagcgcaa aaggcagcag
     7381 gcaaagggat tgatatagaa cttgctgcag ttgacaaagt gtttaacatt taggaggaag
     7441 aacatgattt ttgtaccaaa tgaaaaccac gacccaagaa tcaatttagc aattgaacaa
     7501 tatttgctgc aagaaatgcc tttagatgaa cctattttgc tcttttatat caatgaacct
     7561 tcaatcatca tcggcagaaa ccaaaatacg cttgaagaaa tcaatcgaga ttatgtggaa
     7621 gaaaatggca ttcatgttgt ccgtcgctta agtggaggcg gagctgtcta ccatgatttt
     7681 ggaaacctga actttagttt tatcatgcct gatgatgggg attcattccg tgactttgcg
     7741 aaagtaacac agccaatcat ccaagcactt catgaattgg gtgttgctgg ggcggaattg
     7801 aaaggccgaa atgatctagt cattgatggc atgaaatttt ctggcaacgc gatgtatgct
     7861 accaacggtc ggatgtttgc tcatggaaca attatgttcg actctgatat caatgaagta
     7921 gtcaatgcat taaaagtaaa aaaagacaaa atcgaatcaa aaggaatcaa atcgatccgt
     7981 tctcgtgtaa cgaatatcaa accatttctt ccagaagaaa agcaaaaaat gacaacagaa
     8041 gaatttcgtc aagaaatctt gttgaaaatt ttcggcgtag agtcagtaga agaagtaaaa
     8101 acgtacgaat tgactgatga agattggaaa aaaatcaatc gaatctctga ggaatattat
     8161 cgaaattggg attggaatta cgggaagtct ccagatttca atttctctcg ccaaaaacgt
     8221 ttttctatcg gttcgattga agtccatttg aatgtctcag aaggaatgat caaagaagca
     8281 aaaatctttg gcgatttctt cggactagga gatatctctg acgtggaaaa aaaattggtt
     8341 ggacagaaat atgataaagc atctctatca aaaatagtca gccaaattga tgtgaagaaa
     8401 tattttggtg cgatagaacc agaagaattg cttaccttac tttattgaaa ataaaaaata
     8461 gcggaatcca catatatgga ttccgctatt ttttatacag gattcgggta atttcgagag
     8521 agttgaacag tgatccgatc aatgaaagta tctttttcct gcgttagtgt tcctgtgtaa
     8581 cgatccgcta cgtctttcat catcattaag tcaaaacgtt cctgatcatc taattcttct
     8641 gtgatgcctc gactaaatag gtttgtatcg taatgtgcac tatgttcgca acaaatgacg
     8701 aaatgttcct cttgttcgtg tatcgacaaa taaatttttc gatacagtgg attttcgatg
     8761 gcatgagttt ctctaaagga atgctctaaa gcgtggatca aaagttgagc cagatcgtca
     8821 tcgttgatag gaagttcttc gggaagatct gcttggaagg ttatctgaat attccgtttg
     8881 gcagctaact tctggtaacg cgctaagata agatttgtca cctggtggtt gcaaaaagaa
     8941 cacagcattt cgaaattttt tgcttcttct aatatctttt ccacatagtt tttagctttt
     9001 gtttggtccg tatgcattaa atcttgcaga ttctccatct cttggacaaa ttccaggcga
     9061 agtgtattta cttcttggat atgctgtcga agctgctcat aatgttgctc caacaaatca
     9121 tttttgattt ttaagaaatc tacttgtctc cgatctttgt cggtatttaa gatgtgatcg
     9181 attagattgt agctgataat aacgattaga atgataatat ataaaatcgt agaccaattg
     9241 atcgttgttt gatagatacc taatgccgtt tggatgtaaa tcaagcaatg gataatggct
     9301 accaagacga tccaaggtgt acaagctaca taaaaacggt tattcttata tgcttcgcca
     9361 atgcatgcaa gtgaagtgac taaagtgctt ataacactag caatattagc aatcggcatt
     9421 aattcaggaa gttctgcagg agtaaaagtt gcataaagca gaacgccgat aaaaatcaaa
     9481 agttggaacg tacaaaaaat tccgtaccat tttttatagt aaaacattcg gctccaatag
     9541 tagcagatga tgaaagctga cgttaagata ataaataaat gagataaaaa agaatgaacg
     9601 aatggttcaa aaagaatacc acttaaaata tcttctgata cagcttcaaa tcctaatgcc
     9661 aaagcaaagc aacttaagag aataagtgag tattcaagac gtttttgttt atacatcaag
     9721 tagatcgtat agaaaaaggt ccctatagcg ataaaaacag cgattatcat cgtcaatact
     9781 tgcggcactg attgtgaata aataaaacta atcaatggtc cagttgttcc tagataaact
     9841 ttcggtggca gtccagcata gttctgataa ggagatgaga ctgtaagggt caattttttt
     9901 ccgatataat ttgctggtaa atctatcaca gccaatgaga gtccgggatt gctttcttcc
     9961 tgataatctt tccgttggta taaaatcttt tcgcctagat atacagtaat ccattgatgg
    10021 tttcctttga ttagtaaatt tgcttcgtcc aacggttttt caagtgtcgt ttccataatg
    10081 aatgtttcgt ctttatccac ggtaggaatc gtacgagtgt acttgctgtc aaacttaaaa
    10141 tcaggatcat cttttgtata gaaaacccac gaatctttta tttctttcat ttgattgctg
    10201 tccgcaggag aaaaaataaa ataaaagaaa taagcaattc ctgttaaaaa taacaatgat
    10261 aatataatga ttatgctcca ctttatacgt aaataaaaag attctttttt attatatatg
    10321 ttattaaaaa aagacataaa ggtattcctt tctaaaactt
//
LOCUS       DAFEOM010000085        10157 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.088, whole genome shotgun sequence.
ACCESSION   DAFEOM010000085
VERSION     DAFEOM010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.088"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(561..1601)
                     /locus_tag="LP524_002418"
     CDS             complement(561..1601)
                     /locus_tag="LP524_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBL2235436.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1950..2276)
                     /locus_tag="LP524_002419"
     CDS             complement(1950..2276)
                     /locus_tag="LP524_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235437.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2263..3066)
                     /locus_tag="LP524_002420"
     CDS             complement(2263..3066)
                     /locus_tag="LP524_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBL2235438.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3419..3709)
                     /locus_tag="LP524_002421"
     CDS             complement(3419..3709)
                     /locus_tag="LP524_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HBL2235439.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(3845..4024)
                     /locus_tag="LP524_002422"
     CDS             complement(3845..4024)
                     /locus_tag="LP524_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235440.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4021..4371)
                     /locus_tag="LP524_002423"
     CDS             complement(4021..4371)
                     /locus_tag="LP524_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235441.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4364..5689)
                     /locus_tag="LP524_002424"
     CDS             complement(4364..5689)
                     /locus_tag="LP524_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBL2235442.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6139..6753
                     /locus_tag="LP524_002425"
     CDS             6139..6753
                     /locus_tag="LP524_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL2235443.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(6918..7922)
                     /locus_tag="LP524_002426"
     CDS             complement(6918..7922)
                     /locus_tag="LP524_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235444.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFYSKEKLADFSNIAEMIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLE
                     LKTLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITA
                     CEIGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEK
                     RNKLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNK
                     SQITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD
                     "
     gene            complement(8381..8638)
                     /locus_tag="LP524_002427"
     CDS             complement(8381..8638)
                     /locus_tag="LP524_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBL2235445.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(8631..8900)
                     /locus_tag="LP524_002428"
     CDS             complement(8631..8900)
                     /locus_tag="LP524_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBL2235446.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(9031..9210)
                     /locus_tag="LP524_002429"
     CDS             complement(9031..9210)
                     /locus_tag="LP524_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235447.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(9591..>10157)
                     /locus_tag="LP524_002430"
     CDS             complement(9591..>10157)
                     /locus_tag="LP524_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235448.1"
                     /translation="EEQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETE
                     KYIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATID
                     DYYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTY
                     TYDDTEEIQNELDRKRMPYQRFERKKRQ"
BASE COUNT         3101 a         1855 c         1458 g         3743 t
ORIGIN      
        1 tccgttgcgg gtaataccgt aacactgaag ggcacgcatt aaggcgtcat cttaatgctt
       61 tcctgtagga cactgacgga gattaccagt ctcccgagcc ttacaggggc ctttcttttt
      121 tttgtatttt caattatcac aatttatcgt ccaaaagtgc agaacgaaaa tcaattcatc
      181 acaagcatat cagacaatta aatttaaaac aactaaaaat atacaaaaaa gtgtaattga
      241 atattagcag cactgcctga aaagaagtaa tatcaggctt ttcagcttgg ggaaataagt
      301 gaattacctc tcttcatgaa ttattataac tcaaaaaagc acatatgcaa acactagttc
      361 cctatttttt tgaatgaaaa ataactaaaa tatctaacga ttatgacttt aaaacctacg
      421 tttgtgattg ttttcataaa cttttaatct aaaaactccg gaataatagc tatttatttg
      481 taacatttgt aataaaatta aaatcataaa aaagttctaa tttaaaattg atcattttta
      541 tagtgatcgc tttttatcaa ctactctagc caattttccg tactaactgt tggtaattcg
      601 ttaaatgttt ctctttgttc gtttgcaagc tttgccgcaa tagctttttc gacaaaccaa
      661 ttttttacat aaacgaaaat tagattctgc aaattgttta ctttttcagt tttttgtttt
      721 tgataagctt tcagcaaagt catatataat cctttgtcag catcaattcc ataagattcc
      781 agttcttcat aaacaacaac tgtttgctct aattcttcag cttttttctt tgcattatga
      841 atagtttgga cggttttttg agcgtcttcg taagtactag aaaatgtttt tatcaagttt
      901 aatacatttt ctggtacaaa tgtatttata ctgtcctctt tcatgatgtc gacgaagtta
      961 tctagtaaaa cttgattttg taactgatca gtttcagtgt ctattatgtg tctattagtg
     1021 tctatagaat tagaatagaa tagattgtct gcgattttcg caattacggt tgagtccttg
     1081 atatgactgg attttgccgt atttgcgatt ttcgcaatta cggtggtatc attgatttct
     1141 cgcggttttg ccgtatttgc gattttcgca attacggtgg tatcagtatt gtcttcctct
     1201 tccatgattt taaagacgtc ttgattcgtt acttctggct tgcctaagta caataaatta
     1261 ggcatggatt caatcttacc gtcacgttta tttactcttc ctctcttctg tttcagaaga
     1321 cctgcttttt ctaattcctt tttgatttta gatactttac cttcgcggca ttgtaacaat
     1381 ttcattaatt cttcaactgt gaatatgaaa aatatattat catcagaatc tacccagtta
     1441 tttttgactg aataatcaaa tcgatcttta agtagcatat aagcaatttt tgcatcattt
     1501 gatagatcca tgtatttttc gttagtaaaa aataccttag gcatttgata atatttttca
     1561 cgataaattt cttttgattt gtaataatta aaatcattca tagccaatcc tctttctgta
     1621 ggatagcgtt ttgttcaaaa ctatgttaca atgaattaaa caaatatatc tatccactat
     1681 atatttttct actgctgata agtcgccaaa ctttgttaaa gcagtagtct ttttttatct
     1741 ttttttagtt taccttaaaa aataaattaa agcaaaccgc tctatttcaa ggtttaagct
     1801 catagtaact atatcacgat attacaataa tattatatca cgatatcgta atattacaat
     1861 atgaagataa gcgacttatc agctttgata ctactgattt ttataaaaaa aatatcacga
     1921 tattatgata tcgtgatatt gaatgtaaat taaattttat tttgtttctt tgctagttta
     1981 tatccttcaa tcaagtattc ttcaggtatg gactttaata gcgcatcttc tataacgtct
     2041 ggtttacttt tgtctgattc agcaatagtc gtccaatatt tgagtgctga cattagaatt
     2101 ggatttatac gaatgctatc tgctgttctt acactacttc cttgatcttt tatgacttta
     2161 cttatatttt tcttggttaa ttcgatttct tctacttctt ttgatttaaa attcttttta
     2221 tttttatttc ctagtaatct acttactgca gcttgactgc gattatcttc tgccatcaaa
     2281 atcaccaact tcttttaaat attcttctgt aaatgcttta tagtcatctt cagctgttga
     2341 gggtttatta aaattgatta gatctgatcc agcagcttgc gcttcgtcaa cggttattgt
     2401 cctacgaata taagttttga aaatttttgc accaatgact ctagaaattt cggttgctgc
     2461 agacgtcatt tctttagaaa tgttataacg cttattatac ttagtaaata gaactcctcg
     2521 tacttcaagc ttgtcgttcg tatattcttt taccgtttca atagtttcac ttaactttat
     2581 tagtcctttt aatgagaaat acgcagcttc aactggaatg ataatttcat cagaaacagt
     2641 aaatgcgttg actgtaagca ttcctaaact tggtggacaa tcaattataa tataatcata
     2701 gttgtccttg attggttgaa gtactctttt aagcctgtgt tcacttccta cttgagtaaa
     2761 ttccctctct gcagaagaaa gggctatgtc ggctggcaaa acatcaattc ctttatagtc
     2821 cagaatagca tctgtaatgt cagcctcccc ttttaatact tcataaattg taggttggtt
     2881 tgttccatct atatcgaaac ctacattatc tgtagcattt ccctgaggat caatgtccac
     2941 taaaagtaca tttttaccgt aaacaaattt caaacatgct gctaaattaa cgctagtcgt
     3001 agttttagaa actccacctt tttgattggc tactgacaat attcttgcca ttaaaatatc
     3061 ccccattcat cttatattat aatataatta tatcataata ttgtgatatt gcaatatcgt
     3121 atatgcatta tattgtaata ttgtgtgatt attttgtttt aaagatatat atcggttcgg
     3181 atataaagat tattcatatg ttattcgaaa ttaaaataat cactttttat tactttaaaa
     3241 ttttttatta atgataagaa atatgttttg cgagcagcaa ctagaataat ttaaacatta
     3301 ctaaactgtc tatattgtta ctaataattc ccttcttata gtgcattttc ttagaaaaaa
     3361 acaatattac gatatcatga tattaagata tcgtaatatt atacaattgt gattgatatt
     3421 atatatgttt ttttattttt ttatcatgtt taatcttgaa ggaatcatat tctttttcta
     3481 aagacaggag cttttcataa acatcagaca tgtttaaact agaggccagg cttaaatcat
     3541 aaacgaatga agcaggtaat gattttattc tacgttgtcg ggttatccag cttgcaaccg
     3601 ttccttgtgg gtaggcatat aactcacaaa attgttctac agtgattcct aaattcttga
     3661 taatgtaaac attgatcggg tgcgagtaaa caaatgtatt gatagacatg gtgcgtaagc
     3721 ctactttctt ttattattgc gtttgcaata atagtttaac acacgcatta ttctagtatc
     3781 tactcctatt tgtaaaaaca aaaaaggatt catagagata actctataga atccgtaaaa
     3841 taccttatat atataacggg aagatataaa acctaaaatt atgataacct gctgcagctt
     3901 ctacatagaa agagaagcgc tcaacaccaa cctcatttat caaacgttgg ccataacccc
     3961 aaatctcttc tgatagctga tcaaaggtta tatactttag ctcatattgc tggataatgt
     4021 tcattctaat cgactccatt tattatcctg gtaaaattcg atatgacgta tttcgtcgta
     4081 gtgaattttg tcttttccaa cgtagatccc cagctcatca ctacctaaga tcttgccaat
     4141 gatatcttct ggatagtatc cttcagcgtt tcgttcttct ttttgaatag atactgatct
     4201 attttttatg actgcagcgt tcaatacttc cgcaatctct tcagtcgtca tttgttcttt
     4261 tgctaaattt tgactggttc gttgttcatt gtcggcatca atttcttcag tatgatcaga
     4321 taaataaaac cctgcccact ttaaacgtcc acgatcattg taatcaatca tttttaattc
     4381 ccccatttcc gcccgcatga ccaccaacaa gtgtgcttct agtaatgctt ctagcgcctt
     4441 ctaacattga agaagcatgt acaatcgacg taaaaccata tttttgccga atcttgtcaa
     4501 taattttatc taaattttct tcgtcaattt gcttttgtgg atcactaaat aaatccagct
     4561 gaagcgaatc ggtgtacaca agcttgctat aggttattcc aacatgcctt acttcttgtc
     4621 cttcataata cttatcaaac aagaaaagac aataattctg caattccttt gtattatctg
     4681 tgatagggat cttcatctgg tgtgaaaacc ctggtctcgt ttctaagatc gacgtgccaa
     4741 tgtgtaaatg gacacaacct gttttacagt tatgctgcct aatgcgtatc gctacttgtt
     4801 ccgccatttc ttttaccacc agctcgattt catttcttct agtataatct cgtggtaaca
     4861 cttgtgaatt tccatatgat ttttcctttg tcggttctgg tgggattgct atatctgttc
     4921 gatcgatccc attagcatgg aagtacagct gtaagccaat cacacctagt ctattcttaa
     4981 tcgtataagg attccagtta gcaagatctc taatgctcat aatccccatc tgattgagtc
     5041 tttttttcat tcgagagcca attccccaaa agtccgtcat ttcaggaata ttccatactt
     5101 tttcaggaac gttttcgtac gtccattccg caacaaatcc ttcattatgc tttgcttcgt
     5161 tgtctaaggc tagtttagcc aaaaggggat tatctccgac acctactgta gcaatcaatc
     5221 ctagctcttc tttaatattc tcctggatca tttgagccag cttctttctc cgttgacttc
     5281 gtgttccttc agtcgtaaaa agattcagtg atcgggtcac ttttaagatt gattcatcga
     5341 tcgagtaaat cagtagatct tcatcagcca catatcttct gaaaatatta tttacctgca
     5401 tatttcgctt gatatatagg ttcatacgtg gtggaacaac gtgtagtgtt ttaggaaatg
     5461 gttgtggtaa gtcacgtggt ctactcacat ttgtaatacc ataccgcttt tttgcttcag
     5521 gagaagaagc taatatcaat cctgaaccag tattatcagc tcgactcata acaacaagtt
     5581 ctgttgttaa tggatctaaa tttctttcta tacattcgac acttgcgtaa aagcttttca
     5641 tatcgattaa aaagtaatca tttactggct cttttgaata atccatcata aataacactc
     5701 cattctttca cattatacaa acatatgttc gatattgtaa agaagaaata gctaaataca
     5761 taaaaaaaga agcataggta tgctatcacc ctgcttcttc ttttgatttg tatgatcaat
     5821 ttattctatc actttactaa ttaatttgaa aatctaataa aaactctcac tctaaaagag
     5881 tgagagactg caaattagag tacggacttt tttggagttc cgttctttaa tattaacaaa
     5941 tgaataaaat ccttacaaca tttgagaata agttatcagt agacacttac aaaaaattct
     6001 catactattt cctccatcat tagtttttgt ctatataagg tttaccctaa atagacactg
     6061 gtataacatt gaaaaatatg gcttcctcgt gtatgattag ggtgtatttt aaatagttgc
     6121 agaaagtagg taaaagtttt gagagttgca tacatacgtg tctcatcgat cgatcaaaat
     6181 gaacaacgcc agattgaaga aatgaaaaaa ttcggtgcag agagaatttt catcgagaaa
     6241 caatctggcg caacgattac tcatcgacct gttttccaag aagcattaga ttacgtccga
     6301 gatcaagata tttttattgt ggaagctatt gatcgacttg gaagaaatta tgacgagatc
     6361 atcgcttcag tcaattactt gaagaagaaa aatgtgaagc tgatcattac tagtttaccg
     6421 atcatggctg aagcaattgg caatcctctc ctggataaat tcattaagga tctgatcatc
     6481 caaattttgg ccatgatcgc tgaacaagaa cggactgaat cgaaacgtcg ccaggcacaa
     6541 ggaattaaga tcgcaaaagc aaacggcgtc tacaaagggc ggcccaaatt atacagtgca
     6601 gacgcaaaag atccccaacg tcgccttgtt tacaaaagca tcgttgaaga tcttaaaaat
     6661 ggtgttgcga ttgctaagat tgctaaagac tacaatgtta cgagacaaac cgtgtataga
     6721 ataaaaaaag acagcatggt caatgacaaa tagttgatca tgctgctttt attatcttct
     6781 catctttaat ttcctacata taattactat ctataaaatt aagtcttact cagaacagat
     6841 aaagcataaa attccaacaa accatttttt acttaactaa ataaagagtc tgttaatata
     6901 atcttcactt atttacgtta atcccctact gtatttgttg gttccttatc tatattttct
     6961 acaaaatcct tattatcttg tgatttagct tcgtataatt ctttgtatgt cgacacattg
     7021 gttatactaa ttcctatagc tagttcttga gactctactt ttctctctag ctcctttgac
     7081 aaagtgattt gagatttatt tggttccgtt ggagtcaatt ctatagattc ttcataaaaa
     7141 tctctataca taaagctatc attaatttta taaaaaaaat taaatgtttt tagcactgcc
     7201 atataacatt caaaacattc ttctatagtt ggtttacttc cttgaggaga atgaaccaat
     7261 ttattacgtt tttctatgaa atttttaatt tcctttctta tttctttcgg aaactcaata
     7321 ttaaagtacc ctttaaacat gctccctaat aatttgtcta cagggggaga ttgtaaattc
     7381 tcaaatatta gagttaaatc aggtttctct tgttgataaa attcctttac ccctatttca
     7441 caggctgtaa ttatcgaaat aaacctatct ctagtatttg atatattttt acttctttta
     7501 aggagtataa gagaaaaggg gatactttcg tgtccatgca tgtaattaac taattgttga
     7561 gcttcagaag ttatatctct aattatatag ttaacggaat taggaagtgt ctttaactct
     7621 aatgaaattt ctatccaatt actatcttct aatctgtata tgctcgaagt ataatttgat
     7681 tcgtcagcaa gcatcgctac atcccaaaca ctgatcataa aatcttttaa acgttcaatc
     7741 atttcagcaa tattgctaaa atcagctaat ttttctttag aataaaaaaa aacatttata
     7801 tcagaatcaa taaattctat tttttttatt actatatcgt catcattatc ttgaccattt
     7861 atgtttattg ttccctgagt ggggagtact tctttcaaat tcaagttaaa tttatattcc
     7921 attacttctc ctccaatata taaaaattac ttaactgtat ccaaatttaa tatgtaaata
     7981 aatcattaat ttttagctaa attctatgag aagtttattt ttgttgtttt tgctaattaa
     8041 ctaaaactag tacaaaaaat ttaaaataga ttattaaacc tattgcttat tctattcagt
     8101 catatatata agcgtcatcc tttagtaact aacatcccac actacaatag tattataaca
     8161 tatctattca acttcaaatt gttgaaattt aagccattta tatttacttt tcctcaactt
     8221 tagaaatgat tttactaacc ttatatccgt aatacgcgta acaaacacat tcattaataa
     8281 caatcaacac taagctatat tagcttttta aatgactagg gtaaataaaa taaggatcaa
     8341 aaacttttaa gtttctgacc ctttccttac ttccgattgg ttaatagtga tcttttgcag
     8401 aataaataaa tatcgtttca ttttcaactc tatatatcag tctatgttca tctgtaattc
     8461 ttctggacca ttttccagat aaatcatgct ttaatggctc aggttttcct aatccagcaa
     8521 agggggaacg atcgatatct tttattaact tgttaatctt ttttatattg cttttgtttc
     8581 cttgctcatg ccaataaaga taatcatccc aagcatcatc agaccaagcc ttaatcatca
     8641 gattcaacct cgattaagtc atgtgtttta aatgcacctt tggagaattg ttcatctcct
     8701 cgacgaattt tttccatgac gtaattatta gaaagtgttc tcaacgtttc ttgcatagaa
     8761 tcataatctc tttttgataa tacaacaact gtatcttcta catctttact tgttacaata
     8821 agtgtttcag catcctcatt aacttgtttc atataactac gtaaattttg gcggaaattt
     8881 gaataagcta ctgcttccat tcctttcacc ccttatttat ttctataaaa caattgtaca
     8941 ttatattgta caattaagca aatgattaat attctaactt cttataatta aaagtgctat
     9001 cacttttagt ttttaggata aaaagtgacg tcactttttg agtgctggta cctcaaatcc
     9061 tgctttttct tgggcaatca ataaatctaa cgcaatacac ataatcctgg attcactttt
     9121 gccgtacttc tgctttagtt gctgcaattc tttttgtctc ttttcatcca acgtaacggg
     9181 taaccgttta tttcctattg caattggcat tagtactctc tcctttcatc gaaataaccc
     9241 tatgaaaaat atggctgcaa tcatcttgaa tagtagactc acaagggcta atacaaagcg
     9301 ccaaaagatc aaacaaaata ccagtattgc caaaaatact acgaagttta aatgcacagc
     9361 tgcagcatgg cgcataagta tttcgccaac aaataggact aacaatagag gaatgatttt
     9421 attcatatgg atctccttct ttctcttagg ttatcgggtg ttgcccgctt atttcgcacc
     9481 acctaagcgg cgggggcggt tggagcgatt ggtgcgaaat aagaaccatt cccttagtta
     9541 ctaggttttt tgtccttatt tcacacattc tcacttttcg gtataaatga ttattgcctt
     9601 tttttccttt caaatcgttg atatggcatt cgtttacggt caagttcatt ttggatttct
     9661 tctgtatcgt cgtaagtata ggtgtctaaa aaatcagtta aagtcgtgta ccagtcgtcg
     9721 cctgggttat ctacttcttc tacatattcc ctcaaaaatg cttcgggtac agcgagttca
     9781 taatccattc ccattaacac gatagattcc gttccacacg tcaaaaatct agtaaataca
     9841 aggtaataat cgtctatcgt cgcatctttg ctgtttagct cttccatttc ttcttccatt
     9901 aattccatgc tctcaaaaat tgaataatat tctgattctg agtaatagtg tttcctgcaa
     9961 atcgtgtagc tgacaaagtt ttcgaccagc tcactccttt ctaggtaaac attcatgatg
    10021 tatttttctg tttctagtcg tagaagctcc gttccttttt ttaccgtttc aggacgacgt
    10081 ttatacacgt ctaaacgatt atggctgact gtgaccttcg tttgactcgt ttcactttcc
    10141 aaatacaatt gttcttc
//
LOCUS       DAFEOM010000086        10145 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.089, whole genome shotgun sequence.
ACCESSION   DAFEOM010000086
VERSION     DAFEOM010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.089"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(41..406)
                     /locus_tag="LP524_002431"
                     /pseudo
     CDS             complement(41..406)
                     /locus_tag="LP524_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            complement(480..1130)
                     /locus_tag="LP524_002432"
                     /pseudo
     CDS             complement(480..1130)
                     /locus_tag="LP524_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            1455..1871
                     /locus_tag="LP524_002433"
     CDS             1455..1871
                     /locus_tag="LP524_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBL2235449.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            1868..2338
                     /locus_tag="LP524_002434"
     CDS             1868..2338
                     /locus_tag="LP524_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2235450.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            2352..3125
                     /locus_tag="LP524_002435"
     CDS             2352..3125
                     /locus_tag="LP524_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL2235451.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            3139..3951
                     /locus_tag="LP524_002436"
     CDS             3139..3951
                     /locus_tag="LP524_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL2235452.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            4005..4970
                     /locus_tag="LP524_002437"
     CDS             4005..4970
                     /locus_tag="LP524_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL2235453.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            5086..7809
                     /locus_tag="LP524_002438"
     CDS             5086..7809
                     /locus_tag="LP524_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL2235454.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANCMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            7822..8454
                     /locus_tag="LP524_002439"
     CDS             7822..8454
                     /locus_tag="LP524_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL2235455.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            9353..>10145
                     /locus_tag="LP524_002440"
     CDS             9353..>10145
                     /locus_tag="LP524_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2235456.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLY"
BASE COUNT         3398 a         1574 c         1961 g         3212 t
ORIGIN      
        1 gtgctttatc ggtcacaatt gctttcggct caccaaacta tttatggagt cgttttaaga
       61 aagcataggc tgcttgcgta tcccgtttct ttcgtaacca gatatctaag gttaagccgt
      121 ccgcttcaat tgcacgataa agataatgcc aacgtccctt aattttgata taggtttcgt
      181 ccattttcca tgaatagaag gattgtctat ttttcttctt ccaaagataa tagaggactt
      241 tgctgtactc ttgtacccaa cgataaatcg tagtatgaca aacatttatt ccacgatcat
      301 ataacaattc ctgaacttca cgatagctta gattgtaacg caggtagtaa ccaacagcga
      361 caataatgac gtcttttttg aattgtttgc ctttaaaatg attcattact ctgtcctctc
      421 tgtctttttt ctcaatttta cactaaaata gattttttgg aaaactttgc aacagaaccg
      481 ccaatcatat ggaagccacc gattaggtat tttatgggtt tagtcggaaa tgtttcaaga
      541 gcaatttcca tcatatcgat aatgccatga tgactacagc cagtaaagac aatcagacca
      601 tcttcttgct caattacgac caataattca tctgaaaaat catcataaac aaatttatca
      661 tccatctttt tgtataaatt cttggtggtc gagtttgctt tgcctagggg gatgatatgg
      721 aagctcggag caagttctgt tttcgttttt attgtatgta aattctcatg cattttaagg
      781 atttcatttt taggaatacc aacatacaga aacgtggtta aaatctttaa ataatggggt
      841 aaagttacac cttcagctgt ataaataggg gcgtttcctg attttaaata tcttaacccg
      901 ccggtgtggt cagaatgacc gtgtgaaata atcactgcct ccagatcatt caaagtttcc
      961 cccattttag tcgcattata gactaatttt ttattagcgc cagcatcgaa gagatatttc
     1021 ttccctgagt agttaattaa gagagataac ccatgtccac ttttaaagtt caaatttatt
     1081 cttgagtttt caaataaaac cttgatttcc actataacat ctcctttcac atgaacgtgt
     1141 attcatatat tgtagcacat taagttctat tttttgttgt aattaccgaa aaaaatatta
     1201 atggacgctt tttacgcttt atttcgtatg tggaagaaaa tttcttagga tgtggcgggg
     1261 gattggggca aagctaaatt ccaattacag tgatcattta attagacacc tttaaatacc
     1321 cacactttca tcaacactat aatttaggga aagaaaagtg ttggcatgat atagaaaaac
     1381 ctttaattaa cagcgtttcc taaaattggc acgcatcttg cttatagaaa agcgtaagga
     1441 taggaggaat gaaaatggca actataattc ttgccagcca tggggaattt gctcaaggat
     1501 taaagcaaac agcgacaatg attattggag atagtgtacc aattcatgcc ttgtcagcat
     1561 ttagagatga agatgaatct attttggtac aaataaaaaa acttttagcc acaatagata
     1621 tagaagaaac ctacatttta actgatattc taggtggtag tgtgaataat gatatgctca
     1681 ctattattaa agagtatgag ggattgcaat taattactgg aatgaactta cctttagtta
     1741 tttctattgc aacctataat ggaaaaattg gggaaagtga gctagaaaag cttattaaag
     1801 agagtcaaca atcagtgatt gattgtagaa aattattagt agaacaactt actggaggag
     1861 atgacctatg attaagttag tacgtattga ccatcgtttg ttgcacggac aggtagtatt
     1921 ttcttggagt aaatcattaa atatcaatcg tattattatt gccaataaag aggcagcgaa
     1981 tgatgaattc aaaaaaatgt ctttgaattt atctaaaccg actggcatta aattaacaat
     2041 tttttcagtt gaacagttac tagaaaaaat gcctaaaatc gaaacactta acgaaaatat
     2101 catgcttatt tttggtaata caaaagaaat tcgtcaattt tgtgaagcct atcctaaagt
     2161 acaagaaatc aactatggag ggataatcaa aaaagaaggt tccaagcaat ttagtaatgc
     2221 cattttcttg aatgaatctg agcaagaaga tgccaaaaaa ttaaaagctc ttggcattaa
     2281 acaatttatt caacaggtac caacttctaa aaaagaagat ttgaatacaa tgttataaag
     2341 gaggaatgaa aatgctcgtt cagtcaattg gtttagggtt aatcggtgtt ttttgtattt
     2401 tagactcaag gcttttagga agaatgaatt ttgaacgacc gttgattgtc agtacgcttg
     2461 ttggtttggt acttggagac atggaaaaag gattaattgt tggagcctct ttggaattga
     2521 tgtccttagg attagtaaat atcggagctg ctgcaccacc tgatatgaat atggcatcaa
     2581 ttattgctac agcctttgcc attttatcaa atgcgaatgc agaagcagca ttaacgattg
     2641 ccattccaat ttcagtgtta ggtcaaatac taggtatctt aatgcggacg attttgtcaa
     2701 atttgactca cgttgcggat cgctttatcg aacaagggaa atttagccaa gcaaggagaa
     2761 ttcatatact ttatggctcg ggattatatg ctctcatgta ttttattcct atttttctag
     2821 caatttattt tggaacgaat cttgtttctc agattgttga tgcgattcct gagtggctaa
     2881 caaatggctt aacggttgca agtaaaattt taccagcata tgggtttgcg ttgttattac
     2941 aaacgatgtt aacgaaaaaa atggtgccat ttttattact tggattttta attactgctt
     3001 attctggatt gggaatcatt ggtattgcag ccttcgcatg tattgttgcg tttgtcatgg
     3061 ttcaagttca aggtaaaaac tcaaatggta accaaaatga gccagatcca ttagatgact
     3121 tataaggagg tagattaagt gaaagagaaa gatcaaaatg catggaaata tcggcagttc
     3181 ttatggcgtt catgggcaat tcaagcctca tggaactatg aacgacaaat gaacatgggt
     3241 tttatgtacg gaattgcacc aattctagat aacatttaca agaatccaga agatgaagca
     3301 atgaagaaag aagcttacga gcggcatatg atgtactaca actgtacccc tcaaacaagt
     3361 gcatttgtgt tagggttaac tgcttctatg gaggagcaat actacgaaga ccaagaaaac
     3421 tttaatccgg aatcaatcag tgcagtgaag acttctctga tgggaccgct atctggtgtt
     3481 ggtgattctt tctttcaagg aacgattcgg gtattagcct ttggactagg aattaattta
     3541 gcccaacaag gtagtatttt aggtccaatt ctagcaatgt taatttcctt tgtaccttcc
     3601 tttttagtta cttactatgg tgggaaaatt ggctataaca tggggaataa atacttgtct
     3661 aaactgtatc aagaaggttt gatggataaa gtaatgtatg tttgctccat tgtggggttg
     3721 atggtcattg gttcgatgat tgccagcatg atcgggatta caacaccaat tcaaatgggt
     3781 aaagcttttg tcttacaaga tgtattagat agtattatgc cacaattttt accattagga
     3841 ttaaccttct tcatgtactg gttattgcag aaaaaggtca aaacaggatg gatgttggca
     3901 atctgtttag tcggaggaat tatttttagt ttgttaggta tcttacaata agggaataac
     3961 caataaattt ggttgaataa tataaataag gaggaattca attgatgaat tcaaaagaaa
     4021 ttgttaaaga aatcgtagcg aaaaaagagg taaaatcaat cgtatttgtt ggctgtggcg
     4081 cttcaaaagc ggacctttat cctgcaaagt attttctaga ccaaaatgcg aagcagttac
     4141 ggattagtca ttacacagcc agcgaattta actatgcaac accagcatct gtagatgagt
     4201 caactgtcgt tatttcagct tctttaggag gtacgactcc agaaacagtt gaagctaacg
     4261 cagtagctaa agaaaaaggt gctacagtta ttagtctaac acgaagcatt gactcaccgt
     4321 taacaaaaga tgcagattat gtaatttatc atcgatttgc agagaactat gctgctaaat
     4381 tagaaaaaac aggctactgt ttagaattgg cagtagaact tttacatcaa gtagaaggtt
     4441 atgaccatta tgaagcaatg caagaaggat ttgctactat ctatgatctt gcccaaaaaa
     4501 gtgcagaatc agctcgtaaa gatgcgaaga aatttgctga acgctttaaa gatgaaaaag
     4561 ttatttatgt aatgagtagt ggtgctacac atgaagtagc atactcaaca tcaatttgct
     4621 tgatgatgga aatgcaatgg attaattctg gtaccttcca ttcaggagaa tttttccacg
     4681 gaccattcga aattgtggat aaagacgtac cgtttatatt attaatgaat gatggaaaaa
     4741 ctagaaatat agatgctcga gcattaactt tccttgaacg ctttgatacg aaaacagaag
     4801 ttgtagatgc cttggattat ggcttgtctt cacatattgc taaagaagtc ttagactact
     4861 ttaacccatt cgtgattact gcagtattta gagtttatgc agaagaattg tcagaagcaa
     4921 gagaacatcc attaactaag agacgttata tgtggaaact aacttattaa aaaatagatc
     4981 aaactaaata aggtggcttg agaaattaag ccaccttgtt ttcttcaatg tgattttata
     5041 catatggtaa aataactagt acatattgtg aaagcaggtg gcaagatgca gcgattaaat
     5101 gactatcttt atcaactaac attgcttttg aaatctacgc cttcagataa ttattcagct
     5161 cgcagtattt cgcaagcttt acagttgaat agaagtacaa tcagtagcta cttaaatgaa
     5221 ggtgttcgtg aagggctttt cataaaagta aaaagttacc cggttttatt tttacataga
     5281 acagctcttg aagaactaca tatcacactg aataactccg aaatagaatc aattgaaagc
     5341 ttgttaacaa tttctcaaaa acccgcatta gatcaagtaa ttggctctaa aggaagtttg
     5401 aaagaagcaa ttgatcaaat taaaacggca gtcttatatc ctggaaaagg gttgccttta
     5461 ttattgatcg gcgctagtgg atcagggaaa acctttctag cgaataaaat atatgaatat
     5521 gcagtagaag aaaaggtcat tcacacatcg gcacctttca tagattataa ttgtgcacaa
     5581 tatgtgagca atccagagtt actatcctct gctttatttg gctacacgaa aggagcattt
     5641 accggagcaa gccaggagca tactggtttg ctagaaaaag ccgatggagg agtattgttt
     5701 ttagatgaag tgcatcgctt atctgaagaa gggcaagaga aattatttac atttatggat
     5761 acgggtgaat tctctcctat gggggataat agtattcgga aaaaagccga tgttcgatta
     5821 gtctttgcaa caacagaaaa tatatatacg actttcttac caacttttct taggagattg
     5881 ccagttattg ttaatctacc tagatttcaa caacgtccgt cgtttgaacg tctgtcattg
     5941 attgatgaat tttttgtttc tgagagtaaa attttagcta aggaactatc tgtttctgat
     6001 gcattgattc atttcttaat gaatagtgat tttgaaggta acgtggggaa tattaaaaat
     6061 attatcaagt atgcttgcgg aaacgcatat atccaccaga agagtcagca aacaattcaa
     6121 gttaaattat tggatatgcc tttagagtac agttataaat tcaaggagca actaaataaa
     6181 cccagaaaga aaagagcaga tcgaatctac tatccagaag aaacacgaca agtgcaacta
     6241 gaaagaaaca atcagctttt tgttcaattt tttgaccaat tacttactgg attctctgaa
     6301 gtgattgaaa ataacaagga tattaaacta tttttagaag aaatggttac caaggtaaca
     6361 caagtaatgg atacgcttat ttttcaagag gattacgaaa aagagcagtc tttatacgcc
     6421 attctaaact atcatattcg tcaaagtatt gattttatgc gagaaagcta cgggtttgaa
     6481 caagatggta accgggtagt cgctttggcc aactatctat atgtcaagga taatcaagtg
     6541 atttttgaaa atagtcttca agaaaagaaa accaaagatc agcttaacga attttttgct
     6601 aatgttcttg aaacccctta ttggtatgca aaaaaaatct taagcctttt atctgagtgt
     6661 ttagatcaac ctgtgtatga agaggacatt ttctttatta cgctatattt ttatagtttg
     6721 aatgtcacga atgttaaaac aggtattaag agtattgtgc tagctcatgg ttattctaca
     6781 gctagtagta tggcgaatgt tgccaactgt atgctgaata agaacgtttt tcaagcttat
     6841 gatatgcctt tagatattac attagagaaa attgaagcaa aattgattca attccttgga
     6901 gattatcgaa cagattcagg cttgatttta ttggtagata tgggttcact aaaccaacta
     6961 ggcggtttat tagtgaatca tctaaatgga ccactgttaa tcattgatca tatcaataca
     7021 ccgttattat tggaagtcgg ccaacaagta ttaaacaata aaagtatcac cgaaatagga
     7081 gcagaaattg aaacaagtct tactttcaaa aagcagttac tacttcccaa gaaaaagaaa
     7141 aagaaagcca tcattacttg ttgttataca gggattggta gtgcgaccca gattcaggag
     7201 attttaacga aatgcttgaa agatgcagtt agagaattag taattattcc gtatgattac
     7261 aaaaagctgt tagaaaacaa gaattttgag actccttttc aattatacga tgtcatcatg
     7321 attgtaggta cagaagatcc caaaattaat cagattccat atattggatt agatcgcctg
     7381 attaatgggg aagcaattgg cgaatttgtt cattacttgg aaaaagagct gactattgaa
     7441 gtgaacgatt tacaaaaaga attaattttc aatttttcaa ttagtaaaat tgtcgaaaat
     7501 ttaaccattt tggatccaga taaggtgcta tcttttgtac aaaaagcaat taaagaatta
     7561 tcgaagttat taaattttga tttttctaat aatcagctgt ttttgttgta ccttcactgt
     7621 agttgtatga ttgaacgaat tgtacgtaaa gaaagtgtgg atgagcaaga ggacattgaa
     7681 gaatatatgc aagcaaatgg agaaataatg gataaaattc atagcgcttt ttttgagatt
     7741 gaaagggaat acacaattga aataccagat ttagagttgc gattattgag cgatattgtt
     7801 caaggatagg agtgaattcg aatgacaatg ggagtaattt ttgatatgga tggggtactc
     7861 atagacagcg agacatttta ttttgagaga agaatgaact tctttcaaga actggagata
     7921 gatccaggat caaaccagat tgatgattat gtggggaaaa cagaagctgc aatatgggaa
     7981 acattagttc ctgaggacaa atcattaaga gaggaacttt atccgaaata tgtagattat
     8041 cgaaaaacgc atccaattga tttcgctaaa gatttacgaa aagaagtgaa accgttactt
     8101 gacttcctca agaagcggga tataccattg gcactggcat cttcttctcc agttgttgag
     8161 atagagagta tgctcattca atgtgaactt actgaatatt tttcctttgt tataagtggt
     8221 gagtcattaa aacaaagcaa accacatccc gaaatatatt taaaagcgaa acagcaatta
     8281 gactgtgact tctttattgc agtagaggat tcaacgattg gtattaaatc agcaaaggca
     8341 gcaaatatct atacggtggc actaaaacaa ccaatgcaaa tcgatcagag tgaagcagac
     8401 atacaaattg aaagtctaca agaactggaa caaattgttt tagaaaaagg ttagtgaatt
     8461 aatggaaata aggctatata tctgtgctag ctattttgac taaccaaaaa aacttgctat
     8521 aattcaggtg tagtaaaagg tgctggtaac accttttacg aaaatgcttc ggcagtgata
     8581 atgggattgg ctttttaagt tttttatttt gttcgcaatc cctaaggtca tgtgaatgat
     8641 cctagggatt gctagaatat tttgcaacat tctatctatt tcagaacgta accttcccca
     8701 gttcattggg attcattagc atacaaccaa catttcaaaa agaaattgca ctacaccatt
     8761 attagctatt ttgactaacc ccaaaaaact tgctataatt caggtgtagg taaaaggtgc
     8821 tggtatcacc ttttacgaaa ctgcttcggc agtggtaatg agtgtggctt aaaggactag
     8881 tcctttaagc tttttacttt gttcgcaatc tctaagatca agttaatgat cgataagatt
     8941 gctagaatat tttgcaacat tctatttcag aacgtaacct ttcccattcc actgtgattc
     9001 attggcaacc ctcctcacaa ccaacgtttc aaaagaaatt gtaaagttgt tggggtacca
     9061 cgaccgaaca atactacacc tgaatgatag taagttgtct cattaagtat agcatatcct
     9121 ctttttgggt aaatagaaat cggttacatt ttttatccaa gaccaagttt tttcatttgg
     9181 tcttatttgc tatacccaaa caattccgca gtatagtcga cggtattgat tttatcaggt
     9241 gagtaataga aacttgtttt ccttccccaa aacattacct ataaaggaga aaaaatggta
     9301 tcgtaattat ataggtatag gatgaaattt ttttctaaga ggagggttgt tgatgaaaaa
     9361 tttacagtta aaatttattg ttgatccatc gattgtacgc tggtttcgga ttatcaatca
     9421 atttgagcgc aatcaacttg ccacaaaagg agaattagca aagagtaatc atgtatctga
     9481 acgaaccatt caaacagatg tcaataaaat gaaggactac ttttctgaaa gtgttcaatt
     9541 tgtttcaacg aaaagtgggt attcttttca gaaggaaaga ccggttcttt ttctcaagca
     9601 gaaggagcaa ctactggaga acgaaatttt atttgagttg ttagggaatg tcttttatgg
     9661 cgaattggaa gatatgacgg agctaattga tcgttttcat ttatctgaag cgacttttcg
     9721 tcggtatttg aagcagattc aaccagtttt ggaagaatac ggcttagaat tgagtcttta
     9781 tcctcttgat ttaaaaggag aagaggcaaa tattcgcaag ttttttaaag acttctatta
     9841 tgaaggggag atgacgcccc atactcttgt tccaccaagg gatcttcatg aacttgtgca
     9901 agctacattc tatgataaat ttgagtattt ctcgattgga acagggactt ctcctgctga
     9961 attttattat agtttgtata ttgcgattga gcgtgtgaga caaggaaaaa cgatagccat
    10021 tcctcaaggt ctgattgagc gagtaattgc tagtgagaat tttcgcttaa tgtattcctt
    10081 gaaagaagcg attcaaacaa attatcaagt tgctttatca gaagaggagt tttgttggtt
    10141 gtatt
//
LOCUS       DAFEOM010000087         9672 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.090, whole genome shotgun sequence.
ACCESSION   DAFEOM010000087
VERSION     DAFEOM010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.090"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            711..2570
                     /gene="ltrA"
                     /locus_tag="LP524_002441"
     CDS             711..2570
                     /gene="ltrA"
                     /locus_tag="LP524_002441"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBL2235457.1"
                     /translation="MNKVKTNKTLRHSEYYGMTQIFDELYVKSDKKEVFTELMTIIMS
                     DENIRLAYRNIKNNKGSYTASTDKKSIRDIKTLSEDTFLTEVKVLFRCYKPRAVRRVE
                     IPKPNGKTRPLGIPSIWDRLIQQCILQVLEPICEAKFHNYSYGFRPNRSTHHAIATCN
                     RLANRGKLHFVVDVDIKGFFDNVNHSKLLRQMWTIGIRDKWLLGIIRSMLNAPIVLPT
                     GKVEHPKKGVPQGGVLSPLLSNIILNELDWWVASQWQNTTIETEKIMDRRDKGKGIDK
                     TNQYRALRRTNLKEIYIVRYADDFKIFCRKRSHADKIFHATKMWLKERLSLEISEEKS
                     KIVNLKKGKSEYLGFELKLIKKGKKYVVESHMSKKSMTRVKIQLKKQLRKVARPKNHC
                     EQAKEIGLYNSMVIGIHQYYGIATCVNLDCSKIAYTLKPFINHKLPTKKHGKILNTFI
                     KSKYGKSKEMRWLNNVPIVPLNYVQFRLAVPLSEGTCKYTESGRSKIHSKLKLDLALL
                     LHMMRNSNYERSIEFVDNRISKYSAQKGKCAITGKFLEYEEIHCHHIVPVKQQGTDKY
                     SNLIIIHKNVHALVHAIEEKIIHKYLNVLNLTNEQIEKLNELRVKAGNSVLTV"
     gene            2664..2903
                     /locus_tag="LP524_002442"
     CDS             2664..2903
                     /locus_tag="LP524_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HBL2235458.1"
                     /translation="MTSKDYLSLFNFRVALGRMSELGYSMMFGEVEKNFFMKHIKGRG
                     YVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            2981..3655
                     /locus_tag="LP524_002443"
     CDS             2981..3655
                     /locus_tag="LP524_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBL2235459.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            3648..3812
                     /locus_tag="LP524_002444"
     CDS             3648..3812
                     /locus_tag="LP524_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235460.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            3888..5030
                     /gene="dcm"
                     /locus_tag="LP524_002445"
                     /pseudo
     CDS             3888..5030
                     /gene="dcm"
                     /locus_tag="LP524_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            5020..5310
                     /locus_tag="LP524_002446"
     CDS             5020..5310
                     /locus_tag="LP524_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235461.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            5569..6753
                     /locus_tag="LP524_002447"
     CDS             5569..6753
                     /locus_tag="LP524_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBL2235462.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            6750..6887
                     /locus_tag="LP524_002448"
     CDS             6750..6887
                     /locus_tag="LP524_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBL2235463.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            7633..9543
                     /gene="ltrA"
                     /locus_tag="LP524_002449"
     CDS             7633..9543
                     /gene="ltrA"
                     /locus_tag="LP524_002449"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBL2235464.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         3479 a         1551 c         2059 g         2583 t
ORIGIN      
        1 aaaggttagg cacttaaaag aagaaaacat aagtttcctt ttaaggagaa ctgatatttt
       61 gagcagtgga atgatggggt aacgccctga aacgctccct taatactccg actggctgtt
      121 taacatatag ctgttagatg gtcagaagct cggtgaagtc ggctgaaagt taccgtaaat
      181 atgataaatt catatacgaa agttgttcag aggtgaatga tgtaacctat aggtcggagg
      241 tctataaaat actcacggtg agaatgtaag taatactact gacgaacttg cgaatgaaag
      301 aatctataca cggactgtat agaaatgtac aggattctac cgtatcaacg gaacagaatg
      361 tatgttgtgg tttagtaaga ttattggtta tgaacaacca tgcaatgtta caggcattgg
      421 aaatatacag gttcaaagca agcacctaaa agtatatata aagataaaaa tatcgaaaca
      481 tgggaagctg gcaatgtgga ggaaactata ttccattgaa acatttgtga cgaagaataa
      541 taagcacatt tatgccagtg aaagtggtgg cacagtacca atgaggatat agaaatatat
      601 cggaggaata gccactagtc aaaagaaatc gttaattaaa tgatgtattt catagaatcg
      661 agtaagacaa aataaacact acaacctaag taaacaaagg tagtggtgct atgaataaag
      721 taaaaacaaa taaaacactt cgtcatagtg aatactatgg catgacacaa atatttgatg
      781 aactgtatgt aaaaagtgat aaaaaagaag tttttacaga gctcatgaca attattatga
      841 gtgatgaaaa tattcgacta gcatacagaa atatcaagaa taacaagggc agttatacag
      901 cgagtaccga taagaaaagt attcgagaca ttaaaacact gtcagaagat acctttctaa
      961 ctgaggttaa agttcttttt cgctgttaca aaccacgagc agttagacgt gtagaaattc
     1021 ccaaaccaaa tggaaaaaca cgccctctgg gcattccatc aatctgggat aggttaatac
     1081 aacagtgtat tttacaagtc ttagaaccga tatgcgaagc taaatttcac aattatagtt
     1141 atggatttcg accaaaccgt tccacacatc atgcgattgc tacctgcaat cgactggcaa
     1201 atagggggaa gctacacttt gtagtagatg ttgatattaa aggattcttt gataatgtca
     1261 atcactctaa acttttgcgt caaatgtgga cgattggaat ccgtgataaa tggctattag
     1321 gtattattcg atccatgcta aatgctccta tagtcctacc aaccggtaaa gtcgaacacc
     1381 ctaaaaaagg tgtacctcaa ggtggtgtcc tatccccttt actatctaat atcatattga
     1441 atgaattaga ttggtgggtt gcttcacaat ggcaaaatac cacgattgag acagaaaaga
     1501 ttatggatag gcgagataaa ggcaaaggca ttgataagac taatcagtat cgggcattaa
     1561 gacgaacaaa cttaaaagaa atatacattg ttcggtatgc tgacgacttc aaaatctttt
     1621 gtagaaaacg aagtcatgca gataaaatat ttcacgcaac gaaaatgtgg cttaaagaac
     1681 ggttatcact tgaaattagc gaagaaaaat ccaaaattgt caatctcaaa aaaggtaaat
     1741 cagaatatct aggatttgaa ttaaagctaa tcaagaaagg taaaaaatac gtagtcgaaa
     1801 gtcatatgag caaaaaatca atgacaagag ttaaaatcca attaaagaaa caattacgta
     1861 aagttgccag accgaagaat cattgcgaac aagcaaaaga aattggatta tataattcta
     1921 tggtgattgg aatccatcaa tattatggaa ttgcaacctg tgtaaatctc gattgcagta
     1981 aaatagccta tacactaaaa ccgttcatca atcacaaact gccgacaaag aaacatggaa
     2041 agatattaaa tacatttatc aaatcgaagt atggaaaaag taaagaaatg cgatggttga
     2101 ataatgtgcc aattgtaccg ctcaactatg ttcagtttag acttgctgtc cccctatcag
     2161 agggaacttg caagtacact gaaagtggac gaagcaagat acacagtaaa ttaaaattag
     2221 acctagccct tttacttcac atgatgagaa atagtaatta tgaaagaagt attgaatttg
     2281 tggataacag aatatctaaa tattctgctc aaaagggaaa atgtgctatc acgggtaaat
     2341 ttctcgaata cgaagaaatc cattgccacc atatcgtacc agtaaaacaa cagggtacag
     2401 acaaatacag caacctcatt attattcaca aaaatgttca cgcattagtc catgctatcg
     2461 aagaaaaaat catacacaaa tacctgaatg tattaaatct aaccaatgaa caaatagaaa
     2521 aactaaatga attacgtgtc aaagctggta atagtgtttt aacagtttaa cttgaaaaat
     2581 atatcatact taacattctt ttgatggaac gccgtatgaa gtgaaagttt catgtacggt
     2641 gtgaagcggg ggaaaatctg gagatgacat caaaggatta cctatcgcta ttcaactttc
     2701 gcgtggcttt gggtcgtatg agtgaactcg gatattccat gatgtttggg gaggtcgaaa
     2761 aaaacttctt tatgaagcac atcaaaggtc gaggttatgt ggatacaggt ggcagtgtga
     2821 ttagtgagtt ttatacgcca cttgtaccaa aagggtatga tttcttagat tcaattagta
     2881 aattagaaaa gatgaatgat tgagtgatct tcaatactga ctttatactt caagtaacgt
     2941 ataatacttt tatatagtag gaagaaaggg gataattaat atggaaagta atgattctta
     3001 tttagagtat gcaaaagcaa ataaggataa gtttatttct tatattatca aagggaaatc
     3061 ccccgaagtt ggacgagacg cagtttttat ggcagggagt cctggggctg gaaaatcaga
     3121 ggttgccatt ggattagctg agaattatga taaccatgtg attattgatg cggattactt
     3181 tcgcacccag tttcctgatt ataatggaaa aaactctagt ctctttcaaa aagcatcttc
     3241 ttggttggta gagcaggcat ttagatatgt cttagatcat ggctattctt ttattctgga
     3301 tgcaactttt gcaatattaa gtgctgaaaa aaatatctcg cgggcgttga aaaaacattt
     3361 tcgagtgacg attttctatg tttatcaaga ccctaaaatc gcttggaaat ttacacaaga
     3421 acgtgaaatt gcagagggac gtcatgtgcc gaaaaaggca tttattaacg cttttttcaa
     3481 atcaagagag aacattgcaa aagtaaagag taaacatcca gatgtattgc ttcatatcat
     3541 aataaaagat tatacgaata atatttccga agttcattat gctacggata atctagaatt
     3601 atttttacca ctagggtata gtaggaaaga tttggaggtt caattacatg actgagaaag
     3661 aacaagtaac gaaaattgta aagaaataca ataagagcat tgcggattta tcggaaaatg
     3721 ctactgcaaa agagttcaaa acggtgatta aatatgtagc cgatcaggcc aatgaaaaac
     3781 aacggaaatt agttggatta aataaaaaat agtaggacaa attaataatg tatgagccaa
     3841 gagcaaaatg ttcttggctt ttttctttgc aatgaatgag gtaatgtatg agatttttag
     3901 atttattcgc cggtattggt ggctttcgtt taggtatgga acaggcgagc catcattgta
     3961 tcggtttttg tgaaattgat aaatttgcta gacgtagtta taaagcaatt catgatacaa
     4021 gtaaggaggt ggaaatgcat gacatcacaa gtgtatcaga tgagtttatt caatccctcg
     4081 gaccagtgga catcctttgc ggcggatttc cgtgccaagc tttttcaatt gcgggaaagc
     4141 ggcaaagatt ttccgatact cgaggtactt tattctttga aatcgctcgg ttcaccactc
     4201 ttctccgacc taagctttta ttccttgaga acgtccgagg attgctcaat cacgaaggag
     4261 gggctacgtt cgagacgatc ctccgaacgc tggatggatt ggggtatgac gtggagtggt
     4321 aggtgcttaa ctcaaaggcc tacattcccc aaagccggga gcggatattc cttatcggac
     4381 attctcgaga cgcatgtacc gaacaagtat ttcctatcat tggaccgtct tcaacatctg
     4441 atcaaaacat cagaaatttg gtaaatatca atccttccaa tcgaggaatg ggtggacaag
     4501 tctatggttc agatggcgta gcgccaacct taacaggtga tgaaggaatc aaaatcgcac
     4561 tgccagcaag ggatggaatc tctgtagcag gactattacc gggaaagttt gaacaaggta
     4621 atcgagttta tgaagttact ggaaccgccc ccaccttgtc aacgaaacaa ggtggtacaa
     4681 aaatcatgat tcgaaaaaat gaaacagaat atcaagaggt gaagccagaa gacagtgtga
     4741 atctggcttt tcctaattcc acaagtcgcc gaggtcgtct gggaaaacag agtgttcata
     4801 ccttactaac aggagaccaa caagcgatcg tcacggatca gtatcaaatt cggaagctaa
     4861 cacctagaga gtgttggcga cttcagggct ttcccgattg ggcttttgat cgagattctc
     4921 aagtcaattc agatagtcag ttgtataagc aagccggaaa ctctgtaaca gttccggtca
     4981 tttttgatat tgccagaaga ttaaaagagt tggatgtaca tggtttatga tgagttgatt
     5041 ggagaaattt attgggtgat tggaaagatt cagtcagacc cagaattaga agaacaattg
     5101 cgacggttaa aatttgatat tcgcaagaat ggtgtcaaag tcgctggcga tccatattta
     5161 atgaatgagg agacggatgc tcgaattgaa ataaatcaag taattgcaga atttgagcgg
     5221 attgcggacc aagcaaaaga accagatatt cgtcaatact tgttagaaat gaaagcagag
     5281 ttggaagtaa atggtattac agatgagtaa cctaaaccaa cacaactgac tgttaggcgc
     5341 tgaccgcgga ggattcgcga agcagaaatc ctccgtggtg ctgtgcctgt tcatggacgg
     5401 cgttagccga ccatgatgtc tcacccccgt atctaacagg ggggtacaaa aacacaaaaa
     5461 ataagtaagc aaccattaag aatgcttgtt gtatcaaaga tagtaagtaa tttgtatagg
     5521 tgttaactca tttgaaaaag ttaacacctt gaatttggag gtgagaactt ggcacaacgg
     5581 aatttagatt atcggctatt aaaagaccga cgcaacgaat acggcgtttc acaaaataaa
     5641 ttggctacgg cttgtggact tagtcggccg tacttaaatc agatagaaaa tggtggcgta
     5701 accgcatcta taaaaaccat gaggaaaatt tttgaccaac tggaaagctt taatcccgat
     5761 ttgcctttga cgttactgtt tgactatgta aggattcgct ttcccacaac ggatgcacga
     5821 aaaattattc aagaaattct ccatttgaaa tttgagtata tgctccatga agattatgcc
     5881 ttttactcct atcaagaaca gtatgtcatg ggtgatattg tggtgatgtt gtctcatgaa
     5941 gaagataaag gtgtgctttt agaattaaag gggcgaggtt gccgacaatt tgaaactttt
     6001 ttactcgctc aaaagcgtag ctggtacgac tttttcgagg attgtctaaa ggccggtggc
     6061 gtgatgaaac gcttggactt ggcaatcaat gatcgagtgg ggctattgga tatacctgag
     6121 ctaacgaaaa aatgtcagaa ggaagaatgt atctccttgt ttcgtacctt taaaagttat
     6181 cgttccgggg aacttttaaa ggctgatgaa aaggacggca tgggcaatac cttatatatc
     6241 ggcagtctca agagtgaggt ttatttctgt ttgtatgaaa aagactatga acaatatatc
     6301 aagttaggga ttccattaga ccaaactgag acgaaaaatc gttttgagat tcgactgaaa
     6361 aatgaccgtg cttttcatgc gattcaagat ttgttaaaag gtcgtagcat tgagagcacc
     6421 acgttttcca ttatcaatcg ttacttgcga tttgcggata aagtagaagg taaaaggcga
     6481 accaactggc ccttgaatga acaatggggg cgttttattg gacggaatcg gaaagagatt
     6541 caattaactt ccgagccaaa accgtatacc attgaacgaa ccttgaattg gctaggccga
     6601 caagtcgccc ctacgtggaa aatggctaag gagttggatc gattgaatcg gacaacctat
     6661 atccaggata tggtacagaa tgcacgctta tcagaccgac ataaaaagat tttggagcaa
     6721 caaagtatgg caattgaaaa tttgattata tgaaaagagg aatcgtcatg ggaattgtaa
     6781 aagatatttt tttggtattt ggtggcagta tgcttggtgt tactgtgatg tgcttgatga
     6841 atgcaagtgc agcagcggat cgagcaatgg aaaagaaagg aaagtgacaa gatgaatttc
     6901 ggacaaaatt tatgtgcgac tcgttcctta gtgaaaagct aaggcactaa taaaagaacg
     6961 aaaaagtttg gttattagga gaactgataa tctgacaggt ggaatgatga agtaacgctc
     7021 tgaaacgcct gccctgagcc tccgacaagc attgtatgag gaaactcaga atgtttgaag
     7081 ctcggtaaag acggctgaaa gataccgtaa tagttgatga acatatatct tctatcgaaa
     7141 actatccaga ggtgggtagc gtatccaaca ggtcgggggt ctataaaata tctatggtta
     7201 gaatgttcta taaggaactg acgaaacagc gaatgtacgg gtctaaattt gaactttgta
     7261 gaaatgcaaa ggtacgagaa atcgtagctg gtgtggcaaa gtaagaatat aggttatgaa
     7321 atgccatata acgttacagg cggtatcaag ccagcaggct tatagctagt gacctaagga
     7381 tatatatgta tagataagat tatcggaacg aggaaaggta ttgggttctc atcaagagaa
     7441 taaactgacg aagaacaata agcagttgaa ctaatgctga aaagcagagg gcgaacctaa
     7501 gaaattcttg taatgagaat ggaggaatgc cctcaagtcg tgtatatatt agaaaagtat
     7561 tatgttgttt caataaggat tacgagtaag ataaaaaagc tcactcaatt tcaagtaaag
     7621 gagtgaagcc ttatgcctaa aaataaaaat gaaacattgt gtgtagaaga tttaagacac
     7681 gctgaatatt acgaaatgca aaacaccttt gatgatttat atgccaaaag taagaatgga
     7741 gatattttca ctcatcttat ggatattatc ctatcgagag aaaatatatt actggcatat
     7801 cgaaatatca aagcaaatgc aggcagtaaa acagcaggaa cagacggtac aataattaaa
     7861 gatattggca aattgccagc agaaacagtt gtcaaaaaag taagatacat tgttgcaggc
     7921 agtccacatg ggtatcgacc taagccagtg aggcgaaaag aaataccaaa accaaatggt
     7981 aaaaccagac ctctaggtat tccttgtatg tgggacaggc ttatacaaca atgtataaaa
     8041 caagtattag aaccaatctg cgaagctaaa tttagtgaaa acagctatgg ttttagacct
     8101 aataggtcag ttgaaaatgc aataaaagca acctataacc gcctacaaat cagccagcta
     8161 cattatgtga ttgaatttga tattaagggt ttctttgaca acgtgaacca ttccaagctg
     8221 ataaaacaga tatgggctat gggaatacgg gacaagcact tgatattcat tttaaaaagg
     8281 atattaaaag ctccaatcaa aatgactaac ggtacaataa catatccaga gaaaggaact
     8341 ccgcaagggg gaataatatc cccactacta gcaaacattg ttcttaatga gttagaccat
     8401 tgggtagaaa gccaatggca ggaaaatcct gttacaaaaa actatgtggt tcatatcaat
     8461 aaaagcggta gcccatgtaa aagcaacgct tacaaagaaa tgaagaaaac aaagttaaag
     8521 gaaatgtata tggttcgtta tgcagatgat tttagagttt tctgccgcta caaggaaagt
     8581 gctgaaaaag ccaaaatagc aatcactcaa tggattgaac aacgattaaa attggaagta
     8641 tcacaagaaa aaacaagaat tgtaaatgtt agaaaaagat actccgactt tcttggtttc
     8701 aagattaaaa tgataccgag aagaaagaag ttagttgtta aatcacatat aagtgataaa
     8761 caattcaaaa accaaaaaga caagctggta tcacaggcaa agaaaattgc ttctccctca
     8821 aaagggaaaa cggaattaga agaaacaaga ctttataatt caatggtatt aggtatgcaa
     8881 aattattatc agattgcaac gaatgtaaat ctggattgta tgaaactgaa tagagctgta
     8941 atggctatct tcactaacag attaggtaca aacaaaaggg gcagattgag aaaaacaggc
     9001 agacaactta cgacaaagga ggcaacaaga tatggtaaat ctaaaatgct tcggtatgtt
     9061 gctggcgagg gcgaacctat tttccctgtt ggatatatcc aacacagaaa accaatggca
     9121 aaggtgtatt tagcgaattg ctacactcta gagggaagaa aatttatcca caccaatcta
     9181 agtgttgata aattactgat gtaccagtta atgaaattat ctttggaaaa tcgcacaata
     9241 gaatacgcag acaatcgaat ttcgctattt tctgctcaac atggaaaatg tgctataact
     9301 ctggaagaat ttcaacaagc taatgaaata cattgtcatc atattgtacc aactggagct
     9361 ggtggaaatg acgattataa aaatcttatt ttggtaaaag aggcggtaca ccgtctaata
     9421 cacgctaaaa cagaagaaac aatacaaaaa tatatagctc tattgaagct aaataaagtc
     9481 cagctcataa gactaaataa atatagagta ttagctggca atcaagaatt aaacttgata
     9541 taggtctact acataaatac ttttcaatat aaacacgatg gaacgcggag tgcggtgaaa
     9601 gtcgcacgct ccgtgtgaat ggggggaaaa gtttgagata tttataatat caggaactta
     9661 cctatccata ta
//
LOCUS       DAFEOM010000088         9447 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.091, whole genome shotgun sequence.
ACCESSION   DAFEOM010000088
VERSION     DAFEOM010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.091"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(42..680)
                     /gene="plsY"
                     /locus_tag="LP524_002450"
     CDS             complement(42..680)
                     /gene="plsY"
                     /locus_tag="LP524_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBL2235465.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            807..1241
                     /locus_tag="LP524_002451"
     CDS             807..1241
                     /locus_tag="LP524_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBL2235466.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            1407..3452
                     /gene="parE"
                     /locus_tag="LP524_002452"
     CDS             1407..3452
                     /gene="parE"
                     /locus_tag="LP524_002452"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBL2235467.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            3464..5914
                     /gene="parC"
                     /locus_tag="LP524_002453"
     CDS             3464..5914
                     /gene="parC"
                     /locus_tag="LP524_002453"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBL2235468.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            6191..8422
                     /gene="pflB"
                     /locus_tag="LP524_002454"
     CDS             6191..8422
                     /gene="pflB"
                     /locus_tag="LP524_002454"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBL2235469.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            8666..9427
                     /gene="pflA"
                     /locus_tag="LP524_002455"
     CDS             8666..9427
                     /gene="pflA"
                     /locus_tag="LP524_002455"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBL2235470.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
BASE COUNT         3336 a         1632 c         2046 g         2433 t
ORIGIN      
        1 tttgtcacac tcccgtttta tctctttgga atgaaaaagt atcaggattt tttcttgctg
       61 ctattcaaac caaacgggat tcgactttca gtatggtttc tgatacgctt gatattgtct
      121 ttatgccgat aaaagataaa ggaagaaact aaaaaggcaa tgatcgtcag taaccaattg
      181 ggcttagcca aaatcacagg agctacataa ggaaggacga tcgtagataa agtgatcaat
      241 actgcactga tcatactgct caaactcacc atacttgtac aaaataaact aacgataaag
      301 atgatacaag aataaacaaa aaatacagga ttaaatccta aaagcattcc tgcacttgtc
      361 gcaacagctt ttcctccttt gaacccagca aaaatcggaa atgtatggcc aagaatggcg
      421 cataccccaa accaaagagg gttaatccca gaaaggtgga agatcatcgg caaacaagta
      481 gccaatgtac cttttaaaat atccataagc aggacaacaa tcccggctgt tttgcctaat
      541 acacgaaatg tattggtcgt tccagtatta ccgctcccgt gttgtcggat atcttttttg
      601 aaaaacaatt tgccaatcca gacaccggaa ggaatagaac ctaaaagata tgctaaaata
      661 agtaataaga tcaatttcat tttttaccct ctttcaagtt gaaaactatc ctttatttta
      721 gcatgaatag gtaacaggaa caattgaaaa aatgaaagat acaagtataa tagatggaat
      781 aaaaagaaaa gcaaggggtg gcagaaatgt ctgtaaaaaa tccaacacaa gaaacgattt
      841 tcaactattt ggaagaagca aaaaatattg ccgtcgtagg cttgagcaat aaaccagaac
      901 gaacaagtta catggttgca caggtattgc aagacaatgg atataaaatc ataccagtaa
      961 atccattatt ggctggtgaa gaaattctag gcgagaaagt ctatgcgaat ttgacagaaa
     1021 tcgatgatcc aatcgatatc gtagatgtat ttagacggag tgaattttta cctgaaatag
     1081 caggtgattt tttgaaaact gatgctaaag tgttctgggc acaattaggg attgaaaaca
     1141 aagaagcagc tgagatgcta agaaaagcag gacgtacaga tatcgtaatg gatcgttgta
     1201 ttaagattga acttggtaag ttgaaagaaa accaaaaata aaaatgacca caaagaagag
     1261 atgatcgata gaggggaatg tcgaaaaagc ggttaggaga taattaaaat tgatttctcg
     1321 tctaccctct tttttttcac gaccttttct agtatgataa tcgtggtgcg gttttgccgc
     1381 aaagtattat tgaaggagcg tttgccttgg caaaaaaagt aaacaacgaa tacaatgact
     1441 cgtccatcca agtattggaa ggattagagg cagttagaaa aagaccaggg atgtacattg
     1501 gatccactga cagccgaggc ttgcatcatt tagtctatga aatcgtcgat aatgcagttg
     1561 acgaagcatt atcaggttat ggcagtgaga ttgatgtgac catccatgaa gacaatagta
     1621 taactgttgc agatagcgga cgtgggatgc ctgtaggaat gcatgcttcc ggaataccaa
     1681 cagttgaagt gatttttact gtgctgcatg ctggcggaaa gtttggtcaa ggagggtaca
     1741 aaacttctgg tggactgcac ggtgtaggag ccagtgtggt aaatgcctta tcgaaatggc
     1801 taattgtaac gatcgttaga gatggcgtag agtatcagca gaaatttaaa aacggcggta
     1861 aacctgacgg tactttgaaa aagataggaa aaacaaaaaa agcaaacggg acgactgtcc
     1921 attttttacc agatgataca atattcagta caacgaaatt ttcatatgaa attttagccg
     1981 aacggttaag agaatctgcc tttcttctaa aaggcgttaa aatctctctt tctgatcttc
     2041 gtggagaaga accagtcaag gaaatttttc attacgaaga aggaatcaaa gaatttgttg
     2101 attatctgaa tgaagaaaaa gatacattga caccagttgt ttatttttca ggagaaaaag
     2161 aaggtatcga agtagaggtc gcttatcaat acaatgacgg ttattcagaa aatgttttat
     2221 cttttgtcaa taacgttcgg acaaaagatg ggggaaccca tgaagctggt atgaaagcgg
     2281 cgatgacgaa atcttataat gaatatgcga gaaaagttgg tttgctgaaa gaacgggaca
     2341 agaatctgga aggtagtgat ttcagagaag ggttagctgc tgtactttct atccgtgtac
     2401 ctgaaaatct tctgcaattt gaagggcaaa caaaagaaaa acttggtacg ccagttgctc
     2461 gtaccgtagt agataacgtc atcagtgaac aaatgggctt ttatttgcag gaaaatagcg
     2521 aaatgagcca gatgttggtc cgtaaagcaa tcaaagccag agaagcgaga gaagcagcac
     2581 gtaaagccag agaagaaagc cgaaatggga agaagcgtaa aaaaggcgaa tctcttcttt
     2641 caggaaaatt gacacccgca caatcccgaa accctaagaa aaatgaattg tatttagtcg
     2701 aaggggattc tgctggcgga tctgctaaac aaggaagaga cagaaagttc caagcaattc
     2761 tacctttacg aggaaaagtc atcaataccg aaaaagctaa aatgcaagat atcttgaaaa
     2821 atgaagaaat caatacgatg atctatacga ttggtgcagg agtcggtcct gagttttcta
     2881 tcgaagactg taattacgac aaagtaatca tcatgaccga tgcggataca gacggcgccc
     2941 atattcaagt attactgctt actttctttt atcgttatat gaaaccgctg attgaagctg
     3001 ggaaagtata tatcgcgtta ccgcctttat ataaagtatc caaaggacaa ggaaagaagc
     3061 aagtcattga atatgcgtgg acagatgacg aattggcagc aatgattaaa aaagtcggca
     3121 aaggatatat gctgcagcga tataaaggac ttggagagat gaatgcggaa caattgtggg
     3181 aaacaacgat ggatccgact tcccgtacgt tgatccgcgt ccgtatcgat gatgctgcgc
     3241 aagctgaacg tcgagtaacc acactgatgg gtgacaaagt agaacctcga agaaaatgga
     3301 tcgaaaatca cgtgcaattt acgctcgaag aagatggcag tatcttagat aaaaaagagg
     3361 atactgaaat ctctccttca gtttctaatg atttattaga tgaagaacga gcagataaaa
     3421 atgaaaacaa tcagttattt gaagtagaat aggaggtcac ctgatggaaa atcgacaaga
     3481 agtacaagaa ttaactttag aagaagtgat gggtgaccgc ttcggacgtt attccaaata
     3541 tattatccaa gaacgtgcac tgcctgatat ccgtgatgga ttgaagccag tgcaacgccg
     3601 gatattattt gccatgaaca aagatggcaa tacgtatgac aaaggattcc gtaaatcagc
     3661 aaaatctgtc ggaaatatca tggggaatta tcatcctcat ggtgaccgca gtatttatga
     3721 agcgatggtc cgcatgagcc aagactggaa gttgcgggaa gtactgatcg aaatgcacgg
     3781 gaataacggg agtatggacg gtgatccacc tgctgcgatg cgttatactg aagccagatt
     3841 atccaagtta agtggagaga tgctagcaga tatcgagaaa gaaacagtcg atctcgtgtg
     3901 gaattttgat gatacagaaa aagaaccaac agtccttcca gctcgatacc caaaccttct
     3961 cgtcaatgga tcgacaggta tatctgctgg atatgcaaca gaaatcccga ctcataactt
     4021 agctgaagtg atcgatggca caatctatat gatcgatcat ccacaagcga gccttgaaaa
     4081 attgatggaa tatattcctg gacctgactt tccaacagga gggatcttgc aaggaaaaga
     4141 tgaaatcaaa aaagcttatg aaacaggacg aggaaaagtt attcttcgtt cgaaaacaaa
     4201 gatagaggca atgaagggta ataaacagca aatcgtgatc acagaaatcc catatgaagt
     4261 aaataaagcg accttggtga aaaaaatgga tgagatccgt ttgaataaga aaatcgatgg
     4321 aatagctgaa gtacgagatg aaagtgatag aacaggtctg caaattgtcg tcgaattgaa
     4381 aaaagacgcc aatgctcaag gaattttgaa ttatttgttc aaaaacactg agctacagat
     4441 caattacaat ttcaatatgg tcgctatcga ccatatgact ccgcatcaag ttggactgaa
     4501 agacatttta agaagttata tcgagcatcg aaagcaagtc ataacaaaac gtagtcaatt
     4561 cgatttagcc aaagctcaaa aaagacagca tatcgtagaa ggattgatga aagctttatc
     4621 gattctggat gaagtaatcg caacgattcg tgaaagtaaa gacaaaaaag acgccaaaaa
     4681 gaatttagtc gacgtctttc aatttacaga agaacaagca gaagcaattg tcacattgca
     4741 attataccgt ttgacgaaca cggatatcac agagcttcaa aaggaatccg aaagcttgat
     4801 tgcgcaaatc acagagttga ataaaatcct ttcaaatgac aaagagcttt tctctgttat
     4861 gaaaaaagaa ttgcgagaag tgaaaaaaaa ttattcttcc gctcgtctaa cgacaatcga
     4921 agacgaaatc gaagaaatca aaattgatac gcaagtctta gtggcacaag aagacgtaat
     4981 tgtcagtgtg acgcgagaag gctatgtgaa gcgaacaagc cttcgttctt atagtgcttc
     5041 caaaccagaa gaaatcggga tgcgagaagg cgactacttg ctatactcag gtgaattgag
     5101 tacattggac catgttcttc tgatcacaaa taaagcaaat gtgatctacc gccctgtcca
     5161 tgaactaccc gatttgaaat ggaaagatgc tggtgagcac atttctcaaa caatctccaa
     5221 tctgtctgta gacgaatcga ttttagctgt tttcccatat caaaaaattc aagcagagaa
     5281 aacatttgta ttcatcagca agaatggatt gatcaagcaa acacgaatga cagagttcga
     5341 accatggcgc acctataaga gccgtccgct cagctgtatg aagctaaagg cggaagaaga
     5401 tgaactgatt gctgtgtatc tgaaagaaga tcagccaggc ttagatgttt tcttagtaac
     5461 gcatcaagga atgggtttgc gctatccttt aacagatgtt cccgttgttg gagcaaaggc
     5521 agctggagtg aagtctatga atttgaaaga tggagatagg attatcaacg ggctgcttgt
     5581 gttagcagaa ggtgatacgc cgatcgtaat cgttactcag cgaggagctg ttaaacgcat
     5641 gcttgctcaa gaaatcagcc aaacttcacg tgcgaagcgg ggagtgacgg tcatgcgtga
     5701 gctgaaaaaa aatccgcacc gtattatcta tatgtcggaa ggaagatcaa aagaaataac
     5761 cttgatgaat caaaaagggc agcagttaac gattgaccca actgatttcc ctataggtga
     5821 tcgaacatcg aatggatcat ttgcgatgga tgagaaaaaa ggtggagaag tcatcgaagt
     5881 tatcgatgct cctgagattt tcatcgagtc gtaactgttg tagtatttta atgaagagac
     5941 gccatctgta ttaatacagg tggcgtctct ttttcattca tttttaataa atccttatac
     6001 gtttgcaaaa aaagatgaaa gtgttcacaa tgcttaaatg ttggtatgtc aacgttgatt
     6061 taaatatatg taaataaaac ataaaaattg ttaaataaaa aacaagaaaa cccttgcaaa
     6121 gatagggtgc tgtgttatac taatcacgta aaaactgtta cataaaacag aaatttagga
     6181 ggtccataca atggagcaat ggaatggatt taaaggtacc aaatggaaag aaggcgtaga
     6241 cactagagac tttattcaaa ataactacac tgaatatcgc ggtgatgaca gctttttgga
     6301 aggcccaaca gaagcaacag aaaaattatg ggatgaattg caaaagttaa atgatatcca
     6361 attcgagcgt aacggtgtct atgatatgga cacagaaatc gtatcaacta ttacttcaca
     6421 cgatgcaggc tatttggata aagatttgga aaaaatcgtc ggtctacaga cagacaaacc
     6481 tttgaaacaa gcattcatgc cgttcggcgg aatcaatatg gctaacaatg cgctaaaaag
     6541 caatggctat acaccaaacg atgaattgac acatattttc actgactggc gcaaaacaca
     6601 taatcaaggt gtatttgacg cttatactgc tgaaatgcgt gctgctcgta aaaacaaaat
     6661 cattacaggg cttccagacg cttacggtcg cggacgtatc atcggtgact atcgtcgtgt
     6721 ggcattgtat ggaatcgact atttgatgga acaaaagaaa aaagatcatg atatgactgg
     6781 ttatcataca atgagtgaag aagtgattcg tttacgtgaa gaaatcactg aacaatatcg
     6841 tgcattagcg caaatgaaag aaatggctgc gaaatatggc tatgatattt ctaaaccagc
     6901 tgcaaatgca aaagaagctg tccaatggct atacttcgga tatttagcag caatcaaatc
     6961 acaaaatggt gctgcgatgt caatcggacg tatctcagca ttccttgata tctatatcca
     7021 acgtgatttg gataatggag taatcacaga aaaagaagca caagaattga tcgaccactt
     7081 agtaatgaaa ttacgtatgg tcaaattcgc gcgtacacct gaatataacc aattattctc
     7141 aggattccct atctgggcta ctttatccat tgcaggtatg ggattagatg gtcgttcatt
     7201 agttacaaag aatgatttcc gtctgcttca tacattaaca aatatgggac cttcaccaga
     7261 accaaacttg actgttttgt attctgaaca ccttccagaa ggattcagaa catatgctag
     7321 cagaatcgct atcgaaagtt cttcgattca attcgaaaat gataatcagc ttcgtgaaca
     7381 atggggttct gatgattgtg cgattgcttg ctgtgtatca ggaacagttg tcggaaaaga
     7441 catgcaattc tttggcgctc gtgctaactt ggctaaaaca ctattatatg ccatcaatgg
     7501 cggagtcgat gaagtgactg gcgcccaagt tggaccagaa tatcgtccaa tcacatcaga
     7561 aatactagac tttgaagaag tgaaacataa cttcatgaat atgatggatt ggttagcaga
     7621 actatatgtc aatacgttga acatcattca ttatatgcat gataaatatt cttatgaagc
     7681 agctcagctg gctttgatgg attctgactt gcaacgcaca tttgctacag gtattgctgg
     7741 gatctctcat gcggcagaca gcttgtcagc gatcaaatat gcaaaagtaa aagcaatccg
     7801 taatgaaaaa ggaatagcag ttgattttga agtggatggc gacttcccta aatacggaaa
     7861 tgatgatgac cgtgcagatg aattagtttc atggatcttg gaatacttca tgggacaaat
     7921 caaacgtcat aaaacttacc gcaactcaaa accaacaact tcattattga cgattacttc
     7981 aaatgttgtt tacggtaaaa atacaggaaa tactcctgat ggccgtgaag ctggaaaacc
     8041 acttgcgcca ggagcaaatc caagttacaa tgcggaacaa agtgggttat tagctagcct
     8101 gaactcaaca gctaaaatga attacggctg tgcattggat ggaatttcaa atacccaaac
     8161 aatcaatcct tcaggtcttg gaaaagaaca agatacaaga atcgaaaact tgcgtaatgt
     8221 cttagatgga tacttctcaa aaggagctta tcatttgaat gtcaatgtat tcagtaatga
     8281 tttattgcgt gatgcaatgg aaaatccaat gaaatatccg aacttgacga tccgggtatc
     8341 tggttatgca gttaaattcc gtgacttgac aagagaacaa caattagatg tcttgatgag
     8401 aacttcacac gacagaatgt aaaaagttcg tgaaaaaagt ttttcgactt agcgtcgaaa
     8461 ggacgttgaa tagccatcgc atcaaaatcg aataatcagt atgcttattt gaaagaaacg
     8521 atttacaggg gcaggacttg tatgttccgt ccctgtaaat tttacacata acgaagcggc
     8581 aattgcgaaa aattttgttc agaaagcttc gcttcgacag ctaacttgta cttaaaatga
     8641 aatcaaagaa aagatgtgaa cttaaatgga agaaaaaaca atcggctatg ttcattctat
     8701 cgaaactttc ggttcagtag acggacctgg actgcgcttt gtcgtgttta tgcaaggatg
     8761 ccgcatgcgt tgccaatttt gtcacaatcc agatacttgg aatattggcg gagggaaaga
     8821 atacacagca gatgaacttt tagataaagc agaacgcttc agaccttact ggggagataa
     8881 aggtggtatt actgttagtg gtggagaacc tctattgcag atcgattttt tgatcgaact
     8941 atttaaaaaa gcaaaagagc ggaagatgca tactacattg gatacatgcg gaaaaccttt
     9001 tacgtatgaa gaacctttct tttcacgttt tcaagaattg atgaagtata cagatttact
     9061 gttatttgac atcaaacaca tcgataatga agagcataaa aaactaacgc attgggacaa
     9121 tgaaaatatt ttagaaatgg ctcaatattt gtcaaaaatc aacaaaccgg tttggattcg
     9181 ccatgtacta gttcctgagc gaagtgatta tgatgaatat ttgatccgac tagacaactt
     9241 tattcaaacc ttgtcaaatg tagacagagt agagattctt ccttatcata cgatgggaaa
     9301 atacaagtgg gaaacacttg gtttgaaata tccattagaa gggatcgaac cacctacaaa
     9361 agaacgtgtc gaaaatgcta aacgattgct tcatacagaa gagtatacaa aatatttaac
     9421 gcgataggtc gcatcggaaa gaagata
//
LOCUS       DAFEOM010000089         9444 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.092, whole genome shotgun sequence.
ACCESSION   DAFEOM010000089
VERSION     DAFEOM010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.092"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(130..972)
                     /locus_tag="LP524_002456"
     CDS             complement(130..972)
                     /locus_tag="LP524_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL2235471.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
     gene            complement(1009..2475)
                     /locus_tag="LP524_002457"
     CDS             complement(1009..2475)
                     /locus_tag="LP524_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016620473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL2235472.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            complement(2531..4375)
                     /locus_tag="LP524_002458"
     CDS             complement(2531..4375)
                     /locus_tag="LP524_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL2235473.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            4977..5927
                     /locus_tag="LP524_002459"
     CDS             4977..5927
                     /locus_tag="LP524_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL2235474.1"
                     /translation="MKNDIVTYANTKCEEIGISNLEECFFDTNDLSQFLRTGRTNASK
                     LANDLVAKNVLFKINTRPVLFAIKERFEEHFLIPVKDVYESISELKTDLEPNSTDSIL
                     QKMIGYDMSLSDAVEQMKTAVFYPGRGLPMMITGDTGVGKSFFVHTAYQFMKKVNVIH
                     ENAPFKVLNCAQYHNNPELLSGILFGYVKGAFTGADEDRNGLLQDADGGVLFLDEVHR
                     LPPEGQEKLFVYMDGGTYSRVGETIHHASNVRLVFATTEGKEDFLETFMRRIPIHIHI
                     PNIEERGILEKKQIIENTLFSESKNFSKAIDVSPKVTSVA"
     gene            complement(5972..6605)
                     /locus_tag="LP524_002460"
                     /pseudo
     CDS             complement(5972..6605)
                     /locus_tag="LP524_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(6865..7413)
                     /locus_tag="LP524_002461"
     CDS             complement(6865..7413)
                     /locus_tag="LP524_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235475.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(7414..8271)
                     /locus_tag="LP524_002462"
     CDS             complement(7414..8271)
                     /locus_tag="LP524_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBL2235476.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(8557..8844)
                     /locus_tag="LP524_002463"
     CDS             complement(8557..8844)
                     /locus_tag="LP524_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBL2235477.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(8834..9163)
                     /locus_tag="LP524_002464"
     CDS             complement(8834..9163)
                     /locus_tag="LP524_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL2235478.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(9240..9386)
                     /locus_tag="LP524_002465"
                     /pseudo
     CDS             complement(9240..9386)
                     /locus_tag="LP524_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
BASE COUNT         2848 a         1906 c         1444 g         3246 t
ORIGIN      
        1 gattttttat tatcttacgt taatcctttt taaatgtctc ttatagataa caaacagctg
       61 gaaatcacct tgtttttact ataatccatc tctaaagatt ttaaaagcat caatagtatt
      121 actgagcgct cacccattag catgtttcat caaccgattg atgtggacac ctaggtatac
      181 aacttcatcg ttcggaattg ttttatgata aactttttca atgtagtctt tgatcttagc
      241 tgcgctttcc atggcagacg ggtataagat ccacatttga ttgtagaatt cttcctcacc
      301 atcatccaac aaagcatcag aataatatct ttctacaaag aaacgtacat gtgtaatgaa
      361 gcgggcataa tggatcgaat tagtatcgat atctttctgc aacgaatacc ggaccaaatt
      421 gacgatcccg ccgatcattt ttgcatactt gaatccagcg ccgctatcag gatcttccga
      481 ttgtgcattg atcaaatgga aagcaatgtt tgaggcttcc tctttaggaa gatcaattcc
      541 atattgctcg ttcaaaaggg tgagtgcgta cttcccaatt tgaaattctt tgggatagta
      601 gatttttatt tcccagtaca aacggtttgc aatatttacc cctttttgtg cgcgttcaac
      661 tgcaaaatgc aggtggtctg ttaaggttaa atagactcca ggattcagtt ttttatctaa
      721 tcctttttct gcttcaaaaa cgatagtacg agtgatttca aaaaattcaa taggtatttc
      781 ttcaactaac tctaatagtt gtgaagattt cttttcttca aaaggcagaa atattttatc
      841 tactttatct cctgatattt catccccagc tttctgtcca aagcctatcc ctttacctaa
      901 tgcaatcatt tctttgccat tttcttcaac aagtaccaca cttgaattca atactttttt
      961 tattgaaatc actcattatc cccctatcgc tgcttaaaaa gatcgagact actcgaataa
     1021 ggatgcaccg tttgtttcga tcacttcttt gtaccagttg aacgaatcct ttttcaagcg
     1081 gttatatgtc ccatttcctt catcgtctgc atccacataa ataaatccat atcgtttaga
     1141 catttgagaa gttcctgcac taactaaatc aattggtccc cagctagtat aagctatcaa
     1201 atctacacct tcatcgattg cttctttcat ttgttggaag tgtgctttaa agtagtcgat
     1261 gcggtacgga tcatggattt taccatctac tacctgatcg actgctccca taccattttc
     1321 cacaatcatc aacggtactt ggtaacgatc ataaagttcg atcaacgaaa ttttcaaacc
     1381 ttttggatcg atttgccagc cccaatctgt tgaaggcaaa tgaggattct taacccctaa
     1441 aacagtattt cccggtgtgc gttctgcgtt tggatcagct gattctgcca tggacatgta
     1501 atagctgaaa gaaacaaaat ctacggtatg tttctttaaa atttctcgat cttcatcttc
     1561 cattttaatc tgaattcccc gattttccaa atccttttcg attagagcag gatattctcc
     1621 cttaacttga acatctgcat aaaaattatt ttctaagttc tttttcaagg tcagctcaac
     1681 gtcttctggt gcgcaagtta atggataagt catcaatttc gttaacatac aaccaacttg
     1741 gcttcctgga attttttcat gagcatcttt tgtcactaac gcactcgcaa caaattgatg
     1801 atgcaatgct tgatagatat cttgagcttc ttgaccttct gcgcttttct cttggataat
     1861 tccagctgtt gtaaatggat gacggtggat gctgtctatc tcattaaatg tcagccaata
     1921 ttttacgtat ttgccgaagc gatcaaaaca aacgttagcg aagcgaacaa aatcatcaat
     1981 tactcgacgc tccacccagc cattatattt aactgctaaa gctaatggca tttcataatg
     2041 agatagtgtc acaatcggct caattcctaa acgttgcatt tctttaaaga cgttttcata
     2101 aaatgcaacc cctttttcat tcggttccag ctcttctcct gtagggaaca agcgagtcca
     2161 agcaatagag aggcgcaagg ttttaaagcc catttcggca aataaagcta aatcttcttt
     2221 atagtgatga taaaaatcaa cgccccttct ttttgggtac catttatcag atttgtcttc
     2281 actagcttct tttatagact gcagggtgac atgcatatga gcagcaaaat ctttgttgtc
     2341 taaatttggt ttataagtag ccatatccgc tactgaaaga cctttaccgt ctacattcca
     2401 agcaccttct acttgatttg ctgctatcgc gccgccccat aaaaatcctt ttggaaaact
     2461 tgttgtgttt gtcattattc tattctcctc tattccttca ttcaatatat tttttttcac
     2521 tatcttgtgt ttaagctatc ggactgatag ctaagagatc actatcattg gttaccaatt
     2581 tttctaggtt aaatgaacgg atagtgtaat tatccttatt ggttaccaca cagataactg
     2641 tggggtcgaa tcctgcttct ttgattaaat caatatcaaa tgcaatcagc ttttgtcctt
     2701 tggctacttt ttcaccagaa cgaacaaatg cctcaaaccc ttttccttct aactgaacag
     2761 tatcaatacc aatatgaaaa agaagttctg caccattagc cgtttttaaa ccaatagcat
     2821 gcttcgtttc aaataccatt gtgatttctc catcttcagg agctactaat atcccttctt
     2881 caggaatgat tgctactcca tcacctacca taccactaga aaaaacagtg tcttttactt
     2941 ctgatagtga gattactttt ccattcacgg gacttgaaag aacagcatct gaaacaacgg
     3001 ccgtttccat cttagtttca acttcattga caacttcttt atctttataa agaaccaacg
     3061 ctgcagcaaa agcaaccaca aatgaaatcc ccgatactgc aaatgcccaa accagattca
     3121 ttgggtcagc tttgtcagcg aacatcgtga tacttgcaat cccaggaccc actaaagcaa
     3181 aggcttgaag tgcaacgatt cctgcaaatg ctcccccgat caggctagaa aacataacgg
     3241 catataaaac acgtttgtgc agaatcgtta ccccgtaaag agctggttca gtaattccga
     3301 ataatgcaga tatacccgca gaaacagccg tagatttcaa ttttttgtct tttgttttga
     3361 tactgacagc aaagcatgcg ccagcttctg aaatattgtg tgccaatgaa gccgggagat
     3421 aaagaagttc tgcacccaat tcactcatgg atgaaacagc gtaagggatc attgctttat
     3481 gcatacccgt tacaaccatt aatggcagta cagctgctaa gactccggtt gcaacccatc
     3541 caaattgatt gtacaaagac acaatgatag aagagaaagc tgttcctaca ttataaccta
     3601 atggtcctag aagcaacaaa gcaataggaa ctgtaatcaa taagctcatc ataggtacaa
     3661 agaaaatacg aattggtttt ggtgaaactt ttgtaaacca tttttcaagt tgagcataaa
     3721 ataaaacagt taagatggct gggaatactt gagatgcata cgctatatta tccaatccaa
     3781 aagataagaa tgtagcacct tccgccaaca tagctgtcat ttcaggaagg atcaatgcac
     3841 cgacagcaga tactgcaacc aattgattga ctttcaattt attagccgta gtcattgcca
     3901 ccagaatagg caagaaataa agcggtgccc cgccgaccaa atttaaaatc tgataggttt
     3961 gagattggtc actaatcagc cctaagatag aagctagtag caacaaggac ttcaatactc
     4021 ctccacctgc aatcgcagga attaaaggtt ggaaaataga aataataaaa tctaatagaa
     4081 ctgaacctaa tttttgtttt tgttttggtg cttcttcagt atttgaaaca ttgtcaccta
     4141 ataacgcttt cacttcatca tacacttcaa caacatcgtt tccaatgatt acttggcatt
     4201 gcacattttg gcgaacgccc ataacgccat ccactttttc taactggctc atatttacct
     4261 tgctgttgtc ttttaacgta aaacgtaaac gagttgagca atgctccaaa tgggatatgt
     4321 tttcttcccc accgatgtac tgcaatactt cttctgctgt ttgtttgtaa ttcatggtgt
     4381 ttccctcttt tcttttttga gtgatgaact tttgagtcga agtggccatc ctcaacacaa
     4441 aaaacaggca gaaactaaat aaattgacaa caaggcaaac gtgtattgaa acgcatacat
     4501 tgttattcaa cttatttagg tcctgcctgc attaccagtc acatgcctgt ttaagttata
     4561 ttcaatttaa catattagaa tgtaaacgta aataataaaa tgatttttta atcatttttt
     4621 cattttattc gttcaatata gttttcgtgt tctaaaagtt ttgtcttttt tctaagaata
     4681 tcttccaaca tatataactc ttattctctt ctatcttcta gcagatgttc tgtcttttta
     4741 agacctagta taaaaagaat gggttcattt tttctttctt gcaggatgag gttttttata
     4801 taaactttat ttgatataaa gaaaaggcat tcataattgt gctatactac taaaaaatga
     4861 taatacagtg aattagatta gcgctttatt aaattaagag gacagcatat cgatattgct
     4921 tccttttttt tgctatttat ttaagggata gaagtttaga aaggactgtt tataagatga
     4981 aaaatgatat agtgacttat gcaaacacaa aatgtgaaga aattggcatc agtaatttag
     5041 aagagtgctt ttttgacaca aatgacctgt cgcaattttt aagaacggga agaacaaatg
     5101 ccagtaaact tgccaatgac ttagttgcaa agaacgttct atttaaaatc aatactcgtc
     5161 cagtgttatt tgccatcaaa gagcgctttg aagagcactt tttgattccg gtaaaagatg
     5221 tatacgaaag tatatctgaa ttaaaaacag acttggagcc caattctaca gattccattc
     5281 tacaaaagat gattgggtat gatatgagct tatccgatgc ggtggaacaa atgaaaactg
     5341 ctgtcttcta tcctggaaga gggcttccta tgatgatcac tggagatact ggagttggaa
     5401 aaagtttttt tgttcatact gcttatcagt ttatgaaaaa agtgaacgtt attcatgaga
     5461 atgctccttt taaagtactt aattgtgcgc aataccataa taatcctgaa ttattgtcgg
     5521 ggatactatt tggctatgtt aaaggtgctt ttaccggcgc agatgaagat cgaaatggat
     5581 tgttgcaaga tgcagacggt ggagtactgt ttttagacga agtacacaga ttgcctcctg
     5641 aagggcaaga gaaactattt gtttatatgg atggcggaac ttattctaga gtcggtgaaa
     5701 ctatccatca cgcctccaat gtacgtttag tttttgcgac tacagaaggc aaagaggatt
     5761 ttctggaaac ctttatgcga agaatcccta tacatatcca tatcccaaat attgaggaac
     5821 gaggtatttt agaaaaaaaa caaattatcg aaaatactct ctttagcgaa agcaaaaact
     5881 ttagcaaggc tattgatgtt tctcctaaag tcactagtgt tgcataaata tagtcagaat
     5941 attctgaatt attattttct tgaaaaaagt aaaaaaaatc gtatcccaaa ctttcatcta
     6001 catttccaga aagatcttca tgttgataat caattcctga atcaatgacc gctaccttca
     6061 catccttaga acccttggtt tcttcccaag cggatggcat gctaatctct cttaaattag
     6121 cttgtttact ccaattagga tcgttatcga tttcctaaag aatcagttgt atttggctcc
     6181 tcaacatcat tcaacggctc tagttcaacc acatagtttg gctctgcata attgaccata
     6241 tcagaagcat taaatgttct tactgcctct tccacactaa tttcttcgtt caatttcact
     6301 aataatactt gatctcgctt attataatcg ctttcatttg tcctaagagt caaatctttt
     6361 tccaccataa cttttacacc caataaattt tgcggtttat ttttctctgt tgaaattctt
     6421 tgtgtagcct ttaaactgac aattagctca ttgggtacat actctatttg agagtcatct
     6481 aattggctca taatcctttc tttttggtta gtatctgcag ttacttgttt agtatgagcc
     6541 atgaacccaa acatactcac cccaacaacc aatcctaaat atattttctt tttaaacttt
     6601 ttcatctaca tctcctccta atagttttat tttagagtta gctctattac ttatttacta
     6661 actcaacaac agattatcat acacttatta atgaaatcac aatatctcat taaaacacac
     6721 cctaaaatca taaccgttcc tatgctcatt ttttggattt tttaatcacg taaccagaaa
     6781 aataaatccc ctctatttca cgcaaatcaa agaagaacta agtaactacc gtttaaaggt
     6841 agttacttag ttcttctttg attattatct aactttatta ttcattcccc accctttgcg
     6901 cagtgagtct tcggtatctt gttttatctg actggtagtt cttattattt ctccacgatc
     6961 atctaattct ggaacaattg gatatttttc tctgaacaag tctagttctt tcaccacgtc
     7021 attaaacaaa tcttgtttat cttcttgcgt aagattctgt ttaatatact catctaaagc
     7081 ttttctaagc aattcatctc ttgtcatatt agtctcatac ttttctacta aaactctgga
     7141 agtcgcattt aacaaagtcg acagttttaa aggatgcttg gtggaataaa ccttatcaaa
     7201 ctttctgatt ggtgcgccaa ctttttttgt tcttcctcta ctctgtcttt caacattgga
     7261 ggaagttttt atttcttgaa cttggtgcac ttctaattgc ttattttcag atggtgtaat
     7321 tgcttgctgc tttttctttg cttctgcttc tttttttatt ttggcgagtc ctttgttgtt
     7381 cccaaaaata tcaagtcctt tgatgtcagc caattactcc acctccgcat accaattctc
     7441 gtgttcttcc aattctttca aaatactgat aaataattcg tggcttttgc gatcccattg
     7501 ttttaagaga cctttctttg tcttctccat cgtaatacct gatccgtcgt atcgttttaa
     7561 gcgcgcattt ttgagtacaa catttttaat caaatgttcc ccataaatct cttgggcttg
     7621 tttatacatt tcctgatcta ctgcgtcacc gctttccaac atgaaaggaa taacaccagc
     7681 aacatataaa tttgtatcaa attctttcac aaaaaagtcc atatactcaa gatattgcat
     7741 cgcatttctc atcgatttta cttgggtctg taagacgacg attgcccaat ccgcaatgta
     7801 catcgcggat ttactatata cagaaataga aggtgggaca tctaaataga tgacgtcaaa
     7861 ctcatcaaat aatggttcta gcttttcttt catatatttg aattgggata atgaatcatt
     7921 atattggtct ttgatccaat catcatacat ttcaaaggac atgtctgctg gaagtatata
     7981 gaggttattg attactttat agacagcctc tttaggtgga agattgttta tcaacacgtc
     8041 agtcacagta ttttcgtaaa caatcggttc accttctccg cctccttctt cgttagtgaa
     8101 gttcatatgc gttaagttca tcacatcaga agtatttcct tgcatatcca agtcaattat
     8161 caaggttctt tttccttcat taactgctga ttcaaaccct aacatttgcg cgcacgtact
     8221 tttacctacg ccacctttaa aattaccgat tacatatttc ttcgctacca cgttcctacc
     8281 tcttttcttt tagcaatatt catttgatac tattataaca tatttatttt tagttactac
     8341 caaaaaaaag atatatatgt actaccttat aattaataag taaaaggtag tacataaata
     8401 aggaaaagct gaaatatatt gatttatagc tttcctcaaa gtaatgacgt tatttaattt
     8461 atcgagaagc agagaatact cgcgacttta gtcatgtaga cgaatcagat tttcgataaa
     8521 aaagaagccg tccttcttaa gaacgacaac aaatcactag attaattttt tcaactgttc
     8581 ataaaagttt tcatgcgttc cacataacaa tactggaata accttgtttt ctataatctc
     8641 gtaagctact ctatatgttg tgcccgcata tttgaaccca ctagaccaaa tacctgataa
     8701 atccccagtt ttttgttcac ccttatatgg atcaatcgca atttcatcat aaattaaatt
     8761 caaaaatttt tctttcaatg gcttctcttt tagctttttt aaatacttaa tgacatgttt
     8821 tgaaggaacg atttcataag ccaatttctg ttgccaactc ctctttagtc attactttag
     8881 aattagtaag tgtatcttca gcaatatctc taaaggaaga atgcattttg ttttttctat
     8941 tcttaaattc tacaagaatc tcatctttat cgtagccttc tgcaattata tcagacaaaa
     9001 tatcttcgct aaaatcataa aattcttttt gtggttcaac aaattcataa agaataccat
     9061 tctctaccaa ctttgcttca acttcgactc cattaggaac attaaaatct ttaggtactg
     9121 tcaacatgat tgaattgcct tgttttcttg tttttgtaat catttaaatc cctcatttct
     9181 aacagtatgt aattacttag taaatacatt ataccacgag ataatgtatt tacaagtaat
     9241 tactgtgact cgtaatgcgt ccttgctgaa taaatttcaa ccacgtgttc atcttcatct
     9301 accttataaa caacccggtg ttgtctattc aatcttctaa aatacctacc ttcatgtgta
     9361 ggttttaact tctcaaatga ttgggttctg ttgcaaagtt ttaaataaag aataaaatcc
     9421 cttacggtat ctatgattta agct
//
LOCUS       DAFEOM010000090         9388 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.093, whole genome shotgun sequence.
ACCESSION   DAFEOM010000090
VERSION     DAFEOM010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.093"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            2..77
                     /locus_tag="LP524_002466"
     tRNA            2..77
                     /locus_tag="LP524_002466"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            79..149
                     /locus_tag="LP524_002467"
     tRNA            79..149
                     /locus_tag="LP524_002467"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Gly,seq:tcc)
     gene            163..236
                     /locus_tag="LP524_002468"
     tRNA            163..236
                     /locus_tag="LP524_002468"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:197..199,aa:Ile,seq:gat)
     gene            261..348
                     /locus_tag="LP524_002469"
     tRNA            261..348
                     /locus_tag="LP524_002469"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:295..297,aa:Ser,seq:gct)
     gene            370..441
                     /locus_tag="LP524_002470"
     tRNA            370..441
                     /locus_tag="LP524_002470"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:403..405,aa:Glu,seq:ttc)
     gene            464..553
                     /locus_tag="LP524_002471"
     tRNA            464..553
                     /locus_tag="LP524_002471"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:500..502,aa:Ser,seq:tga)
     gene            566..639
                     /locus_tag="LP524_002472"
     tRNA            566..639
                     /locus_tag="LP524_002472"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:600..602,aa:Met,seq:cat)
     gene            658..730
                     /locus_tag="LP524_002473"
     tRNA            658..730
                     /locus_tag="LP524_002473"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:691..693,aa:Asp,seq:gtc)
     gene            734..806
                     /locus_tag="LP524_002474"
     tRNA            734..806
                     /locus_tag="LP524_002474"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:767..769,aa:Phe,seq:gaa)
     gene            811..881
                     /locus_tag="LP524_002475"
     tRNA            811..881
                     /locus_tag="LP524_002475"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:843..845,aa:Gly,seq:tcc)
     gene            894..967
                     /locus_tag="LP524_002476"
     tRNA            894..967
                     /locus_tag="LP524_002476"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:928..930,aa:Ile,seq:gat)
     gene            979..1050
                     /locus_tag="LP524_002477"
     tRNA            979..1050
                     /locus_tag="LP524_002477"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1012..1014,aa:Glu,seq:ttc)
     regulatory      1239..1318
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            1316..2509
                     /locus_tag="LP524_002478"
     CDS             1316..2509
                     /locus_tag="LP524_002478"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBL2235479.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            2639..4111
                     /locus_tag="LP524_002479"
     CDS             2639..4111
                     /locus_tag="LP524_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL2235480.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4194..4982
                     /locus_tag="LP524_002480"
     CDS             4194..4982
                     /locus_tag="LP524_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBL2235481.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5049..5717)
                     /locus_tag="LP524_002481"
     CDS             complement(5049..5717)
                     /locus_tag="LP524_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HBL2235482.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5832..7544
                     /locus_tag="LP524_002482"
     CDS             5832..7544
                     /locus_tag="LP524_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2235483.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            7547..9319
                     /locus_tag="LP524_002483"
     CDS             7547..9319
                     /locus_tag="LP524_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2235484.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2767 a         1643 c         2122 g         2856 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgccatgc gggtgtagtt tagtggtaaa accacagcct tccaagctgt
      121 tgtcgcgagt tcgattctcg tcacccgctt ttagtttttt atgggcctat agctcagctg
      181 gttagagcgc acgcctgata agcgtgaggt cgatggttcg agtccattta ggcccattag
      241 tacaaaatct tattggtttt ggggaagtac tcaagtggct gaagaggcgc ccctgctaag
      301 ggtgtaggtc gggaaaccgg cgcgagggtt caaatccctc cttctccgtt tcaatatttt
      361 gttttacctg gcccgttggt caagcggtta agacaccgcc ctttcacggc ggtaacacgg
      421 gttcgaatcc cgtacgggtc attaatttaa tatagtttta catggaggat tacccaagtc
      481 cggctgaagg gaacggtctt gaaaaccgtc aggcgggtaa aaccgtgcaa gggttcgaat
      541 cccttatcct cctttttcat attatcgcgg gatggagcag tctggtagct cgtcgggctc
      601 ataacccgaa ggtcgtaggt tcaaatcctg ctcccgcaat taaaaactaa aaacattggt
      661 tccgtggtgt aggggttaac atgcctgcct gtcacgcagg agatcgcggg ttcgattccc
      721 gtcggaaccg tttggctcgg tagctcagtt ggtagagcaa tggattgaag ctccatgtgt
      781 cggcagttcg attctgtccc gcgccattat gcgggtgtag tttagtggta aaaccacagc
      841 cttccaagct gttgtcgcga gttcgattct cgtcacccgc ttttagtttt tatgggccta
      901 tagctcagct ggttagagcg cacgcctgat aagcgtgagg tcgatggttc gagtccattt
      961 aggcccattt attttattgg cccgttggtc aagcggttaa gacaccgccc tttcacggcg
     1021 gtaacacggg ttcgaatccc gtacgggtca tggaagatac tttttgagta tctttttctt
     1081 tttatctgaa agaatgattt taaaaagctt agcagagtta tctttatgga tgattgtgct
     1141 aagctttttt ggacagttct aatgtaaaat atttttatat tttttaagca aaaaaatgaa
     1201 aaaatacgag cagaaaactg ttttatctgc tatacttggt tcgttgcaag ttcccgaaag
     1261 gatccgcagg acggagatgc cttgtaaccg aatttaacta gggggaatca ttataatggt
     1321 agaaagacac ttatttacat cagaatctgt ttctgaagga catccagata aaattgctga
     1381 ccaaatcagt gatgcaatct tggatgcaat tttaaaacaa gatccaacag cacgagtagc
     1441 gtgtgaaacg tctgtaacaa ctggtcttgt tttagtattt ggagaaatat ctacgacggc
     1501 atatgtagat attcaaaaag tcgtacggga aacaataaaa gaaattggtt atacacgtgc
     1561 aaaattcgga tttgatggag atacagcagc tgtattggtt gcgattgatg aacaatctcc
     1621 tgatattgct caaggagttg atgaagcact tgagatccgg gatgaagata aaaaagacgt
     1681 attagatgag attggtgctg gtgaccaagg tttaatgttt gggtttgctg tagatgaaac
     1741 accagaattg atgcctttac caatcgcttt gagtcatcgt ctggtacgac gcttggcaga
     1801 tttgcgtaaa tcaaatgaat taacgtattt acgtccagat gcaaaatctc aagtaacggt
     1861 tgaatatgat gatcaaggac aaccggaacg cgtagataca atcgttattt caacacagca
     1921 tgatgatgca gtagataatg aaacaattcg tcatgatgtc atcgaaaaag tagtgaagga
     1981 agttattcca gctgaattat tagatgatca aacgaaatat tatatcaatc cgactggccg
     2041 atttgtcatt ggtggtcctc aaggggatgc cggattaaca ggaagaaaaa tcattgttga
     2101 tacgtatggc ggttatgctc gtcatggtgg cggtgctttt tctggtaaag atgcgacaaa
     2161 agttgaccgt tctgccagct atgctgcacg ttatattgct aaaaacattg ttgcagcagg
     2221 gcttgctcgc aaagcagaag tacagctagc ttatgcgatt ggtgttgctc aacctgtttc
     2281 gatctcaatc aatactttcg gtactggaac ggttccggaa gaagaactga ttgctgcagt
     2341 gagagaaaac ttcgatcttc ggccagcagg aatcatcgag atgcttgatt tgcgtcgtcc
     2401 gatttacaaa caaacagctg cttacggtca ttttggtcgt acagatgtag atttgccttg
     2461 ggaacatacg gacaaagtag atgcgttgaa agcaagttta gcaaaataag ttatattcag
     2521 ctggaacgat cgccaagtgg tggttgttcc agcttttttt atggcagtta acctcttgaa
     2581 aaagatagta attatttatc tatttttaga tcctgtcata atgaaaggga ggaataagat
     2641 gaaaaaagaa acaaatgtta ctttggtcac agtgagtatt ttcattgcaa cgttcatgac
     2701 ggctattgaa ggtacaatcg taacaaccgc tatgccaacg attgtcggtt cattacatgg
     2761 aatggaaatc atgaactggg tattttcgat ttatttgctg acaaacgcta tgcttactcc
     2821 gatttacgga aaacttgcgg ataaagtggg acggaaacca atctttattt ttgggacatt
     2881 attgtttatt atcggttcgt tattttgcgg tttatctcaa agcatgattg ggttgattgc
     2941 tgctcgtgcg attcaaggga tgggtgctgg ggcaatgatg ccagtagctt tgacgatcat
     3001 cggcgatctt tattctattg aaaagcgagc aaaagtttta ggtttgaata gttcagcctg
     3061 ggggattgcc agtgtttttg gaccgcttgc gggaggattc atcgttgaaa cgatcagctg
     3121 gcactggatc ttttttatca atgtgccgat cggaattcta ttaattattt tgatttggct
     3181 atatttaaat gaaccaaaaa tcatacatga gtctaaacca atggatattt ttggtagttt
     3241 tagtttgatg gccatgcttg tctcattgct gttagggttc caattattag gagacgaagg
     3301 aatgagtccg cttgtttctg ctttgctgct aggtagtatc gtaatgttag gtatttttgt
     3361 ttttgccgaa aaaagagcta aagatcctgt catcatgatg catttattca aaaatccact
     3421 atttgttgtg gtcaacttgg ctgcagcatt ggctagtggt tttttgatgg gaatcgatgt
     3481 ttatatccct atgtggatgc aaggtgtatt agggctgaat gcagcaattg gtgggctggt
     3541 attagcacca ttatcactag tctggatggt cggttctttc cttgcaagca agtggatgac
     3601 tcaaatgaaa actcattggg tcttgagaat tggactacta atcgcagcta ttgggggcgc
     3661 ttggttgtat cttatgcctt tcagaagtaa ctatttatgg ttttttgcta tatcaacggt
     3721 catcggtata ggcttaggtg tgaccatgac tacctcgact gtcagtgcac aaaatagtgt
     3781 atctcgtgac cagcttggtg tagcaacttc ttttaatacg ttagttcgaa caattggtca
     3841 aacagtcatg gtttctattt ttggtttatt gataaatggg gtaactaaac gagaacttgc
     3901 agcagcaaat ttaacaaatg atgcagatat catgaataaa ctggtgaatc cacaaacggc
     3961 taaatatatt gatgcggata ttttgttacc attaagaaaa attctttatg atgggttaca
     4021 ttccgtttat ctagttggct tgttactcgt gatcattgct ttggtcttaa cgcttttcgt
     4081 tcatgaaaaa agtggtaaaa taaaacagta gtttcggtta agatatgtac gtagccgaat
     4141 cattcaaatt taaagttatt caaaaataac ttcttactaa ggaaggcgaa aatatggcat
     4201 ttattcaagc aaatatttat tcgaatgtgt tagaaatgga agtcaatcta aatgtgatac
     4261 taccgcaaac aacaaaaaag gtgattggca ccagttcaga gcaagagaca acggatatac
     4321 ctgttttata tttacttcat ggaatgggtg gcaatcatag tgtatgggaa agacgtacgt
     4381 cgattgaacg ttatgtagca aactacggat tagctgtcat catgccatct actgatcttg
     4441 gctggtacac agatacagtt tatgacatga agtattggac attcattgca gaagagctac
     4501 ctgagatttg tcatgaatta ttcccgcaat taacaagaaa aagagaaaaa acatatgccg
     4561 ctggattatc gatgggcgga tatggcgcgt taaaactagg ccttgcaaaa tcagaaaatt
     4621 tcgcagcagt aatctctctt tcaggcgctg tttctgtagg aaatcgaatg gatgatcttt
     4681 tgatggttcg taaagcatct ttttgggaag ggatctttgg accattggat caaatcgaag
     4741 gttcaaaaaa tgatccgcaa tacttattga aagaactagt aaaaagcgga aaaccagttc
     4801 caagatttta tatggcttgt ggggaatctg attttcttta tcaggcaaat caggaaatgg
     4861 tatctgctat gcaggaacaa ggcttggcgg tcacctttga acaaggacct ggcgatcata
     4921 actggatctt ctgggaccaa tggattcaac gtgcattggc gtggctaatg gaagaaaaat
     4981 aaaaaaagag tcttaaaaag atagctccta tagagatcac aaacatagtg atctctatag
     5041 gagctttttt actgctcgtt ttctcttctt ttattttgtt ggcataagcc ttcaatatag
     5101 gtttgtgcac tcgttagaaa gagtgctttt tcattcaatt ctgcatggga ctgcatactt
     5161 tctttaaatc cctctaattg attttcaact acataattac gcataaatag aactgtttct
     5221 ttcaaaaatt catttccgtc cataattttt tgtcgaagct gctgttcatg ctgctgatcc
     5281 attaactgtc caatcaataa attgtggaga gaggcaatgg cctgatgact ttgggtgact
     5341 gaaaatcctt cacgaacaag taattggatc atttgctcca aatgttctaa tcgactcgga
     5401 aaagcgggca cagtattgat ttctaattct gccccacaag ggaaacgtgt ataaaggtca
     5461 tagtaatttt ccatatactg cagcaactgt tcattccaag gcaaagttgg atctggaagc
     5521 gctaattctt cttccattgt ttcagccatt ttttgcaaga gctgttgttt gtttttgaaa
     5581 taccaatata aagcaggtgc ttgaacatga attctgtttg cgatgtttcg catggaaagc
     5641 tgcgaaagag cttgtgtttc agccaggata tcaaaggcgg cctgaatgat gattgcttga
     5701 gaaagcttta tttccatttt atcaacctcg tgtattgcat tatcttttta aacatggtaa
     5761 attgttagtc ggtctaattt tacagtgtta aattagtcct gtcaatttaa acaaaggggt
     5821 gtaatcaacg aatgaaatta atcttacgac atgcgaaaaa atataaagta gctgttttta
     5881 tttccttgct gtccgtagca gtcatggttg cagcagcatt atggcaacct aagttactac
     5941 agcaagtatt ggaagctatt attacagaag ataatgatga aatgaaaaca attggtattt
     6001 acctgatcag tctggctttg ctaggattag ccgcaggtgt aacgaacacg attttctctg
     6061 caaaagtagc tcaaggcgta agtgcagata tccgagaaga agcatttcga aagatccaga
     6121 cattttcatt tggaaatatc gaacagttct ctgctggaaa tcttgttgtt cgtctaacaa
     6181 atgatatcac gcaaatccaa aatttagtga tgatttcttt gcaatcactt tttcgaattc
     6241 cattcttatt tattggtgcc ttcattttag ctatgatcac gatgccgcaa ttatggtgga
     6301 tcatcgtagc gcttatcgtt acggtatttc tgattacggc actttcattt acaaggatgg
     6361 gcaagcactt catgatcatt caaaaactga ttgatcgtgt gaatagcatt gcaaaagaaa
     6421 acttactagg gattcgtgta gtgaaatctt ttgtacaaga agaaaaccaa ttgaagtcat
     6481 tcagcaaagt aagtgaggac ttaacaaaac acaacatcat agtcggtaca ttattttctg
     6541 tgatgatccc gtccttcatg ctggctgcca atttggctgt ggttggggcg atcttttttg
     6601 tgagcgatct tgttaaagat gatcctacgt taattggtgg aattgcttca ttcatgaact
     6661 acttgatgca gattatgatg gcaatcatta ttggcggaat gatgatgatg atgacttcaa
     6721 gagcagccgt atcgctgaag cggattgcgg aaattttaga tgctgaacca gatcttactt
     6781 atttagacgt tccagaacaa gagttgacgg gttctgtcac ttttgaccat gtttcttttc
     6841 gttatcctgg tgaggataca gatacgctta aagatatcag cttttctatc aaacctggag
     6901 aaatggttgg tatcgtcggg gcgactggtg ccgggaagtc aacactagca caattgatcc
     6961 ctcgtctatt tgatccgaca gaaggcaaag ttgaagtagg tggtgtagat cttcgtcaag
     7021 taaatgaaaa gagtttaaga caaacggttt cctttgtttt gcaaaaagca attttattct
     7081 caggaacgat tgctcaaaac ttgcgccaag ggaaaaagaa tgcaacagaa aaagagatgg
     7141 aacatgcttc ttctattgct caagcaaaag aattcatcga aaagttatct gatcgttacg
     7201 aagcaccaat agaagaacgt agcagtaact tttctggtgg tcaaaagcaa cgtttatcca
     7261 ttactcgtgg cgttatcggt aatccaaaga tcttgatcct agatgacagt acgagtgcac
     7321 tggatgctcg ctctgaaaag ctggtccgag aagcactcga tcgagatttg aagggaacaa
     7381 cgacgattgt catcgcacaa aaaattgctt ctgttgtcaa agcagaccgc atcttagtat
     7441 tggatgaagg cagattagtc ggcgaaggaa cccatgagga attggtagct ggaaaccgtg
     7501 tctatcagga aatctttgaa actcaaaaag gaacggagga gtaagtatga ctgatctaat
     7561 aaaagcaagt aaatttttct atcactattt gaagcggtac aaactctcct ttttctttat
     7621 ctttatcaca gtgatcattg ctacctatct acaagtaaag gcacctcaat acatcgggga
     7681 agcttttcaa gaattagcta attacgtagg aggcttgatg caaggcgtag acgataaaag
     7741 caaatttgtt tctgtaatat ggaaactctt gctgttttat gtactagcta gtgcagccaa
     7801 ctttatctat agtattttgt tcacacaagt agtagggaag tcaacgaacc gaatgcgtat
     7861 cggtctattc aacaaattgg aaaaattgac gatccgtttc tttgattctc atcaagacgg
     7921 tgaaatttta agtcgtttta ccagtgactt agataatatt caaaatagtt tgaaccaagc
     7981 tttgcttcaa gtcatcacga atgctgtttt acttgtcggg atcttgatca tgatgttccg
     8041 ccaaaatgta gagttagctt gggctacaat tgcttccact ccagtagccg ttttgatagc
     8101 tgtcttgatt atccgcaaag cacggaaata tgtggatctt cagcaagatg aagtggggaa
     8161 actaaatggt tatatggatg aaaaaatcag cggacaacgt gtcatcatca caaatggctt
     8221 acaagaagaa acgattgaag gattcttaga gcataatcaa agtgttcgcg aagcaacctt
     8281 caaaggacaa gcttattctg gtctactatt tccgatgatg caaggaatgt cactggtcaa
     8341 tacagcgata gttatctttt tcggcggctg gttagcatta aatggtgatt tggaacgttc
     8401 ggtggcatta ggcttagttg tgacattcgt ccagtattca caacaatatt accaaccact
     8461 gatgcagatt tcatctggat acagcatgat ccaattagcg attacagggg caagacgatt
     8521 gaatgaaata tttgatgaaa aagatgaagt caaaccagaa aacggtaaag catttgatgg
     8581 tgttgaaaaa ggtcttgcgt tgcagcacgt agattttggt tattctccag aaaaactagt
     8641 cttgaaagat gtctcgatca atgtggacaa aggagaaatg gttgctcttg ttggtccaac
     8701 aggatcgggg aaaacgacga tcatgaatct gctaaaccgt ttttacgatg tcaatgatgg
     8761 ttcagtagtc atcgatggaa tagatatccg agattatgat cttgatgcat tacgtagaca
     8821 tgtaggaatc gttttgcaag aatctgtatt gttctccgga acgatcaaag acaatatcac
     8881 attcggcaaa ccggacgctt cagatgaaga agtcatcaat gcagcaaaac aagcgaatat
     8941 tcatgacttc atcatgacgt tagaaaaagg ttatgataca gagatatccg aagaaaataa
     9001 cttattcagt acaggtcaaa agcagttgat cagtattgct cgaacgatca tcacaaatcc
     9061 atctttattg attttggatg aagcaacaag taatgtagac acggtaacag aagcaaggat
     9121 ccaaaaagca atggatgaag caatcaaagg aagaaccagc tttgtgattg ctcaccggct
     9181 gaaaacaatc ttgaatgcag atagaatcgt tgtcttgcgt gatggggaag tcattgaaga
     9241 aggcagtcat catgagttat tagcagaaaa tggattttat gcggaacttt atcataatca
     9301 atttgtattt gaaagttaaa ttaaaagaac ctcgttataa cggggttctt tttatatatt
     9361 ttatgttgtt tgtttttata aggggcag
//
LOCUS       DAFEOM010000091         9328 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.094, whole genome shotgun sequence.
ACCESSION   DAFEOM010000091
VERSION     DAFEOM010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.094"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            317..1102
                     /locus_tag="LP524_002484"
     CDS             317..1102
                     /locus_tag="LP524_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL2235485.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            1115..1966
                     /locus_tag="LP524_002485"
     CDS             1115..1966
                     /locus_tag="LP524_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HBL2235486.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(2090..2542)
                     /locus_tag="LP524_002486"
     CDS             complement(2090..2542)
                     /locus_tag="LP524_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2235487.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLMQTDISLVS"
     gene            complement(3007..3375)
                     /locus_tag="LP524_002487"
     CDS             complement(3007..3375)
                     /locus_tag="LP524_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HBL2235488.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            3525..4007
                     /locus_tag="LP524_002488"
     CDS             3525..4007
                     /locus_tag="LP524_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HBL2235489.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSEHMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLLNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            4044..5054
                     /locus_tag="LP524_002489"
     CDS             4044..5054
                     /locus_tag="LP524_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBL2235490.1"
                     /translation="MISYPLLERNTTIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGINLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(5169..5882)
                     /locus_tag="LP524_002490"
     CDS             complement(5169..5882)
                     /locus_tag="LP524_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235491.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSSDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPTHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
     gene            complement(6072..6662)
                     /locus_tag="LP524_002491"
     CDS             complement(6072..6662)
                     /locus_tag="LP524_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBL2235492.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            7185..7856
                     /locus_tag="LP524_002492"
     CDS             7185..7856
                     /locus_tag="LP524_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBL2235493.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLAENYDNHVVIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGY
                     SFILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEM
                     FINAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPNTIYFKRV
                     GGGIT"
     gene            7853..8014
                     /locus_tag="LP524_002493"
     CDS             7853..8014
                     /locus_tag="LP524_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235494.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMKYVADEANRK
                     QRKLVGLDK"
     gene            8498..9268
                     /locus_tag="LP524_002494"
     CDS             8498..9268
                     /locus_tag="LP524_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HBL2235495.1"
                     /translation="MNPTLITKKELLKKLDISTGVLADLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCFKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYL
                     AGQIYTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDIYNQEENQTRHIRNLSDAKLE
                     EEARKVSNYNKKMAAKYVNEEEFCFSKTKIYKRTRGAS"
BASE COUNT         3209 a         1445 c         1763 g         2911 t
ORIGIN      
        1 ggctgtgaca aaagtcttgt cacagccttt ttttccaaat aaatggtttt tcagaagtag
       61 cttcttcgga aataagccga aatttcacaa aaatttgaga agtaattttt gtaaattact
      121 tcttatttct tgaagctggc cacttctgtc acaacctctt tctttataga aataaaagga
      181 cactagcaat tcatgaaaac gattgctaga gcgtatcatt tgctttagac tatatatctt
      241 ggtgtacact cactttaaga cggaaataaa agttatccgt aataacaggt ttaggtatga
      301 aaaaggagtg ggtactatgt ataaggaatt agaaaacaaa gtcgcagtag taactggtgg
      361 ctcaaagggg attggaacag cgattgctca acgattcggg aaagaaaaaa tgaatgtagt
      421 tgtcaactat aactcggata aagaaggagc agaaaaagca gtcaaagcaa tcgaagaggc
      481 aggcggaaaa gcagtctctg tacaagcaga tgtcagttct gaagaaggaa tacagcgttt
      541 acttgatacg gctttagaaa attttgggac actggacgtt tggattaata atgctgggat
      601 ggaaaatcag catccaaccc atgaacttag ccttgaagat tgggaaagag tattaaaagt
      661 caatttgact ggcgttttta tcggaacgaa agcggcttta aattacttta cagagaatga
      721 taaaaaagga aatatcatca atatgtcttc tgttcatgaa caaatcccat ggccaacgtt
      781 tgctcattac gctgcttcaa aaggcggagt aaaactattc accgaaacgg tagcaatgga
      841 gtacgcacca aagggaatca gaatcaatag tataggacct ggtgctatcc gtacacctat
      901 taatgcggca aaatttgatg atcctgaaca aaaagaactc ttggaaagta tgatcccact
      961 aggacgcgta ggtgaaccaa aagaaatagc ggcgacagct gcatggttgg cttcagacga
     1021 atccagttat gttacaggta ttacactatt tgcagatggt gggatgactt tatatccttc
     1081 tttccaaggt ggaaaaggat aagggtgaat aaaaatgggt ttattgattg cattgatccc
     1141 ggcagttggg tgggggatcc agccactgat tttgaaaaag attggtggca gaccaacaaa
     1201 tgagatatta ggaactggta ttggagccgt gctcgttggc ttacttgttc aattattcgc
     1261 ttctccagct ggtatttcag tgactacatt tttgattagt ttactttctg gtgcattctg
     1321 ggtcatcgga caaacaggac agtatacatc tttcaacttg attggtgttt ctaaaacgat
     1381 gccaatttcg actgcattac agttagtcgg aacctcattg attggtgtct tcgcttttgg
     1441 agaatggtct ggaagtatgg gaaaaatcat tggtgcagca gcaatcattc ttcttgtatt
     1501 tggttctgct ttaacggcaa tcagtgatga tcctggaaaa aaaggcggta tcacaaaagg
     1561 aatcagtcta ttagcatcaa caagtattgg ttattgggtt tacagtgcat tgcccaaact
     1621 agttgatgca agcggacttt ccattttctt tccgcaaatg atcggtgtct ttttaggggc
     1681 agtgatttat gtgattttcc gaaaaccaac cgcattttct gaagacaaga gttggaaagc
     1741 aacaatagtt ggagccatct tcagtgtgtc agcattggct tatatttttt cagcaaatga
     1801 aaatggggtt gctactgcat atatcattac acagctaaac gtagttatct ctacattagg
     1861 cggtatgtac atcctgcatg aaaataagac accaaaagaa aaaaaattaa cgattatcgg
     1921 attattgctg atcgtagtag gaagcatggt cacggtcttt atttgaggat aaagatggta
     1981 aaatctcatt tattaaaaaa atatttgttt gaaccaaaca gtaagtactt ataaaaaaat
     2041 agcgctagaa tttccaacat ctctggaatt tctagcgctt tttaattgtt taagatacaa
     2101 gtgaaatatc tgtctgcatc aataattttt cattgatcca ttgactaaag tttacgacat
     2161 aattgaagtt accaccgatt agtatctccg catgattgtc tttataaaga tcgtattgta
     2221 aaagacggtc ttttttattt gcgtaaacat cgttatggta ctggactaat tgttctcgga
     2281 attgattgat tgctgtaaaa tcacgatact gaaggttgaa acgcatcgtg attggtgcat
     2341 ttagcatgaa tcgctgcatt tcttttgctg cttcctctgc ttgatctccg tataggaact
     2401 caaaaagatt acgttcagaa aatttccatt gcccgccaac ctttgttcct tttagttttc
     2461 cttcatgaag ataatttctg atagaacgtg tagtaacacc taattgttcg gctaattcat
     2521 ttactgagta aaaatctttc aatgttcacc ctcctatgtg aatggttcta ctgacttagt
     2581 tgatatttgc ttaatcacga aatgtagaaa gaacgatttt aattaaggtc attgtaaaaa
     2641 tcaagtcaga aaattctaac ttttctttct ctaaagaaat tcgttctctc ctaaagtgat
     2701 attatattta aatatacctg aaaataatgg gcagtattat gaaagatcac tgatattttc
     2761 tcgtattttt tccttattca aaagttttgc aggaaaattg ctgaaaaaat aattttacta
     2821 agtggaaaat tcaaagccat tcaatcagtg atttttttca gcacaagaaa gactccttat
     2881 aaaaatagca gatactttgt caattaagtg tttaaccagg gatgataaaa aggataggat
     2941 tgaacaaaac ctgtataagc tgatgaatag tttcctcgcc agtaaaaggt ttattcgtat
     3001 aaagctttat cgccaaaatt tatcatgacc ttttttatct tcaatattaa atgaaatcaa
     3061 ttgttcaatc aattcccagt tgattacact tttccaagga actttgatga cgttatctgt
     3121 atatacgtag cctgcttctt ggatattttt ttcaagggca cgaatcgctg ctttttccgg
     3181 tgcgatagaa aaatgatttt tagcaaaact aaatgcaata ataaaggtac catggtcagt
     3241 aaacataggt tggttccatt ttacagttgt gtctagctgg ggatacgttt ctgttatctt
     3301 agcaaaaagg tctttgatct tttgttgatg ttctttttct ttgatctgat tgatgatcgg
     3361 ctgaaatttt tccattctaa aacgcctcca ttaagtggtt tgatttattt taccagaaaa
     3421 acaatagtta tgtataaaaa acgaacaaaa cacaagtaat gttgtaaccg ctatatttat
     3481 atgataagat gtctcaaata ataaaataga aaagagacat aaaaatgatt gacttggcaa
     3541 agaaattctt acacgaaaat atatattttg agttgtatca tcatcgagcc atttatacaa
     3601 atgaagatgc tttggtaata aagaaagaac aaggatttac aggtacagag accaaaagct
     3661 tattcttaaa agataaaaaa gataataact acgtatatct gacttttact acgaaacaaa
     3721 cggatttcaa agcattgggg aagatcgtcg gtaaaaggct ttctattgtt tcatcagaac
     3781 acatggaaga acaaacggga caaaagcagg gcgctgtttc tccgtttggt tatgaaaaac
     3841 agattccgat tattattgat gaagagctct tattaaatga aaaacttgtt tttgcaccag
     3901 gtagaccaga tcaaacgatg gtcatccaaa cggttgactt aaacaaaatc atccagctac
     3961 ttgagatgcc gatatttttg atgccggcaa acgaaaaaaa caattaacca agtaaaatag
     4021 tgcatacttg gaggtgaagc aacatgataa gttatccatt attagaaaga aatacaacga
     4081 ttggggttac tgcaccatcc tcaggcgtat cagaggaaca ccattcgtta ctcgaatcag
     4141 ctttaaagag aatggaagaa aaatctttcc aagtagttac tggaaagacg ctttggacgc
     4201 aagataagga aaaatcaggt ccagcaagaa aacgagcaga tgaattcaat caaatgatgc
     4261 aagatgaaaa tatccaaatg atctttccgc cttggggagg agaattattg attgagattc
     4321 tcgattttgt cgattttgat gctctacaac caaaatggat agtaggttat tcagatatta
     4381 gtacgttgct acttgctgtc acattaaaaa cagggatagc cactgcacat ggaatcaacc
     4441 ttattgattt aagaggagag tacttggatg aaacgacagc aatgtgggaa agtgtactga
     4501 aaacaagaga aggagaatcg attctacagc aaccttctga aaagtatcaa aaagagtggc
     4561 cccatgagca ttcatcatct tctgttttcc atctcactga accaacaaat tggcaatcag
     4621 tcagcggaga tgcagtaagg atgaagggac gattattagg tggctgtatt gatacgatca
     4681 gtcatttaat cggtacccct tatggagatg tccaatcttt taaaagaaag tataatgatg
     4741 gaaagccgat cttgtggttt ctagaaaatt gtgagctatc gtcagttgat ttaagaagat
     4801 cacttgttca gatgaaactg gcaggatggt ttgatggcag ctcggggatc atgttcggta
     4861 gaagtgcagc agaggatttg ttagattata aaatgaagga tgtatacgaa gatctgtcaa
     4921 aggaattaaa actaccgatc ttgtatgata ttgattgtgg tcatgtaccg ccacaactga
     4981 cattgatcaa tggtgcctat gcagaagtag cggtagaaga tagaaaagcg actgttttgc
     5041 aaacgtttat ttaaaaaatt ttttcctgcc ttggagttta tcaaagacca acaaatctaa
     5101 atagaaaaga aaatgaaaga agctgacagt attttgccag cttctttttg tatgatctat
     5161 tagaaatttt aattatccat aactatcaca ttgatcagct gtttcacatc atttggatta
     5221 cctgtaggaa tagctactac agatgcatta tcaataattt cttgagttgt ttctctaatg
     5281 aacgctggat aagatgtttg atctgcaggc atttgccaat gtgtaggaac tggataacta
     5341 gtgattgtcc cgtcatggtt acttgaatac actaaaactc cctggtaact gtatttccca
     5401 gtgagcataa ccacatcttt cggatagact gcactccctg catcataagg ggcgataggt
     5461 gttcctgcag gataatgaat aacatgtagt tcgaaatcac tcgatgtatc tctaaaagta
     5521 ttacctctaa tagctagcca aacacgtgcg tactcaattt gtagatcaga atatcctgct
     5581 aacggatcta ttgactgaga agactgtgag gaagttgtgt ctattgttga agaagaaata
     5641 tttgtctcaa ctgtactact agatgtagaa gaagtattgg gctctgagac tttactgctt
     5701 gttgaagatg tattcgtttc agtagaagct gatttctgag tacttgattt ttcagctgaa
     5761 ctggtttgtc tgattgaact tttgtctgtg gtcgcagatg gatcactatt ttctttacta
     5821 ggagacgtac atcctacaag gaataaagag accaacaata aaataaaacc taactttttc
     5881 atataaaata cctcattatt tttttaatac cagtatagcg taatcctgaa tttttgaatc
     5941 cgtaaaacct tgtctatgaa tataaataga gtatatcttc tttgataaat gtcagaaaaa
     6001 gtgtgacaaa taaccatcag taaaattact tgtcatacta ttgagaagga gtatttaatt
     6061 gaagagaatg attaggagaa aggatctccg tactttttat agtaatcata gaatccaagt
     6121 ttgtatagac gaatcgtttt ttctcttatt tcttctgttt caaaatggac tgcttgaaga
     6181 atcaagtctg tgaattctat aggagctgtt ccattcgcag taatcaaatt acgatctttc
     6241 acagcttgtt gttctataaa atcttcttta ttctgataat tttccgattc taaccaagga
     6301 aattgcgcat ttccggtgtg tttgtaacct tgcaataatc cgctctgagc taaataatca
     6361 actgccccac aaatagcagc tactggtacg tctgttttta acaatttatc gattatttga
     6421 tataaatgat gattttttat gtcccatgaa ttgccaccga tcataataaa aaggtcaact
     6481 ttttcaggta tttgttcgat taagtaatcg atttttgttt ttaatcctcc aatggaagtg
     6541 acctctgatt ggatagaagc agttttgttt tgccatccct ctttttggtt tagtgtgcta
     6601 gtaatataag caccttccca atcggcatat tcctctaata gaaacgtaat cgcagttttc
     6661 attttcaagc tctcctttac tatctatgac aaggacaaca gttttaacat acagacattc
     6721 attaaatttg gatagcgaca gcgaaaacaa caattcttaa gttttagaca gagtatacag
     6781 acagtcaact taattaaata cgaactaaat aaaaatgaca ggtaatcatt tttgtttacc
     6841 agcattataa cattttgcat acatggatga caaaagaacc aattcccact tgcttgagta
     6901 caatcttaaa agaaactgga aggcaaaaag taattataga aacgaaaaat aaatcatatc
     6961 gggcactcag acatgaaaac aaccctagaa atatataact agtccaatga aaaaacagcg
     7021 aatgccgtag aaaacaagga ttctgcgtac attcatatct cattttgcga gcatatctgc
     7081 tattactgtg actaaatgtt aatatggtta aaatgaccgt tatatttttt atataaatat
     7141 cgtataatta gtatgaatat agaagaagga aagaaggtgc aatcttggaa cctaaagaca
     7201 agtatttaga ttacgctagg gtcaataaag ataaatttat tgaaacaatc atcaagggaa
     7261 aaatccctga tgatgaaaaa gatgcggtat tcatggctgg aagcccagga gcaggtaaaa
     7321 cagaggttgc actaggttta gctgaaaatt atgacaacca tgttgtgatt gatgcggact
     7381 attttagagc acaatttcca gaatacaatg gaaagaactc aagtgttttt cagaaagctt
     7441 cttcttggtt agttgaacaa tcattaaaat atgtactgga acatggctat tctttcatac
     7501 tagaggatac ttttgctatc cttagtgcag agaaaaatat tattcgagcc cttaaaaata
     7561 actttcgcgt tacgattttc tatgtttacc aagatcccaa agttgcctgg gatttcactc
     7621 gaaaacgtga actagctgaa ggacgtcatg tgccaaaaga aatgtttatc aatgcgtttt
     7681 ttaagtccag agagaatatt gaaaaagtca aaaatagaca tcctgaagta gtccttcaca
     7741 taatgattaa ggattatcaa aacgacattt ctgaagtgca ttatgcagca gataatattc
     7801 aattagtact acccaataca atatacttca aaagagttgg aggaggaatt acatgactga
     7861 gaaagaacaa gtacaacaaa tcgtgaagaa gtataataaa agtatcgctg atttatcaga
     7921 aaacgcctca gcgaaagaat ttaaaacagt aatgaagtac gtagcagatg aagcaaatcg
     7981 taagcagcga aaactagttg ggttggataa ataaattttt taaaccatat ccttgtaaag
     8041 ttgagttttt tgttttaatg aattaagact attaaataga taattattgt aacttaaaaa
     8101 acacagctaa aaattagttg tgttttttat taggtttact tttctggtat aaaaggaact
     8161 agctactttt tcaaaatttc taaatcattc aatatgtagc gcttactaaa ttattcgcca
     8221 taaaatttta taattggatc actcattgtt ttacctaagt catatttaag taataaccgg
     8281 tgtttttaca taagaagact tggggtattt tttagtttga ttagctctga tatacctata
     8341 aagaatatgc taaggtaaga atagtaaaaa ttcacataga tgaggtataa taatgatgtt
     8401 tttatatatt acagataact tgacacaaaa tgagtttttt tcgatttttc aagttatcta
     8461 atgcaattat attgatatca ttaggagctg cattgttatg aatccaacat tgattactaa
     8521 gaaagaatta ctaaaaaaat tagatatttc tactggtgta ttagctgatt taattagaaa
     8581 tggcatgcca aaagagggag aaatgtttaa tcttgataag attattacat ggcgagaaaa
     8641 ttggtctaaa aacatattag gtgagttaga agttggtaga gtatatacta acaaagaaat
     8701 ttcagagaag tttaaatgtt tcaaacaggg tggaatgaga agatctcatc aaactaatac
     8761 tcttgtactg tttagtgatc aaacaggaag taatgtttat aaagataagt ggttaaatgg
     8821 tattttacaa tataccggta tggggctaaa aggggatcaa gtattggata agaatcaaaa
     8881 taaagtttta gccaattcaa aaagtaattt tgtaaagatt catttatttg aaacatttaa
     8941 accaaaagaa cacacgtatc tgggagaagt atatttagca ggacaaatat atactgttaa
     9001 tgaaaaagat agttcgggca acagcagaaa agtatataaa tttccattag ctttaataaa
     9061 tcaagagcaa ttgattgaag ataaagatat atataaccag gaagaaaatc aaactagaca
     9121 tattcgtaat ttatctgatg ctaaattaga ggaagaagca agaaaagtta gtaattataa
     9181 taaaaaaatg gctgcaaaat atgttaacga agaggagttt tgcttctcaa aaacaaaaat
     9241 atataaacgt acccgtggtg ctagttaatt ttaatatcct aaagaatatc tcaaagttcc
     9301 ttcaaaacgt cgttgtgctc gacttaat
//
LOCUS       DAFEOM010000092         9209 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.095, whole genome shotgun sequence.
ACCESSION   DAFEOM010000092
VERSION     DAFEOM010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.095"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            470..2107
                     /locus_tag="LP524_002495"
     CDS             470..2107
                     /locus_tag="LP524_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235496.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(2252..2992)
                     /gene="truA"
                     /locus_tag="LP524_002496"
     CDS             complement(2252..2992)
                     /gene="truA"
                     /locus_tag="LP524_002496"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBL2235497.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3183..3818)
                     /locus_tag="LP524_002497"
     CDS             complement(3183..3818)
                     /locus_tag="LP524_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HBL2235498.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3965..5206)
                     /gene="sstT"
                     /locus_tag="LP524_002498"
     CDS             complement(3965..5206)
                     /gene="sstT"
                     /locus_tag="LP524_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBL2235499.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            5404..6400
                     /locus_tag="LP524_002499"
                     /pseudo
     CDS             5404..6400
                     /locus_tag="LP524_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
     gene            6416..8434
                     /locus_tag="LP524_002500"
     CDS             6416..8434
                     /locus_tag="LP524_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HBL2235500.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8494..9186)
                     /locus_tag="LP524_002501"
     CDS             complement(8494..9186)
                     /locus_tag="LP524_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HBL2235501.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATVATTTTTSTESKSTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADKSAQLEAF
                     MTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWSESGTGQA
                     DYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELK
                     NGFNQIVYGQAN"
BASE COUNT         2978 a         1706 c         1445 g         3080 t
ORIGIN      
        1 actaatcaga atcgtacaaa tatttaataa ccaaatatat agaacataac ttaatattaa
       61 gttatttgaa attccataaa aaacgactgc aggaattatt gtaagaaaag gataaaaata
      121 gttcagtcca tctccataat tgttaaatgt tgtatagttg attggccctt caaaaataga
      181 agataaccct ttgattcgtg aaagatgaaa agataaatca cctgttgtat aaatttcttg
      241 agcatttctc ataaaattta ctgaatataa agtactaagt aagataagaa ctactaaaag
      301 aaatgctttt ttacttattt tcatagattt gttgttctcc ttttttaatt ctattttcag
      361 tatactagaa ttgtcttttt ttaataagtt ttgcgttaaa tttttcatag cactagtatg
      421 atatactaga aaagaatcaa tttttattac ttttaaatag gagaaaaaaa tgaaaaaaaa
      481 caaaaaccaa ataatagata tcatttttat gttatttcta agttttgctt acgtcatccc
      541 tctattcatg tctatgggaa tctatcatag tgtcaatcag gatacttact ttcatttatc
      601 tcgaatcata ggactagata atgtatggag ttctccagta aacttcaata acttcgatca
      661 tcatggaaca atgatgaata ttttttatcc atggcttact ctttatccag cttttttatt
      721 ttataaaatg atgggaaatc ttgttttaag ttataatatc tactattttt ttatcacttt
      781 tttaacaatg gtcgtttctt atttttctat gaaacaaata aaaaacaatc gttatatttc
      841 gctgttattt tcgattattt atacattttc cgcctatcgt gccattgaca tattccatcg
      901 tgcttcattg ggagaagctg tcgctttaac atttttgcct ttgatactaa tggggtgtta
      961 cgaaatttat attagagatt accaaaaatg gtactggttg tctattggca tgacgctagt
     1021 agtgtatact catttacttt ctgtcgctat ggtttctgta tttattggag gaacgttgtt
     1081 tctttctttt tatttttggg accaaaaaat cgctagactc ttaagtcttt taaaagctac
     1141 cgtcctcacc ttttttttaa gtgcgggatt cttaattcct tttattcaac aatctagagc
     1201 acaagaattg aaagtcccac tgggaaaaga attaagtggt atggctccta gtgatatgct
     1261 gacacacatt ttaaataata actataacaa ctatactatt ggattattct tatttctcgg
     1321 attgattggt gcttttattt ttataaaaaa attaactgct gatgatttat ttattttctt
     1381 tcttggtgta tttgttcttt tttgttcaac caatttattt ccttggcaac tttttaacca
     1441 tacaccagtt aagagtctac aatttgtctg gcggttaaat ggtttttctt ctttatttat
     1501 agcctatacg atgagtatag tgatctatta tattttttct actaaaaata attcatggaa
     1561 aataatggtc tttacgtttc ttgcactaat attgcatctc tcaggagtaa gcaattctat
     1621 ccatgctaac aaagattcat taacacttat agcacctgaa gacgctgttg ctatagctga
     1681 aaattacaat catactgatt atgcaaacaa agagtcaatc tatcatcctg atatgattaa
     1741 taatgatcaa tatttactag gtaaccagga aataaatcca acaactcatt ttatgagtaa
     1801 caaacttaca attgaattag ataattcgaa taataaaaat actgtcttga cgacacctat
     1861 ttatcgttat aaagggcaag tggcttctat taatggcaaa ctagtacaaa caaaattatc
     1921 taagtttggc acaactgaat taactattcc tccaggtata aataaagtcg ttattactta
     1981 tcagtacaca aaactagcaa ttgcttcaag atatttatct atcgttactt tgattttatt
     2041 tttactttat cgatttactt tttctaaaca aaaacaaccg agaagagaag tacaacattc
     2101 tcactagatt tttatttaag aaagactttt tagctgtaca cataaacagg ctaaaaagtc
     2161 tttttttata tttctttaat ttgcttcata cattatttaa catcgatatt tatactgtga
     2221 aacatgcgtt tcatccatta tcaaagtatt tttatcgata ataaacttca tccaaaaaca
     2281 gaccttgtgc agggacggtt tctcctgcat gttgccgcac tttgctttca aatatttcat
     2341 cgattgtttc tatcggcatg gcacctgtgc cgatttctag taatgtcccc atgataatcc
     2401 gcaccatttt atataagaat ccgtctccga caaaagtaaa atgaagcatt tctccctttc
     2461 gttcgataga taattcatca atcgtacgca ctgtggattt ttttgatttt ttcaatgcag
     2521 aaaaaccaat gaaatcatgt gtacccacta atttttcgca agctgcattc agcttgtcca
     2581 tatccaactc ttgagggaaa taaaagctgt ggtttcgctc aaatgctgaa ggaatcacgt
     2641 tgttccaaac ataatagctg tattttttgc ccaccgcatt atatcttgaa tggaagcgct
     2701 caggcatttc ttcgattctt ttgatgacaa tatcccgcgg aaggtaacga ttcataaagt
     2761 cgatcatttc tgccagtcca atttctgtgt ttgttttaaa atttgctact tgtcctcgcg
     2821 catgggtgcc agcatctgtt cttcctgatc caataatttc tatctcttca tttgtcatct
     2881 gatgcaatac tttttcgatt tttccttgaa tggttttatc gtcatctcca agtctttgcc
     2941 atcctgaata tctttttccg tcatattcga ttgtcaactt gatgtttctc atattttagt
     3001 ggctcctctg tcttccattt ggtcttctct ctaccttttt aagattgtag cacaaaatat
     3061 agctcctttc ttctctaaaa cagaagctat acaagaaaac tgtggaaata tatatttcag
     3121 aaaaacaaaa aacagccacc ggatgaagta tccgctgact gttcaatttg acggctcaag
     3181 ttttactgtt tgagatgcaa gatactcaac caggcaatgg gcacataatt aaagaaaaaa
     3241 ctgattcttt ggcaaaaacc tattttattc agtaatggcc aagaatttat agaaggaatt
     3301 cttgccagtc cataaatcgc cgcaaaaagc aaactgatca cagacagtcc aaggtataag
     3361 tggcttacgt ccatttgccc attttttcga tagatcaaga aaagaagaac tggaaacaat
     3421 aaaaaaccac tgtaacctaa acctgatccg atattatgga cccatgtcga gacattccac
     3481 gaagattcat gggtattgat actgaacaaa cctgtaaaga tacaatcacc tataccatag
     3541 aacgcgatgg ctgctgtaac tagggtagct aacacacgag atgtacttac aaatgcagca
     3601 tacaatgcag gcaatgacag gatgaaaaag atccctgaaa ccacagacca aataagaaac
     3661 gcttttctta caggactgga aacctctcca aatacactga tcacgctctt catttgatct
     3721 aattcaggat aatacaatcc taatacgtac ggagtcaaaa aatcactcag cacgcctaat
     3781 aataaaaaga aaaaaccaaa tttacgtaaa aaatccatga tacgcctcca ataacaatta
     3841 cctatgcttt taattgttat tataaacgac tttatcttga tttaacagaa acctctattt
     3901 acacgaacat ctatataact cattaaaaac tcgaatcatt ttaaaaatac taaatatcta
     3961 acgttcaacg ttcacttaat ccatctaaac aatctttcaa tttgattttt tccccacttc
     4021 ttttacgcgc gcctagctct cctattgcag taaacaacag atcacttgac gaatttaagg
     4081 cagtttccat tgaatcctgg ataacaccga taataaatcc aataccgacg atttgcatcg
     4141 cgacatcatt agagatacca aacaaactac atgctaatgg aatcaataac agtgaaccgc
     4201 ccgcaacacc tgaagctcca caagcagcta aagcagacaa aatacataaa aggactttca
     4261 aaacaaaagg tgcttcaatc cctaaagaat gtacagcagt caacgtcatc aaggaaatgg
     4321 taatcgcggc accgcccata ttgatcgtag cacctaacgg gatcgatatg gaataagact
     4381 cttttgtcaa tttcaatctt tcagccaaca tcatgtttat cgggatattt gcggcagaac
     4441 ttcgggtaaa gaaagcggga agtgcgcttt ctttcaaaca aaagaaaatc aatggatagg
     4501 gattttcttt aataaacaaa aacgtgagga atgggtacac aactaatgcg acaaacagca
     4561 tcgtaccgat caaaacaagt aaaagctgca gatactctgc caatccagcg attccagtcg
     4621 tagcaattga ttgataaacc aatcctaaaa tccctatcgg agcaaatgca ataatcactt
     4681 gtacgacttg tgacaacgct atagatatct gatccaccaa ctcttttgtc gattctgaac
     4741 gtgctcgaaa agctagtccc aacaatacag cccagaataa gacgccaagg taattcgcat
     4801 caataagaga ttgaacagga ttttgtgtga cattcgtcaa catagtagat aaaacacttt
     4861 ccaaactttg cggtgctttt tcagatactg tttcttgtaa agtaatagga acagtaaaaa
     4921 ttgaacttac aataactgct acaagtgcag aaagcaatgt tcccactaaa tataagatca
     4981 aaataggctt cacgaatgtc ttattcccaa ttttatgctt ggcaattgac gccatcgtca
     5041 agaaaaaaac cagaataggt gcgattgcct tcaagcaact gacaaacaac gttcccaata
     5101 ttgaaataaa tgtccatgtg gggaaaaaga taccaaacaa tattccgact aaaataccaa
     5161 caacaatacg ttgaactaac gacatatcca aaaacttctc taacaaatga ccaccctttc
     5221 acaattttaa caatcattgt atcattttat gcaaagaaac taaaggtata attttctaaa
     5281 aattctaaca atacaaacaa ccatttcttg ctaattttgt actttttctg tctacacgtt
     5341 gttttttgag atattctaag ttaggatgtt attaatgggc aacttatcag gaaacgtttt
     5401 cttatgtttt tgtccaattt ttttgtgagg tgtttattct tgaaaaaaat aaagaaggag
     5461 agttatggct tcctgacaat ctttgtcgct tgtataatga tggttttaca gcgtatattg
     5521 atttcagcag tcactattcc tacatatgca acaaaccctg gtcgattcta catctataca
     5581 tttttacaaa cttccgttgt tattggtgca aatttgatcc cactttacat gggttaccaa
     5641 tatcaaaaaa tcgcccgttt aaaagtttta cattatttaa gtcgttttgc ctttatattc
     5701 ctaatagcca cactagtttg caatatcttt tttttacgta caagctggca gcttgaatat
     5761 tcgtgattat tggcttatcc taacacctat cagtcagaat tactttactt atgctgtttc
     5821 ttgtactttg cttttttgta gtattcctta tacagttgga tttttaaata aattatcgaa
     5881 aacaacaatt aaatatctac tattattttt aacaattgga ttagttggct gctctactct
     5941 atttaataaa gacccgtttg cattacagaa tggacagagt attttatgga ttttttattt
     6001 atttttaatg ggttatggtt tgaaagaatg gaactggaaa gaaaaggtcc gctataagct
     6061 tcctcaagta tttatcagct tgattgtttt attcggtttg attattttga tgacacaaat
     6121 ttcactcatc atcagaggaa atacagatac agcgctgagg ttttctaaac catattctct
     6181 cttcgcaatg tattatactt tctcttcgtt tttattttta gaaatgctat ctgataaatt
     6241 aggtataaaa ggaagaggaa attttcttgc tgtatttttg attgtcactc aagttatatg
     6301 taatttccct ttagtttctt atcgcgtatc tacatttatg aaacgtgaat ttccaaactc
     6361 agggaaggca tggctgataa atatcgggca gttttttatt tgctatttat tagcagtgtc
     6421 cctcctagtt atctttttat tttatataca aaaaatccgt ttcattcatc gtttcatcga
     6481 atacttcgct tttgattcga ttgaacaatt aactcagcgt tggaaaaaaa gaattcattg
     6541 gctttttgtt cataaaagtt attttttagt tgcagcattt ttttactgct ttaccttcgt
     6601 gcagattttc gttttagaac caaaagaagg ttggaaagaa actatccagg ttgccttaaa
     6661 aatatttact cagcgacaag cacccatgat cctgacaatc atcattatta tgggattttt
     6721 cttcttacta ttgttgattt ccaatcgttt ttggtacgcc ttgactgcta ccttgatcat
     6781 taacttactg ctcactattt cgacagtaat caaaatggaa atgcgggaag agcctgtctt
     6841 tccttcagat ttaaaaatgc tgaccggatt gtcagaacta ttatcgatgg ttagcccagt
     6901 cttattaatc gtgggcggat tgatccttct cctattattg attactagta ttattgtaca
     6961 acgtagacta caaaaacagt attccttgaa gattcattgg aaacgtcgat ttatcagtat
     7021 cgctgtatta ttaggatgtt tttcgggtgt ctttttcatt aatcacaaaa attcgccttc
     7081 tttcttgctg tttaatttat ttaaagtaaa caagactttc tttaaccagc aaggcgccgt
     7141 aaaagataac ggaccagtca ttcaattcct aaacaatatc gacattaaaa tcatggaaga
     7201 acctgctggc tattcgaaag aaaaaattaa tggaataatg aaaaagtaca acacaaaggc
     7261 agatgaaatc aaccaaacaa gaagtgactg gttaaacaat cagacgatcg ttctcaattt
     7321 aagtgaaagc ttcagtgatc ctgcacgtgt ccctaatctg acagtaccta cagaccctat
     7381 tccttacatc aaaagcatta taacagaagg aacaggagga ttgatgttat ccgtcggtta
     7441 tggtggcgga acagctaaca tggaatggga aggactgaca ggcttgagta tcagtaacct
     7501 gtctgcttcg ttagttacac cttataccca gctagtggag cgtcagaaaa taagtccaaa
     7561 tattaccgat ctattcgatg aaaaaatagc catccatcct tttacagctg ctctttatcg
     7621 cagaaaacaa gtgtttgata aatttggttt tgacaggttc tattatttag acagtccgga
     7681 taaattaact tatacggata aaatagaaca aagcccacgc atatcggatg attcagcata
     7741 caaagaaacc ttgaaagctt tacaaagcaa tacagaaacg tcacaattca tccaattatc
     7801 cactatgcaa aaccatatgc cttatgatga ttactatagt gaattgaact acactgctga
     7861 aggaactgct gtcattccta atagaagaaa agaattacaa acttatatgc aaggccttca
     7921 ctataccgat acagcagtaa aaaaattcat ccaagaaatc gacaaaatcg ataaaccgat
     7981 tacctttgtt ttttatggtg accatttacc cgcaatctat agtgggaatg attcgaaaaa
     8041 atatggatta gaacagcacc agacagatta tttcatttat aacaacaagt atagtcgtga
     8101 acaagcagct acgttaaata aagaagttgt tgctcctaat aactttggtg ctatggctct
     8161 tgagcatgcg aatatcaaag taactcctta ctatgcgttg ttgacagaag tatctaatca
     8221 tctacctgca ttaacaattg atcctacaga aagcttgtct aatcgtttta atggaaaaca
     8281 ggtctttgtt acagaggata gtcaaattat ccaagaaaaa aatttaactg agaaacaaaa
     8341 agaaattcta gaagattatc gtctgataca atatgatctt gttgcaggta accagtacag
     8401 tgcaacatgg gcgacacaaa aaataaaaga ctaacaaata aactgagcaa ggggttgtga
     8461 taaaagacat gtcacaaccc cttgtttatc tcattaattt gcttgtccat aaacgatttg
     8521 attaaagcca tttttcaatt cattattcgc tgtctcgttg aagtaaagat aattatcggg
     8581 attcccctga ttttgttgtg tcaccaatac ttttggctga ttttgctgaa acccgaatag
     8641 atagacgtgc tttttcagat atggttctgt ctctgcatcg gaatagacgg ctgtgatttg
     8701 atagtctgct tgacctgtcc cactttctga ccattctgca gaaacaggca ctccaccaat
     8761 agccatcttc cactccccgt tcaaaatagc ttgagggact ttaagaccat ataagtccac
     8821 actcatttga ttcgtataac ttttgtattg ttgtcccatt gtttggcccc atttggtcat
     8881 aaatgcctct aattgtgcgg atttgtctgc attccacagt gtttgttcaa cttgttcgct
     8941 gctttctgaa gaggaagatg aagcctctac agaagaactt gaagtagatg tttgagttgt
     9001 ttcagtcgtt ttggaagact ctgacgaagt agaggtatct gctgtttctg tacttgctgc
     9061 agttgacttg ctctctgtgc tagttgttgt agtagttgca actgttgctt tcgctgtttc
     9121 ttttgttgat tcttttttcg attcattccc gttggtacaa ccagctaaca aaaaagctag
     9181 accaattgtt actaaaatcc tttttttca
//
LOCUS       DAFEOM010000093         8970 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.096, whole genome shotgun sequence.
ACCESSION   DAFEOM010000093
VERSION     DAFEOM010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.096"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(57..350)
                     /locus_tag="LP524_002502"
     CDS             complement(57..350)
                     /locus_tag="LP524_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HBL2235502.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(378..1043)
                     /locus_tag="LP524_002503"
     CDS             complement(378..1043)
                     /locus_tag="LP524_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBL2235503.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1183..1815)
                     /locus_tag="LP524_002504"
     CDS             complement(1183..1815)
                     /locus_tag="LP524_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2235504.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1822..2889)
                     /locus_tag="LP524_002505"
     CDS             complement(1822..2889)
                     /locus_tag="LP524_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL2235505.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(2886..3617)
                     /locus_tag="LP524_002506"
     CDS             complement(2886..3617)
                     /locus_tag="LP524_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HBL2235506.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(3786..4265)
                     /gene="greA"
                     /locus_tag="LP524_002507"
     CDS             complement(3786..4265)
                     /gene="greA"
                     /locus_tag="LP524_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBL2235507.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4401..5576)
                     /gene="mltG"
                     /locus_tag="LP524_002508"
     CDS             complement(4401..5576)
                     /gene="mltG"
                     /locus_tag="LP524_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBL2235508.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            5780..6949
                     /locus_tag="LP524_002509"
     CDS             5780..6949
                     /locus_tag="LP524_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBL2235509.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(7216..8850)
                     /locus_tag="LP524_002510"
     CDS             complement(7216..8850)
                     /locus_tag="LP524_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235510.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLN
                     IIQAPWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNM
                     VKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPN
                     KDEITFTLKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTI
                     RYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         2856 a         1702 c         1333 g         3079 t
ORIGIN      
        1 aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct ctttttttat
       61 tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac aagtaaatca
      121 tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc atcaatttcc
      181 actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa accatcatga
      241 acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc tggcagatca
      301 atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat cccaacaacc
      361 tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac atcgcctgtc
      421 tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc aggagaagcg
      481 ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg ttgcctgaaa
      541 tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat ttctgctaaa
      601 gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa attatgcgca
      661 atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat cttttcaagt
      721 actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc catttccttt
      781 accatcagag taactaagat actggcttga atatcttctc caatagcgac aattacatga
      841 tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt tgccacaaca
      901 gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc aatagccagt
      961 acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg tccaagacct
     1021 acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt ctcagtataa
     1081 caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa gaaaaataat
     1141 agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca ccaaaccatg
     1201 tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag ccaatatatt
     1261 ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta tttcctgatt
     1321 actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta gttcttcatg
     1381 taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca cttctggttc
     1441 taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat gtgcggtaga
     1501 ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt tttcgtcatc
     1561 aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga ttgcttttgt
     1621 tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt ccggtcgaag
     1681 tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta cttcaatgtc
     1741 ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt catctactaa
     1801 taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga tttctacact
     1861 tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag ctactcgttc
     1921 tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa accctgttcc
     1981 gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata cctctaattc
     2041 attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc ggaacaagtg
     2101 atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg aaatctggat
     2161 tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt tctcttctaa
     2221 attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag cgttgatgat
     2281 ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt tttctgcttg
     2341 ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa ctgaatcatg
     2401 cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg tttgtccatc
     2461 tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa tcattttttc
     2521 ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg atgcacttgg
     2581 gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac gcagcttttc
     2641 ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga caattaatcc
     2701 tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata aacgtgcacg
     2761 cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga aaagtgaaaa
     2821 tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc tggatatttt
     2881 tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat aatcttcaat
     2941 ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt tttcaaagaa
     3001 aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa tcggatggct
     3061 acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat cacaatatta
     3121 tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga aatgacggcg
     3181 atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc gaataattgc
     3241 tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat catgatctgt
     3301 ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac acctttcaga
     3361 ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt gactaagcta
     3421 aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt acgaggatgt
     3481 ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa aagtccagta
     3541 ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac cacaaaaaaa
     3601 cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc atcataccta
     3661 tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa atctaaaata
     3721 cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta gatctttcag
     3781 tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc tggagtagca
     3841 atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg agaatcattg
     3901 gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta ctcttcttct
     3961 tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc atctgcatct
     4021 acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt tgtgattctt
     4081 ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc ggaaagatct
     4141 ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac tgtttttagt
     4201 tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata aactttctct
     4261 accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct tttgaggaat
     4321 atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct aaaaatgatc
     4381 aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa ttccatatgt
     4441 tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac gaaataataa
     4501 tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg attgtcaaaa
     4561 ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac agctgtatct
     4621 tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat atctgactga
     4681 agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt acgatcattt
     4741 tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg tacagtcaaa
     4801 tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt gttcaccatt
     4861 tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc tgggaataag
     4921 taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa cagttctggg
     4981 aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa aaattctttc
     5041 ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc gtacccttct
     5101 ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc ttcttgcaac
     5161 tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc agcttgaaag
     5221 ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt aataatattg
     5281 tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat ctcgacttgg
     5341 actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata acgatacacg
     5401 gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa aatgatcttc
     5461 ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat atctgctgta
     5521 gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc tgacaaaaca
     5581 actcctcctt actatcaagt tcgtaacttt attcattata catgatattt gaattggcgt
     5641 gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt actataagat
     5701 taaaataaat gttatcaaga tacaagctat tttagcttta ttctttctgt aaaaaagaaa
     5761 ggagactaaa taaactatta tgaaaaaagg aaaacatgct aaaaaaagaa aatattcatt
     5821 tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc tctacaggaa ctattcttgg
     5881 atggcaattt tacaataatg aacactctca gatagttgcc catgctcaat cagctcaaaa
     5941 aaagttgata ctgactacca atgaacaaca gctcgttgaa aaaatcgatc aactattaag
     6001 aaaaaatgat tatattggct caatttacgt aaagaaaaat aatcgcatca tcatggaaaa
     6061 aggctatggc tatgcgaata aactcacgca acaaccaaat gatccttctc tttattatca
     6121 gattggttcg gttcaaaaag cattgactac tcttttgatt ttaaaacaag tcgagcgtgg
     6181 gcacctttct ttagatacta agctggctca attctatcca caaattgctg gcagccagca
     6241 aattactatc cgtgacttgc tgtatatgcg ctcaggcttg caccgaactg cttctccaac
     6301 cataccgatg actgatgaag aagtcgttca atttgcgatt cagcacttaa aatttacaga
     6361 ttatcagacc tctcattatg attcattgaa ctttaccata ttgactggaa ttttgatcaa
     6421 attaacaaat caaccgtatg aaaaattatt gaccgatgat atcatcgaac ctttaggatt
     6481 acagcataca aaattttatg accaagtcaa aaattcatcc aatcatgcac tatcctatca
     6541 aatgtctaaa gaggatgatt actttaaacc actgacagaa tctgaaacag agatccataa
     6601 cgaattaggg actggtaata tcagtatgtc cgtctatgat ttaaatacgc tgtttacaca
     6661 agttttatct ggaaaactag tttccaaaga cttactcttc tcattatggc gtgaaaatgc
     6721 gaatggacat ccatatagtg gtggggtata tagcggagat aattatatcc tgtcgcaagg
     6781 aaatatcgac tcttttgata catctgctat atttagcaaa gattcacagg atgctatcat
     6841 catggaaagc aatataaaag ctgacaaaac tatcaaactg cctactgctg atctaagaaa
     6901 ccaaatctat gacctgatgg aaggaactac taagttggca aatagctgat atgatgaaaa
     6961 gatagcaaat actacttctt tgaatagcat ttgcttaaaa aaagtactcg actaaacgta
     7021 ggaaagagtg tgggacaaat ctaaaaatca atttttgcta cagactttaa ttccaaataa
     7081 acagaacaga aagaggtgtt cttctttctg ttctgtccaa attattctaa gatttctctt
     7141 taccaacatt atttgacaaa gggcctatta aatctaaaat agtatcttta gattgctaaa
     7201 atatgtgcta gcaaatcatt catggcttgt tcttattttt aattttatgc aataaaaaag
     7261 aagaacaatt agcgtgatta tagaaataaa taatgagatt ttagaaaaaa atgtgtatga
     7321 atacctgatt gttactttat ttattccact ttcacttttt actgtaacaa aaggacctcc
     7381 ttgttttagt actgtagcat tttttgaatt aacagtaacc gttaatcctt tataccggaa
     7441 tacaggtaaa actattgttt tgccttttga agatttaagc gtaaatgtaa tttcatcttt
     7501 atttggagaa aagctactat aaataacttg accatcttct gtaacttgat ggtttttcaa
     7561 aagcaattga tcttttttat agcctatagg tacataatca aaattctcaa attcttcaag
     7621 tttattttcc atttgttcat tagaataagt tccttgaaaa ttcatttctt tcaccatgtt
     7681 aactactgat gaataattta aaattgaaat agctaatatt aataatagta taaaatttct
     7741 tctccttgta taagaaaatt tttccaactg tggagaaata aatagagata ctgaaaaaga
     7801 tatgaacaat gttgaaaata tcataaaacg ccaaggagct tgaataatat ttaaaggagt
     7861 attttgtaag gcaaaccatg gaaatatttt tgtacaaagt agaatagata ttatacccat
     7921 tatataagtg attttagata agattcctaa ttttttaaag ttaaatgtta gtataattaa
     7981 acaaataagc aaagttaagc caataccaaa tgatagtaat tctgaatcta tacttccaat
     8041 caaaaactct ttacccagat aaattgctct tttttgtaat atatccacat aaggtctatt
     8101 cagatcttga tacaatgtct gctcaatcat aggacctata aacgctagtg aaagaagtaa
     8161 agtaactata gcagctttta ttaatttaat aattaagaag atttctattt tctttttccg
     8221 ataaaattga ataattaaag caattataat cattaccata gtcatagcta cagaaagaac
     8281 atgagtgtaa attaataaag tcatgctaat cgccaacata acccaagatt ctttttttga
     8341 gtcgtaaagc tttataaacc cgagtaaaat aagaggaagt attgacattg cgagtaattc
     8401 ccccattgcc cctcgaagaa atatttctac actcctaaaa gaagatgttg tatatataat
     8461 agcgaaaaag gagctagtaa catgtttttt ggtaatttca tacattacat aatgacatat
     8521 aaatagagta atgagcgtaa caatataaag aaaaaggtta tagcctattg ctaaattagt
     8581 aaacttgatc aataaaaata aaggatatag agttaaccat gggtaaaaat tatttacttg
     8641 catgccatat gatctaaacg tttcaaaatt aataggggag ctaaaaatag attctaaact
     8701 taacgcacga ttaatgtgaa atgccgtatc ataaaagcca aatatttcca tgtttttaaa
     8761 tgctggaaat aaatataata aactaacaaa tgcataaact ataaatattt ttacagaaaa
     8821 aatttttttc aaattcatat accacctcat gtttttttat attacatata gtatcattta
     8881 tttttttaaa acactagatt agtcactata taaaatgtga ttaattttat tattaataaa
     8941 atatttttta tattacaaaa gtagtgtttc
//
LOCUS       DAFEOM010000094         8708 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.097, whole genome shotgun sequence.
ACCESSION   DAFEOM010000094
VERSION     DAFEOM010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.097"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            7..2199
                     /locus_tag="LP524_002511"
     CDS             7..2199
                     /locus_tag="LP524_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBL2235511.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            2216..2692
                     /gene="ybeY"
                     /locus_tag="LP524_002512"
     CDS             2216..2692
                     /gene="ybeY"
                     /locus_tag="LP524_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBL2235512.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            2676..3071
                     /locus_tag="LP524_002513"
     CDS             2676..3071
                     /locus_tag="LP524_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HBL2235513.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            3086..3985
                     /gene="era"
                     /locus_tag="LP524_002514"
     CDS             3086..3985
                     /gene="era"
                     /locus_tag="LP524_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBL2235514.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            4128..4940
                     /gene="recO"
                     /locus_tag="LP524_002515"
     CDS             4128..4940
                     /gene="recO"
                     /locus_tag="LP524_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBL2235515.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            5324..6241
                     /gene="glyQ"
                     /locus_tag="LP524_002516"
     CDS             5324..6241
                     /gene="glyQ"
                     /locus_tag="LP524_002516"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBL2235516.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            6243..8318
                     /locus_tag="LP524_002517"
     CDS             6243..8318
                     /locus_tag="LP524_002517"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBL2235517.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT         2936 a         1559 c         1849 g         2364 t
ORIGIN      
        1 aagcacatgt tgaataaaac actgcagaaa ctacaagaaa aacttggaaa ggcagcattg
       61 cctttcctgc tgttcttgtt ttttatcatt atctgcggcg ttacatttag tagtgtcaaa
      121 caaaaggcca atgatttcag agaaggccaa gtagcagaag aaagtatccg tgcgaacaaa
      181 acgatcgaga acacggaaga aaccgaacaa aaaagaaaac tggcagcaga agcagtcact
      241 ccagaataca cttaccaaaa agatcttgca gatgaccaga acaatcggat caagcaacta
      301 tttgaattga tcgataaaac gaatgatgcc atcaacaaat cttatgacga aaagattgat
      361 aaggcaaagg aagatgaaaa tatcccaaag cctactacag aagagcggat cgccgcgttg
      421 aagaaaaatt ttgaaaatgt gaatgcagaa aatgttgcgt tttatcaaaa actaccgaac
      481 aatttttatt cgacgatatt tgaaatgact gaaactgaga tccatactgt gagggatgag
      541 agtctgaagc tcattgacga acagatgagc aaacaagttc gtgaaagtga attggaagct
      601 tttaaacaag aagcagaaga gcaaatccaa tatttgaatg tcacaccagc acaacaacaa
      661 atgattcgct acttagtgga tcaaggtatc gtagtgaatg acgtattgaa tgagaaaaag
      721 acagaagaat tgaaacaatc cgcacgagaa gctgtacaac cagtgatgat cttccaagga
      781 gaaatcattg ttcgtgaagg taatcaaatc gatgcttcag ctatgaaaaa attggaatta
      841 ctaggactga ctaatcaaac aacttcgatt tttccgctaa ttgccatggt tttagctgtt
      901 ttgcttcaaa tcggcgtact gatttataat tcacttcaat tccaaaatgc aggagaaaga
      961 acaaaatacg tgcttttcta tgtgacagct atgtcagtca gcatagtgct gatgaaattt
     1021 ttccaattat tccaaacgga acaagctact tatatcccgc tgttttatcc cgctgctttt
     1081 gcaccacttg ttttaagctt tttcttgaac cgaagagcag ggatcatggc tggtattttc
     1141 caagcagtat ctgcactttt cattttttat gattcgattg gcacgaactt tctaacgatt
     1201 atcttgatgt cttatgtttt ctctgggctc atggcaacga ttgtcagacg caaacggatc
     1261 tcagaacaag gattatctgc tgccatgtgg gtcattattt ttcctgtgat gatggatgtc
     1321 attttgatcg tctatcaagg aatgagcttt ggtgatgcaa cgacttggtt aatgcttgtc
     1381 tgcgggttca ctggtgcact cttctcattc ttgctgacga tcggtctaca cccatatatt
     1441 gaattgatgg tcaatgatga cagtgtaatt gtattaaatg aactcagcaa tccaaatcat
     1501 cctttattga aacagttgct ggaagaagcg ccaggtactt atcatcatag tatgatggta
     1561 gctaatctaa gtgctaacgc tgtagcagaa atcggcggac gttctttgct aactcgagta
     1621 gcttgctatt atcatgatat cggcaagatc aaacatgcta gctttttcgt tgagaacttg
     1681 ccttcaggag cggaaaaccc gcacaatttc ttattgccag aagacagtaa acatattatc
     1741 tttggacatg tgacagatgg agccaaaata ttggaagatt ataatatgcc gcaaatggtg
     1801 atcgatattt gcaaacagca tcatggaacc acgctaatgc gttatttcta catcaaagcg
     1861 aaagaacgca atccggaagt aacagaagag caattccgtt atcctggacc aaaaccgcaa
     1921 agtcgtgaag cagggatcgt caatattgct gacagttgtg aggctgcagt acgggcaatg
     1981 gatcatccga cgagcgaaaa aatcgaaagt tttgtccata atcttatcga agagcgtatt
     2041 tccgatggtc aattagacga tagtggactg accttgaaag aaatccgaaa agtggaaaaa
     2101 tcattgatca gcggtttaag tagcaccttc cattctcgga tcaaatatcc taaaatgaaa
     2161 acagaagttg agaagataaa ggaagaacaa gaagaataga aagaagcgat gtataatgga
     2221 tattaccttt atggatgaaa caaaccaagt ctcagaagag aaaatgaaag aaatcgatga
     2281 cttgctgcaa tttgcagcat cttatttaaa gctgccagag gaaacagaaa tgtctgttac
     2341 tttcatggac aatgcggcaa tccaagtaat caatcgtgat taccgtggaa aagatgcacc
     2401 gacagacgtc atcagttttg ctctggaaga agaaggcgaa gacgaaatcc cagtgatttt
     2461 tgaagatgga gagaatccat tgcctcgtgt actaggagac atcatgattt ctattgaacg
     2521 tgctgctgaa caagcagaag cttacggcca ttcatatgat cgggaacttg gctttttagc
     2581 ggtacatgga ttcttgcata tcaacgggta tgaccatatg acaccagaag atgagaaaga
     2641 aatgtttggt ttacagaaag agatcttgga tgcctatgga ctcaaaagat aaaaacgtag
     2701 agaaaaacaa acatttcctg atgtctgtgg aatttgctgt ccaaggagtc aaaaccgttt
     2761 ttgaggaaga acgcaatatg aggaaacatg ttgcgttcgg tatccttgct ttatttatgg
     2821 gcgtgatttt ccagcttgat cggatagagt ggttatggct gcttttagct gtttttctcg
     2881 tttggatagt agagatcatc aatactgttt ttgaaaatgt agtggacatg tttacggatt
     2941 ttcattttca tccgattggg aaaaaaatca aagatatggc agctggagca gttttagtga
     3001 ccgcttgttt tgctgtcatc attgggttga ttttatttgt ccctaaaata tatcatttgt
     3061 tgtttaatta aaaaggagaa cgtatatgcc agaacataaa tcagggtttg tcgcaatcgt
     3121 cggccgcccg aatgtaggaa aatccacatt attgaaccga atcgtgggtc aaaaaattgc
     3181 cattatgagt gacaaggcac agacaactag aaataaaatc caaggagtat atactacacc
     3241 agaagctcag ctgattttta tcgatacacc aggaatccat aaaccaaagc atcgtctagg
     3301 agattttatg gtagaagcag cttacagtgc gttgaaagaa gtggatgcag tcttatttat
     3361 gatcagtgct gatcaaaaac gcggccgggg agacgatttt attattgaac gtctaaaaaa
     3421 cgtgcattct ccagtttatc tgattatcaa taaaatcgat aagatccatc cagacgagtt
     3481 gctgggaatc atcgaagatt attcgacgca gatgcctttt gcacaagtga ttccgatctc
     3541 tgctacagaa ggaaacaatg tcgaacgctt aatggatgta ttagtcagtg aaatgccaga
     3601 gggcccacaa tattttccag atgatcaagt gactgatcat cctgaatact ttatcgtttc
     3661 tgaattgatc cgtgagaaag tcttgttttt aacacgagac gaagtgcctc attctgtcgc
     3721 agtcgttgtt gattcgatga agcgaaacga gaatgataaa atccatatcc aggccacgat
     3781 catcgtcgaa agagatagcc aaaaaggaat cattatcggc aaaggcggaa aaatgctgaa
     3841 gcaagtagga acgaaagcaa gaaaggatat cgaaaatctt ttaggagaca aagtatttct
     3901 agaactttgg gtcaaagtcc aaaaagactg gcgagacaaa aaagtctatc tgcaagactt
     3961 tggctaccga aaagacgaat actgatattc tgaacttctt ataggttaag tatagaaata
     4021 aactggataa aaatccgcac gttgagaaaa acggcggaag atcgaaacat aaataatctg
     4081 gatcggcaga atctgctttt ccagatttat tttttaggag ggagaatatg agccagcttg
     4141 cagaatcgaa aggcttgatt ttatctagcc gtaattacaa agaaaaagat aaattagtca
     4201 aaatctttac ggaatcttct ggcaaaatga tgttttatat caaaggaatc catcggcaaa
     4261 atcagccatt agctccggct ttgctcccct ttaccgaagc agtctatatc ggtaattttc
     4321 gaagtgaagg tttgtcgttt ttaagcagtg tcaaagacgt ccatgcgttt cggaacatcc
     4381 aagaagatat ctttttgagc ggttatggta cctatttgct aaatctagta gatgcagcaa
     4441 tcgaagatca tcaatatgac ccgcatttat tccaatttac gcaacaagct ctgcagcgga
     4501 tggatcaagg agatgatgca gaaatcatca cgaatatctt tgaagttcaa atactgcaac
     4561 gcttcggaat cgcgccaatt tggacgcact gtgtcgtatg cggggaaaca aaaggaaaat
     4621 ttgactattc ttccaaatct ggcggtgtga tatgcgagaa acactggcca atggacgaac
     4681 accgttatca tgcagatcct cgcgctgttt atttcgttcg aatgttttcg gctatttctt
     4741 atgataagat cagtggaatc cacttgaaag aagaaacgaa gcaggcaatc cgtgacctca
     4801 ttgaccagtt gtatgatgaa tatgtcggta ttcatctaaa aagcaaaaag ttcattgatc
     4861 aaatgaagtc ttgggcacac gtattaaaga cgccagagaa aaatatgcct gaaaaagaga
     4921 tacctgaaaa agaagaataa atgaaagata caacaaaaaa atcaaaacta tttgacaaat
     4981 acagggactt ccgtctatta ttaaccgtag attaaataga tcgttcactg agaggatcta
     5041 ttatataaac aagaaaactg caaaatgaat acgtacattg accaagatca gtaaaaaaca
     5101 cgtggtttag cgagttcggg agagtggaag ccggacccaa acgatttttg aagaggcact
     5161 tgcgagtacg ataaatgaag ctgccgaatc tttattcgga agtagggtgg aaccgcgaaa
     5221 ttattcgtcc ctatgtctgt aatcagacat agggactttt tgtcgttttc tttcctgtat
     5281 tcttattgac agcaacgctt aaaaattgaa aggggcaaaa cagatgagta aaaaattgac
     5341 cgtccaagat atgattttaa cattacaaaa attttggtca tcaaacggct gcatgctgat
     5401 gcaagcttat gacacagaaa aaggagcagg tacgatgagc ccatatacat tcttacgggc
     5461 aatcggaccg gaaccatgga atgctgctta tgtagagcca tcccgccgac cagctgacgg
     5521 gcgttatgga gaaaacccaa accgtttgta tcagcatcat caatttcaag tcgtaatgaa
     5581 accttcacct gaaaatatcc aagagcttta tttagaaagc ttgcgtcttt taggaattga
     5641 tccattagaa cacgatattc gttttgttga agacaactgg gagaacccat cgatggggtg
     5701 tgccggtgtg ggttgggaag tatggcttga tgggatggag atcacacaat tcacgtactt
     5761 ccagcaagtc ggaggactac cttgtaaacc agttacttct gagatcactt atggactaga
     5821 acgtcttgct tcatatattc aagaagttga aagtgtctat gatctggaat gggctgatgg
     5881 tgtgaaatat ggcgagatct tcaaacaacc agaatatgaa cattccaaat attcttttga
     5941 agtaagtgac caagacttgc tactaagcaa ctttgaccgt tttgaaaaag aagctaaacg
     6001 ctgtatcgat gaaaatcttg ttcatccagc gtatgattat attttgaaat gcagccatac
     6061 attcaattta ttagatgctc gcggtgcagt atccgtaaca gaacgagcag gttatctttc
     6121 ccgtatccgc aacatggctc gtgctgtagc caaaatcttt gtggcagaac gcgaaaaatt
     6181 aggattccct ttattgaata aaaacacagc aaatccagaa aacatgaagg aggctgaata
     6241 agatggcaaa agacttatta cttgaaatcg ggctggaaga aatgcctgct catgttgtca
     6301 caccaagcat gaaacaatta gaagaaaaaa cagcaaaatt tttagatgaa aaccaattga
     6361 cttatgatac gatcgaaacg ttttctacac cgcgccgctt agcaatcaaa gtaacaaaca
     6421 ttccagaacg tcaagcagac agtgaagaag aagtcaaagg accagccaaa aaaattgctt
     6481 tggacgcaga tggaaactgg acgaaagcag ctgaaggatt tgtccgcgga caaggtctga
     6541 cgacagatgc tatcactttt agagaattaa aaggcgtgga atatgtctat gtaacgaaat
     6601 acactcacgg caaagaagcg aaagaagtat tgacaggttt agcagatgtc attaaaagcc
     6661 tcactttccc agtaacgatg cattgggcag aatacgattt tgaatatatc cgccctattc
     6721 actggatcgt ggcattgtta gatgacgaag taatcccgtt tgagatctta gatgtcaaaa
     6781 caggcagagc ctctcgagga catcgtttct taggcgaaga tgtgacattt gcgcatgcaa
     6841 aagaatatga agaaaaattg aatgaacaat tcgttattgc ttcaccgcaa aaaagacaag
     6901 aaatgatcgt tacacaagca aatgaattag ccgaaaaaaa tcactgggtt attgatctag
     6961 acgaagattt actagaagaa gtcaacaatt tagtcgaata tccaactgtc ttttctggcg
     7021 acttcgaaga aaaatatctt tctgttcctg aagaagtact agtcacttct atgaaagaac
     7081 atcaacgtta tttcgaggta cggaacgatc aaggtatgct gcttcctcat tttatttctg
     7141 ctcgtaatgg tgatcgggta aaattggaaa atgtggttcg aggcaatcaa aaagtattga
     7201 cggctcgttt agaagatgcg gaattctttt ataatgaaga caagaaagct tcaatcgacc
     7261 aatacgttga aaaactaaaa actgttactt tccatgaaaa aatcggtagt atctatgaga
     7321 aaatgcagcg ggtacaagtc attgcccgta tcattggcga acaagtagga ctatctgagg
     7381 aagaattgat ggatctgcaa cgagctgctg aaatctataa atttgatcta gtaaccaaca
     7441 tggtagggga atttcctgaa ctacaaggga tcatgggcga aaaatatgct ctgctgaaag
     7501 gcgagaaacc tgctgtagca acagcgattc gtgagcatta catgcctata tccagtgaag
     7561 gagagctacc tcagtctaca gtaggttctg ttttagcgat tgcagataaa ttagatagca
     7621 ttttttcttt ctttgcagtt gggatgattc caagcgggtc aaacgatcct tatgccttac
     7681 gtcgccaagc atacggtgtc gttcggatca tcgaatcgaa aaaatgggcc tttcctttgt
     7741 ctgttctgca agaaacgatt agtgatgtta tcagtaaaga tacggatcgt ttcggcattg
     7801 gtttatcaga agggcaacaa caggtgatcg actttatcaa aggccgtctg cgccaattat
     7861 tgacaacgaa gaatattcgt catgatgtca tcgaagctgt tttgaatgca gaacaaaaag
     7921 atctgacaaa agtatttgca gcagcacaat tgttcaagca gcatcttgca gatgaagact
     7981 tcaaaccatc gatggaagct ttgacacgtg tcgtgaactt agcgaaaaaa gcggagcttg
     8041 aaaaacaatc agaagtagac cctgaactgt tcgaaaatga agcggagaaa gaactgcaca
     8101 aagcagtaga ggcaatgaaa aaaagctttg ctgaccaaac gatcaacgaa aattatgatc
     8161 agcttgtaag ccttcgccca ctgatcgaaa attattttga tcagacgatg gtcatggcag
     8221 aagaaccagc agtacgtaag aaccgattaa cgcaattaag tcaaattgct gccatggctt
     8281 tgtcgctagc aagtttagat caaatcatca caaaataatt tctaatatgt aaataaacca
     8341 gagaataggc acaaaatagg ctatttctct ggtttatttg ttataaatat aaataactat
     8401 acccgtggtg ctagttaatt ttttcctagg tattatacaa gtaatttgag tcggtcaatt
     8461 tcaactgaat tatctaaact aactgatcta cttttagata attcagttga aatttattgg
     8521 taaactgtcg cttttctttt taaatggcaa aaatttttaa taatatatga caatttagaa
     8581 atcaactata agactgatta attcaaaatg atcttagaat cattgtatga acactaaata
     8641 ctatattgtt ttctattaat cgttatataa acgattatga attcaaagct actttttatg
     8701 acgtcaat
//
LOCUS       DAFEOM010000095         8493 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.098, whole genome shotgun sequence.
ACCESSION   DAFEOM010000095
VERSION     DAFEOM010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.098"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(<1..173)
                     /locus_tag="LP524_002518"
     CDS             complement(<1..173)
                     /locus_tag="LP524_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL2235518.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="LP524_002519"
     CDS             complement(593..1714)
                     /locus_tag="LP524_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HBL2235519.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="LP524_002520"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="LP524_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HBL2235520.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="LP524_002521"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="LP524_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HBL2235521.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="LP524_002522"
     CDS             complement(4304..5215)
                     /locus_tag="LP524_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL2235522.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="LP524_002523"
     CDS             complement(5232..6239)
                     /locus_tag="LP524_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBL2235523.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="LP524_002524"
     CDS             complement(6236..8365)
                     /locus_tag="LP524_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL2235524.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8493)
                     /locus_tag="LP524_002525"
     CDS             complement(8370..>8493)
                     /locus_tag="LP524_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL2235525.1"
                     /translation="TGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1487 g         2679 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg gtt
//
LOCUS       DAFEOM010000096         7930 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.100, whole genome shotgun sequence.
ACCESSION   DAFEOM010000096
VERSION     DAFEOM010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.100"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            143..847
                     /locus_tag="LP524_002526"
     CDS             143..847
                     /locus_tag="LP524_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011476586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBL2235526.1"
                     /translation="MTKHTHHEIGDHAACIRLVPIFNHLEDSAMNYIAEKAHTKQYQK
                     GEFLFRSQEKEDTLYIINRGKIRIYRLADSGKEQLVRILNPGDFTGEWTLFNPDAVHE
                     EYAEATRDTVVCMIQQKDVQDFLEQYPAISLKLLAEMSKRLESSERQTTQVAVESVIT
                     RLVLFLAENVEPEMGNSPTITLPMAKKDIASYLGTTPETISRKFGELEDEGLIQQLSG
                     KRIKIQDLDDLLLYSE"
     gene            1075..1413
                     /locus_tag="LP524_002527"
     CDS             1075..1413
                     /locus_tag="LP524_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235527.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            1398..2252
                     /locus_tag="LP524_002528"
     CDS             1398..2252
                     /locus_tag="LP524_002528"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBL2235528.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            2399..2632
                     /locus_tag="LP524_002529"
     CDS             2399..2632
                     /locus_tag="LP524_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HBL2235529.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            2830..4734
                     /locus_tag="LP524_002530"
     CDS             2830..4734
                     /locus_tag="LP524_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBL2235530.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISVGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPTEKVKIS"
     gene            5088..5453
                     /locus_tag="LP524_002531"
     CDS             5088..5453
                     /locus_tag="LP524_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2235531.1"
                     /translation="MTAEVCTTTCIHKEKVASVKNNLKDKDLSTLLVLGKCFSDSSRI
                     KIFYALETYKEMCVCDLAAILNASIATTSHHLRFLKKNGMAKSRQYGKVVYYSLANEE
                     ILSAVQAFLKLSENSSMKA"
     gene            5470..6081
                     /locus_tag="LP524_002532"
     CDS             5470..6081
                     /locus_tag="LP524_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HBL2235532.1"
                     /translation="MLQNILSALAVYISTSIDYLFILLIIFSQNHTKKGLRQIFFGQY
                     LGTGILVAISLFAAYVLNFIPQDWIIGLLGLIPIYLGIRVAFVGEEEEEEGEVVEKLG
                     SRGTNRLFWTVSLITIASGGDNLGIYIPFFTSLSFSEIVTSLIVFAISVAVLCYISYK
                     FAKISFVSETLEKYERIIVPIVFIGLGIFIMIENGTIQTILGL"
     gene            6109..7770
                     /locus_tag="LP524_002533"
     CDS             6109..7770
                     /locus_tag="LP524_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBL2235533.1"
                     /translation="MSENKKYHEWILEGIDCANCADKVERGVAKVAGVANSSVNYMTE
                     TLSFEVTEEKEYLVLSNVQQKVKKLEPDITLKNKVDGRLIEINPALIKKSNLKDDKKN
                     YEWILEGIDCANCAAKVERGVATVPGVTNSSVNFMTETLSFEVAGENKTDVLASVKQT
                     VGTLEPDVVLRKKSDGTLIEHGTDSNKNSTSNKSISDKDETNQKQTNRAYLASLLGNK
                     TKITLIRLILGLGILATGIFLPMDGQASLGLFILAYLITGYDVVGRAIRNIFRGQVFD
                     ENFLMTIATLSAFYIQEYPEAVAVMLFYQVGEVFQDIAISKSRRSIADLMDIRPDYAN
                     LVTGNNVEKVAPEAIKIGNIILIRPGEKVPLDGKVVEGASAMDTSALTGESVPRSVKP
                     EDTILSGFINKNGLLKVVVEKPFSESTVKKILDLVQNASGRKAPTEQFITKFSRYYTP
                     IVVIAAALLAIVPPLLVPGATFNEWIYRASIFLVISCPCALVVSIPVGFFGGIGSASR
                     KGILVKESNFLEGLNDLKYVVMDKTGTLTKGFCCKVLNLLSNKVE"
     gene            7798..>7930
                     /locus_tag="LP524_002534"
     CDS             7798..>7930
                     /locus_tag="LP524_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBL2235534.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     "
BASE COUNT         2506 a         1294 c         1766 g         2364 t
ORIGIN      
        1 taaaagtaga agctgattta gggattggaa ttctttccag tcctttttcc attttcttga
       61 tgtgcatcaa tttttttgta gtaagagact tatataataa aagcagcgaa tataaacaaa
      121 ataaataaag ggggagaatg aattgactaa acatacccat catgaaattg gcgaccacgc
      181 agcctgtatc cgcttggttc caatattcaa tcacttagag gatagcgcga tgaattacat
      241 tgctgaaaaa gcccacacga aacagtatca aaaaggagag tttctgtttc gttcacaaga
      301 aaaagaggat actttatata ttatcaatcg tgggaaaatc cgtatttatc gattggcaga
      361 ctctggcaaa gagcagctag tgcggatctt gaaccctggt gatttcaccg gagaatggac
      421 cttgttcaac ccggatgcag tacacgagga atatgccgaa gcaacccgag ataccgttgt
      481 ctgtatgatt cagcaaaaag atgtccaaga ttttctggaa caatacccag ctatttcttt
      541 aaaactattg gcggaaatgt ctaaacggtt agaaagttca gaacgtcaaa cgacacaagt
      601 agccgtggag agtgtcatta cccgtttagt tttgtttttg gcagaaaatg tggaacctga
      661 aatgggcaac tctcccacca tcaccctgcc gatggcaaaa aaggacattg cttcctattt
      721 aggaacgaca cctgagacca tcagtcgcaa atttggagaa ttggaggacg aaggattgat
      781 tcaacagctg tctgggaaaa ggatcaagat tcaggattta gatgatttat tgctttacag
      841 cgaataatcg gacacacttt tttctacacg ttggtgttct gtcgctcttc attaacaacg
      901 ggacagaact cagtgctttt gcttccatct gtccgttttt ggctttttta agacagaacg
      961 accttcattc attggaaaaa taaaagcaca aacatgataa ccctcaagtt tttttgtttt
     1021 atttttcttt aagatagggt tgtcgaacaa aacgtaaaaa taagtagggt gaatgtgcat
     1081 tgtttttggg tgatagttgc catcacgttg tcagcagcgg gggcattctt tgtcgtccct
     1141 ttcaccaaga acacgattgg cggcgaagca aaaacgtcta acgttgttga ggcagatagg
     1201 ttagctatgg aaaaagaaga ggaagttgta aaaggagccg gcgtaatcgg acacggaaaa
     1261 gtggcgtatt acgaagcgag tgaccaggga gataaaagtg tattcactgt ttggtttcca
     1321 tggagcttag ccggaatctt gttcagcatc accatgacta gaatgacgcg aaaaattgaa
     1381 gaaatggagc aaatagaatg ggattcgtat taggcgtttt gaatccccat gtggtggaga
     1441 ttggttctgt tgtgattcat tggtatggag tcattattgc agcgggcata ttagcggctc
     1501 tccagttgag caccaaggaa gcaaagaaaa aaggactgga agaggatacc attatcgata
     1561 tggccttatg ggcaattccg attggactcc tcggcgctcg gctttattat gtgttatttg
     1621 aacttggtta ttatctgcaa aatccaggac aaatccttgc gatttggaat ggcggcattg
     1681 cgatttatgg gggattgatt gcaggagggg gtacgctata ttggtatgca aagaaaaaag
     1741 gtacttcttt ggcactcgtt cttgatattt tagcgcccaa tgtattgtta gcccagtcca
     1801 ttggtcgttg gggcaatttt atgaatcagg aagctcatgg tgggccggtt actcgtcaat
     1861 tcttggaaaa tctttattta cctgaattta tcattgaaca aatgaatatg aatggtacct
     1921 actaccatcc gactttttta tacgagtcat tgtggagttt gttgggcttt gtcctcatcg
     1981 tcactatacg gaaccgaaaa cagcttttgc gtcaaggaga agtcgctttg agttacgttt
     2041 tgtggtactc agtgggtcgg ttcttcattg aaggtatgcg aacggacagt ttatggattg
     2101 gcgagtggat ccgcgtttcg caagccttgt ctctggccct gtttattggc gcgattgtag
     2161 tatggtttat acgcagacaa gattatccac caatttctta ttattctgat ggcaataagg
     2221 ggcagaaaaa gactattaag atggtgaatt gagcaaaaag ataaaaagaa gtgcaaaact
     2281 tgatatgcgt caattttcgt ttgctaaaag tgagctatac tacagttgta aagaggaaaa
     2341 ggcagtgttc attaatctgc tgccgataaa ataaaaacac ataaaggaga taggaattat
     2401 ggaaaaagct atattgcaat tagaaacgtt gtcttgtcca agttgtatgc aaaaaattga
     2461 aggtgccgtg aaatcagtta acggtattga taaagacagc attaaagtat tattcaactc
     2521 cagcaaagtc aaaaccaatt ttgattcagc tgtcacttcg attgaagaga ttcaaaaagc
     2581 tatcgaaaac gtaggctatc cggttcttaa agcaaaagtc aaggccgttt aacagattaa
     2641 atgaactagg caattctgga agaaaagcta tttttccagg attgcttttt ttgttgcaat
     2701 ccgattcgag gtgcttagaa acgataaaag agaactttct aaacttgatg tcagtcaatt
     2761 aattacttag gcaattatct tatactgtgt ttatcaataa aagagaaatt aaatttggag
     2821 ggaaaaagca tgcaacaata tatattaagc aagaaaaatg ttatcaccgt catcagtgga
     2881 ttgttaatcg tactcggttt cttcagtcac tttgttttag aaaacgtagg actttcagag
     2941 tggtctttga tcatcgcctc tgtctttggg attacgccaa ttgccattca agcgtttcaa
     3001 gcaatgaaag tcaaagtcat cagtattgat gtcttagtca gtattgcagc gattggtgcg
     3061 ctttttattc aaaattatga agaatcggct attgtcacgt tcttattctt attcggacac
     3121 tacttggaac aacgaacatt gaaccaaacg cgatctgcta tcaaagaatt gactgaaatg
     3181 gcacccgaaa gcgccttgaa acaaatggat aatggcgaat ttgaagaagt agaagtggat
     3241 gatatagatg aaggggatat cttgctcgtt aaaacgggtg cgaaagttcc agtagatggc
     3301 atagtcctta cgggtgaagg gcatatcaat gaagctagca ttacaggtga agctattccg
     3361 gtcaacaagc tagctgatgc agaagttttt gcaggaacca ttttagaaaa cggaacgatt
     3421 caaatcagag ctgaccgagt tggtgaggac accacatttg gaaaaattat tgaactcgtg
     3481 gaagaagcac aagattctaa atccgaagcg gaacggttca ttgatcgctt ttctaaatac
     3541 tacacaccag ctgtcttggt tctggcaatt gttgtatggg cgttcagtca aaatattgag
     3601 ttagcaatca ccatcttggt tctaggttgc ccaggcgcat tagttatcgg agtgcctgtc
     3661 tcaaacgttg ccggtattgg gaatggtgct cgtaacggtg ttcttttaaa agggagtgaa
     3721 gtcattcaag acttcagtaa tgtggataca attgtatttg ataagacagg tactttaact
     3781 gtcggaaacc caaccgttgc agcgactgaa ctgtatgaaa aagatcctgc tgaaacgctt
     3841 ggctatctgg ccagtgtgga acgggaatca gatcatccat tagcaaaagc ggtcttaaat
     3901 caaattggag aaacaaactt ttatcctgtt gaaggaactg aagttgtgaa aggcggcgga
     3961 atcgtttcaa gtgtagctgg acatagagtg gctgttggga atgtggcctt gatggaaaaa
     4021 gaaaatgtca ctctcagcaa aaaagtgcaa aaagatgtca aacggtttga acaacaaggg
     4081 aactctctcg ttttgacagc ggtcgacggt gaattaaaag tactgatggg cattcgcgat
     4141 caagtccgtc cgggtgtgaa agctaatttg caggaattaa aagacttggg cgtgaaaaat
     4201 ctagtcgttc tttcaggaga taaccaagga accgttgatg tggtcgccgg cgaacttggt
     4261 ttgaccgaag ctcacggcta catgttgccg gaagacaaat cggcttatat cggaaaactt
     4321 caacaacgtg gtcaaattgt agcctttgtt ggagatggcg ttaacgatag tccgtcctta
     4381 gctttagcag acattggaat cgcaatggga agcggaacgg acgtagcgat tgaaacatcc
     4441 gatgtcgttt taatgaactc gaacttcagc aacttgcctc acgcattagg attagtaaaa
     4501 gccaccgcaa ataatatgaa acaaaatatc gtgatttcag ttggagtagt gttggtcttg
     4561 ttgaccagcg tcttcttcag cgaatggatg aatatgtcta tcggaatgtt ggttcatgaa
     4621 ggtagtatct tggtggtaat tttcaatggc atgagattaa tgaagtataa gttgaaaggt
     4681 cggaaagaac aaaaagaagt atcagcacct acagagaaag taaaaatatc ttaagaataa
     4741 attagaagag gctgagacaa tagtccatcc ttgaaatgac aaaagcggaa actcagattt
     4801 ttaaatctgg gattttccgc ttttttgagt tcttgaaaaa aaataagagt atagagtatg
     4861 ttctttataa aattaatttt cgtgtaaatt attaaaaatt taataataaa atttaaattc
     4921 ctattaagtt gtaacacatg aaattgctta aaatagtgta tcaaaaacct tgttttatta
     4981 atgttgaatt tatttgaaag gtctacagat taacatatta ttgacaaaac aatatttaaa
     5041 tagttatact tcattcaaac gtctgtttga acgaggtggt gaagttaatg actgcagaag
     5101 tatgtacaac tacttgtata cataaagaaa aagtagctag tgttaaaaat aatttaaaag
     5161 ataaagacct ttcaacttta cttgtattag gaaaatgttt tagcgattct tctaggatta
     5221 aaattttcta tgctttagaa acgtataagg agatgtgcgt ttgtgattta gcagcgatac
     5281 ttaatgctag tattgctaca acgtcacacc atttacgttt tttgaaaaag aacggaatgg
     5341 cgaaatcacg tcaatatgga aaggtagttt attactcttt agcgaatgaa gagattcttt
     5401 ccgctgtgca ggcttttcta aagttgtcag aaaatagttc tatgaaagct tagtttggag
     5461 gtagttattt tgttacaaaa cattttatca gcactcgctg tatatatttc taccagtatt
     5521 gattatttat ttatcttgct gattattttt tctcaaaacc atactaaaaa aggtctccgt
     5581 cagatttttt ttggtcaata tcttggaaca ggaatcttgg tagccataag tttgtttgca
     5641 gcatatgtgc taaattttat tcctcaagat tggatcattg gtcttctggg actaattccg
     5701 atttatttag ggattcgtgt tgcttttgtg ggcgaagagg aggaagaaga aggggaagtc
     5761 gttgagaagc taggatctag aggaacgaat cgtttgtttt ggacagtttc cttaataacg
     5821 attgcttctg gtggcgataa tttagggatt tatattcctt tctttacctc gttatctttt
     5881 tcagaaattg tcacttcttt aatcgtcttt gctatctctg ttgcggttct ttgctatatt
     5941 agttataaat ttgcgaaaat ttcttttgtc tctgaaacac tagaaaaata tgaacgaatc
     6001 attgtaccta tcgtatttat tggattaggg atttttatta tgatagaaaa tggaaccatc
     6061 caaactatat taggtttgta gagatcttat tagtaaagga agcaaacgat gagtgaaaat
     6121 aaaaagtacc atgaatggat attagaagga atcgactgtg caaattgtgc agacaaagta
     6181 gaaagaggcg tcgccaaagt tgcaggtgtt gccaatagta gtgtgaacta tatgacagaa
     6241 accttgagtt tcgaagtgac agaggaaaaa gaatatcttg ttttatccaa tgtgcaacaa
     6301 aaagtaaaaa aattggagcc tgatatcact ttaaaaaata aagtggacgg taggctgatt
     6361 gaaataaatc cggcattaat caagaaaagt aatttaaaag acgataaaaa aaactacgaa
     6421 tggatattag aaggaatcga ttgtgcaaac tgtgctgcca aagtggaaag aggagttgct
     6481 acagttcccg gcgttacaaa cagcagcgtc aattttatga cagagacact gagcttcgag
     6541 gtagcaggtg aaaataaaac tgacgtgttg gctagtgtaa aacaaacagt aggaacttta
     6601 gaacctgacg ttgttctgcg aaaaaaatcg gacggtacat tgattgaaca tggaactgac
     6661 tctaataaaa attcgacttc taacaaatcg atttctgata aagatgagac aaaccaaaag
     6721 cagacgaata gagcttatct ggccagcctt ctcggaaata aaactaaaat tacccttatt
     6781 cgtttaatac taggattagg aatattagcg acaggaatat ttctccctat ggatggacaa
     6841 gcctctcttg ggttgttcat tctggcttat ttaatcaccg gttatgacgt tgtaggacgt
     6901 gccattagaa atattttccg aggtcaagta ttcgacgaga actttctaat gaccattgcg
     6961 acgttaagtg ccttctatat tcaagagtat cccgaagctg tagcagtcat gctattttat
     7021 caagtgggag aagtattcca agatattgct atttcaaaat ctcggcgctc cattgcggac
     7081 ctaatggata ttcgacctga ttatgccaat ttagtcacag gaaacaacgt agaaaaagtt
     7141 gctcctgaag caattaagat tggaaatatt attctcattc gtccgggcga aaaagtgcca
     7201 ttggatggaa aagtagtgga aggtgcttct gcgatggaca cttctgcttt aacaggcgaa
     7261 tccgttccaa gaagcgtgaa gccggaagac actattttga gcggatttat taataaaaac
     7321 ggattgctaa aagtagttgt agaaaagccc ttttcggagt ctactgtgaa gaaaatttta
     7381 gatttggtcc aaaatgctag tggccggaaa gcacctactg aacaattcat taccaaattt
     7441 tctcgctact atactccaat tgtggttata gctgctgcct tactggcaat tgttccgcca
     7501 ttgctagtac caggcgctac ttttaatgaa tggatatata gggccagtat ctttttagtt
     7561 atttcatgcc cttgtgcttt agtcgtttcg attccagttg gtttctttgg tgggattggt
     7621 tctgcttccc gcaaggggat tttagtaaaa gaaagcaatt tcctcgaggg tttgaacgat
     7681 cttaaatacg tggtaatgga caaaacgggt acattgacaa aagggttctg ttgcaaagtt
     7741 ttaaatctac tatcaaataa ggtagaataa tagaaaaaga tagtaggagg aatgacgatg
     7801 aatcatttta aaggaaagca atttcagcag gatgtgatta ttgtagccgt gggatactat
     7861 cttcgttata accttagcta tcgtgaagtt caagaaatct tatatgatcg tggcattaac
     7921 gtttctcata
//
LOCUS       DAFEOM010000097         7928 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.101, whole genome shotgun sequence.
ACCESSION   DAFEOM010000097
VERSION     DAFEOM010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.101"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(140..811)
                     /locus_tag="LP524_002535"
     CDS             complement(140..811)
                     /locus_tag="LP524_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBL2235535.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQQEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            complement(897..1091)
                     /locus_tag="LP524_002536"
     CDS             complement(897..1091)
                     /locus_tag="LP524_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235536.1"
                     /translation="MEEKLLKTMKQKHLKRLSVMQYINDMQITGKEKACLLGSMKNFE
                     QLRRTYVKTSSNCQLLLEVS"
     gene            complement(1203..1442)
                     /locus_tag="LP524_002537"
     CDS             complement(1203..1442)
                     /locus_tag="LP524_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235537.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            complement(1556..1897)
                     /locus_tag="LP524_002538"
     CDS             complement(1556..1897)
                     /locus_tag="LP524_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HBL2235538.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYYQKLTDNPIGFGLLPEDDEGNEWFKMTLTNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            complement(2516..2848)
                     /locus_tag="LP524_002539"
     CDS             complement(2516..2848)
                     /locus_tag="LP524_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBL2235539.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYAGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            complement(3695..4735)
                     /locus_tag="LP524_002540"
     CDS             complement(3695..4735)
                     /locus_tag="LP524_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBL2235540.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFIPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAETESGISIIFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNSE
                     SFPKTITKDWLE"
     gene            5901..5987
                     /locus_tag="LP524_002541"
                     /pseudo
     CDS             5901..5987
                     /locus_tag="LP524_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            6547..6765
                     /locus_tag="LP524_002542"
                     /pseudo
     CDS             6547..6765
                     /locus_tag="LP524_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            7025..7279
                     /locus_tag="LP524_002543"
     CDS             7025..7279
                     /locus_tag="LP524_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL2235541.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLIILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            7280..7597
                     /locus_tag="LP524_002544"
     CDS             7280..7597
                     /locus_tag="LP524_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBL2235542.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT         2580 a         1389 c         1151 g         2808 t
ORIGIN      
        1 tgctcgatat ccctgaaagc agcctgcaat gattcctaac attccaataa gcattccagc
       61 tttttttaca taccgattta ttgccatttt ttactctcct catttcatat ttcaacgact
      121 cacgttgatt aaaaccttgt tattgtagtt ctccctgtac cacgtatctt tcttttcctt
      181 gtgacgtata aacacaagta aacaacgtta ataagggttt ctctcctttc tttggcaaag
      241 acaagaaaga gttctcttca ggaacgccat cgattaattt aaagtactca ggtacgataa
      301 atttacgcgt taccttgtaa cgatagacgc catcgttact aactaagtcc atcacctccc
      361 ctaatgacaa cttttgaata tcagaaaata aaacacctgg catttccatg ttatgacctg
      421 ctaggacata gtttcctttg cccatttcag catcttcata aaaataagta gccgctccaa
      481 gactcaaagt gtattgattt gcccctttaa aaattggcaa tgctaaacca agtgcaggaa
      541 tctgaatttg tgcaaatact tgtttattga cgcccatttc gattgcttct tgttgtatgg
      601 acaacatttg ccccaaagac atatcattat tgacgttcgt tgcatcctcc ttataactct
      661 ttgcatgtgc attgatttga tctacgggca tctttttttc tatattttgc ttaaacacct
      721 ctccttcatg tttttggtag ctaacatagc caaacacact gcaaaagacg attaaagcga
      781 ctcctaaaaa accaaataac cattttctca tccagctacc ccctttcaga caaaacaaaa
      841 aatctttttc tcatttccat agaaaaagtt ttaaaaatga attctattaa tgaatgtcat
      901 gagacttcta acaatagttg acaattagaa cttgttttca cataagttcg tctcaactgt
      961 tcaaagtttt tcattgaacc taacaaacaa gctttctctt ttcctgttat ctgcatatca
     1021 ttgatatatt gcatgacaga taatctttta aggtgttttt gtttcatcgt ttttagcaac
     1081 ttttcttcca tttatttcga cacttttttt ctgttttcga tttcttcata attcatgagt
     1141 tttcctacct tccttgtttt ttgcaaaaat aaaaaagcct atgatcacat acgctctaca
     1201 cttcagtctt gggttatctt tttctttcgt ttaattactg gccgtgctgg tttcaaatcc
     1261 ttgattccat tatgggttct ccaataatcg aattcttttc gtaatctttc tttttccata
     1321 tctgttcctt tagacaggga tttgaataca tctttaggtg aataaccacg agctaacagc
     1381 tgtacatacc ttccttttgt caaagtatcc atatccatcg cctctaaaac tggtcgcgcc
     1441 atctaatttt cctcctaaat gaattcctta ctcaatgcct aaggtaatac taccttttta
     1501 ttattcgtcg tgacttcttc taagccttta tctgtaaaat ttattcctcc agtttttatt
     1561 cttctttatc cgctacaaat tcaatgattt caacgctgac aatataatca ctcaaatatg
     1621 accaagtatc ttcaacagat aattcgtctc cattatcatt cgtaagcgtc attttaaacc
     1681 attcattacc ttcatcatct tcaggtagca agccgaatcc aattggatta tcagtcaatt
     1741 tttgatagta taaatcatcc tcgtctaatg ctaaaacatc taatccttta caatttccgc
     1801 ctcttctaaa cgtcacaaat cctttgtaat cccaacgttg gaaagaaacc ttaatagtat
     1861 gtgtgcccaa tcgtatattc ggatcataat ttttcatttg tcattcctcc attacgtaaa
     1921 tttcattttc tctatgtttt atatcaattt tttcacttta ttcaggactc gttcatattt
     1981 tatcgttttt cgtttactta aaaatacata aaaaatatcg actcgtttat ggcgtccgaa
     2041 acgagaaacc ctagtagaaa tcggtaaatg ctcgattgat ccaatgacca acgtcctcgc
     2101 aagtatctac atgtactgca gcacacgaca caatttcttg tgcgtcctcc ccattacgag
     2161 aagacctact ccttattcca tgagtttgcg tcaaagccta gatcccttag tagctgctca
     2221 tgattaatct aggcgctatt ctaacttgtc taacttctaa attccctctt gtttactctg
     2281 tgcatattat ttacacaaat tcatttcacc actatccaaa caagggcatt tactcgattg
     2341 accaattttt tacgttacca atcgtaaaaa cctaccccgt caccccccga aaggaattgt
     2401 tgatagatca agtctactcc ttatttttct agtaattcta agttctttta gaagtctcga
     2461 caatattttc aagcaagcga aaaacgataa aatacaagtt atgagaatga tcaattcagc
     2521 tgattaaacg ccccatccct tttgcggtac aacgcaagac tccatttttt cttcgaccaa
     2581 tcagtacacc gttttggtca taaacatgtt tgattgttgg atatagttct tttaaaggga
     2641 taggatcttt taaaattatt tgtttttgtt taccagcgta ataaatcatt tcctctgaga
     2701 ctttgtcttt ctggattata tatttcttac actgcacaac ggttttactt tggccagttg
     2761 atagtttctt cgtgattttc tctgtagcgg tacaccgttt accgtagtga caaatatctt
     2821 cgagactcaa aattcctgta tatgacataa aataatccac ccacttactt taaatatttt
     2881 taataagttt ttcttgttat ttttcaaaaa tagagaattg attttcgttc atgacaaaca
     2941 aaaaagaggg aacgcatcca aacatgcgtt ccctctaatt tcagcgttcg tcatacggca
     3001 aagagccaca cggacatttt tataacatta tgcttaggca tagattcatc gtaatatcac
     3061 gtatttttaa ccttaacaca gccaaaaaaa gtggtatcac ttttgaatat ctgttatact
     3121 aaatccgttg cgggtaatac cgtaacactg aaaggtaaac gcattaaggg attaaaggtt
     3181 gtcttaatgc cacctgtgga cgtcgacgaa ggttaccagc cttccgagtc tcacagggcc
     3241 tttctttttt ttgtattttc aattgtccaa atttatcgtc caaaagtgca gaacgaaaat
     3301 taattcatca caagcgtagc agacaattaa atttaaaaca actaaattta tacaaaaaaa
     3361 tgtaagcgaa taatattcgt attgcctgaa aagaagtaat atcaaggttt ttagcttttt
     3421 taaaaaatga aattacctct cttcgcgtat tattataact aaaaaacagc aacatgcaaa
     3481 cgtttgttct cttttttgtg ttggaaatta attattaact attaacactt atgacgtaaa
     3541 aagctacgtt tgtgattttt atcataatat tttaactcat aaattctatt aaaatagcta
     3601 tttctttgta acatttgtaa tataattaga ataataaaaa atttttaatt ttaatgctac
     3661 tccatgaagc aacaatcaat ttgtcagtga aatttcactc taaccagtct ttcgtaatag
     3721 tttttgggaa gctttcagaa ttcaaacgtt tttcacttga tactttagca gctattggtt
     3781 tttcgataaa ccaatttttt acatagacaa aaatcaggtt ctggatattt tcaacttttt
     3841 cggtttttaa tttttgatag gcttttaaca gtgtcgtgta taattcttgt tctgcattga
     3901 cataataact atcgagttct tcaaaaatta ttgaaatacc actttctgtt tcggcttttt
     3961 tctttgcatt atgaatagtc tggacagttt tttgagcttc actgtaactt gaagaaaatg
     4021 tttttattaa atttaatact cgttcaggga taaacgtagc aatgctgtcg tttgtcataa
     4081 ttgtctcaaa attgtctaac aacaattgat cttgtaggcg gtctttttca gtgtctatat
     4141 tgtgtctatt agtgtctaat atattattat taagttctgg tatgattttc ataccctctg
     4201 atttatcaac gttttggcac ggtatgattt tcataccctc ttgagcccca agggattcag
     4261 cgtctgattt ttcgtcgagg gtgggtatga ttttcatacc ctctgattta tcaacgtttt
     4321 ccctattctc tctatcaaat ttttcgagta agtagatatc attttctgag acttttaatt
     4381 ctgctagata tagtctatta ggattatttt tccctaactg tttgttaaat cccatctgct
     4441 tttgtaataa taaaccaaag gattccaact cttttttgat tgctatcgct ttttgttttt
     4501 ggcagtttaa tacgctacat agttcatttg tcgtataagt aaaataaaca ttaccttctt
     4561 catcgatttg ccgtttgctg attgcgatct ctaatcttgc ttttaaaagc atataggcaa
     4621 tcttagttga atctttcatt tttttgtatt cgtcagaaac aataaagact tttggaaatt
     4681 gataaaataa ttcgttgtaa actctatccg ccttaaaaaa attaaaatca gtcattgtag
     4741 atcctctact ttcacattgt tttatttatg taaaattacc tactcttttc gaaaatagca
     4801 aagataaata aaaaatgcaa acgcattaaa actgtttttt aattaaatac tacctaaaat
     4861 cagaaataaa atgaattatg tactaccttt aaagtcatta agaactactt taaataaata
     4921 ttatttataa ttaaatacta ccagaagaac gtatgaataa aatttagttt taaaattaaa
     4981 gataattaag tactaccagt gtcaaaacaa aaaataaatt aagaactacc ttatgcatat
     5041 aaaacatact accattacta gtaatatata ctaccttatt tcagtagtat atattatatt
     5101 cttcttataa gacaaggctt taggctagaa aacaatagat tatatactac taaaaaatac
     5161 aataagtact accaatgtca gatatatata ataattatgt actaccatta aaattaactt
     5221 atttaataag atactactgt gttaatctga aaaaaattac tttaaagtag ttacatacta
     5281 cctgttaaat tagatactac cattaatact taaattagat actacctata tttatattaa
     5341 gtactaccgt tctaggaata agataataaa aaaaaactac caaattgcaa ttaaatacta
     5401 cttaaaatgt aaaaaataag aactacccaa attaggtagt tcttaataag agaatatcag
     5461 ataaatttgt acaagtcata aaaaaattaa tcttaaattt ttaaatatat atacctgtta
     5521 attataaact actttattta ggtagtacat aattaacaga taaaactaca tgcttgtagt
     5581 ttgtctagct aatgagttgc atatgattta tataaaggtg tgcagcataa cttagttgaa
     5641 gttaagagaa tagaataaat attttttgtt ttgctaataa ttaaacacta cctaaatatg
     5701 gtagtgttta attatataat attcattttg ttacaaataa tcgtattata aaagcataac
     5761 aattgggata actatttttt agattattta agtactactt aaattaggta gtacttaaat
     5821 tttggtttaa ggtagtaggt aattaagaaa taatatatat cttcttttat aatagaaggg
     5881 gagaggccta gttaaaagca gcgacaatta ataacaatgt atttaatgct tcacaaaatc
     5941 catttggtgg acaatgcatt gttattagcg atgatctacc gttctaagta gttttttcta
     6001 ttggttgcat tcgtgactaa ttatggtata attatgagta acaaagaggt atttcattat
     6061 atcatcaaac cccatcagcg tgtaggctga tggggttttt tttacgtttc tatattatag
     6121 aattaactgg taggcttact gtcttttttg gcaattttgc aatcaagcca cttgcagatg
     6181 tagtgtgcag ttacctctgc aaatggcttg attgcaaaaa caaaatcaaa aaggtatttc
     6241 attatatctc ctcctcccct tgccagatta ggccggggta gcagacagca aaactattat
     6301 aacataactg aaatacctaa atgacatata cagatatgat aatttatgta ttgatttttc
     6361 taacaaaagc acatatttta atatatacta cctattttag gtagtatata tttttagata
     6421 caatatcgat cagttttgag gaaatgaaat tctagatatt ttttgctaca tagagttact
     6481 ttcaatattt tttaacgtaa ttaggcagaa gtcactcatc ctctataggt ttgtgatgaa
     6541 attgctattg atccatttaa gtaagagcaa aaaactggag gtttatcagg taattggtct
     6601 aatggattta agtatgcggt caccgtgtat agagttgctt atgagattca agaaaataaa
     6661 gttattccag tattgctata tggaacacat aacaattttt atgaattagc cgagaacact
     6721 cgcaacttta ttcgtgtaga tgaatcggct tcggttgagt tataacttta gctatatcaa
     6781 tcaaccgacc gacatatgtt aaatctatat ataataaaaa tacctactta aatggatgtt
     6841 aacggaagtg tatggtgagt agttcactcg tattttgata tcgtttgtat atgttttcag
     6901 cgttctaagt ggcttatttt gtccaataat aatatggagt ggaatccctt aagaaacacc
     6961 gttggcaatt gtcgttataa catgttataa tttatttatc aaaaactaaa agaagtgtga
     7021 taacatggga aaagaactaa aggttagaaa gatcggcaat agcgtgggag tgatccttcc
     7081 aagctctctt ggattaaaaa gcggagacac tatccaagcc aaacaagaag gcaacttaat
     7141 catattagat actactcaaa tagcaaaaga acacgatcgc aaattgattg aagaaagttt
     7201 ccaagacttt gagaaaggtt taacagtttc agaaattgaa atggtaaaag ctttcggaaa
     7261 atacggctgg agtgaataga tgaaagagta cacgctagtt tattctaaaa gtttccagac
     7321 cagccttgaa acgataatag aagaatggga aaatgagctt gctctttctc cagccagcat
     7381 aaaaagattt gtaggtgcga tccagaaggc tttagaacta accaaaaatt ttccaactat
     7441 gcatgaggaa gtttccgaaa tctatggaat ggatatccca acatatcgga tcgtgatcgg
     7501 caaaagctat gccttatttt atcggatcga ccatgaaaaa aaagagatac ttgtaggacg
     7561 tattttccaa tctaaacaaa tgaaactagg cttttaacaa taattaggca aaagtcgctc
     7621 atcctctaca ggtgggtgat taatgccgct cgatatggtt gttaaccgtc ggttactcga
     7681 agattgatat ataagactta gtaccttttt attttgctcc atgttatggc cgaaggtcta
     7741 aagatacaat cattagtcta gagaaaaatt aagaaccgta ggaactacgg ggatcgcttg
     7801 gtgaataaga gacactgcta ttagtgaaaa aataagatat caagtacgct ctattcccaa
     7861 aaatttcgtg actttactca tgagaggttc aagtagtata tttttaatca aaattgacgt
     7921 aactttta
//
LOCUS       DAFEOM010000098         7779 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.103, whole genome shotgun sequence.
ACCESSION   DAFEOM010000098
VERSION     DAFEOM010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.103"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(615..1700)
                     /locus_tag="LP524_002545"
     CDS             complement(615..1700)
                     /locus_tag="LP524_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBL2235543.1"
                     /translation="MSTEIQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSQTIDKSTSSRNA
                     EIARREETAKTLDNTGNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTE
                     TDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHNAKHKA
                     EELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFIEK
                     AIAAKLANEQRETFNELPTVSTENWLE"
     gene            complement(2020..2427)
                     /locus_tag="LP524_002546"
     CDS             complement(2020..2427)
                     /locus_tag="LP524_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235544.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     KNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            complement(2446..3264)
                     /locus_tag="LP524_002547"
     CDS             complement(2446..3264)
                     /locus_tag="LP524_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBL2235545.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
     gene            complement(3490..3783)
                     /locus_tag="LP524_002548"
     CDS             complement(3490..3783)
                     /locus_tag="LP524_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HBL2235546.1"
                     /translation="MSINTFVYSHPINVYIIKYLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSMNMTDVYEKLLILENEYDQFTSNQQRKVKKQID"
     gene            complement(3919..4098)
                     /locus_tag="LP524_002549"
     CDS             complement(3919..4098)
                     /locus_tag="LP524_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235547.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLFI"
     gene            complement(4095..4445)
                     /locus_tag="LP524_002550"
     CDS             complement(4095..4445)
                     /locus_tag="LP524_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235548.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRANQNLAKEQMRTE
                     EIAEILNASVIKNRSVCIQKEERNAEGYYPDDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4438..5763)
                     /locus_tag="LP524_002551"
     CDS             complement(4438..5763)
                     /locus_tag="LP524_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBL2235549.1"
                     /translation="MLDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKNLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYQNVPDKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIKDLANWNPYTIKSRLGVIGLQLYFHANGIDRTDIAIAPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHHCKTGCVHLHIGTSILETRPGFSHQMKI
                     SITDNTRELQDYCLFLFNKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDDEK
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            complement(5872..6075)
                     /locus_tag="LP524_002552"
     CDS             complement(5872..6075)
                     /locus_tag="LP524_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBL2235550.1"
                     /translation="MKEQQLGYTLWGRPLYGLERRTAVSIDCACGELIQLNIQTKQYN
                     CKNCGNCYQFIDQDFQLLNEKNK"
     gene            complement(6235..6810)
                     /locus_tag="LP524_002553"
     CDS             complement(6235..6810)
                     /locus_tag="LP524_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL2235551.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKLFAKKNNPNFKEGRPKATITPKKRHAYELI
                     TSGKSYKEVEAITGFSRSTLFRIKKQIEASE"
     gene            complement(6826..7431)
                     /locus_tag="LP524_002554"
     CDS             complement(6826..7431)
                     /locus_tag="LP524_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBL2235552.1"
                     /translation="MILENKLGLTNQVELAKAEEKLSKQKAKELYDSGKINEIEVGTF
                     KGLSEIHDFLFSEIYDFAGKIRTVNIAKGNFRFAPVMYLEQSLNHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT         2300 a         1430 c         1151 g         2898 t
ORIGIN      
        1 accttaacac agccaaaaaa agtggtatca cttttgaata tctgttatac taaatccgtt
       61 gcgggtaata ccgtaacact gaagggcacg cattaaggag tgagcttaat gctttcctgt
      121 agggcactga cggagattac cagtctcccg agtcttacag gggcctttct tttttttgta
      181 ttttcaattg tccaaattta tcgtccaaaa gtgcagaacg aaaatcaatt catcacaagc
      241 atagcagaca attaaattta aaacaactaa aaatatacaa aaaagtgtaa ttgaatatta
      301 gcagcactgc ctgaaaagaa gtaatatcag gcttttcagc ttggggaaat aagtgaatta
      361 cctctcttca tgaattatta taactcaaaa aagcacatat gcaaacacta gttccctatt
      421 tttttgaatg aaaaataact aaaatatcta acgattatga cattaaaacc tacgtttgtg
      481 attgttttca taaactttta atctaaaaac tccggaataa taactattta tttgtaacat
      541 ttgtaataaa attaaaatta taaaaaagtt ctaatttaaa attgatcatt tttatagtga
      601 tcgcttttta tcaactactc tagccaattt tccgtactaa ctgttggtaa ttcgttaaat
      661 gtttctcttt gttcgtttgc aagctttgcc gcaatagctt tttcaataaa ccagtttttt
      721 acatacacaa aaatcaaatt ctgcaaatta tttactttct cagtcttttg tttttgataa
      781 gctttcagca acgtcatata taatccttta tccgcatcaa ttccgtaaga ttcaagttct
      841 tcatacacaa ctaccgtctt ctcaagttcc tcggctttat gttttgcgtt gtgtattgtg
      901 gtgacggttt gttgggcttc ttcgtaagtg ttagaaaatg tttttatcaa gcttaatacc
      961 ttttctggta caaatgtgtt tatgctgtct tctttcatga tttcaacgaa gttatctagc
     1021 aaaacttgat tttgtaattg gtctgtttca gtgtctttta agtgtctttt agtgtctaaa
     1081 gatttattaa tataataatc ttgtgcgatt tccgcatttc cagtgttatc aagggtttca
     1141 gctgtttttt ctcgacgtgc gatttccgca tttccagtat tatcaagggt tttagcagtt
     1201 tcctctcgac gtgcgatttc cgcattccga gaagaggttg atttatcaat agtttggctt
     1261 aaatcgtcat cgaaagaatt ctgaataaaa agatcatcgg cacttatttc aggttgaagt
     1321 aaatagtatt tatttttttc attttttttc gtttttgggt taaattctcc ttttacgact
     1381 gaaatcaaat tatatgtttc taactcgttt aaaatggttt ttactttgcc ttggtagcaa
     1441 tttaatttgt ccattaggtc tgcttgtgta aattccaaat ataatagacc atcatcgtcg
     1501 atccattttt catgtaaggc tctacgaaat tcatttttaa aaagcatata ggctattttt
     1561 gagctgtctt tcattttaga gtatttagaa ttctgaatta aaaaactagg aaatttataa
     1621 tatttttcaa tataaacatt cgttgcttta tatccccaat ttttaataat cattttttga
     1681 gattgaattt ctgtagacat taaatttctc cttcttcata gaaacttgta tatattcacg
     1741 attttcaaca agtgaactat accatgtatt aagggtgaat aaaacacttt cagtatttaa
     1801 ggtataagtt atatattaaa aaaataagta taagttatac tttaaataca aataatgtat
     1861 aacttatact ttaaataata attttataat gttgatttaa tcgcaaattt acacgttgtt
     1921 tattaagtta tactttaaat atttaatgta taacttatac tttaatattc atttatctag
     1981 tataacttat actttaattg aataccgcat gaaaaaaaat tattttggta atttttcaaa
     2041 ctcttcttgt atttcttctc ttatgtgttc ttcttttgct actcctagtc tatgctggat
     2101 atatgagtct gctaatgtat taacaatttt ttcgaaagaa attctccctt tttcactttg
     2161 taactccgtc atatagtctt gtaaaattga gagtttgcta tttaatgccg gagagatctt
     2221 tttaggaact gataccttgt ttcttttatc aggaattgaa actggtcggc ctcttttttt
     2281 caatgtaata ttttttgtgt tactcaatgc actattgcta aagttatatt caccgcttat
     2341 tttcacatct tggcgtttgt tattgggtaa taatggctca aattcgccat tctccatcat
     2401 tttaggaata tcaaaatctt taggcattac ttatgcaact ccttattatt tatttaaaaa
     2461 ctcttgtcta gcatctagtt ctgccaaaat atttataaat acttggtgtg ctttataatc
     2521 ccattgatca aaatttccat ttttgtattt tcttaaatgt ataccaattt tagaatacct
     2581 tctcaatctt ttttgataat gaatgagatt tttggatacc acggttccgt atttttcaat
     2641 tgcttcttct aggatttctt catcaaaatc atcatttggt tctaccatac aaggtacaat
     2701 atctataatt tgcaaatcaa gatcgtatct atcgaccatg aatttttgat aatttacata
     2761 tttgttgact ccctctaagg attcttcagc tgtttgaaat gaaattatcg aatagtctga
     2821 agctgccata gcattatcgg aaaattcgct aatggttggt ggtacatcaa taaatatttc
     2881 ttgataattg ttctttattg gaagaagtaa ttttttcaag acagtaactt ggtctaattc
     2941 attttcatgt ttattagcaa aagcggagaa tgatcgaaaa ctagtgtcac atgccagaag
     3001 gtcaagattt tcatttacct taataatact acttgctaat tctccatttg cgacaccatt
     3061 tgttatagtg gttttgatat cggttatacc gaaagttctt gctatgactt gtgttaagtt
     3121 cgcttgaggg tctaaatcaa taataagcgt ttttctattt cttatcattg cattgtcata
     3181 agcaagcatg ctgactatct tagttttacc tactcctcct ttaaaattgc caactgtaaa
     3241 tgtagtccct tgaaaagtat ccatgtaaat cctcccaatc tttaattgat aattaaagta
     3301 taacttatat atcggaaatg gtcaacatta aaagtataac ttaaataata atatctaaaa
     3361 acgtttggat atagcggttt ttaagaataa aagtataagt tataccctaa atatatatgg
     3421 tataacttat actttaatta tttaaggtat aacttatacc ttagatattg cttttcgaaa
     3481 atagctattc tagtctatct gtttctttac ttttcgctgc tgatttgaag tgaattgatc
     3541 atattcattt tctagtatta ataacttttc gtatacatcg gtcatattca tactagaagc
     3601 aagacttaga tcatatacaa atgaagcagg taatgatttt attctacgtt gtcgggttat
     3661 ccagcttgca accgttcctt gtgggtaggc atataactca caaaattgtt ctacagtgat
     3721 tcctaaatat ttgataatgt aaacattgat cgggtgcgag taaacaaatg tattgatgga
     3781 catggtgcgt aagcctactt tcttttatta ttgcgtttgc aataatagtt taacacacgc
     3841 attattctag tatctactcc tatttgtaaa aacaaaaaag gattcataga gataactcta
     3901 tagaatccgt aaaatacctt atataaataa cgggaaaata taaaacctaa aattatgata
     3961 acctgctgca gcttctacat agaaagagaa acgctcaaca ccaacctcat ttatcaaacg
     4021 ttggccatac ccccaaatct cttctgatag ctgatcaaag gttatatact ttagctcata
     4081 ttgctggata atgttcattc taatcgactc catttattat cctggtaaaa ttcgatatga
     4141 cgtatttcgt cgtagtgaat tttgtctttg ccaacgtaga ttcccagctc atcactacct
     4201 aagatctttc caacaatatc gtctggatag tatccttcag cgtttcgttc ttctttttga
     4261 atacatactg atctattttt tatgactgaa gcgttcaata tttccgcaat ctcttcagtc
     4321 ctcatttgtt cctttgctaa attttgattt gcacgttgct cattatctgc atcgatctct
     4381 tcagtatgat ccgataaata gaaacctgcc catttcaatc gtcctcgatc gttatagtca
     4441 atcattttta agcccccctg cgccgcctgc atggccacca accaacgtgc ttctagtaat
     4501 actacgagcg ccttctaaca tcgaagaagc atgtacaatt gacgtaaaac catacttctg
     4561 cctaatttta tctattattt tgtctagttt ttcgtcatca atctgttttt gtggatcact
     4621 aaacaaatcc agctgaagcg aatcagtata aactagcttg ctgtaagtga ttccgacatg
     4681 gcggacttct tgtccctcat agtatttatt aaataagaac agacaatagt cttgtagctc
     4741 tcttgtatta tctgtgatag atatcttcat ctggtgtgaa aaccctggtc tcgtttctaa
     4801 aatcgacgtt ccaatgtgta aatgaacaca acctgtttta cagtggtgtt gcctaatacg
     4861 gattgctact tgctcagcca tttcttttac gaccagttcg atttcatttc ttctagtata
     4921 atctcgtggt agcacttgtg aattgccata tgatttttcc tttgtcggtt ctggtgcgat
     4981 tgctatgtca gttcgatcaa taccatttgc atggaagtac agctgtaacc caatcacacc
     5041 taaacggctc ttaatggtgt atggattcca attagctaaa tctttgatac tcatgatccc
     5101 catttggttg agcctttttt tcattcgaga accaataccc caaaagtctg tcatttcagg
     5161 aatattccat actttatcag gaacattttg atacgtccat tcggcaataa atccttcatt
     5221 atgcttcgct tcattgtcta aggctagttt agccaaaaga ggattatcgc caacacctac
     5281 tgtagcaatc aatcctagct cttctttaat acgttcttgg atcatttgag cgagattctt
     5341 ccttcgctga cttcgtgttc cttcagtcgt aaaaagattc agtgatcggg tcacttttaa
     5401 gattgattca tcgatcgagt aaatcagtag atcttcatca gccacatatc ttctgaaaat
     5461 attatttacc tgcatattac gcttaatata aaggttcata cgtggtggaa caacatgtaa
     5521 tgttttaggg aaaggctgtg gtagatcccg tggtctgctg acattagaaa taccatattt
     5581 cttctttgca gctggtgaag aagctaaaat caaccctgat cctgtattat ccgctcgact
     5641 catgacaact aattcagtcg ttaatggatc taaatttctt tctatacatt cgacacttgc
     5701 gtaaaaactt ttcatatcga ttagaaagta gtcattgact ggctcttttg agtaatcaag
     5761 cataaagttc tccccctctt ctctcatatt atacaaacat acgttcgtgt ttgcaacgag
     5821 gatcttctct aaatagaaaa atagccagac aattgtctga ctataataaa cctatttgtt
     5881 tttttcgttt agcaattgga agtcttgatc aatgaattgg taacaattcc cacaattttt
     5941 acaattgtat tgtttcgttt gaatattgag ttgaatcaac tcaccacaag cacagtcaat
     6001 cgatacagcc gttcttcgtt ctaatccgta taatggacgt ccccaaagag tgtagcctaa
     6061 ttgttgttct ttcatttact tcgttcctcc ctaaaaatat gtctattcgt agctggtatg
     6121 acgcttcgtt cgaccaatga tacatcttaa tgagtgaacc tgaccttaga atgctcgtat
     6181 aaacaacgat ttatttgatt taaaagagat ttttatatta attttatcaa tcttctactc
     6241 actcgcttct atttgtttct taattctaaa aagtgtacta cgactgaaac ccgttatggc
     6301 ttccacttct ttatacgatt tgccggaagt aatcaattca tatgcatgcc tcttctttgg
     6361 tgtgattgtt gctttcggac gtccctcttt aaaattagga ttattctttt tcgcaaataa
     6421 tttcccttcc tgagtacgtt ctacaatcat atcacgttcc atttcagcta cactcaacaa
     6481 cgtacgtgta accattcgac ccatcgatgt attttcgatc gtccctagat tcaatacctt
     6541 aatcgtgacg ttatcatcga gtaaaggttc aatgatctct agtgcttctc ttgtattacg
     6601 ggcgaatcga tcaagtttcg taacaacgat ttcgtctcca ggcttcagac cagccaacag
     6661 ctgattaaac tttggccgat ctcgtttcgt acctgtaaat ttttcagaaa aaatattctt
     6721 cttctcaaca ccgttctcaa gtagctgttg tatctgttcc tttgtacttt gagaagcggt
     6781 tgagacacgt gcatagccat attttgtcat ttaactcacc tgcttttaca aatcatctat
     6841 cgtatactca gagtatcctt catagaagta gctgacatcg attcctttca tatataactc
     6901 acgatcgtca attttatcag tcagggcatt ggaaatgaga tagcgtattt ccaagtcatt
     6961 tattggactg cgctccattg cagaaagata gtctgcttta tcgatcaagt tccaatcgac
     7021 cactttttgc aattcttcct ttaaaatgag atccagccaa attctagtac tgcgcccgtt
     7081 tccttcgcga aaagggtgag cgatattcat ttccacgtat ttcttaataa tctcttcgaa
     7141 agaagtttgc ggcatctgat caatatgatt taaagattgt tctaggtaca tgaccggcgc
     7201 aaaacgaaaa tttccttttg caatattaac tgtccgaatt tttcctgcaa aatcataaat
     7261 ttctgaaaag agaaaatcat gaatttcaga caagccttta aacgtgccga cttcgatttc
     7321 atttattttt ccagaatcat ataattcttt tgctttttgc ttactgagtt tttcttcagc
     7381 cttggccaac tcaacctgat tcgttaaacc caatttattt tctaaaatca tggaaaatcc
     7441 ccttaacttt ttgacataag tttatgaaac actacaaatc ctattgtatc gcgaatttaa
     7501 ccatgtgtca aaaactatta agtttttgac acgtagttag ttttcaatag aagccatctg
     7561 tccttttctt tttaaatggc aaaaattttt tattttattc ggcacaaact ataaacattt
     7621 aatcgctaag aaaatcactc aattattttg agtgattttt tcagttctta ttttaaaaca
     7681 aagtgttcat aaaatcaggt gtttctttat ttttcagtag cataccctgt ttttattttg
     7741 agtattagta ataattaaaa aaaccacttg taattttat
//
LOCUS       DAFEOM010000099         7701 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.104, whole genome shotgun sequence.
ACCESSION   DAFEOM010000099
VERSION     DAFEOM010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.104"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            546..1109
                     /locus_tag="LP524_002555"
     CDS             546..1109
                     /locus_tag="LP524_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL2235553.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1388..1675)
                     /locus_tag="LP524_002556"
     CDS             complement(1388..1675)
                     /locus_tag="LP524_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBL2235554.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(1665..1994)
                     /locus_tag="LP524_002557"
     CDS             complement(1665..1994)
                     /locus_tag="LP524_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL2235555.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2299..2628
                     /locus_tag="LP524_002558"
     CDS             2299..2628
                     /locus_tag="LP524_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2235556.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(2915..3400)
                     /gene="vanZ"
                     /locus_tag="LP524_002559"
     CDS             complement(2915..3400)
                     /gene="vanZ"
                     /locus_tag="LP524_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HBL2235557.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3553..4464)
                     /locus_tag="LP524_002560"
     CDS             complement(3553..4464)
                     /locus_tag="LP524_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HBL2235558.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            complement(4892..5500)
                     /locus_tag="LP524_002561"
     CDS             complement(4892..5500)
                     /locus_tag="LP524_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HBL2235559.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(5506..6537)
                     /gene="vanA"
                     /locus_tag="LP524_002562"
     CDS             complement(5506..6537)
                     /gene="vanA"
                     /locus_tag="LP524_002562"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HBL2235560.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            complement(6530..7498)
                     /locus_tag="LP524_002563"
     CDS             complement(6530..7498)
                     /locus_tag="LP524_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HBL2235561.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
BASE COUNT         2352 a         1532 c         1282 g         2535 t
ORIGIN      
        1 agttaaaact tttttagcag attttaaagt ttcgtcaaaa ctaatatatt ctggtagctg
       61 agcattcaaa tagctttcag acttcagaag aaaattacga gcatttttat agtccatctc
      121 taataaagtt attctttttt tatccaatac attcagctca attccttata atattctatt
      181 ccttagtatc tcaacattat ttactttata aaacgaataa attttgtagt tctaattcaa
      241 atattgcatg ttatatctaa tatgggagca ttttataatt ttcattgtga taaactcgtt
      301 attaacttta ggatacacta gttaggatta aattaggtat ctagaaattt acttaagatt
      361 tttttgttta tttcgtatac acactcgtat caaaaacaat cgattatgag acaaaatgaa
      421 atgacctctt ttattaaata aaagaggtca tattttgtat cataacttgt tgcattaact
      481 atgtatctat aactcccttt tatgatacaa tcaagccaga attaaagtac gaaaggaaga
      541 aaagcatgag tggatacatt tatggttatg cccgagtgag tacacgttca caagaactaa
      601 atcgtcaatt agacttactc aatgaacaaa attgtaatga aatcctaact gagaaaatga
      661 cgggaaccaa agcggttcgt ccccaactaa atcgtttaaa agataaaatt cgtccgggag
      721 atacggtaat tgtagaaagt ttttctcgat tgggtcgtag tacaaaggat ttgattgacc
      781 ttgttaccta ttttgaagaa caccatgtta agctggtgag cttgaaagaa aacttcgata
      841 ccagcacgcc tcacggacgc ttaatgatga ctgtcttcca agcatttagt cagttcgaac
      901 gtgatttaat tgttgaacgt accaaagaag gtttgaaaag tgcacgtgcg agaggtcgta
      961 aaggtggccg tccacgagtg aaccagaaag atgttgatcg agcagtaaaa ttgtataaaa
     1021 gtcaggttta tagcgtaaaa gaaattacgg aaatgactgg tattagcaag gcaacactct
     1081 accgctactt aaaagacaat agggaataat ctattttaat ctttaggaat ctataatatt
     1141 tatgggagaa ttagagtgat ttttttatta ttactaaaaa acaatataaa ataacgcatt
     1201 ttaagccctc tttgtgcata aggatgaagc ttttaatttg aaagaggctt aaaatgcgtt
     1261 ttaatttttt ctatttttca taaatatata ggttagtggt ttaaaattat agggtattac
     1321 aactctcagc tgttaaaaat tatcttttaa agtgtcgtct atcctctcaa gttgaaacat
     1381 tgggttctta cctaatcttt tttaattgtt cataaaaatt ttcatgcgtt ccacatagta
     1441 agataggtat cactgcatta tctttaattt catatgcaac gcgatatgtc gtacctgcat
     1501 acttgaatcc aatagcccaa attcctgata aatcacccgt tttttgttct cccgaatgag
     1561 gacgaactgc tatctcatca taaattatcg ttaaaaattg ttcttttaat gttttctctt
     1621 ttaatttttt tagatacttt attacgtgtt tagaaggtag aatctcataa gccaatttct
     1681 gctgccaact cctcttttgt catcggttta gaatttgcaa cagtatcttc agcaatagat
     1741 ttaaatgcag aagttaacgc attttttcta tttctaaatt cttttaagat ctcttgtttg
     1801 ctataacctt ctgaaagaat atcagccaaa atatcttcac taaaatcaaa aaattctttt
     1861 ttaggctcta caaactcata aaggatacca ttctctacaa gcttagcttc tacttcaaca
     1921 ccattgggca catcgaattc tttagggacc gttaacataa tagaatttcc ttgttttctt
     1981 gtttttgtaa tcatattatt tcctccatta ttttacattt cgtaattact tagtaattac
     2041 attgtacgct atttttagta gaaatacaaa ataataaaac tcctttagaa caattttcaa
     2101 aggagtttta tttgattagg aaattatgta gaataataat atgtttttaa cttggaatca
     2161 aaaacgcact tgcaagtaag tttacttcaa tacatgaaat tgcttataat agtgtattgg
     2221 aattaaggta gattgacaaa agagtgatca aataggtata cttcattcaa ataatcgttt
     2281 gaatgtgagg tgagatgaat gaaaaaagtt tgccaagtga ctgtcgtaca taaagaaaaa
     2341 gtaaatggaa taaaaaataa attggatcaa aaagattttt caaacttact tgtgttaggg
     2401 aaatgtttca gcgatttttc tagaattaaa attttctatg ctttagaaac ctataaggaa
     2461 atgtgcgtct gtgatttagc agaaatactt actgctagtg tcgctacaac gtcacaccat
     2521 ttacgttttt taaaaaaaca tggaatggca aaatcacgtc aagatggaaa agtagtttat
     2581 tactctttag cgaatgaaga catcctttcc gctattcgtg ttttttaaag ttatcagaaa
     2641 atctatctat gaaatcttag tttgggggta ccgtcaggaa aatgcggatt tacaacgtta
     2701 aggaaattta ttaaagagac caattcctca gcactaaaat tgataattgg gctttctttg
     2761 ttactatagt gttaggtaag ctttttactt tatcaccggt tttcccctca cttcacacct
     2821 acgggcgagt ttccccacat cccgttttcc atctaatcca attcattcaa tatctttatg
     2881 tgtatgaaag atcagattgc ttgatttaga catcttacac gtaatttatt cttaaatggg
     2941 tacggtaaac gagcaataat acgagcaaaa gtatacctac aaaaataata actctgtcta
     3001 attttttttg attcatatgc ttattgctta aaccatataa tttcagtcca agaaagcctc
     3061 caacagtatt tgtaattaca tctgttatgt ctgtcgctcc aatagcgaag ataaattgaa
     3121 ttatttcaaa agtaagactt aaaaccagta caaaagcaaa cttaggtaaa aatccgattt
     3181 ctttaaaatt gacattcaaa agcaagccaa atggaataaa gattataaca ttatctatca
     3241 tctctctgaa attcccagta gcagtaaatg gagtcaagtt aagacttctt tgatgataat
     3301 taaatactga taaaatattg tattgtaatt tgaataacac taaccagatt agtgtcacta
     3361 aatataaagc tagcaatcct ctagataata tttttcccaa tataatttcc tctaattctt
     3421 ttgtttaagt tgtatcatta gtatagcaat gtcctagtta acgtgggttt taaattaaaa
     3481 agctaacgtt accccaaaag tgctagtaca ataatgctgt tcattaattt cgttggtttc
     3541 attccgccat ccttacctcc ttgaattagt atgtgttgat ccgggaaaca ctgtcacaat
     3601 tacaccgtct atattgtttc ctgatatctc ataacgaaga ttagtcggca catgaatggt
     3661 ggtattttta gtaacaggat aataaaagat ctcatatttt tccccattta cactaacaga
     3721 aatggttttt tcttctttta ggtaatccat atattcctcg agaacgaaat tcttttcttt
     3781 cataatcgca ctatgtggta aaccaacata gcgaatatgc catggttcat attgaattcc
     3841 tgttaactct gttttgtcct ctggataacg taaaatgaac ccgtatttcc aagcattttc
     3901 ttctatccac tttccttcag gggctcgttc cattttcgtc aagcttgatc ctacatctag
     3961 tgataaacct gaattatgct cactataacc tgctggtaag gcatactcag cccccatttc
     4021 ttggtaaagc acactttgct catcaaagtc tcgatagcca ctattaataa taaaatgact
     4081 aacgccaccc tttacagcat cattgaccat ctctgaaaat ttttgtgcta tttcttttga
     4141 catataaata ttactatcaa gcaacccgta tccatttatt aattcgtcat gtttagataa
     4201 attcacgata tctgacttca cactttcttg gcgaacagga tatttactat tgattaatag
     4261 cagatttcct tgataaacct gttcttctgt aattgttttc tcttgggtat tttcagaagt
     4321 cccactattt tctaaatgtt ctttgggatt ttgatcataa ttttgaaatt cgactttttc
     4381 ctgagaaaac agtgcttcat taacgtagtc ataacctaag tatattaaga ataacaataa
     4441 caataaaaaa aacaacttct tcattttcag tctcctcctt aactatatag ttggaagtat
     4501 gaaatatttc tagaatcttc cccttaaacg gcacaatctc gtttgagatt gtgccgtttt
     4561 gccccgtaaa ttacagtcaa ttatttcgcc ctactggatt tactattatc accaatgtag
     4621 atgaattttc ataagtgata cccatgctga atttaacgag gctgaactgc ctgttgacaa
     4681 tgggcggtgt gaaataccac ccctctatgc gatcagtctt attatcaggc tatcttgccg
     4741 cagcgcacgc agataatgac cgcaatatcg ctctgcctta catcttcaat actatctact
     4801 atatataaat tccctaagaa ccagttacat acgtcgggtt tcctgccgaa agagttagct
     4861 tatatagttt gtccgtgcaa cggttaaaag tttatttaac ggggaaatca aaatagctat
     4921 tggggtatgg ttcgtctctt aatacatagt gccaccattc gaggctatat gcttcaaacc
     4981 cactgttttc catgatggag cgcaaacgtc tgcgattttg cgcttcattg catgatattc
     5041 catttgccgc atgatgagag cgttcatcca taaaatcaaa tcggctcccc attggtacaa
     5101 gctcacccgt gtctaatcga taaagcgtaa gatcaatggc actgccgcgg ctatggcttg
     5161 attttgaagc cacgtatcct tttgaaatca tctcagttcg gtcaatattg ggataataac
     5221 tttcctttgt caggttattt tccggctgtg cagcccattg cataaaacag tttacagcac
     5281 gattaggacg gtaaccgtcc catagaagca atccgtaccc ttgggtagca gccagttctt
     5341 ttgccttcaa aagcgattca gccaactcgt atgtccctac aatgcgattt acttcataac
     5401 cgtcaaccgg ttttccggtg aaattatccc aagtggcata tttagcgtcc caacgaacac
     5461 cgtgtactat ttcatctaaa aaagtaaatc ctatttccat gcttatcacc cctttaacgc
     5521 taatacgatc aagcggtcaa tcagttcggg aagtgcaata cctgcagcgg ccatcatacg
     5581 gggataacga ctgtatgacg tgaaaccggg cagagtattg acttcgttca gtacaatgcg
     5641 gccgttatct tgtaaaaaca tatccacacg ggctagacct ctacagccga gcgctttata
     5701 tatttttttt gccgtttcct gtatccgtcc tcgctcctct gctgaaaggt ctgcgggaac
     5761 ggttataact gcgttttcag agcctttttc cggctcgact tcctgatgaa tacgaaagat
     5821 tccgtactgc agcctgattt ggtccacctc gccaacagct aacgcggcac tgtttcccaa
     5881 taccgcacaa ccgacctcac agcccgaaac agcctgctca attaagattt tgctgtcata
     5941 ttgtcttgcc gattcaattg cgtagtccaa ttcgtccgcg ctattgactt ttttcacacc
     6001 gaaggatgag cctgaacgcg ccggcttaac aaaaacagga taggtaaacg tagctgccac
     6061 cggcctatca tctttattaa taacccaaaa ggcgggagta gctatcccag catttttcgc
     6121 aacgatgtat gtcaacgatt tgtccataca aattgctgag ctttgaatat cgcagcctac
     6181 aaaagggata ccggacaatt caaacagacc ttgtatggat ccatcttcac ctgacttgcc
     6241 atgcaaagct gaaaatgcta catcaacatg gttgatttca tattcatggt tctttttaac
     6301 aagtaatccg tgcatttttt tatccggcga gagtacagct gaatagcaat tgtcgttttc
     6361 ccattccgcg caaggttttt cgcacatttt ccatacacca gatttcgtaa ttccaatgta
     6421 taacggctcg tatttttctt tattaatgtt agcggctatc tctattgcag attttaccga
     6481 tacgtcatgc tcctctgagc aacccccaaa cagtattgca acttttattc tattcatgct
     6541 cctgtctcct ttcaaaatcc aaacagtttt taatggtttt ttcaacggta tcacgcaacg
     6601 cttgctcggt ataataggcc gtatgcggtg tgattatcac gttaggcatt ctttgaagtt
     6661 taagtaaaaa ttgattatca attggttttt gggtgcaatc agagtagaaa aactcttcct
     6721 ctccttccaa tacatccaat gcggcaccgc ccagtttccc gttttctaat gctttaacca
     6781 actcataggt atctacaagt ggaccgcgcc cagtattgat aagaaatgct ccttgcttca
     6841 ttctctgtat ttgttcgtgg ctgataatat agtgcgtatc cgtattgagc ggcacatgaa
     6901 gcgtaacgat atcgctattt tgcagcaact catcaaacgg tacatagttt acctctatac
     6961 ttcggctgcg actataagcc aacactttac atccaaatcc tcgcagccgc tcaataaccg
     7021 ctttgcctat ctggcccgtt cccaccacac caactgtcat gtcgctgagt accttgccac
     7081 ggtcgctgtc caacctgaaa tcatgttttt ccacagagcg cacaatcgat tttacgttgc
     7141 gtactgccat aagaattagc atcatagtat aatcggcaac gctatccggc gagtacgcca
     7201 cattgtcgac agtgatgccc attctcttag cagcagttgt atctatatga ttgcagccga
     7261 tgcttcgggt agaaatatat ttcacaccgg ctctcttcag cgcaagaaga atagaggcgg
     7321 aaatctctga tttatgtccc acactgatac attgattgaa aggcgcggat ttggcgttgg
     7381 attccgacac gttggcgtta attatcgttg ccataacgcc aaagcgaggc gaaagagcat
     7441 ggaatgcatc tgcctcatcc tgctcacatc cataaacagt aatgccgatg ttattcataa
     7501 ttaagaccaa ccctttctgt gaaaggcacc aaataaaacg gggatgccaa tggtaatctt
     7561 gtcaaatctt tctctgaaga acgaaaacgg ctcgttctta cggtattaag tctaccgtgt
     7621 aagaacgagc cgtttcttaa tattaaattg ttaatttcct aagaaaaagt aaagataacc
     7681 ttgagatttt cctaaaaaat t
//
LOCUS       DAFEOM010000100         7644 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.105, whole genome shotgun sequence.
ACCESSION   DAFEOM010000100
VERSION     DAFEOM010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.105"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            307..1395
                     /locus_tag="LP524_002564"
     CDS             307..1395
                     /locus_tag="LP524_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBL2235562.1"
                     /translation="MKEERKLPEWLIDYAEKEGLIAELKPKHERQNFLVGDDRLDHAV
                     AFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFNLKIGDPKHLKF
                     FESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRRKQTFPQSIEIC
                     VDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKATYEAVAKEMSVE
                     PEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKEACTTVAKELKV
                     CERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEFVPEVKKDWNDQ
                     LKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            1406..3415
                     /gene="topB"
                     /locus_tag="LP524_002565"
     CDS             1406..3415
                     /gene="topB"
                     /locus_tag="LP524_002565"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBL2235563.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKRSEGFI"
     gene            3531..3680
                     /locus_tag="LP524_002566"
     CDS             3531..3680
                     /locus_tag="LP524_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235564.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            3761..4357
                     /locus_tag="LP524_002567"
     CDS             3761..4357
                     /locus_tag="LP524_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBL2235565.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            4370..5269
                     /locus_tag="LP524_002568"
     CDS             4370..5269
                     /locus_tag="LP524_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBL2235566.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEDIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            5272..5403
                     /gene="ssb"
                     /locus_tag="LP524_002569"
     CDS             5272..5403
                     /gene="ssb"
                     /locus_tag="LP524_002569"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL2235567.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            5425..5757
                     /gene="ssb"
                     /locus_tag="LP524_002570"
     CDS             5425..5757
                     /gene="ssb"
                     /locus_tag="LP524_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL2235568.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            5802..6035
                     /locus_tag="LP524_002571"
     CDS             5802..6035
                     /locus_tag="LP524_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235569.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(6194..6316)
                     /locus_tag="LP524_002572"
     CDS             complement(6194..6316)
                     /locus_tag="LP524_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HBL2235570.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            6506..6730
                     /locus_tag="LP524_002573"
     CDS             6506..6730
                     /locus_tag="LP524_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235571.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
     gene            7173..7379
                     /locus_tag="LP524_002574"
     CDS             7173..7379
                     /locus_tag="LP524_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBL2235572.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            7379..7630
                     /locus_tag="LP524_002575"
     CDS             7379..7630
                     /locus_tag="LP524_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235573.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEVLLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
BASE COUNT         2759 a         1335 c         1605 g         1945 t
ORIGIN      
        1 atggctgtag tcaataaagg acgagactat cgcttagaag accatgattc atttgttttt
       61 gatcgaagaa aacaacgttt ttattggaac agtcaaaata ttagtggcga cattattgaa
      121 ttagctaaac tttttttcat agacaaagag atccaagacc ctaagcaaca atttaaagca
      181 gccactgatt ttattctaaa gaatgaagac aaaacagaac gagtggaaaa tcttcatttt
      241 gagacagaaa agtacaaaga tcaccccgtt gattatcaac cactcacaga aaaaggaagg
      301 aattatctga aggaagaacg aaaactacca gagtggctga ttgattatgc agaaaaagaa
      361 gggttgattg ctgaactaaa gccgaaacat gaacggcaaa attttttagt tggagacgac
      421 cgtttagatc atgcggttgc ttttttatgg aaagatccac agacaggaga gacagtaggg
      481 gcaagttatc aaggaacgat cgtcgatttt aatcgtttcg gtaaacgggg aacgtataaa
      541 cacattgaca agaatcctac gccaaatcac ggatttaatt taaaaatagg cgatccgaag
      601 catttaaaat tctttgaaag tagtattgat ctgttaagtt acgctgcatt gaatcgggag
      661 aaactacaag atgcctggtt agtttcgatg gacgggttaa aacatcatgt aatcagtcat
      721 tacgttgaag agtcgatttc tgaattaaga cgaaaacaaa catttcctca atcgattgaa
      781 atatgtgtgg acaatgatcg tgcaggacat attttttatg aaaaagaaca gatgaaggga
      841 atcgttgatc cttttaccaa taagaaaatc cgttgtgagc gaggaattcc aaatgattgg
      901 caagtaccta aagaatacaa agctacttat gaagcagtcg caaaagaaat gagtgttgaa
      961 cctgaagcaa ttatggccat acacaaaact gaaactaatt tacagctgac aaatcaattg
     1021 gtgtctgctc atgatgttca atctacgttt ggaaagatgt tggcgaaggg cgaaccagta
     1081 gaaacaattg atttgaaaga agcatgtacc acggtcgcga aggaactgaa agtatgtgaa
     1141 cgcgcagatg gaacatacaa ctttgatcgt ttttatagta gaaaagccaa cataaaagac
     1201 gttaatgcag gaatccttct ttcctataaa gcagaacaat actacaaagg ctataaaaag
     1261 catgaacatg aatttgtacc tgaagtgaaa aaggattgga atgatcaatt gaagcatgag
     1321 atccagcaac aagaaatcag aaagcaaaaa cgtgcgatgt tgtttcaaca aggcagacaa
     1381 caagaaaggg agtgaagtcg tcactatgaa aaccgtcatt ttagcagaaa aaccgtcaca
     1441 agcgaaggct tatgccgata gcttttctaa agctacccga aaagatgggt attttgagat
     1501 ccaagatcga ttgtttacag gagaaactgt gatcacgtat ggttttggtc atttagtcga
     1561 gttagattcg cccgatatgt acgatgaaaa atggaaacaa tgggcattag aacatttgcc
     1621 tattttcccc aatcaatacc actatcatgt tcctaaagat aagaaaaagc agtttagtgt
     1681 cgtcaaacga cagctgcagt cagctgatac gattgtgatc gcgactgata gcgatcgtga
     1741 aggggagctg attgcctggt caatcattca acaagcaggc gccaatcaag gaaaaacctt
     1801 taaaagactt tggatcaact ccttagaaaa agaagccatt tatcagggat tccaacagtt
     1861 acgagacgca gaagagacct atccaaaatt tgaagaagta caagcacgtc agatcgccga
     1921 ttggttaatt gggatgaacg gtagcccact gtatagttta ctcttgcagc aaaaaggcat
     1981 accaggaagt ttttctttag gtagagtcca aaccccaaca ctctatatga tttatcaatt
     2041 gcaggaaaag attagaaact tccaaaaaga accatatttt gaaggaaaag cccaagtaat
     2101 cgctcaaaat ggcgcatttg atgcgaagct tgatccaaat gaaacccaag cgacacaaga
     2161 agcatttgaa gcctatttaa aagaaaaagg tgttcaatta ggaaaacagc caggtacgat
     2221 ccttcaagtc gaaacagaga aaaaaagtgc ggctagccca cgtctttttt ctttatcgag
     2281 ccttcagtct aaaatgaatc aactcatgaa agctagtgcc aaagatactt tggaagccat
     2341 gcaagggttg tatgaaggaa aatatctaag ctatccgcga acggataccc cttatattac
     2401 ggaaggggaa tatgcgtatc tgttggatca cttagacgaa tacaagcatt ttttaaaagc
     2461 cgaagcgatt ccgactccta ttcatacacc gaatagtcgg tatgtgaata ataagaaagt
     2521 ccaggaacat tacgcgatca ttcctacaaa aacggttatg acagcagcgg cttttgaaca
     2581 actaagtcca ctgcagcagg ctatttatga acaagtgtta aaaacaacgg tggccatgtt
     2641 tgcggaaaag tatacctatg aagaaacaac gatcttgacg caagttcagc agcttcagtt
     2701 aaaggcaatc gaaaaggtgc cgctagatct aggttggaag aagttatttg gaaaagagag
     2761 cgacggaaaa gagaaggaag aagagccgct acttcctaaa gtgacaaaag gagaaatggt
     2821 gacggtggat ctacaagttc ttgaaaaaga aaccaagcca ccacaacctt ataccgaagg
     2881 tacgttgatc actgcaatga aaacagctgg gaaaaccgtg gatagtgaag aagcgcaatc
     2941 catattgaaa gaagtggaag gaatcggtac agaagcgaca cgggcgaata ttattgaaac
     3001 cttgaaacaa aaggaatata tcaaggtaga gaaaaataag ctcgtggtga cgaataaagg
     3061 gattttgctt tgccaagccg tggaaaaaga gccgctgctt acgagcgccg aaatgaccgc
     3121 caaatgggaa agctacttgt taaagatcgg tgaacgaaaa ggaacacaaa ccacttttct
     3181 agccaatatc caaaaatttg tctctcactt attggaagtc gtaccagggc aaatccaatc
     3241 cactgatttt ggatcgacgt tacaagaagt gaaagcagcc agtgaaaagc aagaagccgc
     3301 gcgtcattta ggaatttgtc caaaatgcca ggaacaagaa gtcttactct atcaaaaggt
     3361 ggcagcttgt acgtctgaag cctgtgattt taaaaggtca gaagggttca tttgaagcaa
     3421 cgattgtttt aaaagaagac ttcactacag gctttgaatt ttctgaaaag aaaaaaacga
     3481 acttcaagaa aagaacacgc cgcactgcaa aataatggag ggttaatctt atgcgtaata
     3541 ccacacacaa acaacaagaa atcgaaaatc aaaaagccaa aattcgttca gaacaggaaa
     3601 aaatgcgtgc cagcaaacga agactgcagc aattgaagaa ccagcagagg attcaaaaag
     3661 aaatcgaatt agatcgataa aatcttccta actattgtat acgtataaac ttatgttata
     3721 ctgtttttat aagtttatac gtatacaaag gagggagaaa atgacaaaag caatggtata
     3781 caagctgcta gaagaattta atggaacttt ggctttgaag gatgccaaaa aagcgggcat
     3841 ttctcctgta acgatcaaac gtatggtaga tcgaggggag ctggatcgag agtatccagg
     3901 ttttttcacc ttacctggtc aatttcctga tgaattgttt atggcgcaaa aaaaatatga
     3961 acgcggcatt atttcacata ttaccgcgtt agatttgtat gatttaacag atatgatccc
     4021 tagacagatc gatctgactg taccttacgg ttatcacgtt tctgaaaaag gattgaagga
     4081 attcgcagtt gagctacact atacaaaagc ggaatggtat gaattaggta agattgaaat
     4141 aaaaagtcgc tatgggaatc ctattatagc ttatgatcca gaaagaactt tatgtgacat
     4201 ttggaatccc tggtataccg ttgaggatga aatcaaagta aaagcaatca aaaactatat
     4261 ggagtcggac agaaaaaatc ttagaaaatt gaatgagtat cgacgaattt taccaacaga
     4321 caaaacgatg cgttcttata ttatggcttt aaattaaagg agaagataca tgacacctac
     4381 gcagttaaaa gcaaaagtga agaatatttc tagagaaaaa gaagttgatc cgcagcttgt
     4441 tttaagacat tttatgatgg aaaaattctt ggaaaaaatc tcagagtctc cttatcgtga
     4501 aaattttgtg ttaaaaggcg gcttcttgat tggctcaaag tatggcattg aaaatcgcac
     4561 aaccaaagat attgatacga ctttacggga aatgaaagta accaaagaaa cattaacaac
     4621 ggttctaaac gaaatttttt caacgccgac gaaagaagac attcaatttg agattcaagg
     4681 aatgaaagaa acgagagaag ccgattacta tccaggattt agcttacgtg tgctggcaca
     4741 tttggaaaac atgcggccag attttaaggt agatgtgact acaggtgact cgatctatcc
     4801 tgcaacgatc actcacagtc ataaattgat gtttgaggat cgaacgattc ctttggaatc
     4861 ctatcctaca gaacaaatta ttgctgaaaa attaagtgca acctttgatt ttttaacaga
     4921 taattcgaga ggaaaagatt tttatgatct ctatactatt cctaaaatgg agaagatcga
     4981 tgagaagacc ttgaaagata gtgtgagaaa tacatttaca cgtcgaggaa aaactgatcc
     5041 gttaaagata tactatgaaa aggatatgga tgtactgaaa aatagtcacg agctaaaaga
     5101 tacctggaag aaatatcaaa aaagcaatcc ttatgctgcg tcgatcacgt acgatgagac
     5161 aatggattcg attagtaatt tgatggaaaa agtgattcac gtcgaagaaa aagagcgaaa
     5221 actagcttat caaagacacc aacagatgaa tagagaaatg gagagataaa gatgattaat
     5281 aatgtgacat tagtaggacg cttaaccaaa gatcctgatt tacgttatac cgcaagtggt
     5341 actgcagtag cgacttttac cctagccgtc aaccgaaatt tcacgaatca aaacggcaat
     5401 tgagaagccg attttattaa ctgcgtgatt tggcgcaaac cagcagaaac aatggcgaca
     5461 ttagctaaaa aaggtagctt gattggtgta gttgggcgga ttcaaacgcg gacttacgac
     5521 aaccagcaag gacaacgtgt atatgtgact gaagttgtag cagataactt tcaactatta
     5581 gaaagtaaag cagcgacaga gagccgtgcg cacgctgatc aatcgtccac cagtccaagc
     5641 accgccacct ttgaacaacg gaatacagcg acgcctaaca acaatggatt gaacgcttca
     5701 cagaatccat ttggtggaca atcaatcgat attagcgatg atgatctacc gttttagttt
     5761 gagaaagaag gagtaagcat ggcaaaaaaa ttagtctgac gatggatcta ttgatacccg
     5821 taattgcatt tatagacggg atttctttgg gcgtcactga aaatcctttg gtaaatagaa
     5881 ttgcgaaagg atcactattt cttgtattcg tgtatcttgt ctgttcttat cttttaaaaa
     5941 gagcaggaaa ggataattcc ttttggcgat tgactaaagt cacagtagca gcgacagctg
     6001 ttatacttac ggctattcta ccgctttata tgtagagtta acgggaaatc gttgaacttc
     6061 ttgcgaaata ttgctataat gaacatgaaa aagaggagtt gcgctaacaa cccctcatta
     6121 acagcaccga ttaaagaacg gtggcagaat attagttatt tggaaataac cgctgacctg
     6181 ctaaagtaga cggttatttc tttttgtcat ttttaagcat atttacaatc aaactaatca
     6241 aagcaatggt aaacatacca aaaccaagaa tggtttgaat tgtctcatat gcggacaaaa
     6301 ggcttactcc tttcatagat tttagcactt acattcataa gcaccaccac ctttccagaa
     6361 gtcgccaccg tcttcaaact ttactgtctt gactattata gcatatctaa aatcgaataa
     6421 aaattctcaa ttttagctgt ttttgctata attttagcga ggtgatcgtg atggaacaat
     6481 atattggttt actcgataaa ttagggtgat caagacacag ccattgcttg tgcgatttac
     6541 attacgcacg agtcatgaac caatcaattg tgtagtggcc aatatagaaa tagcggataa
     6601 attgctgatt ttggaagaag gcaagtatac tatttcagtg acaggtcact tcaataaacg
     6661 aaagcaacta gtcattgaat caatgcaaat tagaaaccct gatcatttta cgcgatcgat
     6721 gagcatataa aagaagagct gcagtcctaa atgaggattg tagctctttt tgttttacat
     6781 agaaacagta aaaatgaaaa ggcctgttta ggaaggcaca agcgccttct caattgaaag
     6841 acctttctct cactccaacc gccaccgccg ttcgagaaga aaggaagtga acgctacgcg
     6901 ttcactaaga taaagggtct tgcatagcaa gacaaaatag aaaaaatttc ctctcaccaa
     6961 aaggttcgct cctgttcgtt atctctttat tgatttgtca gaccggcaac aagttgcctt
     7021 ttatcaccaa aaagcgacaa ggacaagggt aaatggcacg ctgcgcgccc ttgtcttgtc
     7081 gcttttcgtt gattcattct ggccaacaaa tcaataaaga aagaactaac gaaaggaagt
     7141 aaaacctttc ctaagaggag gcagaatcaa tcatgcgaaa gaaatttgat tattggggtg
     7201 ttcccttcag cgaattattc ccaaactatc atgcaccaca tactgtcgaa tgcgattgtg
     7261 gagaaagagc gaaatgtatc aaaagctatt gtttgtatca atgtccaaca tgcgggaaaa
     7321 aatatacctt gtcttatggc gattatattt taattgatga gaaaggaaag aaacgctgat
     7381 gaaaacacaa cacaaattat tgactgtaaa agaagtcgtt caaattttgg tggacaaaga
     7441 agtcatttct gaaaaagtag cagaagtttt acttattccg cggtactgga cgatagaaac
     7501 ccctttagta gaggatgcac cgactatgct atttgaagac ctagtgaaga ttgcaggata
     7561 tgaactgcat tatcaagaaa acttggaaac ttatgaaaac gaatattggt taaccaaatt
     7621 agagaactaa aaaggagaaa aaaa
//
LOCUS       DAFEOM010000101         7600 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.106, whole genome shotgun sequence.
ACCESSION   DAFEOM010000101
VERSION     DAFEOM010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.106"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            24..1229
                     /locus_tag="LP524_002576"
     CDS             24..1229
                     /locus_tag="LP524_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBL2235574.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMNF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAMKHWGEGGNDNGTITGEWTNPFPGSSLDKSTFSGGQLFGTNPGS
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPFLNSSKK"
     gene            1245..1889
                     /locus_tag="LP524_002577"
     CDS             1245..1889
                     /locus_tag="LP524_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235575.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQATNTYKIEEVAK
                     NASEKLKKNSSSAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVNEAQSKTAEEFG
                     KLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            1900..2706
                     /locus_tag="LP524_002578"
     CDS             1900..2706
                     /locus_tag="LP524_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235576.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMIIGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFAVKLSHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVAEDQENTENKINSYQQEIETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            2729..2958
                     /locus_tag="LP524_002579"
                     /pseudo
     CDS             2729..2958
                     /locus_tag="LP524_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2979..4052
                     /locus_tag="LP524_002580"
     CDS             2979..4052
                     /locus_tag="LP524_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HBL2235577.1"
                     /translation="MKKTYQKFLWISYIVLFIVGFLVSVSVIVGTLFLLFSDEDLVLK
                     GLMSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIEYTWLPLKRNSKR
                     KEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQIAASNIFTAVMTL
                     MCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDRFEQSEKTVEGIK
                     NFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDLIPTLSGYEYYVD
                     FGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYNFYKVEASLRYGI
                     GKLRLDGSNIQQNKPYVLGKVVI"
     gene            4096..4365
                     /locus_tag="LP524_002581"
     CDS             4096..4365
                     /locus_tag="LP524_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235578.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            4597..4893
                     /locus_tag="LP524_002582"
     CDS             4597..4893
                     /locus_tag="LP524_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235579.1"
                     /translation="MNVKIRDLDPKFISEIDRRCVELSNRTGNKWSRNDYLKLLIEND
                     FDRPLMEYKKEKFDQLLEKFSEIQSYNTKILEEYVSQNNRILEILIEQNRGSEL"
     gene            4895..5317
                     /locus_tag="LP524_002583"
     CDS             4895..5317
                     /locus_tag="LP524_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235580.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDEIISTHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKSNQTKKYSPQLKKPVNESVHARVDPFDPTKILKR"
     gene            5336..6928
                     /locus_tag="LP524_002584"
     CDS             5336..6928
                     /locus_tag="LP524_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235581.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPDE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMGNGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIEGVD
                     QMQTIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            6949..7284
                     /locus_tag="LP524_002585"
     CDS             6949..7284
                     /locus_tag="LP524_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235582.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVNGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            7430..>7600
                     /locus_tag="LP524_002586"
     CDS             7430..>7600
                     /locus_tag="LP524_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235583.1"
                     /translation="MVHSTIGVVVEKSRDNLVFVTEIQTGRSFVVTDKAAKAYQSSDI
                     LTLNLTTKTFVDA"
BASE COUNT         2859 a         1130 c         1575 g         2036 t
ORIGIN      
        1 aaattgtttg atggaggaaa ataatggaaa accaaaatga aagtttaatc aagcagtatg
       61 ttaagcgtcg tgcaaagcga cgcttatttt tgtggttgtt tggtacttct gcaggtttga
      121 ttactatttt gatcactgtg ttcgtgaccc tttttctaat actggcggcg ggttcgattg
      181 ataattctga ttcagattca agcagtggtg gcgaagcttt tacaggagaa tattcggaag
      241 gattacccat ttataaagaa atcaaaggaa gaggcccttt ttctgatgag attgcgcaat
      301 atgctgtagg tgcagcagtg aaatataaat tattaccaag tgtgatcctt tcgcaatacg
      361 gttacgaatc agcgtttggt acgtcggcgt ccgcaagaaa tgatctcaac tattttggga
      421 ttacgtggtt cgatggctgt ttgtttccta aaggaactgc acgaggaatt ggtggtattg
      481 aaggtggctg gtacatgaat tttcccaact caaaagctgc attctcctat tacggcttta
      541 tggtagccac acaatcaaat tttaatgcat gtgtaggaaa taaaagccct ggcgcaagct
      601 tattgatctt aggacgaggt ggttatgccg ctgcaggaat tactgaagat agtccgtatt
      661 atacaggttg tatgtcaatt atcacttcta ataaattaac cgaatatgat gaattcgcga
      721 tgaaacattg gggtgaaggt ggtaatgata atggcacaat tactggtgaa tggacgaatc
      781 cattccctgg cagcagttta gataaaagta cctttagcgg tggtcaatta tttggaacaa
      841 atccaggcag tgaattccgt ccgaatggct tccatgatgg attagatttt ggttcagttg
      901 atcatccagg aagtgaaatt cacgcggtgc atggaggaaa agtcgtgtac gttggcaatc
      961 ctggtatttc aggtttaggt gcttgtgtga tcgtgattaa ttacgacggg ttgaatatgg
     1021 tatatcaaga atttgcgaat agtacaggaa attctcgtgt gaaggttgga gatcaggtga
     1081 aagttggtca agtaatcggg atccgagata cagcacactt gcatttaggt ttcactcgaa
     1141 tggattggcg tcaagcacaa ggacacgcat tcacagatga tggtacatgg attgatccat
     1201 taccattttt gaatagcagc aaaaaataga tcaggagaaa aaatatggaa aataaaactt
     1261 taaaaatcgt cttaacagct gcaattagcg ttagtttaat cggcaatatc gcgttgtttt
     1321 ggcagctgaa agaggaacaa gcgacaaata cctataaaat tgaagaagtg gctaaaaacg
     1381 cgtctgagaa gctcaaaaaa aattcctctt cagcgcaggc aaagatcgac aagttaaatc
     1441 aaactattaa tgagaaaaac caagaaatcg atcagctaaa gcaacaagat ggcacaaaag
     1501 caaaagaaga agtaaatgaa gcacaaagta agacggcaga agagttcggt aaattggcca
     1561 ttgataaatc attaagcaga aatgaattaa cggaaaaact aaaagatgta gcaacccaag
     1621 aagtgattga caaattaagt cctgcagacg atgatcacca acatgatgat tacggaagtt
     1681 actcctttac attaggtgac gtgcaaactt acgctcaaac aagcagtgta aaagaggaac
     1741 aaaattttgt cgtatttgtt gattatacaa tcggcaatcc tcaattcaaa gatgtcaaac
     1801 cgcaaaaaat aaaaggtgga ttaactgtca ctgaaaaaca agtagatggt caatggaaag
     1861 tcacagactt tagttatttc actcgttagg aggaagaaaa tgagtaagaa aaaaggagca
     1921 agacaacgtt tcaaaaaacg aaaaagttat cttagatggc tgggaatctt tatgattatc
     1981 ggctttttaa ttcttgctag tccgatttat acgcataaga aggtagacat accagatgag
     2041 gtaagcacga acgcattttt aaacacgaag gaactgacga tggtttcaaa agaaaaagga
     2101 gcgaaccagc aattagtttt tgaatttgcg attgacgatg aagatcaaag cttattaaaa
     2161 gaactggcta atcttaatta tcgcgtagaa gtaaagacag ctaaagggga ttatcaagca
     2221 ataaaagcca aagtgattaa ggtgtctgat gattattttg cagtgaagct atcgcatgta
     2281 ccagaagaat atattgctat gcgattaact attattcctg aaaaaattga tccaaaggtg
     2341 gatatgcaag aaccgcaaga tctgattttt tatattcacg aagataaggt caaagatcac
     2401 gtgaaagacg aagattatga acaacatgcg atcaattaca aagtgaaagg atacaagaaa
     2461 gaacaaaaag cagcacaaaa acaaattgaa tccttccaag caaccattga tttgaatgaa
     2521 gaattagtga agaaactaaa agatcaattg ccttatcaag tcgcagaaga tcaggaaaat
     2581 actgaaaata agattaatag ttaccagcaa gaaattgaaa catcgaaaac gcaaatgaaa
     2641 gatcaagaag agagcattca aaaacttcaa gaaaaaattg atcgtatgac aaaagaaaat
     2701 atttagaaga ataggtggag taaaagaaat gaagcgagcg aacttggtcg gcaaagatac
     2761 tttcgctgag cagaaagtat tgattaaagc ctatctcaaa gttgtaaaag ctaaagaaat
     2821 atgtcaagaa gtgaattatc taacacaaca atatctgagt tgaagaatgg tagaaagagt
     2881 ttagatacct taaaattcag atcatttgaa gctctatata tagctgctaa aaaatatttt
     2941 acagctgata ataaataaaa aattaaggtg ggttctatgt gaaaaaaacg tatcaaaagt
     3001 ttttatggat tagctatatc gtcctattta tcgttgggtt tctcgtcagt gtgtccgtga
     3061 tcgtcggaac gctttttctt ttgttctctg acgaagatct ggtacttaag gggttaatga
     3121 gtacgttatc gtgtgtcatg ttactttatt tatgtataca gatcaacgaa attatgcccc
     3181 ctcattggca agaaaagaag aaagaattta aggaagaaat cgaaataaaa tccaatgact
     3241 ggattgaata cacatggctt cctctgaaaa gaaatagcaa acgaaaggaa ttttgggcat
     3301 tgcaattatg gccaatagga aaacttttta tttatatggc ttcacttatg gttactttaa
     3361 tcggttattt ggtggtggtc tatggggtgg cctattactt gttaagtgag ccaaaacaga
     3421 tcgcggcgtc aaatattttc acggcagtaa tgacattaat gtgtgtcatg tgcttttcta
     3481 tttttagtta cttactcgtc caatttatca ggcaaaattt aacaatgagt aaacttttca
     3541 aaagtttatt ggacttactt atcttaggca gtatgttaac gactctaaca gatcggttcg
     3601 aacaatctga aaaaacggta gaggggataa agaacttctt aacgacgatt gatcaattta
     3661 taaatggata tatgtatttg tttgaagtag gctttattct cactttgttt ggttgtgtat
     3721 ttataaaact cattaaaaga tcaatcgaaa aagaaatggt tattgatcta attccgacgc
     3781 tgagcggcta tgagtattac gttgattttg gaaagtatga ggagttgatc catacggata
     3841 tcgttcacac gagtatccat gaagtaaccg ttaagggcat cagatctact ttaaaagatg
     3901 gacaggagac gaaaacaatc gaccgaagaa aagaggtcta caatttttat aaagttgaag
     3961 caagtctacg atatggcatt gggaaattga ggttagatgg gagtaatatt caacaaaaca
     4021 aaccatatgt tttaggaaaa gtggtgattt agaacttcct atgaaagatg aataaataag
     4081 gagaatcttt tgaaaatgat tcttctttac agaattgtgc ttatagttgg cacggagtta
     4141 aaaatcttaa agaaggagca gttattcaaa gtgaaagaac atcataatca gttactatgt
     4201 attaaaacgg gtacgatgaa cattcaatac cgaacagaat atttagaatt aacgaatcaa
     4261 actgattttt tgtcgattcg agagcaacat aagcaagaaa aattaatggc tacttatagc
     4321 caattgattg atgagtggga acaattcaag gaatctaaaa cataaaattc catttaaaca
     4381 ccttattttt taaagagcat aaggaaacgt gaccccactt ttaaaaaaag tggttggata
     4441 ggctgtgcct atcacgtttc tgaaaaaaat aaatgacccc aaaataccaa tatataggat
     4501 agtacaagga tagcaaagga tagtaaaagg ataggggtag gatagcgaaa aggataggct
     4561 ccttttcact atcctatatt atattggagg atatttatga atgttaaaat tagagattta
     4621 gatccaaaat tcatttctga aattgatcga agatgcgttg aactaagtaa tcgtacagga
     4681 aataaatgga gtcgtaacga ttatttaaaa ttactaatag aaaatgattt tgatcgtcct
     4741 ttgatggaat ataaaaaaga aaagtttgat cagttattag aaaagttttc agaaattcaa
     4801 agctataaca caaagatatt agaagaatat gttagccaaa acaatcgtat tttagaaatc
     4861 ttgatcgagc aaaatagagg aagtgaactt tgatatggca aaggttatag acaaacacat
     4921 ttactttcac caaacacata tcgatgtatt ggatgaaatc atttctactc atgcagaggt
     4981 taaaaactat agtgaagccg ttcggtttct atgtatgaac tatgatccag aagtggagaa
     5041 aaaagtaaat tccaatcaag caaagttgaa tggaatgagt aaagaaattt ctattttaac
     5101 cgaaatggtt tcagagtttg cagatcttca tttgaaagat acaggaatac taattggtac
     5161 aaattcaacg gtttaccaag aagcaaaaga acgtgtggaa gggaaaatta aatctaatca
     5221 aactaaaaaa tattcccctc agttgaagaa acctgtgaat gaatctgttc atgccagagt
     5281 agatccattt gatccaacga agattctaaa aagataaaag tagaaggaga acgtgatgaa
     5341 taaatcgcct gcagtcattc tcacttgtca atttaccttg cccaataaaa aatctttttc
     5401 aacatacatt gattatatga caagagaaaa agcgctggaa gaaaaggaac gacgcacgcc
     5461 cgacgaagaa caggaactgc aacgaatcaa gaatgcttta gaggattttt atattcctga
     5521 aggagaaact ttttcggaga gtaagacgga taaagaattc agcgaaacgt cgaatgaagc
     5581 tcaatctttg atgggaaatg gaatcaactt tgacaagtta gaggaacaag attttaccaa
     5641 atatctttct tatatgaccc gtcactatgc gttagaacaa aagaaagaac tgacgcaaag
     5701 tgaacaaaaa gaataccaac gcttgaacca agcaataaaa aaatatcaaa cagagccaca
     5761 caaaaaagct tcaggaaatc tccctggagt tttttctatg gcttcagacg aagtgaaagg
     5821 aagtgatctg aaggagatta agcaaatctt ccaaagaggg caacaaaaag gctcaatcat
     5881 ttatcaagat gttgtttctc atgataattc gtatcttgaa aaaataggat tgtacgatcc
     5941 caaaacagat actttagatg aagaaggatt aaaagcagct ggaagaaaaa tgatggagac
     6001 attattcgag aaagagcaac tgaacgaaac aggctactgg atggcaacga ttcaccgtaa
     6061 taccaaacat atccatattc attttgcagt tgttgaaaaa gaaaatacgc gtaaacctta
     6121 cacggaggtt gaaaatggtg tgagttatat cgtaccgaaa ggtaagagaa gacaagcaac
     6181 attagacgca atgaagacca attatgtaca tgagttagaa cgtttttctt atgaaaagga
     6241 tcgaatgatt ggtatggaaa aacgaaattt attgactcgg aaaagtgatt tgcgtaacac
     6301 cttaacaaaa gtggtaaaag agccagcgcg ttacgatcga cacgcaatga acttattaaa
     6361 tgaagtatat aaaaccctac ctgacaaacg ttcggaatgg aattacgggg atgaaaaacg
     6421 aaccaaactt tcaccataca caagagagaa actcgatgag ttaaccaaat atgttttaac
     6481 aaaagaacct gaataccaag aatatataca gatttcaaaa gcattgaaag aagaagcaaa
     6541 aagtctttat ggggaaagca agagagaatc aaagaatgca gaaaaaaacg ccctctttga
     6601 tttaaaaaag cgcacaggaa atgccatcct aacagaactg aagagacaag atagtcaaat
     6661 aaaaccatta atagaaggtg tcgatcagat gcaaactatt tacaatgaac gatcctttcg
     6721 aacacttaaa cagttgccaa caaatgaata caagtatagt tacgaagaat tcatgaagcg
     6781 aaggaaacaa ttccaacaaa gagttgtcac gcaacggagt ttgcgtcaat taaatcgaac
     6841 gattaatgcc catgaagaaa aataccacgc aagtaaagca tacgaagagc aacaacgaag
     6901 aattcaaatg gaacaagaaa gacagtaaag acagtaggag gaatagcaat gaaacaatat
     6961 ttagatcaat ggaaagttat agaaggatca ttaagagaag aacgaatcga acaacttcct
     7021 gattgtttag aaaaagagca tttgttccag ataagagaaa tgttgagaaa cgaacagttt
     7081 gatccaaatc aatttttagt cgttgagtac ccagctactg gtgtgtattg ttgtaatcat
     7141 gtgaatggtg aaaaatattt tatcattcaa gaatatgagg gcaagttagc accttattat
     7201 actacttggg aaatgaatga agagggaatc aataactttc catgtaaaag cattgaagag
     7261 tccatttcac tcacagagtg ttagttgtta agtgaataaa agaagtaaaa gagtcctgaa
     7321 cgtgattgtc caggactctt tttttcaatg aattgttttc cgccaactca ttgagtggtg
     7381 cgatttgcga ctacattata tcaaaagaaa gagaagggag gaagcagaaa tggttcattc
     7441 aacgattgga gttgttgttg aaaaaagcag agacaatctc gtttttgtga cagaaattca
     7501 aacaggcaga tcttttgtcg tcacagacaa agcggcaaaa gcttatcaaa gtagcgatat
     7561 tttaacgtta aatctgacta cgaagacgtt cgtcgatgcg
//
LOCUS       DAFEOM010000102         7457 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.107, whole genome shotgun sequence.
ACCESSION   DAFEOM010000102
VERSION     DAFEOM010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.107"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            283..1386
                     /locus_tag="LP524_002587"
     CDS             283..1386
                     /locus_tag="LP524_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBL2235584.1"
                     /translation="MTEKGRSYLKEQRKFPEWLIDYGEQQGLILEMKPRNERKNYIVQ
                     DDRLDYAIVFPWKDRHTDQILGATYQGTIVDYERFGKRGVDKRIDINPTPNHGFNVLL
                     GSPKNLKFFESPIDLLSYAALNRETIKDTWLVSMDGLKDAVVGHYVREAVTELAPKEE
                     FPESIEICVDNDRAGHVFWDKVHRLGVSNPFTGKAVYFEPNLPNDWQVPREYQTIYEE
                     VGKEMRVAPEAIMAIHKIENNLTDTNQIVSFGEIQGVFAKKLAPKEKAQSIVVKEKCI
                     EAAQQLKSCEKSDGTYDFDRFYRGKGKINEQIQFSLKAKHYFEGYKNHEHEFVSEVKK
                     DWNDQLKHEIQQQAIRKQKRAMLFQQGRQQERE"
     gene            1397..3601
                     /locus_tag="LP524_002588"
     CDS             1397..3601
                     /locus_tag="LP524_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBL2235585.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDEKWKQWSLAHLPIFPNQYHYHVPKDKKKQFNVVKRQLQSADT
                     VVIATDSDREGELIAWSIIQQAGADQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAHVITQNGAFDAKLDPNETQATQEAFEAYLKDKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEEKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPVDLGWKKLFGK
                     ESDGKEKEEEPLLPKVAKGEMVTVDLQVLEKETKPPKPYTEGTLITAMKTAGKTVDSE
                     KAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            3617..3766
                     /locus_tag="LP524_002589"
     CDS             3617..3766
                     /locus_tag="LP524_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235586.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            3840..4433
                     /locus_tag="LP524_002590"
     CDS             3840..4433
                     /locus_tag="LP524_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL2235587.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKEAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDEYAIQPHYTNKENFDLGITETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            4449..5348
                     /locus_tag="LP524_002591"
     CDS             4449..5348
                     /locus_tag="LP524_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBL2235588.1"
                     /translation="MTPTQFKAKTRNFATEKHLNPQIVQRYYMMEKLLERIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTADPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKKLYNQEMPQLMKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKVERKQNFKRSQQMNQEMER"
     gene            5351..5836
                     /gene="ssb"
                     /locus_tag="LP524_002592"
     CDS             5351..5836
                     /gene="ssb"
                     /locus_tag="LP524_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL2235589.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTATFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            5858..6115
                     /locus_tag="LP524_002593"
     CDS             5858..6115
                     /locus_tag="LP524_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235590.1"
                     /translation="MAKKNSLTMDLLIPVIAFIDGISLGITENLLVNRIAKGSLFLVF
                     VYLVCSYLLKRVGKDNSFWRLTKVTIGATAVILTAILPLYM"
     gene            complement(6275..6397)
                     /locus_tag="LP524_002594"
     CDS             complement(6275..6397)
                     /locus_tag="LP524_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HBL2235591.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            6551..6811
                     /locus_tag="LP524_002595"
     CDS             6551..6811
                     /locus_tag="LP524_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235592.1"
                     /translation="MEHYTGLLDKIRVIKTQPLLVRFTLHTSHEPINCVVANIEIADK
                     LLILEEGKYTISVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            7254..7454
                     /locus_tag="LP524_002596"
     CDS             7254..7454
                     /locus_tag="LP524_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBL2235593.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PICGKKYTLSYGDYILIEEKGR"
BASE COUNT         2680 a         1304 c         1557 g         1916 t
ORIGIN      
        1 ttagcggtag tcaataaagg acgagactat cgtttagaag accatgattc ttttgttttc
       61 gatcgaagaa aacagcgatt ttattggaac agtcaaggta ttggtgggga tattattgaa
      121 ttgactcaat tgttttttat tgacgattct attcaaaacc caaagcaacg ttttaaagca
      181 gcgctagatt atattataaa ggacgaaaat aaggtggaac gggtggaaaa tcttcacttt
      241 gaaacacaag aatataagca ccacccaatt gattatcgac cattgacaga aaaaggacga
      301 agttacttaa aagaacaacg aaaattccct gaatggttga ttgattacgg agaacaacaa
      361 gggttgatcc tggaaatgaa gccacgcaat gaacgaaaaa attatattgt gcaagatgat
      421 cgcttagact atgcgatcgt ttttccctgg aaagaccgtc atacagacca aatattaggg
      481 gcaacttatc aagggactat cgtagactat gaacgttttg gaaaacgggg tgttgacaaa
      541 cgaatagata taaatcctac gcctaatcac gggtttaatg ttcttcttgg atctcctaag
      601 aatctgaaat tttttgagag tcccattgat ttacttagtt acgctgcttt aaatcgcgag
      661 acgatcaaag acacgtggct tgtttctatg gatggactga aggatgcagt agtggggcac
      721 tatgttcgag aagcggtgac agaattggcg ccgaaagaag aatttccaga atcgattgag
      781 atttgtgtgg ataatgatcg agctggacac gtattctggg ataaggtaca ccgacttggt
      841 gtgtcgaatc catttacagg aaaagcggtg tattttgaac cgaatcttcc caacgattgg
      901 caagtaccga gagaatatca aacaatctat gaagaagtag ggaaagaaat gagagttgcg
      961 cctgaagcaa tcatggctat tcataaaatc gaaaacaacc taacggatac taaccaaatc
     1021 gtatcgttcg gtgaaatcca gggagtattt gccaaaaaac ttgcgccaaa agagaaagcc
     1081 caatctattg ttgtaaagga aaaatgtata gaagcagctc agcagctaaa atcgtgtgaa
     1141 aaatctgatg gaacatacga ttttgatcgt ttttatcgtg gaaaagggaa aattaatgaa
     1201 caaattcaat tttcattgaa ggccaaacac tattttgaag gttataaaaa tcatgaacac
     1261 gaatttgttt ctgaagtgaa aaaagattgg aatgatcaat tgaagcatga gatccagcaa
     1321 caagcaatca gaaagcaaaa acgtgcgatg ttgtttcaac aaggcagaca acaagaaagg
     1381 gagtgaaatc gtcatgatga aaaccgttat tttagcagaa aaaccgtcac aagcgaaggc
     1441 ttatgccgat agcttttcta aagctacccg aaaagacggg tattttgaaa tccaagatcg
     1501 gttgtttcca ggagaaactg tgatcacgta tggttttggt catttagtcg agttagattc
     1561 gcccgatatg tacgatgaaa aatggaaaca atggtcatta gcgcatttgc ctatttttcc
     1621 aaatcaatac cactatcacg ttcctaaaga taagaaaaag cagtttaatg tcgtcaaacg
     1681 ccagctgcag tcagctgata cagttgtgat cgcgactgat agcgatcgtg aaggggagct
     1741 gattgcctgg tcaatcattc aacaagcagg cgccgatcaa ggaaaaacct ttaaaagact
     1801 ttggatcaac tccttagaaa aagaagccat ttatcaggga ttccaacagt tacgagacgc
     1861 agaagagacc tatccaaaat ttgaagaagc acaagcacgt cagatcgccg attggttaat
     1921 tgggatgaat ggtagcccac tgtatagttt actcttgcag caaaaaggca taccaggaag
     1981 tttttcctta ggcagagtcc aaaccccaac gctctacatg atttatcaat tgcaggaaaa
     2041 gattagaaac ttccaaaaag aaccatattt tgaaggaaaa gctcatgtaa tcactcaaaa
     2101 tggcgcattt gatgcgaagc ttgatccgaa tgaaacccaa gcgacgcaag aagcatttga
     2161 agcttattta aaagacaaag gtgttcaatt aggaaaacag ccaggtacga tccttcaagt
     2221 cgaaacagag aaaaaaagtg cggctagccc acgtcttttt tctttatcga gccttcagtc
     2281 taaaatgaat caactcatga aagctagtgc caaagatact ttggaagcca tgcaagggtt
     2341 gtatgaagag aaatatctaa gctatccgcg aacggatacc ccttatatta cggaagggga
     2401 atatgcgtat ctgttggatc acttagacga atacaagcat tttttgaggg ctgaagaaat
     2461 tccaacccct attcatacgc cgaatagtcg gtatgtgaac aataagaaag tccaggaaca
     2521 ttacgcgatc attcctacaa aaacggttat gacagcagcg gcttttgaac aattaagtcc
     2581 actgcagcag gctatttatg aacaagtgtt aaaaacaacg gtggccatgt ttgcggaaaa
     2641 gtatacctat gaagaaacaa cgatcttgac gcaagttcag cagcttcagt taaaagcaat
     2701 cggaaaagtg ccggtagatc taggttggaa gaagttattt ggaaaagaga gcgacggaaa
     2761 agagaaggaa gaagagccgc tacttcctaa agtggcaaaa ggagaaatgg tgacggtaga
     2821 tctacaagtt cttgaaaaag aaacaaagcc accaaaacca tataccgaag gtacgttgat
     2881 cacggcgatg aaaacagctg ggaaaaccgt ggatagtgaa aaagcgcaat ccatattgaa
     2941 agaagtggaa ggaatcggta cagaagcgac acgggcgaat attattgaaa ccttgaaaca
     3001 aaaggaatat atcaaggtag agaaaaataa gctcgtggtg acgaataaag ggattttgct
     3061 ttgccaagcc gtggaaaaag agccgctgct tacgagcgcc gaaatgaccg ccaaatggga
     3121 aagctacttg ttaaagattg gagaacgaaa aggaacacaa accacttttc tagccaatat
     3181 ccaaaaattt gtctctcact tattggaagt cgtcccagga caaatccaat ccactgattt
     3241 tggatcgacg ttacaagaag tgaaagcagc cagtgaaaag caagaagccg cgcgtcattt
     3301 aggaatttgt ccaaaatgcc aggaacaaga agtcttactc tatcaaaagg tggcagcttg
     3361 tacgtctgaa gcctgtgatt ttaaattatg gacaacgatt gccaaaaaga aactcacagc
     3421 gacacagctg aaggagatta ttcaaaatag ccgaacaagc caaccagtga aagggttaaa
     3481 aggtcagaag ggttcgtttg aagcaacgat tgttttaaaa gaagacttca ctacaggctt
     3541 tgaattttct gaaaagaaaa aaacgaactt caagaaaaga acacgacgca ctacaaaata
     3601 atggagggtt aatcttatgc gtaatacaac acacaaacaa caagaaatcg aaaatcaaaa
     3661 agccaaaatt cgttcagaac aggaaaaaat gcgtgccagc aaacgaagac tgcagcaatt
     3721 aaagaaccag caacggattc aaaaagaaat tgaattagat cgataaatat ctttcttgtt
     3781 aatataaaac taacgtgata tagttatgtt agttttatat taactttagg ggaaaagcaa
     3841 tgaaagacaa aatccagtgc atttttgata aacataatgg gaatctatcc ttgaaggatg
     3901 ctagaagtga aggcttatcg cctatgacgt tagatcgatt agccgaaaag gacaaaatag
     3961 aaaaggtagc accaggtttc tataccttaa aagaagcgtt cgtggatgaa ttgtatctcg
     4021 ctcaaagtat ctattccaga ggtatttttt ctcatgagac tgcgctcgat ctttttcaag
     4081 tcggaacgta tatccctaaa aagatcaata tgatgtttcc taaaggatac aatgtttcaa
     4141 aggagaaatt agacgagtat gctattcaac cgcattacac gaataaagaa aattttgacc
     4201 tgggaatcac agaaacagaa agcttttacg gcaataaaat tttagtctat gatttagagc
     4261 gaacgttatg tgatatgtgg aatccaagat acaaagcaag cgtagaggta aaacaagaag
     4321 cattaaaaga atatatgaca agtaccagta gaaatacaaa taagctaaga agatacatgg
     4381 atattttaaa atcaccaaaa gaaatgactt tgtatatgtt gccactttat tgagaggagt
     4441 aaaggtgaat gacaccgaca caatttaaag caaaaacgag aaatttcgca acagagaaac
     4501 atttaaatcc acaaattgtc caaagatatt atatgatgga aaaattatta gaacgtattg
     4561 ttgaatcgcc ttatcatgat gattttgtat taaaaggggg atttctgatc ggatcaaaat
     4621 acgggttaga aaaccgttca actgttgata ttgatacgac ttatcgaaat tcgaaactaa
     4681 caaaagaaaa gttagaaact gttttttctg agttaacagc tgatcccaca aaagatggaa
     4741 ttcgatttga attaaaggga ttaacagaga cgagggaagc cgattattac ccaggttttc
     4801 aagcaaagtt tattgcttat cttgagaatg cacgtgtacc atttaaatta gatattactt
     4861 caggtgattc tattattcca gcggcattga tgtatgaaca caaattaatg tttgaagata
     4921 aaacgattca tattccagct tatcctacgg aacaaattct agctgaaaaa ttacatgcta
     4981 cgtttagttt cggaaaagat aattcgcgca tgaaagactt ttatgacctg tatatgattc
     5041 caaaactaga acgtatcgat gagaaaaact tgtataagag tgttcaagga acgttcaagg
     5101 aaagaaataa tcaatctgtg tttaaaaaat tgtataatca ggaaatgcct caattgatga
     5161 aggatgaggg gttgagggaa aagtggaaaa gataccaaca agaaaattat tttgccaaag
     5221 acatttcttt cgaatctaca ctaacttccg tcgattcttt gatgcaatca attgtagagc
     5281 aagagaaagt agaacgaaaa caaaatttta agagatcaca acaaatgaat caagaaatgg
     5341 agagataaaa atgattaata atgtaacttt agtaggacga ttaaccaaag atccagattt
     5401 acgttatact gcaagtggta ctgcagtagc gacttttacc ctagccgtta accgaaattt
     5461 cacgaatcaa aacggcaatc gagaagctga ttttatcaat tgcgtgattt ggcgcaaacc
     5521 agcagaaacg atggcgacat tagctaaaaa aggcagcttg atcggtgttg tcggacggat
     5581 tcaaacacgg acttatgaca accagcaagg acaacgtgtg tatgtgactg aagttgtagc
     5641 agataacttt caactattag aaagtaaagc agcgacagag agccgtgcgc atgcagatca
     5701 atcgtccacc agtccaagca ccgccacctt tgaacaacgg gatacagcga cgcctaacaa
     5761 caatggattg aacgcttcac agaatccatt tggtggacaa tcaatcgata ttagcgatga
     5821 tgatctaccg ttttagttcg agaaagaagg agtaagcatg gcaaaaaaaa atagtctgac
     5881 gatggatcta ttgatacccg taattgcatt tatagacggg atttctttgg gtatcactga
     5941 aaatcttttg gtaaatagaa ttgcgaaagg atcactattt cttgtattcg tgtatcttgt
     6001 ctgttcttat cttctaaaaa gagtaggaaa ggataattcc ttttggcgat tgactaaagt
     6061 cacaataggc gcgacagctg ttatacttac ggctattcta ccgctttata tgtagggtta
     6121 acgggaaatc attgaacttc ttgcgaaata ttgttataat gaacatgaaa aagaggagtt
     6181 gcgcaaacaa cccctcatta acagcaccgt ttaaagaacg gcggctcaga ttaattattt
     6241 attgagaaat aaccgatcct cgctaaagtg acggttattt ctttttgtca tttttaagca
     6301 tatttacaat caaactaatc aaagcaatgg taaacatacc aaaaccaaga atggtttgaa
     6361 ttgtctcata tgcggacaaa aggcttactc ctttcataga ttttagcact tacattcata
     6421 agcaccacca ccttccagaa gtcgccaccg tcattaaact ttactgtctt gatcattata
     6481 gcatatctaa aatcgaataa aaattctcaa ttttagctgt ttttgctata attttagcga
     6541 ggtgatcgcg atggaacatt atactggttt actcgataaa attcgggtga tcaagacaca
     6601 gccattgctt gtgcgattta cattacacac gagtcatgaa ccaatcaatt gtgtagtggc
     6661 caatatagaa atagcggata aattgctgat tttggaagaa ggcaagtata ctatttcagt
     6721 gacaggtcac ttcaataaac ggaatcaatt agtcattgaa tcgatgcaaa ttagaaaccc
     6781 cgatcatttt acgcgatcga tgggcatata aaagaagagc tgcaatccta atgtaggact
     6841 gcagctcttt ttgttttaca tagaaacggg aacaggaaaa ggccttttta ggaaggcaca
     6901 agtgccttct caattgaaaa acctttctct cactccaacc gccaccgccg ttcgagaaga
     6961 aaggaagtga acgctgcacg ttcactaaga taaggggtct tgcaaagcaa gacaaaatag
     7021 aaaaaacttc ctctcaccaa aaggttcgtt cctgttcgtt atctctttat tgatttgcca
     7081 gaccggcaac aagttgcctt ttatcaccaa aaagcgacaa ggacaagggt aaatggcacg
     7141 ctgcgcgccc ttgtccttgt cgcttttcgt tgattcattc tggccaacaa atcaataaag
     7201 aaagaactaa cgaaaggaag taaaaccttt cctaagagga ggcagaatca aacatgcgaa
     7261 agaaatttga ttattggggt gttcccttca gcgaattatt cccaaactat catgcaccac
     7321 atactgtcga atgcgattgt ggagaaagag cgaaatgtat caaaagctat cgtttgtatc
     7381 aatgtccaat atgcgggaaa aaatatacct tgtcttacgg cgattacatt ttgattgaag
     7441 agaaaggaag ataaaat
//
LOCUS       DAFEOM010000103         7149 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.108, whole genome shotgun sequence.
ACCESSION   DAFEOM010000103
VERSION     DAFEOM010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.108"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            83..346
                     /locus_tag="LP524_002597"
     CDS             83..346
                     /locus_tag="LP524_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system, antitoxin component,
                     AbrB family protein"
                     /protein_id="HBL2235594.1"
                     /translation="MLVKEIEMKKWGNSQGIRFGKEELSVLGETNTENLKFEMVVTEG
                     KIVLTPKNKKPETLDELFANYEGEPLGKEDKYEWGNMQGREII"
     gene            346..681
                     /locus_tag="LP524_002598"
     CDS             346..681
                     /locus_tag="LP524_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBL2235595.1"
                     /translation="MKKNYHDLSQGDLVYIDLNPTKGHEQRGKRPCIILSKPNKYLNY
                     MIGVAPITTKAKPFPVHVDLNGTQKIKGQILLEHHRMIDIESRGFQFVEAVPKEILEE
                     CIEKIKLFY"
     gene            755..1957
                     /locus_tag="LP524_002599"
     CDS             755..1957
                     /locus_tag="LP524_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBL2235596.1"
                     /translation="MENDTNIVMEHLKRRAKRRLFLWLFGTSAGLITILITVFVTLFL
                     ILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKL
                     LPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFP
                     NSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMSI
                     ITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGGE
                     FRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQ
                     EFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPL
                     PFLNSSKK"
     gene            1973..2617
                     /locus_tag="LP524_002600"
     CDS             1973..2617
                     /locus_tag="LP524_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235597.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQANIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDNHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVVDFSYFTR"
     gene            2628..3434
                     /locus_tag="LP524_002601"
     CDS             2628..3434
                     /locus_tag="LP524_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235598.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            3456..3686
                     /locus_tag="LP524_002602"
     CDS             3456..3686
                     /locus_tag="LP524_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235599.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTAERK"
     gene            4155..4451
                     /locus_tag="LP524_002603"
     CDS             4155..4451
                     /locus_tag="LP524_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235600.1"
                     /translation="MNVKIRDLDPKFISEIDRRCVELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQEKFDQLLEKFSEIQSYNTKILEEYVSQNNRIIEILIEQNRGSEL"
     gene            4453..4875
                     /locus_tag="LP524_002604"
     CDS             4453..4875
                     /locus_tag="LP524_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235601.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDEIISTHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGKNSTVYQEAKERVE
                     GKIKSNQTKKYSPQLKKPVNESVHARVDPFDPTKILKR"
     gene            4894..6486
                     /locus_tag="LP524_002605"
     CDS             4894..6486
                     /locus_tag="LP524_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235602.1"
                     /translation="MTQSPSVILTCQFTLPNKKSFSTYIDYITREQALEEKERRTPEE
                     EQELQRIKNALEEFYIPEGETFSESKTGKEFSETSNEAQSLMEHGINFDKLEEQDFTN
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSIASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            6507..6842
                     /locus_tag="LP524_002606"
     CDS             6507..6842
                     /locus_tag="LP524_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235603.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGNLVPYYTTWEMNEEGINNFPCKSI
                     KESISLTEC"
     gene            6979..>7149
                     /locus_tag="LP524_002607"
     CDS             6979..>7149
                     /locus_tag="LP524_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235604.1"
                     /translation="MVHSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGNI
                     LTLNLTTKTFVDA"
BASE COUNT         2726 a         1052 c         1473 g         1898 t
ORIGIN      
        1 aaattgtttg atggtggaaa gtaacttctt gtattttggt gtatacaagt atatactaat
       61 gtgtgagaga aggagggatt ccatgttagt aaaagaaatc gaaatgaaaa aatggggaaa
      121 ttcacaaggt attcgttttg gaaaagaaga attgtcagtt ctaggagaaa ctaatactga
      181 aaatttaaaa ttcgaaatgg tggtaacaga aggaaaaatt gttttaactc ctaaaaataa
      241 aaagccagaa actttagatg aattgttcgc caattatgaa ggagaaccct taggaaaaga
      301 agacaaatat gaatggggaa atatgcaagg tagggagatc atttaatgaa aaagaactat
      361 catgatttat cgcaaggaga tcttgtttat attgatttaa atcctaccaa aggacatgaa
      421 caacggggaa aacgaccatg tattatttta tctaaaccta ataaatattt gaactatatg
      481 attggtgtcg caccgataac aacaaaagcg aagccttttc ctgtacatgt tgatttgaat
      541 ggtacgcaaa aaataaaagg acaaattctt ttggaacacc atcgaatgat tgacattgag
      601 agtagaggtt ttcagtttgt ggaagctgtg ccaaaggaaa ttttagaaga atgtattgaa
      661 aaaattaaat tattttatta aaagcagcga agaataatta ttatttcttc gcttttttgt
      721 tgtttaaaat taaaaagtta ggagtactga ctatatggaa aatgatacaa atatagttat
      781 ggaacatttg aagcgtcgtg caaagcgacg cttatttttg tggttgtttg gtacttctgc
      841 aggtttgatt actattttga tcactgtgtt cgtaaccctt tttctaatac ttgcggcggg
      901 ttcgattgat aattcggatt ccgattcaag tagtggtggc gaagctttta caggagagta
      961 ttcggaaggc ttaccgattt ataaagaaat caaaggaaga ggcccttttt ctgatgagat
     1021 tgcgcaatat gccgtaggtg cagcagtgaa atataaatta ttaccaagtg tgattctttc
     1081 gcaatacggt tatgaatcag cgtttggtac gtcggcgtcc gcaagaaatg atctcaacta
     1141 ttttgggatt acttggtttg atggttgtct gtttcctaaa ggaactgcac gaggaattgg
     1201 tggcattgaa gggggctggt acatgaagtt tcccaactca aaagctgcat tctcctatta
     1261 cggctttatg gtagccacac aatcaaattt taatgcatgt gtaggaaata aaagccctgg
     1321 cgcaagctta ttgatcttag gacgaggtgg ttatgccgct gcaggaatta ctgaagatag
     1381 tccgtattat acaggttgta tgtcaattat cacttctaat aaattaaccg aatatgatga
     1441 attcgccatc aaacattggg gtgaaggtgg caataacaat ggcacaatta ctggtgaatg
     1501 gacgaatcca ttccctggca gcagtttaga taaaagttcc ttcagcggtg gtcaattatt
     1561 tggaacgaat ccaggcggtg aattccgtcc gaacggcttc catgatggat tagattttgg
     1621 ttcagttgat catccaggaa gtgagattca tgcggttcat ggcggaaaag tcgtgtacgt
     1681 tggcaatcct ggtatttcag gtttgggcgc gtgtgtgatc gtgattaatt acgatggctt
     1741 gaatatggtg tatcaagaat ttgcaaatag tacaggaaat tctcgagtga aagtaggcga
     1801 tcaagtgaaa gttggtcaag taattgggat acgagataca gcacacttgc atttaggatt
     1861 tactcgaatg gattggcgtc aagcacaagg acatgcattc attgatgatg gtacatggat
     1921 cgatccgtta ccatttttga atagcagtaa aaaatagatc aggagaaaaa atatggaaaa
     1981 taaaacgtta aaaatcgtct taacagctgc aattagcgtt agtttaatcg gcaatattgc
     2041 gttgttttgg cagctgaaag aggaacaagc agcaaatacc tataaaattg aagaagtggc
     2101 taaaaacgcg tctgagaagc tcaaaaaaaa ttcctctgag gcacaggcga acatcgacaa
     2161 gttaaatcaa accatcaatg agaaaaacca agaaatcgat cagctgaagc aacaagatgg
     2221 cacaaaagca aaagaggaag taactgaagc acaaagaaag acggcagaag agttcggtca
     2281 attggccatt gataaatcat taagcagaaa tgaattaacg gaaaaactaa aagatgtcgc
     2341 aacccaagaa gtgatcgaca aattaagtcc tgcagacgat aatcaccaac atgatgatta
     2401 cggaagttac tcctttacat taggtgacgt gcaaacttac gctcaaacaa gcagtgtaaa
     2461 agaggaacaa aattttgtcg tatttgttga ttatacaatc ggcaatccgc aattcaaaga
     2521 tgtcaaacca caaaaaataa aaggtggatt aactgtcact gaaaaacaag tagatggtca
     2581 atggaaagtc gtagacttta gttatttcac tcgttaggag gaagaaaatg agtaagaaaa
     2641 aaggagaaag acaacgtttc aaaaaacgaa aaagttatct tcaatggctg ggaatcttta
     2701 tgattctcgg ctttttgatt cttgctagtc cgatttatac gaatcagaag gtagatatac
     2761 cagatgaggt aagcacgaac gcctttttaa acacgaaaga gctgacaatg gtttcaaaag
     2821 aaaaaggagc gaaccagcaa ttagtttttg aatttgcgat cgacgatgaa gatcaaagct
     2881 tgttaaaaga actggctaat cttaattatc gcgtagaagt aaagacagct aaaggggatt
     2941 atcaagcaat acgagccaaa gtgatcaagg tgtctgatga ttattttgca gtgaagctaa
     3001 cgcatgtacc agaagaatat atcgccatgc gtttaaccat tattcctgaa aaaattgatc
     3061 caaaagtgga tatgcaagaa ccacaagatc tgatttatta cgtgcacgaa gataaggtta
     3121 aagatcaagt gaaagatgaa gattatgaac aacacgcgat caattacaaa gtgaaaggat
     3181 acaagaaaga acaaaaagca gctcaaaaac aaatggaatc tttccaagca accattgatt
     3241 tgaatgaaga attagtgaag aagctaaaag atcaattgcc ttatcaagtc gcagatgatc
     3301 aggaaaatac tgaaaacaag attaatagct accagcaaga aattgaaaca tcgaaaacgc
     3361 aactgaaaga tcaagaagag atcattcaaa aacttcaaga aaaaattgat cgtatgacaa
     3421 aagaaaagat ttagaaggac aggtggagtg aaaaaatgaa gcgaacaaac ttggctgaca
     3481 aagatacttt cgctgagcaa aaagtattga ttaaagccta tctcaaagtt gtaaaagcta
     3541 aagaaatatg tcgagaagtg aatttatcta acacaacaat atctgagttg aagaatggtc
     3601 gaaagaattt agatacttta aaattcagat cgtttgaagc tctatatgta gctgctaaaa
     3661 aatattttac ggctgaaagg aaataaaaac aggaggattt tatgtgaaaa aagtatatca
     3721 aaaatgttta tggatcagct atatcaccgt aacacaaaca tcaagcacat aaaacattta
     3781 acactcctcc tttctggctg aagatttcaa ccaaacagga agacagcgta tcaattatag
     3841 catacacttt ttcttaaatt catttttcat atgacaaaat atttccttct aatcgacaaa
     3901 gttagaagga ttttttattg tctaaataca caaataacct taatttttaa agagcataag
     3961 gaaacgtgac cccactttta aaaaaaagtg gttggatagg ctgtgcctat cacgtttctg
     4021 aaaaaaataa atgaccccaa aataccaata tataggatag tacaaggata gcaaaggata
     4081 gtaaaaggat agaggtagga tagcgaaaag gataggatcc ttttcactat cctatattat
     4141 attggaggat atttatgaat gttaaaatta gagatttaga tccaaaattc atttctgaaa
     4201 ttgatcgaag atgcgttgaa ctaagtaatc gtacaggaaa taaatggagt cgtaatgatt
     4261 atttaaagct actcgttgaa aatgattttg atcgtccttt gatggaatac aaacaagaaa
     4321 agtttgacca gttattagaa aagttttcag aaattcaaag ttataacaca aagatattag
     4381 aagaatatgt tagccaaaat aatcgtatta tagaaatctt gattgagcaa aatagaggaa
     4441 gtgaactttg atatggcaaa ggttatagac aaacacattt actttcacca aacacatatc
     4501 gatgtattgg atgaaattat ttctactcat gcagaggtta aaaactatag tgaagctgtt
     4561 cgctttttat gtatgaacta tgatccagaa gtggagaaaa aagtaaattc caatcaagca
     4621 aagttgaatg gaatgagtaa agaaatttct attttaaccg aaatggtttc agaatttgcg
     4681 gatcttcatt tgaaagatac aggaatatta attggtaaaa attcaactgt ttatcaagaa
     4741 gcaaaagaac gagtggaagg aaaaattaaa tccaatcaaa caaaaaaata ttcccctcag
     4801 ttgaagaaac ctgtgaatga atctgttcat gccagagtag atccatttga tccaacgaag
     4861 attctaaaaa gataaaaatg aaggaggata ataatgactc aatcgccttc agttattctc
     4921 acttgtcaat ttactttgcc gaataaaaag tcattttcta cttatattga ctatattaca
     4981 agggaacagg ctcttgaaga aaaggaacga cgtacgcccg aagaagaaca ggaacttcaa
     5041 cgaatcaaga atgctttaga ggagttttat attcctgaag gagaaacttt ttcggagagt
     5101 aagacgggta aagaattcag tgaaacgtcg aatgaagctc aatcgttgat ggaacatgga
     5161 atcaattttg acaagttaga ggaacaagat tttaccaatt atctttctta tatgacacgt
     5221 cactatgcgt tagaacaaaa gaaagaactg acgcaaagtg aacaaaaaga ataccaacgc
     5281 ttgaaccaag caatgaaaaa atatcaaaca gaaccccata aaaaagcttc aggaaatctt
     5341 cctggagttt tttctatagc ttcagacgaa gtgaaaggaa gtgatctgaa ggagattaag
     5401 caaatcttcc aaagaggaca acaaaaaggc tcaatcattt atcaagatgt tgtttctcat
     5461 gataattcgt atcttgaaaa aataggattg tacgatccca aaacagatac tttagatgaa
     5521 gaagggttaa aagcagctgg aagaaaaatg atggagacat tattcgagaa agagcaactg
     5581 aacgaaacag gctactggat ggcaacgatt caccgtaaca ctaaacatat ccatattcat
     5641 tttgcagttg ttgaaaaaga aaatacgcgt aaaccttaca cggaggttga aaatggtgtg
     5701 agttatatcg taccgaaagg taagagaaga caagcaacat tagacgcaat gaagaccaat
     5761 tatgtacatg agttagaacg tttttcttat gaaaaggatc gaatgattgg tatggagaaa
     5821 cgaaatttat tgacgcggaa aagtgatttg cgtaacacct taacaaaagt ggtaaaagag
     5881 ccagcgcgtt acgatcgaca cgcgatgaac ttattaaatg aagtatataa aaccctacct
     5941 gacaaacgtt cggaatggaa ttacggggat gaaaaacgaa ccaaactttc accatacaca
     6001 agagagaaac tcgatgagtt aaccaaatat gttttaacaa aagaacctga atatcaagaa
     6061 tatatacaga tttcaaaagc attgaaagaa gaagcaaaaa gtctttatgg ggaaagcaag
     6121 agagaatcaa aggatgcaga gaaaaatgct ctctttgatt taaaaaagcg cacaggaaat
     6181 gccatcctaa cagagctgaa gagacaggat agtcaaataa aaccattaat agaaagtgtt
     6241 gatcagatgc aatctattta caatgaacga tcctttcgaa cgcttaaaca attgccaacg
     6301 aatgaataca aatatagtta cgaagaattc atgaagcgca ggaaacaatt ccaacaaaga
     6361 gttgtcacgc agcggagttt gcgtcaatta aatcgaacga ttaatgccca tgaagaaaaa
     6421 taccacgcaa gtaaagcata cgaagagcaa caacgaagaa ttcaaatgga acaagaaaga
     6481 cagtaaagac agtaggagga atagcaatga aacaatattt agatcaatgg aaagttatag
     6541 aaggatcatt aagagaagaa cgaatcgaac aacttcctga ttgtttagaa aaagagcatt
     6601 tgttccagat aagagaaatg ttgagaaacg aacagtttga tccaaatcaa tttttagtcg
     6661 ttgagtaccc agctactggt gtgtattgtt gtaatcatgt gaagggtgaa aaatatttta
     6721 tcattcaaga atatgaaggg aatttagttc cttattatac tacttgggaa atgaatgaag
     6781 agggaatcaa taactttcca tgtaaaagca taaaagagtc tatttcactc acagagtgtt
     6841 agttattaag tgaaaaaaag agtcctgaac gtgattgtcc aggattcttt ttttcaatga
     6901 attgttttcc gccaattcat tgagtggtgc gatttgcgac tacattatat caaaagaaag
     6961 agaagggagg aagaagaaat ggttcattca acgattggag ttgttgttga aaaaagcaga
     7021 gataatctcg tttttgtgac agaaattcaa acgggcagag cttttgtcgt cacagacaaa
     7081 gcggcaaaag cttatcaaag tggcaatatt ttaacgttaa atctgactac gaagacgttc
     7141 gttgatgca
//
LOCUS       DAFEOM010000104         7045 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.109, whole genome shotgun sequence.
ACCESSION   DAFEOM010000104
VERSION     DAFEOM010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.109"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(389..802)
                     /locus_tag="LP524_002608"
     CDS             complement(389..802)
                     /locus_tag="LP524_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HBL2235605.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1258..1743)
                     /locus_tag="LP524_002609"
     CDS             complement(1258..1743)
                     /locus_tag="LP524_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235606.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(1882..2127)
                     /locus_tag="LP524_002610"
     CDS             complement(1882..2127)
                     /locus_tag="LP524_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBL2235607.1"
                     /translation="MFYHLRKENSNENLDAIALTLLIVGGLNWLLVGLFELDLVAMIA
                     GGSTTIFAKIIYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2320..3120
                     /locus_tag="LP524_002611"
     CDS             2320..3120
                     /locus_tag="LP524_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL2235608.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3139..5007
                     /locus_tag="LP524_002612"
     CDS             3139..5007
                     /locus_tag="LP524_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBL2235609.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5000..5491
                     /locus_tag="LP524_002613"
     CDS             5000..5491
                     /locus_tag="LP524_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HBL2235610.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            5479..6033
                     /gene="srlA"
                     /locus_tag="LP524_002614"
     CDS             5479..6033
                     /gene="srlA"
                     /locus_tag="LP524_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBL2235611.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6051..>7045
                     /locus_tag="LP524_002615"
     CDS             6051..>7045
                     /locus_tag="LP524_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBL2235612.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2475 a         1126 c         1364 g         2080 t
ORIGIN      
        1 tctgaaatat tctaacacaa atatgaaaaa gatggccgaa gctaataagc cgatttcgta
       61 gcaaaacaat gagataccag agtcgtaata ataaaatcct ctaatcttat ggataaaata
      121 gatcatcgga caagcagata agagtaagta tttgttaggc cttttgaata agtaaaagaa
      181 agcactggtc attatgataa atggtatcca aaacaaaagt ttacttaaga caatcaaatg
      241 atcaactccc atatttttta ccaagagaga tcataacatc tttatcgtaa aaacaaaata
      301 tttttttgct cattttgaaa atttaaacat actcgttctt atccaaatat ataaaaatcg
      361 cccctcattc atcgagaggc gataagttct aatttaactc aatttttttt gtttcttttg
      421 gctgataagc tgttttaggt aataccaatt tcagcacacc gtttttaaag gttccagtga
      481 ttttttcttc atcaacatta ggaagataga atgaacgttt gtaagagacc gaatgtcttt
      541 ctttccgtac atagttgcca tcttctttat cttctgtatg ggactcatga ttggcggaga
      601 tcgtcaaagt attatctgaa tattcaactg tcgtgtcttc tttgtcacag ccaggcatat
      661 cagcagttag tttgtattct ttttcatttt cgactaaatc tacttttgga taacttgaaa
      721 cacctaaaaa atcattgaat gctggactaa acacgtcatt gaaatcaggg aacatgtcgc
      781 gaatgctagg tacatttgcc atttttgatc atcctttcaa taagtatttt ttatttatcc
      841 ctatttacgt aataactcta ttttgaaaaa aatgcaagta taatggttag ctttgtgaat
      901 atttcatcta atacgataca aaggctatct ttctaatttg aaaaagggat cattaaaata
      961 ataagaagaa aatatgtcaa aaggtaaaaa agtaaattct tctatccaaa tatagaaaaa
     1021 ttgaatctag aaaaccgccc gacaataaat ttcattaact cagttgaccg aaaatgaagt
     1081 agataccaaa aatttatcaa gagagcaagt agagaagtga gtatattcat aagagaaata
     1141 gctgtaacga attattaggt taatgcaaat gcttatttat actccgtata actttctcag
     1201 aaaaaatgat accaataatg acttgctagt actgtaaatc cgtataatat catctaatca
     1261 aaatggtgtt ccatttgcat ctgtcatcgt aatcgatcct tcgggtgaca ctcgataaaa
     1321 accaacgctt ccagatcctc cttgggaacg gatttgtttg ctggaagctg tgaacaaata
     1381 atctcgatca attttttgat aaaagctaat aacaatatcg tcactttgta attcggaacg
     1441 gtgggcaata agaaatagaa gtgctttgtc agagttttct aatggaagag aaggtacagt
     1501 gctttcttgc atttcagatg ttgaagaaat gctaaacgac tgaatacttg attggctatg
     1561 ttgcgaatct acattaccag tagaagtatt agtaatggaa gtagtagaag tacttttagt
     1621 tgaaccaacc tcagttgttg aaatggctgc tgaagattct ttaacagaat tctttgtttt
     1681 atatccacag gcagaaaaaa gtattacagt tcccaacaag cctgcgtaaa tccctttatg
     1741 catgatagct cctcctaaaa tagattatct ttattcagta tatcacttaa acaaagaaaa
     1801 gcagatggtt taaaatgaaa aaaccttccc aataaaggga aggttcatga agataataac
     1861 ttttttaata attatagctt tctaatatct ttcatcaact tttctagtaa tcattgggaa
     1921 aaatttcaag cagtagatcg cacaaatacc tacgacgata taaatgatct tcgcaaaaat
     1981 agttgttgaa ccaccagcaa tcatagctac taaatcaagt tcgaacaagc ctaccaacag
     2041 ccagttcaac cctcccacaa tcaataaagt taatgcaata gcatctaagt tttcattaga
     2101 attctccttt cttaaatgat aaaacaatta tatatcaaaa agagaaacat tgcttatata
     2161 acgctcagga attttagcaa tctatgcaaa aagatgaaaa ttttttttgc tcttccatta
     2221 gtacacattt tttagctgaa gctgtaaatt tcatgtctta atttttttta gtgagctttt
     2281 atactggatt tgtaagaaaa aagaaggaga ggatcacata tgtcggattg gttggatatt
     2341 gcaggaaaag tagtaattgt aactggaggt tcctcaggaa ttggaagaag tatcgtcgaa
     2401 aatttgttga agcagaatgc acaagtagcg aattttgatg taactgaatg ccgtatacag
     2461 catgaaaacc tactatcttt aaaagtcgat gtgtcttcta aaacggacat agaagaaggg
     2521 atatataaag taatgaaaca tttcaagacg atcgatggtc tagtcaataa tgcaggaata
     2581 aatattcctt ctcttctaat agatagaaat catccaaaaa gcaagtatga attatcagaa
     2641 caagtttttg ataagatgat cgctgtcaat caaaaaagtg tatatttgat gagccaagca
     2701 gttggaagaa tcttggtgca aaagggatca ggagtgatcg tgaatctttc ttctgaatca
     2761 ggattggaag gatcagaagg acagagctgt tatgctgcta caaaagcagc gatgaacagt
     2821 tttactcgtt cttgggcaaa agagttagga aagaggaatg ttcgtgtggt aggtgttgca
     2881 ccagggatac tggaagaaac cggtttgaga acacaagaat atgaagaagc tctttcttat
     2941 acgagaggta taagcgtcga acagctgaga aacggttaca gtcgaacaag tacgatccct
     3001 ctaggaagga gtggtaagct gcaagaagtg gcggatctag tctgctatta tctttcagaa
     3061 agatcaagtt atatcactgg agtcacgact aatatttcag gaggtaaaac aagaggataa
     3121 aggatagggg ggacgcacat gacgctagta aacagatggt accaattatt gaaattacta
     3181 gtcactcaca agaaagtttc aatccaggaa gtgcaaaaag aaatcaaaac cagtaatcaa
     3241 accataaaaa aagatattgg ggagttgaat gaagaaattg ccggtattgc aaaaatcatc
     3301 caaaaaaaca aacattttga acttgaaatt tatgattttg atttttttga tgagatcatg
     3361 caaggaaaat tgaaaacagc cagtgatttc aattcggcat ccaaaagaat cgcttattta
     3421 ctaaataggc tgatcgaaac agaggatttc ttacgtatgg atgatttgtc tgaagaagtc
     3481 ggtgtcagca gaggaacagt ggtcaaggat ctaaagacat taaaagagat gatcaatgat
     3541 tttgatgtaa agattacagg cacaccaaat aaaggactaa gaataatagg agatgagcta
     3601 gaattacgac tacttttatt ttattttgtt tctccctact tttcagagac gaaaacagaa
     3661 cagtttctag aagaaaaaca agcaatcaaa cattttcaga agaagacgaa tgctagtaaa
     3721 caagaaatca cccttttaac gaaagttatc ggtatttctt tgaatagaat ctctagtaac
     3781 tatctgttga agaagcccat caatcgttac agtgggtgtt ttgaatatat ccaagaattc
     3841 aatgaactga tcaatcagtt ggaagaaatc tatgaactaa ctttgagcca atatgaaaaa
     3901 gattttatca gttttccttt gaacatcatc aacacaagta taacgatgat aaggaaagct
     3961 aactctgatt tgcgcttata tttcgacccc atgatgatgc gtattcgacg attagatctc
     4021 attgatctgg atgagtcctt cttatatcaa gagatgaaac agcacctctc tttattgata
     4081 aatcgggtaa tttttcgtta tcgattgact gatttatttt atggtgagat agaaaaaaag
     4141 tatccttttt cttaccaaat cgctagcgaa tgcatacaag cattagaagc tatcttgcaa
     4201 cataagatct ctaaagtaga gatcagctac ctaacgttgt atttggagtt agcatttcga
     4261 tatcaaaaag agcatttttc ctctaaaaaa gtagccattg ttcatacgac aggaaaagga
     4321 acagcattaa tgatccaaag acaaatcaaa aggatattgg ggagggatat acaaataaca
     4381 catgtttcag aagaacaata tgaaaaagta gatctaaaca aatatttcgc tgtttttaca
     4441 acgattccgt taaaaaatgt aaatgaagag acaccggtga tccatttgac taatcttttt
     4501 gatgactctt ggttaagggc acaatggcaa aaagcaaaaa aaaagcgatt agccgggttt
     4561 cattctactg tttttttcta tcaaatatgg aaagaaaaaa agacgtatca agaatattta
     4621 aacaaattag tcgctccttt gatcagaggc tattatgtgg atgaagaatt tgaaaaaagg
     4681 atcttgaaaa gagaatatca gcaaagcaca tttgttaaca aagaatttgc ttttcctcac
     4741 acaaccaacc agctatctca acacattgtt ttatcagtag ggctaataac tgatcaaggt
     4801 ttagcagaaa caggaggaaa tggagttatt tttttatttg ccatacctga agaaatgacg
     4861 gctgaagttg agaatgaatt atttgagcta tacgatatcc tatttgaact cataaaaatg
     4921 ggaatagatg agtttattga attagcaccg acacaagaat catttttaaa actaatcaaa
     4981 agtaaaggag agagacttca tgaatgatat gtatctattc atagttttcg cagcttcagc
     5041 ttatgtgctg caagcagtta tcagttggaa gcaactaaag catttcaatc aaacatatag
     5101 cgaactgcga agaaaaggaa aagtagcgat tggtaaacga cctgggaaaa tccgttcagg
     5161 cacgattgtt atgctagcta tagacgatca tgatcaagtt ttagaagtca gaaaaatgca
     5221 aggggttaca tctttggcaa aatttcattc attacctgtc tatgcgggtg aagatatcca
     5281 ttacatcgat cgttaccatc cctcagttcg agcggaagat cgtttgacac aagcagcttt
     5341 agaaaatgcc agagaagtat acttacgaat agaggcagga aatgaaaaaa gtgaacaacc
     5401 ttcattgcct tttattgcta tgctcaataa ttttcggata tggaaaagta cgatcaaaga
     5461 aaaaaggagt gacaaacgat ggatatcata acaaaatttg ctgagggatt tatgaggctg
     5521 ttccaaacag gtgcagaaac attcataagc tggatgacag gaatcgtacc agttgtcttg
     5581 atgctgatgg ttgcaatgaa tacgctgatt gcttttttag gtgaagaccg agtaacgaaa
     5641 gtagccaaat tatcagcgaa aaatccgctt actcgttacc ttgtgttacc gttcttatct
     5701 gcttttatgt tgggaaatcc tatgtcattt acaatggctc gtttccttcc agaatactac
     5761 aaaccaagct attatgctgc tcaagctcaa ttttgtcata cgagcaatgg tgtattcccc
     5821 catattaatc ctggagaatt gtttgtatgg atggggattg ctcaagggat cgaaacctta
     5881 gggctaaatt cgatggagct ggctatccga tacttgcttg tgggactgct gatgaacttt
     5941 attggtggtt ggataactga ttttactact ggatttgtat gcagacagca aggaatcgtt
     6001 cttagcaaaa aagtagagct ttcagttgat tagagaaagg agagttttaa atgtatggtg
     6061 atattaagat cgtaagagga aacggcgggt atggaggtcc tttgattatt aggccgacaa
     6121 aagataaaca caagttcatc tatgtcacag gtggaggtga gaggccaatg atcgtagata
     6181 aaatagaaga actgacaggt atggagtcta tcaatgggtt caaaacatca atacctgata
     6241 gtgaaatcgc gcttgcaatc gttgactgtg gagggactct tagatgtgga atttatccta
     6301 aaaaagggat accaaccatc aatatcgttc ccacaggaaa aagtggccca ttagcacaat
     6361 atatcactga agatatttat gtttcagcag tagggttaga acaaatacag ccactaatgg
     6421 aaactgatga taacaaagaa acagtaaata ctgcatctcc agataaaaag tacaaatatg
     6481 atacttctaa aaagataacc gagcaaaaag cagagcagca aaactggata gcaaaaattg
     6541 gaatgggcgc gggaaaagta gttgctacat tcaatcaatc agcaagagaa gcagtagata
     6601 cgatgatccg gacagtgatc ccttttatgg ctttcgtttc ccttttgatt ggtgtgatac
     6661 aaggttctgg gatcggtaat gtatttgcta acattatggc acctttagca ggaaatgcag
     6721 ttggattaac aatcattggc tttatttgtt cactgccatt tttatctcct ttgcttggtc
     6781 caggggcagt tattagccaa gtgattggaa cattgatcgg tgtagagatt gggaaaggaa
     6841 atattcctcc ccaattagca ttaccggcac tatttgctat caatactcaa aatgcatgtg
     6901 attttattcc agtcggtcta ggtttggaag aagcaaaagc tgagacagta gaagtaggtg
     6961 taccatcagt tctttattca agatttctta acggcatccc acgagttatc attgcttggg
     7021 tagcaagttt tggattatat agctg
//
LOCUS       DAFEOM010000105         6671 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.111, whole genome shotgun sequence.
ACCESSION   DAFEOM010000105
VERSION     DAFEOM010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.111"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            71..154
                     /locus_tag="LP524_002616"
     CDS             71..154
                     /locus_tag="LP524_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBL2235613.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            279..1016
                     /gene="erm(B)"
                     /locus_tag="LP524_002617"
     CDS             279..1016
                     /gene="erm(B)"
                     /locus_tag="LP524_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBL2235614.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1186..1446
                     /locus_tag="LP524_002618"
                     /pseudo
     CDS             1186..1446
                     /locus_tag="LP524_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1609..2274
                     /locus_tag="LP524_002619"
                     /pseudo
     CDS             1609..2274
                     /locus_tag="LP524_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2310..2555
                     /locus_tag="LP524_002620"
     CDS             2310..2555
                     /locus_tag="LP524_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL2235615.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2658..3527
                     /locus_tag="LP524_002621"
     CDS             2658..3527
                     /locus_tag="LP524_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBL2235616.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3508..4242
                     /locus_tag="LP524_002622"
     CDS             3508..4242
                     /locus_tag="LP524_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL2235617.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4275..5190
                     /gene="ant(6)"
                     /locus_tag="LP524_002623"
                     /pseudo
     CDS             4275..5190
                     /gene="ant(6)"
                     /locus_tag="LP524_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            5187..5729
                     /gene="sat4"
                     /locus_tag="LP524_002624"
     CDS             5187..5729
                     /gene="sat4"
                     /locus_tag="LP524_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HBL2235618.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5822..6616
                     /locus_tag="LP524_002625"
                     /note="aph(3')-IIIa"
     CDS             5822..6616
                     /locus_tag="LP524_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HBL2235619.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT         2340 a         1003 c         1393 g         1935 t
ORIGIN      
        1 ttttgtgtat aataggaatt gaagttaaat tagatgctaa aaatttgtaa ttaagaagga
       61 gggattcgtc atgttggtat tccaaatgcg taatgtagat aaaacatcta ctgttttgaa
      121 acagactaaa aacagtgatt acgcagataa ataaatacgt tagattaatt cctaccagtg
      181 actaatctta tgacttttta aacagataac taaaattaca aacaaatcgt ttaacttctg
      241 tatttattta cagatgtaat aacttcagga gtgattacat gaacaaaaat ataaaatatt
      301 ctcaaaactt tttaacgagt gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa
      361 aagaaaccga taccgtttac gaaattggaa caggtaaagg gcatttaacg acgaaactgg
      421 ctaaaataag taaacaggta acgtctattg aattagacag tcatctattc aacttatcgt
      481 cagaaaaatt aaaactgaac attcgtgtca ctttaattca ccaagatatt ctacagtttc
      541 aattccctaa caaacagagg tataaaattg ttgggagtat tccttaccat ttaagcacac
      601 aaattattaa aaaagtggtt tttgaaagcc atgcgtctga catctatctg attgttgaag
      661 aaggattcta caagcgtacc ttggatattc accgaacact agggttgctc ttgcacactc
      721 aagtctcgat tcagcaattg cttaagctgc cagcggaatg ctttcatcct aaaccaaaag
      781 taaacagtgt cttaataaaa cttacccgcc ataccacaga tgttccagat aaatattgga
      841 agctatatac gtactttgtt tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta
      901 aaaatcagtt tcatcaagca atgaaacacg ccaaagtaaa caatttaagt accgttactt
      961 atgagcaagt attgtctatt tttaatagtt atctattatt taacgggagg aaataattct
     1021 atgagtcgct tttttaaatt tggaaagtta cacgttacta aagggaatgg agataaatta
     1081 ttagatatac tactgacagc ttccaagaag ctaaagaggt ccctagcgcc tacggggaat
     1141 ttgtatcgat aaggggtaca aattcccact aagcgctcgg gaccccttgt aggaaaatgt
     1201 cctaagtgtg gcaacaatat tgtattaaaa aaatcgtttt atggttgttc aaattatcct
     1261 gaatgtaagt ttactttagc tgaacatttt agaaagaaaa aactaaccaa aacgaatgta
     1321 aaagaattac tggagggaaa agaaaccctg gtaaaaggaa tcaaaaacaa agagaaaaag
     1381 ccctacaatg ccgttgtaaa aattggggaa aagggatata ttgattttat ctctttttca
     1441 aaataaacat ggggctgacc caaaagtagc tcctatatga aaaatagagg tctttccatt
     1501 tagatagtag tggaaagacc tctatttatc ttttgaaagt gaatacaaaa ataaagcaca
     1561 ctctgctata atataggtga ttaatccatt tgagagaggt gctttatcat gtatcaaaat
     1621 tataccataa accaactttg tttgcctata gatcttgaaa ttaaattaga agaaaatgat
     1681 tttgctcatg ccattgtgca gtttgttgac tctattcccg atgaagtctt tcttccttat
     1741 tatcaatcta tgggtagacc ccaatatcat cctcgtatgc tactttccat tattctttgt
     1801 gcctatattc aaggggttta ttcgggccgc caaatacaaa atatgctgat agatagtatt
     1861 cggatgcgct acttatctca agagcaattc cctaactttc gtactattaa tcgttttcgc
     1921 gttcatccga ttatgaacga tattctagat cattcatttg ttcaatttcg tgagctactg
     1981 gttcaaagtg gcttaatcag tggcagtgct ctatacattg atggtacaaa aattgaagcg
     2041 gatgcgaata aatactcttt cgtttggaaa aaaagtattt taaagtataa ggatagcctt
     2101 gatcaaaaag cccttgaaaa ctaccaacaa atggtggaaa caaaaatctt acctgagtta
     2161 atagatgatt taaaggatga actttctata gaagagattg aaaggattcg tcaatctttg
     2221 gaagaaaagg aaaatcaatt aattgaagcg attgaccaaa ccgaaacagt agaagggggc
     2281 tgacccatta ttaataagga gtggacaaca tgattgatgt aaatgagcta ttggaagaag
     2341 ctattagaga aactgaaaat ctaaatgatg gcgaagtttt tcttgttaaa gatttattca
     2401 agggatatgt ggggaatagg atacccagaa aggatcgact tctgcttgga acgttatttt
     2461 taaaccatgt tagcaaaatg aatggtaatt taaaggctat tgaaaagact tcatctaatc
     2521 agcagaggta taaaaagaca attgataagt tttaactatc ttttgtgaaa ttaaaaaaat
     2581 tataaatatg ttgagtagaa gcaaaagatt tggcttggaa tttttacagc agtgaatcga
     2641 taaaaggagc atgcttgatg gtagataata ttattaaatc agtagcagag aaattatcct
     2701 ctctgtctta tatagaaggt attgttttag gtggttcacg tgcaaggggc acccatacag
     2761 aggattcgga tatagatatc ggcatctatt acaattcaga atcatttgac ataaatacta
     2821 ttaatcaatt cgctacaaag ctggatgatg agcatagaaa taaccttgtt gtacctcccg
     2881 gagcatgggg tgattggatt aatggcggcg gatggttagt cataaacggg tatcatgtgg
     2941 atttaatttt acgtgatatt aaacgtgtgg aacaaataat gaaagataca gagcacggaa
     3001 ttgttactgc caattatcag actgggcatc cccatggtta tattagtgca atgtatcgag
     3061 gagaattagc gattagcaaa atactatatg ctaagaatga aagcttatgc gaattaaaaa
     3121 aacaggcaga aacttatccc aatgctttgc agaaaagttt agttaacttt tttatgtttg
     3181 aagcagggtt ctctttaatg tttgtaaaag caaattcggg aacagacgat aaatattata
     3241 ttgcgggtca tgtttttcgt atagtttcat gtttaaatca agtgttattt gcatgtaata
     3301 atgcttattg tatcaacgaa aagaaagcta taaaactgct tgaaactttt gaacataaac
     3361 ctgaaaaata taccgagaag gtaaatcata tttttgaagt actcggtatc tcactttttg
     3421 aatgctacga catgaccgag aagctttata atgaagtgaa tgaaattgta tcggagataa
     3481 ataacttttt aaacgaggag agttcagatg aaagaaaaca aatatgatga taatatattt
     3541 tttcaaaaat acagtcaaat gagtcgctcg cagaaaggac tggctggtgc gggagaatgg
     3601 gagactttga aaaagatgct acctgatttt aagggtaagc gtgtgcttga tttaggatgc
     3661 ggctatggat ggcactgtat atatgcgatg gaaaacggtg cttcctctgt agtaggtgtt
     3721 gatatttctc ataaaatgct cgaagtagca aaaggaaaaa cccattttcc acagattgaa
     3781 tatgaatgct gtgccataga agatgtggat ttcccagagg agagctttga tgtaatacta
     3841 agttcgcttg cgtttcatta tgtagcagac tatgagaatt taataaaaaa gatatatagg
     3901 atgctgaagg ctggtggcaa tttagttttt acagttgaac atcctgtttt tactgctcat
     3961 ggaacacaag actggtatta taacgaaaaa ggagaaatac tgcatttccc ggtggacaat
     4021 tattattatg agggcaaacg gacagctatg tttttggaag aaaaggttac aaaatatcat
     4081 agaacactga ccacatatct aaatacactg ctttcaaata gttttataat aaatcagatt
     4141 gtggagccac agccgccaga gaacatgatg gatattccgg ggatggcgga tgaaatgcga
     4201 cgcccaatga tgctgattgt atcggcaaaa aagaagatgt aataatatag aaaaaataaa
     4261 cgaggagtat gtaaatgaga tcagaaaaag aaatgatgga tttagtactt tctttagcag
     4321 aacaggatga acgtattcga attgtgaccc ttgaggggtc acgcgcaaat attaatatac
     4381 ctaaagatga atttcaggat tatgatatta catattttgt aagtgatata gaacctagaa
     4441 ccgtttatat ctaatgatga ctggcttaat caatttggga atataataat gatgcaaaag
     4501 ccggaggata tggaattatt cccacctgaa gaaaagggat tttcctatct tatgctattt
     4561 gatgattaca ataaaattga tcttacctta ttgcccttgg aagagttaga taattaccta
     4621 aagggcgata aattaataaa ggttctaatt gataaagatt gtagaattaa aagggacata
     4681 gttccgactg atatagatta tcatgtaaga aagccaagcg caagggagta tgatgattgc
     4741 tgcaatgaat tttggaatgt aacaccttat gttattaaag gattgtgccg taaggaaatt
     4801 ttatttgcta ttgatcattt taatcagatt gttcgccatg agctgctgag aatgatatca
     4861 tggaaggtcg gcatcgaaac aggctttaaa ttaagtgtag gcaagaacta taagtttatt
     4921 gaaaggtata tatccgagga tttgtgggag aaacttttgt ccacctaccg gatggattcc
     4981 tatgaaaaca tatgggaagc attatttcta tgccatcaat tgttcagggc ggtatccggt
     5041 gaggtggcgg aaaggcttca ttatgcctat ccggagtatg ataggaatat aacaaaatat
     5101 accagggaca tgtataaaaa atacactggt aaaaccggct gcctggatag cacatatgcc
     5161 gctgatatag aagagaggcg ggaacagtga ttacagaaat gaaagcaggg cacctgaaag
     5221 atatcgataa acccagcgaa ccatttgagg tgataggtaa gattataccg aggtatgaaa
     5281 acgagaattg gacctttaca gaattactct atgaagcgcc atatttaaaa agctaccaag
     5341 acgaagagga tgaagaggat gaggaggcag attgccttga atatattgac aatactgata
     5401 agataatata tctttactac caagacgata aatgcgtcgg aaaagttaaa ctgcgaaaaa
     5461 attggaaccg gtacgcttat atagaagata tcgccgtatg taaggatttc agggggcaag
     5521 gcataggcag cgcgcttatc aatatatcta tagaatgggc aaagcataaa aacttgcatg
     5581 gactaatgct tgaaacccag gacaataacc ttatagcttg taaattctat cataattgtg
     5641 gtttcaaaat cggctccgtc gatactatgt tatacgccaa ctttgaaaac aactttgaaa
     5701 aagctgtttt ctggtattta aggttttaga atgcaaggaa cagtgaattg gagttcgtct
     5761 tgttataatt agcttcttgg ggtatcttta aatactgtag aaaagaggaa ggaaataata
     5821 aatggctaaa atgagaatat caccggaatt gaaaaaactg atcgaaaaat accgctgcgt
     5881 aaaagatacg gaaggaatgt ctcctgctaa ggtatataag ctggtgggag aaaatgaaaa
     5941 cctatattta aaaatgacgg acagccggta taaagggacc acctatgatg tggaacggga
     6001 aaaggacatg atgctatggc tggaaggaaa gctgcctgtt ccaaaggtcc tgcactttga
     6061 acggcatgat ggctggagca atctgctcat gagtgaggcc gatggcgtcc tttgctcgga
     6121 agagtatgaa gatgaacaaa gccctgaaaa gattatcgag ctgtatgcgg agtgcatcag
     6181 gctctttcac tccatcgaca tatcggattg tccctatacg aatagcttag acagccgatt
     6241 agccgaattg gattacttac tgaataacga tctggccgat gtggattgcg aaaactggga
     6301 agaagacact ccatttaaag atccgcgcga gctgtatgat tttttaaaga cggaaaagcc
     6361 cgaagaggaa cttgtctttt cccacggcga cctgggagac agcaacatct ttgtgaaaga
     6421 tggcaaagta agtggcttta ttgatcttgg gagaagcggc agggcggaca agtggtatga
     6481 cattgccttc tgcgtccggt cgatcaggga ggatatcggg gaagaacagt atgtcgagct
     6541 attttttgac ttactgggga tcaagcctga ttgggagaaa ataaaatatt atattttact
     6601 ggatgaattg ttttagtacc tagatttaga tgtctaaaaa gctttaacta caagtttttt
     6661 agacatctaa t
//
LOCUS       DAFEOM010000106         6644 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.112, whole genome shotgun sequence.
ACCESSION   DAFEOM010000106
VERSION     DAFEOM010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.112"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(1..859)
                     /locus_tag="LP524_002626"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="LP524_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="LP524_002627"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="LP524_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBL2235620.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="LP524_002628"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="LP524_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="LP524_002629"
     CDS             complement(2143..2373)
                     /locus_tag="LP524_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HBL2235621.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="LP524_002630"
     CDS             complement(2603..3421)
                     /locus_tag="LP524_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL2235622.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="LP524_002631"
     CDS             3582..4271
                     /locus_tag="LP524_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBL2235623.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="LP524_002632"
     CDS             4285..5787
                     /locus_tag="LP524_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL2235624.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="LP524_002633"
     CDS             5800..6276
                     /locus_tag="LP524_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL2235625.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="LP524_002634"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="LP524_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2236 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DAFEOM010000107         6631 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.113, whole genome shotgun sequence.
ACCESSION   DAFEOM010000107
VERSION     DAFEOM010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.113"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(<1..1118)
                     /locus_tag="LP524_002635"
     CDS             complement(<1..1118)
                     /locus_tag="LP524_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBL2235626.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDG"
     gene            complement(1189..1533)
                     /locus_tag="LP524_002636"
     CDS             complement(1189..1533)
                     /locus_tag="LP524_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HBL2235627.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(1543..1917)
                     /locus_tag="LP524_002637"
     CDS             complement(1543..1917)
                     /locus_tag="LP524_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL2235628.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(1930..2244)
                     /locus_tag="LP524_002638"
     CDS             complement(1930..2244)
                     /locus_tag="LP524_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL2235629.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(2358..5585)
                     /gene="fss3"
                     /locus_tag="LP524_002639"
     CDS             complement(2358..5585)
                     /gene="fss3"
                     /locus_tag="LP524_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HBL2235630.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            complement(5664..6326)
                     /locus_tag="LP524_002640"
     CDS             complement(5664..6326)
                     /locus_tag="LP524_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235631.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
BASE COUNT         1771 a         1424 c         1122 g         2314 t
ORIGIN      
        1 atcccgtctc ctaaatattt cgcatccggt ctttgacaag ctagaatcag aaagaaacca
       61 gattgacgac ctaacatcac gatttgtttg agcttattca aaatcaccgc actttccttc
      121 gtcgttaaca tttccatata aaccacgtat tcatcaaaga ttaaaaagtt tggtggaagt
      181 ccaagaaacg cataattctc tcctgttttg tagttgggca tttctttcat tgccttgcta
      241 cgagtcatca tgcgctcata gaaatcttcc acacaagcag aaatttcctc cttttgggaa
      301 tagacatgag gcattaccgt acctaaatcg gctaagtccg catttttggg atcaagaaca
      361 aaaagctcgg catcagattt taataaagcc tcgatgatag tgagtaaaaa gtaagtcttc
      421 ccaccacctg tgccgccagc gattaacata tgaggtaaag aatcataagc ccatacttga
      481 tttttcatca atcgcaaagc cccactttct gccactactt cgtctatccc aattcgattg
      541 gcaattatat catacagtaa agtgtactcc acataggagt ctttgagttc cttttctact
      601 agttcacaat atagaccact ttctaacttc ttttccagct ttaaaagctg atcttgatat
      661 tttcccagtg aaatttgaac gcgaatagaa agtaaaccat ccttcatacg gtaatagatt
      721 ttggggaagt aactgattgt ctctttggtt cgactactat tgaggtcttt gaaaaatcct
      781 tcactctgag tttgttttac ttcgtaccaa tgattttcta gaatcatccg ggctagcttt
      841 tgtcgatgcc acaaactgcg ccaatgatcc tgaaaaaagt gtatataaag caacatcgtg
      901 cttcctgaaa gaacgagact tcctagcaaa tagattaaat cttttataga acgaacgatt
      961 tctatagtag aaagtgatag cttagaaata tcaaattgtc taagctcttg aaaataaaag
     1021 acgctcatcc aaccgatgaa cagagcgagt agccagccaa gaacaaagtg ataaaccaag
     1081 tgctgatctc ctgcccgtat acgatgccct ttgtacattt ttatggtttc cacctccaat
     1141 cgaaaaaagc ttcataggat ttcattgatc caatttcaag cagttatgct agtcatttac
     1201 tgctataggg agttgtttcg tttccactag ttgctttaaa aaaacttcaa tggcatccgg
     1261 catttccaca ccaatttctt ccaatacggt ttcggcttct cgacatagac tttcatttac
     1321 ttcgataacc ataaagaagg actcttcttc ccctaaccaa ttcagtacat caaaatcttc
     1381 ttcggcatcg ttacctactt ccagtaaccc ttgatgcata atcagaaaca ttttttcatc
     1441 ataggttaat gctttagtga gttgttcctc cgttagctca ccttttccat attgatcgag
     1501 acaatctaaa atatgttcaa cattaatttc catacgcttc acctatttct ttccttcatt
     1561 ttggtttgta gaatttccct gatctggttg tcgtttcaag actaaatcat cggccttaat
     1621 gaaccaattg acagtagttc cttctcggaa aacataattg gcgaccgtat ccatgattgg
     1681 attgaccaac tctacctctg cgtcataggc aaactcgcgt aagggtacat ttgctgggat
     1741 actaacttgg atcattcgac cttgcgcaga ggatttcaaa ttataagtcc gttcttttac
     1801 ctcgttacta accgttccat cctcattttg cttccgtact tctcggcgta atgcggaaaa
     1861 tcgtaatttc ccaaacgtta gtcctgaatc tacaacaata ccttctgtta atctcatctt
     1921 cttttcctcc tatgctttca ctaaatcatc gacatacaac acgtagtcgg taaacccacg
     1981 attctcaatc tcatagcctt tggcaaccaa atggggattc acggctttta gtggttgatc
     2041 ttgatcaaaa tccttttcgc cggcttccgc agctaccacc acttcaatat catcggctcg
     2101 ttgcttactg gaatacaaat gataactacg accaattact gtagtattcc gcccatatcc
     2161 ttcagttaaa acttcgcctt cgccggcata ccttatcttt ccaaaggttt cggccatatc
     2221 tggcacaaca aatttcaatt ccatcttctt ttcctctttt catttttagt tttggataca
     2281 aaaatagcgc gaccactttt tttaggaggc ggtcgcgcac gttggttttt cctatttagt
     2341 tgtttcatca catcacctta gtttcggcga ttccagaaat aatacccagc agtcgcaaag
     2401 atcaaggtaa acccaataaa cagcagaaca ttagaatttt tctcccctgt ttgtgggaat
     2461 gtctttactg taggactaga ttcttgagag tcacttggaa cagttggtat ttccggttgt
     2521 tctggtgcac ttggtgttgt cggaactttt ttaggtgtta aagtttgagt tttttctttt
     2581 agatcctcat tgtgtttccc attgatgttt ccgtctttgc catagttaat ttctttgaag
     2641 gtaaaacttg taccttctgg atatttactt gtgtccactt tttccgcaag tacggtttta
     2701 gtgaattctt tgtcattcac ttcataatca attttgccag atttccaaat ttctttggtc
     2761 gtattatccg gaagtaatgc atacagaatt gtttcaaaag cttcttttga gccatccagt
     2821 acatcatgag taatcgacac atcatcgaac atatccacca catccccatg agtaaaggtt
     2881 tgcgaaccat cttctaggtg ggcttttgtt tggatggaaa catggcgttc caccgtacaa
     2941 ttgacagttt gggcttgatt gttcaagcta gcatctttcg ctactggatc atcacctgct
     3001 tcatagggtt tcttgttttc atacacatag ttgaataaga cgatggtttt atccaaaact
     3061 tgttctgcgg ttaatttatg caaaattttc caagttcctg ttttctcttt attcgccaac
     3121 gttgcggttg tttcggcaat taccactggt ttggcttttt catctttttc ttgggcagct
     3181 tttgtcgctt ccacatcaat ggcttgcgct accacatacc agtcttcttt gatttcatgc
     3241 aagttatagc tcaatttatc aactagctcg gtagaatcaa ggtctgtcaa ttctttgcct
     3301 ccgtctttcc aagtcccaag agaagttaag ctatcttctt cttcaggtaa atcatagagc
     3361 atcaaacgat tcaagctaac agaaaatgct ttattcgtca gtttctcgta aggaacggtc
     3421 accgttttaa ttggttgttt ttgtcctttt tcggtaatcg taaaaatgta ttcactctta
     3481 gtgtaatccg ctttattttc tttaaaagta gaacggattt ctaatggcgt aattttttga
     3541 aatccttctg gagcctctac ttcttcaagt aaataatccc cataaggaag attttcaaac
     3601 ttgccatagc catcaaaacc tagttgctcg ttataagctg taattgcttc atcttcggca
     3661 cctgtaatct cactggttcc ttccaatggc gatactttaa aggtcagatc attgaagcca
     3721 gtttcggcag tcccagcagc tgatccagca aatttaaaga aatcaaagcc aaagcgaatc
     3781 acttgttctt ttgccgcaac atctcgagta atcacagcat ttttttcgct atcatccacc
     3841 tttttgatgg atacaggata ctttgtttca tccaaggtat atccttcagg cgctttggtt
     3901 tcttgccagt aatactcatt aatcgctaag tgtttaacgg caacttgatt cttttcatct
     3961 aaagtcaaag ttacggtttc atcagaagct ttcgttccct tcaacaattc tggtttaaag
     4021 ttctcggtcc atttgaccgc ttttccatcc ttaacagtaa acaaggtgta ttcagctctt
     4081 ttaaactcag ctttcccttg agtttcatga ccggtatctt tgtcttcttt tgtcaaagtg
     4141 gtttcaccag taatttcttg gttttgccct tttacgttac tggtaacaag agccacggtt
     4201 tgattgacat aattcaattc gacttttact ggtttaaagg tattcacaaa gccattacta
     4261 gctttggttt cggtcacata ataggttccc aattccaacg cattggcaat ctcttttggt
     4321 gtttccgcat gacctttttc atccgtggtc atttcttgga caatttcacc ggttggactg
     4381 tctttgcgaa tggcaaacgt atttccggct aatgagtatt tgtcgttcca aagatcagtt
     4441 cctgtttcca cccccatttt attcaaaatg atttgacctt tttcgcgagt attttttgaa
     4501 gtcacagaaa tagtctcacc agatttaatg gttgcggtga ttggtgtagt gtcaatcgta
     4561 taaggagctg gcactgattt ttcagtaatg gttactttcg cgccatgagg aatctcatcc
     4621 aatgtcgcaa tgccttcttt atcggtggtc acgtcttttg caggtaatgt ttttccaaag
     4681 tctaaatgga aaacggttcc cggtacaaca tttcctgttt ctttatcgac ttttttaatc
     4741 ttcaaagaac cctcagtttc cacatcaatt ttgattgtgt aggtattggg cttatcaatt
     4801 gcccccgcca tcaaagtttg ttgaccagcc attttataag ctaccggtgt tcctgtatta
     4861 gctgacttct ttagggttaa ttcaccactc tcttttgaat tggcatttgg agtaatcact
     4921 agttgattgc cgttcacacg ataatcaata tttgcgatat tctcgacaac ttcatcaaac
     4981 tccgataaat tcaagccgtt cgtatctgtc acagtagtcg attgacctaa cacggttttc
     5041 actgtggagt tatggaagct tggtttcttt tgataatccg caatggcttg attgattttt
     5101 gactcaatgg ctgcgatatt tactgcacta ccatcagaac gtttaattga gtgaagcgtg
     5161 tagccattaa cttcttgcca aatcatcttc tgggccacaa tatgtgtatc cacatccgta
     5221 ccagcttcct tccataaaac ggaaactaat ttcgctttct ccgacatatc aggcaatgga
     5281 tttttctcat aaccaggagt gaactcattg taaatcggca cccctggctc aatacagaaa
     5341 atatcttgct tcacaccatt gtaaactgca tagtgcggaa cggtaacttc tgacatggga
     5401 cgaccatcgc taaatgtacc ttcgacagca tactggtgag ccaaatccaa ttccacagtt
     5461 accgtttgtg gatgagtaat ttcatcagca aaggcaatcg ttgggccaag cgatccacca
     5521 attgtttggc ataatagact cagtgttgcg agcaaacgcc aattcttaaa tcttggcttt
     5581 ttcataaaat aaatctcctt tcgtttttgg gtaataaaaa agacgtccaa attttaattt
     5641 tgaacgtctg atttagagtg atactattta gttttctttg tcttggatag gttgtttctc
     5701 tgcttataaa ttttgattct tcgataatca aatccaatct ctgccatcaa ttcctcccaa
     5761 tttttgttcg ttcttttcat aattgtatca actgatggta gttttgggga ttgaactagt
     5821 tttcgaaact ttgtcattga taaatcatcc tgtagtttta atcggattat ttcatttttt
     5881 agttctaaca gcagttcgaa atcagtaaat cgagatccat catttttttc tttaaaaagt
     5941 ggacgtatat ctcctaacat ctttttaatg gtactaagtg aaggaatatt cttccctact
     6001 gatttttgtt cgtacattcg ttgagaggtt atcttctcgc acttaataaa ggcttctact
     6061 atttccaata attcttgttc tgacattcta gcccattttc catacccagt attttctcgt
     6121 cctatactaa tcaataaatt ctcccatgat ccatattttt tgttaatgaa ccacatactt
     6181 ggtgcagaat ttggattttc tgatagcttt ttctggtatt gacggctaga tgtaattccg
     6241 tgtttttcga tgaattcatt aattgttaaa atcagtttta actcactttg atttttccaa
     6301 atgatacgtc catcattttg cttcacgata aaaactccta atagttattt agatagaagc
     6361 ctatcacatc aactttttag tagaaaagta catgttttgt cttttttgct aatagctgac
     6421 cctttgagaa acttgggttc aaaagttgct aaattcggta aatgttttgg tatttttcgt
     6481 attatatttg tcgtcaagct gactactaaa ttttgcccta ttttgacgac actgatagtc
     6541 aatgtcggac aatctaggac aatttttaag ggaatatgat tgaaaaatgg tccaaaacag
     6601 ccaaaaatgg cttaaaactg tgatagagaa a
//
LOCUS       DAFEOM010000108         6603 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.114, whole genome shotgun sequence.
ACCESSION   DAFEOM010000108
VERSION     DAFEOM010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.114"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(1237..1902)
                     /locus_tag="LP524_002641"
     CDS             complement(1237..1902)
                     /locus_tag="LP524_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2235632.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2082..4064
                     /locus_tag="LP524_002642"
     CDS             2082..4064
                     /locus_tag="LP524_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBL2235633.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4068..4706
                     /locus_tag="LP524_002643"
     CDS             4068..4706
                     /locus_tag="LP524_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2235634.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4734..5162
                     /locus_tag="LP524_002644"
     CDS             4734..5162
                     /locus_tag="LP524_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235635.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5619..6530
                     /locus_tag="LP524_002645"
     CDS             5619..6530
                     /locus_tag="LP524_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL2235636.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADNGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         2275 a          998 c         1213 g         2117 t
ORIGIN      
        1 actacctgta ttagtcctct tttttgacca gattttgacc agtctatttt acattctaat
       61 atattcatat gagctaattt tatgattaaa tggtaagtgt ttccttgttt gttttagttt
      121 aaactagtgt gtaatttaaa aatctatatg gaagggtctg tggggtaata cttaaataaa
      181 ggctaggaac ctgataacat caaggttcct agccttttta taataaaatg aagcgaaaat
      241 aatttctttt aaagagccga gattatcagc tatataagag tgaatttgat gcaaaaaaca
      301 gagccgaaac agtcttggtg ttcgtagctc tgcttatatg tttcgaattt aagacaaaga
      361 gttggcaaaa gatgttgata aggaagataa gataactgtg ttatataaac taggtaacta
      421 actgtaacaa gctaatttta agagattctt attagaagac gtttaggaat ataactaaga
      481 aaatgattat acaggtaatt gtcatttcaa taatgttttc taattttcgc gtctttgttt
      541 taagaaaaag gccagaatca gttggaaaaa agttcgtacg atagtgaaga ggggcaaaat
      601 ttcctactaa tattcccact aacaagagat aaaacggcca ggttggtgtg attcctctat
      661 aaaaaaatgc ccatgctacc gaaatcccat agattaacaa ctgaacaatt tttacaatga
      721 aaaaattgtt ctgccattac tcaaagtctc ttcctccttg tatcgtttat ttgtttcatt
      781 ttatcattat gataggcgct tgaacaatcc attttgtatt ttattgagta ctcttggata
      841 aactaccagc gattatcata tttatttatg ttactgtaag ccatcatata aaagtaaatg
      901 agcagatcat agaaatataa aaaagaacta aaattgatct aaattatgtg cagcttgact
      961 acgtaccaat ctatattact tactagattg gtcgttaatc tattcggatc gaatgagcaa
     1021 aatatttttg tgaatgtcca tccttgatta tgattccaaa aagaatcatt gggaggctgt
     1081 ggcgtaaata ttatactata agccagaaat acctatcatt ctgaactttt ttatttagga
     1141 actttcatct caaggctttt tcaattaatt tgtacccctg ttttttggat aagatcatgt
     1201 gcattgattt tttctatctc tacgttcatc tcctacttaa tgcctatctg gctcctgagg
     1261 gcttcttttt aaggcgtcgt catagtcttg gctgaggatt tcttggtaat ctgagggaat
     1321 ggcttcccaa gtgagcaggc gaacgttcat gacgtgtttt cctttgtgtt tgattacagc
     1381 atggtttttc tctttttcta cctctccagc gtgaaaagac agccatgtac catggccaaa
     1441 gatatcatta catacccaac gttcgatgga attgtccacg tatcctacag ggagtgtggt
     1501 atagccgatt tgtggtttca aaaaaggatt gaatcgatcg atcgtttcat ttttgtattg
     1561 ataattgata agaaaaagat agaccaaaca aaagacgagc agaaagaaag atagtgtctt
     1621 tttgagtgag aagggttgtt ttgatgaata agatttcttg ttaacaagag gttcatctgg
     1681 caatgttggt tctattgatt cggtagctga ttcaatagaa ggaaaatcaa tcggcagacc
     1741 taaagtatga tgtaaaagtg acagcatttc aaggtctggc attgtacgag aattttccca
     1801 actagaaatc gttttatcgg aaacaccaat tttttcgcct aattcttttt gcgtaagctt
     1861 acaatttctg cggtaagttc gtattttttc tccggtgttc atcttcttct cctttcgatt
     1921 ctacagtata tagatttatt ctacatgacc tatacacttg atagatacgt ttttttgatg
     1981 aaaaacggta tgattttatc aaagaatcat ttaagtaagg gagagtggtg ataatcaaaa
     2041 aaaagttcac agttttactc acaatcatag tgaccggtat catgagtggt tttgccttgg
     2101 ttagtataaa taaacaggta ctctatcgga caaatgaatt tttccatcaa acagatactt
     2161 atactccgat tcgattgcca caacatttta ttgggcaaga agaatatgag cagctaatca
     2221 tggtatttga aaatgtctca tcagaaactg agctttctta tatgaaaaaa acagtaaatg
     2281 aatccaatga agtagataca tcttggaact tcaagataca gccaaagctt gaaatgacat
     2341 tgtattatca aggggatgtc aaatcttctc acgagaaggt accctattct aatacctcct
     2401 ttcgcgtaac gtccattgaa gaatcgatca aaataaaaga ttttgaaggt gaatttttct
     2461 tgatgacgag agaaaaagaa gcctatgaac ggttcatttc actattcact gatcgattca
     2521 atcaagcatt tcacactgat tatgccacta atatttttga agactttgaa aatagctatt
     2581 cgtattcggt tatttcaaat acagacgtat ataattatcg agtgtattta actattggat
     2641 tgattctttt attccttttg ttatcatcct ttttcttatt gatgaataaa gaaatcagtt
     2701 tattgttgaa taatgacttt tcattacatc aagtattgtg gtgtttattg gggaaaaaga
     2761 aaatcattat tacattcata attgcaggaa tcattggtgg ttttattttt atgtattata
     2821 aggagcaatt tctttatttg ttccagatgt acgttttctt gttcagcgga atatatggta
     2881 tctctttgat ttctctttta ttagttgaaa aaatcgcttc gttaagagaa gcgttcaact
     2941 atatccaata cattcttttt acatttggat tgttgttttc tcttcttttg accgttgcca
     3001 atgtcgaatt agcaactatc gttttttctt cttcacagtt agctgtttcc catgaaccac
     3061 caaaagaaat cgctggtgag gactatcatg tattttatcc agtaaccatc gggaaaaacc
     3121 acgtggattt tatttattcc aatcgttttg catcagcagc agatcaggaa ttatatgaaa
     3181 ctttgaataa aaacggaagt atattagtca atgtatcaga ttacttgaat gaagaaaatg
     3241 aacagtttgg cagagggata aaaataaatc tgaattatct taagaaattt cctttgattg
     3301 atgtctctgg aaggttgatc caaataaccg aaaaagaaac gcaccctgtt atattgatcc
     3361 ctgaacgata tagacataca gatttaaaaa atgacctggc gcaaataaca gaatattatc
     3421 aagaaatcag tgaaaaagaa ccgaaatttc tatttataaa aaacaatcaa cccatttata
     3481 catttattcc ttctatcccc tggatcgaac attatccggt tgttgaaata ctgacattga
     3541 aaaacagtat gtatttggaa agaaatattc tcagcggaga aatctatcca ccgttaaaga
     3601 tcaagatagg ctctttgtcg aaagaaaggt tatttgagct gattgaagaa agtcagttac
     3661 gtgataactt gcagttatca tttccgtata ctcagactga agagatagct gtcaaagtgt
     3721 taagcagatc atttcattac ttgatacaga tatttttact cactttcttt tctttcttct
     3781 tgttgagcta tgtcatgtta tgcatatatt ttgtctacaa ttgtcggaaa atcgctatct
     3841 ttcgttcaag tggttattcg ctctttgaaa cgtataaaga tttcttcatg atgaacctca
     3901 tcaaatgggg aacgacaagt gtaatcttcc tttttcttat agaaagagag cctaaatatc
     3961 tttttaatat ttttttcttt agcttcattg attttattct ttcagttgtt tttattttgt
     4021 ctacagaaaa gaaaagtcag ttattgctga tgaatggagg ttaacaattg gagccgttga
     4081 tagtgataaa aggtgcgaaa aaaatcattc gaaacaaaac gattcttcat ttggatcgtt
     4141 tcactgcgga tgctggggat cttgtgactg ttgttggaga aagtggagca ggtaaaagta
     4201 cgttattgaa tatattagga ttgaatgatc agttcagttc aggacaattc tatttgttcg
     4261 atcaagagac gaaacaatta actgggagag agaagcgctt gataaaaaga aataccatat
     4321 cttttctttt tcaagatttt gggttgattg aatatgaaac aatcgatttt aatttagata
     4381 ttggattgaa gttttcgaat ttaaatgttt cagataagcg aacagcaaag tatgaggcat
     4441 tgaaaaaagt aaatataaac aagaagctaa gtacgcctat ctacacatta tctggtggtg
     4501 agaagcaaag aatcgcctgt gcgagagtct tattgaagaa tagcaaaatc attttagccg
     4561 atgaaccgac tgggtcattg gataaaagaa ataaggataa agtagttgaa atcttatcgc
     4621 aacaagcttc ccaaggtacg ttagttatcg tagtcacgca tgatccatat ctgaccaaaa
     4681 taggaaacaa gaagatcatg ctttaaaaat caaaaaggaa aggatgtcgt tttatgaaaa
     4741 aagcagttat tatttgtgca ctagggatag gttttgttgg aggaaacgct atgtcatctg
     4801 aagtggaagc aaattcagtt cacggtgaaa tctattacaa atctttaaag ccaggagaga
     4861 ttcattttga tgctaaaggt aatattatta agtcaaattt accagtagac cgggaaattt
     4921 ctagaacagc aattaatcat agctctggaa gatgggttta ttatagtgat acaacttgta
     4981 cgagaaaaac aggtcattct aaccactata gtcgtgtcta taatcgtcac ggtgcaaaag
     5041 cgaaagttgg aaactctatt cgatcagcaa atgcaacaaa aggagtatgg gcctatgcaa
     5101 gtgcgacagg aaatagaact gacacattct catgttggta caatccaacc ggttggtatt
     5161 aagtatccaa aagtttatat aaaatagaaa aaacatataa tgaaacagcg ttaaaagtta
     5221 tttttggtag ttttaatata ttcattttta gttgtataaa aatatatcat aataaaaaca
     5281 acaaaaaaat gtctggaaaa tcgaacataa aagtgtctga tttgaatatc ccagcagcgg
     5341 aagaagaaaa acaaaatcga taacatttgt tctaagaatt agttacgtta aatacggaac
     5401 gcaagataga cttgaaaaac ggtaacaaaa gaagtagtat attaaggtgt gatgtgctac
     5461 ttgacattta ttctgctgag cgaactttct ggattgtgta gccttatatc tattcaggac
     5521 gtatcgagca gattagtaaa ggtgttcttg atgattttca tcttgaacac ctttgttttt
     5581 agcaattcgc aaaaaacgaa aagtaggaga attgataaat gaaaaaaaga tttttagcac
     5641 ttgccatcgt actaggcact ggcttattgt caggctgtac gaatgccggt gaaaaaacag
     5701 ccgttagtta taaaggcggt acaatcagcg aacaagaagt aatggatagc ttgaaaaaaa
     5761 tgcaaggagc agattccgct gtgcaacagt tgatcgttta tcaagtattt gaagataaat
     5821 atggagatga cgtatcaacc aaagaaatcg attctcaata tgatcaaaca aagaaacaat
     5881 tgggtgattc atttgattca caattgaaat ctgcaggtta cacagaacaa acattcaaag
     5941 acagtatcaa acaaagtcta gcctttcaag aaggtctaaa gaaacatatc aaattgaccg
     6001 atgaagattt aaaaacggct tgggaatctt tccatccaga agtggaagct caaattatcc
     6061 aagtagcaag tgaagatgat gcaaaagatg tgaagaaagc tgctgataat ggagacgatt
     6121 tctctaaatt agccaaagac aagtcaactg atactacgac aaaagaagat ggcggaaaag
     6181 tgaaatttga ttccactact acaacggttc cggcagaagt caaagaagca gcctttaagt
     6241 tgaaagatgg tcaagtttct gatgtgatca cttctaccaa tgcttcaact tatactacgg
     6301 aatattatgt ggttaaaatg gtgaaaaacc aaaacaaagg caacgacatg gataaataca
     6361 aaaaagaatt gaaagaaatt gcaacagaca caaaattaag tgacagtacc ttccagaata
     6421 aagtaattgg cgaagtattg aaagacgcta atgtaaaaat caaagacaaa gactttgaaa
     6481 atgttttgag tacattcact tcagattcta gtacagcaag tagtaaataa gcggtataaa
     6541 taaaaaatat gaactattta aatcaataaa actgttcatc taaatctttt tattagaact
     6601 aaa
//
LOCUS       DAFEOM010000109         6465 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.115, whole genome shotgun sequence.
ACCESSION   DAFEOM010000109
VERSION     DAFEOM010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.115"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(110..565)
                     /locus_tag="LP524_002646"
     CDS             complement(110..565)
                     /locus_tag="LP524_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235637.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(719..1927)
                     /locus_tag="LP524_002647"
     CDS             complement(719..1927)
                     /locus_tag="LP524_002647"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBL2235638.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2039..2371)
                     /locus_tag="LP524_002648"
     CDS             complement(2039..2371)
                     /locus_tag="LP524_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBL2235639.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2519..3394
                     /locus_tag="LP524_002649"
     CDS             2519..3394
                     /locus_tag="LP524_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL2235640.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3476..4057)
                     /locus_tag="LP524_002650"
     CDS             complement(3476..4057)
                     /locus_tag="LP524_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HBL2235641.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4063..5322)
                     /locus_tag="LP524_002651"
     CDS             complement(4063..5322)
                     /locus_tag="LP524_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBL2235642.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5324..6370)
                     /locus_tag="LP524_002652"
     CDS             complement(5324..6370)
                     /locus_tag="LP524_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235643.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
BASE COUNT         1801 a         1338 c         1102 g         2224 t
ORIGIN      
        1 aaaatcttgt cacagtccct tattctgaat aaacggtgtg gaagagacag cctatcagta
       61 gtaataagac acatctcttt cacagcctca tttttgcgta aaaatcgatt tattcgaaaa
      121 attccccgta tacatcttct acacgttgat ctggattgat cacgatatac aagttacctg
      181 ttttttcgct gatttttgtt gattgatatt gattgtctaa tccttctgtt cctttatcta
      241 gatatgggaa gtacacaaga tcaggtgtgc catttcggta aaggatttcc attcgctcga
      301 tatcttcgta tttcaatgcg cgattaaaca tgcccatttc cagtccgcct aggttgatat
      361 catgtgtcgc aactgcgtcc ccttcatgga agatttcgat tttaaatcca gcacacggat
      421 ggatttcaac aaattcactg ccattgattc gtccaaaact tgttgtgatt tgtttgatcc
      481 aaagatcgcc gatatgacgg cggttgatcg tccaggtttc tccatttcta aaataaaatt
      541 ttagcgctgt catttcttta ctcattaaaa agactccttt atgatgtaat ttattcttgt
      601 actacctcag tttagcacaa atttatgtaa gtggtcacat cttatgtttt ttcttctaat
      661 gacgtatctg cccactttaa gtagccaaca aagctgagca gatacagtct agtgtttttt
      721 aaatcaattt ttctttcgat aaaaaatcca aaacctgttt tttatcattt tttattcttt
      781 gttctacgac ggctttttca gctaattgca aagcttcact aacccactta ccaggttttt
      841 ttccagtttc ttctagcaag attttaccat ctactgctaa ttcgcttaat gacttgatag
      901 gtaaacgatc ataggattct cctacggcat ccatctgagg tgtttcgtca aaatagatca
      961 tcaattcttc tacttgcagt gcatgttttt tgcctaagaa gtacaattcg aatggttcca
     1021 aagctccagt ttctcgttgt cgtagtcctt ggacaagtgc ttgaacgtct ttaatcattt
     1081 ggttagaaca cttccacgat ttcaaaaagg aacgaatctc attttctttt agatccagtt
     1141 ggctgatcaa taatgcccat gcttggctct cttctttcaa tgggacggat ggtaattcag
     1201 caaaacgtaa aagtgcttcg ccttcttctt tcaaaccggg acagcaaatg tagcactctg
     1261 tttcaataaa tgcatggatt cccgcggccc gttcatttcc caagagcatc ttgatgaatt
     1321 ccacatttat acgttctaca gatattttac ctaatagctc gtggttttct ttgatggcgt
     1381 agaaggtttc ttcctctaat ttgaacccta gttgactgac aaaacgcaac ccgcgcatca
     1441 ttcgtaaggc atcttcatgg aaccgctcat gaggattgcc tacagctcgc aagatctgtt
     1501 cttctagatc ttgcagacca tcgaacaaat cgatgatttc tccatcttct ttcaaagcaa
     1561 acgcattgat cgtaaaatct cttcttttca aatcttctgc caacgaacga acgaattcga
     1621 catgatctgg tctacggtag tcctgataag ttgattccgt ccgaaatgtg gtgatctcat
     1681 attgttcatc attttctaat accaacacag ttccatgttc gatcccaata tctatcgttt
     1741 ttggaaaaag ctgtttgatt tcttctggaa atgcgcttgt tgcgatatcc acatcatgga
     1801 ttggctggtt cagcaagata tcacgaacac ttccacctac aaaataagct tcatatcctg
     1861 cattctctaa ttttttcaga actggaattg ccttttggta ttccagaggt aattgtttta
     1921 atttcatttt tccaaccttt cctgcgtgtt tcttgtcctg agataaagac tcagttgcat
     1981 ggtgactgtt tgacaagtcc gtttcagagt cttcaaggcc aggaccactt catcttgttt
     2041 actcgtcctt acgctcaaag cgattatggt ctctttgatt gaatttttcc atgttttttt
     2101 caaaaacttc agttaagtcg atattcaatg aattagccat gatcatcgta acgaataaaa
     2161 catcaccaag ttcttctgct accgagttaa ctggttcaga agcctttttg cttttttctc
     2221 cataagtgtg gttgacttct cttgccaatt cacctacttc ttcagttaat cgagccatct
     2281 gagctaatgg agagaaatac cccaccttga attgctggat atattcatcc acttcctgct
     2341 gcatactgta taaagaacgc tttttatcca tctttttccc tccaaaacta tcttaccaaa
     2401 actttaacgc aaatcctaga taagagcact tgagaaaatc gaatttccat gctacattta
     2461 ggaaggaatt tctaaatgga aattctgtga aaatacataa ttgaaagctg gaattcatat
     2521 ggtcaaacga aaaacagtaa aagaagttct ttttatcatt ataggaacaa gtatttatgc
     2581 atttggtctt gtttacttga acatcgctaa tcatttagca gaagggggag tctcagggat
     2641 caccttgatc ttaagagctt tatttggtat tgatccagct tatacgactt tattaatcaa
     2701 catcccattg attttactag gaggaaagat cctcggtaaa cgttccttgg cctatactgt
     2761 tctaggtaca ctttctttgt cattcttttt atggatctgg caaagggttc cactagaaat
     2821 caatttacag catgatctac tgatcgtttc tctacttgcg ggtttagttg caggtgtagg
     2881 aagcgggatc gtctaccgca tgggtggaac aacaggcggc agtgatatta tcgctcggat
     2941 tttagaacaa aattacggca tcactatggg gcgttcccta ttggcatttg atatcatcgt
     3001 cttacttgct tcattaacct atatcgactt gaaacgtatg atgtatacgt tgattgtttc
     3061 gtatgttttc agtcgtgtga ttgattcgat cttagatggc ggatattctg caaaaggtct
     3121 acttgtcgtc tccaacaaaa gcgaagagat tgcaccacta ctgatgaccg gtctgcaaag
     3181 aggtgtgact ttcctttctg gagaaggcgc attctctggt aattcaaaaa aaatcatcta
     3241 tatggtcgtt agctctagtg aattaactga agctaagagg atcgtccacg agattgacga
     3301 aaaagctttc ctttcaatca tcaatgtgca tgaagtggaa gggcaaggct ttacttattt
     3361 gaagccacag acaagattac tgaaaaaact ataagaaaaa agacaaagat aggctatgag
     3421 gaaagattcc acatccagct gacctttgtc ttttttagta ctatactatt tttaactaat
     3481 tgttatgttc acttacccgg ttacttttct tttgtgtctg caaaaggatc tttcggttaa
     3541 cttcatcaga taatttcaaa aaagcatccc tatctccatc ttctagtgct tgatcgattt
     3601 gagcataaag catttttagt tttgcctctt cttctttttc ttgaaaatat cgttcaatta
     3661 attcagtatc ttcttcactg actgaagaat cccaagaaga aaatggattg tcttctaaga
     3721 tagatagata ttctcttgtc tgccaagcat tttcgaatat acattccaca tataaaggct
     3781 ttttccaatt caaacggatt tcatgaaaaa tctggtctgt gtccgtaaat tcttttccct
     3841 gcaaaaacaa tttcatgggt tccccttcaa cagatagatc actgatctgc agtcctcgat
     3901 ccgcaaaatt cgccccttca acaaagtgga cattgcttaa aatcgcttca tgattggcaa
     3961 gataattcaa gatccaaaaa acttctcttc ggctaaaaga tacatgattg accatccacg
     4021 ttaaaaaatt cttcttttcc cgtacatcaa ttagcatttt tctcacctct ctgttaggtc
     4081 atctaataat gacagtgcct ccatatctcc tggttcaagt gttaaataat aagtcaataa
     4141 ttcttgcgct cttttcagct gcccttcttc tcttaagaaa aaggcatact ctttcaagaa
     4201 atctggttca tgtgatagtt catgataagc ctgttcatag tgtacagctg caacggtaaa
     4261 gtcttctaat tcattataag catgggctag gttccattct gcataaggat tagcagtttc
     4321 ttccatctga ttgatcactt gtatcacatc ttcatatctt tcatcatcca agtaaagatt
     4381 acttaaagta agtaatgttt catcttgttt gtctcctact tctaaagctt tcagtaaaag
     4441 ttcttctgct cgctgtctat catgcagacg gaaagcattt tccgaggcca ggtgataaag
     4501 ttcgacttgg taaggatttt ctttgattcc ttcttctaac actgtttgtg cttcttcaat
     4561 catttcttct tcttgaagag cttgtcctag atatagatat aacgattgat aatgcggatt
     4621 aacttctcga agttcttcca gatagttgat tgcctgctga ttttctttca attgaagatt
     4681 cgtaaaagct agctggaaca gtcgatcatc tgtcctttct tctttcagtg cttcttctaa
     4741 aggtacgata gctgcttcga attctccttg catacttaag gattgtccta aacgttcctg
     4801 catcgagatt cctgaaattt catttatccc gcttgccaac aatgtttcat aaaccactgc
     4861 tgcctctttg aaacgatcaa ctgaaaaata caattcagct agcgcaaact ggatcaatgg
     4921 ttcatcagga agcaacccag ctgcttcttt caattttgct tcactcactt cagggattcc
     4981 gataacttgg tacaaatcag ctatcgccaa taagctttgc ggatagtaat cactttgctt
     5041 atccactttt tccaggtatt caaaagctaa gtcgatctca ttatcttcga ttgcaatttc
     5101 tgctaacggg atgttcagtc cgtcattttc tgggtattgg ttgatcagtt gttcaaaaat
     5161 ctgttttgct tcgatcaaaa agccaatagc cagcaattct tcgcctagtt cagctaatat
     5221 atctgctggg tcttctttta atgctttttc taaaaagatt tgtgcttgtg acaagtcttc
     5281 atctgataaa gcttgcaaca ttttttcgct atttgtattc atttcaattc cctcttatct
     5341 cactgacgat ttgttgaaat accgtcattg ttctcaataa ttctttttcg tttggatgtt
     5401 ggtatccgtc tattatacct attttttcta aaacaagtat ttttttgctt ttttctgcca
     5461 aaaaaagaac tttttccaaa tactccatct ttgaaatctg ttccagccag tggattggat
     5521 agccaagcaa gtccagccac acaaaaaagt tttttagttg gaaatcaaaa ggatatgctt
     5581 ggagattcca cacgaaatga aacatcattc ctaaaaattt tttcatacta gcagaaagta
     5641 gatggccatt ttcggtcaga taaaaagctt gttcaaacag tttaccataa tttgacaggt
     5701 tttctgcctc taattgataa aaatagatca tttcttcgat catcccggca aacggccgag
     5761 tcgttaattg ctttttattt ggatagtttt tatacagatt ttgcaaaaaa cgatgatcac
     5821 aaatcattcc gcaacagata aaagtcaaaa agtcaatctg ctttccgtcc acctgccgct
     5881 cgctgattgt ttgatcataa acgatgcgtt ctggcagatt gatggtttgc agcactacac
     5941 gatttggtag ctgcaaaatc gtccgctgcg gtgttaatgc tcggcttaga gaacggatcg
     6001 atacagggat catccatagt gatgaatcaa gggttgtatg tttttgaaaa aaacctgcca
     6061 gatccatgat cccttcattt ccaaatccga tgataaacat ggattgattt tcgggatagc
     6121 gtttgacgaa atcaagcagg ttctttaatt ctgttaaatg attgcattga gtgttggcac
     6181 aaatatacca gtcattgttt gcttgatttg caaataacgg gttgatctta tctgcaaaca
     6241 aatcataata gcgctgattc gttaaaaata agattggctg atcaatggaa agttctctta
     6301 gctgactgac tagcgtttct ccgtaaaaca ctttcgtcaa cgcatcttct acttgataat
     6361 caagttccat acgcatccct cctcttctta tttttatttt accattagta agcaaattct
     6421 agaaaagata agagcaaagc tcacctcata acttggaaat aaata
//
LOCUS       DAFEOM010000110         6368 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.116, whole genome shotgun sequence.
ACCESSION   DAFEOM010000110
VERSION     DAFEOM010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.116"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            127..579
                     /locus_tag="LP524_002653"
     CDS             127..579
                     /locus_tag="LP524_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235644.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="LP524_002654"
     CDS             593..1282
                     /locus_tag="LP524_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235645.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="LP524_002655"
     CDS             1407..3758
                     /locus_tag="LP524_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HBL2235646.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKTIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="LP524_002656"
     CDS             3838..4233
                     /locus_tag="LP524_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235647.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="LP524_002657"
     CDS             4558..5175
                     /locus_tag="LP524_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL2235648.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="LP524_002658"
     CDS             5216..5515
                     /locus_tag="LP524_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HBL2235649.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="LP524_002659"
     CDS             5549..5716
                     /locus_tag="LP524_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL2235650.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..6324)
                     /locus_tag="LP524_002660"
     CDS             complement(5761..6324)
                     /locus_tag="LP524_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL2235651.1"
                     /translation="MIFKLRNRTEGLNIDKLFQEKASGKDTERPEFQKLLNYIREGDC
                     VIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSL
                     LSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQ
                     GIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2181 a          891 c         1262 g         2034 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 agactataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg
     6241 ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa gtccttcggt
     6301 tctgttgcga agtttgaaaa tcaaacgcgc cctctctgaa ctccaaatat cttaggctgt
     6361 tattccca
//
LOCUS       DAFEOM010000111         6286 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.117, whole genome shotgun sequence.
ACCESSION   DAFEOM010000111
VERSION     DAFEOM010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.117"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            <1..177
                     /locus_tag="LP524_002661"
     CDS             <1..177
                     /locus_tag="LP524_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235652.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="LP524_002662"
     CDS             190..690
                     /locus_tag="LP524_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBL2235653.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="LP524_002663"
     CDS             787..2634
                     /locus_tag="LP524_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBL2235654.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="LP524_002664"
     CDS             2637..3533
                     /locus_tag="LP524_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL2235655.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="LP524_002665"
     CDS             3583..3972
                     /locus_tag="LP524_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL2235656.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>6286
                     /locus_tag="LP524_002666"
     CDS             3959..>6286
                     /locus_tag="LP524_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL2235657.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2118 a          933 c         1348 g         1887 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagaggcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaac
//
LOCUS       DAFEOM010000112         6208 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.118, whole genome shotgun sequence.
ACCESSION   DAFEOM010000112
VERSION     DAFEOM010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.118"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            191..709
                     /locus_tag="LP524_002667"
     CDS             191..709
                     /locus_tag="LP524_002667"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBL2235658.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            837..1520
                     /locus_tag="LP524_002668"
     CDS             837..1520
                     /locus_tag="LP524_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBL2235659.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1829..1902
                     /locus_tag="LP524_002669"
     tRNA            1829..1902
                     /locus_tag="LP524_002669"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1863..1865,aa:Arg,seq:tct)
     gene            2061..2420
                     /locus_tag="LP524_002670"
     CDS             2061..2420
                     /locus_tag="LP524_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HBL2235660.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2543..4174
                     /locus_tag="LP524_002671"
     CDS             2543..4174
                     /locus_tag="LP524_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HBL2235661.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4236..4877)
                     /locus_tag="LP524_002672"
     CDS             complement(4236..4877)
                     /locus_tag="LP524_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBL2235662.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVPPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    5063..5139
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            5159..5467
                     /gene="rplU"
                     /locus_tag="LP524_002673"
     CDS             5159..5467
                     /gene="rplU"
                     /locus_tag="LP524_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBL2235663.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5494..5838
                     /locus_tag="LP524_002674"
     CDS             5494..5838
                     /locus_tag="LP524_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBL2235664.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5851..6144
                     /gene="rpmA"
                     /locus_tag="LP524_002675"
     CDS             5851..6144
                     /gene="rpmA"
                     /locus_tag="LP524_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBL2235665.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
BASE COUNT         1956 a         1023 c         1314 g         1915 t
ORIGIN      
        1 ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat aaaaaatgag
       61 ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca tttttacagc
      121 tataatttga aaaaaattta tcgttattat atgattaaga aggattgaat aaagaaagaa
      181 gttgaaaacg atacctgatt acaaaaaaat aacttttata agttctataa cgttgattat
      241 atttttaatt cttattcttc tcgtcgtctt ggttccccga atttttggtt tgactcctta
      301 tattattgag gacaaagaaa tgagtccata ttatgcacaa ggaaatctga tatatgtacg
      361 accgaaaaat acaaatgaga ttttagtcgg agatattatt acatattatg aaaattcagg
      421 aaaaaaaatc tcgacgcgtc gcgtaatagc cgttgaaaac aaccacgaag atttttatac
      481 aaaagctgat acaaagactt atattgagcc tggagtagta aaaaagagaa atattatcgg
      541 tagtcccatc tttcagattc catatattgg actagtcctt tcaaaaacgg catttgcatg
      601 gattcagggg ttattcttta cgattgcttt ttgcctaaca ggaatcacag catggaatac
      661 ctttgtagag ttgaaaaaga agcggagacg aacacagaaa tttttgtaag aatgttaaaa
      721 acataaatgt attccagaga tccaacgact cttctgaaca tgaaacacga gaaaacactc
      781 atgcagcttg tagatttagt cagttgttcg tataatgtgt gaagaggagg agtagtatgc
      841 gcaatgaaat gatgcatcgt cctgtagtca aagaagaact attggatttt atgcgtaccc
      901 agcaaaaaaa attaccgggt gagctaggca aactggaaga agaagctcat gtagcagaag
      961 taccagttat tccccatgaa acagttgtat ttttgaaatt tttacttgga cagcttcagc
     1021 ctgaacgaat cttggagatc ggagcagcta tcggtttttc atcaagtgtc atggccacga
     1081 caatcggaga aaacgctcat gttacgacaa ttgatcgttt cgatgtcatg atcgagaaag
     1141 ctaaaaaaaa ctatgaacgt ctaggactga cagataaagt gactttgctt gaaggtcaag
     1201 cagcagatat cttgccggag ttgtctggtc cgtatgactt tatttttatg gatagtgcaa
     1261 aatcaaaata tattgaattt ttacctgaat gcttacgttt gttaagaaaa ggtggcgttt
     1321 tgatggtaga tgacatcttc caaggaggaa cgatcttgct cccagatgaa gagattcccc
     1381 gtggcaaacg agctatccat cgtaaattaa atgaatttct tcgtgttgtg atggatcatc
     1441 ccgatttgac atcgacaatc ttgccattag gtgatggtgt gatcttaatg acaaaggaat
     1501 cagaaacaat cgagttgtga tacatgatta tggcagcctg tagataatcg attgaatcga
     1561 gggctcaatt cgaacttgat gtaaaagttt ctatatttgt aagtcaatta aaggatacag
     1621 gaaatctaaa taaacaggag tagcatgaag tcaatgtagc tcctgttttc aattgataac
     1681 cttagaacag ctgaaggaga atgtctcgtt ttaaaagatt gatttagtag ttgggccaaa
     1741 ataattcatt atttgctaat taaataacta tttcgtattg acagaagcaa tttaattatc
     1801 tataattatt gatgttccgc tgattcttgt catagtagct cagctggata gagcatccgc
     1861 cttctaagcg gacggtcggg ggttcgaatc cctcctgtga cgctaaaaaa ccgttagaaa
     1921 ctgaggtttc taacggtttt tctttgtttt ttgtgccaat attgtttgtt ttcaaaggca
     1981 aataaaataa aaaaacatcc ttatcgagta caattataag gagcaaaaaa ataaaactca
     2041 tcagaaaaga ggaagaagat atgacgatac tatctagaat tttagtgact ctagttgcaa
     2101 ttgaattttt ttatatcatg tatatcgaaa cggtgagaac tgattctgac acgacaagta
     2161 gggtttttaa aatgagtaaa gaagagttaa gcagaaaatc tgttcaaaca ttgtttaaga
     2221 accaaggagt gtacaatgga ctattaggag tgggactact ttatggagct tatctttctt
     2281 ccgcatctaa agaaatcaca agtatgctat taatatctat tttttttgtt gcattatatg
     2341 gcagtttgac tagcgatcgt tggattttac tcaaacaagg tgggctagct ctgttggcat
     2401 tgctttctat tttcttataa atatagaaaa aataagaatt tatctaacag gaaactttgt
     2461 gaaaaatatt actttatctc atgaaatatg atattctaat gaatggtatt ttagaaaatt
     2521 tcatgaaaaa ggagttagat aggtgttcaa aaattatgtt tcctcactaa aaaaagaatt
     2581 ttcaggttat gatctttcaa gatttcagaa agatcttttg gctgggatca cagtagctgc
     2641 tgtagcattg cctttagcct tggcttttgg ggtatcaagc ggagctgatg cggccgcagg
     2701 tttgattacg gctatcattg ccggtatcgt tattggcggt ttatcaggcg gtttttatca
     2761 aatatcaggt ccgacaggtg cgatggcagc tatcttgatg tctattgcgg caacaaaggg
     2821 aatgcaaggt gtattgcttg ctactttttt agcgggtcta tttttacttg cagcaggact
     2881 tttccgatta ggaacactga cttcttttat cccagcaccc gtaattacgg ggtttacttc
     2941 aggtattgct atcattattg cattaggtca gatcgataat ttatttggtg ttcactcgga
     3001 aggtacaaac gttattgaaa aactaagtag ttatcaaacg ataggatttc cgataaacac
     3061 agcctctttg atcatgggag ggctagtcat tttagggatg tttatctatc caaaaaaatg
     3121 ggcgaaacga atgcctagtt cgctgctggc tatcattttg gcaacagcag ttatgctatt
     3181 gttccattta ccagtagcaa ctgtgggtaa gattccacaa acattgatca gtagtaatcg
     3241 tttgaatatg ggagatttta gcttttctgc cttgcaacaa gtggcagttc cagctattag
     3301 cattgcttta ttaggaatga ttgaaagctt gctatgtggt gcttcggctg gccgaatggc
     3361 aaataagccc ttagatagca atcaagaatt aatggcgcaa ggaattggaa atctgttgct
     3421 tcctttcttt ggcgggattc ctgctactgc ggccatcgcc cgtacaagcg tggccatcaa
     3481 atctggtgca caaactagat tagctggtat gatacatgca gttgtcttac tgctctccat
     3541 gcttgttttt gcgccagtga tgtcacagat tccaatgcca gcacttgcag gtgttttgat
     3601 tgtgacggct tggcgtatga atgaatggga aacgatcaag gagctattta ctaataaata
     3661 ttggtcagca gttctattgt ttatccttac gatgatttgt acagttatct ttgatttaag
     3721 tattgctatc gtgatcgggg tgatcggcgg ctgtgtattt tttgttgtca aaagcgctgc
     3781 tatcacgatt tcattagaaa cgatcgattg gcaaagaatg gggctgccag aaacggaaaa
     3841 gctgaataac tggacagtag tgtatattag tggtccactg ttttttatgt cttcagaaaa
     3901 actcaaaaat aaattggaag aactaaatca tcacgatggt gtcattttct ccatgcgagg
     3961 cgtaccaagt attgatttga cggcacgtgc agtttttgat gaatttcaag aaaaagcaga
     4021 gcaaagagga caaacgatta tctatacttc cttgcagcca gaagtagaaa aacaactttc
     4081 tcacttgtgg gaacatacat tcacgcatca acatcaaaca gttgctcatg cgttgacagc
     4141 tttacatgcg aaatatactg caattagcca ttaaaacaat gaaaaagaac agacaagact
     4201 gtaaactgaa gattttatgg tcttgtctgt tctttttaat attgctgtaa aagtgaagct
     4261 agtacttgcc agtcttgatg gataacaggt acaagggagc gattgtagaa aatagctgct
     4321 ggatgaaaaa gtggaagaat tcgatagttt ttttctgacc attcgtaggc atccttttgt
     4381 tctgttaatt gtaagacagg ccctgtatag agttgaccat gatttttggt aaccgtatat
     4441 tctttgccaa gtaaacgttg aagaccaatg tttccaagtg tcgcaattaa ttttggcggg
     4501 accttcatca gttcgtaatc caatattggt gcatgcgcaa atacttcgga acgagtaggt
     4561 gtccgatttg gataaacagt ttctgactgt tgtgtccgtt tattgatccg atgagtcacg
     4621 cgatacgggc ggctccttac agcactggtg atatacacct cttctcgtgt taagccagtt
     4681 gaagaaagtg cctgcatcaa ttcttttcca gcttgaccgc taaaaggaac atgcgtatca
     4741 atctctgttt tccctggggc ttctcccacg agcatcagtt taggatgaag aggtccttgc
     4801 ccagcgataa atccttctac aggacggccg ttcattctta aagaagcttc ttgtatcagt
     4861 tcttttgggt attccattta tgctcctcct ttttctttta ttataagaat aaaagaaaca
     4921 caatgcgact attttatttg gtagaactgt gaggatagaa aagcagaagt attgcttgaa
     4981 tttttgtttt actttttgat tttaaattta gttgactaac ggttgctgtt ctggtaagct
     5041 agtactgttg taattgtgga ctccacagct acaaccgctc agaacaagta ataagcacag
     5101 catatgctgt cacttcggat ggcgagtcta agtatatcag aaggaggtgc tttatagcat
     5161 gtacgcaatt attaaaactg gtggtaaaca agtaaaagtt gaagtgggac aagcaatcta
     5221 tgttgaaaaa cttgacgttg aagcaggaga aaaagttgtt tttgacgaag ttatcttagt
     5281 aggcggagaa tctacaaaag taggcgctcc aactgttgca ggtgcaactg ttgaaggtac
     5341 tgttgaaaaa cacggcaaac aaaagaaagt cgttactttc aaatacaaac ctaaaaaaca
     5401 ttcacaccgt aaacaaggtc atcgtcaacc atatacaaaa gtggttatcg acgcgatcaa
     5461 cgcttaatag cgtttgagaa agaaggccta tatatgatca aaggaacatt taagcgaaat
     5521 gacgcaggtc agatcgtttc attcactttg accgggcatg ctgatgctgg tccttatgga
     5581 agtgatatcg tttgtgcggg tgtttctgcg ttagcgatca gcactgtcaa cgggattgcc
     5641 tcattagccg gttttgagcc gattgttgaa atgaatgaag aagaaggcgg ttatctttat
     5701 accgaagtca cttcaggaat gacacaagaa caaaacaata ttgctgagat tctattagaa
     5761 aatcttttat taggattaca atcgatagaa gctgaaaact cagaatatat ccaaattaaa
     5821 acaattaatg ataaatagga ggtgcagacc atgttgttat ctatgaatct acaattattt
     5881 gcccacaaaa aaggtggcgg ttctacatct aacggccgtg actcagaatc aaaacgtcta
     5941 ggtgctaaac gtgctgatgg acaaacagtt acaggtggat caattcttta ccgtcaacgc
     6001 ggtacaaaaa tctatccagg tgtaaacgtg ggtatcggtg gagacgacac tttatttgca
     6061 aaagttgatg gcgtagtacg tttcgaacgt aaaggccgta acaaaaaaca agtgtctgtt
     6121 tatccagtag ctcaagaagc ttaattgaat cttatgatta aagaggttgt gacaagactt
     6181 ttgtcacagc cttttttgtt tataaaaa
//
LOCUS       DAFEOM010000113         6173 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.119, whole genome shotgun sequence.
ACCESSION   DAFEOM010000113
VERSION     DAFEOM010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.119"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            725..811
                     /locus_tag="LP524_002676"
     CDS             725..811
                     /locus_tag="LP524_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBL2235666.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="LP524_002677"
     CDS             complement(932..1621)
                     /locus_tag="LP524_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235667.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="LP524_002678"
     CDS             complement(1628..1906)
                     /locus_tag="LP524_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235668.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="LP524_002679"
     CDS             complement(1866..2780)
                     /locus_tag="LP524_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBL2235669.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="LP524_002680"
     CDS             complement(2762..3142)
                     /locus_tag="LP524_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBL2235670.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="LP524_002681"
     CDS             complement(3592..3879)
                     /locus_tag="LP524_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBL2235671.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="LP524_002682"
     CDS             complement(3899..4123)
                     /locus_tag="LP524_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HBL2235672.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="LP524_002683"
     CDS             complement(4578..4748)
                     /locus_tag="LP524_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235673.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="LP524_002684"
     CDS             complement(4763..5296)
                     /locus_tag="LP524_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBL2235674.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="LP524_002685"
     CDS             complement(5317..6159)
                     /locus_tag="LP524_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBL2235675.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1634 a         1152 c         1041 g         2346 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtacatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAFEOM010000114         5881 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.120, whole genome shotgun sequence.
ACCESSION   DAFEOM010000114
VERSION     DAFEOM010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.120"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(1..191)
                     /locus_tag="LP524_002686"
                     /pseudo
     CDS             complement(1..191)
                     /locus_tag="LP524_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            212..1414
                     /locus_tag="LP524_002687"
     CDS             212..1414
                     /locus_tag="LP524_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HBL2235676.1"
                     /translation="MCTKLLQNTAHKNPYKTVDEATQLDLVIVVNQLLTGYDSKWVNT
                     LYLDKSLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEAIELYSGKQAK
                     GLFVEKLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVNLYNQLIHCYE
                     SAMIQGFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIEREPRDGNDSIAYDIDY
                     QLPEGSRSRINYEYLNTNFKKYMNIKEERDSNLIQSLLNDLHRTFAQLPVELQPYAEM
                     ILNDVNNGELVIKEGWEFTDYLNEYQLSEEDDFIQDLVDSTNINEALLREMLDLRLTE
                     VNINEYSRFDKLKSSVNVGHFSGYIEKNLGKMVIPIKVNMLIDRLLRAFLLEDVFDVT
                     EYIKNYFN"
     gene            2147..3115
                     /locus_tag="LP524_002688"
     CDS             2147..3115
                     /locus_tag="LP524_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL2235677.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            3126..3941
                     /locus_tag="LP524_002689"
     CDS             3126..3941
                     /locus_tag="LP524_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HBL2235678.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKKIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            4009..4719
                     /locus_tag="LP524_002690"
     CDS             4009..4719
                     /locus_tag="LP524_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL2235679.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            4755..>5881
                     /locus_tag="LP524_002691"
     CDS             4755..>5881
                     /locus_tag="LP524_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBL2235680.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINYFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIK"
BASE COUNT         2020 a          883 c         1187 g         1791 t
ORIGIN      
        1 gttttaagca actgacaata gctggcattc atgtccatca ctaggtactt gacctttaaa
       61 cgggcctttc taggaaagcg caggaagtat tgagttagtt tgtaaagtct gcgatcttca
      121 agggtcgtaa atagctgatt ggttgtccca tcaacacaaa taaaactcat ggaaccagaa
      181 gtggcaacat tgtgtacttt tctttgcaat cttgtgtaca aaactattgc aaaacacagc
      241 gcataagaat ccttacaaaa cagtagatga agcaacccaa ttagatcttg ttatagttgt
      301 taatcagctc ttaacgggtt acgattcaaa atgggtcaac accctatact tagataagtc
      361 acttgaatat cagaacataa ttcaggcttt ttcacgcaca aatcgtctta atggtcctga
      421 aaaaccgttc ggaaccattc gttattatcg taaagcacat acgatggaac agaacattga
      481 agaggctatt gagttatact caggcaaaca agcaaaaggg ttatttgttg aaaaattata
      541 taataactta gaaaagttga acgatatatt cgaagaaatt aagtccgtat ttaatatttc
      601 tggcatagaa aacttcagtc atttaccgga ggttccaggg gaaattaagc aatttgtaaa
      661 tctttataat caactaatcc attgttatga atcagcgatg atacagggat tcacatgggc
      721 aaaatcgact tatgaaatcc aggattatga acctataaca gtagtaatca cagaatatga
      781 atttaatatc ttggcacaac gttacagcga gatagagaga gaacctagag acggaaatga
      841 tagtatagcg tatgacattg attaccaatt acctgaaggt agtcgtagtc gtattaatta
      901 tgagtattta aacacaaact tcaaaaaata catgaatata aaagaagaac gtgattcgaa
      961 tttaattcaa tcattgttaa acgacttaca tcgcacattt gcgcaattac cagtggagtt
     1021 acagccatat gctgagatga ttctaaatga tgtaaataat ggggaattag ttatcaaaga
     1081 aggatgggaa tttactgatt acctcaatga atatcagctt agtgaagagg acgattttat
     1141 ccaagattta gtggattcaa ccaatattaa tgaggcatta ttacgtgaga tgttggattt
     1201 acgcttaacc gaggtaaaca ttaatgaata tagtcgattt gataaattaa aatcaagtgt
     1261 taacgtaggt catttcagtg gatatatcga gaaaaattta ggtaaaatgg taataccaat
     1321 caaggtaaat atgctaattg acagattatt gcgtgcattt ttattagaag atgtattcga
     1381 tgttacagag tacataaaga attactttaa ctagagtaca cctacatctt atagtacata
     1441 aaaaaattca tcttttttag tttaataatt caccaaaaag gtgagtaatg ccatttttga
     1501 aaattgagct tgttttatgt ctacatctgt atccgtatat aatccaaagg attataaact
     1561 cagaagagtc tttatttttt ctgaatataa ctactgtatc aaaaacaatc gattatgaga
     1621 caaaacaaaa tgacctcttt tatttaagaa aggaggtcat tttttgtatc ataaattgta
     1681 ttaaaatcaa tgtatcactt tttaatcaat tttaagacca taaattgcgt actaaaagaa
     1741 attctgcaat tcaaaataaa gcattttaag ccattttcaa atttaagtgc acccatctac
     1801 ccgtcaaatc actttatttt ccactccagt agtttcctaa ttcaactgtg tgcaaaacga
     1861 tttcttctaa acaaatcaag acaaacttat aaaccttcat cccagcacca taattattta
     1921 aatcataggc atttttcgaa attagtatat gtactcaagt ttcactgcag attatttata
     1981 ttttttagag aaataaattt ttcctattga caaacacaaa gaaagcggat acaatacaat
     2041 tatgtcacat gacgttatat gacgttatgt aatatcttta catttaatca gttactttta
     2101 ttttggataa aattaacatt tttaaaattt taaggaggaa atgttgatgg atgtagcaaa
     2161 aacggttaac gcaattgtag aaaaaaatga tggagagatt tctggaattt atttggtggc
     2221 ttgtggcggt tcgctggtag atatgtatgt ctcagattat tttcttaaag ccgaagcatc
     2281 gaagtgtatt acaggcttat atacagcgaa cgaatttaac catgtaaccc caaagcaatt
     2341 agatgataaa tctgttgtca ttctatgttc tcatggagga aatacagcag aaacggtaaa
     2401 agcaggagaa gtcgctcaag ctagaggagc tgtgactgtt ggtctgactc ataataaaac
     2461 tgcggattta ttaaaggttt ctgatcatag tttcctttac gaatggggag atgatgcaaa
     2521 aatagtcaat aatcctatgg ccatcattct tgatttaact gtatctttgg tcaatgttac
     2581 cgaaacattt gaacattacg gtaaattcag agaaggcatg aaggttatcg ataccgtaat
     2641 tgaaaatggc attaagcagg tgcaggatcg tattaaagtg tttgctgata agtacaaaga
     2701 tgaaacaatg ttttatatct taggtagtgg accatcatat ggacatgctt atggtttttc
     2761 aatttgctct ttgatggaaa tgcaatggct tgattcaact tctgttcatt ctggagaatt
     2821 cttccatgga ccttttgaag taacagacaa agatacgaat tttattctct tgatgagcat
     2881 tggacgcact cgtgctttag atgaacgggc aaaacgattc cttgataaat atgcagaaag
     2941 agtagaaatc gttgatgcaa aggaactagg attggatatg atatcagatg aagtttctga
     3001 attcttcaat cccattttat tctacagtat tctttcagaa tatcgttcag ctttagcaga
     3061 tgcacgcaat catgatttag atgttcgtcg ctatatgggg aaagtggagt actaggagga
     3121 ttaaaatgaa ggtattaggc ttaggtgata acgtcgtcga taaatatgaa aatctgggaa
     3181 ttatgtatcc tggaggaaat tcattgaatt ttgcagtttt tgcaaaaaaa ctgggacatg
     3241 aaagtagctt tatgggagtt tttggctcag atgaggaggc agctcatgtt ttatcagcaa
     3301 taaaaaaaat aggactggac aacagtcact ctcgctttga aaaaggcgag aatggctgtg
     3361 ccagagtgaa gattgatgag ggcgaccgga ttttccttgg cagcaattcg ggaggtgtta
     3421 ctcgtgaata cccgattcag cttactgaag aagacagaga atatttaaac cagtttgaat
     3481 tgattcatat gggattatat agccatgtca atcatctatt agaagagtta caaaatgttt
     3541 ctggaaagat ttcttatgat ttttctgacg actttacgga agaagaggtt cagcgggcaa
     3601 ttgataaagt tgattttggc ttcttttcta tagaagactt ttccgatgaa gaagtaaagc
     3661 agtttttgat gaagcatttt tgtgagcgaa atgatgtatt gattgcaacc cgaggagaaa
     3721 aaacggctat tggttatgat ggagcaaagt tttatgaagt gaaccccgtg ctagaagaac
     3781 cagttgatac aatggcagcg ggagattctt tcttaacagc tttcctactt tattttctcg
     3841 aaggggacga tatagtcacc gcgatgaaaa aggggaatga atttgctggg aagtcttgtc
     3901 tggttgacgg ttcgttcggc tttggtattc attacgattg agcttgtttt aaattcctgc
     3961 atttgataac ctaaagaaag aaatcccggt aaagtggagg ttaaaaaaat gctggataca
     4021 aatgcacaat taccattgta cttacaatta aaagaaatat taaaaaataa aatcaataca
     4081 ggggagtatc ccgagaatac gaaaattccc gctgaatcag agctgataga aagttatgag
     4141 gtcagccgaa tcacagtgag aaaagcagta gaagaactgg tagaagaagg gtacctactt
     4201 aaaaaacaag ggaaaggaac ttttgtcaac cggcataaag tttttcgtaa aattgaatat
     4261 gttatgggtt tcagtgagag ttgtaaatct aatggtttta caccgactag cattgttttg
     4321 gaacgcaaga tcattccagc aactgaaggg atggcaaaaa aactcgatat cgctcaaggt
     4381 gatgatgttt tatatatcca gagaaagcga atggcggata acatccccat tatgttagaa
     4441 aacaattatt tttcttatga aagatatagt ttcttaatgg aggaagagct gactgattct
     4501 ttgtatgaag tacttaaagt ccaaggtacc ttagccatca atcctgggga aactgcactt
     4561 gaaattgtca cagctgatga ccaaattgcc aaaataatgg aagtagctgt tgggacgccc
     4621 tttttcttta tggatacttt aatcaacgat caaaacaaca taccaattca tgtaggtaat
     4681 caatattacc tgggagaagt atatcgtttt tacttatgat ttcctgtttt gatttcaagg
     4741 agtagatttt atttatgaag caaatattgc ggtcaaaaat gatttttgat ggagagcagc
     4801 cacctttcaa aggttatatt gtaatcaatg aagggatcat tgaaaaggtg gaaaacaact
     4861 ggaattacag tgatctatta aatgaaaaca cggaattaat tgcttatgat gatcaattcg
     4921 ttatgccagc aattcatgat aatcatgtat ttttcagtgg ttatttggct atgaatgctg
     4981 gcatagattt aagcgataca tcttcagcta acgaggctgt agagcgtatt aaagctgtaa
     5041 tgaaccataa aaaagcagat gaaccaattt atgctcatgg ctgggatgct gccctgtgga
     5101 aaactgaccc atcagaagag ctattgaatg aaataaacta ctttggtcca atcacagcga
     5161 tcgacaaaaa tcgcagccat tgttggatga atgctattgc taagcagact tatggtttta
     5221 cagaaaaaga aaccagtgct gaaagtcgag tattattaat taaggaaatg ctagcaaaca
     5281 aaaagctttt agcagaggtt tacgctgaat ttgaagcatt actgctttca aaaggtgtta
     5341 tatcaatgaa agaaatcgtt tttgatgacg cagaatttct ttctaatata cctcagagga
     5401 aaatacttag taacctatat gtgcaggcag tccaagaacc tctacagcat gaacttctat
     5461 atgagtataa ggaaaaagtt ttccctgaaa aggtccgctt tggtggagtg aagatcatgg
     5521 tagatggtgt tgttgcggat agaagtggag atatttacgg tgtttatcag tcaggagtag
     5581 cttcacccga gattgactat gaggcaatta aagcagaagt tgctgcattc aatgaagtag
     5641 agattccttg ctgtttgaca acggaaggag ataaagcggg ttataaagct gcgtcaattc
     5701 ttgtcgaatt tggaaagcgg ttacctgaag gcgtttttaa ttcgattagt gatttggaaa
     5761 tggttactca aaagacagca gaaaaaatga acgaaggaca agtagtagca gaggtatatc
     5821 cacaaattct cggcttgaat caaacgttag aagaggctta catgtcaact gttataaaaa
     5881 a
//
LOCUS       DAFEOM010000115         5877 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.121, whole genome shotgun sequence.
ACCESSION   DAFEOM010000115
VERSION     DAFEOM010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.121"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            641..1375
                     /locus_tag="LP524_002692"
     CDS             641..1375
                     /locus_tag="LP524_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL2235681.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1391..2560
                     /locus_tag="LP524_002693"
     CDS             1391..2560
                     /locus_tag="LP524_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL2235682.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2576..3571
                     /gene="lacD"
                     /locus_tag="LP524_002694"
     CDS             2576..3571
                     /gene="lacD"
                     /locus_tag="LP524_002694"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HBL2235683.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYAEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3650..4576
                     /locus_tag="LP524_002695"
     CDS             3650..4576
                     /locus_tag="LP524_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HBL2235684.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4595..5845
                     /locus_tag="LP524_002696"
     CDS             4595..5845
                     /locus_tag="LP524_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL2235685.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1967 a          956 c         1203 g         1751 t
ORIGIN      
        1 gtttgaaaaa tgctatttcg aggtagaagg atattatata agcacggttg actaaacgaa
       61 gtgataataa aaaaagttat tcaagaataa gaaaaattat gatacgaatt cagataaact
      121 ggagaataca gagaccttta aaagggataa gaaacaacat aaattcctct taagagagaa
      181 cgaaaggaca aagccagcaa cttgatacac gagctagtat tagaggaatt tattctcata
      241 gactcccttt tgaatacgtt tcaactttaa ttttcatgtt cataaatgat ttgaaaccaa
      301 agcaaatgcg ttgcactttt cctccgtctt ccgaaaatcg ttcaattcac tggtaagtag
      361 tcatttgata gtttgaggca aattgttgat tgatgaaggg taggagctaa aaaaagatac
      421 cagttgaaga gagaaaaaga aaaaaataga gggtgttctt gaatgaaaaa agttggaaaa
      481 ttatttttca tttcctttta ttcctattaa agtcagtgat gtcaatcata ttaacaagac
      541 taaaaatgaa aataaaaaaa agattgttct tagtattgac tcataaaaaa taccactact
      601 atacttacat tgtggttata gccagttagg agagacatcg atgcaaaata cctttcaatc
      661 aaaatcactt tattatcagc tggtagaaat attgaaacaa cgaatcgaaa acagtatggt
      721 cgctcatgac aagctgccat cagaaagaga gttgaccagt ttttatggcg ttagtcgcac
      781 gacggtgcga ctagcattgc aagaactgga aaaaatgggc tacatttatc gacaacatgg
      841 aaaaggaacc tttgtttcgg aattaaggac aaatgcgact gatctagctg gcgcatatag
      901 ttttaccgaa caaatgcggg caatgggcaa acatcctgaa accaagatat tggccttcaa
      961 gaagattgaa gcaactaaat atttagcgag tcatctgaat gttcaactag gtgagccttt
     1021 atttcaatta gaccgattgc gcttagctga tgatttgcca atgatgatcg agaaatccta
     1081 tttgccagtg aagctattta tgtccctttc tcaagaaatt ctatttctga aacctttata
     1141 taatttgttt tcagagaatt tcaatcaaat cgtacggttg gctgacgagg aattttatgc
     1201 cagcattgtc cagaaagaag atagtgagtt gctaaatatt ccagaaggat caccagtgct
     1261 ccatttagtt cgctcaacct ataatctcga gaacgaagtg attgaattta cattaagtgt
     1321 agcgcgggct gataaatttc attataagat caggcatctg cgttccacta actgaattgg
     1381 aggaaagaaa atgtttatgt tctcaaaaga agaactacga aagctaggag cagaaaatac
     1441 tacccgagaa attcgacaac aaccggaact gtggcatgag acgtttgacc gttatcaaga
     1501 aacagttgca gatttagatc gtttgtttac ggatgcgaaa caacatgctg gagagcgacc
     1561 aatccgagtc atttttacag gggccggaac ctcgcaatat gtgggagata cactaattcc
     1621 gtttcttact agtcaaggaa atacgaacca gtttattttt caaagtattg gttcgaccga
     1681 tatcgttgct acgccaaaag aatacctgat tcctgatgag acaacgatct tggtctcatt
     1741 tgcccgtagt ggaaatagtc cagaaagtct agccgcagtt aagatggcca atcagctggt
     1801 caacaagatc tatcatatcg tgatcacctg tgcaccagac ggattattgg ctacacaaat
     1861 gcctacaaaa aaagaaaatc tagttttact aatgccaaac aaagccaatg acgctggttt
     1921 tgctatgaca agcagtttca gctgtatgac gctgactgcg ctacttattt ttgatcaagc
     1981 aagcaatcag caaaaagaaa gatatgttca tcaattaagc cgactaggag aagaagtcat
     2041 ccaacgagaa tcagagatcc aagggtatct ggcatcagac tttcatcgca tcatttactt
     2101 aggttcggga agcttaggcg gattgactag agaagcgcaa ttaaaagttc tcgagttaac
     2161 tgctggaaaa atcgcaaccg tatttgatac atctttagga ttcagacatg gtcccaaatc
     2221 ttttgtcaat gagcaaacat tggttttcct gtttgtcagc aatgactcat atacgcagaa
     2281 atacgatgta gacatgctgg aagaaatcaa aaatgacggt attgcccaac gcgttgttgg
     2341 aattggtcaa aagggttcat tgtttagtgg tgattccttt gaattagttg cagattcgtt
     2401 actgccggat ggctatcttg ctttagcaga tatcatggta gcccaaacga tcgcattact
     2461 ttgttcggtg aaggttggta atacaccaga tacaccatcg ccaagtggaa cagtcaatcg
     2521 tgtggtcaaa ggcgtaacga tttatccata tgaaaaatag gaaagagtgt gaagcatgtt
     2581 agaatggtcg gaaaataaaa gacaagcaat ggaacgtcta tcgacgccag aaggcattat
     2641 taccgctttg gcgatcgatc agcgaggcgc gctgaagaaa atgatgtcgg ctttagggat
     2701 ggaagcgaca aaggaaaaaa ttgagcagtt taaaacacta atttcccaag aattgactcc
     2761 ttatgcctca gcaattttat tggatcctga atacggactg cctgctgcac agaagcgtgc
     2821 agcagcgagc ggtctgctgt tggcatatga aaaaactggt tatgatcaaa ccgtacccgg
     2881 ccgcttgccg gatctgctgt cggactgttc agttctgcga ttgaaagagt taggtgcgga
     2941 tgcggtgaaa tttttactgt attacgatta tgcagaagca gaggaaatca atcggcaaaa
     3001 gcaagttttt gttgaacgaa ttggaagcga atgcatggct gaaggactgc cattttattt
     3061 agagttattg tcatatgatg ctaaaaatgt agatacgaaa tcgctttcct atgccaaagc
     3121 aaaaccgcgc aaagtgatcg aaatgatgaa agaattctcg aaaacccagt ataaagtaga
     3181 tgtcttaaag gttgaagtac cagtaaacat gtcatttgtt gaaggaaatg caacagggga
     3241 gatcgcctat acacgtcaag aagctgccgc ttattttaaa gaacaaagcg atgcaacaga
     3301 tttgccgtat atcttcttaa gtgctggggt atcagcggca ttattcatgg agacattgcg
     3361 ttttgccaaa catgctgggg cacgattcaa cggtgtactc tgtggcagag caacttggaa
     3421 agatgcagtc gctcctttcg ttgaaaaagg agaagcggca actttagaat ggctgacaga
     3481 acaagggaca caaaacattc gtcaattgaa tgaagtgata agagaaactg ccatcccatg
     3541 gtatgagaaa gtaagtgaaa gcacttgtta atgtttctaa tatattagta gtgataagag
     3601 atatgtgtga ttataattta tcattattga aactgggagg atattttata tgaataaaag
     3661 attgttagat tattcagctt cagatttttt gaaagctaag ccgatggaat taaagcaggc
     3721 aattttggct tctgaaggta gaacaattat ggcagaaaat gtgccaagta gctgtcctta
     3781 catgggaaaa tcagtgacaa atgcagaatt ggagagagct gctggagctg atttaatttt
     3841 gtttaatgca ctagatcttt ttaatcctca aatttcaggt gtacctttaa ctctaaaaga
     3901 gaatccaata atctggatta aaaaagcttt aggaagagct attggtgtaa atttagaacc
     3961 tgtagacaaa gaagttttga tgagtgaaaa tcgaactgaa atttcaagtg gtagaatcgt
     4021 ttctcctgag actttaaaaa aggcaaatga attaggcttt aattttattt gtttaactgg
     4081 aaatccagga acaggagtat ctaatagaac tattgagaaa gcaataacat tatccaaatc
     4141 aatttttaat ggattaatta tcgctggaaa aatgcatggt gctggtgttg atgaaccaat
     4201 tatgaactta gatatagcaa aaagatttat taaaacaggt gtagatattt tattagttcc
     4261 tgctccatac actgttccgt atttcatgga aagtgatttg cgaggaatcg ttgattatat
     4321 aaaagatttt aatagaaata aatctattga ggaaaaagtt ctagtactaa cggctaatgg
     4381 aacaagccaa gactcttctg accaagaaac tattaaaaaa attgcattag catcaaaagc
     4441 atgtggagct gatattcaac acatagggga ttctttaaat ggtatttgtc ttccagaaaa
     4501 tatttttgct ttgggtcaag caattcgggg atatcgtcat caaattatta tgttaggaaa
     4561 atctaattat cgttaattaa ggaggaaatg aaagatgatc gacaaagttc aaaaaatagc
     4621 tgcaaaaatt agtaatcaaa gacatctact agctttaaga gacagcatgg ctgcagttat
     4681 gccattaatc attattggtt ccttttttat gttattagca aatttcccga tagaaggatt
     4741 tacaaaatgg ttatctgaaa ttggagtaca atcctttttg aacaaagcat cagattcaac
     4801 atttggtatt attggattag ctgttaccta ctctttggga cacaatctag caaagactta
     4861 tggtgtagac tcattatcag caggattatt atctttgagt ggatatgttt tacttacacc
     4921 tttattggat gcagatggtt ctataggatt tccaacaaaa tactttggta cctcgggtct
     4981 ttttgttggt attattgtag cacttgtatc tacagaaatt ttccggtggt ttgtacttaa
     5041 aaatattaca attaaaatgc cggcaagcgt tccaccaaat gtaagccgag ctttcgttgc
     5101 tattattcct ggcttttttg tcgttctact atggtttatt gttgttgtaa tttgttataa
     5161 acttggaatt gaaaatgttc atgctcttat tgcagataca ctcgcaaaac cactatcttt
     5221 actgacgaaa actttaccag gaattatatt ggttatactt attcaatgtt tcttttggat
     5281 gtttggtatt catggtgcac aggtaacggg gccaataatt gaaccgttat tattgcaaaa
     5341 ctctgatgta aatagaattg cttatcaagc tggaaaagaa cttcctaaca ttataactta
     5401 tgaattctta tataactttg ttttttctgg tggtgcaggt tgtttatttg cacttgccat
     5461 tttaacattt ttcttttcaa agagtcagga aaataaaact ttaggtaagt tatcaatcgc
     5521 tccagtaagt tttcaagtag cagagcccct tctatttggg tttccaacta tattaaatat
     5581 aaaaatgtta attccatttg taactgcacc agtagtaaca acattaatta cttatttttc
     5641 aatgagtatg ggattagttg caaaacctgt aggagcaaca atcccatgga caactccacc
     5701 gataattgcc ggatttttag cttctggtgg acgaataagt ggtgcagtga ttcaagtaat
     5761 aacaattgca ataaatgttt tgatatacta tccattcttt aaattagatg ataatgccaa
     5821 gctaaaaagc gaaaaaaatg attagaacta cctatatgtt tctaactgta gttaagt
//
LOCUS       DAFEOM010000116         5501 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.123, whole genome shotgun sequence.
ACCESSION   DAFEOM010000116
VERSION     DAFEOM010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.123"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            153..1142
                     /locus_tag="LP524_002697"
     CDS             153..1142
                     /locus_tag="LP524_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL2235686.1"
                     /translation="MRDKEMRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAG
                     QYLFDKELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGA
                     MTICLSNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWET
                     CLNEFDKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFME
                     MQWINSNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVL
                     DQADFDLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1124..1585
                     /locus_tag="LP524_002698"
     CDS             1124..1585
                     /locus_tag="LP524_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBL2235687.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1635..2099
                     /locus_tag="LP524_002699"
     CDS             1635..2099
                     /locus_tag="LP524_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL2235688.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2113..2862
                     /locus_tag="LP524_002700"
     CDS             2113..2862
                     /locus_tag="LP524_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL2235689.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2862..3692
                     /locus_tag="LP524_002701"
     CDS             2862..3692
                     /locus_tag="LP524_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL2235690.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3692..4339
                     /locus_tag="LP524_002702"
     CDS             3692..4339
                     /locus_tag="LP524_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL2235691.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4437..5213)
                     /locus_tag="LP524_002703"
     CDS             complement(4437..5213)
                     /locus_tag="LP524_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL2235692.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5295..5438
                     /locus_tag="LP524_002704"
                     /pseudo
     CDS             5295..5438
                     /locus_tag="LP524_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
BASE COUNT         1882 a          747 c          979 g         1893 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctggcatttt attatataaa attgttataa
       61 aaaatttttt ttgaaaaata gtattgcaaa ataaagcaat ttcatagata ataggtatcg
      121 taaagttata taactttata atacctatta gagtgaggga taaagaaatg agacaactac
      181 acaaagaaca gattgaaaca gcaatggcag cgttaaagga aaagacaatt gatcatgtat
      241 attttacagc atgtgggggt tcacaagccg ttttaatggc tggtcaatat ctatttgata
      301 aagaattaac tgtaccttgt catgtttata ctgctaacga atttaactat gctataccaa
      361 aaggattatc tgaaaattct ttagttatca cttgttccca ttctggcaca acaccagaaa
      421 ctgtagaagc aacaaaaaaa gcaagagaat ttggagcaat gactatttgt ttatctaatg
      481 aagaaggatc tcctctatgg gaagcagctg aatatcctgt tcactatgat tggggtaaag
      541 atgttgatgc tagtgattta aataaaggaa ttctttatgg tttattattt aatattttac
      601 ttgttttaga tggctctaaa aaatgggaaa cttgcttgaa tgaatttgat aagttaaatg
      661 atcttgttat tgctgaaaag agcaaatttg aaataactgc taaagaatgg ggtaaaaacc
      721 aaaaacgaga taaaattatt tacacaattg gtagcggtat taattacgga gaaatttact
      781 ccacagcagc ttgctggttc atggaaatgc aatggattaa ttctaatgct attcattctg
      841 gggaattttt ccatggtcct tttgaaatca ctgattatga tgtaccattc atcttagtga
      901 aaagtattgg agatacaaga tttttagatc aacgggtaga agacttcact aaaaaattca
      961 gtaataaatt aatggtttta gaccaagctg attttgactt agaatctata gatagtgtag
     1021 cgaaagaata tattgcagct gtacttagtg gcgttgttat tcgccaaatg gtagaagcga
     1081 ttgcttttga aagaggccat tccttagatg ttagacgcta tatgtggcaa atgaaatatt
     1141 aattaatagg aggttgcaaa agatggcaaa tcaattattt cacattgtga ttacaacaca
     1201 ttctaccctt tgtgagggtt atttaaaggc aacaaaatta attttaatga atgacgccac
     1261 aggtattagt gctgttccat ttaaagaaaa tactagtcca gatgattttg aggagagtat
     1321 gaaagaaata attagtcagc atcatttgca acctctaatt attttaacgg atttaattgg
     1381 aggaacacct aataatgttt ctattaaaca gttgaacaac aatagaattc aaattattgc
     1441 tgggattaat ttacctcttt tactggagtt attaatggcc cagtcagctg gtaaatctat
     1501 agaagaaata gattttaagg aattactgaa taactctcaa aaaagtatgg ttcatattaa
     1561 ttcgctttta aaagatgtca gttaaggtgg caatttctat tctataaatt ttaaaaattg
     1621 aaggaggaaa tagaatgatt aaattagtgc gtgtagacca cagattactt catggacaag
     1681 taatattttc ttggactaaa cagtatgata ttaaccatat tattgtggct gataacaaag
     1741 ttccaggaga tccaatgtct gtgatggctt tgacaattgc taaacctgct gattgcaaat
     1801 tagacattgt taaaattagt gatgttaaag ggattatgga aacatcccca aatgataatt
     1861 acatgatttt aattaaagga cctaaggaag cttttgaatt agttcaaaat ttaccagaaa
     1921 ttaaagaaat taattttggc ggtgtgtcta agaaaagcga gtccaaacaa tatggaaagg
     1981 cagtcttttt aactcctgaa gaattagaag ccacaaaaaa aattcttgat caagaagtga
     2041 aaatatttgt gcaacaagtc ccatcttcaa gtattgaaaa tgtagatttt agtaagtaag
     2101 gggggaaaat aaatgttatt aaaagccatt ttattaggtt tagttggtgt ggtagctgtt
     2161 attgactcca ggttaattgg tcgacaaaat attggtagac cactaatttt gagtacactt
     2221 gccggacttg tgctaggtga cttgcataca gggattgttc ttggggcatc tttagaatta
     2281 atttctatgg gattcgtctc aattggggcg gctggacctc ctaatatgca acttggtagt
     2341 attattgcca ctgcttttgc aattttaact aatagtagta cagaaacagc cttaacaata
     2401 gctatcccag tggctgtagc tggtgaattt atgagtatta tcatgcgtat gataattgct
     2461 caatttggac atgctgcaga taaagccatt gaaaataata aatttagaaa agctcaaatt
     2521 attcatattt attggtcatt tatttttaat gcttttgttt actttattcc aatcttttta
     2581 acagtatatt ttggtgctga cgttgtggca gatttagtag ataaaattcc agaaatgatt
     2641 acagacgcct tgactgtctc tggaaatatg ctttccgcat tagggtttgc aatgctatta
     2701 agcacaatgt tgagtaaaaa attatatcct tactttattt ttggattctt tattgttgcc
     2761 tattcaggtc tttcactaat tggcgtgaca atttttgcag ctctgattgc ctttgtaatg
     2821 gatcaagtta aatatggaaa aagagaagag gcgcatggtt aatggaaaat agtacagaaa
     2881 aaaaacgttt aatcacaaaa aaagatcttc ataagatgtg tcttcgttca tggacaatca
     2941 tgtgttcgtg gaattatgaa agacaaatgc atatgggatt tatgtatggg atggcgccaa
     3001 ttttaaataa agtttataaa aatgatgaag agaaaaaaag agaagcttat actcgtcata
     3061 tggcattttt taactgtacg cctcaaatga ctccatttat tatgggactt tgtgcttcta
     3121 tggaagaaca aaatgctcaa gcagaagaag gaaactttaa tactgaatct atctccatga
     3181 ttaaaaccag tttaatgggt ccttttgctg gaattggtga ttcatttttc caaggtactt
     3241 taagaattat tacatttggg attggattgt cttttgctaa gcaaggtagt attttaggac
     3301 cgattttagc tgttggcctt tttgctatac cttctatttt agctgttttt tacggcggta
     3361 aattaggata tgagtctggc aataaatacc ttactaaatt gtatcaagaa ggtcttatgg
     3421 atcgagtaat gcaatttgct tctatcattg gtttggctgt agtaggtgga atggtggcta
     3481 gcatggtgcc aattgtcacc cctttaaaat tcactacagg gggaacagaa cttgttttac
     3541 aagacttact tgattccatt attcctggat tattaccact tgtatgtact ttgggaatat
     3601 ataaattgat taagaaaaat gttagtacaa atatgctctt attaggttgt attttggcag
     3661 ggtttgtttt aagtataata ggtatcttat aatgattaag gctctaattt ttgatatgga
     3721 cggagtttta attgatagcg agattatgta tcaaaagtgg gtatttaatt ttttaaaaca
     3781 agaaaattta agctatcctt tgtattctta tcaaaaaaag attggaacta cagcaagtat
     3841 ttttgatgat atagaaaagt acaatgaggg atgtaacagt ttagaattga agggaaaatt
     3901 tactaaatat tggaaatcgc aaaagattga ttacttggat atttttccta atatactaat
     3961 tgaacagctt gaatggttaa aaaagaaaaa aataaaaatg gcaattgctt cttccagtcc
     4021 aatggatact ataaatgaag ttgttaccac ttgtaaaata gagtcatatt ttgattgttt
     4081 aattagcgga agagatttgc cagaaagtaa acctaatcct actatattcc ttaaagcttc
     4141 agaacagctc agaattccag ttgaagaatg tctggtgata gaagattctt ataatggtat
     4201 aaaagctggt aaaagagcaa atatgaaggt tcttgcaatt aaagataaac gtttttctca
     4261 agatgtaagt ctagcagatg atgttattta cgatattaaa tatctgcgac aagaagtaca
     4321 ggtgaaattt ggaatataaa taagtttttt gatgtattta gagaatatta gtaaaaataa
     4381 aaatcatgcc caaatctaat caaaattgat tgctttgggt atgatttctt ttaatatcaa
     4441 tctattactt taggatctga atcattttta tgcaccgtat ctaaagtgat attttctgtg
     4501 acttcaataa cataactaat tctatcacaa cgaactgtgg attgagaata atgaaatggt
     4561 tgattatttt tatctttgcc aaccttttct aaaataaata gcggatctcc tactatacat
     4621 tgcaataagt cggaaagctc aggtgttgca gataaaccgt ttatttccat aatagagctc
     4681 gtagctttga catcatagtc atcttctaga agtttatata atgaagtata ttggctacct
     4741 ttttcaaata aagttggaaa tttttcttct gatgcgaaaa aattatcaat tcccatcggg
     4801 gcaccgtctt cccacattaa acgtttaata ttgtaaactc tatcagttac attgatttgt
     4861 aatttattag ctaaaaactc atcagccttt ataattttag aatgtaaaac agtatatcta
     4921 attttatgtc ctcgggtaga aagagaatcg ctaaatccat gaactccagc taattttaaa
     4981 ctttcttttt tttgagttac aaaggtccct ttccccctta atcgttcaac tgttccactt
     5041 tctgttaaaa gtttaaaagc ttttcgaatg gtaatcttac ttactccata aattttacat
     5101 aattcttctt ctgtaggtaa ttgctctcct ggaagatatt ttttttcacg aatatcgtta
     5161 cgtatagaag cttctacttg tagatataat ggtctattat cttgaaaatt catagtacac
     5221 tccctaaaaa gttatacttc attatatcac aatttattaa aataaaagat taagatataa
     5281 atataaatga actagtcttt gactaaccaa tgcccgcttt tgaaagaaaa caaattcatt
     5341 ctgttttaag taaaaatcct tatgttacca ctcattctga aggggatgag ccttatcgct
     5401 acttggttgt ggaaccaagc gataagacaa ttttataaat tcaatattaa ttaaaataaa
     5461 aacaaacaaa gtagattatt ttactttgtt tgttttttta t
//
LOCUS       DAFEOM010000117         5482 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.124, whole genome shotgun sequence.
ACCESSION   DAFEOM010000117
VERSION     DAFEOM010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.124"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(126..772)
                     /locus_tag="LP524_002705"
                     /pseudo
     CDS             complement(126..772)
                     /locus_tag="LP524_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(1180..1683)
                     /locus_tag="LP524_002706"
     CDS             complement(1180..1683)
                     /locus_tag="LP524_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL2235693.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(1806..2570)
                     /locus_tag="LP524_002707"
     CDS             complement(1806..2570)
                     /locus_tag="LP524_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBL2235694.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            2678..2953
                     /locus_tag="LP524_002708"
     CDS             2678..2953
                     /locus_tag="LP524_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBL2235695.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            3391..4341
                     /gene="yidC"
                     /locus_tag="LP524_002709"
     CDS             3391..4341
                     /gene="yidC"
                     /locus_tag="LP524_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBL2235696.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(4520..4720)
                     /locus_tag="LP524_002710"
     CDS             complement(4520..4720)
                     /locus_tag="LP524_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235697.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
BASE COUNT         1610 a         1141 c          952 g         1779 t
ORIGIN      
        1 aaaggggctg tccccagact ttagtaggcg tcattagcta tctttcgtta gcatttaaag
       61 gcgaacacca tcacctattc acttccatta aaagatagca tatttttctg taaaaaacaa
      121 taaaattatc gttctttctt tacacgttca gcaaattcat agaaaggagc caatttcgtt
      181 aactgttgat tacattggtc actatcatga caaatataat tccccttttt cgtatacgtg
      241 ccatcagaac ctgctttcgt tgtagaaaga aaaagcgaaa cattcgatgt tttctgacag
      301 attgcacaga ttcccttatt tatcgttggt gataaagtac cgctaatccc ttttaatttt
      361 tcatcttcgt aatagaccat gaattttttc tgcgtacctg gatcattcca gccgatgtac
      421 gtatgttctt gcaagtcgat ttcattccaa gctggttgtt tgagtttttt cacttttcgg
      481 aataatttct cgatttgtgg tttgctaggc tgcataaacg gctgaacgat ttgcttcagc
      541 tcctccaatg ctctttctgc ttgcgctttt gtcagtttta cgtctaaaac tgtcgttaaa
      601 aatgtatcga ttctggcagt tcgtgatcta atatgtctcg aatcttttca gctgctagtg
      661 cctgaactgt ttgtaccgtt tttggatcat tgacagatga gtacgcattt aataactgtt
      721 ctacttctct tattataaaa aaatattggt atggttttat tgtttgttcc atgattttaa
      781 agtcctttct tctaaccaaa gtgcatactt tgtagaaaaa agtgatcttc tgcaaagtat
      841 gtgttacctg aagatcccgg actcttttaa ataagatacg ttttgcatct tatcatcacc
      901 ccctttaatg cttattattc tatgtacatg attcattcga taaataaaag taaaccgtat
      961 cttgaacaga tagtcaactg caaacagaaa aaaggactgt gacaaaagtc gtgtcaaagt
     1021 cccttgtttc gaatgaatgg tgttcaaaag ctggcatctg cggtaataag ccgaaacttc
     1081 acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc
     1141 tgtcacaacc tcaatatggt ctattattgg ttttatctat taaatcgagg attctttccc
     1201 aaagtaacgt tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac
     1261 tgcgcagtat ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat
     1321 cgttgcgccc cacatgtgct tcaaaatgat atctcgctca agatccgaca attcggtgat
     1381 cttttctgca ttctttacag caattcttgg atggatatac gcatgtgtgc cttcatcaaa
     1441 tttcgtcgta cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc
     1501 tttagcattg ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga
     1561 atgctccaaa cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc
     1621 tgtttctaaa agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt
     1681 cataattatc gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat
     1741 accaccagtg ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc
     1801 cttaatcata aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat
     1861 tccgcatttc ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga
     1921 cttaccccct gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg
     1981 tctaatgcgt gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc
     2041 attaaaggaa tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc
     2101 tgcatgctgc gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca
     2161 ccagccatat caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt
     2221 acattatcta acaacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc
     2281 atcactgcta agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc
     2341 gaaactagaa aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg
     2401 cctcgctgtg tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc
     2461 agatggaacc cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt
     2521 ttttttaatt ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc
     2581 tccccatgat tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta
     2641 tcattaattt ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt
     2701 ccaaggacgc gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact
     2761 aggcgtgact ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg
     2821 caatgacgac atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg
     2881 acgagtgcag tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga
     2941 tgtggtggga taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga
     3001 aaaatagctc tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga
     3061 acaccgtcga taaggttatt cttgaggcgc tttgcctcaa aaattaagga cagcagtgaa
     3121 aaagctgatc tatcacttgc agtgagttca tttgactccg ctttttcggg cagaaaaaaa
     3181 gtttttagtt tattaaagta agaaagaaac acttggaatc attctacttt ttcctagtat
     3241 ttctttcttg attttttgtc tttttttcaa aatttttcgc tacgtaaaag aacgaatatt
     3301 tatatgaaat ttctatgaag ttgattgaat tccatgcgct tttttagtat agttaagttt
     3361 gtgtaaaaat tatagaagag gaagtattct atgaataaat ggaaaaactg gttattggga
     3421 tccggacttg tcagtatcct gctcttttta tctggctgtg tgcggatgga ttcaaacgga
     3481 aaccctgaca cttcgggaat cgtttatcga gtactcgtcc atcctatggg ccaagcgatt
     3541 acatatctag ttgaaaattt caattggtct tatggctggg cagttattgc aatgactgtg
     3601 atcgtccgta tcatcatctt gccactaggt attagtcaat cgaaaaaaac aatgatccaa
     3661 tctgaaaaaa tgcaagcgtt aaaaccacaa gttgaagcag cccaacaaaa attgaaagct
     3721 gcaacaacac gtgaagaaca aatggcagca caagcagaaa tgcagcaagt ataccgcgaa
     3781 aacggattga gtatgaccgg cggaatcggc tgtctgcctc tattgatcca aatgccgatc
     3841 ttctctgcct tgtactttac ggcacgttat acagaaggta tccgggaatc atctttctac
     3901 ggcatcgatt taggctcacc aagtatggta cttgttgcca ttgcaggggt cgcttatctg
     3961 cttcaaggat acatctcaac gatcggtatt cctgaagaac agaaaaagac gatgcgtacg
     4021 atgctgatcg tctctcctgc tatgatcgtc ttcatgtcga tcagtgcgcc agccggtgtt
     4081 acactttact gggtagtcgg tggtgtgttc agctgtctgc aaacattcat cacaaacgtt
     4141 atgatgaaac cacgtttgaa agcacaagtg gcagaagagt tgaaacaaaa cccaccaaaa
     4201 caagtcgtca ctccaagaat tgatgtgaca gaatcagcta aaccagctga cagcaaacaa
     4261 gcaaccaaac aaatcaagca acccaaaaac agtacaaaca ataaaggccg caacgcagga
     4321 aagcagcaga agcggaagta gatagtaaat aagagtgtaa taaaaattca ttaacagttt
     4381 tagcaattaa aaatatcgac caagattttt gaaaaagaaa tctggtcgat atttttattt
     4441 aagtgaatgt ttaatttgaa tcatcgattg atcgctatct aattgcagca gcatcttttt
     4501 atgaagaaac aactttttct tatctatcaa cgtagacagg tgttgctgtt tcttctaata
     4561 gtgtacttaa ttccttaata ttttgttttc cttcttctaa catccaacta ctcgcttttt
     4621 cttcgccttc ggtaacaaaa ggcgccactc ccttcgccca tgttgctcgc ccacataaga
     4681 caccattctc cataaaggac aacgcggaga taccacccat ctaaataaca tccataaacc
     4741 tttctagtag aagaatagtg atagtatcca tccctttttc tactgggaat acaattacac
     4801 gattcaaagc ttgatacaca acatttccct tcttataaac cgctattgta accgaattgt
     4861 tttgaatcac ctttttggga tagaaactgg ctcctttccg caacttctta aatgttggtt
     4921 tcttttatac tttctttgta gcaactacaa atttcttcaa atctctaaat gtttgcataa
     4981 caaccgcctt tctgactttt actcaggatg gtatttaaaa atcagcaaaa taaggcttct
     5041 ctccatcttt tttatttcct agctgcttta aagacactct tgctgttttt taatctctat
     5101 caagggggct ctgaatctat tttgatgaac ttatcttatt aatttctcaa atgagctgtc
     5161 agacacaact gtattttttc aatctcatac cggacaacta tgccagctta aaaaaatttc
     5221 ctctggcaaa acttctttat tgaaaaatga caatgaaaaa agaccattct ttttgttgaa
     5281 tgctcattgt gtaaacatat ttcctgcgac tgctcttcga accattctca gtgcaatccc
     5341 taatttatct attaaatctt cttttagttc ctcatctttc atattcaaaa tttcctttgt
     5401 atgttacaca agtttgttct ctaaataacg cgaattgtaa aattaagtta gaaaaataaa
     5461 aaggcattta tggtacactc aa
//
LOCUS       DAFEOM010000118         5480 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.125, whole genome shotgun sequence.
ACCESSION   DAFEOM010000118
VERSION     DAFEOM010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.125"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(72..542)
                     /locus_tag="LP524_002711"
     CDS             complement(72..542)
                     /locus_tag="LP524_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBL2235698.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            complement(539..781)
                     /locus_tag="LP524_002712"
     CDS             complement(539..781)
                     /locus_tag="LP524_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBL2235699.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            complement(1327..1857)
                     /locus_tag="LP524_002713"
     CDS             complement(1327..1857)
                     /locus_tag="LP524_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL2235700.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(2039..2695)
                     /locus_tag="LP524_002714"
     CDS             complement(2039..2695)
                     /locus_tag="LP524_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HBL2235701.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(2822..3295)
                     /gene="dfrF"
                     /locus_tag="LP524_002715"
     CDS             complement(2822..3295)
                     /gene="dfrF"
                     /locus_tag="LP524_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HBL2235702.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(3714..3932)
                     /locus_tag="LP524_002716"
                     /pseudo
     CDS             complement(3714..3932)
                     /locus_tag="LP524_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3963..5177)
                     /locus_tag="LP524_002717"
     CDS             complement(3963..5177)
                     /locus_tag="LP524_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HBL2235703.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1711 a         1130 c          776 g         1863 t
ORIGIN      
        1 taaaaatctg tatagaaaaa gcagtcaatc ctaagactaa ctgctttgtg tgtatggata
       61 taaaattgct tctaatcttg aataggtatc caaatttcaa taactgaatt atcactattc
      121 caatgaaaac gataatcgta tttttcaaag tgtaggataa tatcttttat aggaatatac
      181 gatgtactag gaataatttc ttcataaatc ttgccataag tttcatatat tttatccata
      241 gcaccaatat gctccacaac caaatattta taagacggta tttctttata cagaaagccc
      301 tctggggtaa tggttctttg gggaatagaa cagaaataat aaagttttcc atttcttctt
      361 tccataaaag catattttac ccaatttcct gattgtgaaa gatacgcttt tttcaaacta
      421 ctgttaaact ttctccaaaa ctgtgtacta atctgaatat tctttttttg ataattagtc
      481 agttctactt cctgcccaat aactgcaaat gcgtttaatt cctttataga aaaatttatc
      541 attttacctt gtgagcagtg ataacggtat aactgtacgc atttaccgtt ataatacaat
      601 tatcaatcgt tatataccag ttttttcctt ttcttgtgat ttccgcatta gaaaggctaa
      661 ttttttcttt gcaccattca attacatttt ctacattcaa agataagttc tttttaattc
      721 taataacgcc aagttcagtt gtgtgaagtc ttcctaagtt ttccagtaat tcattttcca
      781 tattttgata cctcattttt cagcaaattt atttgtatgt tttatacccc aattataaca
      841 ttctcatatc agcactacaa taaaaataat taagcaccaa taatcttatt gaacatctcc
      901 aaccgtaaat ctttcacacc atcagtgata aacactgagc tgacagcaat tacgacaact
      961 accataattg cctgcactta aatttctgca aaagaaaacg acaatcaatt tcttattttt
     1021 tgaaatcaac tgtctttctc atatttacta ttcaattttc atagtgatat aggtacaatt
     1081 tatatcacta ctgtaaaaat ggggttttgc cttataaata cagcgttttc cgcttgttag
     1141 tgtatagtgt ttcttgtttt ttgtgccccc cttgttagat aacgggggct ttgaacggct
     1201 cgcaagctcg ccgaaccgcc gaagcgaacc gcccccttgc aagcatggcg gaaaaatctt
     1261 aaggattttc ccgccaacgt ggtcggatcg ctccgtcggt ttaacagctt ttctgtttct
     1321 ctctagttat ctacgcatat aaatcaaatc ggttattccg ttataagtct gtgttttaca
     1381 aagccttaac tttatttccc tgtctatgtt gcccaaaaga cgaaccccat ttcctaacag
     1441 agtaggaata acagaaatgt aatatttatc aatcatattt ttgcttacca actgctgaac
     1501 aagatttgct ccgccacaaa tccaaatatc ctttcctgct tcttgtttta gccgttccaa
     1561 taataaaaca ggattttcac ttgtaaaatg aatttgttgg gaagaattgc actctttatg
     1621 tgttaataca taagttgtaa atttattata tacccattct tgcggagaaa gttcagtaac
     1681 aacctgatga gaagtattcc accccattaa gacagtatct atatctttgg taaattctgt
     1741 ataagtgtcg atgttttcat tgtcatttcc atgaccattt agccaagcta ctccaccatt
     1801 accgtccgca atatatccgt caaggctcat ggcaataaat aaaactactt ttctcatttt
     1861 ttctctttta taaatacaaa caggaattta tttctctttt ttactcataa gggaacaact
     1921 gattgctaca aataaaccca ttaaaatcca tggaaatgct gctcttataa aatcactaac
     1981 catactttat tcctccttaa attccaattt ccttagttct acaaactgaa atttttgcct
     2041 atgaatgtat cttgttcttt atacgcaaac aggcttctgt gtgcataatc caatgtcttg
     2101 aaaacggcat ttgaattagt ccacaaatat ctttattaca ccaataatca atcagccaaa
     2161 ttgctttttc attgtcgttt actacattca acgattctaa gtatcctttt ctttctttcg
     2221 gtatttttct tttcaattca tcataagata accgttcaag cattttctca gtgctttccc
     2281 atacttcgaa aatatataaa tataattctt ccagattaag ctgttttgaa aagtctgcaa
     2341 tctgctgttt catgagttca tttcctgttg taatgatagg tgaattgata cgctcttgat
     2401 aattgcctgc aaaaaatact tgttcatctt catttatcac tgtatgcaca actatatctt
     2461 caatgcggaa aatatgccaa atagaataag caatcgtctt actatgataa ccatcagcgt
     2521 ttatgaaggg gattgagtta aaatcttctc tgcataattc ttctttgaaa gatactaagg
     2581 tttgtattaa ctgacttcgt aaagtaagta aagtatcaat acccctctta taagtatctt
     2641 tcttttttat ttgtgcctgc attgttttat taagttcaga ccattcctta ttcatattca
     2701 atcctctctc aaattctgat tttatcgccc catacaaact ggaatttgtc ttacaacaat
     2761 ttatatatgc ataaaaagca atatatttat gaaaacaccc taaatccaaa atttactcaa
     2821 ttcatttttt ccttacataa aatgttctcg tatatttcac ttcctcacca agtgtttccc
     2881 ctatcaaaac ttcaaaatca ttgatatcaa attctggaaa gaatgtatct ccatcttcaa
     2941 tatttaaatc tacttctgtg atatacattt tatctactat ttgcaaagct tccttaaata
     3001 gtccatatcc accagatatg tatacatctc gtcctttagc caataataat gcatcttcta
     3061 atgatttaac tgaaactaaa ttatctcctt gatactctgt tgtggtggaa acaacaatat
     3121 tcattctatt aggcaacgga tgaccgattt cttcataaga ctttcgcccc ataataacca
     3181 cattacccgt tgttaactct ctaaattgct tttgttctcc ctttattttc catggtatat
     3241 taccattctt gcctataaca ttattctttg acctcgcaac aatcaaacct atcatctatt
     3301 tcatcctcca taatatgatg tcgtacctgt gattccatca agtgcaccac atcttaacaa
     3361 actcaaaatt atctcgaact aaccagctcg acaaattcct tttttgtctt catctgcatt
     3421 atatcatatt gttataagca ttgccatttc ttttctgctt ttgaaaagtt acttatcagc
     3481 ctatgttttg taaagggtga tattcagcat ttctcacgca acaagccctt gacaaaccat
     3541 agactaataa gttttaaggt aatcaagcag aaatgaaata gtctgctgtt tcacggcaac
     3601 aaaaaagaga taccaacaca cttatgtagt atctcttagt ctagtagcaa tattcttttg
     3661 tttgcaagca ggtaaacatg ggctgtttct taatatcttt ttgtttggga aacacctttt
     3721 tcccatcctt tcaccgttgc catctctgct gcgcacaatg ccacagggat aaaccatagc
     3781 aaaccgccta ataacaagaa aaatactaag ctgaagcctg aggtcgcgaa tgtgggatat
     3841 tcatataatg aaacgaccat tgagatggtc atagccaaaa agccaaagaa cgttaatttt
     3901 tttgcaggaa ttttcgtttt gctttgttgc atgaatcttc ctcttttcta tttgaatttg
     3961 tttcaattga gtttgttgcc aaagaagaaa aagtccaatc cttcttcttg ttcttctttt
     4021 gtagtagtga tcgttgtaaa gtgcatcgcc gattttaaaa tcactgttgt cagcacatag
     4081 gcatacacgg cagtaatgat gacaccaatc acttgaatcc ctaagaagta ccagctgctt
     4141 ggattcgtta gtaaatggct gttagatgca aagatcccta tcagaatcgt acctgtgaat
     4201 ccgcccatgc catgaacacc ccacacatca agtgcatcat cccattgttt ctttttacga
     4261 aaatcaacag cagcatgaca gacgataccc gcaatgatcc caatcagcat ggcacttttt
     4321 gcttctacat aaccggcaca aggcgtaatg gctgccaatc cagcaacgga tcctgttagt
     4381 acgtcgacaa aatcaaagcc atttcctttt acttttgcaa tgatcagcca agtaatcatc
     4441 gcaaatgcaa gagcgatgaa tgttgaacca aatgcattga ctgcttgttc atctgctcga
     4501 agtgcaccac cggaattaaa accgaaccag ccaaaccaaa gcagtcctgt accgatagca
     4561 gctgccataa gactgctatg ctgacttctt tctcctaaag gaatctttct ttgaccaagt
     4621 gtgaggatac atgctaaaga gccgaatcca gctgtcgtgt ggatgactgc tccaccagcg
     4681 aaatctctaa atcccatctg atccaaaaat ccgccacccc atatccagtg gcaaacaggg
     4741 aaataaatca gcagattcca aaaaataaca agtataatat agccttttaa attcaatctc
     4801 cctgcaaaag ctcctgtcat caaaggaacg gtaatcacga taaacatcag ttggtaaaga
     4861 aagaacatta aaaatggaat cgttgcacca tgaaacatgt tggggaagct tgtaacatgt
     4921 ctcatcaaaa agaaatctgc cgggtttcca ataacaccgc cgatatcccg tccaaaggcc
     4981 aatccgaatc caccaaatac ccacataatc gttgttatcc caacggagag aaaagattga
     5041 gacatgatcg ttagtatatt attcttccgt gctaatccgc cgtaaaaaaa agctagacca
     5101 ggagtcatca ggcaaaccat tgctgtacat aaaatcatga atgctgtatc tcctgtattg
     5161 ataggtaatg tattcatttt tttcgcctcc tttctacgat tacgagtata aaagaaggtg
     5221 tagttgtttt tttcttctgt ttttttgaat taatacttgc atttataaaa gacagacaat
     5281 taataaaagc ttgatgtgtt gtgctctttt tcgtttttca cacaaataaa gacaaaaaat
     5341 aaacggtctt ctttttatag aaaaccgttt attttcgtct ttttttattt ttaaagatat
     5401 tttttcataa ggaataaaga atacttcata taaaatatat aaaaaagaac cgcagcgagt
     5461 gtcgctacgg ttctcaaatt
//
LOCUS       DAFEOM010000119         5051 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.126, whole genome shotgun sequence.
ACCESSION   DAFEOM010000119
VERSION     DAFEOM010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.126"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(139..810)
                     /locus_tag="LP524_002718"
     CDS             complement(139..810)
                     /locus_tag="LP524_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBL2235704.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFRLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            complement(892..2058)
                     /locus_tag="LP524_002719"
     CDS             complement(892..2058)
                     /locus_tag="LP524_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HBL2235705.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTEFTVFDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFVILEILPKKIFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFALSMILLLSFLWLL
                     VRKCELTNGQLTFLWAFLAIMCQSFMEETLATISWVPIVFLMLAMATHQYKTTR"
     gene            complement(2064..2753)
                     /locus_tag="LP524_002720"
     CDS             complement(2064..2753)
                     /locus_tag="LP524_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HBL2235706.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDDVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYPDAEMGNGNYILAGYNVDTPSVLLSDMIDLSEGEEIDLIDA
                     HYKYQYEVSKRGKIYDYSQVVQSKENNSFLSLPKAGKKPLLTLLDFEANNMKKCDVIQ
                     CELVNVEKINK"
     gene            complement(2880..3119)
                     /locus_tag="LP524_002721"
     CDS             complement(2880..3119)
                     /locus_tag="LP524_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235707.1"
                     /translation="MNCKESVNWKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMQIT
                     GKEKACLLGSMKNFEQLRRTYVKTSSNCQLLLEVS"
     gene            complement(3186..3425)
                     /locus_tag="LP524_002722"
     CDS             complement(3186..3425)
                     /locus_tag="LP524_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235708.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            complement(3539..3880)
                     /locus_tag="LP524_002723"
     CDS             complement(3539..3880)
                     /locus_tag="LP524_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HBL2235709.1"
                     /translation="MKNYDPNIRWGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEEDLYDQKLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            complement(4500..4832)
                     /locus_tag="LP524_002724"
     CDS             complement(4500..4832)
                     /locus_tag="LP524_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBL2235710.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQNKTVVQCKKYI
                     IQKDKVSEEMIYYVGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
BASE COUNT         1548 a         1052 c          705 g         1746 t
ORIGIN      
        1 tgcttgatac ccctgaaagc agcttgcaat gattcctaac attccaacaa gcattccagc
       61 tttttttaca tatcgattta ttgccatttt tactctcctc atttcatatt tcaacgactc
      121 gcgttgatta aaaccttgtt attgtagctc tccctgtacc acgtatcttt cttttccttg
      181 tgacgtatag acacaagtaa acaacgttaa taagggtttc tctcctttct ttggcaaaga
      241 caagaaagag ttctcttcag gaacgccatc gattaatcta aagtacccag gtacgataaa
      301 tttacgcgtt accttgtaac gatagacgcc atcgttacta actaagtcca tcacctcccc
      361 taatgacaac ttttgaatat cagaaaataa aacacctggc atttccatgt tatgacctgc
      421 taggacatag tttcctttgc ccatttcagc atcttcataa aaataagtag ccgctccaag
      481 actcaaagtg tattgatttg cccctttaaa aatcggcaat gctaaaccaa gtgcaggaat
      541 ctgaatttgt gcaaatactt gtttattgac gcccatttcg attgcttctt tttgtatgga
      601 caacatttgc cccaaagaca tatcattatt gacgttcgtt gcatcctcct tataactctt
      661 tgcatgtgca ttgatttgat ctacgggcat ctttttttct atattttgct taaacacctc
      721 tccttcatgt ttttggtagc taacatagcc aaacacactg caaacgacga ttaaagcgac
      781 tcctaaaaaa ccaaataacc attttttcat ccatctgcct cctttcagac aaaacaaaaa
      841 acactttttc acaacgaaaa agtgttttct cttattacta ttctatttca tttatctggt
      901 ggttttatat tggtgtgtag ccattgctaa cattaagaac acaattggta cccaactgat
      961 tgtagctaat gtttcttcca tgaaagattg acacatgatc gctaaaaaag cccacaagaa
     1021 tgttagttgt ccatttgtca gctcacattt tcttacgagc aaccataaga aactaagcaa
     1081 aagaatcatc gatagagcaa aaccaatcaa tccatagtga ttgacgagtg cgtgatacgt
     1141 attatggtta cctagaatct gatttcctct atggaaaaag aaaggtttca tccctacaaa
     1201 tatttgtcct gttttttcgc ctaatgtttc atagaatttg cgccaaattt cttctcgtcc
     1261 tgtaaataaa tgaactttgt ctgacattgc agcaaaataa gaaatcggta aagctagaac
     1321 cgaaactgct gcatacatcc aaaaggacaa gtgtgtgttt cgaaagatct tcttaggtaa
     1381 aatttctagg ataacgaaga taacaagcgc gtataatgct gctttcgatt ggcaaaccca
     1441 aatcgttgcc ccagcaagaa tcaacgctgg taatgatagc aatcgcatgt accattttcc
     1501 aaatgaacta gcaaatcctg tgataagaag ggctaatgtc attaaagaac tacctatcgt
     1561 atttgtattg atccaaatat ctgataattt gttcgatgga ttccaaatac tttgtccttc
     1621 tggtactggt atctcggtaa atactcgata aaccgttacg agtaaaaaga catagaatac
     1681 tacaagatac aagctacgat caaatacagt aaattccgtc cattttacaa tcaaaataat
     1741 ggcaataatt gtcggaacga tcagatcacc atagaaagta tctcctcggt agcttctaaa
     1801 aaaagaaaag aaaaggtaaa aaagaatgcc tgctgcaatg aagaatagat cgtttctctt
     1861 taccttgtac agattcaaaa gaaacaaaca gcctacaaaa aacgtcacta actgtgcagc
     1921 attatcataa ataaataata tcgtaggagg atttaacccc accgtccata ttaaataaag
     1981 aatggatacc aacagtaaca gaaaaatggc actcatttta ttgatcaatt tttctacaag
     2041 ttcttgaatg tgactcactt cccttattta ttgatttttt ctacattgac taattcgcac
     2101 tggatcacat cgcacttttt catattattt gcttcaaagt ctaacagtgt gagtagtggt
     2161 tttttacctg cttttggcaa ggataaaaag gaattgtttt ccttagattg aactacctgg
     2221 ctgtaatcat aaatttttcc tcttttggag acttcatact gatacttgta atgagcatcg
     2281 attagatcaa tttcttctcc ttcggataaa tcaatcatat cggataataa aacgcttggt
     2341 gtatctacgt tataaccagc aagaatataa tttccatttc ccatttcagc atctggataa
     2401 taagtagaag ctactcctaa actaagcgtg tcgctattcg ctccttgaaa aacagggaga
     2461 ataaatcctg tagaagaaaa aacaagtttt ccagcaattt cacctttgag atccttttct
     2521 aatgcctgtt tttgattagt aatcagttgt ctcttagaaa tatccgttaa actggttaaa
     2581 ttatcttgat aggttttagc ctgttcctga atatggattg tttttgttac atcatctact
     2641 ttttcagcga ttttgtcctt ttgataacct tgataagcaa aataactaaa aaggagaaaa
     2701 acaatgatta cgcccacgta aggattaaat aaagttttat tgtttttatt catctatcta
     2761 ccttctatat attaattaat ttacctacaa ctaaccaaga taaaccacgt taaattttac
     2821 tattcttttt attcaataac aatgccacat cataattgat cattttcata tcataatcgt
     2881 caagaaactt ctaataatag ttggcaatta gaacttgttt tcacataagt tcgtctcaac
     2941 tgttcaaaat ttttcattga tcccaataga caggctttct cctttcctgt tatctgcata
     3001 tcattgatat attgcatgac agataatctt ttaaggtgtt tttgtttcat catttttagc
     3061 aacttttctt ccatttcttt tgacactttt ttccaattta cgctttcttt acaattcatg
     3121 agttttccta ccttccctgt tttttgcaaa aataaaaaag cctatgatca catacgctct
     3181 acacttcagt cttgggttat ctttttcttt cgtttaatta ctggccgtgc tggtttcaaa
     3241 tccttgattc cattatgggt tctccaataa tcgaattctt ttcgtaatct ttctttttcc
     3301 atatctgttc ctttagatag ggctttgaat acatctttag gtgaataacc acgagctaac
     3361 agctgtacat accttccttt tgtcaaagta tccatatcca tcgcctctaa aactggtcgc
     3421 gccatctaat tttcctccta aataaattcc ttactcaatg cctaaggtaa tactaccttt
     3481 ttattaatcg tcgtgactcc ttctaagcct ttatctgtaa aatttattcc tccagttttt
     3541 attcttcttt atccgctaca aattcaatga tttcaacgct gacaatataa tcactcaaat
     3601 atgaccaagt atcttcaaca gataattcgt ctccattatc attcataagc gtcattttaa
     3661 accattcatt accttcatca tcttcaggta gcaagccgaa tccaattgga ttatcagtca
     3721 atttttgatc gtataaatct tcctcgtcta atgctaaaac atctaatcct ttacaatttc
     3781 cgcctcttct aaacgtcaca aatcctttgt aatcccaacg ttggaaagaa accttaatag
     3841 tatgtgtgcc ccatcgtata ttcggatcat aatttttcat ttgtcattcc tccattacgt
     3901 aaatttcatt ttctctatgt tttatatcaa ttttttcact ttattcagga ctcgttcata
     3961 ttttatcgtt tttcgtttac ttaaaaatac ataaaaaata tcgactcgtt tatggcgtcc
     4021 gaaacgagaa accctagtag aacatcggta aatgctcgat tgatccaata atcaacgtcc
     4081 tcgcaagtat ctacatgtac tgcagcacac gacacaattt cttgtgcgtc ctccccatta
     4141 cgagaagacc tactccttat tccatgagtt tgcgtcaaag cctagatccc ttagtagctg
     4201 ctcatgatta atctaggcgc tattctaact tgtctaactt ctaaattccc tcttgtttac
     4261 tctgtgcata ttatttacac aaattcattt caccactatc caaacaaggg catttactcg
     4321 attgaccaat tttttacgtt accaatcgta aaaacctacc ccatcactcc ccgaagggaa
     4381 ttgttgatag atcaagtcta ctccttattt ttctagtgat tctaagttct tttagaagtc
     4441 tcgacaatat tttcaagcaa gcgaaaaacg ataaaataca agttataaga atgatcaatt
     4501 cagctgatca aacgccccat cccttttgcg gtacaacgta agactccatt ttttcttcga
     4561 cctatcaata caccgttttg gtcataaaca tgtttgattg ttggatatag ctcttttaaa
     4621 gggataggat cttttaaaat tatttgcttt tgtttcccaa cgtaataaat catttcctct
     4681 gagactttgt ctttttggat tatatatttc ttacactgca caacggtttt attttggcca
     4741 gttgatagtt tcttcgtgat cttctctgta gcggtacacc gtttaccgta gtgacaaata
     4801 tcttcgagac tcaaaattcc tgtatatgac ataaaataat ccacccactt actttaaata
     4861 tttttaacaa gtttttcttg ttatttttca aaaatagaga attgattttc gttcatgaca
     4921 aacaaaaaag agggaacgca tccaaacatg cgttccctct aatttcagcg ttcatcatac
     4981 ggcaaagagc cacacggaca tttttataac attatgctta ggcatagatt catcgtaata
     5041 tcacgtattt t
//
LOCUS       DAFEOM010000120         4994 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.127, whole genome shotgun sequence.
ACCESSION   DAFEOM010000120
VERSION     DAFEOM010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.127"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(<1..563)
                     /locus_tag="LP524_002725"
     CDS             complement(<1..563)
                     /locus_tag="LP524_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HBL2235711.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="LP524_002726"
     CDS             complement(750..1400)
                     /locus_tag="LP524_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235712.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="LP524_002727"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="LP524_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBL2235713.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /locus_tag="LP524_002728"
     CDS             complement(2912..3910)
                     /locus_tag="LP524_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBL2235714.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="LP524_002729"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="LP524_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBL2235715.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
BASE COUNT         1516 a         1066 c          772 g         1640 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatctttggc tctataattt atgggactga
     4921 tgaatcagaa ttgattcttc agccccattt ttattttagg tattaaaaaa catatcgttc
     4981 tctagtatga ttaa
//
LOCUS       DAFEOM010000121         4986 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.128, whole genome shotgun sequence.
ACCESSION   DAFEOM010000121
VERSION     DAFEOM010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.128"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(80..1888)
                     /gene="pepF"
                     /locus_tag="LP524_002730"
     CDS             complement(80..1888)
                     /gene="pepF"
                     /locus_tag="LP524_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBL2235716.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2064..3062)
                     /locus_tag="LP524_002731"
     CDS             complement(2064..3062)
                     /locus_tag="LP524_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HBL2235717.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3288..3941)
                     /locus_tag="LP524_002732"
     CDS             complement(3288..3941)
                     /locus_tag="LP524_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HBL2235718.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="LP524_002733"
     CDS             complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="LP524_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBL2235719.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1480 a         1031 c          762 g         1713 t
ORIGIN      
        1 aaaaaatacg tcgactccct tacaagagag ccgacgtatt tttttcgttt ctattcgaga
       61 aaatcaaaaa tagaaagcct tataaacgat cgatcaattc ttctaattca tttagacgtt
      121 gttcaaacat cgacattgca tcttcaatat atgcagcctg tgtcatatca actcctgctt
      181 ttttcattac ttccacagga taatcgctgt tacctgcttt taggtaagcc aagtagttct
      241 ctagtgcctc tggttcctgg tttagtattt tcttcgccag tgctgaagca gcagaaaagc
      301 cagtcgagta ttggaaaaca tagtaattat aataaaaatg cggtatccgt gaccattcga
      361 atttaatttc tgggtcttct tcgactgctg gaccataata ttttgcattc aatttaccat
      421 aactatcact taggtattca ctagtcaatg gcacaccttt ttcatcttcc gtatgcatga
      481 aatgctcgaa ttcagcgaat tgtgtctggc ggaaaactgt tcctttaaac ccatccaagt
      541 aatggttgag tacataagcc cgtacacgag ggtctttttc tgtttccaat aaatactccg
      601 ttaggatatt ttcattcgtt gtcgaagcaa tttctgctaa aaagatggag tagtcgccgt
      661 acacataagg ctggttcgaa cgagtgaaat aactatgaac actatgtccc atttcgtgga
      721 caagcgtaaa tagctgatcc agcgtatcat gccaattcaa taaaatatat ggatttgtgt
      781 catagcttcc cgaagaataa gcaccgcttc gtttcccttt attttcgaca acatcgatcc
      841 aacgttcaga gaatgctttt tctacgatgg ccatgtattc ttcacccatt ggtttcagtg
      901 cttctaaagc tttttctttt gcttcttcgt acgtaaaggt aattggagct tcccctaaga
      961 ccggtgtata aagatcatac atgtgcagtt tttccacttc taataaacgt ttccgtaatt
     1021 ccatgtatcg atgtaacaaa ggcaaatgct tgtttaccac gtctaccaaa gtatcgtata
     1081 cactttcagg aatatgatta ttgctcaaag acgcttctct ggcagagctg taattacgga
     1141 ctttcgcttt aaaattatgt ccttttatat gtgtgcctaa agtagaagca aatgtatttc
     1201 taaattgttc gtaaacactg tacaacccct taaatgctgc ttcgcgcacc cttcgatccg
     1261 tgctttctag caactgacca tacacgccat gagataattg gactatttca ccattttctc
     1321 cttcaatcgt tggaaaaact agatctgcat tattcaaaac agcaaatgta tcacttgaag
     1381 catcaaagat ttcacctgct ccagcaagaa gagattcctg ctcagcagat aagacatggg
     1441 ctcgattatc tactatttgc tggatataat ggcgatagac ttccaatttt ggttcttctt
     1501 tgaaatattg ccaaatctgg tcatctgaca attgcaatat ttctggttca aaccaggaaa
     1561 cggcttcact aactttcgaa aacagactgc ttgctcttgc ataaagcgct tggtaatctg
     1621 tatttccagt gtcttgatcg tttttaaggt gcgcatatac ataaatgact tcagtttggc
     1681 gataaaccct caatacgaat tcaatcgcat ttaaaaattg agaagcgcct tgatcaagtg
     1741 ttcctttgtg tttttcagat tgttttaact cttctgataa ttccaaatat ttctcatcaa
     1801 actcttggtc gcttgaaaag atcttggtca agtcccaagt taaattttca ggcaattctt
     1861 ctcgttttgg caactgcttt acttccatga aatccactcc ttttcttccc ttagctctat
     1921 ctatcaaaat agatcatagt ctaacatcca catgtcacag catctaactt ttctactcat
     1981 tcatgtgtct gcttccatca attttagcat aactaaatct tgttttacat agaaaaccga
     2041 ttcctagcct atttttatac ttactatttt ttcaaagaag acaagtgtat gcattcgtta
     2101 aaaaactgct gatagaccat ctcttcatca atcataggaa aaagccattc ccttttatgt
     2161 tcttttattt ggcaaagcca tgagttgaaa tcaggaactt tctggttttc caaggcttct
     2221 tcgtacaaca ttcgataaag aaaaacctgt tcttgtaaat aaaagaaaaa ccgacttttt
     2281 tgatagatcc acgatgatag atgcaaaaga tgtttatgac gcaagtagca ttgttcttgt
     2341 atggatactg tattcttttg cttgtgaata agttggcgtg tcagccattt cttcccatct
     2401 tcactcactt tttctatttt tttcatttgg ttcattttaa tttcgttact agtgaatatt
     2461 tctttaaggt ccctactaga aaaagtccag cagctactgc aaaaggaaat atgccctgat
     2521 acagtctctg taatatgatg atacaaataa agcttttgtt tcttgagatc agctttccag
     2581 caatgcactc ctctttttct attatagtag cagcaacttt tttctatcaa tgagaattgc
     2641 gattgtccaa gaaaagaatt accaagtatc caccatactt ggtaactgtt attcaaataa
     2701 ttttgggttc tttcaataaa tctctggtga gacagtctag aacattggat ttctattgct
     2761 agattcgcca acaaaatatc cggcctttgc cgcaacctcg gcaaataggc ttccaaaaat
     2821 actggttcta ctgactgtcc cagccaatcc atgaagcatt cctttaactg taagtgctct
     2881 tttgattctc tttcactaaa gaccgaacag ttatctgatt tttgatgggc aaaatgagca
     2941 cattttttct ttcccttttt caaaatcact ctttttcgac agccacgaca ataatatttc
     3001 tttccttttt ctgcatgttt agcaaaacag cacttattat attcattcat tgcaagcagc
     3061 atttttctcc ctctgaactt atttacgtct aagaatttat ttacacgaaa aaagagggcg
     3121 tgaaaaagct attctgcatc aagtaataag aacagctaaa caaaaatagt tcccaatatt
     3181 tttcgtttgg ttgggcttgt taccgcagat gcaagctttt gaacacattt atacgaaaaa
     3241 aagactgcga cacaattttt gtcacagtct cttactataa tgctgattta tacaaaataa
     3301 aatttcgttc gctcgatagc attacgcgcc atcagacatt gtccatattc attcaatgtt
     3361 tctggtgtac gatcgctctt ttcagcaaac tccaaaattc gagcaatctg attctctaca
     3421 tccgcttggt tgagattttc catccagaag tgaacgacta ggtaatagcg ctcttcgaat
     3481 aaatacaaat cagtccaagc agactctagt tcgacatctg ttgcaagttg gatcacttgt
     3541 tcaaaatctt caaaagcaaa agttgcttgt ctttcctgtt cttctagggc gcttccagat
     3601 aattcttcct ccggatcgct gccaataatc tgctgacgaa gaaagtcact cacatcttct
     3661 gaatcaacag tagaatcttc tgattgagaa atttcttcat ccggaagatt tttactgata
     3721 aacagttcta atccgtctcc ttttggcaaa acttggaaag tgacagcttc gcttcctcgg
     3781 aactcctctt cgatatcaac ttcttccaaa atactataaa agaaattttc gatttctttg
     3841 tgattgccaa gtaaatccaa aaatgaaacg ccccgttcct ctaaatcttc atgaccaatt
     3901 agtacacgga tcgtattttc attaatatgt tccatttcca ttttgctaca cctcactttg
     3961 ccacaatgtt taatatagat tcattgtagc gaaatcagct taaatgtaaa gaaaaaaaat
     4021 ctcgctgtta agcgaattta ataaaaccag cggatttttc aacctttcaa gctatcttat
     4081 ttaactataa catttttggc agacgaatac tatatagccg acttttcagc taattttccc
     4141 tctctatttt tcgtgcaatc atcatcttct actacctttt tatacatcta atcaagaata
     4201 ctttttgtaa aaacaaaaaa acttggaaaa actatctttg taatagtctt ttccaagttt
     4261 cgtattatcc ttctataatc cagccatcaa ttgtgcttga cgtaattcta attgtcgtac
     4321 atcgcgcggt aggaaccgac gaatttcatc ttcattaaaa ccaacttgca gacgtttatc
     4381 atcaatcatg attggacggc gaagcaatcc aggattgttt tgcacaagtt ccaataattc
     4441 ttgtaacggc agatcatcta aatccatgtt cagtttttgg aatactttgg aacgtgtcga
     4501 gatgatttct tctgttccgt cttctgtcat ttgtaaaatc gcttttaatt cagaactgtt
     4561 caatggttct gaaaaaatat tacgttcaac aaatggaatc tgatgttctt gcaaccacgc
     4621 acgagccttt ctgcaggaag tacaacttgg ggaagtatat aatgttaaca acgtgcacca
     4681 ctccttttcg tgataagaat cacttttttg taacagaaga tcataataat ctcccatctg
     4741 tttctattat acataatcaa cttttaaata gctacctttt tttcataatt tttttaaaaa
     4801 aatgaaagtt atctaaggtt aatatacagt aaaaccagca ttctcgcttt ttttatcatt
     4861 taaaaattag agttttcaaa tctaattcaa attttctaaa catatgaaac agcgtataaa
     4921 actgtactat tattttatct caaaagctaa atgaaaccga tttcttaaga ttttttatgt
     4981 ttttta
//
LOCUS       DAFEOM010000122         4794 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.129, whole genome shotgun sequence.
ACCESSION   DAFEOM010000122
VERSION     DAFEOM010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.129"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(207..1169)
                     /locus_tag="LP524_002734"
     CDS             complement(207..1169)
                     /locus_tag="LP524_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL2235720.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="LP524_002735"
     CDS             complement(1171..2610)
                     /locus_tag="LP524_002735"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBL2235721.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="LP524_002736"
     CDS             2795..4741
                     /locus_tag="LP524_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HBL2235722.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1528 a          821 c          790 g         1655 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atag
//
LOCUS       DAFEOM010000123         4679 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.130, whole genome shotgun sequence.
ACCESSION   DAFEOM010000123
VERSION     DAFEOM010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.130"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(91..954)
                     /locus_tag="LP524_002737"
     CDS             complement(91..954)
                     /locus_tag="LP524_002737"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBL2235723.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(1096..3669)
                     /gene="mprF"
                     /locus_tag="LP524_002738"
     CDS             complement(1096..3669)
                     /gene="mprF"
                     /locus_tag="LP524_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBL2235724.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            3810..4616
                     /gene="proC"
                     /locus_tag="LP524_002739"
     CDS             3810..4616
                     /gene="proC"
                     /locus_tag="LP524_002739"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBL2235725.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
BASE COUNT         1462 a         1025 c          809 g         1383 t
ORIGIN      
        1 tcatatctag ccatttcact acaataaaaa actgaagaat accattttag gattcctcag
       61 tttttcatca cctattttac ttcttatatt ttattgcagg gttcgtacta aataaacttc
      121 ctcacaaaaa ccttttaacc cattatatac tctctgcgct cttgaataat gttgacacaa
      181 ggcaaaaacg gttggtccgc ttccactcat caatgcagca tctgctccgt acttgatcat
      241 acgctctttg atttgatgga tgactggatg ccgctttccc gttatatctt ctaaactgtt
      301 tcccattttt ttgatcatct gctgataatc ttgctctcgg atggcttcag caagtccgtg
      361 gatgtcttga tgttggatgg cttgcgtatc tacttctcta aacactgtac gtgtagagac
      421 actcattctt ggtttgacca acacaaccca gcactgtggc atagaaggta atacttggat
      481 ttcttcccct ttgccaccga caaatgccgt acttccgtag acacagtaag gaacatctgt
      541 accgacttcc aaaccgattt ctgccagttc ttctaacgaa cagcccagat tccataattg
      601 gttgatccct cttagagcag ctgcacagtc tgtacttcct ccgcctaatc cagctgctac
      661 tggaatattc ttcttaacaa agatcttgac accggttttg atattgtatt tattttttag
      721 caataaagca gcttgataaa cattgttccg ttcatctacc ggtaaaaatg ctttatttgt
      781 ttctacaatg attttgtctt ctgttatttc ttcaaaaaat agatgatctg ccaagtctac
      841 gctcgacatc accatttcca actcatgata cccatcttgc cgtctatata atacatccag
      901 tcctaaattt atcttggctg gcgctttttc aatcaattcc atcctctcac ccattcgaaa
      961 agtctaagtc gcaagtattc tatcataggg aaaaataaaa gtatagctta tgtctgccta
     1021 gtggttgatt ttgtcatcgt ttcaacagct ttcacagaat tttctcttca taatccaaac
     1081 aagttctagt gattcttata attctgtgag cacttcttct gccaaactcg gaacatttcc
     1141 tttttctttt cctatcagac tgagtagctg catggtagca aatccgaaat tacttccttt
     1201 cctataagca aaataacgtc cttcccaatg atcgacatat tttaatttct tttcataagc
     1261 agactggaag gaatagaaaa aattaccata attatatacg atatgcatga ctttttcttg
     1321 aaagaaagaa aaatctgttt ctccaacatt agctaatgga gctaagccta gattggcttc
     1381 cgagtagccc attttctcgt atacaataaa cagattggct aacagatagt cagctaattc
     1441 ctcagattct ttaggtaaga cccgcaacaa atcatatccc gcctgcttct tcgtcatcgg
     1501 ctgagcggtg atgaagccaa cgactttttg atctttacgg acaataccga caccgctaga
     1561 taatacatat gatttatcaa atcgtcccac agagaagtat ttttctcgtg caccttgaag
     1621 ccatttttgt gaaacttcat ttacagcttg atgtaattct tccggcagtt gatcgtacca
     1681 agtaaaatgg tatccttctt tcatcaaacg ttgatattca agttgctgac tcagccgaga
     1741 caagtcttct gttgctagat caacaatccc tgcttcacct actttcgtga agtgaaaacc
     1801 caattcatga agcatcacta cgtagctttc tgatatctta taaaacgcaa gctgatagcc
     1861 taaacaatct gcttgtttca taaactgatt cgttgctgcc cgcatcattt taggattgcc
     1921 aactggatct cctaaaacaa agcatttgtt tcctttgatc tgataaccaa aaacgacttg
     1981 atcttggtcg tcttcttggt aataaaaata actgtaacct gacaagcgaa gctgatggct
     2041 tgtagctgtg cctccgtatg tttccaataa acggtcaaaa cggtcttttt cgtactcact
     2101 gccaagcttc gtagtatctt cagctaaata ttgataaagc gccactaaag ctagcaatga
     2161 gatgcccaaa ccaataagcc ctgagaacca cacatcatct gaaggaaaca aaataaaatg
     2221 atttcctgtt attcgaccga ctaaaaagcg tccgctgtga tagcctgcaa ttgcgtaaac
     2281 gataaacaaa aagccgaaca agctagcatc aaagattata gctccccaag aataaacgaa
     2341 ttttgttcga taaaattctt ttctggataa ccacacagct aacagaatca agacatatac
     2401 caatatcaac tgccaagata acgtacgtgt gattgtattt aaaataccaa aagatagtaa
     2461 agcaatcgta ggcagaaatg cctttttgac ttttactgaa agtgctctag caaggccgag
     2521 taataaaaag ccaaccaata gattcactgt ctgatctaaa aaattgaatg agaacggcaa
     2581 taatacttga aatagccgac tgattgcaga cagattagtg attgttgata gaagtaccat
     2641 catgatcccc gcaaaataga cagcacttac gagcacgata tgcgcaacct tttggaaaaa
     2701 cagtcgcgga atgttatcta aaaatcgatt gattttgact cctgtatgtg tgatgaacaa
     2761 tagaagtccc gtcataaaag gtagaacata ataaaacaac cgataatata atagccaaac
     2821 tactgctaca tcttgccgaa cagacaattg actcattcct aagatcatca aaacgtcaaa
     2881 agtccccatt ccacctggga ccatcgtcag cattcctatc aatgtagcaa tcatgaacat
     2941 aggatagatc gaaagcatcg aaatatctat tctcatcaac ttgccgatga tcaaaaatac
     3001 cgcaagggct cccgtccatt gtccaaagga tgccagtagc agaccaagca agccttttgg
     3061 ataaaactcg aagaacaatg tctttctctt tagataagca aaaaagaaaa tgactggtgc
     3121 taaaaaacta ccgccgaaaa gccagatcca atagcttcga aaaatacttt ctgactgaat
     3181 aaaaaagaca tcaataaatg taagaaacga ccatacagac aaaccagtca gcataaatag
     3241 tgcgactttt gatacagtgg ctaaaaccat ttttctattt gtctgctttc cataaaaact
     3301 agcccgcaga cttgctccta ccacaccacc aaaaccggcg agattgttga tcgtgtttgt
     3361 tacccacgca gcaagaagaa actcttttct ttccattttt ggttttcctt gtttttctaa
     3421 gactctgatc gtcacccaat catacaacaa catcggagtg acacccagca gtccagccag
     3481 aatcatgagc aggatcgtca tgcggctttg ttgtcccatc ctttgccaaa tatcttgcca
     3541 actcatgcca ttagcaatat ttgcgacttg atttgccaca aagactaaca cggagccgaa
     3601 aaaaatcagt tttaaaatca gactatgttc ctttatccat tgcagcatca agtatcgttt
     3661 atttttcaca ttttcccatt cctaacttgt atacttttgt gtcatacccc aatgttatct
     3721 tatttatttt aatttttcca ctttagacct gttctttgct tgcgatgata gaaaaagaaa
     3781 gatatcctaa tgctaaagga gaggataaca tgaaagtagg atttatcggc acagggaata
     3841 tggcacaagc tattattgat ggtttattaa aaaaaggaac agtctcgaaa gaagaggtgt
     3901 tcgtttccag tggacatttt gaaaatgcac aaaaatttgc tgctgaaact ggcgtccatg
     3961 catgcaaaag taatcaggaa gttgcatcac aatcagatat cgttctttta gccgtgaaac
     4021 caaaaatcat tgaaaaagta ttggaagaac taagagaata tagccagaaa gtcttattca
     4081 tttctattgc ttcaggaaaa tcattaacag atttagaaac agcgcttgaa gatcctcagg
     4141 cctccatcgt acgtgtgatg ccgaatgtca atgtcagcgt aggagcaggc gtttctgcga
     4201 tttgcaaaaa tgaagctgcc acggaggaac agcttcagca agctacgatg atttttgaag
     4261 caatcggcag cgtctatcct ttagcagaat cagatttcag tacattcatt ggccttgctg
     4321 gcagctcacc cgcattcgtt tacatgctga tcgatgcgat gggacggact ggtgtactga
     4381 atggtttgtc caaacagaca gccactgaga ttgctgcaaa agcaattatg ggttcttgtc
     4441 aaaaattcct ttcaagtgat aaaaatccat gggaacttgt agaccaagtt tcttctcctg
     4501 gcggtacaac tgttgcaggt gtagttgcct tagaagaagc aggattcatc ccagcaatca
     4561 tcaaaggaat caatacgacg atcgagaaag atcttgagat gttgaataaa aaataatgat
     4621 agcaatgttt tttcgtacct tacaaaaaaa gaagagctgt ggcttttgcc gcggctctt
//
LOCUS       DAFEOM010000124         4662 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.131, whole genome shotgun sequence.
ACCESSION   DAFEOM010000124
VERSION     DAFEOM010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.131"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(172..474)
                     /locus_tag="LP524_002740"
     CDS             complement(172..474)
                     /locus_tag="LP524_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235726.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(694..1060)
                     /gene="ssrA"
                     /locus_tag="LP524_002741"
     tmRNA           complement(694..1060)
                     /gene="ssrA"
                     /locus_tag="LP524_002741"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1231..2004)
                     /locus_tag="LP524_002742"
     CDS             complement(1231..2004)
                     /locus_tag="LP524_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL2235727.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2148..2498
                     /locus_tag="LP524_002743"
     CDS             2148..2498
                     /locus_tag="LP524_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBL2235728.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2479..3195
                     /locus_tag="LP524_002744"
     CDS             2479..3195
                     /locus_tag="LP524_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2235729.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3195..4643
                     /locus_tag="LP524_002745"
     CDS             3195..4643
                     /locus_tag="LP524_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL2235730.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
BASE COUNT         1550 a          822 c          900 g         1390 t
ORIGIN      
        1 ttataattgg agtggtagtt aattagtgac ttatttttga ttttatagca ctgatactat
       61 aaaatataga tatcatcata ttacacaatc ttaatactaa cttaaaaaat atccctttta
      121 taagtatggt gataaaatcc gttccgggct acctttttta ggtagcctac tttaatcttt
      181 atacctttct ggatcaacga aagtatactt caaatagtca taacgccgat gatcgctccg
      241 tgcgtctggc acatcagtca cgatatcaaa caaaaaatac acatctttct tcattctggt
      301 ttttgttgcc ggaattttaa agtagttctt attagagtaa tagagattga ttaataagct
      361 atcttcgatt gctaaaaaga aaacttctga atcccacacc ttataaaaat ctttgacaaa
      421 tctattcgaa ggatcaaatt taaaccataa ttgtgttttt ccttccatca acataatgtt
      481 cacctcaaaa agagtatacg aactaatgtt ctttttgtaa agtaggaata tcattgataa
      541 tatggatgga acatatatat taaaaagtgt tctttttgaa tgctattttg aatgccattt
      601 tcattcattt aacgaaaatc tgcgaaaata aaaaatgctc aaacccttat aaaataagga
      661 tttaagcatc tagcgaaatc tgctgaaatc tcacaatgga gacggcggga gtcgaacccg
      721 cgtccaaaca catcgccact taaaaatcta cgttcatagt caactcattt aaggattcac
      781 tttacagctt gccgagtgac aggcattctg tatcgctagt ctgatgttct ctttttcaac
      841 ttacagacgg aaagttgaaa cgtatcccac taaagttagg acccttactc gagcacatgg
      901 gcgatgccga gaggatctac gctaactgtt tttaggcagc taaagcgtaa gagttgtttt
      961 cgtttttagc agttatattt aactgtaacg tttttacgta gacgtaacct acgaaacgca
     1021 attcaagctc gactgtgcct gtcgaatccg taacgtcccc gttagctaac aagggttacg
     1081 tgtatttttc atacaaaatt agtatagcat atattgggat aaattcaata aaaaagcgaa
     1141 tcatatccca ttatttatat aataactgcc cctttcttaa gaaaaaataa gcgattttct
     1201 taagaaaaga ggtagcttca aaaaaagatc ttaaatcaga tcaaaatact tcaatgcgtg
     1261 gacaattccg ccttctgtat ttttctttgt gataaaggta gaaagttctt ttaatccgtc
     1321 ataggcgttg cccatagcga ttttgttgtc gcaagcttcc aacaaggcaa aatcattcgg
     1381 accatcgcca aatccaaaag taggaacttt ttccagctga agttcttttt gcagacgttt
     1441 gacacctgcc ccttttgaga ttcctttctt cactgtatcg atcgagtaag gaccattccg
     1501 ataaaaagtt agctcaggaa aatgttcgtg atagtattcg tcacctgatt gagataagat
     1561 cagcaacata tttactgtgt tttctttaaa gcctaaagga tcaatttccg gaacatctga
     1621 atgaatgaag tcgtaagcat tgatcattgt ttggctatga ccggtacacc agattttctg
     1681 atcgttatag taagaaagtt catgaccgtt caactgtaca tgatcataca ttttttgaca
     1741 ttcttctgaa gaaaatgttt gagaatagat ttcttcgccg tctactcgaa tgaaagaacc
     1801 attcatggtg accgcagaag taatgcccgc atcttccatg atagagtgga tttctgcttc
     1861 tgtacgtcct gtagcgatga cggggagaac attgttttct tttaatgcgt tcatagcttt
     1921 tgccacttcg ggtgtgattt ttgatttcgc atctagtaat gttccatcaa gatcaaagaa
     1981 agtgattgca cgataatttg acacaaataa aactcctttt ttattggaat aagtttatct
     2041 taacaaagaa tgaacaaaag acaaaccttt cgtgaagaat tcctgaacat attggttcag
     2101 tcaacaattt agagtatact ataagaagag aatttaggag ggaaagaatg atttttttca
     2161 gtattggtat cttattctta ataattggca gtctttttcg gattctgccc tcaaaaggaa
     2221 atctgccttt ttatgggtat cattcgcctc ttgccgcaaa aacagacgct cattggcggc
     2281 tggctcaaaa gacaagcgga aactggtttt ttcttatggg cttactgatg gcattgatcg
     2341 gctattattt aaagacatct gggcatacga attattttct gattgaaatg cttctgctag
     2401 tatttccaat tatgcctatt tttattatga cagaaaagaa attacagaaa tatgacctgg
     2461 aaactggagg gaacgataat gaatatttta atgattgaag ataatcaatc tgtttctgaa
     2521 atgatgcaga tgtttttctt aaatgaaggt tgggaagcaa ctttcaagta tgatggaaaa
     2581 gaagggctcg atgccttttt agaaaaccca caacattggg atatgattac actagacctg
     2641 aatttgccca cactggacgg tatgctagta ggaagagaga tccgcaaagt ttcaagtact
     2701 gtgccgatta tcatgctgac agctagagat tctgaaagcg atcaggtgat cggcttagag
     2761 atgggggcag atgattatgt cacaaaacct ttcagtcctc tgacgcttat tgctcgaatg
     2821 aaagctttgc atcgccgtag tgagttggca gaagcaagag aagaagcagt agtttcagat
     2881 gatcattttg atatcgagac tagtcatttc aagatgaata cgaaaacacg agaagcgtat
     2941 ctggacaacc ggccaattga aggactgacg cctaaagagt tcgatctatt gtttacttta
     3001 gcaaaaaaac cacgtcaagt attctctcgg gagcagttgc ttgaattagt ctgggattat
     3061 cagtactttg gtgatgaacg gacagtggac gcccatatca agaaattacg acaaaaaatc
     3121 gaaaaggtcg gaccacaagt catccaaact gtttggggag tcggctacaa attcgatgat
     3181 tctggtgttg cctaatgcgt tatttgtatc agcaactctt agctttctgc ggaatggtcg
     3241 cgttgatctt attgatcgtt ggagtttctt ttacacaact gacaaaacaa acgattgaag
     3301 aaaacaatta ccagcaactg tttggatatg ctgaatcagt tgaaaagaca acgcaaacct
     3361 atgccgatac gctcccgcag tttagtcaag atcaggcgtt tcaaaatgca ttgctgctga
     3421 cagagcaagt actgacagaa caaaatgtga actttatatt tatcgataaa aacgaacgtg
     3481 tcatttatcc gactaataca gctttactga attttacctt aacagctgat caatggaaaa
     3541 atctgaggaa tggacgtcaa gaaaaattca catcgaataa aaatatttta ggacaagcac
     3601 aggcgacctc ttacgcactt gtgccattta atttgaatca tgaattctat ggagcacttg
     3661 tagttagtca acctgcgcga aatatcgata acagcgttcg tccaattaca ctgaacctgt
     3721 tcaaaggatt tattttttcc agtatcgttg cagttattgc cagctatgct tttgcagcat
     3781 tccaagttaa gcgtatcaat cggttaagga atgcgacgaa agaagtgacg aatggtaatt
     3841 ttgatgtaca gctccccgta catgacaaag atgaatttga tgagttagca gatgatttca
     3901 ataaaatgac gaattcattg aaagaatctc aagcagaaat tgaagaacaa gaaaatcgtc
     3961 gaagacaatt tatggcagat gcttctcacg agatgagaac acctcttacg acaatcaacg
     4021 gacttctgga agggttagaa tacaatgcaa tccctgaaaa tcagcgagaa aacgcgatta
     4081 aattgatgaa aaatgaaaca gagcgtctga tccgtttggt gaatgaaaac ttggattatg
     4141 aaaaaatccg tacgaatcaa atcagtatgg tcattaaaaa attcaatgga acagaaacat
     4201 tgaaaaatat tgttgcacag ttagaagcta aagcagaagc agcaggagat acattgacat
     4261 tgaaagccgt tgatgatatt gatgtttacg ctgattatga ccgatttgtc caaatcatgg
     4321 tcaatattat tcaaaacgcg atacagttta ctgaaaacgg ccaaatcacg attactttag
     4381 aaaaaggcta tcttgaaacg atcatcacga ttgaagatac gggaattgga atgtctgaac
     4441 aacaaatgaa aagtatctgg gatcgttatt ataaagtaga tccttcaaga aaaaatacaa
     4501 aatatggtga atcaggatta ggtttaccaa tcgtgcagca acttgtacgc ttgcataaag
     4561 gaaaattaga agtagaaagc gaactaggaa aaggaacaaa attcgctgtt cgtttgcctg
     4621 acgtagaaat caaaaaagag taaaaaacaa gcggtataaa ag
//
LOCUS       DAFEOM010000125         4639 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.132, whole genome shotgun sequence.
ACCESSION   DAFEOM010000125
VERSION     DAFEOM010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.132"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            116..976
                     /locus_tag="LP524_002746"
     CDS             116..976
                     /locus_tag="LP524_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HBL2235731.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQEIKNYQIKNKLICHCSGAISS
                     DIFSNIQDYGAYGYSVHPMFSISDKYNSYKKLKKSFITIEGDEKYAKFLCDFFKKLGN
                     STVIVSKENKSLYHAASVVSSNLVLGLINTSVTYLIQCGFTEKMAIEALYPLIEFNIR
                     NIKEKGIIESLTGPVERCDISTIKGHCEVLSDEDRALYMLLSKNVLEIAMLKNINRDY
                     SELEKYLGES"
     gene            988..1815
                     /gene="panB"
                     /locus_tag="LP524_002747"
     CDS             988..1815
                     /gene="panB"
                     /locus_tag="LP524_002747"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBL2235732.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            1830..2678
                     /locus_tag="LP524_002748"
     CDS             1830..2678
                     /locus_tag="LP524_002748"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBL2235733.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            2688..3083
                     /locus_tag="LP524_002749"
     CDS             2688..3083
                     /locus_tag="LP524_002749"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HBL2235734.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
     gene            3285..4109
                     /locus_tag="LP524_002750"
                     /pseudo
     CDS             3285..4109
                     /locus_tag="LP524_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4032..4328)
                     /locus_tag="LP524_002751"
     CDS             complement(4032..4328)
                     /locus_tag="LP524_002751"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL2235735.1"
                     /translation="MSAVVTDLTYVRVGTNWAYICLMIDLFNREIIGHSCGNKKDAQL
                     VKRAIQSIPYSLQEIELFHTDRGKEFDNQTIQNLMNGLVIKFLSTVCMKKLDVC"
BASE COUNT         1743 a          574 c          923 g         1399 t
ORIGIN      
        1 gatattatga taaattaact atggatgata tttgaaaaaa acatactgta tatttgattt
       61 aatttttatt tttaagctca tatcgtaaga atgggacttg tagtctcact tctttatgga
      121 ggtgataatt atgaacgttg gttttattgg agccggcaag gtaggttgtt cattcgggaa
      181 gtatttccag gaacataaaa tacaagtaac aggattttat agtaaaagcg aagactcgtc
      241 attagcagct tcgaatttta catctagtaa acaatattta aacttaagag aattagtaga
      301 tgaaaatgat actattttca ttacgactcc tgatggtcaa attcaggagg tatggcaaga
      361 aattaaaaat tatcaaatca aaaacaaatt aatctgccat tgcagtggtg caatatcttc
      421 agatatcttt tcaaatatac aggactatgg tgcttatggc tattctgtcc atcctatgtt
      481 ttcaatatca gataaatata attcatacaa aaaattaaaa aaatctttta taacaattga
      541 aggcgatgaa aaatatgcaa agtttttatg tgatttcttc aaaaagttag gtaatagtac
      601 tgtaattgta tctaaggaga ataaatctct ttatcatgcc gcatcagtag tatctagtaa
      661 ccttgtgcta ggacttatta atacaagcgt tacatatcta attcaatgtg gatttactga
      721 aaaaatggca attgaagctt tatatccatt aattgaattt aatattagaa atataaaaga
      781 aaaaggaatt atagaaagtt tgacagggcc agttgaacgt tgtgatattt caacaattaa
      841 aggtcactgt gaagttttat cagatgaaga cagagcttta tatatgctat tatcaaaaaa
      901 tgtattagag atagctatgt taaaaaatat taatagagat tatagtgaat tagaaaagta
      961 tttaggagag agttaggaga gaaatagatg aaaaatacag cagttacatt taaagaatct
     1021 aaattaagaa atgaaaaatt aacaatgctt acagcttatg actattcaac agcaaaaata
     1081 attgatgaag ctgggataaa tggaatatta gtaggagatt ctcttggtat ggtatgtcta
     1141 ggacatgaag atacactttc agtaacaatg gaagacatga ttcatcatac tagagcagtt
     1201 acaagaggag ctaagaacac tttagttgtt gcagatatgc catttatgtc ttatcaaact
     1261 tcagtatatg attcagtagt taatgcagga agattaataa aagaaggacg agctcaggtt
     1321 gttaaattag aaggagggat agaagtatgc gataaaatag aggctatagt aaaggcttct
     1381 atacctgtta tggcgcatat aggtttaaca cctcaatctg ttaatgcatt tggaggattt
     1441 aaggtacaag gaaaggacaa ggaagctgct aaagaattaa taagagctgc taaagctgta
     1501 gaaaaagcag gagcattcgc tgtagtgtta gaatgtgttc caactaagct tgcagagtta
     1561 atttctaaag agatatcaat acctacaatt ggtattggag caggtgcagg gtgtgatgga
     1621 caaattcttg tatatcaaga tatgttgggg atgtatagtg attttacccc taaatttgtg
     1681 aagaaatatg ctaatttatc agaagagatg aataaagcat ttacaaaata tatagaggaa
     1741 gtaaaagatg gagtttttcc aggtccagaa catgggttta aaataagtga tgatgtttta
     1801 gaaaaattat attaaaaggg tcaagaaata tggttatatt taaaacagtg aatgatgtta
     1861 gaagtcaagt aaaagagtgg aaaaaacaag gcttaaaggt aggattagtt cctactatgg
     1921 gatatttaca tgaaggtcat gaaagtttaa ttagaaaagc atcaaaagaa aatgataaag
     1981 ttgttgtaag tatctttgta aatccaactc agtttgggaa aaatgaagat ttaggtagtt
     2041 atccaagaga cttagaaaga gatatagaag tatgcacaag aggtagagca acagcaatat
     2101 ttaatcctga agttgaagag atgtattgtg acaatgcaag tacttttgtt aatataactg
     2161 gattaacaga aggtttatgt ggagctagta ggccaataca ttttagaggg gtttgcactg
     2221 ttgtaagtaa attatttaac ataataccag cagatagagc ttattttgga gaaaaggatg
     2281 cacaacagtt agctgtaatt aaaagaatgg ttagggacct taatattgat atagacgttg
     2341 tagggtgtcc aattataagg gaagaggatg gactagctaa aagttctaga aacacatact
     2401 taagtttaga ggaaagatct tcagctacta ttttaaataa atctttaact ttagctaagg
     2461 aagctttaaa taatggagag agagacagct taaaaataat tgagataata agtaaaaata
     2521 taaatactta taatttggca aagattgatt atgtagaagt tgttgattct ttatcattac
     2581 aaagagtaaa ttatattgaa aaatcagttt tagttgcaat agcagtattt ataggaaaaa
     2641 caagattaat agataatttt acatttgaat tacaataggg ggctataatg aaagttacaa
     2701 tgttaaaagg aaaaatacat agagctacag ttgaacaagc tgagttagat tatgtaggaa
     2761 gtattactgt tgatatgaat ttattagaag cagcaggaat atatgaatat gaaaaagtac
     2821 aaatagttga tgttaataat ggaaatagat ttgaaactta tacaatagct ggagagagag
     2881 gaacagggat gatttgcttg aatggagctg cagctagatg tgtatcaact ggagataaaa
     2941 taattttaat ggcatattgt gaattaaatg aaaatgaggt taaggatcat agacctaagg
     3001 ttgtttttgt cgatgataat aataaagttg aaagagtaac atcatatgaa aaacatggaa
     3061 ggttatcaga tataattctt taggttttgt aatgttattt atatatagct agactattat
     3121 tgaaatattt attgattact aattggatag atgtcaataa agtagacaca aaaagagaag
     3181 atacagactg tagacaaagt ccattttatg tgaatcggct acactagact agacagaaaa
     3241 aataaggtgt gtagaataat aaaaaacaca ctggaggatt tttcatgtca agacgtcaac
     3301 gaagaaccta ttcaaaagaa ttcaaacaac aaatcgtcaa tctctatctc gctggtaagc
     3361 ctcgcgcaga aattattcga gagtatgagc ttacgccttc ttctttcgat aaatggatga
     3421 agcaagcaca atcaacgggc tcattcaaaa aaagagacaa cttaacacca gaacaagcag
     3481 aattgatcgc actaagaaag aaaaataagc aactcgaaat ggagaatgat attttaaagc
     3541 aagcggcgct gatattcgga cgaaaagaca agtaattgat gccaacaagc ataaatattc
     3601 catatcagcg atgtgcaaaa ttctaaatat ttctcgtcag acctactatt atcaagcgaa
     3661 accgaaaggc ttacggtacc cgaaaattga agaagtgttt agcaaagcgt gggcttcaac
     3721 tcagtcggcg ccgaatcggt cgaatcatga aacgccgcgg attgacatct acctatacga
     3781 tcgctcattt taaagggcaa cgaacagctt gtaatgaagc gaaaacagcg aatgtattag
     3841 atcggacctt tacacaagaa cagccattgg aagccatcgt tacggatctt acttatgttc
     3901 gcgtggggaa aaagtggcat tatatctgct taatacttga tttgtttaat cgagaaatta
     3961 ttggttattc ctgtggtgag aagaaagatg cctcattggt aaaagaagcc tttggacgga
     4021 taccgtattc tttaacagac gtcaagcttt ttcatacaga ccgtggaaag gaatttgata
     4081 accaaaccat tcatgagatt ctgaatggtt tggttatcaa attcttttcc acgatccgta
     4141 tggaagagtt cgatttcttg aagtgagtaa ggaatgcttt gaatggccct ttttacaagc
     4201 tgcgcatctt ttttatttcc gcaagaatgg ccaataattt cacgattgaa taaatcaatc
     4261 atcaaacaaa tataagccca atttgtcccg acccgaacat aagttaaatc cgtgacaacc
     4321 gctgacaatg cttttgtttg agtaaacttt cgatctaagt gattcgcaat tttcttttga
     4381 ttgctttggc acgatcataa agacgggagc agatattgtc tattgatata atgatagtag
     4441 acaaacagag ttggctgatt agctaaagca tatcgagtat aatttgaata ttagacgcat
     4501 gatttatcag tgtaagaaag ggtgaggatt atgaaaaggt ttttgaaaat atttatcttt
     4561 ctcgaaataa tactttttgt atatattttt aatacttcta tttacaatat ttatgaaaag
     4621 aataatagag agtgtaaaa
//
LOCUS       DAFEOM010000126         4618 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.133, whole genome shotgun sequence.
ACCESSION   DAFEOM010000126
VERSION     DAFEOM010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.133"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(15..962)
                     /locus_tag="LP524_002752"
     CDS             complement(15..962)
                     /locus_tag="LP524_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBL2235736.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(1140..1943)
                     /locus_tag="LP524_002753"
     CDS             complement(1140..1943)
                     /locus_tag="LP524_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HBL2235737.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            2089..3003
                     /locus_tag="LP524_002754"
     CDS             2089..3003
                     /locus_tag="LP524_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBL2235738.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            3051..3521
                     /locus_tag="LP524_002755"
     CDS             3051..3521
                     /locus_tag="LP524_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBL2235739.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            3606..4214
                     /locus_tag="LP524_002756"
     CDS             3606..4214
                     /locus_tag="LP524_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBL2235740.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            4348..4548
                     /locus_tag="LP524_002757"
     CDS             4348..4548
                     /locus_tag="LP524_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL2235741.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT         1512 a          716 c          912 g         1478 t
ORIGIN      
        1 attgttttca gctattaata tcctgcagac aattttactt gattggtttg aagctcatgg
       61 gtggcaaagt agcttttcag attggttaaa aaaatagcca ttaatttctt ttgaaaatca
      121 ggtgtcaaac ctgcaatgtg ggaagtaatc agtacattgt ccattttcca aagaggatga
      181 tcttgtggaa gaggctcttc ctcaaaaacg tctaatgctg caaaggaaag ttgctggtta
      241 tttaaagcct gcaccagatc ttgtgtctta acagaagcac cacggccaac attgatgaat
      301 acagattttg gatcaaagtg ttcaaacata tgctgatcaa agagcccagt tgtttcctcc
      361 gttagaggta agatattgac tacgaaatct acttcaggaa gaacttcctt tagttgattt
      421 aaagaaaaag tcttttccat tccttctgct ggatgaccgg atgtattgat tccatagcag
      481 ttcatgtcta atgctttgca tttttttgca atcatttttc caatatggcc agttccaatg
      541 attagtattt ttttcccttg gatagattgg taatggaccg atgaacctaa ccatttcatg
      601 tcactctgtg ccttttgtgc ttgaaaaaga ccacgagaat acccaaggat gattcctaag
      661 acatgctccg taatcgattc actgtggatg ccactaccat ttgaaagtaa aatatgtttc
      721 tttgaaaaag tctcaagggg tagctgatca acaccagctg agatagattg gacccatttc
      781 aaggtagaag aggctaatag tttttcttgg tagtctgagt tccagcccaa actgatctct
      841 acattttgta gatcctctgg ttgtaattct gttttaaaga tataaccagg cgctatagta
      901 tggatttctt cgataaattc atcacgaaat ggacgatctg cataaatgat tctttctgac
      961 atagttaatt tcccgccttt ctttactcta agtgtagcgc atacaagaag aactttgaag
     1021 cgccttgctt gttttttgag aaagaaatat ttttcaaata aaaaaggggc tgtgacaaaa
     1081 aaatgtcaca acctctaaaa gggtctattg aagtagaaat aacgagttgt ctttatttct
     1141 taattgatcg atatttttgc ccatcctttg acagcttgag caaaaataaa gattcctgca
     1201 aaagcaatga aggcgctagt gtcaaaagaa ggaagaaaga ccatcaagat tgttgcaatt
     1261 atagtaggca atgaaattga atagatcaat gtttttaaac aatcaagaaa agtagctttt
     1321 gttagtcgaa agcgtgcata gagaaaagca gcaatgttgg taaatagcaa agtgatcagc
     1381 agattcagaa aactaggata aatagaaaat ataaatgtca ggacttttat ccaaagtggt
     1441 atagatgctt cacttaaaaa agaacggagc tgcttacctg ttaagttttt taaagattca
     1501 ttggtgtagg gaagttctaa ttggttattg ttcaacaaag cagaagtcgt gcctgtatta
     1561 ggaagagcta tgataagttt gttttttaac atcccgacac taacaaaatt ccccatcaaa
     1621 tcagtagaga tgtcctgttc tgttcgtttt ccctctggat caaatgtgaa gataatggag
     1681 tttgtttggt aaatgaatcc ttctttttct tcggtttgaa gctgcccgtt ttggattgta
     1741 aaatcaggaa tgcgagtagc aatcttttgc ccatccaatt gaatgttgtt taagacttga
     1801 aaaacttgaa aagagattgg caaagccata attatactca aaactaaaag atagacaatg
     1861 gctttccaaa atgggacatt tctcgcctcc tttaggtctt tgaatcgtat aaaggaactg
     1921 ataattaatt gttttgttgt cataagtact cctcatctag tagtgtcatc acttgcaatt
     1981 ctagcgaagt atcaagtgaa atacaagtgc tttaaggaaa cagctgaatg attggtgaaa
     2041 tatcaaaaaa atgttattct tgtctaaaga ctatccaata taaataaaat ggaggaagga
     2101 atagtgccta cacagccaca gagatcatca aaaaaactat ttggagtagt ggttttgaat
     2161 gcggtgatca ctttatcaga atttattgga gggattatgt cagggagttt gtctcttgtt
     2221 tcagatgctt tccataacct tagtgataca ttggcaatta ttttttctta cggggcacaa
     2281 aaaatggccc gaaaagaagc aaataaaaaa cgtacgtatg gctaccagcg tttagaaata
     2341 ttgagtgctt tcatcaactc attcatattg atcatattat ccttgttttt aaccgttgaa
     2401 gcattcaagc ggttcacttc accagaaaag ataaatagtc atttgatgct aacagtagca
     2461 gttattggat tgctcgcgaa tttgttctca gtattattat tacgacaaga agcagatgaa
     2521 agtttgaata taaaatctag ttatttgcat ttgttaagtg atacactgtc ttcggtaagt
     2581 gtgattatcg gagcaatttt gatccgtttt ttcgatatct actggatcga tccagttatt
     2641 acactgatca tttcgatata tatattgata gaagcgataa tggtgatcaa aaaagctgcc
     2701 gctattttga tacaaagtgc acctgctatc gactatgaaa aaatggaaca agaaattgaa
     2761 gcaatcgatg gggtaaagga tgttcatcat gtccatattt ggcagtatag cgaaaagata
     2821 attattttcg acggacatat tgattttgaa gatcagctac taagcgagat tgaaaagata
     2881 tatcctcgta taatttcttt attaaaatta aaatatggta ttacacatgt gacgatccaa
     2941 gcagagacaa aggcaaagga tcaaaaaaaa ttaatttttt taaataaaga tgaaagttat
     3001 tgaaatatag agtatgataa ataagaattt ttaataaggt aggttggctc atgaagcagt
     3061 atcgacagtt aaaaggatat ttagaagcaa aaggatattg ggagatacta gtctatctct
     3121 tttttggtgg tttggctacg attgtaaatt ttgttagttt tgctctcgca cgccaatatt
     3181 ttgatttaag tatggcctta tccaattcta tttcgtggtt ttgttctgta ttgtttgctt
     3241 ttgtaacgaa caaactttgg gtattccatt caaaatcacc gaattttaca catgcactta
     3301 ttgaatgcgg taagttcttc ttctaccgta tcctttctta cggattggac atgggggcaa
     3361 tggtattgtt gatcaatgtt atgaacagca atgagtatgt ggcgaaaatc atcactcaaa
     3421 ttattgttat ccttgcgaac tatattttca gcaagttgtt tatttttaaa gaaactgagg
     3481 tttttgaaga agaagtaggt caaagtgaaa aagacgacta ggcataacaa ttgaagaatt
     3541 tccttctctt tgttactctt tgtaatgtag acattattaa gaaaacattg aggaggaatt
     3601 tttttatgac ttacacatta ccagatctac cttatgcata tgacgcattg gaaccttata
     3661 ttgacgaaga aacgatgcat ctgcatcatg ataagcatca caatacttat gtgacgaatt
     3721 taaattcagc aattgagaaa tacccagaat taggcgaaaa aacaatagaa gaattattat
     3781 ctgatatgga cgctattcca acagatatca agacagctgt acgtaacaat ggtggcggac
     3841 atgctaacca ttcatttttc tgggaaatca tggcaccaaa tgctggtggc gaacctacag
     3901 gagaaataaa agaagcgatt aatgaagctt ttggtgattt ttcttctttt aaagaagaat
     3961 tcaaaaaagc agccgctgga cgatttggtt ctggatgggc ttggcttgta atggaaaatg
     4021 gaaaattagc tattacctct actgcaaatc aagattctcc attgatggaa ggaaagacac
     4081 caattctagg tttggacgtt tgggaacatg cttattattt gaaatataaa aatgtccgac
     4141 cagattatat tgcagcattc tggaatgtta ttaattggga tgaagtaaac aaacgtttcg
     4201 aagcagcaaa ataaccattt cctccgtcat atcagtctta aaaataattt ggaaaataaa
     4261 agagcgaaag cttgctagcg ttttctaaat tgggtataat gttcttgcaa ggttacttgc
     4321 aagaactttt ttggaggata tacatttatg gaacacggaa cggttaaatg gttcaacaat
     4381 gaaaaaggat ttgggtttat tacagtagac ggcggagatg acgtatttgt tcatttctca
     4441 gcaattcaag gagacggctt caagagccta gaagaaggtc aagaagtaga attcacgatt
     4501 gttgaaggtg cacgcggacc tcaagctgct gaagtgacta aattataata atttgttgaa
     4561 taaagacacg acttcttaga gagtcgtgtc tttttgtcta tttatgaagt ttttagat
//
LOCUS       DAFEOM010000127         4480 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.134, whole genome shotgun sequence.
ACCESSION   DAFEOM010000127
VERSION     DAFEOM010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.134"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(590..2095)
                     /locus_tag="LP524_002758"
     CDS             complement(590..2095)
                     /locus_tag="LP524_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL2235742.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2092..2778)
                     /locus_tag="LP524_002759"
     CDS             complement(2092..2778)
                     /locus_tag="LP524_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL2235743.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2971..4392)
                     /gene="gndA"
                     /locus_tag="LP524_002760"
     CDS             complement(2971..4392)
                     /gene="gndA"
                     /locus_tag="LP524_002760"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBL2235744.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1249 a          928 c          736 g         1567 t
ORIGIN      
        1 cctacacatt tggtttgtct tactttgttc agttttcaaa ggtctaatct ttttgtttta
       61 acaactcttt aatcttatca gattcagaag agaatgtcaa caacttcttt gtttattttt
      121 tctaaacgtc tttgccactc attagcagcg acttgattat cataccatct ttattgttat
      181 ttgtcaataa ctttttcgaa gtttttttcg ataaaatcaa gctttattag ctatctcatc
      241 caaacgactt cgttattata acatgacatc aaaagtgcgt caacactatt ttttataatt
      301 tttagtgaca ccttaaagga atcatttaga acaaataata atatagcaat ctctttataa
      361 aattgcaata gaaaattccc tatttttatt tattattttt aaacacgaat attacagcat
      421 tttattgttt tttcgttcat ctaaaagata cttacattta attaacggat gttattctat
      481 ttaattgttc gctagttttt ttataatgac atccctgaat ttctcacttg cttgtatttt
      541 gtaccttatt ctttgttgga tatataaaaa aacaagtaaa gcacatgctt tacttgtctt
      601 ctgcctttgc gataggaata aagattctaa aaattgtccc ttgccctact acactttctg
      661 ctaagatacg tcctttatag ttttcaacca gttgtttagc gatagacaac cccagaccat
      721 ttccaccctt cgttctagct cttgctttat ccactcgata aaaacgattg aagatcttat
      781 taaggtcttc tttgggtatt ccttcgccaa agtcctgaat cgcgatctca aattcattca
      841 gagtggaaga aatagagata tggacttctt ttcgtgtagt tgaatacttt actgcgttat
      901 ccaatatgat aatgattaat tgttcaaagt gattacgata aatttgcagg gtcacttctt
      961 ttgtcaaatc atcatctaaa gtgatcacat agtctggata taatatcttg aaattattat
     1021 aaacttgata ggtgacttct tttcctttag ttgtttcatt cgcataatga acatctactt
     1081 gttctgctct tgataagtct agcatctctt gaactaagct tttcatacgg ctgatttctt
     1141 gcatgctggc gcttaatgat tcttctaata tctcaggatc atctttcccc caacgattta
     1201 gcagactaag atgcccttca ataattgcaa caggtgtcct taactcatga gatacgtctt
     1261 ccacgaattg ctcttgttgt tcaatataca aacgcatacg gtcaagcatc tcattgaata
     1321 tttcagctaa atctgccaat tcatcatttg tgtcgatttc aggcatgtgg atatctgatt
     1381 gcggatcttt acgtatagta tccattgtat ctctgagtac cttgagtggt tttaagaaat
     1441 aagaagataa aaagaaacct aaaatactac ttaagatcaa agaaacaact tctaaaacaa
     1501 taagagtcaa taatagctta tttcgaatat catagaaaga agataattca tagaagcttt
     1561 gcgcatagcc gatcttttct cttgtacctt tagaatatat aggctggata gataaaaagc
     1621 cagtttttcc ttctagtgta acgatcgttg gaactttacg tgtagtctga atcaggtttc
     1681 gactgttttc atgggtttta aaaatcaagt tctcgtccaa atcataaaca taaagatcca
     1741 ttgatggctg ccctagctca gctataaagg aatcgatctt cattaagttt ccttcgacca
     1801 gtgaagtatg atctacgacc tcatctccgt catttgtcgc tttcgtcaga ttacgatacg
     1861 tatttactaa tgtcagttct tcatccgagt tagccaaacg agaagctact tctgaaatcg
     1921 tacgctctac attgtttctt tcttttgcca caatcagatt gatagatgat ttataggtaa
     1981 tgacagcgaa aattgtaaac acaacgaata tgaagaaaga actcgcaaag gcccacttga
     2041 ttgtcaagga gggccctttc agttctcttt tttccgattt tttcggcttt atcacgagcg
     2101 catcacataa cctgttccgc gtacagtttg gatatagctt tcctccccgg gaacgtcaat
     2161 tttgttgcga agataacgaa tatatacgtc cactacgttt gtttcaactt ctgtttcgta
     2221 tccccacact ttatttagta aaacgtcacg tgctaatacc acgttcacgt tttccattaa
     2281 agtaagcagc aactcgtatt ctctttttgt tagttcaatt acttcatttc cgcgacgtac
     2341 gacacggttt tctttttcga tagtcaaatc gcgataagta atagtagttt gtttagccac
     2401 atttttatcg ccttcaatat cgattcgacg caacaatgca cgaagacgtg ctaataattc
     2461 ttcgatagca aatggtttga cgatgtagtc atctgctccg tggtcaagtc cagatacacg
     2521 atcgatcaca gaatctcttg cagtcatcat aatgattggt gtatttttga cttggcgtac
     2581 acgtctgcag acttctaaac cgttcaattc tgggagcatc agatccaaca atatggcatc
     2641 ccactcatta tttaatgccg catctaaacc agttcggccg ttgtagtgga cttctgcgtt
     2701 atacccttca tgcttcaact ctagctcgac aaatcgtgcc aagttctttt catcttcaat
     2761 gattagaata ttgctcattt attattttat cctttctatt ctactggctt tttaaaaact
     2821 ctatcattat accgataata tgcttaaaaa tctatagtca ttttatcatg gatagcaatt
     2881 ttaaaaaaga gctgaagtct actcataaca atcgacttca gctctttcgt cctcttttta
     2941 ttttctcatt tcctaacagc cgaaagacta ttattcttca tggtaccatg agtaatggaa
     3001 gattccttct ttgtcgacac gctcataggt atgtgcgcca aagtaatcgc gttgggcttg
     3061 gattatattt gcaggcagac gttctgctct gtatgaatcg aagtaagcga tggctgatga
     3121 gaaagtcggt acaggtacgc cagcttggac agccaaggca acgacgtctc tgactgattg
     3181 ctgatatttt ttcgtgattt caacgaagta atcatctagc aataaatttt cgatctcagg
     3241 atttttgtca taggcatccg taattttttg caagaatcga gctcggatga tacatccagc
     3301 gcgccagatt tttgcgattt caccaaatgg caagtcccaa tcaaattctt ttgaagcgac
     3361 acgtaattga gcaaatcctt gggcataact catgatttta ctgaaataca acgcttctcg
     3421 gattttttcg accaattctt ttttgtctcc cgtgaactca aaatcgttcg tacgagacaa
     3481 aatcttgctt gcttgtacgc gttcttcttt ataagcagaa atatagcggg caaatacaga
     3541 ttcagtgatc aatggaagtg gcacacctaa gtcaagcgcg ctttggcttg tccatttacc
     3601 tgttccttta ttcccagctg catcaaggat cacatctact actggtttac ctgttccttc
     3661 atcgtcttta cgtgtcagaa tgtcagcagt gatttctatc aaatagctgt ctaattcgcc
     3721 ttgattccat tctttaaaga tatcagccat ttcttctact gaaaggttta aaatattctt
     3781 cattaagtca taagattcag caatcagttg catatctcca tactcaatac cgttgtgtac
     3841 cattttcaca taatgtccgg ctccatttgg gccaatatat gtgacacatg gtgctccgtc
     3901 ttctgctttt gcagaaattt gctctaagat tggtgcaact agcgcatagg cttctttttg
     3961 tccaccaggc atgattgaag gtccttttaa agctccttct tcaccaccgg aaacgcctgt
     4021 accgataaag ttgatgccag agttggctaa ctcttcatta cgacgcatcg tatctttaaa
     4081 gaatgtattt cctccgtcaa tcaaaatatc gcctttatca aggtgaggaa gtaactcttg
     4141 gattgttgca tctgttgccg gtcctgcttt gaccattagc aagatccgac gtggtttctc
     4201 gatagattct acaaattctt cgattgtata tgttgctttg aaatttttat ctgggtgttc
     4261 ttctactaca gcggttgttt tagcccctgt acgattgaat aaagccactg aatagccgcg
     4321 gctttctatg ttcaaggcta gatttttacc cataacggcc ataccgacaa cgccgatttg
     4381 ttgttttgac atatgaactc ctcctacata cgggatttct ccctaatctc ttacattata
     4441 gcttaacacg gctacattgc aaactaaaga acaacatatt
//
LOCUS       DAFEOM010000128         4306 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.135, whole genome shotgun sequence.
ACCESSION   DAFEOM010000128
VERSION     DAFEOM010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.135"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            149..1138
                     /locus_tag="LP524_002761"
     CDS             149..1138
                     /locus_tag="LP524_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HBL2235745.1"
                     /translation="MKKKKQDLINGTFREKKKNLKKVVAFSDNRISPRMRELMTGCGS
                     YLEFIATIDKEKKKLVQAHFCKNRFCPLCAWRKARKDSMMLSIMMQAIAEEKQYQFIF
                     MTLTTPNVKAERLNDEIDLFNHALSKMFRRKKVQIAIKGYVRKLEITYNKEREDYNPH
                     FHLILAVNKSYFTDKDYYIKQSEWLDLWREVTGKTGVNPDGTDEITQLDVRKVKGFQQ
                     EKAVQEVAKYSAKDFELTESQEVFDTFYHAMKGRQLITFNGVFKEYKKKYESGDLDRY
                     KKKDENEYFWFLTASWMRKESKYAVDYREMSADERNYFSGMYVDEKSVEGENE"
     gene            1135..1554
                     /locus_tag="LP524_002762"
     CDS             1135..1554
                     /locus_tag="LP524_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235746.1"
                     /translation="MTDSTDKMTIKALADELGITKNTVRNYLVKAGYDLARYKKNGII
                     LLDKELIGVARSIYRSKGGQLTGHLPVNSTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            1533..1871
                     /locus_tag="LP524_002763"
     CDS             1533..1871
                     /locus_tag="LP524_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235747.1"
                     /translation="MVAILEVRCDMDKIDLREETVNRIEVMYERLADYLNLSDEDNYS
                     EMLERMMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDEAEKEFWLKLMSHIMQLNQI
                     SVINSKEKGM"
     gene            1875..2237
                     /locus_tag="LP524_002764"
     CDS             1875..2237
                     /locus_tag="LP524_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235748.1"
                     /translation="MGMTLADIKVQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFESFADFERWLSSISDDQQHEKSVGNELRADEKSAPSERNYPNESDGIFGGIPNRK
                     SQMSESGFASMFSDVNSR"
     gene            2459..3865
                     /locus_tag="LP524_002765"
     CDS             2459..3865
                     /locus_tag="LP524_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HBL2235749.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQINIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKMEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPSLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYICRQREE
                     NEREKALVASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVAALEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSEDEIEW"
BASE COUNT         1501 a          602 c          996 g         1207 t
ORIGIN      
        1 aaaaaaaaag attgcttttt cttccctagt gatttatagt tttaattgtc taagtcaaaa
       61 ctaaactaga aaggagagca atcttaattt ttgttatcgg cttttcagcc ttttttgtat
      121 acaaaatttg tagattcagt atagcaagat gaaaaagaaa aagcaagatt tgattaacgg
      181 aacatttcga gaaaaaaaga aaaatttgaa aaaagtggtg gcatttagtg ataatcgaat
      241 ttctcccaga atgcgtgaat tgatgacagg ctgtggttca tatttagaat ttattgcaac
      301 gattgataag gagaaaaaga aattggtcca agctcacttt tgtaaaaatc gtttctgtcc
      361 gttatgtgct tggcgtaaag ctcgtaagga ttcgatgatg ttgtcgataa tgatgcaagc
      421 cattgcggaa gaaaagcagt atcaatttat ttttatgacg ttgacgacac caaatgtgaa
      481 agcggaacgt ttgaacgatg aaattgattt atttaatcac gcattatcga aaatgtttcg
      541 tcgaaaaaaa gtgcaaatag cgattaaagg ctatgtgcga aaattggaaa tcacatataa
      601 caaagaacga gaagactata atccgcattt tcacttgatt ttggcagtaa ataaatcata
      661 tttcacggat aaggattatt atatcaagca atcggaatgg ttagatttgt ggcgggaagt
      721 gactgggaaa acaggggtca atcctgatgg tacagacgaa attacgcagt tagatgtccg
      781 taaggtcaaa ggattccaac aagaaaaagc agtacaggaa gttgcgaaat attcggcaaa
      841 agattttgaa ctgaccgaaa gtcaagaagt gtttgacaca ttctatcatg caatgaaagg
      901 tcgtcagttg attacgttta acggtgtatt caaagaatac aagaaaaaat atgagtcggg
      961 tgaccttgat cgttataaga aaaaagatga gaatgaatat ttttggtttt tgactgcttc
     1021 gtggatgagg aaagaatcga aatatgcggt tgattatcgt gagatgagtg ctgatgaaag
     1081 aaattatttt tcaggaatgt atgtcgatga aaaatctgtt gagggggaaa atgaatgact
     1141 gattcaacgg ataaaatgac aattaaggcg cttgctgatg aactaggaat aaccaaaaat
     1201 acagtgagaa attatctagt caaagccgga tatgatcttg ctcggtacaa gaaaaatgga
     1261 attatcttgc tagacaaaga gttgattggt gttgctcggt caatttatcg gtcaaaaggt
     1321 ggtcaattga ctggtcattt accggtcaat agcacgggca gtgattttcc tttggaaaaa
     1381 gagaatgagt ttttgcgtga tcaattagct gagaaggaca aacatatttc tgaattaaca
     1441 gaaatgattc gtgatcaaca aaaattgacc ctgcttcagc aacaaaaaaa cgatcagttg
     1501 ttactagaaa atcaagaatt aaaaaatcga aaatggtggc aattctggaa gtgagatgtg
     1561 acatggataa aattgatttg agagaagaaa cagtcaatag aattgaagtg atgtatgaac
     1621 ggttagctga ttatttgaat ctttcggatg aagataatta tagtgagatg cttgaacgaa
     1681 tgatggatgt tctcaactcg gttatcaatg ctgatctagc aaaatcttca gttttgacta
     1741 atgaatttgt tcataagcat ttagcgcaaa caactgatga agctgaaaaa gaattttggt
     1801 tgaaattaat gagtcatatt atgcagttaa accaaatttc cgttattaat tccaaagaaa
     1861 aggggatgta gtttatggga atgactttag cagatattaa agtgcaagaa gaaaaattaa
     1921 aaaaagaaca ggcgaaactt cgccaacaga agaaacgatt gattgagaaa gaaaatgctg
     1981 caatgggcaa ggctcttcgc aagcgttttg ggtttgaatc ttttgctgat tttgagcggt
     2041 ggctatcgtc gatttctgac gatcagcaac acgaaaaatc agtgggtaat gagttgcgag
     2101 ctgatgaaaa atcagcacca agcgaacgga attaccctaa cgaatctgac ggaatattcg
     2161 gagggatacc gaatagaaaa agtcagatga gtgagagtgg atttgcgtca atgtttagtg
     2221 acgtaaattc acgataagcg aatattttgc atgaagcaaa ataaagcgta aaaggagttg
     2281 agagcgtgac acgctttcgg ctcctttttt cgttgtcact tgtgacggcg aaaaccctcg
     2341 gagagcgttt ggaattgtga cagtcatgtc acaattagga aaacgttact ctttgacaaa
     2401 gagtaacaga aaggtttaca ctctttttat tagcgacagc gcgaaggagg ggtttaactt
     2461 ggggtttagt atttcgttca aaaagggaac aaagaataca agcatttctc ataataatcg
     2521 tgagttaact gatagccaaa aaatcaatga ctggcataag catattgatt tcacaaaatc
     2581 ggatgaaaat atctatttgg aacaaataaa tattcgtgaa aaatatgaag aattattcgg
     2641 tgaagccgta gaaaaatata atgcaaagca gaaaagagcc gatagaaaaa ttgaggacta
     2701 tttagcaaag gttcgcaagg ataaaaaaat ggaaccacag cgagaattta tcgtgcaaat
     2761 tggtactctt gatgattttc gtactactcg tgatgatggt tcttctactg gcatatcgga
     2821 acaacaagca gaacaaaatc gagtgattgc caacaaaatt ttagttgagt atttcaaaga
     2881 atttaaagaa cggaatccta gtttatccgt ctacaatgct gttattcata atgacgaagt
     2941 aagtccgcac ctgcatttga atattgttcc tgttgctgaa gggtataaac ggggtgttca
     3001 aaagcaacca agttttaaca aagccttgca acagcagggt ttacgagcag ataaagagaa
     3061 tggtcgagtg atttggaata attttaggaa tcaggaagtt gagtcaattg aacgtttgat
     3121 ggcagatttt ggttgggagc gtgagttagt cggtactaac aaaatcaaag atattcatga
     3181 atacaaagaa atcatgagcg aaatttccga attgcgtcaa acggccgtaa aagagcgtga
     3241 gagcgtttct gatgagttga ggtatatttg taggcaaaga gaagaaaacg agcgtgagaa
     3301 ggctctggtg gcttctgaga gagcgaagat tgaagaagag aagaaaaata tgctgaatgt
     3361 aaaaaaagag atggcggtca ttttccaaaa agatatggtt cctttagaaa gtttcaagag
     3421 gcttcctgat ggttcgtatc atttatctgg gaatgatttt gttgatttgt atcaacgagc
     3481 tacgagagca acaaatgcca agagaaatgc tgataacatg agtgagcaat atttacagat
     3541 gcttgagcaa aatacagaat taaatgagta tgtagctgca ttggaagaag agcgtcattt
     3601 gtctggtaga aaacaattag cagatttaca agtggaaaat aaaaagctga aagaagaaaa
     3661 tcgctcttta cgccagcgtt tagagcgatt aaaaaacgca tttcaaaaat cgatcactcg
     3721 tttgtctatc cgttttgggc ttgcagaaaa ggatatgggg ctttctgagg aattagaagt
     3781 attagagcag gagaaaacct tattttatcg tcaaaacgtc tcacagcata aattagagcg
     3841 ttctgaagat gagatcgagt ggtagttacg atgtgcagtc aacggtaccg gaggcggttt
     3901 gtgccgagga taaggagttg acggctaaac cactagccta ttaagctgac atttgctaaa
     3961 aaatggcaag tccatttttt ggccaactta gcgttagcga agtttggggg cgcccttttt
     4021 aaagggtgcg gttttatgtt tgcatgacta agaatgagtg ggaaaattta acagccctat
     4081 ctaacaggcg gttaaatgta caaaatatgt acaccccctt aaaaatgcag tggtcgattg
     4141 atcgaatttt atgtctttgg gttttggggt tttaaaacga aaaaaagggg gcgattttcg
     4201 ccctcttttt tgatgttttt tcttatcttg atactatagg caactatttt cggcagaaaa
     4261 tcggggctat agtatgggtc tatcccttgt ggctctaagg aagaac
//
LOCUS       DAFEOM010000129         4303 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.136, whole genome shotgun sequence.
ACCESSION   DAFEOM010000129
VERSION     DAFEOM010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.136"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            155..316
                     /locus_tag="LP524_002766"
     CDS             155..316
                     /locus_tag="LP524_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBL2235750.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            335..1612
                     /locus_tag="LP524_002767"
     CDS             335..1612
                     /locus_tag="LP524_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2235751.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1622..2278
                     /locus_tag="LP524_002768"
     CDS             1622..2278
                     /locus_tag="LP524_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2235752.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2278..3654
                     /locus_tag="LP524_002769"
     CDS             2278..3654
                     /locus_tag="LP524_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2235753.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(3940..>4303)
                     /locus_tag="LP524_002770"
     CDS             complement(3940..>4303)
                     /locus_tag="LP524_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HBL2235754.1"
                     /translation="GLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDK
                     KLFQARVTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNINVYFTKARFKRLIAI
                     TVSSIVMFLWLFRMGVIP"
BASE COUNT         1681 a          497 c          759 g         1366 t
ORIGIN      
        1 ctgcgcgagg cttaccagcg agatagagat cgacgatttg ttgtttgaat tcttttgaat
       61 aggttcttcg ttgacgtctt ggcatgaaaa atcctccagt gtgtttttta ttattctaca
      121 caccttattt tttctgtcta gtctagtgta gccgattcag gataaggata agtgtattaa
      181 ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat tcagttgaga attgcaatca
      241 tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt ccagtggatg caattagtta
      301 tggagtaaga aaatagagag gagtagttag gaaagtgata aaaaatgcta tagcatatat
      361 tacaagaaag aaaaatagaa ctttaataat ttttatcata ttaactatag ttctttcttg
      421 cttgtattct tgtttaacaa taatgaaatc aagcaatgaa atagaaaagg ctttatatga
      481 aagttctaat tcttcaatat caataacaaa aaaagatggt aaatatttta atgttaatca
      541 atttaaagat attgaaaaat taaaagaaat tgaagaaaaa ataattcaat atgatggatt
      601 agcaaaatta aaagatgcta aagtagttag cggagaacaa agaataaata gagaagattt
      661 atctgacgaa tttaagaatg ttgtttcact cgaagctaca aataatacta aaagaaatat
      721 tttatttagt agtggagtat ttacaattaa agaaggtaaa aatatagaag aaaatgataa
      781 gaattcaatt attgttcatg aagaatttgc taaacaaaac aatctaaaat taggtgatga
      841 agttgatctt gaattactag atattgaaaa aagtggaaaa ataaaaagtc ataaatttaa
      901 aattataggg atcttttctg gtaaaaaaca ggaaacatat acaggattat cttctgattt
      961 tagtgaaaat atggtgtttg tagattattc aacaagccaa gagatattaa ataattcaga
     1021 aaataatgaa attgcaaata aaattttaat gtattctggt agtgcagaat ctacagactt
     1081 agccttaaac aaattgaaag agcttaaaat tgatgagtca aagtattttg ttgaaaaaga
     1141 ttctaatgcg tttgaagagt ctttagagtc agtgagtgga ataaaatata taataaaagt
     1201 aatgacttat tctattatgt taggtgggat ggttgttctt tcattaatct tgattctatg
     1261 gttaagggaa agaatttatg aaataggtat attcttatct attggaacat ctaagataca
     1321 aattataatg caatttatat tcgagttaat attcatatcg ataccaagta taatatcttc
     1381 cttattttta ggaaatgtat tactaaaagt aattgtagat ggattcatta actcagagga
     1441 ttcaatgatt tccggtggaa gtttaataaa taatagtagt tttatgtcaa atataacaac
     1501 acttggacaa agttatttaa tattaataag tattattgtt ttatcagttg tatttgcttc
     1561 ttcattaatg ttaatcaaga agcctaaaga aatcttatca aaaataagtt aggagaaaat
     1621 aatggatata ttagaaataa agaatgtagc atatagttat gcaaattcta aagaaaaagt
     1681 tttgtcagga gtaaatcaaa aatttgaact tgggaagttt tatgcgatag taggaaagtc
     1741 aggaacagga aaatctacac ttctttcctt acttgctgga cttgataaac ctcaaacagg
     1801 aaaaatattg tttaagaatg aagatataca aaataaagga tatagtaatc atagaaaaaa
     1861 taatatatct ttagtatttc aaaactataa tttaatagat tatttatcac caattgaaaa
     1921 tattagatta gtaaataaat cagcagatga aagtatcttg tttgaactag gtttagataa
     1981 aaaacaaata aaaagaaatg ttatgaaatt atctggtgga cagcaacaaa gagtagctat
     2041 tgctagagca ttggtatcag atgctccaat aatactagct gatgagccta ctggtaatct
     2101 agacagtgtt actgctggag aaataattaa tatattaaag acattagcta aagatagaaa
     2161 taaatgtgta atagttgtaa cacatagtaa ggaagtagca gattctgcgg atatcatttt
     2221 agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg aatttggagg ttgaataatg
     2281 ataaaaaatg catttgctta tgtaactaga aaaagtttaa aatcattaat tatcatatta
     2341 gttattttat ctatgtcaac tttaagtctc attagtttat ctattaaaga tgctacggac
     2401 agagcttcaa aagaaacatt tgctaatata acaaatagtt tttctatgga gataaataga
     2461 caagtaaatc caggaacacc tagaggtggg ggaaatgtaa aaggtgaaga tattaaaaag
     2521 atatctcaaa cagatagtat agactcctat gtaaaaagaa taaacagtgt tgcagattta
     2581 gttgattatg atattattga aactcaagag actcttgcga atcaatcacc tgaaagagca
     2641 aagaatttta aaagaacagt tatgttaact ggggttaacg actcaacaaa agaaacaaaa
     2701 tttgtatcag aagcatataa attagtagaa ggaaagcatt tagaaaatga agataaaaat
     2761 aaaatcttaa tgcataaaga tttagctgaa aaaaataacc ttaaagttgg agataagata
     2821 aaaataaaat ctaatttatt tgatgctgat aatgagaaag gtgcagatga aacagtagaa
     2881 gtagaaatta aaggtctatt cgatgggcat aatagcggtg tagtaagtgc agcacaagaa
     2941 ctatacgaaa atacattaat cactgatatt catacagctg ctaaagttta tggtaatacc
     3001 gaagatacag ctgtatacca agatgcaaca ttctttgtaa aaggtgataa gaatcttgat
     3061 agtgtaataa aagatcttgg gaaattagat ataaattgga gagaatataa tttgattaaa
     3121 agttcatcaa attaccctgc attacaacaa tctatatcag gtatttactc aatttcaaat
     3181 aaattatttt ttggctcatt aatatttgca ggagttgtag tttcattatt attattctta
     3241 tggatgaatg ctagaaagaa agaaatagca gtattactat cattaggtat atcaaaatta
     3301 gaaatttttg gtcaattcct aattgagatg gtatttatat caatccccgc attcgttggt
     3361 tcctatttct tagctcaata tacagctgac aagcttggga ataatatatt gaataaggtt
     3421 actggtgata tagctaaaca aatagctaaa caatctgcat ctagccaatt aggtggtggg
     3481 gcagaagctg aaggatttaa caagacatta tcaagtttag atatcaatgt attacctaaa
     3541 ttcatgattt atgtagttat atttatgagt ttagtacttc ttgtatcatt gattatttct
     3601 tcattcaata tattaagaag gaatccaaaa gaattattaa ttgacaataa ttaaaatttt
     3661 cggtgctttt tagcaccaaa aatgagagtg taatataaac cgtgtaagta accatcaatc
     3721 ctaggcgggt ggttgcttca cggtttttat ttttctaaaa taagatcaag aggtgattac
     3781 acgatggcta gaaagaaaag aaatcctgat gctgagaagt tagctgaatc cattttgaat
     3841 acttatcaac ccgaatctgt agaggatatg caagatgttt taaaagatgt gtttggtccc
     3901 ttatttgaaa aaatgcttca aggagaattg aataaccatt tagggtatga cgcccattct
     3961 aaagagccaa agaaacatga caatcgacga aacggttatg gcaataagac gcttaaaacg
     4021 agctttggta aagtaaacat tgatgttccc agagaccggg aggcttcttt tgaacctgag
     4081 ttgattccta atagaaaacg agatgtctcc gacatcgcag actgtagaca aaagctgttc
     4141 caaactcgta actcgagctt ggaacagctt tttatctact tcgtttcgaa tttttgaaca
     4201 aaaaaataaa ggctccttgc ctttccatag ccaggtggct aatttcttta aattcattgc
     4261 agcaaacact agcatcgtat gcgtcgcgac tttttccaag cct
//
LOCUS       DAFEOM010000130         4246 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.137, whole genome shotgun sequence.
ACCESSION   DAFEOM010000130
VERSION     DAFEOM010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.137"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(75..1073)
                     /locus_tag="LP524_002771"
     CDS             complement(75..1073)
                     /locus_tag="LP524_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HBL2235755.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
     gene            complement(1148..1444)
                     /locus_tag="LP524_002772"
     CDS             complement(1148..1444)
                     /locus_tag="LP524_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBL2235756.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            complement(1437..1730)
                     /locus_tag="LP524_002773"
     CDS             complement(1437..1730)
                     /locus_tag="LP524_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235757.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(1876..2427)
                     /locus_tag="LP524_002774"
     CDS             complement(1876..2427)
                     /locus_tag="LP524_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HBL2235758.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2601..2900)
                     /locus_tag="LP524_002775"
     CDS             complement(2601..2900)
                     /locus_tag="LP524_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HBL2235759.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEIGTLPKDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            complement(2927..3235)
                     /locus_tag="LP524_002776"
     CDS             complement(2927..3235)
                     /locus_tag="LP524_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBL2235760.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQLPLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY
                     "
     gene            3540..4223
                     /locus_tag="LP524_002777"
     CDS             3540..4223
                     /locus_tag="LP524_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235761.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIE
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
BASE COUNT         1259 a          806 c          623 g         1558 t
ORIGIN      
        1 taaaaaaact gattctaaga aaattaaata atttttctca aaatcagttt ttctttgata
       61 aataatattt tcaattattt aatcatttga aaattgttag tcattccaat acgttgagaa
      121 attcttccat ctgtataaac acgaaattgt tttccaccag ccatggtcgt gtttccatca
      181 tcatccgtta caatattaat tgaatagtag cttccatttt catcttgcat tagatctttc
      241 atatattcat ttgtgctaat taaattagta tttgatacat ttttatcagg agaatcatac
      301 aaccataaag ctttattatt tagcacagat tctttagcag cttcccaact tgaaattcct
      361 gaactacttg tagattgtgt cgttgtagtt gttgcttgtt gagtagattc ttctactact
      421 ggtgtttgag gagtttgtcc attcacaata tcactgaaac cttgttttaa ctgttgattt
      481 tgagtttcgt taaagtacag ataattattt tcattacctt gattttgttg cgttaaataa
      541 actttaggtt gtccattttg aaaaccgaaa aagtaaacat gctgccctaa atattctcca
      601 gtttctacat cagaataaac tgctactaaa ttgaaatctg catgaccagt tccatcttct
      661 gaccattgta cggtaactgg tgcttcattg attgccattt tccaatcgcc attaatcacg
      721 gcttgtggta aaggagtgcc atacagactt acattattct gaagattgta gcttttatat
      781 tcttgaccta atgtctttcc ccattgtgtg acaaaagttt ctaatttgga agctttattc
      841 gtatcccaca aagtattctt aacctcttca gaagaagatt cagtgattga acttgcactt
      901 gtttccacag attgcttcat ttctgttgta gtttgttcag ttgattgcgt tttacttttt
      961 ttagtctcaa ttgttgtatc agtttctata gtcgctttag tcctcgtatt ctcagatgta
     1021 caaccagtga ataaaattcc cattcctatt agtaataaca gccatttttt cattatattt
     1081 ccccctttca atttctccct cacgtactta ttctatcaga caattaattg atctaaatgg
     1141 taatctttta atcgattctt tcccaataat gtcgatagcc cgtatggcag cggtctcttc
     1201 cttgaggact ctcagatgga ttaaaactag tagaaatttc ccttccacat cctctacagc
     1261 gataagttct cattattttt gggctattag tattattttt cttagttaga gctatgagtc
     1321 caactatgat taccccaact gtcttaatta cttttccttt atctatattc ggatgcgcaa
     1381 gcatatattt atataaccaa tatccaccgt aaattaaacc acctaataca atcagcttag
     1441 ccatgtactc gtctcctttc tttttgttta attacttttt gaatccatct tgcagagaaa
     1501 ccataaactt ctgtcaattg acggatatct acagtttttt cctgttctat catgttttcg
     1561 attctttttc taactaactc acgatcgtac attttcattg gaaaagaaat ctgttgccct
     1621 cgaaaagcaa gataaaattg atagagcaat tctgaatcca gttccatttc ctcaaaaaag
     1681 tcattcatac ttttataaga gttactcaaa caagtttcat caatctccat ctacacaccc
     1741 cctgttattt tttacataat gaaaatagca tattactggg tacattgata attcatataa
     1801 catgtatcat cacatgtatt tttaaagtca aaaagcatgt aaaactactt ataagtaaca
     1861 tgagaaaata aaatgttatt tttcttgaaa taataattga ctatctgcca attgccattc
     1921 attacgaaca tgaatactta gtgggtggga taacttacct tctgcattta tcaatacttt
     1981 ggtcatcttc ttttgtttcg cactcaattg atcgtaaata gtctttgctt gttcataagc
     2041 cacttgactc gtcgctgtaa tcgaaccaca agcaaaaata atggtattga ttttcttttc
     2101 tgtaaaaaca gtcgttaata tttccaaaga ttgttcatca tttcctcgaa gaaatacccg
     2161 aggacttttc gcttttgtgt acgaaaaaag attcacagca ataaaaccag taaatcctaa
     2221 ttgtcggata ttcttttcga ttaataacat actcaaatca ttcgtaaatg gttctgtatc
     2281 tgttggacga atagtaatga ctactgctaa tttatcttcc gcttcttttc ctttgatcgc
     2341 ccattgtcgt ttgaaaacat agcgatgggt atgatgagga tctcgccaaa cttctgtttg
     2401 tgtggtcact aattcttttt caatcatctc tttttctcct atgctatttt ttgttcaagc
     2461 acttcaatta ccattgccgt tataagtgtc aatactagtt gaaaatattt catattcttt
     2521 ctcctttttt cttcagaatt tgatgaatcg cggtaaggat atcttctaaa catttcgcaa
     2581 tcattctcaa ataatcgatc ttattcatcc gttcctccaa tctgactcca aaccaatcgt
     2641 ccttctacca gtaacccacg aacaaattct cgctggctgg taatatctgt aatgaaataa
     2701 actacatagt cttttccatc ttctggtgcg caataaatat aaagatgtgt catcactaaa
     2761 cgtggtagat gaacatcttt aggaagtgta cctatttcaa tttcctcttt ttccatcgta
     2821 acggattcat agccccactc ttcagaagca gtgaccagct gtgcacactt ttccagtact
     2881 tcgatagccg tttctaacaa gtcctttcgt tcctttcttt tagatactag tattcttctg
     2941 gtaataacat cgtttgatac tcacccgaat caatggccca tattttttga gacactgatt
     3001 tcaaacgaag cttaggtgaa atcagatact cttttctttc ttccggttct tcttgacgat
     3061 gaaccaactt ttgatttcct ttttccgtag aaaattcaaa aatttgaaga tagtctaaag
     3121 gaagttgctt ttgaacgttt tgatcgatta gattccaaca taaagcttgt aattccaccg
     3181 gcaaactgtc cattactccc tttgtgacat aacgttgtcc tttattttga aacatttctt
     3241 ttccctcctt tgctataaaa atagcccaat cttctattaa agaagattgg gcttcatgga
     3301 tataatatat atttgtaaat aaagttaatt cagttataat tatcaaaact acacaattat
     3361 taaagttttt catatctctt tgtttagtaa gtttttctaa atcttaaccc gtggtgctag
     3421 ttaatttttt cctagaggaa aatgtctaaa catgttggta aatcaacaaa aaaacgcata
     3481 tagtagtctc aaaactccca agaaatgagg actatttaac aaactacgat ggttacgtcg
     3541 tgacaactga acattttgac ttagccaatc ctgttactaa agtcgatgat atccccgatt
     3601 atgaaatgta cagtcaaaca atcgatagct taaataagcg ttttggcaat cgtgttttaa
     3661 aaggcatcga aatcggttac atcgcttctg aaaaggatcg gatcatcgac tatttagccg
     3721 ataaagatta cgatttaaaa ttactcagtg tgcatcataa tgggcaattt gattatttag
     3781 atgacgaagt caaagatatg gatcctgcta ttgtgattcc tcagtatttt gcacaacttt
     3841 cagaagcact agttgttata gaagcagatg tctttgctca ttttgactat ggttttcggg
     3901 tatttggtct atccgttgct gaatttaaac aatatgaagc gcagttttta ccgattctag
     3961 atcaagtaat caaaaataaa ttggcattcg aattaaatgc taagagcgct tacttgtatg
     4021 acaatttagc gttatatgaa tatgtcatcg atttatatct ttcacgtggt ggtaccttgt
     4081 ttagtgttgg atcagatggg cattaccttg aacatttccg tttccatttc gatgatcttt
     4141 ttgctctgtt aaaagcaaat ggtgtgacgg aattagcgat ttatcaaaaa ggaaagcgaa
     4201 ttatggttcc attaccagtc taaaaaaacg tcctcagcat caaaag
//
LOCUS       DAFEOM010000131         4112 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.138, whole genome shotgun sequence.
ACCESSION   DAFEOM010000131
VERSION     DAFEOM010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.138"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(39..281)
                     /locus_tag="LP524_002778"
     CDS             complement(39..281)
                     /locus_tag="LP524_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBL2235762.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(313..870)
                     /locus_tag="LP524_002779"
     CDS             complement(313..870)
                     /locus_tag="LP524_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBL2235763.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(883..1071)
                     /locus_tag="LP524_002780"
     CDS             complement(883..1071)
                     /locus_tag="LP524_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBL2235764.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(1084..1650)
                     /gene="amaP"
                     /locus_tag="LP524_002781"
     CDS             complement(1084..1650)
                     /gene="amaP"
                     /locus_tag="LP524_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBL2235765.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            2067..2240
                     /locus_tag="LP524_002782"
     CDS             2067..2240
                     /locus_tag="LP524_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235766.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            2880..3353
                     /locus_tag="LP524_002783"
     CDS             2880..3353
                     /locus_tag="LP524_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBL2235767.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            3362..3589
                     /locus_tag="LP524_002784"
     CDS             3362..3589
                     /locus_tag="LP524_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2235768.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT         1234 a          740 c          617 g         1521 t
ORIGIN      
        1 ttgttcttca tttcaacata agtttttctt caatttgctt ataatttacg tgcaaagaat
       61 gaaacgacta atactaaaat gattgcaccg atcaatgatg gaatgatagc cattcctgca
      121 attgttggtc cccaagttcc tagtaaagct tgtccaattg ctgaacctac taaaccagca
      181 atgatgtttg cgatgatccc cattgatgat cctttattcg tgattgctcc tgcaattgct
      241 ccaatgatac cgccgacgat taatgcccaa ataaatccca ttttaaattc ctcctaactg
      301 acttcttact tcttattgta cacgtggttc gttattttct ttgacttttt gtgcaccttt
      361 gcctacagca gattttgctt tatctgtttg gcgtgaagca aattgtcctg tggattttgc
      421 tgcttctgtt actttgtctt gtactgtttc ttgatctttt tggtattctt cttgtgtttt
      481 gatgtctgca acattcacat tcacttcgat aacatctaaa tgtgtcatat tgtttacttc
      541 agtactgatc aattctttga tttggttgta aatatcttcg atgtctttac cgtattctac
      601 gatcacatct agatccacag ctacttgttt cttacctact tctgtattga tacctgctgt
      661 tacgttatct gtgttgacta atttttcagc catatttgag aaaaatccgc catctaatgt
      721 caatagacca tctacatctt tcaatgcata accgatgatt ttttggatga ctttgtcttc
      781 ataagtcaat tcaccattca tcatgcggtt ttctcctttt tcatttgcca aaactgatcc
      841 atttgttgta ttcatgtttt tgttttccat ctttgtttcc tcctatgatt taaaaaattg
      901 atcaactatt cctgactgtt tcaaataaaa accgattcct gcgccaattg ctgtcatgat
      961 tatgatcaac aatgtcttga aaaatccaat cgttaaccat aaaacagcta gcagtagtcc
     1021 aagaccccca aagataatag ggagcttgta tgctgaaaat agttctttca ttatttggcc
     1081 ctcctattcc actcgagagt gcttgtagtc tcttgtatct tcttgttctt cataaccgct
     1141 gtatgtgact gccactttga ccgtttcttt tgttccgagt actttagtta cttcttcttc
     1201 gatatcttgc atcaatacac ctgttttccc tactaatgaa gaagttcgtt tcaagtctcc
     1261 gttgacatgg atatggattc gatttttcgt tgaacgtatc cgtactttag gtgaatgaat
     1321 gaactcttct tcgtgtaaat gggaacgtac caacccttcg atcgcttttt tgtcgattgt
     1381 tagtttgcca tcttcccgtt tcataacgaa agttcctctt gactttggat agaaaagaac
     1441 gactagcaag atgataatag caagaacggc aaatactaca gctgcccaga aaatatattg
     1501 ttgcatgtag taattcgtta aagtaaagaa gcgaaatcgt tccaattgtt caggcataat
     1561 atacggcgca ttgatcaacg caatcgctag aaatactgaa agtagcagta aactcatgat
     1621 aacaccgatt atttttaacc ccttattcat tttccacctc ctataaactt atttttaccc
     1681 tgtaaataac tctaccgaca atcacagtat agatagcgaa cagttagcat agcatcagta
     1741 aataaagcgc taccaaacat tgctggatca taacggcgtt ttattgccag tctgatactg
     1801 aaataacaat tgcttactta ttctctattt acttttgatt gctggctata cttaaaggat
     1861 attattcaag attaaaaatt gcaaagatta tgctcagtta ttattacaag attctattta
     1921 tttattaaat tttttcggag aattaaaaaa tacaataaat aactttagtt actttaatgc
     1981 gttcttactt tatttttata ttatcatttt tcttattcac ttctttggtt tatactattc
     2041 ttataaataa aaacgaggta tgtcctatga tgaaaagatt gtactattca ttgatcataa
     2101 cgattggcta tttgattgta tcaaatctag gaaatatggt atttggtatc agtaaagaat
     2161 tcagttggac tactacatta tgggaatctc tattcttttt catcttcgta tttttgttac
     2221 aaaattatcg aaaaaaataa acatgtgcca tctatctgtt ggcacatgtt ttaggattta
     2281 tggtactttt tattcggcaa tccattttat ttttctactt cttcttaata cctctaacac
     2341 ttttgattta taaactaatc caatgcatct acttgcgctt attaagctat ttcagctaga
     2401 tagtaccacc ccatgcgtca atgcttgatt ttccttttgt ataacattgt ataagcaata
     2461 aaggacaaat aagtggtgat tacaccaaac cattgaataa gaaaaattct gatacgtaaa
     2521 taaacatgaa cttgaaaaaa ttaatgaatc ggttagctac tacgtagttg tcttttttct
     2581 catacagttc tgttaactta cacttctctt cgctagataa taaaaaatcc tccaactgta
     2641 ttgtccaata taatcccagg catctaaact agcattatag aagagacaca catagaatct
     2701 aaaataactg gttttaaatg tttcttacaa tcaaaaaaag ctgttaaatg cactccacac
     2761 cttcatactc tatcattttt tataaacgat atatttttat tgtttttcga taaaaaatag
     2821 ttgttttaca ataattattc tgctatattt tagctatcaa agataaaaga gggataaaaa
     2881 tgaaatttaa aacattgcta ttaaaatttg tcgttttagg tataagtgcc gtatttattt
     2941 tgttcggcgt acttttttat actcagatcg ctacaagcga aaaaggattc aattttgatt
     3001 ggcagaccac attatttgtt tctgtcattt actttgccct tctcctaggg atcgttattg
     3061 cttatttctt attgaaacta ttgaaaaaaa tcgaagataa ccaagctttt tccactcaaa
     3121 gcttaaacat actaaaaaaa atcgaacgaa ccattttgtt tattggtttc gccttttttg
     3181 gcttacttcc taaatttatg aaagtagcgg atttagacga tgctccaggt ttgatgttgg
     3241 taggaatcgc tgtcgtattc attccttttg cagtatacac attaacaagc gtgctggaaa
     3301 aactgcttga acatgcgatt ttacttaaga cagatcatga tttgacagtg tgaggtgcga
     3361 catggcaatt atcgttaatt tagatgtagt attagctcaa aaaaaaatga gtgtgactga
     3421 attgagtgag aaagtgggca ttacgatggc aaacatctct attctaaaaa atggaaaagc
     3481 aaaagccatc cgtttttcca ctctcgaaaa aatctgccaa gttttggagt gtcaaccagg
     3541 tgatttgttg acctataaaa atgactcgaa tactatacca gaagaataaa ataaagtaga
     3601 acaaacagta aataaaaaat aatctgaatg atagatgtgt gagactttta gaagttattt
     3661 acgtctatta ttcagatttt ttatttcgat tgaataccgt tttatctata aataagatat
     3721 gtactgactc atacaaaggg ggccttttct tctccatgta ctatcacttt tcctgataca
     3781 actggtttcc tgttacgttg tagttggatc tgcttttagt ataatacttt attcattcca
     3841 ccagactgat agcagtttga gcagtctaaa taatcatgta gtattacagg ccattctgtt
     3901 tcataccttg aagtgcttaa aacttcagct aatggcccat tgtgttaact cttactccat
     3961 tcttttttag taagctccca tatatactgt aaagccttct atatattatg tcatgttgct
     4021 ttttaacatt tactatgtag ttttgataaa atgatttgaa taacagtgat ggacaattct
     4081 tttttatttt taacattatt ttgataataa tg
//
LOCUS       DAFEOM010000132         4074 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.139, whole genome shotgun sequence.
ACCESSION   DAFEOM010000132
VERSION     DAFEOM010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.139"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(77..1051)
                     /locus_tag="LP524_002785"
     CDS             complement(77..1051)
                     /locus_tag="LP524_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBL2235769.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLSVPFMDTPLKIQWSIDRITIVG
                     NLKENIYYHTTHDVLILDFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENIAYIEILKW
                     QEGKGRIDFNPNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRNGKLETAYWGSRSSERQIRMYNKKLEQEKKRKIVPKEIVSWWRLELQ
                     LRRGKATDWHAMVYESLDSFASPHYLPADTSVADKMMITALTTEHDYWGQINRKTKYK
                     YRNLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
     gene            complement(1500..2915)
                     /locus_tag="LP524_002786"
     CDS             complement(1500..2915)
                     /locus_tag="LP524_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HBL2235770.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESISAEMRYISKQREK
                     NEREKALVASEKAKIEEEKKSIIDLEKELAVVFQKDPVPLNSFEKLSDGSYRLSSADF
                     VDLYQKASNATNSKKYADMANESLGNTLDKNIALFDKNMALEQENSFLKNQQKVDVSA
                     VVAENKKLKEENRSLRRRLTQLKNAFQKSITRLSIRFGLVEKDMGISEELKILDQEKT
                     LASQQNAPERNIGRSENEMEW"
     gene            complement(3137..3499)
                     /locus_tag="LP524_002787"
     CDS             complement(3137..3499)
                     /locus_tag="LP524_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235771.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAEMGKALRKH
                     FGFESFDDFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPNESDGLLGVIPSKK
                     SQMSESKFTSMFNDVSLR"
     gene            complement(3503..3850)
                     /locus_tag="LP524_002788"
     CDS             complement(3503..3850)
                     /locus_tag="LP524_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235772.1"
                     /translation="MVAVLEMRCNMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERMMDVLNSTINVGLANSSLITDKFVYGKIAETEDESEKEFWLNILTYVMQLNQI
                     GVIYRNKSKGKEL"
     gene            complement(3921..4061)
                     /locus_tag="LP524_002789"
     CDS             complement(3921..4061)
                     /locus_tag="LP524_002789"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235773.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYIYAVAESSGEA
                     ND"
BASE COUNT         1153 a          896 c          576 g         1449 t
ORIGIN      
        1 aaaaaaacaa ccacaacgta atcacctgtt aaatttacca accaattttt cttaccaaaa
       61 tacttttgtt tttctgtcat ttttcttcct cctctgttac gtctagccct agaagccatg
      121 tgtctaattc tttttttaaa tcgtctgccg attctgcaaa ggtttctcgt aaatgattcg
      181 tcaattcatc attttgactt tcttgcttca gcaaatttcg atatttatat tttgtctttc
      241 gattaatttg cccccaataa tcatgttctg ttgttaaagc agtaatcatc attttgtcag
      301 ctacacttgt atcagcaggc aagtaatgag gactagcaaa actatctaag ctttcataaa
      361 ccatagcatg ccaatcagtc gctttccctc gtcttaattg taattctaac cgccaccaac
      421 tcacgatttc ttttggcaca atttttcttt tcttctcttg ttccagtttt ttattataca
      481 tccgaatttg acgttcagaa gagcgagatc cccaatatgc cgtctctaat tttccattcc
      541 gtccataaat aggcttaaaa gaaactggat caactacccg atattgtgta ataaactcat
      601 caggtacatc gataatgtcg caagcaatat ctgcacgtga aaagtgaggt tcgataaaca
      661 aatcatgaat aaaatttttc atggaactag ctaagaattg actaatctta ttcggattga
      721 agtctatacg ccctttcccc tcttgccatt ttaaaatttc aatataagca atattctctt
      781 catgttggtc taacaactgc caaccattat tactaacaga tcttaaataa ccgttacctt
      841 catttaggcg cataagctgt tcaaaatcta aaatcaacac atcatgtgta gtgtgataat
      901 agatattctc tttaagattt ccaacaatgg taattcggtc gattgaccac tgaattttta
      961 ggggggtgtc cataaaaggg acacttaacc gcctgttaga gggggctgtt aatttttccc
     1021 actcatttat gatcgtgcct tttttactca aatttagttc ctcctcaaag aaaaaagaga
     1081 ttggtggaaa gaattttcca gagttcacgt aactctaaaa aattcataac cagcaatctc
     1141 tagtcttgaa gaaacctgaa attattcgta taatgaattt gagttacgga gctagtgtga
     1201 ttagtttccg taaaggtcga gaacgtgtcc agcgttgctc gactttattt ttttgctctt
     1261 ttttcagttt atttatttga taactcaata atagcacttc tctttagcta tgcaaatatc
     1321 caaaaacata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta acgctaagtt
     1381 ggccaaaaaa tggacttgcc attttttagc aaatgtcagc ttcagggatt agtggtttag
     1441 ccgtcaactc cttatcctcg gcacagaccg cctccggtac cgttgactgc accgtataac
     1501 taccactcca tctcattctc agaacgccct atattacgtt ctggggcatt ctgctgacta
     1561 gccaacgttt tctcttgatc taatattttt agctcttcag atattcccat atctttttct
     1621 acaagcccaa aacgaattga taaacgagtg attgattttt gaaatgcgtt tttcagttga
     1681 gttaaccgcc gacgcaaaga acgattttct tccttcagct ttttgttttc agcaacaacc
     1741 gctgacacat caaccttttg ttgatttttc aaaaaagaat tttcttgttc taacgccatg
     1801 tttttatcaa ataaagcgat atttttatct aatgtatttc caagcgattc attcgccata
     1861 tcagcatatt ttttgctgtt cgtagcatta gacgcttttt gatacaaatc tacaaaatca
     1921 gcactagata agcgataaga accatctgat aatttttcaa aactattgag tggcacagga
     1981 tctttttgaa atacaacagc cagctctttt tccaaatcga taatcgattt tttctcttct
     2041 tcaattttcg ccttctcaga agccaccaga gccttctcac gctcgttttt ttctctttgc
     2101 ttagaaatat acctcatttc cgcagaaatg ctctcacgct cttttacagc cgtttggcgc
     2161 aattcagaaa tttcactcat gatttctttg tattcgtgaa tatctttaat tttgttggta
     2221 ccgacaatat cccttttcca accgtaatca ttcattaatc gttctataga gtcaacttct
     2281 tgatttctaa aattattgaa aagcactcga ccattatctt tatcagcgtg tatcccttgt
     2341 tgaaccagtg ctttattaaa gctcggctgc ttttgaacac cccgtttata accttcagca
     2401 accggcacaa tattcaagtg caggtgcgga cttatttcgt cattatgaat aacagcatta
     2461 taaacagcca aattagggtt tctctcttga aactccttgt aatactgaac caaaatttta
     2521 ttggcaatca ctcgattttg ttcagcttgt tgtttcgata tgccagtaga agaaccatct
     2581 gctcgatttg tctggaaatc atctaaaatg cctatttgca caataaattc tcgttgaggt
     2641 tccaactttt tatccttgcg cactttcgct aaatagtcct cgatttttct atcggctctt
     2701 ttttgtttcg cattatattt ttctaccact tcgccaaaca attcctcata tttatctgct
     2761 agattcgtct gctctaaata aatattttca tccgatttcg tgaagtcaat atgcttatgc
     2821 caatcattgg ttttctggct atcagttaac tcacgattat tatgagaaat acttgtattc
     2881 ttcgttcctt ttttaaatga aatactaaac cccaagttaa aaccctcctt cgcgctgtcg
     2941 ctaataaaaa gagtgtaaac ctttctgtta ctctttgtca aagagtaaca ttttcctaat
     3001 tgtgacatgg ctgtcacaat tccaaatgct ctccgagggt attcgctgtc acaagtgaca
     3061 acgaaaaaag gagccgaaag cgtgtcacgc tctcaactcc ttttacgctt tattttgctt
     3121 cacgcaaaat aatcgcttat cgtaaactca cgtcattaaa cattgacgta aatttactct
     3181 cactcatctg acttttttta ctcggtatca ctccgagtaa gccgtcagat tcattgggat
     3241 aatctcgttc gcttggcgct gatttttcat cagcaagcca ctcattatcc actgattttt
     3301 cgtgttgctg atcgtcagaa attgacgata accaccgctc aaaatcatca aaagattcaa
     3361 atccaaaatg cttccgaagt gccttaccca tttcagcatt ttccttctca atcaatcgtt
     3421 ttttctgttg acgaagtttt gcctgttctt ttttcaattt ttcttcttgt gctttaatat
     3481 ctgctaaagt cattcccaag atttacagct ccttgccttt tgatttattt cgataaataa
     3541 caccaatttg atttaattgc ataacataag ttaaaatatt cagccaaaat tccttttcag
     3601 attcatcttc tgtttcggct attttaccgt aaacaaattt atcagtaatc aaagaagaat
     3661 ttgctaggcc tacattgatt gtagaattga gaacgtccat cattctctca agcatttcac
     3721 tataattatc ttcatccggc aaattcaaat aatcagctaa tcgttcatac atcaattcaa
     3781 ttctatcaac tgtctcttct cttaaatcaa ttttatccat gttacacctc atttccaaaa
     3841 ctgccaccat ttccgattct ttaattttcg atacacctaa ttcatcagca agatttttta
     3901 tggtcatctt ttctgacaaa tcagtcatta gcttctccac tactttcagc taccgcataa
     3961 atatatttaa cggcgtgctt agggataata atcggagagt caattttatt atttttcaaa
     4021 atcaattcat attgcgttac atcaagtaac ttaccggaca tttttttgct atcc
//
LOCUS       DAFEOM010000133         3875 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.140, whole genome shotgun sequence.
ACCESSION   DAFEOM010000133
VERSION     DAFEOM010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.140"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            157..2256
                     /locus_tag="LP524_002790"
     CDS             157..2256
                     /locus_tag="LP524_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBL2235774.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2452..3315
                     /locus_tag="LP524_002791"
     CDS             2452..3315
                     /locus_tag="LP524_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBL2235775.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3420..>3875
                     /locus_tag="LP524_002792"
     CDS             3420..>3875
                     /locus_tag="LP524_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBL2235776.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
BASE COUNT         1543 a          646 c          782 g          904 t
ORIGIN      
        1 gcttgagact acgcagtctc aagctttttt ttctatagga aggaaattca ttcattattt
       61 ttaagtaaat cctaaaaata agctattggc gtgacgtata gaatatgata gattaaatgg
      121 tagcgtaaac ctagtaaagg agccgatcgc taagtgatga aaaaatgtcc aaattgcgga
      181 tcgaaaaaaa tggagcagaa tactatctgt ccagactgcg gtcatcaaat aactgcagaa
      241 gatcagcaaa taaaagaatc aattgaagaa acaaatacag atgcagctat gcaggataaa
      301 tcaactgaag atgcagtctt tcctgataca gaattaaacg atccaatcga atggtcggaa
      361 ttgaaagatt tacctctaga atctgtaatg gaattattcg aaaaagcaga accagaagac
      421 gaagaaccac ctgctcacga tcaagtaact aatatagaga aaaatgaaca gaagaaagca
      481 actgttcatg agacttcgga agaatcagga aagaatcaag aagaaacgct tgaatcaaaa
      541 caaaaaagaa gagtagctga actgaaagaa atcgtcgata atgaggaaga aaattcgata
      601 ctatccgctt atatcaaagc acatcgtgaa gatacaaaag aagagcacgc gaaagagctg
      661 ctcaaaatga tcagtgaaaa aattgcacag gaaaacaaag tagagccaga aaaggaacaa
      721 gaaactgaaa aagactctga tagagaaaac gccacaggta aagaaacagt aaaaataaaa
      781 gacgtaaaaa cggatatcac agaaaaaact gaagtattta ctgaaaaaca attagaaaag
      841 gaaaaaacga ataaactgcc ggaagcagag aaaaaagaaa tactcgaaaa cacagacaaa
      901 aagtcaacag agcccgatga aataaatagt gaatccgatg ctgaaaatca atctgtaagc
      961 aaagagggta ctgatcttga agcaaaaata gaaaaagaag agaaccagcc taagctgttt
     1021 gaagcagaag cgaaatcaat aacaatcgtg ccagatactg aaaaaacaca tcaagaatca
     1081 aacttagaaa aaacagatcc agataaagcg caaaagaaaa aatcaaaaaa agtaccttat
     1141 ctcgttttag cagcagtact tctactcggt ggaggaagct gggcttatta tgaccatcat
     1201 caaaaagtag aagccgaagt agcagcagaa gcaaaaagac agcaacaaat ggaagaactt
     1261 catgagaaac ttgctgcctt ttatacagat gatcaccatc aatttattcg tgcagatatg
     1321 attcatcaag atttatcaaa actaaaaaat gatttatcca gtcttaagaa tgaaaaagga
     1381 tattcagagt tagaaacaac ttaccaagat atccaatcta agatccgaga tatccaaaaa
     1441 gtgaatgagt ggttcatagc acctgccatc gtcgatgatc atctagcaga tgacatcaag
     1501 ttgaaagcag ataaagagat ccaagcaatc gaaacggata acacagattt tggaaaattg
     1561 attcaacaag cacaaacaga agctaaaaat caatatgctg aattgcaaac agctaaagaa
     1621 aaaacagcta tagtctttaa agacggaaaa gtagtggatt cagcaacacg cgaccaatac
     1681 aagacggcaa aagaagcagt agaaaaagtg aaaaatacag atctatctgc tcagttgaat
     1741 gaacaattga aacaagtaga taagatgctc actgataaag agaaaaaaga agaagaggaa
     1801 aagaaaaaag cagaagaagc gaaaaaagca gctgaagaac aagcaaaaca agccgctcaa
     1861 gcagcagctg cagctcaagc cgctcaacaa gctgcggtcg cacaagcagc acagcaagaa
     1921 gcggcatcct cgaccccttc ctcttcaaac tcagcgaatc agccaatcaa atctgccaga
     1981 gcaagtgata ttgccgatgc atctaatcca gcatggacgt gggcaccagg tgtccaaagc
     2041 aaagtgattg ctacatgtat ccaacgtgga tatatcgttg aaggaggtta cagattagaa
     2101 aaagccagaa tcgaaaatgg ggaaggctat tataatctat atgccacttc aacaaaatct
     2161 gcattgatga aaggcattgg agaaagtgct ttgccatttt acatcgtaac catcaactgt
     2221 aaaactggat ggttcggagg aaatgggagc aattaatttt aggcaaatga aagaggctgt
     2281 gagcctgccg tttaacaccg tttattcgaa aaaggggagt atgacacaac tgttgtcata
     2341 ctcctttttt cacctttcca aaccaaggct cagctattca agtagataac attatgttat
     2401 gatagcaaag aaatgtatta agaaaacctt tcactataga gggagaaaaa aatgaacgat
     2461 ttattagcaa ctatatttac aggtattatt attgatgaaa atgaaaaaaa ctactttgtc
     2521 caaaaaaacg gacaaacctt tggcttaagc aaatcagaag gtgaacacaa aatcggtgaa
     2581 gcagttgaag gattcggtta ccaaaatcaa aaacatgaaa atcggatcac tacacaaatt
     2641 cctaaaagcc gtatcggtca ttacgctttt ggaatcgtta ctgctacacg aaaagattta
     2701 ggtgcgtttg tcgatatcgg tcttccagat aaagactttg tcgtatctct tgatgaattg
     2761 ccgacaatga cagagctatg gcctaaaaaa ggggatcgct tgatgatttc ccttagagtt
     2821 gatgcaaaag accgtatttg gggcagttta gcagatgaaa aaatattcaa atctttaagc
     2881 aaatatggaa cagaagaaat gaaaaacaaa gatgtgatag gaacagcata ccgcttgaaa
     2941 ttaacaggta cgtacgtttt aacggaagat ttctatcttg ggtttatcca tccttctgaa
     3001 cgatataatg aacctcgtct aggcgagcag ataaaaggtc gagtgattgg tgtccgacca
     3061 gacggtgtac tgaatctttc attgaagcca agagcatatg aagctatatc agatgatgca
     3121 caaatgattt taaccatttt agaacgttct gcagatcagc aaatcaactt tacagataaa
     3181 tcggatccag aagaaatcat gaaagctttt ggtatcagca aaggagcatt taagcgcgca
     3241 ttaggtaatc ttttgaaaca aggattgatt acacaagaaa acggtgtgac aaaactagtc
     3301 aaaaaatcta attgagaatc ccttgcatgt ttttcttaac tagcttataa taattttaat
     3361 taacatagtg ttatatcttt ttttatttgt aattattata aattgttggg gttgagtaaa
     3421 tggtcatgaa tgctatttcg gcgatgaaaa aaacaaaaca gcagctacat gaagcggggt
     3481 ttaagctgac accgcaaaga gaagctactc tgcttgtgct tttggaaaat gagaaagagc
     3541 atttatctgc agaagaaatc ttctttttag tgaaaaaaaa gagtccggaa attggacttg
     3601 ctactgttta tcgaaccctt gaaatcttga cagaattgaa aattgtcgat aaagtaagtt
     3661 ttaatgatgg attagcccgt tatgatttga gaaaagaagg cgccaaacat ttccatcatc
     3721 atttactatg cttgaactgc ggcacgatcg aggaagtaga agaggatctt ttacttgaag
     3781 tggagcaagt agtagaagaa cgttatcatt ttttagtaaa agatcatcgg ctaacgttcc
     3841 atggaatctg tcaggaatgc tatgaaaaag aaaaa
//
LOCUS       DAFEOM010000134         3739 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.141, whole genome shotgun sequence.
ACCESSION   DAFEOM010000134
VERSION     DAFEOM010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.141"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            11..478
                     /locus_tag="LP524_002793"
     CDS             11..478
                     /locus_tag="LP524_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235777.1"
                     /translation="MIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLFAWIVSVI
                     MIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVLRIAFYYT
                     LKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTMDL"
     gene            683..2347
                     /gene="alsS"
                     /locus_tag="LP524_002794"
     CDS             683..2347
                     /gene="alsS"
                     /locus_tag="LP524_002794"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HBL2235778.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            2360..3070
                     /gene="budA"
                     /locus_tag="LP524_002795"
     CDS             2360..3070
                     /gene="budA"
                     /locus_tag="LP524_002795"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HBL2235779.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            3200..3697
                     /locus_tag="LP524_002796"
     CDS             3200..3697
                     /locus_tag="LP524_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HBL2235780.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
BASE COUNT         1233 a          596 c          807 g         1103 t
ORIGIN      
        1 ttgcatcgtg atgataggta tgacagaaga attagcaaaa gaaaaaagtt tttcaccagt
       61 acgtttgttc ataaagacgt ttcgccaatt ttggctaaag ggattttttt attggctatt
      121 tgcttggata gttagtgtga taatgatttt tgattgtttc ttttttatac gtttttctta
      181 tggaaaatgg ttgattccgt tgtttgtttt acttgcttgt ttatcagttt cctttagcat
      241 caactactgg tattttcagg tgagaaatcc agcgtcgaaa ccaaatcaag ttctacggat
      301 agcgttctac tatacgttga aaaaatggta tgtgagttta ctagattttt tactattgac
      361 aagtttgttt ctcttctttt tcgtaaagcc ccaatggtgt attctactag gtccttctat
      421 cgtatttggt ttgatttact tcaacaatcg taaattgatg agaactatgg atctgtagac
      481 tttataaatt gaaactttca attaagaatg acagttttta tgtaaaaact gtcattcttt
      541 ttcatttttt gaaacatatg gctaaacaaa tgaaataaaa cgtgctatac ttttgaatga
      601 aacaggttta gaaactgtat caattatttg tgattatttc taactaataa ttaaaaataa
      661 agcataaaaa ggacgatgtt caatggacga aaaaatgaaa caaggttcga aatctgtagt
      721 agaaagtttg attaatcatc atgttgatta tgtattcggt ataccaggtg caaaaatcga
      781 tggtgtattt aatgaattag aggatcaagg tcctgaatta atcgtgactc gtcacgaaca
      841 aaatgctgca tttatggcac aagcaatcgg acgtattaca ggagagccag gagtagttat
      901 cgcaacaagt ggtcctggtg ctagtaactt agctacaggt ttggtaacag cgacagcaga
      961 aggtgaccca gttttagcaa ttgccggaca agtaaaacga agcgatctat tgaagttgac
     1021 ccatcaaagc atggataatg cagcactgtt ccagccaatc acaaaataca gtgcagaaat
     1081 ccaagatcca gaaacaattt cagaagtgat tgccaatgct tatcgtatgg ctaagagttc
     1141 aaaaaaaggc gcgagcttta tcagtatccc gcaagatgta gtggatgcac ctgtccaagg
     1201 gaatgtcatc aagccattga gcgatcctaa attaggttct gcttctgcag atgatatccg
     1261 ttacctagct gaacgcatta gagaagcaaa actaccggta ctattagtag gtatgcgagg
     1321 atcaagtgaa aaagaaacgt tagccatcag gcaattagtt ggaaagacag cattgcctgt
     1381 tgtagagaca ttccaagcag ctggagtgat ctcacgtgaa ttagaagcgc atttctttgg
     1441 tcgtgtgggt ttatttagaa accaaccagg agatatgctt ttgaaacgaa gtgatctagt
     1501 tattgctatt ggctatgatc caatcgaata tgaagcaaga aactggaatg cggaaaaaga
     1561 tgcgcggatc attgtgatcg atgaagcgcc agctgaaatc gatcctttta tgcagcctga
     1621 aagagaattg atcggtgata tctcagccac tcttgattta ttgacaggtt cattagagcc
     1681 tcaacaagtg tcagaagatg caaaagaata tttagcatct cttcaagcag aattaacgga
     1741 gcgggacatt gttcaatcaa aaggagaagc aggtatcttg catcctttag aagttattaa
     1801 cacgcttcag tcaaaagtaa cagatgatat gactgttacc gtagatgtag gtagtcatta
     1861 tatttggatg gcacgccact ttagaagtta tgaacctcgt catttgttat tcagtaatgg
     1921 tatgcaaacg cttggtgttg cattgccatg ggcgatttct gctgcattgg tgcgtccaaa
     1981 tacacaaatc gtatcagttt ccggcgatgg gggattttta ttctctgccc aggatttaga
     2041 aacggcggta cgtaaaaaat tgaatatcgt tcatttgatt tggaatgatg gacactacaa
     2101 tatggtagag ttccaagaaa aaatgaaata tcagcgggcg tcaggtgtag actttggtcc
     2161 agttgatttt gtgaaatatg cagaagcttt tggcgctaaa ggaatccgag ctacaagtgt
     2221 agaggaatta gaaaaagctt tagaagaagg atttgcgaca gaaggacccg taattattga
     2281 tattccgatt gattaccgtg ataatgagaa attgggagaa acaatcttac cagatcaatt
     2341 ctattaagga agtgtcgaca tgactgaaaa aatcttatac cagcacggga cattaggagc
     2401 tttgatggct ggcttgatgg atggaacaga aacgattgct catatattag aaaaaggaac
     2461 tctgggattg ggaacattgc atgggcttga tggtgaagtc atctttttag atggcgtagc
     2521 ttatcaagga agatcagatg gctcagttgc ccaattagat ggaagtgaac ttacccccta
     2581 tgcagcaatt acagatttta cgccagatac tagtttttct gtctcaaaaa ctgcagatgg
     2641 agaacaattg aaaaaagaca tattagtaag agaagccggg gaaaacctat ttttggcagt
     2701 gaagataact ggattattca aaaatatgca catccgtatc atgccgaagc aagaaaaacc
     2761 ttacagaaga ctagcaaaaa tttcagagtc tcaaccagaa ttccaacaaa gcaatgtcca
     2821 aggaacatta gtcgggttct ttacgcctga attattccaa ggagttgctg cagcgggatt
     2881 tcacttgcat tttatcgatg atacacatac atttggcgga catgtaatgg attttgaagt
     2941 ggctgaagga aaagttgaga ttagccgcat ctcgtccctt gtacagcatt ttccagttga
     3001 tgatccgact tttgttcacg aaacgattga ttatgctgat ttagcagcag aaatccaaca
     3061 agcagaatag aaaaagacag cgagatgcgt tcaaggtgat taacctgacg tatctcgttt
     3121 ttttgaggag gaaacctagc gtttacaaaa aaactgagct ataatcttct ccatagatca
     3181 aaaaggagcg gtttttatca tgtggacaga aaaacagaaa aaagatgcat acgaaatttt
     3241 attattacag caacaaggat tattagaagc agaagataac tggttagcaa atttagcaaa
     3301 ttcatctgca ttgttgaatg agactttgcc tgagactgtc tttgcaggat attatttatt
     3361 cgatggtaat gaattgattt taggaccgtt ccaagggcga gtttcttgca cacgtatcaa
     3421 gatgggaaaa ggagtttgtg gagaatcagc tgaaaaacaa gaaacattga tcgttgatga
     3481 tgtcaaaacc catgcaaatt acatttcctg cgattcagcc gcacaatcag aaatcgtagt
     3541 gccaatggta aaagaaggta atttaatcgg tgttttagat atcgattgcg gagtaacgaa
     3601 agggtatgat gaaatcgatc aagcatttct agaaaaatat gtagatcttt tattagcaaa
     3661 ttttaatata aatgcttttt catttattaa taactaaaat cgtctattga aacaaaaaag
     3721 aagagaaatc aagaataaa
//
LOCUS       DAFEOM010000135         3460 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.142, whole genome shotgun sequence.
ACCESSION   DAFEOM010000135
VERSION     DAFEOM010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.142"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(578..1594)
                     /gene="tsaD"
                     /locus_tag="LP524_002797"
     CDS             complement(578..1594)
                     /gene="tsaD"
                     /locus_tag="LP524_002797"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBL2235781.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(1591..2055)
                     /gene="rimI"
                     /locus_tag="LP524_002798"
     CDS             complement(1591..2055)
                     /gene="rimI"
                     /locus_tag="LP524_002798"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBL2235782.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2062..2604)
                     /gene="rimI"
                     /locus_tag="LP524_002799"
     CDS             complement(2062..2604)
                     /gene="rimI"
                     /locus_tag="LP524_002799"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBL2235783.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(2588..3310)
                     /gene="tsaB"
                     /locus_tag="LP524_002800"
     CDS             complement(2588..3310)
                     /gene="tsaB"
                     /locus_tag="LP524_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBL2235784.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
BASE COUNT          989 a          757 c          594 g         1120 t
ORIGIN      
        1 atatcagggt cactgcttat tttcctccct aaaacttctg aaagatccgg cagttttaga
       61 tcactggaca agactcaaag attttctatg tgtgtttcag ttatgtaata ttcactgttt
      121 gcaaaaaata ctgttagatt aactagatga agcgtcttgt cctcatccga tctagattat
      181 tcgatattcc tcgacaataa acgattactc agtgagggca atcagaggaa cggataatga
      241 taaataagct gtaaaaaata gcaaaataac aataatgatt aaaagtatgt aaaaaatagg
      301 gctttgacaa aaacgtgtca cagccctatt tcgattaaac ggtgtgtacc tgtcaactct
      361 tcggtcacaa gtcataatgt tcataaacat gaggagctgt tttttgaaat tgttccttgt
      421 gaatcagagt taaacgacag gcttacgaac tcttgattaa acggcgttca aaagcttgca
      481 tacgcggtaa taagaacagc caggtcaaaa acatgagaag ccgtttttcc tgggctgttc
      541 ttattacttg atgcagggca gctttttcac aacctcttta ttcagataaa accaagcttg
      601 gatcagcatt caactgatag ctagcaaaat ttcttttttg catttccaca tgtgctgctg
      661 ctccaatcat tgcagcatta tctccacaaa gtcgaagagg gggaatcact agttctacct
      721 cagggagttt agttgccaaa gcatcctgca atccttcacg caacccttga ttggccgcta
      781 cacctccagc tataatcagt tgcttgactg gataattttg acaagctcga agtgttttag
      841 taatcaatac atcgatcaca cttgcttgaa agctagctgc taaatcattt ttgtctagat
      901 cttctccacg ctgttgtgca ttatgcacca aattgataaa tgcacttttt aatccgctaa
      961 aactgaaatc ataattatct tcatggatca tcgctcgcgg aaaatggtaa ttatcttttc
     1021 cctgatgagc tagctgatcg atttcttttc cgcttgggta agaaagacct aatactcttc
     1081 caactttgtc ataagcttct cctgctgcgt catctcttgt ctccccaatg atttcatatg
     1141 atccatcttc ttgcatatag actaattctg tatgtcctcc gctaacaaga agtgccatca
     1201 atggaaactg gaatggttta accagacggg ctgcatagat atgccctgcc atatggttta
     1261 caggaataag cggcaattgg tgtgcccaag caaaagcctt agctgcagaa ataccaatca
     1321 gcaacgagcc gacaagtcct ggtccataag tgacagcgac tgccgataaa tcttctgccg
     1381 acacaccagc ttctaccagt gcatcttcca agcataaggt gatttgttct acgtgatggc
     1441 ggctagctac ttcaggtact acgccaccaa aacgtttatg actgtttatt tgagaagcaa
     1501 cgatgttaga taagatctct gttccatttc taacgaccgc tacactcgtt tcatcacaac
     1561 ttgattcaat cgccagaatc aattcttcat tcatttcatc gtctccgttt ttaactttct
     1621 gctcataatg atcgcatctt ccgtcggatt gcgataatat gatttccgtt tacctaatat
     1681 acgaaacttt tctgattcat aaagttttct tgctgcttca ttagatagac gtacttctaa
     1741 aaaaacatgc agcgaatctt ttatttgttc cgtaatcagt aattgtaaca aatgccgtgc
     1801 atgccctttt ccttgttctg aaactgcaac agcaatattt gtgatctcga cttcatctat
     1861 tactttcgag tatccaataa agccacagat ctggttatct tcagctaaaa gcagataatc
     1921 agagtgaggc tgttggatgt ctgccaaaaa ctgcattttc gtccaaggtg aaccatgtgg
     1981 ataagcattt tcgctaatca tccatacgca ttctgccaag tacgcttcat caaaatcttt
     2041 ttttctgcaa atcaactaat ctcactcatt ttctaaaagt tttatcatat ccaacgcatc
     2101 ttcttttgtt tcagtgtaat atcttttttt gatcgttgtt gcttgaaacc ccaatttccg
     2161 ataaagtctt tgtgcatctt gattactcat acgcacttct aatgacatcg tctcacagcg
     2221 attcattata gcaaagttct cgatttcgtc aataagcagc gaaccgattc gtttacgctg
     2281 atatgccggt cgaacggcaa tattcgtgat atggcaatct aaaccgaacg ctcttgaacc
     2341 gataaaacca atcattcttc cattatcaaa gatacctaga tatagatgag gaagcggtga
     2401 cttcaattct gatataaagg cactttttgt ccatggcaat tcgccattat agacttctcg
     2461 ttcgacatcc agcagctctt tgatatcttc tgtagtcaat tctcttagta cgtaacgacg
     2521 atcgatttgt tcgactgttt tttctgtata aatcatttta tcttgaaaaa agcttctggc
     2581 atacgctcta aattttttca acataacttt cgtctccagg tgtgtgcgtc tccaaccatt
     2641 tttcttctgc ttctactcgt tttaaatagc gaggaagaaa agcttgtttc tctgcaactg
     2701 gttcaactaa agcacctaat tgagccacag ttacaccact tggaatatcc catgctggaa
     2761 ctgtattaat catcgcacta ggcaatattt cctttatttc agagaagaat ttgtgacaat
     2821 cactcccgac gaaataaata gcttcattca gcttttgtag ttgtgtgaac aaatcagaag
     2881 cagaaatatg tacatcttca agtacagttt ctaatctatt ttctttccag cggtatgccc
     2941 cagcgtatac attttttctt cgagcatcaa acatgggcac aataatatta tccacaccga
     3001 tgcaattagc cgcgatggtt ttcagactag atactcctac tagctcttta tctaatgtat
     3061 aagcaatcgt ttttgctgtc gttacaccta tacgaagccc tgtataagaa ccgggaccat
     3121 cagaaaccgc gattcggtca atgtcttttg gacttatttg aagatctgca aacaattggt
     3181 cgattgcagg catcagtgtt acactgtggt tgcgtttgat atttgtttgt atttgtccaa
     3241 gaatttgttc gtcttctacc acaccgatcg ccaaagtttg gtttgcagta tctattccta
     3301 atgtaatcat atatttgatc ctttctgcta tgacattctc gccttccatt gtagcatact
     3361 tttttattct gaggatgaaa ataccaaagg caaaactagt acaataatac atgattatct
     3421 gctggcgggc aaattttgcc ccttcctgaa acacaaaaaa
//
LOCUS       DAFEOM010000136         3292 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.143, whole genome shotgun sequence.
ACCESSION   DAFEOM010000136
VERSION     DAFEOM010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.143"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            37..588
                     /locus_tag="LP524_002801"
     CDS             37..588
                     /locus_tag="LP524_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HBL2235785.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
     gene            1264..1518
                     /locus_tag="LP524_002802"
                     /pseudo
     CDS             1264..1518
                     /locus_tag="LP524_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1588..2100
                     /locus_tag="LP524_002803"
     CDS             1588..2100
                     /locus_tag="LP524_002803"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL2235786.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLC
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            2097..3134
                     /locus_tag="LP524_002804"
     CDS             2097..3134
                     /locus_tag="LP524_002804"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL2235787.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1213 a          555 c          653 g          871 t
ORIGIN      
        1 agcgagcaag acaggaacga gagaacaaac atactcatga aaagactaga ggcttcgacc
       61 tcgaacttta ataataagtt agatgttttt gaagaaaaga ctaagcatgc aagattaaac
      121 tttgaaacaa ctgcaaagca ctatattaaa cgacttgatg aagataattt gaaattagat
      181 tttcaacaag ctattcaaga tgaattaagc gatactaaag acgaaatacg tgaagtgacg
      241 aaacaagcga gagaagaaac aaaagagtat aaagaaatct tagaaagtaa aataaaagac
      301 cataacaaag tggttgataa atcgaataca gcgctaaaag tgatgacaaa aggcgttaca
      361 aatatatttt ttgtgctaat tatttttgta cttgtaatgc ttgtgacagg tccgataggt
      421 catttctttg gtattgaaca tttatatagc tttattaatg gttttattga tgaccacgaa
      481 agtgcatggc gatatttgat gttgatactt tatgcagtac cctatgtatt cttcgctatc
      541 attctgtggc tcatagcatt gttttttaga gtatttaatg acctttaggt agatttatat
      601 ataaaaaggg ctaaatacgc aaaatacccc cttaaaatgc aaaataaatg caaataaggg
      661 ggtaactcct tatgaaaaaa caactaatag tattataaca cgtttttatg ccgagaaaac
      721 ttattggttg gaatgggcta tgtgttagct aacttgttag cgagttggtt ggacttgaat
      781 tgggattaat cccaagaaag taccaactca acaacacata aagccctgta ggttccgacc
      841 aataaggaaa ttggaataaa gcaacgaaag gagttgagta aaatgagtga gaaaacgaag
      901 aaaatcattc gagtatcatt agctttttca caagtagtag tgcaatcatt gatagtttat
      961 tatacttacc agcaatataa attatctaaa gagaattaag ttttgattat gtaataagtt
     1021 ttggtactaa aaaagacttg atctaattag accaagtctt ttgttagtgt tatattaata
     1081 acaaaataaa aataagaagt cgctcactcc ccgaccaaag tttgtgagca aaataaacag
     1141 tcgatgtttg tttcttaatt gtatatcttg atattaaacg atttaagatt attcttcaag
     1201 atatatattc gcaaatgtaa ttggaaaagc gacttcttaa acaaaattaa ggagtcgctt
     1261 tttatggcaa aaataacgaa aaatgagcaa gaaaatttgg tggaaaatta cacaaaaaaa
     1321 ccggcgtact ctaatagccg gttagacgca catttcgtgt gcacttctaa tccgaaattg
     1381 agctttgacg ctatgacgat agtcggaaac cttaataaaa acagcgctaa aaagctatcc
     1441 gattttatga gtttagatcc acaaattaga ctttgggata tacttcaaac aaaatttaaa
     1501 gctaaagcac tacaagaccc cctatgttag gctagttgtc gtataggata ataagttata
     1561 ataaagacta tacggaggat gattaccatg acaaataaaa acaatgaacg ttactctacc
     1621 gaactgaaag cgacagccat caagaaaatg atgccacccg aaaatagggg gattcgccaa
     1681 ttaagtcaaa ctttgggcat tccagaagcg actctttgtt cttggcgtaa aaaagctaga
     1741 attcaaggga atactacacc cggaacgaaa aaaaatgtcg aaaaatggtc aagtgaagat
     1801 aaatttttaa ttgtgatgga atcattctca atgaaccaag tagaacttgc ggaatattgc
     1861 cgaaagaagg gcttatttaa agaacaaatt gaggcttgga agaaaacgtg cttatctgcg
     1921 aatgaacagg aagaaaatcg aacgagagaa cttgcaactg aactaaaaga agagaaaaaa
     1981 caagcgcgtc aattagagaa agaattacgt cataaggaaa aagcattagc ggaagccgct
     2041 gctctcttat tactaagaaa aaaagcacac gcgatttggg gggaccaaga ggaagaatga
     2101 tttcatcttc agatcgcaaa caagctgttg aacttattca agaagccaat cgcaatgggg
     2161 cccgtctcac ctatgcctgt aatgagctga atattagcgt tcgaacctac gaacgatgga
     2221 cgcgtgaagg tacgatagcg catgatcaac gccctttggc gaaacgtcct gtacccaaaa
     2281 ataaactgac ggatcaagaa cgcgaaaaaa taatagaaac cgtttcaaaa aaagaattta
     2341 tgaatttgcc acctagccaa attgtaccaa aacttgcgga ttgctcgatt tatattgcat
     2401 cagagtctag cttctatcgt gtattgcgag aaaaaaatat gcaacatcac cgtggccgaa
     2461 gtcaagtttc acaaaaacgc ataccgccaa gtcaccttgc gacgaagcca aatgaagttt
     2521 ggacttggga tattacttgg ctaaaaggtc caataaaagg actcttttac cggttatatc
     2581 tcatcattga tttatttagc cgtaaaatgg taggctggga gatttgggaa tcagaagaag
     2641 ctcgtcatgc cgaaaaactt attaaacaga cggtgcttaa ggaacgtatc caaggacagc
     2701 cgttggtttt acactccgat aatggcagcc ctatgaaagc cgcgactttt caagtcttac
     2761 tcgaaaaatt aggaattcaa agttcttatt caagaccacg agtgagtaat gacaatcctt
     2821 attcggaagc ggctttccga acgttaaaat atcgaccaga atatcccaat aacggcttta
     2881 aaacaataga tgatgctcga aaatggacga aagagtttgt cgattggtat aacgattctc
     2941 atttgcatag tgggattcaa tttgtgtctc ctgcagcgtg tcatgaagga cgacatctcg
     3001 ctattttaga gaaacgaaag caggtttacg aggacgctaa aaagcgccat cctgaacgct
     3061 ggtcaaaagg tgttcgaaat tggactccac atcaatcggt ggcgttgaat cctttaaaag
     3121 aaacagaaag ttaacaggag cataatcttt caagattatc aaataaccct cgtcacccaa
     3181 taggggaaaa agttattctt cccctattgg ataacgaggc caaccgacca tagggaggtt
     3241 ctgctttttg ataggtggaa aataaaaata cgacaactat attgacaaac ac
//
LOCUS       DAFEOM010000137         3285 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.144, whole genome shotgun sequence.
ACCESSION   DAFEOM010000137
VERSION     DAFEOM010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.144"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            4..588
                     /locus_tag="LP524_002805"
     CDS             4..588
                     /locus_tag="LP524_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL2235788.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(665..1858)
                     /locus_tag="LP524_002806"
     CDS             complement(665..1858)
                     /locus_tag="LP524_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBL2235789.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            1922..2302
                     /locus_tag="LP524_002807"
     CDS             1922..2302
                     /locus_tag="LP524_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBL2235790.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            2376..2738
                     /locus_tag="LP524_002808"
     CDS             2376..2738
                     /locus_tag="LP524_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235791.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         1116 a          539 c          622 g         1008 t
ORIGIN      
        1 aatatggcat tcccacaact agatttaaaa agtgaaaaag gaccaaaagc tgttatcaaa
       61 acaaaccgtg gtgacattac tgttcaatta ttccctgaac tagcaccgaa gacggttcaa
      121 aatttcattg aactttccaa aaaaggttat tatgatggcg tgatttttca ccgcgttatt
      181 cctgatttca tgatccaagg cggcgatcca actggaacag gaatgggtgg cgaaagtatt
      241 tatggcgaaa gttttgaaga tgaattcagt cgtgaactgt tcaaccttcg cggagcttta
      301 tctatggcaa attcaggtcc aaacacaaac ggaagccaat tctttattgt aaataacaca
      361 aatgtgccag ctaatatgct tggacaacta gaaggtgctg gattcccatc agaaatcatt
      421 gaagcgtata aaggtggcgg aactccttgg ctagatttcc gacatacagt ctttggacat
      481 gtactagaag ggatggacac agtagatgaa atcgcaaatg tacaacgtgg tccacaagac
      541 cgacctgtac atgatgtagt gattgaaaca attgaaattt cagaatagtt tggtatggta
      601 cggagtaatt atgaaaaagg acgaaaccta gtaaggtctc gtccttttat tttgaataaa
      661 tgatttaatc gattgtctct aaagcatact tgattcgatt aagtccttcc agtaaagttt
      721 cttctggaca agcaatattc aagcgcatat gcccatgacc ggaaggacca aacgtgatgc
      781 caggatttaa tacgactttt cctttccgaa caagagtgtt ctccaattct tggtcgtctg
      841 aaatataggc ggtaaaatct aaccacatta agtaagttcc ttcagggttc attattctca
      901 ctttcggaag atactgttca aaaaactgat taactgtttg aacatttttt tctagataag
      961 gaatcagttg gcttaaccat tctccacccg tctcataagc cgcttgggtt ccaatcagtc
     1021 caaaagtgtt gatttcgtgc tgttggttca tcactaaatg tttttcaaaa tcagctttca
     1081 tctctggatt tttgataaat accattgaat ttttgattgc agctagattg aaagtcttgg
     1141 ttgcggaagt aaaagcaact agtagatcat ctaaattagg atcaatcgtt agcatggaag
     1201 taaatgtatg atcgaataaa gttaaatctt gatggatctc gtcactaaac agaatcactt
     1261 gatgttttaa acacaattcg cttaactgtt tcaattcttc tttgctccaa acacgccctc
     1321 caggattgtg aggattgcat aaaatcattg cttttacatt gttttcttcg atttttttct
     1381 ccatgtcaga caagtccatg atgaaatgat tatttttttc tagcaaagag cttcgaacga
     1441 tgtgacgttg atttgtttct ataatagagg aaaatggagg ataaactggg tcatgaatca
     1501 aaatagaatc gcctggtttt gtaaaagttt gtactgctgc agctaaactg gttaacacac
     1561 cacttgtaaa aacgatattt tctttcgtca actccacagc atggtgttgg cgttcccaat
     1621 gaatgatagc ctgatatagt ttatcagaca gagtggcata accaaagatt ccatgtttga
     1681 tgtattcact aaacgcttta gctaccccac cgggagacaa gaagtccata tctgctaccc
     1741 acaaaggaag taagtcatcc atttgatatg tctcagcaat tgcgtcccat tttacacttt
     1801 ctgtatcttt tcttgaaatc tgtttatcaa attcgatcat cttaagacct ccagtcattt
     1861 tcgtcgcttc tttttttgag ctatttacag tataatgata aatgcgaaga gagggaagta
     1921 gatgacttat aaaattggac aaataattga aggaaaagtg acagggatac agccttatgg
     1981 cgcattcgtt tctcttgata acgagacgca aggacttatt catgtttcag aagtacaatc
     2041 cggttatacg aaaaatatcc attcattgtt aaaagtcgga cagccagtaa cagttcagat
     2101 catcgacatt gatgaatatt ccaaaaaaat cagtttatct ttaagaacgt tagaaaaaac
     2161 aaatccagct gttccatatc gcagaaaaag gtatttcacg aataaaaatc gtaagatcgg
     2221 atttaccact attgcggagc agttacctat ttggatagaa gaagcattat ctgaactaga
     2281 acggaaagaa aataagaaat aaaaagtgta ttttttctga aaatgtgcta gaatttttat
     2341 gtaagccata gaacaagttg atcgaggtgg aaattttggc aagcaaaaga gttgctggta
     2401 caattatgtt gcatttggag gatggtacta aaaaattttt ggtccattca attgatgaca
     2461 agttggaatt tgcgttagcg gaactttctg aaggaaagac tggtttagcg aatatactta
     2521 atttcttaaa agaaatagtt catatcgatg ttagcaagat tagtttgatg gaattgacaa
     2581 atacacatat taaagaggaa aatgttccgt tgtttgtctt tgaaactgaa caaaagaatc
     2641 aaagagaaga attaggcgaa ggatatattt gggaagaacc aggtcaaatg cgctctgttc
     2701 ttggaactta cgaagtagag ggagtgcctt tcttctaaaa tacagattaa gcgaacaaag
     2761 aagtttgaat ttttttcaaa cttctttttt tattgtttgg tccaaaaaat acaatgaaat
     2821 gttctgctaa agatagtttt ttagtgcatt ttattttcaa aatatgttga aaacggttgt
     2881 ataacgaata ataataattg atcgaaaaat catttcacaa agtaatgctt aatttatagt
     2941 tgaccaagga agaaatacat gataaattta acagcgttga ttctgaagtg gatgttatat
     3001 cacatttcta aaaaatttaa aaaatagtta ttgacatgtt actaacgaca tgataagata
     3061 actgagtcgt taaaaaagaa ccacttgcga agcttgattc tatcgaaaaa aacttcaaaa
     3121 aagttcttga caaataacaa acgataagtt atgataaatg agttgctgaa agatttcgaa
     3181 aaacgaaatc gaaaaaaact tttaaaaaag agttgacaat cgcttgtctc tctgatatga
     3241 tataaaagtt gctacggcag ccaaaaacga atcgggaaac actcc
//
LOCUS       DAFEOM010000138         3280 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.145, whole genome shotgun sequence.
ACCESSION   DAFEOM010000138
VERSION     DAFEOM010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.145"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(366..1484)
                     /locus_tag="LP524_002809"
     CDS             complement(366..1484)
                     /locus_tag="LP524_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235792.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1955..3262
                     /locus_tag="LP524_002810"
     CDS             1955..3262
                     /locus_tag="LP524_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBL2235793.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
BASE COUNT         1032 a          680 c          590 g          978 t
ORIGIN      
        1 tttaaacggt gttccagaag tagcttcttc ggaaataagc cgaaatttca caaaaatttg
       61 agaaataatt tttgtcaatc tcttcttatt tcttgaagct gaccacttct gtcacaacct
      121 cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccacca aacaaaaaat
      181 atgaagagct atttttgctt gctgttctta ttacttgatg cagaacagct ttttcacaac
      241 ctctcataca cggtgtttaa tcagctgacc ttcggtatta gctcaaaaaa cggaaaaata
      301 tgtgaaacta tttttccgtt tgtttctcta atactcaggt caaaacactg atttcacaac
      361 ctcttttaat aacgaagaat cgctgtcaga ctcttttcat ctggcgcatc tttttgatcc
      421 aaaataaaca ctttcccgcc attaatgatc acatcatctg ctaatgcatt caacacttga
      481 cgccgatcat attctgtttc aggatcttca ccgaaaccat cagctaagtt tgacgtagcg
      541 ataaataaat gtgacacttt tccttctttc gcagctggaa cgatatcaac taactgatcg
      601 atgaatttct tatctaagag tttattgtat tcttttgatt cttgagctgc gagttcactt
      661 gacagcttct tcgccccttc ttcgatatct ctaaaggata gctgcgcagg agaagcagaa
      721 atcgcaacgt ctgcacaata atagggattt ttagcaattt ttttgaacat cgtctgattt
      781 tctggtaatg caaataaata aatcggccat tgttcaggat tttcgaaaga atccttcaag
      841 tacgtatcaa cagcttggta atagttgacc caatcgatct ctacttcttc atcttttgga
      901 tttacgccat ggaaagttac tccatctcgg gctccgcctc ctccattaga agagaagttc
      961 aaaccgccac ctgtcagttc agatcccaat gcagtaacga catcaaccgg tgccccttct
     1021 ggtagagata cttcttttac ttgtttgttg ctgacctcat acaatttcat ggagtcgcgg
     1081 ttcaatccca ataaataata agaatagttg aactgcccat ttttgataat agccagtaaa
     1141 taaggaagat cacttacata gtattgatcg tctaccggga tactcaaacg atggatatag
     1201 gtctggtcag cagttataat gaccgctaca ctcgtagttg cactacgcca aaacgaagga
     1261 tctcctaaca aattttcaat tttttcttgg aacggggacc agtcatgttc ggaatatttt
     1321 ttctcaaaac gtttctttgc ttctttcgca aaatttttga atttgatctg atctttttcc
     1381 acatcttgat gggcgacatg cgtattcagc atgatcgtga cgaatggtcc tttagcgcct
     1441 tctgaagtca attgtgtcaa taattcttta tccttttttg ccataaacgt acccctccta
     1501 aaaaaatcat tcctttgttt ctatctaaag tctaccacaa cactattttt ccatacaata
     1561 aaacgtatct aaaaaaataa aacggtttct acggttttaa atttagctat ttttttaagt
     1621 tatcttcata atttcctcaa ttatttagcc ccagtcttca actattcttc atgaaatgct
     1681 gataaactaa gaaaaagaat aaaagaggtg gatcttatgc atttgattat gattttcagt
     1741 ttattaagta ttatcagttt atttgctttc agcgcttccc gcttattaga tgcggaaaca
     1801 cagcctattc ctgtaaaagt acgtaaaaac gaccgctaaa cttccataac gcgcgttctt
     1861 atcctaaagt tttttcagaa acattgaaac tctctcccct ctctgataaa attttaattt
     1921 gtagcgaaac aaattaatta caaggagatc ggatatgtcc ataaaaaaaa cacgtctagt
     1981 cctattattg gctagtctca ttctttgcct tgcttctttt ctaccagctg ttgctgtgca
     2041 agctgaagat acgttcaaag taaatgcaaa agcagctttt gctgtagatg cgcaaagcgg
     2101 taagattctt tatgatcaag atggagaaaa aaccatgggg atcgcttcaa tcacaaaaat
     2161 catcggtctt tacatcgtcc ttgatcaagt aaaagaagga aaactgtcat ggggtgataa
     2221 agtttcgatt tcagattatg cagaaaattt gagtatcacc ccagatttat cgaatgttcc
     2281 tttgcataaa gaaaacacct acactgtcaa agaattattc gactctgcaa tcatccagtc
     2341 agccaatgct tcaatggtgg ccttggcaga aaaaatctct ggcagcgaag ctaaattcac
     2401 tgaacgaatg aaagaacaat tgaaagattg gggaatcaaa gatgcaacaa tcgttaatgc
     2461 atcaggattg aacaactctt atcttggcga aaaccgacca gaaggcactg gagaaaacga
     2521 cgaaaatcaa atgtctgctc aagatgtggc catcgttgcc cgccatttga tcttggattt
     2581 cccagaaata ctggatgtat caagtactac gacccaaatg tttggcgaaa atacgcaatc
     2641 accagtggaa atggtcaact ggaactggat gctcccagga ttcgttaatt acaaggaagg
     2701 cgtggacgga ttaaaaactg gtacgactga gcttgcaggt gcttgtttcg ttggtacgat
     2761 cactaaaaac gatcaacgga tcatcactgt cgtattgaat gcggacggcc atgcggaaaa
     2821 cccgagtgcc cgcttcaatg aaacagaccg tttgatggac tactgctatg acaattggtc
     2881 agaaaaagag ctcggaaaag ctaacgcaag tattcctagt ctaaaaacca ttgatgtaaa
     2941 agacggaaaa gaatcaacag ttcctgttgt gcttaaaagc cctgtaaaag tatgggtacg
     3001 aaacgatatg gatactggca aattaacgat cacgccaaca cttgacaaga agcaagtcac
     3061 tgacaatgag atccaagcac ctatggataa aggtacaaaa atcggtaccg ctacgatcac
     3121 cttggcagac gataaattag gctatttaga agacaacaat gcaccatcaa cagagatcat
     3181 tacggataaa acagtggaaa aagcgaatat attcgtgatt ggctggcgac gcgtcgcaga
     3241 atttttcggc aacttgtttt aattgaacaa taagaaaaac
//
LOCUS       DAFEOM010000139         3218 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.147, whole genome shotgun sequence.
ACCESSION   DAFEOM010000139
VERSION     DAFEOM010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.147"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            50..1438
                     /locus_tag="LP524_002811"
     CDS             50..1438
                     /locus_tag="LP524_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HBL2235794.1"
                     /translation="MYKERDDLNYTLIINKDGNYSWRPLVLLHPILYVDLVNCLTKED
                     HWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILNWWEYFEQKSLAQNIKY
                     QYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLGDKIDKKISGMQHNQTN
                     GIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRYRDDYRIFSNNRNDLEQ
                     IMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEIKASIRMNATNLDGDRE
                     IRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVD
                     IMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTI
                     FGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEGTIKDEEKYIKMSKKIY
                     ITEFDDFNDYPF"
     gene            1776..>3218
                     /locus_tag="LP524_002812"
     CDS             1776..>3218
                     /locus_tag="LP524_002812"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBL2235795.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEEE"
BASE COUNT         1183 a          505 c          582 g          948 t
ORIGIN      
        1 ttcaaaaaaa aaccaaccga aagatataaa agatattgta gatacacgaa tgtataaaga
       61 acgtgatgac ttaaactata cgcttattat caataaagat ggaaactatt catggcgtcc
      121 tctcgttcta ctacatccta ttctatatgt agatttggtt aattgtctca ctaaagaaga
      181 ccattgggag gagttaaaaa agaggtttag tgaatttaaa aaagataaaa atattaactg
      241 ttattctata cctgttgaag cttccaaagg ttcaaaaagg acagatcttg gtgaaacaat
      301 actaaactgg tgggaatatt ttgaacaaaa aagcttagct cagaatatta aataccaata
      361 ttgcatgttt actgacatcg ctgattgtta tccatcaatt tatactcact ctataacatg
      421 ggccatgtat ggtaaagaaa aagttaaaaa tgatgaaaat aagtgttcgg gctcacttgg
      481 cgataaaata gataaaaaaa tatctggaat gcaacacaat caaacaaatg gtatccctca
      541 gggttccgtt cttatggatt ttgtagcaga gatcgttcta gggtatgttg atttgagatt
      601 atcgaaagaa ttaaaaaaat tagaaaattt aaaagattac aaagtactca gatatagaga
      661 tgattataga atattttcaa ataatcgtaa tgatttggaa caaattatga aagtgttatc
      721 agaagtctta tatgatttaa attttaagtt aaattctagt aaaacaaaat tatcagatga
      781 tattatcata gattctatca aaaaagataa gctctattgg gaagaaatta aggcaagtat
      841 aagaatgaat gccacaaact tagatggtga tagagaaatt cgttatagta taaatttaca
      901 aaaacattta ttggaaataa aaaagttagc agataagcat cctaattcag gccaattgaa
      961 aaaagcactt tctgagttta ataaagaacg cattattaaa atcacttcaa agccgaacga
     1021 tcttttacca cttattggta tcctggtaga tatcatgata aataacccgg gttctttgcc
     1081 gcagtgtgtc ttaattatag ggcagttatt aaatttcgaa aatgagagta aggctagaga
     1141 aatagttgaa atgattataa acaaatatgc tttaaagcca aatactgaat ttatgatgat
     1201 atggtttcaa agattgacca ccatttttgg agaaatgttt gatagtgttt tatcatccga
     1261 atttgtatct aaaatcaaag atcctgtctc taatcagatg ttttctgtag actggctaaa
     1321 tgaagatgct aagaaatctt tttatgaagg gaccattaaa gacgaagaga aatatattaa
     1381 aatgagtaag aaaatatata ttactgaatt tgatgatttt aatgattatc ctttttaagt
     1441 gaaaatttag aatgaatata aaaaacatta caatctaatt tttttaaaaa aatgttatat
     1501 cggaagtaat ctcttgattt ctatactata agataagaaa accactattt aacaaggtta
     1561 ttcgaacctt attttaagtt acttattatt taatgtatcg aaaaaggtga aataggtttg
     1621 ttataataag aattaagtaa gacatgaatt gtctaagcaa atgtagctta tccatttcat
     1681 taaaatacat atggtgaaaa aacaagttca aatagtaact aattttttaa aaaactgatt
     1741 tataaaaaat acaacacgaa tgaggaaaat aaccaatgac agaacaaatc gaaaaaaatt
     1801 caaaaagctt atatcaggca ctgtggaatt ccgcagatat cctgcgttca aaaatggacg
     1861 ctaatgaata caagagctat ttactaggct tggtctttta taaatactta tccgacaaca
     1921 tgctgcggta tgtctccgtt ctattggagg aagaaacgga cgacctaaat aaagcacaag
     1981 acctttacgt tgaagcattt aaagatgcag atataaagga tgatctcttg tatgaactaa
     2041 aagatgagtt ttcatatacc atcgcacccg cattgacctt taccgcgcaa gtggcagcca
     2101 ttcatgatgg atcttttcaa ttagaagacc tggtacaagg tttccgtgac attgaacaat
     2161 ccagcgaaaa ctttgagaac ttatttgaag acattgatct gtattctaaa aaactggggt
     2221 caaccccaca aaaacaaaat aaaaccatcg cagatgttat gaaagaatta gaagggttaa
     2281 acatggcggg gcatgctggc gatgtcttag gcgatgccta tgagtactta attggccaat
     2341 tcgcatctga atccggtaaa aaagccggtg aattctatac cccacaaccg gtcgcaaaat
     2401 tgatgacaca aattgtttta caaggaaaag aagataaaaa aggcttttct gtttacgacg
     2461 cgaccatggg ttcgggctcg ttacttttga acgctaaaaa atattcccat caaccgggaa
     2521 ccatttctta tttcggacaa gagttgaata cgtctaccta taatttggcg cgtatgaaca
     2581 tgattttgca tggggttccc attgccaacc aacacttgca taatgcggat accttggatc
     2641 aagactggcc aacagaagag ccaacgaact tcgatggggt gctcatgaac ccaccgtatt
     2701 ctgcaaaatg gtcggctgat aaaggcttct tagatgaccc acgcttctct gcctatggcg
     2761 tattagcccc taaatcaaaa gcggactttg ctttcttatt acatggctac tatcatttga
     2821 aagatacggg cgtcatggcc attgttttgc cacatggggt gctctttaga gggggcgcag
     2881 aagggaagat acgcaaagcc ttgttagaaa atggtgccat tgacaccgtc attggcttac
     2941 ctgctaacat cttctttaat acgtccattc caacgaccgt aattatcttg aagaaagacc
     3001 gtgcgaaaaa agatgtgctc tttattgatg cgtcacaaga ttttgaaaaa ataaaaacgc
     3061 aaaatacgct acgagattac catattgacg ctattttaga ggcctataaa acccgtacaa
     3121 ttaaagacaa gtatgcttat gtcgcagaat ttgatgaaat tgtggagaat gattacaact
     3181 taaacattcc ccgttacgtg gatacctttg aagaagaa
//
LOCUS       DAFEOM010000140         3179 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.148, whole genome shotgun sequence.
ACCESSION   DAFEOM010000140
VERSION     DAFEOM010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.148"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(7..978)
                     /locus_tag="LP524_002813"
     CDS             complement(7..978)
                     /locus_tag="LP524_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBL2235796.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            1684..3063
                     /gene="brnQ"
                     /locus_tag="LP524_002814"
     CDS             1684..3063
                     /gene="brnQ"
                     /locus_tag="LP524_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBL2235797.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
BASE COUNT          894 a          634 c          593 g         1058 t
ORIGIN      
        1 caaagcttat tcttgatgga gatctgcctt ttcataagct ttgatgattt cggcaactac
       61 agggtgccga acaacgtcac ttgcttcaaa attaacaaaa tcgattttct tgatttgctt
      121 taatgttcgt tctgcatgaa caagaccact tgttgtccct cgaggcagat caatctgact
      181 cgtatctcca ttgacaatca tttttgattg ataaccaaga cgtgtcagga acattttcat
      241 ctgagcaact gtcgtgttct gtgcttcatc caaaataacg aatgcatcat ccaatgtccg
      301 cccccgcatg taagctaaag gagcaatttc gatcacgcct cgttccatca atcggttcgt
      361 atgatctaat ccaaaaattt ggtataaagc atcataaaca ggacgcagat aaggatctac
      421 tttttctttt agatccccgg gaagaaagcc tagattttcc cctgcttcca cagcaggccg
      481 cgtcaaaatg attttttgca cttcaccttt tttcaaggca gcgattgcta atactactgc
      541 aagaaaagtt tttcctgtcc cggctggtcc tacgccaaat acgatatcat gtttggctac
      601 cgattcgaca tatttctttt ggccgctgtt ttttacccgt attggttttc cattacggtc
      661 tttgacgatc tcttcctcat acatttcaac aaaataatct aatgtgcctt tttgccccat
      721 tttcagtgcc gtcacgacat ctggtgtact tacatggatt cctcgtttga tcaattcctg
      781 caatgtccga atgactgagg cagccaagga aatctgggat tgttcgccaa tgatctgaat
      841 gatttctcca cgtgtatgga tggttgtttc tgttgtttct tcaatcagtt ttatatgctt
      901 gtcgtgtgca cctaaaagca tgctgacatc atcagcagcc gtcaatctaa tctctaaaga
      961 actagactct tccgtcaaaa atagacatcc cctttttttc gattcattct ttcctatttt
     1021 accatactca gaagaaacta tatagcaaat aagaaaaggt tctaaaagag ttgcttaacc
     1081 ataagtacca agaaacgtta aaaaatagct gcttccattc tctaaaggtc tcgtttgaat
     1141 aaaaaaagct gatcatgtaa gctatgcata agatagagga caagtattcc gtctaactta
     1201 gcaagtattc tctgggtatt tttcttgttt caaaaaaaga aaacaaaata tttgaaatat
     1261 tcttgcattt caaaaaaaac aggtgtatta taacgtcaac aaataaatat acagcaaagc
     1321 gtcgaggaaa acagtaggct attcagcgat ttattcccag tgagtcatgt aagtgagaaa
     1381 tgacaataaa tcgaatagac gaacatcatt tccgagtcaa tgtcgaaaac gtagatcatt
     1441 gtggctgcct ccattatcag gctagcggat aactttatcg aaaagcgtcc gtgaatgaag
     1501 aagctttttt aagcgaaaaa aggtggtacc gttctagaat ctagagccct tttcccaagt
     1561 ggaaaagggc ttttttttat attttaaggt ggtggttttc atggaatctg atattagtgg
     1621 catttctagt ttctgcaacg tttattttgt tactcaaaat gataattatt ggaggaaaaa
     1681 tttatggaag aaacaaaaac ttattcatgg aagcaattat tgatggtcag ttcacttatc
     1741 ttcgggatgt tcttcgggtc aggtaatctg atctttcctg tccacttagg tcaaatggct
     1801 ggatcaaact ggatcagcgc tggtgtagga tttgccatct caggcgctct tttcccatta
     1861 ttagctatct tagctgtagt agtgacaaaa agtgatggtc tgtatgactt agctaaacct
     1921 gtcggaaaat ggtatgctgc cttgttcttg atccttgttc atttaacgat cggaccattc
     1981 tttggtacac cgcggacagc agcaactgct tttgaaatgg ctgctaaacc ctttttgcct
     2041 gaaaaatatg attcattagg tatgttttta ttctctgctc ttttcttttt acttgcttat
     2101 ttccttactg tacatccaac acgtttggtc aaatatgtcg gaaagtattt aaactccttg
     2161 tttcttgcat tattagctat catttttgtc gtttctttat tccaacccat ggggaatcta
     2221 aaccaatctg ctgcagattc ttatcagacg aacgctttga caaatggcgt tttagaagga
     2281 tacaacacgg ttgatgcagt agctttactt gctttaagtg tcacttttgt ccatgcagtt
     2341 cgtgatcttg gttttaaaga taaaaaagtc acagaattaa cagcaaaagc tggtagcctg
     2401 agcgttgcgt tagaagtcat gatttatttt ggtctggtcc ttctcggtgc gatgagtttg
     2461 aataagatgc ctttatcaga aaatggcgga actgcacttt ctcagatcac taatttttat
     2521 ttaggtaact tcggtgctag cttcttaggg gtaatggtca cattaggtgt cttcactacc
     2581 gctatgggac tcgttgtttc ttttgcacaa gatttccata aactgttccc taaagtcagt
     2641 tatatgacat ggctgcggtt aacgactttt gtctcttttg tcgttgcgaa tgccggattg
     2701 gataatatca tccaatggtc tttaccagta ttgatgcttt tgtatccatt gtcacttgcg
     2761 ttaatcttgc tttccttaac agcaaaattc ttccaaaaga gtccatttgt ctatcaagta
     2821 acgatgttct tttctgccgt tccagccgta ttggatatgt tggcaaattc gcctgcatta
     2881 gtgagtcagc aaactgtcgt agcaagctta ctaggatttt atcatcatca cgttcctttt
     2941 gctgcattag gacttggctg gatggtccca acgttacttg gttacgctgg cagcttactc
     3001 ttttattacg cctatcgcct atcagggtat aaacaagaag caaatgaact accagaagaa
     3061 taaccaaatc aaacacaatt taacataaga gatattaaac aggaaaaaaa cagctagaag
     3121 aaagaataag attctttctt ctagctgttt tttagttctt tttttacgaa cacaaaaaa
//
LOCUS       DAFEOM010000141         3161 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.149, whole genome shotgun sequence.
ACCESSION   DAFEOM010000141
VERSION     DAFEOM010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.149"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            553..1725
                     /locus_tag="LP524_002815"
     CDS             553..1725
                     /locus_tag="LP524_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HBL2235798.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            complement(1855..>3161)
                     /locus_tag="LP524_002816"
                     /note="aph(2'')-Ia"
     CDS             complement(1855..>3161)
                     /locus_tag="LP524_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HBL2235799.1"
                     /translation="LKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYP
                     KTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRA
                     YQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSI
                     EIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYS
                     YISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECT
                     IDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNH
                     LLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKA
                     KEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT         1088 a          566 c          408 g         1099 t
ORIGIN      
        1 acactcctct taatatacag tcctattgta taaagtgtta ctatatacat agtaactgta
       61 tacataaaaa aagtcaagta ttagaaaaaa gttcaaaaga tcaagtgtgc gcttcgctcc
      121 cactcccctc gacgttttag tagcctttcc ctcacttcgt tcagtccaag ccaactaaaa
      181 agttttcggg atacattctc cttctcttcc taataatcaa ttaaaatttt aatctttata
      241 tttctgctaa tcactcagga aacagtacag aaaaacaaac acgtatcata gatataaatg
      301 taatggcata gtgcgggttt tattttcagc ctgtatcata gctaaacaaa tcgagttgtg
      361 tgtccgtttt tgggcgttct gctagcttgt ttaaagtctc ttgaatgaat gtatgttcta
      421 agtcaaaaaa ctctgacagc gcctttatat agataaagtc cgtataattg tgtaaaagta
      481 aaaaggccat ataacagtcc ttttacggta caatgttttt aacgacaaaa acatacccag
      541 gaggactttt acatgaccca agtacatttt acactgaaaa gcgaagagat tcaaagcatt
      601 attgaatatt ctgtaaagga tgacgtttct aaaaatattt taacaacggt atttaatcaa
      661 ctaatggaaa atcaacgaac agaatatatt caagcaaaag aatatgaacg aacagaaaac
      721 cgacaaagtc aacgaaatgg ctattatgag cgcagcttta cgacacgtgt aggcacgcta
      781 gaattaaaag tacccagaac acgtgatggc catttttcac ccacagtgtt tgaacgttat
      841 caacgaaacg aaaaagccct catggcttca atgttggaaa tgtatgtatc aggcgtttca
      901 actcgtaaag tatcaaaaat tgtggaagaa ctttgtggta aatccgtctc taagtccttc
      961 gtttctagct taacagaaca gctagaacct atggttaacg agtggcagaa tcgtttatta
     1021 tcagaaaaaa attatcctta cttaatgacc gatgtactct atataaaagt acgagaagaa
     1081 aatcgagtac tctcaaaaag ctgtcatata gcgattggaa taaccaaaga tggcgaccgt
     1141 gaaattatcg gcttcatgat tcaaagtggc gaaagcgaag agacctggac aacatttttt
     1201 gaatacctaa aagaacgcgg tttacaaggt acggaactcg ttatttctga tgcgcacaaa
     1261 ggattagtct ctgccattag aaaatccttc accaacgtaa gttggcaaag atgccaagtt
     1321 cacttcctaa gaaatatctt taccaccatt cctaaaaaaa attcaaaatc tttcagagaa
     1381 gctgttaaag gaatttttaa gttcacagat attaacttag cgcgtgaggc taaaaatcga
     1441 ttgattcatg attatatcga tcaaccaaaa tattcaaaag cttgcgcatc attggatgat
     1501 ggattcgaag acgcctttca atataccgta caaggaaatt cccacaatcg actaaagagt
     1561 accaatctaa ttgaacgact gaatcaagaa gtacgcagaa gagaaaagat tattcgcatc
     1621 ttccccaatc aaacatcagc caatcgctta attggagccg ttcttatgga cctacatgat
     1681 gaatggattt attcttcaag aaaatacatc aattttgata agtagaaatg gtaaaaacat
     1741 tgtatagcat tttacacagg agtctggact tgactcactt cctttattat ttttcatttt
     1801 tttaaactat ttaatactaa tgtcttttat aatagctttt catatattat ataatcaatc
     1861 tttataagtc cttttataaa tttcttttct accattttcg ataaattcct gtttaatatt
     1921 tttaattcca taaacaatag tttcaatagg ataatattct tcaactatat cttgatattc
     1981 ttttgctttc tcaatatcta tatttccata cattcttaat atatcttctc caaaatttgt
     2041 tcctatttct tcttcactat cttcaagtaa gtatataaaa tcacaatatt catctataat
     2101 tccagaatct ccaaaatcaa ttattccagt taatctatta ttgccatcta acaatagatg
     2161 attacaacta aaatcattat ggcataaaca ctttttaccc tcaaaaactg ttgttgcatt
     2221 tagtctttcc ataaaacttt ctatataatc tttttctata tcagttaaat cattataaat
     2281 agtttcacgc aacaatatat actcttctaa tacattttgt ttattatcaa tagtacattc
     2341 actaatatct gtataatcta aaccgtgcat ttgtcttaaa aaactggcaa tatctcgttt
     2401 taacaaattt tgttcttctt ctgacatagt agaataaatt tctggtgtta aaaaagttcc
     2461 tttaatttct ttataaccta gtatagataa ttcatcacta atatacgaat attcaatatt
     2521 aggaattttt acattagttt ctaaatttgt atttaaaaaa ttatatattg ctttttcttt
     2581 tgcataacct tttttcttat tagtactaaa ttttgtttta aaaatgtatt cattattaac
     2641 taaatatgcc acactatcat aaccactacc gattatttca atactatcta ctttgaaatt
     2701 atcaaagtaa tgctcaatta aatatttcat tgccttaaca tttgtggcat tatcatcata
     2761 tctatattcc attaaataac aatcttcttt tttgccctcg tgtaattcat gttctggcaa
     2821 atcttcaata attctaaaac cagatttttg gtatgccctt attgctcttg gattattttt
     2881 atgagggtct aaaataactg cattagcatt tctttctttt ttcaaaaatt caaaaatcaa
     2941 tttaatatat cttgtaccaa ttcctttact ccaataattt ggctctccta taaattgatc
     3001 cataccatag actatctcat cagtttttgg ataatgataa tcagtatata actcatcata
     3061 cattttatat atttgtccat atccaatagg aacattgtta tattcaataa ttactctaaa
     3121 aacttcatct tcccaaggct ctgtataatg tttttttaat g
//
LOCUS       DAFEOM010000142         2953 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.150, whole genome shotgun sequence.
ACCESSION   DAFEOM010000142
VERSION     DAFEOM010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.150"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            89..406
                     /locus_tag="LP524_002817"
     CDS             89..406
                     /locus_tag="LP524_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBL2235800.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(497..862)
                     /locus_tag="LP524_002818"
     CDS             complement(497..862)
                     /locus_tag="LP524_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235801.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1069..1551
                     /locus_tag="LP524_002819"
     CDS             1069..1551
                     /locus_tag="LP524_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235802.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1748..2638
                     /locus_tag="LP524_002820"
     CDS             1748..2638
                     /locus_tag="LP524_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HBL2235803.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1008 a          460 c          566 g          919 t
ORIGIN      
        1 ttttaaaaca tcacaaggaa aaacttaact atggtattat ctaaacataa ttttttcggt
       61 atttaatatt taattaggag gtaatatttt gcttttttat agtaatttta tcttaatagt
      121 agcgatatta ttacttctga atatatggat ttttgatagg agtagaaatt caagtatagg
      181 ttttagaact aaacgaagtt tgtcttctaa aaaaaattgg gtatactcac aaactatttt
      241 ttatggagga attgttttaa ttagtttact ttcttcaaca ctttattctt tgaatattat
      301 agatgtttcc acctcaaatt caatatctat tattggaatc attattgctg caattattac
      361 acaactgttt ttagtgtttg gagagaagaa aagaagtaaa aaatagaaaa actagtgata
      421 gttttggtga tataagaaaa acatatatgt ataaaaagaa gaaagtagct tctctttgta
      481 catatatggt ttgtttttat ggatgccgag cttttttata agataaggct accgcccata
      541 acgacccaag gatttgtgga actaagtaaa tacctgcaag aagcaagatc agctgggtcg
      601 gttttaaagg atgatagaag taacgccatt cagtaaaatt ggcgcaaaat aaataggaag
      661 tgacagtaga tataatattg gcaatgaata aagatataat acgcctgttc ctttgaaaat
      721 aatatccagc aaaaagagat aaaaatatca atgtgatgaa cccgcttacg gaatgataac
      781 gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa agataaaagc aacggaaggt
      841 ttttccagca agtttttatc atctttcatc accacctaac aagtgttatt attttgttag
      901 ttttattgta aattgaatcc tgcaataatc aattactttt tcattatttc tagattaaat
      961 gaataaactc tcatgccatt atctctaaaa aaaagttttt ccccctaatt tttttagata
     1021 gaaaattgta tagtatgatg agagaaaaag tagtttgggg gaacgttaat gagaagaaaa
     1081 gcagtaatcg atcggcttta tttatttttt tggttttttc ctatcgtttt gctacctttt
     1141 acatgggtaa atgctgttgt acaaggtgcg gagatattag gcctgatcca aggaagcgga
     1201 tttgtggttt ctaggaattt tccagctttg attgttttct atttagtgat aagtgtaggt
     1261 ctcttattac caaaatctct gatcaatcac tacaattggc agctgcttta cttttcttgc
     1321 ttattagtat ttacatctac gttttctttg ctgcctcgct tgattatcgg ccctaatggt
     1381 ttgagcgaga gtggattgaa ggatttagct tattcctcca tgtatttctt gacgatcaaa
     1441 ccatggttct atttagtaat tgcctcattt attcttgcaa cattttttta cttctggaca
     1501 gaaatcagga taaaaagtga acgatctgta tctataaaaa ataactattg atggtggatc
     1561 atcagtaaaa aaacatatga gctgcgtaaa atgcatgttt ctcatatgct ttttttatta
     1621 ttatgtaaag aatgcgaaaa acgtttacat gatttttaca taacttaact atatttttac
     1681 agcaaccata cgagggattt acagtcgact gataaacttt agttgtaaat aagaaaggag
     1741 caataaaatg aaaaaagtat tgtttactct aggtatgatg ggaattcttt tagccggatg
     1801 cggctcagga tcaggaaata cagatggctc tgccacacaa aataattcaa gtgactcaaa
     1861 tgaacaagta aaaatcgttg cggtaggttc gacagcatta cagccgttag tcgatgcagc
     1921 acaagaaagc tttgtacaag aaaatccaaa ttatcaaatc tctgtacaag gaggaggaag
     1981 cggaactggt ttgagccaag tcgaagctgg agcagttact atcggaaact ctgatgtatt
     2041 tgcagaagaa agagacggag ttgatgcttc aaaattagtg gatcataaag tagcagtggt
     2101 cgggatggca ccaatcgtca ataaagatac agatgtcaaa gatatcacca aacaggaatt
     2161 gattgatatc tttacaggaa aaatcactaa ctggaaggaa gttggcggga aagatcaaaa
     2221 aatcaacgtc gtgaaccgtg caaacggcag cggtacccgc gcgacattcg aaaaatgggg
     2281 actggatggt gctacccctg tgcagtccca agaacaagat tcatcaggaa cagttcggca
     2341 gctggtaagt caaacaccag gagcaatcag ctatttagct ttctcatatc tcgatgattc
     2401 cacacaagca ctaagtatcg acggtgtaga accaaaagaa gagaatgtag cagataacag
     2461 ctggggaatc tggtcatatg aacatatgta cacgaacgga aaaccttccc ctgaagtcca
     2521 aaaattctta gactacatga tgacagaaga aatccaagaa ggacctgtca aagaattggg
     2581 ctatttacca atcacaatga tggaagtaga acgtgatcac gaaggaaata tcaaataact
     2641 agcagataaa catgccacgg tagtcgttac tattaaggaa aaatcaacta tgtttcccca
     2701 catatacatt tttcctttaa ttgtgaaaaa tagttcctca tattttgccg ttttttgagc
     2761 taataccgag ggtcagctga ttgaacaccg tttattcgaa aaaaagagct acgacataga
     2821 actgtcgtaa ctttttttgg ctcttcgtca aatcgtgttg gtgaagacaa atttgaataa
     2881 aacttagacc gatatgcggc gcgttctgtg cgccgcatat ttttttagtt ttcaaagatc
     2941 agaccttgtt gta
//
LOCUS       DAFEOM010000143         2878 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.151, whole genome shotgun sequence.
ACCESSION   DAFEOM010000143
VERSION     DAFEOM010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.151"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(1321..1839)
                     /locus_tag="LP524_002821"
     CDS             complement(1321..1839)
                     /locus_tag="LP524_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HBL2235804.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT          960 a          521 c          405 g          992 t
ORIGIN      
        1 tttttggcaa taattttgca atcaagccat ttacagatgt aatgcgcgga tacttctgct
       61 aagattgcaa aaaacatatc gaaaaggtaa ttcataatac aacctcctcc ccttgccata
      121 ataggccggg gtagcagaca gcataaatat tataccataa ctgaaaattt cattttagta
      181 catttcaata tattgatgaa ccagcacaat aaaacatgcc atgactaaca gaaaaaaact
      241 tattaaacca gatataactt gtaaacatcc aactatatag aaaacgtaaa ttttctatat
      301 agttggatta agataacgac tatttatttt atcatatttt tactataccc tatactttta
      361 cttggtatat ttatgaataa tcgcaaattt acccctatac tactagtttg taagttagat
      421 taataaaaat actaaagtta tttgttcttt tattaaatac acctacaact tataaattga
      481 tggtatttag ataacacgaa agaataaaaa taacagctga tagtaaagtt tgttgacgat
      541 tgctactcaa tcgaaagtga gttagtgcgt atgaacttgc ttctgaaaac ttatgaaatg
      601 aaagaccttt tacatcttgc agaaatcggt actgggatta aactaagcaa taaaaactaa
      661 tgttcccctc attctttgaa cttctactat ttgatttatt tattctgata aatgacgatt
      721 tcaccttcag gatcttcaaa tcccgtatag aatttattta agatctcttc agtatcagta
      781 ataggatagg cgataggcca catacccttt gatggttaga ataaactgct caaaatctgt
      841 atcgtatgtt ggctttagca tattaacaaa ggctatctcc aaattataaa ccataagcgt
      901 tgaatcgttc ttaacaattt caattcttcc taacccaaag tcagaagata taattaccgg
      961 acttgcctta cccttcatgt gggttgttga gattccatat ttaaagggaa ttgtcggtac
     1021 tatcggcggt ctgaaaaaca tcccaaaaat aaaaaaatct ttcccctgct agtctcctaa
     1081 gtccctttgt agcggatatg gcaccataat ctcattttat cgaagtgagt catggtttat
     1141 ctgttggcca accaaaattg atacaatcta acgatgcacc ctctacggca ttctagggga
     1201 gtgcacttgc attcacccaa taaaaaccgg cagttagcaa agaacttatg tagtcgtatg
     1261 ttcttactgt taacatacaa gaggtctcct ttccacaatt gcttagcagt aaacgggaat
     1321 ttacaaagct aatctcttcc attttcttat ctttgttcta aaagtcccaa atggagcaac
     1381 tgtatttaca tgaataaatt tatatacttc ccatacagcc gttttggttg cctcgtctgc
     1441 ccattttctt ttgtgtgcag tgaatagatc ttcatcactc attgaatcaa tcatcatgtt
     1501 aatatcatca atattacgat tgagaatttc ctctaattct ttcaatggta gatgtgcata
     1561 ggtatttgta aaccacttat ataactctcc taactgattc catttaaatt gatcagaagg
     1621 cgttttaaca gataatccat ttctttcatc attttcccat tttagaatta aattcgtcca
     1681 accaacttga tatgctaaat tttcagcagg tgttctatcc acttcctcaa ctcttttatc
     1741 cttcaattct tcaggaatag tttcaaattc taaaatatat ttagtataag tcctatttat
     1801 ttcttttttt aattcttctt tatttttata tgttctcatt atattacctc cataaattcc
     1861 taattgtctc ctttaattca tctataattt catccacgat attttaatcc gttctacaat
     1921 gcatatctga tatgcattga ctgtctaact catatgtgaa tgatttataa aatatcccaa
     1981 cgtctcatct aaagaaatgt tgatcgtcag caaggagcta aaaaatacga atatatttct
     2041 tttgtagtaa aatcggactg gtttgcatat cattttcttc gtaaatatca aaatcatgtt
     2101 aactactatc aattactaat aaaaactacc actttaagcg ttaagaactg ttaagtttag
     2161 ttaagtgagg atagttcaca aaatccattg ttaaacttca cgaaagtggt aaaactcaaa
     2221 atgctttata taggattaca gcatcgctct ttcttcgatt gcgcgttggg taaaaaatat
     2281 tatgaagtaa aaattgacga tgacacgttc cttacggctc atcagatgaa acagctacaa
     2341 aaacgaaatg atcaactcga agaaaattta attctaaaaa agcgattgcc atattcacgc
     2401 ctcgctcgac gaactattaa aggtcaagat ttacctgaag atcatccttt ttaggaaatt
     2461 gaagcttttt atgttgcaca atgcatttat aatactacaa tgatgttttc accagatatt
     2521 cttctagggt gcggtgtaat gaaacagata cacttgcaaa agaaaattca ccgcgaattt
     2581 actaaaatta tgaataatta tgttaatctt ccgaaatttt tttccaaaat taagggatca
     2641 agctggtgtt atagattgtt taaggttggc aaaaaaataa cttcttttta aacaattata
     2701 gaaaaccgat actctgttaa gttggttgac acggtgtcga tttttatgac tttcttattc
     2761 tgaaacttca ctaacggaca gtacctaaaa agtctatagt atggacgttt acgagtacta
     2821 ttttatcaaa aatgctgctc atttcaactt atatgtttta gtgaactacc cccactta
//
LOCUS       DAFEOM010000144         2731 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.152, whole genome shotgun sequence.
ACCESSION   DAFEOM010000144
VERSION     DAFEOM010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.152"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            179..775
                     /locus_tag="LP524_002822"
     CDS             179..775
                     /locus_tag="LP524_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBL2235805.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="LP524_002823"
     CDS             899..2698
                     /locus_tag="LP524_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBL2235806.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT          776 a          468 c          516 g          971 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctataatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac a
//
LOCUS       DAFEOM010000145         2636 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.153, whole genome shotgun sequence.
ACCESSION   DAFEOM010000145
VERSION     DAFEOM010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.153"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            106..1305
                     /locus_tag="LP524_002824"
     CDS             106..1305
                     /locus_tag="LP524_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBL2235807.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(1332..2600)
                     /locus_tag="LP524_002825"
     CDS             complement(1332..2600)
                     /locus_tag="LP524_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HBL2235808.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
BASE COUNT          789 a          573 c          468 g          806 t
ORIGIN      
        1 aaaacatctt tccatcggaa agatgttttt ttgctttttc tagtttaaat aacagctttt
       61 ttcatctatt ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga
      121 gatctgatct ttttagttct ctatcttatt ggggtattct ctatcgttta ttggcgaaaa
      181 cagaagaagg atccttcctt gttcttttac ggcagtacgt ggacatttac ggtaatctta
      241 acttattttg tcgtgttgga aattttttat tcgacctttt attttttgat tccattgttg
      301 ttctttagta tctttgcttt ttcttatttc acagaaaaac gaaggctact gaatggtctt
      361 ttattcaatg ttttactcat cagtttcggc atctacttgt ttgtcttgct gtatgaaacg
      421 caaaatattt ttcttggcgg actgatcgcc ttgatcacga ttccattgct acttgtcctt
      481 ttatttggta tttatggatt gattgttttt cttttttgga acggggtcac agttttacgt
      541 agagaatcgc attcattagc taatctttta acgttgatac tagccatttt tcttacactg
      601 tttcttgttt ttgacttttt cttgttaaaa tatttgccgc aatggataaa tgctttgttt
      661 ttctgcgttc ccctgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc
      721 tcattcttgt atcagttcaa ccggcctcga tacaatcaag acttcatcgt tgtgttaggc
      781 gctggattga tcaatggaga aacagtttct cctcttctag ccaagcgaat caataaggca
      841 attgcttttt atcgggctca aagccgtgct acattaaatc cgcccatctt attgatgtct
      901 ggcgggcaag gagcagacga aaaagtacca gaggccattg cgatgaaaca atatgccatg
      961 gaacaaggaa tccctgaaag agacattctt gtcgaaacca attcgacaac cactttggaa
     1021 aacatgctct attcaaagga aatcatggat cagcaaatga aagggccata ccgcgcgatc
     1081 tttagttcca acaattacca catttttaga gccggtcttt atgcgcggca agccaaattg
     1141 aaagcaaatg gtattggtgc aaaaacagct ttctactact tgcctaatgc gtttttaaga
     1201 gaatacctcg ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt
     1261 ttcgcaggct ccgcattctt atcgctgatt actttacttt tctaaaaaaa caaggtgtga
     1321 caaaacatat gtcacacctt gtttttttct ttcattctct taactattgg caaaaagagt
     1381 atcccttttt tgaccgctac ttgctgagca gaataaatac cgctgttgcc tgaacacata
     1441 aagctgatca agcagatcat aaagaagtat actgccgctt gtgaaccgaa caattcgatc
     1501 cccatcacaa agcaggcaat cggtgtattt gtcgcaccag aaaatactcc gataaagccc
     1561 aatcctgcaa gaaaaggaac agaaacatgg agcaaaggcg ctagtgctgc accaagcgtt
     1621 gcaccaattt caaacaatgg tgtcacttct cccccttgat atcccgcgcc taaagacaaa
     1681 actgtgaaga ataacttccc aataaaatca tagacatgtg cctgtccatt aaatgattct
     1741 tgaagtaacg gaagactcaa tccgagataa cgttgtgttt gcaaaatcaa tgctgctgcc
     1801 acaactacag ctccacctat cgtatttcga caaacaggat gaggaatcca ttgtgcatat
     1861 cgcgctttta aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc
     1921 ccagcaacaa aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg
     1981 taatgtagat gagaaacacc atagctttct gtcatgaaat ttgcaaacag tgcagcaaag
     2041 aagctaggga agatcgcttc tgttcggact ttcccgattg ccaacacttc taatccaaat
     2101 aatgttccag ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata
     2161 atcaagattt cccgttctga tttatccaat cggaacaaac gtgccgtatt atctgctaac
     2221 gctcccccca tttggactgc tgttccttca cggccaacag atcccccaaa taaatgggta
     2281 gttatggttc caaacagtgt aagtggaata agccgcaacg ggattttttc ttctccacca
     2341 tttccttgat cgatcactaa gttgtttcca cgactagaaa gtccgccgtg atacgcatag
     2401 agataagcaa aaagcgcacc actaactggt agtagacaaa ggagccaagg atgtgatagt
     2461 cggatatctg tcacaactgt caggctattt aagaaaaaag cagacaagct ccccatgaat
     2521 ataccgagaa tcgctgtgat caccagccac ttcagcatat acactaaaat gcgtactgga
     2581 ctcggtaatt gtttttccat aaaaaattcc tcctcaataa aaaaacgcct actaaa
//
LOCUS       DAFEOM010000146         2577 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.154, whole genome shotgun sequence.
ACCESSION   DAFEOM010000146
VERSION     DAFEOM010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.154"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(<1..718)
                     /locus_tag="LP524_002826"
     CDS             complement(<1..718)
                     /locus_tag="LP524_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235809.1"
                     /translation="MRSSKYTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVL
                     KGIEIGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFA
                     QLSEALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKS
                     AYLYDNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKAKGVTELAI
                     YQKGKRIMVPLQSKKTSSASK"
     gene            1461..2015
                     /locus_tag="LP524_002827"
     CDS             1461..2015
                     /locus_tag="LP524_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL2235810.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            2269..>2577
                     /locus_tag="LP524_002828"
     CDS             2269..>2577
                     /locus_tag="LP524_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235811.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     L"
BASE COUNT          966 a          332 c          429 g          850 t
ORIGIN      
        1 cttttgatgc tgaggacgtt tttttagact gtaatggaac cataattcgc tttccttttt
       61 gataaatcgc taattccgtc acaccttttg cttttaacag agcaaaaaga tcatcgaaat
      121 ggaaacggaa atgttcaagg taatgcccat ctgatccaac actaaacaag gtaccaccac
      181 gtgaaagata taaatcgatg acatattcat ataacgctaa attgtcatac aagtaagcgc
      241 tcttagcatt taattcgaat gccaatttat ttttgattac ttgatctaga atcggtaaaa
      301 actgcgcttc atattgttta aattcagcaa cggatagacc aaatacccga aaaccatagt
      361 caaaatgagc aaagacatct gcttctataa caactagtgc ttctgaaagt tgtgcaaaat
      421 actgaggaat cacaatagca ggatccatat ctttgacttc gtcatctaaa taatcaaatt
      481 gaccattatg atgcacactg agtaatttta aatcgtaatc tttatcggct aaatagtcga
      541 tgatccgatc cttttcagaa gcgatgtaac cgatttcgat gccttttaaa acacgattgc
      601 caaaacgctt atttaagcta tcgattgttt gactgtacat ttcataatcg gggatatcat
      661 cgactttagt aacaggattg gctaagtcaa aatgttcagt atattttgat gaacgcatat
      721 gtagtcctca tttcttggga gatttgagac tactatatgc gtttttttgt tgatttacca
      781 acatgtttag acattttcct ctaggaaaaa attaactagc accacgggta tactactata
      841 ttttaggttt catattatat aaattaataa tatactacca tttgttggta gtataattta
      901 aaggtaataa gttattcttt tattactatg tttgggtttt atagcaagga aagtttaata
      961 agagtttgga tctagcaaac aatattaatg aggtcattaa atcttattat ctttagtgat
     1021 ataacgaaat atctacacag acaaaattat gatcttgcgt aaagttataa aatatcaata
     1081 aaaatatttt attaatgaaa agcttggtag cacggttatt catatgctaa tttatttcac
     1141 tttatagtaa ggggagttct tagtatattg ttcaaactta aatttcatat aatttaatta
     1201 tgtaaattag ctattctaag aacttctacg gataattgcc cgtctaaaat ttaaataaga
     1261 ggttagaatc ttcatataag taaggttaat ttttttgtac aaaaaagtcg gaatattctg
     1321 agttactgaa atgatataaa atcataaact aaaccccttg attttcttca cttttaacag
     1381 agtatatata taagttcatg attatggtaa aatatatata taatcatgaa ctcagaatat
     1441 ttttaagaag ggaaacaata gtgagctata atgtacaacc gttaaggaca aatgaagaga
     1501 tacaagattt tcagttttgg ctaagaagaa caagtaatcc agaaagagat agttttttat
     1561 ttttgtttgg aatcaataat gggttacgta tgtcagatat tataggatta aaagttggag
     1621 acatacgagg taaaagtaag cctattattg ttgaacataa aacaggtaag cgaaaaccga
     1681 tttttataga taatttgaga gaagagattt tattatatac tgaaggtaaa gaagaaaatg
     1741 attggttatt tccaagtcgg caacaaggtc gacatataac aagggatcgt gtctatcaga
     1801 tatatgcaga tatagcagaa aaattaggaa gagatgatat aggaacacat acattaagaa
     1861 aaacttttgg gtatcactac tacaagaaaa ctagagacat tgctacctta atgtttattt
     1921 ttaatcatag ctcgcaagca attacaaaaa ggtacattgg tataactgaa gatgaaatag
     1981 gggcatcgtt acgtggtttt aaattgggag gctaattgag atattaggat atattttttg
     2041 tgtataatga aaacttagaa ggtgagatta atgaagataa ctgaattttc ggaagtattt
     2101 aaaacgttta gtaacattta caatagaata ccagagatag aacaaaatgt tgtagttgat
     2161 aatgattatt atatagagaa tgattttaat aaaccaagaa aatatgacaa cctatacaat
     2221 tttatacaca atagaacaac aataaataac aacagtaaca ctcgagtttt gattaattca
     2281 gcatattttc atattaaata tttaaagaaa tatgagtatg aagaagttgt attagattat
     2341 gggctaaata gacagtcatt tttaatttat gcaataaata acggaaattt gccaagcaat
     2401 aaaaagaaca taattattga aacatatgcc tatctggagg ggggatctaa aaaactaatt
     2461 aagtcatgta atatagagta tcaattatca gtaggagaaa ttaaatgtgt tttcgaagaa
     2521 gatttagaac ttgacttaaa aaaaattttt gccagcattc atgattatat aggtttg
//
LOCUS       DAFEOM010000147         2486 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.155, whole genome shotgun sequence.
ACCESSION   DAFEOM010000147
VERSION     DAFEOM010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.155"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            1..2373
                     /locus_tag="LP524_002829"
     CDS             1..2373
                     /locus_tag="LP524_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235812.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEEIKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGEFEESKKYQATSEEEKAAVRELLEEA
                     VPTDFLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VREIEATPEDKKRIESGQIEVIKKTYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTLEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT          999 a          390 c          545 g          552 t
ORIGIN      
        1 atggcagaag aaaaagaacc actagacaat agtcgtttgg gaaaatccaa aagaaaatta
       61 gtacgattgc aaaatgaatt aaacgaacaa attgaaaaga tgtttgaaca ccagagaaag
      121 accaacggtc aaccaatgaa cgataagaga aatgggcatt cttggtttag gcaacaagag
      181 cgaatcgaaa ataaagtgca tagtctgaga gaagagatca agcaacaaga aaaacaagta
      241 gaaaaacttg aaagacaaga agagataaag gaaatgggct ataacaagta cggcggactg
      301 gatatgacga tcgagaatat tccaagaatc aaagaagaaa tcgaacgctt tgaaaaaggt
      361 gaatcgactt tttcagccgc tacgatccga aaataccaac ggaaattgga aacattagaa
      421 cagctgaaag aaagatcgga aaaaggaaaa gaaaaccttc ttcctgaagt acaagcaatc
      481 attgacagcg gacgagtgac gcaatggaaa aaaaatccga ccatttattt tctgaaaggc
      541 tatcgaaaag tcgctttaga actgaatgaa aacggggaat ttgaggaatc taaaaaatac
      601 caagccacgt cggaagagga aaaagcagct gttcgtgaat tacttgaaga ggctgtacca
      661 actgattttt tgaaggagaa agaaaacgat catttttatc gagtagaatt caacgaaaat
      721 gatccagaaa aaggattgaa aagttatggt ggggagatcg taacattcga attgatcgat
      781 caactcaaaa cgttggataa ccaatacgcc gcagaagcag gttattataa attctatttt
      841 gaagaagtga agaacggaga agtagtcgat cagatccgaa tggatctagg ggatggcgtc
      901 aacagtaatc aacccatcta tttagagtta gcaaaattgt gcgaacaagt acctgagaag
      961 gaacaaaaac aatcggtcaa aaatgaacct gtttcgaaag aacaaatgca gtcgaaagaa
     1021 acaaaaacaa aacaaaaggg ctatcaaaaa cgaacggcag aagagattaa acaagaagtt
     1081 aaatcccttt cgcaacaggc actggatgcg gtgaaaaagc atactcattc tcctgaagac
     1141 gtcaaagaac ttttgaactt tatgtctcgt tttccagaac gatcctttag aaatcaatta
     1201 ttgattgagg aacaatttcc aggtgcaacg gcttgtttag gtcgtgccgc gttgaaaaaa
     1261 gaaggaatct atatcaaaaa aggggaacaa gggaacaaga tctttgtcag aaaaacggtc
     1321 aaaggatttt atgagaaagg acgtggcttt gttcgagaaa tagaagctac accagaagat
     1381 aaaaaacgaa tcgaaagtgg tcaaatcgaa gtgatcaaaa aaacttacta cacgattgaa
     1441 aaagtgtttg atatcacaca aacacagtta aaacctgaag actatccgaa aatctttccg
     1501 aaccgtgtct ttgattttca attggataaa aaaggacagt cagaacttca agctgggatt
     1561 caagcggtag ccaacaatat tggaatacaa attcgagata tgacagaaag tgaagtctat
     1621 caaagagaat taggacaagc acgtggggcg tatgttcgta acgaatttac tggcgaagaa
     1681 gaaatcgtca tgaatactcg aaatactcct acgcagcatt tagctacaag tattcatgaa
     1741 ttagcccatg caagaatgca caaattttca gaattagata ccgcaactaa agaattgcag
     1801 gcagaaatga cttcttatat tgtgtgcaaa cattttggaa tggacacgtt ggagaaagct
     1861 attccttata ttgcagcatg gacgaagaat ggtcagacgt tggatgataa ggaagctgcg
     1921 gaacgtggga aaatcatgaa tgatgtcagc cgtgtcgcaa atgaattcat tcaaaccatt
     1981 tctaatgaaa tcaatcaata cagagaaaga gaaccagaga tccaaccagc agaagtgaag
     2041 caggagcata aaaatactgt agaaacggca gaacaaaaga atattgtgcc tgttggttca
     2101 ctttggatcg acaagaaaga cgggaaagta atggaagaag atacgggtcg aacgttacat
     2161 ttgaaggatg cggcgtttat tgaagatcca cagcataatc aagtaatttt aaaaggaaaa
     2221 agttatggaa aagagatcca aacggcgtta ccttcagaag aatttgcgct aggaaattca
     2281 ttccgatttt cacttgagac agtgtcagaa gtggcaaaac gaaaaaatga aatctattat
     2341 gaacaacaat tgtcacaaaa agaacgtgta taaaacaagt aaaaaagact atctgaaaaa
     2401 cggatagtct tttttctttt gggaggtttt aaaatgaccg agaaaaaaat gagtcttatt
     2461 gatcgatgta aacagatcga tatagt
//
LOCUS       DAFEOM010000148         2459 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.156, whole genome shotgun sequence.
ACCESSION   DAFEOM010000148
VERSION     DAFEOM010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.156"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            5..1150
                     /locus_tag="LP524_002830"
     CDS             5..1150
                     /locus_tag="LP524_002830"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBL2235813.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="LP524_002831"
     CDS             1321..1779
                     /locus_tag="LP524_002831"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBL2235814.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..2408
                     /locus_tag="LP524_002832"
     CDS             1938..2408
                     /locus_tag="LP524_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HBL2235815.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          823 a          460 c          459 g          717 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctttaact taataaaaat aaaaaaagat gaactatttc tagttcatct ttttgcctt
//
LOCUS       DAFEOM010000149         2397 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.157, whole genome shotgun sequence.
ACCESSION   DAFEOM010000149
VERSION     DAFEOM010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.157"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            206..2068
                     /locus_tag="LP524_002833"
     CDS             206..2068
                     /locus_tag="LP524_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL2235816.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2068..>2397
                     /locus_tag="LP524_002834"
     CDS             2068..>2397
                     /locus_tag="LP524_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL2235817.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          864 a          353 c          466 g          714 t
ORIGIN      
        1 aaaatttgaa aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt
       61 taacagtaca tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta
      121 taagttgcac ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat
      181 attagcaatg taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt
      241 gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa
      301 tgttagtgtt cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg
      361 gaagcaagtc ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga
      421 tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca
      481 aagtctccga atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg
      541 aatgaaaatg gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt
      601 gctggaaaat aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt
      661 ttggatcata ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga
      721 gttcacggat tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga
      781 atactttgaa atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat
      841 cattcgaatg cgacacttac tggaatttga aaatctagag aaactgaatt acgcggaatt
      901 actaaatatt ctgattcgag tgacaattgc taccgttcgc ttacgcaaag aggctacgat
      961 tggtcgttat caattggttg ctgaacaaga attagacaaa aacgatcttt cttatcgcgt
     1021 catgcagcag gtctttgatt attatcagtt gccactgttc gaggatgaat atcattatat
     1081 ctatagtgat acattcgaca cgattcacca gcaagacgtc atgaaactag ttgagaatgt
     1141 gatttataaa gtaagtaaag caatcaacca ttctttttat caagatccac aattgttgac
     1201 gaatctgtat gctcatttat ccatgcgatt atcgagaaag aaaaaatttg tcaatgaata
     1261 taatcctttt aaagaagata tcaagcagaa atacccggaa ttatttgatg cgatcaagca
     1321 agcagttcga caagaaataa gtgaacaaaa tattctgatc aatgattctt tcgtggcata
     1381 tattgcctta cactttttgg tttcctacga aagagaagaa gatctacgaa gcgttagagc
     1441 cgtctacatt tgttcaacag ggttgggtgt gactagtctg atcaagcaaa agatcaatga
     1501 agaattaaat aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc aagaaatgat
     1561 tgctaccaaa gatcctgatt tggtgattag tatttttcca attaatggca tcgatcgtcc
     1621 tttcatcaaa gtccatccat tacccactga acaggacttg aaaaaaattc aacaaataat
     1681 aaaaaaaata cttgcttctt ccaatcagca ggaaaggcat aacttgaatc ttagagaaac
     1741 gtccatcaat aaagatactt tagaagactt tagcaaggaa ttgattttga aagcttatac
     1801 gatttatgaa cgtttacttg tcattttgga agcagaattg atccaagaat atcgagaagc
     1861 ttttttgctt catgtattct taatggttca tcgtatcaca tttgatcaac aatatatggc
     1921 agaaggtcgt caagtagacc agattatttt agacaaagga acactggtag gaaaaatcga
     1981 agcagttttt gcagaaaata atttaggaat caatcagtca gaaatcagtg cattattctc
     2041 atatataaaa gaaggagcgt caagttagtg aaaatgtcag acgaggcttt aaaaattatc
     2101 gaggaaagtc aaaatcggga aaagttaaaa caagtgattg atctaattga agagcgatta
     2161 gtagaacatg ctattgtacc aaccgagttg cagtggacca ttctaattaa tcacttgaat
     2221 gagatgctga aacgttctca aagaaaggaa acgattcccg tagtcgatcg ggatttgttc
     2281 aaagaagtat ccaaagaagc cataactatt tctgatgaaa tagttagaag tatcggtaat
     2341 ttaaccgatg atgaaatata tgttttgtcg attcattttg aaacagcaaa aaataat
//
LOCUS       DAFEOM010000150         2164 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.159, whole genome shotgun sequence.
ACCESSION   DAFEOM010000150
VERSION     DAFEOM010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.159"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            <1..111
                     /locus_tag="LP524_002835"
     CDS             <1..111
                     /locus_tag="LP524_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2235818.1"
                     /translation="YAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            325..1020
                     /gene="vanR"
                     /locus_tag="LP524_002836"
     CDS             325..1020
                     /gene="vanR"
                     /locus_tag="LP524_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBL2235819.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            998..2152
                     /locus_tag="LP524_002837"
     CDS             998..2152
                     /locus_tag="LP524_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL2235820.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVPIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          730 a          401 c          461 g          572 t
ORIGIN      
        1 tatgcggtaa agctatataa agaaggaaat atgactgtaa atcaaatttg tgaaattact
       61 aatgtatcta gggcttcatt atacaggaaa ttatcagaag tgaataatta gccattctgt
      121 attccgctaa tgggcaatat ttttaaagaa gaaaaggaaa ctataaaata ttaacagcct
      181 cctagcgatg ccgaaaagcc ctttgataaa aaaagaatca tcatcttaag aaattcttag
      241 tcatttatta tgtaaatgct tataaattcg gccctataat ctgataaatt attaagggca
      301 aacttatgtg aaagggtgat aactatgagc gataaaatac ttattgtgga tgatgaacat
      361 gaaattgccg atttggttga attatactta aaaaacgaga attatacggt tttcaaatac
      421 tataccgcca aagaagcatt ggaatgtata gacaagtctg agattgacct tgccatattg
      481 gacatcatgc ttcccggcac aagcggcctt actatctgtc aaaaaataag ggacaagcac
      541 acctatccga ttatcatgct gaccgggaaa gatacagagg tagataaaat tacagggtta
      601 acaatcggcg cggatgatta tataacgaag ccctttcgcc cactggagtt aattgctcgg
      661 gtaaaggccc agttgcgccg atacaaaaaa ttcagtggag taaaggagca gaacgaaaat
      721 gttatcgtcc actccggcct tgtcattaat gttaacaccc atgagtgtta tctgaacgag
      781 aagcagttat cccttactcc caccgagttt tcaatactgc gaatcctctg tgaaaacaag
      841 gggaatgtgg ttagctccga gctgctattt catgagatat ggggcgacga atatttcagc
      901 aagagcaaca acaccatcac cgtgcatatc cggcatttgc gcgaaaaaat gaacgacacc
      961 attgataatc cgaaatatat aaaaacggta tggggggttg gttataaaat tgaaaaataa
     1021 aaaaaacgac tattccaaac tagaacgaaa actttacatg tatatcgttg caattgttgt
     1081 ggtagcaatt gtattcgtgt tgtatattcg ttcaatgatc cgagggaaac ttggggattg
     1141 gatcttaagt attttggaaa acaaatatga cttaaatcac ctggacgcga tgaaattata
     1201 tcaatattcc atacggaaca atatagatat ctttatttat gtggcgattg tcattagtat
     1261 tcttattcta tgtcgcgtca tgctttcaaa attcgcaaaa tactttgacg agataaatac
     1321 cggcattgat gtacttattc agaacgaaga taaacaaatt gagctttctg cggaaatgga
     1381 tgttatggaa caaaagctca acacattaaa acggactctg gaaaagcgag agcaggatgc
     1441 aaagctggcc gaacaaagaa aaaatgacgt tgttatgtac ttggcgcacg atattaaaac
     1501 gccccttaca tccattatcg gttatttgag cctgcttgac gaggctccag acatgccggt
     1561 agatcaaaag gcaaagtatg tgcatatcac gttggacaaa gcgtatcgac tcgaacagct
     1621 aatcgacgag ttttttgaga ttacacggta taacctacaa acgataacgc taacaaaaac
     1681 gcacatagac ctatactata tgctggtgca gatgaccgat gaattttatc ctcagctttc
     1741 cgcacatgga aaacaggcgg ttattcacgc ccccgaggat ctgaccgtgt ccggcgaccc
     1801 tgataaactc gcgagagtct ttaacaacat tttgaaaaac gccgctgcat acagtgagga
     1861 taacagcatc attgacatta ccgcgggcct ctccggggat gtggtgccaa tcgaattcaa
     1921 gaacactgga agcatcccaa aagataagct agctgccata tttgaaaagt tctataggct
     1981 ggacaatgct cgttcttccg atacgggtgg cgcgggactt ggattggcga ttgcaaaaga
     2041 aattattgtt cagcatggag ggcagattta cgcggaaagc aatgataact atacgacgtt
     2101 tagggtagag cttccagcga tgccagactt ggttgataaa aggaggtcct aagagatgta
     2161 tata
//
LOCUS       DAFEOM010000151         2102 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.160, whole genome shotgun sequence.
ACCESSION   DAFEOM010000151
VERSION     DAFEOM010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.160"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(<1..85)
                     /locus_tag="LP524_002838"
     CDS             complement(<1..85)
                     /locus_tag="LP524_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL2235821.1"
                     /translation="MPRRQRRTYSKEFKQQIVDLYLAGKPCA"
     gene            360..902
                     /locus_tag="LP524_002839"
     CDS             360..902
                     /locus_tag="LP524_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235822.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            1058..1507
                     /locus_tag="LP524_002840"
     CDS             1058..1507
                     /locus_tag="LP524_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBL2235823.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMAPIALDNEHPRI"
     gene            complement(1537..>2102)
                     /locus_tag="LP524_002841"
     CDS             complement(1537..>2102)
                     /locus_tag="LP524_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HBL2235824.1"
                     /translation="ANVLDRTFTQEQPLEAIVTDLTYVRVGKKWHYICLILDLFNREI
                     IGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDRGKEFDNQTIHEILNGFGITRSLS
                     RKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYET
                     PIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          749 a          329 c          399 g          625 t
ORIGIN      
        1 ctgcgcaagg cttaccagcg agatagagat cgacgatttg ttgtttgaat tcttttgaat
       61 aggttcttcg ttgacgtctt ggcatgaaaa atcctccagt gtgtttttta ttattctaca
      121 caccttattt tttctgtcta gtctagtgta gccgattcaa attgatgaag cagatcccaa
      181 agggctgctt catcaatttt ttcgtaagtg tccgtacact tactgtgctt gctcatatat
      241 taatcgatca tatactaatc gtgtaagaac aactttaagc ataatactta tattgttata
      301 ccatagtttg gaaaaataaa aaattatatt tcatcaacgt ttcataaaag gtgtgtagaa
      361 tgttattgaa agtagctata aaatgctatt gtgtaaaaaa taaaggagga ataatttatg
      421 aaaaaatagt accaagagga gcatgcctat tacttgcatt atgtttgagc ttaagcgcag
      481 ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga aggacaatcc ataagttctg
      541 aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga aacttttaag ccatcagatt
      601 atacattgga ggcaaagaaa gaatatgttt atgaatattt agggcttaaa tttaaactct
      661 cagacaaatt tagaaattat atagctgata aaaagatagc gatgttagat gatcaaagtc
      721 ctatagataa agaactaaag tatgctattt tgacatttga gaaaatgaca gaagaacaaa
      781 agaatgcagt tatagaaaag atgggcgatg gatataaaaa ttggcaaaat gagcttgaaa
      841 gaattgggac tattggtata tttgaaaaaa atacatcaga agaaaaaaat ctaaaattat
      901 aaaatgtgat actcatacta aaataggagt ttcaagtgat ggaaaatacg attgctattt
      961 aagcacaaat agcggagctg aaagtaatct tcttgatgaa ttaaaaagaa ctgagattca
     1021 aattatagat aaaaaagaac gtcctaaaaa tggttttgtg ctatcagaaa aaactgattt
     1081 agaaaataca gaagcattta acaaagaatc ggttaaagat ttacgtaaac taccaacaaa
     1141 ggacataaat ggcaaggatt ttacaagtaa agattttgag aagtatgatc ttactatggt
     1201 aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa attcctgatt tggttgaggt
     1261 tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga gttgttacag atcctgttga
     1321 tgataacggt gaaaacaagg aagcaattga aaagtcaaag ttaatacatg aaaagacaaa
     1381 ggcttcatat cctttcttaa tgccagatgg aatggcaaca ctttttttga caaaaattat
     1441 aaggtgttgt gaatgctgcg gtgttatggc tccgattgcc cttgacaatg agcatcctcg
     1501 gatctgattt tctccggcag aagcacaatc attcaattac gctgcctctc cagaggtatc
     1561 agatccgcgc tcattatcaa ggattcgctt cgccaatctc tgttgtcgaa tttggatcgg
     1621 tgtttcatac gccaacgtgc catgtaagcg tagatagttc caccaatgca cataatcaaa
     1681 caattctaga cgtagctgtg ccagtgtctc aaattggtat tgatgcacga attctacttt
     1741 gacagattta taggttgatt ccacaacggc attatcatac ggacaaccct tcctactcaa
     1801 tgaacgagta attccaaaac cattcagaat ctcatgaatg gtttggttat caaattcctt
     1861 tccacggtct gtatgaaaaa gcttgacgtc tgttaaagaa tacggtatcc gtccaaaggc
     1921 ttcttttacc aatgaggcat ctttcttctc accacaggaa taaccaataa tttctcgatt
     1981 aaacaaatca agtattaagc agatataatg ccactttttc cccacgcgaa cataagtaag
     2041 atccgtaacg atggcttcca atggctgttc ttgtgtaaag gtccgatcta atacattcgc
     2101 tg
//
LOCUS       DAFEOM010000152         2097 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.161, whole genome shotgun sequence.
ACCESSION   DAFEOM010000152
VERSION     DAFEOM010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.161"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            554..733
                     /locus_tag="LP524_002842"
     CDS             554..733
                     /locus_tag="LP524_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235825.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            792..1148
                     /locus_tag="LP524_002843"
     CDS             792..1148
                     /locus_tag="LP524_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235826.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT          747 a          283 c          400 g          667 t
ORIGIN      
        1 aacgaaaaaa gccaagactg tttcaagtga atggttagat tattttgaaa atatcaataa
       61 gtatggcgca tattacacca tgaaaaagga agacaacgaa aatgaataag gtgatttatc
      121 aactaacaac ggattccata tcaacgattt gaaagaaaaa aaggtcaata atcatttata
      181 ccgaaaagtg agaatgtgtg aaacaaggac aaaaaaagcc tagtaactaa ggaaatggtt
      241 cttatttcgc accaatcgct ccaaccgccc ccgccgctta ggtggtgcga aataaacggg
      301 caaagcccga taacttaaga gaaagaaggt gatccatttg aacaagatta ttcccttatt
      361 gttagtactg tttgttggcg aaatacttat gcgagatagt gcagctgtac agttaaacct
      421 cgctgtattt ttggtaatac tggtgttttg tttgatcttt tggcggtttg tattagtcct
      481 cgtgagtcta ctattcaaga tgattgcagc catatttttc atagggttat ttcgatgaaa
      541 ggagtgagta ccaatgccaa ttgcaatagg aaataaacgg ttacccgtga cattagatga
      601 aaagagacaa aaagaattgc agcaactaaa gcagaagtat ggcaaaagtg aatctaagat
      661 tatgtgtatt gcgttagatc tattgatcgc gcaagaaaaa gcaggatttg atgtaccagc
      721 gctaaaaaag tgacgtcact ttttaatttg atctatataa gtgatatcac ttttaccaag
      781 gagggaatta aatgttaaaa actaaaaaga attcgtacaa attaaagctt agtatactat
      841 ctattatttg tcttttaggc atgataatta gtggtcattt gtatagagtg gcatatacgc
      901 agtttattga gtacaaggtt cactctttag gattagctgg ggatcgatac gttgaatatg
      961 tccattattc attaaaatgt tcttcatata ttcttctttg gcttattttt tcattgtttg
     1021 aattgctaaa tttaatagca atatcttcat atcacttaga taagctaaat accaaatcta
     1081 gccgttataa cgaagtgttt aaggaaaatg tgaagttaga aagtgactta aacaagctaa
     1141 aaaaataaaa agaagaaaac ataagttaac taaaattatt ttattaagaa tgatggaata
     1201 taaggataag gttaccaaca taacgatcat tacttctctg atatgctatg gacttctatc
     1261 aatcagtatt ttgtctatag attatggagt aactatactg caagccttag ttttggctat
     1321 gcctatgtat ctatctttgt ttatcatgca tgaaatatat aagctatttg ttttatcaaa
     1381 aaatgtagaa atacgggtaa atattttggt gaatagttat caaaaagaac tcaaagaaag
     1441 aagacggtgg cggtatttat tgaaaggatg aaaaagtagg atttgacata ctggtattca
     1501 gaaagtgccg tcacttttaa aacaatggaa aaggtggaga taagatgaag aaaaaacaaa
     1561 ctaaactatc attagcagat atttaacaca gttaaccgca actgaagatg gtgttgttga
     1621 atttgaacaa tctgaaattg cggtagtaga tgatcgttac cttaaaatgc cttacttctt
     1681 tgaccaagca aaggtcattt gcctttgtca ctacgatggg gtacgtgatt ttaggcgtta
     1741 aaaagtagtg attatatcaa ctttacgagt aaatataaat ttagacatga aactgaatta
     1801 tccaatggaa aatcatggtt tataatctag aaaaattata gtaatgattg gattatatat
     1861 attctttcgt attttcgtac aactgtagct tgtcaaaatt gagaaattta accgattagg
     1921 tactttacat cgttatttgg gtaatttcta aaacatgaag cttaccaacg aataaactga
     1981 gactatctta ttaattgatc aatataagaa aatatctttg aggagatgat gtactatgtt
     2041 ttcgtctatc cacgattggt taggtggtct tttcggcggt ggaggtaaag gttttta
//
LOCUS       DAFEOM010000153         2035 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.162, whole genome shotgun sequence.
ACCESSION   DAFEOM010000153
VERSION     DAFEOM010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.162"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            368..1948
                     /locus_tag="LP524_002844"
     CDS             368..1948
                     /locus_tag="LP524_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBL2235827.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLYQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
BASE COUNT          683 a          348 c          434 g          570 t
ORIGIN      
        1 aataaacaag caacacgtct tttcttttag tcagctgaca aaaaaatatt tgactcttat
       61 ggtttggtgg ctttttcatt cgctgaatcc atgctatact gattcagttg tagtagttga
      121 caaaggttgt gaaaaagcta tcctgcatta agtaataaga gcagccaagc aaaaacagct
      181 tttcgtattt ttggcttggg tgggcttatt gctgataggc tgtctctaga acgccgttta
      241 ctcggaaaaa gagactgcta cacggctttt gtcacagccc tatatgaaat tgtctttctt
      301 taagagatcg gacaatctgt gcctagagaa aatataaata attgaatgga agaaatggag
      361 ttaggttatg aataatccaa aattaaagca acaagtggac aatcgcagaa cttttgcgat
      421 catttcccat ccggatgctg ggaaaacaac gattacagaa caattactat tgtttggtgg
      481 ggctatccgt caagccggga ccgtaaaagg caaaaaaaca ggtaattttg ccaaatcaga
      541 ctggatggaa atcgagaaac aacgtgggat ttctgttact agttctgtta tgcagtttga
      601 ttatcaaggg aaacgagtaa atatcttgga tacaccagga catgaagatt tttctgaaga
      661 tacttatcgt actttgatgg ccgtggatag tgcggtcatg gtaatcgata gtgcaaaagg
      721 gatcgaagca cagacaaaga aattatacca agttgtaaaa aaacgtggca tcccgatatt
      781 tacttttatc aacaaactag atcgtgatgg tcgtgaacca ttggaattgt tagaagaatt
      841 agaagagcta ttagatatcg aatcttatcc gatgaattgg ccaatcggta tgggtaaagg
      901 gctagaaggt ctttatgata tctaccataa tcgtgtagag ttttatcgtc cggaaaatta
      961 tcaggatgaa cgattagtag aattggatga agatggactg cttcccgaaa atcatcctct
     1021 aactgaaaat tccttatatg aacaagtctt agaagaagta gaactaatca aagaggctgg
     1081 agatgcattt aatcaagaaa aaattgctcg tggcgatcag acaccagtat tttttggttc
     1141 tgcattgact aacttcggtg tacagacttt tttagagact ttcgttgaat tggcaccagc
     1201 tccttatgga cacaaaacac aagatgatca gatggtaagt ccttatgagg aagaattttc
     1261 aggtttcgta ttcaagatcc aagcaaatat gaatccagcc catcgtgacc gaatcgcttt
     1321 tgtccgaatc tgttctggaa cattcgaacg aggaatggat gtctggttgg aacgtacaaa
     1381 taaaaaactg aaattaagca atgtcacaca atttatggca gattcgagag aaaacgtcga
     1441 aaaagcagta gcaggcgata tcatcggtgt atatgataca ggcaattatc agattgggga
     1501 tacattgttc gaaggaaaac taaaagttgc ttatgaagaa ctcccttcat ttactccaga
     1561 gctgtttatg aaagtgactg caaaaaatgt catgaaacaa aaatccttcc acaaaggaat
     1621 ctatcaatta gttcaagaag gagcgattca gctttataaa acatatctaa cggaagaata
     1681 tatcattgga gctgttggtc agctacaatt cgaagtattc caatatcgta tgttaaatga
     1741 gtataacgcc gaagtaatca tgtctccaat gggacacaaa atcgctcgct ggatcgatcc
     1801 agaagatttg gatgagaaga tgagttcaag tcgcaatatc ttggctcgtg accgtttcga
     1861 ccaaccgttg ttcttattcg agaaccaatt tgcagaacgc tggtttgcgg ataaatatcc
     1921 caatgtaaaa ttaaaaagtt tgatgtaaag aagtcttgac acaagctgcc ctgcatcaag
     1981 taataagaat agccaaacaa aaacagcctc tcatattttg tttagagccg tgacg
//
LOCUS       DAFEOM010000154         2019 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.163, whole genome shotgun sequence.
ACCESSION   DAFEOM010000154
VERSION     DAFEOM010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.163"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            101..571
                     /locus_tag="LP524_002845"
     CDS             101..571
                     /locus_tag="LP524_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HBL2235828.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            793..1896
                     /locus_tag="LP524_002846"
     CDS             793..1896
                     /locus_tag="LP524_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBL2235829.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          687 a          327 c          460 g          545 t
ORIGIN      
        1 agaatctctg aagaataaat cacgtgatcg tgacgataaa atacacttac gtcaggtgat
       61 aaaaaataca taagtagaaa actaagagga ggaacagaaa atgaaagaag ttcaattgat
      121 cagaaaaagc gaactatcag aaggtggttg caatgcatgc ggcgtagtag aagcaacaag
      181 ctatacatta aagcttggtg ccaacaaagc gataatctct gagttgacag taggcggtct
      241 tgtggacagt ctggcgcttg ctgaaggttt tattggagaa gatatttatg agatgttttc
      301 agaaatccgt cagttaaaaa aaggggaaaa ctgcatagag gttcatcatg agagcccgaa
      361 tgtgagattc aaacgtggtg ataatgagat gatcttcaat aatcatgtgt ccgatcacac
      421 cgagctatat ggaatcgtga atcaaatact aactgagctt tttggattgg gaccttatgc
      481 gttcaaggaa gaaaacggta atccaaaatt aaacgaagaa tggcaagaaa cgattgaaat
      541 acaaagaaac aacccgcact tatttcaata ggcatccaca gtatttcgta aaagggactt
      601 tgacacgact tttgtcaaaa tcccatcgtt ttttatctat ttgtgttcta aacatttctt
      661 tcagagtagt aacaactgga atgactgaaa tgtaaacaga ttatttgcct gtatcaatac
      721 ggaagagtaa tataaagtgt gtaaacgcta tcatattatt cattcaattt tttatagagg
      781 gaaggcattg aaatggaaaa aacgtatcaa gcaggtacaa atgaaggaat agtggatttt
      841 atcaatatgg aagatttaga aatagccgct tcacaagtga ttccagctgg tggttatggt
      901 tacatcagca gtggtgctgg cgacttattt acttatcaag aaaatgaacg ggcattcaat
      961 catcagctga ttattcctca tgtattaaga gatgtagaat tacccgatac aactacacat
     1021 tttgacaaag aaacgctgac tgcaccaatc atcatggcac cagttgctgc tcatggctta
     1081 gctcatgtga aggcggagaa agcttctgca aaaggtgtag cagattttgg gacgatctat
     1141 acagcaagtt cttacgcttc ttgtacatta gaagagataa gagaagcagg cggggagaaa
     1201 gcaccgcaat ggtttcaatt ttatatgagc aaagatgatg ggatcaattt ggatattctt
     1261 gaagtggcca agcgtaatgg agcaaaagca atcgtattga cagcggatgc aacagttggc
     1321 ggaaaccgtg aaacagaccg cagaaatggg tttaccttcc cactgccaat gccgatcgtt
     1381 caggcttatc aatcaggagt aggccaaacg atggatgctg tgtacaaatc ttccaaacag
     1441 aagcttagtc caaaagatgt agaatttatt gctgctcatt ctgatttgcc agtctatgtg
     1501 aaaggtgtcc agtcagaaga agatgtctat cgttcattag aatctggtgc tggcggaatc
     1561 tgggtatcta atcatggtgg ccgtcaacta gatggtggtc cagcagcgtt tgattcgttg
     1621 caatatgtgg cagaagcagt agataaacgt gtaccaatcg tgtttgacag cggtgtacga
     1681 cgcggacagc atgttttcaa agcaatcgca tcaggtgcag atcttgttgc tattggcaga
     1741 cccgttattt atggattgtc tttaggagga agtacaggag tccatcaagt gtttgatttc
     1801 tttaaaacag aattggaaat ggtcatgcaa ttagctggaa cacaaacagt agaagatatc
     1861 aaaaaaataa aattgcgtga gaatcgtttt atttaagaaa caaatgacga aaaaaatagg
     1921 agtgcggcag atgatgccgt actcctattt ttatagttca aggatgtcac taatagaata
     1981 atttgaaacg aaaaaataaa attgaaatat tttttcata
//
LOCUS       DAFEOM010000155         2001 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.164, whole genome shotgun sequence.
ACCESSION   DAFEOM010000155
VERSION     DAFEOM010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.164"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            <1..376
                     /locus_tag="LP524_002847"
     CDS             <1..376
                     /locus_tag="LP524_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBL2235830.1"
                     /translation="NVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFRR
                     FPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTDD
                     WQKVMAAEVIATYLDGDLVYKK"
     gene            424..1824
                     /gene="yfcC"
                     /locus_tag="LP524_002848"
     CDS             424..1824
                     /gene="yfcC"
                     /locus_tag="LP524_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBL2235831.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1857..>2001
                     /locus_tag="LP524_002849"
     CDS             1857..>2001
                     /locus_tag="LP524_002849"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235832.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          553 a          339 c          432 g          677 t
ORIGIN      
        1 aaatgtttcc acagaggact tctttaatta ttgtcgatta gaggaaagtg gtgtaacagt
       61 cactagcggt acagatttac cgctgtttat tactgatatt cctgaatcga ttataagagc
      121 agtattccgt cgttttcctg aaggagaaga agtctgggag aaagaaacaa gcttcacgac
      181 aaccgagctg ctgaaaagtt ttacaagcaa cagcttcaaa gtaaatggtt tatcggagta
      241 cggagaaata aaagtaggga aaagcgccag cttagctgtt ttcagcgagg acctctgtac
      301 tgatgactgg caaaaagtga tggctgcgga agttatcgct acctatcttg atggtgactt
      361 agtttacaag aaataatgct tttcgatata acgttataaa acgaattaaa gagaggttaa
      421 aaaatggaaa caatgaaaag aaaattcacc ttcccacata gttatacatt gatttttatc
      481 ttaattattt tggctgcaat tttaacttgg ataattccta gtggtcagtt tgagacagtt
      541 gttgaaaacg tcgcaggtat ggaaaaaaca acggttgttc cgggaaccta tcaccaaatc
      601 gataaggcag attatggaca aggtttttcc tctatactaa tggcaccagc tgaagggatt
      661 atttcagctg ttgaggttgt tgcttttgtt ctcatcgttg gtggtgcctt cgggattatc
      721 ctaaaaacgg gagccatcga tcgagggctg tttaccttag caaaagttct gggagacaag
      781 gggatcttag ttattccgat gtcgatgatt ctattcagct taggaggatc aacctttggc
      841 atgagcgagg aagtcattcc cttatttgcg gtgtttattt ctctgatgtt ttctcttggc
      901 tatgattcca tgactgcgat cttgattcta tttttaagta cacaagcggg atatgttggg
      961 tcgacgatta atcccttcaa cgttttaatc gcacagggag tagctggcgt ttacggaaat
     1021 cccctgcttt ggtttcggat gctatcctgg attgtcatta ctgggatttc aattgttttc
     1081 accatgcttt atgctagcag agtcaaaaag aatccactgt cctctattgt ttatgagagt
     1141 gatttaaagg ttaagcataa gtttcaaaat tttgacactg aaaaagagtt ttcaacacaa
     1201 gataaactaa ttatcgccgg ttttatcgta ggtttaggaa tcatgatttg gggtatcatt
     1261 tccaagggat ggtatatggt tgaaatttct gcactattct taggattagg tctttttgct
     1321 gggggcatcg ctagattttc tcaaaaggaa attgctgaag cttttgtcga aggctgttca
     1381 gatttcgcct atgctgctgt aatcattggg ctagctagag gagtcttggt aatcttggaa
     1441 ggtggaatga ttattgacac tattctaaac agcttggcta accttttagg tggtcttcca
     1501 aaattcctgt tcactacgat tctactcatt tctcaagtaa ttgtttcctt ttttgttcca
     1561 tcctcctcag gagctgcggc attaacgatg ccggttatgg caccattggg tgatctaatg
     1621 aatgtaagtc gtgatgttgt tgttctaagt aaccagtttg gaaatggttt aatgaatatt
     1681 atctccccaa ctagtggtgt attactggca ggattagcca tcgcagagat taatttctct
     1741 aaatggttta aggttggctt caaaatattt gccatcttaa cccttgtttc tgctgttttg
     1801 ttatacattg ctacactctt ttagtcttaa tcatccatta ataggaggca atagttatga
     1861 ttaaaataaa tcaattagca ggaatgaata tccactattt attttattct ctcaaagaat
     1921 ttttcgctta tcaacagaaa attggtattt cttctgttga actttgggga ggtgctccgc
     1981 attttttcat agatgctttt a
//
LOCUS       DAFEOM010000156         1981 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.165, whole genome shotgun sequence.
ACCESSION   DAFEOM010000156
VERSION     DAFEOM010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.165"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(<1..196)
                     /locus_tag="LP524_002850"
     rRNA            complement(<1..196)
                     /locus_tag="LP524_002850"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(869..1225)
                     /locus_tag="LP524_002851"
     CDS             complement(869..1225)
                     /locus_tag="LP524_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HBL2235833.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(1239..1649)
                     /gene="spx"
                     /locus_tag="LP524_002852"
     CDS             complement(1239..1649)
                     /gene="spx"
                     /locus_tag="LP524_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBL2235834.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
BASE COUNT          591 a          379 c          299 g          712 t
ORIGIN      
        1 gattgttata cggtattagc acctgtttcc aagtgttatc cccttctgat gggcaggtta
       61 cccacgtgtt actcacccgt tcgccactcc tctttttccg gtggagcaag ctccggtgga
      121 aaaagaagcg ttcgacttgc atgtattagg cacgccgcca gcgttcgtcc tgagccagga
      181 tcaaactctc ataaaaagtt cgaacaatct tgcgattgtt aagctcaatt tgtttgctag
      241 catattgctt gcttgttaaa aaatgtttgt tgtcttgaat tgaatcaaga cgccctacac
      301 atttggtttg tcttactttg ttcagttttc aaaggtctac ttcatcagga gtgttccccg
      361 attcgttttt ggctgccgta gcaactttta tatcatatca gagagacaag taattgtcaa
      421 ctctttttta aaagtttttt tgatttcgtt tttcgaaatc ttacagcaac tcatttatca
      481 taacttatca tttgttattt gtcaaggact tttttgaagt ttttttcgat agaatcaagc
      541 ttcacaagtg gttctttttt aacaactcag ttatcttatc atgtcatttc taacatgtca
      601 acaactattt tatatgtttt ttaaaatact attttattgc tttgctactt aaaacaacag
      661 aaatgaatat atcatgtaaa tttacacatg tcaatatatt tttaactcta tatccccacc
      721 tagatttgaa ttttgaccac ataaattgag catgtctcta tagtagaaaa ataaagtcaa
      781 aaaaagaaca cataacacgc ttgatgctaa gtgttcgact gttacaactg tatgaaatgc
      841 tcagtgactt gattaaaaca gttaaagtct atttatttaa gataagctgt tccatcaact
      901 gctcattgta tccaataaat agaacttcat cgataataat caaaggacgt ctgaggagat
      961 tggtatgttg tgtgcataaa aaaatcagtt gtttttttcc tacacaggaa aggtcaatcg
     1021 caatgttgtg aaaagcctca gaccaagtcg caaatagctg ttctgcttgc acttcttcaa
     1081 aactgcataa ttcttctgct tcttctgtac ttagatcgcg ttcttctaag tcaacaaatt
     1141 gataaggcaa ttgatggtgt tctaaccatt gtttcgcctt cagacttgtt acacagtgcg
     1201 ctgatccata gactttcact tccacgaagg ctccctccct atgaatttaa gatgttattg
     1261 ttttgtaaat agatcagatt tctggcaaca catcgaattt cacgagggac aaatgaccgg
     1321 atctcatctt cattaaatcc gattagtatt cttttttcat caatcaaaat aggtcgtctc
     1381 aataatgaag gatattcttt gaataagacg atcatttgct ttaaagttaa ttcgttaaaa
     1441 tctatgccca gcttttcgta aattttcgac cgagtggaaa taatatcttg aactcctcct
     1501 tcagtcgctg ccaagatctt tttgatctca ctttctgtta acggttcggc aaaaatattc
     1561 ttctcttcaa attcaagttg attcgcttgc aaccatgctc ttgcttttct acaggaagta
     1621 cagctaggcg aagtatatac ttttatcatt ttctcactcc tttttaatta taatccaatt
     1681 ctacagtaat ttcttttaaa ataccaatta ttagctaata aaatataaaa aaaatcaaaa
     1741 taactgatta tttacgtttg aataatgaga atttttctat atattctcta ttttgataat
     1801 agtattctca ttttcaaaat aaataaggga ggtgtgacaa aattcttgtc acacctccct
     1861 tttccgaata aactgtgttc cagaaatagc ttcttcgaaa ataagctgaa atttcacaaa
     1921 aatttgagaa gcaattttcg tacattcctt cttctttctt gaaactgacc acttctgtca
     1981 c
//
LOCUS       DAFEOM010000157         1980 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.166, whole genome shotgun sequence.
ACCESSION   DAFEOM010000157
VERSION     DAFEOM010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.166"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(325..529)
                     /locus_tag="LP524_002853"
                     /pseudo
     CDS             complement(325..529)
                     /locus_tag="LP524_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(729..1745)
                     /locus_tag="LP524_002854"
     CDS             complement(729..1745)
                     /locus_tag="LP524_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBL2235835.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          558 a          459 c          309 g          654 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaaaa attttcctaa ttgtgacatg gctgtcacaa
      541 ttccaaatgc tctccgaggg tattcgctgt cacaagtgac aacgaaaaaa ggagccgaaa
      601 gcgtgtcacg ctctcaactc cttttacgct ttattttgct tcatgcaaaa tattcgctta
      661 tcgtgaattt acgtcactaa acattgacgc aaatccactc tcactcatct gacttttctt
      721 attcggtatc acttctctgt cacatccaac cccaaaagcc acgtatccaa ttcttttttt
      781 aattcgtctg cagattcctt aaacgtctct cgtaaatgat tcgttaactc atcattttga
      841 ctttcttgct tcagcaaatt ccgatattta tattttgtat gtctagcaat tatcgaccaa
      901 ttagaaggct cagctatcaa accatcaata actattttat caataggttt tatatcaatc
      961 ggcaaaaaat gcgggcttgc aaaactatct aagctttcct gaaccatcgc atgccaatcc
     1021 gttgctttgc cacgtcttag ctgcatttct aaccgccacc acgtatcgat ttcttttggc
     1081 acgattactt tttttcgttc ctgctctagc tttttgttgt acagtcgcac ttggcgctca
     1141 ctggcacgac tgccccagta cgctgtttct agcttgccag aacggccgta gattggctta
     1201 aaagaaatcg ggtctacaat acgatactga gtaataaagt catccggcac atctacaata
     1261 tcgcaggcaa tatccgcccg ggaaaaatgc ggatctaaaa ataggtcatg aatgaatcct
     1321 ttcatggaac tagctaaaaa ctggcctatc ttattcggat taaaatcaat acgacctttt
     1381 ccttcatgaa atttaagtat ttcgatatac gcaatatctt ctccatattg atcgaataat
     1441 tgccagccgt ttatcccaac tgatttcaaa tatccctgat tttcgttcaa acgcattaat
     1501 tgctcgaaat cgacaaaaac aactccattt ttcgtccaat aagctatggt atcattcagt
     1561 tttccgacaa ttgtaattcg atcaatcgac caatttaatt ttaggggggt ggtcgtacct
     1621 tgcacactta accgcctgtt agaaatacgg ttaagttttt cgagctgatt tatcgttgtt
     1681 cttttttctg cccctcgacc ttcaattttt gattgaattt cactcaactg attttcattt
     1741 gccattttcg tcctccaaac gaaaaaagag acttttggaa agaatttcca gagttcacgt
     1801 aaccctaaaa attcatatcc aaaaatctct agcttgaaga aacctaaaaa tattcgtata
     1861 atgaacctga gttacggagc taggtgaagt agtttccgta aatagtcgag agtgttagcg
     1921 cactgctcgg cttttatttt gctctttttt ctcgttttct ttgattatag cactcctcct
//
LOCUS       DAFEOM010000158         1972 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.167, whole genome shotgun sequence.
ACCESSION   DAFEOM010000158
VERSION     DAFEOM010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.167"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(62..241)
                     /gene="rpmF"
                     /locus_tag="LP524_002855"
     CDS             complement(62..241)
                     /gene="rpmF"
                     /locus_tag="LP524_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBL2235836.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(299..868)
                     /locus_tag="LP524_002856"
     CDS             complement(299..868)
                     /locus_tag="LP524_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBL2235837.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1054..1956
                     /locus_tag="LP524_002857"
     CDS             1054..1956
                     /locus_tag="LP524_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL2235838.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
BASE COUNT          587 a          419 c          332 g          634 t
ORIGIN      
        1 aaaataaacc tcaaggctcc atggttagcc ttgaggtttt acattttcta attaaaatgt
       61 attaagcttc ttttgacatt acgtccttac catcatagta accacaattt gcacatacgt
      121 gatggctttt tttcatttca ccacagtttg gacattcgtt taaccctttg attgtcaatt
      181 tgtagtgtgt acgacgtttt gcttttttag cttttgatgt tcttctagct ggtactgcca
      241 ttttgctaca cctccttgta aagtcttgta tctaaattag acacaatata ttccaatctt
      301 acttgttatc gtcgtctttt gatttagaat caaaaagttc agatagtttt gcaagacgag
      361 gatcaatttt attctcggct tcttccattt ttctgcgctg gtattcttct tcggaaacaa
      421 cttcccaatc attgcctttt ggaagttctg ttgctgcttc ctcttctttt gtcaataccc
      481 gcattggaat cgccaataag atgttgtctt caatcgactc tgctaaattc aataatggac
      541 cttcaattag caaaatctca tctgcagaca tttgttcatc tctactttgg aattgttctt
      601 ctgtcatgaa tacttcgtct acagaaaaat ccatgggcag ttcaacaggt tctagtgaac
      661 gtgttgaagg aactgtcaaa gtcgtctgca cagtgtaatg aacgatatag cctttcttat
      721 caactgagac caaaccttcg acttccactg gcgcaacatc aagtattaaa ttgtctcgct
      781 tcatgagagc ggctttcaag tcgagcgtct catgaaaagt taacggtgtc tcctggtatt
      841 ttctcaattc caataaagac catttcattt ttcatcactc ctaagcaaca aaagcaatta
      901 tacagggcaa aaagcatgtt gtcaatgaaa ttttcttgac accttgcagt tcataaaaaa
      961 acagaaaaat ctttattaga aagcctctgt ttgctatcat cctcagaatc atttacaata
     1021 atcattattc cattaaagga gcggatgatc ttcatgaaca cgaaagatat cgaatactat
     1081 gtccaaatcg tcaaacaaaa aaattttacg aaagtggcta attatttccg tgtgagccaa
     1141 cctactatta catatgcatt acaacgtctt gaaaaagagt ttgacaccgt acttattaca
     1201 agagatcgat cgcatcacga attaatcgtc acgccttctg gaaagcagct gtttctccat
     1261 gcacaaacca ttctccaaga gatttctact gcaagagctg aaatcactca gttgcaaaag
     1321 aaaaagcttc gttttggcat gcctcctatt atcggcaatg aatactttcc taaactgtca
     1381 agttacttta tcaagcatga attgatgaat caaattgaga ttaccgatgg aggctctcgg
     1441 gacttatacg ggatgatccg acaagggagg atcgatttag ctgtattagg ctcaacacag
     1501 ccaatcgtgg atcaatattt agaaacagag ttacttttgg acaaacgttt catgatcgtc
     1561 gtgccaccta cacatcctct ttccaagcag gatactgtct ctttcaaaga tatagcagat
     1621 gaacagtttg tactattgaa cgaacattat gtccaccccg cagcattcac taagctttct
     1681 aaacaaacac atactcatcc aaaagttgtt tatcaaagca atgacttgac gattttaaag
     1741 agtatggtcc gagaaaatgt gggtatcggt tttttggcag agattgctgt ccatcctgaa
     1801 gatcatttag tttgcctccc tctttcagat gaagttcagc ctcgcttctt gatttcattg
     1861 gcaaaacgaa cacagcagat cgtcacgcct cttcaagaac aagtagtcca agttatccat
     1921 gaattcgtta cagcacaaaa aaacagctct tcgtaaaaga agagctgttt tt
//
LOCUS       DAFEOM010000159         1939 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.168, whole genome shotgun sequence.
ACCESSION   DAFEOM010000159
VERSION     DAFEOM010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.168"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            <1..367
                     /locus_tag="LP524_002858"
     CDS             <1..367
                     /locus_tag="LP524_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBL2235839.1"
                     /translation="KKQIVPKEIETWWRIEMQLRRGKATDWHAMVRESLDSFASPHYL
                     PGDVKPVDRIMIKGLNQDHSEWAYISRNLKYRLRKLLKEESQNDELTNHLRETFKESA
                     NDLKIELDTWLLGLDVTEK"
     gene            567..770
                     /locus_tag="LP524_002859"
     CDS             567..770
                     /locus_tag="LP524_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235840.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSAKEGFNLGFSISFKKGTKN
                     TSISHNNRELTDSQKINSLSGRK"
     gene            1341..>1939
                     /locus_tag="LP524_002860"
     CDS             1341..>1939
                     /locus_tag="LP524_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBL2235841.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLSVDDKVHPLKLSWSIDRITIVG
                     KLNDTIAYWTQNGVVFVDFEQLMRLNENQGYLKSVGTNGWQLFDQYGEDIAYIEMLKF
                     HPDKGRIDFNPNKIGEFLKGSMKQFIHDLFLDAHFSRADIACDILGVPDDFITQYRIV
                     DPVSFKPIYGRSGKLETAYWGSRASERQVRMYNKKLEQER"
BASE COUNT          630 a          292 c          457 g          560 t
ORIGIN      
        1 aaaaaaacag attgtaccaa aagaaatcga aacatggtgg cgtatagaaa tgcagctaag
       61 acgtggcaaa gcaacagatt ggcatgcgat ggttcgggaa agtttagata gctttgcaag
      121 tcctcattac ttgcctggag atgtaaaacc tgttgataga attatgataa aagggctgaa
      181 tcaagatcat tcagagtggg cgtatattag cagaaatttg aaatatagat tgcgcaaatt
      241 gttgaaagaa gaaagccaaa acgatgagtt gacgaatcat ttacgagaga cgtttaagga
      301 atctgcaaac gacttgaaaa tagaattaga cacgtggctt ttgggtttgg acgtgacaga
      361 gaagtgatac cgaataagaa aagtcagatg agtgagagta aatttacgtc aatgtttaat
      421 gacgtgagtt tacgataagc gattattttg cgtgaagcaa aataaagcgt aaaaggagtt
      481 gagagcgtga cacgctttcg gctccttttt tcgttgtcac ttgtgacagc gaataccctc
      541 ggagagcatt tggaattgtg acagccatgt cacaattagg aaaatgttac tctttgtcaa
      601 agagtaacag aaaggtttac actcttttta ttagcgacag cgcgaaggag gggtttaact
      661 tggggtttag tatttcgttc aaaaagggaa caaaaaatac aagcatttct cataataatc
      721 gtgagttaac tgatagccaa aaaatcaata gtttgtctgg tagaaaataa ttagcagatt
      781 tacaagtggg aaataaaaag ctgaaagaag aaaattgcat ttcaaactat attttctaca
      841 atagctctaa aatggcatat tttgcgttct gagcggtttt agcgtttatt tcgtttagtt
      901 atcggcacaa tcggtgaaac acgttagagg agcgaaaatg gagcctgtaa ggcgactgta
      961 aggcgactgg tagcggaacg gtcgtgttag attgtgtagc cgatagctga acgaaataaa
     1021 tgctggcagc gccattctat ttcggcagga ggaggctcaa ggggttcggg gaatgaaatt
     1081 ccctgaggtc tttttatttt ttctagagga gtgctataat caaagaaaat gagaaaaaag
     1141 agtaaaaaaa tagtcgagca gtgttgacgc actctcgacc tttacggaaa ctacttcacc
     1201 tagttccgta actcaagttc attatacgaa tatttttagg tttcttcaag ctagagattt
     1261 ttggatgtgg atttttagag ttacgtgaac tctggaaatt ctttccaaga atctcttttt
     1321 tcgttgagga ggagctattt ttgagtaaaa aaggcataat cgtaaatgag tgggaaaaac
     1381 ttaaccgtat ttctaacagg cggttaagtg ttgacgataa ggtacacccc ctaaaattaa
     1441 gttggtcaat cgatcgaatt acaatcgtcg gaaaactgaa tgataccata gcttattgga
     1501 cgcaaaatgg agttgtgttt gtcgattttg agcaattaat gcgtttgaat gaaaatcaag
     1561 ggtatttgaa gtcggttggt acaaatggtt ggcaattatt tgaccaatat ggagaagata
     1621 tcgcctatat cgaaatgtta aaatttcacc ctgacaaagg tcgtattgat tttaatccaa
     1681 ataaaattgg cgagttttta aaggggtcga tgaaacaatt cattcatgat ttgtttctgg
     1741 atgcgcattt ctcacgtgca gatattgctt gcgatattct cggtgttcct gatgatttta
     1801 ttacccagta tcgaatcgtt gaccctgtgt cttttaagcc aatttatggc cgttctggca
     1861 aattagaaac agcgtactgg ggtagtcgtg ccagcgagcg acaagtgcgg atgtacaaca
     1921 aaaagcttga acaggaacg
//
LOCUS       DAFEOM010000160         1927 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.169, whole genome shotgun sequence.
ACCESSION   DAFEOM010000160
VERSION     DAFEOM010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.169"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            754..1839
                     /gene="xerS"
                     /locus_tag="LP524_002861"
     CDS             754..1839
                     /gene="xerS"
                     /locus_tag="LP524_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HBL2235842.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          719 a          325 c          264 g          619 t
ORIGIN      
        1 acagtaagcc tacccgttaa tctgaataga acgtaaaaga aaaccactag cccgccagct
       61 agtggttttt ggtgtatcat gaattacctt ttcgttaaaa caattatacc atatttccta
      121 aaaaatacaa ttaatagacg aattatcatt accactttga tgaaaaaagc tgacataaat
      181 ttttctttta tttttataat gaataaacat ttatcaataa gcttttgaaa ggcaggatcg
      241 tactttgttg atgattgaga tattgctaca tcaatctcag cagttaagat ttttttgaat
      301 tgttgcttga attttttctt tatctaaaaa aattgtatct ccaggatgca acccattttc
      361 tttaagaaat tccttttaaa ttgttaatac tactgagtgc cccacttctc tgaattttct
      421 ttaacttctc cctccccttt tttatttgta tatacattca tatacgcttg ttaatatcaa
      481 atcaaccgta gtctctctta tgtctaatta tatagaaaga ttgttctttc tatataattt
      541 agacaattta aaaaattaag attgcctcct tctgtttcct ttcctattca tgtataccat
      601 ttattttaaa ttattaaatc tatacctatt tatctgcttt tttgaaataa attaataaat
      661 ttcagagtat accaattgac aaaacactct ataattggta tagtccaatt atagagatac
      721 tcctctcccc tacttatcga aaggatgaat aacatgaaaa atacagaaat atatcaaaaa
      781 atagatacaa tcatacttca tatgcccgat tatataaaag attttgttca agatcgtgag
      841 gataaagatc aatcacctcg aactacttta gaatatctaa aaaattataa gctcttttat
      901 gaatggcttc tgagtgagtc tatcgttccc gatacttcaa tcacctcgat aagacagtta
      961 actgcctatg atctaaactt gtacaaatct catttgaaac gtcgcacaaa ggaaaatgta
     1021 aaaaaagaga caacaaaaac taagctcaaa gataataata acttaggtct atctacttct
     1081 actatcaata gaaatatcac agcattaaag gttctattta aatatctttc gaaaagttcc
     1141 aataatcctt taggaaaacc atacttagaa aataatccta tggatcaagt agcaactatc
     1201 acagataaaa ctacgttagc tgcccgtgca aacgcaatcg agaaaaaatt atttctagac
     1261 gaagatacac aaaattatct agattatatt gccaatgaat ataaaaaaac tttgagcaaa
     1321 cgagctttga tctattatca acgagatgta gaaagagacc tggcgatcaa tgcattgatt
     1381 ttaggatctg gattaagact ctccgaggtt gtaaatatta atttagagga cctatcttta
     1441 gaaaagaata atgtggtagt aactagaaaa ggaaataaga gagacgcagt aaatatagca
     1501 gcatttgcaa tggaatattt agctaattat ctagctattc ggaaagaacg atacaaagta
     1561 acagaaaacg aaaaagcttt atttctagca atttatcaag gagaagccaa gcgtatatcc
     1621 ggtattgcaa tcgaacggat ggtagcaaaa tattcaaaag gattcagagt ccaagtcagt
     1681 ccacataaat tacgtcatac tttggctact agactctatc aacagacaaa ttcattggta
     1741 ttaactgccc aacaattggg acatacaagt acaaatacaa caactttata tactcatata
     1801 gacaatgcag ctacaattga tgctttgaac gctttgtaaa tgtagtaaaa aatagtataa
     1861 taatgaagcc tgtctctaaa attagataaa agcccaactt taaaggcaag cttcattatc
     1921 attttta
//
LOCUS       DAFEOM010000161         1762 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.170, whole genome shotgun sequence.
ACCESSION   DAFEOM010000161
VERSION     DAFEOM010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.170"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            <1..400
                     /locus_tag="LP524_002862"
     CDS             <1..400
                     /locus_tag="LP524_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL2235843.1"
                     /translation="EALKRHYHRLFKRPISQTEIVNELLSYNEELRTAYHFCQLLRYY
                     FVKRDTVGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNGVTEGLYNKI
                     KLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            579..>1762
                     /locus_tag="LP524_002863"
     CDS             579..>1762
                     /locus_tag="LP524_002863"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBL2235844.1"
                     /translation="MSMNHFIKQLFTIKDSNIQIQSFEETFYKSRKSIVIEGTLIRTY
                     RRCPCCKDSTKQIVKNGKKISMILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLV
                     TPFCFISKQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPR
                     CLLFDEFRSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHY
                     IVTDMNAPYFSLVKECFPNAQVIVDRFHIVQHLNRFFDAVRKRVMKTLDQKDPIQAKQ
                     YRQLKSLFKLLLKSEDRLDYNTLTKRRNFNWRLLTETEVVDRLLKISPELKTAYRYYQ
                     DLLTAYQEKDPDTFFQLLRAMPGEVPLELHHIKKAFFKYEKGIRLALLKKYSNARLEN
                     LHT"
BASE COUNT          555 a          356 c          320 g          531 t
ORIGIN      
        1 ggaggcactc aaacggcatt atcatcgact gtttaaacga ccaattagtc aaacagaaat
       61 cgttaatgag ctgttaagct acaacgaaga attgcgaact gcgtatcatt tttgtcaatt
      121 attacgctat tatttcgtta aacgagatac ggtaggcttt caagaaaccc ttaaaacaat
      181 ttcatctacg ttacctcagt cgtttaaaaa gaaattcacg atcttccatc gctaccaatc
      241 gggtatttca aatgccttta aggtgtccca ctcaaacggc gtcactgagg ggctgtataa
      301 caagatcaag ctgattaaac gaattgcgtt tggttatcgc aacttttata actttcgggc
      361 acggatctat ttacaacaag gtctgatctt tgaaaactaa aaaaatatgc ggcgcacaga
      421 acgcgccgca tatcggacta agttttattg gctctataat ttctaggact gatggaaatt
      481 ttctatctgt cctagttttt ttgtaaaaaa acaaaaagac atacaaaatt tccggtagaa
      541 tagagtctcc tacaacatca ttctggggga attcttgtat gtccatgaat cattttatca
      601 aacaactctt tactattaaa gactcaaata tccaaattca atcctttgaa gaaacttttt
      661 ataaaagcag aaaaagtatc gttattgagg gaactctgat ccgtacctac agacgttgtc
      721 cgtgttgtaa agattctaca aagcaaatcg ttaaaaatgg caagaagatc tctatgattc
      781 tcctgaatcg tagcggtaat aaacggacct atcttcggtt gaagaaacag cgatacctct
      841 gccgagcatg taaaaaatat tttaccgctc ggacgtatct cgttacccca ttctgcttta
      901 tttctaagca gattcattac aagatcttag aagaattgac agagcgtcaa tctataaaag
      961 caattggtaa acattgcgat gtttctgtga caactgttca gcggacattg tccgccttgt
     1021 catcagatat ccgtcagaat cttgagtatt tacctcggtg tctattattc gatgagtttc
     1081 gttctctttc tacatggcac ggtaaatttt ctttttcttg tatggacggt gagaccggga
     1141 aactgtttga gatcttacct agccgccgta aaaaagatct cgttgctttt ttcatgcgtt
     1201 tttctctcaa agcgaggttg ggtgtacatt acatcgtaac ggatatgaac gctccttatt
     1261 tctcactagt taaagagtgt tttccaaatg cacaagtgat cgtcgatcgg tttcacattg
     1321 ttcaacatct gaaccgcttc ttcgatgcgg ttcgaaaacg tgtgatgaaa acacttgatc
     1381 aaaaagaccc aatacaagct aaacagtatc gccaattgaa atccttattc aaattacttt
     1441 taaaatcaga ggatcgttta gattacaata cactaacgaa aagacgcaat ttcaattgga
     1501 gactattgac tgaaacagaa gtagttgacc gccttctaaa gatttctcct gaacttaaaa
     1561 cagcttatcg ttactaccaa gacctgctta ctgcgtatca ggaaaaagat ccagatactt
     1621 ttttccagct gcttcgagca atgcctggag aagttccatt ggaactgcac catatcaaaa
     1681 aagccttttt caaatacgag aaagggattc ggctggcatt actgaagaag tattcgaatg
     1741 cacgactgga aaatctccat ac
//
LOCUS       DAFEOM010000162         1752 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.171, whole genome shotgun sequence.
ACCESSION   DAFEOM010000162
VERSION     DAFEOM010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.171"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(192..569)
                     /locus_tag="LP524_002864"
     CDS             complement(192..569)
                     /locus_tag="LP524_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235845.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(869..1750)
                     /gene="galU"
                     /locus_tag="LP524_002865"
     CDS             complement(869..1750)
                     /gene="galU"
                     /locus_tag="LP524_002865"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBL2235846.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          503 a          381 c          254 g          614 t
ORIGIN      
        1 aaacattggt atgatttata tatatataaa taacataaaa aaaggaatgg cttatcccga
       61 tacaacaagc attcctttaa aaaagattct tttatataag tgaaaaaagc ttttttccac
      121 caattaaata aatctctcat ctcaatagtt tatccattat tttatctttt tttgcgtata
      181 cctactaaat attaccgtct tcttctcgtt cttctacttt gttcgagaga atctgtacta
      241 gaaaaagtag ttgtatgtct accttttctg cctatccgag actttctttt catatatggg
      301 atataaatga ttagatacag tacagcaaaa aataaagcaa atacgcaagc ttcccaacta
      361 cctaacgcca attcaaccat caaggtagca accccaacgg atgctgcata ccagataaaa
      421 atcagaaacg tttctttttt tctgaccatg cgaaacatga taaatagact aataagcaat
      481 agtggcaaca caaatatcca attgcttcct tccatcagcc acttgagtat atcaagcagc
      541 gactgtgcaa cagcgactaa ttttcccata gtgaacacac accttttttt ataattaaaa
      601 aattcgcctt tttattctac tatgaatctt ctggcttggc tttcatttct cttatgtaat
      661 cattttcttt tatacaactt aaaaaaaaat taaaccatta gcataatatt ttctgaatct
      721 attcacgaaa gtctttttct atctttcgtt ttccacgact caactgatct agagtcaaaa
      781 aactaaaacg acagttgcgc aaatgacgca caagctgccg ctttaataac aaatagcctt
      841 tcattctcag caggccactt atttccagtt aactttctgt tcttagcttt ttactcaact
      901 ggataatata tgcacttaat gaagtcccaa tttccgaatg ttgaagtcca tattcaatat
      961 ttgtcttcat atatcctaac ttatcgccta cgtcgtatct ttttccttta aactctctcg
     1021 catagaccgc ctctgtctgg ttaagagtct gaagtgcatc cgttagctgt atctcgtttc
     1081 ctgctcctgg ttcttgagtt tctaatatct caaatatttt aggagtcagt agataacgtc
     1141 cgataatggc taaattactt ggtgcttttc ccatttcagg tttttctaca aaccctttta
     1201 ctctgcaacg acccttttcg ctatctgttt taggatcaat aattccgtat ttagatgttt
     1261 cattttctgg tacacgcatg acggcaacag cggaagcttg tgtttcttca taatccatca
     1321 tcaattgttg agtcaatggg atatcatctt tcatcaaatc atcccccagc atgacgacaa
     1381 acggttcatt tcctataaat gcttttgctt gaagcacggc atcccccagt cctcttggat
     1441 gagattgacg gatgaagtga agattgactt gagtcgtttc ttcaactaaa gcgagtaatt
     1501 ctgttttttc cttttcccgc aaattgtttt ccaattcgat atttgcatca aagtgatctt
     1561 caatgggtct tttttctttt cccgttacga tcaagatgtc ttcgattccc gaatccaatg
     1621 cttcttccac aataaactgg atcgttggtt tatccacgat tggaagcatt tcttttgcca
     1681 tggcttttgt tgccggcaaa aaacgtgttc ctagcccagc ggcaggaatg actgcttttt
     1741 tgaccttcac tt
//
LOCUS       DAFEOM010000163         1698 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.172, whole genome shotgun sequence.
ACCESSION   DAFEOM010000163
VERSION     DAFEOM010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.172"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            <1..903
                     /locus_tag="LP524_002866"
     CDS             <1..903
                     /locus_tag="LP524_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBL2235847.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGF
                     KACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESL
                     IGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGW
                     KVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELG
                     MICGKLDRQYYSASAISSIVIPPK"
     gene            916..>1698
                     /locus_tag="LP524_002867"
     CDS             916..>1698
                     /locus_tag="LP524_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HBL2235848.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFS"
BASE COUNT          564 a          248 c          389 g          497 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga catttacaaa tggacaatgg atgggcgtag tacaacctac gacggttgat
      301 ggatttaagg cctgtactga tccaagacaa gcagcatatg cttttgaacg aaactatgaa
      361 cgtccggcag cgacacatcc tgaacgtcaa gattatgcag ttaactggta taacaaattt
      421 aaagatttaa aaccaggagg agctactgga gaagcaggac taaaacattt agagtctttg
      481 attggacaac gaattggcaa tggtcagtgt tatggcttgt ctgcagaata ttcaggatat
      541 ctgggtggtt gtggcatggg tgctggaaca aaatatggtt taactcatgt gataggaaat
      601 acttctgcag catctgatat tggtattgcc tatgattggt ctgctgttgg ttggaaagtg
      661 attcaaaacc ctagatatga tcaattagta gttggtgcaa ttattaattg ggcaagaggt
      721 ggacaagtgg gttcatggtt tgcagacgga acttatggac atactggcgt tatccgaggc
      781 ttagctaatg gtcgtatgca aacttatgaa caaaacacag agttaggtat gatttgtgga
      841 aagttagatc gccagtatta tagtgcaagt gcaatttctt ccattgtcat accaccaaaa
      901 tagggaggtg atttaatggc aaagtggaat gtcatattaa gtacaacaga accctataat
      961 tacgtgggga tgattcaagt tcgacaaggc aataaaaaca ctgaagttat ggaagctaca
     1021 attgtcgaaa atggtcttcc atatgattta tcagaatgta aggtatattt tgaatcagtt
     1081 gttggcggaa aatatccagt ccaattagaa acaagaattg tggatgctaa aaaagggaaa
     1141 atcaaatata tttttgataa atattccatg cagtgtttgc atcgacaaac agccaatttc
     1201 attatattta aaggagaaga cttgattgga acaactcaag acttctccta ttttgtcatc
     1261 aatgctgttt caaaaacaga aggagaaatg ggttcttatt ggcaatcaat tgaagattta
     1321 attgctgata tgacagattt tattaatgaa aataaaggcg attttacgga ctggatgaat
     1381 gaaaggaaag aagaatttga tcgctggaga gaagaacagg agaatagttt ccaagattgg
     1441 agagaaggac aagaatcaga ttatttgtca tggtttgaat caatcaagga tattttgaag
     1501 tctatcgatc caggtggagt aatgttagcc gaattaatgg atgcacgtgt agatcttcaa
     1561 ggtgtacgcc atgaatcgat ttctgaacgt ttcctatccg atctaaatta tttgtatcaa
     1621 aaaatgaaag cagcactttt cacgattgaa tacggtgaaa ttgaagtgac tgatattctt
     1681 caagacgatc tcttttca
//
LOCUS       DAFEOM010000164         1685 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.173, whole genome shotgun sequence.
ACCESSION   DAFEOM010000164
VERSION     DAFEOM010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.173"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(45..779)
                     /locus_tag="LP524_002868"
     CDS             complement(45..779)
                     /locus_tag="LP524_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBL2235849.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1106..1516
                     /locus_tag="LP524_002869"
     CDS             1106..1516
                     /locus_tag="LP524_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBL2235850.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1509..>1685
                     /locus_tag="LP524_002870"
     CDS             1509..>1685
                     /locus_tag="LP524_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBL2235851.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYN"
BASE COUNT          536 a          294 c          325 g          530 t
ORIGIN      
        1 ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc cataaataat
       61 aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac tggtacccat
      121 gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg tacaagacgt
      181 ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag tgaagcaact
      241 aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc ttggaagaat
      301 ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa gaattcattg
      361 acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc taacacatgg
      421 tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat caattgtcca
      481 atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca agggaataga
      541 ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct gattccacca
      601 aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag catccagtca
      661 ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt acctagaacg
      721 attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga gtaatccata
      781 aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg aaaagcatcg
      841 gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat aaaaaaatga
      901 attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt taaaagttct
      961 ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt tttctcaaaa
     1021 attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta gtaaaatgaa
     1081 agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca ttattatttt
     1141 cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc ctggaagtat
     1201 cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc ctgaaaaaat
     1261 cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc ctgctggtgt
     1321 aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac tgggagcagt
     1381 cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag gtgtagaagc
     1441 tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg aaatggagga
     1501 gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg actttaggag
     1561 tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg aatcctctcg
     1621 tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc tataaagact
     1681 ataat
//
LOCUS       DAFEOM010000165         1677 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.174, whole genome shotgun sequence.
ACCESSION   DAFEOM010000165
VERSION     DAFEOM010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.174"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(366..1658)
                     /locus_tag="LP524_002871"
     CDS             complement(366..1658)
                     /locus_tag="LP524_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBL2235852.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
BASE COUNT          516 a          342 c          285 g          534 t
ORIGIN      
        1 gtatgggcta ctctgtgata cttattagcc attaaactat tttacccaca tagcatatat
       61 tttgaaatcg ttcattccgt aaattcaact aaatatgcct aaaaaaataa aaaatctaag
      121 atagaataag atttattcag tcttagatgg atttatagca actttttcgt aacaacttta
      181 aaaaatacga aaagaaagag ctgttttact ctgactacgt tgctaaattg tcgtttaatc
      241 attcatttaa accaaatctt tttttataga attcgtttta atcctcctga atccaaaaac
      301 tatgaaaacc gtaattaaat agttaagagt ttttaaccac cataattctc tcggctattt
      361 tgaaatcaga taaattttga tcggacacat agttgaataa acctaccgtt gttacggaag
      421 gtaaatcgca tacttttcat tctagaggag aaggtctcgt tgatcattaa ctgattaagc
      481 accatagccc agttttgaat tcgaccacct gaccacttcg cgtgtaattc tttcgtgcgt
      541 aaataaagta gttttaggag tgcattctca ttagagaacg ctcctttttt tgtgactttt
      601 ctgaagctgg agtggacact ttcaaccgca ttggtagtgt acataatttt tcgaatggca
      661 ctaccataat caaatagttg ttcaacatgt gcaaagttcc gtttccagac atctacagca
      721 ccagaataat gagaccaccg attttgaaaa ctgtcaaaag cagcatgtgc agcgtttaga
      781 gaagaagcac cgtagaactt tttcatatct cggcagactt ccttatagtc cttacttgga
      841 atatagcgca atgcatttcg aacaagatga acaatacagc gctgaacaat taccgatgga
      901 aagatcgctt ttgcgccttc ttcgaggcca gaaacaccgt ccatcgaaat gaaaaagaca
      961 tcttcgacac cacgtgcttt cagttcatca aatacttgca tccagcgatt tttagattct
     1021 gtttgattta accataatcc taaaatctcc ttatttcctt tgagatcata gccaagaatg
     1081 gtgtatacag catattcttt ggcttcataa ttttctcgta aagtaacata catacaatca
     1141 acgaatagaa aggcataaca ctttgctagg gggcgggctt gccattcttc caattcagga
     1201 aggacagcgt cagtgatatc tgaaatcatt tcatgggaaa tatcaaagcc ataaatagct
     1261 tcgacggttg cggcaatatc tcgttgactc attcctcgtg catacatgga aagaaccttc
     1321 ccttcgatgt cggagacatc tcgttttctc ttaggaatta actctggttc aaaggaagct
     1381 tcccggtctc taggaacatc aatagctact tcaccaaaac tggttttaag cgttttagtt
     1441 ccatagccat ttcgacggtt atcgtgttcc ttaggctctt tagaatgggc atcataacct
     1501 aaatgattat tcaattctcc ttgaagcatt ttttcaaaaa ggggcccaaa cacatctttc
     1561 aaagcatctt gcatgtcatc gacagattca ggttgatagg cattcagaat ggattcagct
     1621 aacttttcgg catcaggatt tcttttcttt ctagccatcg tgtaatcacc tcttgat
//
LOCUS       DAFEOM010000166         1639 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.175, whole genome shotgun sequence.
ACCESSION   DAFEOM010000166
VERSION     DAFEOM010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.175"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(186..1505)
                     /locus_tag="LP524_002872"
     CDS             complement(186..1505)
                     /locus_tag="LP524_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HBL2235853.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          486 a          301 c          325 g          527 t
ORIGIN      
        1 ttaattcttt ggcacatttt attgaaatat gtatcactaa tatccctatc aactgatttt
       61 gactaaaaag ttacttcaaa cattcactgt tcaaaaatat tgaggaataa attttgggtg
      121 taaggcattt tgagcatact tctccctgga taacttccca gtaaaaaaat cgttagattg
      181 ttggattaga tccattgact ggctcgcctc aggcgagcaa agaccctgta gaattcattc
      241 tgatacacat gataactaga taatttgaga tagactcgtc tacccgtttg gactaatttc
      301 ccagcaactt tcagaaactt caatcgaata gaatgaatgg tcattccctt ttggacttct
      361 tcaaagccaa tcgttcttaa gaagttgact aagttgtaag ctatcaagct gagcatcatt
      421 cggacatgat tctccaaaaa gcgaggactg tctgttttgt caaagtaaaa gccagctttc
      481 gcttctttaa tgaagttctc cattgtgcca cgtttggcat agatagaaaa gatggtatca
      541 ggagaaacat tttctgatag attcgtcacg ataaattcat gtcgaaagag tagttcgccc
      601 gcttcacgtg ttgagcgtat acacacgcga cgactttgtg accaggattg tgcttgataa
      661 gtggtcgaga agtactgaac ttctcgttct tcccactttt gattatcgcc ataaagtact
      721 gatttctcag ctatttgacc tagtctacga ttattcttca gtcgaataac ataatgactc
      781 ttttttgatt cacacgaatc atacacacca ggtgtagcga acccgctgtc tccacgaacc
      841 aagatgtcag tgtttggtag agagtgatta tagtgctcta ataaaggtgt gagaaactcc
      901 tttacgcctt ttgatgtata ttgatttcct gaacgtagtt cagcttttaa gaagtcacca
      961 gtcaatccgt caaaagctac caatggatgg taaccatagg tttggtagtg tgtattataa
     1021 ttcgtttgtt cttggtgacc aaatgtatct gaatgggtcg aatctaaatc aatgattaat
     1081 tccgtatcat tacggatgag gcgtgcttta tcgataagtt cttggttcaa agcttgaagt
     1141 tcatggatat tctcctcaga tagtcgatct aaaaatcggg aaagtgaaga ttgagaagcg
     1201 agttctttcc tatctaaaac agctttaaac acaggatctt gtctcaggag attagctgca
     1261 gaatcagctg agtaaccagc aattaattgc ataatgaact gctcaagtat ggataagttg
     1321 tcatgagtaa aatacgctcg ttcgtcttca atgtgaatgt gttgcttagc cagttcagaa
     1381 aaaccaaaga tgttcatcag ctctttcact aggacgagac ccgaatcact cgataattga
     1441 ccacctgtat gagaaatagt gatatttgaa ttgaatttta cttggttttt gtgtaagcta
     1501 gtcatgaaaa gaactccttt cttttggttg gtttagtcac tttaaccata gcagaaaggg
     1561 gttctttttt catcacttaa cgggtgaaga tgaaaaagta attgtaagct gccgtgaggc
     1621 agtttcacat ggttttaat
//
LOCUS       DAFEOM010000167         1629 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.176, whole genome shotgun sequence.
ACCESSION   DAFEOM010000167
VERSION     DAFEOM010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.176"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            102..809
                     /locus_tag="LP524_002873"
     CDS             102..809
                     /locus_tag="LP524_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235854.1"
                     /translation="MNNVVYQLTMEDISRFLTNKEHLNEVIRRIQKDFSCMEEMKYFV
                     QNVEEQLYLESETSQTKVTVSNNRLDVYKRRPESLKKGAELLRLETEKYVMNVYLEKS
                     ELVENFISYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGIDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKQMPYQRFERKKGQ"
     gene            1190..1369
                     /locus_tag="LP524_002874"
     CDS             1190..1369
                     /locus_tag="LP524_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235855.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFNIPALKK"
BASE COUNT          633 a          240 c          319 g          437 t
ORIGIN      
        1 aacgaaaaaa gccaagactg tttcaagtga atggttagat tattttgaaa atatcaataa
       61 gtatggcgcc tattacacca tgaaaaagga ggacaacgaa aatgaataat gtagtttatc
      121 aactaacaat ggaagacatt tctcgttttt taacaaacaa agaacatttg aatgaagtaa
      181 tcagacggat tcaaaaagac tttagttgta tggaagaaat gaagtacttt gttcaaaacg
      241 ttgaagaaca gttatatttg gaaagcgaaa cgagtcaaac aaaggtcaca gtcagcaata
      301 atcgtttaga tgtgtataaa cgtcgtcctg aatcattaaa aaaaggagcg gagcttctac
      361 gactagaaac agaaaaatac gtcatgaatg tttacctaga aaaaagtgag ttggtcgaaa
      421 attttattag ctacacgatt tgcaggaaac actattactc agaatcagaa tattattcaa
      481 tttttgagag catggaatta atggaagaag aaatggaaga gttaaacagt aaagatgcga
      541 cggtagacga ttattacctt gtatttacta gatttttaac gtgtggaacg gaatctatcg
      601 tgctaatggg aatagattac gaactcgctg tacccgaagc gtttttgagg gaatatgtag
      661 aagaagtaga taacccaggg gatgattggt acacgacttt aactgatttt ttagacacct
      721 acacttacga cgatacagaa gaaatccaaa atgagcttga ccgtaaacaa atgccatatc
      781 aacgatttga aaggaaaaaa ggtcaataat catttatacc gaaaagtgag aatgtgtgaa
      841 ataagaacaa aaaaccttga aattaaggga atggttctta tttcgcacca atcgctccaa
      901 ccgcccccgc cgcttaggtg gtgcgaaata agcgagcaaa gcccgctaac caaagagaaa
      961 gaaggagatt tatatgaaaa agattattcc cttattatta gtactatttg ttggcgaaat
     1021 gcttatgcgt catgctgcaa ctgtgcattt aaacttcgta gtatttttgg taatactggt
     1081 gttttgtttg accttttggc ggtttgtatt agccctcgtg agtttactat tcaagatgat
     1141 tgcagccata tttttcatag gattatttcg atgagaggag agagtatcaa tgccaattgc
     1201 aataggaaat aaacggttac ccgttacgtt ggatgaaaag agacaaaaag aattacagca
     1261 acttaaacag aaatacggta aaagtgaatc caggattatg tgcattgcgt tagatttatt
     1321 gattgcgcag gaaaaagcag gattcaacat accagcgctc aaaaagtgac gtcacttttt
     1381 gacttaaaaa ataaaagtga tagcactttt atatagtaaa aagaagaata aacgttaagg
     1441 gatagaaaaa gagaaaagaa caaaaaaaga gctaacagta ccattgttag ctcttatgca
     1501 gtaatgaaaa ccaagccttt aaaaaaggag atgtttcatc acaaacacaa acagaaaaat
     1561 aaaaaaatca aaaatctaat aatcataaat tgctaccgaa gcacctttta ttataattaa
     1621 tcaaatata
//
LOCUS       DAFEOM010000168         1502 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.177, whole genome shotgun sequence.
ACCESSION   DAFEOM010000168
VERSION     DAFEOM010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.177"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(312..611)
                     /locus_tag="LP524_002875"
     CDS             complement(312..611)
                     /locus_tag="LP524_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235856.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(617..847)
                     /locus_tag="LP524_002876"
     CDS             complement(617..847)
                     /locus_tag="LP524_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235857.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(1097..1297)
                     /locus_tag="LP524_002877"
     CDS             complement(1097..1297)
                     /locus_tag="LP524_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL2235858.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          533 a          243 c          219 g          507 t
ORIGIN      
        1 attgtgagat aataataaag aagagtttaa agcgttcccc aaaaaccact gccccataag
       61 tgtgttacgc tttaaactct tttatatttg aagccattaa aaagcatacc atatttttga
      121 aaaaaagtga gaaaaaaggc ttataattgg agtggtagtt aattagtgac ttatttttga
      181 ttttatagca ctgatactat aaaatataga tatcatcata ttacacaatc ttaatactaa
      241 cttaaaaaat atctcctttc ataagtatgg tgataaaatc cgttccgggc taccttttta
      301 ggtagcctac tttaatcttt gtatctttct ggatcaacga aagtatactt tatatagtca
      361 taacgccgat gatcgcttcg agcgtctagc acatcagtca cgatatcaaa caaaaaatat
      421 acgtctttct tcattctagt tttcgcagca gggattttaa agtagttctt attagaatag
      481 tagagattaa ttaataagct atcttcgatt gctaaaaaga aaacttctga atcccacacc
      541 ttataaaaat ctttgacaaa tctattcgaa ggatcaaatt taaaccataa ttgcgtctca
      601 ttaaaaagca taaccattac tccgatctat ttttaaactt agtctctacc tctaatatat
      661 atcgagtttt tattttgcct tcagagaata ctgtttcttt ttttgcagtt acaggttgtt
      721 tattttcgga aaaagctaat atagctaaaa ttgaaacatc catctggaat ttgtcttttt
      781 cgttgctttg ctcataaaaa tctgcatatt catcactaat attttttcta ataaattctt
      841 ccatcataaa gatcacctcg aaaagagtat acgaacaaac gttctttttg taaaggtgga
      901 atttattata ctaaataaat aggtgaacaa gtacttgtgc caaattatgt gccaaaaaaa
      961 atcgaattta ataaaacaca aacaaaaaga aatcctatta tgataacatt tcttataata
     1021 acaaacacaa cagaagacgt gtaatagtta gtcaggaacg tacaaataac ccctgtatcc
     1081 tttgcggtac agggggttat tttgtataag tagacattgt agtttctaaa ctgctatgtc
     1141 ctaaacgttt cgatacgcta agtatattta ctccttgata aagtaaaata gatgcatgcg
     1201 tatgtcttag tccatcaata aacagtcgaa gagaaaatag ggaaaaattg attactaaag
     1261 aaaaacttac taggtcgacg gtgtagttga ttttcatact tcaatagatt cgaattaaac
     1321 gatttcgatt ggcttatacc agtcctatcc aacgaaagat atatcgttct taactctagc
     1381 ggtataactt gaagctttac gtaaagccaa tgggaatgaa ctggagatga ctttttatta
     1441 tttttagtaa tagctgatgg gttaatactt acaactttcc ttttcaaagt accatataaa
     1501 aa
//
LOCUS       DAFEOM010000169         1456 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.178, whole genome shotgun sequence.
ACCESSION   DAFEOM010000169
VERSION     DAFEOM010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.178"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            108..1040
                     /locus_tag="LP524_002878"
     CDS             108..1040
                     /locus_tag="LP524_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HBL2235859.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1112..>1456
                     /locus_tag="LP524_002879"
     CDS             1112..>1456
                     /locus_tag="LP524_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235860.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          640 a          169 c          227 g          420 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      901 atcaaagaaa agaatttttc aaagtttcac tagacgaaat agagaaagtt gttttagaaa
      961 attataatgg aacagtaaca tttacaaaat tagctaaagc tgaacaatat agacgatctt
     1021 tagaattgtc aaaagattaa atatataaaa agccttcggg ctttttcttt taacaattaa
     1081 taggaacgta tattcgaaag gaatagaaat aatgtggtta gaaaaaataa atgacaaaaa
     1141 atttaagtac accgaacgtt atacggatcc cttaactgaa aaaaagagaa aagtgtccgt
     1201 tactttatct agcaattcac gccaagcttg gaatcaagct aacttacttt tgaatgaaaa
     1261 aatcgcagaa aaaataaaac gaaatgaaga attaccacta acttttggag aattaaaaac
     1321 taaatgggat gaaaaataca agccaactgt aaaagaaagt tcctatcgaa caactcaggt
     1381 ttatctatca ttaatttcaa aatatataaa agatgatgta cttgtaaaaa acgttaatag
     1441 taatcttata caagat
//
LOCUS       DAFEOM010000170         1445 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.179, whole genome shotgun sequence.
ACCESSION   DAFEOM010000170
VERSION     DAFEOM010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.179"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(107..970)
                     /locus_tag="LP524_002880"
     CDS             complement(107..970)
                     /locus_tag="LP524_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBL2235861.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
BASE COUNT          405 a          315 c          256 g          469 t
ORIGIN      
        1 aaaaaaacag cgacctctaa gagataggat gctcatctat cttttaagag tcgctgtttt
       61 tgttacaatc aatttctggg cttttttaca gaatgagtta ctgcttttat cgactgattc
      121 ccatcgaaaa aatcgcgata cagccatagc ctgtgtgact ggcgatcgtt ggtcctaagg
      181 gataaagctt cacatctttc atctttatct gctcttccaa aagccgtttt gccttctccg
      241 cgctctctag atccccagcg taagccacat aaacgatttg atcaagtggt gcaacgattg
      301 tttttaccgt ctcattgacg attgtctgga tcgacttgtt tcttcctcga actttgtcaa
      361 cattcgtcag ccttccttct ttatccaccg taataatagg ctttacactc aatagactgc
      421 caagagtagc acttgctttg gaaatcctac cgcctcgttc tagatatttc agatcgtcta
      481 ctgttaccca tgaatggact ttcaaataat tactttccag ccactctagg gtttcttcga
      541 tgcttttacc ttcttttttc aatcttacag cttccaatac taaaagccct tccccaatac
      601 ttgcagcttt tgtatcaaaa acatagatct cagggtcttc atattcttcc ttcagcaatt
      661 caaccgcttg aagcgcacta ctgtaagagc cgcttaatcc actggaaaaa gccacatata
      721 aaacaggaat ctgtttttcc gcataagaac ggaaaaattc taaatatcgg ccaacattga
      781 tttgtgatgt cgtaggcatc gcaccatttt tcagctgatt catgtagcgg tcataattga
      841 atgtctttcc gaggtcatct actaactctt gatcattcaa ttggagattc attgataaaa
      901 aatcaacttt ttcttttgct aatacatggt atggcagatc acaacaagaa tctgttacaa
      961 tttgatacac atttttcaac cccttctcgt tacattttac ccttatcgtc tattatagca
     1021 aatctcttgc gctagacaca ctttaaccgg tttattttcg caagttctaa tcaaaataag
     1081 aggttgtgac agaagtggtc agcttcaaga aataagaagg aatttacgaa aattgcttct
     1141 caaatttttg tgaaatttcg gcttatttcc gtagaagcta cttctggaac accgtgaatc
     1201 agaggttgtg aaatcagcgt tttgacctga gtattagaga aaaaaacgga aaaaatagtt
     1261 cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa caccgtttac
     1321 caagaggttg tgagcctgcc gtttagctcc gagtcacaag gaacaatttc aaaaaacagc
     1381 ttctcatgtt tgttgaatat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt
     1441 agtgg
//
LOCUS       DAFEOM010000171         1421 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.180, whole genome shotgun sequence.
ACCESSION   DAFEOM010000171
VERSION     DAFEOM010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.180"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            96..1388
                     /locus_tag="LP524_002881"
     CDS             96..1388
                     /locus_tag="LP524_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBL2235862.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT          462 a          291 c          257 g          411 t
ORIGIN      
        1 accagcatct tttttatttt ccaaaacata tagaaaagac accttgaaat cctttagaat
       61 taatttggcg aaccaaccca aaaggaggaa tcaagatgtc ttacactcac cttatcaaag
      121 aaacattaga tattctagac ttatcagtta cttttaacga aaattgtctc acaaaagaaa
      181 aatacaaagg gcaaatctgt catatttatc gtggaaactt gatttataca gcccaagaat
      241 gcatccactg taaacaccag attgcttcag atattgtgcg ttggggtaca acaaccgtcc
      301 ggttgttaat gaacgatgtt tctgaatacc gtacctacct cgaattgaaa aagcaacgct
      361 tcaaatgcaa agcttgtcag cggacctttg tcgcagatac ttctgttgcg aaaaaacatt
      421 gtttcatcag tgagaaagtc cgctggtctg tcgtgactcg tctgaaaaaa aatacctcaa
      481 tgacagagat cgccgctcag aaaaatctat cggtctcttc tgtttaccgt attatgaagc
      541 gattttatcg tccgttgaat ccttttcgga caccgttacc taaggtactt tgttttgatg
      601 aattcaaatc agttcggggt gtttcaggtg ctatgagttt catcatgatg gatggacaaa
      661 cacagcgctt attggacatt gtcgagaacc gacaactgcc ttttttaaaa cgttatttta
      721 gtcatttttc acgagaaatt cgtgaagcgg tcgaatggat cgtgattgat atgtacgctc
      781 cttatgtatc gctcgttaaa aagctgtttc caaaagcaca gctgattatt gaccggtttc
      841 acatcgtcca acatattggt cgtacctttc gcaatcatcg aatcaaagaa acgaaccaac
      901 tattaaaaag caaagaacaa aaacactatc aattaggtaa gcaattaaaa agatactgga
      961 aacttcttca aaaagatgaa cgcaagcttg attatactcg acgcctttgg cgacctggct
     1021 tcaaagctca tttaacagag accgatattg tggatcgctt actgaaaggg agccctgctt
     1081 tgcgtgtagg ctatcagtta tatcaagact ttctttacgc tgtaaaggaa cgagactacg
     1141 tatcgtttga agaactactt accaataata tcatgcttcc cgaaggctat cagacgacct
     1201 tgagaacgtt ccagaaattt ctgccacaaa tcaaaaatgc tttacaacaa agttattcaa
     1261 atggcccact tgaatgtctg aacaaccata tcaaagtgtt aaaacgaaat gcatatggct
     1321 ttcgtagctt ttataatttc aaattgcgca tcatgattcg tcatggcaac gcattgattt
     1381 tcaactaaaa aagatcaaga ggaactgatc cacttgatct c
//
LOCUS       DAFEOM010000172         1392 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.181, whole genome shotgun sequence.
ACCESSION   DAFEOM010000172
VERSION     DAFEOM010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.181"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            57..1358
                     /locus_tag="LP524_002882"
     CDS             57..1358
                     /locus_tag="LP524_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBL2235863.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          454 a          279 c          257 g          402 t
ORIGIN      
        1 atacagaagc tccgttagaa ttgaatcacc acaacacaat caaaggagcg ttctgtatgc
       61 ctgatcttca tcatatcaaa ctactacttg gaatcaaaga taaaaacatt ttcatcacaa
      121 atgtggaatc gaagagcatt aagaatatca agagtcttgt tgtttctgcg acgttaagta
      181 aagaaattcg tcgctgtcct ttgtgtaaac agatgaatca tgaaggcatg atcgttaaga
      241 acggaaagaa aaaatcgctc attcaactta ataaatgtgc gaaccaactg acttatcttg
      301 ctttagcgaa acaacgctac cactgtcgtg gctgccacac ctattttacg gcgaatacct
      361 acattgtcga tagaaactgc tttatcgcta aacaagtgcg ctataaaatt ttagaagagc
      421 taactgaaaa acaagcaatg acgacaattg ctaagcattg cggcgtttct tggtcgaccg
      481 tttctcggac actcgcttct ctgatcccca tgacaaaagt aaaaagaaat tggttaccgc
      541 gttgtttatt aatggatgaa tttcgttcac tgaaaaatca agtcggtccc tactcctttt
      601 cttgtatgga tggcgatact ggaaaattac tggatattct tccttcacgg aagaaaaagg
      661 ctctcgtttc ttattttatg caatttgaac gacgcgcacg attgaaggta aaggtcatcg
      721 tgacggacat gaatgtttct tatgcctctc tgataaagga atgttttcca aaggcaaaac
      781 tcgttgtgga tcgttttcac attgtcaaac atctcattcg taaatttgag gatattcgcg
      841 tacgaatcat gaaaagcttt gatcgaaacg atccaacaca agccaaatat tatcgacaat
      901 taaaggcgtt gtctcggctt ctgatcaaac gacaggacaa gcttgtttat gacaaatgga
      961 ccaaatggcg caattttggt tgggcgtatc tcaccgaaag tgaagtggtg gatcgtctgc
     1021 tttcgatctc tgacgaatta cgaatcgcct atttttacta tcaagaaatt cttcaagcgt
     1081 ttcacgacaa agaggcggac actttcttta aacttgtaag aacgatgccc aattccgttc
     1141 caaaagaact gcattctata aaaaaagctt ttataaacta tgaatccggc attcgtttag
     1201 ccctcgaatt gccttattca aatgccaaaa tagaaaatct gcatacccac atcaaagcat
     1261 tgaaacgagt cgcttatggc tttcgaagct ttcggaaaat gaagaccaga attttcttac
     1321 taaataatct gattacctat gaatcaaaaa acatctaaag tagaaaagga attctacctt
     1381 agatgttctt at
//
LOCUS       DAFEOM010000173         1374 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.182, whole genome shotgun sequence.
ACCESSION   DAFEOM010000173
VERSION     DAFEOM010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.182"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            33..1372
                     /locus_tag="LP524_002883"
     CDS             join(33..474,474..1372)
                     /locus_tag="LP524_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HBL2235864.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          576 a          177 c          239 g          382 t
ORIGIN      
        1 gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga
       61 aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat
      121 atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg
      181 aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat
      241 atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa
      301 atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga
      361 gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta
      421 aagatgagtc tcttgaagaa gaagttattc gtttaagagc agagaacgca tatttaaaaa
      481 agttagaaac cttgattcaa gaacaagatt tgagcaagaa gaaatcaagg ttaaaacaat
      541 ccttcagctc aaaaaagaat ttaaattgaa tctattactt tcaattgcac aattagcgaa
      601 gtcaacttat tattactggg taaaaaaatt agataagcca gataaatata gcaagataaa
      661 gcaagaaatt acagcgattg taaaagaatc aagaaactct tatggttatc gtagagtcac
      721 tttagcgtta aagatgaaag gatatacaat caatcataaa acagttagaa aattaatgtc
      781 ccaaatggga cttacctgtc aaatccgaat aaagcgatat aaatcttata aaggaacagt
      841 aggaaaaatt gccaagaatg tgttaaaacg aaatttttca gtagatacac ccaataaaaa
      901 atgggtgaca gacgtcactg aattcaagat aaaaggaaga aaaatctatc tatctcctat
      961 acttgattta tttaacggag aaataattag ttatagtata tcaactagtc caacatataa
     1021 actgattgaa gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg gttcactgat
     1081 tctacattca gatcaaggat ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga
     1141 gaataacatt attcaaagca tgtctagaaa aggaaattgc ttagataatt ctgtaataga
     1201 aaattttttt ggtgtgctta aatcagagtt tttttatcga gaaaaattcc gatcaattga
     1261 gatatttcaa agtaaattaa atgagtatat taggtggtat aataacaaaa ggataaaact
     1321 aaagttaaat ggactatctc cagtagaata tcggaaacag tccattaaat agtt
//
LOCUS       DAFEOM010000174         1360 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.183, whole genome shotgun sequence.
ACCESSION   DAFEOM010000174
VERSION     DAFEOM010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.183"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            82..>1360
                     /locus_tag="LP524_002884"
     CDS             82..>1360
                     /locus_tag="LP524_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HBL2235865.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQ"
BASE COUNT          463 a          262 c          257 g          378 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat
//
LOCUS       DAFEOM010000175         1348 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.184, whole genome shotgun sequence.
ACCESSION   DAFEOM010000175
VERSION     DAFEOM010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.184"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            15..1310
                     /locus_tag="LP524_002885"
     CDS             15..1310
                     /locus_tag="LP524_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBL2235866.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          418 a          268 c          260 g          402 t
ORIGIN      
        1 taggaggatt caagatgtct catactcagc ttatcaaaga taccctaaat attctagacc
       61 taaatatcta tttcgaggaa aattgtttaa ctaaagaaaa atacaaaggg caaatttgct
      121 tgatttacaa aggaactctt ctgtacaagc cagaagagtg ttcccattgt ctttgtgtcg
      181 tcccctctag gatcattcgt tgggggacga caaccgttcg gttgttactg aatgatgtgt
      241 ctgagtatcg cacttatctg gagctgaaga aacaacgatt taaatgtaag tcttgccaaa
      301 gaacattcgt agcggatacc tctgttgctg aaaaacactg tttcattagt caaaaagttc
      361 gttggtctgt cattgctcgc ttaaaagaga atacttccat gacggaaatc gctcgtcaga
      421 aaaatatttc cacttcttcg gtctatcgtg tcatgaagcg attctaccgc ccattgaatc
      481 ctttcaaaca gactttgcca aaggtcttat gctttgatga attcaaatcg gttcgaagtg
      541 ttgccagtgc aatgagtttt atcatgatgg acggacaaag ccatgcatta ttggatattg
      601 tcgaaaatcg acgcttgcct tatttagaac gctatttttc tcggttttca ttagcgactc
      661 gtgaagccgt tcaattgatt gttattgata tgtatgcgcc ttatgtttcg ttggtgaaaa
      721 agcttttccc gaatgctcag ttgatcatcg atcgtttcca catcgtccaa catattggca
      781 gaactttctt gaatcatcga gtgaaagaaa ccacggtacg acttaaagga aattcccact
      841 ctcaacgagg gttagggaaa aaattgaagc gctattggaa actcctacaa aaagaagaag
      901 caaagttaaa ctatgaaaaa cgggtttggc gtgccagttt taaagattat ttgactgaaa
      961 gtgagattgc cgatcgactg ttagctgctt gtcccaactt acgccaaggc tatcagttat
     1021 accaagacat tctttatgcg gtaaagaaaa gagatcagtc actatttgag gattgtttaa
     1081 ctagagacat tagcggtctg ccagaaacct atacaaccac tttacgaacg tttaaaaagt
     1141 ttctgccaca gatccaaaat gcgcttcact acagctattc aaacggacca ttggaatgct
     1201 taaacaacca catcaaagta ttaaaacgca atgcgtacgg ctttcgcaac ttttacaatt
     1261 tcaaactacg aattatgatt cgtcatggca aaacattttt aaccaaataa acagaacaga
     1321 aagaggtttt cttctttctg ttctgtcc
//
LOCUS       DAFEOM010000176         1342 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.185, whole genome shotgun sequence.
ACCESSION   DAFEOM010000176
VERSION     DAFEOM010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.185"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            211..1083
                     /locus_tag="LP524_002886"
     CDS             211..1083
                     /locus_tag="LP524_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL2235867.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          418 a          234 c          294 g          396 t
ORIGIN      
        1 attaatcaaa tatttgagaa taacatttta tttaatcttt tcatatatca attgtatgaa
       61 aaattattag aattttaaaa aatgataaaa attcaaaaag aaaaaatagt agttatttac
      121 ttgtcatgtt actaaataaa aaaataattt aaatatgata tatagtcatt tatttagtga
      181 tttcattcta aaataaaggg agaaatgtct atgctatacg gtgcaattga agcaggcggc
      241 acaaaatttg tgtgtgccat tggcgatgaa gaaatgacga tcaaggaaag agtgagcttt
      301 ccgacaacga cacctgagga aacaatgcct ttagtcattg acttcttcaa acaataccaa
      361 gcagatttag cagggattgg gattggctca tttgggccta tcgatatcca tcgcgattcc
      421 gccacttatg ggtacattac ctcgaccccg aaattggcat ggcaaaattt tgatttcatc
      481 ggtaccatga aaaaagaatt tccgatcccg atttcttgga caacagatgt caatgcggct
      541 gcctatggtg agtatgtctt cggcagtggc aaaggattga gcagtgtcgt ttactatacg
      601 atcggtacgg gtgttggcgg cggtgcctta caagaagggc gctttatcga agggtttagt
      661 catccagaaa tggggcatat gctcgtcgtc cctcatccaa aagatgactt tgctggcagc
      721 tgtccatttc atggcaactg tttagaaggg atggccgctg gacccgccat cgaaaaccgc
      781 ttagggaaaa aaggacaaga ggttgcagaa gatgatcctt attgggaaat cgaagcctcc
      841 tatattgctc aatgtgccta caatacaacc ttgatgtttt ctccagatgt gatcattttt
      901 ggcggtggtg tgatgaaaca acgccatatg ctgcaaaacg tccacgacgc atttgcaaaa
      961 ttggtcaatg ggtatgtcga aacacctgag ttaagcaaat atatcgtgac cccagcatta
     1021 gaagacaacg ctggaacgct cggttgtttg gcattggctc gagaagcggt cttacattca
     1081 taaacgaaaa aaggctcttc gacatttagt gttggtagag aaagatacaa ctgaaaattt
     1141 agtcataacc agttaggcag agtgcttaac tggtttttgt tttacttgga agatcaagcc
     1201 ttgaatgaga taaattcttg ctcgaaagtt atggaaattt cgatagccgt aggccacttg
     1261 tattgtgcaa tagtttttta caaaaaaaag ggggtgcgga tatttttttg gaccttttta
     1321 acaaatatat ctttgcttct tc
//
LOCUS       DAFEOM010000177         1333 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.186, whole genome shotgun sequence.
ACCESSION   DAFEOM010000177
VERSION     DAFEOM010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.186"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            51..>1333
                     /locus_tag="LP524_002887"
     CDS             51..>1333
                     /locus_tag="LP524_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBL2235868.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTK"
BASE COUNT          420 a          289 c          265 g          359 t
ORIGIN      
        1 aaatccttta gaataaagtc gacgaaaacc attcaaagga gtgtttcaag atgtcctatt
       61 cagagtttac caaagagtta ctagatattc tagacctaaa tcttaccttt catgaagatg
      121 cctttcgcaa agagcgcatt aatgacgaaa catgttttgt ttttgatggc acattgacct
      181 atcaaccaga ggaatgcttc cattgccact atcaaaataa gcaaacgatc attaaatggg
      241 gctggaaaaa agtatccatc ttattgaatg atgtcagtaa ctacaagacc attgtgcgga
      301 ttaacaaaca gcgcttcaaa tgtaaacact gtggaaagac ttttttagca gaagattcag
      361 ttagcgatcg tcgctgctcc attgcccgca gagtcaaaca agccattctt gagctattaa
      421 gtgagcctat ttctatgtct ttgattgcac gtatgaagca catttctccg accactgtca
      481 ttcgaatact tcgcagcctt cggcctaaaa ccgtttcttt gaaccagccg ttaccagaag
      541 tggtgtgttt cgatgaattc aaatcggtga aaaacgtctc gggagccatg agctttgtca
      601 tgatggacgg caagacccat caactgattg atattgtgga aaatcggcaa ctcaatcctt
      661 tacgcgacta tttcttgcgc taccctagaa aagtccgtga acaagtacgt ctagtggttt
      721 cagatttcta tacaccctat cgcacattgg tgaaagagtg ttttccgaat gcccgaatcg
      781 tcgccgatcg ctttcatatt agccaacaca tcggtcgggc ctttaccaac caccgaattc
      841 aagtaatgaa gacctttaaa aaaggggatc gtcggtcgaa gcacttaaag aaatactgga
      901 gactacttca aaagaatgcg tgggaattga atggtcagca tcgctactgg cgtccgtcat
      961 ttcgagatca tctgacagaa gcggagatag tcgatcggct attatattat gatgactcct
     1021 taaagcgagg ctacgaagtc taccaggttt ttctttccgc tatcagaaga caagatgttc
     1081 ctgaattcgt cgcgctccta aaagaagact acaaggagtt gccagagcac tatcaaccag
     1141 tgttcacgac cttcaagaaa tatcgaacag aaatcaaacg agcgttgcga gttccctatt
     1201 ccaacggccc tatagaatgt ttgaacaacc atatcaaagt actaaaacga attgcctatg
     1261 gctttagaaa ctttcaaaac taccgagaac gaatcttttt atatcgagga aaatatttca
     1321 agaaaactaa gaa
//
LOCUS       DAFEOM010000178         1331 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.187, whole genome shotgun sequence.
ACCESSION   DAFEOM010000178
VERSION     DAFEOM010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.187"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            55..1217
                     /locus_tag="LP524_002888"
     CDS             join(55..331,331..1217)
                     /locus_tag="LP524_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL2235869.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTSGSFKEKDNLTPEQKELLELRKRNQQLEMENDILKQAALIFGPKRQVIDANK
                     HLYPISAMCRILGLSRQSYYYQSKPKKDESELEEVVAEEFIRSRKAYGSRKIKKALSK
                     RGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIV
                     TDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFH
                     TDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQ
                     QLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELGCANASEAV"
BASE COUNT          485 a          234 c          265 g          347 t
ORIGIN      
        1 gacagaaaaa ttaaggtgtg tagactagaa gaaaacatac caggaggaat ttttatgtct
       61 aagagaacac gaagaacttt ttcacaagaa ttcaagcaac aaatcgtcaa tctttactta
      121 gctggaaagc cacgtgtaga aatcattcga gaatatgaac taacggcttc agcatttgac
      181 aaatgggtaa agcaatctaa aacgagtggt tcattcaaag aaaaagataa tcttacgcct
      241 gaacaaaaag aattgttaga actacgtaaa agaaaccagc aattagaaat ggaaaatgat
      301 attttaaagc aagcagcgct gatattcgga cgaagagaca agtaatcgat gcgaataagc
      361 atctttaccc tatatcagcg atgtgcagaa tattaggtct atcacgtcag tcctattatt
      421 atcaatcaaa accaaagaaa gacgaatcag aacttgaaga agtagtcgct gaagaattta
      481 tccgcagccg aaaggcctac ggctcaagaa aaataaaaaa agccttatca aaacgaggca
      541 ttcagatcag ccgacgaaaa attagtagaa tcatgaaaaa tagaggatta aaatcgagct
      601 atactgttgc ttattttaaa gtacatcatt ctacttgcaa tgaagccaaa acgacaaacg
      661 tattgaatcg taaattctta agagacaacc cattagaagc gatcgtaaca gacttgactt
      721 atgtacgagt cgggaaaaaa tggaattatg tctgtttcat tttggatctg ttcaatcgag
      781 aaattctcgg ctattcttgt ggagaacata aagatgccgt tctagtaaaa aaagcattta
      841 gccgtatcaa acaacctctg acagaggttg agatttttca tactgatcgt ggaaaagagt
      901 ttgataacca agctattgat gaattattaa caacttttga catcaatcga tcattgagtc
      961 ataaaggctg tccttttgat aatgccgtag ctgaatcaac ttataagtcg ttgaaagtag
     1021 aatttgtcta tcaatacaca tttgaaacct tacaacaatt ggatttggag ttatttgact
     1081 atgtcaattg gtggaaccac cttcggttgc acggtacact tggctacgag acaccggttg
     1141 gttaccgtaa ccagagattg gcgcagcgaa tccttgataa tgagctcgga tgtgctaacg
     1201 ctagcgaggc agtctaactt taactgttag ctcctgccga agatcgtcac atccgaggag
     1261 gctcattgtc aaggacaatc ggaatagcat acggaaagaa gttctgcacc ttataaaatt
     1321 tgtcaaaaaa a
//
LOCUS       DAFEOM010000179         1300 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.188, whole genome shotgun sequence.
ACCESSION   DAFEOM010000179
VERSION     DAFEOM010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.188"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(<1..1095)
                     /locus_tag="LP524_002889"
     rRNA            complement(<1..1095)
                     /locus_tag="LP524_002889"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          289 a          363 c          248 g          400 t
ORIGIN      
        1 cctagttgtc tgtgcaaccc cacatccttt tccacttaac atatattttg ggaccttagc
       61 tggtggtctg ggctgtttcc ctttcgacta tggatcttat cactcacagt ctgactcccg
      121 gatatgaatg aatggcattc ggagtttatc tgaattcggt aacccgagat gggcccctag
      181 tccaaacagt gctctacctc catcattctc aattccgagg ctagccctaa agctatttcg
      241 gagagaacca gctatctcca agttcgtttg gaatttctcc gctacccaca cctcatcccc
      301 gcacttttca acgtacgtgg gttcggtcct ccagtgcgtt ttaccgcacc ttcaacctgg
      361 acatgggtag atcacatggt ttcgggtcta cgactacata ctcaaacgcc ctattcagac
      421 tcgctttcgc tgcggctccg tctcttcaac ttaacctcgc atgcaatcgt aactcgccgg
      481 ttcattctac aaaaggcacg ccatcaccca ttaacgggct ttgacttgtt gtaggcacac
      541 ggtttcaggt tctatttcac tccccttccg gggtgctttt cacctttccc tcacggtact
      601 ggttcactat cggtcactag ggagtattta gccttgggag atggtcctcc cggattccga
      661 cggaatttct cgtgttccgc cgtactcagg atcctcctag gtgtcttcca catttcgtct
      721 acggggtttt taccctcttt gactgacttt tccaagtcat tcgactatct gaaagaacta
      781 ccatattgga gtcctacaac cccaacaagc aagcttgctg gtttgggctt ttcccgtttc
      841 gctcgccgct actcagggaa tcgaattttc tttctcttcc tgcaggtact tagatgtttc
      901 agttctctgc gtctacctcg aatgtgctat gtattcacac aatcgtaaca tcctattaaa
      961 gatgttgggt tcccccattc ggaaatctct ggatcatagc ttacgtacag ctccccaaag
     1021 catatcggtg ttagtcccgt ccttcatcgg ctcctagtgc caaggcatcc accgtgcgcc
     1081 cttattcact taaccttatc aaccttgcgg ttgggttttg atccttcttc tagcgataga
     1141 agttagatca agaaaataag caattgaact tattaaaaaa ctctatccag ctcacaaatc
     1201 agcctgtgcg gcaaaaagat cgatcgtctc ataaaatcaa agattttata gccgtccgcc
     1261 tattttgctt taggctaaac aatttgttca gctttcaaat
//
LOCUS       DAFEOM010000180         1297 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.189, whole genome shotgun sequence.
ACCESSION   DAFEOM010000180
VERSION     DAFEOM010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.189"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            141..398
                     /locus_tag="LP524_002890"
     CDS             141..398
                     /locus_tag="LP524_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235870.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(630..1061)
                     /locus_tag="LP524_002891"
     CDS             complement(630..1061)
                     /locus_tag="LP524_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HBL2235871.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          490 a          165 c          162 g          480 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaatagat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tctcttttct taattgtaca attagtatta
      601 tctgcactac tttctaatta aataaatttt tagttattaa cttgattaaa atcagagata
      661 atatcattta attcagtttg aagggttgta acgattaaat ggaaattttc ttcatctgtt
      721 ttattatatg gggatgtttt cgttatatat aacaactttt ctttttcaat tttcattaat
      781 aactctgtcg aagtctgtac tttatcttga taattaaatg cagacatacc actttgtata
      841 attgtaatta ttgctgaagc aattgaaaca atcaatagta aagacctgtg ttcggaagcc
      901 tgatcaatta atattgggat actagctgat aagactattt ttataatatt tccgatacca
      961 attactctat tataaaatcg aatttctttt tttaatctat cgattgtaga atcaatagat
     1021 tcgataaacg ctttttgatc catcattttc tctccaatca ttttattaaa atcattaaca
     1081 aaaatattat aataggattt aagtaagttc taactaatca catataaaaa taaaaaaaca
     1141 tttgtatttt atcaaagttt tattatactt gtcaatttgt gtacatccta aaatataagc
     1201 tatttgttta cttctgtaga atatatactt tgttgttaga taagttgcac atgtttatag
     1261 aatctaaata ataaattttt atgtaccgcc cctcaaa
//
LOCUS       DAFEOM010000181         1295 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.190, whole genome shotgun sequence.
ACCESSION   DAFEOM010000181
VERSION     DAFEOM010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.190"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(238..387)
                     /locus_tag="LP524_002892"
     CDS             complement(238..387)
                     /locus_tag="LP524_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235872.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            849..1061
                     /locus_tag="LP524_002893"
     CDS             849..1061
                     /locus_tag="LP524_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235873.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT          480 a          195 c          218 g          402 t
ORIGIN      
        1 taaatattat acagataata acggaatatt atcatcgaaa cagctaacaa aattgtcata
       61 aaaaacgaga tggttgatat tagaacttgt gtttttgttt aggtctagag ataaagagag
      121 acaatttcca aatatatgta atgaatacaa aaagacgttt gtcgcggatt accgttacaa
      181 acgtctttta ttgattttcc gagatctcat cagtcgccat ttggaatggt tcactcctta
      241 tctagattcg tttaatgctc ggactgcttg ttcgattgta ataccaaacg caacgttatt
      301 ttcgttttgt gcatcaatcg ccataaaacg gttagaagtt gtatcaaaag ctacgcggta
      361 ttgcatattt gtttttgtaa acgtcataaa attcatctcc tttgtaatgt aatttagttc
      421 ttgaaagttt cctttaaact ttcgaactta taccaagttt agcatcgtac gttttttgat
      481 gcaattaaaa agcatttatc attttcctta tttatttttt cttaaatggt ttagctattt
      541 tcttttaatc cgataagtag cccaatcatc aaggttacaa atactattgc ttgaatataa
      601 atcatatgct cacctcttta taaataactt agcaatataa agaaaaaaac gcacgaacaa
      661 cataggaata taaacaaacg tttttgtcac aacgtctaag atcgtgttaa aaaatagtta
      721 tctaaaaagc agaaagatac tcaaaataaa aaataaaaaa atggataaaa acgttttcaa
      781 atacaaggta atttgttatt ataaaataaa gaaaaaacaa aataaaaata aagaaaagag
      841 ggaatgaaat ggtaaaagca gaagtaggaa gatcttatat atgcaaaccg atcggagtat
      901 ctcatgaact aatcggctgt attgaacgta catatatcaa cacggctgtc gtgtatgttg
      961 aaagttatga ccaacgagac tatcaactta tcaatgaaag caagtatcgc atgctcgtaa
     1021 aattctgtga tattagcgca cctgctgaat tggtttctta aacagtattt ttgagaattc
     1081 gcaagaactc ccttataata taaataaaga cagaagggag cgagaagata tgccaaaaag
     1141 aaaaagttca gggaaaatgg gaattattct tgcagtgatc tctgcaattc ctgctatttt
     1201 agctattgtt cgtgcgatga aggaatcaag agaaagttaa gcacacatta tgagcgtcaa
     1261 acaaaaaacg catatacctg cggtggcagg ttatg
//
LOCUS       DAFEOM010000182         1294 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.191, whole genome shotgun sequence.
ACCESSION   DAFEOM010000182
VERSION     DAFEOM010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.191"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            <1..1244
                     /locus_tag="LP524_002894"
     CDS             <1..1244
                     /locus_tag="LP524_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBL2235874.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DAFEOM010000183         1290 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.192, whole genome shotgun sequence.
ACCESSION   DAFEOM010000183
VERSION     DAFEOM010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.192"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            <1..>1290
                     /locus_tag="LP524_002895"
     rRNA            <1..>1290
                     /locus_tag="LP524_002895"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          344 a          286 c          392 g          268 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa
//
LOCUS       DAFEOM010000184         1258 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.193, whole genome shotgun sequence.
ACCESSION   DAFEOM010000184
VERSION     DAFEOM010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.193"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(<1..1171)
                     /locus_tag="LP524_002896"
     CDS             complement(<1..1171)
                     /locus_tag="LP524_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HBL2235875.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEF"
BASE COUNT          367 a          273 c          211 g          407 t
ORIGIN      
        1 taaattctaa atattttcgt ggagaagcca gccactcagt gtctttttcc atcaaaagtg
       61 ttccaattat ccgagtgatg gaattgatat ttggaaaaat ccgaatcact cgttctctcc
      121 ggcgaatctc ttcgttcaac cgttccaaca cattggttgt acgaatcctt cggcggatat
      181 tttcaggaaa agacaaaatt tgaatagcat cttcaaagcc gttttctaaa atgtcgcaag
      241 cagcacttaa ttttgaatca caaccatatt tttccaagaa gtgctctttt ctttcacgtg
      301 tcaattcaag ttcagaggct ttaaagatac tcttcaactc gtcttttaca tcagaagaaa
      361 ctttctttgg cagcttatca aaaatatttc tcaaaaagtg ggcttggcat ctttgccagc
      421 tcgcacctaa aaagttcttg cgaacagcct taactaaccc tttgtgtgca tcagaaatga
      481 ccagttttac accgaacaaa cctcgttgaa cgaacgattg aaaaacggta tcccaattat
      541 cttccgattc gccgtcttga acatcaaaac caagaatcgt acgtcgtcca tcagaatcaa
      601 tgccgatacc aatatataca ccttttgaga caatacgatg attttccctt actttcatat
      661 acagggcatc acacatgaaa aatggatatt ccgagccttc taggctccgg tttctccact
      721 caaaaacaaa tggatccaac tgtttcatca gatttgaaac gaatgattta gaaacagtcg
      781 caccatccgt tagcagttca actgttttag ttactttgcg agtggagaca ccagaaatga
      841 ccatttcaat catggcagcg atcaaagctt tttcgcttcg ctgatatctt tcaaagatag
      901 taggactaaa cttaccgtct ctggttcgtg gaacatgaag ttctaacgta ccaattcgag
      961 ttgtaaacga acgatcgtag taaccatttc tttgtgattg ccgttcttct gaacgttcat
     1021 aggcatttgc ctgaatatat tgatttcgtt gttcttccat cagctgatta aacatgatag
     1081 tcataagtgt ttttgcggta tcattcgcac cgctttcgtt tataattgct tgaatttctt
     1141 cgctttcaaa agtaaaatgt acttgagtca ttgatatcag tcctattcga gtatgttttc
     1201 tgcaaaaaac attgtactcc taactgagat caatggcttt tttgatgctt cactttta
//
LOCUS       DAFEOM010000185         1236 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.194, whole genome shotgun sequence.
ACCESSION   DAFEOM010000185
VERSION     DAFEOM010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.194"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            <1..104
                     /locus_tag="LP524_002897"
     CDS             <1..104
                     /locus_tag="LP524_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HBL2235876.1"
                     /translation="GTVEFPIEEGALIIATVDDPKQNVFTLEKVGVI"
     gene            104..724
                     /locus_tag="LP524_002898"
     CDS             104..724
                     /locus_tag="LP524_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235877.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            728..1177
                     /locus_tag="LP524_002899"
     CDS             728..1177
                     /locus_tag="LP524_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235878.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
BASE COUNT          446 a          200 c          280 g          310 t
ORIGIN      
        1 ttggaactgt agaattcccg attgaagaag gagccttgat catcgcaacc gttgatgatc
       61 caaagcaaaa tgtgttcact cttgagaaag tcggggtgat ctaatggcta aaactaaacg
      121 aatgatggaa acggatgaaa aaacaggtgt acaacgccaa ttctttccaa tcacacatgc
      181 ttccgcagtt cttgggttag aagaaataat agcaggagaa gcaacagttt tatctgttaa
      241 tggaaaaatt ggagccgtcg tcattactaa agaagatttg ggattagaga atgttctcac
      301 agaattaccc tatgcaagtg aagaagatga cggcattatc acggcagaaa tgtatcaaaa
      361 aattttaaac agtggagaag gtgactacgt gttgccagtt gccactatcg accgtttggg
      421 tggcataaaa gttggtgaat tattgacgat tgatgagaca gggaaagtct ctgcagtcag
      481 acaatctgat gtcaattttt cgctggagtt aaaagaaaaa ctcgattcac tgaaaaatta
      541 tactgctgga gaaaacatca ctattgatga agatggaaca attagttcta caggtggtag
      601 tgggactggt ggtgtcaatc agtcatatgt agatcaaaaa ttccaagaag ctgtaaatca
      661 agcagaaaat tacacaaacg aaagaattcc aaattttact tttgaaaaaa ttggggaggt
      721 atagagaatg acagatatcg taaaaataaa acaaagcaat gtacaggttt atccacaaac
      781 tcattggaat gctatagaag gcaaaccaac aacggtgaag ggagataaag gcgatcctgg
      841 ccaagcagca acaattacca taggaaccgt ttctagtggt tcaaccgctt ctgttacgaa
      901 tgtaggaaca tcatctgcag ctagatttaa ttttgtttta ccaaagggag ataaaggtga
      961 tcctggaata aatgccacaa ctacagctgt agctacaact acagcaaatg ggttgatgtc
     1021 gtccacagat aaaactaagt tggatggaat tgcagctgga gcacagaaaa atccaggtaa
     1081 tgctacaact acgacagcag gtttaatgtc agcaaccgat aaagtgaaac ttgatggatt
     1141 agccaatatt acatttgaga aagtggggac ggtttaatgg ctgatattgt tcaattaaaa
     1201 gaagacggag ttgctaaata cttaaaaacg catgta
//
LOCUS       DAFEOM010000186         1233 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.195, whole genome shotgun sequence.
ACCESSION   DAFEOM010000186
VERSION     DAFEOM010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.195"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            <1..104
                     /locus_tag="LP524_002900"
     CDS             <1..104
                     /locus_tag="LP524_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HBL2235879.1"
                     /translation="ETVEFPIEEGALIIATVDDPKQNVFTLEKVGVI"
     gene            104..721
                     /locus_tag="LP524_002901"
     CDS             104..721
                     /locus_tag="LP524_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235880.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            725..1174
                     /locus_tag="LP524_002902"
     CDS             725..1174
                     /locus_tag="LP524_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235881.1"
                     /translation="MTDIVKIKQSGVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
BASE COUNT          451 a          178 c          272 g          332 t
ORIGIN      
        1 ttgaaacagt agaatttcca attgaagaag gagctttgat tattgcaact gttgatgatc
       61 caaaacagaa tgttttcact cttgagaaag ttggggtgat ttgatggtta aagtaaagag
      121 aatgatggag actgatgaat ctggtgttaa acgtcaattt atgccaatta cacatgtttc
      181 tgcagtttta ggcttagaaa aaatgatatc aggacaatca aaagttcttt cggttaatgg
      241 taaatatggt gcagtcattt taacgaaagc tgatttagga ttagagaatg caattactga
      301 actaccttat gcaagcgaga caagcgatgg tattcttact gctgaaatgt ttcaaaagat
      361 cgtaaatgga gaaggtggaa catatatttt gccgattgct actcctgaac aattaggagg
      421 tataaaagtt ggggaactct tagaagttac tgaagaaggt gttttatctg caactaagca
      481 aacagacttc aatttctctg aagaattaaa gggaaaatta gaatctctaa aaatattaaa
      541 agcaggtgca aatatatcaa ttgcagaaga tggaacaatt agttctacag gtggtagtgg
      601 gactggtggt gttaatcagt catatgtaga tcaaaaattc caagaagctg taaatcaagc
      661 agaaaattac acaaacgaaa gaattccaaa ttttactttt gaaaaaatcg gggaggtata
      721 gaaaatgaca gatatcgtaa aaataaaaca aagcggtgta caggtttatc cacaaactca
      781 ttggaatgct atagaaggca aaccaacaac ggtgaaggga gataaaggcg atcctggcca
      841 agcagcaaca attaccatag gaaccgtttc tagtggttca accgcttctg ttacgaatgt
      901 aggaacatca tctgcagcta gatttaattt tgttttacca aagggagata aaggtgatcc
      961 tggaataaat gccacaacta cagctgtagc tacaactaca gcaaatgggt tgatgtcgtc
     1021 cacagataaa actaagttgg atggaattgc agctggagca cagaaaaatc caggtaatgc
     1081 tacaactacg acagcaggtt taatgtcagc aaccgataaa gtgaaacttg atggattagc
     1141 gaatattaca tttgagaaag tggggacggt ttaatggctg atattgttca attaaaagaa
     1201 gacggagttg ctaaatactt aaaaacgcat gta
//
LOCUS       DAFEOM010000187         1209 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.196, whole genome shotgun sequence.
ACCESSION   DAFEOM010000187
VERSION     DAFEOM010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.196"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            23..1201
                     /locus_tag="LP524_002903"
     CDS             23..1201
                     /locus_tag="LP524_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HBL2235882.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAV
                     YQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLIE
                     SFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFTE
                     "
BASE COUNT          444 a          225 c          200 g          340 t
ORIGIN      
        1 tcacagaaaa gaggacttcc ctatgaatga ttttactaca gaaattgtgc aaactctagt
       61 cactaaaggc gatttaaatg aattattccg ttcgcactta gaaaaagcga taaacacact
      121 cctacggact gaattaacgg cttttttaga ttacgaaaaa tatgatcgca ctggttttaa
      181 ttcaggtaat tcgagaaacg gttcttactt tcgatcaatc aaaaccgaat atggtgaatt
      241 aacattggaa atacctagag atcgtaatgg tgagtttaaa caacaaactt taccagccta
      301 caaaagaaca aacgatacat tggaaaccac tattatccat ttattcgaaa aaggtgttac
      361 gatgtctgaa attgctgatt tgatcgaaaa aatgtacggt catcactata ctccacaaac
      421 catgtccaac atgactaaag ttctgactga agaagtaaat gcctttaaat ccagagcctt
      481 aaatgataag tatgtcgcta tttttatgga cgctacttac attccactaa aacgtcaaac
      541 cgtatccaaa gaagcgattt atattgccat tggtatacga gaagacggca ctaaagaagt
      601 actgagttat gcgattgctc caactgaatc aacatacgtt tggaatgagc tgctacagga
      661 tattaactcc agaggagttc aagaagtctt gctttttatt acggacggct taaaaggcat
      721 gaaagatact atccatcaaa tttatcctaa agcaaaatat cagcattgtt gtatccatgt
      781 atctcgtaac atcgctcata aagtacgtgt caaagaccga aaagaaatct gtgatgactt
      841 taaggctgtt tatcaagcta actcaaaaga agaagcgaat accttcttat ccggcatgat
      901 tgagaaatgg aagaaaaact atcctaaagt gacgcagtca ctcatagaaa accaagactt
      961 attaactttt tatgattttc cacctagcat tcgtagaacc atttactcaa ccaatctaat
     1021 cgagtctttc aataagcaaa ttaaaagata cagccgtaga aaagagcagt ttcaaaatga
     1081 agaatcacta gaacgctttc tagtcagcat ttttgataca tacaatcaaa aatttctaaa
     1141 cagaagccat aaaggttttc aacaggtaac cgatacatta gtttcaatgt ttactgagta
     1201 actaattat
//
LOCUS       DAFEOM010000188         1193 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.197, whole genome shotgun sequence.
ACCESSION   DAFEOM010000188
VERSION     DAFEOM010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.197"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(209..465)
                     /locus_tag="LP524_002904"
                     /pseudo
     CDS             complement(209..465)
                     /locus_tag="LP524_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            complement(808..1089)
                     /locus_tag="LP524_002905"
                     /pseudo
     CDS             complement(808..1089)
                     /locus_tag="LP524_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
BASE COUNT          388 a          216 c          183 g          406 t
ORIGIN      
        1 ctagtcaaat ctctcacttc aagagtttta actagattcc agttattatt tttttgtaaa
       61 aataaacagt ctcttaatca tagacatttc ttcggtgccc tacttgaata gcggtaacaa
      121 cgaattgatt atcttctatc tctaccaata cacgtatttg ccaatcagag ctgcttctga
      181 atttcgggcg tgaattttaa acggcaggtc attccagccc accaaattca ttaaggattc
      241 ttccatgaat actgttgttt tcccacttct tatccaatgt ttatgggcaa tttcagccgt
      301 ttgacgattg tattcatctt ctagttgttc catcgaatag ttttttagaa gttcagataa
      361 agtaatccca taaaattcag ccatataaga aagccattct tttttggcgt cacttacacg
      421 aaccgtaatc atagccctat aattgccttt cttaaagaat ctcatgcatt tcctgatgtg
      481 attttagaaa aatttacttg ctaaaaaact gtgaaaatgt aggagctagc atccttgtag
      541 taaatgatcg agctctttat tatacattaa aaaagttcag aaattttgat gaagtagcca
      601 actacttttt catgagtaaa gcaacactag aaaaagaatc ctccagtact tagaatttac
      661 tggaggatgt acttttgaag aagcctatag gttatttaaa aaatattact acaggaagga
      721 acgagaatta cttcgttact tataatagaa cttagtctta tatagaaggc atagctcaca
      781 ttagcttatt tgtcctataa aaataaatta cggtatttta gaaaagctat tttctctttt
      841 ttcaacgatc ttcagcaatt tttctttatc gtcaggtaac tcaagttcat gaattctagc
      901 atgacaattg ggacataacg cgatacagtt ttcaatcaca tcttttccac cttgcgacaa
      961 atattctata tgatgacaat gtaaataagg tacaccgtct tttataaatg gccctttttc
     1021 ttggcaaagt tgacaaatac cttttgcaag tcgtttaaca tattcagata cgaagggatt
     1081 acgtttatac ccgtggtgct agttaatttt ttcctagagg aaaatgtcta aacatgttgg
     1141 taaatcaaca aaaaaacgca tatagtagtc tcaaaactcc caagaaatga gga
//
LOCUS       DAFEOM010000189         1193 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.198, whole genome shotgun sequence.
ACCESSION   DAFEOM010000189
VERSION     DAFEOM010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.198"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            <1..1076
                     /locus_tag="LP524_002906"
     CDS             <1..1076
                     /locus_tag="LP524_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBL2235883.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DAFEOM010000190         1173 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.199, whole genome shotgun sequence.
ACCESSION   DAFEOM010000190
VERSION     DAFEOM010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.199"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            31..291
                     /locus_tag="LP524_002907"
     CDS             31..291
                     /locus_tag="LP524_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235884.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(653..892)
                     /locus_tag="LP524_002908"
     CDS             complement(653..892)
                     /locus_tag="LP524_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235885.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          416 a          164 c          218 g          375 t
ORIGIN      
        1 taatggaaaa acattaagga tggatgaaat atgaataacg aaactatcat tttaggaaaa
       61 aaatatattt gccaacctat tggcttgaaa caaaaagtgg ttggagaagt aatcagtaaa
      121 ttagaaaatt gtgttgtgat ctgcgtagac gaatatcatg acgtcgatcg caatgaaata
      181 ttagaaaaat gcggaaaagt agtggcaaaa tatactcacg tttatgagtt ggaaacaaca
      241 gaatatctat ataactatag caacaaatat gaacatactt tagtttcata aagatttaaa
      301 taaagtgcct tcaaaagcga gaagaccaag cttttgaagg cactttattt agttggcagt
      361 gattgttcat gatttttgat ttattcaaaa ggttcgccta gagaatctgt ctctgtaacg
      421 aggcctggtg gagaaactcg ataaaaacct atactgccag aactgtccta ttttcggata
      481 gacaaactag tagttttaaa aagatcatcc gatccaatca tttcaaaaaa tgcaaaactg
      541 atatcaggat catttagctc aggcttgtta tcgtaaagca tttgtaaagg ttgcatttag
      601 ttaggagtaa acgcgaaact atcatcatta tttgctgaag aagatgaaga agctaattca
      661 gagtttgttt gaaataaagg agacgtgctg acagtttctt taccctgttt tttgcagagg
      721 tagaaacttt tatatcggaa gtttctaccg gagaattttt ctcatttgta gaaattgttg
      781 aatcgatact attcgaaatt tgcgtatctg aaggtatgtt ctttgatgca gaagtcccac
      841 aagcagtaag taacaataaa tttgataaac tgataaacag aattcttttc attatgtaca
      901 cactccttat agtatcgttt agcatacacc tatgctcagt attaggcaaa atcaatgcaa
      961 aactatcaag tcttttatag tatctattag tcttagtaat acatatatgg agagggtgat
     1021 atcccagttc aaaccagata cattggttac catctgtatt aacgattatg cctctaaacg
     1081 ctttttttat gaaaaaattt ataagtgaaa acgtaggtat gatattatct agaggtaatt
     1141 tttttgggta tttaaatagt tgctaggagg ata
//
LOCUS       DAFEOM010000191         1146 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.200, whole genome shotgun sequence.
ACCESSION   DAFEOM010000191
VERSION     DAFEOM010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.200"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            <1..354
                     /locus_tag="LP524_002909"
     CDS             <1..354
                     /locus_tag="LP524_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBL2235886.1"
                     /translation="TQAAYAFLKRLVKQFDEPKVVVTDKASSITSAFKKLKEYGFYQG
                     TEHRTIKYLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFG
                     FSVSTEIKVLMGITA"
BASE COUNT          426 a          177 c          181 g          362 t
ORIGIN      
        1 acacaagcag cgtatgcttt tcttaagcgg ttagtgaagc agtttgatga accgaaggtt
       61 gtagtcacag ataaagcctc ctctattaca agtgccttta agaaactaaa agaatacggc
      121 ttttatcaag ggacagaaca tcgtaccatt aaatacctga ataatttgat tgaacaagac
      181 catcgaccta ttaaacgacg aaataaattt tatcaaagtc tccgtacagc ctcttccacg
      241 attaagggca tggagaccat tcgaggaata tataaaaaga accgaagaaa tggaacgctc
      301 ttcggctttt cggtgtctac tgaaatcaag gtattaatgg gaataacagc ctaagatatt
      361 tggagttcag agagggcgcg tttgattttc aaacttcgca acagaaccaa agaaggtatc
      421 aagagtcaca gtgaatttat tccgaaaagt gaaaagagca aatataaaat ttaataaaaa
      481 tatagaacta aataggaaaa cgtagacgta attttctacg ttttcctttt catatatatt
      541 aaatattacg gaaaattata taaactaatt tagtgttttg tttgtaattc ctaattttgc
      601 tccactttta gataattttt aggtctattt aactcatcag acattctatg ctcattttct
      661 ccaaatttgt ttcaactctt tcgtacttaa ttttgcgtat ttgaaaattc ttcttttaga
      721 gtacttttat tacgatacta gtatgatata tatctatagc aaacagttaa aatcttccta
      781 aaataagcga atagcattcc taaaacctat aaaattttta gatgaagcga caagtggaca
      841 attaataaac tacttgaaca tgaaattatg gatcaccttt caaatttaga aaatggaatt
      901 cttatactta ttgcacaaca tttattgatt gcaaaattat gtgacaaaaa gaactagtag
      961 aagaaggaat aatggcttga taataaagta ctacctatta aaggtagtac tttatttaat
     1021 ttagtataaa ttaacatact aacccgtggt gctagttaat tttttcctag aggaaaatgt
     1081 ctaaacatgt tggtaaatca acaaaaaaac gcatatagta gtctcaaaac tcccaagaaa
     1141 tgagga
//
LOCUS       DAFEOM010000192         1124 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.201, whole genome shotgun sequence.
ACCESSION   DAFEOM010000192
VERSION     DAFEOM010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.201"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(136..1122)
                     /locus_tag="LP524_002910"
     CDS             complement(136..1122)
                     /locus_tag="LP524_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HBL2235887.1"
                     /translation="MLTALFLLPINGFAYTINNEFNLGANEGSSQVANNQYILLHETA
                     NETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIE
                     LQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHT
                     DPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAH
                     IDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRSDVNQAYNTS
                     GNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          312 a          223 c          208 g          381 t
ORIGIN      
        1 aaacctgtta tcgcatagct tggagtgtga ctattatggg tgatagtgaa gatatgcgat
       61 aacatcacta ttttatcgaa tgatttttat agttgtcaat atagttatgt aatgctcctc
      121 aacgaggagc tatttttatc gtttaggaat atttaaatac caacgtttgt catggaaatc
      181 ttgcgcaccg cctttagtgt ttccctctgg atcattcgtt gcccgcatca tgacatagac
      241 tttcttatta gggaagttac gcatgttaaa agatacatga tagccaacat ttccagaagt
      301 attataagct tgatttacat ctgatctata aattccatca gctcttactc gagctaattc
      361 tttcccagta ttgtaatcca taatgaagat atactcgtat ttatagttag caatgtgcca
      421 tccagctaca tgcaaatttg cattttcgat ttctccaaac tgatcaatgt gggcgtaatt
      481 cgttccatct gtcagtgtag gattagctgc accagctcgt gttggatcaa tgacaggctt
      541 gttttcagaa gttgttggat tttcatcggt aaatccatga gctaaatcat atgctaattt
      601 ttctttactt acgcccattt cagaaagata accgtaagga tctgtatgat cgccccagat
      661 gttttgtgtt acccataaat gcgatttgat tcccggttgg ttataaggag tgtccaacgt
      721 taatggaata ccatatttca ttgctgaatc tctagccaat tcaacgtatg ccttgtagtt
      781 tttctcaaac gttgctttat catgtgtgtg ttgtaactca atctgcacag gactgttggc
      841 attagcatac gaaccagcac cgtactgtac ataaccaggt tgaccgactt gataaacaat
      901 tccgccgtct cccacaatat aagcagtata agcgctagtc catgaacgtt gcatatactg
      961 cgcttcattg cgtcctgttg ctgtttcatt agccgtttca tgcagtaaaa tatactgatt
     1021 atttgctact tgtgagctac cttcatttgc gcccaaatta aattcattgt tgatagtata
     1081 ggcaaatccg ttaattggca ataaaaaaag agccgttaac aggc
//
LOCUS       DAFEOM010000193         1058 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.202, whole genome shotgun sequence.
ACCESSION   DAFEOM010000193
VERSION     DAFEOM010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.202"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(36..992)
                     /locus_tag="LP524_002911"
     CDS             complement(36..992)
                     /locus_tag="LP524_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235888.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgt
//
LOCUS       DAFEOM010000194         1052 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.203, whole genome shotgun sequence.
ACCESSION   DAFEOM010000194
VERSION     DAFEOM010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.203"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="LP524_002912"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="LP524_002912"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBL2235889.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          377 a          132 c          182 g          361 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt tt
//
LOCUS       DAFEOM010000195         1002 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.204, whole genome shotgun sequence.
ACCESSION   DAFEOM010000195
VERSION     DAFEOM010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.204"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            129..>1002
                     /locus_tag="LP524_002913"
     CDS             129..>1002
                     /locus_tag="LP524_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBL2235890.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDF"
BASE COUNT          360 a          153 c          189 g          300 t
ORIGIN      
        1 aatatcccat attttaaaat aaaagatagc gtaaaatatt ttgtgtaaat agaaatttag
       61 ggtagaaaaa aaagtccatt ctgtaaaatt aaagttacca cacagaaatt ttataggagg
      121 actttctcat gactaaattt actatagaaa ttatgcaaac actactcaat aaaggggatt
      181 tagatgaatt atttcaggat catttagagc gagcgattaa ttcacttcta caagcagagc
      241 taactgcttt tttagattac gaaaaatatg atcgaaatgg attcaattca ggaaattccc
      301 gtaacgggaa ttattcacgt acatttaaaa cagaatatgg cgagttgaat ttaactattc
      361 ctagagatcg gaatggcgaa ttttctcaac aaacactacc agcatataag cgaaccaatg
      421 attcattaga aacgaccatt atccaattat ttcaaaaagg cattactatg gctgaaatct
      481 ccaaattaat tgaaaaaatg tatggtcacc actatacgcc acaaacaatc tccaacatga
      541 gtaaattggt ggctgaagat gttttagctt ttaaagaaag aacgttagaa gccaattatt
      601 ccgttatatt tatggatgcg acgcatatcc ctgtgaagcg acagactgtt tcgaaggaag
      661 cagtgtatat tacgattggt attcgtttgg atggaaccaa agaagttctt gggtttacta
      721 ttgccccaac tgagtctgct tatatttgga aagaagttct tcaagatctt agaaaacgtg
      781 ggttagaaga agttttatta gtagtgacag acggattaag ctgtattgaa gaaagtatcc
      841 atagtgtgta tccgaatgcc caatttcaac aatgttgtgt gcatgtatct agaaatatcg
      901 ctcataaagt tcgtgttcga ggtcgaaaag aaatttgtga ggatttcaaa ttggtttacc
      961 aagcgaattc aaaagaagag gcattggatc acatcgactt ta
//
LOCUS       DAFEOM010000196         1002 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.205, whole genome shotgun sequence.
ACCESSION   DAFEOM010000196
VERSION     DAFEOM010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.205"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            52..307
                     /locus_tag="LP524_002914"
                     /pseudo
     CDS             52..307
                     /locus_tag="LP524_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752209.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            371..1002
                     /locus_tag="LP524_002915"
                     /pseudo
     CDS             371..1002
                     /locus_tag="LP524_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          344 a          181 c          183 g          294 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaaaa
      301 tataggctct ataatttatg ggactgatga atcaaaattg attcttcagt cccattgtta
      361 ttttaggtat atgattcgta cattgaaaga gttgaaaaac tatcttccga agccgtcaaa
      421 aaggaccctg ccaaaagtat tgatggtgga tgaatttcgt tcgcacgctt caattgtgga
      481 taaaatgagc tttatttgtg cggatggcga gactggaaaa ctcattgata ttttgcctac
      541 acgaaaattg tctcgattaa cacactattt tagtcgctgt gcaaatcctg aagcggtgga
      601 atatttagtg acagatatga acgccgccta cttccaactg acaaaacgtg tcttaccaaa
      661 tgcgaaagtc gtcattgatt gttttcatat catcaaacat ttgaatcagg cgttcaatga
      721 ttttcgtgta caagaaatga aaagattaca ccagttaggg caacgacggg aagccgagaa
      781 agtgaaaaag aactggcgct ttcttctaaa aaaccgtcaa aaaatcaatc actatgatta
      841 caaaacatgg cgcagttttc gtgcacctaa atacccgcta ctcactgaag caatgatgat
      901 tgatcgattg ctggcatttt caacgccttt aaaggacgcc tatacttttt tccatgagct
      961 gaccgaagcc tttagagaca aggaatcaga cttatttttt ac
//
LOCUS       DAFEOM010000197          991 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.206, whole genome shotgun sequence.
ACCESSION   DAFEOM010000197
VERSION     DAFEOM010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.206"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            54..>991
                     /locus_tag="LP524_002916"
     CDS             54..>991
                     /locus_tag="LP524_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL2235891.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTK"
BASE COUNT          355 a          180 c          189 g          267 t
ORIGIN      
        1 ttgtgctaca ctcaaaatag ttccgacgaa agaattataa ggagtgagta caaatgacct
       61 ataaccatct tacaccaact gaactcgtaa tgatagaagc ttatttcaat caatcacaac
      121 ctgtttcaaa agtagccaat ctgttacaac gttctcgaca aactatttat aaggtctatc
      181 gttttcttaa atctggtgga agcgcaatga gctactataa acaatataag aaaaacaaac
      241 gtaactgcgg aagacatccg attgttttac ctgatgagca acaggaatac attcaaaaaa
      301 aggttgttca agggtgggct cctgatgtcc ttattggtcg agcagagttt cctattgctt
      361 gttcggtacg cacactgtat cgaatgttta aagataaaac ctttgacact cacgatttac
      421 caatgaaggg aaaaagaaaa ccaaacggtc ataaagaaaa aagaggaaaa caagggtttc
      481 gccggtcaat ccgagacaga aagacagact atacccagtt tgatcatgaa tttggtcacc
      541 tggaaggtga cactatcgtg ggtggaaaac ataaaagcgc ggtcatcaca ttagttgaac
      601 ggctatctaa agtcatcatt accttgaaac ccgaaggcag acaggctaaa gacattgaaa
      661 ataggctgaa tcaatggttt cgttgtgtcc caaaaaactt atttaaatcc atcacatttg
      721 attgtggaaa agaattctca aattggaaaa agattagtaa taccaacgat atttcaattt
      781 attttgcgga tccaggaaca ccttctcaac gaggattgaa tgaacattca aatggcttat
      841 tacgtaaaga tggtttgtat aaagctatgg attttaatgg tgtatctgaa acctttatcc
      901 aatcggtggc atcgaaacga aatcatattc ctagaaaatc attacactat cggacaccaa
      961 tggaagtatt tttgagttac gtcacaaaag a
//
LOCUS       DAFEOM010000198          964 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.207, whole genome shotgun sequence.
ACCESSION   DAFEOM010000198
VERSION     DAFEOM010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.207"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            234..665
                     /locus_tag="LP524_002917"
                     /pseudo
     CDS             234..665
                     /locus_tag="LP524_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(722..>964)
                     /locus_tag="LP524_002918"
     CDS             complement(722..>964)
                     /locus_tag="LP524_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBL2235892.1"
                     /translation="SVGLYTKTEHRTVKYLNNLIEQDHRPIKRRNKFYQSLRTASSTI
                     KGMETIRGIYKKNRRNGTLFGFSVSTEIKVLMGITA"
BASE COUNT          319 a          141 c          159 g          345 t
ORIGIN      
        1 cttttaataa atactaccta aataaggtag tatttatttt tgtgttttaa aagaggaagt
       61 ccaatatatc tatgggaaac ttcttaaata ttatgttggt acatgaaaat atacatccct
      121 gctagactat ttacaacatt ttactttgta cgtattcttg ttttaaaagg aatgggataa
      181 aatataggta caataactat ttgatgctaa atataattaa tggaggagag atagtgaaca
      241 tacttaatga aaaaagatat gtatggtttg ctggtaaaaa atatattact actttcaaaa
      301 gattatccga gttaatgcag cagactgtta aaagtgctat gcctgaagga tacaagttta
      361 attttgttta atcttcagta tctactgaaa gtatctattt ttcaattctt tctgagagta
      421 ggctttttca ttttagaata agttctcatg taaagaaaac tgatgttgat atatatacgt
      481 tccgtacaaa taattataaa aatttaaatg atttgagtag aagtatcagt gaatatctca
      541 aggggaatag ctatctcaag attcgatttt cacatatatt aattttacta ccatatttga
      601 atgagaattt caccctaatt caagtagata agtctttcat tgcctcaggt aatcaaggaa
      661 atatgaaggt tctgttgcga agtttgaaaa tcaaacgcgc cctctctgaa ctccaaatat
      721 cttaggctgt tattcccatt aataccttga tttcagtaga caccgaaaag ccgaagagcg
      781 ttccatttct tcggttcttt ttatatattc ctcgaatggt ctccatgccc ttaatcgtgg
      841 aagaggctgt acggagactt tgataaaatt tatttcgtcg tttaataggt cgatggtctt
      901 gttctattaa attgttaaga tacttcacag ttcggtgctc tgtcttagta tataaaccca
      961 cact
//
LOCUS       DAFEOM010000199          943 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.208, whole genome shotgun sequence.
ACCESSION   DAFEOM010000199
VERSION     DAFEOM010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.208"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            <1..258
                     /locus_tag="LP524_002919"
     CDS             <1..258
                     /locus_tag="LP524_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235893.1"
                     /translation="KGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            260..397
                     /locus_tag="LP524_002920"
     CDS             260..397
                     /locus_tag="LP524_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBL2235894.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            434..727
                     /locus_tag="LP524_002921"
     CDS             434..727
                     /locus_tag="LP524_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235895.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            724..>943
                     /locus_tag="LP524_002922"
     CDS             724..>943
                     /locus_tag="LP524_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBL2235896.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGN"
BASE COUNT          350 a          134 c          201 g          258 t
ORIGIN      
        1 aaagggaatg ctgttgatag cgtagatgaa ttggctaata ttaaggcatt atacgaaacg
       61 ctgaaggcag aaaatgatga attgaaacag ctaaattcta aagcaatgct aaacaatgta
      121 gcaatcaagc aagaaaatgt cttattgaaa gaaaagtcag aaagtttagc gcagttgaat
      181 tcaaaaacga tgcttgcttc tgtacaaaat accaaggaaa tcgaagaaat caaaaaacaa
      241 ttacaaggtg ggaagtaaca tgtattcata tgaagatatc aaactaatgt atgactgggg
      301 ctgtttcact aacgaacaag taatggtttt cgttccatta tgcattacag acgaagaagc
      361 agataaaatc attaataaag ataagagcgc atcttaattg atgcgttttt atttaaggta
      421 aaggagttgt cacatgatta atttagggga atggggaatg atagcaggat caataaccgc
      481 tatcgtttct ttgattttat tagtaattag accgattgct gcatctttct cgaaaattac
      541 tgagactctt tcaaaagtaa gccacaattt agatttgctg actaaagatt tagaatcgag
      601 caaatcagat cgattgatga ttcatgaaga actaaagaaa cacgatgaaa gattagatac
      661 acatacagaa aaattggtgg aacacacgca acaaatcaaa actttgttta gggagagaag
      721 aaaatgaata ataaaacgtt cgaagtacta aaatggttcg cactggtaat tattcccgca
      781 ctagctactt tcgtggggtt agttggtaaa gcgctcaatt ggcagtacac agatatctgt
      841 gttgtcatca ttactggttt tggcgcgttt ttagggagtg tgttgggtgt atcaaatcga
      901 acctacaaaa tgttctcggc tgaaagcgaa gaaggaggaa aca
//
LOCUS       DAFEOM010000200          925 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.209, whole genome shotgun sequence.
ACCESSION   DAFEOM010000200
VERSION     DAFEOM010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.209"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            53..>925
                     /locus_tag="LP524_002923"
     CDS             53..>925
                     /locus_tag="LP524_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL2235897.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNN"
BASE COUNT          320 a          161 c          173 g          271 t
ORIGIN      
        1 tgtgctacac tcaaaatagt tccgaccaaa gaattataag gagtgagtac aaatgaccta
       61 tacccatctt acaacggatg aacttgtaat aatagagtct tatttcaaaa tgaatcaatc
      121 tgttgctaaa actgcccatt gcttgaatcg ttcaagacaa acgatccata aagtatatct
      181 gttcttcaag caaggaaaat cagccttaga atattatcaa cagtataaga aaaacaaatc
      241 aaactgtggt agacgtccgc ttgttttacc cgaggaacaa tcagaatata ttcaaagaaa
      301 ggttgttcaa ggatggacac ccgatgtgat tgttggtcgt gcagcgtttc ctattagttg
      361 ttctgctcgt accatttatc gtatgttcaa aaaggggcta tttgattctt ctgacttacc
      421 gatgaaaggc aagcgtaaac cgaatggaca tcaagaaaga cgtggaaaac aaactttccg
      481 ccgctctatt catgaacgtg aaaaggatta tagccaattc tcaaatgagt ttggtcacct
      541 tgaaggtgac actatcgtag gtctgaaaca taaaagtgct gtaattacct tagttgaacg
      601 attatcaaaa gttatcatca cattgaaacc gtgtggtaga caagcgattg atattgaaaa
      661 aaaattaaat caatggtttg aatctgtacc gaaaaaccta ttcaaatcca tcacttttga
      721 ttgtggaaag gaattttcaa attggaaaca gatcagtaat gtcaatgata ttgccattta
      781 tttcgctgat ccaggaacgc cgtctcaaag aggcctaaac gagaattcta acggattgtt
      841 acgtagagat ggtttattga aatctatgga tttcaattca gtagatgaat tttttattca
      901 atctgtcgca tctaaacgaa ataat
//
LOCUS       DAFEOM010000201          818 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.210, whole genome shotgun sequence.
ACCESSION   DAFEOM010000201
VERSION     DAFEOM010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.210"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            1..797
                     /locus_tag="LP524_002924"
                     /pseudo
     CDS             1..797
                     /locus_tag="LP524_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
BASE COUNT          286 a          153 c          148 g          231 t
ORIGIN      
        1 actacacgcc ccaaaccatg tccaacatga ctaaagtgct gactgaagaa gtaaatgcat
       61 ttaaagttag aactctaaat gataagtatg tcgctatttt catggatgct acttatattc
      121 ctttaaaacg gcaaaccgtc tccaaagaag caatctatat tgccattggt atacgagaag
      181 acggcactaa agaagtacta agttatgcga ttgctccgac tgaatcgacc tacgtttgga
      241 atgagttgtt acaggacatt tactccagag gggttcatga tgtcttactg tttattaccg
      301 atggcttaaa aggcatgaaa gatacgattc accagattta tcctaaagct aaataccaac
      361 attgctgtgt tcacgtttcc cgtaatattg ctcataaagt acgtgtcaaa gatcgaaaag
      421 aaatctgtga tgattttaag gctgtttatc aagctagctc aaaggaagag gcaaatacct
      481 ttttagggag tatgattgag aagtagcaaa aaacttatcc taaagtgacg cagtcactga
      541 taaaaaatca agatttattg actttttatg agtttccgcc tggtatccgc cgaagcattt
      601 actcaactaa tttaatcgaa tctttcaaga aacaaatcaa gaaatacagc cgcagaaaag
      661 agcagtttca aaatgaagaa tcactagagc gtttcctagt atccatcttt gatacataca
      721 atcaaaaatt cttaaataga agccataaag gcttccaaca agtgacggat acattagctt
      781 caatgtttac tgagtaaccc attattttgc agaaggac
//
LOCUS       DAFEOM010000202          791 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.211, whole genome shotgun sequence.
ACCESSION   DAFEOM010000202
VERSION     DAFEOM010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.211"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            <1..782
                     /locus_tag="LP524_002925"
     CDS             <1..782
                     /locus_tag="LP524_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL2235898.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          340 a          107 c          102 g          242 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgcccc t
//
LOCUS       DAFEOM010000203          763 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.212, whole genome shotgun sequence.
ACCESSION   DAFEOM010000203
VERSION     DAFEOM010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.212"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(237..716)
                     /locus_tag="LP524_002926"
     CDS             complement(237..716)
                     /locus_tag="LP524_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235899.1"
                     /translation="MCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNSL
                     DFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKGKKIKKCDKCFESAVVKFPQFKLNP
                     AASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQISSNLEDIKKAFKIKTIGTAPNFK"
BASE COUNT          261 a          101 c           85 g          316 t
ORIGIN      
        1 aacaataaat gaagactttt ttattgttaa ttgtttattt ttctattatt agatatgaaa
       61 atcgcattta gataaaatag aaaaatatga atatatttta aatattaaga tgagagtcat
      121 taacttatga tagtagtcaa tttgcttttc actaattaca aaatatgtga tataggaagt
      181 ttagttattg ttctcataat gccatcctgt ttttagatta tctagtaatt tttttgttat
      241 ttaaaattag gagcagtgcc aattgtttta attttaaagg ctttttttat atcttctaaa
      301 ttagaagata tttgtatatc aatatggaat atattacctt ttttgtattt ttttatgtta
      361 tctttagcta aaacatcaaa tacatgtcct tgaaattgta tcgaggcagc tggatttagt
      421 ttaaattgag gaaactttac cacagctgat tcaaaacact tgtcacattt ttttattttt
      481 ttacctttgt aaaaatattc gattgaatat tctatttggc acgactcact tggcagtata
      541 tgtatgtcaa tatttttcat ctgtttcata ggtatttcaa aatctaaact attaaaaaca
      601 tataattccc tgtaattatt tacatctaac tctactaaat ttatctttgg aacaatagat
      661 cctccaccca ttccaccata ataaaaaaaa tcttgttctc tatcaaaata gcacatatta
      721 agctcagttt gtttatcatc ctcaataatt ttataaatca aac
//
LOCUS       DAFEOM010000204          742 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.213, whole genome shotgun sequence.
ACCESSION   DAFEOM010000204
VERSION     DAFEOM010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.213"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            176..379
                     /locus_tag="LP524_002927"
     CDS             176..379
                     /locus_tag="LP524_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HBL2235900.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
     gene            379..729
                     /locus_tag="LP524_002928"
     CDS             379..729
                     /locus_tag="LP524_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBL2235901.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFIDNDEKLLIELGDISKKAGINSKYKANFTD
                     KSQFYSYSETMPQR"
BASE COUNT          299 a           80 c          119 g          244 t
ORIGIN      
        1 cttccttttt tgtatggatt ttttcattta cacaaaatat tttacactct cgtcaatcat
       61 tgttatataa taaaatgtta tataataaaa ttactaatga tatttaatat tacaagcaat
      121 cttaagaaat tcgaaaagga aaaaatgatt atgaaaaaga aagaaaaaga aagtattgaa
      181 acattgtgtt attctaggaa tattaggaac ttgtctagct ggcattggta caggaataga
      241 cgttgacgca gctacttact atggaaatgg tctttattgt aacaaagaaa aatgttgggt
      301 aaattgggga caatcatggt cagaagggtt aaaacgttgg ggagataatc tttttggtag
      361 ttttagtgga ggacgataat gacttcaata aacattgatc acacaaaaaa agtcatacat
      421 gaattataca atagtttaat gacacgacct aatcagacga aagcattatt agatattact
      481 gatgttttat ttcaggtata taaaaaaatt gatacagcaa aatatcctga aattcttgta
      541 aataaattag ttaattatat ttacatcgtg ggttttgata ataaaatacg ctttattgat
      601 aatgatgaaa aattattaat agaattaggg gatatttcta aaaaagctgg aattaatagt
      661 aaatacaaag caaactttac agataaatcg caattttata gttattcaga aacaatgcct
      721 caacgataaa atatagtata ct
//
LOCUS       DAFEOM010000205          731 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.214, whole genome shotgun sequence.
ACCESSION   DAFEOM010000205
VERSION     DAFEOM010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.214"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="LP524_002929"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="LP524_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBL2235902.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..675)
                     /locus_tag="LP524_002930"
     CDS             complement(481..675)
                     /locus_tag="LP524_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235903.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          220 a          156 c          122 g          233 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc
      661 atttaattta tacatagaaa acctccagac ttcttgatag ctcaagtata tcttgaggtg
      721 catttgttag t
//
LOCUS       DAFEOM010000206          694 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.215, whole genome shotgun sequence.
ACCESSION   DAFEOM010000206
VERSION     DAFEOM010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.215"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
BASE COUNT          247 a          116 c           84 g          247 t
ORIGIN      
        1 ccaaattact tcatcaaaac attgatataa tttttattat atcagtattt attcttgata
       61 cataaatgag aaaataaata ctttaaatct attgactaca agtagtcatt aagtcttcat
      121 aagatgtaca atatgacatt aaaaaaataa aagcttacga ccatccgtta aatctgacac
      181 taaataatgg actgctttct tttgtattag caggttacgt tgactatgaa tattcaccag
      241 attatttgga gaaacctctt caacacgaga acaatgatat taattttaga tttgtctacg
      301 agtcaaaaag taaaacatgg ttagttgatc aattaagcac gataaacaca agcttccatg
      361 aattacctaa acctgaaaat gaagttgaga tggtcattaa aatcttctaa aaaagaagcg
      421 ttgaagggga aatattcctt tcaacgctct tttttatatg cttagaaaca taaataaatt
      481 gtatactttc tttttagccc ctattacttt tcacgaaaaa tatctttttt tcgacgctag
      541 cgtttctcta cttctaatac tgtacacagt ttttgtatta ttgaaaaatt actcattatt
      601 tagcattaat ccacatcgtt ttatcaatcg accttcatac taaactactt taaagtcatc
      661 ttccttctcg tttttcatta atggaatggc aaca
//
LOCUS       DAFEOM010000207          677 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.216, whole genome shotgun sequence.
ACCESSION   DAFEOM010000207
VERSION     DAFEOM010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.216"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            219..>677
                     /locus_tag="LP524_002931"
     CDS             219..>677
                     /locus_tag="LP524_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBL2235904.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRV"
BASE COUNT          232 a          104 c          149 g          192 t
ORIGIN      
        1 tttctgaagt acatccgcaa ctgtccatac tctgatgttt tatatctttt ctaaaagttc
       61 gctagataga gttctatatt agagatgtaa agagttttgg tgaagaggtt tgtgagtatg
      121 gcttttataa ccagggtgca atgagaagca ctaacagcag ctagcttaag aacgcatagg
      181 atatactttt tatggaagtc acagaggagg aataaaattt gaacaggatt aacagagtaa
      241 cctctattct tattcagctg caatcaaaaa aaataattcc tgccaaagaa attgcacagc
      301 gatttaatat aagcttaagg acagtttaca gagatatccg gacacttgaa gaagccggaa
      361 taccaattgg atctgaggcc ggaaaaggat attttcttgt agagggcttc ctacttccgc
      421 cggtaatgtt tacagcggcg gaagtgggtg cattgattac agcagggaaa tttttaaatt
      481 gccatggaga tgaatcattt ataaaggatt ttgattcagc tatgtataaa atcaaatcta
      541 ttttgaagca tggtgaaaaa aactatgcac aggagttgga gaacagtatt aacgtgtaca
      601 gcacatctgg acagaaaaat acattggcgg ataacgttat tgcagcaatt cagactgcaa
      661 tctgcaataa aagggta
//
LOCUS       DAFEOM010000208          670 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.217, whole genome shotgun sequence.
ACCESSION   DAFEOM010000208
VERSION     DAFEOM010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.217"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(<1..>670)
                     /locus_tag="LP524_002932"
     rRNA            complement(<1..>670)
                     /locus_tag="LP524_002932"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          134 a          213 c          147 g          176 t
ORIGIN      
        1 gtttcaacct tgcggtcgta ctccccaggc ggagtgctta atgcgttagc tgcagcactg
       61 aagggcggaa accctccaac acttagcact catcgtttac ggcgtggact accagggtat
      121 ctaatcctgt ttgctcccca cgctttcgag cctcagcgtc agttacagac cagagagccg
      181 ccttcgccac tggtgttcct ccatatatct acgcatttca ccgctacaca tggaattcca
      241 ctctcctctt ctgcactcaa gtctcccagt ttccaatgac cctccccggt tgagccgggg
      301 gctttcacat cagacttaag aaaccgcctg cgctcgcttt acgcccaata aatccggaca
      361 acgcttgcca cctacgtatt accgcggctg ctggcacgta gttagccgtg gctttctggt
      421 tagataccgt caagggatga acagttactc tcatccttgt tcttctctaa caacagagtt
      481 ttacgatccg aaaaccttct tcactcacgc ggcgttgctc ggtcagactt tcgtccattg
      541 ccgaagattc cctactgctg cctcccgtag gagtttgggc cgtgtctcag tcccaatgtg
      601 gccgatcacc ctctcaggtc ggctatgcat cgtggccttg gtgagccgtt acctcaccaa
      661 ctagctaatg
//
LOCUS       DAFEOM010000209          598 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.218, whole genome shotgun sequence.
ACCESSION   DAFEOM010000209
VERSION     DAFEOM010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.218"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
BASE COUNT          244 a           95 c           81 g          178 t
ORIGIN      
        1 cttttttgta tggatttttt catttacaca aaatatttta tactctcttt tgttactatg
       61 aataacataa taagaaagta aagtggattg atttgagatc tttttatact tcaaaaaaga
      121 taacacccaa ctttcaaaaa tatgttttgg aagttgggac aaacgagtta atacatttta
      181 atacattatt ttttattcaa aaaaaatagc acaatggcaa agatcgtaag agagatcata
      241 agccccaatg aatgactaaa aagaaaagca gaatcacatg ccatatcatc ccatatttct
      301 tcaagaaaat aaataagcgg gaaaaaggga aggaatagta gaacatcgat ccagcaccat
      361 ttttttcttc ttttgaaata acataaaaca ataaacatga acgtgcaggg aagtaaaaac
      421 aaagctttac tcataactaa caaaatacca gctcctttta tttttttctc ccccacattc
      481 aaagagtaac aatggtatgt acttaataat agaaaaatgg atcaacttgt atgaataaca
      541 gctgaaaaga tctgcaccaa ataaaaaact tccagccaaa actgactgga agtttatt
//
LOCUS       DAFEOM010000210          587 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.219, whole genome shotgun sequence.
ACCESSION   DAFEOM010000210
VERSION     DAFEOM010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.219"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
BASE COUNT          191 a           83 c           83 g          230 t
ORIGIN      
        1 aattatagag ccattttttc ttgatgtgat atccttgtct tttttgtaat gtttttaact
       61 tattgctttt gcaaaattta tttacctgtt ttctggatca acgatagtgt acattatgct
      121 atccttgttt ggattttagg acgtttaaag aggcactttt ttacataact taatccatag
      181 accttttaag catgctgtga gataataata aagaagagtt taaagcgcac cccaaaccac
      241 ttccccataa gtgtattacg ctttaaactc ttttatattt gaattcacta agaagcatac
      301 catatttttg aaaaaaagtg agaaaaaagg cttataatta ggatttagag taattaatta
      361 gatgtttatt ttataggact gatactatag attacaaata gtatcgtatt tcgcaatctt
      421 aaaattatct tcttactgtg aataagataa taaaatttgt atttgtcctt tttaggtaac
      481 ttattttaat ctatatacct ttctggatca acgaaagtat actttatata gtcataacgc
      541 cgatggtaac tatgcactgt aacgataatg ctgctaaaat atctttt
//
LOCUS       DAFEOM010000211          578 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.220, whole genome shotgun sequence.
ACCESSION   DAFEOM010000211
VERSION     DAFEOM010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.220"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            126..413
                     /locus_tag="LP524_002933"
                     /pseudo
     CDS             126..413
                     /locus_tag="LP524_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            427..>578
                     /locus_tag="LP524_002934"
     CDS             427..>578
                     /locus_tag="LP524_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235905.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWV"
BASE COUNT          200 a           91 c          122 g          165 t
ORIGIN      
        1 cattggtgca gacagatgga gaccaagcca ttgcaggaca taagaatttt tcaggttctg
       61 taacaatcaa taataaacgt gttttgacga ctgatgataa cagatatgaa gtggtaaatc
      121 tggttgtcac taacggtaat acagggacag caaagcttta tcgtgaagga aaaacagttt
      181 ctatatattt tgtggcttta aacggaaaaa gtagtggcgg gaatgattcg gttattttaa
      241 ctgttccaga aggctatcgg acaccaatta gttttgaaca actggttggg tcaatagacc
      301 gttctacttt gaacagtgct caactatcta ttggcgcaga cggagccatt aaatggagaa
      361 gaaattcaag ttatggatca gcttattcat ttgttatcac ttattcaatt taagggagga
      421 aatctaatga aagtagttta caaatcaatc aagccttacg gattcgagca aatcattttg
      481 aacaatcaag aaaatatccc tgaaaactgt acagagatta aaccaccagt tcctaactgg
      541 agaccaagat ttgattttga taaaaaacag tgggttga
//
LOCUS       DAFEOM010000212          578 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.221, whole genome shotgun sequence.
ACCESSION   DAFEOM010000212
VERSION     DAFEOM010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.221"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            <1..300
                     /locus_tag="LP524_002935"
     CDS             <1..300
                     /locus_tag="LP524_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL2235906.1"
                     /translation="FMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          206 a          101 c           69 g          202 t
ORIGIN      
        1 tttatgacta ggaaatggaa aaagcagtat ccaagagtcg tcaatttact cttgaatcct
       61 gccctattaa ccttttataa tttccctcac gccatcagac gaacaattta ttcgacgaac
      121 ctgattgaag gctttaataa gcagctaaaa cgatatactc gaagaaaaga acaattccct
      181 aatgaagaat ctctagagag attcttcatt tctcaattta atcaatataa ccaaaaattt
      241 ttaggtagaa ttcacaaggg atttaaagaa attcaggata cattagagtc gatgatttaa
      301 ctgtaattaa cagaatggat tttccattta catataattc ttgacgctac cataatctaa
      361 ataaccatca tattcatcta ctacttatat aattgagttt tattaaactt tttaagtttc
      421 tattctatca aatatttaaa caatctctgc atccgaaaag actacctcgt atagttttac
      481 taacaatatt tttattagcg tgttcaaatc ctgatttctt gtatcaatta ttttctgttc
      541 actttcaata agaaaaaagt ctttacttcc atggataa
//
LOCUS       DAFEOM010000213          529 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.223, whole genome shotgun sequence.
ACCESSION   DAFEOM010000213
VERSION     DAFEOM010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.223"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(5..90)
                     /locus_tag="LP524_002936"
     tRNA            complement(5..90)
                     /locus_tag="LP524_002936"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="LP524_002937"
     tRNA            complement(107..178)
                     /locus_tag="LP524_002937"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="LP524_002938"
     tRNA            complement(190..262)
                     /locus_tag="LP524_002938"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="LP524_002939"
     tRNA            complement(280..361)
                     /locus_tag="LP524_002939"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="LP524_002940"
     tRNA            complement(369..441)
                     /locus_tag="LP524_002940"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="LP524_002941"
     tRNA            complement(445..517)
                     /locus_tag="LP524_002941"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          136 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactta
//
LOCUS       DAFEOM010000214          511 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.denovo.224, whole genome shotgun sequence.
ACCESSION   DAFEOM010000214
VERSION     DAFEOM010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.denovo.224"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(<1..132)
                     /locus_tag="LP524_002942"
     CDS             complement(<1..132)
                     /locus_tag="LP524_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBL2235907.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          184 a           93 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag a
//
LOCUS       DAFEOM010000215        36263 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.guided.020, whole genome shotgun sequence.
ACCESSION   DAFEOM010000215
VERSION     DAFEOM010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.guided.020"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            <1..892
                     /locus_tag="LP524_002943"
     CDS             <1..892
                     /locus_tag="LP524_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBL2235908.1"
                     /translation="NKNDRGFISYDCKVTKEDLPNIIKNIIKEKIESGIHEKDICVLA
                     PQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFTESGEKVRRRKL
                     IANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTKHIFVNL
                     NIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTAHKVKGN
                     EYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSEVGYFTS
                     KGDLLYPTQLLDKIEFDYNI"
     gene            1092..1406
                     /locus_tag="LP524_002944"
     CDS             1092..1406
                     /locus_tag="LP524_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL2235909.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            1427..1804
                     /locus_tag="LP524_002945"
     CDS             1427..1804
                     /locus_tag="LP524_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL2235910.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1814..2587
                     /locus_tag="LP524_002946"
     CDS             1814..2587
                     /locus_tag="LP524_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235911.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2609..4012
                     /locus_tag="LP524_002947"
     CDS             2609..4012
                     /locus_tag="LP524_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBL2235912.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            4194..5378
                     /locus_tag="LP524_002948"
     CDS             4194..5378
                     /locus_tag="LP524_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBL2235913.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            5375..5665
                     /locus_tag="LP524_002949"
     CDS             5375..5665
                     /locus_tag="LP524_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235914.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5662..5883
                     /locus_tag="LP524_002950"
     CDS             5662..5883
                     /locus_tag="LP524_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235915.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5925..6704
                     /locus_tag="LP524_002951"
     CDS             5925..6704
                     /locus_tag="LP524_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HBL2235916.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6771..7265
                     /locus_tag="LP524_002952"
     CDS             6771..7265
                     /locus_tag="LP524_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBL2235917.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            7321..7959
                     /locus_tag="LP524_002953"
     CDS             7321..7959
                     /locus_tag="LP524_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235918.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            8031..8426
                     /locus_tag="LP524_002954"
     CDS             8031..8426
                     /locus_tag="LP524_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL2235919.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            8410..10863
                     /locus_tag="LP524_002955"
     CDS             8410..10863
                     /locus_tag="LP524_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL2235920.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10860..12887
                     /locus_tag="LP524_002956"
     CDS             10860..12887
                     /locus_tag="LP524_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL2235921.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12884..13906
                     /locus_tag="LP524_002957"
     CDS             12884..13906
                     /locus_tag="LP524_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBL2235922.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDGETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13923..14852
                     /locus_tag="LP524_002958"
     CDS             13923..14852
                     /locus_tag="LP524_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL2235923.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            15097..15213
                     /locus_tag="LP524_002959"
     CDS             15097..15213
                     /locus_tag="LP524_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBL2235924.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            15229..17148
                     /gene="tet(M)"
                     /locus_tag="LP524_002960"
     CDS             15229..17148
                     /gene="tet(M)"
                     /locus_tag="LP524_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBL2235925.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            17246..17434
                     /locus_tag="LP524_002961"
     CDS             17246..17434
                     /locus_tag="LP524_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBL2235926.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(17492..17845)
                     /locus_tag="LP524_002962"
     CDS             complement(17492..17845)
                     /locus_tag="LP524_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2235927.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            18376..18798
                     /locus_tag="LP524_002963"
     CDS             18376..18798
                     /locus_tag="LP524_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL2235928.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            18795..19025
                     /locus_tag="LP524_002964"
     CDS             18795..19025
                     /locus_tag="LP524_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2235929.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            19523..19723
                     /locus_tag="LP524_002965"
     CDS             19523..19723
                     /locus_tag="LP524_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBL2235930.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            19750..20943
                     /locus_tag="LP524_002966"
     CDS             19750..20943
                     /locus_tag="LP524_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL2235931.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(21011..22576)
                     /gene="guaA"
                     /locus_tag="LP524_002967"
     CDS             complement(21011..22576)
                     /gene="guaA"
                     /locus_tag="LP524_002967"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBL2235932.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(22635..23558)
                     /locus_tag="LP524_002968"
     CDS             complement(22635..23558)
                     /locus_tag="LP524_002968"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBL2235933.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            23754..24359
                     /locus_tag="LP524_002969"
     CDS             23754..24359
                     /locus_tag="LP524_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL2235934.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            24667..25683
                     /locus_tag="LP524_002970"
     CDS             24667..25683
                     /locus_tag="LP524_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBL2235935.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            25824..27125
                     /locus_tag="LP524_002971"
     CDS             25824..27125
                     /locus_tag="LP524_002971"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBL2235936.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            27171..27833
                     /gene="deoC"
                     /locus_tag="LP524_002972"
     CDS             27171..27833
                     /gene="deoC"
                     /locus_tag="LP524_002972"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBL2235937.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            27854..28252
                     /locus_tag="LP524_002973"
     CDS             27854..28252
                     /locus_tag="LP524_002973"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBL2235938.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            28318..29400
                     /locus_tag="LP524_002974"
     CDS             28318..29400
                     /locus_tag="LP524_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL2235939.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            29612..31174
                     /locus_tag="LP524_002975"
     CDS             29612..31174
                     /locus_tag="LP524_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL2235940.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            31167..32300
                     /locus_tag="LP524_002976"
     CDS             31167..32300
                     /locus_tag="LP524_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2235941.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            32300..33256
                     /locus_tag="LP524_002977"
     CDS             32300..33256
                     /locus_tag="LP524_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL2235942.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            33355..34521
                     /gene="deoB"
                     /locus_tag="LP524_002978"
     CDS             33355..34521
                     /gene="deoB"
                     /locus_tag="LP524_002978"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBL2235943.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            34545..35363
                     /locus_tag="LP524_002979"
     CDS             34545..35363
                     /locus_tag="LP524_002979"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBL2235944.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            35380..36087
                     /gene="deoD"
                     /locus_tag="LP524_002980"
     CDS             35380..36087
                     /gene="deoD"
                     /locus_tag="LP524_002980"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBL2235945.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT        11961 a         6273 c         7506 g        10523 t
ORIGIN      
        1 taataaaaac gaccgtggtt ttataagtta tgattgcaaa gttactaagg aggatttacc
       61 caacataata aaaaatataa taaaggaaaa gattgaaagc ggcattcatg aaaaagatat
      121 ttgtgtactt gctcctcaac actatcctct gttttcactg ggtgagtatt taaagaaaga
      181 attaccgtat tgcaattttg atgctataaa tatatcacca attaaggtaa ataatcatag
      241 tttatttttc aagttagcaa ttttgtactt tactgaatca ggtgaaaagg ttcgaagaag
      301 aaagctaatt gcaaacgata ttttaattat attaaaaaat gaatttggaa tagaagttaa
      361 agattatttt attgacttag atttattaca gctaataaac agtgtgaaaa gaatgtatag
      421 tggagacgac aacggtgttg atttatacaa atttgttacc aaacatatat ttgttaatct
      481 taatattgac gagaaaaatt acccgtctat ttttaagcac tatctatttt ttattaacga
      541 agtagaagat cggataaaaa ataattcatt atctagtagt gtatctagtt ttaagaagat
      601 gtttaaatct cgagatggta tcacacttac tacagcacat aaagttaaag gaaatgagta
      661 tgatactgtt atcgctatta atcttctaaa cgggaaaatt cctcattgga atgaaatttt
      721 taatgtttct gataaaggaa ttagttcagc gagaaaacta ctatacgtaa tttgttcaag
      781 agcaaaaaat aatttatttc ttttctcaga ggtgggttat tttacgagta aaggagactt
      841 gctttatccc actcaattat tagataaaat agaatttgat tataatattt aaaataccta
      901 ggtagtcgtt gttttaaata tagtaggaat cggacgctca gtacggagcg tctatttttt
      961 tacccaattt tgaaaggaag tgaacttatg aaaataaaaa atcaagaatc aaaaggtcgc
     1021 tctcccccct ttaaaaccat cacacaaata agattcattc aaacataaaa acagaaagga
     1081 taggtaaaaa tatggaactt aaatatgtca ttcccaatat ggaaaaaaca tttggacaat
     1141 tagaatatgc tggtgaaggg aacattgaac aacgtcgaat aaatggcaga ccaaccattc
     1201 tctctcgtag ttacaattta tactcaacga ttcaacgagc agatgatatt gttgtcattc
     1261 ttccctccga agctggcgag aaacattttg aatccgacga aaaagtcaaa ctcatcaacc
     1321 cacatattac cgcagaaggt tataaaattg gcaatcgagg atttaccaat tacattatgc
     1381 acgcagacga tatggtgaaa gcataagaaa ggaaaggtga aaaaatatga gattagcaga
     1441 aggtattgtg attgaaaaag accagacatt tggcacattg aaattttccg ccttgcgtcg
     1501 tgaggttcgt atccagaatg aagatggtac ggtttccgaa gaaattaaag aacgtaccta
     1561 tgacttaaaa tcaaaaggac aaggacgcat gatacaagtc agtatccccg cttctgttcc
     1621 tttgaaagaa tttgactaca acgcagaagt ggaattaatc aatcctgtgg cagataccgt
     1681 agcgacggct acttttcgag gggcagacgt tgattggtac gtgaaagcag acgatcttgt
     1741 attaaaagga cagacaaaac ctcatcagaa atataaaaaa gaagcgtccg aaagtgaaaa
     1801 ataggtgttg cttatgacaa acacaccatt gattttgaaa gaaattccga aggatgaagc
     1861 catttctttt attcggcaat accattattc aaagatactc cctagattgt gtaaatactt
     1921 tctagggatt ttttcagaag aaaaattatt aggcgttgtg gaattaggct ggggtacgca
     1981 acccctacaa accatacgca aactctttcc cgacagttcc cttcaaacca cagattattt
     2041 agaaattgga aagatgtgtt ttcttccaga aatgaatcaa accaattatt ttggcagtca
     2101 agctctttca gctctaatta agtggttgaa agaacatacg gactgtcatt ttttatatac
     2161 actggcagat ggaattgagg gaaaatgtgg ctatgtctat caagcgtcca acttctttta
     2221 ctgcgggtat ttcaagacaa gtgtctatcg tgataagcag tcatgggaaa agatacaccc
     2281 tcgaagtgct cgccttttat tagaggaaaa tgctcgcttt gaacaggtag agaaaaagca
     2341 ttggctttcc caggcatttt gcgaatacaa agggattgag aaaatcaatg gacgcatgtt
     2401 tcgctatctc tatccgttga caaaagaagc gaaaaaactg ttaggacaca ctctctatcg
     2461 acgtcattat tatccgaaag aaaaaaattt acgctttgaa aagcgaatcg cttatcagaa
     2521 atatgaagcc atttctcaac caacttttga taaacaggct cgtatctaca atacacaatt
     2581 attttgatag gtggaaagga ggatttttat gccattgctt catgacagag gagagcgaat
     2641 caaagccaac gataagcact tggtctatca ttttgtacta atttcattgc tggtagtttt
     2701 tctagtcgtc ctactccctt ttcattggag agcgttacaa tcggtgaact ggcaagcagt
     2761 tcagttagat acatttttct atcaacttca tacgccatac ttttggagca gtattctcac
     2821 tgctcttttt gtatgcggac ttatcagctg gctttattat cactatcgta tagattctat
     2881 aaaacagtta gagcaccgtc aaaagttagc tcgtatgatt ttagaaaatc attggtatga
     2941 agccgaagaa actcaatcag agggattctt taaagattta cccagcaaga aaaccaaaca
     3001 acgaatccgc cattttccta aaatatatta tcgaatgaaa gatggactaa tccatattca
     3061 tgtagaaatc accttaggga aatatcaaga tcagcttttg cacctagaaa acaaactaga
     3121 aacaggattg tattgtgaat tagtggaaaa aacgttgaaa gattcttatg tagagtatgt
     3181 cctactttat gatactattg gcaatcgaat tggcattgaa gacgtggtag ctcaagatgg
     3241 acgtgtcaaa ttgatggaat cggtctattg ggagttcgat tctctccctc acatgttaat
     3301 tgctgggggc actggtagtg gaaaatccta ttttattctc acattgattg aatctctcct
     3361 gcatacaaat gcaaagcttt atattcttga ccctaaaaat gctgatttgg cagacttagg
     3421 ggcggtcatg aataatgtct attaccgtaa agaagatatg ttggcatgta tcaatcaatt
     3481 ttacgaagat atgattgatc gcagtgaaac catgaaacaa caccctaact ataaaacagg
     3541 agaaaattat gcttatcttg gtttgtctgc caattttctt atttttgatg aatatgtggc
     3601 aatgatggat atgctaggtc gtgaaagtac caccgttatt cataaactca aacaaattgt
     3661 aatgttagga cgacaagcag gcttcttttt aattcttgcc tgtcaacgtc cagacgcaaa
     3721 atattttagt gatgggattc gggacaactt taacttccgt gtggctttgg gtcgtatgtc
     3781 tgaaatgggc tttggtatga tgttcggaag cgatacacaa aaacaatttt tcttaaaacc
     3841 catcaaggga agaggttacg tggatacagg aaaaagtgtg attagtgagt tttatacacc
     3901 ccttgtacca aaaaggtacg atttcttagg agaaatagga aaagtcattc aaaaaaagca
     3961 atctgagcct gtaccgcacg aagtgaaagg tacaggttca gacttgcttt gattggtgtg
     4021 gcgttagcca cgccaatcct ttagccccct ctatctaaca ggggggcaca aatacaactg
     4081 aaaacaggca attctcatca accaatgttg atggtacaaa ggattgcgga aaatatgaaa
     4141 ggtgtaaact caagggtcat agtttacatc ttttttagat tggaggtttg cgaatggaaa
     4201 acgaaaaaat ttggattaag gaattgaaag aaaagcgact ggcttatggc gtttctcaaa
     4261 ataaactggc ggttgcttct catattacca gaccttacct atcggatatt gaaacaggca
     4321 aggcagtccc tactcaacag gtgaaagaag atttactgaa cgctttggaa cgcttcaatc
     4381 ccgataaccc attggagatg ttattcgact atgtacggat tcgattccct accaatgatg
     4441 tggctcaaat tatcggtgag gttttacgtt tgaatatgga ttatatgcta cacgaagact
     4501 ttggctatta ttcttatccc gaacactatc gtttcggtga tattgttgtc ctagtctctc
     4561 atgatgtaag taaaggtgtt ttattagaac tgaaaggaaa aggctgtcgg caatttgaaa
     4621 actttctatt ggctcaacat cggagctggt atgatttctt tcaagattgc atggaacata
     4681 aaggtatttt caaacgcttg gatttagcta tcaacgataa aacagggatt ttgaatattc
     4741 cagatttggc aaagaaatgt aaacaggaag aatgcatttc cgtctttcga agctttaaaa
     4801 attatcgcag tggtgaattg gttcaccgtg atgaaaaacc atacatggga aacacacttt
     4861 atattggctc attaaaaagt gaagtctatt tctgtatata tgagaaagat tatgagcaat
     4921 acgtgaaaaa tgatgttccc ttagaagacg cagaagtaaa gaatcggttt gaaattcggt
     4981 taaaaaatga ccgagccacc caagccatga aagatttatt ggcacatcaa caggcggaaa
     5041 aaacagcctt tgaaattatc aatcgctata tccgctttgc agataaagac gacactaaac
     5101 gtcggagtga ttggaaaact aatgaacgtt gggaatggtt tatcggtaaa aatcgtggag
     5161 cattacgcct aaccacacag ccagagcctt attcttttga acgaacattg aactggttac
     5221 atcatcaagt cgcccctaca ttaaaaatcg caagcatttt agatgttctc aacggtacaa
     5281 ccattatctc caccatgatt caggaagcca aactgacaga aaaacatgag aaattaattg
     5341 aacaacaaca tctagccatg gaggatttga ttacatgagc cttttaacaa aagaattaaa
     5401 gaagttaggg tttcagtgtg gcattgagtt tcaagcgtat atacaaaata cagggaaata
     5461 tacgtctctc attattgagg gaaaacggca agctggtgat accatctata cctatgattt
     5521 ttacaaggtg agattttata ataattatac caatcgagta accgtctatg gagagcattt
     5581 aacccctttt caattattaa ggcgtgtaaa aagctatatc tattatcgag aaaaatatct
     5641 taaggaaagg aggacaatca catgaatttt gggcagaatt tgtacaactg gttcttggat
     5701 aatgcacagt cattggtatt gttagcgatt gttgtcattg gactgttttt aggttttaaa
     5761 cgagaatttt caaaactcat tggcttcttg attattgctc taatcgccgt cggacttgtg
     5821 ttcaatgcag ctggcgtgaa agatgtgtta ttaaacttat ttaaccgaat cattggtgct
     5881 taacagatat ttttcatgat aaaataggaa gtatatattt tgttatgaaa ggatttatga
     5941 atatgtttac aaatgatcaa tttaaatctc ttttaattaa aacattagaa agaaagagct
     6001 cttcaaatta ttatgaagga ggtaacgaga ttgtttctaa aatgaaaata tcagaggtaa
     6061 ctttagatga gactgacgat tttacttatt ataaaggata taaaaagggc tggaatacgc
     6121 tttgcacata tcttaaaata agagttcctt ttgatgattt agactttttt gaaagtcata
     6181 aagatttgat tacccagaca gcaagctcca tatatgataa acaaggtgat aatgtattag
     6241 tggatactat tttagtacca cttccagaga attacgaagt aattaacttt agtcagttga
     6301 aaattagtga tgtggtttca caagctattg aagacgctga gtcttttatg agtaatggtg
     6361 aatatcaacg tgcctttgac agagttcaca ctgcttttca tggatattta attgaaatac
     6421 taaaaaaata tgaaattaca gttcctagag acgaaaatct atctaaatta tatagtagga
     6481 tacaacaatt aattgaaaaa gaaattcaac ctactgaact tgctgatata gtgaagacaa
     6541 cgataaggtc atcaaatgga atgattagct ctcttaatga agcaagaaat aggcattcac
     6601 ttgcacaccc taatacaaat attattggaa agagagaagc aaaactaata ataggtattt
     6661 caagtacagt tacagattat attagcggat accttgataa gtagcgtcat taactgattg
     6721 ctactttttt tatgactatt tttataaaaa ggatgtgata agaattggaa atgaaagtct
     6781 atgtggcaaa tttaggacgg tataatgaag gtgaattagt cggagcatgg tttactccac
     6841 caattgatga ggaagaaatg gcagaaagaa tcggtttaaa tgaggactac gaagaatatg
     6901 caatacatga ttttgagctt ccttttgatg tggacgaata cacgccgatt agtgaaatca
     6961 accgtctatg tgaagccatt caagaaatcg aaggtacacc tatttataat gaactcaaag
     7021 aaattcaagg catgtggttt agtagcttgg aagaattact ggaaaacaaa gaggatattc
     7081 attgttatag tgattgtgat tcgatggaag atgtcgcccg ttattacgtg gaagaaacag
     7141 gacagcttgg tgaagtccct tccaatttac aaaattatat tgactaccaa tctttgggac
     7201 gtgatatgga aatagagggt aattacctag ttacctctca tggggtattt gaatattgtc
     7261 agtaagttta acaatccgtg ttataattaa attaataaat agcacggaga ggtataaatt
     7321 atggtaaatg tatttgaaaa tcttaaacta ttaaaactag acatgaaaga taaaggctgg
     7381 gtgatagatt ccttttactt tcgttacaaa cagcaaaatt atattgtact tgttaagcta
     7441 tttgaaaaag aggaaaaagt tccagaatat gctctgttaa aactagaatt tttaaaggaa
     7501 aacgattttt cagatatgct agctgtctat gctaattcgg taaaattata tacagacacg
     7561 aaaacaataa gagaatattt tggtatcgaa tacagtagta acttgggaga tgttttattt
     7621 caatttagcc aaactcttgc tcgatttatt ccgacagaag ttagtgagaa gaaaaatgaa
     7681 gaccaaaaag aagccatgtg tttctcatta agtcaaagtg attcagaaga tcccagaaaa
     7741 aaatattgtt tttcagttag aagaaatcct ttaaagggaa atggtgagct gggaaaacga
     7801 agtcctttta atgacaataa aacgagattg tatagaccca gcttatatga acgattaggg
     7861 tcagatacca atttaagttt cagatattca atgaatccag atgatgaaga aactgatgaa
     7921 ggtattattg caaaatggac aaaaaataaa attgaatagt taaagcattg aaagagcaat
     7981 cacctatttt gattgttctt tttctatttt tacggaaagg acgtgaagac atgaaaaaaa
     8041 taagaagtta caccagtatt tggtcagtag aaaaagtgat ttatgcgatt aatgatttac
     8101 aactcccttt cccaattacc tttacgcaaa tgacatggtt tatcgtttct ttgtttgtcg
     8161 tcatactctt tggcagttta ccaccacttt ctctgattga cggagcacta ctcaaatact
     8221 tagggattcc cattgttctc actgggttca tgtgccaaaa gacctttgac ggaaagaaac
     8281 cctatggatt tctaaagtct atcattacct atgctctacg ccctaaagta acctacgctg
     8341 gcaaacaaat acgattaaaa aaagaaacaa tgaatgaatc gattacgatt gtaaggagtg
     8401 aacaactaga tggcatatcc gattaaatat atggaagata atctcgtatt taatcatgat
     8461 ggcgaagtat tcgcttatta tgaattaatt ccctataatt acagtttttt aagttccgat
     8521 gaaaaaattc aagttcatga taatttctgt caattgattg cccaaaatcg tgatggcaag
     8581 attcatgctc tgcaactagc aaccgaatca agtattcgag ccacacagga acaatcgaaa
     8641 aaagaaatca ctggacgatt aaaagagata gcttacgaaa aaatagatga acagaccgaa
     8701 gcgttaattt ctatgattgg tgacaatcaa gtagactatc gcttttttat tggttttaaa
     8761 ctctacctca atgaacagga agtatccgta aaaaatgttg ggaaagaaat tcaaaaagca
     8821 tttgcggaat tctttcatgg tgtgaatcac aaattaatgg gcgattttgt ttctatgcct
     8881 atggcggaaa tcgaacgctt ttctaagatg gaacagttgt tagaaagtaa attatccaga
     8941 cgctttcaga ttcgtaaatt ggagaaagat gattttggtt atattattga acatctttac
     9001 gggcaaacag gtgtcccttt tgatgactac gactaccatt taccgaaacg atacgaagat
     9061 aaaaaagtgt tggtaaagaa atacgatatt ctcaaaccca ctcgctgttt aatcgaagaa
     9121 aatcaacgct atttacggat tgaacaggaa gataccacta cttatgtggc gtattttaca
     9181 attaatagta ttgttggtga attggatttc cctaatagtg aaatttttta ttaccagcaa
     9241 cagcagttta ctttccccgt tgataccagt atgaatgttg aaattgtcgc caataaaaaa
     9301 gccttatcta ccgttcgcaa taaaaagaaa gaactgaaag atttagatag ccacgcatgg
     9361 gaaagcgaca acgaaaccag tactaatgtg gtggacgctt tagaatctgt cgatgaatta
     9421 gaaagtaatt tagaccaaac caaagaatct atgtacaaac taagctatgt ggtacgggta
     9481 tcagcaaaga atttggacga gctgaaacaa cgttgtgatg aagtaaaaga tttttatgat
     9541 gatctgtcta tcaaattagt acgacctttt ggcgatatgc taggcttgca taatgaattt
     9601 attccctcaa gcaagcgata tatcaatgac tatattcaat atgtcacgtc ggattttttg
     9661 gctggtcttg gtttcggtgc tacacaaatg attggtgaaa attctggtat ttacgtcggt
     9721 tacaatctcg atacaggcag aaatatttat ctccaaccta gccttgcaag tcaaggaatt
     9781 aaaggctctg taacaaatgc attagcgtcc gcttttattg gttcattagg tggcggaaaa
     9841 tcattctcta acaatatgct ggtctattat tcagtactct ttggcggaca ggcaaccatt
     9901 gttgacccaa aagctgaacg tggtgaatgg aaagaaacct tacctgatat tgcccatgaa
     9961 atcaatattg ttaatctcac gagtgagaac gacaataaag ggttacttga cccttatgtg
    10021 attttaaaac tcccgaaaga ttcagaaagc cttgccattg atattctcac gttcttaaca
    10081 ggtatttcca gccgagatag cgagaagttt cctgtcctgc gtaaagctat tcggagtgta
    10141 actcaaagca aacaacgagg gttgttattg gtgattgatg aattgcgaaa agaagatacg
    10201 cccatcagca atagtattgc tgaccatatc gaaagctttg tcgattatga ttttgcccat
    10261 ctgttattca gtgatggcag tgtcacccaa tccattagct tggaaaaaca actcaatatt
    10321 attcaagtgg cagatttggt cttacccgat tccaatagct cttttgaaga atacactacc
    10381 atggaattat taagtgtggc aatgctgatt gtcattagta cctttgcttt agactttatc
    10441 cactctgacc gttccatttt taaaattgtt gaccttgatg aggcttggag ttttttacaa
    10501 gtggcacaag gaaaagctct atctatgcga ttggttcgag caggacgtgc catgaatgca
    10561 ggggtctatt tcgttactca aaacgcggac gatttactag atgaaaagat gaagaataat
    10621 attggcttga aatttgcctt tcgttccact gacctagtgg aaatcaaaaa gaccttagaa
    10681 ttttttggtg tggacaagga agatgaaaac aatcagaaac gcttacgtga attagaaaat
    10741 ggacaatgtt tagtttcaga catctatggt cgtgtagggg ttatgcagtt tcacccgatt
    10801 tttgaagagt tgcttcatgc ctttgatacc agaccgcctg tgcgaaatga ggtggaagaa
    10861 tgacacaaaa acaaaagaaa tttcttcact atagttggat aacgctcctt tgtgtgggcg
    10921 ttattttact tttattaggc acgctgggca atgcagtaca agcgacaggc ttagttgatg
    10981 aaaccattga tacaagcaat gaatactcca agtatggttt gaatcaatat cagcttgatt
    11041 attatgtgga caatagctgg ggctggcttc catggaattg gagtgatggc ataggacaat
    11101 cggtgatgta tggattgtat gccattacta attttatttg gacaatttct ctttatctat
    11161 cgaacgccac aggttatttg gtgaaagaag cctatgcctt agatttcatt agtcaaacat
    11221 cccaagcaat tggcgaaaac atgcagacga ttgcaggcat tacaccgaat ggcttttcca
    11281 gttcagggtt ttatgtcggt ttcctccttc tttttattct cattgttgga atctatgttg
    11341 cctatacagg attggttaag cgagaaacga cgaaagtgat acgagctgtt ttaaactttg
    11401 tcgtggtgtt tattctatcg gcttccttta ttgcgtatgc acccgattat attgcgaaaa
    11461 tcaatgattt ttccaaagat gtcagtaccg ccagtctgaa tattgggaca aaaattgttc
    11521 ttcctaacac ggatagtcaa gggaaagaca gtgtggacat gatacgtgac agcttattct
    11581 ctgttcaagt aaagcaacca tggctattgc ttcaatatgg ctccactgat gtagaagcgt
    11641 taggggaaga acgtgtggaa agtttattgt ctaccagtcc agatacaaac aatggtgaag
    11701 atagagaaaa tgtagtgatt gaagaaattg aagatcatga caatacctat ttaaccctac
    11761 cgaagacgat ttccagatta ggaacagtct tctttttgtt tgtattcaat attggtatct
    11821 cgatttttgt tttccttcta acaggaatca tgattttttc gcaagtgcta tttattattt
    11881 tcgctatgtt tttgccaatc agtttcctat taagtatgtt gccgagtttt gatggcatgg
    11941 gcaggcatgc cattaccaag ttatttaatg tgattatgac aagggctggt attaccttga
    12001 ttattaccgt tgcctttagt atttccacta tgctttattc gctgtcagca agttctccct
    12061 ttttcatgat tatgttttta caaattgtga cctttgcagg gatttatttt aaactgggcg
    12121 atttaatgag cctattcact ttacaaagca acgactctca aaacatgggc agacaagtgt
    12181 ttcgcagacc tcgtcaaatg atgaatcgac aaaccagaag atttcaacgc agtatgagta
    12241 agatttttcg tggaaaagca gggtcaacga agcaaaagac aatgacaaca aagcagaatc
    12301 aaaccaataa agctaaccat actcgtcaaa atgaacggac tacaccaaag aaagaaacag
    12361 cactttccaa tgtgaaaaag caacaaggcg gtatcgggca acgattaggt gaaaagacag
    12421 cccatgtatt ggacacgaaa aatcgtctag tcaataaaag aaagcaggca aaacaacaga
    12481 tacaggcgac accaacccat gtcaaatatg cccttcatca aggaaaagag aatgtcactc
    12541 ataatgtaac agactttaaa caatctatgg cagataccaa agctcaacgc aaacaacagc
    12601 gtatgaacca gcaggagcaa aaacgaaaga acattgctca aaagcgtttg gagctggata
    12661 aagcaaaaga aaaaagacga aaagaaaacc catcatctcc tgttcagaat aaccagcagc
    12721 atgttcgcaa gcgtcagcaa accgtcacgc ctgttttgtc acaaaacact aaacgtgaaa
    12781 atcatcagcg accattgaaa aaagagcaaa aacaaaagac accaccgaag caattcaatc
    12841 agaggaagct aaataaaaga acggataagc gaggacaaca aacatgaagc taaaaataac
    12901 agtgatttgt agtgtggtct tctctctctt cttttttctc ttattattcc tagtcatttt
    12961 ctttgcagat gatacggaca gtggcgaaaa caacaaggat tcttctattc cacaaggcgg
    13021 tgtgaccgtt tcaccagaag tgttggcaca ccgtccgttg attgaaaaat atggtaagga
    13081 atatgggatt gaagactatg tttcttatat cttagccatt atgcaagtgg aatcgggcgg
    13141 tacagcagaa gatgttatgc aaagttccga atctttgggt ttaccgccca acagtttgag
    13201 taccgaagag tccatcaaac aaggggttaa atatttcagt gaattattaa ccagtgcaga
    13261 acaacaagga gtggatatag attctgttat ccaatcttat aattatggcg gtggcttttt
    13321 aaattatgtg aggagtcatg ggaaaaaata tacctatgaa ttagccgaac agttttctaa
    13381 agagaagtcg ggtggtcaaa aagcagacta tcccaatcct atagccatac ctgtgaatgg
    13441 tggttggcga tataactatg gaaatcagtt ttatgttcag ttggtttcac aatacctaac
    13501 cgacacttcc ccaacagaat ttgatggtga aaccgtgcaa gtcattatgg acgaagcctt
    13561 gaaatacgaa gggtttccct atgtctttgg aggggcgtcg cctaccacct cttttgattg
    13621 tagtggctta atacaatggg tgtatgacaa agctggcatt tctctccctc gtgtcgccca
    13681 agatcagtat gacgctacgc aagaaatctc tatggaagaa gcccaagcag gcgaccttat
    13741 cttctttcat tcaacctata atgcaggcac atatgtgact catgtggcga tatatttaga
    13801 gggaaatcgc ttttatcatg caggcgaccc aatcggttat ggcgacttaa gcagtcgtta
    13861 ttggcaagac catctgattg gggctagacg tgttattcat aactaagaaa ggaatgaatc
    13921 aaatgattat aaaaatcgag cgaaaagaaa agaaggagaa accaaagaaa tctaaaaaac
    13981 cgagcaagca aagaaaagtt ccgatgatta aagttggcac gcataagaaa ttaacctttg
    14041 ttttatgggt tttattgatt ggtagtgtcg gatttggaat ctataaaaat tttactgcca
    14101 ttgacactca tactgtccgt gaaacagaaa tcattaaaca gcagattgtc gataccaacc
    14161 aagtagaaag ttttgtgaaa tcctttgcga aagactactt ttcgtggcaa caatcccaag
    14221 aagccattga taaacgaaat gaaaaattga cccattattt gacggaagaa ttgcaggtat
    14281 taaatgaaga aatgattcgt aaggatattc ctactagttc tagtgtgaac gatatacagg
    14341 tgtggcaggt ttctcaagtg aatgagaaca cctttgaggt actgttttct gttgaacaag
    14401 ttattaccga agacaaagac aaggaaacta tatcgtctag ctttcatgtg gttgtccata
    14461 tagatgaatc agataatatg gttattatca aaaatccgac aatgagtaag aaacctcaaa
    14521 aatcggacta ccaacccaaa cagctagaaa gtgaccatac agtagataca gaaacgatgg
    14581 acgaaatcac tagcttttta gaaacgttct tccagctcta tcctaccgcc acagaaaaag
    14641 aattgactta ctatgtaagt aatcatgtgt tacctatgat taacaaggaa tatgttttcg
    14701 aggaattggt gaatcctatc tttaccagaa aagataacca agtaatcgtg aatgtcgctg
    14761 tgaaatattt agatcaagaa acaaaggcaa cccaaatctc gcaatttgag ttgatattag
    14821 aaaaacaaga taattggaag attgtaaaat aacaaatatt ggtacatgat tacagatact
    14881 ttgtaatcat gtactctttt tgataaaaaa ttggagattc ctttacaaat atgctcttac
    14941 gtgctattat ttaagtgact atttaaaagg agttaataaa tatgcggcaa ggtattctta
    15001 aataaactgt caatttgata gcgggaacaa ataattagat gtcctttttt aggagggctt
    15061 agttttttgt acccagttta agaatacctt tatcatgtga ttctaaagta tccagagaat
    15121 atctgtatgc tttgtatacc tatggttatg cataaaaatc ccagtgataa aagtatttat
    15181 cactgggatt tttatgccct tttgggtttt tgaatggagg aaaatcacat gaaaattatt
    15241 aatattggag ttttagctca tgttgatgca ggaaaaacta ccttaacaga aagcttatta
    15301 tataacagtg gagcgattac agaattagga agcgtggaca aaggtacaac gaggacggat
    15361 aatacgcttt tagaacgtca gagaggaatt acaattcaga caggaataac ctcttttcag
    15421 tgggaaaata cgaaggtgaa catcatagac acgccaggac atatggattt cttagcagaa
    15481 gtatatcgtt cattatcagt tttagatggg gcaattctac tgatttctgc aaaagatggc
    15541 gtacaagcac aaactcgtat attatttcat gcacttagga aaatggggat tcccacaatc
    15601 ttttttatca ataagattga ccaaaatgga attgatttat caacggttta tcaggatatt
    15661 aaagagaaac tttctgccga aattgtaatc aaacagaagg tagaactgta tcctaatatg
    15721 tgtgtgacga actttaccga atctgaacaa tgggatacgg taatagaggg aaacgatgac
    15781 cttttagaga aatatatgtc cggtaaatca ttagaagcat tggaactcga acaagaggaa
    15841 agcataagat ttcagaattg ttctctgttc cctctttatc atggaagtgc aaaaagtaat
    15901 atagggattg ataaccttat agaagttatt actaataaat tttattcatc aacacatcga
    15961 ggtccgtctg aactttgcgg aaatgttttc aaaattgaat atacaaaaaa aagacaacgt
    16021 cttgcatata tacgccttta tagtggagta ctacatttac gagattcggt tagagtatca
    16081 gaaaaagaaa aaataaaagt tacagaaatg tatacttcaa taaatggtga attatgtaag
    16141 attgatagag cttattctgg agaaattgtt attttgcaaa atgagttttt gaagttaaat
    16201 agtgttcttg gagatacaaa actattgcca cagagaaaaa agattgaaaa tccgcaccct
    16261 ctactacaaa caactgttga accgagtaaa cctgaacaga gagaaatgtt gcttgatgcc
    16321 cttttggaaa tctcagatag tgatccgctt ctacgatatt acgtggattc tacgacacat
    16381 gaaattatac tttctttctt agggaaagta caaatggaag tgattagtgc actgttgcaa
    16441 gaaaagtatc atgtggagat agaactaaaa gagcctacag tcatttatat ggagagaccg
    16501 ttaaaaaatg cagaatatac cattcacatc gaagtgccgc caaatccttt ctgggcttcc
    16561 attggtttat ctgtatcacc gcttccgttg ggaagtggaa tgcagtatga gagctcggtt
    16621 tctcttggat acttaaatca atcatttcaa aatgcagtta tggaagggat acgctatggt
    16681 tgcgaacaag gattatatgg ttggaatgtg acggattgta aaatctgttt taagtacggt
    16741 ttatactata gccctgttag tactccagca gattttcgga tgcttactcc tattgtactg
    16801 gagcaagcct ttagaaaagc tggaacagaa ttgttagagc catatcttag ttttaaagtt
    16861 tatgcaccac aggaatatct ttcacgggca tataacgatg ctcccaaata ttgtgcaaat
    16921 atcgtaaata ctcaactgaa aaataatgag gtcattatta ttggagaaat tcctgctcga
    16981 tgtattcaag attatcgcaa tgatttaact ttttttacaa atgggcttag tgtttgttta
    17041 gcagagctaa aaggatatca ggttaccact ggcgaacctg tttgccagac ccgtcgtcta
    17101 aatagtcgga tagataaagt aagatatatg ttcaataaaa taacttagtg cgttttatgt
    17161 tgttatataa atatggtttc ttattaaata agatgaaata ttctttaata tagatttgaa
    17221 ttaaagtgga aaggaggaga ttgttattat aaactacaag tggatattgt gtcctatttg
    17281 tggaaataaa acaagactac gaatacgagt ggatactata cttaaaaatt tccctttata
    17341 ctgccccaaa tgtaagaacg aaactttaat taatgttcaa aaaatgaata taataacaat
    17401 caaagagcca gacgccaaga cgcagagccg ataatttgag aaatgaaact ctcatcttat
    17461 cggctctttt tgtttatctg aattttactg actagccttc aatatttctt gcttcattga
    17521 taccttttgc tagtgcttcc attaaggata gttctttgtc tgtaaagcta tccatgtatt
    17581 tttctacctg taaccttcga gtgcttttaa tcaaattatc tgtaggtaag aaaaattcat
    17641 caatagatac atggagtaat gatacaagat catacagcac ttgtgtactt gggtgttgtc
    17701 ctttgttttc tatatttgtt aaataacgtg ggtcaatttc aatcattgtt cccacttgtt
    17761 cacgagtgag accccgtttc attcgagcct ctttaatcgc taaaccaacg gctctaaaat
    17821 cgtatttatc ttctttttta cgcatactaa atcacctcta tacattttac tgttcctata
    17881 taaccataaa caggtataga aaaacatgtt atatagttta tgagttccta tagaacactg
    17941 tggaatttat taatgcgatg aatccaattt caaatagaaa ggtcgctgat tttatttacc
    18001 tatacaactt tataaaattt agtcatactc ctagaaagta gataaaattg gcgacttaat
    18061 ggagggaaaa gtcgccatga gaaagataat ctgtcatcta gttctcttaa ttaagttctt
    18121 atcaaaaaaa cttatccacc tgtactggat agatatgacc atcaatgtgg ttttgcagat
    18181 tacatagata gaaaaatcaa agcatatgaa cagagaaaac aatctgttta tgtgcttttt
    18241 tggtcactta ataaatatct tttaggattt ctcttcattt atgcaacaaa tccccctttc
    18301 gtgtcggggt ttagtatgaa gggagataaa gaagcacatg ccactccctt tcacgacaga
    18361 aagggggtga aaagcatgaa accatcttct tttcaagaaa ccatagaaaa tcagtttgac
    18421 tatatctgca aacaagttat tgaagatgaa cgaaaagatt acttcaaaca tctagacaga
    18481 ctatcagcaa aagaagtctc gttttctgat atagataact accttgtcaa ctctttttct
    18541 actgttgata cgtacgtcac tgatttccaa ctatttaaat tatttggatt aagaatcaat
    18601 gttgaaagtg atttattaag tgaagccctg cgtcatcttt cagagaaaaa acggaacatt
    18661 attttacttc attactttat ggatatgagt gatgtggaga ttgcggagtt actaaatttg
    18721 aatcgttcga ctgtttatcg tcatcgaata agtggactag ccatgattaa agaattcatg
    18781 aaggagtgta aagaatgaaa acagcttatc caagggttcc cttccccctc attgtaaagg
    18841 cgacagatgg cgacgtggaa gcaattaatc agattgttaa acattatcga gggtatacct
    18901 caaaacgttc attacgtcgc atgacagacg aatatggaaa ttctcatatg gttattgatg
    18961 aaaccctacg tggcagaatg gaaacgaaac tcatctctaa gattttatcg ttcgaaatta
    19021 aatagtccca ttttttcgtg gaagcgtgtc gaatcgtatc cacgcttccc gatttgggag
    19081 gtttgttatt ccatcaaagc atattgtgtg ttcagtaggt tttgataggc taacaagcca
    19141 aacgttcttt gacaaccaaa taaaggcaat cgaatacgtt tcggtaagaa aagagctgat
    19201 gaactaacac gccatgacct acaaaattag tgagcgattc atgttagtaa ttcgaaagta
    19261 atctttagca ggattgctgc catgactttc ttatcgtgat aatgatactc ccgtatagcc
    19321 atagtccgag cgttagaagc gtcacaggca atgggtacgg ctacatgaga accatgcaga
    19381 agtgaaattc ctgtgagctt tgctagagct gttcgattgc taagatattc ttctcttgtc
    19441 agtgtattat tataaaacta tttgatataa ttataggtga atattggagt tttcctagcc
    19501 tataattggg aaaggagtaa taatgataac ggtcacaaaa aaggatttag ttgagctagg
    19561 atatggaact tctttttcag cagacatcat aaaaaaagca aaagaattga tgattacaaa
    19621 agggcatatc tactatcaat ctcgaaagct agatagagtc cctcgtgaag ctgttgaaga
    19681 aattctagga attcggtttt caaatgaaaa caggtctgag tagtttttgc acagtaagga
    19741 gtaaaaacta tggttaaaga cccaataaaa aaagcaaaga atggaaccta ttattttcgt
    19801 gcaaatctag gttatgattc taaagggaaa aaaattcaaa aatatcgtag tggattcact
    19861 actaaaaaag aagcaagaga agaatattcg aaactgctat tgatgaaacc agaagaatta
    19921 agtgagaata aggataaaat gacgtttaaa cattatatct ttgaaatttt ccttccatgg
    19981 tataagacac aggtaaagct cagaacatat gaaaatcgct taccaactat taagaagcat
    20041 ttctcttatt ttgataaaat ggctgtttcc gatattgagc ctattgatgt gcaaaattgg
    20101 caactaaaat tatcaaagaa gtgtaagtct tcttatgtaa gagctgttca aggactattt
    20161 tcagttgcta tggatcgagc aattgtcctt gggataacca ccacaaatcc atctaagatt
    20221 attggcaatg tgaaaaagca aaagtctaaa attgaatttt ggacaaaaga agaatttgaa
    20281 aaagtaatct ctctaattta caaagaagat tactatcaac actttttatt tatctctctg
    20341 tggtttttat ttatgactgg aatgcgaatt ggtgaagcta ctgccattca atgggaagat
    20401 attgattttg actcaggagt actaaccatc aacaaaacac tctattacaa gaatcaatcg
    20461 aattatagat ttgttgaacc aaaaacaaag gcgagcgttc gtcatattgt attagataaa
    20521 tgcacattaa cttatttatc cgaatggaag gaagtacaac aaaatctaat tcagacagac
    20581 tttgtaatga gctataacgg tattcctacc caaaaacata cactatcata tgctattgaa
    20641 cgctatgcta agctggctgg tatccatcga atcagaatcc atgggttacg gcattctcat
    20701 gcttccttgt taatcagtat gggagaaaat ccacttatca ttaaagatcg tctaggacac
    20761 gaagatattg agacgacttt aggaacgtat ggacatcttt atcctaacag taatttcgag
    20821 gtagctaaca aattaaatgg agccatttcc tttaaagaag caaaacaaaa ttatgatact
    20881 tcaccaaaga atcaatttac tgtagactac ttgcgaaaca aagaaatgaa aaaagtgcaa
    20941 taaaagtgca gtaaaaagat aagaagagcc agaaaccctt ggtataagag gatttctagc
    21001 tctcctacaa ctattcccac tccactgttg ctggtggttt agaagtgatg tcatacacga
    21061 tacggttgac gtgggctact tcgtttacga tacgtacaga gattttttgc aagacttccc
    21121 atgggatacg ggcaaagtca gcggtcattc cgtcgattga agttacagca cggataccga
    21181 ctgtgtagtc gtaagtacgt ccgtcaccca taacaccgac agagcggatc cctggaagaa
    21241 cagtgaaata ttgccagata tcacggtcaa gtccagcagc tgcgatttct tcacgcaaga
    21301 tagcatcaga ttcgcgtacg atttctagct tgtcttcagt gatttcgcca agaacacgaa
    21361 taccaagacc aggaccaggg aatggctgac gccaaacgat tgaatcaggc atgcctaact
    21421 gagtccctaa ttcacggact tcgtctttga ataatgtgtt taatggttcg atcaattcaa
    21481 attgcatatc ttctggcagt ccacctacat tatggtgaga tttgatcgtc tgtgctgttt
    21541 ctgtaccaga ttcgataacg tcagtataca acgttccttg tgctaggaaa gagacgcctt
    21601 cttcaccagc aagttttgtt gcttcgtcat caaaaacata tacgaattca ttcccaatga
    21661 ttttacgttt ttgttctggg tcagatacac ctttcaactt gtctaagaag cgatctcttg
    21721 catcgacttt gatgatgttc aagccgaatt ttccgcctag actttccatt acttgttccg
    21781 cttcgccttt gcgtagcaga ccgtggtcaa caaagataga tgtcaattgg tcaccgatcg
    21841 ctttttgtaa aagaacccca acgacactag aatcaacgcc acctgaaaga ccaagcaaga
    21901 cttttttgtc accaacttgt tcacggattt tttctacttg catatcgata aagttatcca
    21961 ttgtccagtt accttcacag tgacaaacat caaaggcaaa gtggcgcagc aattcgattc
    22021 cgtattcgga atgacgtact tctgcatgga attggatacc ataaaaatta cgtgttgtgt
    22081 cttggataga agcaatcgga caatctttgc ttgttccgac tgtttcaaag ccttcaggaa
    22141 ctttagttac caagtcacca tggctcatcc atactgtttg ttgttttggt gtttctttaa
    22201 acaagacagc ttgatcgctt gttacttcta acaaggcttt cccatattcg cggtttttag
    22261 caggctctac ggaaccgcca agtttgtacg tgatcaattg cataccatag caaatcccta
    22321 atacaggaat acctaaattg aagatctcag gatcgatccc aaatgcattc tcatcatata
    22381 cactgtttgg tccgccagaa agaatgattc cctttggatt catcgctttt acttcttctg
    22441 cagtaatgcg gtggctcaat aattcggaaa agactccaag atcacgaata cgtcttgtga
    22501 tcaattggtt atactgacta ccgtagtcta atacgataat cttttcaacg ttcgtcatat
    22561 cggcaacgtt tgtcaaattt attaccccta tccctttaaa ttttatctca aaatagtcaa
    22621 actattttaa aatgctaata ttttcgcatg aaaatcgaat cgatgatatg attttcagtt
    22681 ttatggagaa ttatatcggc tcttcctcgt gtaggcaaga tatattcctg taaatttttc
    22741 aaattcacat ttttccatac atcttttgcc attttaaagg cttcttcacg atctccgata
    22801 gcaaaacgat aataataatt ctctggattt tggaaagccg tatccagtaa cgcaccaaaa
    22861 cgatccaaat accatttttc gatcagcacg ggatcggcat cgacatagac agaaaaatca
    22921 aaaaagtcgc tgacgtagat ttgctgattc gcaggtaatt gaagcgtatt gatcccctca
    22981 acgatcaaga tatcaggctg ctggatcaat tcgtacttgt ctggaatgat atcatacaca
    23041 tcatgtgagt aaacaggtac ttttatctct tcctgaccag attttacacg gttcaagaaa
    23101 tcgatcagca tctccatatc ataactttca ggaaatcctt tacggtccat gattccacgc
    23161 tcttttaata tacgatttgg ataaagaaat ccgtcagtcg tgatcagctg aacgtttctt
    23221 cgtttgaagg tacgagacaa aatcgtctgc agcagtctgg ctgttgtact ttttccaacg
    23281 gctacacttc cggcgatccc gataataaaa ggcggtactt ctacgtattt atgaagaaat
    23341 aaacctttac tcaagaacaa agattcaaat tctttcatat aaagataaat cagatgagca
    23401 agaggtacat agatatctcg aacatcctgc atggaaatgc ggtcattcaa acttttgata
    23461 ttatctagtt catcttgtgt caaaggtgct tgcccattac gataaaattc tcgccattct
    23521 tctcttgcca cacggtagta gttcattgga tcattcatat tagcctcctg cgttctccca
    23581 tcaatggaac tattttaaca caaaataaac gcaaacccca ccacttaaga gagattgccg
    23641 atctttttgt ataatcttta taatagaaag aaacggagtc ttattcttga cttgcgcacg
    23701 gatacataaa aggatagact gttgtcatga aagaaatgag aaggatggaa gacatgacaa
    23761 atcattacta ttctgaacgc ccagatacgg cgcacgattt tgaacaatgg gcttttgagt
    23821 taaaaggcag aacctttcaa tttgtaacag attctggtgt attttcacgt gaaacagtgg
    23881 attatggttc acgtgtcctg attgatgctt tcgattggga agagttacct gatgaaggaa
    23941 agatcttaga tgtgggatgc gggtatggac cgattgggtt agctttggca tttgcgacac
    24001 agcggtttgt tgaaatggtc gacattaatg aacgagcggt aagtctagcg caaggaaacg
    24061 caaagcgtaa ccaaatcgaa catgttgata tccatcaatc gaatatctat gaagcagttc
    24121 atgaagaaac ctatgcagct atcgtaagca atccgccgat ccgtgctgga aaaaaagttg
    24181 tacatgaaat tttgacaggc gcatatcctc ggttgaagaa gggcggtaca ctgacaattg
    24241 tgatccagaa gaaacaagga gcgccgagtg cgcaaaagaa aatggaagaa acatttggta
    24301 atgcagaaat cgtcacaaaa gataaaggat attatatcat caaaagtgtg aaagaataac
    24361 tagctgaaaa atagtggagc gacgaaagag tggaactggg cgtgcatgta cgggtacgta
    24421 tcagttcttt ttttgttgag aagtttttaa aaacaaagaa ctattttttc ttcaaaaaga
    24481 aatagtagga acagagtcgt tgttttttac gataaaataa ataagataca gaacttgtat
    24541 agaaggctgc caagtcggta gataaaggag gtttttatca aaaaacatac tttccttttt
    24601 tctctattct tttcttgggg gttttggtac actgataggg aatttagata gaagggagcc
    24661 aataggatga agctgaccat cagagatatc gcagaaatgg caggcgtatc agtaacaact
    24721 gtctcacaga ttttgaataa caaaggaagc agatttagtg ataagacaag aaaaaaagtg
    24781 ttgtcgatcg tgaatgaaca tcattataag cccgattatt tcgcttcaaa tgtgatcaat
    24841 cgacattcaa agaccatcgg aatgatcgtc cctgatgtca ctgatttctt cttctccaaa
    24901 ctgatcgaag gtgtagagag ctatttgaat cctttgggct atgtgatcat gttatgcaat
    24961 tctcgtcata gccaagaaaa tgagatcaag tatttacaag agctttccca ccgctcagtg
    25021 gacggaatct tgctggccac accaaatatt ttacctgaga aatatgcatt agacagcggt
    25081 ttttatcaaa aaatgccaat cattttaatc gatcgcggat tgaatcgtcg ggataacgga
    25141 cggcttatcg taaaggaata tgagggcgcc tatcaagctg tttcgctgtt tattgaaaat
    25201 ggccatacga agatcggcat gctgaaggaa accactggat actatcaatt agaagaacgt
    25261 ttcaacggat atcgtcatgc gctgaaagat catggcctgc cttttaatgg gaaatttgtt
    25321 gaacatggag agttgactgt ccaaggtgga tatactgcat caaaaaaatt actcaaacat
    25381 aaagatatca cggctatttt ttgcgggaat gatgccatgg cgatcggatg ctatcaggcg
    25441 atcgatgaat tgggcaaaaa aatcccagaa gatatctcag tcataggatt cgatggatta
    25501 aagctgtcag aatacatgat tccgcaattg acgacagtgc agcagccaag ttttgatatt
    25561 ggattttatg ctgcacgttt cctgatcgat acgatcgaat ttcctcaaag aaaagttccg
    25621 aacaaagtgt tcgaaacaaa attaattatt cgtgagagcg taaaagtatt gacaaagggc
    25681 taactcggcg gtatattgct tatgtaagca tttacaaagg ctagttagtg ctttttttat
    25741 ttttagtaaa ccagtttact aaagtggttt acctttccta aaaataatgt ataataccga
    25801 atgaagacaa ggaggatcag gcaatgcgaa tggtggatct gattgaaaaa aagagggatg
    25861 gaaacgagct gtcgaaagag gaaatcgagt acatcgtcac gaactatacg aatggaaaga
    25921 ttccagatta tcaagtcagt gcattattga tggcaatttt ttatcaagac atgacaaatg
    25981 aagaaatcac gaacttaaca ttggcgattg ctaattctgg tgatgtcatc gatttgagtt
    26041 ctcttgaagg aataaaggta gataagcatt cgacaggtgg cgttggtgac acgaccacat
    26101 tgattttagc ccccctagta gctagtgtcg gagtgacagt ggctaaaatg tctggacgcg
    26161 gtctaggtta cacaggtggt acactagaca agctagaagc aattcccgga tttcagatcg
    26221 agctttcgga tgaagaattt gtacgcattg ttaatgaaag taaagtagcg gtaattggtc
    26281 aatcaggaaa cttggcaccg gcggacaaaa aattgtatgc cttgcgtgac gtcacagcga
    26341 ccgttgattc tcttccgctg attgccagtt cgattatgag taaaaaaatc gcggcaggag
    26401 cagatgcaat cgttttggat gtgaccactg gagacggtgc tttcatgaag aacatcgaag
    26461 atgcaagacg tctggcaaag accatgacga gcattgggaa actggctaac cgagaaacag
    26521 tggcggttat ctcagatatg tcagaacctc ttggcgaagc tatcggaaat agtctagaag
    26581 ttgtcgaggc aatcgaaaca ttacaaggaa atggtccaga agatctagta gaaatgtgct
    26641 acgccttagg aagccaaatg gttgttcttg ctggcaaagc taagacaacc gatgaagcaa
    26701 gaacgttgct tcaagaagct ctggaatcag gtaaagcgtt agcaaaattc aaagaaatga
    26761 tccaaaatca agggggagac ccagcgatcg tggaacatcc agaacgaatt ttgactgcac
    26821 gctacacaat ggagcttcct gctaaacaaa gcggagttgt gtcaaaaatc gtggcaaatg
    26881 aattaggaat tgctgcaatg atgctaggtg ctgggcgtaa aaccaaagaa gaagatattg
    26941 atcacgctgt tggattgaaa ctgcataaaa aaattggtga cactgtgaca aaaggtgagt
    27001 cactattgac gatctatagt aacgacgaag aaatcacttc tgtcattgaa ttattatata
    27061 aaaatattga aattggtgaa tcggcaatga agccaacgtt gattcatgac atcatcacag
    27121 aataaaggaa aaaagcggac tttaccgttt taaagaaaag gggaaaagaa atggaactaa
    27181 accgaatgat cgaccatact attttgaaag cagacgcatc taaagaagct gtgatgcaaa
    27241 ttatcgaaga agcaaaaaaa taccattttt attctgtctg catcaatcca gcatgggttg
    27301 cgctagcgaa agaacaatta caaggtgagc cagtagccgt atgtacagta atcgggtttc
    27361 ctctaggagc aaacacaccc gagacaaaag catttgaaac aacagatgcc atcaataacg
    27421 gtgcagatga agtcgatatg gtcatcaata tcggtgcttt gaaatcaaag gatgatcaac
    27481 aagtccaaaa agatatcgaa gcagttgttg aagcagcaaa agatcgtgcg cttgtcaaag
    27541 ttattattga aacatcgtta ttagatagag aagaaatcat ccgagcttgc gaaatagcaa
    27601 aagctgcagg cgctgatttt gtcaaaacat caactggttt ctcaacaggc ggagcaaagg
    27661 tagaggatgt ccgtttgatg cgtgaaactg taggaccaga aatgggtgtc aaagcttccg
    27721 gcggtatcca taacgaagaa gaagcaatgg caatgatcga agctggtgct acaagaatcg
    27781 gaacaagcgc aggagtagca atcgtatctg gttcaactgg tgaaggctat taattttttc
    27841 aaaggagaag ctcatggcag aactcaaaca agaatggatc aacacagcta ttgaagcgtt
    27901 agataaagcc tatgttcctt attcccattt tccggtaggc gcttgtctcg taacggaatc
    27961 aggcaagatt tatcaaggca tcaacattga gaatgcatca tttggattaa cgaattgtgc
    28021 cgagcgcaca gcttttttca aagcagtatc tgaaggagaa aaaacattta cgcatctagt
    28081 cgtagcgggg cacacccccg atcctatctc tccttgtggt gcatgtcgac aagtaatggc
    28141 agaattctgt tcccctgata tgccggttac gctagtagga gacaatggtg tgacgaaggc
    28201 aacgaccgtt cgtgcactgc tgccttatgc atttactgaa aaagatctat aactcatgtt
    28261 cattcagtca gctgactgtt gaaaaataaa acaattattt taggaggctt tacagaaatg
    28321 aaaaaagcaa aattatttgg tttaggtgct gtcgcactag cagcagggtt atttttaggt
    28381 gcatgcggca acaacggctc tactgattca tccggtggca aaagctcatc agacacaaca
    28441 actgcagcat tgatcacaga cacaggtggt gtagacgacc gttcattcaa ccagtcagct
    28501 tgggaaggac tagaaaaatg ggggaaagat caaggacttt cacgaggaaa tgatggcttc
    28561 caatatttcc aatcttcaaa tgaatcagat tacatcccaa atattgacca agcattgaat
    28621 gctggattca aaacgatttt tggtattggt tataaactaa aaccagcgat ccaagaacaa
    28681 gcaacaaaca atacaggtac gaactttgtt attatcgatg atgtcatcga tggattagat
    28741 aacgttgtat ctgcaacttt caaagacaac gaagcttctt atcttgcagg tgttgcagca
    28801 gcttatacga ctgaaacaaa cgtagtcggc tttatcggcg gtgtaaaagg tgaagtaatc
    28861 gatcgttttg atgctggatt caaagccggt gttgatgcag gtgcgaaaga attaggaaaa
    28921 gaaatcaaag tattgaatca atatgctggt gacttctctg ctccagataa aggacgttca
    28981 attgctcaag gtatgtatgc acaaaacgca gatatcatct tccatgcttc aggcggtaca
    29041 ggtaacggtg tattccaaga agcaaaatca ttgaacgaat caggcgacaa aaaagtttgg
    29101 gttatcggtg ttgaccgcga ccaatcagac gaaggcgaat acacattaaa cggcgaaaag
    29161 aaaaacttca cattgacatc tacactgaaa gcagttggaa cagtagtaga agatttggca
    29221 caaaaatcag cggacggtaa attccctggc ggcgaacaca ctgtttatgg attgaaagaa
    29281 gacggtgttg gtctaacaga aggacaactt tctgacgaag cgaaaaaagc agttgatgaa
    29341 gcaaaagaaa aaatcatttc aggtgacgta aaagttccag aaactcctga agaaaactaa
    29401 aaccatttct atagccttgt tagacacatg tttgacaagg ctatggtttg attagaacaa
    29461 gaattgttca tttatccaag ctgccttgtg acagtttgga taagggaaca actccctgaa
    29521 gtaatgtttg tttcaaaaga agaacgctca ctaagcgtga atcttagtca atcgttgccc
    29581 tagatagaaa tcgttaggat gtgaaaaaaa ggtggttcaa gaaaatattg tcatagaaat
    29641 gcagaatatt accaaagaat ttggaacatt caaggcaaat gacaacatca atttacaagt
    29701 aaaggcagga gaaattcatg cgttgcttgg agaaaacggt gctggcaaat ctacattgat
    29761 gaacgtgctt tccggattat tagagccgac atcagggaaa attttgatgc gtgggaaaga
    29821 agtaaagatc acaagtccga ccaaagccaa ccaattaggg attgggatgg tccatcagca
    29881 ctttatgctt gttgatgcct ttactgtaac agaaaacatc gtgttgggaa gcgagcctag
    29941 tcgcgcaggg atgcttgacc ataaaaaagc acgaaaagag atccaaaaag tttctgaaca
    30001 gtatggattg tcagtcaacc cagatgctta cgttcgtgat atttcagttg gaatggaaca
    30061 acgggtagaa attttaaaaa cactttaccg aggagcagat gtactgattt ttgatgagcc
    30121 gacagctgta ttgacccctc aagaaattga tgaattaatc gtgatcatga aggaattagt
    30181 caaagaaggc aagtcgatca ttttgattac gcataagtta gatgaaatca aagcagtagc
    30241 tgaccgctgt acagttatcc gccgtggaaa aggaatcggt acagtcaacg ttaaagacgt
    30301 aacctcacaa caattagccg atatgatggt tggaagaacg gtttcattca aaacgatgaa
    30361 aaaagaagcg aagcctcaag aagtcgtttt gtctattgaa aatctagtgg tgaaagaaaa
    30421 tcgtggatta gaagccgtga aaaacttgaa cttagaggtt cgtgctggcg aagtacttgg
    30481 tatcgctgga atcgatggaa acgggcagtc ggagttgatc caagctttga ctggtttgcg
    30541 aaaggcagaa agcggacata tcaagctaaa aggggaagac atcactaata aaaaaccccg
    30601 aaaaatcact gaacatggtg taggacacgt gccagaagac cgtcataaat acgggttagt
    30661 cctagatatg acattatctg aaaatatcgc actacaaacc tatcatcaaa aaccttacag
    30721 taaaaacggc atgctgaatt attcagtaat gaatgaacat gccagagaat tgatcgaaga
    30781 atatgatgtt cgaacaacga atgaacttgt tcccgcaaaa gctttatcag gcggaaatca
    30841 gcaaaaagca attatcgctc gtgaagtcga ccgagatcct gatctgttga ttgttgcaaa
    30901 tccaactcgt ggactggatg taggtgcgat cgaatttatt cataaacgtc tgatcgaaca
    30961 aagggacaaa tacaaagcag tgttactgat cagtttcgaa ttagaagaaa ttttaaatgt
    31021 ttcggatcgt attgctgtta tccatgaagg agaaattgtc ggtatcgttg atccgaaaga
    31081 aacatctgaa aatgaattag gtctattgat ggcgggctat accttagaag aagcacgaaa
    31141 agagttagaa aagggggctg gcgcacatga atgatcgcag cgaaaaattc cgtaatttat
    31201 tagtgcctgt actttcagtt gttttaggat tgatcttagg cgcgatattg atggctgctt
    31261 ttggttttca gcccatccaa ggttattctt ctatgctaga tgcagccctt ggttcgcaaa
    31321 gagggatagg tgaaacgtta aggcaagcaa cgccgttgat ttttacagca ttaggttttt
    31381 ctgtagctaa ctcagctggg tttttcaata ttgggctttc tggacaagca ctttgcgggt
    31441 ggatcgtcag tgtctgggtg gcacttgcgt ttccagatct gccaaaagtt gttttgcttc
    31501 cgttatgcgt gatcttaggt gccttagctg gtgcgatggc agcggctgtt ccaggtttac
    31561 ttcgagcatt ttttggaaca agtgaagtga tcgtaacaat catgatgaac tatatcttat
    31621 tatacgtaag cacatttgct ttacatgaag tgatgcctga aagctatcgc tcaagtttgg
    31681 actcttccaa tatgatcagt caaaatgcct cactgcggtt accatggtta acacaagctt
    31741 ttggcggctc tcgcgtaaac tcaggattat ttttagcaat catcgcgctt attttcgttt
    31801 ggctgctgat gaagaaaacg actttaggat ttgaaatccg ttctgttgga ttgaatcctt
    31861 ttgcttctga gtatgcagga atgagtagta aacgtacgat cgttttgtcg atggtcattt
    31921 ctggcgcttt ggcagggctt ggcggtgtgg ttgaaggatt aggaacgtat caaaacttct
    31981 ttgttcaaac gacttcgcta tctattggat ttgacggaat ggctgtttcc ttgcttggtt
    32041 ctggatcagc aatcggtatt cttttatcag cgatcttgtt cagtattttg aagatcggtg
    32101 gacttggcat gcagacagga gcaggtgttc cgtttgagat cgtcaatgta tcgattgctt
    32161 tgattatctt ctttgtcggg atcaacttcc tgattcgttt cgtcatggct aaagtcttcc
    32221 aagggaaaaa acaagaagag atcgttgcga caatagaatc gaaaccaacg aatcaaaacc
    32281 atgaaggggg cgaactataa tgtcgactat cgaattaatt gccttgatcc ttacttctac
    32341 actggtttat tcaacaccgc taattctaac gtcactaggc ggaacttttt ctgaacgaag
    32401 cgggatcgta aacgtagggc tagagggaat catggtcatg ggtgctttta gttccatcgt
    32461 tttcaacttg acgatgagtc catcacttgg ttctatgaca ccatggatcg ctattttagt
    32521 gggtggcgtc gtaggtatcc tattttctct gattcacgca gtagcaacag tcagtttgcg
    32581 tgctgaccat attatcagcg ggacagtaat taatctgatg gcacctgctt taggtgtttt
    32641 cttgatcaaa gcatggtatg gtaaaggtca aactgataac atcacgcaga atctggggta
    32701 tttttctttc ccaggattag cagatattcc ggtcattgga ccaattttct ttaaaaatac
    32761 attcttacct gcttattgtg ctattttgat tgctgtccta gcttgggtga tcatcttcaa
    32821 gacgaaattc ggcttgcgcc tccgttcagt tggtgaaaat cctcaagctg ccgacacatt
    32881 aggtatcaat gtatatgcta tgcgttattc aggcgtactg atttctggct tgttaggcgg
    32941 tatgggcggt gctgtttttg ctcagtcgat ttccggtaac ttttcagctg gtacgatcgt
    33001 tggacaaggg ttcatctcca tggctgctat gatctttggt aagtggaatc ctttaggggc
    33061 aatgggtgcc tcattgttct ttggttttgc tcaaagcttg agtatcattg gcgcacaatt
    33121 accagttatt tcttcgattc cgccagtatt gctgcaaatt gctccatatt tgctgacaat
    33181 cattgtgctg gttgttttct tagggaaagc ttctggtcca aaagcaaacg ggaagaacta
    33241 tatcaaatca aaataatctt ttagcgaata aaaagggtgg gttacgggaa gtgtttttcg
    33301 tctcgccctt tttgttttaa aggcaataaa aataaaaaga aagaaggaat aatcatgttt
    33361 aaacgcgtac atctgatcgt catggattca gtaggaattg gagaagcacc agatgccgat
    33421 aagttcggtg atgttggttc tgatacatta ggacacattg cgaaagaagc aggattgaac
    33481 gttccacatt tagagcagct tggtttagga acgatccgtc ctcttgaagg tgtggaatca
    33541 gtgaaagatc atcaaggcta tgcgacgaaa ttagaagaag tttctgtagg gaaagatacg
    33601 atgacaggcc actgggaaat catgggattg aatatcaaaa ctccatttcg tgtgtttcct
    33661 aatggattcc ctgatgatct gatcaaacaa atcgaagaat tttctggccg taaagtcgta
    33721 tgtaacaaac cttatagcgg aacagctgtt attgacgatt acggtgaaca ccaaatgaaa
    33781 acaggcgatt taattgtata tacttctgcc gatcctgtcc ttcagatcgc ggcacatgaa
    33841 gaaatcattc ctttagaaga attatatcgg atttgccaat tcgttcgtga tataacaaaa
    33901 gatgatccgt atatgattgg ccgaatcatt gcacgtccgt acgtgggtga accaggagac
    33961 ttcacacgaa caagcaatcg ccatgattat gcgttgaatc cattcggaca cacagtcctt
    34021 gattctttga aagaagcagg aaaggacgtg attgctgttg gaaagatcaa cgatattttc
    34081 aacggacaag gaatcactga atctgtccgc acgaaaagca atatggatgg cgttgaccag
    34141 ctgctgaaag ttatgaaaaa cgatttttca ggaatcagct ttaccaattt agtagatttt
    34201 gatgcattat acggtcatcg tcgggatgta gtcggctatg cgcatgcgat tgaagaattt
    34261 gacttacgaa ttccagaatt gcttgaagcg atggaagaag acgacttgct aatgattaca
    34321 gccgatcacg gaaacgatcc gacgtttact ggtaccgatc atacgcgaga atatgtaccg
    34381 ttattggttt acagtaaaaa aatgaaagaa caaggggcat tgcctcaagg atattattca
    34441 gatatttcag caacgattgc tgagaacttt agtgtttctt cgactgaaaa tggcgaaagc
    34501 ttcttgaaat tattaaaata atctttttaa ataggagaaa gcaaatgacc aagttaagcg
    34561 agatgttaca agaaacgacc cagtttatcc ttgataaagg agtaaaagaa attgattttg
    34621 gtttgattct aggttcagga ttaggtgaat tagcagatga aatcgaagat gctgtaatga
    34681 tttcttatga tgagatcccg ttctttcctg tttcgacagt agttgggcat gcaggaaagc
    34741 tagtttatgg gtctctggca ggcaagaaag tgttagccat gcaagggaga ttccactact
    34801 atgaaggtca ttccatgcaa acagtgacct atccagtacg tgtgatggcc ggactagggg
    34861 cccattcagt cattgtgact aatgcatgtg gtggtgtcaa cgaatcattt gtaccaggag
    34921 atttgatgct gattaccgat catatcaatt ttactggtga aaatcctttg atcggtccga
    34981 atgaagatgc aatgggccct agatttccag atatgagtca agcttatacg cctgactatc
    35041 aagcaacagc aaaagcagca gcagaaaaac taggtttgga tcttaaagaa ggcgtatata
    35101 tgggctattc tggaccaact tatgaaacgc ctgcggagat ccgaatgtct cggacaatag
    35161 gagccgatgc agtagggatg tcgactgttc cagaagtcat cgtagcagct cacagcggat
    35221 taaaagtgtt aggaatctct tgtattacca acttagcagc aggtatgcaa gcgaatctga
    35281 gccatgaaga agttgtagaa acaactcaga gagtcaaaca atcttttaaa gcattggtta
    35341 aagaaacatt gacattattg taataggagg aacaataaaa tgagtgtgca tattgaagcg
    35401 aaagaaggcg agatcgccga taagatttta cttccgggag atcctttgcg agctaaatat
    35461 attgcggaga cttttttgga agatccagtt tgttacaatc aggtacgcgg tatgttaggc
    35521 tttaccggca aatataaagg agaacgtatt tctgttcaag gaacaggaat gggaatgcca
    35581 tcagccacca tctatgccca tgaattgatc cagtcgtatg gtgtaaagaa attgattcga
    35641 gtaggtacat gcggtgcatt gtcaaaagac gtccatgttc gtgatttagt actagcacaa
    35701 ggtgcagcga ccagttcttc gatgatcgaa aaaaatttcc aagcctttca ttttcctcca
    35761 atcagtgatt tcaatctatt actgaaagca tatgagatag ctaaagaaaa gggctatacg
    35821 gttcatgtag gaaatgttct ttcggaagat tctttctaca aagatgattt gacagagacg
    35881 ttccaattag ctgaacttgg cgttttagga gtggaaatgg aagcagcggc tctttattac
    35941 ttaggcgcga aataccatgt gcagacatta agtttgatga ccgttagcga ccatttgatc
    36001 accggtgaag aaacaacagc tgcagaacgt caatctacgt tcaatgaaat gatcgaagta
    36061 ggattggaaa cagcgatagc agaataagaa ggaaatgaaa aagctgccct gcattaagta
    36121 ataagaacat ccaagcaaaa atagctcctc atgtttttga cttggctgga cttattattg
    36181 caaatgtaag cttttgaaca ccgtttacca agaggttgtg aatctgccgt ttaactctga
    36241 gtcacaagga acaatttcaa aaa
//
LOCUS       DAFEOM010000216        18111 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.guided.055, whole genome shotgun sequence.
ACCESSION   DAFEOM010000216
VERSION     DAFEOM010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.guided.055"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            complement(134..577)
                     /locus_tag="LP524_002981"
     CDS             complement(134..577)
                     /locus_tag="LP524_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HBL2235946.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="LP524_002982"
     CDS             complement(795..2135)
                     /locus_tag="LP524_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL2235947.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="LP524_002983"
     CDS             2404..3300
                     /locus_tag="LP524_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBL2235948.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="LP524_002984"
     CDS             complement(3450..4391)
                     /locus_tag="LP524_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBL2235949.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="LP524_002985"
     CDS             4517..5638
                     /locus_tag="LP524_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBL2235950.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="LP524_002986"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="LP524_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBL2235951.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7426..7992
                     /locus_tag="LP524_002987"
     CDS             7426..7992
                     /locus_tag="LP524_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBL2235952.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="LP524_002988"
     CDS             8203..9264
                     /locus_tag="LP524_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBL2235953.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="LP524_002989"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="LP524_002989"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBL2235954.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="LP524_002990"
     CDS             10177..12159
                     /locus_tag="LP524_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL2235955.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="LP524_002991"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="LP524_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBL2235956.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..14305
                     /locus_tag="LP524_002992"
     CDS             12881..14305
                     /locus_tag="LP524_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL2235957.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14486..15259)
                     /locus_tag="LP524_002993"
     CDS             complement(14486..15259)
                     /locus_tag="LP524_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBL2235958.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15256..16161)
                     /locus_tag="LP524_002994"
     CDS             complement(15256..16161)
                     /locus_tag="LP524_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL2235959.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(16315..16635)
                     /locus_tag="LP524_002995"
     CDS             complement(16315..16635)
                     /locus_tag="LP524_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HBL2235960.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            17109..17795
                     /locus_tag="LP524_002996"
     CDS             17109..17795
                     /locus_tag="LP524_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBL2235961.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            17797..>18111
                     /locus_tag="LP524_002997"
     CDS             17797..>18111
                     /locus_tag="LP524_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 family protein"
                     /protein_id="HBL2235962.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLG"
BASE COUNT         5830 a         3264 c         3744 g         5273 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcgt tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    13381 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    13441 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
    13501 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
    13561 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
    13621 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
    13681 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
    13741 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
    13801 gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg
    13861 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
    13921 gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg cttacacact
    13981 tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc
    14041 ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt
    14101 atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc
    14161 aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca
    14221 cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt
    14281 tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc
    14341 tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc
    14401 tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt
    14461 tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta
    14521 atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat
    14581 gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga
    14641 tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga
    14701 ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa
    14761 agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag
    14821 tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa
    14881 tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat
    14941 aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact
    15001 ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg
    15061 cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt
    15121 caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa
    15181 caactaatag aacagataaa acagcagttg actttttctt gatcaattta aataattctt
    15241 gttttacaag caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag
    15301 agaagattcc aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga
    15361 caatagctgg atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc
    15421 aggatcttcc aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt
    15481 tgtagagata acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt
    15541 gatgatcttt ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag
    15601 taagtgactg gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg
    15661 cacatctctt gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga
    15721 cggatggttc agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga
    15781 tttcgctttt ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc
    15841 agatacatcg ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata
    15901 gatacccggg tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga
    15961 aatctcattt tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc
    16021 tttcatgatc gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg
    16081 ggataagcta aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc
    16141 ttggaccgaa agacttttca aaaatattca ctccttttct atagatagta caagcataca
    16201 cctgatgagt ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa
    16261 ttgaatatta aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg
    16321 gccgctagta atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt
    16381 ggaaagattc agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc
    16441 aatccctgtc cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa
    16501 aaagtaaaaa ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa
    16561 accatcactc catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa
    16621 ttccatccat gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa
    16681 aatcatccct tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat
    16741 tgtatatctt tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt
    16801 agtaaagtgt accaagcact gacggatata tcgttagcgg aaaagaagat gaatgctttg
    16861 caatttcata gaaagaagtt gttatttatt aatcatttaa aaaaaacagt aactagacct
    16921 tatttctata tattatcggg caataaatta aaaaaagctt taccttacaa taagtaagat
    16981 aatttttgtg ataaaaaaat aacgaaaaat atttaaaaat agtgtctatt tcgtgagaaa
    17041 acaactatat tttttatgtg ttttgcttta taattaaaaa aatattgtca ttttatggag
    17101 gaaaatatat ggaaaattat cttagccttt taggcgtctt gattgttatt ctcgggtttg
    17161 catttaaatt agactcgata ttgatcgtaa tggttgcttt agttgttaca gctttgactg
    17221 gcggtcttgg cattcaagga atgttagaac ttttaggtac aagttttgta aacaatcgcg
    17281 ggatggcgat ttttatcatc attatgttgg caacaggaac gcttgaaaga aatggtctga
    17341 aagaatcagc ggcttccttg atcaagcgat tcaagaaagt atctgctggt atgattgtag
    17401 atatttatgg gatctttcga atgatctttg cggcttttaa cgtgagtttt ggtggagttg
    17461 ctggatttgt tcgtccaatc attttaccga tggcattggg gaccatcgaa tcaaaagggt
    17521 tagaagtcca tcctaagcat gaagaagaac taaaaggtat ggcttcagct atggaaaatg
    17581 tctgctggtt ctttggccaa gtactattca tcggaggagc aggggcgttg ctagtccaat
    17641 caacactgaa agatctagga tatgacgtaa cattgggaca gcttgcttta gtggaagttc
    17701 cagtggctat cacggcttta gttgtggcaa gtgtctactt ctatcttaaa gaaaagaaat
    17761 tagcgaagaa atattatcca ggaaaggggc aataagatgg acttttttac aaatcctgat
    17821 attttattag gcgataaatt actggagatc ctttatatcg tgatgggact cgtattgatt
    17881 tacactggag taagaaattt aacagataaa accaatcctc atcgttatgg gtcagccttt
    17941 ttctggacgg tcttaggggt agttattgca ggaggaagat ggcttccggc aatcgtgaac
    18001 ggtggcttga tctttgcgat gaccatacca gcaattgcta agaaagtcag caaaggagaa
    18061 agccggcttc catcaaaaga atatatggaa aaaatgtcag acaaactagg a
//
LOCUS       DAFEOM010000217        16775 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.guided.059, whole genome shotgun sequence.
ACCESSION   DAFEOM010000217
VERSION     DAFEOM010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.guided.059"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            366..1229
                     /locus_tag="LP524_002998"
     CDS             366..1229
                     /locus_tag="LP524_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBL2235963.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            1329..1811
                     /locus_tag="LP524_002999"
     CDS             1329..1811
                     /locus_tag="LP524_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBL2235964.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(2074..2541)
                     /locus_tag="LP524_003000"
     CDS             complement(2074..2541)
                     /locus_tag="LP524_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBL2235965.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            2664..3515
                     /locus_tag="LP524_003001"
     CDS             2664..3515
                     /locus_tag="LP524_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL2235966.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(3766..5724)
                     /locus_tag="LP524_003002"
     CDS             complement(3766..5724)
                     /locus_tag="LP524_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL2235967.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(5721..6593)
                     /locus_tag="LP524_003003"
     CDS             complement(5721..6593)
                     /locus_tag="LP524_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL2235968.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(6935..7807)
                     /locus_tag="LP524_003004"
     CDS             complement(6935..7807)
                     /locus_tag="LP524_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL2235969.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            7935..8504
                     /locus_tag="LP524_003005"
     CDS             7935..8504
                     /locus_tag="LP524_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBL2235970.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            8571..9308
                     /locus_tag="LP524_003006"
     CDS             8571..9308
                     /locus_tag="LP524_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL2235971.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            9539..10807
                     /locus_tag="LP524_003007"
     CDS             9539..10807
                     /locus_tag="LP524_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBL2235972.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            11294..11758
                     /locus_tag="LP524_003008"
     CDS             11294..11758
                     /locus_tag="LP524_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL2235973.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            11742..12371
                     /locus_tag="LP524_003009"
     CDS             11742..12371
                     /locus_tag="LP524_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBL2235974.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            12725..14203
                     /gene="msr(C)"
                     /locus_tag="LP524_003010"
     CDS             12725..14203
                     /gene="msr(C)"
                     /locus_tag="LP524_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBL2235975.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            14250..14696
                     /locus_tag="LP524_003011"
     CDS             14250..14696
                     /locus_tag="LP524_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBL2235976.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(14895..15410)
                     /locus_tag="LP524_003012"
     CDS             complement(14895..15410)
                     /locus_tag="LP524_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL2235977.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            15527..16729
                     /locus_tag="LP524_003013"
     CDS             15527..16729
                     /locus_tag="LP524_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL2235978.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         5300 a         2989 c         3482 g         5004 t
ORIGIN      
        1 gatgaggttg aacttttaca caagattgtg gacttgactg caaagaaaaa tccaagcagc
       61 gttctgatca gcagtttgaa caacaatttg ctttgtacag taggtagtaa gcggattaac
      121 taagggaata aaagatattt ttaccgcttt gtttacttag aatctgtctt ccccaaaatt
      181 tatttttcta aaggtaaaac atagataggc agataatacc aattatccgt tacgaaacgc
      241 tgcttgagaa ttttctttag atccatacaa cattttctac atctgactaa attggttcct
      301 gattcatcaa gtaattgact ttctttgata atatgataaa aaggcaatca aggaggaaga
      361 taaatatgct taaagatctg aaccaattga tcgattacat cgaagaacat ttgacagaag
      421 gactttccta taaaataata gccgaaaata caggaatctc tgaatatcaa ttaaaacgca
      481 tctttacgtt tattgcaggg atgtcgctaa tggactatat caaaaaccgt agattggctt
      541 tggctaatcg cgatcttcta gatggatcaa gagtgacaga aacggcgttt agatatggat
      601 atgaaacagt cgaaggtttt tcaagagctt ttcgtgattg gtcaggatat ctgccttcag
      661 aaatcagcag atacggcata caaaaatctt ttccacgctt gacatttttt attgatgtca
      721 gaggaggaaa atcgatggaa tttcgtatcg aaaagaaaga acagttttat gtggcaggtg
      781 tggcgaaaaa agttcctgtc aaatttgaag gagtcaatca agaaatacaa aagttagcgc
      841 agacgatcac ggagactcaa agggaagaga tgcataagtt aggagattta tatccaaatc
      901 aagtaatcaa cgtttcttat ggatttgatg gagaccgctt ggaagaaaag ggcagtttga
      961 tgcacatgat tggctttgct acgacaaaag aaaatccgta cgctgattta gaaatgcttg
     1021 ctatagaaga acacacttgg gccatttttc ctaataaagg gccatttccc caaactttac
     1081 aagatacttg ggggaaaatc tatgcagaat ggcttccttc ttcagattat gggttagtcg
     1141 aagctcccga gatctctttt acaaaatggg gagaggattt cagtaatgtt tacagtgaga
     1201 tttggattgc agtgaaagca aaaaaatagt taaaaaataa aaatatccct agaaaaaaga
     1261 gcgtaatcgt gtcatttccc tttaatttta tgtaagatga acttaatata atagaaaggg
     1321 tgggagaaat ggcaaagtat aggatcgaag aaaaaccaga ttttacggtg acgggtattg
     1381 gtgtggaatt gaagagtagc aatacggatt ttgtcggttt ggctgcagag aaacaagcat
     1441 ttgagcaaaa agttatcggt aatggaacaa tagatgcatt gaaacaagct gctgaaaatc
     1501 attatttctt tacagtcaac gaaccctata acgggaaaat gatgtactat gtcggtgttc
     1561 aaacgagtca ggatgttcca aacactacgc gaatcatccc attcccaaaa ggaacgtatc
     1621 ttgttgtaag cgctttagca gaatcgcctg aagaattaag aaatgatttg acgaatacgg
     1681 cctttgggca tgttttaaga gaagtagaag attacgccta tgttgggggt ccaaatgctg
     1741 tagtcgaaat gggagttacc aacgggaaat atatgggaga aatctggata ccggtagtca
     1801 ataaaccatg ataatataga ggtcgtgaaa gtagcgcttt gacctgagca ttagagagga
     1861 aaaatgggaa atagctttcc atattttgca tttttcagaa ctaatgccga gggtcagcta
     1921 cttgaacacc gtttatcaag acgtcatgac ttcagcgctt tgacctgagc attagagagg
     1981 aaaaaggggc atgacaaggc ttttgtcccc cggagaataa aagaatttat gtagacgatt
     2041 ccgatcagaa aaatgcaggc acagtagaga gaattataaa aatctctcta cgaggcctgt
     2101 gttttctggt cttttttctt tttccaaaac ggctatttta tgtatcagac tatcaagtgt
     2161 gatcttagcc atttcttctt ctgccttttt ctgcagccga gcgtagcttt cttctaaagc
     2221 ttcttggata ttcgcaccaa cgacgcaatt tggatttgtt tttggatcga cttggataag
     2281 ctgtgtatta ccttcgatgc tgcgatagac atctagtaaa gaaatctgat ctggactttt
     2341 ggcaagggaa ggctgcggtt ttccggtttt tgtaaagatc aaaccggatt ttttgagctg
     2401 actcatgatt ttacgtacat ttgctgcatt tgtttcgata ctactggcaa tcatctcact
     2461 agaaagtgat cgggtatctt gaaacatttc aatataggca agtacatgga ttgcatcact
     2521 aaattgcaga gaatacttca tcagttgtca tcctttcatt ttttttgctt gacttatctt
     2581 accacactct ttatgataaa ggtgtaattt ataaatgtac agctgtaaat cttaacaaat
     2641 aaaaagaata gggagtggca aatatgcatt atttagtaac aggagcaact ggaggttttg
     2701 gtggttatgc gctggaattc ttaaaaaaga tggttccaca aaatgaaatc tatgtactag
     2761 tacgtagtga agaaaaagga gaagcactga aagaagctgg atttaatata cgtattggtg
     2821 attatgatga tttagactca atgaaagaag cgttgaaagg aatagatcga ttgctttttg
     2881 tctctggcgt tccaggaaat cgtcaagccg aacataagaa tgtagtgaag gcagcacaag
     2941 cagctggggt atcttatatc gcttatacaa gttttgcaga tgccgatcac tcgatcagtg
     3001 tattagcgcc agatcaccaa ttcactgaaa aaatgattaa ggattcaggt atttcccata
     3061 catttttacg gaataattgg tatgtagaga atgaattacc tatcattggg caggctttaa
     3121 aaactggtca attcgtttat gctgcaggaa atggaaaaac aggttgggca ttgaaacgtg
     3181 aatatgcaga agctgcagca aaagtactag tagaaagtga ctctccagaa atcttagaat
     3241 tatccggcaa tctaactgac tatgaagaat tagccaaggc attagagcgt gcgacaggta
     3301 aagagttaga aattattgaa gcaagtgatg cagcgtttgt cgaaaatcta aaagaagccg
     3361 gattccctca agaagcagct gacatgtttt tagcgatcca acatgatatt aaaaaggatc
     3421 agttagctat ttcctctgat gatttagaaa aagtattggg aaaaccatta acttctcttg
     3481 aagcagcatt aaaagaaata ttggatcagg aataatgtat aaagaggttg tgaaaaagcc
     3541 gattagtttt gagtatgaag aaaaattttg tgaaaaagct gttctgcatc aagtaataag
     3601 aagagtcaaa taaaaaatag ctcttcatat gtttcgttta gctggactta ttaccgcgga
     3661 gttggcagga gaacgccgtt tttattcgga aaaaggagct gagacaagat ttttgtctca
     3721 gctcctgttt gtttatcgat aaaaaagtct ggggaagacg atggctcagg ctttgttgga
     3781 aacagagccg attttatcat ttgcttttac atcacgtgcg ggttcgattt ctaagctttg
     3841 gactttgtcg ccatttgtga atacaacgac catatccgtg tttttgcctg cagattgaat
     3901 agcggcaaga tcaactaaag caatcaattg acctcttgcg actttttgac cttcttctac
     3961 atataaagtg aatggttcgc cttttaagtc aacagtgttg atgcccatat gaagcaagac
     4021 ttcgatacca gcatctgttt ggattccgac agcatgtttc gttgggaaaa tgtttgtgat
     4081 cgtaccagcg attggagaga agatttctcc attttctggt aatacagcat agccgtcacc
     4141 catcattttt tccgcgaata catcatctga aacttcagtg attggaatca gtttcccatt
     4201 tgcgatagcg taaatatctt gttcgatcgt ttcaccggca aatgtttctg ttttaaccac
     4261 ggtgtttgtt tgtgcaggtc gtgctgcagc accaccatgt agttttgcca tctcatctgc
     4321 aacgaattgt acttctgtac ctacgatgac ttgcgcattt tttccgtcga tgatcttcat
     4381 accaggtacg ccagtggctt tgattttatt ttgatcgatc aggcctgtat ctttgacttg
     4441 caaacgaagg cgtgtagtac agttatcgat cgaagttaca ttgtcggctc cgcctaatgc
     4501 agcgtagatt ttgcttgctt gtgccgcata tttattgtct gaattagtcg ttgcagtatc
     4561 gtcatctgaa agatctgctt cctcgcggcc aggagtcatc agatggaatt ttttgatagc
     4621 aaagttaaag ccaaagtagt aaatcactgc tgttactaat ccttggacga tcaacatcaa
     4681 tggctgattc gcaatcggat ttttcaaaga taggaagtaa tcgacaaatc cggcactaaa
     4741 ggcaaatcct gctgtccaat ggaagaatgc actcactgct aaagaaatcc cagtgaaaat
     4801 tgcatgcaag acatataatg gccaagcaac gaacataaat gaaaattcaa gtggttctgt
     4861 cacaccagta aagaatgaag caaaagctgc tgctagcatc aaggaagctg tttttgcttt
     4921 gttttctgga cgtgcatttc tgtaaatagc atatgctcct gctggaagcc cgaacatcat
     4981 gattgggaaa aatccagctt gatacattcc tgttacgcct tttgttcctt cagaagccaa
     5041 aaatttacca atatcattga ttcctgctac gtcaaaccag aacacggagt tcattgcttg
     5101 atgcaatcct gtagggatca gtaaacggtt gaagaagcca taaagacctg caccacccca
     5161 gcctaaactc aaaatgaatt tagcaaaaga aactaaagca gagtaaaccg gaggccatac
     5221 gagcagtagc actgcactga tcagcaacat gacaaaggca gccaagattg gcactagacg
     5281 cttgccgcta aagaaagata aagccatcgg taattttgct tcgtggaaac gattataaag
     5341 agctgccgca atcaatccgg caataatacc aataaatacg ttactgatct gagcaaaaga
     5401 tggatcaact gattctggtt tgatattcaa taaagtagct actgaatcag tttttaaaac
     5461 atttactgga acttcaaatg cgaccaaacc tgccaaggca gcagcacctg atttgtccac
     5521 tgacatccct agtgctaacc caacggcaaa cagcaaaggt aattgaccta aaactgcgtt
     5581 accgccagct tgtaaaaaat gagcgacaat gtcgttacta taagatgccc accagtttcc
     5641 gatccctact agtaaagcag cagcaggtaa gaccgcaact ggaagttgaa gggaacgtcc
     5701 cattcgttgt agatatgctt tcaatttttt tcctcctcat gattgttaac atttgtttgt
     5761 acgattgcgc ttcttaaacg ctcgatatgg atggccaagt aacccagttc ttctttcgtg
     5821 atcgcgagct ggtattcttt ggtcagcaac acacggattt ttttagaaat cttatagctt
     5881 tctgggtatt ttcgtttgac catttctacg atttccgtgt ctaaaacagc atattgttgc
     5941 gcatgggttc gttgtagaag tagtcgtaaa tgattcacca gtcttgaata attcagtgaa
     6001 aatggttctg aatggatatc tgtttccaat tctgattcca ctaaatggat gatgtcagat
     6061 acaatcgtca cttcacggat actgcgatgg ttattgttcc gaccgcttcg tccgctgtga
     6121 atatggattg cgatatatcc tgcttcgtca taagtatatg gaatcccaag ctcttttgtc
     6181 agatactcta ctgcccattg agcaatactg aattcatcac tgtaaagcac ttcgatttct
     6241 cttaaaagtt tgttgcggat gacgataccg ttattaatgt tctccgcaga aaaagcaatg
     6301 tggtcggtca aagcgatcaa tacatgttca ttcaactttt ccatcaaaac agtttccgca
     6361 tgatcaatga tcgtttctgc tgcaaataaa aagcgttcat caatctgatt aagtaaattt
     6421 tgcaacttga tttgaccttc tggttccatg acaaatatcc gttcgacatc acgatcaaaa
     6481 atcaaatcat ttctttttct gtcaaatcca atcccttttc caatagctac tttttcctgt
     6541 ccaccgtcat ctaccaatac tgcattttga ttaaagatct ttttgatttt catgcgcgca
     6601 ccctttttga agatttggtt ttcacgtttg ctagaccagt tgattgcggc caaacaaaaa
     6661 tagacacgaa aaacctccta gggaaaccat gttcctcaga gatttttcat gcctagtatg
     6721 atctagtcac atgaaacgaa ttattgttaa cgtaatcaat tttatataga cagttatgtt
     6781 ttgtcaattc tttttttata attatagtaa tttactggaa ttatcagaaa taaaaggaag
     6841 aaataaatta agaaacttat gttatttaat cgttggatct tttgcttatc ttattcgttg
     6901 ttaaaaagct gaaagcgaag gaaaaaaact ctacttaact gtatttctcc gttaggacat
     6961 ctaaaaattt acgagcagca ggagaaagag gctggtcttt tttccaaatg agattcagtg
     7021 aagcttgtaa tggcggatcg agtgggatga atattttttc cgtcccataa gtattgatga
     7081 tcccatctag agctaatgca ccaaaagtcc cgttatcgac catcaaagaa gcattgtaca
     7141 ataagttata tgttccgacg atcgataact gatctatgct gaaacctagc cattcagcta
     7201 gttgactgtc cactgacgtt tgccgggaaa tgatcaaagg aacagttttc agttcctgcg
     7261 gatggatgac ggtttgcttt gctaaaggat gatttcgatg tatcaaaagt ccccaacgat
     7321 cttgggctgg cagtcgaaat gactggtatt tttgtttgtt tgtcggttcg attacgagtc
     7381 caaaatcaag taatccgcta tccagtttgt ctatgatatc atctgcattc ccgctatata
     7441 aatgaaactg gatatcagga tgcgccttga tcaacgaatg aaggctatca gaaatgaaag
     7501 agattgcttt agtttcgcct ccgccaatgt agatttctcc agagatgatt gcgtcatttg
     7561 ttaaattgtt cgccgttttt tctaccaacg acaagatttc ttttcctttt gaataaagat
     7621 atcgaccaga atccgtcaac tgtatctctc gggagccccg agtgaaaagt gtcacaccaa
     7681 gttcttcttc taaatctttc agttgttttg agagagtagg ttgcgtgata tgcagagatt
     7741 ttgctgctcg gctgatattt ttttctctag ctactgctaa aaaatacgtc agtatacgaa
     7801 tatccatttt ttccctcctg tttttaaaaa agcataccac taagtatagt taaaaggaat
     7861 agttaagtat gttttataag tattagacat gataagccgt ccgttataaa gtaaggataa
     7921 aaggagggag caaaatgaca agcctcatcg aaaaacaaat cgtcaataaa gagattcgag
     7981 taacggatcc gctctttgaa gtgatccatc agatccaagc tgaaaacgaa gagcctttag
     8041 cagcgctgaa tactgggtac catgaaccag cagatatccg caaacggcta gaaaatatca
     8101 ttagtgacaa agtagatgat acggtgactg ttctgctgcc tttttataca gatttcggca
     8161 aacatattag tatagggaaa aatgtcttta tcaatcgtca agtgatgttc gttgacttag
     8221 gtggcatctg tttagaagat tcagtattga ttggtcctcg tgtcaacctc atcacggtga
     8281 accatctaac cgatccaaaa gaacgaagag gcttgtcagt aaagccaatc cacatcaaga
     8341 aaaatgcttg gatcggagca ggagcaacta ttctgccagg cgtaacgatt ggcgagaatg
     8401 ccattgttgc agcgaatgca actgttacga aagatgtccc agacaatacc atcgttgcag
     8461 gaatccctgc caaaatcgtc aaacctgtcg aacggattat ttgaacagta agatgaaata
     8521 agagatagtg agtataagca agagattttt caaaacagga ggagaataga atgaatgatt
     8581 tatcagaaaa agtgattgtc atcatgggcg cttcaagcgg aatcggtgaa gcaacaacga
     8641 agctattagc agaaaaaggg gctaaactag ttatagctgc acgccgagaa gatcgcctaa
     8701 aagcaataaa agaatcttta ccagaagctg agctatatat tcaaacagca gatgtaagag
     8761 attttgcaca agtccaagca gttatcgatt tagcgatgga aaagttcggt cgaatcgatg
     8821 tattgtacaa taatgcagga atcatgccaa ccgctccatt agttgaaggt caccgtgatg
     8881 aatggcaaaa tatgcttgat atcaacatca tgggtgtgtt aaatggaatt tccgccgtac
     8941 ttccaatcat ggaaaaacaa aaatctgggc atatcatttc aactgattct gtggcgggac
     9001 atgttgttta tcctgattcc gccgtttatt gcggaacaaa atttgctgtc cgtgcaatca
     9061 tggaaggatt acgtcaagag caacggcaaa ataacatcaa atctacgatc atctctccag
     9121 gagctgttca aactgaatta tatcaaacca tcagcgacaa aaaggctgct cttgaacttc
     9181 atgaagcgca aaaagaatgg ggattgacct ccgaagatat agcttctgct gtagcatttg
     9241 cgatagaaac gccagaccgg atgtctgtca gtaacatgat tatccgtccg acgacacagg
     9301 aagtataatc cataacggat aatatccaaa gagaattact tgttaaatag ttattcaaac
     9361 gaccatcaat ttaaggcgtg aatggagaag aatattcttc atttacgtct ttttatgtag
     9421 aaaaagcaaa ttcatcaaaa agaaacaaat gttatttaaa tatggacatt gacttgcttt
     9481 tactttttta gttacactgt tatttaggaa aagtttcata attcaaagga gaaaaaagat
     9541 gaaagcaaag aatttattat ttagcgttat tcttttagga aatctagcag ttgccaacac
     9601 aacagtgcta gctgaagaat cgacagaaac tgtcgcaact cagacatcga gttcttcaga
     9661 aacaggaatt gaaaacgata cagagagtac tttgcagtca gctactgtct cagaaaatac
     9721 aatttcaacc gaagaaccaa caactgaaac aacagaacaa acacaaaccg aagacagtac
     9781 atcagcagaa gctcagaaca atccagagaa tgcagaagaa atcacgaacc aattgacaga
     9841 tgggcaaaca ttgacacata cccatcaagc agatttgtct gaattcgaaa actatactgg
     9901 ctatgctttt tatgctcgct catctgctaa ttctcaacaa gcgttcattg atagtattgc
     9961 ctcaactgca caaagtttgg caagtgccaa tgacttatat gcatcagtca tgattgctca
    10021 agccatcgtt gaaagtggct gggggaacag tactttggct tctgccccta attacaatct
    10081 atttgggatt aaaggaagct ataatggcca atctgttacg atgcctactt cggaatatgt
    10141 gaatgggcaa tggattacgg tgaatgccgc ttttagaaaa tatccttcgt acaaagaatc
    10201 attgcaagat aacgtaacgg tattgaagac gacttctttt cagccaggcg tttactacta
    10261 ttccggtgca tggaaaagca atacgaactc ttacaaagat gctacggctt ggctaacagg
    10321 tcgttatgcc actgcgccta attatggttc tacattgaac aatgtaatcg aaacctataa
    10381 tctgacacag tatgatacag caccaacttc aacttctccg atgtatcgtc tgtataaccg
    10441 tcatacagga gaacatttat atacattgaa tgccggagaa aaaaattatc tgccaactgt
    10501 aggctgggaa tatgaaggga ttgcttggca agcaccaaac agcggacaac ctgtctaccg
    10561 tttatacaat ccttacagtg gagaccatca ttacacaatg gcacaatctg agatcaactc
    10621 tttaacaaaa atcggttggc gttatgaagg tctaagtttt tattctggtg gttccaagcc
    10681 aatttatcga ctatttaatc caaatgaaaa aactggtacc catcactata ccttgagtgc
    10741 taaggaaaga gattggctaa caccgatggg ctggagatat gaaggaatag gattttatgg
    10801 ttattaaaaa gaggtagtga aaaagctgcc ctgcatcaag tagtaagaac agccaaacaa
    10861 aaacagattt tcatgttttt tgtttggttg aacttattac cgcagatgca agtttttgaa
    10921 caccgtttac tcaaaaaagg ttgtgacaac atcttgtcat aactttttta ttactgttta
    10981 agcatactga tccaatttct aatattcgac gggattcttg caaaaggcga tttcggaaat
    11041 ttttggcggt gtaaaatttt ctactgcaga agagctatct tctatagttt gataagaatc
    11101 aataatcttg gttttagcgt tgtgctttct ttatcatttt ttccgttcat tctacaaaaa
    11161 atttcttttc tctatacttt tctattgaga gcgttttttt tattacacag ctgattcttt
    11221 gacttttttc tttttttatt ctattattga caagtcaatc gttgacgagt caattattag
    11281 aaaggaggaa attatgtctt tagaagagat tagtcacctt ttatatcaaa tcaaattagc
    11341 cgatcaaaaa atcgtccaga tctttgaaga aaatatcggt ataagtctta ctagatacga
    11401 gatcatgatg agactgaaag aagaaaatcc attgctacaa agcagtttgc aagaaaagtt
    11461 gcagattgat caaggagcag ttactcgtca tctgaaggta ttggaagcaa atggctatgt
    11521 ttcgcgtcag agaaatcctc agaacaaccg ggaagtgtat gtagcgctaa cagaaaaagc
    11581 ccagaagaaa atcgagcatt gtgaaatgga ccataaagat acaggttccg tgctatcccc
    11641 ggctttcagc gatgtagaaa tgaaacaatt tctaacactt ttagataaat tgaataccca
    11701 actttgcaat ataacaacag aaacgaaaga ggttaataat aatggaacaa aaaaatgatt
    11761 tttcaacaat tatgactggt agaagatccg ttcgcgtata tgatgaaaca tacaaaatcc
    11821 cacacgaaga aatgctggaa atgatcaaag aagcgacgac tgcaccttct tctgtcaata
    11881 tgcaaccatg gcgctttgtg gtcgctgaaa gcgaggaagc aaaagaaatc ttgcgtcctt
    11941 tgatccgttt caatacgaga caaaatgata cttcttctgc aatggtcatg atttttggcg
    12001 atatgcaatg ttacgaatat ggcgaagaaa tctataacca agcttacgaa tcagggaaga
    12061 tgccaaaaga agtcagagat caacaattag ctgcgatcat tccttattac aagagttttt
    12121 caagagaaga aatgaacgat gtagtcaaag tcgattctag cttagcggct atgcaattca
    12181 tgttagttgc gcgtgatcat ggctatgaaa caaatccaat tggtggattc gaagcagatc
    12241 agttggcaga ggcatttggt ttggataaag accgttatgt tccagttatt attttatcaa
    12301 taggaaaagc ggtagaagaa ggttacgagt ctgttcgttt agcaccagaa aagatcacta
    12361 cgttcgaata aaaggttttc agttactatt cagtagctat tgcttttttt gaatcgtgtg
    12421 tggtaaccta ataaaaaagg aggtcggaaa aatgactgca tcgatgaaat tacgtttcga
    12481 acttttgaat aacaactaaa taaattcaaa agacatgtat aaacaacaat ttcatggatg
    12541 gagtatgtcc attttttcga acgacagtag aataggtgaa agctatgaca gatttggtga
    12601 tttagtcata gttatttcgt tcagagtagg aaaagatgtg atgcagtttt tttgcaaaca
    12661 gatttttctg ctctttttta gtatcgataa ttgttgtttt acatgaaata ggagtgtaaa
    12721 acatatggaa aatttagcag taaatataac aaatctacaa gtcagctttg gcaaccagct
    12781 agaactatct attgattctc ttcgtgtcta tcagcaagat cgtataggga tcataggaga
    12841 aaatggtgtc ggtaaatcga ctttgctcaa actaatagcc ggtgaacttt ttcccgatca
    12901 tgggaaaatc caaacagaga tcaccttcaa ctacctgcct caattaacct atctcgctga
    12961 agcaaaggac ctaaatttgg aattagccag tcatttccag ttaagactgg aagaaacttc
    13021 agagcggaaa tggagcggag gggaagaacg aaagatcgag ttgatacgtc ttctttcttc
    13081 ttatgaacaa gggatgcttc tagacgagcc aacaacccat ctagatagaa aaagtattga
    13141 tcgactgatt gaagagcttc gttattatta tggtacgctg gtttttgtta gtcatgatcg
    13201 ctattttcta gatgagctag catcgaaaat ctgggaagta aaagacggag aaatccgaga
    13261 gttttcgggg aattatagtg cctatctcac tcaaaaggaa ttggagaaaa agactcagct
    13321 acgagaagca gagtcgatca tgaaagagaa aaaacgattg gaaaaatcga tccaagaaaa
    13381 gaaaaaacaa gcggaaaagt tggaaaaagt gtccagtaaa aagaaaaagc aacaaatcag
    13441 accggatcgg ttgtcttcct ctaaacaaaa agacagtgta caaaaagcca tccaaaagaa
    13501 tgcgaaaaca ttagagagaa gactccaaaa aataggagaa acaaccaagc cgcagcagat
    13561 gaaacaaatc cgttttccag taccaaaatc tcttgaactc cacagccgct atcccatcat
    13621 gggacaaaat gtccaattgg aaagaagtgg gagaacatta ctggtaaatg gtgattttca
    13681 gttttcttta ggtaaaaaaa tcgcgattgt cggtgaaaat ggttcaggta agacaacctt
    13741 attagaacat atccgcaaac aaggagaagg aatccttctc tctccgaaag taagctttca
    13801 agtatatcag caaaagggtt atcaaatgac atctgaagaa tccatcattc gttttgtcat
    13861 gagacaaaca gagttttcag aatcgcttgt ccgtagtttg ctgaatcact tagggtttgc
    13921 tcaggaaact ttgacgaaac cgttatgtac attaagtggg ggagaagcga cccgtctgac
    13981 gattgctttg ctttttacta agccaagtaa tgtgttgctg ttagatgaac cgactaattt
    14041 tattgatatg gcaacgatcg aagctttaga gaagctaatg caaatatatc cgggaacgat
    14101 tttgtttact tctcatgatt cttactttgt cgagcgtacg gctgatgaag tttatgaaat
    14161 aaaagggcag aaaataaaaa aagtacttac gagaaatttt taaatgtgct acgatgattt
    14221 tcgtgacaaa tttaaaggag tggtacagat tgcctacaaa taaaaaagaa ggaataattt
    14281 ttacaacaat gatgtgtttt ttgatggtct tggggatgag cgcgtataat ttatggctgc
    14341 atcaagatct tcatttatca agtttgttga ttggtctagt gcctggattc gtggtggctt
    14401 tcattttgga tgtcttcatc atcggtgtgc tggcgaaaaa aattgccttt aaactaccag
    14461 taaatccaga aagcaaggtc caaatgatct taactatttc atgcttgatg gttttaggaa
    14521 tggtgacatg tatgtcttta ttcggtgtga tcatggaaca aggaatacct gatcaattat
    14581 tctctgctta tatgaatgct tggaaaatga atgtgattgc cgctttaccg ttgcagttga
    14641 tcatcgttgg cccgctttca agaagtgtgc taaaagcaat tcaaagtaat gaatgatgga
    14701 acactgttat ttgaaaaaag gccttggcat gctttttgtc atagtctctt gaatgcagtt
    14761 ctagaaaaat agtgatccga acgataggtt ttggcctgtc gttcggatcg ctttttcttt
    14821 tccaactgta ttgctctttt attgaagata atttgctctt caatagatat ttctagaaag
    14881 gttctgttta tcccttactc aaaaaactgc tgcatttgtt ctcttacttg ctgacaccat
    14941 tggagttcct gctgacaaag cataattctg cgctggatca caacataacg tccaaattct
    15001 tgtttcttgt ctagcgagag gtcattcaat tttttttgat ttttttgcat gattttgttc
    15061 agctgttgtt ctctagtgaa tagcagtcca gtagctgttg ttttatcgag cgtcgaaaaa
    15121 gcatagattt ttgctagaaa ttcatctttt tgcgtaggag cgggagtttc ctctttgatc
    15181 cattcctcta caagagcacg tccttcatca gtcaagtggt agatcttttt ttgagagtgc
    15241 tcgtctttgt ccaaaacggt cacataaccc tgctggtcta acttggcaag cgttgtatag
    15301 atctgactgt gatgggcttc ccaaaaaatt gtgatcagct gcttcaattc atacccagaa
    15361 cgtggtttgg ataaaagcac gcataataat atataggcta aagtattcat aaaaatccta
    15421 cctctcgtct tattttcttt attatatcac agtaaagttt ccacgtttta catgtgcata
    15481 tttacatgta aaataaatca tgtatgatgc taatagagag aaagggatgg aaatattgga
    15541 gaatcaaata gatatacaag aacaaacaaa tgaagaaaca aaggggaaaa gttcagcgct
    15601 agttgtagca gctacctgta tgatcgcttt tatgggagtg ggtctagtag acccaatctt
    15661 aaaaacgatt gctgcacagt taaacgctac gccagctgaa acgaccctgt tatttactag
    15721 ttacatgtta gtcactggtg tggtgatgct atttacaggt ttcttatcta gtcggatcgg
    15781 tttgaaaaaa acattgatga caggattgtt tatcatcgtt gtattcgctg gtcttggcgg
    15841 attatctggg aatatcggaa tgctgattgg gttacgtgcc ggctggggtg tcgggaatgc
    15901 tttatttgtc tctactgctt tagcaacgat cgtcagtatt ttagttggta acacagaaaa
    15961 agcaatcatg atgtatgaag cagcacttgg ctgcggaatg gctgttggcc ctctagtcgg
    16021 tggggtcttg ggcagtggtt catggcgtta tccatttttt ggggtagcat ttttgatgtt
    16081 ttgcggtttt atagccctag cggtactttt acctgaaact gagaaaccaa agagaaaagt
    16141 aggcctttgg gaaggtgtca aagcgttaag caatcataaa cttcgttcag ttggactaat
    16201 cgcattgtta tataatttcg gattctttac tttacttgcg tatgcgccat ttttattgat
    16261 tggctattct gaattagaag tcggttttgt attttttggc tggggtgtgc ttctggctat
    16321 tgcttcgatt ttcgttgcac cggctcttga gcggaagttt acgacttatc ggacaatttt
    16381 agcagcattt gtcttattca ttatttgttt gctgttatta ggagctggaa cttctatgcc
    16441 agtccttgta ccgatcagca ttgtgttagc aggatttttc caaggaatta tcaatacact
    16501 gttgacgacg attgctatgg aaataccagg tctgcaacga aacgttgctt cgtccagcta
    16561 tagttttgtc cgctttttcg gtggagcttt agcaccattt attgcaggtg tgattggtga
    16621 acatattggt gaaagctatg cgttttattt tgcaggtgcc attgttctta tcagtgtatg
    16681 ttgtatcatt tatcaccgta attattttgc tacaaatgaa gaagaataag aaatcagagg
    16741 ttgtgaaaaa gctgtcctgc atcaagtcat aagaa
//
LOCUS       DAFEOM010000218         8062 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.guided.099, whole genome shotgun sequence.
ACCESSION   DAFEOM010000218
VERSION     DAFEOM010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.guided.099"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            128..1564
                     /locus_tag="LP524_003014"
     CDS             128..1564
                     /locus_tag="LP524_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL2235979.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="LP524_003015"
     CDS             1753..4200
                     /locus_tag="LP524_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBL2235980.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="LP524_003016"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="LP524_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBL2235981.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="LP524_003017"
     CDS             complement(5690..6793)
                     /locus_tag="LP524_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBL2235982.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="LP524_003018"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="LP524_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBL2235983.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2697 a         1375 c         1751 g         2239 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattacttc ttgaattttt gtgaaattcc
     7981 aacttatttc cgaagaagct acttttggaa caccgtgtac cagaggttgt gaaaaagtcg
     8041 cttagtttcg agtagtaaga aa
//
LOCUS       DAFEOM010000219         7780 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.guided.102, whole genome shotgun sequence.
ACCESSION   DAFEOM010000219
VERSION     DAFEOM010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.guided.102"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            245..1213
                     /locus_tag="LP524_003019"
     CDS             245..1213
                     /locus_tag="LP524_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HBL2235984.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1206..2237
                     /gene="vanA"
                     /locus_tag="LP524_003020"
     CDS             1206..2237
                     /gene="vanA"
                     /locus_tag="LP524_003020"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HBL2235985.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2243..2851
                     /locus_tag="LP524_003021"
     CDS             2243..2851
                     /locus_tag="LP524_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HBL2235986.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            3279..4190
                     /locus_tag="LP524_003022"
     CDS             3279..4190
                     /locus_tag="LP524_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HBL2235987.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            4343..4828
                     /gene="vanZ"
                     /locus_tag="LP524_003023"
     CDS             4343..4828
                     /gene="vanZ"
                     /locus_tag="LP524_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HBL2235988.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(5115..5444)
                     /locus_tag="LP524_003024"
     CDS             complement(5115..5444)
                     /locus_tag="LP524_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL2235989.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            5749..6078
                     /locus_tag="LP524_003025"
     CDS             5749..6078
                     /locus_tag="LP524_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL2235990.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            6068..6355
                     /locus_tag="LP524_003026"
     CDS             6068..6355
                     /locus_tag="LP524_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBL2235991.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(6634..7197)
                     /locus_tag="LP524_003027"
     CDS             complement(6634..7197)
                     /locus_tag="LP524_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL2235992.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT         2566 a         1298 c         1546 g         2370 t
ORIGIN      
        1 atgctggtgt catccaccaa catcaaaaag tatagagcca taatttttta ggaaaatctc
       61 aaggttatct ttactttttc ttaggaaatt aacaatttaa tattaagaaa cggctcgttc
      121 ttacacggta gacttaatac cgtaagaacg agccgttttc gttcttcaga gaaagatttg
      181 acaagattac cattggcatc cccgttttat ttggtgcctt tcacagaaag ggttggtctt
      241 aattatgaat aacatcggca ttactgttta tggatgtgag caggatgagg cagatgcatt
      301 ccatgctctt tcgcctcgct ttggcgttat ggcaacgata attaacgcca acgtgtcgga
      361 atccaacgcc aaatccgcgc ctttcaatca atgtatcagt gtgggacata aatcagagat
      421 ttccgcctct attcttcttg cgctgaagag agccggtgtg aaatatattt ctacccgaag
      481 catcggctgc aatcatatag atacaactgc tgctaagaga atgggcatca ctgtcgacaa
      541 tgtggcgtac tcgccggata gcgttgccga ttatactatg atgctaattc ttatggcagt
      601 acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat gatttcaggt tggacagcga
      661 ccgtggcaag gtactcagcg acatgacagt tggtgtggtg ggaacgggcc agataggcaa
      721 agcggttatt gagcggctgc gaggatttgg atgtaaagtg ttggcttata gtcgcagccg
      781 aagtatagag gtaaactatg taccgtttga tgagttgctg caaaatagcg atatcgttac
      841 gcttcatgtg ccgctcaata cggatacgca ctatattatc agccacgaac aaatacagag
      901 aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt ccacttgtag atacctatga
      961 gttggttaaa gcattagaaa acgggaaact gggcggtgcc gcattggatg tattggaagg
     1021 agaggaagag tttttctact ctgattgcac ccaaaaacca attgataatc aatttttact
     1081 taaacttcaa agaatgccta acgtgataat cacaccgcat acggcctatt ataccgagca
     1141 agcgttgcgt gataccgttg aaaaaaccat taaaaactgt ttggattttg aaaggagaca
     1201 ggagcatgaa tagaataaaa gttgcaatac tgtttggggg ttgctcagag gagcatgacg
     1261 tatcggtaaa atctgcaata gagatagccg ctaacattaa taaagaaaaa tacgagccgt
     1321 tatacattgg aattacgaaa tctggtgtat ggaaaatgtg cgaaaaacct tgcgcggaat
     1381 gggaaaacga caattgctat tcagctgtac tctcgccgga taaaaaaatg cacggattac
     1441 ttgttaaaaa gaaccatgaa tatgaaatca accatgttga tgtagcattt tcagctttgc
     1501 atggcaagtc aggtgaagat ggatccatac aaggtctgtt tgaattgtcc ggtatccctt
     1561 ttgtaggctg cgatattcaa agctcagcaa tttgtatgga caaatcgttg acatacatcg
     1621 ttgcgaaaaa tgctgggata gctactcccg ccttttgggt tattaataaa gatgataggc
     1681 cggtggcagc tacgtttacc tatcctgttt ttgttaagcc ggcgcgttca ggctcatcct
     1741 tcggtgtgaa aaaagtcaat agcgcggacg aattggacta cgcaattgaa tcggcaagac
     1801 aatatgacag caaaatctta attgagcagg ctgtttcggg ctgtgaggtc ggttgtgcgg
     1861 tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga ccaaatcagg ctgcagtacg
     1921 gaatctttcg tattcatcag gaagtcgagc cggaaaaagg ctctgaaaac gcagttataa
     1981 ccgttcccgc agacctttca gcagaggagc gaggacggat acaggaaacg gcaaaaaaaa
     2041 tatataaagc gctcggctgt agaggtctag cccgtgtgga tatgttttta caagataacg
     2101 gccgcattgt actgaacgaa gtcaatactc tgcccggttt cacgtcatac agtcgttatc
     2161 cccgtatgat ggccgctgca ggtattgcac ttcccgaact gattgaccgc ttgatcgtat
     2221 tagcgttaaa ggggtgataa gcatggaaat aggatttact tttttagatg aaatagtaca
     2281 cggtgttcgt tgggacgcta aatatgccac ttgggataat ttcaccggaa aaccggttga
     2341 cggttatgaa gtaaatcgca ttgtagggac atacgagttg gctgaatcgc ttttgaaggc
     2401 aaaagaactg gctgctaccc aagggtacgg attgcttcta tgggacggtt accgtcctaa
     2461 tcgtgctgta aactgtttta tgcaatgggc tgcacagccg gaaaataacc tgacaaagga
     2521 aagttattat cccaatattg accgaactga gatgatttca aaaggatacg tggcttcaaa
     2581 atcaagccat agccgcggca gtgccattga tcttacgctt tatcgattag acacgggtga
     2641 gcttgtacca atggggagcc gatttgattt tatggatgaa cgctctcatc atgcggcaaa
     2701 tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg cgctccatca tggaaaacag
     2761 tgggtttgaa gcatatagcc tcgaatggtg gcactatgta ttaagagacg aaccataccc
     2821 caatagctat tttgatttcc ccgttaaata aacttttaac cgttgcacgg acaaactata
     2881 taagctaact ctttcggcag gaaacccgac gtatgtaact ggttcttagg gaatttatat
     2941 atagtagata gtattgaaga tgtaaggcag agcgatattg cggtcattat ctgcgtgcgc
     3001 tgcggcaaga tagcctgata ataagactga tcgcatagag gggtggtatt tcacaccgcc
     3061 cattgtcaac aggcagttca gcctcgttaa attcagcatg ggtatcactt atgaaaattc
     3121 atctacattg gtgataatag taaatccagt agggcgaaat aattgactgt aatttacggg
     3181 gcaaaacggc acaatctcaa acgagattgt gccgtttaag gggaagattc tagaaatatt
     3241 tcatacttcc aactatatag ttaaggagga gactgaaaat gaagaagttg ttttttttat
     3301 tgttattgtt attcttaata tacttaggtt atgactacgt taatgaagca ctgttttctc
     3361 aggaaaaagt cgaatttcaa aattatgatc aaaatcccaa agaacattta gaaaatagtg
     3421 ggacttctga aaatacccaa gagaaaacaa ttacagaaga acaggtttat caaggaaatc
     3481 tgctattaat caatagtaaa tatcctgttc gccaagaaag tgtgaagtca gatatcgtga
     3541 atttatctaa acatgacgaa ttaataaatg gatacgggtt gcttgatagt aatatttata
     3601 tgtcaaaaga aatagcacaa aaattttcag agatggtcaa tgatgctgta aagggtggcg
     3661 ttagtcattt tattattaat agtggctatc gagactttga tgagcaaagt gtgctttacc
     3721 aagaaatggg ggctgagtat gccttaccag caggttatag tgagcataat tcaggtttat
     3781 cactagatgt aggatcaagc ttgacgaaaa tggaacgagc ccctgaagga aagtggatag
     3841 aagaaaatgc ttggaaatac gggttcattt tacgttatcc agaggacaaa acagagttaa
     3901 caggaattca atatgaacca tggcatattc gctatgttgg tttaccacat agtgcgatta
     3961 tgaaagaaaa gaatttcgtt ctcgaggaat atatggatta cctaaaagaa gaaaaaacca
     4021 tttctgttag tgtaaatggg gaaaaatatg agatctttta ttatcctgtt actaaaaata
     4081 ccaccattca tgtgccgact aatcttcgtt atgagatatc aggaaacaat atagacggtg
     4141 taattgtgac agtgtttccc ggatcaacac atactaattc aaggaggtaa ggatggcgga
     4201 atgaaaccaa cgaaattaat gaacagcatt attgtactag cacttttggg gtaacgttag
     4261 ctttttaatt taaaacccac gttaactagg acattgctat actaatgata caacttaaac
     4321 aaaagaatta gaggaaatta tattgggaaa aatattatct agaggattgc tagctttata
     4381 tttagtgaca ctaatctggt tagtgttatt caaattacaa tacaatattt tatcagtatt
     4441 taattatcat caaagaagtc ttaacttgac tccatttact gctactggga atttcagaga
     4501 gatgatagat aatgttataa tctttattcc atttggcttg cttttgaatg tcaattttaa
     4561 agaaatcgga tttttaccta agtttgcttt tgtactggtt ttaagtctta cttttgaaat
     4621 aattcaattt atcttcgcta ttggagcgac agacataaca gatgtaatta caaatactgt
     4681 tggaggcttt cttggactga aattatatgg tttaagcaat aagcatatga atcaaaaaaa
     4741 attagacaga gttattattt ttgtaggtat acttttgctc gtattattgc tcgtttaccg
     4801 tacccattta agaataaatt acgtgtaaga tgtctaaatc aagcaatctg atctttcata
     4861 cacataaaga tattgaatga attggattag atggaaaacg ggatgtgggg aaactcgccc
     4921 gtaggtgtga agtgagggga aaaccggtga taaagtaaaa agcttaccta acactatagt
     4981 aacaaagaaa gcccaattat caattttagt gctgaggaat tggtctcttt aataaatttc
     5041 cttaacgttg taaatccgca ttttcctgac ggtaccccca aactaagatt tcatagatag
     5101 attttctgat aactttaaaa aacacgaata gcggaaagga tgtcttcatt cgctaaagag
     5161 taataaacta cttttccatc ttgacgtgat tttgccattc catgtttttt taaaaaacgt
     5221 aaatggtgtg acgttgtagc gacactagca gtaagtattt ctgctaaatc acagacgcac
     5281 atttccttat aggtttctaa agcatagaaa attttaattc tagaaaaatc gctgaaacat
     5341 ttccctaaca caagtaagtt tgaaaaatct ttttgatcca atttattttt tattccattt
     5401 actttttctt tatgtacgac agtcacttgg caaacttttt tcattcatct cacctcacat
     5461 tcaaacgatt atttgaatga agtataccta tttgatcact cttttgtcaa tctaccttaa
     5521 ttccaataca ctattataag caatttcatg tattgaagta aacttacttg caagtgcgtt
     5581 tttgattcca agttaaaaac atattattat tctacataat ttcctaatca aataaaactc
     5641 ctttgaaaat tgttctaaag gagttttatt attttgtatt tctactaaaa atagcgtaca
     5701 atgtaattac taagtaatta cgaaatgtaa aataatggag gaaataatat gattacaaaa
     5761 acaagaaaac aaggaaattc tattatgtta acggtcccta aagaattcga tgtgcccaat
     5821 ggtgttgaag tagaagctaa gcttgtagag aatggtatcc tttatgagtt tgtagagcct
     5881 aaaaaagaat tttttgattt tagtgaagat attttggctg atattctttc agaaggttat
     5941 agcaaacaag agatcttaaa agaatttaga aatagaaaaa atgcgttaac ttctgcattt
     6001 aaatctattg ctgaagatac tgttgcaaat tctaaaccga tgacaaaaga ggagttggca
     6061 gcagaaattg gcttatgaga ttctaccttc taaacacgta ataaagtatc taaaaaaatt
     6121 aaaagagaaa acattaaaag aacaattttt aacgataatt tatgatgaga tagcagttcg
     6181 tcctcattcg ggagaacaaa aaacgggtga tttatcagga atttgggcta ttggattcaa
     6241 gtatgcaggt acgacatatc gcgttgcata tgaaattaaa gataatgcag tgatacctat
     6301 cttactatgt ggaacgcatg aaaattttta tgaacaatta aaaaagatta ggtaagaacc
     6361 caatgtttca acttgagagg atagacgaca ctttaaaaga taatttttaa cagctgagag
     6421 ttgtaatacc ctataatttt aaaccactaa cctatatatt tatgaaaaat agaaaaaatt
     6481 aaaacgcatt ttaagcctct ttcaaattaa aagcttcatc cttatgcaca aagagggctt
     6541 aaaatgcgtt attttatatt gttttttagt aataataaaa aaatcactct aattctccca
     6601 taaatattat agattcctaa agattaaaat agattattcc ctattgtctt ttaagtagcg
     6661 gtagagtgtt gccttgctaa taccagtcat ttccgtaatt tcttttacgc tataaacctg
     6721 acttttatac aattttactg ctcgatcaac atctttctgg ttcactcgtg gacggccacc
     6781 tttacgacct ctcgcacgtg cacttttcaa accttctttg gtacgttcaa caattaaatc
     6841 acgttcgaac tgactaaatg cttggaagac agtcatcatt aagcgtccgt gaggcgtgct
     6901 ggtatcgaag ttttctttca agctcaccag cttaacatgg tgttcttcaa aataggtaac
     6961 aaggtcaatc aaatcctttg tactacgacc caatcgagaa aaactttcta caattaccgt
     7021 atctcccgga cgaattttat cttttaaacg atttagttgg ggacgaaccg ctttggttcc
     7081 cgtcattttc tcagttagga tttcattaca attttgttca ttgagtaagt ctaattgacg
     7141 atttagttct tgtgaacgtg tactcactcg ggcataacca taaatgtatc cactcatgct
     7201 tttcttcctt tcgtacttta attctggctt gattgtatca taaaagggag ttatagatac
     7261 atagttaatg caacaagtta tgatacaaaa tatgacctct tttatttaat aaaagaggtc
     7321 atttcatttt gtctcataat cgattgtttt tgatacgagt gtgtatacga aataaacaaa
     7381 aaaatcttaa gtaaatttct agatacctaa tttaatccta actagtgtat cctaaagtta
     7441 ataacgagtt tatcacaatg aaaattataa aatgctccca tattagatat aacatgcaat
     7501 atttgaatta gaactacaaa atttattcgt tttataaagt aaataatgtt gagatactaa
     7561 ggaatagaat attataagga attgagctga atgtattgga taaaaaaaga ataactttat
     7621 tagagatgga ctataaaaat gctcgtaatt ttcttctgaa gtctgaaagc tatttgaatg
     7681 ctcagctacc agaatatatt agttttgacg aaactttaaa atctgctaaa aaagttttaa
     7741 cttcgccgtt tgtataatta agcgagacaa ataaaaaagc
//
LOCUS       DAFEOM010000220         6752 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.guided.110, whole genome shotgun sequence.
ACCESSION   DAFEOM010000220
VERSION     DAFEOM010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.guided.110"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            111..194
                     /locus_tag="LP524_003028"
     CDS             111..194
                     /locus_tag="LP524_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBL2235993.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            319..1056
                     /gene="erm(B)"
                     /locus_tag="LP524_003029"
     CDS             319..1056
                     /gene="erm(B)"
                     /locus_tag="LP524_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBL2235994.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1226..1486
                     /locus_tag="LP524_003030"
                     /pseudo
     CDS             1226..1486
                     /locus_tag="LP524_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1649..2314
                     /locus_tag="LP524_003031"
                     /pseudo
     CDS             1649..2314
                     /locus_tag="LP524_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2350..2595
                     /locus_tag="LP524_003032"
     CDS             2350..2595
                     /locus_tag="LP524_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL2235995.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2698..3567
                     /locus_tag="LP524_003033"
     CDS             2698..3567
                     /locus_tag="LP524_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBL2235996.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3548..4282
                     /locus_tag="LP524_003034"
     CDS             3548..4282
                     /locus_tag="LP524_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL2235997.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4315..5230
                     /gene="ant(6)"
                     /locus_tag="LP524_003035"
                     /pseudo
     CDS             4315..5230
                     /gene="ant(6)"
                     /locus_tag="LP524_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            5227..5769
                     /gene="sat4"
                     /locus_tag="LP524_003036"
     CDS             5227..5769
                     /gene="sat4"
                     /locus_tag="LP524_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HBL2235998.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5862..6656
                     /locus_tag="LP524_003037"
                     /note="aph(3')-IIIa"
     CDS             5862..6656
                     /locus_tag="LP524_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HBL2235999.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT         2366 a         1015 c         1407 g         1964 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaacca ttttgtgtat aataggaatt
       61 gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gggattcgtc atgttggtat
      121 tccaaatgcg taatgtagat aaaacatcta ctgttttgaa acagactaaa aacagtgatt
      181 acgcagataa ataaatacgt tagattaatt cctaccagtg actaatctta tgacttttta
      241 aacagataac taaaattaca aacaaatcgt ttaacttctg tatttattta cagatgtaat
      301 aacttcagga gtgattacat gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt
      361 gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga taccgtttac
      421 gaaattggaa caggtaaagg gcatttaacg acgaaactgg ctaaaataag taaacaggta
      481 acgtctattg aattagacag tcatctattc aacttatcgt cagaaaaatt aaaactgaac
      541 attcgtgtca ctttaattca ccaagatatt ctacagtttc aattccctaa caaacagagg
      601 tataaaattg ttgggagtat tccttaccat ttaagcacac aaattattaa aaaagtggtt
      661 tttgaaagcc atgcgtctga catctatctg attgttgaag aaggattcta caagcgtacc
      721 ttggatattc accgaacact agggttgctc ttgcacactc aagtctcgat tcagcaattg
      781 cttaagctgc cagcggaatg ctttcatcct aaaccaaaag taaacagtgt cttaataaaa
      841 cttacccgcc ataccacaga tgttccagat aaatattgga agctatatac gtactttgtt
      901 tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca
      961 atgaaacacg ccaaagtaaa caatttaagt accgttactt atgagcaagt attgtctatt
     1021 tttaatagtt atctattatt taacgggagg aaataattct atgagtcgct tttttaaatt
     1081 tggaaagtta cacgttacta aagggaatgg agataaatta ttagatatac tactgacagc
     1141 ttccaagaag ctaaagaggt ccctagcgcc tacggggaat ttgtatcgat aaggggtaca
     1201 aattcccact aagcgctcgg gaccccttgt aggaaaatgt cctaagtgtg gcaacaatat
     1261 tgtattaaaa aaatcgtttt atggttgttc aaattatcct gaatgtaagt ttactttagc
     1321 tgaacatttt agaaagaaaa aactaaccaa aacgaatgta aaagaattac tggagggaaa
     1381 agaaaccctg gtaaaaggaa tcaaaaacaa agagaaaaag ccctacaatg ccgttgtaaa
     1441 aattggggaa aagggatata ttgattttat ctctttttca aaataaacat ggggctgacc
     1501 caaaagtagc tcctatatga aaaatagagg tctttccatt tagatagtag tggaaagacc
     1561 tctatttatc ttttgaaagt gaatacaaaa ataaagcaca ctctgctata atataggtga
     1621 ttaatccatt tgagagaggt gctttatcat gtatcaaaat tataccataa accaactttg
     1681 tttgcctata gatcttgaaa ttaaattaga agaaaatgat tttgctcatg ccattgtgca
     1741 gtttgttgac tctattcccg atgaagtctt tcttccttat tatcaatcta tgggtagacc
     1801 ccaatatcat cctcgtatgc tactttccat tattctttgt gcctatattc aaggggttta
     1861 ttcgggccgc caaatacaaa atatgctgat agatagtatt cggatgcgct acttatctca
     1921 agagcaattc cctaactttc gtactattaa tcgttttcgc gttcatccga ttatgaacga
     1981 tattctagat cattcatttg ttcaatttcg tgagctactg gttcaaagtg gcttaatcag
     2041 tggcagtgct ctatacattg atggtacaaa aattgaagcg gatgcgaata aatactcttt
     2101 cgtttggaaa aaaagtattt taaagtataa ggatagcctt gatcaaaaag cccttgaaaa
     2161 ctaccaacaa atggtggaaa caaaaatctt acctgagtta atagatgatt taaaggatga
     2221 actttctata gaagagattg aaaggattcg tcaatctttg gaagaaaagg aaaatcaatt
     2281 aattgaagcg attgaccaaa ccgaaacagt agaagggggc tgacccatta ttaataagga
     2341 gtggacaaca tgattgatgt aaatgagcta ttggaagaag ctattagaga aactgaaaat
     2401 ctaaatgatg gcgaagtttt tcttgttaaa gatttattca agggatatgt ggggaatagg
     2461 atacccagaa aggatcgact tctgcttgga acgttatttt taaaccatgt tagcaaaatg
     2521 aatggtaatt taaaggctat tgaaaagact tcatctaatc agcagaggta taaaaagaca
     2581 attgataagt tttaactatc ttttgtgaaa ttaaaaaaat tataaatatg ttgagtagaa
     2641 gcaaaagatt tggcttggaa tttttacagc agtgaatcga taaaaggagc atgcttgatg
     2701 gtagataata ttattaaatc agtagcagag aaattatcct ctctgtctta tatagaaggt
     2761 attgttttag gtggttcacg tgcaaggggc acccatacag aggattcgga tatagatatc
     2821 ggcatctatt acaattcaga atcatttgac ataaatacta ttaatcaatt cgctacaaag
     2881 ctggatgatg agcatagaaa taaccttgtt gtacctcccg gagcatgggg tgattggatt
     2941 aatggcggcg gatggttagt cataaacggg tatcatgtgg atttaatttt acgtgatatt
     3001 aaacgtgtgg aacaaataat gaaagataca gagcacggaa ttgttactgc caattatcag
     3061 actgggcatc cccatggtta tattagtgca atgtatcgag gagaattagc gattagcaaa
     3121 atactatatg ctaagaatga aagcttatgc gaattaaaaa aacaggcaga aacttatccc
     3181 aatgctttgc agaaaagttt agttaacttt tttatgtttg aagcagggtt ctctttaatg
     3241 tttgtaaaag caaattcggg aacagacgat aaatattata ttgcgggtca tgtttttcgt
     3301 atagtttcat gtttaaatca agtgttattt gcatgtaata atgcttattg tatcaacgaa
     3361 aagaaagcta taaaactgct tgaaactttt gaacataaac ctgaaaaata taccgagaag
     3421 gtaaatcata tttttgaagt actcggtatc tcactttttg aatgctacga catgaccgag
     3481 aagctttata atgaagtgaa tgaaattgta tcggagataa ataacttttt aaacgaggag
     3541 agttcagatg aaagaaaaca aatatgatga taatatattt tttcaaaaat acagtcaaat
     3601 gagtcgctcg cagaaaggac tggctggtgc gggagaatgg gagactttga aaaagatgct
     3661 acctgatttt aagggtaagc gtgtgcttga tttaggatgc ggctatggat ggcactgtat
     3721 atatgcgatg gaaaacggtg cttcctctgt agtaggtgtt gatatttctc ataaaatgct
     3781 cgaagtagca aaaggaaaaa cccattttcc acagattgaa tatgaatgct gtgccataga
     3841 agatgtggat ttcccagagg agagctttga tgtaatacta agttcgcttg cgtttcatta
     3901 tgtagcagac tatgagaatt taataaaaaa gatatatagg atgctgaagg ctggtggcaa
     3961 tttagttttt acagttgaac atcctgtttt tactgctcat ggaacacaag actggtatta
     4021 taacgaaaaa ggagaaatac tgcatttccc ggtggacaat tattattatg agggcaaacg
     4081 gacagctatg tttttggaag aaaaggttac aaaatatcat agaacactga ccacatatct
     4141 aaatacactg ctttcaaata gttttataat aaatcagatt gtggagccac agccgccaga
     4201 gaacatgatg gatattccgg ggatggcgga tgaaatgcga cgcccaatga tgctgattgt
     4261 atcggcaaaa aagaagatgt aataatatag aaaaaataaa cgaggagtat gtaaatgaga
     4321 tcagaaaaag aaatgatgga tttagtactt tctttagcag aacaggatga acgtattcga
     4381 attgtgaccc ttgaggggtc acgcgcaaat attaatatac ctaaagatga atttcaggat
     4441 tatgatatta catattttgt aagtgatata gaacctagaa ccgtttatat ctaatgatga
     4501 ctggcttaat caatttggga atataataat gatgcaaaag ccggaggata tggaattatt
     4561 cccacctgaa gaaaagggat tttcctatct tatgctattt gatgattaca ataaaattga
     4621 tcttacctta ttgcccttgg aagagttaga taattaccta aagggcgata aattaataaa
     4681 ggttctaatt gataaagatt gtagaattaa aagggacata gttccgactg atatagatta
     4741 tcatgtaaga aagccaagcg caagggagta tgatgattgc tgcaatgaat tttggaatgt
     4801 aacaccttat gttattaaag gattgtgccg taaggaaatt ttatttgcta ttgatcattt
     4861 taatcagatt gttcgccatg agctgctgag aatgatatca tggaaggtcg gcatcgaaac
     4921 aggctttaaa ttaagtgtag gcaagaacta taagtttatt gaaaggtata tatccgagga
     4981 tttgtgggag aaacttttgt ccacctaccg gatggattcc tatgaaaaca tatgggaagc
     5041 attatttcta tgccatcaat tgttcagggc ggtatccggt gaggtggcgg aaaggcttca
     5101 ttatgcctat ccggagtatg ataggaatat aacaaaatat accagggaca tgtataaaaa
     5161 atacactggt aaaaccggct gcctggatag cacatatgcc gctgatatag aagagaggcg
     5221 ggaacagtga ttacagaaat gaaagcaggg cacctgaaag atatcgataa acccagcgaa
     5281 ccatttgagg tgataggtaa gattataccg aggtatgaaa acgagaattg gacctttaca
     5341 gaattactct atgaagcgcc atatttaaaa agctaccaag acgaagagga tgaagaggat
     5401 gaggaggcag attgccttga atatattgac aatactgata agataatata tctttactac
     5461 caagacgata aatgcgtcgg aaaagttaaa ctgcgaaaaa attggaaccg gtacgcttat
     5521 atagaagata tcgccgtatg taaggatttc agggggcaag gcataggcag cgcgcttatc
     5581 aatatatcta tagaatgggc aaagcataaa aacttgcatg gactaatgct tgaaacccag
     5641 gacaataacc ttatagcttg taaattctat cataattgtg gtttcaaaat cggctccgtc
     5701 gatactatgt tatacgccaa ctttgaaaac aactttgaaa aagctgtttt ctggtattta
     5761 aggttttaga atgcaaggaa cagtgaattg gagttcgtct tgttataatt agcttcttgg
     5821 ggtatcttta aatactgtag aaaagaggaa ggaaataata aatggctaaa atgagaatat
     5881 caccggaatt gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt
     5941 ctcctgctaa ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg
     6001 acagccggta taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc
     6061 tggaaggaaa gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca
     6121 atctgctcat gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa
     6181 gccctgaaaa gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca
     6241 tatcggattg tccctatacg aatagcttag acagccgatt agccgaattg gattacttac
     6301 tgaataacga tctggccgat gtggattgcg aaaactggga agaagacact ccatttaaag
     6361 atccgcgcga gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt
     6421 cccacggcga cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta
     6481 ttgatcttgg gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt
     6541 cgatcaggga ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga
     6601 tcaagcctga ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagtacc
     6661 tagatttaga tgtctaaaaa gctttaacta caagtttttt agacatctaa tcttttctgg
     6721 ctctttgtca aatgatgttg gtaaagagaa at
//
LOCUS       DAFEOM010000221         5562 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.guided.122, whole genome shotgun sequence.
ACCESSION   DAFEOM010000221
VERSION     DAFEOM010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.guided.122"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            345..1559
                     /locus_tag="LP524_003038"
     CDS             345..1559
                     /locus_tag="LP524_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HBL2236000.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            1590..1808
                     /locus_tag="LP524_003039"
                     /pseudo
     CDS             1590..1808
                     /locus_tag="LP524_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2227..2700
                     /gene="dfrF"
                     /locus_tag="LP524_003040"
     CDS             2227..2700
                     /gene="dfrF"
                     /locus_tag="LP524_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HBL2236001.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            2827..3483
                     /locus_tag="LP524_003041"
     CDS             2827..3483
                     /locus_tag="LP524_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HBL2236002.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            3665..4195
                     /locus_tag="LP524_003042"
     CDS             3665..4195
                     /locus_tag="LP524_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL2236003.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            4741..4983
                     /locus_tag="LP524_003043"
     CDS             4741..4983
                     /locus_tag="LP524_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBL2236004.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            4980..5450
                     /locus_tag="LP524_003044"
     CDS             4980..5450
                     /locus_tag="LP524_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBL2236005.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
BASE COUNT         1891 a          791 c         1145 g         1735 t
ORIGIN      
        1 aatcaattct gattcatcag tcccataaat tatagagccc aaatttgaga accgtagcga
       61 cactcgctgc ggttcttttt tatatatttt atatgaagta ttctttattc cttatgaaaa
      121 aatatcttta aaaataaaaa aagacgaaaa taaacggttt tctataaaaa gaagaccgtt
      181 tattttttgt ctttatttgt gtgaaaaacg aaaaagagca caacacatca agcttttatt
      241 aattgtctgt cttttataaa tgcaagtatt aattcaaaaa aacagaagaa aaaaacaact
      301 acaccttctt ttatactcgt aatcgtagaa aggaggcgaa aaaaatgaat acattaccta
      361 tcaatacagg agatacagca ttcatgattt tatgtacagc aatggtttgc ctgatgactc
      421 ctggtctagc ttttttttac ggcggattag cacggaagaa taatatacta acgatcatgt
      481 ctcaatcttt tctctccgtt gggataacaa cgattatgtg ggtatttggt ggattcggat
      541 tggcctttgg acgggatatc ggcggtgtta ttggaaaccc ggcagatttc tttttgatga
      601 gacatgttac aagcttcccc aacatgtttc atggtgcaac gattccattt ttaatgttct
      661 ttctttacca actgatgttt atcgtgatta ccgttccttt gatgacagga gcttttgcag
      721 ggagattgaa tttaaaaggc tatattatac ttgttatttt ttggaatctg ctgatttatt
      781 tccctgtttg ccactggata tggggtggcg gatttttgga tcagatggga tttagagatt
      841 tcgctggtgg agcagtcatc cacacgacag ctggattcgg ctctttagca tgtatcctca
      901 cacttggtca aagaaagatt cctttaggag aaagaagtca gcatagcagt cttatggcag
      961 ctgctatcgg tacaggactg ctttggtttg gctggttcgg ttttaattcc ggtggtgcac
     1021 ttcgagcaga tgaacaagca gtcaatgcat ttggttcaac attcatcgct cttgcatttg
     1081 cgatgattac ttggctgatc attgcaaaag taaaaggaaa tggctttgat tttgtcgacg
     1141 tactaacagg atccgttgct ggattggcag ccattacgcc ttgtgccggt tatgtagaag
     1201 caaaaagtgc catgctgatt gggatcattg cgggtatcgt ctgtcatgct gctgttgatt
     1261 ttcgtaaaaa gaaacaatgg gatgatgcac ttgatgtgtg gggtgttcat ggcatgggcg
     1321 gattcacagg tacgattctg atagggatct ttgcatctaa cagccattta ctaacgaatc
     1381 caagcagctg gtacttctta gggattcaag tgattggtgt catcattact gccgtgtatg
     1441 cctatgtgct gacaacagtg attttaaaat cggcgatgca ctttacaacg atcactacta
     1501 caaaagaaga acaagaagaa ggattggact ttttcttctt tggcaacaaa ctcaattgaa
     1561 acaaattcaa atagaaaaga ggaagattca tgcaacaaag caaaacgaaa attcctgcaa
     1621 aaaaattaac gttctttggc tttttggcta tgaccatctc aatggtcgtt tcattatatg
     1681 aatatcccac attcgcgacc tcaggcttca gcttagtatt tttcttgtta ttaggcggtt
     1741 tgctatggtt tatccctgtg gcattgtgcg cagcagagat ggcaacggtg aaaggatggg
     1801 aaaaaggtgt ttcccaaaca aaaagatatt aagaaacagc ccatgtttac ctgcttgcaa
     1861 acaaaagaat attgctacta gactaagaga tactacataa gtgtgttggt atctcttttt
     1921 tgttgccgtg aaacagcaga ctatttcatt tctgcttgat taccttaaaa cttattagtc
     1981 tatggtttgt caagggcttg ttgcgtgaga aatgctgaat atcacccttt acaaaacata
     2041 ggctgataag taacttttca aaagcagaaa agaaatggca atgcttataa caatatgata
     2101 taatgcagat gaagacaaaa aaggaatttg tcgagctggt tagttcgaga taattttgag
     2161 tttgttaaga tgtggtgcac ttgatggaat cacaggtacg acatcatatt atggaggatg
     2221 aaatagatga taggtttgat tgttgcgagg tcaaagaata atgttatagg caagaatggt
     2281 aatataccat ggaaaataaa gggagaacaa aagcaattta gagagttaac aacgggtaat
     2341 gtggttatta tggggcgaaa gtcttatgaa gaaatcggtc atccgttgcc taatagaatg
     2401 aatattgttg tttccaccac aacagagtat caaggagata atttagtttc agttaaatca
     2461 ttagaagatg cattattatt ggctaaagga cgagatgtat acatatctgg tggatatgga
     2521 ctatttaagg aagctttgca aatagtagat aaaatgtata tcacagaagt agatttaaat
     2581 attgaagatg gagatacatt ctttccagaa tttgatatca atgattttga agttttgata
     2641 ggggaaacac ttggtgagga agtgaaatat acgagaacat tttatgtaag gaaaaaatga
     2701 attgagtaaa ttttggattt agggtgtttt cataaatata ttgcttttta tgcatatata
     2761 aattgttgta agacaaattc cagtttgtat ggggcgataa aatcagaatt tgagagagga
     2821 ttgaatatga ataaggaatg gtctgaactt aataaaacaa tgcaggcaca aataaaaaag
     2881 aaagatactt ataagagggg tattgatact ttacttactt tacgaagtca gttaatacaa
     2941 accttagtat ctttcaaaga agaattatgc agagaagatt ttaactcaat ccccttcata
     3001 aacgctgatg gttatcatag taagacgatt gcttattcta tttggcatat tttccgcatt
     3061 gaagatatag ttgtgcatac agtgataaat gaagatgaac aagtattttt tgcaggcaat
     3121 tatcaagagc gtatcaattc acctatcatt acaacaggaa atgaactcat gaaacagcag
     3181 attgcagact tttcaaaaca gcttaatctg gaagaattat atttatatat tttcgaagta
     3241 tgggaaagca ctgagaaaat gcttgaacgg ttatcttatg atgaattgaa aagaaaaata
     3301 ccgaaagaaa gaaaaggata cttagaatcg ttgaatgtag taaacgacaa tgaaaaagca
     3361 atttggctga ttgattattg gtgtaataaa gatatttgtg gactaattca aatgccgttt
     3421 tcaagacatt ggattatgca cacagaagcc tgtttgcgta taaagaacaa gatacattca
     3481 taggcaaaaa tttcagtttg tagaactaag gaaattggaa tttaaggagg aataaagtat
     3541 ggttagtgat tttataagag cagcatttcc atggatttta atgggtttat ttgtagcaat
     3601 cagttgttcc cttatgagta aaaaagagaa ataaattcct gtttgtattt ataaaagaga
     3661 aaaaatgaga aaagtagttt tatttattgc catgagcctt gacggatata ttgcggacgg
     3721 taatggtgga gtagcttggc taaatggtca tggaaatgac aatgaaaaca tcgacactta
     3781 tacagaattt accaaagata tagatactgt cttaatgggg tggaatactt ctcatcaggt
     3841 tgttactgaa ctttctccgc aagaatgggt atataataaa tttacaactt atgtattaac
     3901 acataaagag tgcaattctt cccaacaaat tcattttaca agtgaaaatc ctgttttatt
     3961 attggaacgg ctaaaacaag aagcaggaaa ggatatttgg atttgtggcg gagcaaatct
     4021 tgttcagcag ttggtaagca aaaatatgat tgataaatat tacatttctg ttattcctac
     4081 tctgttagga aatggggttc gtcttttggg caacatagac agggaaataa agttaaggct
     4141 ttgtaaaaca cagacttata acggaataac cgatttgatt tatatgcgta gataactaga
     4201 gagaaacaga aaagctgtta aaccgacgga gcgatccgac cacgttggcg ggaaaatcct
     4261 taagattttt ccgccatgct tgcaaggggg cggttcgctt cggcggttcg gcgagcttgc
     4321 gagccgttca aagcccccgt tatctaacaa ggggggcaca aaaaacaaga aacactatac
     4381 actaacaagc ggaaaacgct gtatttataa ggcaaaaccc catttttaca gtagtgatat
     4441 aaattgtacc tatatcacta tgaaaattga atagtaaata tgagaaagac agttgatttc
     4501 aaaaaataag aaattgattg tcgttttctt ttgcagaaat ttaagtgcag gcaattatgg
     4561 tagttgtcgt aattgctgtc agctcagtgt ttatcactga tggtgtgaaa gatttacggt
     4621 tggagatgtt caataagatt attggtgctt aattattttt attgtagtgc tgatatgaga
     4681 atgttataat tggggtataa aacatacaaa taaatttgct gaaaaatgag gtatcaaaat
     4741 atggaaaatg aattactgga aaacttagga agacttcaca caactgaact tggcgttatt
     4801 agaattaaaa agaacttatc tttgaatgta gaaaatgtaa ttgaatggtg caaagaaaaa
     4861 attagccttt ctaatgcgga aatcacaaga aaaggaaaaa actggtatat aacgattgat
     4921 aattgtatta taacggtaaa tgcgtacagt tataccgtta tcactgctca caaggtaaaa
     4981 tgataaattt ttctataaag gaattaaacg catttgcagt tattgggcag gaagtagaac
     5041 tgactaatta tcaaaaaaag aatattcaga ttagtacaca gttttggaga aagtttaaca
     5101 gtagtttgaa aaaagcgtat ctttcacaat caggaaattg ggtaaaatat gcttttatgg
     5161 aaagaagaaa tggaaaactt tattatttct gttctattcc ccaaagaacc attaccccag
     5221 agggctttct gtataaagaa ataccgtctt ataaatattt ggttgtggag catattggtg
     5281 ctatggataa aatatatgaa acttatggca agatttatga agaaattatt cctagtacat
     5341 cgtatattcc tataaaagat attatcctac actttgaaaa atacgattat cgttttcatt
     5401 ggaatagtga taattcagtt attgaaattt ggatacctat tcaagattag aagcaatttt
     5461 atatccatac acacaaagca gttagtctta ggattgactg ctttttctat acagattttt
     5521 aaggctcttc gtcaatgagt gttggtgaag aaattcaata ga
//
LOCUS       DAFEOM010000222         3243 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.guided.146, whole genome shotgun sequence.
ACCESSION   DAFEOM010000222
VERSION     DAFEOM010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.guided.146"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            <1..1348
                     /locus_tag="LP524_003045"
     CDS             <1..1348
                     /locus_tag="LP524_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HBL2236006.1"
                     /translation="DFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQI
                     YKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAV
                     ILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKY
                     LIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNT
                     NLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLR
                     QMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFE
                     GKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGE
                     DILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(1478..2650)
                     /locus_tag="LP524_003046"
     CDS             complement(1478..2650)
                     /locus_tag="LP524_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HBL2236007.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT         1126 a          420 c          577 g         1120 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaaccgaa tcattaaaaa aacattatac
       61 agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg ttcctattgg
      121 atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt atccaaaaac
      181 tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt ggagtaaagg
      241 aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa gaaatgctaa
      301 tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat accaaaaatc
      361 tggttttaga attattgaag atttgccaga acatgaatta cacgagggca aaaaagaaga
      421 ttgttattta atggaatata gatatgatga taatgccaca aatgttaagg caatgaaata
      481 tttaattgag cattactttg ataatttcaa agtagatagt attgaaataa tcggtagtgg
      541 ttatgatagt gtggcatatt tagttaataa tgaatacatt tttaaaacaa aatttagtac
      601 taataagaaa aaaggttatg caaaagaaaa agcaatatat aattttttaa atacaaattt
      661 agaaactaat gtaaaaattc ctaatattga atattcgtat attagtgatg aattatctat
      721 actaggttat aaagaaatta aaggaacttt tttaacacca gaaatttatt ctactatgtc
      781 agaagaagaa caaaatttgt taaaacgaga tattgccagt tttttaagac aaatgcacgg
      841 tttagattat acagatatta gtgaatgtac tattgataat aaacaaaatg tattagaaga
      901 gtatatattg ttgcgtgaaa ctatttataa tgatttaact gatatagaaa aagattatat
      961 agaaagtttt atggaaagac taaatgcaac aacagttttt gagggtaaaa agtgtttatg
     1021 ccataatgat tttagttgta atcatctatt gttagatggc aataatagat taactggaat
     1081 aattgatttt ggagattctg gaattataga tgaatattgt gattttatat acttacttga
     1141 agatagtgaa gaagaaatag gaacaaattt tggagaagat atattaagaa tgtatggaaa
     1201 tatagatatt gagaaagcaa aagaatatca agatatagtt gaagaatatt atcctattga
     1261 aactattgtt tatggaatta aaaatattaa acaggaattt atcgaaaatg gtagaaaaga
     1321 aatttataaa aggacttata aagattgatt atataatata tgaaaagcta ttataaaaga
     1381 cattagtatt aaatagttta aaaaaatgaa aaataataaa ggaagtgagt caagtccaga
     1441 ctcctgtgta aaatgctata caatgttttt accatttcta cttatcaaaa ttgatgtatt
     1501 ttcttgaaga ataaatccat tcatcatgta ggtccataag aacggctcca attaagcgat
     1561 tggctgatgt ttgattgggg aagatgcgaa taatcttttc tcttctgcgt acttcttgat
     1621 tcagtcgttc aattagattg gtactcttta gtcgattgtg ggaatttcct tgtacggtat
     1681 attgaaaggc gtcttcgaat ccatcatcca atgatgcgca agcttttgaa tattttggtt
     1741 gatcgatata atcatgaatc aatcgatttt tagcctcacg cgctaagtta atatctgtga
     1801 acttaaaaat tcctttaaca gcttctctga aagattttga atttttttta ggaatggtgg
     1861 taaagatatt tcttaggaag tgaacttggc atctttgcca acttacgttg gtgaaggatt
     1921 ttctaatggc agagactaat cctttgtgcg catcagaaat aacgagttcc gtaccttgta
     1981 aaccgcgttc ttttaggtat tcaaaaaatg ttgtccaggt ctcttcgctt tcgccacttt
     2041 gaatcatgaa gccgataatt tcacggtcgc catctttggt tattccaatc gctatatgac
     2101 agctttttga gagtactcga ttttcttctc gtacttttat atagagtaca tcggtcatta
     2161 agtaaggata atttttttct gataataaac gattctgcca ctcgttaacc ataggttcta
     2221 gctgttctgt taagctagaa acgaaggact tagagacgga tttaccacaa agttcttcca
     2281 caatttttga tactttacga gttgaaacgc ctgatacata catttccaac attgaagcca
     2341 tgagggcttt ttcgtttcgt tgataacgtt caaacactgt gggtgaaaaa tggccatcac
     2401 gtgttctggg tacttttaat tctagcgtgc ctacacgtgt cgtaaagctg cgctcataat
     2461 agccatttcg ttgactttgt cggttttctg ttcgttcata ttcttttgct tgaatatatt
     2521 ctgttcgttg attttccatt agttgattaa ataccgttgt taaaatattt ttagaaacgt
     2581 catcctttac agaatattca ataatgcttt gaatctcttc gcttttcagt gtaaaatgta
     2641 cttgggtcat gtaaaagtcc tcctgggtat gtttttgtcg ttaaaaacat tgtaccgtaa
     2701 aaggactgtt atatggcctt tttactttta cacaattata cggactttat ctatataaag
     2761 gcgctgtcag agttttttga cttagaacat acattcattc aagagacttt aaacaagcta
     2821 gcagaacgcc caaaaacgga cacacaactc gatttgttta gctatgatac aggctgaaaa
     2881 taaaacccgc actatgccat tacatttata tctatgatac gtgtttgttt ttctgtactg
     2941 tttcctgagt gattagcaga aatataaaga ttaaaatttt aattgattat taggaagaga
     3001 aggagaatgt atcccgaaaa ctttttagtt ggcttggact gaacgaagtg agggaaaggc
     3061 tactaaaacg tcgaggggag tgggagcgaa gcgcacactt gatcttttga acttttttct
     3121 aatacttgac tttttttatg tatacagtta ctatgtatat agtaacactt tatacaatag
     3181 gactgtatat taagaggagt gtcttggttc tgttgcaaag ttttaaatct actatcaaat
     3241 aag
//
LOCUS       DAFEOM010000223         2242 bp    DNA     linear   BCT 22-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR31025_1
            SAMN20573933-rid13348413.guided.158, whole genome shotgun sequence.
ACCESSION   DAFEOM010000223
VERSION     DAFEOM010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20573933
            Sequence Read Archive: SRR16917227
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176950.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176950.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:16:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 28 of 66
            Pseudo Genes (incomplete)         :: 36 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 14 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20573933-rid13348413.guided.158"
                     /isolate="PR31025_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2018-10"
                     /collected_by="University of Michigan"
     gene            <1..148
                     /locus_tag="LP524_003047"
     CDS             <1..148
                     /locus_tag="LP524_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL2236008.1"
                     /translation="LIVDLKLCNRTNYAVKLYKEGNMTVNQICEITNVSRASLYRKLS
                     EVNN"
     gene            362..1057
                     /gene="vanR"
                     /locus_tag="LP524_003048"
     CDS             362..1057
                     /gene="vanR"
                     /locus_tag="LP524_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBL2236009.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1035..2189
                     /locus_tag="LP524_003049"
     CDS             1035..2189
                     /locus_tag="LP524_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL2236010.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVPIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          757 a          410 c          475 g          600 t
ORIGIN      
        1 tttgatagta gatttaaaac tttgcaacag aaccaattat gcggtaaagc tatataaaga
       61 aggaaatatg actgtaaatc aaatttgtga aattactaat gtatctaggg cttcattata
      121 caggaaatta tcagaagtga ataattagcc attctgtatt ccgctaatgg gcaatatttt
      181 taaagaagaa aaggaaacta taaaatatta acagcctcct agcgatgccg aaaagccctt
      241 tgataaaaaa agaatcatca tcttaagaaa ttcttagtca tttattatgt aaatgcttat
      301 aaattcggcc ctataatctg ataaattatt aagggcaaac ttatgtgaaa gggtgataac
      361 tatgagcgat aaaatactta ttgtggatga tgaacatgaa attgccgatt tggttgaatt
      421 atacttaaaa aacgagaatt atacggtttt caaatactat accgccaaag aagcattgga
      481 atgtatagac aagtctgaga ttgaccttgc catattggac atcatgcttc ccggcacaag
      541 cggccttact atctgtcaaa aaataaggga caagcacacc tatccgatta tcatgctgac
      601 cgggaaagat acagaggtag ataaaattac agggttaaca atcggcgcgg atgattatat
      661 aacgaagccc tttcgcccac tggagttaat tgctcgggta aaggcccagt tgcgccgata
      721 caaaaaattc agtggagtaa aggagcagaa cgaaaatgtt atcgtccact ccggccttgt
      781 cattaatgtt aacacccatg agtgttatct gaacgagaag cagttatccc ttactcccac
      841 cgagttttca atactgcgaa tcctctgtga aaacaagggg aatgtggtta gctccgagct
      901 gctatttcat gagatatggg gcgacgaata tttcagcaag agcaacaaca ccatcaccgt
      961 gcatatccgg catttgcgcg aaaaaatgaa cgacaccatt gataatccga aatatataaa
     1021 aacggtatgg ggggttggtt ataaaattga aaaataaaaa aaacgactat tccaaactag
     1081 aacgaaaact ttacatgtat atcgttgcaa ttgttgtggt agcaattgta ttcgtgttgt
     1141 atattcgttc aatgatccga gggaaacttg gggattggat cttaagtatt ttggaaaaca
     1201 aatatgactt aaatcacctg gacgcgatga aattatatca atattccata cggaacaata
     1261 tagatatctt tatttatgtg gcgattgtca ttagtattct tattctatgt cgcgtcatgc
     1321 tttcaaaatt cgcaaaatac tttgacgaga taaataccgg cattgatgta cttattcaga
     1381 acgaagataa acaaattgag ctttctgcgg aaatggatgt tatggaacaa aagctcaaca
     1441 cattaaaacg gactctggaa aagcgagagc aggatgcaaa gctggccgaa caaagaaaaa
     1501 atgacgttgt tatgtacttg gcgcacgata ttaaaacgcc ccttacatcc attatcggtt
     1561 atttgagcct gcttgacgag gctccagaca tgccggtaga tcaaaaggca aagtatgtgc
     1621 atatcacgtt ggacaaagcg tatcgactcg aacagctaat cgacgagttt tttgagatta
     1681 cacggtataa cctacaaacg ataacgctaa caaaaacgca catagaccta tactatatgc
     1741 tggtgcagat gaccgatgaa ttttatcctc agctttccgc acatggaaaa caggcggtta
     1801 ttcacgcccc cgaggatctg accgtgtccg gcgaccctga taaactcgcg agagtcttta
     1861 acaacatttt gaaaaacgcc gctgcataca gtgaggataa cagcatcatt gacattaccg
     1921 cgggcctctc cggggatgtg gtgccaatcg aattcaagaa cactggaagc atcccaaaag
     1981 ataagctagc tgccatattt gaaaagttct ataggctgga caatgctcgt tcttccgata
     2041 cgggtggcgc gggacttgga ttggcgattg caaaagaaat tattgttcag catggagggc
     2101 agatttacgc ggaaagcaat gataactata cgacgtttag ggtagagctt ccagcgatgc
     2161 cagacttggt tgataaaagg aggtcctaag agatgtatat aattttggct ctttgtcaaa
     2221 tgatgttggt aaagagaaat ct
//
